LOCUS       DAZKNM010000001        53657 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000001 DAZKNM010000000
VERSION     DAZKNM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.001"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..1668
                     /locus_tag="ACG8M9_000001"
     CDS             37..1668
                     /locus_tag="ACG8M9_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0376810.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1730..2371)
                     /locus_tag="ACG8M9_000002"
     CDS             complement(1730..2371)
                     /locus_tag="ACG8M9_000002"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0376811.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2557..2633
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2653..2961
                     /gene="rplU"
                     /locus_tag="ACG8M9_000003"
     CDS             2653..2961
                     /gene="rplU"
                     /locus_tag="ACG8M9_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0376812.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2988..3332
                     /locus_tag="ACG8M9_000004"
     CDS             2988..3332
                     /locus_tag="ACG8M9_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0376813.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3345..3638
                     /gene="rpmA"
                     /locus_tag="ACG8M9_000005"
     CDS             3345..3638
                     /gene="rpmA"
                     /locus_tag="ACG8M9_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0376814.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4072..6879
                     /locus_tag="ACG8M9_000006"
     CDS             4072..6879
                     /locus_tag="ACG8M9_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0376815.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7071..7562
                     /locus_tag="ACG8M9_000007"
     CDS             7071..7562
                     /locus_tag="ACG8M9_000007"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0376816.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7665..8654
                     /locus_tag="ACG8M9_000008"
     CDS             7665..8654
                     /locus_tag="ACG8M9_000008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0376817.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8687..9490
                     /locus_tag="ACG8M9_000009"
     CDS             8687..9490
                     /locus_tag="ACG8M9_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0376818.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9511..10425
                     /locus_tag="ACG8M9_000010"
     CDS             9511..10425
                     /locus_tag="ACG8M9_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0376819.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10508..10882
                     /locus_tag="ACG8M9_000011"
     CDS             10508..10882
                     /locus_tag="ACG8M9_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0376820.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11021..11266
                     /locus_tag="ACG8M9_000012"
     CDS             11021..11266
                     /locus_tag="ACG8M9_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0376821.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11438..12502
                     /locus_tag="ACG8M9_000013"
     CDS             11438..12502
                     /locus_tag="ACG8M9_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0376822.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12748..13173
                     /locus_tag="ACG8M9_000014"
     CDS             12748..13173
                     /locus_tag="ACG8M9_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0376823.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13166..13615
                     /gene="nusB"
                     /locus_tag="ACG8M9_000015"
     CDS             13166..13615
                     /gene="nusB"
                     /locus_tag="ACG8M9_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0376824.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13712..14566
                     /locus_tag="ACG8M9_000016"
     CDS             13712..14566
                     /locus_tag="ACG8M9_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0376825.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14567..15904
                     /gene="xseA"
                     /locus_tag="ACG8M9_000017"
     CDS             14567..15904
                     /gene="xseA"
                     /locus_tag="ACG8M9_000017"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0376826.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15909..16133
                     /locus_tag="ACG8M9_000018"
     CDS             15909..16133
                     /locus_tag="ACG8M9_000018"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0376827.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16135..17025
                     /locus_tag="ACG8M9_000019"
     CDS             16135..17025
                     /locus_tag="ACG8M9_000019"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0376828.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17034..17852
                     /locus_tag="ACG8M9_000020"
     CDS             17034..17852
                     /locus_tag="ACG8M9_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0376829.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17959..18408
                     /locus_tag="ACG8M9_000021"
     CDS             17959..18408
                     /locus_tag="ACG8M9_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0376830.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18426..20108
                     /gene="recN"
                     /locus_tag="ACG8M9_000022"
     CDS             18426..20108
                     /gene="recN"
                     /locus_tag="ACG8M9_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0376831.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20163..20279)
                     /locus_tag="ACG8M9_000023"
     CDS             complement(20163..20279)
                     /locus_tag="ACG8M9_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0376832.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20352..21308)
                     /locus_tag="ACG8M9_000024"
     CDS             complement(20352..21308)
                     /locus_tag="ACG8M9_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0376833.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21479..21847
                     /locus_tag="ACG8M9_000025"
     CDS             21479..21847
                     /locus_tag="ACG8M9_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0376834.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22017..22448
                     /gene="mraZ"
                     /locus_tag="ACG8M9_000026"
     CDS             22017..22448
                     /gene="mraZ"
                     /locus_tag="ACG8M9_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0376835.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22469..23428
                     /gene="rsmH"
                     /locus_tag="ACG8M9_000027"
     CDS             22469..23428
                     /gene="rsmH"
                     /locus_tag="ACG8M9_000027"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0376836.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23433..23834
                     /gene="ftsL"
                     /locus_tag="ACG8M9_000028"
     CDS             23433..23834
                     /gene="ftsL"
                     /locus_tag="ACG8M9_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0376837.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23835..26027
                     /locus_tag="ACG8M9_000029"
     CDS             23835..26027
                     /locus_tag="ACG8M9_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0376838.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26053..27015
                     /gene="mraY"
                     /locus_tag="ACG8M9_000030"
     CDS             26053..27015
                     /gene="mraY"
                     /locus_tag="ACG8M9_000030"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0376839.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27029..28390
                     /gene="murD"
                     /locus_tag="ACG8M9_000031"
     CDS             27029..28390
                     /gene="murD"
                     /locus_tag="ACG8M9_000031"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0376840.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28416..29504
                     /gene="murG"
                     /locus_tag="ACG8M9_000032"
     CDS             28416..29504
                     /gene="murG"
                     /locus_tag="ACG8M9_000032"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0376841.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29519..30739
                     /locus_tag="ACG8M9_000033"
     CDS             29519..30739
                     /locus_tag="ACG8M9_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0376842.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30895..32220
                     /gene="ftsA"
                     /locus_tag="ACG8M9_000034"
     CDS             30895..32220
                     /gene="ftsA"
                     /locus_tag="ACG8M9_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0376843.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32243..33484
                     /gene="ftsZ"
                     /locus_tag="ACG8M9_000035"
     CDS             32243..33484
                     /gene="ftsZ"
                     /locus_tag="ACG8M9_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0376844.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33501..34178
                     /locus_tag="ACG8M9_000036"
     CDS             33501..34178
                     /locus_tag="ACG8M9_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0376845.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34192..34791
                     /locus_tag="ACG8M9_000037"
     CDS             34192..34791
                     /locus_tag="ACG8M9_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0376846.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34872..35093
                     /locus_tag="ACG8M9_000038"
     CDS             34872..35093
                     /locus_tag="ACG8M9_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0376847.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35142..35924
                     /locus_tag="ACG8M9_000039"
     CDS             35142..35924
                     /locus_tag="ACG8M9_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0376848.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35945..36652
                     /locus_tag="ACG8M9_000040"
     CDS             35945..36652
                     /locus_tag="ACG8M9_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0376849.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36914..39712
                     /gene="ileS"
                     /locus_tag="ACG8M9_000041"
     CDS             36914..39712
                     /gene="ileS"
                     /locus_tag="ACG8M9_000041"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0376850.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39761..41287)
                     /gene="zwf"
                     /locus_tag="ACG8M9_000042"
     CDS             complement(39761..41287)
                     /gene="zwf"
                     /locus_tag="ACG8M9_000042"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0376851.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41302..41949)
                     /locus_tag="ACG8M9_000043"
     CDS             complement(41302..41949)
                     /locus_tag="ACG8M9_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0376852.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42133..42462)
                     /locus_tag="ACG8M9_000044"
     CDS             complement(42133..42462)
                     /locus_tag="ACG8M9_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0376853.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42639..43367
                     /gene="sfsA"
                     /locus_tag="ACG8M9_000045"
     CDS             42639..43367
                     /gene="sfsA"
                     /locus_tag="ACG8M9_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0376854.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43383..44396
                     /locus_tag="ACG8M9_000046"
     CDS             43383..44396
                     /locus_tag="ACG8M9_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0376855.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44396..45673
                     /locus_tag="ACG8M9_000047"
     CDS             44396..45673
                     /locus_tag="ACG8M9_000047"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0376856.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45736..48438
                     /locus_tag="ACG8M9_000048"
     CDS             45736..48438
                     /locus_tag="ACG8M9_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0376857.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48590..48907
                     /locus_tag="ACG8M9_000049"
     CDS             48590..48907
                     /locus_tag="ACG8M9_000049"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0376858.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48937..49257
                     /locus_tag="ACG8M9_000050"
     CDS             48937..49257
                     /locus_tag="ACG8M9_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0376859.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49365..50825
                     /locus_tag="ACG8M9_000051"
     CDS             49365..50825
                     /locus_tag="ACG8M9_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0376860.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50893..51114
                     /locus_tag="ACG8M9_000052"
     CDS             50893..51114
                     /locus_tag="ACG8M9_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376861.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51145..51327
                     /locus_tag="ACG8M9_000053"
     CDS             51145..51327
                     /locus_tag="ACG8M9_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0376862.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51327..51740
                     /locus_tag="ACG8M9_000054"
     CDS             51327..51740
                     /locus_tag="ACG8M9_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0376863.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51863..53044
                     /locus_tag="ACG8M9_000055"
     CDS             51863..53044
                     /locus_tag="ACG8M9_000055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0376864.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53115..53279)
                     /locus_tag="ACG8M9_000056"
     CDS             complement(53115..53279)
                     /locus_tag="ACG8M9_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376865.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53433..53506
                     /locus_tag="ACG8M9_000057"
     tRNA            53433..53506
                     /locus_tag="ACG8M9_000057"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53467..53469,aa:Arg,seq:cct)
     gene            complement(53575..>53657)
                     /locus_tag="ACG8M9_000058"
     CDS             complement(53575..>53657)
                     /locus_tag="ACG8M9_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0376866.1"
                     /translation="HIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        17714 a         9105 c        11605 g        15233 t
ORIGIN      
        1 tattttagaa aatttcatga aaaaggagtt agataggtgt tcaaaaatta tgtttcctca
       61 ctaaaaaaag aattttcagg ttatgatctt tcaagatttc agaaagatct tttggctggg
      121 atcacagtag ctgctgtagc attgccttta gccttggctt ttggggtatc aagcggagct
      181 gatgcggccg caggtttgat tacggctatc attgccggta tcgttattgg cggtttatca
      241 ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg cagctatctt gatgtctatt
      301 gcggcaacaa agggaatgca aggtgtattg cttgctactt ttttagcggg tctattttta
      361 cttgcagcag gacttttccg attaggaaca ctgacttctt ttatcccagc acccgtaatt
      421 acggggttta cttcaggtat tgctatcatt attgcattag gtcagatcga taatttattt
      481 ggtgttcact cggaaggtac aaacgttatt gaaaaactaa gtagttatca aacgatagga
      541 tttccgataa acacagcctc tttgatcatg ggagggctag tcattttagg gatgtttatc
      601 tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc tggctatcat tttggcaaca
      661 gcagttatgc tattgttcca tttaccagta gcaactgtgg gtaagattcc acaaacattg
      721 atcagtagta atcgtttgaa tatgggagat tttagctttt ctgccttgca acaagtggca
      781 gttccagcta ttagcattgc tttattagga atgattgaaa gcttgctatg tggtgcttcg
      841 gctggccgaa tggcaaataa gcccttagat agcaatcaag aattaatggc gcaaggaatt
      901 ggaaatctgt tgcttccttt ctttggcggg attcctgcta ctgcggccat cgcccgtaca
      961 agcgtggcca tcaaatctgg tgcacaaact agattagctg gtatgataca tgcagttgtc
     1021 ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac agattccaat gccagcactt
     1081 gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat gggaaacgat caaggagcta
     1141 tttactaata aatattggtc agcagttcta ttgtttatcc ttacgatgat ttgtacagtt
     1201 atctttgatt taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt
     1261 gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg attggcaaag aatggggctg
     1321 ccagaaacgg aaaagctgaa taactggaca gtagtgtata ttagtggtcc actgtttttt
     1381 atgtcttcag aaaaactcaa aaataaattg gaagaactaa atcatcacga tggtgtcatt
     1441 ttctccatgc gaggcgtacc aagtattgat ttgacggcac gtgcagtttt tgatgaattt
     1501 caagaaaaag cagagcaaag aggacaaacg attatctata cttccttgca gccagaagta
     1561 gaaaaacaac tttctcactt gtgggaacat acattcacgc atcaacatca aacagttgct
     1621 catgcgttga cagctttaca tgcgaaatat actgcaatta gccattaaaa caatgaaaaa
     1681 gaacagacaa gactgtaaac tgaagatttt atggtcttgt ctgttctttt taatattgct
     1741 gtaaaagtga agctagtact tgccagtctt gatggataac aggtacaagg gagcgattgt
     1801 agaaaatagc tgctggatga aaaagtggaa gaattcgata gtttttttct gaccattcgt
     1861 aggcatcctt ttgttctgtt aattgtaaga caggccctgt atagagttga ccatgatttt
     1921 tggtaaccgt atattctttg ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa
     1981 ttaattttgg ctggaccttc atcagttcgt aatccaatat tggtgcatgc gcaaatactt
     2041 cggaacgagt aggtgtccga tttggataaa cagtttctga ctgttgtgtc cgtttattga
     2101 tccgatgagt cacgcgatac gggcggctcc ttacagcact ggtgatatac acctcttctc
     2161 gtgttaagcc agttgaagaa agtgcctgca tcaattcttt tccagcttga ccgctaaaag
     2221 gaacatgcgt atcaatctct gttttccctg gggcttctcc cacgagcatc agtttaggat
     2281 gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg gccgttcatt cttaaagaag
     2341 cttcttgtat cagttctttt gggtattcca tttatgctcc tcctttttct tttattataa
     2401 gaataaaaga aacacaatgc gactatttta tttggtagaa ctgtgaggat agaaaagcag
     2461 aagtattgct tgaatttttg ttttactttt tgattttaaa tttagttgac taacggttgc
     2521 tgttctggta agctagtact gttgtaattg tggactccac agctacaacc gctcagaaca
     2581 agtaataagc acagcatatg ctgtcacttc ggatggcgag tctaagtata tcagaaggag
     2641 gtgctttata gcatgtacgc aattattaaa actggtggta aacaagtaaa agttgaagtg
     2701 ggacaagcaa tctatgttga aaaacttgac gttgaagcag gagaaaaagt tgtttttgac
     2761 gaagttatct tagtaggcgg agaatctaca aaagtaggcg ctccaactgt tgcaggtgca
     2821 actgttgaag gtactgttga aaaacacggc aaacaaaaga aagtcgttac tttcaaatac
     2881 aaacctaaaa aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt
     2941 atcgacgcga tcaacgctta atagcgtttg agaaagaagg cctatatatg atcaaaggaa
     3001 catttaagcg aaatgacgca ggtcagatcg tttcattcac tttgaccggg catgctgatg
     3061 ctggtcctta tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg
     3121 tcaacgggat tgcctcatta gccggttttg agccgattgt tgaaatgaat gaagaagaag
     3181 gcggttatct ttataccgaa gtcacttcag gaatgacaca agaacaaaac aatattgctg
     3241 agattctatt agaaaatctt ttattaggat tacaatcgat agaagctgaa aactcagaat
     3301 atatccaaat taaaacaatt aatgataaat aggaggtgca gaccatgttg ttatctatga
     3361 atctacaatt atttgcccac aaaaaaggtg gcggttctac atctaacggc cgtgactcag
     3421 aatcaaaacg tctaggtgct aaacgtgctg atggacaaac agttacaggt ggatcaattc
     3481 tttaccgtca acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg
     3541 acactttatt tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa
     3601 aacaagtgtc tgtttatcca gtagctcaag aagcttaatt gaatcttatg attaaagagg
     3661 ttgtgacaag acttttgtca cagccttttt tgtttataaa aataaaagtt gtagtggagg
     3721 ctacgaagtt tactttgtct ttttacactt aatatttttt tgccaatttg gaatcaggat
     3781 gttttgataa ctatcgtttt caattgatgg aagctatatt gtttgctatc agtgaaataa
     3841 ttatatggta aacagtgttc tttttcttgt ttccattata aaaatcgtat cgggattttt
     3901 cgttctttta aattgaacat ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt
     3961 aaatagttga cgaaacagaa ataaatacat agtattatgt tttgaagcct aagtacaata
     4021 gtctatagaa aaggagcgat accattggca aaacgcattg accgaattta tatgtatgtc
     4081 aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc aaggagtaac aacacaggaa
     4141 attgctgaag tgttagggat tcagcgaaca aatagcagta aagatctgaa tcagctagtg
     4201 cgcgaaggaa aactgataaa gacagacgga cgaccagtac gttacatata tcaaacactc
     4261 gttacgcacg taaagccagt tacaaaacat gtagtcagtt acaaagaaac gccggtctct
     4321 gaagaaaaaa atacactcca aatagatact aaggatatct ttgcaaaaat cattggagct
     4381 aacggcagca tgaaaaattc agtggaacag gcaaaagctg cgattttgta tccgccaaga
     4441 ggattaaatt gtttgattac aggacctacc ggatcaggaa agacttattt tgcacacgcg
     4501 atgtatcatt ttgcaaaagc gcatcatgta atagatgagg aaacggaact gattgttttc
     4561 aattgtgctg attatgccaa taaccctgag cttttgatga gtcatttatt tgggtacgca
     4621 aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga ttatcgatca agcggatggc
     4681 ggtatgctgt ttttggatga gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt
     4741 tactttatgg atcatggaac ttacagtcgt cttggggaaa caacgaaatc ccatgaagca
     4801 aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt cttctctatt agaaactttt
     4861 gttcggcgta tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag
     4921 aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa accgcacgca gcgaaagatt
     4981 tctttgacag aagatgtagc aaaagcattg atcggcagtg tgacctatgg aaatatagga
     5041 caattgaagt caaatatcca gttagtatgt gcacgaggat ttctaaatca tatgcaatca
     5101 cccgaaatct cgatcacaat cgatgatttg acagaaggga ttcgtagcgg attgattcaa
     5161 ttagcaagca accgcagtgc aatggcagag ctttctaaaa tattggagcc aaagataact
     5221 gtagcaccaa atgatacgat catgaaaatc caatcggact cctatgagtt accttataat
     5281 ctatatgata tcatcggaga taaagcagct ttactgaagt cggatggttt ggatcaagaa
     5341 gcaatcaatc atttcatttc tacagatatc aacatccatc tgaagtcttt ctataaagac
     5401 catggttttt cttttaatgc agacagcaaa ttagcagaat ttgtagatcc gaaaatcata
     5461 gaagtgacca atcagattta tgtaatggtc aaaagatctt taccatatga gtttcagcaa
     5521 aactttattt atgctatgag cttgcatatc agttcttttt tgaaaaaaat caacttagga
     5581 gaagaacggc atacgaatga taacatccgt gagatggctt ttgattttcc agatgaatat
     5641 gctgttgcga aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga gattccagaa
     5701 agtgaagact attacttgac cgttctactc gtctcgttac gggcaaatca agcaagcggc
     5761 agaatcggtg tagtcgttgc agctcacgga aacagcacag caagcagtat ggtccaagtt
     5821 gtccaacagt tgttagatgc agagaacgta cgagcagtag acatgccttt ggatatggat
     5881 ccgaaaacag cattggaccg aattgaacgc aatgtacaag aagtagatga aggaagcggg
     5941 gtcatactct tagtagatat ggggtccctt gcgtcgttta attcgcaaat tcaaagagac
     6001 actggaattc cagtacgtac tgttgatatg gtaacaactt ctctagtttt ggaaactgta
     6061 cgaaaggtct cagtattagg gacagatcta gatatgttgc atgaatcatt gaaaaatttt
     6121 cgaggttacg cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc tattcataag
     6181 aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa
     6241 ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat tagaagttct agctttgtcg
     6301 attgtggaag tgaaagaaga actgccagtt atccaaaaag aatatcagat catagctact
     6361 actgggatta ctgatttgaa tatccaagcc ccatttattc ctttagaacg ttttattgat
     6421 caaaatatcg aagtcatttt ggatcagttg ctgatggaat cagaattgga ggaaactgaa
     6481 ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat ctctgataac
     6541 ttcatcttta ttaatggtag taagttaatc gatccaatgt ggcaattttc tacacaaatc
     6601 tctcagacga cgggaatcgg cgatgaagaa tatggattca aaatcaacct agtgatgcac
     6661 acagcaggaa tgatcgaacg tattattcgg aatgaaccat tgactgtgga agaaaacgaa
     6721 ttgacaaata caacgaatga tcctctttat agccaattgg cagcctcagt tgttctgttg
     6781 gaagatcaga taaaagtaaa agtgccgata gaagaaatgt attatctgct acgtctagtc
     6841 cataatcagt tagataaaaa agaatacact gtgccatgaa tacacaaaaa cgatacactt
     6901 ttatagtgta tcgtttttgt gtattggtta agaatccttt aaattcaatt agtgtatcgt
     6961 aaaaaaagtt ggcacgcttt ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac
     7021 agaaatgata ggggtttctg tgaagtagtg aggaagaagg agttttgcag atggatattc
     7081 gattggcacg tatagatgat cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc
     7141 aaacaggtgt tgaacgtatc attgtgatca gcgatgtagt cgctcaagat aaattgagaa
     7201 agtttctact aaaagaagct gcaccgccgg ggatcacagc aaatgtaata accgtgcaga
     7261 aaatgattca aatctatcat agtggattat tatcacgagc aaaagtgatg cttttattca
     7321 ccaatcccca agatgttgaa gcggttgtcc gctgcggtgt acagttgaag tcgttgaata
     7381 tcggcggtat gagttttact gaaggcaaat caatgattac caattttgtt tcagtaaatc
     7441 aacaagatat tgattccttt cgttacttga acagtcaaga agtggagtta gagattcgaa
     7501 aagtacctgc tgacaggaaa atccttttga tggaactact agcgaaaatc aaaaaggtgt
     7561 agcaagaatg gttgttggaa aaggtcaaat aattctgttg taatggttag caattattaa
     7621 tatttttgaa tttgttacaa caaaagtaaa taggaggtat aaatatggta gggattatcc
     7681 tagcaagtca tggacaattt gctgaaggaa tcttacaatc cggttcaatg atctttggcg
     7741 aacaagaaaa cgtcaaagcc gtgattttga aaccaagtga aggtccagat gacttgagag
     7801 ccaaattgga agaagcggtt gcttcttttg ataatcaaga cgaagtgtta ttcttagttg
     7861 atttatgggg cggaacaccg ttcaatcaat caaacacatt attcgaagag cacaaagata
     7921 aatgggcaat cgtcagcggt ttgaatttac caatgttgat tgaagcctac gcatcacgct
     7981 tttcaatgga atctgctcat gaaattgcag cacacatcat tgaaacagca aaagacggtg
     8041 taaaagtaaa accggaagaa ttagaaccgg cagaagcacc gaaagcagca gttgaagacg
     8101 cacaacctaa aggagcactt ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg
     8161 tattagcacg tgttgactca cgtctgttac atggacaagt tgcgacagct tggacaaaag
     8221 cagtgcaacc aaaccgtatt atcgttgttt cagatgcagt atcaaaggat gatctgcgta
     8281 agagactaat cgaacaagct gctcctccag gtgtaaaagc aaatgttatt cctattagca
     8341 aaatgatcga agtagcaaaa gaccctcgtt ttggaaatac caaagcattg cttttatttg
     8401 aaaatcctga agacgtattg acagctgttg aaggtggcgt agacatcaaa gaattaaatg
     8461 ttgggtcaat ggcgcattcg gtcggtaaag tcgttgtaag taaagtattg tctatgggac
     8521 aagaagacgt tgaagcgttt gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta
     8581 aagtacctaa tgattcacgc gataacatgg atgatatttt gaaaaaagca aaagctgaat
     8641 tagcaaaagc gtaataaata atattaaaat ttataggagg cttatcatgt ctattatttc
     8701 aataatttta gtcgtattag ttgcctttct agctggtatg gaaggtattc ttgatgaatt
     8761 tcaatttcat caaccactag tggcatgtac attgatcggt ttagttaccg gtaacctcga
     8821 agctggtatc gtacttggcg gtacgcttca gatgatcgct ttaggttggg caaatatcgg
     8881 tgccgctgta gcaccagatg ctgcattagc atcagtagca tcagcaatta ttttagtttt
     8941 aggtggacaa ggtgttaaag gtgttccttc tgcgatcgcg attgccgttc ctttggctgt
     9001 tgcagggttg ttcttaacaa tgatcgtacg tacaatcgct gttccaatcg ttcatttgat
     9061 ggatgcggct gctgaagaag gaaacatccg taaggttgaa atgtggcaca tcattgctgt
     9121 atgtttacaa ggggttcgta tcgcggtccc agcagcagcg ttgctattta tcccagcaca
     9181 aactgtgcaa tcattccttg aatcaatgcc agcatggtta acagatggta tggctatcgg
     9241 tgggggaatg gtcgtagctg taggttacgc tttggtaatc aacatgatgg ctacaaaaga
     9301 agtatggcca ttcttcgtta ttggtttcgt tgtagctgca atttctcaat taacattgat
     9361 tgctctaggt gctttaggtg ttgcattagc tcttatttac ttgaaccttt ctaaaatggg
     9421 tggttcatca aataatggag gcggaagcaa ctctggtgac cctttaggcg acattttgaa
     9481 tgactattaa tcttgaagga ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa
     9541 aaagatcgtt tagctgttgc gtggcgctct accttcattc aaggttcatg gaactacgaa
     9601 cgtatgcaaa acggtggctg ggcattttct atgattccag caatcaaaaa attatacaaa
     9661 acaaaagaag aacgttctgc agcattaaaa cgtcacttgg aattctttaa tacacaccca
     9721 tacattgctt ctccaatttt aggggttaca ttggctctgg aagaagaacg tgcgaatggc
     9781 gcgcctgttg atgatgtagc gattcaaggg gttaaagtag gtatgatggg tcctttagct
     9841 ggtgttggtg acccagtgtt ctggtttaca gtacgtccaa tgctaggtgc actaggtgct
     9901 tcattagcta tgggaggaaa tatccttgga ccaatcatct tcttcttagg ttggaacatt
     9961 attcgttggg cattcatgtg gtatacacaa gaatttggct acaaagctgg ttctaaaatt
    10021 actgatgacc tttctggcgg attgttacaa gacgtaacaa aaggtgcttc aattttaggg
    10081 atgttcgtat tagctgccct tgtacaaaga tgggtatcaa tcaaattctt accagttgta
    10141 tcaactgtaa aattagataa aggtgcttac atcgaatggg ataaattgcc ttcaggtggt
    10201 gaaggaatca agagtgcatt tgaacaagtt aataatggct tagccctttc tcctgaaaag
    10261 gtaacaacat tacaagacaa cttagaccaa ttgattcctg gtttagctgc attaggatta
    10321 acattgttat gtatgtggtt gcttaagaaa aaggtttctc caatcgttat catccttggt
    10381 ttattcgtag tcggtgttgt tggacacgtc atcggacttt tataatccat aaaaatatag
    10441 ctcgggcgtt gtcccgagct ttttttatat agtatactgt tcataaacaa atatctggag
    10501 ggagaaaatg gttcaatcaa tcaatacaaa agtagacttg gtaattgatg ctacttcgtt
    10561 tacgggtctg acagattacg ggcaaatcat gataggtaat aatggttttg agttttacca
    10621 tgcgcgagat agacgcaaat atatccaaat tccatgggaa gaagtggatt atgtactagc
    10681 atcagtcatg ctaaagggaa aatggattcc tcgatatgcc attcaaacga aaaaaaatgg
    10741 tacatacaca ttttcttcca aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt
    10801 agatgaagat agaatggtac aatcattgag tttctttgat gtagtgaaac gtggtattcg
    10861 tagtatgttt aaacgcaagt aaaaaataaa gaaaacttct aagaggaggt tttctttatt
    10921 tttttacttt gcagaagaaa atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc
    10981 ccatatgcta aaattgatta gcaaagaagg aggttatatt atgagttggt tatggacatt
    11041 gattgttgga gccgtgatcg gagcaattgc aggtgcgatc accagtaaag gaaattcgat
    11101 gggctgtgta acgaatatca ttgctggact agtcggttca agtattggac aagcgatttt
    11161 cggcgaatgg ggaccaaaag ctgcaggtat ggcacttatt ccatctattt taggcgcagt
    11221 gattttggta gcagttgtct catttttctt tggaaaacga tcataagttt ttacaaaata
    11281 gtaaactaaa aggagttgca gcgagactgt gactcctttt tggctttatt tatttaaaat
    11341 aaaaagaagt tttccggaaa tttttattta actgtcgtgt acgaagaaaa gtttgctata
    11401 atggaaaaaa gcgtgcaaag aaagagggag tttcattatg atgctacgtg tagaaaaatt
    11461 gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg gttaccagtc cgtacaatct
    11521 tcgttactta acgaacttta caggtacgac aggattagct gttattactt tggaaaaagc
    11581 attttttatt actgattttc gttataccga gcaggcggca gcgcaggctc aagggtttga
    11641 aattattaaa aatgtcggac ctatttttga tgaggtagca aacttagtac gcaaagaaga
    11701 actgcactca ttagcttttg aggaaacaac agtctcattt ttagaatatt cggttttaga
    11761 agaaatcatc gatgcggaat tagtacctgt ttcacagatg atcgaagaac tcagagaagt
    11821 gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt catattgcgg atatggcata
    11881 tgatcacatc ctgaaaatga tccaaccggg catgactgag attgaagtcg caaatcaact
    11941 agacttttat atgcgttctc ttggcgcttc aggtgtttct tttgatacga tcgttgctag
    12001 cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga
    12061 cttgatcaca ttggactttg gttgctatta cgaagggtac gtttccgata tgacacgtac
    12121 attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc
    12181 tcaattagca gttattgatg ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt
    12241 tgctagagat tatatttcaa aacatggtta cggggaagca tttggacatt ctacaggaca
    12301 tgggatcggt ttagagatcc acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt
    12361 tgtaccagga aatgtgatca ctgatgaacc agggatctac ttaccaggaa tcggtggagt
    12421 acgtattgag gatgacttgt tgatcacaga agatggaaat cgtgtgttga ctcgttcacc
    12481 aaaagaattg atcatcttat agtttcatca atagaagaag taagcgtaaa aatcagtgtt
    12541 cacagacttt gttatagaaa atcactcgat ttaatgatac actatagaag aacttttaag
    12601 ctgtaagaga taataaagtt gggtgttttc tccaattcat agcagtttgc agctgatcgg
    12661 cggcagtttt cttaccacgt gatcactatg ccgcatcata gagaattcgg atgtatctcg
    12721 tgcttaggca aagggaggaa taaataaatg gcagatgaaa aaaatttgat tttaaacgca
    12781 aaccaagatt taggagagat cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt
    12841 gcagcttcta aagtagaagg cgtatacgga atgcgcggaa cttttgccag caatgtcact
    12901 gaattattag gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga ggaagatggc
    12961 ttgaaagtag atctttactg ttatctagaa tatggtgttt cagttcctaa agtagcaatg
    13021 gaaatgcagg aacgggtcag ccagcaagtc cttttcatga cggatatcga tttatctgaa
    13081 gtcaatatcc atgtagtagc tgttgttcct gaaaaattag aacaaccaac aatcgaagaa
    13141 ctttttgaag acgaagagga agagaatgaa taaagaatta tcaagacacg agattcggga
    13201 aatggcattg caggctttat ttccattaga cttcaatgca gacctaacaa aagaagatgc
    13261 gattttcaac gcaatcgaat tagaccaccg cgacatgatc aacgaagatg aatcagagtt
    13321 tgttcctgtt tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga
    13381 taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc aatcggatct caaaaatgga
    13441 tttggtgatc ttgcgtatcg cgatttttga aatgacttat gtgacagatg taccagcggc
    13501 tgttgcttta aatgaagcaa tcgagttggc aaaaacattt agtgatgacc gttcacgtaa
    13561 attcgtcaac ggtgttttat ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct
    13621 agcttttttc tatgaatcat cataaacctg ttataaatcg ggaaaattct atggtagaat
    13681 gaaaacacaa aaaatatctg ggagtggtta gatggcagaa ttgattaacg gaaaagaatt
    13741 agctgaaaaa atgcaggcag agattgctga aaaaatccaa aaattgaaag ataatggtat
    13801 ccatcccgga ttagtcgttt tattggtagg ggaaaatcca gccagccaaa tctatgtaag
    13861 aaataaagaa cgtgtagcaa aagaaatcgg aatccattca ttggtagagc gttatcctga
    13921 gacgatttct gaagacgaac tacttgcaga aattgaaaaa tacaatcaag atcctagttt
    13981 tcatggtata ttggtccaat taccattgcc aaagcatata gatgaagaaa agatcctgtt
    14041 agctatccgc cctgaaaaag acgtagacgg ttttcatccg atgaatttag gcagactcct
    14101 tgcgggtaaa ccggacaaga ttccttgtac tccatacggc atcatgaaaa tgtttgaagc
    14161 ttattctatc gatccggcgg gtaaacgagc agtagttatc ggacgaagca atatcgtcgg
    14221 taaaccgatg gctcagctac tcttgatggc agatgcaact gttactgttg ctcattctaa
    14281 aaccgaaaat ctggctgaac tggcacaaga agcagatatt ttagtcgtag caataggacg
    14341 tggacatttt gtcacaaaag aattcgtaaa accaggtgct gtagtgatag atgtagggat
    14401 gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa tttgatgaag tggagcctat
    14461 tgcaggctgg atcactcctg tgccaaaggg tgtagggcca atgactatca cgatgctgat
    14521 gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg cacaagaata
    14581 tttaactgtc actacattaa cgaagtattt gaaaagaaaa tttgatgccg atccttattt
    14641 agaacgcgta tatttaacag gcgaaatatc gaatttccgt ttacgtgcca atgcccatca
    14701 atatttcagc ttaaaagatg accatgcaaa aatatctgct attatgttca aaggagcatt
    14761 ccaaaaatta cgattccagc caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc
    14821 attatatgaa gcgggaggtt cttatcaaat ctatgtagag cacatggagc ctgatggaat
    14881 aggtgcttta tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt
    14941 tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc
    15001 aagcggagcg gttatccgcg atatcatcac gacggtgaaa agaagatatc caattgtcca
    15061 attggttttg ttcccaacag ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa
    15121 tatccagaga gtagaagaga tgggcaattt cgatacaatg atcatcggtc gtgggggtgg
    15181 ttcgattgag gatttatggc cgtttaatga agagcgggtt gctcgggcga tttatcaagc
    15241 taatacgccg gtgatttcat ctgtaggaca tgaaacagat accacgatcg ctgatttagt
    15301 agcagatgtt cgggctgcca ctccaacagc agctgcagaa ttagctgttc ctgttttgtc
    15361 agaagagttg atgcgcattc aagaacggca agctagattg cagcaggcgt ttgtacgcca
    15421 gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc
    15481 tgaaagattg tatgaaggtc agaccatcaa attagaccag ttgaaacagc gattatatca
    15541 agctacacag caaatttatc atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt
    15601 ggaacaagtg gctccgcttt atcgagttaa agcagctcgc caagaaactg cttatctaga
    15661 aaaacgctta gaagagaaaa tccaacaata tatgaaacaa cagcagcagc gttttgctca
    15721 agctgttcaa tctcttgatt tgttgagtcc attgaaaatc atgggaagag gctacagcta
    15781 tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag attcatcaag gggatttgct
    15841 gacgatccat tatcaagatg gaatcgttaa aggacaagta caagagatag acaaaggaga
    15901 ataaaaatat ggaaaatcca acttttgaag agtcactgca agaattagaa aaaattgtca
    15961 tgcagttaga acaaggagac gttccattag aatcagctct agattcgttt aaacgaggaa
    16021 tggaattaag caaacattgc caagatacat tagctaaagc agaagagacc ttgacgaaaa
    16081 tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga
    16141 ttaactgact tttcagctat tcatctacct gctgtagaaa aagaaatctt gtctttttta
    16201 gatgagcata caacggatag aggactctat gatgcgatga gttattcagt gaaagcaggc
    16261 gggaaaagaa tacgtccctt attactttta acggccgttg cttcctttga tgagcctatt
    16321 gatgttcccg tctatcaagt agcagctgct ttagaaatgg tgcatacgta ttccttgatc
    16381 cacgatgatt tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc taccaatcat
    16441 aaagtattcg gcgaagcatt agctatattg gccggagatg gtttgcttac aggagcattc
    16501 cagctaatca gtatggcaca cttaggtaat tctcctaagc ttctgctgct tcagcaatta
    16561 gctgtttgtg cgggatcaca aggtatggtt gctgggcaag ctgcagatat cgagggcgaa
    16621 tcgaaaaagc tttctttaga agaattagca tttattcatg aaagaaagac agggcatttg
    16681 attcgttatg ccttgctggc tggtggcata ttagcgaagc aacctgaaga aatcttattg
    16741 ctacttcaac gattagccga acatttaggt ttagcttttc agataagaga tgatctactc
    16801 gatgtgattg gtacaacgaa aacattaggg aaaacagctg gtaaagatga acgtatggaa
    16861 aaaaatactt acccgcgttt attaggccta gaaaagacac gtgaggcgtt ggagattgag
    16921 ctgttatccg ccaacaagat catagacaaa ttagaagagg acgttttttc ttttgatggc
    16981 gggctgatgc gccagatgat caagcaattt gatgtggaga aatgagggaa agaatggaaa
    17041 aagaacgcgt agatgtatta gcggtcaaac aaggattatt tgaaacaaga gaacaagcca
    17101 aacgttcagt gatggctggt ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg
    17161 gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg taaaaaactt ccttatgttt
    17221 ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa
    17281 aaacattgtt agatattggt gcatccactg gcggatttac tgatgcagct ttgcaaaatg
    17341 gcgcaaaaat gagttatgct ttagacgttg gctacaatca gctagcttgg aaaataagac
    17401 aagatcctag agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa cccgaagact
    17461 ttaccttagg aattccagaa gttgctgtta tcgatgtttc ttttatttca ctaaaactga
    17521 tgctgcctcc attacatgct attttaaaag aagagggaga agtgattgct ttaatcaaac
    17581 cccaattcga agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga
    17641 cccatcaaaa agttttagaa gacattttgt catttgcggc acaaggtgga tatgatgtgc
    17701 tggaattaag ttattctccg atcacggggg gagaaggaaa tatcgagttc ttggctcact
    17761 tgaggaaagt acccgaaagc ggaacaatca attcagctat taatatggca gaagtggttt
    17821 caaatgcaca cgaacagttt gaccataaat aaattgtaac tgtgaaaatc aagtgagaag
    17881 tatgcggatt cttctttatt ataccaagga aataagctat cattgattaa gaagaatatg
    17941 ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc gtttgattac tcgattgttg
    18001 agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata
    18061 tctgttactc aagctacgat ttccagagat ataaaggaac tgaaactgat caaggtaccg
    18121 gcttcagatg gcggctaccg ttatagcata ccagctgaaa caaacgaaga tgtaaatact
    18181 aaattggaaa aactattaaa agacgctttc gtggcagttg accaaatgga aaaatttgtt
    18241 attttgaaaa cattgccggg aaatgcttcg gcagcagcaa atttgattga taaacgttac
    18301 aagaaagaat tattttcgat tatcaatgat gacgataatg ttttgatgat tacacgaact
    18361 gaagaccagg cagttttatt gaagaaagac ttcctgcatt acctttaagt tgaggagcga
    18421 atgtgatgct acaagaaatc agtattacta actttgcgat tattccggaa ttgcggcttt
    18481 cttttcacga aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa tccattatta
    18541 tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag cgattatata cgtcaaggag
    18601 cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa acaagaagga ttctctgaac
    18661 tgatggttga attagggatc gaaacagatg aggacaatct gattgttcga cgggatatgt
    18721 ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat tatcacatta gctaatctga
    18781 gaagaatcgg cagttattta gtagatatcc aaggacaaaa cgaacatcag gaactgctcc
    18841 agcctgaatc acatcttgct ttattggatc gttttggaga tgtagctttc caacagaaga
    18901 aaaagagtta tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag
    18961 tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat gctccatttc cagcaagaag
    19021 aaatagctgc tgctgatttg caagtcggag aagaagagaa attaaaagaa gaaagagaaa
    19081 aacttagcaa ttatcaaaaa attgctgatg gattagctgc aggttacgga gcactgacga
    19141 ataatgaaca aaacagtgta gatggtgttg gcttggccgt ttcagaactg caagggatcg
    19201 cccatctaga tgtggagtat gaagcaattt atgaaaatat ccaaagtgct tactatttgc
    19261 tgcaagatgc aatcggcgat atgagtcgtc agattgattt attggagtta gatgagagtc
    19321 gtttagaaga agttacgcaa cgtttagaat taatccgaca gttgaaacgt aaatacggcg
    19381 aatccattga gtcaatattg gcttactatg acgaaatcac cgaagagcta gcttcttctg
    19441 atttttcaga aggtcaattg gacaaaatga aagaggaact agagcaaaaa gagcttctac
    19501 ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa
    19561 aatccattct gcatgaattg aaaagtctgt atatggaaaa cactgagttt gaggtacgtt
    19621 tcttaaaaga agaaaataga caattgaatc atgatggatt tgaccaaatc gaattttaca
    19681 ttacgaccaa tccaggagag ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac
    19741 tgtctcggat gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta
    19801 ttatttttga tgaagtggat acaggtgtga gcggaagagt tgcacaggcc atcgcagata
    19861 aaatttctca aatatcgaaa tactctcaag ttttgtgcat cactcatctg cctcaagttg
    19921 ctgcagtcgc cgactatcaa tattatattg tgaaagcagt gattgatgga cgtacgcaaa
    19981 cttctgtttc agagttgaaa acaaaagaaa gagaagaaga aattgcacgt atgctggctg
    20041 gaagtgagat tacaaaactc acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa
    20101 ggacctgagc cacctcgcag tggttcaggt ccttttcagc agctgatagc tatattttat
    20161 ttttacatga cactcattac agcagtcaat acaacagatc caatcgttaa aatcagcatt
    20221 gtccaaatga caaattttgt tactttagca aaagtacttg tttgtttttt ttcacgcatt
    20281 gttttcaacc tcgtcattaa ttagttgatt caagtgtaca atcttttttc catttccaca
    20341 agagggcttt tttataaaaa cttccctttt tttaaataat aggtagttac gatacaaata
    20401 aataatgtta atagaactag ccatataaag gcatcatccc tttcagcaat aggtaactta
    20461 atgttcattc catagatact gccgacgatt gtcggtatag ttaaaataat cgttaaagaa
    20521 gttaaaattt tcataatatt attcagatta ttcgatacga ttgctgaaaa agtctgattc
    20581 atttgttcta ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc
    20641 aatacatcac gtaaatgatt tttcaaagca tgattttctt ggaaaagttt attttgcgct
    20701 agttctttca atgcatctaa attagcagaa gtcgcttctc tgaaataaac gaggcttttt
    20761 tggatatcca tgatttgata aagttgtttg ttttcagtgg atacttgaag tttggcttcc
    20821 aaagtatcgc attgagattg aagcgtagct aaataactgt tgtaagagag tgtgacctgc
    20881 caaatgaatt gaaagaagat attgagatta ggcgaaatat cactttttgg aaaggtctgg
    20941 ctaaagactt tctttaaaaa cagtggttca tgattcgtaa ttgtgataag cttctttttg
    21001 ggcgtcaaaa taatagagag cgggtatgta tccacttgaa aataaccgct aggacttgtt
    21061 ttagcaaaag gatattgtaa caataataac gctggctttg tcaactttga ttgattgaat
    21121 tcttctgtac gggaattttc tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt
    21181 tcaaattgat gtgtcagctg atgaatctct tcttgattag gacgttctac acaataccag
    21241 atcgtttggt cagtttcctt tccggtatta cgcagtttct cattatctaa tgtaaaatat
    21301 ttgatcatga ttttcctcct gttgcctgtt tcttttatta taacggatag ggaattggaa
    21361 tacatggaat gtgtttgtaa gatatagaat ataactgcat aaatttaaat aaaatgaaac
    21421 agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt ttacgttcat
    21481 gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg ttttgtttgg
    21541 ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa aagcccctga
    21601 tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata cagggacaat
    21661 ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta ttgctgtttt
    21721 ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga cttggcgttt
    21781 ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca tactttctta
    21841 cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt
    21901 tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag
    21961 aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt
    22021 tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac
    22081 ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg
    22141 gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta
    22201 aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg
    22261 ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag
    22321 gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag
    22381 atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg
    22441 gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat
    22501 gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac
    22561 actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct
    22621 gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta
    22681 tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt
    22741 gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcctca
    22801 gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat
    22861 ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt
    22921 agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat
    22981 cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa
    23041 acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca
    23101 agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc
    23161 aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga
    23221 cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact
    23281 tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct
    23341 tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc
    23401 agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt
    23461 acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt
    23521 cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt
    23581 tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag
    23641 ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa
    23701 tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta
    23761 ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa
    23821 ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc
    23881 taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat
    23941 tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta
    24001 cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa
    24061 gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa
    24121 ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa
    24181 agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg
    24241 cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct
    24301 atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag
    24361 gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt
    24421 cttctcattt tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac
    24481 taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc
    24541 aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg
    24601 acggacaaga tatctacacg actttagaca gcagactaca aagttattta gaaacactga
    24661 tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa
    24721 aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag
    24781 gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag
    24841 gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga
    24901 atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact
    24961 ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga
    25021 tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat
    25081 ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta cctacattaa
    25141 atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa
    25201 tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca
    25261 gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc
    25321 aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa
    25381 gcgaaaacta cggtagtgct tatggtgtat atagtgtacc tggatacaat gtatcggcaa
    25441 aaaacggaac agcccaaatc gcttctgata cagggggata tcaaacagga gatacagcct
    25501 acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc ttgtatctaa
    25561 caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca aatccattga
    25621 tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa acaaaaacag
    25681 aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc
    25741 aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta
    25801 ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact ggtggtgata
    25861 agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg gggaaaatcc
    25921 tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa cttgctccat
    25981 atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa gagaaatagg
    26041 agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag ttgtgcaatg
    26101 acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca aggtcaggcg
    26161 attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac aatgggcggc
    26221 ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg gcaaaaacag
    26281 ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt aatcggcttt
    26341 cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc gaagcagaaa
    26401 ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc tgaaggttat
    26461 ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta tggtattttc
    26521 gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg catcgatgga
    26581 ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc ttggcatcag
    26641 catcagtatg atgtattaat catttgctta agtgttttag gaggtttact gggtttcttc
    26701 gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt agcgttgggc
    26761 ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct tttgattggt
    26821 ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt taagcttaca
    26881 ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg cggatggtcg
    26941 gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt gatcacatta
    27001 tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg aaaataagaa
    27061 agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat tacatgaatt
    27121 gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg aagcacaaga
    27181 gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat tgttggatga
    27241 agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt tagtagcaaa
    27301 agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg aagtagcgga
    27361 atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa cgatgacagg
    27421 actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta atattgggta
    27481 tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga tggaattgtc
    27541 tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga ttaccaatat
    27601 ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa
    27661 ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc agtcagaact
    27721 acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt
    27781 ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa aaataatgga
    27841 aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag ctgctatttc
    27901 tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc attttttcca
    27961 tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat tttataatga
    28021 ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg aaacgtctaa
    28081 ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat taattccttc
    28141 attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc
    28201 tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac aagcagttcc
    28261 tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag cgaatgccag
    28321 ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg
    28381 attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt acaggcggag
    28441 gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag actcaagaac
    28501 cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg
    28561 atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa atttccatgc
    28621 ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa aaaatcctca
    28681 gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga gcggtagtgt
    28741 acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt gtaccaggtg
    28801 ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt aaagatgcgg
    28861 cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca caagaagtag
    28921 cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa aagaaaacag
    28981 tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta tcagtttttt
    29041 taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat agatattatg
    29101 aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag ccttatatca
    29161 aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct ggtgcaactt
    29221 cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc ccatatgtta
    29281 cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca gcaaaaatga
    29341 ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa atcatgggtg
    29401 atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc cctgatgctt
    29461 caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt
    29521 gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa acaatttgac
    29581 tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa accaagagcc
    29641 aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag aaaaaacggt
    29701 tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat
    29761 caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc tgcccaattt
    29821 aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca ctgtattggg
    29881 tataccgctg atttttttga tctattatgt ttctccttat agcaaattac aagcagttgc
    29941 agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa aattgtcatt
    30001 aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc gccttaaaaa
    30061 agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg aattcgattt
    30121 ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat actacccagt
    30181 gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa atctccctat
    30241 cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat acaatcagtt
    30301 atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag caagcaataa
    30361 aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata tcaataatct
    30421 tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa aaggtgtgat
    30481 cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa cggaagaatc
    30541 gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag
    30601 tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa acggatcatc
    30661 tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta caactccgtc
    30721 aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc
    30781 agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat attttaaaac
    30841 ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc attcatggca
    30901 aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct
    30961 gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc agaagggatt
    31021 aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg agcagtacga
    31081 caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac
    31141 atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc aaaggaaatc
    31201 accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat acctcctgaa
    31261 cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga gggaatcaaa
    31321 gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt tacaggacct
    31381 aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa
    31441 atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga aaaagatttt
    31501 ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat gcatgataag
    31561 caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa agatatctct
    31621 attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta cggggatgca
    31681 tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg tcagtcagaa
    31741 ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat ggaacagatt
    31801 ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc aggtggcgtc
    31861 gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca agaaatcttc
    31921 ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc ggtatttaca
    31981 aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca attggcaaaa
    32041 attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca
    32101 aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa
    32161 gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat ttttgattaa
    32221 ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca atgacggcgc
    32281 agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc gaatgattga
    32341 agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag cattaaaaaa
    32401 ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt taggtgctgg
    32461 ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc
    32521 tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg
    32581 tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt
    32641 gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc
    32701 tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt tattagaagt
    32761 tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg ttttacgaca
    32821 aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc ttgactttgc
    32881 tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg gtgtggctag
    32941 tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac
    33001 atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg atatgacatt
    33061 gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg atgtaaatat
    33121 cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc
    33181 aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta gacaagcaca
    33241 aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac caacttcttc
    33301 agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg ttcgtcctag
    33361 agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat tcaaccgcga
    33421 agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa
    33481 ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag caagaaattc
    33541 aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta gctgtaacaa
    33601 aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg aattgtgcag
    33661 aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt cctgaaataa
    33721 agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta aatgaaattg
    33781 attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag cggttagaga
    33841 aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa catggaattt
    33901 caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg
    33961 ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat aaagtgtttg
    34021 gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat gcaccatgta
    34081 gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa ggtgcaacat
    34141 ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa aatgccgctt
    34201 ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga gtacgaatac
    34261 caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag
    34321 aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa aacggcgact
    34381 tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt aattgaacta
    34441 catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa
    34501 cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta ttctgaagct
    34561 atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt tcatttagta
    34621 gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta tgcactagat
    34681 ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg gatggagatt
    34741 gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta agaggaggaa
    34801 ctacgattta tactattgca ctattacttc atttacttca acgggcagcc tatttctata
    34861 gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat caatcagggc
    34921 taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat cgtctgcctt
    34981 tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg aacttagccg
    35041 tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat tgattgaagc
    35101 aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca aatgtatacc
    35161 agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg attgaacagg
    35221 ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa tcctatattt
    35281 tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc ggatatgaac
    35341 aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta gaggaagatt
    35401 ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta agtcatggga
    35461 aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc gatatcatct
    35521 cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt gttgctgcac
    35581 aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac acagaaatga
    35641 ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg cgactagaca
    35701 gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg atcgaagctg
    35761 gtaaagtaaa agtgaactgg acagaaaata caagacctga ttttgtactt gaactattag
    35821 atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag gggaagacga
    35881 aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa aaggtggttt
    35941 actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa aaatgcgggg
    36001 atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact atgaggaatt
    36061 gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa aacttgagta
    36121 cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag atacggctga
    36181 taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa
    36241 agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa ctgatgcaga
    36301 agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga
    36361 aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct tgatgcttga
    36421 gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac cattctccag
    36481 ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc ttgacaatga
    36541 aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga cggcatttga
    36601 tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa
    36661 agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag acactgcatg
    36721 gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag cagcggctga
    36781 cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt gggtggtacc
    36841 acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa caaataaagg
    36901 agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa aattccctat
    36961 gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat
    37021 ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc atgatggacc
    37081 tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca gtaaagacat
    37141 tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag gatgggatac
    37201 acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta aagaaatgtc
    37261 catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag ataagcaacg
    37321 agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg ttacattaga
    37381 tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa aaggctatat
    37441 ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt tagcggaagc
    37501 tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca atgtcaaaga
    37561 cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa caccatggac
    37621 attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc
    37681 cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat cagaaatcgg
    37741 ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat atatgactgc
    37801 aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg ttactttaga
    37861 cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact atatcgctgg
    37921 tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt ttacagaaga
    37981 agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca ctgatctatt
    38041 aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc ctcacgattg
    38101 gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat ctatcgataa
    38161 attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc catggggtaa
    38221 aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc
    38281 atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca tcacaccaga
    38341 aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct ggtttgaaaa
    38401 agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta atggcgaatt
    38461 tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc atgaagctgt
    38521 tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag gatcagacca
    38581 atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg gtgtagcacc
    38641 atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta agatgagtaa
    38701 atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg cagatatcct
    38761 tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta tggatatctt
    38821 aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct tattagcgaa
    38881 tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac gttctgtaga
    38941 caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag aagggtacga
    39001 caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg ttgatctatc
    39061 gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag atgattatca
    39121 acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa aattattgac
    39181 tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag aagaggagta
    39241 cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag aattgatgga
    39301 tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg aagaagcaag
    39361 aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc caaaacaaca
    39421 agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga tcgtttcacc
    39481 agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta tggtatttga
    39541 tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt
    39601 tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat
    39661 cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa
    39721 agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt aattttccac
    39781 gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt tcaagtagtt
    39841 cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc ggttgtgttt
    39901 ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact tcttcccaat
    39961 gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg taagcttctg
    40021 ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt tccgtttcaa
    40081 atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt ggctgaatat
    40141 agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt
    40201 tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct ttttcagtca
    40261 aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta tcgatagtga
    40321 atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct tcttcgcgat
    40381 agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga acgaagtttt
    40441 ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc ttttctgtac
    40501 ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct acaacctgta
    40561 aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag cccccgcgat
    40621 cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag cgattgttcc
    40681 attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg atcatttctt
    40741 ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct ctgatttgct
    40801 ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg atcaaacgat
    40861 tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc gttccaaaaa
    40921 attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag tgtccatcta
    40981 atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca tcatgggatt
    41041 gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc aatgcacgga
    41101 ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga gcggttccga
    41161 ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca
    41221 atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca tttttttcat
    41281 tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa
    41341 gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat aagctgcgat
    41401 gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt aatccaacaa
    41461 ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat aggactccaa
    41521 tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc ctccatgagg
    41581 acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt ccgaagtcac
    41641 atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta aatgttcaac
    41701 taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt gcagtccgct
    41761 tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt tcacaagttt
    41821 agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga tttgtttatt
    41881 gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat cttctcgatt
    41941 tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa
    42001 aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac gtttttacaa
    42061 caaaaaacga aagatgatag agaagactca ttctttcgtt tataaaggat atatatatac
    42121 attgggaggg atctattttt cagataacaa cttatatgaa gtgtctgcag attcgtcata
    42181 aagagtttgt tcgggataag aaaagataag ataaaaacca tactgatcac gtacgactaa
    42241 acgatattca tctatttcta ccacagatgt gccaagagac tgaccgttga tcaaaaacgt
    42301 gtaatcagat gtaatctgta attgttgttt ttgctggcta tcactaaaga accatgtacc
    42361 ttgatacttt gagagtcctt ctgacttttc cgcttgtttt ttctttttca agtaaataaa
    42421 acttgagaga acggtaatga gtgaaccaag aaaatagatc atttttttta tcaccaaatc
    42481 actcctttat gagaaaataa tcgcatagac atattctttc ttctttattt cattgtaccg
    42541 aataaaagct aaaatttctc taagactttc gactggtttt tttactgttt ttctacaaat
    42601 tatgatagga tacatagtag gaagaaaggt gacataattt gaagtatcag caagtggaaa
    42661 tagctttttt tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag
    42721 aaataattat tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg ataccaggcg
    42781 ctgaagtggc tattgtattt gctcctggac ctaaacggaa aaccgcctat gatctgattg
    42841 ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca attgcctaat cggctggcaa
    42901 ttgatggtat tttagacggc acgatccttt taccaggatt aactggtcca tttacggtat
    42961 acaagcgtga agtaacttat cttcactcca aatttgatat ttatttagag acggtggtcg
    43021 gacaaaaagc atttgttgaa gtcaaaggta tgacacttga aaataaagcg attggtgctt
    43081 ttcctgatgc gcccacactc agaggattga aacatatagg agaattaacg tatgccgcac
    43141 aagatggtta tgccgcttat gtcttgttta ttgctcagtt cgaacatctt catctggcaa
    43201 cgatccatga agaaatgcaa ccagcactag ctgatatggt tcgacatgcg cagcaaagcg
    43261 gagttcaaat cctcgcctat aattgtcaag tatcggcaga tgaggtaact gtcaagcagg
    43321 ccattccatt tgatgtagat atcccttttg aagatcctat acaatagaaa gaaggtaaaa
    43381 taatgatcaa cttaggtatt atcggaacaa attggattac tcatcaattt gtccaagcag
    43441 ctttaagcac aaaaaaatat gaattgacag cggtatattc tagacatttg gaaaaagcac
    43501 aggaatttgg ttctcattat gaaggggata tcgaattcgc aacagatttg aagacttttt
    43561 tcaatattac tcatatggac accatttata ttgcttctcc gaatagtctt cactttagtc
    43621 aagctaagca agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga
    43681 cgccaaaaga aatggatgaa atcatccatt tggcaaatga acacaatgtg tatttttttg
    43741 aagcagcaag aaatatccat gaatcaggtt ttaaaaagat tgccgattac ttaccattaa
    43801 aagacgagat aatcggtgca aatttcagct atatgaaata ttcttcgcgc tatgaccaag
    43861 tattagatgg agaagaaccg aatatcttct cgactcattt ctcaggtggt gcattgatgg
    43921 atttaggtgt ttaccttgta tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc
    43981 attatttccc aagaaagatt tctactggtg tagatggtct tggatggggg atcttacgtt
    44041 atgagaattt tgatgtatcg atccagccag gtaaaatcgg tgattcttat ttaccgtcag
    44101 agatttattt tgactcagga acgctagtga tgaatggagt caatgcaatt gaacaagcac
    44161 aatttcatga tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct gcagaaaatc
    44221 caatggcaga tgaagcagag gattttgcca atgtcatggc tcatccgaaa gatccagctt
    44281 ggggattgtt gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg
    44341 atttaagaaa aaatggcggc atcaaattcg atgcagataa tgaggaagat ttttaatgaa
    44401 cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa tatgaggata aagggtttat
    44461 ctcaccttct cttattcaac gagcagtttt tcagccacta agtaataaac aaagtatagt
    44521 agcggtttct cctacaggtt caggtaaaac actggcttat ctttggcctc tactactaaa
    44581 cgtggaacca ggtgaagcaa gtgcgttggt catttttgct tcttctcaag aactagctat
    44641 tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt
    44701 ggtgggcgga gccaacgtca aacgtcagat tgagggtttg aaggaaaaac cagaaatctt
    44761 gattggtaca ccaggaagaa tattggaatt gatgaaagca aaaaaaataa aagcacatca
    44821 agtgaaaaca atggtatttg atgaagcaga ccagctattt gacgctggga acagccaaat
    44881 cattgatcaa attcttcatc aagctccaac agagtatcag ctagcttttt tttcagcgac
    44941 agcagatcgt tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg agacaattga
    45001 cgtgacagca gaggacgatt ctcgaaaagg actgagacat tactttttac gtgtaccgat
    45061 acgaaagaaa gatgactact tgagacgtct cacgcatata gaagattttc atggtctgat
    45121 tttctttaat caactgactg aattaggcgt aatggaagaa aaattgctgt atagaggtgt
    45181 accggtagga agtctggcct ctgatcagaa caagctactc cgaaaagcag cgctagaaca
    45241 attcaaaaag aaaatggtca gagcactttt gaccacagat gtggcggcta gaggtttaga
    45301 tattacaggt cttccttatg tagtgaatgc agaagttccg ttaagtgaag aagcttattt
    45361 gcatcgttcg ggaagaactg gtcgaatggg taatgaagga atagtaatta cactcgtaca
    45421 agataataat ctaagagatt taaaacggct tttacgtcca tcaaatataa cacttgaaga
    45481 gattttttta tacggaggta agctgcaaac tgagcagcca accaaagaag atgcagctcc
    45541 tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc cagaatataa
    45601 caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc
    45661 aaggcgcaaa taagcgcctt tttatgctat gattatgaaa acgggaacaa aattggttgc
    45721 cagaggaggg gttatgtgaa agaacgtcaa gaagagatct tgcagctatt gacagaaaac
    45781 caacaaggat tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca
    45841 agccgatatt taagtgaact atacaaggcc catcatattg taaaaaccgc tggccgacca
    45901 gtcgtttact ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt cgactcttct
    45961 acgcaagtga cttttgaaac actcgttgga gaaaatgatt ctttaaaagt cagcatacag
    46021 caggcaaaag ctgcgattct ttatccaccg cgggggttgc atacgattat ttttggagaa
    46081 actggtacag ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat cgattcagag
    46141 atgctttctg cagatgcgcc atttgtttct ttcaattgtg cggattatga tcaaaatccg
    46201 caactgctct ttggacacat ctttgggatc aaaaaagggg cttacacagg agcagctcaa
    46261 gatagtcccg gtctaatagc caaagcagac ggcggtatat tgtttttaga tgagatccac
    46321 cgtttaccgc cggaagggca ggaaatgctg tttactttca ttgataaagg tatttaccgt
    46381 ccgcttggag aaagtgcaca agtacatgaa gcatctgttc agatcattgg ggcgacgact
    46441 gaatcttcag aaagtttctt atctacgttc aatcgacgga ttcctatggc aatcaccttg
    46501 ccgaatcttg cttcacgttc actggatgag cgttatgaaa tcatctctct ttttatcaaa
    46561 caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc
    46621 atgctttatg atgcggaagg aaatatcggt caagtgaaac gagacttgaa actagtctgt
    46681 gcaaaatctt ttttacatta ccgtactcat catgagaaaa agctaatcat tcgaaaagaa
    46741 gaactttccc ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc
    46801 gaccgattca tggacagcaa aagtcagtat ttgacatttg aaccaggatt tgctgatgtt
    46861 gtctggtcac aagatccaga acgaaacatg caggtctata acgatattga ggaaaaagtc
    46921 acttctcttt ccgaaaccgg tgtggagaac attgatctag agaccctcat ttcaaaagat
    46981 gtggatgctt atttccagac atatgtcaaa gaattgacta aaagttcgat tcccaaagat
    47041 cttttgccag atgaaatctg gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg
    47101 ttagaccgta tttataatga aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca
    47161 ttggatcgtg tgagagaagg tcatatgatc gtccacccag atttgaataa cgtacggaaa
    47221 aattttaaga gggaattcca ggtagcaatc gatctttcaa cgatcattga agaagaagaa
    47281 caagttgaga ttccttttga tgaaatcggc tttattagta tgtttctttc tatcaatcta
    47341 ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc tcatattgat gcacggcaga
    47401 cagactgctt caagcatgtt ggagatggca caagaactat taggaacaaa agtagggaaa
    47461 gcttttaata tgccactggt tatggaagtt caaacaatgt atgaagaagt gttacaatat
    47521 gtcaaaaact atcaagagca tttgacgaat ggcctactct tgttaacaga tatgggatca
    47581 ttaaacacct tcagtgattt gatttatgaa gagactggca ttcggacgaa agctatcacg
    47641 atgacaagta ccatgattgt gatagaagcg atacggatgg caagtgttgg aagaagctta
    47701 gaagatattt atcaaaatat ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa
    47761 gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt
    47821 gaaggagtag ctgccaaatt atatcagcgt attatgccag tgattaatca aaataaggta
    47881 gaactgatcc agatgcagtt tttagaacgt gaatcattca aaaaacacat cgatgcctta
    47941 ttggaagaat atgagatcaa agcaattgcg ggaacagttg atgttgatta tcaaaatatc
    48001 ccattttttt cagcctatga cgtttttgat gatgaaaaac taaatatttt aaagcgtata
    48061 gctgatgatg agatcccgac agctaaaatt gtcgattcac ttgctggatc attaagcgat
    48121 gtcttttcta ttgatgaact gatccgttca ttgcaaaaga cagttcagca gatccagacc
    48181 gatttgtcga ttatcgtgga acctggagta gatactggaa tcgttatcca tttagccttc
    48241 atgttagacg gattgatcaa acaagaaagc catcggcaat ttgagaaact gtctattttc
    48301 tcaaaaattt atcgtttgga aatggatatc gttcgggcaa acttgatggc actagaacga
    48361 aaatatcagg taaaaattcc agaagatgat atcgcatttc ttacacaaat gtttcttgaa
    48421 aataaaataa aaaaatgaac acactaatca tactgtttac acactaaaca tctgtgtgcg
    48481 cactataaaa aagagctaaa aagcatgaaa acgctatttc agctcttttt tttattggca
    48541 tgcttcttgc gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa
    48601 aacaatcatg ttagtttgtt cagcagggat gagtacgagt ttattggtca caaaaatgca
    48661 aaaagcagca gaagaaaaag ggatggaagc cgatattttt gcggtctctg cctcagatgc
    48721 agataataat ttagaatcta agaatgtcga tgtattatta ttaggtcctc aagtacgttt
    48781 catgaaagct caatttgaac aaaaacttgc gcctaaaggt attccattag atgtcatcaa
    48841 tatgcaagac tatggcatga tgaacggaga aaaagttctt gcacaagcag aaaatctaat
    48901 gaaataaatc aagtatgcag ttgggggtat aacaacgtgg aagatcaaca aaatctagaa
    48961 gccatcatgg ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt
    49021 gcggctgcta aaatcggtga ttttgagtta gcggatagga aaatcgctga tgcagaagaa
    49081 tcacttatac aagcgcatca tgcacaaaca gacatgttga cacaagaagc acaaggaaat
    49141 catatgcaag tgacattatt gactgttcac agtcaagatc acttaatgac atctattgcg
    49201 tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag cgaataaata
    49261 caacttcttt tttgaacgga agaagaaaaa tttttgaacg gattttttat tttgttataa
    49321 ggagaactct ctttatataa aaaacttata ggaggaagta aaaaatgaac ggactatcaa
    49381 gttggatgga gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag
    49441 ttgctttgcg tgatgctttt atcggcatac tgcccgcaac aatggcggga tcagtcgctg
    49501 taatgatcaa tgcaattatt cgtgatttac cgcaacagtt taacagtacc tatgctgaca
    49561 gtttagcagc agacaaagga atctttgccg tattgaatgt catcatcgga atcaatggtt
    49621 ttgtctggaa cggtacacta gcaatcgccg gattgatctt tgccttttct tgggggtata
    49681 atcttgcccg agcttatgga gtaaatgatc tagctggggg gatcgttggt ttagctacct
    49741 tgattcaagg tatttccttt ggtccagcat tatcagcagc attagatgtg aatgttcctt
    49801 caaatgtcgc agatgcaatc aatagttctg agattggtgc aacgattgca gatggcacac
    49861 tgtcagttgg attatggggt tggctaaaac tagatcattt gaatggaaac gcttatttta
    49921 cagttatgat catgggagct atttcagtca ttattttttg taaattgatg cttgccaaca
    49981 ttacgatcaa aatgcctgat tcagtaccac cagcagtatc taaagcattt gcagccattt
    50041 taccagcgac gatcgcttta tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag
    50101 ctgatgggca actgatcatt gatttggtgc aaaaatatat tgcagaacca ttcctaggat
    50161 tatctcaagg gttgggagca gtattgatcg tcacagtatt tgttcaagta ttctggttct
    50221 ttggtattca tggatcaaat gtattggcac cagtgctaga gggaatctgg ggacaagcac
    50281 aactaatcaa tattgatatt ttccaaaaag gttataacgg aaaaactggt acaccagccg
    50341 tgttagatgc aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt
    50401 ttgcatggtt tggcggatca ggcggaacga tcgtcttact gatagctatc ttgattttct
    50461 ctaaacgtgc agattatcta acagtagcta aattatccat tgggcctggt atcttcaata
    50521 tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat
    50581 tcttgattgc tccagtagta gcaacagcaa ttggatggac agctacttat cttggcctag
    50641 tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc gttcttattg tcattcttag
    50701 caacaggagc ggattggcgg gcaccgatcg taacattagt atgtatggtc gtgactttct
    50761 tgatttgggc accatttgtc atctctgcaa acaaaatgga ccctgccgca ggcgaacaac
    50821 aataagatga acgtgaggag cgggacgctt taagtttcgt tcctcataat gaattttcga
    50881 ggaggaactg aaatgattac aattgccagt gcaatgcaac aagatgaaat caaagaattg
    50941 ttagaaggat acaatgaaga aggaatgact tttacattta aagaaaaaca aggaatcaag
    51001 ctgttctttg aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta
    51061 atcaaagcac agccttgggg aacagtcctt tattttcaat caacagctgt ttagaaaatg
    51121 gtttaaaaag ataggatggt tgagatgata aaaaacggtt tatttttatg tagtattggt
    51181 tctttgatcg tgttgtatag tttgaatcca cattcgcaaa ggtatgattt gtatagtatg
    51241 gtcacaggtc tttttctaac aggattagga atctatttct tcatgaaggg gaaaaaaaga
    51301 gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa aaaattaaat attccagcat
    51361 cgttttttta cgataaagtt atggactctg tccttttcga tgtacgtaaa gcgaccggca
    51421 aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata
    51481 accgggctcg cataaaaatc gaagaagtca ttgaaaacca gtcttataag tttcgtacat
    51541 cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa accattggac gattcgacct
    51601 gtgaggttca ttacacagaa aaaatggagt cttatggcat gcttcaaaag ttgaatgata
    51661 tgcttttagg aacaatcttg atgtatttta aacggagacg tttcaaaaaa atgctagata
    51721 tgatcgaagc aacgtattaa atcaatgaaa catggtacta tttagttatt gaaaaatgaa
    51781 gtaagctaga aggaattttt cataggaaga tagatctttc ctgcgaatcc tcactagctt
    51841 ttttgtagga aggggcagca aaatgacaaa caaagtaaga gagctaggtg tatcttcgat
    51901 tgaggaaatg tttgatttag ccacttatgc atttaatggg gcagatacac ctgaaaggcg
    51961 agaacgtttt cgtacacttg cggagaattc ttggaattac ggattttttg atgaggagga
    52021 tcggctaaca agtcaagtca tggcgacccc ttttccagtg aatttttacg gacaagagta
    52081 cctaatggcc ggcattggct atgttgcttc ttatccagaa gcaaggggac aaggcgggat
    52141 caatcagatc atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta
    52201 tctggcgcca ttctcctatc ccttctatcg gcgctatggc tatgaacaat catttgataa
    52261 aatcagttat caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat
    52321 aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga agaatttatg aacagatgcc
    52381 ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg tatacgtata aattcaagca
    52441 aaaaggcaat taccagtttg ctttgcattt tgatgataat gaggaagcaa cgggatatat
    52501 cgtttatcag cttgcaacgt cttcctttat tattgcggag tggggatttt tggatcatga
    52561 tgctttttgc agccttgttc gcttcgtaag ttctcacaat ggtgcttttg aaacatttga
    52621 atattcatgc ggctacggcg gtaatgatca aaattatcta ttagaaaatc catttgcttc
    52681 tgtaatgatc acaccttata tgatggcgag gatcgtagat atttctttgt ttttggaaaa
    52741 ttatccattt aaaaagagac aagctgcatt tgccctacaa atagaagaag atcattatgc
    52801 gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa
    52861 agtagaacaa acaaaactcc caatcgttcg aggaagtatc caacatatca cgcagctatt
    52921 attagggtat cgtaaagtgg aagagttggt attccaagaa agaatacagg tgacaaaaga
    52981 atatgaagaa acgttaagct caatcttgcc acagcaacag ccaattctta atgactattt
    53041 ttgataaaaa acgggtgatg acaatatttt tgtcaccacc cttttgtttt tcatttggca
    53101 tttgaaggaa aatgttaaag taaatcctct tttttcgtaa atttgaattt tttgttttct
    53161 tttgttttag ggttttcttt tttgtggtct tggattgctt ctttcgtaat cttttcccga
    53221 ttttttttag atgaatctaa gcgttctgcg tatacttttt cttttggctt atcattcatt
    53281 ggaaaggacc tccctacatt atttcattct acctctagtt tatcatctgt taaggactag
    53341 tggaaacgtt ttgcctaact tataagctga tttttttagc agataagctt gcaagtaaaa
    53401 aacaaacaag ctacaattga tttattgaaa ttgttcctgt agctcagtag gatagagcaa
    53461 tcgcctccta agcgataggt cgctggttca attccagtca ggaacgtata aataacccct
    53521 gtatcctttg cggtacaggg gtttgttttg tatttgtgcc aaaaatgtgc caaactagtt
    53581 atcattttta tataaactat cgaacacagt attgattttt tctttatcct gatcttcaag
    53641 ctctcgaaca atatgaa
//
LOCUS       DAZKNM010000002        52037 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000002 DAZKNM010000000
VERSION     DAZKNM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.002"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..258
                     /locus_tag="ACG8M9_000059"
     CDS             <1..258
                     /locus_tag="ACG8M9_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HFQ0376867.1"
                     /translation="IMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQ
                     IAKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            495..1037
                     /locus_tag="ACG8M9_000060"
     CDS             495..1037
                     /locus_tag="ACG8M9_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0376868.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1120..1671)
                     /gene="lepB"
                     /locus_tag="ACG8M9_000061"
     CDS             complement(1120..1671)
                     /gene="lepB"
                     /locus_tag="ACG8M9_000061"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0376869.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1823..2050)
                     /locus_tag="ACG8M9_000062"
     CDS             complement(1823..2050)
                     /locus_tag="ACG8M9_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0376870.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2047..2565)
                     /gene="msrA"
                     /locus_tag="ACG8M9_000063"
     CDS             complement(2047..2565)
                     /gene="msrA"
                     /locus_tag="ACG8M9_000063"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0376871.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2585..3298)
                     /locus_tag="ACG8M9_000064"
     CDS             complement(2585..3298)
                     /locus_tag="ACG8M9_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0376872.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3301..4197)
                     /locus_tag="ACG8M9_000065"
     CDS             complement(3301..4197)
                     /locus_tag="ACG8M9_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0376873.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4355..5197)
                     /locus_tag="ACG8M9_000066"
     CDS             complement(4355..5197)
                     /locus_tag="ACG8M9_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0376874.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5329..5982
                     /locus_tag="ACG8M9_000067"
     CDS             5329..5982
                     /locus_tag="ACG8M9_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0376875.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6045..6572)
                     /locus_tag="ACG8M9_000068"
     CDS             complement(6045..6572)
                     /locus_tag="ACG8M9_000068"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0376876.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6592..7539)
                     /locus_tag="ACG8M9_000069"
     CDS             complement(6592..7539)
                     /locus_tag="ACG8M9_000069"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0376877.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7551..9434)
                     /locus_tag="ACG8M9_000070"
     CDS             complement(7551..9434)
                     /locus_tag="ACG8M9_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0376878.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(9597..11675)
                     /locus_tag="ACG8M9_000071"
     CDS             complement(9597..11675)
                     /locus_tag="ACG8M9_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0376879.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11688..11963)
                     /locus_tag="ACG8M9_000072"
     CDS             complement(11688..11963)
                     /locus_tag="ACG8M9_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376880.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12325..12780)
                     /locus_tag="ACG8M9_000073"
     CDS             complement(12325..12780)
                     /locus_tag="ACG8M9_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376881.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12934..14142)
                     /locus_tag="ACG8M9_000074"
     CDS             complement(12934..14142)
                     /locus_tag="ACG8M9_000074"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0376882.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14254..14586)
                     /locus_tag="ACG8M9_000075"
     CDS             complement(14254..14586)
                     /locus_tag="ACG8M9_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0376883.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14734..15609
                     /locus_tag="ACG8M9_000076"
     CDS             14734..15609
                     /locus_tag="ACG8M9_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0376884.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15691..16272)
                     /locus_tag="ACG8M9_000077"
     CDS             complement(15691..16272)
                     /locus_tag="ACG8M9_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0376885.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16278..17537)
                     /locus_tag="ACG8M9_000078"
     CDS             complement(16278..17537)
                     /locus_tag="ACG8M9_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0376886.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17539..18585)
                     /locus_tag="ACG8M9_000079"
     CDS             complement(17539..18585)
                     /locus_tag="ACG8M9_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376887.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18820..19095)
                     /locus_tag="ACG8M9_000080"
     CDS             complement(18820..19095)
                     /locus_tag="ACG8M9_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0376888.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19338..20648)
                     /gene="der"
                     /locus_tag="ACG8M9_000081"
     CDS             complement(19338..20648)
                     /gene="der"
                     /locus_tag="ACG8M9_000081"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0376889.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20830..22059)
                     /gene="rpsA"
                     /locus_tag="ACG8M9_000082"
     CDS             complement(20830..22059)
                     /gene="rpsA"
                     /locus_tag="ACG8M9_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0376890.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22194..22874)
                     /gene="cmk"
                     /locus_tag="ACG8M9_000083"
     CDS             complement(22194..22874)
                     /gene="cmk"
                     /locus_tag="ACG8M9_000083"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0376891.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(22923..23558)
                     /locus_tag="ACG8M9_000084"
     CDS             complement(22923..23558)
                     /locus_tag="ACG8M9_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0376892.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23624..25027)
                     /locus_tag="ACG8M9_000085"
     CDS             complement(23624..25027)
                     /locus_tag="ACG8M9_000085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0376893.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(25014..26045)
                     /locus_tag="ACG8M9_000086"
     CDS             complement(25014..26045)
                     /locus_tag="ACG8M9_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0376894.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26076..26297
                     /locus_tag="ACG8M9_000087"
     CDS             26076..26297
                     /locus_tag="ACG8M9_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0376895.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26462..27199)
                     /locus_tag="ACG8M9_000088"
     CDS             complement(26462..27199)
                     /locus_tag="ACG8M9_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0376896.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27352..28692)
                     /locus_tag="ACG8M9_000089"
     CDS             complement(27352..28692)
                     /locus_tag="ACG8M9_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0376897.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(28929..30452)
                     /locus_tag="ACG8M9_000090"
     CDS             complement(28929..30452)
                     /locus_tag="ACG8M9_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0376898.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(30830..31441)
                     /locus_tag="ACG8M9_000091"
     CDS             complement(30830..31441)
                     /locus_tag="ACG8M9_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0376899.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31564..31689)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31771..32487)
                     /locus_tag="ACG8M9_000092"
     CDS             complement(31771..32487)
                     /locus_tag="ACG8M9_000092"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0376900.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32493..33062)
                     /gene="scpB"
                     /locus_tag="ACG8M9_000093"
     CDS             complement(32493..33062)
                     /gene="scpB"
                     /locus_tag="ACG8M9_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0376901.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33046..33816)
                     /locus_tag="ACG8M9_000094"
     CDS             complement(33046..33816)
                     /locus_tag="ACG8M9_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0376902.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(33813..34184)
                     /locus_tag="ACG8M9_000095"
     CDS             complement(33813..34184)
                     /locus_tag="ACG8M9_000095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0376903.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34203..35090)
                     /gene="xerD"
                     /locus_tag="ACG8M9_000096"
     CDS             complement(34203..35090)
                     /gene="xerD"
                     /locus_tag="ACG8M9_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0376904.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35186..35659)
                     /locus_tag="ACG8M9_000097"
     CDS             complement(35186..35659)
                     /locus_tag="ACG8M9_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0376905.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(35764..36627)
                     /locus_tag="ACG8M9_000098"
     CDS             complement(35764..36627)
                     /locus_tag="ACG8M9_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0376906.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(36823..38922)
                     /locus_tag="ACG8M9_000099"
     CDS             complement(36823..38922)
                     /locus_tag="ACG8M9_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0376907.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(39142..40251)
                     /gene="rpoD"
                     /locus_tag="ACG8M9_000100"
     CDS             complement(39142..40251)
                     /gene="rpoD"
                     /locus_tag="ACG8M9_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0376908.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(40275..42116)
                     /gene="dnaG"
                     /locus_tag="ACG8M9_000101"
     CDS             complement(40275..42116)
                     /gene="dnaG"
                     /locus_tag="ACG8M9_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0376909.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(42298..44613)
                     /locus_tag="ACG8M9_000102"
     CDS             complement(42298..44613)
                     /locus_tag="ACG8M9_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0376910.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(44798..45658)
                     /locus_tag="ACG8M9_000103"
     CDS             complement(44798..45658)
                     /locus_tag="ACG8M9_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0376911.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(45717..46349)
                     /locus_tag="ACG8M9_000104"
                     /pseudo
     CDS             complement(45717..46349)
                     /locus_tag="ACG8M9_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            46516..47277
                     /locus_tag="ACG8M9_000105"
     CDS             46516..47277
                     /locus_tag="ACG8M9_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0376912.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            47303..49894
                     /gene="mprF"
                     /locus_tag="ACG8M9_000106"
     CDS             47303..49894
                     /gene="mprF"
                     /locus_tag="ACG8M9_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0376913.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(50082..51269)
                     /gene="tuf"
                     /locus_tag="ACG8M9_000107"
     CDS             complement(50082..51269)
                     /gene="tuf"
                     /locus_tag="ACG8M9_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0376914.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            51637..51993
                     /locus_tag="ACG8M9_000108"
     CDS             51637..51993
                     /locus_tag="ACG8M9_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0376915.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        14495 a        10872 c         8928 g        17742 t
ORIGIN      
        1 ataatgcttc tctctcatga tttttataaa aaatttataa aagataaaga aatagctgat
       61 aataaaaaaa caagtggtgc tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa
      121 aaatttgctc aaattgcaaa agaaaatggt ggtactttcc acgaagtaga tatgggaatg
      181 cctgaagaag aaatgttttc attagaagtc caagatctag acggaaatac attagaaccc
      241 gtatggatga agatgtaagc agataactaa tctgacgttt tttataacaa aaaaacaaag
      301 gcggatgata cgataagtat tctccgcctc tatttttttt attcgtacaa tctctttttt
      361 acagtttata aaagcagtct tctcaccctt tttttaattg ttagatgcca gtgtatgtaa
      421 atcaccaatg tgtcatgaca cattggtgat tttatggtat ccttttatta ttaactgaca
      481 aaggagagaa tattttgaac actttgattg caaaagacgt ttttagattg cccgaaatga
      541 atgcaagatt gttgtcgacc tctgctcttt tattagccct tgatgtcatc ttatacaaga
      601 tagcgattgg tcctgcttat tttcaaatca cattcggatt tttaagtatg gcgcttatgg
      661 gttatctata tggaccgatc tgggctggtt tgcttgaaat gttgtcgaac actttaaatt
      721 tcacgatatt tggttcagga acgtttcaag ctgcttttct tatcacggct ttccttggtg
      781 gatttattaa tggcttattt ttatatcata aaaagataaa cctgccttcc gtcatcgctg
      841 cccaattatt gattatgatc ccagtcagcc tttttatcaa ttcttggctg atgtctgttt
      901 tgtttggtgc agatttcaaa gtaatttttc taggtagatt catcaggaat atgatccaaa
      961 tccctattca aataatggtt gtttattcat ttatcaaaac tttagaggct agaaaagtgt
     1021 ttaaacgagc tgtttgattt tacgtactaa ggtagtaaac tgattttgga ttaaacaata
     1081 aagcgtgcag tctcaacaga ctgcacgctt tattgtttct cactggatcc attttatttc
     1141 atcaagtgga taatagacaa atcttgcttt acctagaata tcagttttat tgatcgtgcc
     1201 aaacgaacga ctatcttttg aaactcttcg attatctcca agtacaaaat aattatcttc
     1261 cggaagcttg gattgtcccg ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt
     1321 tgcatgatct gatttcaggt ttttagttag ataaggttcc ttaactactt tcccattgat
     1381 cttcaattga tcattttggt agctgacatt ttctcctgga agaccgatta ctcgtttgat
     1441 ataaatcgtg ccatctgcta attgaaaaac aacaatatca aatcgtctga tctccgaaaa
     1501 tttttccatc acaaccatat caccttgctt caacacgttt tccattgaat tgccttccac
     1561 tggtaccggg atgaataaaa atccccgcaa catgaacgca aggaatacag aaacaagtaa
     1621 gtatttgaat acaagccaga agcgattaat atagcgttgt ttttttgtca atctctatca
     1681 atccatccct ctttttttca ttctttcttt attataagaa aaatatcaaa aaaatgcgac
     1741 tagatagctg aaataaaaat aattctttca attaggctat tggacaaaaa acttttttga
     1801 tcataaagta caaaactatc tcttatatgt catgattatt ttctaaatat ttttcccatg
     1861 cttcgtcaaa gatatccatg gacggcaaat agtcgacatt cagttccaga taattagaaa
     1921 tttcttcgta gctagagctg tgcttaggaa attggatgtc tttaccggct tctgtagcga
     1981 aaacagataa agcatcatgt gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc
     2041 ttcgattcac ccttcgttcc tccaattttc ttcgataaag gcagcccggc gtgcatggtt
     2101 tcttttaaaa ttttcaggat gctttttata atactcttga tggtcttctt ctgctagata
     2161 aaatggttgg gctggttcga tttttgtaac gattggctgt tcgaaacgtc cactttcagc
     2221 taactgtttc ttactttttt cagcaatgat ccgctgttct tctgtcaaat aaaagatcac
     2281 cgggcgataa ttgtcaccgc gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg
     2341 ctgccagtaa agttctacta attgttcata tgaaatcaat gttggatcat agataatctc
     2401 cactgcttct gtatgtccag tcgtttggct ttttacttgt tcataagtcg gatctggtac
     2461 atgccctcca gtatatcctg acatcactgt atggatacca ggaagcgtgt cgaaaggctg
     2521 gatcatacac cagaagcagc ctcctgcaaa tattgctcgt tccattatga ttccccctct
     2581 aatactactc atccgatgat tttttatttt cttttgtctt gctttcagta ctgctggtgc
     2641 tagttttgtt ggtatttgag ttgtttgaag catcagaggt atcttttggc aaatatagac
     2701 taaaccgaat gtcatcattt actaagtcga ttttatctgc tttaatatac atcccgtttt
     2761 gcattcggaa ttgatccaac ctaagcatga tagtcagatc atctgtattg acttcgatcc
     2821 aatctggtaa tttatagttt ctcttgatca tattcatcac atctttgatt ggcaaactca
     2881 atgttccgat agaaagactt ctagctttta gttggacatt tccgttttcc atcacatacg
     2941 gatcaaaata taaatagaaa tcgataggaa aacctagtac ttcgaaagta ccattgagta
     3001 gtgcctcatt ttccaaataa aatttatatt caatatcact atctttttga tactcactaa
     3061 ggaaaaagct gatgatttga ttgacttttt ctttatttga attgatcgtt aagacaggat
     3121 ctccttgtct ttccgtaaga gctggtattt ctgggagttc tggttcccga ttagcaaaaa
     3181 tacggctgcc taaaaatacg gtcgaaccaa gtactacccc aagaaggata aggaaggcga
     3241 atttccagta atttgttttc tttgttttgt ttgaagatac gtcagatctt gttttcatgt
     3301 ttattgatca tcctccttct ctaaccattg gttttgtgtc tcgatcagct gatctctcac
     3361 tgcatttgcc atcaattgat aacctaaatt atttggatgg aaatgatctt cttcatagag
     3421 cacattattt ttgatgccat ttagattttc tgtcgtgcta gttgtttgat ctgttccttc
     3481 ttctgtgaca cctacttcct gattcaatcc ttgataaagc aaatcattga ttgggataaa
     3541 gtagcagttc ggcatttttt ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat
     3601 tgtctgcatg tcagtgatct caggaaagtt caaataaaat ggattgtaga tgcctaaaat
     3661 gtaaataggt gcggttttat tgagggatcg aaattcttcc aatagttctg tgacattttc
     3721 ctgatagttt ttcagtggct ttttaaagga attgaccgtt aacccgaata aattattttg
     3781 gataactttc atcaaatcat taccgccaac agtcagtgtg ataaaatcag ctgttttgat
     3841 attgtttcga atttcttctt ttgtttttac tcgctttaag atctggtcac ttcgttcacc
     3901 atttacacca tagttttctg tttcaaccgc ggtcaaatta taacggtctt ttagatcatt
     3961 tgcgacaagg ggaacgaatc ctccttgctt ggtttgatct cctactcctt cagtcagcga
     4021 atctccgata gctatgtagt gaagtttttc ttttttattt ggatcttctc tcacagtagt
     4081 tactggtttc attagtgggc cagctttcgg tatagaagca gctaaacctg catagatgat
     4141 gatcgcaatt aataatggga acaaaacgag gatcaaatgt cgtttcgtta atttcatttt
     4201 ttcacctctt gtattcgatt ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct
     4261 ttatgaaaaa agaaaacaat tattttccta attttataaa gaaaaagaga ataagctgca
     4321 agcctttcag gctgagggct tatcctcttt tcgactaatc tacataatac atgattgcaa
     4381 acgcgtcttt accagtatgc gtagcgataa ccggattcgt atgcagtaca gggatatgca
     4441 tgtccgggaa taattcttgc aaaccctctt tgaattcttg cgccaattct tttgcatccg
     4501 catgtgaaat accgatttgt cgcacatttg gcaattcttt cagttcattt ttcaaattat
     4561 taaaccattt atcgaacgtt ttctttcctc gccccttagt tacagggatt aattcagaat
     4621 gggcgaaatt catcaccacg cgtatgttca aaacactaga cagcaaacca gttgttcggc
     4681 tgattcttcc accttttacc aagttatcta gtgtggatat tccgatgtat aattttgtat
     4741 tgtcttgtac ttttttgact tcttcaagga ttttctctac tgttttacct tcttttgcta
     4801 attcagctgc tcgaattacc tgaaaagata atccttggtc agtggtatca ctgtcgataa
     4861 ccgttacttt cgtttttgtt agattactgg cttgtcttgc tgcttctact gttccactca
     4921 agccttttgt catatggatc gaaatcactt cactgccgtc ttctcctagc tcatcataca
     4981 tcgagatgaa ttctccaatt ggtggttggc ttgttttggg cagatttttt gctttcgcca
     5041 tcatttccat aaatctttcg ccgtctagtt ggtcatcatc gggatatact gttccgtcga
     5101 tcatgatgga tagcggcatg acacgaatgc ctaaagcatc gcgtacattt ttttccatcg
     5161 tacatgaaga atctgttacg atttttacat ttgtcattat tttctctctc tctttcttta
     5221 acaagatgcc ttattcatgt taaactacat gtaagacaat ttctattttt agtataacag
     5281 acaaataatg atttttcatt aattacacct agtaaaggag ccgttttcat ggaaaaaacg
     5341 aaatttaatc gaacgtatga aatattaaat gaagtgttta atgcagtcac ccatggaatt
     5401 gggactggat tgagtatcgc gggactagtg gttcttttag taaaaggtgc ccacttgcat
     5461 tcctccctcc atattgtctc ttatgctatc tatggatcga tgatgattct tttattttta
     5521 acgtctactt tgtttcattc attgattttt acaaaagcaa aaaaggtgtt ccaagtattc
     5581 gatcatagtt cgatttttct tttgattgca ggaagttata ctcctttttg tttgctgagt
     5641 atccgaggat ggctaggttg gactttattt gtattaatct ggttgtttgc gattcttggc
     5701 attatatata aagcgttgac gttgcataaa cgggagagag tatcaaaagt atctacgatc
     5761 atctatattt ttatggggtg gctctgtgtc gtggctgctg ttcctcttta tcactctctt
     5821 ggaatgactg gtgttatttt gatggtagca ggcgggcttt cttacacgat aggcgcattc
     5881 ttttatagct tgaattctat ccgctatatg catgttgttt ggcatttgtt tgttatgtta
     5941 ggtgcaggtt ttatgtactt ctctgtttta ttctttactt aataaaaaaa ataaagctat
     6001 ggatgggaca gctctaaata acagctagtc tatccatagc ttttttatat cgtcaattcg
     6061 cctgtcccgc tttttacagg attctcatta cgctgatacg tttcaaattg atgcggataa
     6121 cgattttttt catccatttc tccttgacta gtattgatca gtgaccaatc agaccagtca
     6181 acaggtggaa aataagcatc tccatcgaat acatgatgga tgactgtacg gtataatatc
     6241 gtacaaaatg gcagaaattc ttgataaaca gctgaccctc cagcgatgaa aaagataccg
     6301 tctgtttctt tttccagagc aagcacgtct tccacactat gcatgacatg cacgccttct
     6361 gctttatagg aagaatctct cgtcaaaaca atcgtctgac gattcggcaa cggacggcta
     6421 cccatccctt cgaatgtttt tcgtcccatc actagtgtat ttgcttctgt catttgtttg
     6481 aagaatttca gatcatttgg caaacgccaa ggaagacggt cttcttttcc aatcaatccg
     6541 ttttcatcct gtgcccaaat tgcagctaac attttctcat cctttctgat tctaaacagc
     6601 gataggagct ttgatcgccg gatgtgggtc atatccttct aattggatat cttccatctc
     6661 aaaatcaaat actgactgct tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc
     6721 tcggctcaat tgttctttca tttgttcgat atgattgata taaagatgag catctcctaa
     6781 tgtgtgaaca aattcgccaa ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa
     6841 cgcataactt gcaatgttga acggaacgcc tagaaacaca tccccactgc gttgatacaa
     6901 ttggcagctt agccgtccct cttgaacata aaattgaaac atcgtatgac aaggaggcaa
     6961 agccatgcta ggaacatctt ccggattcca tgcagaaacg atcaatcttc tagaatcagg
     7021 agtctttttg attgcttcga tcacttgttt gatttgatcg ataaagccac cgtcttttgt
     7081 ttcccagtga cgccattgat agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa
     7141 ggaatcatcg gttaggatct tctcgcaaaa aagcttgctc tgctttttat attcttcatt
     7201 gaattcagga tctgtcaaac atctatggcc aaaatcagtc atatctggtc cattatagtc
     7261 tggacttttg atgtagcgtt caaatgccca ttcatcccaa atatggtttt tattttgtaa
     7321 tagatagcgg atattcgtat ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc
     7381 aaaaggtact cgtttcgttg ttaacaaagg aaaccctttt tgcagatcaa accgcatttg
     7441 gtatccgaat aagcttaatg tgcctgtgcc tgttctgtct tctttacgat gcccttcttc
     7501 caacaatttt cttcctaagt caagatacgc ttgttccatc ctttttcctc ctaatcttct
     7561 acccattcac tcaagtattc ccaccgtgcc attttttctt caagttgtgc ttctgtttca
     7621 gatacgtcgt tttgcaattc ttgtaatcga gtgaaattat ctccttgatg attcatctct
     7681 tcttggagca atgagatttt ttcttcaagt tctgcaatct catcttcaat cgtttcccat
     7741 tctttttgtt ccatgtaaga taatttcttc ttttcagcag gttccttgct ctgtattttt
     7801 ggtttcgctt tttcagcagg ctgctgggtc tctttttgct tcagtaaata actggacatg
     7861 tttccgtaat aaagttcttg ttttccgttt ccttggaata ctaataactt atctgctacc
     7921 ttgtccaaga aataacggtc atgggaaaca gtgatcacag cacctggaaa acttctaaaa
     7981 taatcttcca aaatcgtcaa tgttgctatg tccaaatcat tggtcggttc atccaataat
     8041 aaaacattag gttgctgaat cagcagtttc aacaaaaata accggcgttt ttctccgcct
     8101 gaaagtttgc ctatcagtgt cccatgcata aatcttggaa ataaaaatcg ttcaagtaat
     8161 tctgctactc cgatttgact gccatctgct gtttttgctt cttcagcagc ttcttgtaag
     8221 taagcaatca tccgtttatc tggtgccatt tcttcatttt cttgtgtata atacgcaaga
     8281 tgaaccgttt caccgataga cattgtaccg ctatccaggg gaattctgcc agctagaata
     8341 ttaagcaatg tcgattttcc cgcaccattt ttaccagtaa tacctagtct ttcccgagat
     8401 tgaatcaata aatctaaatg ttctagcagt gtttggtcat ttatacggta actgccatct
     8461 ttgatttcca gtactttttt ccctaaacgt tgtgtcgcta ggttcagctc taaatcatct
     8521 tcttggttta cttggtacaa gttctccttt agatcttcga aacggttgat ccgtgcttgc
     8581 tgcttggtgc cacgtgcctg tacgccagcc ctcatccaag ctaattcctg tttataaagc
     8641 cgcttgcgct tttcttcttg ttctacttct acacgatcac gttctgcttt ttctaagacg
     8701 taagcttcat aatttccttt atactcgtat aattttccaa atgaaagttc gaagattcgg
     8761 tttgctactc tatctagaaa ataacgatcg tgggtaacca tcaatagtgc gccacggtat
     8821 tgtttcaaat agttttccaa ccactcgatc gcttcataat ctaaatggtt tgttggttcg
     8881 tctagtaata gtaaatcggg ttcatcaatc aacacttgcg ccagactaac tcgttttttc
     8941 tgaccacctg ataaagtact gattttttta tctaattcac tgattcctaa tttttgcaaa
     9001 ataatctttg cgttggtatc tgtagtccaa gcatcttctt tattcatttg ttcttctgct
     9061 ttagcataac gtttttgcac atcagggtct tctccgtttt cccctagttc gatcaatgct
     9121 tcctcatatg ctcgaacagt ttggatcaag gggctgtttc cttgaaagac tgcttggaga
     9181 acagttaatt catcggaaaa ttcagctgct tgagaaagat aaccaatacg gtaatcagtt
     9241 ggataaaaca ctgtttgccg atcaccgtcg ctgctatcga taccggcaat aatatttaag
     9301 agacttgttt tacccgtccc gttcgtccca atcaacccga tacggtcttt ttcgtggacc
     9361 aaaaaagaaa tctggtcaaa caaatctttt tcaccataag tttttttgag gtcagttact
     9421 tttaattctt tcaacatgtt ccctccttat cttggatatt gtatcataaa aatagtgaac
     9481 ttcaggaaaa tttttttggt atttgttctt tttgtgttct caaaaaaaga acaggaccga
     9541 ttcgatcctg ttctgatatg ttttgttttt tataaaactt gaaaattttt acctttttat
     9601 tttattggac tcgttttttt tgactttgct cgaaacgcaa atcggaaaga aaagttatca
     9661 gatggtttgt tcatcgtata agattctaac aaattgatat tcctgcgata gctgttcgca
     9721 gaaaacgtcg tgattttttc agcaacttcg tattcatcaa tgatttttct ttcaagataa
     9781 taacctctta gcattttttt cgtaaacaga ctatcttgct ggatcatcca cgaagaaatc
     9841 aaattccccg agttcatttt tattccttca agtttgcgtt gctggataaa aaattgaacc
     9901 atcgtttcat cataaatatc agtcaagtta tctaaacttt caataatcaa ctgagtattt
     9961 ttttgatatg tctctctgat ttcttggata tcttcatcac taataaatgt ctgacgatca
    10021 gcatatcttc tttgccaaaa cattttcgga tggagaacga ttcgcattaa cctacggaca
    10081 acaatgaacc aaaagccgat aaaagacaaa atctggcttg tgatagaatg ttcgaatcga
    10141 gaaagaaaac gggagtaaaa acgataacca ttagaagaaa gcttgcccga acgataactt
    10201 tcatccaatc catcccgttc gatcgacaaa atcagtgctt gtatttcttg tacttcttgc
    10261 ttttcatcat cggttaaatc ttccaagtac agatcacgta tccgttcttg ataattttct
    10321 atcactgctt cagtggctaa aaattctttt gtattacgat caatttcctt tatttctttt
    10381 cgcaaaactt caacgacctc ttctaaaatc atcaaagcat tcaaatccgt gcttttcgct
    10441 tcctctgttt ccgtcagcat aggcaagaca atgatcccta tgaccaaagt cactaagatg
    10501 acacccgccg tcagaaacaa aagcaaagac cgctggtaaa acatcatatt attgattgaa
    10561 aatggcaaaa taaaaatcgt agccaaactg actgttccct ttacaccgcc aaatgttaag
    10621 atcaagatct cgttcaactg ttttttgaac tttttactgc catctttgaa tacataaaaa
    10681 agactgatag aaatgaatct gatcacaaaa agcatgacag agattaatac gataatcgct
    10741 aataaaagac cgtttggata taaaccgtct ccccatactg gcgagaacac ttgcgtcaat
    10801 tcgattccta aaaatataaa tactaatgca ttcagtgtaa aagcaatagt tgtccatgta
    10861 ctgtgggaca agctcgacaa ttctgcatca aacacagaga ttttcctaaa accagaagct
    10921 tggagaatac ctgctgccac tgcagcaatg attccagata catcaaatgc ctctgccaat
    10981 acataggcta gaaaaggaag caaaagttcg atcagcagat accctgtgac atcttgtgct
    11041 gatgcttttt cgatcaaatg aattattttt tgtttgaacc agacaagcaa aaagccgatc
    11101 aaagctcccc caatactaga aactactaga ctcatccccg cgtttactgc tgaaaaagat
    11161 ccggtgatca gcgcaccaag agcaaactgg aaggcagtaa caccagatgc atcattcaaa
    11221 agaccttctc cttccaaaat atgcatcgcc tttttaggga tattcactcg tccagaaaga
    11281 gaactgactg ctaccgcatc tgtcggacct aacgctgctc caaaagcaaa gcaggcagca
    11341 agtgggacac ttggcaacag gaatgataac gtcgcaccta ccccgacaag ggtcaaaatg
    11401 actcctccaa atgccaagaa caaaattgtc ccgaaatttt tgagaatcga agaaatatct
    11461 gctttttctc cttctctaaa taacaatggt gcaataatca taactaagaa caattcaggt
    11521 tcaaaatcga ttgattgtcc ccactctgtc agacctaata ccactccaag gaaaatttgg
    11581 atcagtggag ctggaatcat tggcaaaatg cggctagcta tgttcgataa ggtgatcgtt
    11641 actgcaaata caataactaa ctcagcaaat tccatcgtcc atccccttta ttttttattt
    11701 tgtgtaagca tttttaaaaa ttgttctttt ttctttaaaa ttttggtttc gactacttca
    11761 acctgtgttt ggttcccctc taattcacac tgtacacgtt ttgactccaa acgaagcaga
    11821 tcatgtttca gacgttccaa ctgacgatac atcaatatat ttcttttacg caacttacaa
    11881 agtatttgac ggcggtcatc catcgaaagg gcgccatagc gatcatgcaa cagttttttt
    11941 tgttgttcaa aactctctct cattttccag atctcctata cacaaacttc catcattata
    12001 gcaaagttct ttttggatga aaaatatttc attgtttcat cgtttattgc tagttagaaa
    12061 tttccataaa tttttcaacg cctcattcta ttccaaatag tatcagatat caagtaaaac
    12121 ttaagaaaga taaaagtttc tgacaaaagg atgtttactt tcccattcct gcctttcttg
    12181 ttcttttatc acaacaaaaa aatggggctg cgacaaaaat cttgtcacag tcccttattc
    12241 tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc tctttcacag
    12301 cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat cttctacacg
    12361 ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt ttgttgattg
    12421 atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca caagatcagg
    12481 tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca atgcgcgatt
    12541 aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg cgtccccttc
    12601 atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt cactgccatt
    12661 gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat gacggcggtt
    12721 gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt ctttactcat
    12781 taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag cacaaattta
    12841 tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac tttaagtagc
    12901 caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt tcgataaaaa
    12961 atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt tttcagctaa
    13021 ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta gcaagatttt
    13081 accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg attctcctac
    13141 ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt gcagtgcatg
    13201 ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc gttgtcgtag
    13261 tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc acgatttcaa
    13321 aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg cccatgcttg
    13381 gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg cttcgccttc
    13441 ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat ggattcccgc
    13501 ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt ctacagatat
    13561 tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct ctaatttgaa
    13621 ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt catggaaccg
    13681 ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca gaccatcgaa
    13741 caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa aatctcttct
    13801 tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac ggtagtcctg
    13861 ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt ctaataccaa
    13921 cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt tgatttcttc
    13981 tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca agatatcacg
    14041 aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt tcagaactgg
    14101 aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa cctttcctgc
    14161 gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca agtccgtttc
    14221 agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct caaagcgatt
    14281 atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta agtcgatatt
    14341 caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt ctgctaccga
    14401 gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga cttctcttgc
    14461 caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga aataccccac
    14521 cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag aacgcttttt
    14581 atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc ctagataaga
    14641 gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa atggaaattc
    14701 tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac agtaaaagaa
    14761 gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta cttgaacatc
    14821 gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag agctttattt
    14881 ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt actaggagga
    14941 aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc tttgtcattc
    15001 tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga tctactgatc
    15061 gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta ccgcatgggt
    15121 ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta cggcatcact
    15181 atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt aacctatatc
    15241 gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg tgtgattgat
    15301 tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa caaaagcgaa
    15361 gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct ttctggagaa
    15421 ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc tagtgaatta
    15481 actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc aatcatcaat
    15541 gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag attactgaaa
    15601 aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc cagctgacct
    15661 ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta cccggttact
    15721 tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt tcaaaaaagc
    15781 atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt ttagttttgc
    15841 ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt cactgactga
    15901 agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc ttgtctgcca
    15961 agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac ggatttcatg
    16021 aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca tgggttcccc
    16081 ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc cttcaacaaa
    16141 gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc aaaaaacttc
    16201 tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct tttcccgtac
    16261 atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag tgcctccata
    16321 tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt cagctgccct
    16381 tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga tagttcatga
    16441 taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt ataagcatgg
    16501 gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat cacttgtatc
    16561 acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa tgtttcatct
    16621 tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg tctatcatgc
    16681 agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg attttctttg
    16741 attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg aagagcttgt
    16801 cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc ttccagatag
    16861 ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg gaacagtcga
    16921 tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc ttcgaattct
    16981 ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga aatttcattt
    17041 atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg atcaactgaa
    17101 aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa cccagctgct
    17161 tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa atcagctatc
    17221 gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag gtattcaaaa
    17281 gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt cagtccgtca
    17341 ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat caaaaagcca
    17401 atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc ttttaatgct
    17461 ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg caacattttt
    17521 tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt gaaataccgt
    17581 cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta tacctatttt
    17641 ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt ccaaatactc
    17701 catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca gccacacaaa
    17761 aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga aatgaaacat
    17821 cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg tcagataaaa
    17881 agcttgttca aacagtttac cataatttga caggttttct gcctctaatt gataaaaata
    17941 gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt tatttggata
    18001 gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac agataaaagt
    18061 caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat cataaacgat
    18121 gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca aaatcgtccg
    18181 ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc atagtgatga
    18241 atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt catttccaaa
    18301 tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa gcaggttctt
    18361 taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat tgtttgcttg
    18421 atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct gattcgttaa
    18481 aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg tttctccgta
    18541 aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca tccctcctct
    18601 tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc aaagctcacc
    18661 tcataacttg caaataaata aaaattaaat aaaatacatg tgttttctat gtatgacata
    18721 cggattcgga atctctgtct cttatttatt ccttttctgc taggaaataa aaagaaaagc
    18781 acttgtagcc ctagagccac aagcgctttt tcaactgtct tatttaacag cgtcttttaa
    18841 tgctttacct ggtttgaatg caggtacttt gctagctgga atttcgattt cttcaccagt
    18901 ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact tcaaagttac caaaaccgat
    18961 taattgaact ttttcgccgt tagctaaagc atcttgaata gttgaaaata cagcatctac
    19021 tgctgcagtt gcatcttttt tagttaaatc agtagctgaa gcaacttttt cgattaattc
    19081 tgctttgttt gccatggata gattcacctc ctccacaaga gatatgatag aaatatcatt
    19141 tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa atattgatta acttcaatat
    19201 tctgttatca cgatatcata tgaaccgctt aatagcaagg tttttaccaa ttttcacatg
    19261 ctttttttag tcatagaaac gtaacaaacg aaactgtctt tttcttttga aaaaaaaacg
    19321 tcgatatggt aaaatgccta ttttctccta cgagggataa ttcggatgga cgtcccttca
    19381 aaagtaaatg cttttctgat ttgattttct aagaaacgtt cataagaaaa atgcatcaat
    19441 tcctcttcat tgacaaaaac gacaaatgta ggtggtttga cagcgacttg tgtagcataa
    19501 aaaatcttca ggcgttttcc tttatctgtt ggtgttggat tgattgctac agcatccata
    19561 atgatatcat tcaacacagc cgaagagata cgaagattct ggttcatact tacttgttcg
    19621 atcatttcag gcagacgttc caaccgttgt ttagtcactg cagaaacaaa aacgattgga
    19681 gcgtaatcaa gataccggaa ttcatcacga atctcttgtt cgaaatcacg cattgtattg
    19741 gtttctttct ttacaagatc ccatttattc acgacgataa taattccacg accagcttca
    19801 tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc cttcttcagc attcagtacc
    19861 atcaaaacga tatcagaacg gtcaatggca cgcattgcac gcatcacgct gtatttttca
    19921 gtattttcgt atacttttcc gcgtttacgc ataccagctg tatctatcat taagaatttc
    19981 tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc gggtcgttcc ttcaatatcc
    20041 gaaacaatta cacgttcttc tcctaaaatc gcattgatca aagaggattt accaacattt
    20101 gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct cttcttcagt ctcttcagag
    20161 aaatacttca ctgcttcatc taaaatatca ccaatcccta gtccatggct tcctgaaaca
    20221 ggataaggat cgcctagccc taaggaataa aattcataga tatcgtttct catttctgga
    20281 ttatctactt tattgactgc taaaatcact ggtttattac ttctgtataa aattttcgca
    20341 accatctcat ctgcgtctgt cacgccttcg cgtccgctga ctacacaaat gattacatct
    20401 gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat ccataaaagg ttcatcgcca
    20461 agatcaattc cgcctgtgtc gatcacgctg aattctcgac ctaaccattc accttttgca
    20521 taaatacgat cacgagttac tccaggagta tcttcgacaa tcgagatacg ctcaccagcg
    20581 atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc caacgattgc aattgttgga
    20641 tttgccatat tatttcctcc tcacaagttt ccaatctcac ttagtttaac gaatgcattg
    20701 ccaactttca agcatagatt gtcaattaaa cgaagtattt ttattcctta ttgaaatact
    20761 aatcgctttt caaaagaaaa actgagacaa aagcagctgc tttcatctca gtcacagata
    20821 caaaatcaat tatttttcag aatcatcggt tgctttttct tctacttctt ctttcaacgc
    20881 atcacctaaa atgtctccca tagtaaatcc agtattttct tctggtaatt cataagactc
    20941 aacatttttt ggttcttctt ctgaagctgg tttttcttct aaagctttga tactcaatgc
    21001 aatacgatgt tcttctggat ggatctctaa tactttcact ttcacttcgt caccttcatg
    21061 aagcacttca tgtggtgtcg caatatgttt atgtgaaatt tgagagatat ggactagccc
    21121 ttctactcca gggaacactt caacaaaggc accgaagctt gtcaaacgtt taactgttcc
    21181 gtctaatact gtgccaactg gagcttttgt atcgatttct gaccaaggtc caggctgtgt
    21241 gtctttgatt gacaaggaaa cacgttcttg ttcaggattg atagacaaca ctttgacatt
    21301 tacatcatca ttgacagcca atacatcact tggttttgct acatgactat gtgaaatctc
    21361 tgagacatgg actagaccgt caattccgcc taggtcgaca aatgcgccaa aatcagtaag
    21421 acgtgcaaca cgtccactga gaacatctcc ctcatggata gttgaaagaa tttctttttt
    21481 cttttcttct ctttcgtttt ccactaccgc tttatgcgat aagatcaaac gattttctga
    21541 tggttcgatt tcgatgattt taaagctcaa tgttttgcct ttatagtctg aaaagtctgc
    21601 aacaaaatga tcttccacca ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac
    21661 taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg accttgccct cttggaaatc
    21721 tctttcgata tcttcccaaa cttttttagc atctaaacgg cgttttgaaa gtagatagct
    21781 gccgttttct ttatctttgc cgatcgtact gataacgact aaatctaata catcaccgac
    21841 ttttactact tcgctgatat cttctaccgg caaagttgat aattcttttg ccggaacgac
    21901 tccttcaact ccagcccctt cgattccgac aatcacttgt ttgtcttcaa tgactaaaac
    21961 ttcgccctta acaacatcgc caacttttac ttcttgtacg cttttcattg cgtcttccat
    22021 tgactggttg ttctcttgat tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa
    22081 cattctgccg taagtacggt actgttttta gtttaattga ttattcaaaa aaaataaagc
    22141 gatttcgttc gttttccttg aaaaaacaat tgtttttcat tttttttctt aatttaaaaa
    22201 agaagataac cttttttcaa aacaacagct tttattgctg caacgacttc tggaatcgat
    22261 ttgccagtcg tatcgatacg aacagcatcc tctgcttgct tcagcggaga gacgtccctt
    22321 gttgaatcat aatggtctct ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga
    22381 attccttttt cttggttctc tttgaaacgt ctttctgcac gctcacttac gcttgctact
    22441 aaaaagattt tcacttctgc attcggtaat acagctgtcc cgatgtctct tccgtccatg
    22501 acaacgccac cgcgttcccc tatttttcgt tggacagcaa ctaattcttc tctgactttt
    22561 ccatgtgcag agacttcaga aacagctttt gtgacatctg gttgacggat ttctaaagtc
    22621 acatcttttg atgctatgaa tacatgctgt ccatcttctt gttgcgaaaa agagatagga
    22681 tactttttga tcagcgaaac tagcccttct tcatctgaaa aagatacatt gttcgtaata
    22741 gctaaataag ttactgcgcg atacatggca ccggtatctg tataaatata attcaattct
    22801 tttgctaata tcttggcaac ggtacttttc ccagatgaag caggcccgtc gattgcaata
    22861 ctgatttgtg tcatgatata actccttttt ctcagaaaaa aagccgcaaa gcgcggctat
    22921 tattatttaa ttcttaattc ttgaccaggg taaagcataa agttgtttgg atctaagcca
    22981 ttcaattcaa acaattgatc agtggtgatc cctgcacgct cagccacttg ttttggacct
    23041 tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg atgaagaagg tgttgtttct
    23101 gctgcattgc tgcttgaatc ccctgcttga taagttgcct cacttgagga agtcggtgtt
    23161 gtctctgctg caatactgct ttctgacgtt acacctgtat cttgagacac agtagattct
    23221 tccgcgattg tactagattc tactgttgaa gtctcagttg actcctcttt tgttgtactt
    23281 gtagaacttt ctactactga tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg
    23341 cttgatccat ttctgatcca gaaataagca cctgctggaa tagcgataca taagaccagt
    23401 aaaatcaaaa atagcgaaag aaataatgta tttcctttct tttgttgtct ctgagaactt
    23461 cttgatgaaa cttcatcatc tgtatcgtag attggttgtt cccatggttc ttgtgtttcg
    23521 ttattttctg tagattgatg tctatctttt ctgctcactg ttttacctcc taaactctat
    23581 cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt
    23641 ttttaaataa atttagtaaa atcgttcccc aatgaagttg ttcagtctta gaaaaaagct
    23701 tcctctttgg cagaagttct gcttttgctc catcaatatc acaacaattc tgcggctttt
    23761 cggagagttt ttcgccaaaa taagctaaga tgaattttct gcggcagttt tcttcattaa
    23821 tatagcgaag catctgctgc aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt
    23881 ccacccaatt ttctggtttt tcagactgct ggatcagctc catccatttt ttttgcagct
    23941 catcaaaggg agcttgctct gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt
    24001 ctctagaaag ttgattaaag aaataatgga tacgttcatc cccactttga taaaaaagaa
    24061 ttgctgcact ttcttcctga tctctgcctg ctcgaccgat ttcttgtaca tagttttcta
    24121 gactgtctgg caaatcataa tggaccacat aacggatatc agatttatcg atccccatac
    24181 caaatgcatt cgtagcaatg agaaactgca gctgattttt tacaaactgc tgttgaagtt
    24241 gtcttctttg agctgcatca agaccaccgt gatagtatcc aactgtaaaa cgttccctga
    24301 ataaatgata caatcgttcg acttcttttt ttgttgcaca ataaatgatt gccgctcctt
    24361 gtgctctttc catgaagtgt tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa
    24421 gcgagatatt ttgccgattg actgattgtc tgatctcttt tggtggtcga tcaaatagta
    24481 catgacgtat atctcttgct actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg
    24541 ggtttcctaa aagctttcgg atttttccta actgttgata ttctggacgg aaatcaactc
    24601 cccattgaga cacgcaatga gcctcatcta ctacatataa cgcaatattt tgttttttca
    24661 gatgctctaa aagagaagga ttagtcaaca tttcgggact caaaaacaaa aatttatatt
    24721 gcgatagatg acggaggacg tatcttcttt cagattgcga aagtaaacta ttgaaagcag
    24781 ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg atcttccatt agagaaagca
    24841 gcggtgacac gatgatgacc agaccttcca ttaaataccc cgtcaattga tagcaaaggc
    24901 tttttcccgt tcctgtaggc aaaatagcga gagtatcttc tccatcaagc aacgcctgaa
    24961 taatttcttc ttggccggga cgaaatgaat caaatccaaa atattttttt aattcattta
    25021 ttaacattct gtatcgcttc ctttctcaat agatagattt gatacaaacg aaattctcca
    25081 taatctaatt gtccatctac attccattcg ctaaagcgcc actctcgcac ggaatcctta
    25141 tttggttcca aacgttcgat tgtttctgca gagagaatcc actcgtatgg aaaatcatca
    25201 aaaaacaagg cccactcaat aagatgatct gttactgttc ctctttttaa atgacggatt
    25261 gccatgatct ggtcaattgt ctgaccattc ataaacatct gcttggtgat catcatgctt
    25321 tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga atctggccgt
    25381 ttttcgattt gtgctaacaa aaggtggata catctgcttt gttgcagata aaccgctgac
    25441 tcatcattgt tttttgccag ctgataagga agcagccctg ttcgctcatt tccagataat
    25501 tggttagcta aaaaatctgc tgcttcttct ggtatctccg aaaacagctg caccaaactc
    25561 tcatacgcag tatcaatcaa aatttcacgt ggcaattggc tttcactaag ccattttttt
    25621 agttggaaag tataaaaagg acttgtctct gctggaagat agtcttgaat acccgttgcc
    25681 agattagaaa ttacttgaac agcaaattta atgagccgcc aactagtttc ccctgttctg
    25741 ccatagcggt caaaacgcaa accagtatgt tctatttgat gttcggacaa catatcagcc
    25801 cctgcaggcg ttagctttgc ttcattttca tttatttcga tccaaccttg ctgcgcaatt
    25861 ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg gaaaactgtt atggataaac
    25921 aaaagttcgt gggtaaatcc ataaataagt actgaagtcg ttcgttttcc cacaaggagt
    25981 tggtaaagag aactaatccg cagcttgtgt cctgatgaaa atagcgataa aataaaaaca
    26041 atcatattat tctccttaag ttaggatggt tagaaatgtc actactatgt aaacttgtac
    26101 ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc gccagatatt ttcgattatg
    26161 atgaagaagg aatcgtttta tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc
    26221 cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg tccagttcga gcgattttat
    26281 tagaaaataa aatataaata taaacgaaac tttaaaaacg tacactaagt tctctaaact
    26341 tgaaaataag ccgggatctg tttctcttgt tccgtgaaat cccgacttat tttgaaagat
    26401 tcatttgcta tctatagtta attgactagt tttttcacgg accctatagg acccatttat
    26461 attatttcaa ctttttcagc cagttttcca atgtattgac gatcacagtt tgctggtctg
    26521 cgttagaaat ttctgcctct ccatctcctt tttgctcacc atagctgcca aaacctccat
    26581 gatttccgcc attgattgat acatactctg ttctttccgg taaatactgc ttagcttctt
    26641 gataagcttc ctgattcaat actttatctt ttgtcgccgt gatcgataaa acaggaacag
    26701 ctgattctgt caatgttcct tttttgtcgg gataacttgc catgaagaat acccctttta
    26761 aatgtttgtc tgaatgattc ttattgacat aacgacttgc cattacgcca cctaatgaat
    26821 ggccaccgat cacataatcg gaatttggaa aatcttcttt gaccttatcc gctttgtttc
    26881 cgcccatcac tgctagatca agtggcattt tgacaatagc gactgaaaag ccgttttttg
    26941 ctaaatcttg cgcccaaata ctataacttt ctggctctac gagagcacca ggataaaaaa
    27001 taagtaatgg agactttcca tgattctttt tctctccttc aaaaaataag taatcttctt
    27061 tgttttttcc ttcttttacc aacacttgcg cttctgacga tggttcgtac gtattttgct
    27121 gtatatagta ataaccggct ccaccgagta atcctaaaat gatgactaag ccaattagta
    27181 ttcgtctaaa tattttcact ttctcccacc ttttctatct gagtgacccc tttttcttaa
    27241 tcaagtatga ttatacctta aaaaaataag agagaaatgc aaactttcca gcatcaaata
    27301 aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt tttatttagc
    27361 acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac tacttaattc
    27421 ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg caccgaatgc
    27481 cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg gttcagtcat
    27541 gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac tgtctagtgg
    27601 cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt tttctgctgt
    27661 tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc tcatatctac
    27721 accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg ttagctcgcc
    27781 ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt cttgaactaa
    27841 ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa ttgccagacg
    27901 agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac caagaacgac
    27961 gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc
    28021 agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg ctcctccagc
    28081 ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc
    28141 tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt
    28201 ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa agataaaacg
    28261 aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat acattgccgc
    28321 aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt cttttatagt
    28381 tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa cgtccatatg
    28441 acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt aatagccttc
    28501 tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt tatccaaagc
    28561 ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga
    28621 aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt gttctgtcac
    28681 aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca aattcctccg
    28741 acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc aagcagaatg
    28801 ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac ttatccacgt
    28861 gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt acctgtattt
    28921 gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac aaataatatc
    28981 gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc aattgctaca
    29041 gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga tgcagattga
    29101 taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg tcccatgatt
    29161 cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat agggatatag
    29221 aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc ttgttcgccc
    29281 caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat accacgagca
    29341 acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa ttcagagaca
    29401 aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac taagaataac
    29461 atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc tagaaccgta
    29521 cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt gaaattcgca
    29581 ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat aaagaacaat
    29641 acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc ttcaatatcg
    29701 aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg atctttttcg
    29761 atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat aacgaacatc
    29821 aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga agctacgcct
    29881 gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac taatacgatt
    29941 gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag taaagcatag
    30001 aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa taacagcatc
    30061 aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga agcaatcccg
    30121 gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac caaaatgaag
    30181 aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg tacacctagc
    30241 atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg ttgtactcgt
    30301 tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc agctggtata
    30361 aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata cgctgaaggc
    30421 atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt tccgttagag
    30481 acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa aaataataac
    30541 acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct cctctttttt
    30601 caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta taaacaaaca
    30661 ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg atttttatgc
    30721 ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg catgtcttgt
    30781 atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt tatttgactg
    30841 ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat
    30901 gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa ggaacgatcc
    30961 caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc agccctaaaa
    31021 acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa atagttagag
    31081 cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt ttctttacac
    31141 taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta gcagtgtctc
    31201 ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa cgcaagaacg
    31261 ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc gagatatcac
    31321 taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa
    31381 ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg gtgttcttca
    31441 tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc tctccaaatg
    31501 aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat
    31561 aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc gattcagcta
    31621 tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac cctgcccctg
    31681 aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat tcaaaaaacg
    31741 caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca aactgctgac
    31801 ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc
    31861 gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca tatttttcac
    31921 ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc
    31981 cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct ttattccttt
    32041 tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa cgtaaacttt
    32101 atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag
    32161 caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt cttttaccat
    32221 cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag aaataactcc
    32281 tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga cttcatccac
    32341 ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt gtccgtttac
    32401 ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga tgccagcgtg
    32461 ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat tccatcaaag
    32521 aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt
    32581 tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc atataatatc
    32641 ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc agctagacgc
    32701 tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct tgtgattggc
    32761 tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt gtttgatgtc
    32821 ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa tacataatgg
    32881 ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga ttcttttaat
    32941 tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct taattcttca
    33001 ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact taatttcgtc
    33061 atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt ctttttgttg
    33121 cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag tgacaacttc
    33181 acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat ttctttttat
    33241 acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt
    33301 ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac ggatcaaatc
    33361 gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc catcgatgtt
    33421 cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca gtctttcagc
    33481 tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc gaggatcttc
    33541 ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt
    33601 gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta gctcgagtgt
    33661 tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg gtatatcata
    33721 tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac cctcgaatac
    33781 atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt ctttgctagc
    33841 cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc cgggtacttt
    33901 tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga cggattcaaa
    33961 gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat aatcccttga
    34021 atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac
    34081 gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa actcaatagt
    34141 cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc accttcctat
    34201 gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt cgtgatatgt
    34261 gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac tgtccgcaaa
    34321 tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg tggtgttact
    34381 tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat tccttgccga
    34441 gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc ctttacaagt
    34501 aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc acctaaagga
    34561 atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga aagatgaaga
    34621 tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata catgacttcc
    34681 aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt
    34741 tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg cgtatcgata
    34801 tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg gaatcttctg
    34861 agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg caacttctcc
    34921 agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt
    34981 agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat
    35041 ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat aataatcccc
    35101 ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt ccataatctg
    35161 agcaaagaaa aagccgtcta gacactcaga cggactggtt tcttttttct ttttcatagc
    35221 attcctgaca gattccatgg aacgttagcc gatgatcttt tactaaaaaa tgataacgtt
    35281 cttctactac ttgctccact tcaagtaaaa gatcctcttc tacttcctcg atcgtgccgc
    35341 agttcaagca tagtaaatga tgatggaaat gtttggcgcc ttcttttctc aaatcataac
    35401 gggctaatcc atcattaaaa cttactttat cgacaatttt caattctgtc aagatttcaa
    35461 gggttcgata aacagtagca agtccaattt ccggactctt ttttttcact aaaaagaaga
    35521 tttcttctgc agataaatgc tctttctcat tttccaaaag cacaagcaga gtagcttctc
    35581 tttgcggtgt cagcttaaac cccgcttcat gtagctgctg ttttgttttt ttcatcgccg
    35641 aaatagcatt catgaccatt tactcaaccc caacaattta taataattac aaataaaaaa
    35701 agatataaca ctatgttaat taaaattatt ataagctagt taagaaaaac atgcaaggga
    35761 ttctcaatta gattttttga ctagttttgt cacaccgttt tcttgtgtaa tcaatccttg
    35821 tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg ctgataccaa aagctttcat
    35881 gatttcttct ggatccgatt tatctgtaaa gttgatttgc tgatctgcag aacgttctaa
    35941 aatggttaaa atcatttgtg catcatctga tatagcttca tatgctcttg gcttcaatga
    36001 aagattcagt acaccgtctg gtcggacacc aatcactcga ccttttatct gctcgcctag
    36061 acgaggttca ttatatcgtt cagaaggatg gataaaccca agatagaaat cttccgttaa
    36121 aacgtacgta cctgttaatt tcaagcggta tgctgttcct atcacatctt tgtttttcat
    36181 ttcttctgtt ccatatttgc ttaaagattt gaatattttt tcatctgcta aactgcccca
    36241 aatacggtct tttgcatcaa ctctaaggga aatcatcaag cgatcccctt ttttaggcca
    36301 tagctctgtc attgtcggca attcatcaag agatacgaca aagtctttat ctggaagacc
    36361 gatatcgaca aacgcaccta aatcttttcg tgtagcagta acgattccaa aagcgtaatg
    36421 accgatacgg cttttaggaa tttgtgtagt gatccgattt tcatgttttt gattttggta
    36481 accgaatcct tcaactgctt caccgatttt gtgttcacct tctgatttgc ttaagccaaa
    36541 ggtttgtccg tttttttgga caaagtagtt tttttcattt tcatcaataa taatacctgt
    36601 aaatatagtt gctaataaat cgttcatttt tttctccctc tatagtgaaa ggttttctta
    36661 atacatttct ttgctatcat aacataatgt tatctacttg aatagctgag ccttggtttg
    36721 gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac tccccttttt cgaataaacg
    36781 gtgttaaacg gcaggctcac agcctctttc atttgcctaa aattaattgc tcccatttcc
    36841 tccgaaccat ccagttttac agttgatggt tacgatgtaa aatggcaaag cactttctcc
    36901 aatgcctttc atcaatgcag attttgttga agtggcatat agattataat agccttcccc
    36961 attttcgatt ctggcttttt ctaatctgta acctccttca acgatatatc cacgttggat
    37021 acatgtagca atcactttgc tttggacacc tggtgcccac gtccatgctg gattagatgc
    37081 atcggcaata tcacttgctc tggcagattt gattggctga ttcgctgagt ttgaagagga
    37141 aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc
    37201 ttgagctgca gctgctgctt gagcggcttg ttttgcttgt tcttcagctg cttttttcgc
    37261 ttcttctgct tttttctttt cctcttcttc ttttttctct ttatcagtga gcatcttatc
    37321 tacttgtttc aattgttcat tcaactgagc agatagatct gtatttttca ctttttctac
    37381 tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa tccactactt ttccgtcttt
    37441 aaagactata gctgtttttt ctttagctgt ttgcaattca gcatattgat ttttagcttc
    37501 tgtttgtgct tgttgaatca attttccaaa atctgtgtta tccgtttcga ttgcttggat
    37561 ctctttatct gctttcaact tgatgtcatc tgctagatga tcatcgacga tggcaggtgc
    37621 tatgaaccac tcattcactt tttggatatc tcggatctta gattggatat cttggtaagt
    37681 tgtttctaac tctgaatatc ctttttcatt cttaagactg gataaatcat tttttagttt
    37741 tgataaatct tgatgaatca tatctgcacg aataaattga tggtgatcat ctgtataaaa
    37801 ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt ctttttgctt ctgctgctac
    37861 ttcggcttct actttttgat gatggtcata ataagcccag cttcctccac cgagtagaag
    37921 tactgctgct aaaacgagat aaggtacttt ttttgatttt ttcttttgcg ctttatctgg
    37981 atctgttttt tctaagtttg attcttgatg tgttttttca gtatctggca cgattgttat
    38041 tgatttcgct tctgcttcaa acagcttagg ctggttctct tctttttcta tttttgcttc
    38101 aagatcagta ccctctttgc ttacagattg attttcagca tcggattcac tatttatttc
    38161 atcgggctct gttgactttt tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg
    38221 cagtttattc gttttttcct tttctaattg tttttcagta aatacttcag ttttttctgt
    38281 gatatccgtt tttacgtctt ttatttttac tgtttcttta cctgtggcgt tttctctatc
    38341 agagtctttt tcagtttctt gttccttttc tggctctact ttgttttcct gtgcaatttt
    38401 ttcactgatc attttgagca gctctttcgc gtgctcttct tttgtatctt cacgatgtgc
    38461 tttgatataa gcggatagta tcgaattttc ttcctcatta tcgacgattt ctttcagttc
    38521 agctactctt cttttttgtt ttgattcaag cgtttcttct tgattctttc ctgattcttc
    38581 cgaagtctca tgaacagttg ctttcttctg ttcatttttc tctatattag ttacttgatc
    38641 gtgagcaggt ggttcttcgt cttctggttc tgctttttcg aataattcca ttacagattc
    38701 tagaggtaaa tctttcaatt ccgaccattc gattggatcg tttaattctg tatcaggaaa
    38761 gactgcatct tcagttgatt tatcctgcat agctgcatct gtatttgttt cttcaattga
    38821 ttcttttatt tgctgatctt ctgcagttat ttgatgaccg cagtctggac agatagtatt
    38881 ctgctccatt tttttcgatc cgcaatttgg acattttttc atcacttagc gatcggctcc
    38941 tttactaggt ttacgctacc atttaatcta tcatattcta tacgtcacgc caatagctta
    39001 tttttaggat ttacttaaaa ataatgaatg aatttccttc ctatagaaaa aaaagcttga
    39061 gactgcgtag tctcaagctt tttttctttt aaaataaacc aaacagtttt actgatttct
    39121 cttctggtca atagtccacg cttattctaa gaaatctttt aattgttttg aacgagaagg
    39181 atggcgcaat tttcttaatg ctttagcctc gatctgacga atacgttccc gagtaacacc
    39241 aaatactttc cctacttctt ccaatgttct cgtccggcca tcatctaaac caaaacgtaa
    39301 gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt acatcttcca attgttcttt
    39361 tagtaactca taagctgcgt gttctgctgg acttgttgct tcctggtctt ctatgaaatc
    39421 acctaagtga gagtcatctt cttcaccgat tggcgtttca agggaaacag gttcttgcgc
    39481 gattttcaag atttctcgga ctttttctgt tggaagatcc atttccgcac cgatttcttc
    39541 tggcgtaggt tctcttccaa ggtcttgcag caattgacgt tggatacgta tcaatttatt
    39601 aattgtttca accatatgaa ctggaatacg gatcgtacgt gcttggtcag cgattgctcg
    39661 agtgatggct tgacgaatcc accatgtagc ataggttgaa aacttaaatc ctttgcggta
    39721 gtcaaatttt tcaactgcct tcatcagtcc catgtttcct tcttggatca agtcaaggaa
    39781 ctgcatgcct cgtcctacat aacgtttagc gatacttact actaaacgaa ggtttgcttc
    39841 tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc
    39901 ttcttccgca gttaaaaggg agacacggcc aatttctttc agatacatac gaaccgggtc
    39961 attgattttt acaccggttg gcgcagaaag atcttctgtt tttgcttgtt cagctttttt
    40021 ctcagcactc ttcaagctgt gaattgatgg atcaccattt tcatccacca cactgatacc
    40081 tgcgtcttca atcttttgga tcaatttgtc catttcatct gcattcaaag tgaatggtgt
    40141 agcaagctga ttggataaat catcatatac aacttgtcgt ttgggtttgt tttctttgat
    40201 gaaagctgct actgctgctt catacgtttt tttatctgtt ccatttgcca taaaagaagg
    40261 ccccccttga gtagctattt ggcttgtttt aactgtttcg ttaaattgat gatttcaatc
    40321 gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg cttcttgctg catttgtttt
    40381 tttaatgtta tttcatcagc taagccagat ttgctgatca catctagcaa gtcttggatt
    40441 tctcgtggcg aactttcttc tgccaccggt aaatattcga tttccaccac caaccgcctt
    40501 aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata atgttgtgag
    40561 acatatgcat cgaataaaaa ataaacttct gcatatgcat catgaataaa ctgaaattct
    40621 cgttctttta aaatattacg gacagaaact tctttaaatg cacgatataa tagaatctgc
    40681 tctgcctttt ctacttgcgt caaaggtctc tttttccgtg cagtttcttg tctcgtaacc
    40741 tgagtgtgtt gactttcctg aggttcttga cgattttgct gatttgtcct tcgaagatcg
    40801 cgcagttgtt tttgtatcgt ctctctgccg atctgaaact ctgttgacag ttgagataga
    40861 tacatatctt gttcgattgg tgaaacaact cggctaagtt caactagtaa ttcttcgagg
    40921 tattcgatct tgtctttttc attatctaaa tttttccctt gttttaaata acgtgattta
    40981 aaagtaaaga ctgtttcgcg cccatgagcc actaattctt ggaaagatgc ttctccatac
    41041 ttacggagat attcatcagg atcaagcttt tctggaacga ctacgacaga aagtgctaat
    41101 cgactgtttt gccgaagcaa ttcaatccct cgattggttg cttcaaaacc tgcgttgtct
    41161 ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca tttgttggat ctgttgagct
    41221 gttagacttg tccccataga agctaatcca gttttgatac cagcctgcca tgcagcaatc
    41281 acatccataa acccttcgaa caacaaaatt tctccgtttt tccggatatc gcttcttgct
    41341 ttatcaagat taaataaaac ttgtctttta ttgaacagtt ctgtttctgg actattcaag
    41401 tatttgggct gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg
    41461 atcgtctggc cttgagaatt acgaagtgga aacatcactc tttgataaaa acgatcagac
    41521 atacggccat catcatgcgt gacaaacaat ccagagcgtt ctaacaattc atttgaaatt
    41581 gattcattct tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg
    41641 aattcttcaa ttaattctaa agacaaccca cgactttgca aatattctaa agctggctgt
    41701 ccagcttttg tatgcaatag catgtgatga tagatttccg ctgctttttc atgagcagca
    41761 atcagtttcc ccgtagcaga ttcaggattt cggacagcta tagtacgctg acgccattga
    41821 tcttctaacg gtatctgttc gaagtctgcc acttttacca ctgcttctgg aaaacttaga
    41881 ccttctagtt cttgaagaaa agtaaacacg tttccgcctt taccacaacc aaagcaatga
    41941 aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg aaaaggacac
    42001 agccccaagt aatttttacc agactttttc aactggacat attgtccaat cacctcaacg
    42061 atattcgttt gactgcgtac tgtttcgata acctcttgag gaatccgttg agccattttt
    42121 tcaccccctg ctctattgat atcttttggg tttaacagaa gatatttcag gagaataacc
    42181 ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa ggacacaatt atacatgttt
    42241 gattgtatca tatttcattg ctttttcaag tataacacat ttctatctca ttaagcatca
    42301 atttgctact cttttcagta actgattttg aatatcagcc ttccagtaca atactcgaag
    42361 aacaatattt acgagcatga atacaaagaa gtatcctacg ctctctccca accctaagtt
    42421 tacttccaaa attccatgga acagcattgc agctacatat atccctacga tcgtcgttac
    42481 tgctaggaag atgcgcaatg ggttgaaggg ataacaagct tttacaactg ccatgcaact
    42541 gatcccaatc agcaagtaat acatcaaagt agtagtttca gcatttcccc atgcgaatgt
    42601 ttttccaagt agatacaccg caatgatatt cactactaca agcaaggcat ttggtaaagc
    42661 attaataagt gctgtaggca aataacggta agtgattttt cttttatctt gttcaaaaga
    42721 taaaaagaag gaaggatacc cttcaatcgc taaatcaatc agcgtgattt ggatagggat
    42781 aaacggaaat ccaattgctg taacggcaca tataatcgat aaaataaatg aataaatcgt
    42841 tttgataaaa aagatgcctg agactttcgt cacattgttg accactcggc gtccttcaaa
    42901 caggacttct ggcaatgtag taaaatcaga gtccaacaat accagattcg caatctgacg
    42961 tgttgctcca tcaccttctg ccatagcaat gctgcaatct gcttctctta acgccaaaac
    43021 atcattcaca ccgtcgcctg tcattgcaac cgttctgccg ctttctttca attcatttac
    43081 cagcaatttc ttttgctgcg gactcactcg tccaaaaacc gtatagcgat gagcagcttc
    43141 tcgcacttcc atttcttcag taatttgaga aagatcgaca tacgcatcat aattcggtaa
    43201 tcctgcacgt ctagcgatat tggagacagt aaaaggattg tctccagaaa tcactttcag
    43261 atccacacct tcctcatgaa gataagctag tgtttcttga gcgttttttc ggattggatc
    43321 atcgatttca aacagagcaa ccggctcaac aaatgctggt ttttcttcgt tcaattgctg
    43381 atttggagcg atccccaaca taagtacgcg atagccattc gcttgggctg tttttagact
    43441 gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct cctacgtaca cgactcccag
    43501 ttctggaaat tccatcgcgc cccatttgcg atcagaagaa aatgcgactt tgtttgtcac
    43561 atcataccga ttagaaagct caaaataatc tctcatagcc cgcatagtga tgttgttatc
    43621 actgctttct gccagatacg tccccataat ttctgggatg gctttctcgt acagcggatg
    43681 tagtgcttct acccgctgga tactcatttt cccttcagta atggtccctg ttttatccaa
    43741 gcataatgta tcgacatggg ccagcgtttc aattgaatac atgtcttgaa caaggattcg
    43801 tttttttgcc agtttggtta cccctgtcgt taatgcaata cttattagca ataccaaacc
    43861 ctttggaagc atgcctaaca aagctgctgc agatgcaata acagaaattt tgatagaagc
    43921 atcgcggatg aacagtgctt ccaaaaatag tatgatcccc aaaggaataa tcacgaaact
    43981 agtgaatttg gatactttgc gaatcgaatc tacaagttca gaacgaatag ctttgtgagt
    44041 ctttgcctcg ctagtcaagc ggacagcata attatctgca cctactcgga taacttcagc
    44101 catgacagaa ccgcttgtca aaaagcttcc agaaagcaat tcagctccag tttctttgac
    44161 aatcaaatca gattcgcctg tcaaaagagc ttcatttgct tctgcccgtc cagatataac
    44221 ctgtaaatcg gctggtatct gttctcctgc tccgattttt accaaatctc ctaataccag
    44281 ctctttagta gaaatcgatt gttctttacc gtcccggatc actagtactt ctgattggga
    44341 aacaatcgat aatttccgta caagatttct tgcatgtatt tcttgataaa ttccaataac
    44401 gatattgacg acaataattg ctaaatacgc catgttggaa tacgcaccta caaataacag
    44461 acaaatccca ataccaaagt tcagaaaatt gaataacgtc aatatattat cgctgaatat
    44521 ttgttttgta ctttttgatg catcttcctg gtagtcattt tgctggccag aagccatttt
    44581 ctgaactacc tcttgctgtg taagtccatt cattttcttt ttctccattt tccgccaact
    44641 ttcaaaaata atcacgtgtt taagtctagc gtgttccaag attaaaaaca acttgttttt
    44701 ataaaattta tagaaaaaca cacgaatgat tttacaaaaa agatgccaac ctttttgttt
    44761 gaatcacccg tgtgctgttt ttattatcaa tctatcttta cttatattgt tcctccaaac
    44821 ggctcatcca ccaacctagt gctgtaccgg ctataaaaag aacaggacaa gctagtaccc
    44881 cagcaactcc caatcccgcg taatcggtag gaatgatcat aatcgttgaa gcaaaaacta
    44941 cacctaaaat gaaatggaat agcttgctat atgcctttga gaaaatgtaa tccatgattt
    45001 ttgacaatcc aagaacacaa agaatcccac caattccgat cggcacaatc acttcaagtc
    45061 gtacttcttt aaatccgtca gccatcgctt tatacaatcc catatacacc aaaaaattag
    45121 aagggctcaa tcctgggacg atcataccca aaccaatcaa tccgccggca atcagccatg
    45181 ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc gccgtataat aaaaacaaat
    45241 aagccagaac aaaagtaaca accatgatga tcacatcaga agtatctctt ccttttttcc
    45301 ctgcttcttt ccataacgca ggaacagttc cagcgataca tccgacaaaa aaccataata
    45361 tcgttgtttc atacgcacct aataaaaaac tgactaaaaa agaaagtaga aagatgcctg
    45421 tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa
    45481 ggtgggctaa aaaagaaatc agccgctcat acatgccaaa gacagctgct aatgcaccac
    45541 cgctgactcc tggcaagata aatcctgaac cgataaacat tcccttcaca aaccgtaaca
    45601 tccagtcttt ggttgtgtac cccacttctt gattttccat tttttgcctt ttttccacta
    45661 tttgcgtact ccttctttca tttaatttta ttcatgattg aacatttcct gctattttac
    45721 ttaaagactt tttcataggt ctctattatt ttttcattca ttttgacaat atcaggataa
    45781 aacatgttac ctccattatg atagagatag ccgccattgt aaagcaagga ctggatccgc
    45841 caatatcggt attgtttccc ttcctcattt cctaaaagtg ggctcagcac atctttagaa
    45901 tacgcttctg ctaattctac tgtgttttct ccgccatgat tttttacata ggcaatataa
    45961 tctttcccaa aattatatgc ttggacagct gtccataaat cgcatccttg tttctttgct
    46021 tctactatca tttccgccaa atatttgaca ccttgatcaa tactgtcagc tggattatca
    46081 atgatctcag cttctccatg cagactttcc gagctctgca tgagatctgt cccttttcct
    46141 tttgattcgg tcaatattat cgccttgatc aaattctcgt agttttccat atgataattt
    46201 tttgcagctg ttgttatttc tatttgatag ctttccactc gttttaccga acgataagtt
    46261 cttcctcata aaaacaacac cgcaccaata accgtcagaa tgatcatgta aaaaagaata
    46321 cggataatag ataatcgttt tcgcttcatt tcatcaccta gttattttga catctttttt
    46381 tatcttacta tttctttatc ttcttggcta tttcttcctt gatttctgtg tacgccttaa
    46441 gaaaaaatga attttcctgt attttttgac atcttacgaa aatgatcgtt gtttttcagt
    46501 aaaaacaatt gcccaatgac tgatataatc gttatacttt acacagtagg tgattatgtg
    46561 aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg aaatttacta tgaaaagagc
    46621 ggtcaaggct tccctctctt tttactgcat ggcaatgacg gcagcggccg ctttttttca
    46681 gaacaggtcc ctgtactcga acgctactat accgtgtatc tagtagatag ccgaggacat
    46741 ggtcgttcga caaacgaagc atctatgttg aattttcagt taatggccga ggatctgaat
    46801 acgatcatgt tgctggaaaa aatcgatcag gcagattttc ttggatttag tgatggagca
    46861 aatctagcac tggtatttgc cagcagcttc cctaaaaaag ttcatcgatt gattttaaat
    46921 tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca gagtcatcag taatattcat
    46981 tatgcatggg tatggttact ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt
    47041 atcaaacttt tgctgcatga catcgggctt acagaaaatg atttaaaaaa aatcaactcc
    47101 cccactttga tcatcgtggg aaaaaaagat gtaatcaagc taaaacattc tttgtatata
    47161 gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag aacaaggaca tgaattagct
    47221 agaaaagatc ctgaacgctt caatcgagaa gttcttcaat ttctttctga aacataacta
    47281 ttaggaatag aaggtgattt atttgttaaa aaataccata caatggctga aaaaccacct
    47341 aggactgttc aaaacaatct ttttgatttc tgtcattgtc attattgtcg gtgaattgat
    47401 gtccatcggt aaaactctat ctatccagca attaggagaa acgtttgcca ctatacctgt
    47461 atggaaaagc ggactaatgc tattgattgg tcttatctct gtgttaccta tggttggtta
    47521 tgatattatt ttgaatcgga tgcttgaaca aaagcagaat cctcgttacc tgtttgagac
    47581 aagctggctg atcaatacga tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg
    47641 gcttcgctct gaactatatg gacaaaagaa agagagcaga aacgtgatcc aagcattatc
    47701 gaaagttttc ttgtttttga tggctggact ttctatttac agtctgcttt cttttttact
    47761 tgtagtcttc acgccagttg ctcctttttt gaagcaatat tggatatggt tgatcggcgg
    47821 cggtctatat tttcctgtcg tctttttatt tactactttc aaaaagaaag gattccttgg
    47881 tggtacctca cttaaaactc aaggacagct attgctagtt tcattgttgg aatggaccgg
    47941 tgtcctgttt agtttccttt ctgttggtta tttaatggaa atccggattg atctgtggca
    48001 aacaattcca ttattcattg ctgcttcagt gataggaatc gtgtctatga ttccaggaga
    48061 aattgggagt ttcgatgtaa tgatgattat cgggttgtcc gctattggtg tgccaagaga
    48121 aatagtcgtt gtgtggatct tgctctatcg cctgttctac tacatcattc cgtttttgat
    48181 tggaatcgtc tttttcttca aaaatatcgg ttccactttt gatcagcgtt attctggtat
    48241 ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg gttgtcttat tatacttttc
    48301 tggaatcatg ctagtacttt ctgccactat cccgcaagct tttacagagt ttcgctggct
    48361 tcatagtttg aacccattga aatttcattt tattatccaa tttcctagta tactattggg
    48421 atttctttta atcattatgg gcagagggat cgcagcacgt gtgaaacgag catatttgcc
    48481 tacaatcttc ttgattgttc tagctttact ttatgtttta ttaagtgatt ttagtttcac
    48541 accggtggtt ttcttgtcta ttctgcttct gatcattctt gcttctaaaa acgaattatt
    48601 tcgggaacaa ttgatctatt catgggagtg gcgtacgatc gatggaatca ttattggtgc
    48661 actgtccttg ctttacatcg ttattggggt atacaatctg ccggatttcc ctcatcggcg
    48721 tcaccatttt atttcctttt tcttatttcc ttctgaaaag atttggtttt cgggattgtt
    48781 ggccatcatc gctgttagtt tcatgatcgt tctatttgtt catttccttc aaggagaaaa
    48841 gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg aagatcctta caacttatgg
    48901 aggaaactcc gatagtcaat tgatcttctt aaaagataag cggatgtttg cctatgaaaa
    48961 agacggtgaa ccaactgttc ttctccaatt cgcttgcttc aataataaat gtattgtaat
    49021 gggtgatcct tctggtaaga aagaagattt tccagaagca atcgaagctt ttatcgaaga
    49081 aacagatcga ctatgctatt taccagtttt ctacgaaaca agcgaagaga ttgttatgat
    49141 tttgcatgaa ttcggctacg atttcattaa gatgggggaa gaagcttatg tagacctgaa
    49201 tagttttaca acttcaggga aaaaaatgaa gggcactcgt gctgtcttga atcggatcga
    49261 acgagaagga tttacatttg atgtcttaca accaccattt tctactgagc aaatgagtac
    49321 tttcaaaaat atctctgata attggttggg ttcacgaaag gaaaaagggt tttctttagg
    49381 ctttttctct gaagattatc tacaaagagc gcctattgca gttgtcaaaa atacccaaga
    49441 agaaattgtc gcttttgcaa cgatcatgcc tacttacacg aacaataaag taggaacgat
    49501 tgatctgatg cgatatgatt ctaaaaaagc accttctggc agtatggatt tcttgttctt
    49561 gcatttgttt gcctatatga aagaagaaca tatccaatgg ttcaatttag ggatggcccc
    49621 tctttcaaat gtcggaactt ccagaaagag ttttttacag gaaaggattg catacttggt
    49681 ttacgagttt ggttctcatt tttattcttt ccatggatta agagaatata aaagcaagta
    49741 cgctacaaac tgggtctcac gttatactct atattctaga gatagttgga ttgcatatgt
    49801 tatgattgct ttgctgatta ttgataatgc gccagttgag cagacacaag gtagaataag
    49861 tggtttgaaa aaatggatac gaagaaagta ttaaaaactg cataccgata gatgaacact
    49921 ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc atgcttctac aatctcttct
    49981 tatacaattg aagaacaaag agcggtccaa acagaaatcc tgtttggacc gctcttttat
    50041 tttaaacatt tttcattacc aaaatattcg agtcgcgttt cttatgcttc gatctctgta
    50101 acgatacctg aacctactgt ccgtccgcct tcacgaattg agaatgtcgt tccgttttca
    50161 acagcgattg gatggatcaa gtcaacatca atcgtcacgt tgtcaccagg catgaccatt
    50221 tctgtatctt ctggcaatgt gattgttcct gtaacatcag ttgtgcggaa gtagaactgt
    50281 ggacgatagt tgttgaagaa tggagtatga cgtccacctt cttcttttgt caacacgtac
    50341 acttctgctt tgaattttgt atgtggagta atggaacctg gtttagcaag tacttgtcca
    50401 cgttcgatat cgtcacgttg gatcccccgt aacaacacgc ctacgttatc cccagcttcc
    50461 ccgtaatcta acgttttacg gaacatttct acacctgtta caactgcttt ttgtgtttca
    50521 ggtttgatcc ctacgatctc tacctcatca ccgacacgaa cagcaccacg atcaatacga
    50581 cctgaggcaa cagttcctcg acctgtaatt gagaagacgt cttccactgg caatagtaat
    50641 ggtttatctg tatcacgttc tggtgttggg atatattcat ctactgtatc catcaattcc
    50701 ataatagcag cttcagcatc tggatcgcct tgcaatgctt ttaatgcgga acctttgatc
    50761 acaggagtgt catcgcctgg gaaaccatat tcgctcaata actcgcgaac ttccatttct
    50821 accaaatcga tcaattcttc atcatcgact aaatcaactt tgttcaagaa aacaatcagg
    50881 tattttacac caacttggcg tgacaataaa atgtgttcac gtgtttgagg catcggaccg
    50941 tcagttgcag aaacaactaa gattgcacca tccatttgtg ctgcccctgt aatcatgttt
    51001 ttcacatagt ccgcgtgtcc tggtgcatca atatgcgcgt agtggcgttt ttctgtttca
    51061 tattctacgt gtgcggtatt gatcgtaatc ccacgttcac gttcttctgg agctgcatca
    51121 atactagcat agtcttgagg atttgctagg ccttttttgc ctaatactgt agtgatcgca
    51181 gcagttaaag tagttttccc atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt
    51241 ttacttctgt cataatgttc ttttgccata atttacaaac ctcctaaata atatagatca
    51301 ctgaaaaatt tcagctctca cctttttatt atagtctttt tttgactaaa tcaaaaagaa
    51361 tacgtccgac ttactttttg taagattcga actaagctta ttatacgtca atattggtca
    51421 aagtcaacta agagcactcg tctgtcttat acgcgaaaaa agtacctttt ataaataaaa
    51481 aaatgtgttt tttgagaaaa acttcaagat agtccttaac tttcttttga agcaataaga
    51541 acaaaatgag gaaaagttta aaattcagct gttgccaaca actttcaata gacgaaatct
    51601 tctatttcgt taataataag gaaagaggtg aagtacatga aaaaattaat tggcgtactt
    51661 gtcgttttag gcatatttct cggcggaagt tttgcagctt atcattattt ttacggcggc
    51721 gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt cagctaccca agcagataac
    51781 ggagagaaat tcacgactta tacctacaag caagcggctt ataataaaga tggtgaaaaa
    51841 aaacaggtga cgcttcgcga agaaagagaa catccattaa agatgaatgc gtatcttaaa
    51901 ctcaaagtca atcctcgtaa aggtgtatta agctgggaag aaataaaggc ttctgaagtg
    51961 cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt ttattagata
    52021 aaaagaggct gggacag
//
LOCUS       DAZKNM010000003        49992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000003 DAZKNM010000000
VERSION     DAZKNM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.003"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1457
                     /locus_tag="ACG8M9_000109"
     CDS             237..1457
                     /locus_tag="ACG8M9_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0376916.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1454..4174
                     /locus_tag="ACG8M9_000110"
     CDS             1454..4174
                     /locus_tag="ACG8M9_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0376917.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4158..5102
                     /locus_tag="ACG8M9_000111"
     CDS             4158..5102
                     /locus_tag="ACG8M9_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0376918.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5171..6181)
                     /locus_tag="ACG8M9_000112"
     CDS             complement(5171..6181)
                     /locus_tag="ACG8M9_000112"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0376919.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6361..6705)
                     /locus_tag="ACG8M9_000113"
     CDS             complement(6361..6705)
                     /locus_tag="ACG8M9_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0376920.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6698..7132)
                     /locus_tag="ACG8M9_000114"
     CDS             complement(6698..7132)
                     /locus_tag="ACG8M9_000114"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0376921.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7270..8007
                     /locus_tag="ACG8M9_000115"
     CDS             7270..8007
                     /locus_tag="ACG8M9_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376922.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8000..9211
                     /locus_tag="ACG8M9_000116"
     CDS             8000..9211
                     /locus_tag="ACG8M9_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0376923.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9415..10197
                     /locus_tag="ACG8M9_000117"
     CDS             9415..10197
                     /locus_tag="ACG8M9_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0376924.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10267..10929
                     /gene="trmB"
                     /locus_tag="ACG8M9_000118"
     CDS             10267..10929
                     /gene="trmB"
                     /locus_tag="ACG8M9_000118"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0376925.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11027..12781)
                     /gene="ade"
                     /locus_tag="ACG8M9_000119"
     CDS             complement(11027..12781)
                     /gene="ade"
                     /locus_tag="ACG8M9_000119"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0376926.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12913..13842
                     /gene="comGA"
                     /locus_tag="ACG8M9_000120"
     CDS             12913..13842
                     /gene="comGA"
                     /locus_tag="ACG8M9_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0376927.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13799..14848
                     /gene="comGB"
                     /locus_tag="ACG8M9_000121"
     CDS             13799..14848
                     /gene="comGB"
                     /locus_tag="ACG8M9_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0376928.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14845..15123
                     /gene="comGC"
                     /locus_tag="ACG8M9_000122"
     CDS             14845..15123
                     /gene="comGC"
                     /locus_tag="ACG8M9_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0376929.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15098..15568
                     /gene="comGD"
                     /locus_tag="ACG8M9_000123"
     CDS             15098..15568
                     /gene="comGD"
                     /locus_tag="ACG8M9_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0376930.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15540..15887
                     /locus_tag="ACG8M9_000124"
     CDS             15540..15887
                     /locus_tag="ACG8M9_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376931.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15850..16275
                     /gene="comGF"
                     /locus_tag="ACG8M9_000125"
     CDS             15850..16275
                     /gene="comGF"
                     /locus_tag="ACG8M9_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0376932.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16247..16606
                     /gene="comGG"
                     /locus_tag="ACG8M9_000126"
     CDS             16247..16606
                     /gene="comGG"
                     /locus_tag="ACG8M9_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0376933.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16778..17785
                     /locus_tag="ACG8M9_000127"
     CDS             16778..17785
                     /locus_tag="ACG8M9_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0376934.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17810..18994
                     /locus_tag="ACG8M9_000128"
     CDS             17810..18994
                     /locus_tag="ACG8M9_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0376935.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19431..21014)
                     /gene="abc-f"
                     /locus_tag="ACG8M9_000129"
     CDS             complement(19431..21014)
                     /gene="abc-f"
                     /locus_tag="ACG8M9_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0376936.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21119..21247)
                     /locus_tag="ACG8M9_000130"
     CDS             complement(21119..21247)
                     /locus_tag="ACG8M9_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376937.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21592..21735)
                     /locus_tag="ACG8M9_000131"
     CDS             complement(21592..21735)
                     /locus_tag="ACG8M9_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0376938.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21958..22437)
                     /locus_tag="ACG8M9_000132"
     CDS             complement(21958..22437)
                     /locus_tag="ACG8M9_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0376939.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22510..22794)
                     /locus_tag="ACG8M9_000133"
     CDS             complement(22510..22794)
                     /locus_tag="ACG8M9_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0376940.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22872..23066)
                     /gene="ssrS"
                     /locus_tag="ACG8M9_000134"
     ncRNA           complement(22872..23066)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8M9_000134"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23138..24424)
                     /locus_tag="ACG8M9_000135"
     CDS             complement(23138..24424)
                     /locus_tag="ACG8M9_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0376941.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24562..25233
                     /locus_tag="ACG8M9_000136"
     CDS             24562..25233
                     /locus_tag="ACG8M9_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0376942.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25199..25687
                     /locus_tag="ACG8M9_000137"
     CDS             25199..25687
                     /locus_tag="ACG8M9_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0376943.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25700..26647
                     /gene="prmA"
                     /locus_tag="ACG8M9_000138"
     CDS             25700..26647
                     /gene="prmA"
                     /locus_tag="ACG8M9_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0376944.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26648..27412
                     /locus_tag="ACG8M9_000139"
     CDS             26648..27412
                     /locus_tag="ACG8M9_000139"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0376945.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27645..29858
                     /locus_tag="ACG8M9_000140"
     CDS             27645..29858
                     /locus_tag="ACG8M9_000140"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0376946.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29869..30315
                     /gene="dtd"
                     /locus_tag="ACG8M9_000141"
     CDS             29869..30315
                     /gene="dtd"
                     /locus_tag="ACG8M9_000141"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0376947.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30486..31745
                     /locus_tag="ACG8M9_000142"
     CDS             30486..31745
                     /locus_tag="ACG8M9_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0376948.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31834..32781)
                     /locus_tag="ACG8M9_000143"
     CDS             complement(31834..32781)
                     /locus_tag="ACG8M9_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0376949.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33175..34467
                     /gene="hisS"
                     /locus_tag="ACG8M9_000144"
     CDS             33175..34467
                     /gene="hisS"
                     /locus_tag="ACG8M9_000144"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0376950.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34482..36251
                     /gene="aspS"
                     /locus_tag="ACG8M9_000145"
     CDS             34482..36251
                     /gene="aspS"
                     /locus_tag="ACG8M9_000145"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0376951.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36360..37235
                     /locus_tag="ACG8M9_000146"
     CDS             36360..37235
                     /locus_tag="ACG8M9_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0376952.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37501..39384
                     /locus_tag="ACG8M9_000147"
     CDS             37501..39384
                     /locus_tag="ACG8M9_000147"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0376953.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39459..39743)
                     /locus_tag="ACG8M9_000148"
     CDS             complement(39459..39743)
                     /locus_tag="ACG8M9_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0376954.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39733..40467)
                     /locus_tag="ACG8M9_000149"
     CDS             complement(39733..40467)
                     /locus_tag="ACG8M9_000149"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0376955.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40596..41219
                     /locus_tag="ACG8M9_000150"
     CDS             40596..41219
                     /locus_tag="ACG8M9_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0376956.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41411..42532
                     /locus_tag="ACG8M9_000151"
     CDS             41411..42532
                     /locus_tag="ACG8M9_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0376957.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42539..45667
                     /locus_tag="ACG8M9_000152"
     CDS             42539..45667
                     /locus_tag="ACG8M9_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0376958.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45686..48880
                     /locus_tag="ACG8M9_000153"
     CDS             45686..48880
                     /locus_tag="ACG8M9_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0376959.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49026..49733)
                     /locus_tag="ACG8M9_000154"
     CDS             complement(49026..49733)
                     /locus_tag="ACG8M9_000154"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0376960.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16298 a         8804 c        10436 g        14454 t
ORIGIN      
        1 atggagcata aattccagta caaatagtac ttcttcagtg tcaaacgaag cgaatataac
       61 atagatattt aggattgtga caaaaatcat gtcacaatct ttttttcgaa taaacttatt
      121 ttttgaagtc ggccatttat cgtaatttct ttgcataata cgaacaaatt ttcggttatt
      181 gatgaaaaaa aacattgaaa atggtatgat aaaagaaaaa aaggaggaaa tttttaatgg
      241 tgaaatttat ccatgcggca gatttacata tggatcggtc atttgaagga ctgacaactc
      301 ttgataaaac tgtacaggaa aaactattga agacgaatct gaccgtttta tcaaatatca
      361 ttgaacaggc aataataaat aacgttgatt ttgttttact tgcaggagat aattttcatc
      421 aaaatcgtcc ttcattaaaa atacagaaac atttttcaga acagatgaaa cggttagaaa
      481 agaaccatat acctgtattt ataatattcg gaaatcatga tttctatcaa aaagagcggt
      541 attggttctc ctttcctaaa aatgtccatt tatttacatc cgaaacggta gagacaaaaa
      601 aaataacgat aaaaagtgga gaaactgtct ctctcagtgg atttagttat cgtcaaccgt
      661 ggattcaaaa agacaaagtg atggaatttc cttctagaga actcacagat taccatattg
      721 ggttgtacca cggggaacct ggcgtctctc aaaaaggaaa ctatgctcct ttccaaccct
      781 caaagatgca agaaaaggga tacgattatt gggctttagg gcatatccat gtgccaacgg
      841 tcttaaatga aaagcaaaca attgtttatc caggtgcgcc tcaaggtcat actaaaaagg
      901 aacaagcttc tacatctatt ctactagttg aattaagtaa aggaagttgc caaatacatc
      961 cgattaaagt agcagaggtc tattggaaga caaaggaaat atcattgagg aaagcaagaa
     1021 cgacaaaaga ggtcattgtt cacattcgtc agcagctttc aaaagtagag gatggattta
     1081 gtttaattga gataaaatta aaagattatg accatctcaa tacagatgtt ttggaacgga
     1141 tacaatcagg agagctatta gactatttgt tagaggaatt ctcagatagg ataaatgatt
     1201 tctttatttg gcgtatttct ctcatagaga atacttttta tacgaagacc ccacttgctg
     1261 cttctgctaa attgatggaa cagttatttc aatactatca gacacctcaa gatttccaac
     1321 aaattttaaa tgaagtatat agtcatcaag aagcggctag aatattgagc gaactgccgg
     1381 aatatcaaga agaaacactt gaaaaagcca agcagctgct taatcaagat tttttatttg
     1441 aggaggatca ggaatgaaga ttttaaggat cgagattgct gcttttggaa aatggcgtca
     1501 aaaaagtttt gacttttatt caggcaatca gctgatctat gggggaaacg aagcaggaaa
     1561 atcaacgatt tatcaattta ttcaagcaat attatttggt ttcccttcaa aaggccgtaa
     1621 aaaaaaggat tatacaccaa aagacggctc agcctatggc ggcaaaatat ggcttaaaca
     1681 tcctgtatat ggtgaatttg ctgtcgagcg ctacaagcag caaaatcgcg gaaaatcgaa
     1741 ggtgtggctg ggggatcaag tgggaagtga tgagttacta gaaaaactga tcgcaccttt
     1801 gacgaaagag ttattccagc aggtatttac tttccagcaa gagcagttga tggaactaga
     1861 tcatttgaga gaaaaagagc tacatgatgc gctgatttct ttaggcatca cgggcagttc
     1921 tcaagtattc caaaagcggc aagaatatta tcagaaagca caacaattat tcaagaaaaa
     1981 aggccaaaaa ttactaatca atcaaaaatt aaatgaatgg aaagagctgc aagaaaaaat
     2041 tcatcaaaaa caagaacagg aagcttattt tggcaagttg atccaacaag aacaggaata
     2101 cgagtcgcaa cagcagcagt tattggcaca attaaaagag gcgaacaaac aattttcatt
     2161 agccagacag caagaactta atttttcttt atatgaagaa tggcaatctc tcaaaaaaaa
     2221 cagcactaca ggtcttcctg atgagcaact tcttttagat ctggagtctt tctccaaacg
     2281 gtatcagcaa acaaatgagc gtttggaaga attagaagca acactggaaa agcatagcgg
     2341 gatggatcag cagtcagcca gatattattt ttatttagag caggagcagg aactaaaaga
     2401 tctccagcaa cttcaattta cagcggaaca attaaacgaa gaatggcaaa gattacaaat
     2461 tgagactaga gaaacaaatt cagaattgaa gattttggaa caaaggtggc attggcagaa
     2521 aaatccgtta ccggaagaat ttttatatga agaagaatgg aagcatcttc tggctgaaga
     2581 aagaaggatt gctgatctag aaagccagat ttctgtgcgt ctccaaatag ctaaagaaca
     2641 gcaagagatg attgaagcag aagtgaatga cttggaagag acctatccag cattattaca
     2701 aaatcagtca gagatagcac cagtctcttc atctaaaccg tatatgctga tggctggcgg
     2761 agcaataatc attgcaggta tatttgtacc aatgcccttg aaagtgatcc ttttactaat
     2821 tggtgcaggc atgattggct atttcttcta caaaaggcaa ccaaaaatta aaactaaaaa
     2881 tgatttctct gagataaaag gaatgtggca agaaaaacta ggacaacttg atctagcctt
     2941 agctcaatta gctgaagcag aagaaaaaca aaagagaact ggagaagaaa aacagattct
     3001 tcagcaagaa gtatatcgtt ttgcggatca tcggcactta ggaaagatgg atacgctcaa
     3061 actcatgaaa gaatatggac aggaagtcat agattatcag aaattatatg gcgtatttga
     3121 gcggaaacat aaaagagaac aggaaataca agaaaaacta cgacaaatcg accaaaaatt
     3181 tgagtttgtt aaagaatggt taccgataca agataagaca atttcagaaa aaatggtatt
     3241 gctttcccgt tttatacaag agatggaaca gatgaaattt gccagaagct atcagcaaaa
     3301 tactttattg aagcaagaaa tcaatcaatt gaaaaagaaa caaaaagtat ggatacaaga
     3361 atacagcgaa caattgactc aagcaggctt atcttatcct tcggaagtac ctgtcttttt
     3421 acaaaaagcc aaacaagccg aacagcagat gaaaagatac aatgaattat cggaaatgct
     3481 tactccacta tttcctgaag agctgacaaa agagaaatta tctgtaagac tgcgccaact
     3541 gaaagaaaag caatcccaac tccagcaaga agaaaaagaa gcaagtgaaa atcggcaacg
     3601 actccaactt caagtagaag agttgcaaag agacggcaca ttggacgaac tttatcaaca
     3661 agctagccgg caaaaagcaa aattacagga attactggag gaatggggag cacttgaaat
     3721 cgccgggaca ttcttaggtg atctttcaac agagatgagt gaaagacaat taccccagtt
     3781 gttaagagca gcaggtgatt atttaggtat tttaacaaaa ggaaattatc agaagatatt
     3841 actgaaaaat gatacactga ctttaacaga cggatataca gaatttccca tctatgagtt
     3901 atctacaggt acgaaagatc agctgattat ggcgatgaga tttgcttacc tctcattaga
     3961 agctaaccgt tcgatatgcc cgatcatcat tgatgatggc tggcttcatt atgatcatca
     4021 gcgaaagtat caattagctg aacttttagc taaatttgga gaaaagtatc aaataatttg
     4081 tttttcttct gataaagaaa tggtaagcta ttaccatgag atgaaccaaa tcgtaaaaca
     4141 attagaagga gcaaataatg aaaaaaattc gtgatttagt aatcgatgag cagtttgaac
     4201 aatttgtcct gatcaaaagt gcagatgtaa gaatcgctaa aaacggaaaa aaatttattg
     4261 cttttacttt ccaagatact tcaggtacta tcgatggtaa atactgggat gcttcggagg
     4321 aggaaatcaa taaatttaca gccggcacag tcgtttttct aaacggtaaa agagaggttt
     4381 atcaaggaaa ccctcaagta aagattcttc atatgcgctt aacaagggta gatgagccag
     4441 gagagcctag tttatacatg gaaagagccc ctttgaagaa agaagaaatg gaagaagaaa
     4501 tcaatcaatt gttgtttgag atcaccaatg cgcattggaa tcgtattgtt cgctatctac
     4561 ttaataaata tcaaaaacaa ttttttgagt ttccggcagc aaaaagaaac catcatgcat
     4621 ttgccagtgg attagcttat catactttga caatgcttca cttagggaaa tcaatcgccg
     4681 atgaatatcc tgagctgaat cgagcattgt tgtatgctgg tatcattttg catgatttag
     4741 gaaaggtgat tgaactttcc ggtcctatgt cgacagaata tacgcttgca ggaaatttga
     4801 tcggtcatct ggtattgatt gacgaagaaa taactaaagc ctgtcttgcg ttgaaaatca
     4861 gcgaagctga cgaagatgtt ctcgtattac gtcatatggt tttagcacac catggcttat
     4921 tagaatatgg ctcacctgta cgtccaagaa taatggaagc agaaatatta catcaaatcg
     4981 acaatatcga tgcttcgatg cagatgatgt tgacttcgat tcgtcaaaca gaaccaggaa
     5041 aatacaccga tcggattttc ggtatggaca accgaagttt ctatgtacca aaagatttat
     5101 agcaaaaatt tatttttttc attcaaaaca aaaaaagcga gacataagtc ttcgcttttt
     5161 ttgttttctt ttattttgat gaatcagttg tttcttcagt tgaagattct tttgttgaat
     5221 cagttgattt tgtgtcagaa ctctttgttg aagctgtagt ttcacttgag tctttcgtac
     5281 ttgatgaagt tgttgtgaat gcactcaaca cattttcaaa ggcatcatct ttgattttta
     5341 catttgcatc ttttaattct tcaccgatta ctttagttgt gaatgcattg tcacttagtt
     5401 tagtttcagt agcgatatct tttagttgat ctttgtattt atccatgtca ttgcctttat
     5461 tttggttttt caccattttg acaacgtagt attctgtcgc ataagaagta ggatttgttg
     5521 tagtaataac atcggagatc tcaccgtctt tcaatttgaa agctgcttct tttacttctg
     5581 caggaatagt tgttgtcgtt gagtcgaatt taactttacc gccatcttct tttgtctctg
     5641 tatcagttga tttgtctttg gctaatttag agaaatcatc tccatcatcc gctgattttt
     5701 tcacatcttt tgcttcatct tcacttgata atttgatgat ttgtgcttct acttctggat
     5761 ggaaagattt ccaagcagtt ttcaaatcgt catctgtaat atctacgtga gctttcaagc
     5821 cagcttcaaa agctaaattg ttacgaatat attctttata agtatctttc gtatatccag
     5881 cagcttctaa ttgtgattca aaagtatcac ctaatgattt cgcttgtttg tcgtattctg
     5941 catccacttg cttatcggtt actttatcgc catatttgtt attgaatact ttataaatga
     6001 tcatccgttg tactaaggat tggttcgatg attcaagttt tgcttcatca tagaaatcag
     6061 atacagtgat cgttccgcct ttcatcgttg cgatatcttt attagtatcg cctgaacaag
     6121 ctgctagagt taaaacagcc aacgcactag tagctgccaa aataattgat ttttttttca
     6181 ttaaaatttg cactcctgta attagttatt atttttatat attaactata ccatatccca
     6241 tttcctttaa aaacgttgtc gtggtagttt caaacaaaat tcaaaatttc atcacattcc
     6301 taggaaatac gcaaactttt ctcacttata tagttaaatc tgttgagaaa ataatcatta
     6361 ttatatttgt tcaggatact ctggcagatc agcttttagt ttttctactt gttcattgat
     6421 ttgtgtgata cgtggttcgg cttggaattt aaaatcttcg atatcttttt tgattgcttc
     6481 ttggaaagca ggaatcacag attcagctgt ttcttttgtt tcgatcaatg cgcttttaaa
     6541 atgattcagg ctgttattta aatcatttgt cagagttgtt gcttcttcca attcttcgat
     6601 cagtttcttt cttgtttcat tcccgctcct gggcgccaat aacagacctc cgacagtgcc
     6661 agcagcagct ccaaataaca gtcctttgaa aaagttttta atcatcgcag gacacctgct
     6721 ttacgattgt ttcttgaata gcttttagtt cttccgatcc ataactatct tgatgattga
     6781 caaagtgaat agaaaaatca tcctctttgc tgtagcgagg gactaaatgg atatgtgaat
     6841 gaaaaacaga ttgataggct agttcacggt tgttattcaa gatattaaga cctttcattt
     6901 ctggaaaagc tttttccaat gcttgagcaa tcttaggcac acgcgaaaaa acctctcctg
     6961 caagttcggg atcatattca aaaatatctg ctacatgacg tttaggcaca actaatgtgt
     7021 gtcctttcgt tgcttgagag atgtctaaaa aagcatagac cttgtcgtct tcatatattt
     7081 tatagcttgg gatttcttga ttgatgattt tgcaaaaaat acagttttcc atataaaaca
     7141 tcctttctat aaagaataaa tacttcagct taactttacc ataagttaaa gctattgtaa
     7201 ggaaaaaaac aaaataccgt cattttcttc gtggtataat aaataaaaat aaatggcgga
     7261 ggaaaaagaa tgagtttaat tatcgatcat gtcacagggg ggtacggaca tctccctgtg
     7321 ttaaaagata tcagtttcga tgtacagcct ggagagatag tagggttgat tggactgaac
     7381 ggagcaggga aaagtacgac catcaagcac attatcggtc tgctggaagc ttcaaaagga
     7441 acgatagaaa tcaatcatct atccttaaag aaaaatacgg aagaatatcg caaacaaatt
     7501 gggtatattc cagagactcc ttctttatat gaagaattga cattgagaga acatatcgaa
     7561 gtcactgcga tggcctacga tatacctatg gatatagcaa tgaaaagagc agaaaagttg
     7621 ttggagactt ttcggttatc taatcgtctt gactggttcc cagctaattt ttcaaaggga
     7681 atgaaacaga aagtgatgat cttatgcgcc tttttgatcg agccttcttt atatatcatc
     7741 gacgaaccat ttttaggctt ggatccacta gctatcaatg cattgcttga attgatggta
     7801 gaaatgaaaa aagaaggagc cggtattttg atgtctacgc atattttagc tactgcagaa
     7861 aaatattgtg atcgttttgt ggtcttacat aacggagaga tacgagcaga cggttctctt
     7921 gagatgttga gaaaagaatt tcagctgccg gattcttctt tggacgaaat ctatattgct
     7981 ttaacaaagg agcaggcaca tgagtagttt ttttcaaaaa cgtttagcaa gacatcaaaa
     8041 gaaaatgatg aaatatatgc gttatgtatt gaacgatcat tttgttttag tttgtctttt
     8101 cttgattggc ggtgtgggat tatattattc taattggttg aaaaccctgc agccaccgtt
     8161 tgcaattgga gggctcataa tctctgtatt ttggatgctc tgtttattcg tgggtaagat
     8221 ggctactttt gcagaggcag cagatattgt ttttttactt cctaaagaaa aagaaatgca
     8281 aacctatttg gaacgagctt ttcgctattc ttgcattttt ccgttgatct ttttgatatt
     8341 ggcatgcgga ttggccatgc cattagtggt ggttagtacg gagcaatcct tttcgatgtt
     8401 tttcgtctat ctgatcatat tatggagttt gaaattcagt catctgcaaa tcaagagagc
     8461 agaattgttt cgactagatc aacaagttat tcgttattgg aaattaggct ggtttttttc
     8521 ttctctgatc tttttgtttc tatcatttta ttggctaata tgggcaggaa tagcaggtgc
     8581 attagtacag gcaagtctat attactggtt tttgtggaag caacaagaaa aaggattgga
     8641 ttgggagaaa atggtgcatg tagaagaaca taggttgcat cagatctatc aatttatcaa
     8701 tttattcaca gatgttcctg aaattacggc aaagatcaaa agaagacgct atttggatcc
     8761 tatattgact catattaaaa gagaatcaaa aaacacttat ctttacttgt ttgcaagaag
     8821 attggcaaga ggttctgatt ttagtggtct ttatttccga ttagtaatta taggtgggtt
     8881 attgattgcc ggagtagtcg acttttattt tagtctgatt atcggtttct tatttttata
     8941 tttgatcggt ttccaactgt tacctttagc gaaccagttc cgctatatgg ttctcgttca
     9001 gctttatcca atccataaga atcagaagaa gcaggcgatt cagtatctac tcacatgggt
     9061 attaattatc gcgtcggtta tttttggttt gattgctgca gtcgtattag atgggcagca
     9121 gcgatgggtt ccgctagcgg cttatttgat tgttactatg ctctttgtta aactatatgt
     9181 accaactcgt ttgaagaaga tgtcagactg aaaacaaagc tgtgtagggt aaaaatgaga
     9241 atgaaaaata gagcgttgac agtaagaatg ctgttcgttc tatttttttt gagaaaagct
     9301 ggttttttga atggcaatta aatgaaaaag aacgtatatg tgcttgacgt gtagtgttca
     9361 ggcgactaga ataataagta ctgcaaaaca aagagtaaga ggacgggaga atgcatgatg
     9421 gagtttcagt tagaccaaga gtggcggtta cgtcccatca aaggcgacac aggaaaaaca
     9481 tacataggca tgcgtgatga ggacaaagtc tttattaaac gaaacacgac tccgatgttg
     9541 gctgctcttt caagggaagg aattacacca aaacttgttt ggacaaaacg aacggggaat
     9601 ggcgatactt tgacagcaca agagtggctg gaaggtcgct tgctgactcc taatgagatt
     9661 ggtcaaagaa acgacgtgat tgatgttttg taccatttac atcattcaaa ttctcttaaa
     9721 accatgctga cccgtatcgg tggtcgtgtc atgacgccgg agaagatgct gcaaaaatat
     9781 gaaaaagaat taccggaaga actccagaaa aatcaatttc tgcaaattgt ccatcgtttt
     9841 cttcgtgagc agatcccgtc aatagataag gaaaacttat cagtagtaca cggagatgta
     9901 aaccatcgta attggttagt atgtaaaaat tatttatatc tagtcgattg ggattcaatc
     9961 atgtttgcag accctgcggt ggatattgca acgattttag gaaattatgt gccactgtct
    10021 agctggaatc agtggctgat cagttatggc atacgcccga ctgacgaaat gcttgaaaaa
    10081 tttcattggt atgcggccat gaatttgctc caagaaatca ccagatattg caaacgtgaa
    10141 gatcatcgac ggatgaacga agaaatttta caattaaaac gaattttcag tggctgaggt
    10201 ttggcccaaa ctctgcggag atttggccag acctttttaa ttagaggaaa ggaatggaga
    10261 taaataatgc gtgtccgaaa tcgtccggga gcagcggaaa tgttagcggc tcacccgaat
    10321 tttgtaatta gtgatccaac attgtggaag ggaaaatgga atgaattgtt tgaaaacgat
    10381 catccaatcc acattgagat tggtatgggg aaaggccagt tcatcactgg aatggcaaaa
    10441 gcccatcctg aaatcaatta tattggagta gaaatgcagg tcagtgttgt ttcgattgca
    10501 ctagacaagt tgatcgaaca accgcttcct aacctgaaac ttctacatgt ggatggatca
    10561 gcgttgacag agtatttcgc tgacagcgaa gtagatcaaa tctatctgaa tttttccgat
    10621 ccatggccaa aaaaacgtca tgaaaaacgg cgattgacat ataagacttt tcttgctgta
    10681 gatgaacaga ttcttcgtcc aaatggagaa atccatttca aaacagataa tcaaggattg
    10741 tttgagtatt ctctggcaag tttttctcaa tatggcatga tcctcaaaca ggtatggtta
    10801 gatttacatc aaagtcaatt tgaaggcaat attatgacgg agtatgaaga aaaattttct
    10861 tcaaaaggtc aacgtattta tcgagtagaa gctcaatttc aagataagac caaaaagaaa
    10921 agtctgtgaa actaactttc tgctttcata aaaacattaa tatccataaa taaatcccag
    10981 aaaatgaaca ttacagatgt aatgtatttt ctgggattta tttgatttaa aatgtattta
    11041 tttcatcggt ttttccacaa ggtggaatca attggaagcg agaaaatgaa aataatcctt
    11101 tgtcggtgat cttcaattcg ggtatgacag ataatgttaa gaaagaaagt gtcaaaaaag
    11161 gatcaaaggt tttagctgct cctagttgat gtgctttagt tattaattca ttcaactgtt
    11221 catagacgat cgaaaaaggt tcctgtgtca tgattccgcc tataggaaga gaaacacaag
    11281 ctatttcctg ctgtttgttg acaacggtca tcccgccacc tttttgaatc aaaagattag
    11341 cagcatacag catatcctcg tcgtttgtcc caacgacaac tagattatgc gagtcatggg
    11401 caatcgttgt cgctatcgct ccttgtttca gctgaaatcc tttcactatg cctaatccaa
    11461 tgtttcccgt ttgatggtga cgctcgatca ccgcaatctt taattggtct ttttcgattg
    11521 aaggaatgaa tcgttcgcct tgtcggtcca ctttttctac caaatcttta gtcactagac
    11581 tattcgggat aatttcgatc acatgggctt gtgaagaaga aagaagcagc tctaacgaat
    11641 gtggatttaa tggttgagcg tttagttttg gcagacaaca cgtatatgaa tttgaatgat
    11701 cagcaaataa atggtcttgg agttgcccag attgaacaac taatgttccc ttatagaaaa
    11761 cttcatgaat tggcagcttg gtaagatcgt ctgttaagaa gaaatcagct tgatacccag
    11821 gggcaatcgc acctagtgtt ttcaaatcaa aacattctgc tgcattcaat gttgccatct
    11881 gaatggcttg taatggatcg attccaagtg caatagcttg acggacgata tgatcgattg
    11941 atccttcttt tactaaatca ttgatcagtt tatcatcggt gacaaagaag catcttcttg
    12001 aattttcagg tgtgatcgcc ggtagtagcg atgccagatc ttttgcaacg gtcccttctc
    12061 gaaccattaa atacatacca agatccaaac gttctttagc ttcttttgca gttgtagctt
    12121 catgatccgt ccgaatacct gctgcaggat aaacatttaa ctcttccatg ccgataccag
    12181 ctgcatgccc gtcaattttt ttcttatgct ggtgcatcaa ttggatcttt tctaggatgt
    12241 ctgtttcatt atttgctact gcttgatagt tcatgacttc agctaagcct aaaacttcgg
    12301 gtttttgtaa aaaaggggct aatttttttg catttaaatt tgcaccattg gtctcaaaag
    12361 gggttacagg tacacaagaa ggtagcataa aaaagatatt catgagtgtt tgtcttgcat
    12421 cttctatcat aaactcaaga cctgtactcc cagcaatgtt tcctatttca tggggatctg
    12481 tcactacagt agtaactcca tgaagcaagg atacttttga aaattcgctt ggtgataaaa
    12541 gtgagctttc gatatgcaca tggccatcga tgaatcctgg cactagatat tgcccattag
    12601 cattatgtac gctttttcct tcgtattcac cgattcctac gatcattcct tcgcagattg
    12661 ctacatcttt ttccatgatt tctttcgtaa agacgttgac gatcttagca tgcttgatca
    12721 ctaggtcagc aggctttttt ttacttgcta cttcgatata tttccttaat gttgtttcca
    12781 atggagtgcc tcccaagata aatttttata agtataacat aaattcggtt tttgccaata
    12841 tttttttgta aattcatttt atttgaaaca aaaagatcaa aaagacgtat gttttttgtg
    12901 gaggggagaa taatggaaat aaaacgatta tcggatcaaa tgataagtga aagttttgcc
    12961 aaacatgccc aagacgtata tatattgcca gcttcagaag tattttacat ctattttcgc
    13021 tatggaaaaa aacgaacatt ctggaagaaa gtctcttcgg cagatggaca gcaactgatc
    13081 tcaagattta agtatttggg acagatggat atcggagaaa aaagaaaagc tcagttaggt
    13141 gcatttactt attctttaga aaaacaggaa gttcgtctgc ggttgtcttc tgtaggagat
    13201 taccttcaac gagagagttt agtgattcgg ctattatacg gtacttttga aacaccccat
    13261 cgttgcttta gttctcgaga ttttgcaact attttacaga aaaccgtcag acgtggatta
    13321 taccttttca gcggaccagt cggatcaggc aagacttctc taatgtatcg gttggctttg
    13381 gaagaaaaat tgcaagtgat tactatcgaa gatcctgtag agatagaaga accgtttttt
    13441 ctgcagctac agatcaatcc gaaaatagca cagacttatg atacactttt gagactcgca
    13501 ttacggcatc gtccagactt gctaatcatt ggcgagataa gagatgaatc aactgcacat
    13561 gctgcggtaa gagcagcact aacagggcac cgggtttttg caaccgtaca tgcacgcaat
    13621 cttgaaggta cattggtcag aatccatgaa ctgacaaaga aaaaagaaga gctatctgaa
    13681 tgtttagctg gtgtcattta tcaagaactt ttggaagatg ccaatcacca tccttctgtt
    13741 ttatggagtt atcaatttat ggaggaagaa tcagtaaaaa aatcatggga ggaaagttat
    13801 gaagaagctc aggaaaatat ttggcagtca gaactattct aaaggattaa caagaagcca
    13861 gcaaaaccag tttgcacaaa ccatgggaga actgctttta tctggtttta gtctgcaaga
    13921 atccctttat ttattactca cgatccgttc gtttccacct gttattttgg caaatatcca
    13981 acatcagcta aaggaagggc aagcgtttag tgaggtactt tcgcaaatag gatatggagc
    14041 agagcaactg gtacagatcg agttagctga acgtcatggg aatctgccga caactttgtt
    14101 ggaaattgcc aaacagtttc ggcttgttga aggatttcgc aatgatttga aaaagctgct
    14161 tgcatatccg atgctattgc tcaccttttt gctaggtatc ttgtttagta tgcggttgtt
    14221 cgttttgcct cagcttattg gttcaggaat ggtcatgcaa gagcattggg gtattcagct
    14281 gatacaagtc tttcattggt atgttctact attcatccta gcggttactc tattggcttt
    14341 actagtaagg tatcgtctga tgaagttgtc tcctgttgat cagagtgaat ggataagcag
    14401 acaattattg attggaccga tatattcctt gtatcaatcg gcttatttgt cactagagat
    14461 tggaaagctt tttcatgaag gtttcgaatt aaggcaaatc atagtctgct taaaggaaac
    14521 gaaacaagaa agtctgattc agcatcttgc ctctaagttg attcaaggat tggaggcagg
    14581 tatgtcattg gctgagcagt tccagaatta tcgttttcta tcagaagaat tcagtaaaat
    14641 tgttttacag ggagaggcaa agggaagttt aggtaaagaa ctgatttttt acagcgaatg
    14701 gacaagaaaa cagcttttca aacgtcttca tcactggatg cattttattc agccaatcat
    14761 ttttttgggc gtagcggctt tgattttatt agtttatgcc gctgttttat tacctgttta
    14821 cggaagcata gaggaggttt tgccatgaaa aagagaaaaa tatcttatta tagcggtttt
    14881 acattgatcg aaatgctaat cgtcttactg atcatcagtg tattagtgtt gttgttcgtc
    14941 cctaatttgt cgcgttatag gaatcatgta gaccaagaaa gtcgtgaagc gatcatccag
    15001 ttggtagaca cacaaaaaga gctctatgcg cttcaaaata atggaagagt tcctactgtg
    15061 gaggaattgt tgaatgaagg ctatatcaaa cgagaacatg cagaaatcta tcagcggcct
    15121 tgaaggtaag gttttagcat caggatatac gttgtttgaa tcactcttac tgattttatt
    15181 acttacaacg atcctttctt ttccagttct cgcattttct gcatggaaga atgaattggc
    15241 agtctatcaa tttttcggcc aatttgaaaa acgaatctat tctacgcaaa aaatcgctat
    15301 tgtgaatcag atccaaacag gcttttattg gaatagtgaa gagaatcaaa tcatttttca
    15361 tgttccgaat ccagataaaa taaattggga agtattagat attcctgatg agattacctt
    15421 gaaacgccat gcttctatta cttttgctgc tagaaccggg aatgaaagtt cactaaaagc
    15481 ttatcaattt tattgggaag aaaaagagcg gacgattacg tatcaattcc agatagggag
    15541 tggtcgctat acaaaaagaa ttgaataaaa caggatttgt tttgttggag tcccttacag
    15601 cgcttatgat cgcttctgtc tgtgtctact tagtgagctt tggccagctg tttttgcttc
    15661 aggcagaaaa gaaaagtggg gaggaactgc tatttattcg aatgatttat gaagatatca
    15721 agacgcagcg gatgtatcac aaaagaccag agcgaaggga agaaatgatc ccatacgata
    15781 tccaaattgt cgataataat ggagtaatcg aaaaagtgtt tgtcaatatg gagaaagaag
    15841 cgatcagtat tgaaaaaaca agtaattcca gcatttacgc tgattgaatg tctaattgga
    15901 ctgatgatat tgagcagtct attgctcaca ttcaatcttt tgattcagca aaccatccaa
    15961 gtaagtgaat ttttgcagcg taaagatcaa aaagagtggc taatattttt agcacaatta
    16021 gaagaagaac tcaagagcag tcacaatgtc tctgtgaaaa atcagcaact gcactacacg
    16081 atcgaggata aacagtatat catcgaaaga tatcaggcaa tgatacgtaa aagaagaact
    16141 tccggcggac atcaacctat gttaacaagt atcactgagc ttcaattatt ggaaaaagaa
    16201 cacacaattt ctttttatgt acactttgaa aatggagaag atggatatgc catatggaca
    16261 aaaaacgatc agtagaaaat gttataaggg ctctatttta ttttctgtct tgcttttatt
    16321 tcttttatgt acgtttttat tgatggcagc attagaaagc taccgtttgt caatggatct
    16381 taatatacga atcaaaaatt attatatagc aaaaataatg aagcagatgg ctttatcgga
    16441 acagtctacg cttgccgaaa gttcggaagg agttttttac tacacaactg gatctgtaac
    16501 atatcagtgg gtacaacagt ccctattctt agaggtggaa gtctcaccgt ttatctttcg
    16561 gttcacagaa gaagtaaaaa atgatacaga tactagtaca gaatgatgga atgttcgtga
    16621 aaacgccctc ttttctgtta taacactgtt tttaataaaa aaatgtttgc cgtttcacaa
    16681 ataaaaaaca gtgaaaacga ttgaaatttt gtcaattgtt cgatagaatt aaacaggtga
    16741 aaaaattgtt ataatatatc tagaaatgag gtgcaccatg ttaccagaaa aaatggaaca
    16801 agggtttaat caaaacctgg aagctattca gcttttacag aatgcattgg gaacttcctt
    16861 tttagaagcc tatgtcgaaa atgcagaaaa tttaatagat gattatcaag tacgtgtggt
    16921 ggatggagtg ccgacaaaag agacaacgca acggatcaca gctctttatg aagagttgaa
    16981 gaaactttct ttcgagccag aagaatggcg cagactgtct caattacttt tattgaaggg
    17041 cagccagaca gaacatttgc aggcgaacca tcaactcact cctgatagta tcggtttttt
    17101 atttgttttt cttattgaac aactatacac aaataaaaaa gcaccggtta agattctgga
    17161 tattgcggca ggaatgggca acttgttatt aacagttctt ttaaacctat cgaatgcggg
    17221 gtaccaaaca gaaggaattg gtgtggatat tgatgataca ttgttggctg tcgcagcttc
    17281 tacaagtgat ttgacacaag caaatgttca atactttcat caagacgggt tgcaggaatt
    17341 attaatagat cctgtcgatt ttgcaatcag cgatttgcca atcggttatt atccgaatga
    17401 cgaaaaagca aaagagtttt taacaagtac cgaagaaggc catagttatg cacatcattt
    17461 attgctagag caatcaatga aatatgtgaa gccggatggt tttggtttgt ttttgatgcc
    17521 gagtggattt ttagaaacgg atcaaagtga agaaatcaaa aaatggttta aagaagaagg
    17581 ttatcttcaa ggaatgattc aattgccaga tgaattgttc cgtaataaac aatcgcaaaa
    17641 aagtattttg atcttacaga aaaaaggacc acaagcacaa caagtaaaag aagtcttgct
    17701 tgtgaaactt gcttcattaa aagaaccaga gaaggtgacg gaatttttca acgagtttaa
    17761 aaactggaaa tcatctgctc tgtaatcatt caacgagaag gagagtcata tgtctaaaac
    17821 aattgcaatc aatgctggga gttcaagttt gaaatggcaa ttatatcaaa tgccagaaga
    17881 aaccgtagtt gcgaaaggga tcgtcgaacg tatcggttta caagattcta tttttaccat
    17941 caagtacggc gataatcaaa aattcgaaca aatcatggat atcgaaaacc acgaagtagc
    18001 agtaaaaatg ctgttagatc agttgattga actgcaaatt ttaagctctt atgatgaaat
    18061 caccggagta gggcaccgtg ttgttcacgg aggagaaact tatggggatt cagtagtgat
    18121 cgatgagaaa gtaatggacc gcattgctga attagcagaa tttgctcctt tgcataatcc
    18181 tgcaaactta atggggatca aagcgtttaa aaagatttta cctgacattt tgagtgtggc
    18241 tgtattcgat acatctttcc actcaacaat gccaaagcaa aattatttat atagcttacc
    18301 ggtgtcttat tatgaagatt tcaaagtaag aaaatatggc ttccatggca caagtcatcg
    18361 ttatgtgtcg gaacgtgcgg caaaattatt gaacaaaccg atcgaagaac taaaaatcat
    18421 cacttgccac ttaggaaatg gtgtatcgat cacagcagtc gatggaggta aatccatgga
    18481 tacttctatg ggattcacac cattagctgg ggtgacaatg ggtacacgtt caggtgatat
    18541 cgatccagct gttctcccat atttaatgga aaaattagag attgatattg atgaaatgat
    18601 caatgttttg aacaagaaat ctggtctgct aggcctaaca ggattgtcta gtgatatgcg
    18661 tgatcttgaa aagaactatg aaaaagaaca tattcgttta gcttatgaca ttttcgtgga
    18721 ccgcatccga aaatacatcg gcggttatgt gacaacgatg aatggtgttg atgcaattgt
    18781 ctttactgct ggtatcggcg agaatgacgg acatgtacgt agtgaaatca tcaaaggtat
    18841 gtcttggttt ggttgtgaaa tcgatccagc gttgaatgaa ttgcgtggtg aagaagtaga
    18901 tatctctaca aaagaatcaa aagtaagagt attagtgatc ccaacagatg aggaactaat
    18961 gattgcccgt gatgtggagc gtctgcgcgg ctaagccaaa gcattcgtta ggataaaaga
    19021 tgaaacgcat gcaagaggga attcttgcat gtgttttttt tgcggagaag accgcaaaat
    19081 ggtaatttaa cttcaatttc ccttcttttt tacaggatct tcttagggaa ttttgcttgg
    19141 aaaagtggtc aaaagggaaa agactggcat tttatttatt gattttcagg aaaatcccaa
    19201 tattttattt tcattcatct ttataaggga cattacatat gtaaaaagaa aaaacatttt
    19261 tagatcagcc tcgtgggctg tcgactttat tcttcgcttt atgtttagct agaaaaccga
    19321 tccgaaacat tcattgatcg aataataaaa acttgatttt ccacctgtac tttgcctgaa
    19381 tcgtgaaagc acaggtatga aaatcaagtt ttttgttgcg aaaaaacgtc ctagtcaatc
    19441 agttggccga gcggctggcg gctgatctgc tcctgatcaa taacgagtgc ttcttggaag
    19501 tgttgctgca aaaagtagcg gtcatggctg actgctagga gcgttccttg atagtcttgc
    19561 agcatttctt caatttcttc tcgagcatag ctgtcgaggt gattggttgg ttcatccaaa
    19621 atcaggacgt tgattttttc taaaaagagt ttcagcaacg ccaaacggat tttttcacca
    19681 ccggataaat ctttgatccg tttacccaca tcgctttgat aaaagccact tttggctaat
    19741 tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg cgtaagccag caaacggcta
    19801 tcgggatcgg caaactgcag ctgctgcggc aaataaccga tttgcaagct actacctaat
    19861 ttgatggtgc caccgtaagg cttttcctca ccgagtaata atttgagtaa ggttgatttt
    19921 cctgcaccat tgtcgccgat gatggctaaa tgatctccac gatagactgc aaaactgctg
    19981 tctttgaata gtacttgatc agcgtactgc tggtgcaagt tttgaatgat gaaaacttct
    20041 ttgccagatt ttccaccatt agacaagctt tgcagccgat ttttcggggg aggtggcggt
    20101 ttgacgagtg tcagtttcgc caggcggcgt tccagttctt tggctttttt gaagaaggac
    20161 tcattgtctc cttcatgtgc ccactgacga aaacgacgaa tcatgacttt caaccgctgg
    20221 acttcttttt gctgcagttc gtaatttttt gtcagctcag ccaagcgagc ttctttcaag
    20281 actcgaaaac ggctgtagtt tccgggatac tcaatcaatg caccttcttc cagttcccat
    20341 gtttttgtgg tgacttgatc gagaaattgc cgatcatgag aaacgaccag aaccgatttt
    20401 tttgtattct gtaaatagtt ttccaaccag cgaatactgt taagatctaa atgattggtc
    20461 ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa ttttggctaa agcgacttta
    20521 actcgttcgc ctccgctaag ttctgttaac cgtgcttcct gacggtgtgg aatccctaag
    20581 ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac cgcctagttc ttcaaagcga
    20641 tgctgcagct caccgtaacg ggccagacac ttctctaagt ttgtatctgg ttctgtcatc
    20701 aatgcttcgt agcgactcat ttgctgtttg aggtcctgta tctcagcggc agaatggagc
    20761 aagtagtggt tgacggtcgc ttcggaaact gtaagttctt gttcgaccgt cccgatcttt
    20821 aagccttttt tgcgactgat aacaccgctg tcgacacctt ctctgccggt caagatttgt
    20881 aaaaaggttg atttgccagt tccgttgata ccgactaagg caattttttc accagcttca
    20941 atttgacagg ataaatcctc aaataatgga ttcccgccga ttgttttttt aatagaaatt
    21001 gcttgtatga tcatgatctc tctccttgag accactcaaa aaggccatgg ttatccatgg
    21061 cctgtgttat ttaaactcag attatcatga gaaaaatacc ttgagatggc cattgatttt
    21121 acgtgaattc aactgttttg gacaaacttg tcccgtcaac agcaactgta aaaccaattg
    21181 catggctaaa tgcgatatgg tatcgttggt aattttcatt acttgttatt ttagtcatgc
    21241 gcatcatagt attttccctc cttcgtgtag ttgtcttttc cagtgtacct gaaagtgtca
    21301 gaaagttcaa ctagcgctga catttactta atatttatcg atgtgtaatt ctgtcagaga
    21361 gcttttttta tctgactgag ggtaatgtct cgtggaccac atcattgtat tctgagctaa
    21421 accagcgttc aggcgaaatc atatggtgtt tgataaaaaa ctttgtctac atgacttcct
    21481 tttgccattt gcttaatacc gttgcattaa acagctcttc tctttggaaa tctcaccaac
    21541 atcgcgttca ggagtatcgt tgtggttgca atggcaagcg gtagcctttc atcaattctt
    21601 gaatgattta ccgaatgtag cacttgcctt ctcgcggtac ccgaacccga aataaaattt
    21661 tagtttggcg ataaaatcgg tatccgccat cgatacttca tattttttcg tatcagattc
    21721 atcggaaact tccacattga cgaaccaata agctttggca tgcttaggtc gttttaaacc
    21781 acaccagggc gactaatcgc cctggtgttc actttttttt attttacttg aactcattga
    21841 ctgttttccc tttattttaa acattataaa gaccgatgag tgcttgccgc gtcttttgtt
    21901 tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta
    21961 agctttggtg tggtctttag ctggcaattg gttttctaaa tcagttcgga caacaagcac
    22021 atcgcaagct gcgtttctaa taacatattc agaaacagaa ccaataaata aacgttctac
    22081 agcattcaat cctgtggctc ctaacataat caaatctact tgattatctt ctgggatttc
    22141 tctggcaata atttgttttg gtgagccata ttctacgaca gaggttacat tattcaagcc
    22201 agctttcttc gcattgcttt cataatctgc caatgtttgc tttgccattt cagtagcttg
    22261 ttctgcaagc attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg
    22321 tgctaataga agttctccgt tatttcggat agctacattg accgcctttt taaaagctaa
    22381 ttcagcttca tcagaaccat ctacggcaac catgattttt ttgtattgtt gtaacataag
    22441 cattcactcc tcaagtcaat cgtattgtac tcttttattg tagaacactt tagggaaaaa
    22501 gagaattatt tactgctttg gttcagcaat tttttttgca aatcggatac tgaaggtggc
    22561 agaaacaata attacgatca gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc
    22621 tgttaaaata cccattacgc ctaaaacagc atatgcagct ccgtaaaatt gtaggaatcc
    22681 ttgattttta tcatttttgt tgatcaatac aaaaaagata tcctgtttat taaataaata
    22741 atagctaacg aataataata ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa
    22801 atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat tgctttattc
    22861 tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa
    22921 ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc aaatgataag gcatgttacc
    22981 agcagtgaca caagacgggt cccaagatat caataaaaat gttcggtcaa cacacaaaga
    23041 ggacaattcg aacatcacag atttcccacc tctatagtag catatgaatt taaaaagagc
    23101 aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga ggatgctttt
    23161 tccactcttt tatacggtaa tactgttgac ccagagcctg ttcatatttc cctgtatctt
    23221 ttggctgata atactgagca ttcttcagtt tatctggtaa atattgctga ttgacccaag
    23281 cctgatcaaa atgatgagga tactgataac ccacgccacg gttgagtgat ttcgcacctt
    23341 tgtaatggct atctctcaaa tgatcaggaa catctccagc tttgccctct cgaatatcag
    23401 caagtgctgc atctaatgcc atgtaggcag aattggattt tggcgacaag caaagatcga
    23461 cgacgacgtc tgctagtggg atacgtgcct caggcaatcc caatttttca gcagctaata
    23521 cagcgttcac tgtacgagcc gcagcagcag gattacctaa acctatatct tcataaccga
    23581 taaccatcag acgccggcaa atacttgcca gatcacctgc ctcaactaaa cgggcgagat
    23641 aatgaagtgc agcatccaca tcacttccac gaatcgattt ttgaaaagcc gaaataacgt
    23701 cataatgagc atctccattt ttgtcatggg taagagcttt tctttggaca cattcttcaa
    23761 tgatcgacag tgtaagatga atccttcctt ctttatctga aggagtcgat agtgtagcca
    23821 gttccagccc attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa
    23881 gagcatcttc gtctaactgg attgcttgtt gtccaaggcc gcgttcttta tcccttaaag
    23941 catgttcgac tgccaactgg atgtcttgtt ctgttaatgg tttgacttca aagatctgtg
    24001 tacggctgcg gatcgctgga ttgatggtaa tataaggatt ctcagtagtc gctccgatca
    24061 agatgattcg gccactttct aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc
    24121 ggtgtacttc atccaataag aggatcactg taccactcat ttttgcttct tccgctacaa
    24181 cttgtagatc cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca taattagtag
    24241 agccggcgat cgcacttgcg atactcgttt ttccagtacc aggaggtcca tatagaatca
    24301 tggaagagag catgcgagca tcgaccatgc ggcgtatgat ttttccttcc ccgactagat
    24361 gctgctgacc aacgacttca tcgatcgttc gaggtctcat gcgataagcc aatggctgtt
    24421 gcataggatt ttcctccttc ttgtttttac gtcccttaat tatatcatat ttagcaagat
    24481 gcctagaaag aacagccttc tgttgttttg ttttcatagc ttatccaact aatattgtgt
    24541 atgataaagt aggtgagaaa aatgacggat aaacaaaaga tagatgaatt tttttggaat
    24601 aaaacgacgc cagaagtggc acgggattta ttggggatgt atttggagca tgacacacca
    24661 gaaggaagat tagctggata tattgtagac gcggaagcat acctaggtcc agaagacgag
    24721 gccgcccata gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca
    24781 ggtacgatct acctttacac gatgcatact catcggattt tgaacatcat tactcagcca
    24841 gaagggattc ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa
    24901 atgagtaaaa atcgtggcgg aaaaactggt ccagatatca gcaacggtcc gggaaaatta
    24961 gtggaagcat tagcaattcc tcaagaactt tatggtcagt ctatagcaga tagttcgtta
    25021 cgcttagttt ttgagaaaaa aaagacacca aaaaagatca atgctttgcc tagaatcggt
    25081 atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct
    25141 tatatctctt tacaaagaaa aaaccaaatc gagaaagatt ggggttggag gaaagaaaat
    25201 gaaaaagaag gatctattaa catatttaga tgaaataatt gaaaaacaag tgacagacta
    25261 tgatgtagct ttagattggg atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt
    25321 tgcagaaaat aaagcacaat ttgagattga tgatgcagaa gggatcgttt cgcaagaacc
    25381 aattatcgaa tttgaagacg gcgtacttct ttttaatcct cagaaatctg tttttgatga
    25441 acgcaattat ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa aatcagtagc
    25501 tgattcttta gtggaatatc taaatgaagt attagctcaa gggcaaagcg atttgcttga
    25561 ttttttggat gaagacgatg acgaagctgt attcgagctt cactgggata atcagcaact
    25621 agcagaatta gtagaagtaa aacagcaaaa tgaagcagat acttatttac cgtacccaag
    25681 ttattaagga gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc
    25741 agtcgaagca gttgctaata ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga
    25801 ttctttagat gttgaacatt ttcagtcgga tcaatatggt gagattttag ataaaaatga
    25861 ttttacatcg ctgaaagaag gcgcactagt catggcttat tttccggaaa cgactttctt
    25921 accggaaatc ttgcctttta tcaaagatcg tatcacacaa ttacctgagt ttggtctagc
    25981 tattggaaaa aataaagtaa cagttagtga agtagaggaa agcaactggg caacagcctg
    26041 gaagaaatac tatcatccgg tgcgtattac gcgaatgctg acggttgcgc caagctggga
    26101 aaactaccag acagaagatc ctctagaaaa aattatcaca ctagatcctg gtatggcatt
    26161 tggcacaggc actcatccca cgacttcctt gacattgaca gcactagaaa cagtcttgcg
    26221 tggtggtgaa acattgctag atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa
    26281 atatcttggt gcaaaagatg tctatgcctt tgatttagac gaagtagccg ttcgttcggc
    26341 gaaagaaaat atggatatga atgaaatagc caaagatgtc catgtatcag ctaacgatct
    26401 tttgacagga atcgagattg aaagtgatgt aatcgttgcg aacattttag cagatatcat
    26461 cttattgatg atcccagatg cttggcgttt actgaagcaa acaggcacgc tgatcgtttc
    26521 aggaatcatt gaagagaaga agcaaatggt tctggatgcc atgttagaac aagggtttat
    26581 cgtggatcaa attttccagc aaaaagactg gtatgcaatt attttgaaaa aaccagaggt
    26641 ggaatagatg cagcggtatt ttttaaaaga atcatatgaa tcaaaagact attttgtttt
    26701 agaaggggaa aactatcacc acattgtacg ggttatgagg atggaacccg aggatgaagt
    26761 ttttttggca ttcaatgatc aaatagccat tgttgcagaa atcacggaga ttactgaaga
    26821 ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat
    26881 taccatagcg agtggctatc ctaaagggga aaagctggat tggatcgttc agaaatccac
    26941 tgaattagga gcgcaagctt ttcttggttt cccggcatca gcctctgtcg ttaaatggga
    27001 tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag attgctcaag aggcagcgga
    27061 acaatcccat cggcagatcg tgccatcagt agagttattg gaaacgaaac aagatttatt
    27121 tgagcggcta gatgattatg acgtggtctt ggttgcttat gaggaatcag caaagcaagg
    27181 cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa ccagatagtc gaatcttagc
    27241 tgtctttgga ccagaaggtg gattatcccg cgatgaagtc gattattttg tagaacacaa
    27301 agggatactt tgtggattag gaccaagaat ccttcgtaca gaaacagcac ctctttattt
    27361 attaagtgct gctagctatc aatgggaatt aatgaatcct tctttcagct aacgttcttc
    27421 attaataaaa gagtatcgac agaaagaatt gaaaaaatag tcaacaattt gtataataga
    27481 aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca
    27541 tttccgtaaa aaaacgtata actgcacgga ttttgggaca attatcaaac agatatttgt
    27601 cccgattccc gttttaccga aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa
    27661 ttttaacggg acccggtgtc ataaagctcg ttagtcaata catgggtccc gaacatgtag
    27721 catttgttga gaaagcatgc gaatacgcga ctgctgcaca tgacggacag tttagaaaat
    27781 caggcgaacc ctacattatt catcctatcc aagtcgcagg tatattagca gatttaaaaa
    27841 tggatcccca tacagtggct acaggcttct tacatgatgt tgttgaagat acagaaatca
    27901 ctttagaaga tctgagagaa gaatttggcg atgacgttgc tatgttagtg gacggcgtaa
    27961 ccaaattagg gaaaataaaa tataaatccc acgaagagca gctagcagaa aaccaccgaa
    28021 agatgctgct agcaatggct caagatttac gagttatcat ggtcaaatta gctgatagac
    28081 tacataacat gcgtacgttg aagcacctgc gagaagataa gcaaagaagg atcgctcagg
    28141 aaactttaga aatctatgcg cctcttgcac atcgtctagg gatcagccgg atcaaatggg
    28201 aattagaaga tacagcactt cgttatctaa atccaaaaca gtattaccgt atcgtccact
    28261 tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt gaagatattc
    28321 gaatagcgac agaagagctg gggatttttg cagaaatcta tggacggcca aaacacattt
    28381 attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt tatgacttgc
    28441 tagctattcg agtgatcgta gattcaatca aggattgtta tgctgtatta ggagcaatcc
    28501 acacaaaatg gaaaccaatg cccggcagat ttaaggatta tattgccatg ccaaaagcta
    28561 atatgtacca atctttgcat actaccgtga tcggtccggc aggaaatccg gtagaaattc
    28621 aaatccgaac acaggaaatg catgaaatcg ctgaattcgg ggttgctgca cactgggcct
    28681 ataaagaagg aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa ttaagctggt
    28741 tccatgagat actcgaactt caagacgaaa gctatgatgc ttctgaattt atggaaggcg
    28801 taaaaggaga tatctttagt gataaagtct acgtcttcac accaaaagga gacgttactg
    28861 agttaccaaa aggatccgga ccattggact ttgcatacag tatccataca gatatcggta
    28921 acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca gcttgattac aaattgaaaa
    28981 acggagatat cattgagatc atgacttctc caaattcatt tggcccaagt cgcgactggt
    29041 tgaaattagt tgctactagc aaagcaagaa ataagatcaa acgtttcttc aaagcccaag
    29101 atcgagaaga aaatgtgatc aaaggccacg aatccgtggt caaatgtatt acagatctag
    29161 gatttacgcc taaagatatt ttgacgaaga acaaactgca agaagcactc gatcgtttta
    29221 attatcaaac agaagatgat ctctatgcag ctgtagggta tggagaagtt agccccttga
    29281 cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc
    29341 aagaagcaga agaaatcatg aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc
    29401 atgaaggtgg cgttgtcatt caaggggtag aaaacttact aattcgtatc agtcgctgct
    29461 gtaatccgat tcctggtgat gatatcgttg gttatatcac taaaggcaga gggatatcca
    29521 ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc aaatgtagca gaacgtttga
    29581 ttgaagtcga atgggaagat acatcgaata cacgaaaaga gtatgatgca gatttggaaa
    29641 tttacggcta taatcgttca ggcttattga atgatgtact tcaaacagtc aatgcgctaa
    29701 cgaaaaatct caacagcgtt gaagcacgga cgaataaaga taaaatggcg acgatccatt
    29761 tgacggttgg tatccagaat ttatcccacc taaagagtat cgtggataaa atcaaagcag
    29821 tacctgatgt ctacagtgta cgccggacga atggatagga ggacagatat gagagcagtt
    29881 gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc
    29941 tacggcctac ttgttttatt aggcattcat gaaaccgata cgcaaaaaga tgtggactat
    30001 ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt
    30061 agtgtagaag atgtgggagg acaaatttta agtatctctc aattcacact gtttgcggat
    30121 acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac gaccagaaac agctattcct
    30181 ttatacgaat cattcaatga aggaatcaga agtcgcggca tcacagtcgc taccggacaa
    30241 ttcggagcag atatgtcggt ttctctcgtc aatgatggac ctgtaacgat cattattgat
    30301 tctaaaaaca aataaagtaa tctggttttc actagtaaaa actatgaaac acgtcgctta
    30361 agttagactg ataaactcta acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg
    30421 tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt tattttttat aggaggttgc
    30481 tgaagatgaa agaagtacca gtagtagata tcaaaatcac agatttaaaa aaattgtatc
    30541 aaaaaggata caacatgttg gaggagttac gccatgaagc ggacgctccc gtcgtcaaag
    30601 caaagatttt caataaagaa gctatcacga tctacggatc atcagcagca aaagtatttt
    30661 atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg aagacacttt
    30721 ttggtcaagg tggagtacag actttggatg gagctgctca tcaccatcgg aaaaatattt
    30781 ttatggatct aatgacaccg gaaagaatgg aagattatca tcgtatctta gataagaatc
    30841 tgacacaagc acttgaagca cagcatggtc aatttgaact atttgattta agtaagatgg
    30901 ttttctttac atctatttgc gagtgggcag gtatcaattt atcagcgatt tctaaagatg
    30961 aggtcgaaaa attagcagaa tatcaaatct ctatgatcag cggaacgttt acttctccga
    31021 ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga aaaatgggcg caaggcttga
    31081 ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg
    31141 ccaatgccac agatttagat ggtcagcttt tacctctgga agtggcagct gttgaattat
    31201 tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac
    31261 ttttcagtag accagatttg tatcagcaat taaaaaatga cttttcgacg ttacaagatc
    31321 cgtttattca agaaatgaga cgctattatc ctttcttccc aatgctacct gcgatttcgc
    31381 tgaaagaagt agaagtagat ggttatcgta taccagaagg cagttgggtt attttagatt
    31441 tatatggaac agaccatgat gaacgaacag ttgaagcgcc agattccttt atgatcaaac
    31501 ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga atatgagatg attgcacaag
    31561 gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga atggatcact cttcatagtc
    31621 tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt ctctgtacct gaacaagatt
    31681 ggacgattcc attcaatcag tttccgactt atccgaatag ccgagcgcta ctttacaaga
    31741 attagattag cataaactta tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt
    31801 ttcatattac tataaattga taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat
    31861 ggtttgataa tcttcattgg ataaatgcaa atctaaagct tggacattgt cagatacttg
    31921 ctctggacgc ttagctccag gtataactac agacactcgt ggattcttca tgtaccaagc
    31981 taaaacaagt tgcgcaggag tggcttggta tcttttagca atggggcgaa gtacatcgac
    32041 agcggttaaa atttctctga aacgttctcc ttggaaatct ggatttcttt ttcgaggatc
    32101 tccttcacca aattgttttt cctcaccaag ttcatatttt cctgtcagca aaccggaagc
    32161 aagaggaaaa tagggaacaa aactgatttt gtttttttca agataaggga ataattcttt
    32221 ttcagcctgc cgatggatca aactgtattt atcttctact acatcgacat atccatctgc
    32281 attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc
    32341 tgcttctttt aattcgtgca atgtagcaac tgattcattt tttggtgtgc tttcatccgg
    32401 aaagtggata taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac
    32461 cgattgtttg agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc
    32521 agcttttgta gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc
    32581 aatcagttct tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc
    32641 gctattcaaa gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag
    32701 attatgtcct ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg
    32761 tccgattctt atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac
    32821 caaaaagctg aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga
    32881 gactggtttt tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt
    32941 tgaaacttta caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa
    33001 agacactctg gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag
    33061 gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca
    33121 tgatgaacaa gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt
    33181 ttccaaagac caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt
    33241 gtcgaagaaa cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg
    33301 atttttgaac actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca
    33361 aaagagatgt atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt
    33421 acagcaccaa tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa
    33481 ccgttcaaaa ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg
    33541 ttacgtcaat tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat
    33601 gtggagagca tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg
    33661 ctagtcatca attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc
    33721 gactatcttt taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa
    33781 aatccattac gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct
    33841 ccatctatct tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc
    33901 atgctggatg cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat
    33961 tattacacac atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca
    34021 acgatttgcg caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca
    34081 cctggcttcg gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa
    34141 gtagttatcc ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca
    34201 aatatcgaag cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc
    34261 gacttcatga accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa
    34321 ttagtattgg tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg
    34381 gcaagccgaa aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc
    34441 tatgatgaaa tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct
    34501 attgcggatt agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac
    34561 aaaaaagacg agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg
    34621 ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc
    34681 gcagtgaata cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa
    34741 atcctaaaat ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc
    34801 aagcaaaaac cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt
    34861 tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa
    34921 atgcgacaac aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa
    34981 caccttacct agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg
    35041 tccatgctgg acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga
    35101 tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac
    35161 gtggagaccg tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag
    35221 aagaaatcca aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta
    35281 tcgaagtcac attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca
    35341 gcgacaaacc ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag
    35401 aagtagaatt caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga
    35461 atgctaaagg cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg
    35521 taggacaatt cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag
    35581 gtccaattgc taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa
    35641 aaccaggtga cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag
    35701 gtgctatccg gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt
    35761 tcttatgggt aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct
    35821 ctgctcacca tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc
    35881 ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ttaggcggag
    35941 gttctatccg tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta
    36001 caaaagaaca aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc
    36061 ctccacatgg cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag
    36121 ataatattcg tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg
    36181 atgcaccaag tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata
    36241 tagaagaata gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc
    36301 ttgatttaat tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca
    36361 tgaaaaaaac attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta
    36421 tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt
    36481 attcaagtgg tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag
    36541 gattcgaagt accggtatca acaactttgt atttactgaa tatacctttg tttgtattgg
    36601 cgtggttaaa aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct
    36661 ccctttgtat ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt
    36721 tgtttggggg ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat
    36781 cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt
    36841 ctatttcgat ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc
    36901 aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt
    36961 atactaaaca aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa
    37021 atgaaatcca aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt
    37081 ataagcatga taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac
    37141 ttagagaagc aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc
    37201 agatacttgg tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa
    37261 atactgctgt agacagacgt tcgtttgtct gcagtttttt tctaccatag tagaatcagc
    37321 aaagaaaaac tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc atgtttattt
    37381 ttttaaattt atgggatgat agaaaaaaat agtagagaaa ggacacgatt ctacttgcta
    37441 ttttcactta atacccgtat attacgaatg aaaaaagttt atgaaaagtt ggaatattga
    37501 atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga tgggctccgt
    37561 tcattagctg tggtgggtgt tatcttctat caccttctgc caactagtat gaaaggcggc
    37621 tatttgggcg ttccgatttt tttcgtcgta tctggttatt tgatcacgga tctgctgcgg
    37681 caggaatggg aacaaaatgg aaaaatcaat atttggcaat tttatgtcag aagaatgaag
    37741 agactctatc cggcacttgt ttttatgctt gtgacagctt cggcttatat cactttgttt
    37801 cagcgagggc tgttaaacaa tcttcgagga gttgtggtca gcagcttact gtatgtcaac
    37861 aactggtggc aaatcaataa cggtttatct tatttcgatc gatttgctaa tgagtcaccg
    37921 tttacgcata tctggtcttt agcagtagaa ggtcaaaact atttggtttg gccgatcttg
    37981 tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga tcttttatac tgtgttaggt
    38041 acatccttcc tctccgccat cttgatgatg atcttgtata caccaggagg cgatccaacc
    38101 cgtgtttatt acggtaccga cactcgtctg ttttctatat ggttgggaag tgcgcttgcg
    38161 tttgtttggc caagtacgcg tttaaagaaa aacatcccga aacaagcgaa aaatattctg
    38221 aatatcgctg gaggaatctc gcttttggca ctgattcttt gcttcttctt tttagatgat
    38281 cattacaatt tcatctatta cggaggaatg ctcctagtga gtctgttatg cgtagtgcta
    38341 gttgcagtga cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa tccagtattt
    38401 acttggattg gcaaacgaag ctatggcatt tatctgtatc agtttccagt tatgattttt
    38461 tatgaagcaa aggtgactga tattgccgat catgttttgt tgcatacttt agtggaaatc
    38521 acattgattc taatcatcag tgaactttct tatcgatatg tggagcgtcc gctagcacgt
    38581 tttgattatg gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc gatcatcagt
    38641 aagagcaaac catgggttgt tccaggcaca ttacttgtag caattgcact cgttggtttc
    38701 gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc aaaaatcgct
    38761 gaaagtaaaa aattagcaga agaaaccaga aacaattcag aaaacaccac tagttcggtt
    38821 gaccaagcga tccttgataa gtacgagttg tccaaggcac aagggaataa ggcacaaaaa
    38881 ttagaactaa cggcatttgg tgattcagtt atgttggatg ctgcaacgga tctacaagaa
    38941 ctttatccaa aagtagtggt agatggagat gtcggacgac aattatatgc cagtgtaccg
    39001 tacattgaaa aattaaagga acaagatttg ttgaaagaca ctgttctgat tggactagga
    39061 acgaatggct cattctcgga agctcagttt gatagtgtga tggatgcaat cggtgatcga
    39121 aaagtatact gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg
    39181 atgcttgaac agatgacgaa aaaatacgac aatatgacgc tgattgattg gtacagctac
    39241 agtaatgacc atagtgactg gttttatgat gatcaagtcc atccaaatcc tgaagggatg
    39301 acacattaca ttcacttagt cagcgaagcc ttgctaggag gaagttcaag tacgaacagc
    39361 agtgagacaa acgtgacaga atgatagcta caaaaaactt caggcaaaaa catttgtttt
    39421 tgcctgaagt ttttttatat ttaccgtgtt tttaacattc atatagattc aggaagcaaa
    39481 acggttgagt gtgtctgtaa ataacgttct ttttgttttc gggtgagttt tttgaatgca
    39541 gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat catcttaaca
    39601 ggtcttcttt tttgaagacg agtatattta gcaccagtac cattgttatg ttcctctaat
    39661 cgtctagaaa gatcggttgt atatccacca taaaatgtct gatctttaca gaataatacg
    39721 taaaaataat gttcatttgc catagagcat ctcacgaatc tctgcagtgt attctccatc
    39781 catattgtaa gtgatcaatg gtggagcgac tttgaatccg tctaatttgc cttctttgat
    39841 tccttcaact aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa attggatcct
    39901 tttaggtgca attcgatagt cttccatgac ctgaatgatt tctaagaacc gatcaggacg
    39961 atggaccata gtaaagcgtc catttgtttt tagtagctta cttgaaatat cgatcacttc
    40021 ttctaacgtc gtatgaatct catgacgggc gattgccagg tgtttattag ggttcgtttt
    40081 atttgttggc agccctttga aataaggtgg attacagacg actaagtcac aagaattatg
    40141 tcgaacgaca gataagctgt ctttcagatc gatggtgtgg acagtcattt gttcatttaa
    40201 atggttaagc gcgatactac gtcttgccat gtctgctaga cgaggttgaa gttcgatagc
    40261 gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca actgcgccat ttcctgcaca
    40321 aaggtcaaca atcttccctt ttttaggtac acgggaaaag tgagcaagca gcacagcatc
    40381 taaagaaaaa gaaaacactt tggaactttg gataatcttg atatcttctg cgtataattg
    40441 gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct
    40501 gtattatact atttttatta aaaagaacca aggtggcttg tcaaacctct attttttatg
    40561 catactatga ccataagaag ggaaaggaag agactatgtt ttatcgattt atgcgcgggg
    40621 tagttcgagt actattagcc ttagttaatg gaaatgcccg ctatgagaat aaagaagttt
    40681 taccgcaaga tgagaattac atactagttg ctccccatcg tacttggtgg gacccattat
    40741 atctggcggt agctgcagca cccaaggaat tcagctttat ggcaaaagaa gaactgttca
    40801 aaaatccagt tctgcgattt atattaactc gatccaatgc ttttccagtt aaaagagaaa
    40861 atccaggacc aagcgtcatt aaaacacctg taaagatttt gaaatcgaca aatttgagtt
    40921 tgatcatgtt tcccagtggg acaagacatg ctacagaact taaaggcgga atggcattga
    40981 tttccagaat gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg
    41041 gggatctgtt caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc gacttatcag
    41101 atatcaagaa aacggataaa gaaggtatgg cggagatcga acgacgaaca caagcagctt
    41161 ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa
    41221 atgataagtt tatgcgtggg cggtcttttg aatctactgt cctgttcagt tcagactttt
    41281 tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa
    41341 tagaagacag gtgtcatgta atggatcaaa gtgttataat aattagaagg acattttttg
    41401 aggtgattga atgcgttttt tacatacagc cgattggcat atcggaaaaa aactacaagg
    41461 ttatgacttg attgaagacc agcgtcatgt actatcttct atactagata tcgcgaaaga
    41521 agaaaaagta gaggcaatcg tgattgccgg agacttatat gataggagtg tcccagctgt
    41581 tgatgccgta gaattataca atgaattgat ggtcgattgg aatttaaagg aaaagatccc
    41641 gatttttgct atatcaggaa atcacgatag cagtacgaga ctttcagcgg gtacgccatg
    41701 gttcagttat tcgaactatt ttttgcgaac acagctagca gaagcatttg aaccagtcga
    41761 gtttaaaaac gtacaatttt tctttcttcc ttactttgaa ccaattgcag cgagattata
    41821 ttttgaagat gacaatatcc gaacgatcaa gcaagcggta gccaaggtga tagataaaat
    41881 gcaggagcta tttgattcag aaaaaaaaca agtattgatt agccattttt ttgtaatggg
    41941 aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc gggttggatt cgatccctgg
    42001 tgaactactt acaccgtttg attatgtagc actaggacat ttgcataatc aagctgcttt
    42061 gagacaggat aatgctcgct atagcggttc cccattgaaa ttctctttat ctgagatcaa
    42121 ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc ctggagttga attttaggga
    42181 gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc
    42241 tgccttttat cagtccgtca atcgggagaa ttacatctat atctatctca ctgatagaac
    42301 gatcatcccg aatatgatga accagcttcg gcaaatatat cctcgtattt tggggattga
    42361 acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga
    42421 tcctcaaaaa ttagcagaag cattttttag tgaaatgacg gaagagcagc taactgcaaa
    42481 ccaagaaaaa tggctcggag aaacgttgca agaaatcatg gaaatggaat gatcaagaat
    42541 gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt atcaatgaga ccgtagattt
    42601 ttcacgtttg acagaagcac cgcttttttt gatcagcggg aagacaggag ctggcaaaac
    42661 gacgattttt gatgggatca ccttcgcact ttttggtgaa acttccggaa gacttcgctc
    42721 tggaaaagag atgcgttctt tgtttgctac accagacgaa gaaacaagcg tgactttttc
    42781 ttttgaacat cagcagatga gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa
    42841 acgaaaagga aacggaacaa aaaaacaggc agccaaagta acgttgacca tttttgatgg
    42901 gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa gcagatcagt tgatcagaga
    42961 actgatgaat ctcgatgcga aacagttttc tcaaatcgtt ttactacccc aaggagaatt
    43021 ccgtaatttt ttggtttcat caagcagtga taaagagact gttttacgtc atttattcgg
    43081 tactcaattt ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca
    43141 aaaattggat catttagagc aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa
    43201 agaagaggaa ccagcagcag ggtttgaagc cgttttagcc caatggacaa accatcaggc
    43261 aatactcgaa aaacaaatca cggaagaaaa agaagctcta acggaacttc aggaaaaaca
    43321 aaagcaacta gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa
    43381 aacgatatta ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa
    43441 gaaacgatgg attgggtatt atgaatttac gcaacgatta gtcgaaccaa tccagcgcct
    43501 acaagaaata aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa
    43561 actagcgatg actacgcaga attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg
    43621 ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa aacaaacaga tgatggtgcc
    43681 aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa caattaacaa tttcattgga
    43741 taaagaagaa ttgaatgccg agaagctgta tcaaaatctc gctatctatc aggaaagaac
    43801 tcgaattctt caagaaaatt tactggatca ggaaaaactg caagaagatc gtcttgcttt
    43861 tggacagtta aaatatctag atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga
    43921 cactgggtcg ctcgcacaga taagagaaga actcaaaaaa gcaactgaag aaataacaaa
    43981 aaatagtcag ctattgaaag aacagacaga acaagcagaa cagctgaaaa gccaatgggc
    44041 aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca ggggagccat gtcttgtttg
    44101 tggatcaacg gaacacccca tacaacaatt aaaacacgaa cagccaaata aggaagatat
    44161 cttaaaagta gaacaacaac tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat
    44221 cattgccaaa agtgaagcac atgaaaataa tcttcaacaa caattatcag atttagaaca
    44281 aaaaatcatg gcaacgaata tacaattggc tgaacaagaa gaacacttgc aacaggcttt
    44341 gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa acaactcttg tagtaatcga
    44401 acaatcttta gatagacgaa aaagcaagat ggccaatgca agaatagagc tggaagaaat
    44461 aagacaacaa gcagaaaagc tcgaacaaca attagctgtc tacaaacagc agcagacaga
    44521 aaaaaataat cagctccagc aactgcaagg agaaatcaaa agtttagaac aacaattgca
    44581 agatacggat agtaactgtt tagaagagga gatacgacga cttgaaaaac agttagaaga
    44641 agactcgaat caactagctg ttgagcagaa agcaggagtg caattgttgc aagatcttca
    44701 tacgcttcgg acacagcttg atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat
    44761 agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat
    44821 tgaagaaatc tgtgcatttg cacaacagca agcacaatat aaagaaaacc aacgtacagt
    44881 tacttcttat catgaagagc aattagtgac agctgaccgg ctgcgtcaat tagaaaatat
    44941 ccagctaact gagacattcc cagatacaga agagatcagc caacagttag aagaaataag
    45001 acgtatgatc gaaataaagc aagaaaagat atacggttta caagaacaaa aaattagtaa
    45061 tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca agccagacga aattagatga
    45121 gcttacgcaa ttacagcagc tttcgcaaac attcaacgga gaaaatccta agaagactag
    45181 tttggaacgg tatgtgcttc aggtttatct tcaagaagtg ttacaagtag caaatgatca
    45241 tctccaacgt ttaacgaaaa atcgttatca atttgagcta gcggatacca ttggcagtta
    45301 tcgagggaaa accggattag aaatcaatat ctatgatgat caggcaggta tgtctcgaag
    45361 tgcgcatacc ctgtctggcg gtgagagttt tattgcagca ctttctttag cattagctct
    45421 tgccgatgtg atccagacac aagctggtgg tgtagcaatc gatgcattgt ttattgatga
    45481 aggatttggg tcattagatg aagaagcttt ggaaatggca atggaagctt tagaaacgat
    45541 cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta agagaattga aagaacgagt
    45601 attacaacaa ataagaattg atacagaagg tagcggacaa agcaaaatac gctatcaact
    45661 tggataaaaa gggagattgg taactatgaa atggagtatt ccagaaaaag tcattgcacg
    45721 tggacgtcaa tacttgcaag aaggccgagt gttatcggtt gtccctgacc aagaagaaaa
    45781 cgtgtggcat gcagaagtct tgggcagtga attgtatatg gttgatttgg atggtacagc
    45841 aaaagagata gattactgcc aatgtcccta ttgggaggaa cataaatatt gtaagcatac
    45901 agttgctgtg gaactttatt tacggtcgaa aaatctatct cgtatcatga aaaaagacca
    45961 gccaatgcta gaaccggtaa aagcaacaag tgagggagaa atcattgcaa aaggtttttc
    46021 tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct ttgattattg aataccaagt
    46081 agaaacgatt gagactaatc agtatcaccc agagctttct attttggcac tttacttgcg
    46141 tatcggttca ttggcatcac cgaaaaaaac atatatcgta aaaaacatct atgatttttt
    46201 acaagcttac acagaaaaag aaacttacac tgttaataaa cagtaccagt ttcgtctaga
    46261 taaacgggcg tttcaaaaag aagatcaaaa aatactcacg catcttgcag gaagtgctca
    46321 gacgcatcaa ttattaggta cgaacggctt acaagtcaaa ggaaaactag ataaaagata
    46381 tctgcttctc ccagtagaac aagcccgctt cctgttagag cagatgaatc agactacacg
    46441 tttgcatctg cagataaatg aacaaaacga tcaaggaata caattttccg atcaaaacaa
    46501 acctttatca ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag cattgaatga
    46561 ttttgatttt ttctttatgc attatcagtg gggagttatc caagggacat tttattctct
    46621 gacgaaatat caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc gattggagaa
    46681 accagaagta gtgttcaaga aaaaagaatt accgtctttg tttaaagaga ttatccctct
    46741 gctgtcagag atcggcgacg tagctattgc ccctgcagta gaagaggaag tagcagatat
    46801 ccccgtcgaa tttattttct ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga
    46861 tttcgtctat ggtgaagtca tctattcgac tgacccgaag catgaggtct cgacagaaca
    46921 ggcttttcag gtgttacgtg acacagtaga agagaaacga gtacttgatc tattcagaat
    46981 gtatcgctac cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg gggaagaact
    47041 atatgccttt ttccgcacag aactatctgt cttccgtcag tttggtgaag tcagacttgg
    47101 aagaaaattg cgagagttgt atttagatgc gcataaacat caaccgaaaa tcgaagtaac
    47161 cgagaccggt tcatggctcg atattcgatt cgatgtgaca gggatagagg agcaagaaat
    47221 cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt tatacattag aaaatggtca
    47281 ggttctttct tttgattcag aagaatttca acaaacaagc caagtgttgc aacaattacg
    47341 ggaatcgata cgtacagaag aaggaacgat ccatgtacct aaaaatcaag gattgatcat
    47401 tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca
    47461 agatctcatt catccagaaa gataccaggc acagcttccc aaaggattga atgcaacgat
    47521 gagagattat caaaagcaag gttttcgatg gttgaagatg ttaggtcatt atcagtttgg
    47581 cggtatttta gccgatgaaa tgggacttgg gaaaacacta cagacaattg cctttttact
    47641 ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc gtagcgcctg cgagcttaat
    47701 ttacaactgg caggcagagg tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa
    47761 tgggaataaa aaagaacgtg aagaattact ggctaaagac acagatatcc gagtgacttc
    47821 ttatgccagc ttaagacagg atctagctaa ttatcagtcg caaaaaattg attatttgat
    47881 tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag cattgagaga
    47941 attagctgta cctcagcggt ttgcactgag tgggacacca atcgagaata atttagaaga
    48001 attgtggtca ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa caaaattcag
    48061 ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg tttattttac ggcgagataa
    48121 acaaactgtg ttgaaagact tgccggaaaa aacagagatg aatctatatt ctgcacttac
    48181 agaagaacaa aaaactgttt atttagctta cttgcgtcaa atgcgtgaag aaatcacatc
    48241 aatggattct gaagcattca agaaaaaccg gatcgggata cttgctggtc tgacaagact
    48301 gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg gttccggcaa
    48361 gctcgaacaa gtaaaagatc tactggtagc tgccaaagag aacaaacgaa gagttttgct
    48421 gttttctcaa tttacaggga tgctgactat cttgcaagaa gagttagccg agctagggat
    48481 ctcgacattt tatttaagag gcagtacgaa accacaagat cggttaagta tggtagacgc
    48541 atttaatgct ggcgaaaaag atgtttttct gatttcctta aaggctgggg gaactggatt
    48601 gaatctcacg ggagcagata cggtcatctt gtatgacctt tggtggaatc ctgccattga
    48661 agaacaagct gctggacgtg cacatcggat cggacaaaaa aatgtggtag aagtgtggcg
    48721 tatgatctct gaaggaacga ttgaggaacg catggattca ctccagcaag aaaaacgcga
    48781 actgttccaa aaagtgatcc aaggtaatga agaacagttg acgaaactga ctgaagaaga
    48841 catccgcatg attttaagtg taggagaaat cgaggagtaa gagactcgaa aaagcgttca
    48901 gtaatgggta agaaagcctt tctaaagaag cgattgttta tttgatttaa aaaatagcaa
    48961 ggaggctggg attccatttt atcccagctt ccttgctatc atttttttat ttctattttt
    49021 aaaggttata caaagattgt cttcattgtc tctaacagct gatcaaaaga agaaatctcg
    49081 atatcgcaaa ttggagtctg ttcaattttg cgttctcgat ggttgaacca aagcgcttgc
    49141 cagttagcac tttttgcacc tagaacatca ttgtcgtaat tatcgccaac gtataaggtc
    49201 tcttctggaa gcatatgaaa ttctttctca gccagctgaa aaatctcttt ttctggtttt
    49261 tggaaacctg ttgcttgaga aatgagcata ttgcgggaag gaatccaatt gttcaattga
    49321 agttgcttca gtttttttgt ctgatggtca gtcggcccat ttgtgataat tcctaatggg
    49381 acattctttt ccttcaagaa atcgagggtt tttttgacct cctcatgcaa gcagatatta
    49441 tcaagttctt cctcgtaaat tttttgaaat aagaggccat cttcttccgt aatatgaggg
    49501 tagtctaaat cttttagtga ttggctgatc cgatgtgctc gcatatattc tagtgtccaa
    49561 tctcctgcca ttacttttgg gaagttttca tcactatgat ggcggaaccg gatatatagt
    49621 ggatgcatat ctgcatctga aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc
    49681 tgctgctgat catacatcgt atcatcaaca tcaaacacaa ctgatttaat caaaatttta
    49741 cacgcctttc ggttttcatt gattttttca caaatccttg actattgtaa cagaaaaaat
    49801 tgtctaaaaa agctgataaa taaaggtttt tcttactata tttctatttc cttgacagaa
    49861 taaggttttt atatttcagt ttttttcact aaaaatcgtg aaacacgtca ataagtttga
    49921 tgaacaaatt aatcattatg gtagaagtcg attgatttat tttaatgcaa tctctgtgga
    49981 attttcgaaa ga
//
LOCUS       DAZKNM010000004        47998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000004 DAZKNM010000000
VERSION     DAZKNM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.004"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..597
                     /locus_tag="ACG8M9_000155"
     CDS             226..597
                     /locus_tag="ACG8M9_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0376961.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(871..1563)
                     /locus_tag="ACG8M9_000156"
     CDS             complement(871..1563)
                     /locus_tag="ACG8M9_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0376962.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1566..2351)
                     /locus_tag="ACG8M9_000157"
     CDS             complement(1566..2351)
                     /locus_tag="ACG8M9_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0376963.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2588..3988
                     /gene="lacG"
                     /locus_tag="ACG8M9_000158"
     CDS             2588..3988
                     /gene="lacG"
                     /locus_tag="ACG8M9_000158"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0376964.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4013..4330
                     /locus_tag="ACG8M9_000159"
     CDS             4013..4330
                     /locus_tag="ACG8M9_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0376965.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4343..6016
                     /locus_tag="ACG8M9_000160"
     CDS             4343..6016
                     /locus_tag="ACG8M9_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0376966.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6030..7661
                     /locus_tag="ACG8M9_000161"
     CDS             6030..7661
                     /locus_tag="ACG8M9_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0376967.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8152..9717)
                     /gene="guaA"
                     /locus_tag="ACG8M9_000162"
     CDS             complement(8152..9717)
                     /gene="guaA"
                     /locus_tag="ACG8M9_000162"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0376968.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9776..10699)
                     /gene="coaA"
                     /locus_tag="ACG8M9_000163"
     CDS             complement(9776..10699)
                     /gene="coaA"
                     /locus_tag="ACG8M9_000163"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0376969.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10895..11500
                     /locus_tag="ACG8M9_000164"
     CDS             10895..11500
                     /locus_tag="ACG8M9_000164"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0376970.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11808..12824
                     /locus_tag="ACG8M9_000165"
     CDS             11808..12824
                     /locus_tag="ACG8M9_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0376971.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12965..14266
                     /locus_tag="ACG8M9_000166"
     CDS             12965..14266
                     /locus_tag="ACG8M9_000166"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0376972.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14312..14974
                     /gene="deoC"
                     /locus_tag="ACG8M9_000167"
     CDS             14312..14974
                     /gene="deoC"
                     /locus_tag="ACG8M9_000167"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0376973.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14995..15393
                     /locus_tag="ACG8M9_000168"
     CDS             14995..15393
                     /locus_tag="ACG8M9_000168"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0376974.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15459..16541
                     /locus_tag="ACG8M9_000169"
     CDS             15459..16541
                     /locus_tag="ACG8M9_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0376975.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16753..18315
                     /locus_tag="ACG8M9_000170"
     CDS             16753..18315
                     /locus_tag="ACG8M9_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0376976.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18308..19441
                     /locus_tag="ACG8M9_000171"
     CDS             18308..19441
                     /locus_tag="ACG8M9_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0376977.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19441..20397
                     /locus_tag="ACG8M9_000172"
     CDS             19441..20397
                     /locus_tag="ACG8M9_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0376978.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20496..21662
                     /gene="deoB"
                     /locus_tag="ACG8M9_000173"
     CDS             20496..21662
                     /gene="deoB"
                     /locus_tag="ACG8M9_000173"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0376979.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21686..22504
                     /locus_tag="ACG8M9_000174"
     CDS             21686..22504
                     /locus_tag="ACG8M9_000174"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0376980.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22521..23228
                     /gene="deoD"
                     /locus_tag="ACG8M9_000175"
     CDS             22521..23228
                     /gene="deoD"
                     /locus_tag="ACG8M9_000175"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0376981.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23526..24212)
                     /gene="gpmA"
                     /locus_tag="ACG8M9_000176"
     CDS             complement(23526..24212)
                     /gene="gpmA"
                     /locus_tag="ACG8M9_000176"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0376982.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24384..24965)
                     /locus_tag="ACG8M9_000177"
     CDS             complement(24384..24965)
                     /locus_tag="ACG8M9_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0376983.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25098..25781)
                     /gene="rpiA"
                     /locus_tag="ACG8M9_000178"
     CDS             complement(25098..25781)
                     /gene="rpiA"
                     /locus_tag="ACG8M9_000178"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0376984.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26100..26513
                     /gene="rpsL"
                     /locus_tag="ACG8M9_000179"
     CDS             26100..26513
                     /gene="rpsL"
                     /locus_tag="ACG8M9_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0376985.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26597..27067
                     /gene="rpsG"
                     /locus_tag="ACG8M9_000180"
     CDS             26597..27067
                     /gene="rpsG"
                     /locus_tag="ACG8M9_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0376986.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27161..29248
                     /gene="fusA"
                     /locus_tag="ACG8M9_000181"
     CDS             27161..29248
                     /gene="fusA"
                     /locus_tag="ACG8M9_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0376987.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29420..30607
                     /gene="tuf"
                     /locus_tag="ACG8M9_000182"
     CDS             29420..30607
                     /gene="tuf"
                     /locus_tag="ACG8M9_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0376988.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31014..31322
                     /gene="rpsJ"
                     /locus_tag="ACG8M9_000183"
     CDS             31014..31322
                     /gene="rpsJ"
                     /locus_tag="ACG8M9_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0376989.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31353..31982
                     /gene="rplC"
                     /locus_tag="ACG8M9_000184"
     CDS             31353..31982
                     /gene="rplC"
                     /locus_tag="ACG8M9_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0376990.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32010..32633
                     /gene="rplD"
                     /locus_tag="ACG8M9_000185"
     CDS             32010..32633
                     /gene="rplD"
                     /locus_tag="ACG8M9_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0376991.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32633..32923
                     /gene="rplW"
                     /locus_tag="ACG8M9_000186"
     CDS             32633..32923
                     /gene="rplW"
                     /locus_tag="ACG8M9_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0376992.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32962..33795
                     /gene="rplB"
                     /locus_tag="ACG8M9_000187"
     CDS             32962..33795
                     /gene="rplB"
                     /locus_tag="ACG8M9_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0376993.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33838..34116
                     /gene="rpsS"
                     /locus_tag="ACG8M9_000188"
     CDS             33838..34116
                     /gene="rpsS"
                     /locus_tag="ACG8M9_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0376994.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34139..34486
                     /gene="rplV"
                     /locus_tag="ACG8M9_000189"
     CDS             34139..34486
                     /gene="rplV"
                     /locus_tag="ACG8M9_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0376995.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34500..35156
                     /gene="rpsC"
                     /locus_tag="ACG8M9_000190"
     CDS             34500..35156
                     /gene="rpsC"
                     /locus_tag="ACG8M9_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0376996.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35159..35593
                     /gene="rplP"
                     /locus_tag="ACG8M9_000191"
     CDS             35159..35593
                     /gene="rplP"
                     /locus_tag="ACG8M9_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0376997.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35583..35771
                     /gene="rpmC"
                     /locus_tag="ACG8M9_000192"
     CDS             35583..35771
                     /gene="rpmC"
                     /locus_tag="ACG8M9_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0376998.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35796..36062
                     /gene="rpsQ"
                     /locus_tag="ACG8M9_000193"
     CDS             35796..36062
                     /gene="rpsQ"
                     /locus_tag="ACG8M9_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0376999.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36120..36488
                     /gene="rplN"
                     /locus_tag="ACG8M9_000194"
     CDS             36120..36488
                     /gene="rplN"
                     /locus_tag="ACG8M9_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0377000.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36525..36833
                     /gene="rplX"
                     /locus_tag="ACG8M9_000195"
     CDS             36525..36833
                     /gene="rplX"
                     /locus_tag="ACG8M9_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0377001.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36861..37400
                     /gene="rplE"
                     /locus_tag="ACG8M9_000196"
     CDS             36861..37400
                     /gene="rplE"
                     /locus_tag="ACG8M9_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0377002.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37419..37604
                     /locus_tag="ACG8M9_000197"
     CDS             37419..37604
                     /locus_tag="ACG8M9_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0377003.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37640..38038
                     /gene="rpsH"
                     /locus_tag="ACG8M9_000198"
     CDS             37640..38038
                     /gene="rpsH"
                     /locus_tag="ACG8M9_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0377004.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38070..38606
                     /gene="rplF"
                     /locus_tag="ACG8M9_000199"
     CDS             38070..38606
                     /gene="rplF"
                     /locus_tag="ACG8M9_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0377005.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38767..39123
                     /gene="rplR"
                     /locus_tag="ACG8M9_000200"
     CDS             38767..39123
                     /gene="rplR"
                     /locus_tag="ACG8M9_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0377006.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39144..39644
                     /gene="rpsE"
                     /locus_tag="ACG8M9_000201"
     CDS             39144..39644
                     /gene="rpsE"
                     /locus_tag="ACG8M9_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0377007.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39659..39838
                     /gene="rpmD"
                     /locus_tag="ACG8M9_000202"
     CDS             39659..39838
                     /gene="rpmD"
                     /locus_tag="ACG8M9_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0377008.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39882..40322
                     /gene="rplO"
                     /locus_tag="ACG8M9_000203"
     CDS             39882..40322
                     /gene="rplO"
                     /locus_tag="ACG8M9_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0377009.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40322..41617
                     /gene="secY"
                     /locus_tag="ACG8M9_000204"
     CDS             40322..41617
                     /gene="secY"
                     /locus_tag="ACG8M9_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0377010.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41679..42326
                     /locus_tag="ACG8M9_000205"
     CDS             41679..42326
                     /locus_tag="ACG8M9_000205"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0377011.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42517..42735
                     /gene="infA"
                     /locus_tag="ACG8M9_000206"
     CDS             42517..42735
                     /gene="infA"
                     /locus_tag="ACG8M9_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0377012.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42768..42884
                     /gene="rpmJ"
                     /locus_tag="ACG8M9_000207"
     CDS             42768..42884
                     /gene="rpmJ"
                     /locus_tag="ACG8M9_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0377013.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42902..43267
                     /gene="rpsM"
                     /locus_tag="ACG8M9_000208"
     CDS             42902..43267
                     /gene="rpsM"
                     /locus_tag="ACG8M9_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0377014.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43295..43684
                     /gene="rpsK"
                     /locus_tag="ACG8M9_000209"
     CDS             43295..43684
                     /gene="rpsK"
                     /locus_tag="ACG8M9_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0377015.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43764..44702
                     /locus_tag="ACG8M9_000210"
     CDS             43764..44702
                     /locus_tag="ACG8M9_000210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0377016.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44730..45110
                     /gene="rplQ"
                     /locus_tag="ACG8M9_000211"
     CDS             44730..45110
                     /gene="rplQ"
                     /locus_tag="ACG8M9_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0377017.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45382..46221
                     /locus_tag="ACG8M9_000212"
     CDS             45382..46221
                     /locus_tag="ACG8M9_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0377018.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46197..47066
                     /locus_tag="ACG8M9_000213"
     CDS             46197..47066
                     /locus_tag="ACG8M9_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0377019.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47056..47853
                     /locus_tag="ACG8M9_000214"
     CDS             47056..47853
                     /locus_tag="ACG8M9_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0377020.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15178 a         8772 c        10519 g        13529 t
ORIGIN      
        1 tagagtttct aaagtttgtt tcttaattag cttattgatt ctataatatt tttgatttat
       61 ataggaaatt aaaaatatta tagaatcaac actcagagaa cgggttggtt tttaatagat
      121 ttaataagta ctgataatga aatttctcct ggttgggaaa acgaagttgg ttaccctgta
      181 gaagaatttt ttcaagatta ctttgcggag tatgccgata ggataatgag agattacgta
      241 acagataaaa ctcaagaagt catcaatcaa tcccagtcat taaatgaatt atctgataag
      301 gcgaaagaag aagcagttgc cattatttct tcagggatta gtgactatat tgatatgagt
      361 ggtgaaatca attatggcga taaagacaaa attgtacgca tgataaaagc ttttatcaaa
      421 acttttgatg tgaaggcaga tcttaagttt aatgatactt acctagtagg aaaattaatt
      481 aatatccttg ggacagagaa agatacggaa acgtttattt cctctttgtc tatgttgttt
      541 acagataagc atttttcaac tcagcttggt ggagaggaac tagtggaaat cttttaacca
      601 ttacgcccag cattaataaa agtatatcaa aaaagtactc ctgatgattt ttaagattgg
      661 tttgagaaat aatgtgaaaa cactattttt aatgttaatt atagatagag atgaacgcgt
      721 catatggtta aaagaatgaa aaagtcaaag atacactgct atatttcact aaggatatag
      781 cagtgtatct ttcatgtcgt ttcactggca gttttttata aaaggaggtg tgcatttcag
      841 ttgcttttct ttggtagtta gttgaatcgt ttaagaatta acggatgtgg cagacaggtg
      901 ctctgtccaa aaaccagtaa aatctttgat ggttccatct ttatttagct gtgcctgttc
      961 aaatactgtt ttgtttttac cgaatggatc aattgcaata attgttccga tccccgcacg
     1021 tttggcagca agtaatcctg agtaagcatc ttcgataaca agacaatcag ccggagctaa
     1081 gctcagtttg tctgccgctt taagaaaaat atccggttgc ggtttcccag ggaaagagcc
     1141 gtcgtcatag acaatcttgt cccagtcaaa ccagcgggct aaatcgaaaa tatcaaaata
     1201 aaaagcaaca ttctctttga cagtagccgt ggcaatcgtt aatggaatct gttggtcagt
     1261 cagctggtca agagtcgctt caagcccttt tgtcaactga agctcgtttt cattttgcaa
     1321 gcaaagcata cgataatagc tttctttttc ttctgacaat acggcgatct cagaggcggt
     1381 caaatgctct gaaatgaaat gtttcaaaat ctccgtattc gtccgaccat gaatattttt
     1441 taaaatatcc tcgtctgata aaccatcttt tgtatgatca tgtatcatat gcagccaagc
     1501 agcttcatgc agatgagagt ccaaaaacat tgttccgttg aaatcaaata taatcccttt
     1561 catagttacg ctcctatagt ggtattttcg gttgaatcag caatttgcag atcgacatat
     1621 tgtgcatagt gctcttgaac ctcccgcgag atctggcgat ccgtaatgag cagatcgaaa
     1681 tcatgcaaat tataaaacac ataaaaatca tctcgattga attttttatt atcaacgagt
     1741 agatatttct tacgggaatt atttagagcg atctgctgtg cttctccttc ttcggtactg
     1801 taagtagaga tcgcttcgtt gtgaacgcca tttgaactga taaatgcttt tgtaaacttt
     1861 agtcttctta aattgtcgtt ggtgatcggt ccgacaaatg cgccggtatt tttccggaaa
     1921 tcaccgccaa tcagtagaat ttctggtcca tctgcttgag caagaatatc aaaaactgga
     1981 taattatttg taatgatccg aattttcttg ttcagtaaat gcgtcgccaa tagttcgatt
     2041 gttgtacctg gtcctaagaa aatcgtttct ccctcgtgaa tcaaggaggc tgctgtggca
     2101 gcaatttctt ttttttcttg aatctgcaga gtggcttttt cagtgtgaga aagttcttga
     2161 tctaaagtgt aggaaagact ttgagcccct ccatggacac gcagcagctt gcccgatttt
     2221 tctaattcat ctaaatctct gcgaacggtc atatcagaaa catctaattg attgatgatt
     2281 tcattaacgg taaggattcc ttttttattg accatttcta cgatagtaag caatctttct
     2341 tttttcaaca ttgtcttcac ctctaattta aggataatct atcatataaa cggacaacag
     2401 tcaatctaat tcgaacaaaa gtaaacattt agggtggttt ttatgtttgt tacaatagat
     2461 tttattgctg ttttctggtt tttatgatta tttgtgtttg tttttgttga caaatgtttt
     2521 ggatgcgttt acaatgcaat cagttagaaa gagcgaaaac tgattataga tttgaggaga
     2581 gatgtacatg cttaaattac cagaagattt tatttttggc ggagccacag cagcctatca
     2641 ggttgaagga gccactaagg aaggtggcaa aggcgctgtt gcttgggatg actttttaga
     2701 ggaacaaggg cgattcagtc ccgatccggc aagtgatttt tatcatcaat atgcaaagga
     2761 cattgaattg tgtgaacgtt ttggtgtaaa tggtctaaga ttatccattg cttggagccg
     2821 catttttcct gatggagctg ggaaaccaaa tccagaagga atcgcttttt atcatcgtgt
     2881 gttcgaagaa tgtaagaaaa gaaacgtaac gccgtttgtg acgttgcatc attttgatac
     2941 ccctaaacgt ttgtttgacc atggagattt tttgaatcga gaaacaatag aagcatttgt
     3001 ttcctatgcc attttttgtt ttcatgaatt caaagaagtc aaggtgtgga gtacctttaa
     3061 tgaaatctat ccagttgcaa ccaatcaata tttgttaggt gttttcccac caggaatcaa
     3121 atatgatttt acaaagatcg tcgcctgtct tcataatatg atggttgctc atgcccgtgt
     3181 agtgaactat ttcaaagaaa acgaacttcc gggtgaaatt ggtgtagttc actcattaga
     3241 gacgaaatat gcggcaacag acgcgccgga agataagcat gctgcctttt tagacgatgc
     3301 attgtcgatc cgtttcttat tagatgctac ctatttaggc tattattcaa ctgaaacctt
     3361 gactgctctt gatgagatat gtgaagcaaa tcaggccagc tatcattttc ctgaagaaga
     3421 tttcgttgaa ttgaaaaaag catccacgcg taatgactac ttagggatca atcactacca
     3481 atgccatttt gtcaaagctt acgatggaga aaatgcaatc catcataacg gtacgggtga
     3541 aaaagggacc tctgtttata aagtgaaggg aatcggagaa cgtatttata aagaaggaat
     3601 tcctcgaact gattgggact ggctgattta tccagaaggt ctatatgact tgttgctgcg
     3661 aattaaaagc gattacccac attacaacaa aatctatatc actgagaacg ggatggggta
     3721 taaagatcag tttgaagatg gcattattat ggatcagccg agaatcgact atttgagagt
     3781 ctatttggaa tccttaagca aggcgataac agccggggta aatgtgaagg ggtatttttt
     3841 gtggtcgctt atggatcttt tctcatggac aaatgggtat aacaaaaggt atggcttgtt
     3901 ttatgttgat tttgaaaccc agaagcgtta cccaaaagaa tcagcttact ggtacaagtt
     3961 ggtcagtgaa accaaaacga ttatttaata agtgataatg gaggaagaaa agatgaataa
     4021 agaggaatta caaatgcttg gttttgagat cgttgcatat tcaggcgacg ctcgcagtac
     4081 attattgaaa ttattgaaag aagtaagaca aggaaacttt gaacatgttg attcggcgtt
     4141 gaaagaagcc gacgaaaatt taacccttgc acataattcg caaacaaaaa ttttagcgga
     4201 agaagcatct gggaaagaga tggagatggg attcattttt atccatgggc aggatcattt
     4261 aatgaccact ttattgctga gagatctgat tcaagacttt attgtactgt acagacagaa
     4321 tcaaggatag gagaagaacg ctatggatgg aatcattaaa caaatcgaaa aagggaaacc
     4381 attttttgaa aaaatctcac gtaatagtta tttaggagcc gttcgtgacg gatttttaac
     4441 agcgatgcca gctattttat tttcaagcat tttcattttg attgctgcgg ttcctgagat
     4501 ttttggttac caatggcctg aatctgtctc cacatggcta tggaaagtct ataattattc
     4561 gatgggtgtt gtagggttac tcgtttcagc aaccactgct cgctgcttgg ctgattcgat
     4621 gaatcgtcgc ttaccgaaaa acaaagtaat caacaccacc tctgtgatgc tggcctctgt
     4681 ggtaagtttc ctgttgttaa gcgtctctcc gattgaaggt ggattttcaa cggaatatat
     4741 ggggacaaag ggactactgg cttcctttgt cgctgcattt ttaacagtta atttatataa
     4801 gttttgtgtg ctgaaagata ttaccattaa aatgcctaaa gaagttcctg gaacgatttc
     4861 gcaaaccttc cgagatattt ttcctttctc tttttcagta tttgcggccg ttttaattga
     4921 tttaggcatt cgacaaatct tccaaatctc atttgcagaa gcaatcatta cggtgctgca
     4981 gccgctattt acagcagctg acgggtactt aggaatcgct attatctggg gagcgatggc
     5041 gctgttttgg tttgtcggtg ttcatggacc gtcgatcgtg gagccggcaa ttgcggcaat
     5101 tatctatgcc aatgtggaaa ccaatctgca attgttcaat tccggtgaac aagcttctaa
     5161 agtactgacg gtgggtttag ggaattttgt aggaactatg ggaggaactg gggcaacatt
     5221 ggttgttcct tttatcttcc tactctttgc taagtctaag cagttgaagg cagttgggaa
     5281 agcttccttt attccagtaa gtttcgctgt caacgaacca ttgctatttg cggcacccat
     5341 gattttgaac ccttattttt ttattccatt tttgttaaca cctgttgtaa acgtttgcct
     5401 gttcaagttt tttgtcgatg tattgggcat gaacagcttt atgtacgttt tgccttgggc
     5461 aactccagca ccgatcggtt tggttctagg aacgggcatg ggtatcttag cttttgttct
     5521 agcagcggtt ctaatcgtcg ttgacttcct gatctatcta ccattttgca aagcatacga
     5581 tgctgtctta gtagagaaag aagcacaaaa agcagaggtg atcgcaaccg aggaaggtgc
     5641 ttctgccgat gtacgtgagg aagtacctgt ggcagcgagt caagttatca gcgatgcaaa
     5701 taaagaaatc aatgtgttgg tactttgtgc tggcgcaggg acaagtggga tgttagctaa
     5761 tgctttaaaa aaaggtgcat ctgaatcgaa agcaccgatc agtgcaatgg cgggtgctta
     5821 tggttctcat tacgaaatgc tgcaggattt tgatatggtg gtcttagcac cgcaagtcaa
     5881 ttcttactat gatgatatca aagaagacac cgatcgctta ggaatcaagc tggttgcgac
     5941 aaaaggagca gaatatatcg gattaacgag agatcctgaa aaagctgttc agtttattct
     6001 gaaccaattt caataaagga ggagccataa tgaaaatggg aatttggttg cgtaaagctg
     6061 ttttatttgc agctgtgtta ggggctttgg gggctgtttc aaaaactgtt gaagcatctg
     6121 ccagcgaatt tattcgcgga gcagatattt cgatcctgtc agatatggaa aaatctggtg
     6181 caaaatatta tgaagacgat atttcaaaag atgccttaga gattttaaaa aataatggtg
     6241 tcaactatgt tcgactgcgt ttgtggcagg atccttataa tagtaatgga gaaagttatg
     6301 gagcagggac aaatgatctg gaaacgacga ttgaccttgc aaaacgagca aaaaatctgg
     6361 gacttaaggt gctgctggat tttcactaca gtgatttttg ggttgatcct gggaaacaga
     6421 atcttcctaa agcatggcaa ggtctgacct ttgaagaaat gaatacggca ttgtacgact
     6481 atacaaagaa cgttctgtcg gaaatgaagc agctggacgt ttatcctgat atggttcaga
     6541 tcggcaatga actgaatagc ggaatgctct ggccttatgg caagtcttgg ggagaaggcg
     6601 ggggagaatt tgatcgattg gctgcttttt tgaagtctgg tatccaagct gttcgagaca
     6661 ctcaacctaa aacaactcca gtaatgctcc acttagctga tggtggcgat acaggagcat
     6721 ttacttggtg gtttgatgaa atcacgagtc gaggtgtgtc atttgattta attggagtgt
     6781 cgtattatcc gtattggcac ggttcaatga atgaacttca aaccaatatg aatgcgatca
     6841 gccaacggta tggtaaagaa gtgatcgtag ttgaaacagc ttatgggcat acaacagcga
     6901 atgccgatac tatgccaaat gcttttggtg aagccgaagc agctgcgggg ggatatgaac
     6961 caacaccgac aggacaagca gagtatttgc tggatcttgc cgatcggatt caggcagtac
     7021 cgaataatcg cggggcgggc ttcttctatt gggaacctct ttggtacaac ggaaatgtga
     7081 gttgggcaac acaagcaggt atgaattatc tcagtgttca atccactatg ggaaacgaat
     7141 gggacaacca agcgatgttt gattttgctg gaaacgcctt gccaagcttg cgtgcgttta
     7201 aacaagcagg ggcgcaaata aatctagtta aaaatgcaag tttcgaaagc gatggaccaa
     7261 cgaccagtcc ttccaattgg caaacctggt ttcaacaagg aaccgctaag ggtacgatca
     7321 aaacggaagc gggcactttt tttggggact acaaactaac tttttggcag gatcaagcat
     7381 acgaggcatc agcttatcaa aatgttagtg gcataagcaa aggaacttac acattgagtg
     7441 cctatgtaat gtctggggga gagtttgata ctaatcagtt atatatcaaa aattatggtg
     7501 gagcggaact aaatactgcg gttgttaaaa caagtgaatg gacaaaaatt acaatcaaag
     7561 acattccaat tacaacaggt gtttgtgaaa ttggggttta tagcaaggct tcaggaaatg
     7621 cttggacgaa cgttgatttt gtttcattgg tgaaacaata gactgcggat cgtttcgatt
     7681 caagatcgga ggttcccttc agccacatcg ttagcgtaga aggcaggaat cgaacctgca
     7741 cctcgccttt gccaaaaata accagaaacg atttccagtc atttttacca aaacgagtaa
     7801 agcaccagac ttcatctaca ctttgtatta tgctaaagag agttcaacga tttcgtaaga
     7861 agcaatgaaa aaattgcaaa aagttgtgca gataacataa agtttgttta agaaagggta
     7921 accttagaat tccgtcgtga atatgatttg agcacttaga tggatagaaa aaagttctcg
     7981 gaaaacagtt cgtttttcga gaacttcttt ttatgataag aatgaaacaa attaacttat
     8041 attatagtct cttttggtag ggaaccatct tatttttaat gaaaatcaag cattttagct
     8101 attgaagaat gttgatttat agggattccc cctccatatt atgttggctc tttattccca
     8161 ctccactgtt gctggtggtt tagaagtgat gtcatacacg atacggttga cgtgggctac
     8221 ttcgtttacg atacgtacag agattttttg caagacttcc catgggatac gggcaaagtc
     8281 agcggtcatt ccgtcgattg aagttacagc acggataccg actgtgtagt cgtaagtacg
     8341 tccgtcaccc ataacaccga cagagcggat ccctggaaga acagtgaaat attgccagat
     8401 atcacggtca agtccagcag ctgcgatttc ttcacgcaag atagcatcag attcgcgtac
     8461 gatttctagc ttgtcttcag tgatttcgcc aagaacacga ataccaagac caggaccagg
     8521 gaatggctga cgccaaacga ttgaatcagg catgcctaac tgagtcccta attcacggac
     8581 ttcgtctttg aataatgtgt ttaatggttc gatcaattca aattgcatat cttctggcag
     8641 tccacctaca ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag attcgataac
     8701 gtcagtatac aacgttcctt gtgctaggaa agagacgcct tcttcaccag caagttttgt
     8761 tgcttcgtca tcaaaaacat atacgaattc attcccaatg attttacgtt tttgttctgg
     8821 gtcagataca cctttcaact tgtctaagaa gcgatctctt gcattgactt tgatgatgtt
     8881 caagccgaat tttccgccta gactttccat tacttgttcc gcttcgcctt tgcgtagcag
     8941 accgtggtca acaaagatag atgtcaattg gtcaccgatc gctttttgta aaagaacccc
     9001 aacgacacta gaatcaacgc cacctgaaag accaagcaag acttttttgt caccaacttg
     9061 ttcacggatt ttttctactt gcatatcgat aaagttatcc attgtccagt taccttcaca
     9121 gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg aatgacgtac
     9181 ttctgcatgg aattggatac cataaaaatt acgtgttgtg tcttggatag aagcaatcgg
     9241 acaatctttg cttgttccga ctgtttcaaa gccttcagga actttagtta ccaagtcacc
     9301 atggctcatc catactgttt gttgttttgg tgtttcttta aacaagacag cttgatcgct
     9361 tgttacttct aacaaggctt tcccatattc gcggttttta gcaggctcta cggaaccgcc
     9421 aagtttgtac gtgatcaatt gcataccata gcaaatccct aatacaggaa tacctaaatt
     9481 gaagatctca ggatcgatcc caaatgcatt ctcatcatat acactgtttg gtccgccaga
     9541 aagaatgatt ccctttggat tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa
     9601 taattcggaa aagactccaa gatcacgaat acgtcttgtg atcaattggt tatactgact
     9661 accgtagtct aatacgataa tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt
     9721 tattacccct atccctttaa attttatctc aaaatagtca aactatttta aaatgctaat
     9781 attttcgcat gaaaatcgaa tcgatgatat gattttcagt tttatggaga attatatcgg
     9841 ctcttcctcg tgtaggcaag atatattcct gtaaattttt caaattcaca tttttccata
     9901 catcttttgc cattttaaag gcttcttcac gatctccgat agcaaaacga taataataat
     9961 tctctggatt ttggaaagcc gtatccagta acgcaccaaa acgatccaaa taccattttt
    10021 cgatcagcac gggatcggca tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga
    10081 tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag atatcaggct
    10141 gctggatcaa ttcgtacttg tctggaatga tatcatacac atcatgtgag taaacaggta
    10201 cttttatctc ttcctgacca gattttacac ggttcaagaa atcgatcagc atctccatat
    10261 cataactttc aggaaatcct ttacggtcca tgattccacg ctcttttaat atacgatttg
    10321 gataaagaaa tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag gtacgagaca
    10381 aaatcgtctg cagcagtctg gctgttgtac tttttccaac ggctacactt ccggcgatcc
    10441 cgataataaa aggcggtact tctacgtatt tatgaagaaa taaaccttta ctcaagaaca
    10501 aagattcaaa ttctttcata taaagataaa tcagatgagc aagaggtaca tagatatctc
    10561 gaacatcctg catggaaatg cggtcattca aacttttgat attatctagt tcatcttgtg
    10621 tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc ttctcttgcc acacggtagt
    10681 agttcattgg atcattcata ttagcctcct gcgttctccc atcaatggaa ctattttaac
    10741 acaaaataaa cgcaaacccc accacttaag agagattgcc gatctttttg tataatcttt
    10801 ataatagaaa gaaacggagt cttattcttg acttgcgcac ggatacataa aaggatagac
    10861 tgttgtcatg aaagaaatga gaaggatgga agacatgaca aatcattact attctgaacg
    10921 cccagatacg gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca gaacctttca
    10981 atttgtaaca gattctggtg tattttcacg tgaaacagtg gattatggtt cacgtgtcct
    11041 gattgatgct ttcgattggg aagagttacc tgatgaagga aagatcttag atgtgggatg
    11101 cgggtatgga ccgattgggt tagctttggc atttgcgaca cagcggtttg ttgaaatggt
    11161 cgacattaat gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta accaaatcga
    11221 acatgttgat atccatcaat cgaatatcta tgaagcagtt catgaagaaa cctatgcagc
    11281 tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg
    11341 cgcatatcct cggttgaaga agggcggtac actgacaatt gtgatccaga agaaacaagg
    11401 agcgccgagt gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa
    11461 agataaagga tattatatca tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag
    11521 cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt atcagttctt tttttgttga
    11581 gaagttttta aaaacaaaga actatttttt cttcaaaaag aaatagtagg aacagagtcg
    11641 ttgtttttta cgataaaata aataagatac agaacttgta tagaaggctg ccaagtcggt
    11701 agataaagga ggtttttatc aaaaaacata ctttcctttt ttctctattc ttttcttggg
    11761 ggttttggta cactgatagg gaatttagat agaagggagc caataggatg aagctgacca
    11821 tcagagatat cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag attttgaata
    11881 acaaaggaag cagatttagt gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac
    11941 atcattataa gcccgattat ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg
    12001 gaatgatcgt ccctgatgtc actgatttct tcttctccaa actgatcgaa ggtgtagaga
    12061 gctatttgaa tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa
    12121 atgagatcaa gtatttacaa gagctttccc accgctcagt ggacggaatc ttgctggcca
    12181 caccaaatat tttacctgag aaatatgcat tagacagcgg tttttatcaa aaaatgccaa
    12241 tcattttaat cgatcgcgga ttgaatcgtc gggataacgg acggcttatc gtaaaggaat
    12301 atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa tggccatacg aagatcggca
    12361 tgctgaagga aaccactgga tactatcaat tagaagaacg tttcaacgga tatcgtcatg
    12421 cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt tgaacatgga gagttgactg
    12481 tccaaggtgg atatactgca tcaaaaaaat tactcaaaca taaagatatc acggctattt
    12541 tttgcgggaa tgatgccatg gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa
    12601 aaatcccaga agatatctca gtcataggat tcgatggatt aaagctgtca gaatacatga
    12661 ttccgcaatt gacgacagtg cagcagccaa gttttgatat tggattttat gctgcacgtt
    12721 tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa
    12781 aattaattat tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc
    12841 ttatgtaagc atttacaaag gctagttagt gcttttttta tttttagtaa accagtttac
    12901 taaagtggtt tacctttcct aaaaataatg tataataccg aatgaagaca aggaggatca
    12961 ggcaatgcga atggtggatc tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga
    13021 ggaaatcgag tacatcgtca cgaactatac gaatggaaag attccagatt atcaagtcag
    13081 tgcattattg atggcaattt tttatcaaga catgacaaat gaagaaatca cgaacttaac
    13141 attggcgatt gctaattctg gtgatgtcat cgatttgagt tctcttgaag gaataaaggt
    13201 agataagcat tcgacaggtg gcgttggtga cacgaccaca ttgattttag cccccctagt
    13261 agctagtgtc ggagtgacag tggctaaaat gtctggacgc ggtctaggtt acacaggtgg
    13321 tacactagac aagctagaag caattcccgg atttcagatc gagctttcgg atgaagaatt
    13381 tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa acttggcacc
    13441 ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg accgttgatt ctcttccgct
    13501 gattgccagt tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga
    13561 tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa
    13621 gaccatgacg agcattggga aactggctaa ccgagaaaca gtggcggtta tctcagatat
    13681 gtcagaacct cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac
    13741 attacaagga aatggtccag aagatctagt agaaatgtgc tacgccttag gaagccaaat
    13801 ggttgttctt gctggcaaag ctaagacaac cgatgaagca agaacgttgc ttcaagaagc
    13861 tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc aagggggaga
    13921 cccagcgatc gtggaacatc cagaacgaat tttgactgca cgctacacaa tggagcttcc
    13981 tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat
    14041 gatgctaggt gctgggcgta aaaccaaaga agaagatatt gatcacgctg ttggattgaa
    14101 actgcataaa aaaattggtg acactgtgac aaaaggtgag tcactattga cgatctatag
    14161 taacgacgaa gaaatcactt ctgtcattga attattatat aaaaatattg aaattggtga
    14221 atcggcaatg aagccaacgt tgattcatga catcatcaca gaataaagga aaaaagcgga
    14281 ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga tcgaccatac
    14341 tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa
    14401 ataccatttt tattctgtct gcatcaatcc agcatgggtt gcgctagcga aagaacaatt
    14461 acaaggtgag ccagtagccg tatgtacagt aatcgggttt cctctaggag caaacacacc
    14521 cgagacaaaa gcatttgaaa caacagatgc catcaataac ggtgcagatg aagtcgatat
    14581 ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga
    14641 agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt
    14701 attagataga gaagaaatca tccgagcttg cgaaatagca aaagctgcag gcgctgattt
    14761 tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag gtagaggatg tccgtttgat
    14821 gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga
    14881 agaagcaatg gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg caggagtagc
    14941 aatcgtatct ggttcaactg gtgaaggcta ttaatttttt caaaggagaa gctcatggca
    15001 gaactcaaac aagaatggat caacacagct attgaagcgt tagataaagc ctatgttcct
    15061 tattcccatt ttccggtagg cgcttgtctc gtaacggaat caggcaagat ttatcaaggc
    15121 atcaacattg agaatgcatc atttggatta acgaattgtg ccgagcgcac agcttttttc
    15181 aaagcagtat ctgaaggaga aaaaacattt acgcatctag tcgtagcggg gcacaccccc
    15241 gatcctatct ctccttgtgg tgcatgtcga caagtaatgg cagaattctg ttcccctgat
    15301 atgccggtta cgctagtagg agacaatggt gtgacgaagg caacgaccgt tcgtgcactg
    15361 ctgccttatg catttactga aaaagatcta taactcatgt tcattcagtc agctgactgt
    15421 tgaaaaataa aacaattatt ttaggaggct ttacagaaat gaaaaaagca aaattatttg
    15481 gtttaggtgc tgtcgcacta gcagcagggt tatttttagg tgcatgcggc aacaacggct
    15541 ctactgattc atccggtggc aaaagctcat cagacacaac aactgcagca ttgatcacag
    15601 acacaggtgg tgtagacgac cgttcattca accagtcagc ttgggaagga ctagaaaaat
    15661 gggggaaaga tcaaggactt tcacgaggaa atgatggctt ccaatatttc caatcttcaa
    15721 atgaatcaga ttacatccca aatattgacc aagcattgaa tgctggattc aaaacgattt
    15781 ttggtattgg ttataaacta aaaccagcga tccaagaaca agcaacaaac aatacaggta
    15841 cgaactttgt tattatcgat gatgtcatcg atggattaga taacgttgta tctgcaactt
    15901 tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc agcttatacg actgaaacaa
    15961 acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat
    16021 tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc
    16081 aatatgctgg tgacttctct gctccagata aaggacgttc aattgctcaa ggtatgtatg
    16141 cacaaaacgc agatatcatc ttccatgctt caggcggtac aggtaacggt gtattccaag
    16201 aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg
    16261 accaatcaga cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc acattgacat
    16321 ctacactgaa agcagttgga acagtagtag aagatttggc acaaaaatca gcggacggta
    16381 aattccctgg cggcgaacac actgtttatg gattgaaaga agacggtgtt ggtctaacag
    16441 aaggacaact ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt
    16501 caggtgacgt aaaagttcca gaaactcctg aagaaaacta aaaccatttc tatagccttg
    16561 ttagacacat gtttgacaag gctatggttt gattagaaca agaattgttc atttatccaa
    16621 gctgccttgt gacagtttgg ataagggaac aactccctga agtaatgttt gtttcaaaag
    16681 aagaacgctc actaagcgtg aatcttagtc aatcgttgcc ctagatagaa atcgttagga
    16741 tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa tgcagaatat taccaaagaa
    16801 tttggaacat tcaaggcaaa tgacaacatc aatttacaag taaaggcagg agaaattcat
    16861 gcgttgcttg gagaaaacgg tgctggcaaa tctacattga tgaacgtgct ttccggatta
    16921 ttagagccga catcagggaa aattttgatg cgtgggaaag aagtaaagat cacaagtccg
    16981 accaaagcca accaattagg gattgggatg gtccatcagc actttatgct tgttgatgcc
    17041 tttactgtaa cagaaaacat cgtgttggga agcgagccta gtcgcgcagg gatgcttgac
    17101 cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac agtatggatt gtcagtcaac
    17161 ccagatgctt acgttcgtga tatttcagtt ggaatggaac aacgggtaga aattttaaaa
    17221 acactttacc gaggagcaga tgtactgatt tttgatgagc cgacagctgt attgacccct
    17281 caagaaattg atgaattaat cgtgatcatg aaggaattag tcaaagaagg caagtcgatc
    17341 attttgatta cgcataagtt agatgaaatc aaagcagtag ctgaccgctg tacagttatc
    17401 cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg taacctcaca acaattagcc
    17461 gatatgatgg ttggaagaac ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa
    17521 gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg
    17581 aaaaacttga acttagaggt tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga
    17641 aacgggcagt cggagttgat ccaagctttg actggtttgc gaaaggcaga aagcggacat
    17701 atcaagctaa aaggggaaga catcactaat aaaaaacccc gaaaaatcac tgaacatggt
    17761 gtaggacacg tgccagaaga ccgtcataaa tacgggttag tcctagatat gacattatct
    17821 gaaaatatcg cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat
    17881 tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg
    17941 aatgaacttg ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc aattatcgct
    18001 cgtgaagtcg accgagatcc tgatctgttg attgttgcaa atccaactcg tggactggat
    18061 gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac aaagggacaa atacaaagca
    18121 gtgttactga tcagtttcga attagaagaa attttaaatg tttcggatcg tattgctgtt
    18181 atccatgaag gagaaattgt cggtatcgtt gatccgaaag aaacatctga aaatgaatta
    18241 ggtctattga tggcgggcta taccttagaa gaagcacgaa aagagttaga aaagggggct
    18301 ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt
    18361 tgttttagga ttgatcttag gcgcgatatt gatggctgct tttggttttc agcccatcca
    18421 aggttattct tctatgctag atgcagccct tggttcgcaa agagggatag gtgaaacgtt
    18481 aaggcaagca acgccgttga tttttacagc attaggtttt tctgtagcta actcagctgg
    18541 gtttttcaat attgggcttt ctggacaagc actttgcggg tggatcgtca gtgtctgggt
    18601 ggcacttgcg tttccagatc tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg
    18661 tgccttagct ggtgcgatgg cagcggctgt tccaggttta cttcgagcat tttttggaac
    18721 aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa gcacatttgc
    18781 tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg gactcttcca atatgatcag
    18841 tcaaaatgcc tcactgcggt taccatggtt aacacaagct tttggcggct ctcgcgtaaa
    18901 ctcaggatta tttttagcaa tcatcgcgct tattttcgtt tggctgctga tgaagaaaac
    18961 gactttagga tttgaaatcc gttctgttgg attgaatcct tttgcttctg agtatgcagg
    19021 aatgagtagt aaacgtacga tcgttttgtc gatggtcatt tctggcgctt tggcagggct
    19081 tggcggtgtg gttgaaggat taggaacgta tcaaaacttc tttgttcaaa cgacttcgct
    19141 atctattgga tttgacggaa tggctgtttc cttgcttggt tctggatcag caatcggtat
    19201 tcttttatca gcgatcttgt tcagtatttt gaagatcggt ggacttggca tgcagacagg
    19261 agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct ttgattatct tctttgtcgg
    19321 gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa aacaagaaga
    19381 gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac catgaagggg gcgaactata
    19441 atgtcgacta tcgaattaat tgccttgatc cttacttcta cactggttta ttcaacaccg
    19501 ctaattctaa cgtcactagg cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg
    19561 ctagagggaa tcatggtcat gggtgctttt agttccatcg ttttcaactt gacgatgagt
    19621 ccatcacttg gttctatgac accatggatc gctattttag tgggtggcgt cgtaggtatc
    19681 ctattttctc tgattcacgc agtagcaaca gtcagtttgc gtgctgacca tattatcagc
    19741 gggacagtaa ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa agcatggtat
    19801 ggtaaaggtc aaactgataa catcacgcag aatctggggt atttttcttt cccaggatta
    19861 gcagatattc cggtcattgg accaattttc tttaaaaata cattcttacc tgcttattgt
    19921 gctattttga ttgctgtcct agcttgggtg atcatcttca agacgaaatt cggcttgcgc
    19981 ctccgttcag ttggtgaaaa tcctcaagct gccgacacat taggtatcaa tgtatatgct
    20041 atgcgttatt caggcgtact gatttctggc ttgttaggcg gtatgggcgg tgctgttttt
    20101 gctcagtcga tttccggtaa cttttcagct ggtacgatcg ttggacaagg gttcatctcc
    20161 atggctgcta tgatctttgg taagtggaat cctttagggg caatgggtgc ctcattgttc
    20221 tttggttttg ctcaaagctt gagtatcatt ggcgcacaat taccagttat ttcttcgatt
    20281 ccgccagtat tgctgcaaat tgctccatat ttgctgacaa tcattgtgct ggttgttttc
    20341 ttagggaaag cttctggtcc aaaagcaaac gggaagaact atatcaaatc aaaataatct
    20401 tttagcgaat aaaaagggtg ggttacggga agtgtttttc gtctcgccct ttttgtttta
    20461 aaggcaataa aaataaaaag aaagaaggaa taatcatgtt taaacgcgta catctgatcg
    20521 tcatggattc agtaggaatt ggagaagcac cagatgccga taagttcggt gatgttggtt
    20581 ctgatacatt aggacacatt gcgaaagaag caggattgaa cgttccacat ttagagcagc
    20641 ttggtttagg aacgatccgt cctcttgaag gtgtggaatc agtgaaagat catcaaggct
    20701 atgcgacgaa attagaagaa gtttctgtag ggaaagatac gatgacaggc cactgggaaa
    20761 tcatgggatt gaatatcaaa actccatttc gtgtgtttcc taatggattc cctgatgatc
    20821 tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg
    20881 gaacagctgt tattgacgat tacggtgaac accaaatgaa aacaggcgat ttaattgtat
    20941 atacttctgc cgatcctgtc cttcagatcg cggcacatga agaaatcatt cctttagaag
    21001 aattatatcg gatttgccaa ttcgttcgtg atataacaaa agatgatccg tatatgattg
    21061 gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga cttcacacga acaagcaatc
    21121 gccatgatta tgcgttgaat ccattcggac acacagtcct tgattctttg aaagaagcag
    21181 gaaaggacgt gattgctgtt ggaaagatca acgatatttt caacggacaa ggaatcactg
    21241 aatctgtccg cacgaaaagc aatatggatg gcgttgacca gctgctgaaa gttatgaaaa
    21301 acgatttttc aggaatcagc tttaccaatt tagtagattt tgatgcatta tacggtcatc
    21361 gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt tgacttacga attccagaat
    21421 tgcttgaagc gatggaagaa gacgacttgc taatgattac agccgatcac ggaaacgatc
    21481 cgacgtttac tggtaccgat catacgcgag aatatgtacc gttattggtt tacagtaaaa
    21541 aaatgaaaga acaaggggca ttgcctcaag gatattattc agatatttca gcaacgattg
    21601 ctgagaactt tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat
    21661 aatcttttta aataggagaa agcaaatgac caagttaagc gagatgttac aagaaacgac
    21721 ccagtttatc cttgataaag gagtaaaaga aattgatttt ggtttgattc taggttcagg
    21781 attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg atttcttatg atgagatccc
    21841 gttctttcct gtttcgacag tagttgggca tgcaggaaag ctagtttatg ggtctctggc
    21901 aggcaagaaa gtgttagcca tgcaagggag attccactac tatgaaggtc attccatgca
    21961 aacagtgacc tatccagtac gtgtgatggc cggactaggg gcccattcag tcattgtgac
    22021 taatgcatgt ggtggtgtca acgaatcatt tgtaccagga gatttgatgc tgattaccga
    22081 tcatatcaat tttactggtg aaaatccttt gatcggtccg aatgaagatg caatgggccc
    22141 tagatttcca gatatgagtc aagcttatac gcctgactat caagcaacag caaaagcagc
    22201 agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat atgggctatt ctggaccaac
    22261 ttatgaaacg cctgcggaga tccgaatgtc tcggacaata ggagccgatg cagtagggat
    22321 gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc
    22381 ttgtattacc aacttagcag caggtatgca agcgaatctg agccatgaag aagttgtaga
    22441 aacaactcag agagtcaaac aatcttttaa agcattggtt aaagaaacat tgacattatt
    22501 gtaataggag gaacaataaa atgagtgtgc atattgaagc gaaagaaggc gagatcgccg
    22561 ataagatttt acttccggga gatcctttgc gagctaaata tattgcggag acttttttgg
    22621 aagatccagt ttgttacaat caggtacgcg gtatgttagg ctttaccggc aaatataaag
    22681 gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc atctatgccc
    22741 atgaattgat ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat
    22801 tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca aggtgcagcg accagttctt
    22861 cgatgatcga aaaaaatttc caagcctttc attttcctcc aatcagtgat ttcaatctat
    22921 tactgaaagc atatgagata gctaaagaaa agggctatac ggttcatgta ggaaatgttc
    22981 tttcggaaga ttctttctac aaagatgatt tgacagagac gttccaatta gctgaacttg
    23041 gcgttttagg agtggaaatg gaagcagcgg ctctttatta cttaggcgcg aaataccatg
    23101 tgcagacatt aagtttgatg accgttagcg accatttgat caccggtgaa gaaacaacag
    23161 ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa acagcgatag
    23221 cagaataaga aggaaatgaa aaagctgccc tgcattaagt aataagaaca tccaagcaaa
    23281 aatagctcct catgtttttg acttggctgg acttattatt gcaaatgtaa gcttttgaac
    23341 accgtttacc aagaggttgt gaatctgccg tttaactctg agtcacaagg aacaatttca
    23401 aaaaacagct tttcatgttt gatgaatatt gtgacttgtg accgagaagt tggcaggaga
    23461 aaaccgttta tacgaaaaaa gggactgtga caaatcgtgt cacagtccct tttttatagt
    23521 gttttttata ggtagtattt tttagcaact gttaagtcat catttaattc ataaacaagt
    23581 ggttgacctg ttggaatttc aagatccatg atgtcttcgt cagagatacc ttcgatatgt
    23641 ttagctaggg cgcgtaatga gttaccatgt gcagctacta aaacagtttt gttgtctagt
    23701 aatgctggag caatttcatc ttgccagaat ggcaaagcgc gttctaatgt cactttcaag
    23761 ttttctccac caggaatatc gcgtttgtct aacattgcgt aacgacggtc atttgctgca
    23821 gaaccttcgt cagtttcttc cattaatgga ggaagagtat cgtaagaacg acgccagatg
    23881 tgcacttgtt cgtcgccgta tttttcagct gtttcttttt tgttcaaacc ttgtaattta
    23941 ccatagtgac gttcgtttaa gcgccatgat ttgatttgtg gtacccaaag ttgatctgaa
    24001 tattctaaga tcaagttaca tgttttgatt gcacgagtta aaacagaagt gtaagcaaca
    24061 tcaaattcga ttccagcttc tttgattttg cgtccgcctt cttttgcttc ttcgattcct
    24121 tctggtgcta gatttacatc agcccaacca gtaaattggt tcaatgcatt ccattcacta
    24181 agaccgtgac gagaaaaaac taattttggc atgtttagct ctccttttaa gataggttta
    24241 tatttctgaa attttcagtt ctgttttatt gtacaatttt ttggaagaga tttccatcga
    24301 aaaattagat aagattctat gagaaaacaa gtagagaata taaaaaaacg aacagtgccc
    24361 acgcaactgt tcgtttttag gtattagatg cataattgct cgatttcttt ttcaggtaaa
    24421 ttgatatatt tataacatgt tccaactgaa acaccgcatt ttgaagaaat aaattggatc
    24481 gtttcttttt tttcataata gagacgtcgg atttttttga tcgtttttgc atcgattttt
    24541 ggtcttccgc cgatttttcc ttttttacga gcttcatcta atccaactaa tgtacgttct
    24601 ttgatcaatg cgcactccat gtttgctagg ttttccatta attggaaata gatttttccc
    24661 atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg atctttgaat
    24721 aattgcgtga gttctgttaa ttgccgcgta gatttgccta atcgatgaag ttggcaaatg
    24781 actagtgcat cgccttgttg catgtcagcg atcactgact ccaaatgaac agcagtagaa
    24841 tcattttgtt ccacttcata tgtctcatgg aaaatttctt gacaaccaaa gtcagataat
    24901 tgatcaacct gagttgttaa atcaacgtca tttatggttg tgcgtgcata accaataatt
    24961 ttcatggtga ttcctttcta gcagtgattt tctaactgga tataacagta aaaatgtagc
    25021 ataaagtacg tcaaaaagat agttttttcg caaattttcc gctgtctttt tttattggat
    25081 gatcaagcag tttttcttta tttacgacgg attgcgtcaa gaatctcaac gcctttttct
    25141 gagccgacaa ctactttaga aaccatgttt aggaaaagcc catgttctac taccccaaca
    25201 agttcatcta aataggtagc taagctttct ggctgttcga tgacttctag atgcagatca
    25261 atgatgtaat gaccaccatc tgtggttaag acttctccgt tttcgtctgt acgcaagacg
    25321 ggttggaatc ttttttcttc aaaaatatga agtagctgtt gggagccata agggatcact
    25381 tcaactggga gtgggaactt cccaagttta tcgactaatt tagaagaatc aacgatccaa
    25441 atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc gccgccgcct
    25501 ttgatccctt gaaaatcagc actgatttca tcagcaccgt ctattgtcaa atcgactacg
    25561 gggacttcat caatactctt taacggtatt cctaattttt ccgcttgttc ttttgtcgca
    25621 tttgaagtag taactcctgt gatacgtaaa ccttcttcac gcatacggcg accaatctct
    25681 tctaccatat aataggcggt tgaaccggtc ccgagaccta cgatcatccc gtcctttacg
    25741 aatttcgcgg cttcaatgcc agctaattgt tttaaattca tctttgcgtc cacctttttt
    25801 tagtttgagc atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa aagataccat
    25861 tcttttttat tccaataaag ctttttcaaa aaaaattgca tctcttgttg acatgttttt
    25921 tcattcgtga tattctaaca aaggtgcttt atctacaggt ttctgtgaag aaacgtgctg
    25981 actgtttatc aaagcatatt tgataaggat gcagacattg catttatttt ttatcacaaa
    26041 aaaagaacca cctggatgtg tggaactaga cgcaaatcgt gggaaggagg aaacaaggaa
    26101 tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa aaatctaatt
    26161 cacctgcttt gaacaaagga tataacagct ttaagaaaac tcaaactaac gtaaactcac
    26221 ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac acctaaaaaa ccgaactcag
    26281 ctttacgtaa atatgcacgt gttcgtttgt caaacttaat tgaagttact gcttatatcc
    26341 cagggattgg tcataactta caagaacaca gtgttgtatt attacgcggt ggtcgtgtaa
    26401 aagacttacc aggggtacgt taccatatcg ttcgcggtgc tttagatact gctggtgtta
    26461 acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa acctaaagct taattttaat
    26521 tattaaacaa atgaaacttg cttctactat atagaagtaa ttactatgaa caatatttcg
    26581 gaaggaggag ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg ttttaccaga
    26641 tcctatttat aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg ttgacggaaa
    26701 acgcgggatt gcagctaaca ttatctataa tgcctttgac ttgatcaaag aatctacagg
    26761 taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg ttcttgaagt
    26821 taaagcacgt cgtgttgggg gttcaaacta ccaagtacca gttgaagttc gccctgaacg
    26881 ccgcgttact ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg gtgaacacac
    26941 aatggaagaa cgcttagcaa aagaaatcat ggatgctgca aacaacacag gtgcttctgt
    27001 taaaaaacgt gaagacactc ataaaatggc tgatgccaac cgtgcgtttg ctcactatcg
    27061 ctggtaagat tctctctgtt tggtcggctt ttataagtcg acctcagata gtgtccttat
    27121 ttttagcaac tagatagtaa agagaggaga aacaaggaga atgaccagag aattttcttt
    27181 acaaaacact cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa cgactgctac
    27241 tgaacgtatc ttgtactata ctggtaaaat ccataaaatt ggtgaaactc atgaaggagc
    27301 ttcacaaatg gactggatgg aacaagaaca agaacgtggt attacgatca cttctgctgc
    27361 tactacagca caatggaagg gtcaccgaat caacatcatc gacacaccag gacacgtaga
    27421 cttcacagtt gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa ctgttcttga
    27481 tgcacaatca ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa caacttatgg
    27541 tgtacctcgt atcgtatttg caaacaaaat ggacaagatc ggtgctgact tcttatattc
    27601 agtatcaact ttacatgacc gtttgcaagc aaacgctcac ccaatccaat taccaattgg
    27661 tgcggaagac gacttcacag gaattatcga ccttgtaaca atgaaggctg aaatgtatac
    27721 aaacgatctt ggaacagaga tcgaagaaac agaaattcct gaagaatacc gcgaattagc
    27781 tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag aacttacttt
    27841 gaaatactta gaaggtgaag aaatcactga agctgagtta aaagaaggta tccgccgcgc
    27901 aacagttaac gtagaattct acccagtact atgtggttca gcatttaaga acaaaggggt
    27961 tcaattacta ttggacgctg ttcttgatta cttgccatca ccgttagata tcccagctat
    28021 caaagggatt gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg acagcgctcc
    28081 tttctcagct ttagcattta aagttatgac tgacccattc gtaggtcgtt taactttctt
    28141 ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta caaaacgcaa caaaaggcaa
    28201 acgtgaacgt gttggtcgta tcctacagat gcatgccaac tctcgttcag aaatctctga
    28261 agtttacgct ggagatatcg ctgctgctgt tggtttgaaa gacacaacta ctggggacac
    28321 actatgtgat gaaaagagtc tagttattct tgaatccatg gaattcccag aaccagttat
    28381 ccaagtcgct attgaaccta aatcaaaagc tgaccaagat aaaatgggtg tggctttaca
    28441 aaaactatct gaagaagatc ctactttccg tgctgaaaca aacgtggaaa caggcgaaac
    28501 aatcatcgct ggtatgggtg aacttcactt agatatcatc gttgaccgta tgcgtcgtga
    28561 attcaaagtt gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa ctttccgtgc
    28621 tggcactcaa gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc aatacggtca
    28681 cgtatggatt gaatttacac caaatgaaga aggtgctggt ttcgaatttg aaaatgcgat
    28741 cgtcggtggg gttgttcctc gtgaatacat tccagctgtt gaaactggtt tgaaagatgc
    28801 tatggaaaat ggtgtcttag ctggctatcc attagtagat atcaaagcta aactttacga
    28861 tggttcatac catgatgtcg attcaaatga aactgccttc cgtgttgctg cttctatggc
    28921 attacgtgca gcagctaaga aagctaatcc agtaatcctt gaaccaatca tggctgtaga
    28981 agttgttatc cctgaagatt atttaggtga tgttatgggt cacgtaactg ctcgtcgtgg
    29041 acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg cgatggttcc
    29101 acttgctgaa atgtttggtt atgctacaac tcttcgttct gctactcaag gtcgtggtac
    29161 atttacaatg acatttgacc actacgaaga tgtaccaaaa tcagtacaag aagaaatcat
    29221 taagaaaaac ggcggaaaag ctgaataatt tcttgaaacc ggatagtcct gcaaagagat
    29281 tatccaaaga gactatattt ttaatatagt ttcgtgtaaa ataattatcg atgatatggg
    29341 catgtggtgt accttgtgta catcatatac ctatcagaca aataaaaaat atattgtatt
    29401 tttaggagga cattttaaaa tggcaaaaga aaaatttgac cgttctaaac cacacgttaa
    29461 cattggtact atcggacacg ttgaccatgg taaaactaca ttaacagctg caatcacaac
    29521 tgtactatct aagaaaaacg gcggccaagc tatggcttac gatcaaatcg atggtgctcc
    29581 agaagaacgc gaacgcggaa tcacaatctc aacagctcac gttgagtatg aaacagatac
    29641 acgccactac gctcacgttg actgcccagg acacgcggat tacgttaaaa acatgatcac
    29701 tggtgctgct caaatggacg gagctatctt ggtagtttct gctgctgacg gcccaatgcc
    29761 tcaaactcgt gaacacatcc tattgtctcg tcaagttggt gttccttaca tcgttgtatt
    29821 cttgaacaaa gtagacatgg ttgatgacga agaattacta gaattagttg aaatggaagt
    29881 tcgtgaccta ttaacagaat acgaattccc tggtgacgat gttcctgtag ttgctggatc
    29941 agctttgaaa gctctagaag gcgacgcttc atacgaagaa aaaattcttg aattaatggc
    30001 tgcagttgac gaatacatcc caactccaga acgtgacaac gacaaaccat tcatgatgcc
    30061 agttgaagac gtgttctcaa ttactggacg tggtactgtt gctacaggtc gtgttgaacg
    30121 tggacaagtt cgcgttggtg acgaagttga agttgttggt attgctgaag aaacttcaaa
    30181 aacaacagtt actggtgttg aaatgttccg taaattgtta gactacgctg aagctggaga
    30241 caacattggt gctttactac gtggtgttgc acgtgaagac atccaacgtg gacaagtttt
    30301 agctaaacca ggtacaatca cacctcatac aaaattctct gcagaagtat acgtgttgac
    30361 aaaagaagaa ggtggacgtc atactccatt cttcactaac taccgtccac aattctactt
    30421 ccgtacaact gacgtaacag gtgttgttga attaccagaa ggaactgaaa tggttatgcc
    30481 tggcgacaac gtaacaatgg aagttgaatt aatccaccca atcgctatcg aaaacggtac
    30541 taaattctca atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta cagaaatcaa
    30601 agcttaattc acttaagttt taaagtaaag caacatttat tcggacgtaa tcgctcggaa
    30661 cgaaggtctt aggatctttg ttccgagctt ttttatttat tgcaggatca ggaaaaaaga
    30721 caggagtatt ataaatagaa gaaacacgaa ataaaagagt cagtattttc taaatgaaac
    30781 tttatgaaaa caaaatgttt tttacaagaa aaagccttgc tttgtgagtt tatatatatt
    30841 ataatgacaa aggtgcgttt actttagtga attgcacaga cgtaatcaga atgatttcgt
    30901 cggctaagaa acgagaggtt gcgacacgcc cggacgcttt gccacgaacg agcgtgacgg
    30961 aaattttcgt ggagctatgt ctacttttca aaataggcga aggagggaac aagatggcaa
    31021 aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg tattttagat caatcagcgg
    31081 ataaaatcgt ggaaactgca aaaagaactg gagctagcgt gtctggtcca attccattac
    31141 caactgaacg cagtctttac acaatcatcc gtgcgactca taaatacaaa gattcacgcg
    31201 aacaattcga aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca acaccaaaaa
    31261 ctgttgatgc tttgatgaag ctagacttac catctggtgt aaacatcgaa atcaaattat
    31321 aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa aggaatctta gggaaaaaag
    31381 tgggaatgac acaaatcttc actgaatctg gtgaattaat tccagtaact gttgtagaag
    31441 ctactccaaa cgtagtacta caagtgaaaa caatggaaac ggatggctac gaagctattc
    31501 aagttggtta ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa ggtcatgttg
    31561 caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt gagctaggag
    31621 aatatgaagt aggaaaagaa attacagtag atgttttcca agcaggagac attattgatg
    31681 taacaggtac tacgaaaggt aaaggattcc aaggggttat caaacgccac ggacaaagcc
    31741 gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg ggtccagttg
    31801 cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc aaccgcgtaa
    31861 ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc ttgatcaaag
    31921 gtaatgttcc tggagctaaa aaatctttaa ttactatcaa atcagctgtg aaagctaaat
    31981 aattgtagga agaaaggagg aactaaggaa tgccgaatgt agcattattc aaacaagatg
    32041 gaagccaaaa cggtgaaatc acacttaacg aagaaatctt cggaattgaa cctaatgaaa
    32101 gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga acacacgcgg
    32161 ttaaaaaccg tagcgctgtt cgcggcggcg gacgtaaacc atggcgtcaa aaaggaacag
    32221 gtcgtgcacg tcaaggatct atccgctcac cacaatggcg tggaggtggc gtagtctttg
    32281 gaccaacacc acgttcttac agctacaaac ttcctaaaaa agttcgtcgt ttagcaatga
    32341 aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa ggcttgaact
    32401 tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc taacttatct atcgactcta
    32461 aagtattggt tgttctagaa aaaggtaacg actttgctgc tttatcagca cgtaacttac
    32521 caaacgtttc tgtagtaact tctgataatg tgagtgtgct agacgttgta tcaaatacaa
    32581 aagtgttagc aacacaaact gctcttactc aaattgagga ggtgcttgca taatgaactt
    32641 actagacgta atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca tggatgacaa
    32701 gaaatacact tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac aagcggtaga
    32761 agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg cgaaattcaa
    32821 acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg ttacattgac
    32881 tgaagattca aaagaaatcc aattattcga agctgctgaa taagcactaa tagataactc
    32941 attaaattag gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc aaatggtcgt
    33001 cgtaacatga caggttctga ttttgctgaa atcacaacat caacacctga aaaatcattg
    33061 ttacaaccat tgaaaaacca tgctggacgt aacaacaacg gccgcatcac tgtacgtcat
    33121 caaggtggcg gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa caaagataac
    33181 gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc gttcagctaa catcgcgtta
    33241 gtacactatg aagatggagt gaaagcttac atcttagcac ctaaaggact agaagtgggt
    33301 atgaaacttg tttcaggtcc agaagcagat atcaaagtcg gaaatgctct tccattagaa
    33361 aacattccag ttggtactgt tatccacaac attgaaatga aacctggtaa aggtggacaa
    33421 ttgatccgtt ctgctggtac aagtgctcaa gtacttggta aagaaggcaa atacgtattg
    33481 atccgcttga actctggtga agttcgtatg atcttagctg catgccgtgc tacaatcggt
    33541 tctgtaggta acgaacaaca cgaattaatc aacatcggta aagctggacg ctctcgttgg
    33601 atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc ctaatgatca cccacacggt
    33661 ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag tttctccttg gggtcaacca
    33721 gctcttggtt acaaaactcg taacaaaaaa gctaaatcag acaaacttat tgttcgtcgt
    33781 cgcaaaaata aataattact ttgaccatgt gtcgcacatt ttgagaggag gttcaccatg
    33841 ggtcgtagtt taaagaaagg accttttgtc gatgagcatt taatgaaaaa agttgaagct
    33901 caagcagggg ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc aacagttttc
    33961 ccaagttttg ttggatatac catcgcagtt tatgatggac gtaaacatgt accagtttac
    34021 atccaagaag acatggtagg acacaaatta ggtgaatttg cacctacaag aacttatcgt
    34081 ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg aggggaggat aaatccaaat
    34141 ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt cacctcgaaa
    34201 agcccgttta gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa tttcaatctt
    34261 gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt cagcagttgc
    34321 gaatgcagaa aataactttg acttagatgt tgaaaacttg gtagtaagcg aagcttttgt
    34381 taacgaagga cctacaatga aacgtttccg tcctcgtgca aaaggatcag cttctccaat
    34441 caacaaacgt acaagtcata tcacggtagt cgtatcagaa aaataaggag ggataatcag
    34501 tgggtcaaaa agtacatcca attggaatgc gtgtaggcat catccgcgac tgggatgcta
    34561 aatggtatgc tgaaaaagag tatgctgagt tcttacacga agatttaaga atccgtaaat
    34621 ttatttcaac taaacttgct gatgctgctg tatcaacagt tgaaatcgaa cgcgcaacag
    34681 gtcgcgtaaa catttcaatt cacacagcta aaccaggtat ggttattggt aaaggcggtt
    34741 ctgaagtcga aagcctaaga aaagaattaa acaaattaac tggtaaaaga atccatatca
    34801 atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa ggaatcgcac
    34861 gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc caacgctcaa
    34921 tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac ggagcagata
    34981 tcgctcgttc agaaggatac tcagaaggaa ctgttcctct tcatacttta cgtgcggaca
    35041 ttgattacgc atgggaagaa gcagatacaa catacggaaa actaggagtt aaagtgtgga
    35101 tctatcgtgg agaaattctt ccaactaaaa aaaacactga gaaaggaggg aaataatcat
    35161 gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc gcggggaagc
    35221 taaaggcgga aaagaagtag cattcggtga atacggtttg caagctgttg attcacattg
    35281 gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg actcgttaca tgaaacgtgg
    35341 tgggaaagta tggattaaaa ttttccctca caaatcttat actgccaaag caattggggt
    35401 acgtatgggt tctggtaaag gggcacctga aggatgggtt gcaccagtaa aacgtggtaa
    35461 aatcatgttt gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt tacgtctagc
    35521 ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg gtggtgaatc
    35581 gaatgaaggt taaagaaatc agagaattaa ccactgccga aatgcttgat caagaaaaac
    35641 aattaaaaga agaattgttt aatcttagat tccaattagc aacaggtcaa ttagaaaaca
    35701 ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat caaaactgtg ttgcgtgaac
    35761 aagctaagta attatggaag gaggccatta agcgtatgac tgaaggaaga aatcaacgca
    35821 aagtttatca aggtcgcgtg gtttcagaca aaatggataa aacaattacc gttgctgtcg
    35881 aaacaaagaa aaaccaccct atctatggca aacgcatgaa ctattctaag aaatacaaag
    35941 tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt gagaattatg gaaactcgtc
    36001 cattatcagc tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca gttattatct
    36061 aataacacga gattttccta tatagattga atgcaaaatc tgaaaggagg atacacaacg
    36121 tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt gaaattttga
    36181 cgatcaaagt ccttggtgga tctggtcgta aaactgctaa catcggtgat gtgatcgttg
    36241 ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc gttaaagccg
    36301 ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg ttcatatatt aaatttgatg
    36361 aaaatgctgc agtgattatt cgtgatgata aaagccctcg tggaacacgt atctttgggc
    36421 cagttgcacg tgaactacgt gaaaacaact tcatgaagat cgtttcacta gcaccagaag
    36481 ttttataatc ttgagacact tatcaaagga ggtgcgaaac agttatgttt gttaaaaaag
    36541 gcgataaagt taaagttatc actggaaaag acaaaaacaa agaaggcgtt gtactagcag
    36601 cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa aaacaccaaa
    36661 aaccttctca agctgctcct caaggtggaa ttgttgagat ggaagcgcca attcatgttt
    36721 ctaacgtgat ggtggttgac tctactggtg tagctggccg agttggctac aaagaagtag
    36781 acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa taaaagttta
    36841 aggaaggagg gtcttactga atgaaccgcc taaaagaaaa atatcttaaa gaagtaactc
    36901 catcattgat ggaaaaattt aactatagct ctgttatgca aacacctaaa gttgaaaaaa
    36961 tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta gataaagcag
    37021 ttgaagaatt aactttgatc actggtcaaa aaccaatgat tacaaaagct aaaaaatcaa
    37081 tcgctggatt ccgtttacgt gaaggaatgc caattggtgc aaaagttaca ttgcgcggag
    37141 aaagaatgta cgaattctta gacaaattgg tttctgtttc actaccacgt gtacgtgact
    37201 tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta ggtattaaag
    37261 aacaattgat cttcccagaa gtagattatg atttagtaga taaagtacgt ggtatggaca
    37321 tcgttattgt aacaacagca aactctgatg aagaatctcg tgagttgttg acacaattag
    37381 gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa tcaatgattg
    37441 ctaaaaacaa acgtcctgca aaattttcaa cacaagctta tactcgttgc gaacgttgcg
    37501 gacgtccaca ttcagtttat cgtaagttcc atctttgccg tatttgcttc cgcgaacttg
    37561 cctataaagg tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta aattcgcata
    37621 agaaggaggt aaaagatcaa tggtcatgac agatccaatt gcagattttc taactcgtat
    37681 ccgtaacgca aacatggtta aacatgagtc tttagaagta cctgcatcaa aaattaaacg
    37741 tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat acatcgaaga
    37801 tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg aacgtgttat
    37861 cacaaactta aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag ctgacgaagt
    37921 accaaaagta ttgaacggtt taggtatcgc aatcatttcg acttctgaag gtgttgtcac
    37981 tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg tatggtaatc
    38041 aagtagaatc tacaaggagg tgtctttaag tgagccgtat cggaaataaa gtcgttgtta
    38101 tccctgaagg agtaacagtt actcaagacg gaaacaacgt tacagttaaa ggaccaaaag
    38161 gtgaattgac tcgtactttt tcagcagata tcaaaatgaa tatcgaagga aacgaagtaa
    38221 ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca actcgtgcga
    38281 acttcaacaa catggttgta ggtgtcagcg aaggtttcca aaaagcgtta gaactaatcg
    38341 gggttgggta ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt ggttactcac
    38401 atccagtaga aatcacacca ccagctggag taactgttga agtaccagct aacacacaag
    38461 taattgttaa aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac attcgtggaa
    38521 ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta tgttggcgaa tatgtacgcc
    38581 gtaaagaagg taaaactggt aaataatcgt ttagaaaaat tgcatagaag ccagcagcag
    38641 gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac atttttactt gtgccgcagc
    38701 ttctattttt cgaaacgtat ttactgcttg aaatagtagt acaataaaaa taaagaggtg
    38761 acaattgtga ttacaaaacc agataagaac aaaacacgtc aaaagagaca tcgtcgtgta
    38821 cgtaacaata tctctggtac tgctgagcgc ccacgcttga acgtttttcg ttctaacaaa
    38881 aacatctacg ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag tgcctctacc
    38941 ttggataaag aaatttcagg tggaacaaaa acagaagcag cacaagctgt cggtaaacta
    39001 gttgctgaac gtgcagctga aaaaggcatt aaagtagtag tctttgaccg tggtggatac
    39061 ctttaccatg gccgtgtgca agctttagct gaagctgctc gcgaaaatgg actagaattt
    39121 tagaagaagg aggaatacca ttcatggttt atatcgatcc aaaacatttg gaattagaag
    39181 accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt cgtctacgtt
    39241 ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt aggatttggt acaggtaaag
    39301 ctcaagaagt tcctgaagca atccgtaaag caattgaaga tgcgaagaaa aacttagttg
    39361 aagtacctat ggttggttca acaatcccac acgaagtaat cggtgctttt agtggtggta
    39421 gaatcctaat gaaacctgct gtagaaggtt ctggggttgc tgctggtgga ccagttcgtg
    39481 ccgtattaga attagctggg gtagcagata tcacttctaa atctttaggc tcaaatacac
    39541 caatcaacgt tgttcgcgca acagttgaag gtttgaaaca attgaaacgt gcagaagaag
    39601 ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg ataaggagga caacaataat
    39661 ggctgaatta aaagttactt taaaacgcag tattatcgga cgtcctcaaa accaacgcga
    39721 tacagttaaa gcgttaggtc ttacaaagat taacagttca gtggttaagc cttctaatga
    39781 agcaattaaa ggcatgatca atactgtttc tcacctagtg gacgttgaag aaatttaatt
    39841 tagaaaaatc ataactagaa cattaaggag gtgccaaacc tatgaaactt catgaattaa
    39901 aacctgctga aggttcacgc caagtacgta accgtgttgg tcgtggtact tcatctggta
    39961 acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc ggcggtgtgc
    40021 gtttaggatt tgaaggggga caaacaccat tgttccgtcg tttaccaaaa cgaggattca
    40081 caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga tgtattgaac cgctttgaag
    40141 acggagctga agtaactcct gttgctttag ttgaagctgg aatcgtgaaa aacgaaaaag
    40201 ctggaatcaa agtattagct aatggggaac taaacaagaa attaactgtg aaagcagcaa
    40261 aattctctaa agcagctcaa gaagcaattg aagcggctgg tggatcaatc gaggtgatct
    40321 aatgtttaga ctattaaaag atgcttttaa agtaaaagac attagatcga aaatcctatt
    40381 tactgtattt gtgttgtttg tatttcgcct aggtgcacat attactgtac caggcgtaaa
    40441 tgctgcttca ttgcaaaacc tttcttcttt gccatttttc aacatgttga acttggtaag
    40501 tggtagtgcg atgcaaaact tttccatttt ctcaatgggg gtttcaccat acattactgc
    40561 ttctatcgtc attcagcttc ttcaaatgga catcgtgcct cgatttgttg aatggtcgaa
    40621 acaaggggaa gttggtcgta aaaaactgaa tcaagcgact cgatatctga cattagtatt
    40681 ggcatttatc caatctgtcg gtatcactgc tggattcaat caatacgctc aattaggttt
    40741 cgtgaataat cctaacgtag caacttacgt aacgatcggt ttgattctaa cagccggtac
    40801 gatgtttgtt acttggctag gagaacagat cacagaaaaa ggtatcggaa atggcgtatc
    40861 aatgatcatt tttgcaggta tcatttctag attgcctgaa agtatcagag aaatttatga
    40921 agattacttt ataaatattg atccttcaga catatggaaa tctgctattt ttgtggccat
    40981 tttactagta gcggtcttgt tgatcgtgac attcgtaaca tttttccaac aagctgaacg
    41041 caagatcccg atccaatata cgaaacgcgt agctggcgcg cctacaagca gctatctgcc
    41101 gttgaaagta aatgcagctg gagttattcc agttatcttt gctagttcgt ttattgctac
    41161 gccaaatgct gttttacaag cattgagcgg tagattcggt tctgaaaact ggtacaaagt
    41221 gatgatggag atctttaact ataatacagc acctggtgct acgatttata cgattctgat
    41281 cgttgccttc actttcttct acgcctttgt tcaagtaaac cctgagaaat tagcggaaaa
    41341 cttgcaaaag caaggaagtt atataccaag cgtacgccct ggtaagggga ctgaagagta
    41401 tgtctcaaga ttactgatgc gtttaagtac agttggtgca gtcttccttg gtttagtggc
    41461 attgcttcca atcattgctc aaatggtttg ggatcttcca caatcaatcg gattaggcgg
    41521 tacaagctta ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt tagaaggctt
    41581 aatgctgaaa cgtaaatatg ttggattcat tgagtaagaa agcgtgttga ggtttcccct
    41641 caacacacgt tctcacacat ttattaggag ggaaaacaat gaacctcatt ttaatggggc
    41701 tgccaggcgc aggtaaagga acacaagctg aaaaaatcat tgatgcttat ggtattccgc
    41761 acatctcaac aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct cttggcctgg
    41821 aagcaaagtc ttatatggat aaaggtgcat tagttcctga tgaagtaaca aatggaatcg
    41881 tgaaagagcg actagctgaa ccagatacag agaaaggctt cttattagat ggctttccac
    41941 gtacgctaga tcaagcaaaa gctctggacg caatgctaaa agatttgaat aaaaaaattg
    42001 atgctgtcat cgatatccat gttggtgaag aaatcttggt cgaacgtcta gccggccgat
    42061 ttatctgcag caattgtgga gcaacttacc ataaaatttt caaccctaca aaagttgaag
    42121 acacatgtga tcgttgcggt gggcatgaat tctatcaaag agaagatgat aaacctgaga
    42181 cggttaaaaa tcgtctggct atcaacatca aaaatagtga accaatcttg gcttattata
    42241 aagaacaagg tttgctaaac actattgatg gtgctcgtga aatcgatgct gtatttgcag
    42301 atgtacaaaa aatcattgaa aaatagcacg attgcccgta gccgacttgc attatgcagt
    42361 tactcttgtc aagttcaata aattatgata gaatatgtca gtccgacttc tagagaatcc
    42421 tctagatgat ccattttgag aattttgagg tgggaactgc agtttccgcc attttatcgt
    42481 gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga tatgattgaa
    42541 gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt ttaaagttga actagaaaac
    42601 ggacaccaag ttcttgctac tgtttcaggt aaaattcgta tgcattacat ccgtatctta
    42661 cctggtgaca aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg ccgtattact
    42721 tatcgcttta aataattgta ctccgtaaaa ctacaggagg tattatcatg aaagtaagac
    42781 catcagtaaa accaatgtgc gaacattgta aagtaattcg tcgtaaagga cgcgttatgg
    42841 tgatttgccc agcaaatcca aaacataaac aacgtcaagg ataattggag gtgctataaa
    42901 tatggctcgt attgcaggag tagatattcc tcgtgataaa cgtgtagtag tttcacttac
    42961 ttatatctat ggtattggta acactactgc taaaaagatc ttagctgatg ctggtgtatc
    43021 tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc gtgcagaagt
    43081 agataaatta aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca tcaaacgttt
    43141 gatggaaatc ggttcttacc gtggaatccg tcatcgccgt ggattgcctg ttcgtggaca
    43201 aaacacgaaa aacaatgcac gtactcgtaa aggcccagct cgcgctatcg ctggcaagaa
    43261 aaaataattc ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag tgaatcgtaa
    43321 acgccgtgtg aagaagaata tagaagcagg tattgcacat atccattcta cattcaataa
    43381 cactatcatc atgatcactg atactcacgg aaatgcatta gcgtggtcat cagctggttc
    43441 attaggtttc aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg cagcagaaac
    43501 tgctacaaaa gcagcaatgg aacacggatt aaagactgtt gaagtaacag ttaaaggacc
    43561 tggttctgga cgtgaagcag caattcgttc attacaagct acaggtttag aagtgactgc
    43621 aattcgtgac gtgactccag ttcctcataa tggtgcccgc cctccaaaac gccgtcgtgt
    43681 ttaatgagtg ccgctcattt tgagttttat cagtaacgtc attgaacaag acacttttcg
    43741 ttttgaaagg ggtaaagata agaatgattg agttcgaaaa accaagaatc gcaaaaattg
    43801 atgaagaaaa agattatggc aagttcatcg ttgaacctct tgaaagaggg tatgggacta
    43861 ctttagggaa ttccctacgt cgtattttat tatcttcttt gccaggtgct gccatcacta
    43921 atattcaaat cgatggtgta ctgcatgaat tctctaccat caaaggtgtt agagaagacg
    43981 tcactcaaat catcttgaac attaaaggct tagcgctgaa aatgtatggc caagaagaaa
    44041 aaacccttga aatcgatatt accggacctg ctacagtaac tgctggcgat attatcgttg
    44101 atagtgatgt agaaattctt aacaaagata tgtacatctg tagtgtctct gaaggagcta
    44161 cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca gatgaaaata
    44221 aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc aatctacaca cctgttcgtc
    44281 gtgtgaacta tcaagttgag aacacacgtg ttggacaccg tgaagatttt gataaattga
    44341 cgatggagat ttggacagat ggttcaattg aaccgttgga agcaatgagt ttgtctgcga
    44401 aaatcatgac tgaacatttg gatatttttg tgaacttaac tgatgaagca aaaaatgctg
    44461 aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg acgatcgaag
    44521 agttagactt gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc aatactgtac
    44581 aagaacttac aaataaatct gagcctgaaa tgatcaaagt ccgtaactta ggacgtaaat
    44641 cattagaaga agttaaagca aaattgcacg acttaggttt aggtttacgt aaagaagatt
    44701 aaaacctttt acgaaggagg gaaaccaacg tgagttatcg taaactagga cgcacatcta
    44761 gccaacgtaa agcgatgttg cgtgatttaa caactgattt attaatcaac gaacgtattg
    44821 tgactactga agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg atcactttag
    44881 gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt aatgaagttg
    44941 ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc agctttacaa aaactgttca
    45001 acgatattgc acctcgttac gcagaacgtc aaggcggata cacacgcatt ttgaagacag
    45061 aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa aatcacacta
    45121 ataacatcac tttatagatg attcttttag agtgttatga tgttggaaga tatttttccg
    45181 agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca ctcatcataa
    45241 agtgtttttt tataccttca attacctgtc atgacagata tgtttgaagg cataaaaaaa
    45301 cattttaagc gagaaataat tgaaaatttt gtcttttttt gatagtgtta gtaggaactt
    45361 taggatgaag aaggagtaat catggagcca attattgaat tagaaaagat aaattataaa
    45421 taccagccag atgatcttcg ccccgcattg aaagatgtct cctttacaat tgataaaggt
    45481 gagtggatcg ctattattgg acacaatggt tcaggaaaat cgacacttgc caaaacgatc
    45541 aatggattgc ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat attggatgaa
    45601 gaaaatattt ggacgatccg acagatggtt ggaatggttt ttcaaaatcc agacaatcag
    45661 tttgttggtt cgacagtaga agatgacgtt gcttttggtc ttgaaaatca agggattccc
    45721 agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga aagttagaat ggctgagttt
    45781 gcttcacgcg agcctgctcg tttatcagga gggcaaaaac aacgtgttgc cattgccggc
    45841 gtggttgcct tacgacctga tatcattatt ttagatgaag caaccagtat gctagatcca
    45901 gaaggtcgtg aagaagtgat ctctacgatc aagaagatca aagaagaaag tcaattgacc
    45961 gttatatcta tcactcatga tattgatgaa gcagctaatg ccaatcggat actagtgatg
    46021 agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc tggtcctgaa
    46081 ctgatcgact taggtctaga tctaccattt cctgaaaaac tgaagagcgc actgaaagaa
    46141 cgtggggttg atgtgcccag tgaatatatg acagaagaaa ggatggtgga ttggttatgg
    46201 acatccgttt tgaacaagta gacttcactt atcagccgaa tacaccattt gaacaaagag
    46261 ccttatttga catcaatatg acgatcaaag aaaacagtta tactgcatta gtagggcata
    46321 ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc gcttgttaag ccaacaagcg
    46381 gtacagtcca tattggagaa cgagatattc agccggatac ggataataag aatttaaagc
    46441 ccatccgaaa aaaagttggc atcgtctttc agtttccaga agcacagcta ttcgaagaaa
    46501 cggtagcaaa agacattgct ttcggtccta aaaactttgg tgtcagcgaa gaagaagcac
    46561 tagtcctagc aaaagaaaca ttagaacaag ttgggctgga tgaaagctat ttggaacgtt
    46621 cgccatttga actttcagga gggcaaatgc gtagagtagc aatcgctggt gtgcttgcca
    46681 tgaggccgga agtacttgta ttggatgaac caacggcagg acttgatccc cagggcagaa
    46741 aagaaatgat ggagatgttt tggcgtcttc ataaggaaca gcagatcact attgtattag
    46801 ttacgcattt aatggatgat gtagccaatt ttgcagatta tgtctatgtg ttggaaaagg
    46861 gacgaatcgt taatagcggg gagccgcaag aggtctttca aaacattgaa tggctaaaag
    46921 aaaaacaact aggcgtacct actgccaccg agtttgcaga agaattaatg gctaaaggga
    46981 tgaactttgc taccttgcct ttgacagctg aagaattagc agacgctatc gtccattatg
    47041 ctggaggaaa tgcccatgat gaataaattg atatttggtc gttatatgcc tggagattcc
    47101 ttgatccatc gattggatcc ccgagctaaa ttattagcca gtttttattt tatcggaatc
    47161 atttttttag caaataattg ggaaacgtac cttttgttgg catttttcac attactttct
    47221 gtcttcttgt caaaagtaga tttgaaattt tttatccgag gcattcgccc attgatctgg
    47281 ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga aacttattgg
    47341 agttggggtg ttttccatat cactgaattt ggtgtgatga acggcctctt tattttctgt
    47401 cgattcgtgt tgatcatttt tatgtcgact ctactcacgc ttactacacc gcctttggaa
    47461 ttatcggatg cgattgagta cttgcttcgt ccattgaaag ccgtacggtt cccagtccac
    47521 gaaatttcat tgatgctgtc gattgctctt cgttttgtgc ctacattgat ggacgaaaca
    47581 gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg aaacctagtt
    47641 cagaaaatga aagcagtcat tcctttgctg attcctttat ttgtcagcag cttcaaccgt
    47701 gcggaagatc ttgcgactgc tatggaagca agaggctaca aaggtgggga aggaagaacg
    47761 aaatatcgtg tattgcattg gcataataga gacacgatgg ttatcattgc ttttgtatta
    47821 ttaactgtgc tactgatctt tttaagagga taaacgttca gtgggccgct atctattctt
    47881 ttcttgctga tctgtttgca agaaaggatg caagcagtgt atgacaagaa atattagggt
    47941 ctgtggcaga ctaaggtcac aggcctcttt cttttatctg aaaagaaaga acgaaaaa
//
LOCUS       DAZKNM010000005        47629 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000005 DAZKNM010000000
VERSION     DAZKNM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.005"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1035
                     /locus_tag="ACG8M9_000215"
     CDS             <1..1035
                     /locus_tag="ACG8M9_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0377021.1"
                     /translation="DQKRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNE
                     KNRNESTTSSQLEEWLIGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNS
                     THSVISAHSGLPNRRLFTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTI
                     SEGEDLVTLLTCTPYMINTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTI
                     IAVVILLVFIGRLIYQYRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVP
                     FSVQSDHHGKVKLDQLPGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQ
                     KNGFYFKINE"
     gene            1051..1494
                     /locus_tag="ACG8M9_000216"
     CDS             1051..1494
                     /locus_tag="ACG8M9_000216"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0377022.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(1977..2507)
                     /locus_tag="ACG8M9_000217"
     CDS             complement(1977..2507)
                     /locus_tag="ACG8M9_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0377023.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            2612..3163
                     /locus_tag="ACG8M9_000218"
     CDS             2612..3163
                     /locus_tag="ACG8M9_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0377024.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(3316..3789)
                     /locus_tag="ACG8M9_000219"
     CDS             complement(3316..3789)
                     /locus_tag="ACG8M9_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0377025.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            3927..5399
                     /locus_tag="ACG8M9_000220"
     CDS             3927..5399
                     /locus_tag="ACG8M9_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0377026.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            5396..6106
                     /locus_tag="ACG8M9_000221"
     CDS             5396..6106
                     /locus_tag="ACG8M9_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0377027.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            6458..6742
                     /locus_tag="ACG8M9_000222"
     CDS             6458..6742
                     /locus_tag="ACG8M9_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0377028.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(7023..7442)
                     /locus_tag="ACG8M9_000223"
     CDS             complement(7023..7442)
                     /locus_tag="ACG8M9_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0377029.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(7444..8028)
                     /locus_tag="ACG8M9_000224"
     CDS             complement(7444..8028)
                     /locus_tag="ACG8M9_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0377030.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(8075..9550)
                     /locus_tag="ACG8M9_000225"
     CDS             complement(8075..9550)
                     /locus_tag="ACG8M9_000225"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0377031.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            9731..10042
                     /locus_tag="ACG8M9_000226"
     CDS             9731..10042
                     /locus_tag="ACG8M9_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377032.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(10157..12493)
                     /locus_tag="ACG8M9_000227"
     CDS             complement(10157..12493)
                     /locus_tag="ACG8M9_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0377033.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            12635..13303
                     /locus_tag="ACG8M9_000228"
     CDS             12635..13303
                     /locus_tag="ACG8M9_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0377034.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            13306..14349
                     /locus_tag="ACG8M9_000229"
     CDS             13306..14349
                     /locus_tag="ACG8M9_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0377035.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(14586..15467)
                     /locus_tag="ACG8M9_000230"
     CDS             complement(14586..15467)
                     /locus_tag="ACG8M9_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0377036.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            15626..16354
                     /locus_tag="ACG8M9_000231"
     CDS             15626..16354
                     /locus_tag="ACG8M9_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0377037.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            16510..16827
                     /locus_tag="ACG8M9_000232"
     CDS             16510..16827
                     /locus_tag="ACG8M9_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0377038.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            16820..17428
                     /locus_tag="ACG8M9_000233"
     CDS             16820..17428
                     /locus_tag="ACG8M9_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0377039.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            17483..18274
                     /locus_tag="ACG8M9_000234"
     CDS             17483..18274
                     /locus_tag="ACG8M9_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0377040.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            18412..18900
                     /locus_tag="ACG8M9_000235"
     CDS             18412..18900
                     /locus_tag="ACG8M9_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0377041.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            18951..19823
                     /locus_tag="ACG8M9_000236"
     CDS             18951..19823
                     /locus_tag="ACG8M9_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0377042.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            19999..20361
                     /locus_tag="ACG8M9_000237"
     CDS             19999..20361
                     /locus_tag="ACG8M9_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0377043.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            20370..21122
                     /locus_tag="ACG8M9_000238"
     CDS             20370..21122
                     /locus_tag="ACG8M9_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377044.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            21136..22938
                     /locus_tag="ACG8M9_000239"
     CDS             21136..22938
                     /locus_tag="ACG8M9_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377045.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            23215..24255
                     /locus_tag="ACG8M9_000240"
     CDS             23215..24255
                     /locus_tag="ACG8M9_000240"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0377046.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24307..26451)
                     /locus_tag="ACG8M9_000241"
     CDS             complement(24307..26451)
                     /locus_tag="ACG8M9_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0377047.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(26808..28181)
                     /locus_tag="ACG8M9_000242"
     CDS             complement(26808..28181)
                     /locus_tag="ACG8M9_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0377048.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(28181..28576)
                     /gene="rbsD"
                     /locus_tag="ACG8M9_000243"
     CDS             complement(28181..28576)
                     /gene="rbsD"
                     /locus_tag="ACG8M9_000243"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0377049.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(28604..29521)
                     /gene="rbsK"
                     /locus_tag="ACG8M9_000244"
     CDS             complement(28604..29521)
                     /gene="rbsK"
                     /locus_tag="ACG8M9_000244"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0377050.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(29629..30642)
                     /locus_tag="ACG8M9_000245"
     CDS             complement(29629..30642)
                     /locus_tag="ACG8M9_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0377051.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(30723..31322)
                     /locus_tag="ACG8M9_000246"
     CDS             complement(30723..31322)
                     /locus_tag="ACG8M9_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0377052.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            31508..32209
                     /locus_tag="ACG8M9_000247"
     CDS             31508..32209
                     /locus_tag="ACG8M9_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377053.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            32219..34930
                     /locus_tag="ACG8M9_000248"
     CDS             32219..34930
                     /locus_tag="ACG8M9_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0377054.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            35381..37009
                     /gene="treC"
                     /locus_tag="ACG8M9_000249"
     CDS             35381..37009
                     /gene="treC"
                     /locus_tag="ACG8M9_000249"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0377055.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(37069..38250)
                     /locus_tag="ACG8M9_000250"
     CDS             complement(37069..38250)
                     /locus_tag="ACG8M9_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0377056.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            38502..40025
                     /locus_tag="ACG8M9_000251"
     CDS             38502..40025
                     /locus_tag="ACG8M9_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0377057.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(40094..40822)
                     /locus_tag="ACG8M9_000252"
     CDS             complement(40094..40822)
                     /locus_tag="ACG8M9_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0377058.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(40835..42115)
                     /locus_tag="ACG8M9_000253"
     CDS             complement(40835..42115)
                     /locus_tag="ACG8M9_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0377059.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(42256..42858)
                     /locus_tag="ACG8M9_000254"
     CDS             complement(42256..42858)
                     /locus_tag="ACG8M9_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0377060.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            43103..43309
                     /locus_tag="ACG8M9_000255"
     CDS             43103..43309
                     /locus_tag="ACG8M9_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377061.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            43621..44340
                     /locus_tag="ACG8M9_000256"
     CDS             43621..44340
                     /locus_tag="ACG8M9_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0377062.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(44421..45104)
                     /locus_tag="ACG8M9_000257"
     CDS             complement(44421..45104)
                     /locus_tag="ACG8M9_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0377063.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            45322..47508
                     /locus_tag="ACG8M9_000258"
     CDS             45322..47508
                     /locus_tag="ACG8M9_000258"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0377064.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        15454 a         8441 c         9525 g        14209 t
ORIGIN      
        1 gatcaaaaac gtatcgaaca agtacaaaaa cggaccgcag ctgaaattga agtgcagcga
       61 aagaaaatgg aggagaaaaa ccaacgatta actgaccaag gattcaatcc tggtactgat
      121 cctttcaatg aaaagaacag aaatgaaagt acaacatcca gccaattaga ggaatggctg
      181 atcggaagta tcaatatacc taagatccaa ttaaatattt cactttatga tcggctcaat
      241 ggtaggatcc ttgaaaatgg agcaggcgtc ttgcaaggaa cctctttccc gcttggtgga
      301 aattcgacgc attcagtgat ctctgctcac agcggtttac ctaatcgacg gttatttacc
      361 gaattagatc gtttagaaaa cggagacacg tttattttga ctgttttggg ggagaaattg
      421 gcttatcaag ttaaaaatat acaagtggta ctgccagatg acacgtctgt tctaacgatt
      481 tcagaaggag aagacttagt taccttgctt acatgtacgc cgtatatgat caacacgcat
      541 cgtttgctag tgacaggaca tcggattcct tataacgaat cggtaaaaaa agaagaagga
      601 aaaggaaatc aggaaagaac catgcggcaa tggtttattc ttgcaggaac gataatagct
      661 gtagtcatac tgttagtgtt tatcggacga ctaatctatc agtatagatt aagtaaaaaa
      721 gtgcttgatt tttcatttat tattagtgat tcgtcaggga aaccggtaaa tggtagttca
      781 ttcattttga aacataagaa aaaaacactt actagaaatg gtgtgccgtt ttcagtacaa
      841 agcgaccatc atgggaaagt caagctggat caattgcctg gtggaactta tcgaatcgtt
      901 tcagctgatg atcctaaagt tgctgtttct tttggtatta gaaaagtaaa acaagagaag
      961 atatactttt ttgaggggcg aaaacttgta aaagagcttc aaaaaaatgg tttttatttt
     1021 aaaatcaatg aataagtaag gagagaaaaa atgatccgca caacaacaaa attgtctgcc
     1081 aaagaactgg aatctgttct atcgatttgg ctgcatgcta acttggattc tcatgaattc
     1141 attccatcaa cttattggga gaatcaagta gaatctgtta ggaaagcttt accagatgca
     1201 gtgatttact tagcagaaat agatcaagaa attattggtt ttgctggggt gattgatcag
     1261 tttatcgcag gaatctttgt gaaaagggaa taccggcaac aaagaatcgg tacgcaacta
     1321 atgaaaaaaa taaaagaaac caataaaaca ctgactttga cagtctatca aaaaaatcaa
     1381 tcagctattc atttctatct ttcacaaggt tttacaattc aaacacagca aaaagatgaa
     1441 gcagtaaatg aggaggaact tgtgatgaaa tggaatcaaa acgctgattt ttgatgaaca
     1501 gaataacgaa ataatgctcc cagcctaatt ttttgctggg agcattattt cattttgaag
     1561 tcactaattt aactgtgtga atatgcagtt gttacaatct ttgataaacg gtgttcaatc
     1621 agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt tttccatttg
     1681 tttctctaat actcaggtca aaacgctgat ttcacaacct cttgataaac ggtgttcaaa
     1741 cggctgcttc tcggtaataa gcccactagc ctagaaaata tgaaaagcta ttttctaact
     1801 agcgttctta ttactcaaag cataacgctg atttcacaac ctcttgataa acggtgttca
     1861 atcagctgac cctcggtatt agctcaaaaa acggcaaaac atgaggaact atttttccgt
     1921 ttctttctct aatactcagg tcagaacgct gatttcacaa cctctctttc acaaccttat
     1981 tttactcgtt tataattttc atcgaagtat tttccgctac ggatatcatc tggcattcca
     2041 tcgtatggtt cttcgctaat aacatcttca ttgtctatac ctgcatcacc catataagtg
     2101 atcgtagcaa attcaggaac aagtaatttt ggataagttt catatttttc acgagctgct
     2161 tccatttctg cgatatgttc gttttggaaa gtaacagtga tttctggatg ttcttcaacc
     2221 catttaggga aaaagatcat tccgtgaagt ttcttttcat ttggcataaa gtccaatgcc
     2281 acgtctgtcc ctgttggctc tgtccaagtg attttgaata cgccgtccgt caatttaacg
     2341 atatcagctt cttggtcttt tacccaacgg cctttgacca ttccgccatg gatacggtaa
     2401 tcaacagtat ggtcattttt ggcataccat tcatattccc aaccattgtc atacgtatag
     2461 ataaagtgag ttccaataaa atcttccaat tctttaaatt ctttcatgtt taagcatctc
     2521 cttataaatt aaaacatgta ataaactaca tgttttcgat tactcattta tgatagaatg
     2581 gatttaaaga tttgtcaagg agggaaaact gatggcaaga aaaaatacac ttcgatatat
     2641 tttgttaggg ttattaagta agaagaagat gacaggatat gaattgaatc aatcgttcaa
     2701 aaatgaaata ggagaatttt ggcaagcaaa acatagtcag atttatcctg aacttgcaaa
     2761 aatggaagag caaggaatca ttcagcatca agtagagatc acaggtgaaa aattggaaaa
     2821 gaaagtttat gaattaacag aagaagggaa ggaacaattg gcagagtgga ttcatacacc
     2881 caccaatgaa ctaccggtta atcgagacga atttgttctg aaattgtatt tcgtcaaaga
     2941 cgtgaatgat ccggcattag tagaaattat tcagcagcag cgtgaccttc atgaagaaaa
     3001 acgacttcat ctattagagc gaaaaaaaac gatctttcct actgaaaaag aaaaacatga
     3061 aaattacgga cactacctga ttttagcgca tgcgatcaat cgggaaacag aatatgcaaa
     3121 ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa taaaaaaata tccgaacagt
     3181 gatggagaat tcctttctat atgaaagaaa cgcagatcac cgttcggata ttcttatgtt
     3241 caaacaggcc ttttttcaag tatttatgta gcatctactt attaaaaagc ttggtactta
     3301 ggagattagt ggcgtttaga gttcttttaa caactgttcg tagtcagctt tttcatcgac
     3361 aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca tgaatgatgt acatcttagc
     3421 attctcttct ttgaagttac cgatttgtaa aatcgtatag cgttcgtcga tcaattcgat
     3481 atctttgatt gcttttgaaa aaactttacg gacatggtgc attttctttt ggcggatttc
     3541 tgccttttct gcagcttttg acaaagtaaa tccgtcatct aaatattttt tgatcagttc
     3601 tacttttatg actgtaggta aacgatattt tctaggacaa ttcgcatcag cttcgatcga
     3661 acgaatgaat cccttttgtt cccaatacct taattgacgt ggagacacac ctacgatatc
     3721 acttaattca ctgatgccaa caaccaattg atcattttcc aacaaacgtt taagggaagc
     3781 cccaaccatt tttgtcactc cttttgtcct cttgcttccc ttaattatat ccgtattaac
     3841 ttatgtgtca agtaggttga caaatagcga atttaggact aaagtattct agtagtttca
     3901 gaaaaaattc agaaagaagg agaaatatga gtaacaacca acccgtagat atttacggaa
     3961 aaccatataa tcggacactt ttagttgttg tcttattaat tggtacattt tgtaccgttt
     4021 tgaatcagac cttattgacg acagcttttc ctacattgat gaaagctttc gatatttcag
     4081 cgtctgatgt gcaatggtta acgactggtt tcttgctagt caacgggatc atgatcccaa
     4141 taacagcatg gctgatcaat aaattcagtt caagaaattt atatctttct gccatgacga
     4201 tctttttgat cggaacgatt acttgcttta cagcacctaa ttttagtaca ttactaatcg
     4261 gtcgtttgat ccaagcttgc ggtgtgggag tatcgatgcc attattgcaa aatatcatgc
     4321 tatctatttt cccgccagaa aaaagaggat cagcaatggg gatgtcaggg atcgttattg
     4381 gacttgctcc tgctttaggc cctacattat ctggttatat cattgataac taccattggc
     4441 gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt cttggtttta gcattcttcc
     4501 taatgaaaag tgttattcca ttgtcaaatc ctagcattga tattttatcc gctattttat
     4561 caacgattgg attcggtagt ttactttacg gattttctag tgtaggagat gacggctggg
     4621 gaagcttgaa agtaattggt ttcttagttg ttggtgtgat tttcatcgcc ttgttctcat
     4681 ggcgtcaatt acatttggaa catccattct tggaacttcg tgtattcaaa tcatctacat
     4741 tcacgattgc agctgtttta gctggtgtaa caaatatggc gatggtaggg gcagaaatgg
     4801 tcgtaccatt gtatattcaa aatattcgtg gagaatcagc attccattct ggcttgatgt
     4861 tgttgccggg tgcattgatc atgggggcta tgatgcctgt gacgggtgcg attttcgata
     4921 aatacggcgc gaaacgttta gccatcacag ggatgttcat tttgacagcg gcaactgcac
     4981 catttgcttt cttgacaaaa acgactccag ttttatatat cgtgatcctt tatgcgatcc
     5041 gtatgtttgg gatctccatg gtcatgatgc cggtaacgac ttctggtatg aatgcattgc
     5101 caaataactt gatcagtcat gggacagcgg taaataatac attccgtcaa atcgcaagct
     5161 cgattggtac agctattttg atcagtgtgt tgacgaatgt gacaaaagca aatatcccaa
     5221 gcaaatctgt attggataat acgccattag cttataaaga tcaggcaatc aatgccacac
     5281 tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt tgggattctt ggctttatta
     5341 ttgccttttt attgaagaaa aaagaacctg ttctaatgaa agcgggggat aatgcatgat
     5401 tattggagtt ttgattgtca gcttgcttgc ttttgcttta acgaacgtgt tcgctaaaaa
     5461 aacatggcaa acctttttat ctttgatttt cggactaatc ttcgtcgctt cactaagttt
     5521 gatcgtcgct aatttgtcca atcactttgg aatggaaaaa gtaacggaaa caaaaacaga
     5581 aaaaattgtt tccagtgctg attcccaagg agcagatatg cttttgtata aagctttagg
     5641 gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa aaacaagaaa aaccaaaagc
     5701 aactggtacg gataacgaaa caaataaagt agaaaagaca gacggcgatg ctgaaaaagt
     5761 gacgaaaact acttattggg aatacaaaaa cgacatgtat aaattctggt tcaatattgc
     5821 tgataacaat cacgaatatg acagccgagt gaatacattc aaaatcccag agacatgggt
     5881 agaattaagt acggaccaag cagccaagtt agctgaatta gtgaagaaac agcaaagtac
     5941 gatggaaagt gaagcaaaag cttatgttca agacggtatg gtaaaagcaa tgactgaaaa
     6001 tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa gccttagctg ctgaattcca
     6061 acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc gaataatatt ttgaacacca
     6121 tgtaggaggt tgtgaaaaag ctgctctgca tcaagcagta agaacaatca gataaaaata
     6181 gcactccata tttttcatct gattgggctt agtgccgtag atgcaagctt ttgaacaccg
     6241 tttaaggaag aggttgtgag cctgccgttt aactctgagt cacaaggagc aatttcaaaa
     6301 aacagcttct catgtttgtt gactattgtg acttgtgacc gaggagttgg caggagaaca
     6361 ccgtttattc gaaaaaagga actgcgacaa gacttttgtc acagttcctt cttttttttg
     6421 ctatgctata ctagatgtaa aaaataggag aaataaaatg agggaaattt attcttttga
     6481 atcaaaaatc tatgcttccg aagtcggcaa gggcggggca tatattattt ttccgtatga
     6541 tataagaaaa gaattcggtg ctggaagagt taaagtccaa gcaacttttg acaatgtgcc
     6601 gtatgaaggc agtatcgtga atatgggcgt gaaaaatcca gatggttcag tctgctacat
     6661 ccttggacta cgaaaagata ttcgtaaaaa catcggaaaa gaaatcggtg atattgtagc
     6721 agttacagtg aagcaaaaat aaaagaggtt gtgacaaaag acgtgtcaca acctcttgat
     6781 aaacggtgtt caaaagccag tttctcggta gtaagtcaaa tttatgcaaa aatgtggaaa
     6841 cccatgttta caaaattttt cttactactc gaaacgaagc ggctttatca caacctcttg
     6901 ataaacggcg ttcaatcagc tgtccctcgg tattagctca aaaacggcaa aatatgagaa
     6961 gctatttttc catttttttc tctcatactc aggtcataac gctgatttca caacctcttc
     7021 tttcataatg gtttttcacc atctttataa taaactggtt ctttgatatg gaaatctatt
     7081 ttttccaacc aatctaaaat cttgtaacct aactcttctg acatcggaaa agaggcgttg
     7141 atttgtgcat tggagatatg gtcaacatgg ataccggaag tgatcgtaca acttcccgga
     7201 acggcagttt tgatgatcac agctattttt tcggctaaga catcgtcttt atggaaccga
     7261 ccatcgtggc tagggaaacg tacagtttgc atctctgtat cacgagaaaa tgtagtgact
     7321 gttccgatat gtggtgtatc tccgcctgtt aaagtgatca ataagtcttt accgattact
     7381 ttaacagcag cttggatcgt gaaacctagt ttggttactt caactatcgt ttctttttcc
     7441 atattaatca ccctgccaac gttttgaatg ctcatttaca atttcaaatt gatccaaaag
     7501 tttcattgta tggtgattaa caagatcttg gatcgtttga ggctgattat aaaaagcagg
     7561 aattggtggt atgatttgca cacctagtcg tgctaattta gtcaagtttt ccaagtgaat
     7621 ggcatttaat ggcgtttcac gaggaacaat gactaacttt cgttgctcct tcattgttac
     7681 atcagcagca cgtgcaatca ggttgtcgcc taaaccaaca gagatacctg cgactgtttt
     7741 catacttgcc ggtacgatga tcattccatc ggtcaaaaat gatccgctgg cgacagcagc
     7801 acctaaatct ttgtttgaat aatggtaatc aaacaggctt tcgaattcag ttagtgataa
     7861 gtcggattcc agctttaagt tctgttttgc ccatggactg atgattccgt gtgtttctat
     7921 catcggatat tcttttagtt tcttcactag gttgatggca taaatggtgc ctgaagcgcc
     7981 acttacacca atcacgattt ttttcttccg ttgtgccaat tgttccattt tccgcactcc
     8041 tttttatgat gtcaaaacag ttgcagatca ttcattattt caaatatttt tcccaatctt
     8101 ctacttcttg gaattttgca cgtacaaaat gttctttcat atcgaaagga actgtaccgt
     8161 caaagatagt ttttgcactt atcccacgga aacgaataga gccaggatta tattctggta
     8221 attctgatgg gtccaatgga tggttcctca ttcctgggat cacgatgata tctttatctc
     8281 cttggaaacg agtgttgaaa gtccaaacta catcgttcat gtcgaaaata tcaacatctt
     8341 catcgactaa aatgactgtc ttcaattctt tgaaggctga taaagcaaga atagctgctt
     8401 gacgctgaat accttcatcc gcttcgtttt ctttacggat ttgcatgatg gtcatcaatt
     8461 taccaccacc tgcaggagga ttgtagacat ttactacttt tcctggaata gctttatcga
     8521 ccaaggcaag aatacttgct tctgtaggaa taccggccat agatacgtgt tcttcactgg
     8581 gaccaatcgt tgtttgcatg attggattgt cttttcggtg agtaactgct ttgactttca
     8641 ctacttgtac agctggatta gcatccccag tatagccagg aaattctggc attgcttttc
     8701 ctgagttcgt gttgatatct tcttgcattg tttcatttgg catgatttct gcttcaatga
     8761 tgaattcagc acgagcaata cccacttcat cgactgctat accatcaacg atttgaatag
     8821 gttcgttacg caaagctcct gcacaccata gttcattgta gccaagtgga gtggtaggtg
     8881 gttcgaaagt cgtgccaatg gcaattgctg gatctaaacc aatattgata gtgataggca
     8941 ttggtttatt caaggcttcg aattgttttt ggaagtgacc aatgtggcgt ccgccaggca
     9001 tgatgtacat accgatcgta tctttatctt ctagaaccat acgatggatc gttacatcgc
     9061 tcattgtttt ttctggatct gatcctaaga ctacacccat tgtgatgtat ggaccagcat
     9121 cttcaggagt atttgtaggt gcaggcaaaa tgttgcgaat atcgaagtct gcatcggtag
     9181 ctagatgaac gacttcttgt gctggagcgt ctgctttgtc tactttcaca ggtttgattg
     9241 gattttctac agcgtctttc aagaaacgac caagtgttcg ataatcatgg tgcaaaattt
     9301 cacctacacg tttacgacta gccattgttc cgatgttcac acgtgtgccg gggaaacctt
     9361 tgatattgtt gaaagtcata gctggtcctt cttgagtcgg acgttttact gtaccgccag
     9421 caccgatata acggtaaaca ccagataatt ctgcttcagg atcgatttca atatctgttt
     9481 cgtgatattg acctggaagt gtttttagtt cttcgatgac ttttcttaaa tcatacggtt
     9541 gatctgtcat gagagattcc tccttagttg tctttaccta tctatagtaa aaggtgaaaa
     9601 agaggaaaac aattcaactt gttcggtaaa gtattctaca atggaatgga tgcgtttttt
     9661 tgataataga ataaactatg atatacttat cactaaaaga aagcgcatac ttagtgttgg
     9721 aggaaggaaa gtgaaaaaaa tggaggagtc atttcctaaa gcagtaaaag tggaaaatat
     9781 agcgaatatt ttgaaagtta catttgaaaa cggagaagta aaatacgtga aatctcattg
     9841 gacagaagag ataaccgatg ccttgcaatt tggaaaaaaa ggaagaggaa aaagaaaaaa
     9901 tcttctcgca ctttctcgta atatgtggat tgggactgaa gtaacgattg aagcagatgg
     9961 aacagtattc ataaatggaa aagacaggta tacgccagaa gagttgtggt acaaaggaaa
    10021 gaaaagtata ccagaattat aaaaaataat taaaaaaaac agcgcgaaac aaggaacata
    10081 ttacttcctc gtttcttgct gttttctact atttcagcgc tcttatgact caaatctaaa
    10141 tgtggttgtc acaaccttat tctgcacgca aagcgatagc ggcgtctttt ttcgctgcca
    10201 ttcgtgcagg aagatgaccg ccaagcatcg tcaatatagt agagacgatc actagaataa
    10261 tcgcatggac aggatttagt tgagcaacat tttttagatc cgtcatgttg tataagatgc
    10321 tattgatagg gaacgtagct agccatgcaa taaacacacc taatatccca gaagaaatac
    10381 ctaaaataca cgtttctgcg tcaaatacgc gagtgatgtc ttttttacgt gcacctaaag
    10441 cttttagtac accgatttct tttgtccgtt caatcacaga tgtataagta atgatactga
    10501 tcatgatcat acttgttact aaagagatcc ctgcaaaagc aataaggaca taagtgatag
    10561 catccatcaa tccgccagtc agttcagtca tcgttccagc taaatcagta taaatgattt
    10621 tatcttcatc tgatttgcct ttattgtaat catctagata atccaagatc ttttctttgt
    10681 cttcaaaatt gttcggataa atcatgatac ttgaaggcaa gctgtctcca cctaaataac
    10741 taagcaatgt ttgtttggca ttttcgtcaa ctttttcagt agttaagaca tttacaccac
    10801 tgtctctttg agctttgacg atttctgatt ctttgttttc gttgacgatt tgagtcgtta
    10861 attgatcgct ataagcgatt cctggagata agagattcat tgtagaagaa gatttcacgc
    10921 gtaggatccc cgagatcttc aattcatcag aagcattctg atacatcgca tcataatctg
    10981 tattagggat aaaattccca gttggaagtt ttgtatagta agtattgttg tttactaatt
    11041 tgaatgttgt accaacgata tcatcaaaat caagtttttc gtcttctttc acatcaaatc
    11101 caagattctt caatgcgttg atattagtat tattattgcc gtctacgatc agcacgacat
    11161 ctgttgctga ggctggataa cttccggcaa gtaaagaata attgtctttt aaaaagtttt
    11221 ctttgctagt gtcaagctgt gtagggaaac ttgacactcc cacacctgtc attgctgaca
    11281 tcgtactaga taaagaaaga gattccgtat caggattttg attcgagaag ctgacaggct
    11341 gtactttgcc atttacatca cgtaaaagat tgattcctgt agtacgggta aatccgatat
    11401 tattacttaa gttaggatcg atgtctgtta cgtaatcgac gtatgtttga tcaatcttat
    11461 tcgtgtgctg tgctttatct tcttcgctga cttttgcagt aatcgttttg ctgtctggat
    11521 aatccgcttt gcttgcaccc aatccggcat cgtcacgagt ctggttagtc gttaccttag
    11581 aaatagtaat cggaaatttc gccatcgttt cagcttgcgt gttatcgatt tgtttttgga
    11641 atccagaaga aagggacaga acgatcgcga ttccgatgat cccaatactg gaagcaaagg
    11701 aggtcaagaa agtacgtcct ttttttgttc ggatattgtt gaaggaaagc tttaacgctg
    11761 tccaaaaact catctttgtt ctgcgtaagt taaattgatc atcttttggt cgttcgatgt
    11821 gaggatggga atctgttaag atcttaccat cagaaaattc aatgattcga tctgcgtatt
    11881 gatgggccag ttcaggatta tgtgtgacca tgatcactaa tttttctttc gataactctt
    11941 ggatcaattc catgatctgg atacttgttt ctgtatctaa cgctccggtt ggttcatcgc
    12001 acaaaaggat atctgggtca ttggctagtg ctcgagcgat ggccactcgc tgcatttgtc
    12061 caccagataa ctgatttggc tttttatgca tatgatttgt caagccgact cggtgcaatg
    12121 catcttctgc ccgttttcgt ttttcgtctt tagaaacacc acttaatgtc atcccaagtt
    12181 caacgttttc aataatgcct agatgaccaa tcaagttgta actttgaaaa atgaaaccga
    12241 tagagttgtt tcggtaggca tcccagtcac tgtccttaaa gtctttagta gactttccgt
    12301 tgattaccat atcaccggaa tcataattat ctagtccacc aatcacattc agcatagtcg
    12361 ttttaccaga accactagga cctaaaatcg ctacgaattc tttttgtctg aaagcgacag
    12421 acacaccatc caatgctttg gtggtggttt cgccgacttt ataatatttt tttatatctt
    12481 ttaattgaag cataatcacc cttctcccgt attgaaatat tcggtatact aataagtata
    12541 tcagaacaat gatcgtagta tacgaaataa atgttaactg aagatgaact acaagaaaaa
    12601 atctagaaat ttttcaaaaa acagagggag atcaatgaat cgtattttag tagtagaaga
    12661 tgatccgcaa ttacagttgc tttaccgttc tgtgttggaa cgagtgggct tttcagtcat
    12721 caccgcattg aatggagagg atgcgctgaa aaaactagaa gcgacacgta tagaattaat
    12781 cattacagat atcatgatgc cgaaaatgga tggctatgac ttgctagact cattacgtag
    12841 caccagattt gacacacctg ttctaatgat tacggcaaag gccgattttg aggataagaa
    12901 acgcgggttc aaattaggag cagatgatta tatgacaaaa cccatcgatg tcaatgagat
    12961 gatcctacgt gtggaagcat tgcttcgacg ttcaaagatc aattacagca gtagcttgat
    13021 aatcggacaa acaaaattag agctagatgc ttatacagtg ttacaagatc agcattcgtt
    13081 gattctgcct caaaaagaat ttctgcttct gtataaatta ctgtcttatc ccaacaagat
    13141 cttcactcgc caacagttga tggatgatat ttggggatta gatacgaata cagaagaacg
    13201 aacgatcgat gtacatatca aacgtttaag gacccgtttt gaaaagacgg aagattttca
    13261 aatcatcaca gtacggggac taggctataa ggcggttatt taaagatgaa aaaaaagcta
    13321 ttttcacaat tatggatcta ttttgctagt attgtcttca tttcgatctt agtgacactg
    13381 ctgtgttttc tcggttttat tttcttattg tctcgacatt ctatctcgcc tgctgaatcg
    13441 gctggaaaac caacgttata cccgttattc gtctttgcgg ggttcagtat ggtcgtgggt
    13501 acaggaatat cgatttttgt tgggcggcgt atcctgcatc caatcagtgc gctagggaca
    13561 aatatgagtc tggtagcaac gggagatttt tcgattcgaa tggatgaaca gcagaaagta
    13621 gcagaagtac agcaactgta taaagatttc aacgttatgg tccaagaact caatagtatc
    13681 gagacactga ggaatgactt tgtgtccagt gtatcccatg aattcaaaac accactagca
    13741 acaatccaag gatatgttca attgttacaa gccccaaatc tctctgacga agaacgccaa
    13801 atatttttat atcggattat cgaaagtatc acgcagcttt cacaattaac agagaacatc
    13861 ttaaaattga acaaactgga aaaccagcgt atccaactgg aaaagaaaga gtatcgatta
    13921 gatgaacaaa tcagagaagt aatcgttttt tttcagccta aatgggagaa agaacagcta
    13981 gaattggata tcgaacttgc tgcagtaaat tacacaggca atgaagagtt tttgtaccaa
    14041 gtgtggctga atatcatgga taacgccata aagtacaatc aaatcaatgg acagattcat
    14101 atcaaactat ttgagacagc cacagagatt gtactggaag tgaccgattc aggtgtagga
    14161 atgaatgaag agaccagaga tcgaatgttt gaaaaatttt atcaaggaga tacaagtcgt
    14221 caaatttctg ggaatgggct aggactttca ctggttaaaa aaattctaga actgcatgat
    14281 ggacgtattg attatagcag tatcgaagga gtaggaacga cagcaatgat acggttaagt
    14341 aaacaatagg aaaaagaata aaacccctaa aaattctatt gaaggttttt aggggttttt
    14401 agtcgttttt ttgtgattag ggaaatgttc ttacggttct tttggcataa aaatcgctgc
    14461 tttcttattg aactacgaga aatgttagag gtgtcattga aacaaggaca tgatgcaaga
    14521 aagagagcga aagtcatgcg tcgctctctt tttttgggtg gatagatgga cagaaaatgt
    14581 agggcttagt tttcttcttg atttaaatac tcccaaaatg tatctgaagc caaagaatgc
    14641 tcaccgactt tacgagtaaa tgcaagataa ctctctgttg ttggagaaat agggagaatc
    14701 acagtatttt cttttaatag attcttgact gttttttcca taacaatagc aatccccatg
    14761 tcatttgcaa tcatattgat gatcaagtcg attcttgctc ctttataaga gatttttggt
    14821 tcaaatccag cttcgcgaca cagatctagt acaggttgat acaagttcgt agtggggcca
    14881 aggagcaaaa agttctcctt tttcagttcg cttagatcca atttcttttt actagctaaa
    14941 ggatgatttt taggtagaac tgctacaaaa tcgtctgttt cagtaggcaa ccactcaatc
    15001 gaacttggtg gggcctggaa acttctagca aagtggatcg tgtcactatg ttcattggcg
    15061 tcattgtaag ttaattcgac actttccact tctttcaact ggactgtaaa ttcaggatgg
    15121 cgtttcaaaa actcggtgat tttggaaaaa cctttataat tgatcattgt cggtatcgtt
    15181 aagatattta atgaaagatt gttgtctttc gtataggttt caagcacttc ttgcatagca
    15241 tggtattgtt ctacgacatt ttttacatat gggagcaata agcttccagc ttcggtcaat
    15301 tcgatttttc tatgggcgcg ttcgaataat ttgacgtcta attctgtttc taacgaaaga
    15361 atctgtttag aaatatttcc ttgggtggtg aattgtcgat cagcagtttt ggtgaaattc
    15421 attgtttcgg ctaaatcaag aaaaacttcg aatttatgga tatccatcat attcctccta
    15481 cttattccaa taaagaatac tttaacacaa aaaatgaatt ggtgtaatct tctctgtctg
    15541 ttacaatctt tcttgcagcg aaagcaataa ttacagaaat ttttctgatt tttttcgctt
    15601 cgtagaaaaa gagaggagtt ttttgatgcc aactggagtt ttgatcaatg taggttccgt
    15661 tttgtttggc ggactaattg gcggattagt aggaaataaa ttaagtgaac attttaaagc
    15721 acagctgacg atggtttttg gtgtgtgttc gatggggatg ggaatctatt ccattgcgcc
    15781 gatgaaaaat atgccggctg ttatttttgc tttagtgata ggaacagcga ttggtttgat
    15841 tgttcattta ggtaatggga taaataaggg ggctgcgctg atgcaagtac ctatttctaa
    15901 aatcttccct agtgaaaagc ttggtatgac ccacgatgaa tttatcagca cattagtgac
    15961 ggtcattgtt ctattttgtg caagtggtac ggggatctat ggaagtttag attctgggat
    16021 gacaggagac agtacgattt tgatttctaa atctgtcctt gattttttca cagcagctat
    16081 ttttgcgtgt aatttagggt atgtcgtttc tgtgatagca attccccaat tcattatttt
    16141 ttatattttg ttcttattgg caaaatttat ttatccattg actacaccag acatgatctt
    16201 agattttaaa gcttgcggcg gattcttgat ggtcgctacc ggttttagaa tgatcaacgt
    16261 gaaaatgttc ccagtagctg acatgatacc tgcaatgatc gttatcatgc cactgagctg
    16321 gatgtggaca aattggatta tgccactgtt ataataaaaa tcaaagagaa gccatttttt
    16381 gctgactaaa aaggatcaaa tcttttagtc agtttttttg tatttgacaa actgattttg
    16441 aaactatatt atatgttgta taatatagat tgaaataaaa tattacactt gagaatagaa
    16501 ggtgacgtaa tgcacatcca gattccaact gttcttcttg atggtactgt gctagcagca
    16561 ttaagcaagg aagatatgta tgggtatgcc ttaacaaaaa tcgtacaaga aagtctctct
    16621 gtcagtgaat ctacactata tccggttttg agaagattga aaaaagaagg atatcttgag
    16681 acctatgatg aaccttttga aggcagaaac cgtcgatatt actcgttgac accaatggga
    16741 agtcgtcatt tagaagaaat tattgatgaa tggattgttt ttcgagattc tttagaccgg
    16801 ttattggagg agagcacaaa tgaatgaata tttgaatgaa gttgtggatc atcttcaaga
    16861 aattcctgaa gacgatcgtt ttgatttgat ccagtattat gaggaatatt ttttagattc
    16921 tggaaaaaca ttagatcaga tcattgatga atatggtaca ccaaagcagt ttgccttaaa
    16981 attaaaaatc agttactttt cagaagaaga acaagcagaa aacgagttag tgaaaaatcc
    17041 aaaaagacaa attcgcctga tttggttaat cattatcggt ttatttgctt ctcctgtcct
    17101 cattccacta gcactggggt ttatattggt tattggggga ctattggttg ctttactagc
    17161 gctcattttt agtatctatg ttttatgtat cagtatctta ggtgttggcc tattcatgat
    17221 catctcagga atcagtgtgt ttggacaatc gattctgagt ggattattct ttatcggtgt
    17281 cggtctgttc gccacaggaa gcgcagtatt tatttcgcca ctgttgctga aaggaacaaa
    17341 agggcttttt tatgtcatga tgaactttgt taaatggatg ggacgacgtt ttattacaaa
    17401 aagagatctg cattcagcgg gcgtttgaag gaggacacat aaattgagat tgaaacattt
    17461 tttgattacc ggtattggtc tgatgcttac tggtggcctc ctagcaggtg caacttatac
    17521 attaggcgct caaaagagtc tgacttggga taatggtcct cggttgatcg atatggttac
    17581 tgagacaaaa aaaatcaagg atgcaaacaa aatcgtcatc catgcagaaa atcaaacagt
    17641 ttcggtcata cgcggattgg attttgagat aaaaacgact tatgataaga cagaaaagcc
    17701 agaaataaag gagaaaaatg gcgtgttgac tgtgaatgca gtcaaaaaat caacaggtgc
    17761 aatcattagt ttagaagaca caagtgcaca aatcactatt gttgtccctg cagatcatga
    17821 attatctgaa ttgacaatcg aagggaataa tagtgcaatt gatatcaacg aagtatcaat
    17881 caaaaaagtg aatactaaga cacatgatgg ctgggtatcg ttcaatgaat ttacaggagg
    17941 aacaataact gcgaaagatg aattaggctc acttcgatta gatgaggcca atgtggataa
    18001 cttgatcgtc acaggtgaaa acagcaactt ccatctagaa gacagtttga tcagcaaaga
    18061 aggaaaagtc atcctaaacg gtggcaattt atctttgcat gagatggaaa acagtggcta
    18121 tactatccaa acgtctggtg aatcttatat tgaaaaagac taccagccaa taacaaatac
    18181 tttgttagaa caaggacaga aaaagctaaa gatcactgct aaagacacat cgatcaatat
    18241 tacgacggat caagaggaca atgaaacaga ttgatcctgt actcaactgg atattttaag
    18301 aaaaaacagc aaagatcctt ccaatctttg ctgtttttca tcgcttaacg cacataacta
    18361 tagtaaaata agaaagaaaa tatctaaaaa gaaaacaggg tgaaagagta tatgaaggta
    18421 gaagaaggtc aaagagaaaa gttaaaaaca gaaatggaaa gattacatac ttatatcact
    18481 caactaagcc aaaccttcta tgatccagac aaagaaaaag tgatggttaa ttacccgaac
    18541 aattcagaag gtcgccaatt agagcaagtg taccatgaag ttttcaaaca tttattgacg
    18601 gtgaagaaag agctggatta ttattcttta ccgattatcg atacaggaat attgaagtat
    18661 gatgggaaga aggaacgatt tatctttaaa tctgttcggg aagatttgcc tctttcagca
    18721 gggatggatc tagaggtctt ggtagaagac tattttacag aagaaaagca ctgggttcga
    18781 acaaaattgg attatttacc gcaggcagcc ggagggacac aggcaagcgg ttggtacatc
    18841 actgaagaca aagagttaga actagaaggt gtaatggcgc gtattcgaaa aaaaaattaa
    18901 aaaagtaaca ttagatattt attgaaggat aaaggagcgc aggaagtagc atgagagaaa
    18961 aaagagcatt ttatatcaat ggttatgcag ggattgtcgg attggtcgta ttagctttag
    19021 ttggattgtt tttcttctat ttaggtatgt ggcaagcgaa agtttgggca ctttttttaa
    19081 gtatttttct ttggttgatc acgttattgt tgttaagttc ggcaactgta gtcagtccta
    19141 atcaagcaaa agtaattttg ttttttggac aatatttagg tacgattcga gaaaatggct
    19201 ttttccttac gatacctttg gcacaaaaaa tgactgtttc gttgaaagtt cggaatttca
    19261 atagttctgt tttaaaagtc aatgatttgg atggaaaccc catcgaaatc tcagcagtag
    19321 tagtctttaa agtcgttgat acagcaaagg cattattcga tgtagcgtat tatcaagatt
    19381 ttgtagaaat ccaaagtgag acagccattc ggcatatcgc tagccaatac ccgtatgata
    19441 cattcaatga gaatgatttg acgcttcgag gaaacacgac ggcagtgtct gacgagttgc
    19501 aaaaagagct gcaagagcgt ttagcagtag caggtgtcga agtaatcgaa acacgcttga
    19561 accatttagc ctatgcaaca gaaatcgcta gtgccatgct ccaacgtcaa caagcaaaag
    19621 ccattttgtc tgctcgacaa accattgttg aaggagctgt cacaatcact caaatggcct
    19681 tagagcaaat cgaagatgga caagaaatca atttcaccga tgatcggaaa gtccaattga
    19741 tcaataattt acttgtctca atcattacag acaaaggaac gcaacctgtt atcaatacag
    19801 gcgacatgaa agaccgtaag tgacatactt tttgtaagat aggaagaagg agtttaggac
    19861 ggattattgt cttgaactct ttttttagtt gttttaaaga aaatgacaaa attgaaagaa
    19921 gaatgatagt tgattcgaag gcagaattgt tcgctttttt ctataatcag tatataaagt
    19981 aagagagaag gggaaaaagt gaaaaaactt ctgatcggtc tacttgtttt catcgttgtg
    20041 gtacttgcag gggtgaaaat cgcagattac gtagttatgg gtggagaaga atactatgta
    20101 caaattacga cagatggtac aaaaacagtt tcaaaatcag actctggaca agaaacagtc
    20161 aactatagat atatcctgcc tggctatacg aaagagggta aggaaaaaga actggacttc
    20221 aagggacaat tgactcgacc gcttcgtaaa ggagcatatt taaaaatcac ctggaacaaa
    20281 aataaaggtg tcacttcgta tgaagaagtc gaaaaaaaag acgtgccgaa aacagcactg
    20341 gaaaaactaa ttaaagggta gggaaagaaa tgaacaaaat agtcgaagta cagaatgtaa
    20401 caaaagtata cggaaaaaac aatgagaaaa agacgcaagc attaagcggc atcagcttcg
    20461 atgtagaaaa aggcgaattt attgggatca tgggcgcttc tggttctggt aagtcgacac
    20521 ttttgaatat tttatccaca ttagataaac caacagatgg gcatatccga atcaatcaaa
    20581 aagatgtgac cacattaaaa ggaaatcaac tagcagattt tcgtgcgaat gagattggct
    20641 ttattttcca agatttcaat ttattagaaa gcttgacagc acaagaaaat atcgctgttc
    20701 ctctttcttt acaaggcgtc agaccaaaag aaatcaagca acgtgtccaa aaaattgcag
    20761 aacgtttatc catctctcat attttggaaa gctatccttc tgagatttcc ggtggacaaa
    20821 aacaacgggt cgccgccgca cgtgcattga tcacacaacc aacgattcta ttaggggatg
    20881 agcctacagg tgcgctggat tccaaaagtg cccgggatct tttagatacg atggacgaat
    20941 tgaatacaaa agatcatgta tcgattcttc ttgtgacgca tgatccgttt tctgcaagtt
    21001 attgccaacg tattttattc atcaaggatg gcggtatcca tcaagaagtg aaacgtggag
    21061 atcaaagccg tgattccttc tatcgagaaa tactatctat tcttggaaac ttggaacagt
    21121 agggggagat tgactatgct ttggaaatta tcacttactg ggatcaagag ccgtttaaga
    21181 gactacttgg tgttattctc aggattagtt atggcatctg ctatttttta catgtttgaa
    21241 tcaatggcaa gcaacgaagc ttttttgaaa agtaatacga tcgttgcggc tacagttatc
    21301 attttccgat tcggttctgt tttactaggg atcatcacat tcgtttatat tttatatgca
    21361 aattcctttt tgatgacgat gcggcaaaaa gactatgcaa tgtttatgat gctgggagca
    21421 aaaggcagaa aaatcgcaca gatgattttt tctgaaacat tcttagtagg gatcgcagct
    21481 actttagtgg gttcagctgt cggtgtagga ttgacaagta tcgtccaccg attattagtg
    21541 gatcagttag atctcaatat ttcgcatttt acacctttta gtattcacgg gttgttgatc
    21601 actgttttgt tttttgctgt tttgttttta ttagctgctg ttgtcaatgc tttttcgatt
    21661 gtcaaaaaac cgattttagc attgattcgt gcggatcaga caccaacaag aatcaagcag
    21721 cataaattct tgtttttcct tgaagtagta ctgggaattg cttttctagg ctgcggttac
    21781 tatatgatga aacacgtggg gacctatcaa gtctttggta taggggtagc cttagttacg
    21841 atcgtattag gaacttattt cattttccat tctgtgatca ttttcttttt atcattacta
    21901 aagaaatcag aacagatttc attgaaaaaa ttgaacaatt tcacattgtc acagttaaac
    21961 tttcgtattc gtgaatatac acaaatgctc tcaatggtcg ctatgctgtt tgccttggca
    22021 ctgggcgctt tgacggtagg attaggtttt aaaaatgaaa ttgcagactt gacagaaaac
    22081 aattctggat atgatctagt gatgaataat cctcgggcag aagatcagca aaaaatcaaa
    22141 gaactttcac caacactttc tgcttcttat actcaaaaag aagatgcggg aacgatttac
    22201 tataatgcaa gtgaatttgc ggctcaacca ttgatcatga taaaacatga agatggaaca
    22261 ccgccgaaga tcacgaaaga aaaagttcct gctaaaaaaa tgaatgaact gactgtacaa
    22321 gaggaacttc gaagctatga attgccggaa cagcaagaaa aagaaatcaa ggtagtcagt
    22381 caatcagaat ttgatcaact gaacttgcca caatccactt tacagctagt tcaggtaaaa
    22441 gacttccaag caaacctgaa accaatcgaa acacttgttg ctcaaaataa aatgaacaat
    22501 ccatctttgc aaaaagtaga gtacagcaac cagaaatatg aaatgtatga agtgtataat
    22561 ggtctattct ctggttttga atttatggga ttcttcctag gtttagcttt cttgaccatg
    22621 cttgctagct gtttgatgtt caagatcttg tcaggaagca agagtgacat tgctcgttat
    22681 gaaatgcttg aaaagatagg aacacgacgc gggttgctta aacaatcgat ccgtagagaa
    22741 gtaggcgtgt tattcttagc accaggaatc ttaggtgtag tccatgtctt attcggatta
    22801 caaatgtttg gtctattcat gcaaaatcca tacaaagata tttgggttcc gtttacgata
    22861 ttcttcgtat tgtatttcat ctactatgta ctgacgacat ggttatatac tggaatcgta
    22921 ttgaaaaaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaagcaaaa
    22981 atagctcctc atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    23041 ccgtttgttc ggaaaaagag gttgtgacaa tatttttgtc acaacctttt tttcacggtc
    23101 ttttattaga atatagtaaa ttttttctcg ttactcacgg aataagctgt taagaaaacg
    23161 gtttccatgt atactggaat cagtgcaaat gaaagaaaag agggtgcaag gaacatggaa
    23221 atcaatgaac aaaattttgg cgaaaactat cacttgatta cgatcacgaa tcaaaatggg
    23281 acatctttgt ctgtcagtga tcttggggca cgggttgttc attttcaaac aataatcgat
    23341 aatgaaaaac gagagttgat cttaggtttt gatgaagcaa aagaatatct agaaaaagac
    23401 ccttatatcg gtgcttcaat cggacgtatg gctggacgga ttacgaatgg cacattcaaa
    23461 ttagatgatg aaacgttcga agtaaaaaca gacccggaca ctggtcattg tttacatggt
    23521 ggtgcaccta gctttgaagc caagcagtgg cattatacag tcatcaatgg tgaaaacgaa
    23581 gcatcggtca ttttctatca gactagccca gatatggaaa acggatttcc tggaacttta
    23641 gatgtagaag tacggtatac gctgacgaac gatgatacat ggcgagtgac ggtccaaggg
    23701 atcagtgacc gcaaaacagt attcaatcct acaaatcatg tctatttcaa tttaaccggt
    23761 gatccagcac agtcaatcga cgaacatata ctttggttga atagccgatt atttgctacg
    23821 ctcaataaag atacaacgcc tacaggagaa gtagtttcag taagtggcac agcatttgac
    23881 ttccaacgtc ctaaaaaatt aggtgaggtc tttgccagtg attttcgcca atcaatgata
    23941 gtgggcggca tcgatcatcc atttttgttg aaaaaacaat ctggtttact aaatgctgcg
    24001 gaattgacaa gcccagatga aaaagtatcg atccaagtaa aaacagatgc ttcaggaatc
    24061 gtgattttta cagcaaattt tggagaagat ggaccggaaa tgcgcgggaa taaattagta
    24121 aatcatggcg gtatcacatt tgagactcaa gaactgccag gagcggagcg acttgaaagt
    24181 tttggagatg tcactctgga acctaaccaa gtacgcgagt ttgtaacgga atacaaaata
    24241 aatataaaga aatagaataa attaaaaagg gagtgtgaca gtgtcacatc ccctttttaa
    24301 tttattctat ttctttaaag gcagatcaag ccagaatgat ttgtctaaac ttaacaaggt
    24361 tagtacagga acagttgcag ctttaactgt acgagtagtt gtttctggta tctgtcttgc
    24421 cgtaaaattg ttccacaaat cccagattga agcgacacgg aagatggaag aagcgttgtt
    24481 gacgcgtttt atcgttgaaa attctaatcc tgtgattggc acgccatctg ttgtccattt
    24541 gattggcaca tcgaatcgct tagacatatt gaggatttca gcgtttgctt cttcagttgt
    24601 tgctgcttct tttttcggtt catcatattt tgtcaggtcg tacgcttcga tgatcgcgtt
    24661 caatttttgt gaatattggc gatctgtagc ataacgtcct tccaaatatt tggttgcttc
    24721 tttgtagtct tttgtttccg acttccaagt tggtttgtag aaatcaggat tgccagtcag
    24781 tccatttttg atcaagtcgg catagtcttc caaactttcc ttgtaagaag gatatttacg
    24841 gaattgtgcg cggatttggt agctttgtcc ggaactatct tgttcaagtg tattgaaggt
    24901 cacgctgctt cctttatatg atcctttgat accaaaaagg ttatgatttg gttcgcttga
    24961 taataagctg tttccagatc cactttcaag gatagcttga gccatcatga ctgatgcgta
    25021 gattccttct ttttgcccga tttcctgtgc atcatctgca atctttttca agaactgatc
    25081 agttgtttgg ttttgactga aatggaacga gccatctaat ccatctaaaa tagatgagcc
    25141 gcctgtcagc gaacctgaag cagcaggtaa acctgctaag tttactcgga cagcaaaatc
    25201 aggcgtgaaa tctgaaatag aagttactcg aactttatct cctggttgag gcgcatggat
    25261 aaattgtccg ttcccaatat agatacctac atggtgagta acaccggaag aaggtcccca
    25321 gaagacgagg tcgccaggtt gggcctgact tactggaatc tgcgtacctg catattgctg
    25381 ttcgcctgtc cagccaccga tataatgacc ggttgctttc atatatacgt aataagtcaa
    25441 accagaacag tcaaaagtat ttggaccttt tgctccccat acatatggtt ttccaagatg
    25501 cttgaatgct tctcgaacga ttgcttgttg gacattcgtt gtatttgtag gcaatacagc
    25561 atcaattggt gattttacaa caaaatcaga agaagcagaa gatggattct ctataccgct
    25621 ttgatttgct gtatttggac gagatgaagc attgttcgtt ccgccattcg atgaaccatt
    25681 gtttggatgg tttggttctc tctgagtatt tccattgtta ttcgatccag atgggtttgt
    25741 ttgattcgaa gaattcccgc tgttcgaatt attgttatta ttgtttgaat tatttgaacc
    25801 attattattg ttgttattac ccacatctgg tttgtttggc gtttccggat ctggtgtact
    25861 agggacttct tcactaggat cagttggttt ctctgttgaa gtatccggtc cagctttaaa
    25921 ttctgcagta atagataaat tgttcgtacc tactgtaaag gtctttgcat cataatttac
    25981 ttctttggca ccattaacag cgactgaagc aatttgttca ccttcagctg gcgtaatagt
    26041 gtaagttacc ttcgtttccg ctaataaacc ttctaccgtt ccactttccg taatatccaa
    26101 aggttgctgt tcaggggaat ccgctgtcga agcagtcagt tggatcttac caatcaaaga
    26161 aggagcaatt gtaagggaat atgtctcggc tggcttttca ttttctgatg gatcagccgg
    26221 atcaatttca gttggctcgg tcgatcctgt tgttgagtta gttgttccag ttgggtcttc
    26281 tgtaaaagaa tccgttgttt cactagtagc tgagctggat gtagtcgtat ctgttgtgac
    26341 tgtttgatca gctatttgat tatctacggc aaatacggta ttggtcgaca aaacagacgg
    26401 agcaatcagc caagtagcag ctagcaaacg tatcgttttt ttatttttca taataaacta
    26461 gtccttttcc tttcattgat tttctgtctt attcagcatt cattagacat tttttggaac
    26521 agataatcgt gtcgaaaagc tttttctttt cataaatctg tctgtgtatt ctaaataaaa
    26581 caatttgtaa ataagcaacg agcattcatt cgtttttctt tgacgttcat tttattgcat
    26641 agaccacctt tgtttgggga taaacaagtg gggataaaaa tggttatgca tatgtaaatt
    26701 ataccaaagt ttggaaagta tcacaaaaat ttataaaaat aatgatccta aaaagaaaaa
    26761 gactgaaata tgtgataaac atctcatact tcagtctttt tcattaacta ttcataagac
    26821 attaggtctt catcaaaccc gctaacgaga gtagtgatca ttgctacgac tagactaaca
    26881 gccagtcctg ctaagtacca gaaaaaagta ccggaaatga atgcaggcaa tgtggtcaga
    26941 ctaccaaata caaaagcgat cgctttcact tgggcaaatc ccattacagc tccaccagca
    27001 gctgcaccaa tgcaagcagc aacaaatggt tttttcaatg ggatcgcaat tccgtaaaca
    27061 gttggttcgg tcactcccgc aagtaaaatc gaagcaaaag cagaaccgga gatcgaacgg
    27121 gtttttgaac gtttacttcg cagaaataca cctaaagctg caccagccat tccaaaattg
    27181 gctgctcctg tcaacggaac gattcggtcg aacccataag cagagatatt actgatcatg
    27241 acagggttta cagcccattg gataccaaat attaccaaaa tattccaacc accagcgatg
    27301 attgcgccac tgatcggtcc gttaatatcc atcatccaag taattgcggc agctaaacct
    27361 tcgccaaggt aaacacccgc aggcccgaat accattaacg tcaaaggaac cgtgataact
    27421 aatgaaagta aaggtacaaa tactaattgc atattttcgc gaatatattt tttgaggaat
    27481 ttttcaacaa aagaataaaa gaaaatacct aaaatcgccg gcatgacagt agacgtatac
    27541 tgcatcagta ctacaggaat ccccagaaaa tcagtagctg ctttgccact gtctgaaagt
    27601 aaagaggtca gattcggttc aatcagtgct gcagcaatga cagccgcaat atatccattc
    27661 actttaaacg ttttggcagc agaaaaagca agaaagattg gcagaaaata aaagacggca
    27721 ttcgacgcag cagacaaaat atgataagtt ccgcttgttt cactaagcca gccaatcgta
    27781 gtcgctaaaa caaccagacc ttttaatata ccagaaccgg caaacagcgg aatgatcggc
    27841 gtaaagattt ccgatatcaa accaaatagt ttttcgatcg gatttagctt ttctttcgga
    27901 gctgaatgat cgatttgacg cgtcaattcc ttgtaagcag gagccaatcc ctgatccatg
    27961 attatttctg catatctatc gagtaaaatg aaatacttaa tggaagggat ttgctgtttt
    28021 aatttttctg cattggctac tggctgtttt ttcaatttga tacgaagccg agtcgcacat
    28081 ggaaggacag aatcaatatt gtcttttcct cccaactgtt caatcagcat atgaatctgc
    28141 tcggacagta ccatctgttc aggattcatt gtcgtttcca ttagaagacc actccactca
    28201 ctaatataat attggcgtaa ggtgtcattt cgcctgtacg aataaatgca tgacagttat
    28261 tcaactcttt tttcatttct tcatgtggga taaagctaat cggtgtatct ggtaacaata
    28321 atttgatttt ttccaatatt tccgggttca ttgtctttat ttcttccgcc aattcaatcg
    28381 attgtacctc aagttcttct aaaacgtttt ctaaaacaga taaaaaactt ggaagaccgt
    28441 ttgagacagc caaatctatt ttttcagtac caaaggggac aggcatgcct gcatccccga
    28501 tacttaattt atcaaagtgc cccatttgtg cgattactcg tgaaatatct gaattgatta
    28561 cttttccttt tttcatgttt gtttcttcct ctcattgtta attttagact gctgattgtt
    28621 ctaaagcttc gcggtaaggg atagaaggtt gcgcaccata agactgcaca gcaacagaag
    28681 aggctagatt tccataaagg atcgcttctt tcaagttact aaggtctggc tgaagtgtgc
    28741 tgcttaatgc gccaataaat gtatcacctg cggctgttgt gtcaacggca tccactttaa
    28801 aagcagggac gataccgtgt tctttttctg tatgatagaa agcgccggca ctgcctagtg
    28861 taatgattac tgttccaata cctaattcat ggagtttttc ggctgctgcg accaaactgt
    28921 tatgatctgt cactctgatt cctgtaatga tttctgtttc tgtttcattt ggtataatga
    28981 tatctgtctt ttcaagtagt tcagtaggaa tcgtttcacg agctggtgca ggattcagga
    29041 tcgttgtttt tcctgcgtct tttgcaatag aaaaagctag gatcgtcgca tctaagctac
    29101 tttcaaactg agcaataata aagtcactgt ttgtaatcag ttgcttgttt tttaggactt
    29161 cttgttcatg gaaagcatta tttgcccctg catggatcaa gatactattt tctcctgctg
    29221 cgtctaccat aatcattgct tgtccggttg tggcgccttg gatttcagct atcccagatg
    29281 tatcgatgtt ttcttttgtc agtagatcta ataactgttg tccttcgctg tcagccccaa
    29341 cgcctccaat aaaagatgtc cgtgcgcctg aacgtttggc tgccactgct tggttggcac
    29401 ctttacctcc gccagcgtga aaaatctcat gcgcatgcat cgtttcgcct ggcttgggca
    29461 tgttggttaa acgaagggta gtgtccatat taatacttcc aattactgta accgtgttca
    29521 ttttacaaac ctccaactat ctcatttatt ttgatgggaa aattattttt taaaacgttt
    29581 tagtaaaacg ttttaatagt tcgataaaaa tatagcacac cttgaaagct atttcaaggg
    29641 tgcagttgaa aatctttttt ctaatcgtac aggtaatctt ttttcttccc attctttttc
    29701 tgggaattgg atccgatcta acaataactt ggcagatgtt tgtcccaatt cgaagattgg
    29761 ttgtgcgatc gttgtcagtt tcggcttgat atactcgcac atatctatat tatcatagcc
    29821 aataatgctg tagtcttcag ggatgctttt tccagcttcc tctaatccgc gatataatcc
    29881 aaaagctaat tcatcattta aagcaaacac accagttgca gcagaatcaa gaagttctgc
    29941 tgtgatctgg tagccacctt gttttgagaa ctgtgtaggt aagaggatca gttggtcgtg
    30001 agggatttgg taaacatcaa gagcagattt aaacccctca atccgagcgt ggacattttc
    30061 cggtggattt tcaggataga caagggcgat cgtttgatgt cctagactaa gcaagtgcat
    30121 gcccgctagg taaccaccgc gaaaatcgtc tgttcgtacg gcatcactaa atccttcaga
    30181 ttttttctga tccaaaacaa tgaagggccg gccttgtttt ttcaaatttt cgttgattgc
    30241 atctgtagag acagcagaag tcgcgataat gaatccatcc acacctcgac gtgtgagctc
    30301 ggctagatat tcgatttctt tttgatgatc agaatcagca ttacataata tcgtgacgaa
    30361 attctgctta tacaagatat cctcaattcc tcgcatcagt gtactgaaga atggattcgt
    30421 gatatcagga acgagtacac caattgtttt cgtttccttg gtaatcattt gtctagcaaa
    30481 atagtcaggc tgatagccta attcttcttt ggcagctaaa actttctgaa cagttttcgg
    30541 actgaatttc tccgtatttc catttaatat caaggagaca gttgtgattg aaacgccgga
    30601 aagtttagcg acttctttga ttgtgatctt ttttttcacc atgttcatac tcctatctaa
    30661 atcctgtggt tattttccca tgaaatcaaa aagaagtaaa tgaaaatcac gtgtgccagt
    30721 acttatttgc tttgttggat agcttcgatt atttgtttcg ttgagcgtac tcgtgcagtt
    30781 ttcggaaata tcttttttat gaacaattga tgggattctt tgtcacgatc actgcaagca
    30841 tcttcagcaa gcacgatatg gtacccgtgt tgaaaggcat ctaatgcagt agcatataca
    30901 ccgtttgttg tcgctacacc gcttaaaatg atagtttcga ttccacgccg gcgaagttga
    30961 agatcaagag aagtaccaaa aaaagcactt ggattgtatt ttgtgatagt caacatatta
    31021 tctgtatctt tcaaagaatc cttcaaaagt aaatcagtga attctggagg tacagaagca
    31081 gtcattccac ccatatcagt ttgttgactc aaataaggga aagtggagat gtctacgttc
    31141 actaaaactt tcaaggcagc ggtacgtttc aatgttctta ctaacaaatc attgtttgtc
    31201 agaatagatg ctgcagtgtg tggagagagt gtaccgttat ttacgatctc tttttgcatg
    31261 tcgatcgcga caaaagcagt ttttgataaa tcaaaaatca tattttcctt taaatcattc
    31321 atgatgttcc ttctttcaaa taacaatgtt ttttatgtaa gtaaatcata aatcgtttat
    31381 ttgtcaacta aattaaagga ttcctttctt tagtctagag aaattcagtg gtaaaataaa
    31441 taagtgtgag aaaacgcatt cgatgcatag atatagcgag aaatatcgtg aaaatggagg
    31501 aatcagagtg aagtatgtcg aagtaaaaaa tgaatacaaa cgatacaaaa tgggagaaac
    31561 gaccattaca gcaaatgacg atatttcttt ttcaattgaa aaaggcgaat tagtgattat
    31621 acttggtcca agtggagcag gaaagtcaac gattcttaat attttaggtg ggatggatac
    31681 acctgatgaa ggtcagatca ttattgatga cacagatatt gcgcaatttt cagataaaca
    31741 gcttactgct taccgacgaa cagatgtagg gttcgttttc caattctaca atctcgttcc
    31801 taatctaaca gcaaaagaaa atgtggaatt ggcaacggaa gtttctcctg atgcgcttga
    31861 tcctgtagag gtattgaggc aagtgggcct agctcatcga ttgaataact ttccgtccca
    31921 attatcagga ggcgaacagc agagggtttc aattgctcgt gcactagcta aaaatcccaa
    31981 acttctttta tgtgatgaac cgacaggcgc gctggatttt gaaacgggca agcaagtatt
    32041 gaaattattg caaaacgcta gcagacaaca cggcaatacc gttttgatca ttacacataa
    32101 ttctgcttta gcgcccatcg ctgatagagt cattcacatt aatgatgcta aagtaaggtc
    32161 tgttgaatta aatgatcatc cttcatccat tgatgagatt gtatggtagg gggattttat
    32221 gaaaacaaga acatatctta aagcaggttt tcgcgaaatt cggcaatcta aaggacgatt
    32281 cattgcaatc atccttattg tcatgctagg aacactttta tttgtaggtg tcaaaactgc
    32341 tggacctgtc atgcaaaaaa cgatggatca ctatgttggt acagcaggac tttctgattt
    32401 gcaaatcgtt tctactggtg gcttgacaca aaaagatatt gctcaagcag aaaagatccc
    32461 agatgcaaaa gtagaaacag gaaaacagat ctattattcc aatactggca aaaacgaagt
    32521 gattcaaata ttttcttata acaaaaataa caagcagaac aagctacaat taactgatgg
    32581 aaaactcccg gaaaaaccaa accagcttgt attggatgaa aaagcggaag atgaagggta
    32641 taaaatcggt gatatttatc agattgattc agatgaattg aaagaaaaag agtataccat
    32701 cacgggcttt gtccgttctc cattgttcat caacaatttg gagagaggat acgcgaacgt
    32761 cggaaatggt agcgtcgatt attttgtcta tttacctgaa tccgttttta aaacagatat
    32821 ttattctgtc ttatatcttg attttacaaa cgtaaagaat ttgaatacgt acagcaaagc
    32881 ttacaaagat aaaatggagc aaaatcaaga aaaggcagaa aagtatttac aagatcgacc
    32941 cgaagaacgc ttagaagagc tgaaaaaatc ggccaacgaa gaattagaac cggcacagca
    33001 aaaaatagcg gatggcaaag cacaaacaac gcaagcaaga acacggttag aagatgctaa
    33061 aaaacagtta gcgcaacagt cagcaactat ccagcagctt cctgaagcgc aacgaacaat
    33121 cgctgttcaa acattatctc aacaagaagc tcaacttgct gaacaagaaa ggcaattagt
    33181 tgagaaagaa cgtgaactgg cctctgcaga ggaagaattg aatcgaaatg cagaaaaaat
    33241 tgaaaattta actaaaccat catacctata caatgaacgt tcagaaaatg tcggttttca
    33301 agaatttggt gatctgtctg atcgtatcgc tgcgattgcc aatgtttttc ctgttttctt
    33361 tttctttatt gccgcattga tcacctttac gacaatgaca cgtatggtag aagaaaaccg
    33421 cagggaaatc gggacattaa aggcgttagg ttatacgaaa gttgaaatcg caaaaaaata
    33481 tgcaatctat gcaagtctag cctcagggat aggtattgtt ttaggaacga tattaggaac
    33541 gaatcttttg ccaaggatca tttttgagct gtctaacgag cggtatgata ttgggagtgc
    33601 gcttattttt tatgactggc cgccgatttt acaagcagct gccgcattct tcattgccgc
    33661 atttggcgct gcaatgatcg ttttattcaa agatttaaga gaacggcctg ctgcattgct
    33721 tcaaccgaaa gcaccgaagc cagggaaacg catttttctt gaatacatca ctccgctgtg
    33781 gtcacgcctt agctttaatc aaaaaatcag ttaccgaaat ctcttccgat ataagtcaag
    33841 aatgcttatg gcgattatcg gaattgctgg atgtgccggg ctgatggttg caggcgtggg
    33901 attgaaagat tctctaagtt ctgtttcagc caaacaattt ggaccgatca ttgactatca
    33961 agcaatcgtt acattggatt catccacgga agatagtcgt caaaaagcag cggatatgct
    34021 gcagaagcaa gaaaaagtaa atgagatcct agctattcat actcaaaccg tagaattgag
    34081 aaaaaaagga catgcttcgc aaagtctgac agtgatcgta ccaagtaaaa cagctgattt
    34141 ggatcgtttt atccatttaa ctgatcttga agagaaccca cgctccttgc ctaaaacagg
    34201 aatcgctatt acgcaaaaag tagcagaact atttgactta tctacaggag atcatatttc
    34261 gctttatgat gaagatcaaa aagaatggaa agtcgaagta aaagaaatcg tgaaaaacta
    34321 tctaggtagc tttatttata tgtcccctga atattatgct tcagttactg atcaatcgat
    34381 gatggataat gcatttttgc tgcgtacaga taagatgaca acaaaagaag aagaaatgct
    34441 ttccgagaaa ctgcttgcca cagacgctgt gacaaatacg tctttcatgt ccaaacaaat
    34501 cgaaacgcaa gaagaatcaa tggcgaactt agactcggtt gtcttaatct ttgtcgtatt
    34561 atcaggactg ctggctttta tcgtcttgta taatctaact aatatcaata tttccgagcg
    34621 tgtacgcgaa ttatctacaa taaaagtcct tggatttttt gataaagaag tcacgatgta
    34681 cattgttcga gaaaatatca tctttactct tttaggaatc atcggaggat ttggtgtagg
    34741 atatatattg actgacttca tcttgcaaca agcgtctatg gagaatgtca tctttccatt
    34801 agtgatcaca tggtgggctt atgtcttatc agcaggatta actatcgtat ttacggttat
    34861 tgtgatgatt gttacccatt tcaaattaaa gcatatcgat atgattgatg cgttgaaatc
    34921 gaatgaataa aaacagaggt tgtgaaatca gcggtttgac ctgagtatta gagaaacaaa
    34981 cggcaaaata gtttttcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt
    35041 gaacaccatg aatcagaggt tgtaaaaaag ccgtcctgca tcgagcaaaa agaatactca
    35101 aataaaatta gctcctcatg ttttacattt gagaagactg attgccgtag ataaaagctt
    35161 ttgaacacca tttatagaag aaacaagaat attttgctat tttaaacaag aggatagaca
    35221 aatacatgtc tgatctcttg ttttttattg ggcaacagac ctcttagaat gtataaagat
    35281 aaattttaca aacgctttcg gttcagttat aataataaat gataacttgt atatataagt
    35341 tgtggtaggt aataaatcag ttgaatggag gtaaatatcc atgacattta aagacaaagt
    35401 catttaccaa atctatccca aatcttacaa agacagtaat ggtgatggaa ttggagattt
    35461 aaaaggaatc aaagaaaaac tcccgtatct gaatgatcta ggcatcgaca tgatttggct
    35521 aaaccctatt tatccaagcc ctcaaaaaga caacggttat gatatctctg attacacagc
    35581 catcgatcct atctttggta cgatggagga attcgaacag ctaatagccg aagcaaagaa
    35641 ataccggata gaaatcatgc tcgatatggt gctgaatcac gtttcgacag aacatgaatg
    35701 gttcaaaaaa gcactagcag gggaccagca ttatcaggac ttcttcattt taagagaaga
    35761 gccaacggat tgggtatcaa aatttggtgg aagtgcttgg tcgccatttg gagatacggg
    35821 gaaatattat ttgcacttat atgatcggac acaagctgac ttgaactggc ggaaccaagc
    35881 agtaagagag gaactattca aagttgttcg tttttggatg gaaaaagggg taaaaggatt
    35941 ccgtttcgat gttatcaatg ttatcggtaa agacgaacaa ttgaaaaata ataaagaaaa
    36001 tgatggcaaa cctgaatata cggacaaacc aatcacccat acgtatctaa aagaattgaa
    36061 ccaagcaacg tttgggacgg attccactat catcacagtc ggtgaaatga gctctacgac
    36121 gatcgaaaat tgtatcttgt atacagaacc tagtagagaa gaattatcaa tggtctttaa
    36181 tttccatcat ttgaaagtcg attatgaaaa tgggaataaa tggtctactc ctccatttga
    36241 ttttgcagaa ttaaagcgac tatttcatac atggggagaa aagatgagtg agggtaacgg
    36301 ctggaatgct ctatttttta ataatcatga tcagccacgt gcgctcaatc gatttgtagc
    36361 tatcgatcag tatcgaaaac aaggagcaga gatgctagca accatgatcc atttgaaccg
    36421 tgggacgccc tatatttata tgggagaaga aatcggcatg atcgacccag actattcatc
    36481 gatttccgac tatattgacg tagaaagttt gaatgcttat aagttactcc ttgaacaagg
    36541 attcacagaa aaagaagcgt ttcgtcggat caaagcaaaa tctcgcgata actctcgaac
    36601 acccatgcaa tggtcagaca cagaacaagc aggatttact acagggaaac cttggttaaa
    36661 agttggaggc aagctagaag agattaatgt agaaaaagaa ctatcttctg aagattctat
    36721 tttgtcctat tacaagaaat tgattcggct gagaaaaaca tatccaatcg ttgcacaagg
    36781 agattatcat gcttatgaag caaatcatcc ggaagtttat ggctttcttc gccaatttga
    36841 aggacagcag ctgcttgtat taacaaattt ctatgataaa gatacaagga tcgccatccc
    36901 agaaggattc acagaagcca atattcttat cgataattat tccgatactg ttcttcatca
    36961 aagtctggag ctaaaaccat accaggcaat tgctttgctg aaaaattaaa acaaaaacac
    37021 aaaacgtcgt tcctacgagc tattctcgta agatcgacgt tttgtgtttt atttaaaata
    37081 atcttcttca gaaaatgaag agttttcata ctctccttta tcgccataaa atttattgta
    37141 gcgttgtttg aatttacgga aaataaaagt aacgagtaat ttgactaacg tatagatagg
    37201 aataccaaaa ataacgccca caattcccat tagatcaccc atgacaagta aaacgattaa
    37261 gatcgtaatc ggatggatct gtaaacgatc acccattact cgaggaatca ccaagtcacc
    37321 gtggagaagt tgtacggcaa accagacaat gacaaatttg acagccataa aagtagagtc
    37381 ttggaatgca atgatcaaac ctggaaagaa ggcgataaac ggcccaatat atggaatgat
    37441 acataacaac ccagcagaaa tagctaaaac actagcaaat tctaatccga tcagtaagaa
    37501 acaaacccag tagactgctc caaggataaa tgaagcaatg atttgcccct ttagaaatga
    37561 acccaattgt tgattagcaa tctttgtcaa atttttaaaa tctgtacgaa atgctggtgg
    37621 gacaatcgct aaaactgact gataaaattt ttgaggatct ctcaaaagga aaaaagcgac
    37681 aattggacca gtaaataaag taataaaaat agctgtaaca gttgaaaaaa tgttgctgat
    37741 ccgttgtgcg ccactttccc aataatttcc aataaatcca gctatatcag acgtcacgtc
    37801 gtcaatagaa gcgaaaaatt gattaaatga atcggcaaaa ggtgttttct ccaaaaattg
    37861 tcggagactc tcttgaaaat cttgaaacaa atctggcaaa ctactgatca atgtctttgc
    37921 ctgttcttca agaaaaggaa taagccatat gcttccaagc cccaataaaa ataaaataat
    37981 cacataaacg ataatgactg acaagttcct cgaaaggtgc ttgctcaaac gtttgatgac
    38041 tggttccaaa agataataca agatcagtgc aaacaataat gggggcaaga tagtagcgat
    38101 aatgattccg acaggcttga atataaacga gatctgatgg aaaaaataaa tgatcagcgc
    38161 aagtaagata actattccta aaacatagta agaggttttt cctcctaaaa agcggatcaa
    38221 acgattcgtt gtatctgttt cttttttcac tctttttcct cctttatcta taaattcatt
    38281 atagacaaaa gcgggtataa aaggaaaatg atagaggttg attgcaaaaa tatcgtcaat
    38341 cattcatata aactttctca tcaattaaaa taagaaaacg cttgacagtt cttttaacca
    38401 tatgataaat taaatcctgt tgctaaaacg taactattac ggtttgcgaa aagcgaatcg
    38461 gaacattcaa atgaaagaag atgagaagag gagtgtcata aatgaaacga ttgataggta
    38521 tattggccat gctggtttta gcaggtatgt taactgcttg cggggcaagt gggaaagccg
    38581 aggacagtaa agaaaaattg agcgtgatga cgacctttta tcccatgtat gattttacaa
    38641 aagcaatcgt cggggatgaa ggagaagtag agttattgat tccagcaggg actgattccc
    38701 acgattatga accttctgct aaagacatgg caaagatcca agatacagat atctttgttt
    38761 ataatgatga aaacatggaa acatgggtcc ccgcaatcca aaaaacatta caagaaggca
    38821 atgtccatac gataaaagcg acagaaggta tgcttttact tccgggaagt gaagaaggac
    38881 atgatcatga ccatgaacac ggggaagaag gccatacaca tgagctagac ccacatgtat
    38941 ggctagctcc aagtttagca atcaagcagg tagcaaacat acgtgatcag ctgattgaag
    39001 cttacccaga aaaacaggaa gtatggacaa aaaatgcagc agcctatacc gagaaacttc
    39061 aagcactcca tcagctttat caagaaacct ttaagcaagc aaaacaaaga tcttttgtca
    39121 cgcaacatgc tgcattcaac tatctagcgt tagaatatgg attgaatcaa gtatcgattg
    39181 ctggtctttc ttctagtgaa gaaccgtctg cggcgagaat tgctgaactg aaacactttg
    39241 ttaaagaaca tggaatcaac tatatttatt ttgaagaaaa cgccaaagat agtattgcca
    39301 gaacgttagc aaacgaagca ggtgtatctt tagaagtatt gaatcctttg gaaggattga
    39361 cgaatgaaca aatagaaaat ggagaaaact atctttctat catggaagca aacctcgaag
    39421 cgttgaaaaa aacgacagag acagaaaacc cgctagaaga tactttgacc cccaaaaaag
    39481 aaaagagcgt ctacaacggt tattttgaag acgaggatgt acaagatcgc tctctttcag
    39541 attgggcagg aacttggcaa acagtagata cgtatgtaga agatgggact tttgatcctg
    39601 tttttgaata taaagaaaaa ttgaatcagg ataagacagc acaagaatat aaagaatatt
    39661 atacgaaggg ctatcaaaca gatattgctt caatcaagat cagtgagaaa gagatggcat
    39721 ttacgtttaa gaatggaaca acgaaacgtt caaattatcg ttatgtaggg aaagaaatct
    39781 tgacctacga ggcaggaaac cgtggcgtaa ggtatctatt cgaagcagaa gataaggaaa
    39841 gtggcgcttt tagatatatt caattcagtg atcacacgat tgcaccggct gactccgaac
    39901 attttcattt atatgcagga aatgaaagtc aagaagtatt gctgaaagaa atggagaatt
    39961 ggccaacatt ttatccagat acaatggatg gacgtgaaat tgcacaggaa atgcttttgc
    40021 actgataggc aaaaaagaac cgaagggttt ggatcggctg gtcatttagt cggtccaaac
    40081 tattcggttc tttttatgaa atcaatttct ttaatccacg gatagaggct gggatggtct
    40141 cgccgtttgc caacgtgact tctctggttt ttttattaat cgaccgcaca ttttgtgtat
    40201 tgataacata ggatttatga caacggataa aattccgatg cattttctcg atttctttga
    40261 tcttctcata gaattgtaat tgggcatgag tcaaatggac ttccagttta tgtggcgctc
    40321 ctagcgaagt agaaaaatat tggatgtctt tgatcggtaa aaatttgatc tcatcattaa
    40381 actctatttt gaagatatct ttttggattt gggacatttg aaaatcttcg aaaattttgc
    40441 tcaggatatg ctcgacattt tcttttaaat cataaagatc ttctttgacg atataatcag
    40501 tcggttcgat gttgcttttt aagattttca tcgacatttc cgtatgggaa gtcacataga
    40561 ttatttttcc taaaggatct aattcacgga tcaatttacc gatcgctaca ccatccattt
    40621 cactgtcttc tagttcgata tctaaaaagt aaataccaaa agaagaagtt ctgatcttgg
    40681 ctgcattgag aagttcttcc ggatcagagg tagaaatatg gacataactg tctaagtttt
    40741 caatcatgat tctatttttg atcaccgttg ataaaacttc taattgtttt ggatcgtctt
    40801 cacaaatata aatgggaatc atatcattac tcctttattt tatttgtata taaatcgttt
    40861 gagaaaaacg ctggttttca attgatgttt caaggaaaag ataaggattg gtctttgtca
    40921 attcatccaa gataaacaag ccaaggcctt cgttttttgg atttgttttt gttgagaacc
    40981 ctttttgttt caactggttt aacggtaatg tatttgttgt tgtattgatc acacgaatca
    41041 tcacggaatg attcttcttg agtatggtca attcgatttt tttcttcttt gcagcactag
    41101 ctccttcaat cgcattgtcc aagataatac caagcatacg tacaaggttt accgtatgtt
    41161 tcgtatccag ttcgattggc tcaggaaaat gaagctggac gatcaaccct ttttcctgtg
    41221 ccatcatgag ttttaatgac agtaaactac ggatctcagg aactttcata ttatccaaca
    41281 tacttagatt ggccggaaaa aggttcatca tctggcgtgt aggtaatatc gtttgatcat
    41341 aataagaccg taatccttct aagtcgcctt ctgaaatata gctgtgaaga gaaaaaagca
    41401 gattttggta atcatgtttg aattctctca agtcttgata gtttttttct agatctttga
    41461 tataatcctg ttgcatcctg acttgttgct ctaataactg atcagctaat ttcttattag
    41521 agattttcca ataataaacc aagatgatca ggccaacaca cagatataga atgaaaaaac
    41581 tcgtattcaa agctaagaca tcaggtgttt caccagaaaa acgggtataa aaaataatga
    41641 tatagtatgt aaataatatc aatgtcccga tcgtaccgat catacggcgg tccacagtgt
    41701 gaaaccaagt ctctaacgat ttttttaaac aaaaagctaa taaagcagat aaaaaagcgg
    41761 ataagacaat gtgaaaatac tgcaatgaat ctcctggttc aataaccgaa gatttcaaaa
    41821 caaaaaaatc gccgatgctt gttaagtgat ctcctaaaag cgacgtcaat aacccaaaag
    41881 gaagcgcgta aaccaattgt tgacgcgaag tcgaaaagaa gaaaaaagta agcatcagca
    41941 ttcctatagc aattagagca gtaaaaatcc ctaaaatcga atagacccat ccaatcaaaa
    42001 tacaagaaat cagaatgact cccgcaaaca gatatttgta tcggatgtga tggtcaatca
    42061 ataagataca aattgtgtaa atcaaatatt gcaacgctat ggaccataaa aacataaagt
    42121 acagctccca ttgattttta ccaaattata gcacaattgg cgtattattt ttttaatttt
    42181 tcagggattt tgaattcaga actcatacca aagcagcagc cagatacaaa aaagtcagat
    42241 aagttagtta attgtttaat gattgttttt ttcatgaaag attcctccaa aattcatcaa
    42301 gtagtttgta ataggaagca acgtagctat ttctaaaaca atcccaagta gcaaataggt
    42361 agaaaagtaa gaagctgaga aaaataacgt aatgagagaa aaaattagaa acatgctgat
    42421 cgccttcttt aataatgggg gacgtttctc gggttcaatg ggattgattg ctgtacctct
    42481 tgatccatag cgaagcagta acaaaaagca gctgattaac aaaaagacca tcattgggaa
    42541 ggagatagtc aattgcagat gtttcatcac caagggaacg ccccataaat aaagtagtgt
    42601 ccacagcaga caaacgaatt cagagtcgga atgcaatcca taagcatatc tgcggatgca
    42661 gatataagaa agaaaggtga ttcctgtttc cgcccatgag cctgtcacca gagaaaagac
    42721 tagaacgacc gctagttttc caagattatt caataaaact tctaagacaa attttacttg
    42781 gacatacacc atatcttctt ccgatttgtc tccgctgaat aaggcttcta ataatttgtt
    42841 cacaacttta tctttcatct catacgtacc tccaccacaa attttatgct aaaaaaaata
    42901 aaaaacgtct tttgtttcat aaaaagtggt tttgacatca aaaccgtcat gatctcctaa
    42961 aaaaatcttc gttttccaag aaccttacca aaaaaagtga cagttttgat acgaaaagta
    43021 ctttttatga caagtttgtg ctttttcgag tgatttatgt taaaaaatta ctgaccttat
    43081 aaaaggtgat ggagggattc ttatggaaaa tgaacaattg tttgaagtcg gcgaaacata
    43141 tgcatttaaa tctgatttat ttgaatcacc aataaaagga aaaatagaaa aaatctatga
    43201 aaatagtgtg atgatacgtg tcatgaaatg cgcagtagaa gacaatgata ccgctcataa
    43261 tctgcactgg cagttagttg tacgcaaatc aagtacaata gaagaataaa aacagataaa
    43321 atgttgagag cataaagagg ttgtgagtct cccgtttagc tctgagttac aaggaataat
    43381 gttaaaaaac agcttttcat gttttatgaa tattgccttg tgaccgaggt gttggcagga
    43441 gaatactgtt tatttgaaaa aagggctgcg acaagacttt tgtcacagcc tttattttca
    43501 tgaataggag ttacttttgg ctacttttag agaagtagct tttaaggagc agataaagtc
    43561 aaggctttta tttctataaa tgaagtagta aaatgaagaa cagattttat tacaggaagg
    43621 atgccgataa ttgtgatcat tgttgcagga acgatcggtg cagggaaaag cacactaact
    43681 gaaatgcttg cccaagattt agaaacaaaa cctttttatg agaacgtgga agataatgaa
    43741 gtattgccgc tattttattc taatccagaa aagtatactt ttttattaca gattttcttt
    43801 ttgaacaaac gctttttggc aatcaaagat gctttcagcc atgatgacaa cgtactagac
    43861 cgctccatct acgaagatag tatgctgttt catttgaatg cagatttagg gcgtgtttct
    43921 gaagtagaag taaagcagta tgaaggtctt ttagaaacga tgctgaagga gttagaagaa
    43981 atttcgccac aaaagaaacc agatctactg gtttatattc gtgtatcctt tgaaactatg
    44041 ttagcgagaa tcaaaaaaag aggccgggaa tatgaacagc tagaacaaga tcctgaactc
    44101 tattcctact ataaagaact caatcgacga tacgaagagt ggtatgaaca gtttgatata
    44161 tgtcccaaaa tagtgatcga tggggacaaa tatgatttcg tagccgatcc ggcatgtgga
    44221 gaacaaatcg tacaagaaat caagatgcgg gcaaagaaaa tgacagaaga ggcagatgcc
    44281 cagtcgatcc gctacactac aaataacccg aaagcaaagg gaatctttac aactaaataa
    44341 tataaaaact aagcaaattg ctcactcgca gatatttctc tgtgagcagc atcttgctta
    44401 gtttttttga ttcattgaga tcaaatagtg aaccgattcg tatgataaaa ttgccataaa
    44461 tgaaatgtct gagtgagggc catgatcaaa aataaaatgg aaacgatcaa aatcgaatgg
    44521 gcgccaaaca gatgatgaca gaaaacaagg acaatagctc ctgcgataaa ggcaagaacg
    44581 acacttagat agtggaaaaa tcgatcaaaa tctgcttttt ctttagaaat acaataatca
    44641 tataagttcg ttgctacccc ttttaaattt ccagttgtaa ataaattagt gtaggcacgc
    44701 ccgttgattt tatcaaaagc tacccactgg atcgatgcag taaaagcaat caaaatcgtt
    44761 actaatgcgg gatgctcatg gacgggtgtc aaactaacaa agaaaaaaat cactgcttca
    44821 taatatagaa caaacaagcg ccagaaatgt ttttcttttt ttctaaaata atcaattaga
    44881 tactttgtta gaagaatccc taagaaaaag aaaagggtag acaatacttt tttaccaaca
    44941 cttagccatt ctccgtcaaa tgcttggatg atcgatagaa tgatattgcc agtttgagca
    45001 gaggcaaaag caccatattg gatataagta taactgtcca tggctcctcc gataaatgtc
    45061 agcaaaagtc ctattgtacg cccctcgtga aaggaagtct gcattttttt catcaccttt
    45121 tatgaatatt tttgtcttcc ttatcttagc ataaacgatt gaaaaaaagg aaaaattttc
    45181 agaaaactaa aatcatatcg cttcccattt tcatttttag aaaggtctgt tagtataaag
    45241 aaaaaataga caacgaaact ttaaataacc aacttgaaat aaacaaattc aaagaagaaa
    45301 gatgggaagg aaatcaatca gttgaatgaa aaagaaattc tccgattaca aaaagaattg
    45361 aaacgctggg agaacaagta taaaaagctt caacaagtct ttcggctaca agaagaaatc
    45421 attgctacct atgaagcggc ttatggcgaa gcacccggac ttattgagga agaagtacaa
    45481 gtaaaagaaa gtgaaggaaa cagctctttc tttaaaatga tggagaaacg ttcctataat
    45541 cgcttcaatc aagaacaaaa agcagctatc acatatgata tgaagaaaca tcttcgaatc
    45601 atagcaggag ctgggagcgg caaaacgcaa acaatctgtg caaaagctgt ttatctgatg
    45661 acgcaaaaac aagtagacga agaacgcata ctaatgatca cttttacaag gaacgctgca
    45721 aatgaactaa aaaaacgagt agataatttc agccagagaa aaaccgctgt ccatatcgga
    45781 acattccata gtatcttttt taggatatac aatgaaattt gtcgaaaatt tcctgaagta
    45841 gcaatgcaag gaatacaagg agacttttca aaggatactg cacaaaaagt gaatgctgtt
    45901 ttgcaacagc tgatacgtaa atataatctt tatatatttg atcaatatgg agaaaaaacg
    45961 attgcttctc ggttagacta ttggcagaat atgaaccttt cagtagaaga aatggttcag
    46021 ttagttaaag aaaaatatga ttccattgat aagaatgcac gacagccgat atcagaacga
    46081 ctgtatggat tgctcacgga actccaagaa caaaaaagaa gccagcaatt gctagagttc
    46141 aatgatgtac tccaaaatct gaaaagcgct ttggagaatg aggagatcca gcattatatc
    46201 ggccaaaaat atgattatct attcattgat gaatttcaag atactaatcc acttcaatgg
    46261 gaaatcgtga aacggatgac aaaggaaaac aggatcaagt tgattgttgt tggagatgat
    46321 gatcagagta tctatggatt cagaggatca gagccagcat acatcaagaa ctttgaaaaa
    46381 gaatatccta ccaaaactct tttcttatta actaattatc gttctcgggc agctattgtc
    46441 caaggagcaa atagattgat cagctataat aaaaatgatc ggataccaaa ggcgatgatt
    46501 ccagcacaaa aagaagcagg tgttatggaa gcctatttgt tttctgatac taaagcagaa
    46561 agtcagtggc taatcaaaca aattcagaca tttattcaaa aaaatgggaa atataaggaa
    46621 tcaatcatat tataccgatc agcttcacaa acgcagcagc ttgtccaatc tctgctgaaa
    46681 acgaatattc cttttgtact ggaagcagat agtccatatg aaggtatctt tggtatcaag
    46741 tctttccaac atttctatca aaaaattgtt cattggcaaa cagcactgaa tctgcaaaag
    46801 aaaaatcagg cctaccagca attgctccgg cagctgatgg cagactgcta cctgaaaaaa
    46861 agtgaatgcg atcaatactt ttctcctaaa tatcaaaata tagccgttgc agattatatt
    46921 ttgtcgataa gccccaattt aaaatcgaaa tctgcagaaa tccgagagtt tgaggaagcg
    46981 ttgcgtaaaa cagaccagca tcaagaaatc tattcgcttg ttcaaagcta tcttcatctg
    47041 ccacgtatag ctaaggaaat cgaccagagt gatcaagaat ggatacgaga agaagtaaag
    47101 cagcatgcta ctttcagaag actccaagcc ttatcgcaag aaacccaagc gacagctaaa
    47161 gaattaaaaa cccgcttgat tgcttacaga caaggaaaac ttgatgcact atgtatccag
    47221 agcattcaca aaagtaaagg tcttagttac cgaaatgtct ttttgattgg ttgtaacgaa
    47281 ggaagtttgc cttataacgg agcgacagaa aagaaagtaa tcgactttaa tgaaataaaa
    47341 gcagaaccag taacgacgat agaagaagaa cgaagactat tttatgttgc gatgactaga
    47401 gcaagaaatc gtctgtatct atgtgtacct caaatgaaaa attcaagaaa attgaaagta
    47461 tcgagattta tcaaagaaac cgggagtcgt gtgaaaaaag gaaagtgata gaagagaggg
    47521 tgtgacagaa gtggtcagtt tcaagaaaga agaaggaatg tacgaaaatt gcttctcaaa
    47581 tttttgtgaa atttcagctt attttcgaag aagctatttc tggaacaca
//
LOCUS       DAZKNM010000006        44009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000006 DAZKNM010000000
VERSION     DAZKNM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.006"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG8M9_000259"
     CDS             6..245
                     /locus_tag="ACG8M9_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377065.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG8M9_000260"
     CDS             312..521
                     /locus_tag="ACG8M9_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377066.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG8M9_000261"
     CDS             581..1252
                     /locus_tag="ACG8M9_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0377067.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG8M9_000262"
     CDS             1305..3281
                     /locus_tag="ACG8M9_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0377068.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG8M9_000263"
     CDS             3294..4049
                     /locus_tag="ACG8M9_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0377069.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG8M9_000264"
     CDS             4046..4870
                     /locus_tag="ACG8M9_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0377070.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG8M9_000265"
     CDS             4880..5128
                     /locus_tag="ACG8M9_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377071.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG8M9_000266"
     CDS             5171..7270
                     /locus_tag="ACG8M9_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0377072.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG8M9_000267"
     CDS             complement(7308..9365)
                     /locus_tag="ACG8M9_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0377073.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG8M9_000268"
     CDS             complement(9479..9691)
                     /locus_tag="ACG8M9_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377074.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG8M9_000269"
     CDS             9878..10345
                     /locus_tag="ACG8M9_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377075.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG8M9_000270"
     CDS             10342..10584
                     /locus_tag="ACG8M9_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0377076.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG8M9_000271"
     CDS             10602..10904
                     /locus_tag="ACG8M9_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377077.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG8M9_000272"
     CDS             10976..13030
                     /locus_tag="ACG8M9_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377078.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG8M9_000273"
     CDS             13030..15642
                     /locus_tag="ACG8M9_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0377079.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG8M9_000274"
     CDS             15639..18170
                     /locus_tag="ACG8M9_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0377080.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG8M9_000275"
     CDS             18222..18554
                     /locus_tag="ACG8M9_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377081.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG8M9_000276"
     CDS             18555..19148
                     /locus_tag="ACG8M9_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377082.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG8M9_000277"
     CDS             19311..21005
                     /locus_tag="ACG8M9_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0377083.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8M9_000278"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8M9_000278"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0377084.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG8M9_000279"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG8M9_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG8M9_000280"
     CDS             24078..25283
                     /locus_tag="ACG8M9_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0377085.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG8M9_000281"
     CDS             25299..25943
                     /locus_tag="ACG8M9_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377086.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG8M9_000282"
     CDS             25954..26760
                     /locus_tag="ACG8M9_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377087.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="ACG8M9_000283"
     CDS             26782..27012
                     /locus_tag="ACG8M9_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377088.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="ACG8M9_000284"
     CDS             27558..27833
                     /locus_tag="ACG8M9_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377089.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="ACG8M9_000285"
     CDS             27875..28297
                     /locus_tag="ACG8M9_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377090.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8M9_000286"
     CDS             28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8M9_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0377091.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="ACG8M9_000287"
     CDS             29920..30255
                     /locus_tag="ACG8M9_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377092.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="ACG8M9_000288"
     CDS             30402..30596
                     /locus_tag="ACG8M9_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377093.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="ACG8M9_000289"
     CDS             30628..33000
                     /locus_tag="ACG8M9_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0377094.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="ACG8M9_000290"
     CDS             33061..34521
                     /locus_tag="ACG8M9_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0377095.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="ACG8M9_000291"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="ACG8M9_000291"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0377096.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36752..36901
                     /locus_tag="ACG8M9_000292"
     CDS             36752..36901
                     /locus_tag="ACG8M9_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377097.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36975..37568
                     /locus_tag="ACG8M9_000293"
     CDS             36975..37568
                     /locus_tag="ACG8M9_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0377098.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="ACG8M9_000294"
     CDS             37584..38483
                     /locus_tag="ACG8M9_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0377099.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8M9_000295"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8M9_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0377100.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="ACG8M9_000296"
     CDS             39337..39597
                     /locus_tag="ACG8M9_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377101.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="ACG8M9_000297"
     CDS             40038..40244
                     /locus_tag="ACG8M9_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0377102.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="ACG8M9_000298"
     CDS             40244..40495
                     /locus_tag="ACG8M9_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377103.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="ACG8M9_000299"
     CDS             40510..40947
                     /locus_tag="ACG8M9_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377104.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="ACG8M9_000300"
     CDS             40940..41647
                     /locus_tag="ACG8M9_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377105.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41812..42012
                     /locus_tag="ACG8M9_000301"
     CDS             41812..42012
                     /locus_tag="ACG8M9_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377106.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42028..42207
                     /locus_tag="ACG8M9_000302"
     CDS             42028..42207
                     /locus_tag="ACG8M9_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377107.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42548..42811
                     /locus_tag="ACG8M9_000303"
     CDS             42548..42811
                     /locus_tag="ACG8M9_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0377108.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42805..43155
                     /locus_tag="ACG8M9_000304"
     CDS             42805..43155
                     /locus_tag="ACG8M9_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0377109.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43179..43793)
                     /locus_tag="ACG8M9_000305"
     CDS             complement(43179..43793)
                     /locus_tag="ACG8M9_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0377110.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT        16157 a         6975 c         8960 g        11917 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat tttttatatt cacgaagata aggtcaaaga tcacgtgaaa
    26461 gacgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 accttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacttcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ttctaaagct acccgaaaag atgggtattt
    34621 tgagatccaa gatcgattgt ttacaggaga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgagtta gattcgcccg atatgtacga tgaaaaatgg aaacaatggg cattagaaca
    34741 tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga aaaagcagtt
    34801 tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga ctgatagcga
    34861 tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct atatgattta
    35161 tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag gaaaagccca
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa cccaagcgac
    35281 acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa aacagccagg
    35341 tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc ttttttcttt
    35401 atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag atactttgga
    35461 agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg atacccctta
    35521 tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca agcatttttt
    35581 aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg tgaataataa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggctat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaagtata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct
    35821 tcagttaaag gcaatcgaaa aggtgccgct agatctaggt tggaagaagt tatttggaaa
    35881 agagagcgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtggatctac aagttcttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
    42301 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
    42361 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
    42421 atataaatgt taacatagtt tatggattca tggaatgtta atctaattta atagaaaagg
    42481 ataattaagt atatacgttt tttcttatgt atgttatact atgtatatac agaaagaggt
    42541 gtctactatg gaaatgttac ctataaaaaa atggggaaat agtaatggcc ttagattgcc
    42601 aaagtatatt atggaatatc ttggaattca tacagaagac aaagtgaaga taatacaaga
    42661 agaagtaaac ggtcagaagc ggctaattat cgaagcggct aatatagaaa atgaattaac
    42721 aattgaacag ttatttgaaa attacactga tgaaagaaat catgtgacga ttcaaaattt
    42781 aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta gacaaggcga tattctatta
    42841 ttgaatacag cacctcgatc tggacacgaa cagacaggta aaagacctta tattgtttta
    42901 agtcatgata ttattgcgga ttatagtaac gttgttacag ttgctccaat ttctagtaca
    42961 actagaaact atccgctata tgtaaatata aactcagaat atgaaatgaa gacaacagga
    43021 aaagtattat tagatcaact aacgacaatt gattatgaag ccagagagtg tatttttttg
    43081 gaaaaagcgc atgatagttt agttgaggag ttattgatga aagttagaac agtttttcaa
    43141 aaagtaagca aataaaatag aaacagcagc atgatcaact attcgtgatt gaccatgctg
    43201 tcttttttta ttctatatac ggtttgtctc gtaacattgt agtcttttgc aatcttagcg
    43261 atcgcaacac ctttttttag atcctcaacg atgtttttat aaacaagacg acgttgggga
    43321 tcttttgcat tggcactgta tagcttagga cgtcctttat aaacaccgtt cgcttttgca
    43381 atcttaatgc cttgtgcctg acgtcgttta gattcggtac gttcttgttc agcgatcatg
    43441 gccaaaattt gaatgatcaa atctttaatg aatttatcca ggagaggatt gccaattgct
    43501 tcggccatga tcggtagact tgtgatgatc agctgcacat ttttcttctt caagtaattg
    43561 actgaagcga tgatctcgtc gtaatttctt ccaagtcgat caatagcttc cacaataaaa
    43621 atatcttgat ctcggacgaa atccagtgct tcttgaaaaa caagtcgatg agtaatcgtt
    43681 gcgccagatt gtttctcaat gaaaattctc tctgcaccga attttttcat ttcttcaatc
    43741 tgccgttgtt cattttgatc gatggatgag acacgtatgt atgcaactct caaaattttt
    43801 acctactttc tgcaactatt taaaaaacac cctaatcata cacgagaaaa ccagattttt
    43861 caatgtcgcg gtcgtgtcta gttagggtat accttatata gacaaaaact aatgatggag
    43921 gaaatagtat gtgtcacttt tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat
    43981 caagaaaatt taaacgatta tgacattaa
//
LOCUS       DAZKNM010000007        42438 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000007 DAZKNM010000000
VERSION     DAZKNM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.007"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..429
                     /gene="nrdH"
                     /locus_tag="ACG8M9_000306"
     CDS             205..429
                     /gene="nrdH"
                     /locus_tag="ACG8M9_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0377111.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            588..2747
                     /gene="nrdE"
                     /locus_tag="ACG8M9_000307"
     CDS             588..2747
                     /gene="nrdE"
                     /locus_tag="ACG8M9_000307"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0377112.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2797..3762
                     /gene="nrdF"
                     /locus_tag="ACG8M9_000308"
     CDS             2797..3762
                     /gene="nrdF"
                     /locus_tag="ACG8M9_000308"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0377113.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3851..4990)
                     /locus_tag="ACG8M9_000309"
     CDS             complement(3851..4990)
                     /locus_tag="ACG8M9_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0377114.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5299..6012)
                     /locus_tag="ACG8M9_000310"
     CDS             complement(5299..6012)
                     /locus_tag="ACG8M9_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0377115.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6266..6967)
                     /gene="nagB"
                     /locus_tag="ACG8M9_000311"
     CDS             complement(6266..6967)
                     /gene="nagB"
                     /locus_tag="ACG8M9_000311"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0377116.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7201..8733
                     /locus_tag="ACG8M9_000312"
     CDS             7201..8733
                     /locus_tag="ACG8M9_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0377117.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8924..10126)
                     /locus_tag="ACG8M9_000313"
     CDS             complement(8924..10126)
                     /locus_tag="ACG8M9_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0377118.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10208..10897)
                     /locus_tag="ACG8M9_000314"
     CDS             complement(10208..10897)
                     /locus_tag="ACG8M9_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0377119.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(11003..11371)
                     /gene="rplL"
                     /locus_tag="ACG8M9_000315"
     CDS             complement(11003..11371)
                     /gene="rplL"
                     /locus_tag="ACG8M9_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0377120.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11424..11927)
                     /gene="rplJ"
                     /locus_tag="ACG8M9_000316"
     CDS             complement(11424..11927)
                     /gene="rplJ"
                     /locus_tag="ACG8M9_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0377121.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(12131..12258)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12337..13026)
                     /gene="rplA"
                     /locus_tag="ACG8M9_000317"
     CDS             complement(12337..13026)
                     /gene="rplA"
                     /locus_tag="ACG8M9_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0377122.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(13130..13552)
                     /gene="rplK"
                     /locus_tag="ACG8M9_000318"
     CDS             complement(13130..13552)
                     /gene="rplK"
                     /locus_tag="ACG8M9_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0377123.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13895..14563
                     /gene="sdaAB"
                     /locus_tag="ACG8M9_000319"
     CDS             13895..14563
                     /gene="sdaAB"
                     /locus_tag="ACG8M9_000319"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0377124.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14576..15448
                     /gene="sdaAA"
                     /locus_tag="ACG8M9_000320"
     CDS             14576..15448
                     /gene="sdaAA"
                     /locus_tag="ACG8M9_000320"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0377125.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15539..16639)
                     /locus_tag="ACG8M9_000321"
                     /pseudo
     CDS             complement(15539..16639)
                     /locus_tag="ACG8M9_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(16731..17618)
                     /locus_tag="ACG8M9_000322"
     CDS             complement(16731..17618)
                     /locus_tag="ACG8M9_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0377126.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17695..18240)
                     /gene="nusG"
                     /locus_tag="ACG8M9_000323"
     CDS             complement(17695..18240)
                     /gene="nusG"
                     /locus_tag="ACG8M9_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0377127.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18341..18511)
                     /gene="secE"
                     /locus_tag="ACG8M9_000324"
     CDS             complement(18341..18511)
                     /gene="secE"
                     /locus_tag="ACG8M9_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0377128.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18535..18687)
                     /gene="rpmG"
                     /locus_tag="ACG8M9_000325"
     CDS             complement(18535..18687)
                     /gene="rpmG"
                     /locus_tag="ACG8M9_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0377129.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18849..19493
                     /gene="cbpA"
                     /locus_tag="ACG8M9_000326"
     CDS             18849..19493
                     /gene="cbpA"
                     /locus_tag="ACG8M9_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0377130.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19549..20052)
                     /locus_tag="ACG8M9_000327"
     CDS             complement(19549..20052)
                     /locus_tag="ACG8M9_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0377131.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(20065..20111)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20649..21614
                     /locus_tag="ACG8M9_000328"
     CDS             20649..21614
                     /locus_tag="ACG8M9_000328"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0377132.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21713..22885)
                     /locus_tag="ACG8M9_000329"
     CDS             complement(21713..22885)
                     /locus_tag="ACG8M9_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377133.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22872..24017)
                     /locus_tag="ACG8M9_000330"
     CDS             complement(22872..24017)
                     /locus_tag="ACG8M9_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377134.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(24031..24955)
                     /locus_tag="ACG8M9_000331"
                     /pseudo
     CDS             complement(24031..24955)
                     /locus_tag="ACG8M9_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            25072..26151
                     /locus_tag="ACG8M9_000332"
     CDS             25072..26151
                     /locus_tag="ACG8M9_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0377135.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            26148..26776
                     /locus_tag="ACG8M9_000333"
                     /pseudo
     CDS             26148..26776
                     /locus_tag="ACG8M9_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(26886..27113)
                     /locus_tag="ACG8M9_000334"
     CDS             complement(26886..27113)
                     /locus_tag="ACG8M9_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0377136.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(27216..29804)
                     /locus_tag="ACG8M9_000335"
     CDS             complement(27216..29804)
                     /locus_tag="ACG8M9_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0377137.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(29824..30483)
                     /locus_tag="ACG8M9_000336"
     CDS             complement(29824..30483)
                     /locus_tag="ACG8M9_000336"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0377138.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(30532..31035)
                     /gene="trmL"
                     /locus_tag="ACG8M9_000337"
     CDS             complement(30532..31035)
                     /gene="trmL"
                     /locus_tag="ACG8M9_000337"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0377139.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(31256..32122)
                     /locus_tag="ACG8M9_000338"
     CDS             complement(31256..32122)
                     /locus_tag="ACG8M9_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0377140.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            32231..32872
                     /locus_tag="ACG8M9_000339"
     CDS             32231..32872
                     /locus_tag="ACG8M9_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0377141.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(32934..34295)
                     /gene="mgtE"
                     /locus_tag="ACG8M9_000340"
     CDS             complement(32934..34295)
                     /gene="mgtE"
                     /locus_tag="ACG8M9_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0377142.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(34313..35212)
                     /locus_tag="ACG8M9_000341"
     CDS             complement(34313..35212)
                     /locus_tag="ACG8M9_000341"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0377143.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(35214..36011)
                     /locus_tag="ACG8M9_000342"
     CDS             complement(35214..36011)
                     /locus_tag="ACG8M9_000342"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0377144.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(35989..36693)
                     /locus_tag="ACG8M9_000343"
     CDS             complement(35989..36693)
                     /locus_tag="ACG8M9_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0377145.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            36824..37400
                     /locus_tag="ACG8M9_000344"
                     /pseudo
     CDS             36824..37400
                     /locus_tag="ACG8M9_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            37518..38195
                     /locus_tag="ACG8M9_000345"
     CDS             37518..38195
                     /locus_tag="ACG8M9_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0377146.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(38274..40082)
                     /gene="pepF"
                     /locus_tag="ACG8M9_000346"
     CDS             complement(38274..40082)
                     /gene="pepF"
                     /locus_tag="ACG8M9_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0377147.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(40258..41256)
                     /locus_tag="ACG8M9_000347"
     CDS             complement(40258..41256)
                     /locus_tag="ACG8M9_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0377148.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(41482..42135)
                     /locus_tag="ACG8M9_000348"
     CDS             complement(41482..42135)
                     /locus_tag="ACG8M9_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0377149.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        12829 a         8832 c         7484 g        13293 t
ORIGIN      
        1 tcatagtttt ccacaatttt ttctctatta aatttgctat tttcccacac gatacttgtt
       61 ttcagctctt ttttcaaaaa cacaaaactt agtattccac agtttccaca acacaatatg
      121 tagtgtttgt tgtttgactt ttttactaag agttttatac tatctattat agataataag
      181 aatgtgttag aggaggcact caccatgaac gtaaaaatct tttctaaaaa taattgtatg
      241 caatgtaaga tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat
      301 atcgatgaac agcctgaagc agtagactgg ttgaaagcac aaggattcca aagtgttcct
      361 gtcattactt ctgatgcaga aacagttatc ggattccgtc ctgatcagtt gcgtaaactt
      421 gccagctaac atgactaaaa gaatcaaaac gtgatacatg gatttgaagg gcttgagaca
      481 gcaccagttt ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac aatgcaatga
      541 tttgcaatca agcatcatca aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa
      601 atttacaaga tgtcagttat ttcaagctga ataatgaaat caatcggcca gtcaatggtc
      661 aaatcccttt gaataaggat aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac
      721 caaatacgat gacttttcta tctattatgg aaaaaatcaa ttacttgatc gatcaagaat
      781 atatagaagc agaattcatt caattgtatt caccatcatt tattgaaaag ctatatcaat
      841 ttttggacga gcaacatttt tctttcaaat cttttatggc tgcttacaaa ttttattctc
      901 aatatgcgtt gaaaaccaac gacggttccg cttatctgga aacttatgaa gatcgagtag
      961 ccttcaatgc gctttacttt gcgaacgggg atgaagaatt agcacttgct cttgcagacg
     1021 aaatgatcca tcaacgatat cagccagcta caccttcttt cttgaatgct ggacgtaaac
     1081 gtcggggcga attggtgtct tgtttcttga ctcaagtcac tgacgatatg aactctatcg
     1141 ggcgctcaat caactctgct ttgcaattgt cccgtattgg tggcggcgta gggattactt
     1201 tatcaaatct tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct gcaagtggtg
     1261 tcgttccagt catgaaatta ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc
     1321 gtcaaggagc tggcgtggtt tatttgaacg ttttccatcc agacattatt gctttccttt
     1381 cagcaaaaaa agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga
     1441 tcgtacctga taaattttat gaattgacac gtaaaaacga agatatgtat ttatttagtc
     1501 catatagtgt cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt acagctgagt
     1561 atgatcatct agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg
     1621 aaaatgaaat ctcaaaatta caacaagaat ctggctatcc ttacgtaatc aatattgata
     1681 cagcgaaccg cgagaatcca atcgatggca agatcatcat gagtaattta tgttcagaga
     1741 ttttacaagt tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa gtattaggaa
     1801 cggatatctc atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa agccctaact
     1861 ttggtcaatc tgtgcgtacg atgactcgtg cattgacatt tattacagat gcatctgata
     1921 tcgatgtcgt accaccgatc caaaacggaa ataagctgaa tcatacgatt ggattaggtg
     1981 ccatgggctt acacacttat cttgcaaaag aacaaatgga atatggctca gaggattcat
     2041 tagactttac aaatatttac ttcatgctgt tgaattattg gacacttgtt gaaagcaaca
     2101 atatcgctcg tgaaagaaaa caagttttcc ataattttga aaaatcagct tatgctgacg
     2161 gcacttattt cgaaaaatac gtaactggag aattccagcc aaaatcagaa aaagtcaaag
     2221 cattgtttga aggcattttt atcccaactg ctgaagattg gaatgctttg aaacaagcag
     2281 taatgaaaga tggactgtat catcaaaatc gcttggcagt agcaccaaat ggctcaattt
     2341 catatatcaa cgatacgagt gcaagtattc atcctatcac tcgcatgatc gaagaacgcc
     2401 aagagaagaa aatcggtaaa atttattacc cagcgcctta tctatccaat gagacgattc
     2461 cgtattacac ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt tatgcaatgg
     2521 cacaacaaca cgtcgatcaa ggcatgagtt tgactttatt catgcgttct gaaatcccag
     2581 aaggcattta tgaatggaag acaacaacga agcaaacgac acgtgatttg aatattttaa
     2641 gacattacgc tttccacaaa ggaatcaaat ccatttatta cgtacgtacc tttactgatg
     2701 atgcagaaga aatcggaagc aatcaatgtg aaagctgcgt gatctaatat catagataga
     2761 ttcgcttatt catattttaa ttaaaggagc attctcatgt cagaaacata ttatgaagca
     2821 ataaactgga atgaaattga agatatcatt gataaatcca cttgggagaa gctgacagag
     2881 caattttggc tagatacacg tatcccactt tcaaatgacc tagatgattg gcgtacattg
     2941 tctcaattgg aaaaagatac agtaggacat gtatttggcg gtttgacact gctagatact
     3001 gtccaatctg aaagtggcat ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa
     3061 gccgttttga acaatattca attcatggaa tccgttcatg ccaaaagtta ttcttctatt
     3121 tttagtacac tgaatacgaa aaaagaaatc gaagaaattt ttgactggac aaatacgaat
     3181 ccttatctgc aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg tacaccgcta
     3241 gaaaagaaaa ttgccagcgt ctttttagaa acattcttgt tctattcagg tttttatact
     3301 cctctttatt atttaggaaa caacaaattg gctaacgtag cagagatcat caaactaatc
     3361 atccgagatg aatctgtaca tggaacctac atcggttaca aattccaatt aggattcaac
     3421 gagttacctg aagcagaaca agaaacgtta aaagactgga tgtacaatct tctttatgag
     3481 ctttacgaaa acgaagaacg ttatacagaa gaattatacg atccaatcgg ctggacagaa
     3541 gaagtcaaga cattcctacg ctataatgcc aacaaagcat tgatgaacct agggatggat
     3601 ccactctttc cagatactgc caatgatgtc aacccaatcg taatgaacgg gatttcaaca
     3661 ggcacaagca accatgactt cttctcacaa gtcggaaacg gctatttgtt aggtcatgta
     3721 gaagcgatga aagacgacga ttatttgatt ggattggaat aaggatttat tttaattcaa
     3781 aaatttttcg aaaaataaaa agagtttagc gtacagatat tttaaatgta cactgaactc
     3841 ttttttatgc ttattcactt gtttcttcca gcacttttgg actgtctaac ttttccgaaa
     3901 catgttccat tcttctcaat aaatagattt tccggatatt taatattatc gtcttaatca
     3961 ctgcatagac aggtatgcaa atcagcatgc ctaatattcc tgcgacactt cctgctccaa
     4021 tcaataccaa gatgatcgtc aaaggatgga tattcattga tttcccaaat aacaacggct
     4081 tgatcaaatt tccatctaat tgttggatca ctaaaatcga caacgccata tacaaagctt
     4141 gaaatggaga gataaacaaa ccgacaatga ttgctggtac cgctccaata aaagggccga
     4201 catacgggat aatattcgtg attccgcaaa tgaccgcaag caacaagcta taaggctgat
     4261 cgaatattct cattgccaaa aagctcatta caccaataat caaagcatct aagatcgtac
     4321 tgctgatata ggcagataat gtttcgttta attcttttac cgtttggcgt aactctttac
     4381 gaatcgcttt cggaaagaag tgagacaacg catcaataaa tttgtgccca tccttgaaca
     4441 taaatagcaa aataaatgga acggtaaata acaaaataaa aaattgcatc ataaatgaaa
     4501 tgatttttga gacacttgca gataaactga ctaatatgat attgaacaaa ttactaagag
     4561 ataaatttgc tgatgctaat tgttcttcca aattgaaatt tttaaactct tcccgctgcg
     4621 ccaattcatt taaccatttg cttgtttcat ctgcatacat tggcaagctc tgggtcaatt
     4681 gaattgtctg ttccattaac tgaggcacta cattcatgac agttaaaaca actaaaatga
     4741 ttacgaccaa aaacgtcaat aaaaaaccga acactctcgg gatccgatgc ttttcgagaa
     4801 aaacaacgat tggatcgaac atataataga taaaaccagc aacaagcaat ggtaaaaaaa
     4861 ctgccgaaag cattttcaat attggactaa aaatattcgg catttgtaaa ataaagtaca
     4921 cgccgatgat cgtgagtact agctccactg tccagaaaaa taattttgat tgtttaaaac
     4981 gtgattgcat acatctcctc caaggtcctt tgatacttct attctatcat ttaacaaaga
     5041 gttacgcgct gagattttca tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg
     5101 agtagacgag tacttaaaca aaaacacggt ttggagtagt gacaaaaatt atactgcagt
     5161 ccctttttcc aaataaacgg tgttcaaaag cttgcatctt cgtaataagc ccagccaaac
     5221 gaaaaatata aggaactatt tttatttggc tgggcttatt acttgatgta ggacatctat
     5281 tttacaacct cagatcattt attgcgcaat actgactaaa tgcaactttg tgatattttt
     5341 gtgtacggac aactcctcga tcacatcggc ataagtgagc ccttttggaa gatcaatcgt
     5401 atagatattt ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc
     5461 gattacgata tttttctctt caaaataatc ggtaagaaaa tctttggttt cttttctatg
     5521 gataaattgg atttctaact ttttagtcgt aggtacatgg atcacgcgtt tcactactgt
     5581 gagtgcaata gcaatcccga taaaactgat gatcgcaatg gaatagtaac ccatgccgat
     5641 ggcgatccct aatccagcaa ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga
     5701 ttgtttcgtg acgatgatcg ttcccgcgcc taagaaaccg acaccactca ccacttgagc
     5761 aatcagcctt gcttcatctg aacgaatcgt tccagcaagc tgtggatact ctaaagcatc
     5821 tcttaatgca ccactagcga tctcaacttg gatcaatgca atgatacatg ctcccataca
     5881 aactaaaata tgcgtacgca tacctgctgg acgattttta tattgtcgtt caaaaccgat
     5941 tgctccgcca aatagcatag cgatccctaa acgtaaaata atctctggaa ttgtcaatac
     6001 catatcattc atctaaacac cctccagtcc aatatatcat gtctgaaagg agcagacaaa
     6061 aaaagtgcaa tttttacctc ttacgaaaaa aggactgtga caaaattttt gtcatagtcc
     6121 tttttatgaa taaacggtgt tcaatcagct gaccctcggt attactcaac aaacggcaaa
     6181 atatggggag ctatttttcc tttgtcttct ctaatactca ggtcaaaacg ctgatttcac
     6241 aacctcttta gcctactatt gaaaattaca acttacttgc tgcttcttca tcgatgatca
     6301 caacaacatt gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt acaggtcctt
     6361 caatcatccc tttgattgca tcagctttgc tttcgccaaa tgccatcaag acgatttctt
     6421 tacttttcat aattgaaccg attcccattg aaacagcttt tgttggtaca tcttctgctt
     6481 tatcgaagaa tcgtttgttt gcattgattg ttgattctgt taagtcaact actgaagtcg
     6541 ttccgtcaaa tgaagcacca ggttcattga aaccgatatg tccattttgc ccgattccta
     6601 agatttggat atcgattgga tgtgcatcga tgatttgatc atactttttg catgctgctt
     6661 ccagatcttc tgcttttcca tctggtacaa acgtttcttt gaatggtttt ttatcaaata
     6721 attggacatc catgaaatga cggtagcttt gttcatctga accgcctagt ccaacgtact
     6781 catctaagtt tacagaagtc atttcactga aatccaaatc gcttgaagtc atttctttat
     6841 ataaagtaac tggtgtgctt cctgttgcca atcctaatac ttttgcacct ttgttcatac
     6901 cttctttgat gatttcaaat gctttttttc cgccttcttc agcgtttttt acacgaatga
     6961 tttccatagt gattgctcct ttatattttg gtatagccca attatagcat tctttttttc
     7021 tttttacaat aattagtata gaccaaattg catttttttc attttcttga caataaatag
     7081 gaatccgtgt taggtttcat aagatggttc aatcaaacaa tccatgttgc atgaaaacca
     7141 agcaagtgat tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa aggagttatt
     7201 atgagtatac tgacattaaa caacattacc cagcaatttg gcgacaaaat cctctatgaa
     7261 ggtgtcgatc tgcaattaaa tgctggcgaa catcttgggc tgattggaca aaacggagcc
     7321 ggtaaaagta cattgattaa aatcatcact ggtgagatct taccagatga cggacagatc
     7381 cattggcaaa aaaatattca caaaggttac ctcgatcaat atgtggaagt cgatgaaaca
     7441 ttaacaatcg aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa
     7501 attgcttttc tttaccaaac atatagcgag accttcgatg attccttgct ggaaaaagct
     7561 gggaaactcc aaacggaatt agaccaagga gtgttttatc aaatcgacac cttagttgct
     7621 gagatgagca gcgggttggg gatcgatgtc ttaggtttag attcactgtt gaaaaatcta
     7681 agtggtggac aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa cccagatgtt
     7741 ctgatcttag atgaaccaac caatcactta gatgatcagc atatccaatg gctgacgcag
     7801 tttcttcagg attttgacgg tgcgtatatc gtgatttccc atgaccaaga atttcttgat
     7861 cagatcacca cccatatcgc agacatcgag tttggaaaga ttactaagta tactggccat
     7921 ctaaaacaag cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat
     7981 gctcaacaaa aacaaatcga aaagaccgaa gcttatattc gaaaatacaa agccggttcc
     8041 cgatcaacga tggcaaaaag ccgacaaaaa caattagata aaatcgaacg gctgacgcct
     8101 ccagcttcag caagtaaagc tgtctttgat tttccattct cgcctattgt cacgacatta
     8161 gcagtggaaa caactgattt agtcatcgga tatgatcgac cgctacttgc acccatcaat
     8221 ttgaccatcc gctttggtga aaatattgcc attcgaggct tcaacgggat tggaaaatct
     8281 actttattaa aaacactgat cggtgagatt gaaaaaataa gtggcgattt ccacttccca
     8341 gataacacaa aaatcaatta cttttcacaa gatctgtact gggaaaatcc attagaaaca
     8401 ccactacaat atttaagcag tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa
     8461 ctagctaaag cagggttagt taatcaatta gcaagtgaac ctctcactac attaagtggt
     8521 ggggaacaaa ctaaggtgaa acttgcacag ttgacaatga accaaggtaa tttactgatt
     8581 ctcgatgaac cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca agacgggata
     8641 cagaaatttc caggaacagt aatcatcgtc tctcatgaac aagaatttta tcaagatctt
     8701 gtgcaacgag tcattgagat tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag
     8761 gagctgtgac aaaagttttg tcacaactcc ttcttctgaa taaatggtgg ccaattagct
     8821 gaccttcggt attagctcaa aaaacggcaa aatatgagga gctattttta tttggctatt
     8881 cttattactt gatgcagaac agcttttcac aacctctttt cgtctatttt ttattttcga
     8941 gatttctctt tcctttaaaa acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa
     9001 gcccgctaac cgttacacct aaccagccat actgctgcca cattgtcgtc cctagcaatg
     9061 aaccagccgc acctccaata aagtacataa acataaatac agtattattt cggttgcttg
     9121 ctgcttcacc tagcccctgt actcgtgttt gattggcgac ttgaccaaat tgattcccga
     9181 cgtctaataa aataatagct aaaagcaaaa tggccaaatg gcttccgcca accaataaag
     9241 caataaagct tattgtttgc ataacaagtc ccatatacac gatttttctt tcagaataag
     9301 aatctgccag tcgtcccacg ataggtgcag caactgctcc agacaaacca aaaatcgcta
     9361 atatccctgc ttcgaatgtt ccccaattgt aggcgggact gctgatataa aaaatcaacg
     9421 tagaccaaaa gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc gcttctctta
     9481 atagacgttg agaagcaact aaatgcggca aacttttaag tgaatcaagg tatgaaacag
     9541 ctgtactact ttgctggaca gaacgcggca ttttccagta aagcagaagt gtaagaattc
     9601 cgactgcaca taaagcaaat aaatagatca tccgccaaga aaaaagactg gcaacaatac
     9661 cagataccgt ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat gtacccatga
     9721 ccttccctct tgcttttggt cccgctagta ccgcaccgta aggaatgatg atttgcggca
     9781 cgatcgaaag taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac gaaggcgcaa
     9841 aaaaagcggc caacagagag acggcagata acgctgccat tcgaatgatc aattttggac
     9901 gatccaccac gtcccctaaa ggaactaaaa acagtaaccc taacgcatag cctaattggg
     9961 tcaacatcgt caatatcccg actgcacttg tgctaacaga taaactagct gctacttttg
    10021 ttccaatcgg ctggatgtaa tacatattgg caacaacaac gccacaagtg attgataata
    10081 gtaaaataag cgaagttgtt aaaggttttt tttcattact cttcattgat ttcccccatt
    10141 tcctatctta gtttacaact tatggaaaag attggaaata gaaaaataca actattcgcc
    10201 acacttatta tttgtgcttt tgttttcctt gttttttctt gcgatcttta taaatgaaga
    10261 caactaaccc tactagcaca gtaacacttg caaccaacgt gactgcagtt ttgttatatc
    10321 cgtgaaacag agcttctcca ccacacgcat acaacacaga cgaagggatg actccaatca
    10381 cgatcgacaa tatgatttgc tgtagaggca atttcatcgc atctgcagca aaattgacca
    10441 tagaagatgg aataaccggt atcatatagc ctaacattac acctattttt ggatgtttca
    10501 tttgtcggat tgcttgcacc caatgattcg tttcggtttt cttatctaaa aattttaagc
    10561 gatgcataag gaaaattgca gtgatgttac caagagcatt acccaaaatg ttgatgatcg
    10621 cacctgtcag tggtccaaag ctcaaaccag caatgactcc aactactgaa gtaggaatac
    10681 ctggaacagc acacatcacc gctgtcaata cgatcaagag tatcgatgca aaagtcccat
    10741 gcgaacgaat atgtgcgagt agctctgcct tactcgctct cggatttaaa aataattgga
    10801 tgtctgtctg atattctaaa tacaaccgat agcccactac cagtatcaga atgattccca
    10861 acgagatcaa tatcaatcgg attattttct ttttcatatt tcctcctgaa aaaaatctat
    10921 attcattaaa taatagttta acataaaaac aaaaaagcac cgaaatccaa cataggatct
    10981 cagtgctttt aaatagtaaa aattatttaa cagttacaga tgcgccaact tcttcaagtt
    11041 tagctttcaa ttcttcagct tcttctttag atacgccttc tttaactggt gcaggagcgc
    11101 catcaactac agctttagct tctttcaagc ctaaaccagt tgcttcacga actgctttga
    11161 taactttaac tttttggtct ccagctgcag ttaattctac agtgaattct gtttgttctt
    11221 cagcagcagc agcgccacca gcagcagcta cagctacagg agcagcagca gatacgccaa
    11281 attcttcttc aatagcttta actaggtcgt taagttctag gatagttgct tcttttaact
    11341 cagcaacgat gttttcaatg ttcaatgcca ttataatttc ctccgtttaa ttgttctttt
    11401 ttatttaatg ctttatgcgg caattaagct gcatctccat cttctttgtc tgccactgct
    11461 ttgacagcgt aagccacgtt gcggactggc gcttgtagta cagatagcaa catagataga
    11521 agtccttcgc gatttggcaa ttttgccaat gctgtgattt cttccaatga agagacttta
    11581 ccttcgatga taccgccttt gatttctaat gcttttgctt ctttagcaaa ttcgtccatg
    11641 attttcgctg gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg tccagtgaat
    11701 acttcgtcca tgccttcaag gccagctttt ttagctgcac gtgaaagaat agaatttttg
    11761 ataactttca tttctacgtt agcatcacga agttgtttac gtaaacgtgt tacttcatca
    11821 actgttaatc cgcggtagtc aacgactact actgatgcag ctgctgtaaa tttttcgctt
    11881 acttcatcaa cgattgctgc tttttttgcg ataattgctt cactcatttt tgagtttcac
    11941 ctcctgaatt ttgatggaag agttttttgc actgaaaagg caagtcactt ttatagttac
    12001 tttgctcaac cagtattatt catcacctat tcacttcgtt catagtgatt ctactcgctt
    12061 tgctcgaaag gaaactcctt tcgctttgct caaccagtat tgttcatcat ctcttcactt
    12121 cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga
    12181 atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg
    12241 tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta
    12301 tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg
    12361 gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc
    12421 tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc
    12481 atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta
    12541 agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc
    12601 agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt
    12661 agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt
    12721 ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt
    12781 gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac
    12841 agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc
    12901 aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac
    12961 aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt
    13021 agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata
    13081 tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag
    13141 tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg
    13201 cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg
    13261 caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata
    13321 gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa
    13381 taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga
    13441 atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg
    13501 ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata
    13561 cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc
    13621 tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca
    13681 aatttatttg ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc
    13741 taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt
    13801 gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac
    13861 aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt
    13921 ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc
    13981 tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta
    14041 tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg
    14101 cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga aattggcata
    14161 tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc
    14221 tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg
    14281 cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac
    14341 tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat
    14401 actttctgag accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga
    14461 aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact
    14521 caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt
    14581 tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat
    14641 gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga
    14701 aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt
    14761 cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt
    14821 tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa
    14881 tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg
    14941 tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt
    15001 atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga
    15061 agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg
    15121 tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat
    15181 gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa
    15241 tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag
    15301 tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc
    15361 gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc
    15421 ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt
    15481 agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt
    15541 aatccatcaa tttcgtatat tttacttcat cgatcgtttt tgttcctgca agttgcatcg
    15601 tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta
    15661 gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag
    15721 ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt
    15781 tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca tctagttgac
    15841 gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg attactgcat
    15901 cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt
    15961 tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc
    16021 cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg
    16081 gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg
    16141 ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc
    16201 tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg
    16261 cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta
    16321 ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg
    16381 cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt
    16441 cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt
    16501 tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc
    16561 gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat
    16621 tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat
    16681 actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc
    16741 caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag
    16801 gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca
    16861 tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt
    16921 tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa
    16981 tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc
    17041 gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt
    17101 ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg
    17161 gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa
    17221 gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg
    17281 accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt
    17341 cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc
    17401 cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt
    17461 ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt
    17521 atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca
    17581 gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag
    17641 aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc
    17701 gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat
    17761 gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc
    17821 aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg
    17881 caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg
    17941 tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac
    18001 ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg
    18061 aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac
    18121 acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac
    18181 tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat
    18241 tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt
    18301 cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa
    18361 ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt
    18421 cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga
    18481 tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt
    18541 cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg
    18601 ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca
    18661 agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta
    18721 tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt
    18781 gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag
    18841 gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag
    18901 agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc
    18961 cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc
    19021 gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta
    19081 caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc
    19141 cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt
    19201 tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg
    19261 cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca
    19321 gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga
    19381 tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga
    19441 aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa
    19501 gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt
    19561 ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat
    19621 aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac
    19681 taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg
    19741 catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt
    19801 ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat
    19861 gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact
    19921 ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc
    19981 tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt
    20041 cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga
    20101 accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc
    20161 ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg
    20221 atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg
    20281 atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga
    20341 gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa
    20401 ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt
    20461 ttcggtcttc ggaccttttt cattttccta gacttctgct ataataacca agaattaatt
    20521 ttgtaacaga tttttttgaa aaaacaagtt tctttcattc aaaaaataga aaaagcttgc
    20581 taattattgg ttaaccagca aacaggagta ttaaaatatc ttgttttatt gaatagcagg
    20641 aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg
    20701 gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac
    20761 ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa
    20821 cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca
    20881 tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag
    20941 aattcccagt tgcttatatt ccagcaggtt ctggagatga ttttgctcga ggggcaggcc
    21001 ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag
    21061 tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt
    21121 tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc
    21181 gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat
    21241 tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa
    21301 acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc
    21361 caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct
    21421 ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt
    21481 tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag
    21541 aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt
    21601 tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg
    21661 gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct
    21721 ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg
    21781 aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg
    21841 tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg
    21901 gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc
    21961 caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt
    22021 tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag
    22081 tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact aacagaacca
    22141 ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt
    22201 tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc
    22261 gattaaatgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag
    22321 acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac
    22381 ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga
    22441 ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg
    22501 taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc ggcatctttc
    22561 ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa
    22621 aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct
    22681 tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg
    22741 cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg
    22801 tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg
    22861 tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac
    22921 gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca
    22981 gtgtagaaat aaagtttata caaactttcg ctgatcaagt tgacgagatt taattgacca
    23041 acaatcggaa tatgaagatc aatccaatac ttcacttgtt ccgaccccat cataccagca
    23101 aaaaagctca tcgcaatcgt cactgaaatc aagatgctat ccttttgcac cagattcatt
    23161 tttggtatca aattaccaac caatgtacct aacaaaatcg cacacaacga ccccagagcg
    23221 gtaacaatca tcaaccattg ccaacgttca ccaaagtcta cttgatagat aaaacgaaag
    23281 acagctaaca taatataaat ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg
    23341 attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga
    23401 tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt
    23461 ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct
    23521 tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact
    23581 tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc
    23641 atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg
    23701 agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt
    23761 tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa
    23821 ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcgaga
    23881 ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga
    23941 aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta
    24001 taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc
    24061 taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc
    24121 ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg
    24181 gagctgacct tttttataag cgattccatc agaagctatt gttcgctgac aacacgtttt
    24241 ctgggagtaa tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag
    24301 attctttggt tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa
    24361 gaatttctac ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca
    24421 gctccttgat actttctaaa attttattcc gactttgggg gtctacagct actgtcggct
    24481 catctaaaaa aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt
    24541 tcaagccacc actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt
    24601 gaatcacctc ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa
    24661 aataatcgat attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga
    24721 ccccaatcat tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa
    24781 tggttccttt gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac
    24841 tgccgtttgg tcctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat
    24901 tcaatgctac aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga
    24961 aaaactcctt ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag
    25021 tgacgattgg ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa
    25081 tctcggctac tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt
    25141 caaggattcg ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc
    25201 agttatttac tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt
    25261 gcctttttac ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa
    25321 gaaagccacc ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat
    25381 catcccccca tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga
    25441 aaaaaagatc aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat
    25501 tgggaaaaac aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca
    25561 caattagcct tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat
    25621 gtcggtcatc tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa
    25681 gaaccaaaac taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat
    25741 cggattcgtc aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta
    25801 gagtttctga tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga
    25861 ttgaatgaaa cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt
    25921 atcaaacata gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat
    25981 cgcttgcaaa tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt
    26041 ttgaaaacga tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa
    26101 aaccagcaat ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag
    26161 tcattattga gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa
    26221 agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac
    26281 ccatcgtccc gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc
    26341 tgccaaagaa attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa
    26401 agatgaagaa tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca
    26461 gaacttgcaa gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg
    26521 taatgaaatc atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca
    26581 tgcttatgct tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa
    26641 aaacaataaa gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat
    26701 cagccaatta ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta
    26761 tcagcatttg caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc
    26821 attcaggttt ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga
    26881 aagaatcaag gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga
    26941 aaaataggcg ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct
    27001 acatcaggac tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca
    27061 ttgccagatt cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa
    27121 cgcccccctt ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac
    27181 caacgatcat ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa
    27241 tcgtagggag aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact
    27301 tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct
    27361 ccaacaaatg aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca
    27421 ttcgtcccac tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta
    27481 tagttcccat cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg
    27541 gtccggagag tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc
    27601 cggcttgctg attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc
    27661 aacagttcct tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga
    27721 tattgatgga ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt
    27781 gagcaacaat aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt
    27841 gcatcaaact ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata
    27901 cccgtgattt ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg
    27961 aggactttgt ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt
    28021 ttccctggat tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc
    28081 cgatacattg gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt
    28141 tccacaattt tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga
    28201 tctttttgat tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga
    28261 ggaataatac tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt
    28321 tgttcgattt ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct
    28381 ttgtctccta ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc
    28441 agccatgtat cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc
    28501 gtactgactg aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg
    28561 gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt
    28621 agaataggaa aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct
    28681 gcaaaaagat tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt
    28741 aaaataaata gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac
    28801 tgtatgccga atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa
    28861 tggatttctt ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa
    28921 aagtacaaac tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca
    28981 agttggataa ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct
    29041 aaaatacgga tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga
    29101 actgcatgct gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct
    29161 gcaataccga tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct
    29221 tcgactagct ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg
    29281 tagatactga aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga
    29341 tccatcccgt ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa
    29401 ccggagattt gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc
    29461 agcaattcta caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa
    29521 agatacgcta cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta
    29581 agctgccttc cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt
    29641 tcttgaatat cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg
    29701 gtttcactga tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc
    29761 gcggcaactg taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt
    29821 cttctatagt aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac
    29881 tttcagttca tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact
    29941 attgaacaat ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat
    30001 ggctgcagtt aaagaagctc catgtcctac gaataaaagt gggccatctt tatgttgggc
    30061 aaccgcatct gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg
    30121 aaaaggagaa ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata
    30181 tttctggtac atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag
    30241 cttatctgta taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt
    30301 atcacgtgct cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt
    30361 tttcccaaat gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct
    30421 cccttgaaaa cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc
    30481 catacgcagc aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag
    30541 cttgtcattt tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc
    30601 ttcatacact atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt
    30661 catagggatg cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt
    30721 tgtttctttt ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata
    30781 tactttatgg gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa
    30841 agcttctaaa ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg
    30901 ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt
    30961 cgtggctgca cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa
    31021 aacgatatga ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca
    31081 atttgatttt tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac
    31141 gttagtgaag ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa
    31201 aaaacaactt gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa
    31261 aactgctaga aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct
    31321 tcttcaaagg attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag
    31381 gtgacatggc caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac
    31441 gctgacgttc agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat
    31501 aactctgttt ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt
    31561 cttgtggcac caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt
    31621 aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat
    31681 tggtcaaatc agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc
    31741 cttcttttga aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat
    31801 tcaagaagct accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct
    31861 gcgcaatagt ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa
    31921 cgaacattcc ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt
    31981 ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta
    32041 cttcttcttg gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc
    32101 ctcggtcaat tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg
    32161 ctcactttgg caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg
    32221 agaagattag atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat
    32281 tgctagagga gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc
    32341 gagtactggt gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat
    32401 gacgattatt cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat
    32461 gcatactgat ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct
    32521 tactccttca ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg
    32581 gttgcagatc accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc
    32641 aatcgattgg ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga
    32701 caaaactatt gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg
    32761 cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga
    32821 attgaacgta agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa
    32881 acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata
    32941 aaatagccca taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc
    33001 aaagtcgtga taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc
    33061 atcgggatta aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct
    33121 atgacaaacc ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc
    33181 cccgtaacag ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat
    33241 ccatgatctt tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt
    33301 ccagcattac cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca
    33361 cttaccaagt cttcatagtg actgattaaa gaagcggtcg acatacctaa aaacaacaga
    33421 gtgatcagcc aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc
    33481 acattgaccc cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg
    33541 atatcatcga ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct
    33601 aagaagtcat aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact
    33661 gaaagcactc gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg
    33721 tcgcgcagag aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag
    33781 attgtttcag ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga
    33841 ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa
    33901 tggagaagtt ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca
    33961 atttgctttt tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa
    34021 agcatatcag ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg
    34081 tcttcttcaa tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc
    34141 agctgcctgt tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc
    34201 tctaaaaaac tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg
    34261 gcaaatcttt cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt
    34321 caaaccgtct cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact
    34381 cttctgtaaa tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc
    34441 ccagatccat tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa
    34501 aatgaactct tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt
    34561 ctgttagtcg cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca
    34621 ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg
    34681 ataaatctga aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac
    34741 gaagttgcat atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaaaccag
    34801 atgtatcccg atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt
    34861 tataataagc ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag
    34921 acgaaacgcc tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga
    34981 tcggtgtttc atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta
    35041 ccttatcatc cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt
    35101 gaccgccttg aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa
    35161 aatattttac ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc
    35221 tcactaataa aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa
    35281 tggatccgtt cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc
    35341 aattgatcaa ccgttacaag ataatcttct gtattttcca attttatttc tagccattca
    35401 tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca
    35461 atctctgcca actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta
    35521 ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca
    35581 aacagctcgt ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt
    35641 gattcattca gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca
    35701 tccagcaagg gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct
    35761 tccagctcgt aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag
    35821 aaacgcacat cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct
    35881 ccgccgactg agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct
    35941 aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc
    36001 gctactttca tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta
    36061 gacatcatct gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc
    36121 acggatactg gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg
    36181 ttcactcatt tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt
    36241 tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc
    36301 gctgatcagc tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt
    36361 tttatttgtg atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat
    36421 ctcaacaact tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc
    36481 agacatttct tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat
    36541 actatcgact ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc
    36601 acggaactgt ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata
    36661 tggtgctaaa aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt
    36721 cattgtttat tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca
    36781 aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga
    36841 aatcgaatat aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca
    36901 attaacagat aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca
    36961 gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac
    37021 attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga
    37081 agcagaagca tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact
    37141 ttctgatttg aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg
    37201 ggctgaattc cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct
    37261 acacgatttt gaactggaac tagaggtaag gggatgcatc tgtgggaaaa attgctttta
    37321 taaatttttt aaaacgtttt tctttacctt atcgtcctgc taagaacaag atccaacgta
    37381 tgcttgaagc taaaaattag aaacctttca ttttcacaag gcacttgagt tcattttttt
    37441 acttttttct gttaagttat aggatgtgaa agaatctagt cactagttgt atagaatcta
    37501 ttttggggga gattcatatg atcgaaatct atttatttgt taatccttta ggggaatact
    37561 gtttcgaaat ggaacaacag ttattacaat ttatcgaaga agagtacggg acaccatcaa
    37621 aagaaaagat gcaatttcgc tttcttcctt tggtcaattt gcagacaatt ggggatgtca
    37681 tgcaacaaaa aggaatttct cagaacaatc ttgaggaacg taatcactta ttctcaatga
    37741 cttattctgc agcattggac tgcaaagcgg cgcagtttca aggaaagaaa aaaggccgcc
    37801 gttttttgat caaattgcaa gaagcagtcg gctgtaatga cataccatat tctcaagaac
    37861 ttgctgaatc catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa gaagatcgtc
    37921 agtctgactt cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg gaaatgggca
    37981 tcacaaaaca cccttctgca gtcgtttaca attatgcctg cgaccgtgat tttggtgttt
    38041 tagtagaagt aaaaggtaca agtagccttt ggagaatcac agagctgtgt aaaacaacag
    38101 ataactcata tcaagttttt aatggtgaac atttacatac aaatgattct cagttccatt
    38161 caagaactag ccatttacac ttgctttcta attaaaaaaa tacgtcgact cccttacaag
    38221 agagccgacg tatttttttc gtttctattc gagaaaatca aaaatagaaa gccttataaa
    38281 cgatcgatca attcttctaa ttcatttaga cgttgttcaa acatcgacat tgcatcttca
    38341 atatatgcag cctgtgtcat atcaactcct gcttttttca ttacttccac aggataatcg
    38401 ctgttacctg cttttaggta agccaagtag ttctctagtg cctctggttc ctggtttagt
    38461 attttcttcg ccagtgctga agcagcagaa aagccagtcg agtattggaa aacatagtaa
    38521 ttataataaa aatgcggtat ccgtgaccat tcgaatttaa tttctgggtc ttcttcgact
    38581 gctggaccat aatattttgc attcaattta ccataactat cacttaggta ttcactagtc
    38641 aatggcacac ctttttcatc ttccgtatgc atgaaatgct cgaattcagc gaattgtgtc
    38701 tggcggaaaa ctgttccttt aaacccatcc aagtaatggt tgagtacata agcccgtaca
    38761 cgagggtctt tttctgtttc caataaatac tccgttagga tattttcatt cgttgtcgaa
    38821 gcaatttctg ctaaaaagat ggagtagtcg ccgtacacat aaggctggtt cgaacgagtg
    38881 aaataactat gaacactatg tcccatttcg tggacaagcg taaatagctg atccagcgta
    38941 tcatgccaat tcaataaaat atatggattt gtgtcatagc ttcccgaaga ataagcaccg
    39001 cttcgtttcc ctttattttc gacaacatcg atccaacgtt cagagaatgc tttttctacg
    39061 atggccatgt attcttcacc cattggtttc agtgcttcta aagctttttc ttttgcttct
    39121 tcgtacgtaa aggtaattgg agcttcccct aagaccggtg tataaagatc atacatgtgc
    39181 agtttttcca cttctaataa acgtttccgt aattccatgt atcgatgtaa caaaggcaaa
    39241 tgcttgttta ccacgtctac caaagtatcg tatacacttt caggaatatg attattgctc
    39301 aaagacgctt ctctggcaga gctgtaatta cggactttcg ctttaaaatt atgtcctttt
    39361 atatgtgtgc ctaaagtaga agcaaatgta tttctaaatt gttcgtaaac actgtacaat
    39421 cccttaaatg ctgcttcgcg cacccttcga tccgtgcttt ctagcaactg accatacacg
    39481 ccatgagata attggactat ttcaccattt tctccttcaa tcgttggaaa aactagatct
    39541 gcattattca aaacagcaaa tgtatcactt gaagcatcaa agatttcacc tgctccagca
    39601 agaagagatt cctgctcagc agataagaca tgggctcgat tatctactat ttgctggata
    39661 taatggcgat agacttccaa ttttggttct tctttgaaat attgccaaat ctggtcatct
    39721 gacaattgca atatttctgg ttcaaaccag gaaacggctt cactaacttt cgaaaacaga
    39781 ctgcttgctc ttgcataaag cgcttggtaa tctgtatttc cagtgtcttg atcgttttta
    39841 aggtgcgcat atacataaat gacttcagtt tggcgataaa ccctcaatac gaattcaatc
    39901 gcatttaaaa attgagaagc gccttgatca agtgttcctt tgtgtttttc agattgtttt
    39961 aactcttctg ataattccaa atatttctca tcaaactctt ggtcgcttga aaagatcttg
    40021 gtcaagtccc aagttaaatt ttcaggcaat tcttctcgtt ttggcaactg ctttacttcc
    40081 atgaaatcca ctccttttct tcccttagct ctatctatca aaatagatca tagtctaaca
    40141 tccacatgtc acagcatcta acttttctac tcattcatgt gtctgcttcc atcaatttta
    40201 gcataactaa atcttgtttt acatagaaaa ccgattccta gcctattttt atacttacta
    40261 ttttttcaaa gaagacaagt gtatgcattc gttaaaaaac tgctgataga ccatctcttc
    40321 atcaatcata ggaaaaagcc attccctttt atgttctttt atttggcaaa gccatgagtt
    40381 gaaatcagga actttctggt tttccaaggc ttcttcgtac aacattcgat aaagaaaaac
    40441 ctgttcttgt aaataaaaga aaaaccgact tttttgatag atccacgatg atagatgcaa
    40501 aagatgttta tgacgcaagt agcattgttc ttgtatggat actgtattct tttgcttgtg
    40561 aataagttgg cgtgtcagcc atttcttccc atcttcactc actttttcta tttttttcat
    40621 ttggttcatt ttaatttcgt tactagtgaa tatttcttta aggtccctac tagaaaaagt
    40681 ccagcagcta ctgcaaaagg aaatatgccc tgatacagtc tctgtaatat gatgatacaa
    40741 ataaagcttt tgtttcttga gatcagcttt ccagcaatgc actcctcttt ttctattata
    40801 gtagcagcaa cttttttcta tcaatgagaa ttgcgattgt ccaagaaaag aattaccaag
    40861 tatccaccat acttggtaac tgttattcaa ataattttgg gttctttcaa taaatctctg
    40921 gtgagacagt ctagaacatt ggatttctat tgctagattc gccaacaaaa tatccggcct
    40981 ttgccgcaac ctcggcaaat aggcttccaa aaatactggt tctactgact gtcccagcca
    41041 atccatgaag cattccttta actgtaagtg ctcttttgat tctctttcac taaagaccga
    41101 acagttatct gatttttgat gggcaaaatg agcacatttt ttctttccct ttttcaaaat
    41161 cactcttttt cgacagccac gacaataata tttctttcct ttttctgcat gtttagcaaa
    41221 acagcactta ttatattcat tcattgcaag cagcattttt ctccctctga acttatttac
    41281 gtctaagaat ttatttacac gaaaaaagag ggcgtgaaaa agctattctg catcaagtaa
    41341 taagaacagc taaacaaaaa tagttcccaa tatttttcgt ttggttgggc ttgttaccgc
    41401 agatgcaagc ttttgaacac atttatacga aaaaaagact gcgacacaat ttttgtcaca
    41461 gtctcttact ataatgctga tttatacaaa ataaaatttc gttcgctcga tagcattacg
    41521 cgccatcaga cattgtccat attcattcaa tgtttctggt gtacgatcgc tcttttcagc
    41581 aaactccaaa attcgagcaa tctgattctc tacatccgct tggttgagat tttccatcca
    41641 gaagtgaacg actaggtaat agcgctcttc gaataaatac aaatcagtcc aagcagactc
    41701 tagttcgaca tctgttgcaa gttggatcac ttgttcaaaa tcttcaaaag caaaagttgc
    41761 ttgtctttcc tgttcttcta gggcgcttcc agataattct tcctccggat cgctgccaat
    41821 aatctgctga cgaagaaagt cactcacatc ttctgaatca acagtagaat cttctgattg
    41881 agaaatttct tcatccggaa gatttttact gataaacagt tctaatccgt ctccttttgg
    41941 caaaacttgg aaagtgacag cttcgcttcc tcggaactcc tcttcgatat caacttcttc
    42001 caaaatacta taaaagaaat tttcgatttc tttgtgattg ccaagtaaat ccaaaaatga
    42061 aacgccccgt tcctctaaat cttcatgacc aattagtaca cggatcgtat tttcattaat
    42121 atgttccatt tccattttgc tacacctcac tttgccacaa tgtttaatat agattcattg
    42181 tagcgaaatc agcttaaatg taaagaaaaa aaatctcgct gttaagcgaa tttaataaaa
    42241 ccagcggatt tttcaacctt tcaagctatc ttatttaact ataacatttt tggcagacga
    42301 atactatata gccgactttt cagctaattt tccctctcta tttttcgtgc aatcatcatc
    42361 ttctactacc tttttataca tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg
    42421 aaaaactatc tttgtaat
//
LOCUS       DAZKNM010000008        40711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000008 DAZKNM010000000
VERSION     DAZKNM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.008"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..513
                     /gene="zapA"
                     /locus_tag="ACG8M9_000349"
     CDS             76..513
                     /gene="zapA"
                     /locus_tag="ACG8M9_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0377150.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            571..1119
                     /locus_tag="ACG8M9_000350"
     CDS             571..1119
                     /locus_tag="ACG8M9_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0377151.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            1234..3594
                     /locus_tag="ACG8M9_000351"
     CDS             1234..3594
                     /locus_tag="ACG8M9_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0377152.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            3681..3995
                     /gene="trxA"
                     /locus_tag="ACG8M9_000352"
     CDS             3681..3995
                     /gene="trxA"
                     /locus_tag="ACG8M9_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0377153.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            4177..5955
                     /gene="uvrC"
                     /locus_tag="ACG8M9_000353"
     CDS             4177..5955
                     /gene="uvrC"
                     /locus_tag="ACG8M9_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0377154.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            6100..6999
                     /locus_tag="ACG8M9_000354"
     CDS             6100..6999
                     /locus_tag="ACG8M9_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377155.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            6992..8254
                     /locus_tag="ACG8M9_000355"
     CDS             6992..8254
                     /locus_tag="ACG8M9_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377156.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            8415..10313
                     /locus_tag="ACG8M9_000356"
     CDS             8415..10313
                     /locus_tag="ACG8M9_000356"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0377157.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(10643..11362)
                     /locus_tag="ACG8M9_000357"
     CDS             complement(10643..11362)
                     /locus_tag="ACG8M9_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0377158.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    11552..11599
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            11699..12031
                     /locus_tag="ACG8M9_000358"
     CDS             11699..12031
                     /locus_tag="ACG8M9_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377159.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            12018..14021
                     /locus_tag="ACG8M9_000359"
     CDS             12018..14021
                     /locus_tag="ACG8M9_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0377160.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            14040..14510
                     /locus_tag="ACG8M9_000360"
     CDS             14040..14510
                     /locus_tag="ACG8M9_000360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0377161.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            14531..15115
                     /locus_tag="ACG8M9_000361"
     CDS             14531..15115
                     /locus_tag="ACG8M9_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0377162.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            15130..16116
                     /locus_tag="ACG8M9_000362"
     CDS             15130..16116
                     /locus_tag="ACG8M9_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0377163.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            16106..16417
                     /locus_tag="ACG8M9_000363"
     CDS             16106..16417
                     /locus_tag="ACG8M9_000363"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0377164.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            16439..18220
                     /locus_tag="ACG8M9_000364"
     CDS             16439..18220
                     /locus_tag="ACG8M9_000364"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0377165.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            18213..19589
                     /locus_tag="ACG8M9_000365"
     CDS             18213..19589
                     /locus_tag="ACG8M9_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0377166.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            19596..20231
                     /locus_tag="ACG8M9_000366"
     CDS             19596..20231
                     /locus_tag="ACG8M9_000366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0377167.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            20261..21616
                     /locus_tag="ACG8M9_000367"
     CDS             20261..21616
                     /locus_tag="ACG8M9_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0377168.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(21808..23202)
                     /locus_tag="ACG8M9_000368"
     CDS             complement(21808..23202)
                     /locus_tag="ACG8M9_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0377169.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            23294..24265
                     /locus_tag="ACG8M9_000369"
     CDS             23294..24265
                     /locus_tag="ACG8M9_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0377170.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            24262..25044
                     /locus_tag="ACG8M9_000370"
     CDS             24262..25044
                     /locus_tag="ACG8M9_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0377171.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(25213..25584)
                     /locus_tag="ACG8M9_000371"
     CDS             complement(25213..25584)
                     /locus_tag="ACG8M9_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0377172.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            25718..26161
                     /locus_tag="ACG8M9_000372"
     CDS             25718..26161
                     /locus_tag="ACG8M9_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0377173.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(26204..27013)
                     /gene="yidA"
                     /locus_tag="ACG8M9_000373"
     CDS             complement(26204..27013)
                     /gene="yidA"
                     /locus_tag="ACG8M9_000373"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0377174.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(27032..28399)
                     /locus_tag="ACG8M9_000374"
     CDS             complement(27032..28399)
                     /locus_tag="ACG8M9_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0377175.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(28383..29213)
                     /locus_tag="ACG8M9_000375"
     CDS             complement(28383..29213)
                     /locus_tag="ACG8M9_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0377176.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            29376..29813
                     /locus_tag="ACG8M9_000376"
     CDS             29376..29813
                     /locus_tag="ACG8M9_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0377177.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            29889..30509
                     /gene="rpoE"
                     /locus_tag="ACG8M9_000377"
     CDS             29889..30509
                     /gene="rpoE"
                     /locus_tag="ACG8M9_000377"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0377178.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            30698..31204
                     /locus_tag="ACG8M9_000378"
     CDS             30698..31204
                     /locus_tag="ACG8M9_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0377179.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            31221..32120
                     /locus_tag="ACG8M9_000379"
     CDS             31221..32120
                     /locus_tag="ACG8M9_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0377180.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            32207..33091
                     /locus_tag="ACG8M9_000380"
     CDS             32207..33091
                     /locus_tag="ACG8M9_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0377181.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(33283..33354)
                     /locus_tag="ACG8M9_000381"
     tRNA            complement(33283..33354)
                     /locus_tag="ACG8M9_000381"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33319..33321),aa:Arg,seq:ccg)
     gene            33414..34748
                     /gene="rpoN"
                     /locus_tag="ACG8M9_000382"
     CDS             33414..34748
                     /gene="rpoN"
                     /locus_tag="ACG8M9_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0377182.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            34897..35988
                     /locus_tag="ACG8M9_000383"
     CDS             34897..35988
                     /locus_tag="ACG8M9_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0377183.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            36029..37030
                     /gene="gap"
                     /locus_tag="ACG8M9_000384"
     CDS             36029..37030
                     /gene="gap"
                     /locus_tag="ACG8M9_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0377184.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            37140..38330
                     /gene="pgk"
                     /locus_tag="ACG8M9_000385"
     CDS             37140..38330
                     /gene="pgk"
                     /locus_tag="ACG8M9_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0377185.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            38434..39189
                     /gene="tpiA"
                     /locus_tag="ACG8M9_000386"
     CDS             38434..39189
                     /gene="tpiA"
                     /locus_tag="ACG8M9_000386"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0377186.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            39305..40603
                     /gene="eno"
                     /locus_tag="ACG8M9_000387"
     CDS             39305..40603
                     /gene="eno"
                     /locus_tag="ACG8M9_000387"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0377187.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        13534 a         7085 c         8716 g        11376 t
ORIGIN      
        1 tgtggagaac ggtgaaagaa ccaacttcat ggtacactaa ataaaacttt catcaaatag
       61 gaggaaatga ttcgtatggc gcacgaaaaa acacgctaca aagcggtgat tgccaaccaa
      121 acatatacta ttatcggacg ggaaaccaag catcatatgg atatcgtaac gaagctgatc
      181 aatgaacagt tagcagaatt gaaacaatta tcaccacaaa tggataatga acaagcagca
      241 atcttgatgg ctgtcaatgc gctttcggat caattgaaga aacaagaaag aatattggag
      301 ctggaagaag aaacagcaga attaaagaaa aaaatgatca aatttacaga gcttgaaaat
      361 cgtgtgaaac gcattgaagc aatcgagaac gaagctcggg aagtattgaa agaaaatggt
      421 caagcagatc ataccattaa aaatcatgtg gaagcacaac agattttaaa cgagaaacga
      481 aaaggacaaa ttaaacaaaa atcttctaat taagatcctt ttatcaaaat aagaaaaatt
      541 taaaaaagtt agaagagaaa ggaacgtagc atgctgagtt tgctgatttt atttttattg
      601 ctaattgcgt ttttttctgg agcacgtcgc gggtttgctt tgcaaatcat attcgcgatt
      661 ggatatgtat tgtcatttat tgctgcccag catttttaca aaccattagc aagtcatttg
      721 gaattataca ttccataccc agctgtcacc ccaacatcaa agttagcatt ttttgatcag
      781 gtatttgcct ttcacctaga tgaagctttt tatgcaggaa ctgcttttct gattattttg
      841 ctaattggct ggctgcttac ccgatttgtg ggggtttttg tccatggatt gacgtatgtg
      901 ccgatcttga gacaggtaga ctggattgca ggaggcatcc tgtcactaat catggcttac
      961 gttactatat ttttgatttt gcagttactt gcctttgttc cattagatgt ggtccaaaat
     1021 caattcggcg agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac
     1081 aagatgtatg agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata
     1141 tttcgttttt tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag
     1201 ctcttttttc atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg
     1261 gaatttgaaa aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa
     1321 gaactggcag aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa
     1381 actgaggatg gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaaattgaa
     1441 aatatccgtc cccatatgaa acggattgag atcggagcag acttgaatgg agtggagctt
     1501 gcacaagttt ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt
     1561 tctgacagtg aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc
     1621 cctgttttga gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac
     1681 gcttctcctg agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt
     1741 gaacagttag atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc
     1801 accatgcgaa atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc
     1861 ggtgtagtcc acgatcaaag tgcttcaggt cagactttat ttattgaacc aaaacaagtg
     1921 gtggatttga ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt
     1981 attttaagtg aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatact
     2041 tatgtgattg gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa
     2101 gccatcgtgc cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca
     2161 ctgatcgatc aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc
     2221 atcgtcatta caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg
     2281 ttacaactga tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt
     2341 atttttgagg aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg
     2401 accttttctt ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt
     2461 ctggtattat ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca
     2521 atcgcaatct tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat
     2581 cctgaattga aagtttatgg atataatcgg gcaaatacga ttaatgccag catggagttt
     2641 gatgtggata cattaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat
     2701 gcgtttgaaa tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag
     2761 ttgatgaatg atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg
     2821 aaaatggcag agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt
     2881 cacgatgatt tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa
     2941 aaagcaaaga aaaaagcaaa tgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc
     3001 attgctgata ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag
     3061 ttgatcgatg caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat
     3121 aaagtattga agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt
     3181 acgacttacg gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa
     3241 atcggtatat taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa
     3301 gagccgacac aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat
     3361 caattggacc ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg
     3421 gattctgcca ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc
     3481 gcacttcgca agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag
     3541 tttgcaccgg caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta
     3601 ctaaaggtaa gcagatggtt cgctatccaa gtatagaaat gctatactag tcatagattt
     3661 taaaaatgga ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa
     3721 cagataaagg acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc
     3781 aagcaccgat tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta
     3841 aaatggatgt ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa
     3901 cattaatgtt gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag
     3961 aacaactacg tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag
     4021 ggtgggactg aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa
     4081 ttaatatttt ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct
     4141 ataataactt taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa
     4201 ttagctttac ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc
     4261 atttatgtcg gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggtagc
     4321 cataatacga aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca
     4381 gaatcgaata tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa
     4441 tacaacatta tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa
     4501 tatccccgtt tggtgattac tcgtaaggta ctaaaagaca aagcactgta ttttggacca
     4561 tatcccgatg tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg
     4621 agaaaatgca aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgc
     4681 ctgtgtcctt attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg
     4741 aaaaactttt taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa
     4801 aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc
     4861 attgagacaa tcatgacttg ccaaaaaatg acaaatactg atttactgga tcgagatgta
     4921 tttggttatg cgattgataa aggatggatg tgtgtccaag tgtttttcgt aagacaagga
     4981 aagctaattg aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc
     5041 ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg
     5101 attcctgata cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa
     5161 ccaaaacgag gagaaaagaa aaaattagtt gaattggcga gtaagaatgc ctcagttgct
     5221 ttgaatgaac ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa
     5281 cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct
     5341 aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca
     5401 agaaaagaat atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca
     5461 atgagagaag tcatctatcg cagatattcg cgagtgattc gcgaagggtt gcctttacct
     5521 gatttgatct tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa
     5581 aatcaattag gaatcgatat tcctattgct ggcatggtaa aaaacgataa acataaaaca
     5641 aatgagttgc tattcggtac agatttggct gtagtaccgc ttgagcgaaa ttcaccagag
     5701 tttttcttac tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg
     5761 cagctacgca gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca
     5821 aaacgaaaaa aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg
     5881 acagaagaat tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa
     5941 ttacatcagg aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat
     6001 gtacaacaaa aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt
     6061 ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt
     6121 gttaaaactt ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga
     6181 aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc
     6241 ttggattttt tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag
     6301 aaagattatg atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg
     6361 attcaagacc aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa
     6421 ccaaaaatcg acttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta
     6481 aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa
     6541 cctaaattga tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg
     6601 ctaaaagatg gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac
     6661 aatatggata atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg
     6721 gttttgaatg gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg
     6781 gaatctggtt taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt
     6841 catgaagaca atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt
     6901 actcgtgcga ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa
     6961 gagattttca aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga
     7021 tgtctattta aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat
     7081 catgctgggt atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa
     7141 aatcggcgta ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga
     7201 tgattttgag ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga
     7261 aaaaatcgat gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta
     7321 tagcgaaaat tcattaggac aaagtacaca actactgata caacaacaac tgactggctt
     7381 gcaatcgatg cttcgagcga gtcaattagg catttcacca gaagaagtag ctagcttgag
     7441 ccaacaagca agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt
     7501 cggagaagat aactcgacag tccaatacgt cgtaagttat gtagcaacga ttgtcttgtt
     7561 cattattatt ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac
     7621 gcggatcatg gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaatt
     7681 aacaggtgtt ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat
     7741 aggatacagt caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt
     7801 ggataacttg attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt
     7861 gattttttct gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc
     7921 aaaagcaatt ttgccagtga cttatctatc tctaggcgga tacatgcttg gattgatact
     7981 aggagcttca gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc
     8041 ttcatatatt atgccgattc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat
     8101 ttccttgttg gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta
     8161 caaatcgaat gtactcgttt atagcgaagg cggattatgg agttcattga aacaatcgat
     8221 ttcaattatg aggaatgaac gaaaaaaagc ataaaacaat cctaaaatac agaaggaagc
     8281 tggacatcta ttgttcccag cttctttttg tatagaccta ttttatttat tttactatat
     8341 tggatatata ttcattgaaa atagtgtata acaactttat aatattgagt ataccaattt
     8401 ttggaggcgt tttaatggac cgttatttag atttactttc agaaaaattc ccctcaacgg
     8461 aggatgtcat cacggaaatc atcaatctcg aagcaatttt gcagcttcca aaaggaacag
     8521 agttattttt gagtgatata catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg
     8581 gttcaggaaa tcttaaagaa aagatcaaag accttttttc tgaacaaatg acggaaacgg
     8641 atatcagcca attcactatt tttacggctt atccagaata tgcattaacg actgattggt
     8701 acaaagaaca agataaatcg cagctgatcc atcatttgat cgatctttta cggtttacga
     8761 cagtcaaata tacccggtca aaagttcgaa aaggattgcc gaaagaatat agctatatca
     8821 ttgaggaact cttgtatatc gatgaccgta tcaatggaaa aaaagactat gtaaaaaaaa
     8881 tcattgaaca acttgttttg atgaaggaag aagaaaaatt tttaacgaaa cttgcacaga
     8941 cgatccaaaa atctgtcatc gatcatttgc atatcgttgg agatattttt gaccgaggaa
     9001 ctcaagcagc caaagtgatg gatcgaatca tggatttttc ttctgtagat atccaatggg
     9061 gaaatcatga tattttgtgg attggtgcgt attgcggctc tgaggcatgt ttactgacac
     9121 ttttacggat tgcagcacgc tataattatt tatttgaatt agagaaagaa tatgggttga
     9181 atttacgtcc attgttttct tttgctcatc aaacttatca gaagaatcca gtatttgcgc
     9241 ctaaaaacag taaaaatcta tccgaaagag agcaagaaga attagaaaaa atccatcagg
     9301 cactttctat ccttcaattc aaatcagaac atcagttatt ggatcgtcga cctgaattta
     9361 aaatggacga tcgtaaattg ctggaaaaaa tcgactatga agcatcaacg attgatttag
     9421 aaggtcagct ctatgagttg aaaggaactt gttttcaaac gatccagcca gatgatccaa
     9481 aaaaattctt ggaagaggaa caaaaagtga ttgatagttt gatgtattca ttccagcact
     9541 ctttgagaat gcaaaaacat atgacgtttt tgatcgaaaa agggggaatg tatctcacga
     9601 acaatcagca tgtgctgttt catggctgta tacctgtaga cgagaaggga cagctcttag
     9661 catttgaatt agaccaatct tatcgcggaa aagaactgct gggctttttt gaaaaacaga
     9721 taactgaaag cgcaaaagat ctgactgcaa aagaagattt agcaaccgat ttgatttggt
     9781 atgcgtggag tgggccattt tcgcctttat tcggcaagag taaaatggcg acttttgaac
     9841 gttattttct cacagagcct catacgcacg tcgagaaaag caatccttat tatcacttgc
     9901 gagatgaaga gtggttcagt gaaaaaattt tagcagaatt cgacacaaaa gaagaagggt
     9961 cagcaatcat taatgggcac acgccagtaa aagtaaaaaa aggggagtca ccgatcaaag
    10021 caaatggaaa agtatttgtc attgacggag gtttatccaa agcttatcaa aaaacgacag
    10081 gaattgccgg ttattcacta ttatgcaatt cttatggttt ccaaatcgtg acacattatc
    10141 cgtttttgcc tattgaacaa caatttgcaa aaaaatccga ccaaacaaat gtgaaaaaag
    10201 taatcgaaca gcaactacca cgcaaactca atcgtgatac aacaaaaggt atggaactgc
    10261 agcagcagat tacccagctc aaaaggctgt tggactatcg aaaggacgat tgagaagaat
    10321 tccagtgaac attgttccat agactggaga ctgagacaaa agtcgtgtcg cagtctcttt
    10381 ttccggataa acggtgttct tctgccaact cctcggtcac aagtcacact attcaacaaa
    10441 catgaaaagc tgttttttga aattgctcct tgtgactcag agttgaacgg caggctcacg
    10501 acctcttggt ataaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca
    10561 aaaatgtgaa aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt
    10621 cacaaccccc tcagaaagtc tattaaaatt cattcacaaa gaaatcgaat aagcgctgtt
    10681 cagatggatg tacacggtgg gtaagttccg gatgccattg aacaccgagg attttatgca
    10741 tatcatcttt gctttgaacc gcttcaacga gtccatcgtt agaaaaagcg atggctttta
    10801 ataaaggtga aagatctttg actgcctgat gatggtaaga attcacaaaa tatggatcgg
    10861 ggagaaggtt gcttaacaaa ctgtctgaaa caatcttgac ttcgtgagtc gtaaattgcg
    10921 gggcagtggg ctgttgttca tgttttactg accactttgg atataatgaa agatcttgat
    10981 aaagtgtacc ttccaaaacg acattcaata gttggagtcc ccggcagact gcaaaaattg
    11041 gttttctttg tttcaatgct tccaagatca gtgcctgttc aaattggtct cgttgaatat
    11101 ttgtttccgt tagctttgga tgaggatctt ccatgtaaag ttgtggactg acatcttgac
    11161 cacctgcaag aataagtttg tcgatttgtc caatcagttg aggagctgaa tctggagaca
    11221 tgactggcag aataagaggc aatccttgag catctaaaac tgcatccacg aatccttgcg
    11281 gtgtatagct cacttgattt ccttggaaag tatctgttgc tcgaatcagt tgatttccgg
    11341 caatgccgat gattggtttc atttgtattc cccctaaagt agacttattg taacaaggat
    11401 ttaaaaaaaa ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac
    11461 tatctgtaaa gagaaatttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt
    11521 tgttataata aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt
    11581 cttcaagggg tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag
    11641 aggtacatac tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt
    11701 gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgaaaagcg
    11761 tgctcaaaca ctgcggcagc agctgaagat ctatgcaaca gaaaaagagc aggaattgga
    11821 aacgatccaa acaaactatc gacaacaact aaaacagttg gtggaagaaa aagaacaatt
    11881 tgaaatacaa gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca
    11941 agcaactttg aaagatcaat atcaaaagca tcagagtcaa gcgatcggtg cgatcattga
    12001 aagggtgaaa gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata
    12061 aaagaaatca ggagaggatt ttacaagcag tacaaggaat ccaaaacgtt gaaatccggg
    12121 atctgtatca aaatagaaca aacaaccagt gggtggagaa gtattttcca gataccacga
    12181 ttattgataa agaagcaaaa ttaagcgctt taggtaatcg gttgaaagac atccaagaag
    12241 ctatccaatt catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc
    12301 aagaactttc cttacaagat ttggaagcca attattccga agaaaatttc ataaataaat
    12361 tgaaagaagt ccttgattta aaagaaaagt gggaacaact aactgaagaa cgtgatcgtt
    12421 tagaagaaga ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt
    12481 atgcaagtaa gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt
    12541 tcaaggaaga actgagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa
    12601 aacaagttta tctttcgtat gttgctttaa aaaaatcagt tgctgttata gaagaaattg
    12661 ctgggaaata cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaac
    12721 tgcaagagac caagcagcaa ttatccattg ttcacgagca gcaaaagaag ataacgacag
    12781 cgttaggtca gtgcagtggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg
    12841 ccgttgcaga acgagaaaaa ataaaagaac ggtttatcca tgcgacttat ctgatcgttt
    12901 tgcaaggttg ggtagatgca gaagaaaaac agaatttgct gcatgtgtta aacgaaaccg
    12961 tatcagaaga agatatatac gtcagtttcg aaacaccaaa ctcagaagag attcagcagg
    13021 aagtccctac taaattgaaa aaccatccgc ttgtcgaacc ttttgaattg ctgactgaaa
    13081 tgtacagtct gccaaaatat gaagaagtag atccaacacc ttggatgaca ccattttatc
    13141 ttgtcttctt cgggatgatg gtcgcagatg ttggttatgg tttgctgatg ctcattggga
    13201 caatactagc tcaaaaacta cttgttctac cacgcgggat gaaacgtttc gtaaaattct
    13261 ttgagattct ctcgatacca tcgatcatct ggggattgat ctacagttca tttttcggac
    13321 agtcattacc aaaagaactg tttggcatcc gcttgccttt cccaatccta tctacgacgg
    13381 atgacgtcaa tacgattttg attttatccg tgattttcgg tctgatacag attttggttg
    13441 gattatttgt ggcagcaaag gaaaatatca ggagaaaaga ttacttgagt gctgtcagtg
    13501 atggatttgc ctggcaaggg atcctaattg ggatcgttat tgcgttggtt ggtgccatgt
    13561 tgataaaaaa tacagcattt gtttaccttg gcgctgggct atcgatcatt gctgccttat
    13621 gtatcttgat cgttccgatt atccaaacgc cgtctaaagt caaaggtgct gccaaaggtg
    13681 cgtataatct ttacggactg accggctata tcggtgattt agtcagttat acacgtttga
    13741 tggcattggg tatttcaggc ggaagtattg gagcggcatt caatatgcta gttgctttca
    13801 tgccgccagc tgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga
    13861 atatgttttt gacactgcta agtgcctatg tccatggcgc acgattgcag tatgtcgaat
    13921 tttttgggaa gttttataca ggaggcggac gtgcctttga tccgctgaaa acggcggaaa
    13981 aatacgtcaa catcaatcac aagaaaaaaa acaataaata attggaggat ttttatataa
    14041 tgatggatta cttaattagt caaaatggtg gaatggtatt tgcggtatta gcgatggcaa
    14101 cagcgacgat tttttcaggg atcggttcag ctaaaggtgt tggaatgaca ggtgaggcag
    14161 cagcagcatt gacaacaagt caacccgaaa aattcgggca ggctctgatt ctgcaattgc
    14221 ttccaggcac gcaaggtttg tatggattcg ttattgcctt tttgattttt atcaacttag
    14281 gaaatgacat gtcagttgta caaggactga actttctagg tgcttctctg ccgattgctt
    14341 ttacaggctt gttttcaggt attgcccaag gaaaagtcgc agcggcaggt attcaaattt
    14401 tagcgaaaaa acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat
    14461 acgcgatttt aggtttcgtt atttccttcc tattagtgtt gaatgcctaa gcataaagga
    14521 ggaaaagcag atgaacgcca ttgaaaaaat catctctcag atgaacgaag cagcagagca
    14581 ggaaagagcg gcgttagaac aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa
    14641 acgtacgcaa gttgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattactcga
    14701 aaaaaaatac cgtcagctaa ggaatcgcca gcaagtagaa gtacgacaag aaaatttgaa
    14761 tgcgaagcag gaatttttaa gacgattgtt tgcagatgcg gtaactgaaa tggaaaattg
    14821 ggatgaatct gaacaaatcc agtttatcaa aaatgcattg tattcattac ctttgacagg
    14881 aaaagtagca ttcattgctg gagaaaaatc agcagcctat ctttctcaga cgttgttgga
    14941 tgaatggaac aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca
    15001 agcgggtttt ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt
    15061 acaagatatc caaggaacga tgagttttga gattgccaat caattattcg aataaggagg
    15121 gatcagtgta tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt
    15181 ttgtccaatg agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa
    15241 atattgaaat ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa
    15301 cagaatcttt taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag
    15361 cctgaaatcg tttggatcta tacgatgcgt tatactttcc ataatctgaa ggttttgaca
    15421 aaagctgaga tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca
    15481 ttagaaacct tgaaggatgc cattcatacg caagtttcaa atgaacttcc tgaaagcatt
    15541 atggacagta tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt
    15601 gacgtgattt atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga
    15661 tatcaagaat tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg
    15721 gctcgaggaa tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg
    15781 ggaagtatcg aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg
    15841 aattttctat tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat
    15901 attgatctcg taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa
    15961 gcacaaaccc aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta
    16021 gaaagcaaaa accttcgttt actggttatc ggcaaaagaa gccatttcga atcagaaaca
    16081 atcagaaagc gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga
    16141 ttctgttttg ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac
    16201 cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga
    16261 gcaatgtgca gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc
    16321 tatcgtattg attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa
    16381 aaacgtagaa aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt
    16441 gcaaattgga aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga
    16501 tgccagtatc caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga
    16561 gatgcgtggc gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg
    16621 cgaaccggtt cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc
    16681 acaaatgttt gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa
    16741 tttcttaggg cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga
    16801 accaacagta gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga
    16861 aacaaaagtt ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac
    16921 aatcgaaaaa ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg
    16981 tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga
    17041 aaaattaaac ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc
    17101 agtgacaaaa ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt
    17161 tcagcatcaa attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga
    17221 gcgtggaaat gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac
    17281 aggagaatcg ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc
    17341 agctcgagaa gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg
    17401 ttatgacgtt gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat
    17461 gagcggacgt ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg
    17521 gcttgcggaa tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga
    17581 aggaagtata accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt
    17641 cacacaaaat acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca
    17701 aaaaagacat tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt
    17761 agggaagtat atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat
    17821 gcgtattttg caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc
    17881 cctttcagat aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt
    17941 gcagcaaaat gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat
    18001 gttgaagatg atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta
    18061 tttaagtgag atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata
    18121 tatcccggaa acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat
    18181 gcaaacgatc gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg
    18241 aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa
    18301 tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag
    18361 ataaagcaat ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg
    18421 tacgattttt aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat
    18481 tcgatggttt gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg
    18541 atattaacgg agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa
    18601 caggtatctc tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat
    18661 tttctggctc tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg
    18721 tactcgattc atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag
    18781 aagctgaatt tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt
    18841 ttatgaattt agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga
    18901 ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca
    18961 tgaccaacta tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa
    19021 gaagaggata tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa
    19081 gaatccgcgg actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg
    19141 ataaaactca tccaatccct gacttgactg gttatattac agaaggacag atcatcttga
    19201 ctagagagct ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc
    19261 ggttaaaaga taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga
    19321 atcagctttt tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag
    19381 gtgaatctgc tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa
    19441 aagaatatgt caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag
    19501 ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc
    19561 tggacaaata tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga
    19621 cccgcatgga attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat
    19681 tattaaaaga taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca
    19741 accagctgcg tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg
    19801 caaatgcttc gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg
    19861 ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg
    19921 aatatgatga acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg
    19981 aactggatcg gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag
    20041 aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca
    20101 atgcattaga atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga
    20161 agctggaaga aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta
    20221 ccactgacta agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca
    20281 aataaagaaa cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt
    20341 agttggcggc ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt
    20401 tatcgatgcg cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa
    20461 tacagcagag cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg
    20521 cggtcttggt tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag
    20581 tctcagcaca cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat
    20641 gaagctcatg ctttacatcc taaaattggc ctttgtgatc caattgattg gtacggttgc
    20701 tttatctttt gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt
    20761 ccatgcaatc tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa
    20821 tcaccagcaa aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt
    20881 aggcttcatc gtatggcgtg atttactgga atatcatcag aaaaagaaga tgacgattca
    20941 ttcaaaaatc gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt
    21001 tttcacagag cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa
    21061 cacctttttt atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca
    21121 tatgagccat gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg
    21181 gtcgacggcc ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc
    21241 gatgttgaaa ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt
    21301 gttgcgggca cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat
    21361 tttatcggtg actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt
    21421 cttctctgcg tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat
    21481 agggaaattg atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt
    21541 cttctctctg atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag
    21601 tgtcatgatt ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa
    21661 ataaaataat ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg
    21721 aggcactacc cgttgttgaa cctcgggtag tgcctctttt ttattgaaag gaatcaataa
    21781 gatttgattc ctttatttat caataggtta taattcatat ttttttattt tttcttttac
    21841 gatacttagt ccagaagcta aaatttcaag gttattagag gatagggaaa ttcgaacgaa
    21901 tgcatctggt tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga taccgttagc
    21961 aagaaagtca ttctcaatta tcttagggtc aatctcagga tcaactggaa gaattcgata
    22021 gtaagggaca cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa gctgattggc
    22081 tctttttgta agacttaatt tagtttctaa gatcctattt gcttcatttg aatggaggat
    22141 ttgagtgatg acttccacat ctaatccaga agttttcaca tttatattaa aaattgcttg
    22201 ttctatcttg ctttggacat gtactggaac tacaagataa gcaactcgca atccagaaca
    22261 aataaatttt gtcattccag ctaagtagat tgtttggtta ggtaataaat tttggaaagg
    22321 aggaatgatc gtatgttgat agtaagttgt taaaaatgaa tggatatcat cttcaataac
    22381 ccatagacct tctttttcaa tcacttcggc taattgctct cgtcttttgt gagagatttg
    22441 aaaccctata ggattattac aagaaggcat aagaaagaca ccatgaatct tcttttttct
    22501 acattcctga atcaatatat caggtcgcat tccttgctcg tcaaaatcaa ttggaacgat
    22561 ctctaaatga tataactgag ccaattcaat gaaattagcg tatgtatatc ggtctgttgc
    22621 tattctatca cctggagaga aaagtgcaga aagtgtgata gccaatccat tttgaacacc
    22681 ggaaacaata gatagagatt ctaaggacgg aatgactcct tgagttttta accaatttgc
    22741 tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg taattaagca aaccaacaag
    22801 atttgggtta ttactagttg attgaataaa cgggacaacg aatttattac attcttcaaa
    22861 agaactaacc agaccaaaat caatgatatg ttcggttact tcattggtag agatggtgat
    22921 ggactcaaag acattgggag aaataaatgt gccacttcca atattcgtat acagcaaacc
    22981 tttctcaatt ccatatttat aagccttgcc aacggtagta aaatttaaat ctaaataatc
    23041 agctagctct ctttgggagg gtaactgagt gtttttctgt aatttattct ttaaaatatc
    23101 ttgttctaat tgttgaatca acgatcgata ataaggacgc ttcaattttt gtttgtctgg
    23161 tcgccaagtt aacgggtaat tgtcataaga attaataggc attttctcca ctccttaaaa
    23221 ttgtatacca tacaatttta ccattgattg tattgatttt agaagttaat cttttgtgag
    23281 gagggaattg tctatgaaaa ttattaattt tgaggaagta gtagcttctt ttagttttga
    23341 agaagcgatt caagtaatga aaaaatgttt ttttgattat gaaagtggta aaatcagcca
    23401 aaatcctcga ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt ttgcgatgat
    23461 gcctgcttat ttgggggaga acagatattt tggcacaaaa gtcattaccg cttttccgga
    23521 taatcatcag tatcatttac cttctcattt aggagagatt ttcttgtttg atgctcaaaa
    23581 cggactaccc aaagcattga taaacgcaaa tgcagtcacc tggattcgaa cagctgctgt
    23641 atctgcgctg gcaacagatt acttagcaaa tccgcatgca gaaacacttg ctttaattgg
    23701 agctggtcaa caagcattgt cgcatttgac tgcgatttct accattcgtc ccatcactac
    23761 agtgtttgtt tacgatattt tagaagaaag acgaaatact tttatcgcta aagctaaaaa
    23821 acagtatcct aaaataacgt ttataaatgc aatatcagta gaagatgcag tacgtccagc
    23881 ggaaatcatt tgtacgttga cctctagtaa agaggcattt cttcaaaaag agtgggtaag
    23941 ggaagacgca catatcaatg ctgtgggcac atttactcct gatacaagag agataagtac
    24001 atcgttgatg gaaacttctc aaatctatgt agatgattac tcagctgcct taacagaaag
    24061 cggggacctg cttatagcca tggcagaagg agatttagag gaatcttgta ttgttggtac
    24121 attaggcgaa ttagtttctg agaaaaaaac attttataaa ggagaatcga ggccgacgat
    24181 tttcgacgca gttggtctag ctattgaaga tctttgttgt gcagaatata tttataacaa
    24241 attagagaaa ggggctttaa aatgaactta gattattacg tagttgatgc atttgcagat
    24301 gaggtgttca aaggaaatcc agcagcggtt tatgtgctag aagaatggct gccagaaggt
    24361 acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa cagcatttac agtgaagaaa
    24421 aaccaagaat ttgagcttag atggtttacg ccagatcgag aaatcgatct ttgtggtcac
    24481 gcgacattag caacagcatt tgttttattt aactattaca aaatacctga tgaaacgatt
    24541 aaattttcta ctcaaagcgg taatctattt gtgacaagaa ttaaagatta ttattacatg
    24601 gattttccta gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga agaagcgatt
    24661 ggggctaaga ttaaagaagc ctacttagca cgtgatttat ttttcgtatt agaagatgaa
    24721 aaaacggtgg ctaaattgca gccagatttt acagctatca aaaattttga attaggaata
    24781 ggagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac cttctttcca
    24841 aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct tattccttat
    24901 tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc aaggggcggg
    24961 gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc agttttgttt
    25021 tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa ttggtggaga
    25081 gtggcaacct tatagctaca atggttgtta aaaaaagacg caaacttttt caatttaatg
    25141 aaaagtttgc gtctcatttt aattattggt gtttgttttt tattcaatca agagtaaaat
    25201 gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt caatgacaaa
    25261 gtagtaagaa ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa gaccagcagc
    25321 tttatgttgc tcgtgtaatt tttctagatc atcagctgct atagcgatat gaccatagcc
    25381 gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt catagtcatc
    25441 cccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac ggcggcggct
    25501 ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat ctttcactcg
    25561 aacacatgtg tgagccattt tcatagattc atctctcctt ttccttttac tcaatcataa
    25621 caaaaagaag taaggaaata gttgatttgt gcttgttcat gagaaatttc tcatgtaaaa
    25681 tggatagaaa ataaaactgt taaggtggaa actattcatg gataaaccaa tttttggtga
    25741 aaaaaagcaa tttgctgatt atcaaacccg ttatgcagca tatattgtca tagaaagaga
    25801 tcagcagatt gcagtaatcg aggctccaaa tggtgctttt tttcttcctg gcggggaaat
    25861 tgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag aaatgggaat
    25921 tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact cgaattatcg
    25981 tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga agcgtatagg
    26041 agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag cgcttgtgaa
    26101 attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa ataaaaactg
    26161 aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt acatattttt
    26221 tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt gctgcttctt
    26281 tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca atcatggata
    26341 agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca aggtgctgtg
    26401 cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg aagtaatagg
    26461 gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc gcaattgctg
    26521 catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg tctggtgtca
    26581 tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg tggatcgtat
    26641 aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa tggagatgac
    26701 tgccgacacg gcgtgccatt acttcgatct ctaggaaatc ttcatgtgtc agtccgtgac
    26761 gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa gtaataacgt
    26821 aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct ggtaagggac
    26881 ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc gcttctttta
    26941 cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg atagcgacta
    27001 gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat gcgccattac
    27061 ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga tcacgcaagg
    27121 acggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct tgtcccgcta
    27181 atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca tttcgcaaaa
    27241 tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa ggtagatcgt
    27301 agctagaatt cacagcagtg taataaacag gattatatcc gaccttttct actaacaaag
    27361 tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat ttcagtggtt
    27421 tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat tcagaccatt
    27481 gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct aaagtaaagc
    27541 ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg gcatcttctt
    27601 gatacaactc gtgtgcgcga tgaagcagat gctccaaaat tacttccata ccacgggaaa
    27661 caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa tcttccactg
    27721 catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc acacgtaata
    27781 ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc cgaagcaaat
    27841 agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt gggttggggt
    27901 aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact cggttaagaa
    27961 tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct tcatgattcg
    28021 tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca tgcaacagtg
    28081 ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct attttttctt
    28141 ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa acgcctaatg
    28201 aatgagtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg cctaattgtt
    28261 taatgcgtcg tagacgttgg acttctttcg aattgatcaa atctaagatt acttgatgct
    28321 ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc agcatttgat
    28381 atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc cattctctgg
    28441 atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt agaatcctgt
    28501 aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt gacttctttt
    28561 acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg atccactgca
    28621 ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg cttcatgact
    28681 tgcccacgtt tttcctgatt accgttgaca cttaagtaca tcataacaga aatcccattt
    28741 ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa atcataagtt
    28801 ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg aaaagcgtat
    28861 ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt tgtgtcactt
    28921 tgggggaaaa tcaaagaaat attcaaaatt ccttcatctg atacgatccc taatccacct
    28981 gaatttctga tcactggagc gtaacctgca tcttccagtg tttggatccc gtcacttaga
    29041 aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg ccagaaatgt
    29101 aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa tgcaaaaggg
    29161 gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt cattcaaccg
    29221 gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa agagttgttt
    29281 tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa gaaactagga
    29341 acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa ggaacaccga
    29401 tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat ttttattttg
    29461 atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat aaagagattc
    29521 aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat ggtcatgtac
    29581 agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa gaacggttag
    29641 aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg aaagaactgc
    29701 atgttagttt gaaagaccgt cccacagcag ggaaggttcg aatcgattat gatctgttca
    29761 tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg taaatataaa
    29821 agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg tgaaaggacg
    29881 tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta tcaatgatcg
    29941 aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct gatttagcta
    30001 accagatcca aaactatctt gggaaatcag acagtgaaat tcgtgattca ctcgcacaat
    30061 tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat cgttggggct
    30121 tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga atggacgaag
    30181 aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt atcaatgatg
    30241 atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg acagaagaag
    30301 atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct gcttataatt
    30361 ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac ttgccaggaa
    30421 atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat tatgaagagg
    30481 aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg ttgtgacaga
    30541 gacgatcagc ctatcagaat aaggaggtgt gacacgattt ttgtcacacc ttcttttttg
    30601 tatataaaaa tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt tagcgcacct
    30661 tgtacgtcat attaagtgag gcagaaagga gtggagaatg atttttactg gatataagaa
    30721 aaaacggcag atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa ctgattatca
    30781 aaaaatgttc cgtatcacga tgaattacgt acatgataaa gaagaggcct tagatgtgat
    30841 gcaagatagt tttcataaag ccctgaacta ttttgaaaaa gaaaaagaga tcgagcattt
    30901 tagtgcatgg ttttatcgga tattgatccg cacggcatta gatagttggc gcagacaaaa
    30961 aaggacgcct gtggacttat ttgaaattca agatatcccc caacagttac ctttagaaac
    31021 ttccgttgca gagctccata tgattttaga taaaatagac agtccggaaa aagagattct
    31081 gatcctctac ttttttgaag gatttcgttt gaaagaaatt gcgactatct tggatatgaa
    31141 tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat gctttacgtc aaatcctaga
    31201 atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa ctaaagaaaa
    31261 cttataaaga acaagcaatt cccaaagggc ttaaagagga gatccgccag cgttttatta
    31321 tcgaagagca atcatttata aaagcaaagc ggagaaagag acgtctccaa gggttcggag
    31381 tatctttggc tatcgtaatg attacaacaa gtagccttct ttttaatagt caagttcgaa
    31441 gctttgcaga agatttaccc atcttaggat cagtcattga attgatctta ggcgaacgct
    31501 ttactgatcg atctgaaaaa atcgatattc aggtaccgaa gatcaataca caaaatgaga
    31561 aagaaaataa aacaattcat ggattaaatc aaaaatactt tcgcgaagga caagcagatt
    31621 ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa gttttgggag
    31681 attatcagaa aatcgtagac gataaccggt ttctcgtgat tgaaaggcag atcacccaaa
    31741 cagcagcaga tagccatgtt gaaaaaagat acgacacaat cgacaagaaa aacagtgtcc
    31801 agctctcact tccactttta ttcaaagatg acacctatct atctgttttg acaaaagaag
    31861 tcaaacggca gatggccgaa caagtcaagg aagatccatc taagtactat tggactgagc
    31921 aagatattca agaagggacg atagaaaaac caacacttgt tacgcctaca cgaagttttt
    31981 atttgaacaa agagcatcaa ttagttttga ctttttctca atacgaaatt gctcctggtt
    32041 atatgggtac accagaattt gtcattccta agtccgtgac aaaaacgatt ttggcatcgg
    32101 aagattattt ggatcattag acgagaagta gttgcgacta gcagtttcaa ttttcaagta
    32161 agtacaagta aataataaga agagaaaaag agaaagaaga gatatagtga aaagaaatta
    32221 tttagttatc agtattattg ctttatccgt gcttttgttt ttcgaaatta cttcggtggc
    32281 ctccttgatc accgaagtaa aaggacaaac acactcttcg gagcattcgt cacaatcttt
    32341 caaacaatca gaacaatcaa ccattaaaag ccaatcaact gaaacagcta acgtcaaaga
    32401 agccattcaa gaatcagcgg aaaaggtaca aagtcagtca tcacaatcaa cacaagacag
    32461 tacaaaaacg agccgagatc cggctgtcaa aagcgtcgca atcagctttg atgatggacc
    32521 aggagcgacc acaacaccgc agcttctgcg aatcttaaaa gaaaaaaacg tgcatgccac
    32581 ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa atcgtgaagc agacagctga
    32641 agcagggcac gagattggca atcacacata cgatcatcag aatctggcca ctctttctgc
    32701 ccagtcgatg acggaggaag tgacgaaagc agacactgag atcaaaaaag tcactggcaa
    32761 aacccccact tttgttcgtc cgccctatgg ttctgtcacg aatgtaggag caacgatcat
    32821 ccagcgaccg atcatcgaat ggtctgttga ttcagaagat tggaagacaa gaaatcctga
    32881 tttgattttg cagaagatcc aagcaacagt atacgatggg gcaattattt tattccatga
    32941 tatctatcct gaaacgattc gtgccgttcc tcaagtgatc gattatctga aagagcaagg
    33001 gtatcgcatc acgacagttg gggatttatt agggcatcca acggctgtcg agaactatta
    33061 tggcagaaac gatcatcggc cagttcagta agtgagacaa gtatctttaa taaataacac
    33121 attttaataa gtagttgaac acataactac cttacttgca aaggcttaac aatatctata
    33181 caggtacaac aaaaaactgt ccaccaattt tctttggcgg acagttttca cttaaaaata
    33241 aagaagcaac tattcgtaaa ttatgctaaa atagcagtag aacgcgtcct gagggaatcg
    33301 aacccccgtc tcaagaaccg gaatcttacg tgatatccac tacactaagg acgcaagcac
    33361 tgggataatt gtaactaaac agataaaaaa ttacaagggg attggacaaa aaaatgaaat
    33421 ttgagcaaaa tttttcacaa aaacagcagc agacacagaa actagccatg acgcaacagt
    33481 tgcaacagtc catccaagtt ttacaatata acagtgagga gctgttggca ttcgttgaga
    33541 atcaagcaat ggaaaatcca ttggtggaag ttgttgagcc ggaatggcag ccagattaca
    33601 taaaagcttc ttcatcttct tatgaaggag aagagacaaa ttacctaaat cagattcctg
    33661 atacaaaagg gtctttattt gattcattga tcgaacaagt ccatttgaat taccgagata
    33721 cttttttgag gaaaatcgtt ctttatcttg tagaatacat cgatttgaac ggatttttga
    33781 cgattagttt agaagaagcc ggaaaagaga ttggagctac gcctatccaa atgctcgatg
    33841 cgttgactct gattcagcaa ttagaaccag ctggagtagg tgcacgtagt ttgcaagaat
    33901 gtctcatgct tcaaacagaa cgagatgatt atgcgccgga attagcttat atcgtgttgg
    33961 aagaatgttt tgaggaactg gtggagcgaa aatggaaaga aatcgcacaa cgtttttctg
    34021 ttgatctaca tgctgttcag cagatattcg actatttaca aacgttgagt ccaagccctg
    34081 gccggatttt cgaccgtagc agcgaattgt acattattcc tgatgtccgg gtattagtgg
    34141 acgacgataa aaatgtacaa gttatctcaa accgaaaaaa tcagccaaat atacgctttc
    34201 aagagagtta cttcaaacaa atgtcacaac aagcagataa agaaactgaa aattatttaa
    34261 aagaacggaa acaggaattc gaatggttga agaaaacgat cctacaacgt ggggatacaa
    34321 ttttgcgggt tgctcaagtc attgtttccc ggcaaaaagc tttctttatc gacaaggaac
    34381 gtccaatcaa gcctcttacg ttaaaggaag ttgcaactga gatagatgtg catgaatcca
    34441 cggtcagtcg tgcagtcaac gggaaatatt tggaaacaga cttcggtgta tttgaattaa
    34501 aaaaattttt cactacacga attcctacga atagtacaga acagacagaa gacctttcag
    34561 cagatacagc gaagaaaaag ctgcaggaat tagtcgatca agaagacaaa aacaagccgt
    34621 tgtctgacca gaaattggtt gaattgctga aaaaagatga gatagcgatc tcaagacgaa
    34681 cagtagcaaa atatcgtgat ttattaggaa tcccgagttc atcaaaaaga aaacgttatg
    34741 ataactaatc agtcaaaaga ctagaaaaag tatttcactt tttctagtct ttttagttga
    34801 aaaacgagag caaaactgtt agacttacag tcgtgaggtt gagatttccc tttattgatg
    34861 tgaaattcga atagttaatt tcttttttta tcgtatatgg gtcgttttaa gtctacattg
    34921 gacgtaaagt gtccaactaa ggagatcgct atgctggatg aaataaaaat gattgaatct
    34981 gttgcaccag atattatcga agtgttgcaa gaacgctata aaattttacg aaatatctat
    35041 tggatgcagc cgattggacg tcgaagttta tcggaaagta tggggatcac agaacgagta
    35101 ttgcgaacag aaacagatct tttgaaaaat ctgaatttga tcgacacctc aaaaagcggt
    35161 atgacgctga caactaaggg cgaagaagtt tatcaaagcc ttgaaaactt catggatcag
    35221 cttttaggta cgcatcaaac cgaacaacag ttggcagaat attttggtaa ccaacgctgt
    35281 atcgttgttt caggaaacag cgaagagcag accaaagtgg ctgatgcatt cggcgaagcg
    35341 ctgagcgaag ccctagaccg actacttcca gaaggcgaga atatcatcgc tgtgatggga
    35401 ggaactacta tggctgtggt agccgaacaa ttgtctaatc tggaaaataa gaaacggcat
    35461 aacctgtttg tccctgcaag aggcgggatt ggtgaagcga taactgtaca ggcaaattct
    35521 gtcagtgcaa gaatggctgc taaagcaaac ggtaaccacc gtgcacttta tgttccggaa
    35581 caattaagtc tggcaactta caattcttta ctcaatgagc catctatcca agaagtctta
    35641 aacttgatta gtgaagcgaa ttgtgttatt catagtattg ggcgtgcatt acacatggca
    35701 gcacgtcgta agatgactga aaaagaatta gtcatgctga aacaagcaaa tgctgttgcg
    35761 gaatcttttg gctacttttt caatgaaaaa ggtgaagtcg tttataaggt ccctagaatc
    35821 ggcatagaat tgcaagattt ggaaaaagtc ccgatcatca tggcaatcgc gggcggaaaa
    35881 tcaaaagcta aagcaattcg agcttatatg aaaaatgcac caaaacaaac gtggctcatc
    35941 actgatgaag ctgctgcaaa tgagatttta aaaggggtaa ccctttaaaa taaatatttt
    36001 tttgattttc ataaggagga aatcttaaat gacagttaaa gtaggtatta atggttttgg
    36061 acgtattgga cgtctagcat tccgtcgtat ccaagatgta gatggaatcg aagtagtagc
    36121 aatcaacgac ttgacagatg ctaaaatgtt ggcacacttg ttaaaatacg acacaactca
    36181 aggacgtttc aacggaacag ttgaagttca tgaaggttca ttcaacgtaa acggaaaaga
    36241 agttaaagta ttagctaacc gcaacccaga agaattacca tggggcgaac taggcgtaga
    36301 tatcgttcta gaatgtactg gtttcttcac ttctaaatca gcagctgaaa aacatttaac
    36361 agctggtgct aaacgcgttg ttatctctgc tcctggcgga aacgatgttc caacaatcgt
    36421 ttataacaca aaccacgaaa cattaactgg taaagaaaca gttatctcag gtgcttcatg
    36481 tactactaac tgtttagctc ctatggctaa aacattgaac gacaaatttg gtgttgttga
    36541 aggattaatg acaactatcc acgcttacac aggtgaccaa atgactctag acggacctca
    36601 tcctaaaggt gacttccgcc gtgcacgcgc tgctgctgca aacatcgttc ctaactcaac
    36661 aggtgctgct aaagctatcg gtttagtaat tcctgaattg aacggtaaat tagacggagc
    36721 tgctcaacgt gttcctgtac caacaggttc attaacagaa ttagtaacag ttcttgaaaa
    36781 agaagtaact gttgacgaaa tcaatgcagc aatgaaagaa gcttcaaacg aatcttacgg
    36841 atacaacaca gacgaaatcg tttcttcaga tatcgttggt atgacttacg gttcattatt
    36901 tgatgctaca caaactaaag taatgacagt tggcgacaaa caattagtta aaactgttgc
    36961 ttggtacgac aacgaaatgt catacactgc acaattagta cgtactttag aatacttcgc
    37021 taacttataa gatagacaaa cgtacaagtg taaattatag acatgtacat ttaaaagcgg
    37081 ggaagcaatc gcgcttcttc cgcttttttt gataataagt ttattcagga ggaaataaaa
    37141 tggctaagaa aacagtgaaa gatatcgatt taaaagacaa aaaagttctt gtccgtgttg
    37201 acttcaacgt tcctttgaaa gacggcgtga tcactgacga cactcgtatc aaagcagcat
    37261 tgccaacaat caactacgtt ctagaacaag gcggaaaagc aatccttttc tctcaccttg
    37321 gacgtgtaaa aacagaagaa gataaagaag gaaaatcttt agctccagtt gcaaaacgtc
    37381 taggcgaatt attaggtaaa gaagtaacat tcgtacctga aactcgcgga gaacaattag
    37441 aagaagcaat ccgcaacatg aatgacggcg acgttgtcgt attcgaaaat acacgatttg
    37501 aagatattga cggtaaaaaa gaaagcaaaa atgatacaga actaggtaaa tactgggctt
    37561 cattaggcga tgtatttgtt aacgatgctt tcggtacagc tcaccgtgca catgcttcta
    37621 acgtaggtat cgcttcaact ggtatcccaa cagttgctgg attcttgatg gaaaaagaaa
    37681 tcaagttcat cggtgaagca gtagaagaac caaaacgacc aatgattgca atccttggtg
    37741 gcgcaaaagt ttctgataaa atcggcgtaa tcgaaaactt gattcctaaa gcagataaaa
    37801 tcttgatcgg tggcggaatg acttacacat tctacgaagc aaaaggaatc aagatcggta
    37861 actctctagt agaagctgat aaagtggaac tagcaaaaga attaatcgaa aaagcaggcg
    37921 acaaacttgt cttgccaatc gacaatgtct gtgcaccaga attttcaaat gatgttgaaa
    37981 cacaagtaat cgaaggagac attcctgatg gtttgatggc tttagacatc ggcccagcat
    38041 cagtaaaatt atttgctgat acattacaag gtgcaaaaac agttgtttgg aacggaccta
    38101 tgggtgtatt tgaaatgagt aactttgcta aaggtacaat cggtgtttgt gaagcaatcg
    38161 ctaacttaga aggcgcaaca acaatcatcg gtggcggaga ctcagctgca gcagcagaac
    38221 aattaggatt tgctgacaaa ttcacacaca tctcaacagg tggtggcgca agcttggaat
    38281 tgcttgaagg taaagaatta ccaggactag cagcaatcaa cgataaataa acgaccaagt
    38341 ttgctcaatt ctgacgagta atcgatcaaa aggtgaaggg agttttcatg actccctagc
    38401 catttttcaa taaattaagg agtgtgttcc tcaatgcgta aacctattat cgcaggtaac
    38461 tggaaaatga acaaaactgc atcagaagca aaagaatttg ctgaagcagt aaaaaacaaa
    38521 atcccttcaa atgacaaagt tgattcagtc atcggatctc ctgcattatt cttacaagaa
    38581 ttaatgacat cagctgaagg tacagaacta aaaatctcag cacaaaactg ctactgggaa
    38641 aactcaggtg cgttcactgg tgaaacttca ccagcagctc ttgctgatct aggtgttcat
    38701 tatgtgatca tcggacattc agaacgtcgt gaatatttcc atgaaacaga cgaagacatc
    38761 aataaaaaag caaaagctat ctttgcaaat ggtatgacac caatcctatg ctgcggcgaa
    38821 tctttagaaa cctatgaagc aggcaaaact gctgaatgga tcgaaggaca aattacagct
    38881 ggtttagctg atctttcagc tgaacaagtg tcaaacatgg ttattgctta tgaaccaatc
    38941 tgggcaatcg gtactggtaa atcagctgac gcacaaatcg ctgacgaaat ttgtggagtt
    39001 gtacgtcaaa cagttgaaaa actttatgga aaagaagtat cagaagcagt tcgtatccaa
    39061 tacggcggtt ctgtaaaacc agaaaatatt gctgaataca tggcaaaaga aaatgttgac
    39121 ggtgctttag tcggtggcgc aagcttgcaa gcagattcat ttttgtcatt gttagacgct
    39181 gtaaaataat tgttgaaaat ttgcgaaaaa tgcaagaaat gtttgcacta atgagcaaaa
    39241 ttaactaaaa tcgtatatga gggaaatccc ttaggaaaaa acaaactcaa aggagagaca
    39301 aaacatgtca attattactg atgtttacgc tcgcgagatc ttagactcac gtggtaaccc
    39361 aacaatcgaa gtagaagtat acacagaaag cggagctttt ggccgcggaa tggttccttc
    39421 aggtgcttca actggtgaat acgaagcagt tgaattacgt gacggagaca aagctcgtta
    39481 tggcggtaaa ggtgtaacta aagctgtcga caacgtaaac aacatcattg ctgaagcaat
    39541 cattggctac gatgttcgtg accaaatggc tatcgacaaa gctatgatcg ctttagacgg
    39601 aactcctaac aaaggtaaat taggtgcgaa cgctatttta ggtgtttcta tcgctgttgc
    39661 acgtgcagct gctgactacc tagaagtacc tttataccac tacctaggcg gattcaacac
    39721 aaaagtattg ccaacaccaa tgatgaacat catcaacggc ggatcacatg ctgataactc
    39781 aatcgacttc caagaattca tgatcatgcc tgttggcgct ccaacattca aagaagctct
    39841 acgtatgggt gctgaagtat tccacgcatt agcttcaatc ttgaaagctc gcggattagc
    39901 aacttctgtt ggtgacgaag gtggatttgc tccaaacctt ggttcaaacg aagaaggttt
    39961 tgaagtaatc atcgaagcaa tcgaaaaagc tggctatgta cctggtaaag acgttgttct
    40021 tgctatggat gctgcttctt cagaattcta cgacaaagaa aaaggtgttt acgtactagc
    40081 tgactcaggc gaaggcgaaa aaacaactga cgaaatgatc aaattctacg aagaattagt
    40141 ttctaaatat ccaatcatct caatcgaaga tggattagac gaaaacgact gggacggatt
    40201 caagaaatta actgacgtat taggcgacaa agttcaatta gttggtgacg acttgttcgt
    40261 aacaaacact caaaaattat ctgaaggtat cgaaaaagga attgctaact caatccttat
    40321 caaagtgaac caaatcggta cattaacaga aactttcgaa gctatcgaaa tggctaaaga
    40381 agctggctac acagcagttg tatctcaccg ttctggtgaa acagaagatt caacaatctc
    40441 tgatatcgct gttgcaacaa atgctggcca aatcaaaact ggttctctat cacgtactga
    40501 ccgtattgct aaatacaacc aattactacg tatcgaagac caacttggtg aagttgcaga
    40561 atacaaaggt ttgaaatctt tctacaactt aaaaaacaaa taattctttt ctaatgaaga
    40621 tacatgatcg actattgaaa gatagtcgag tatgatcact tagttaagta atcgaactct
    40681 ctacttgctg aaatatcagc aggtagagag t
//
LOCUS       DAZKNM010000009        39048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000009 DAZKNM010000000
VERSION     DAZKNM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.009"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1354
                     /locus_tag="ACG8M9_000388"
     CDS             41..1354
                     /locus_tag="ACG8M9_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0377188.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            1682..3304
                     /locus_tag="ACG8M9_000389"
     CDS             1682..3304
                     /locus_tag="ACG8M9_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0377189.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            3498..4994
                     /locus_tag="ACG8M9_000390"
     CDS             3498..4994
                     /locus_tag="ACG8M9_000390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0377190.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            5044..6819
                     /locus_tag="ACG8M9_000391"
     CDS             5044..6819
                     /locus_tag="ACG8M9_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0377191.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            6877..8772
                     /locus_tag="ACG8M9_000392"
     CDS             6877..8772
                     /locus_tag="ACG8M9_000392"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0377192.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            8769..10226
                     /locus_tag="ACG8M9_000393"
     CDS             8769..10226
                     /locus_tag="ACG8M9_000393"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0377193.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            10810..11280
                     /locus_tag="ACG8M9_000394"
     CDS             10810..11280
                     /locus_tag="ACG8M9_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0377194.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKKIKFFKQISCMYN"
     gene            11346..12437
                     /locus_tag="ACG8M9_000395"
     CDS             11346..12437
                     /locus_tag="ACG8M9_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0377195.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            12467..13144
                     /locus_tag="ACG8M9_000396"
     CDS             12467..13144
                     /locus_tag="ACG8M9_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0377196.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            13476..14771
                     /locus_tag="ACG8M9_000397"
     CDS             13476..14771
                     /locus_tag="ACG8M9_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0377197.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            14761..16221
                     /locus_tag="ACG8M9_000398"
     CDS             14761..16221
                     /locus_tag="ACG8M9_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377198.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            16357..17853
                     /gene="glpK"
                     /locus_tag="ACG8M9_000399"
     CDS             16357..17853
                     /gene="glpK"
                     /locus_tag="ACG8M9_000399"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0377199.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            17867..19690
                     /gene="glpO"
                     /locus_tag="ACG8M9_000400"
     CDS             17867..19690
                     /gene="glpO"
                     /locus_tag="ACG8M9_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0377200.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            19691..20407
                     /locus_tag="ACG8M9_000401"
     CDS             19691..20407
                     /locus_tag="ACG8M9_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0377201.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            20713..22642
                     /locus_tag="ACG8M9_000402"
                     /pseudo
     CDS             20713..22642
                     /locus_tag="ACG8M9_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            22768..23322
                     /locus_tag="ACG8M9_000403"
     CDS             22768..23322
                     /locus_tag="ACG8M9_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0377202.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24052..24366)
                     /locus_tag="ACG8M9_000404"
     CDS             complement(24052..24366)
                     /locus_tag="ACG8M9_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0377203.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(24380..24640)
                     /locus_tag="ACG8M9_000405"
     CDS             complement(24380..24640)
                     /locus_tag="ACG8M9_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0377204.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            24810..25124
                     /locus_tag="ACG8M9_000406"
     CDS             24810..25124
                     /locus_tag="ACG8M9_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0377205.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            25126..26781
                     /locus_tag="ACG8M9_000407"
     CDS             25126..26781
                     /locus_tag="ACG8M9_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0377206.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            26864..28999
                     /locus_tag="ACG8M9_000408"
     CDS             26864..28999
                     /locus_tag="ACG8M9_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0377207.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            29174..29683
                     /locus_tag="ACG8M9_000409"
     CDS             29174..29683
                     /locus_tag="ACG8M9_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377208.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            29680..30984
                     /locus_tag="ACG8M9_000410"
     CDS             29680..30984
                     /locus_tag="ACG8M9_000410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0377209.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            31092..31949
                     /locus_tag="ACG8M9_000411"
     CDS             31092..31949
                     /locus_tag="ACG8M9_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0377210.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(32061..32381)
                     /locus_tag="ACG8M9_000412"
     CDS             complement(32061..32381)
                     /locus_tag="ACG8M9_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0377211.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            32535..32930
                     /gene="dhaM"
                     /locus_tag="ACG8M9_000413"
     CDS             32535..32930
                     /gene="dhaM"
                     /locus_tag="ACG8M9_000413"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0377212.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            32905..33915
                     /gene="dhaK"
                     /locus_tag="ACG8M9_000414"
     CDS             32905..33915
                     /gene="dhaK"
                     /locus_tag="ACG8M9_000414"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0377213.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            33943..34548
                     /gene="dhaL"
                     /locus_tag="ACG8M9_000415"
     CDS             33943..34548
                     /gene="dhaL"
                     /locus_tag="ACG8M9_000415"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0377214.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            34695..35360
                     /locus_tag="ACG8M9_000416"
     CDS             34695..35360
                     /locus_tag="ACG8M9_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0377215.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            35643..36302
                     /locus_tag="ACG8M9_000417"
     CDS             35643..36302
                     /locus_tag="ACG8M9_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0377216.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(36379..37989)
                     /locus_tag="ACG8M9_000418"
     CDS             complement(36379..37989)
                     /locus_tag="ACG8M9_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377217.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(38002..38883)
                     /locus_tag="ACG8M9_000419"
     CDS             complement(38002..38883)
                     /locus_tag="ACG8M9_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0377218.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
BASE COUNT        12962 a         6717 c         8343 g        11026 t
ORIGIN      
        1 cggactttat ttataatgtt cggatttagg gggatggaag atgaaggaaa aaatactttc
       61 ctacttcgaa atagaaaaat tgaacaccac ggtaaaacgt gaggtactgg caggatttac
      121 aacatttatc tcaatggctt atatcttatt tgtaaatcca actgtattag gtgcttctgg
      181 aatggatgaa ggagcagtgt ttactgcgac tgcattagcc agtgcgatcg gctgtgtttt
      241 aatgggggta ttagcccgtt atccaattgg aacggcacct gctcttggga tcaatgcttt
      301 ttttgcttat tctgtttgtt taggaatggg gattccatgg gaaacagcat tagccggcgt
      361 attcgttgct tctttgattt ttattttgat cacaatcttc aagctgcggg aaatgatcat
      421 tgatgcgatc ccagcagatt tgaaatatgc gatttctggc ggtatcggtc tatttatcgc
      481 tttccttggt ctgagcgaag gtgggatcat cgtttcgaat gaatctacat tagtaggatt
      541 aggatcactc aacgttggtt ctacttggtt gacgattttt ggattagtcg taactgctat
      601 cttgcttgtt cggcgtgtac caggtggcat cttcatcggg atggcagcta caacgatttt
      661 aggtctggtc acaggtttga ttccaatgcc ttctgaaatt gttgccgcag caccaagttt
      721 gaaaccaaca tttttagttg cattaaaaca tgtaggggac atcaattctc ttcaaatgtg
      781 ggtcgtagtg ctgacattct tgcttgtgac tttctttgat acagcaggaa ctttagttgg
      841 attagcaaac caagcaggtt tcatgaaaga caacaagatg cctcgtgtag ggaaagcttt
      901 agcttctgat tcaacagcta tgcttgcggg ttccttattt ggtacttctc ctgttggtgc
      961 atttgtagaa tcatcagctg gtatcgccgt tggaggacgt tcaggtatca cagctattac
     1021 tacagggatt ttcttcttgt taggcttgtt cttttctcca ttactatcag ttgttacttc
     1081 acaagtaact gcaccagcat tgattatggt aggagtattg atggcacaat ctttgcgaca
     1141 aatcgaatgg ggaaacttag agattgcgat cccttcattc ttgatcttga tcggcatgcc
     1201 tttgacttat agtatttcag atgggattgc attaggtttt atcttctatc caattaccat
     1261 gttagcagct aaaagaggaa aagaagtatc tccaatcatg tatggtctgt tctttgtatt
     1321 tgtgggattc atgtggatat tgaatgtgaa ataaagaggt tgtgaaatca gcgttctggc
     1381 ctgagtatta gagaaaaaaa cgaaaaaata gtttcttata ttttgccgtt ttttgagcta
     1441 ataccaaggg acagctgagt gaacaccgtt tataaagaag ttgtgaaatc agcattcttc
     1501 cctgcataaa tcaatccttt caaaaaacag ttgagggaat ttcctcagct gtttttttgt
     1561 tttattctgt taccgataac aaggtgcaat agctatttga aagcgttaca atttttagtg
     1621 agagagacta aaataaatga ttaagcaaaa aatagaaaca tccatttata ggaggaaaat
     1681 catggaacaa aaatggtggc accattcagt tgtttatcaa atctatccaa gaagttttca
     1741 agattcaaat caagatggta ttggagattt gcgggggatt atttccaggt tggattatct
     1801 ggcgtattta ggtattgatg caatttggct ttctccagta tacgagtctc cgaatgatga
     1861 caatgggtat gatatcagta attatcaagc aatcatggca gaattcggta cgatggaaga
     1921 aatggaagaa ctgattgaag aagcaaagaa aagaaatatc cgtatcatca tggatctggt
     1981 ggtgaatcat acttctgatg aacatccgtg gttcattgag gcccgtaaaa ataaagaaaa
     2041 tccttatcgg gactattata tttggcgtga tccggtcaat ggaaaagagc ctaatagatt
     2101 gcgctcaata ttcagtggtt ctgcttggca gttggatgaa aaaacggggc aatattttct
     2161 gcacttgtat agcaagcgtc aacctgattt gaattggaaa aataaacagg ttcgccaaga
     2221 gatttatcaa atgatgaatt tctggatcga taaaggtatt gggggctttc gtatggacgt
     2281 gatcgatatg attggcaaag aaccggataa agaaatcaca agtaatggac ctaaactgca
     2341 tgagtatctt caggaaatga atcaagcaac ttttggtgac aaaaatctga tgactgttgg
     2401 cgaaacatgg ggagcaacta ccgaaatcgc taaactttat tccaatccta aaagaaatga
     2461 attatccatg gtcttccaat tcgaacacat aggattagat cagcaagaag gcaaagacaa
     2521 atgggatttg aaacccttga gtatctctga actgaaaaaa gtattatcca aatggcaaac
     2581 atctttaggt gatgaaggat ggaatagttt attttggaat aatcacgatc ttccgcgaat
     2641 tgtttctcgt tggggaaatg acgatgtgta tcgaaaagaa agtgccaaga tgtttgctat
     2701 cttgctccat atgatgaaag gcacccctta tatctatcaa ggagaagagt taggcatgac
     2761 gaattgccca atcgatgata tcgcggaagc aaaagatatt gagacaatca atatgtataa
     2821 cgaaaggatt tcttctggat ttacaaaaga ggaaatcttg gaatcaatca aggcgaaagg
     2881 aagagacaat gctaggacgc ctatgcaatg gaacgcccgt gaacatgctg ggtttactac
     2941 tggaattcca tggcttcgag tcaatccaaa ttataaagaa atcaatgcag aagaagcgtt
     3001 agcagatcaa gattctgttt tttatatgta caagaaattg atcgatttgc gtagaaaaca
     3061 tgacatcatc gtctggggag actatgaatt agtggaaaag acgcctgagc atgtctttgc
     3121 ctattctcgc atatatgaag accagcaatg gctcgttatt tgtaatatgt ccagtgaaaa
     3181 gcagacgttc actattccac gtgaagcttt aagggttatt gtgtcaaatt atcctctgaa
     3241 acagttacca gacggcaaag ttgatttacg accttatgaa gcatttgtgt tggaagtaaa
     3301 ataaatcgtt tgcttagatg gttaacgagt gttttctttc ggggatcgtc ttatgtattt
     3361 gttatatata aatattaaaa atgaagagta gcattcaatt tttttcttct cttctgccga
     3421 aatagttaga atatcttgtt ttgatgaatc gagctatact tcaagtaaga aattgttgat
     3481 agcgaggaga gatagttatg gatcaggaaa tatttccaga aaatttttta tggggcggag
     3541 cagtagctgc taatcaatgt gaaggtgcat ggttggaaga cggcaaatta cctaatgtga
     3601 cagatacgct tatcgggatc atgaaccagc atcctagtat tcagtggaat gaagaaaaaa
     3661 agatttggga gattgcttta gacgaatcac tccattatct ttctcatgaa gcgattgatt
     3721 tttatcatcg atttgaagag gatattcgtt tactgaagga gttaggttta aaagcttttc
     3781 gaacaagcat ctcatgggca cgaatatttc caagaggaga cgaacagaag ccaaatgagg
     3841 ctggtctcgt attttatgac agactaatca atacattaaa tcgatatgat atcgagccag
     3901 taattactct tagtcattat gaaaccccct tagcattggt aggagagtat ggggggtggc
     3961 aaaatcgaaa attaatcgac ttttttgaat tttatgcgca gaccgtcttt gaacgctacc
     4021 aagggaaagt gaaatattgg atgaccttta atgagatcaa taatgctttt cgtatgcctt
     4081 atgcagctgc tggattggtt acattccctg ctagagataa acttgaaccc attgctacac
     4141 taacaaaaaa agtgatttat caagcctgtc atcatatgtt tctagcgaac gcccgtgcta
     4201 ctcaattatt aaagaaaatc gatccaaatg ctaagatggg tattatgtgt agcttttctg
     4261 cattagcgac ttatgcatat gactgtgatc cagagaatgt atttggttca ctgcaattta
     4321 aacgcaattc atggtttttc tccgatgtca tgtgccgagg gcattatcca gcttatattt
     4381 acaggacatg gaaggaagaa gactgtgcac cagtaatgtt agatggagac gaacagtgct
     4441 tgcaagctaa cacggcagat tatattgcat ttagttatta tcgtagcgct gtctataaaa
     4501 aagaagcaga aatgcgcgta gatactggcg gagcaaatgg tttcgataat ccctttttga
     4561 aggaaaaatc tcccgaacct tggagttggc caattgatcc tctagggttt cgttatgtca
     4621 tgaatgaatt gacagaccgt tatgaattac cattattcat agttgaaaat ggcattggac
     4681 tagatgaagc accagatgaa ttgaatcgaa tccacgatcc aaaacgttgt gagtatctaa
     4741 aaatacatct tcaggaaatt gctgaggcaa taaaagatgg ttgccaaatc attggttact
     4801 tatggtgggg accaatcgat atcgtatcgg caggaacagg ggaaatgagg aagcgatatg
     4861 gctttattta tgtggataga caaaatgatg ggagcggaag ctttaggaga ttgaaaaaag
     4921 atagttttga atattataaa caaattatcc agtctaatgg taaaaacctt gaatatagat
     4981 ctttcttcaa atgattttta aacctctgca agtgactatt gtcatttgag aggtttttcc
     5041 tgtatgttta taacaaagag gaggtgtaag caaatggact taaataaacg acaactccaa
     5101 attcttgtag tattaagaaa taagaatcat tggataacaa gcgaagaatt agctgaagct
     5161 ttaggtacca acaagaaaac gatccaaact gaaatcaaaa atatgcttgc tatctacgag
     5221 aagaaaatca ttatccagtc aaataaacgt tcaggttatt ttttagaatc aattgaatct
     5281 gaaactaaac agcaaatcat acaagaggtg acgaaacata aaatctattc cagcatggat
     5341 ttccgagctt caactattgt gacgtactta ttgtttcaaa aagggtatgt cagtatgcaa
     5401 aagatagcag acattttctt tctttcaaaa acagccgtat cattacaaat aaaaacgatt
     5461 ttgcgttgga tcgagcgcaa tcctaaatta gaactggaag tttcgtcgac aaaaggacta
     5521 aaaatcattg ctgaagaaaa ttcaaaaaga attttcttat ctcttgttgg aacagaaacg
     5581 gtgatccaac acgcaaggtt tcctcaacaa atcagtacaa ttttttccaa tatcatgccg
     5641 actgtccaaa agacattaaa agaggttctt atatcatata attatattgt ttcaggagag
     5701 gacttcatac gattttctcg gtatctcgtt ttgaccattc ttcgaagaga agaactttta
     5761 gaaaaaacaa acaggtatac attatttatt ccgatggtag agaccctcac aaaaaagtta
     5821 tcatcagaac taccctattc tttttccaat caagaaaaac aagcaattga aaatcgacta
     5881 ttagagttaa attatttgat gattaatgat gaaaaaaaca aagaaataga gaagaatcta
     5941 agagcatttg agcaaacgat tattcgtttt ttagatcttc ctgtttcgtt attgttccat
     6001 gaaacagaat ttttgctaac ccatatcaaa caaatgaaaa cgaggatgga tgccggacat
     6061 aataccatga atcattttgt acaaaagatc atctcccgtt atcctttaga aatttattta
     6121 attcggcgtt tcttcccaat acattttcaa ttacgtccca atttagctga aagcgcttat
     6181 tttgttttgt atcttgccga agcgttgaat ccttttcgaa aacaagggga gatactgatt
     6241 gttagtaacc agccaattag tattttgaat acactaaaaa aacagattca acaaagtatg
     6301 aacatgtgga tcaaagaatg ccggatagaa ccagtgtatc ttttcaaaaa tcaatcaaac
     6361 aaaataacgg aatatgatgc aattttgact actgagcaag agcttatttt tgagtgggat
     6421 gatttgattt atctacctag tgttctagat gagtttgaat cttgggagat ttatcagatt
     6481 ttgcgtgaaa agctgaccat agttgaagaa aaaaagaaaa caaaactgct tgatcattac
     6541 tacaaagaag aaaatcaaat aaaaatccat aaaaagatag attccattca gcaactaatc
     6601 tctcctagtt ctgacctagt tatgcttccg ttaaatgccg agactttgtt gtgctgtctt
     6661 atcaaaagcc aagtagaaac aaaaatcaca gagtatcagc ttgagatacc acttatacat
     6721 cagcaaagga agatcaagcg tgtttttttt gtctcctttg actataagag aaacgatata
     6781 cttgttttct ttcaagcagt gagtgatttg ctcggctaag taaaccatac acactattgt
     6841 acagtttcgt tcgctattag aaggggtact aattgaatgg agaaaccgtt ttctaagaac
     6901 tatacttatc ttgtaagaag gattgctacg gcattaatta acaaggggga agtaaaaatg
     6961 gactattcac aattagcgaa agatattgtc cgatttgtcg gaggcgaaga aaatgtcagc
     7021 aatgtttacc attgtgcaac aaggttacgc tttaccctaa aagataataa gaaggcagat
     7081 aaggagaagg tagagcaact agaaggtgtg atcactgtag tcgaagctgg aggaatgttt
     7141 caagtcgtag taggaaatgc agtaaatgaa gtgtatgacg tattatccaa acaaatgaaa
     7201 ctagaagatg atgcatctag tggaaagaga ggaactgaga aaaaaggcat tttaaactct
     7261 tttatagaca tgatcgccgc tgtttttgcc ccgactttag gcgtcttggc aggaagtggt
     7321 ttgatcaaag gagtactcgc cttatgtacg agtttgaatt tattaactac tgaatcagga
     7381 acttatatca ttttgaatgc agcagctgat gcatttttct atttcttgcc tatatttttg
     7441 gcatatactg ctgcaaaaaa gttcaataca gatcgtttca tcgctatggt tattgcagca
     7501 gctctcgttt atcctacgat tgtcagtgca tatagtgata gcataacctt gcgatttcta
     7561 gggatgcccg tgattctagc aaggtataca agtacggtta ttccagcaat tttagctgta
     7621 tgggttcttt cttatattga gcctaagatt cgcaaaagct tgcacgaaag tattcgaaat
     7681 ttattgacac cattcatttg tatcatcgtg atggtcccaa ttattttatt agttgtaggt
     7741 ccaattgcag attatgcaag tcagctgatt gcagctggat atttagctgt ttataacttt
     7801 agccctgttt tatctggtgc tgttatcggc ggattttggc aagtgttagt gatttttggg
     7861 ttacattggg gacttgtacc agtaatgacc aacaatcttt ctttctacgg tcgtgatacg
     7921 ctaggtccag catgtatgac tgccgtagct gcgcaagcag gagcagtact tggggtattt
     7981 ttgaaaacga aaaataagaa agtaaaaagt ttatctttat ctgcatttat cacagcatta
     8041 tttggaatca ctgaacctgc tgtttatggt gtgactctta aatataaacg accattttac
     8101 attgcttgta tatgtggagc tatatttggt ggagtagctg gtgccgcagg agccggtgct
     8161 ttggcagttg ctacaagaag tattttgtct ttcccgattt atattggtga aggatttgtt
     8221 tggctagttg ctagttattt cttagcgatg atcagttcct gtatgctaac ttttcttttc
     8281 ggctacaagg atgaaatcga agaagaatcc tcaaaggata tagtgttaag tactcctgca
     8341 gcaggtgaaa taattgattt atctgaagta aacgatccaa catttgctag tggatcttta
     8401 ggagaagggt ttgcgatcat tccaacggac ggaaaaatct acagttctgt aaacggggaa
     8461 gtttcaacag tttttccaac gaaacatgca attggggtag tcagtgaaga aggagcagag
     8521 atactgatcc atatcggtat tgatacagtg aatttaaatg gcaaatactt tcaaagtgca
     8581 gtatctgatg gaaaaaaagt acgtaaaggc gatttattga tggaagtaga tttgcaagaa
     8641 ttaatcaaag agggatatga tccgaccaca atggttattg ttacaaatag tagtagattc
     8701 aaaaatatca ttactagtca taagaaaaat aatcaaacaa gaaaactagc aagtttaggg
     8761 ggatcgatat gaaaaaattt ccagaaggat ttttatgggg cggagcgaca gcggccaatc
     8821 aatgtgaagg tgcatgggat gtagatgaaa aaggattatc tgtgtctgat gtgtatacat
     8881 tcgacagcga tttacctaaa gaaaaatgga cagatcaatg gcatatgatg acgcatcagc
     8941 aagtaaaagc ggcgcaggat cccaacagca agtattatta tcctaaaaga cacggaaatg
     9001 atttctatca tcactttaaa gaagacatcc gtttatttgc tgaaatgggt tttaaatgct
     9061 atcggatgtc tatcgcatgg acaagaattt ttcctcatgg tgatgaagaa acacccaatg
     9121 aagcgggatt atttttttat gatcaagtgt ttgacgaatg tctaaaatat gggattgagc
     9181 cagtcgtttc tctttcccat tatgaaatgc cgttatattt ggttacagaa tatggtggat
     9241 ggcctaatcg aaagcttatt cagttttacg ttcgtttcgc tgaaacagta ttcaaacgct
     9301 ataaaaataa agtaaaatat tggatgactt tcaatgagat caactgtgta aaacatcacc
     9361 cttttgttag cgtaggagta attgaagaaa accatcccca tttggaacaa gcgaaatacc
     9421 aaggtgcaca ccatcagttt atagcaagtg ctttagctac aaaagcttgt catgaaatta
     9481 tcaaaggttc acaagtcggt tgtatgatca gctatcagct tcttgttccg tattcttgtg
     9541 atccggatga tattcaaaaa acaattgagc agcaaagaac ttctttgttc tttagtgatg
     9601 ttcaagcaag aggatattat ccagcgtata cgcaacgaat gtttgaagaa aaaggtgtca
     9661 acttaaaaat agaagtaggt gatgaagaaa tcttagcagc ctaccctgtt gattttgttt
     9721 cttttagcta ctacatgtct agtgcagtca gtgctcatcc agaaaattgg gaaggggtcc
     9781 aaggaaactt aataactgga ggaattaaaa atccgtatct tccatcaagt gaatggggct
     9841 ggcagattga tcctaagggt ttgagaattg ctttgaatca attatatgat aggtatcaaa
     9901 aaccactgtt catagctgaa aacggactcg gagctataga caaagtagaa gaagatggaa
     9961 ccgttcaaga cgattaccgc attgactatt tgaagcagca tattgagcag atgaaggaag
    10021 ctatcaaaga tggagtagat ttatttggtt atacgtcgtg gggctgcatt gatatcgtca
    10081 gcgcttctac ttcacaaatg tcaaaacgtt atggatatat ctatgttgat caagatgacg
    10141 aagggaatgg cacaaagaaa cggttaaaga aaaaatcatt tgattggtac agacaagtca
    10201 tccaatcaaa tggcgaaatc ctataataaa aggaaataag ggactgtgac aaaagtcttg
    10261 tcacagcccc ttctttacga ataaacagta ttctcctgcc aactcctcga tcacaagtca
    10321 ccatagtcat aaaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagttca
    10381 acggcaggct cacgacctct caataaacgg tgtacaatca gctgaccatc ggcattagct
    10441 caaaaaacga caaaatatga ggaactattt ttgtttggct gttcttatta cttgatgcag
    10501 gacggctttt tcacaacctc tgatataaac ggtgttcaaa agccagtttt tcggtattaa
    10561 gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttcttc atactcaaaa
    10621 ctaaacgact ttttcacaac ctctgattca cggtgttcca gaaggagttt tgtcgaaaac
    10681 aagccgaaat tctttgaaga tggctacttc tttttttcaa atctagacag aggaggcgga
    10741 cattattccg tgttttttga caaaaaataa aaatcatact atgatatgta cgccaatgga
    10801 ggggatgaaa tggaaaagcc aattgaaaaa gcgttagatg aatgtctttt ctttagcgtg
    10861 aaaaagctag atcgcatgtt gaataaatta gctgatgaag cgtttagaag gacaggtctg
    10921 gcaccaactt atggttttat tttattgata ttaaaagaaa aagatggaat accacaaaaa
    10981 gatattgcac aaatgctcta ttccgcccca tcaacgattg ctcgttttgt tgaaaaatta
    11041 gaatataaag gatacgtcaa aacagttagc gaaggtcgct tgtcattagt ttatcttaca
    11101 gaacaaggac gccaatttgc taaggaagtc gatggtagct gggaagaact tcatcagtca
    11161 tacaaagcag ttttgggaaa tgaagcaagt aaagaattag cccagacaat gaatgatgca
    11221 acagataagt tacaaaaaaa aataaaattt tttaagcaaa ttagttgcat gtacaactaa
    11281 tcgagtggtt taatgataga tgattctcga taggttaatc agagcgaatt atgggaggaa
    11341 tgacaatgtc acaatttgat caatcaatta ctttaccttc aggagttcaa ttgaagaacc
    11401 gattgatgct gtcgcctatg acgacacaac tcagcttttt caatggagtg atcaccgaag
    11461 atgagatcac ttattataaa caacgttcta aaggtcttgg tgctgttatt actggtgctg
    11521 caaatgtcca agatatcgga aaaggctggc caggtgaact cagcatcgct catgatgaaa
    11581 tgattcctcg attaagtgag ctagcgaaag caatccaagg agaaggcgca aaagcgatta
    11641 ttcagatttt ccacggtgga cgaatgacaa gtcgagcaac tttatctgga gaacagcctg
    11701 tatctgcaag tgcagttgca gcaaaacgtc ctgatgcgga aacaccacga gcaatgagtg
    11761 aagaagaaat cgttgaaaca atccaagcat ttggcaaagc cacacgtaga gcgattgaat
    11821 caggatttga tggaatcgag ttgcatggag caaatacgta tttgatccaa caatttttct
    11881 ctccgcattc gaatagacga gaggatcaat ggggcggtag cttggaaaat cgttatcgtt
    11941 ttattgaaga attattaaca acggttttct cagcggttga acaatatgct aaaaaaccat
    12001 tcatcgtagg gtatcgtttc tcaccagaag aatatgaaac accaggtatc cgttttgaag
    12061 acacgatctg gcttcttgaa cgattacgtg agtcaaaact agattatctg catgtttcat
    12121 taaatatgta tgatcgaata gctcgttctg ataaatacaa cgataaatcg attcttgaag
    12181 tggtccataa cacgattcaa ggaaagatcc ctttagttgg tgtcggtggg atccgcaatc
    12241 gcgaagatgt agagcaggta ttgaaatatg cagaacttgc agcaattgga cagcaaatga
    12301 tcgtcgatcc tgactgggca gagaaattat tggaaaaccg tgaggatgaa tttgtaacga
    12361 aaccttttga aaaagcatat aaagagcttt atttgccaag cccattatat aatttcttga
    12421 aagcacgtta tcaataacgc aatttttata gaaagaggat gggaaaatga aagtattgat
    12481 cgtattaaca aatgtagaaa agtacgaaaa gatcgagcgt cctacaggtt tgtggttaag
    12541 tgaactgact catttttatg atgtattagt aaaaaatggg atcgatgcag attttgtcag
    12601 tcctaaaggc ggatatgtac ctttagaacc gcaaagtgtt ttgaatatgg atgaaatcga
    12661 ttgggcctat tatgaagatg aagatttcag aaatcgggcc ttgggaaata ccttacgtcc
    12721 tgatgatgtg gatgcaaatg attatgcagc ggtttattat gctggtggcc acggaacaat
    12781 gtgggatttt ccagaagcaa tagatcttgg gaaagtcgct tcacaaatct atcgaaatga
    12841 agggctggtc tcagctgttt gtcacggtgt tgtaggattg ttgccagtta cagatgagtc
    12901 tggaaaatct attttagctg gacgtacagt gactgggttc agtaatgaag aagaggaagc
    12961 aaatggtaca acgaatgaag taccattctt agcagaagat gctttgaaag aaaaaggagc
    13021 aaactatcag tctggagcag ctttttcaga agtggttcaa attgatgggc ggcttcttac
    13081 agggcaaaat cctcaatctg ctcatcaatt aggcgaagca gttgttgaag cattaaagaa
    13141 ttaaaaataa atgatgtgtc tattctaact attacattac acacgctaaa actcgtcttt
    13201 tcgttctttt ctgtttccta tttggacaga ggtacgtaaa aggcgagttt ttttctttta
    13261 taagagtaca ttttttgaaa catctcttca gaaatgatac tcttcaatta ggtccactag
    13321 tacattattg agaagcagac ctagaacacg gtatgatgga gagggttgat tctgctgcat
    13381 cgctatgtat cataaggttg aaagcgagtt atgaatactg tgttttctga ttgagaagtt
    13441 agcagtagaa caccttataa gaaagaggaa tgcagatgaa cattgtaatt gtcggcgggt
    13501 cgtttgcagg gattcatgcg gctatttatc ttcgccaatt gatgccaaca agtgaaatat
    13561 atttgattga aaagcaatcg aagttaggat ggattcccag cgggttcaat ttgatattaa
    13621 aaggaaaggt taccagtgaa aaacaacttt cttggatcac gaaagaagaa ctgactgacc
    13681 gctatcgaat ccatgtttac acagaaaaga cagtaatagg attgaaagaa aaaaaagtcg
    13741 ttttagcagg aggaaaaaaa ttggattttg atcgtttgat tctagcgact ggttcaaatc
    13801 aaaagtttcg aaatatttct gcagaatcaa cgcttattca cccagttaaa aagatacaaa
    13861 acgtaacggc tcttcagcaa aagatcatga atgcaaagaa aattgcaatt attggtgcag
    13921 gacaagcagg aatcgaaata gctgaagggt tggcatcaag aaaaaaacaa atccatcttt
    13981 acgaaagccg aaagtcaatt ctttttcgtt atctagatcc tgaaatgaca gaaccgttag
    14041 taaaggaaat gaaaaatcaa gggctttcac tttttttaga agaacaagtt gtgtctttga
    14101 ccgaaaatga aaccgctatt gtagagacgg aaaaaagaaa ggagaactat gatcttgttt
    14161 tgctggctaa tcattctcgt cccgacaatc agatgtggga agaacaatta acattgaatg
    14221 atgatggaac gatctgggta gatgactact tgcaaacgtc tcaaaaagac gtttatgcga
    14281 ttggcgatgc gatccaagta acttttcgtc caacaaatga aaaaatgtat gtttctctag
    14341 taaataacgc tgtccgtaca gcacgaaatg tcagcaagac aattagtggc tcacagacaa
    14401 aggatcaggg aacctatcgt ccagtcggca atcagtggtt tggctatttc ttaggaagcg
    14461 taggtttgac agaagaagag agcatttttt atccccaaaa aattgaaaca ggatacgtag
    14521 actacaagct tacagcaata aatcaaagca aagttaaact caaatttcta tttgattctg
    14581 aaaaaaagat tcttggagca cagctccaaa gcaaaaaaga gatgttccac cttcttgacc
    14641 aactgacaat tgcagtagaa gaaggatgga cgtttgaaca gttagaagaa catgagctat
    14701 ttttccagcc agaataccgt gtgccagata tggtgtggtc aaaggtggcg aatactgcct
    14761 atgaagattg aagcattatt agaaagaaaa gagcagttac agatcctgat tttgcgaaac
    14821 cttgttctgc aagggggaac agcctctatc aatggtttga gagagcatgt ccaactgtca
    14881 aaagcctcgt ttgatcaata tttggaagat attgaattga ttggccgaat gatggaaaaa
    14941 aaagtagaag tacagcgaaa tgagtatcag gcgctgcttg tgttggacga agatgtaagt
    15001 ttggaaaaaa ttctattgtt tttattgcag gaaagcttga agtttaagat gctcgtctat
    15061 ttattagaac atcagcaagt atccatcgtt cgattagcga cagcgtttaa tatcagtgag
    15121 tcttctgttt ttcgaaagat caaagaattg aaccagctct tagaggaatt tggattacaa
    15181 atcaaaaacg gtcaattata cggagaagag ctacagatac gatattttta ttatgaacta
    15241 ttccaatata tcccggaaga tcagcgaccg ctttttttgc aaaatacacc agaaaaaaga
    15301 ccgttcattc taggattgga ccgtgtatta gaaacaacgt ttacggcatc agcagaagca
    15361 cagattgctt gctggctcgg gattacgaaa aaaagactat tgaatgaaaa aagtacgtac
    15421 gcaactttga aagaaaagaa attactctat cagtcggacc ggctttatca agcaatcgat
    15481 ccaatcatcg tgatgcatct tagtcgaaca gcagcagaac tgaatgtata cgaaacaatg
    15541 atgttttaca gcttttttgt ttctttttcg atagtagacg aagaaatatt ttatcagtat
    15601 gatttgacac ggagcaaaaa gttaccaacc gctgttttag atacttacat tcgggaaacg
    15661 atgttatggc attatcgtcc tcgtcgtttg aaaatcaaag aagaaaaggc agtaggctac
    15721 caaatttccc agatcaacaa tgaatggttt ttctttgttg gaaagatcga agtctatgag
    15781 cgtgatcggc ttttggaaca gcagcagaaa atgctcgggc attccttaac acagttactg
    15841 gcgaaattac aggaaacagc ggtgcagcaa ttgccgagga aaagagctga agatagtgag
    15901 cttagttacc tgatgatcca gtatgccaat gttcttttga tgatcgattt ttatattgca
    15961 aaaccagtag tgattggaat cgatttagaa agtctgccga tttatcgtat tgcttttcaa
    16021 caatatctga tcagagaact gaggggaatc ggcggtattg agattggaag ttatgaagaa
    16081 ggtaaagaat atgatttggt gatcacattt tgccagagaa acaaaaaaca gtgtgaatat
    16141 tatctgtcag aatttgcgtc accttacgac attattcgat tgaaaagaag aatagagacg
    16201 ctgaaaaaag aaaagaattg attaaaaaat agcttcataa taaaaaactg tcaaaaaaag
    16261 aaaaaaagaa taaaattttg acagtttctt tttttaaaac agtagtatga taagagtagt
    16321 taaagaaagc gtttgcatat aatggaggga atcacaatga cagaatcaac gtatatcatg
    16381 gcgattgacc aaggtacaac tagctcacga gcaattatct ttgacaagaa aggaagacac
    16441 atcggcagtt ctcaaaaaga gttcacgcaa tatttcccgc aagaaagttg ggtggaacat
    16501 gatgcaaatg aaatctggaa ttctgtacag tcagttattg ccggagcgtt catcgaatca
    16561 gggatcaaac caaatcagat tgctgggatt ggcataacga accagcgtga aacaacagta
    16621 atatgggaaa aagatacagg acgtccaatc tatcatgcag ttgtttggca atccagacag
    16681 tcagctggaa ttgcagataa cttaaaaaat gaaggctatc aagaattttt ccacgaaaaa
    16741 acagggttag tgatcgatgc ctatttctca gcaacgaaga tacgctggat tcttgatcat
    16801 gtagaaggcg cgcaagaacg agctgaaaaa ggcgaattga tgtttggaac gatcgattct
    16861 tggttagtat ggaaactgac cgatggacaa gcacatgtta ctgattactc gaatgccagt
    16921 cggacgatgt tgttcaatat ccatcagcta gattgggatc aggagatttt agatttacta
    16981 aatattcctc gagtgatgtt accaaaagtc acttctaact cagaagttta tggttatacg
    17041 caaggttatc atttttatgg aagcgaagca ccaatttcag ggatggcagg agaccagcaa
    17101 gccgcattat tcggacaaat ggcattcgaa ccaggcatgg tcaagaatac ttatggtaca
    17161 ggttcattta ttgtcatgaa taccggtgaa aaaccgcaat tatccaaaaa taatttattg
    17221 acgacaattg gttacggcat aaatggaaag gtttactatg ctttagaagg aagtattttt
    17281 gtggctggtt cttcgattca atggctgaga gatggacttc aaatgttgca aaaagctagc
    17341 gattcagaag acgcagcgaa acgctctaca agcgaagatg aggtttatgt tgttcctgca
    17401 ttcgttgggc taggtgctcc ttattgggat caggcagcac gaggggctat gtttggcttg
    17461 acacgtggta cgacaaaaga agatatcatt aaagctactt tacagtcgat tgcttatcaa
    17521 gtgagagata tcatcgatac gatgcaagat gacactggaa tcaagattcc tgtattgaaa
    17581 gtagatggcg gtgcagcaaa caatgaatat ctgatgcaat tccaaacaga tatcttgaat
    17641 gtaccgatcc aacgagcaga aaacttggaa acaacggcgc taggggcagc atttttagca
    17701 ggattagctg tcggttattg gaaagataca gacgagatcc gcgaatttta tgaagctggc
    17761 aagctatttg aagttcaaat ggaggaagag cgccgagaaa aactttacaa cggttggaaa
    17821 aaagcagtaa aagctaccca agcatttgaa taaggaggaa atccatatgt tttcaaacaa
    17881 gacaagacaa gatagcattc aaaaaatgca gcaagaagaa ttggatctgt tgatcatcgg
    17941 tggcggaatc actggtgccg gtgtggcagt ccagtcagca gcatcaggaa tcaaaacagg
    18001 attgatcgaa atgcaggatt ttgcagaagg gacttcctct cgctcgacca aacttgttca
    18061 tggcggtatt cgttatctga agacatttga tgtggaagta gtagctgaca cagttggtga
    18121 acgtgcagtc gtacaaagta ttgccccaca catcccaaaa ccagatccaa tgcttttgcc
    18181 aatctatgag ggcgaaggag caacaacctt caatatgttc tcggtcaaag tagcaatgga
    18241 cctttatgac aaactggcaa atgtgacagg aaccaaatat gagaattata cgctgacacc
    18301 agaagaagta ttggaaagag aaccgttttt gaaaaaagaa gggctaaaag gcgcgggtgt
    18361 ctatctagat ttccgcaaca atgatgcccg tttagtgatt gataatatca aaaaggctgc
    18421 cgaagatggg gcttatctag taagtaaaat gaaagcggtt ggctttttat atgatggcga
    18481 tcaaatcgtt ggcgtcaaag ctcgtgacct gctgacagat gaagtgatcg aaatcaaagc
    18541 aaaattagtg atcaatacga gtggtccttg ggtagataaa gtacgaaact tgaattttac
    18601 gcgtccagtc tctcctaaaa tgcgtccaac taaagggatc catttagttg tagatgcgaa
    18661 aaaactgcct gtaccgcaac ccacatactt cgatacagga aagcaagatg ggcggatggt
    18721 ttttgctatt ccaagagaaa acaagactta ctttggtacg acagatacgg attaccaagg
    18781 agattttacg gatccgaaag tcacacaaga agatgtagat tatctattgg atgtgatcaa
    18841 tcatcgctat ccagaagcaa atatcacatt gaatgatatc gaagcaagct gggcaggact
    18901 tcgtccactg ttgattggta attctggttc tgattacaat ggtggagata atggatcgat
    18961 ttcagacaag agcttcaata aagttgtcga tacagtaagt gaatataagg aaaacaaagt
    19021 ttctcgtgct gaagtagaag acgtgttgaa ccatttggaa aacagccgtg atgaaaaagc
    19081 accttctacg atttccagag gtagttcttt agaaagagaa ccagatggtt tgctgacttt
    19141 atctggtggg aaaatcaccg attaccgtaa gatggcagaa ggagctttgc gattgatacg
    19201 ccaattatta aaagaagaat acggaataga gacgaaagaa atcgactcta aaaaatatca
    19261 gatttcaggt ggaaacttcg atccaactaa attagaagaa acagtgacag aattagcaaa
    19321 agaaggagta gcagccggtt tagaggaaga agatgctact tatatcgctg atttttacgg
    19381 gactaatgcc cgacgtatct ttgaattagc aaaagaaatg gcaccttatc ctggtttgag
    19441 tctcgctgag tcagctcggt tacgttatgg tttagaagaa gaaatggttt tggccccagg
    19501 cgattatctc attcgtcgta caaatcatct gttgtttgaa cgagatcagc ttgatgagat
    19561 caagcaacct gtgatcgatg caattgctgg atattttgaa tggacagaag aggagaaggc
    19621 gcaacagact aaacgtttag aagcattgat tgcagaatca gatctgcggg aactaaaggg
    19681 ggagaaataa atgaacagtg atatgactca aatcatgggg gagtttattg ggacactgat
    19741 cctagttttg cttggagatg gtgtgtgtgc ggctgtgaat ttaaacaaaa gtaaagcaca
    19801 ggcttctggc tggattgtca tcgcatttgg ttggggactc gctgtaacaa tggctgtcta
    19861 tatttctgga ttcatgggcc cagctcattt gaatcctgca gtctctctag ctatggcgat
    19921 gacaggggca attagttgga atcttgtcgt tcctttcatc attgcgcaag ttttaggagc
    19981 ttttgcaggg gccattcttg tttggctttc ttatttgcca cattggaatg caacgaaaga
    20041 tgaaagcgcg attttaggaa cgtttgcaac aggtcctgct atacggaatt atccagcaaa
    20101 tgtgatcaca gaattaattg gtacatttgt attagttctt gggttgctag cttttggaca
    20161 aaacgaattt gcacctggaa cgaacgtatt cgctgtaggt ggattgatct tagccatcgg
    20221 tctttcatta ggagggccaa caggatatgc aatcaaccct gctcgggact ttggtcctcg
    20281 attagcacat gcggtattgc caatagctaa taaaggaact tcagattggg catattcatg
    20341 ggtaccaatc gcaggtccaa tgatcggagc aattatcgcg gtaggtgtct attcattaat
    20401 ggtgtaaatc tataaaacat actaaagttt gacaaccaaa atgtgtcttg ctatagttaa
    20461 gtggaaaata aataagacct agaagaatgg ggtatgtttt gtgagtgtag ctggtatcta
    20521 atcagtaagt cgaatagcca ataaacaaag tttatttggt gtgcttatga aacggtacct
    20581 gtcaggaaaa caatatcttt gtattctaat aaggatagga catgactttt ttaagctgtg
    20641 ttctatcctt ttatttatct ctaggccctt atttattttg ggacaggtat ctcttcaaga
    20701 aaaggagaga atatggacaa cgaaatgatt agtcaattac aatttgatcg gatcaaaaag
    20761 gaaatccaag caagagcaat tggaaactat agcaaaaaac gaatcagcga tatgacggtc
    20821 tcgacaaacc tacaaactgt cttggatcgt caagaagaaa caagagaagc acgcttgatc
    20881 ttagaaagca gtcagcatgt tccatttatg gggttgccga ggatcgatgc actgacagaa
    20941 caagtcaaaa aagggttgat tctgcagcca accgatttga tcgaatatgc ggattttctt
    21001 agaagcagtc ggatgatcac aaagtttttt gataagaatc aataccagac acccttgttg
    21061 tttgcttata gcaaacatct tcctgatttg ataaatgtag aagagttgat tgaccagaaa
    21121 ataaaaaata ataaagtcag tgacgacgcg tcacgtaatt tgcgtaaagt acgcaaacaa
    21181 ttacaactaa tcgaaaaaga aatccagtca aaattactga aattcttgcg tcatccgaaa
    21241 aacaaagaaa tgatccaaga agcaatgatt gttcaaaaag gcgaatacta tacgattcct
    21301 atcaaagcca gctataaaaa taaaatagat ggaacgatta tcgatgaatc aaataaaaga
    21361 acgacggttt ttattgaacc aactgtgatt tctaaattga atgaacacta tcagttgtta
    21421 aaagctgaag aaatcagtga agaatatcaa gtcctggcgg cattgactgg tgctatcgca
    21481 gaaaacgaag aagtgatcga tttattgatc gaaacgatca cggtgttaga tatcatcttt
    21541 gctcgggcaa aatttagccg agaaatcaat ggaagcacac ctagaatcaa taaatcggaa
    21601 catatcatta tcaagcaagg cagacatcca tttttacctg agcatgcggt accattggat
    21661 gttgaaatcg gtaaagacta tcgaggcttg attatcaccg gagcaaatgc cggaggcaaa
    21721 acagtcgtgt tgaagacagt cggtttattg actttgatgg cgatgttcgg tatgcaagtt
    21781 ccggcaaaag aagggacaga gcttgctgtc tttgatgaga ttttcgtcga tgtcggtgac
    21841 caccaaaatt tagaaaatgc gttgagtaca ttttctggtc atatgcaaaa catcgctgct
    21901 attttgaaaa agataaaaag gaatacgttg gtacttttag atgagattgg cagtggaaca
    21961 gagccaaatg aaggggctgc actagcgatc gcaatcatgg aatctatgta tgagcaaggg
    22021 gcattgatca ttgccacgac ccattatgga gaaatcaaga aattcgccgg agatcatgaa
    22081 gattttgtgc cggctgctat ggctttcgat cgggaagcac ttcgtcctaa atatcagttg
    22141 cgggttggaa gaaacaggag aaagccaagc tttgtggatt gctcataaaa tggatatgtc
    22201 gatgaaattg atccatcaag cagagcgtta cttggcggaa aaagcctatc ggacacagaa
    22261 aaaagaattt cttcctaaga cagcagttac aaataggaag gagaataaaa aagaacgtca
    22321 gctctttgca aaaggtgatc ggatatttgt caatgaatac caaaaagaag cgttagttta
    22381 tgaagatata ggtgaagata cgattgatgt atatttagac aaaaaaataa tccacgtgcc
    22441 tcgtcagcgt gttcgtctgg tgcgttcagc agaagatctg tacccgacag gttacgattt
    22501 agacagtctg tttatcgatt ataaaacacg taaacggcag cgtgacctag agcgaggatc
    22561 aaaaaaagct cataaagtac tagtaaaaga aatgcgcaaa cgacaggaag aaagaagagt
    22621 aaacgatgaa aacagcaagt gattatctca aattagctca taatatcagt gtctgtaaag
    22681 aaaaataaaa acagtgaaat cattggtttt attccccgtg taagctaaaa taaaaagaaa
    22741 agagtgctga ggaggtaggg cgataaaatg aaattaagca cagactggcg taaagaaata
    22801 caaacgattc cgaatctttt atcgatattc cgtatttttt tgctgccgat ttatctttat
    22861 tttgtgctga gacagtcatt ttatatagca ggagcggtca ttgttgtctc aggtttgtct
    22921 gattatctag acggggtcat tgctcggaga tataatcagg taacagactt agggaaggtg
    22981 cttgatccgt ttgcagataa attgacacaa ctttttctga ttttgtctat ggcatggtat
    23041 cgaccgtggc tttggctgtt gtttggtcta ttcttgataa aagaaggctt tatgtttgta
    23101 gctggattaa tcgggctatc taaaaatatc aaattaagtg gagcgaagtg gtatggcaaa
    23161 gtagcgactg ctgtcatcta tgttggcatg atccttttgc tgcttttccc agaattacca
    23221 acgctgtggg tgagagtcat ttttgccgtc atcacttacg gtcttttaca atcttttgtc
    23281 ttgtatgctg tcgagtaccg aaagatgttt caaagaaaat aaaaaagggg tgtgacctat
    23341 gtcgcattcc tttttttgat aaatagactc ccagtagcta cttctcgata ataagccaac
    23401 cgaaccaaaa acaggagaag ttgtttttga aattatttct tgtgattcgg agttaaatgg
    23461 taggctcaca acctcttggt aaacggtgtt caatcagctg accttcggta ttagctcaaa
    23521 aaacggcaaa atatgagcag ctatttttcc gtttgtttct ctaatactca ggtcaaaccg
    23581 ctgattttac aacctcttcc ttaaacggta ttcaaaagcc agtttctcgg tattaagcca
    23641 aatttatgca aaaatgtaga aatccatttt cacaaaattt ttcttaatac tcgaaactaa
    23701 gcgacttttt cacaatctct tccttaaacg gtgttcaatc agctgacctt cggtattagc
    23761 tcaaaaaacg gcaaaatatg aggagctatt ttcccgtttt tttctctaat acttaggtca
    23821 aaccgctgat ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    23881 aagcccaacc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
    23941 tgcaggacag cgttttcaca acctttgatt cacggttttc cagaagtagc ttcttcgaaa
    24001 aataagccga aatttcttga agctgatcac ttctgtcgca acctctactg actaaggctt
    24061 gatataagca taaccttggg attgcagttc aactaaatca aggacacctg ctggtacaac
    24121 gattacttgt tcaggtaaag aactttctga aatatgatta gcacgcatag cattggcgca
    24181 agcgtgaaaa acgactcctt gcaaatctaa gaattctgca ttggcagagt ctagataacc
    24241 ggtaatcgcg atgccattta cagatacgac aatcgtcgtt tctggagaag cttttatcag
    24301 gttttttacg tttttccctg tttctgacca tttttctaat tcatcaatat ggaaaacgac
    24361 tttcattgaa atcattcctt tactttttaa taatcatctt gatcgcttgt tctagtttag
    24421 ccgcttgttc ttctggatta ctatcaggtt tctcaaagca atttttcaga ttatctgcaa
    24481 cgatcatgcc cattacgcga tcaatactag aacgaacagc gcttaattga gtgatgacat
    24541 ccatacattc tttttcgtct tcgatcattt tttggatacc acgaatttgt ccctctgtac
    24601 gtttcaaacg gttcaggata tttttattat caatctccat aacacattct cctctatttt
    24661 tataattaga taatacccct tatcggtatt ttatgcaaat ataaaaagta gtgaaaaaaa
    24721 tgaagagtca ttctggttga catattaata aagctcctgt ttaatatacc ccatgaggta
    24781 tttataaaaa tgtaaatgag gtgcagaaaa tgaatttgct atttcaatca ataaaatcga
    24841 taacagtccc agaattaaag gaaaagcttt tggaaaatcc agcgttactt gatgttagaa
    24901 cacctgcaga ataccggggc gggcatataa aaggagctaa aaatgttcca ttacaaagta
    24961 taaacagata tgatggagac aaagaaaaga ccgtctatgt gatttgtcaa tcagggatga
    25021 ggagtaaaca agcagctaaa gaattaaaga aatctggtta tgatgtagtg aatgtacgtg
    25081 gtggcatgaa tcaatggttt gatcgaacag taggagggaa ataagatgaa aatcgttatt
    25141 gtcggaggtg tagcaggtgg aatgtctgct gctacacggc ttcgccgatt aatggaagat
    25201 gcagaaatcg ttgtttttga aaaaggaccg tatgtttctt ttgcaaattg cggcttgcct
    25261 tattatcttt cgggagaaat cagtgaacgg gaaaatcttc ttgtccaaac gccagaatca
    25321 ttatccgctc gtttttgttt agatgtgcgt ccaaatcatg aagtgacagc catctttccc
    25381 gaaaacaaaa cggtagaagt cgtacatgag ggtcaaaaac acattgaaca gtacgatgca
    25441 ttggttttat ctcctggtgc aaaaccagtt gttccatcga ttccagggat aacagaagcc
    25501 gacaatgttt tttctattag aaatgtacca gatatcgata aagtgatgca tgcattagaa
    25561 aaacagccaa agcgtgccgt gatcgttggt gcaggattca tcggattgga aatggcagaa
    25621 aacctaaaaa gaagaggttt agaagtcatg gtgatcgaac aagcaccaca tattcttcca
    25681 acactggatg aagaaatggc agcttttata gaaaaagaat tgtctcatca aggagtagaa
    25741 gtgattactt ctcatgctgt cgctggattt gaagaccacg ggaaacgatt gcgactggat
    25801 gatgggcgta ccatccctgc tgatttagtt attttatcca ttggtgttcg tcctgataac
    25861 cagctagcag tgaccgctgg aatcgaatta ggcatacgcg ggggtatcct agtagacgaa
    25921 cgttatcaaa cgaatattcc tgatatttat gcggtggggg atgctatcgt tgtaaaacag
    25981 caaatcactg gaaaagatgc acttatttct cttgcttcac cagccaatcg tcaaggtaga
    26041 caagttgcgg acacgatttc cggaatttct cgaagaaatc aaggcggtat tggaacagca
    26101 attatacgaa cgtttggaat gactgccgca tccaccggtt taagtgaaag aacagccaaa
    26161 gaaaacgaac tgtcttttga agtcattcat gtatcaggaa aagatcatgc aagctattat
    26221 ccagaagcaa cagatatttt actgaagttg atcttccatc cagagactgg cgagatttat
    26281 ggtgcacaag gtgttggggc aaaaggtgtg gataaacgga tcgatatttt agcaacagca
    26341 atcaaagggc atttgacgat cttcgattta ccggaattag aattgacgta tgcaccgcca
    26401 tttggctcag ccaaagatcc agtaaacatg ctaggatatg cagcaatgaa cattgcagaa
    26461 gggcttagtg aaaccgtaca atggcatgaa ttgccgacag aactagcaaa aggaaaaatt
    26521 ttattagatg tgcgaacagc agaagaattg gaaaagggca aattcaagga agccaaacat
    26581 atccctttga atgaacttcg ggaccgatta aatgaattag acagccaaca agaatatatc
    26641 gtcagctgtc atagcggatt acgtagctat ctagcggaaa gaatcttgaa gcagtctggc
    26701 taccacgtaa aaaaccttga tggtgcattt tctttatatc aaactgtccg acctgaagaa
    26761 ctgatatatc ctaacaaatg atactcgtta aaaagcttgc attcgactct taaaagaggt
    26821 aaactaatcg gttccaccac tttaaaaaaa gaggagaatg tgtatggtca acacacaaaa
    26881 acagttagaa gaagcatatc tgaagaacgt gtatcaaaag ctaattgata aaaaaaacga
    26941 actgcgggaa tcgatggata cggcaaaaat agaaggaaac cagtcgttga aagaaatggc
    27001 tggcgagctt cgattgaatt ttgacagtta cttagataac ctggatactt tttcgatgat
    27061 tgaaatgaaa aatagagaaa tcgatcagat gaatatcaaa cagaaaaacg cagaaaatca
    27121 gctagcaaag atcgaacgtt tattgaaaag tccctacttt ggaaaagtcg ttgtagactt
    27181 tctggataat gagccaaaag aatcctttta tatcggtaca gcaaatttca cagatgatgc
    27241 agatgaaaat cttgtttatg attggcgttc accaattgcc gaactttttt acaataacat
    27301 gattggtcca tcttcttata cggttcgtca aaataaaatc gatacggtta ttaaagaaag
    27361 acgacaattt cttttggaaa gagatcgttt ggtcaagttt tttgatacgt ctgtagctat
    27421 tcaagatgac gtattattag aagtcttagg ccaagatgag accaatgaaa tgaaggctat
    27481 cactgctacg atccaatcgg aacaaaacac aatcattcgg gatgtgaaaa gtcaaaatat
    27541 cttggtaaat ggcgtagcag gaagtggtaa aacctctgct atcatgcagc gtattgcgta
    27601 tctgctctat ttatatcgag atacgatgac atccgatgac ttgctgattc tatcacctaa
    27661 tcatcggttt atcgactata tttcgaatgt cctaccatca ttaggtgaaa gaaatccact
    27721 gaatctaacc atcatacagc tggtttcaca gctttcagca gaggaaatag aaggtgaaga
    27781 ggcttatttc aaacgtatca cgggagaaaa tgtatcggag cagacagaac gtttgcgtag
    27841 caaaaagttt atcgataatc taaaacaatc agatcctttg tttttagatc atccgaattt
    27901 tatccgtgga cttactaaaa atggaaaaac agtcctttct aaaaaaacga tcgaaaaaat
    27961 atatgagaaa gttcctgctc atcctaaatt gatcgatagg cttcaagcaa cgaaaaaggc
    28021 actgatgagc gaatggaaga atcatctttt gaaacaagca aaaagtcctg ctgtgcagaa
    28081 tcaggtactc tcgttgacag aagataggca gttggaatta ttcggtaaat tgatttctga
    28141 tgattcggaa caaagtattg tagcttatgc aaggaaactt ttacaaaaaa aatatcgaaa
    28201 aataaccaga caaatcgaag aaatggcatg ggtggatgag catcagttgt ttgaaagaat
    28261 ttatgaaaaa cgatatggct cagcttatgc ttggcaaccc acacgtactg ttgatgaagc
    28321 agtcattata ttagctattc gtcatttact tgttgaaaag gtaaatgtac cagcatttcg
    28381 ctatctattg attgatgaag tacaagatta taccctggca caattaggtc tattgatcga
    28441 attatttcca aaaactcact ttactttagt aggtgacgag aatcaggcaa tctttaattc
    28501 ttccactact tttgctgata ttatgcgttg tttcgacgac taccatctgc cgatccatcg
    28561 gtatgatctt agaaatagtt accgttctag cggagcaatc acagagcttt tcaaaaccta
    28621 tgctgtggat caagagaaga tcgacatcgt atcgatccga ccgcaaggaa aagaacccga
    28681 ataccgctct tttcgatcct ctgaagaatt gttgaaaata ctagctgaga ttttggtttc
    28741 tctaaaagga gaaaaagcgg cagttatcac ccaaacagaa gaggaaaccc aaacgctaca
    28801 agaatcgatt aaaaaaaatt catatggatt agaaaactgc caaatcattc ctttgagtct
    28861 agcaaaagga ctggaatttg atcatgtaat cttatatcca tttgaaaacg atggagatga
    28921 gcaaagaaga cgcagacaaa tgtacacggc gatatctaga ggaatgaaaa gcattgtcgt
    28981 attggaaaga gcaacataat taagagataa aaaataaaaa gaacaactga taaaaagaac
    29041 aaaatatttt tcatttgctg atgagaaatg ttttgttctt tttcttagga aaaatttagg
    29101 aacactttat gtgaaaagaa atgaaatgta tggtaaactc ctacgtcgta acaaaaaaac
    29161 gtgaggtgag gatatgaaaa aagtaacaag agaagaagtt gcatcaattt tggtgaaaga
    29221 taagtatttt tcaaaaggta attactggtt aaagatttgg caaacgttag tggctctttt
    29281 aggatggatg atcgttgttc ttccttttat atgggtcttt ttcccattaa cgagaccaga
    29341 aagagccaag gatttttcct tgtacgcttt tttatcagaa aagaaaatgc tgtatttctt
    29401 aatcggcttt tttgtattga tttttttcag tttcctgatt acctcatttg tattgactag
    29461 gtggaacaat cgaaatctat acaaaaaagt aaataaatct tttgaatatg aagatgaaaa
    29521 attgaaaaaa agacaagaat tacttgaaga agtgtatacg gaacgttttg gaacaaaaga
    29581 agagagagaa acggtccgtt tttattctgt ctccgaagag aaaaatctag atacgacatt
    29641 cattgcagat ttgtataaag aaaatggggt ggaactgaaa tgatctcaat cacccataca
    29701 ggtaatttat tcttagatac ttgtttgtct attatgtatt tttttctcgt ttcttatccg
    29761 attttaggcg gatttgtatg gtttattggt gtctggtgtt atgtgttttt atacaaacat
    29821 aagcaaaaag aatgggtaga tgttcccttg agtgtcgaac cgttcatcac gatcatggtg
    29881 ccagcccata atgaagaaat tgtgatcgaa tatacaatcg agtatttgat gacgaaattg
    29941 aattatcaca actatgaggt attagtgaca gacgatggtt caacggacca aacaccagaa
    30001 atattagcta gactgatgaa aaaatacgct aatcttcgtg tggttcggat cgaaaaaaat
    30061 aaaggaaaag cccacgcgtt taatatcgga cttgcctttg caaaagggaa attgatttta
    30121 agcaatgatg cagacacggt accggagcca gatgcactga ttaggtatgt gaattatttc
    30181 attcgccctg gtgcaagaca tattgctgcg gtcactgcaa atatggatgt ccaaaatcgt
    30241 accaaattga ttgcaaaatc tcaaacggtt gaattctcaa gtatcgtggg catcattaaa
    30301 cgaacacagt cagctgtttt cggtggtctt tatgcgtaca gtggagcaaa tacgatgtat
    30361 cgaaaagaag ccttgatcga tgtgggcgga ttcagacaag atcgagcgac agaagacatc
    30421 agtattgctt gggatcatca gttaaatgac tggttatctg tttttgctcc tgagattatc
    30481 tttttcatgg aagtacctgt gacgttgaag atgctatatc gacacagaaa acgttgggca
    30541 aaaggtggaa cagaagtctg gctgacgaat ttcaaaaaag tgatgctcca tccttttaaa
    30601 catattggaa gaacgatcat cttcattgat caaacattga gtattgtctg gtcaatattc
    30661 ttttgtattt ccgtagttct ttttgctgga ttaatcgggc attatgtata tcaaggaaac
    30721 tacgaacaga tttatattac ttttacattc agttttgtgt tcatctgctt tgaaatggtc
    30781 gcaggatttt tccagttgct agcttcgctg attgtagatg atcgacgaag aaaattgaaa
    30841 taccttcttt tcgcaccact atatatgctt ttgttctgga tagtgaacgc gataacgatc
    30901 gtgacgactt tcattccagc agtcaaaacg attttaggtt atggaagcgg gacatggaaa
    30961 agcccggaac gtacaaaaaa ataaaagatg caccgttttt tagacaaaca gcctgttgtg
    31021 tttatggttc ttccaaacat ttttgcttat aataagaaat tagttaaaaa gtagtaagga
    31081 tgggaaaagt gatgaaaaaa agaatgaagc cgttaatctg gccaattgct gtttccagta
    31141 ttttcttatt gggacagaca gcttttgcgg aaacttctga acctgctcaa acagaacctt
    31201 caacaaccat aagctcttct ggtgctgaaa acggtgcctc ggaaacagaa atgacttcca
    31261 atgtaccgac agataattca ggagaaacga tttcatcttc agaagataca tcaataaata
    31321 gttctacctc ttctgaccaa atcgatcatt tgcaagactt ggaaaataaa agaaaagaac
    31381 tcgaagaagc attagaagct gcaaaaaaca aattgaaaga agaccaagca gcaatcgatc
    31441 aagatttagc tgatcttaaa aataaagtgg gaaatgaccg tgcacaactt gagaaaaagc
    31501 ttgccgaagc gaaagcaaaa gcggaagctg tccaaacaga tcttgacggg aagatccaac
    31561 aagcaaaaga attcaatcag caattaaatc aggttgctca aacagcttct ggaaagctaa
    31621 gtcaaactca agcggatatc acagcggcca gtcaagcaat ccacggaaag atcagcgaag
    31681 cacaaacagt tgctaatgat tttgaagcaa aaaaagctga cttagcagct agtttccaac
    31741 gaataaaaga tattttgcat tcggcaggag aaaaatcagt gacctctgga agcacgacag
    31801 aaacagttca gcaaacgaca gagccatcca aatcaacaag tcagactttg ccaaaaacaa
    31861 atgaaaaaac agcttctgtc agttattcct tagcaggggc gggtgtgtta gtcgcagcag
    31921 cttacggaat catccgcaaa aagaaataaa aaagagagga tgggaaggta gagtacctcg
    31981 aagttagact gaaaaatcta attcttgagt gccactacat gtcccatctc tctttagtta
    32041 ttaaaacaga tgtcccctac ttatctcgtg gagacagctg ttccactaca tgtgaccatc
    32101 agcatgccat tgtcgctgcc taatacttca tagtccattt ttacgccaat gatcgcatca
    32161 gctcctaatg cttttgcacg ttcggccatt tcggcaagtg cttcttctct tgcacccatt
    32221 agttcgtttt catagctttt tgagcggccg ccaaagatat ttcgcaaccc cgcctccaag
    32281 tctttaaatg catggatccc agtaatgacc tcaccaaaaa cgatcccttt ataagctact
    32341 acggcttttc cctctacact gttcgttgtt gttgtaatca ttcgatcact cctctctttt
    32401 aattaaagta taacaaactt caagaagcac tacacatatt ttcccaaaat tagaaattta
    32461 ttatatactt gaattgttca ttaatgaaag cgaaaccaat cgttagcaac tatgtaaaag
    32521 aaaggaagat tcttgtgaaa aaaagtattt tgcttatttc tcatagtcaa aaagtaacag
    32581 atggtattaa agaaatgatt gagcagatgc aaaaatccga agaggtgcaa attttttctc
    32641 tcggcggaat aaatggcgag atcggatcag acccgatgaa aatcgtagaa gcagtcaatg
    32701 aatcaagcga tgcagctagt tattttgttt ttgcggatat cggtagcgcg gtattgaact
    32761 cggaactggc aaaagacatg ttggaagaag agcagcagca acgttatcat ctggtggatg
    32821 cgcctttagt ggaaggagct tttgcagcag ccatcactgc cggtataaca gatgatactc
    32881 agcaaatctt aacagaagca caaaatgccg gaaagaaagg atgggaataa attgaaaaaa
    32941 atcatgaatg atccaagcaa tattgtcgaa gaaatgctgg aaggattagt aaaaagctat
    33001 ccagaacttg ttcatcgtgt agaatcttcc cgtgttgtag ccaagaatca gaaagcggaa
    33061 caagttggtt tggtttctgg tggaggaagc ggacatgaac catcccatgc cgggtttgta
    33121 ggggaaggga tgttaagtgc agcggtgctt ggtgatgtgt ttacttctcc aactccagat
    33181 cagattcaga cggctattaa ggaagccgat agtggaaaag gagttttgct gatcgttaag
    33241 aattatacag gggatgcatt gaacttcgat atggcaaagg aattagctgc tatggacgat
    33301 attgaagtag aaagtgtgat cgtagatgat gatatcgccg ttgaaaatag tacgtatact
    33361 gctggaaaac gcggagttgc aggtactgtc cttgtacaca aaatcgtggg cgatgcagca
    33421 agaaacggtg cttccctagc tgagttgaaa gaactgggag aaaaggtcgt acaggcaact
    33481 aagacgatcg gtctagcgct tcgagcagca actgtaccag aagtagggaa accaggattc
    33541 gaactaggag aagatgagat cgagtatggt gttggtatcc acggtgagcc aggctatcgt
    33601 cgagaaaaga tgcagccttc taaagtactg gcaaaggaac ttgttactaa gattttagat
    33661 gattactcga atttaccaaa agaagcaggt gtactagtca acggaatggg cgggacgcct
    33721 ttgatggaac agttcgtgtt catgaatgat gttttagctc tgttggaaga aagaggcgtc
    33781 aatgtagttt tccgaaaagt cggtaatttt atgacctcac tagatatgca aggtctttct
    33841 ctaacactga tcgacttgac ggagacacaa tggaaagatt ctttagaaag caatgtacag
    33901 acgatttcat ggtaaaaaat ctcaagataa aagggtgata atatgaaact aaccgttcaa
    33961 gaaatccaag aatggctcac gcaatttgca gaggcaatca atcagaacaa acaatatctc
    34021 agtgatctag ataccccgat tggcgatgga gaccatggaa ataacatggg aagaggggtt
    34081 tcagcttatg aagaagcttt tcaagccgat catcctgaaa caatcagcga tacattcaaa
    34141 gtattctcta tggcgatgat atcgaaagtc ggtggggctt ctggtccgtt atacggatct
    34201 gcattcatga acatgatgaa agcgacaaaa gaggtcgatt ctattgattc tcaagaaaaa
    34261 ttaggcgaag tgatcgagca gggaacagcg ggtattcaaa gccgaggaaa ggcagaagct
    34321 gacgataaaa cgatgttaga tgtttggctg ccagtcacag aggcattgaa ggcagggaat
    34381 ttaacaaaag aaatcattga ccaggcaaaa gaacacacca aagtcttggt agctaaaaaa
    34441 ggacgagctt cctatctagg agagcgtgca atcggacata tcgacccagg agctgcttct
    34501 agtgccattt tattttcaac gcttttagat gtgatcaatc aaccttaagt tcaataaaac
    34561 tgcggtgatg ctcatcgcag ttttttgttt ggctgttctt atgacttgat gcagaacagc
    34621 tttttcacaa cctctatttg tccatatcgc aaaattcagg tagaatacaa aagataacgg
    34681 aaagaggcga aacaatggaa cttggaaaat cagattatca gttgtttgat cgaccaatct
    34741 acgcattcaa acagctcaaa gagagccatc caacagataa gattgaacaa atcaagaaag
    34801 aatataaaga acactggcaa aaatggaaag agattcagct ccagacagct gcacttttgc
    34861 ctgatatgta cggcatgagc aaacctaaaa tcgaaagctg gacgaatggc tggaatttac
    34921 gcagtcattt ttggagcgcg taccgaagcg aatctcgtca agacgagaat gcctgtctag
    34981 cagttttgtt gaatcaaaaa caataccaaa tctatttgat gtaccaacat tataaaagcg
    35041 aagaacgata tggtagcatt gcagcgtata accagttgct tgcgatactg aaagagtgga
    35101 gcaaaacagt cgatatcaag gattattaca tatggccgca accagaacat gaacttgacg
    35161 accacttagc tttgtctgac tatttgtccg atacaaagaa acaagaagag ttgaaaaaag
    35221 cgatgagaga ccgaaccttt caaatgggta aactattctt ctatccgcaa gaaatcgaac
    35281 acgttgagca aataaccgct gaaaccttac aagaactagc accactatat caaaagctag
    35341 gtgcggaaaa atttcaatag acaacacgaa ataagcaatg cgaaaaacaa accttactct
    35401 cggcttgcga gtgaaggttt gtttttgatt attcaaaaag taaatattta aaaaattgat
    35461 aggaatcaat tacttaaggt ctcaaaagaa ggaaaataaa gcaagggaca aaaagaaatc
    35521 cactctattt gctggtatct ggttcgcttg acgtttatgc tatgataaaa gaaaattatc
    35581 agttggaata agggaagtta tgtttcttta ttagaaatag ctgtttagaa ggaggagaga
    35641 ttatgagtca tgatcattta tgtgtttcat ttgttccttt gttcaatcac ttagtacagc
    35701 aagatcaaga aaaagtacac gcacttgtca aacatcacta tgtggaaaaa ggagaacaga
    35761 ttcttgcgcc gaatggagaa cctcaattag tgatcgtggc ctccggcagt atgaaagtgt
    35821 atcaattatc tgctagcgga aaggaacagc ttttacgagt aatagagccg ggcggatatg
    35881 agggagagag ccagttgcta ggcgttccaa atgagtcttt atttggcgaa gcactagaaa
    35941 aaacggaaat ttgcatattg agacaaaaag atttctcgga actcttactg cattatcccg
    36001 cccttagtct gaaactttta gcaatcaacg ttgagaaatt aacaaaagta cagcagcaga
    36061 caactttttt gatgatggaa aaagtagaag aacgagtcgc tgtctatctg cttgacttag
    36121 ctaaagcgac agggaaggat cactttttcc tgccaatgaa aatgaaagaa cttgctgcat
    36181 ttatcggtac gacccccgaa actttatcta gaaaatttaa attattagag gagaatggtt
    36241 atgtaaaaag aaagaatcga gaagtctgga tcattgataa agagcagtta gaagacttgt
    36301 aaataaaaaa atgacctcca aatgttggat ttttgaccca gatttggagg ctagcatgaa
    36361 attgtacatg cttttttatt atccttctag taagtctctt cgcctataac caatagaacc
    36421 aaggacgaac agtgcaaggc tgatacatga taacgtaagg aacacggaaa gatcaaaaga
    36481 atcgtttggc atccgtggaa cccagctagg gacagctgtt ttggaaaacc attctggtaa
    36541 atccaaaatt ccgccaaagt aattgagtgc aaaggagtaa gctaagtaga tataagcaag
    36601 tttaccgatt cgaggaagcc atccgaatag aaagccacta attccgataa acactaaaac
    36661 ggcaggcagg aaattgaacc cggctttaag aaacatcaat agatccatgt ctttcgcatc
    36721 cttcatcgaa gccaaagcag aaagccctag tgcaccagaa ctaatcagga ttgccaatat
    36781 agcaaataga atagctagta acatattggt ccaataaagt tggttcctag aaatttttgt
    36841 tgcgaacagc tgattcatac gtccgtgggt ttcttctgta aataaacgat tgatgctagc
    36901 aattggtaaa atagcagcta gacaaatcaa gactaccatg atcgtgcttg taaagttttt
    36961 ttcaattgag acgccagatt ggataaacat ctgtttcatt aagtcgttac tttctaaaaa
    37021 ctgttgcata ttcccgtaga tagagccgta tgccgccccc atcaaaccaa aagtgataag
    37081 ccaactgatt gtcatccctt tatttagata gaataataaa ccacgcaagg ataaaagaga
    37141 acgtttagca tacggttttc cttcacgttc ggggatatag ccggtgttca aatctcgatg
    37201 ttcctctaaa acaaatgaac tgattatcaa gattagactg aacaaaaaca aataaagcaa
    37261 aggaatccag ttgttagttg taaatggata ggtcaaataa gaccagccta aaggattcca
    37321 catcgaaagg gaggaatcag ctacatctgt tattgctcgt agaaggtaaa gtccacctaa
    37381 aaacgctaaa gacagaccat tcgaactggc tgaacttggc atcagttgtg cagcaaacag
    37441 tgcaacggca ctccccaaaa tccccatact acctacagat agcccaaaaa gccaagcacc
    37501 ttctgcagtg atcgaatccg cttggaaaaa aatcatcgta cagctgatcg atattgctaa
    37561 aagcaaattg atcagaatac tttcagcgat gacagctaaa gagtttgctt gacgtccgac
    37621 tcgaaaggaa cgaataagtt caatcagacc aagttcttct ttttttctcg tttggctcac
    37681 cacgtgtaaa acagagatca cagcagcaaa caaactgcaa aaaagcaaca tttcatttgc
    37741 atacatcgct cctaaagtat actcagcagc attcgaagct ggagttggtc cgaccattgc
    37801 aatcattgcc gggttttgca aggtttcgta taatccagct aatccttgtc cggaagtcaa
    37861 ttcttcaaac gcagggacaa aaccaccaga aaaagcagtg acaccgacta gccatatcag
    37921 gatctttttc cagtcacggc taaggttttg agaaaaaagg gtggaccagc gtgaaaattt
    37981 atcagtcatt tcgcttcctc ctcatccttc ataatgtctc atgaataaat cctcaagagt
    38041 cggtggaata gattcaaatt ttttcacacc taatttggac gcttcagcta aaatcacatc
    38101 gaaagcttcg ttatcagcag caaattgagc gaggtgacct ttttgcacaa agtcatggac
    38161 accgttcaat tgtgctaagt gactattctc tgcttctgtt tctatagtga tggtcgaacg
    38221 agtcaaatga cgcaattcat cgagtgttcc ttcttcatcg agtgttcctt cgcgtatgat
    38281 cacgacttta tccgctagtc gctcgacttc actgagaata tgagaagaaa gtaggatgga
    38341 tttgccctgt tctttcaatt tttcaacttc ttcttggaaa atcgtttcca tcaaaggatc
    38401 taaaccagaa gtaggctcat caaataaata gagatccgaa tcaacagcca aagcagcaat
    38461 caagcccact ttttggcgat ttccttttga atagtttttt gcttttttct taggatcaag
    38521 agagaaacgt tgaatcagtt catctttttt ttgagcactc ccttttccat gaagcttcat
    38581 gaagagatcg atgatcgctc ctccagtcaa atttccccac aaagagatat cgcctggaac
    38641 ataagaaaga cgttcatgga tagataaact atcttcccat acatcttgcc caaaaatcgt
    38701 tgccgttccg ccgtcttttt ttataatccc taaaagtgtc cgaatcgtcg tggattttcc
    38761 agaaccattt ggtccgataa atccaacgac ttcacctgaa tgtacggtga aagagacatc
    38821 ttttaaagct tcgaaacgac caaagctctt tttcaaatgt gtgactttga cgatttctgc
    38881 cataaaaacc ccactccttt ttctaaaaat atatactaaa caagattaaa tagtttaata
    38941 aaaaggtttt ataccttttt ataaaacttt ttttattatt ttagtacata atattctttt
    39001 tataaatttc tgtcaatgaa ttcataaaca aggaattgct agcttgat
//
LOCUS       DAZKNM010000010        34815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000010 DAZKNM010000000
VERSION     DAZKNM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.010"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8M9_000420"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8M9_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0377219.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG8M9_000421"
     CDS             complement(2020..2922)
                     /locus_tag="ACG8M9_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0377220.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG8M9_000422"
     CDS             complement(2919..3611)
                     /locus_tag="ACG8M9_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0377221.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG8M9_000423"
     CDS             complement(3654..4295)
                     /locus_tag="ACG8M9_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0377222.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG8M9_000424"
     CDS             complement(4333..4647)
                     /locus_tag="ACG8M9_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377223.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8M9_000425"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8M9_000425"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0377224.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8M9_000426"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8M9_000426"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0377225.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG8M9_000427"
     CDS             complement(5984..6916)
                     /locus_tag="ACG8M9_000427"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0377226.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG8M9_000428"
     CDS             complement(6913..7689)
                     /locus_tag="ACG8M9_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0377227.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8M9_000429"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8M9_000429"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0377228.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG8M9_000430"
     CDS             complement(10933..12009)
                     /locus_tag="ACG8M9_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0377229.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG8M9_000431"
     CDS             complement(12014..13294)
                     /locus_tag="ACG8M9_000431"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0377230.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG8M9_000432"
     CDS             complement(13313..14239)
                     /locus_tag="ACG8M9_000432"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0377231.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG8M9_000433"
     CDS             complement(14252..15535)
                     /locus_tag="ACG8M9_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0377232.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8M9_000434"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8M9_000434"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0377233.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG8M9_000435"
     CDS             complement(16323..17234)
                     /locus_tag="ACG8M9_000435"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0377234.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8M9_000436"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8M9_000436"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0377235.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG8M9_000437"
     CDS             complement(17700..18398)
                     /locus_tag="ACG8M9_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0377236.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG8M9_000438"
     CDS             complement(18385..18771)
                     /locus_tag="ACG8M9_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377237.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG8M9_000439"
     CDS             complement(18918..20588)
                     /locus_tag="ACG8M9_000439"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0377238.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG8M9_000440"
     CDS             20771..21004
                     /locus_tag="ACG8M9_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0377239.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG8M9_000441"
     CDS             complement(21103..21534)
                     /locus_tag="ACG8M9_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0377240.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACG8M9_000442"
     CDS             21613..22026
                     /locus_tag="ACG8M9_000442"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0377241.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACG8M9_000443"
     CDS             complement(22064..23212)
                     /locus_tag="ACG8M9_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0377242.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACG8M9_000444"
     CDS             complement(23334..23930)
                     /locus_tag="ACG8M9_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0377243.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG8M9_000445"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG8M9_000445"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0377244.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACG8M9_000446"
     CDS             27160..27591
                     /locus_tag="ACG8M9_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0377245.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACG8M9_000447"
     CDS             complement(27636..28985)
                     /locus_tag="ACG8M9_000447"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0377246.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACG8M9_000448"
     CDS             complement(29055..30218)
                     /locus_tag="ACG8M9_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0377247.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACG8M9_000449"
     CDS             30347..30670
                     /locus_tag="ACG8M9_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377248.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACG8M9_000450"
     CDS             complement(30853..32202)
                     /locus_tag="ACG8M9_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0377249.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACG8M9_000451"
     CDS             complement(32271..33224)
                     /locus_tag="ACG8M9_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0377250.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACG8M9_000452"
     CDS             complement(33326..34516)
                     /locus_tag="ACG8M9_000452"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0377251.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9901 a         7617 c         6104 g        11193 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgca
//
LOCUS       DAZKNM010000011        34544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000011 DAZKNM010000000
VERSION     DAZKNM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.011"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..835)
                     /locus_tag="ACG8M9_000453"
     CDS             complement(<1..835)
                     /locus_tag="ACG8M9_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0377252.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YF"
     gene            complement(1059..2999)
                     /gene="thrS"
                     /locus_tag="ACG8M9_000454"
     CDS             complement(1059..2999)
                     /gene="thrS"
                     /locus_tag="ACG8M9_000454"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0377253.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3336..3515)
                     /locus_tag="ACG8M9_000455"
     CDS             complement(3336..3515)
                     /locus_tag="ACG8M9_000455"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0377254.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3562..5385)
                     /locus_tag="ACG8M9_000456"
     CDS             complement(3562..5385)
                     /locus_tag="ACG8M9_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0377255.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5663..7942
                     /gene="helD"
                     /locus_tag="ACG8M9_000457"
     CDS             5663..7942
                     /gene="helD"
                     /locus_tag="ACG8M9_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0377256.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8035..8682)
                     /locus_tag="ACG8M9_000458"
     CDS             complement(8035..8682)
                     /locus_tag="ACG8M9_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377257.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8861..9580)
                     /locus_tag="ACG8M9_000459"
     CDS             complement(8861..9580)
                     /locus_tag="ACG8M9_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0377258.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9711..10862)
                     /locus_tag="ACG8M9_000460"
     CDS             complement(9711..10862)
                     /locus_tag="ACG8M9_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0377259.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11079..11819)
                     /locus_tag="ACG8M9_000461"
     CDS             complement(11079..11819)
                     /locus_tag="ACG8M9_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0377260.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11823..12146)
                     /gene="rsfS"
                     /locus_tag="ACG8M9_000462"
     CDS             complement(11823..12146)
                     /gene="rsfS"
                     /locus_tag="ACG8M9_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0377261.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12203..12796)
                     /gene="yqeK"
                     /locus_tag="ACG8M9_000463"
     CDS             complement(12203..12796)
                     /gene="yqeK"
                     /locus_tag="ACG8M9_000463"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0377262.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12780..13430)
                     /locus_tag="ACG8M9_000464"
     CDS             complement(12780..13430)
                     /locus_tag="ACG8M9_000464"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0377263.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13440..13751)
                     /gene="yhbY"
                     /locus_tag="ACG8M9_000465"
     CDS             complement(13440..13751)
                     /gene="yhbY"
                     /locus_tag="ACG8M9_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0377264.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13767..14876)
                     /gene="yqeH"
                     /locus_tag="ACG8M9_000466"
     CDS             complement(13767..14876)
                     /gene="yqeH"
                     /locus_tag="ACG8M9_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0377265.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14873..15400)
                     /locus_tag="ACG8M9_000467"
     CDS             complement(14873..15400)
                     /locus_tag="ACG8M9_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0377266.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15580..16365)
                     /locus_tag="ACG8M9_000468"
     CDS             complement(15580..16365)
                     /locus_tag="ACG8M9_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0377267.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16358..17227)
                     /gene="accD"
                     /locus_tag="ACG8M9_000469"
     CDS             complement(16358..17227)
                     /gene="accD"
                     /locus_tag="ACG8M9_000469"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0377268.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17228..18607)
                     /locus_tag="ACG8M9_000470"
     CDS             complement(17228..18607)
                     /locus_tag="ACG8M9_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0377269.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18615..19034)
                     /gene="fabZ"
                     /locus_tag="ACG8M9_000471"
     CDS             complement(18615..19034)
                     /gene="fabZ"
                     /locus_tag="ACG8M9_000471"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0377270.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19059..19535)
                     /gene="accB"
                     /locus_tag="ACG8M9_000472"
     CDS             complement(19059..19535)
                     /gene="accB"
                     /locus_tag="ACG8M9_000472"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0377271.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19538..20773)
                     /gene="fabF"
                     /locus_tag="ACG8M9_000473"
     CDS             complement(19538..20773)
                     /gene="fabF"
                     /locus_tag="ACG8M9_000473"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0377272.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20792..21529)
                     /gene="fabG"
                     /locus_tag="ACG8M9_000474"
     CDS             complement(20792..21529)
                     /gene="fabG"
                     /locus_tag="ACG8M9_000474"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0377273.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21541..22458)
                     /gene="fabD"
                     /locus_tag="ACG8M9_000475"
     CDS             complement(21541..22458)
                     /gene="fabD"
                     /locus_tag="ACG8M9_000475"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0377274.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22565..22789)
                     /locus_tag="ACG8M9_000476"
     CDS             complement(22565..22789)
                     /locus_tag="ACG8M9_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0377275.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22842..23804)
                     /locus_tag="ACG8M9_000477"
     CDS             complement(22842..23804)
                     /locus_tag="ACG8M9_000477"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0377276.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23814..24254)
                     /locus_tag="ACG8M9_000478"
     CDS             complement(23814..24254)
                     /locus_tag="ACG8M9_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0377277.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24560..25525
                     /locus_tag="ACG8M9_000479"
     CDS             24560..25525
                     /locus_tag="ACG8M9_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0377278.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25582..25812)
                     /locus_tag="ACG8M9_000480"
     CDS             complement(25582..25812)
                     /locus_tag="ACG8M9_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0377279.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25898..26785)
                     /locus_tag="ACG8M9_000481"
     CDS             complement(25898..26785)
                     /locus_tag="ACG8M9_000481"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0377280.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26874..28097)
                     /locus_tag="ACG8M9_000482"
     CDS             complement(26874..28097)
                     /locus_tag="ACG8M9_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0377281.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28114..29160)
                     /locus_tag="ACG8M9_000483"
     CDS             complement(28114..29160)
                     /locus_tag="ACG8M9_000483"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0377282.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29324..31051)
                     /gene="ptsP"
                     /locus_tag="ACG8M9_000484"
     CDS             complement(29324..31051)
                     /gene="ptsP"
                     /locus_tag="ACG8M9_000484"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0377283.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31051..31317)
                     /locus_tag="ACG8M9_000485"
     CDS             complement(31051..31317)
                     /locus_tag="ACG8M9_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0377284.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31796..34030
                     /locus_tag="ACG8M9_000486"
     CDS             31796..34030
                     /locus_tag="ACG8M9_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0377285.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34178..34498
                     /locus_tag="ACG8M9_000487"
     CDS             34178..34498
                     /locus_tag="ACG8M9_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0377286.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9750 a         7479 c         6035 g        11280 t
ORIGIN      
        1 taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt
       61 tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag
      121 ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta
      181 tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt
      241 tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa
      301 gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa
      361 aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag
      421 aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt
      481 ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac
      541 caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta aaaccttcat
      601 cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac
      661 cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt
      721 cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt
      781 tttctgctga agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt
      841 cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc
      901 gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc
      961 ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt
     1021 gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt
     1081 ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta
     1141 cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat
     1201 tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga
     1261 tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa
     1321 tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt
     1381 ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca
     1441 attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc
     1501 aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta
     1561 ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct
     1621 gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt
     1681 tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag
     1741 cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta
     1801 cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc
     1861 atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt
     1921 cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc
     1981 atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc
     2041 attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca
     2101 acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg
     2161 atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca
     2221 gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct
     2281 ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag
     2341 gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct
     2401 gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga
     2461 cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca
     2521 ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa
     2581 agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt
     2641 gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct
     2701 gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg
     2761 gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg
     2821 atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc
     2881 actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat
     2941 gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata
     3001 atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat
     3061 cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca
     3121 aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt
     3181 tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct
     3241 tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc
     3301 actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca
     3361 ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc
     3421 gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg
     3481 cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt
     3541 tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat
     3601 ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg
     3661 caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat
     3721 acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat
     3781 cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc
     3841 actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc
     3901 tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact
     3961 ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac
     4021 tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt
     4081 cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga
     4141 aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt
     4201 ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac
     4261 tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct
     4321 gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact
     4381 agctgcatac agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac
     4441 ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc
     4501 gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat
     4561 tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag
     4621 tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata
     4681 agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt
     4741 ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt
     4801 ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc
     4861 aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc
     4921 tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc
     4981 atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc
     5041 aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta
     5101 atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc
     5161 tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc
     5221 tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa
     5281 tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat
     5341 cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct
     5401 tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata
     5461 gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag
     5521 cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat
     5581 tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta
     5641 cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga
     5701 aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac
     5761 tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa
     5821 tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa
     5881 ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag
     5941 tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg
     6001 tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat
     6061 tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag
     6121 aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc
     6181 tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc
     6241 atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat
     6301 tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc
     6361 cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga
     6421 aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc
     6481 ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct
     6541 gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa
     6601 agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat
     6661 ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat
     6721 cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca
     6781 tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga
     6841 cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc
     6901 aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc
     6961 caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt
     7021 tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac
     7081 acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt
     7141 gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac
     7201 atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt
     7261 cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg
     7321 tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct
     7381 tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga
     7441 aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag
     7501 aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt
     7561 agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc
     7621 taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca
     7681 agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc
     7741 ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca
     7801 tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga
     7861 attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc
     7921 ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt tagcagttgt
     7981 ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg tcatttattc
     8041 tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt
     8101 cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc
     8161 gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa
     8221 attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc
     8281 actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt
     8341 ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc
     8401 ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt
     8461 ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata
     8521 ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg
     8581 cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct
     8641 taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct
     8701 tccacatact tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat
     8761 tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct
     8821 tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat
     8881 tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg
     8941 tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga
     9001 ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta
     9061 cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt
     9121 tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg
     9181 tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa
     9241 atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca
     9301 ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg
     9361 caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag
     9421 cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg
     9481 ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag
     9541 cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga
     9601 tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg
     9661 tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg
     9721 attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca
     9781 cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa
     9841 atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga
     9901 taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa
     9961 gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc
    10021 tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc
    10081 tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc
    10141 caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc
    10201 acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct
    10261 atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt
    10321 aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga
    10381 tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt
    10441 ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct
    10501 tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt
    10561 ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa
    10621 tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt
    10681 tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa
    10741 aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc
    10801 acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc
    10861 atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca
    10921 aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat acttctgcga
    10981 cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag
    11041 atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag
    11101 aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact
    11161 ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct
    11221 tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag
    11281 gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca
    11341 aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg
    11401 gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac
    11461 acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt
    11521 gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat
    11581 gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac
    11641 cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc
    11701 agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc
    11761 tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata
    11821 tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag tttttccaga
    11881 ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc
    11941 aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct
    12001 tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag
    12061 atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct
    12121 gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg
    12181 atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg
    12241 tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa
    12301 atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa
    12361 aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct
    12421 tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta
    12481 attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca
    12541 ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt
    12601 cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt
    12661 ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc
    12721 tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac
    12781 tatagtccat attcatacaa cccttcattt tgaatataat caattacttt atctggaaca
    12841 aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg
    12901 atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga
    12961 cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa
    13021 tattctacca tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc
    13081 aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca
    13141 atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca
    13201 tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg
    13261 tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga
    13321 ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct
    13381 tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct
    13441 tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat
    13501 aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact
    13561 tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct
    13621 cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg
    13681 atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct
    13741 cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca
    13801 ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg
    13861 gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat
    13921 gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat
    13981 tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac ataagcaata
    14041 aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt
    14101 tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc
    14161 aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca
    14221 atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac
    14281 tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc
    14341 gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct
    14401 tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca
    14461 tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt
    14521 tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca
    14581 ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca
    14641 ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc
    14701 gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag
    14761 acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg
    14821 ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga
    14881 ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt
    14941 cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga
    15001 tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca
    15061 ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta
    15121 aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac
    15181 gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa
    15241 tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta
    15301 aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga
    15361 tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa
    15421 aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa
    15481 aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa
    15541 taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt
    15601 gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat
    15661 caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt
    15721 ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat
    15781 caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc
    15841 tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag
    15901 agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa
    15961 atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc
    16021 tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc
    16081 cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt
    16141 tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt
    16201 gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc
    16261 atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc
    16321 tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt
    16381 tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa
    16441 tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt
    16501 cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt
    16561 ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt
    16621 ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga
    16681 tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg
    16741 tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt
    16801 ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact ccaataatga
    16861 agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat
    16921 cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa
    16981 gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg
    17041 caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg
    17101 tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg
    17161 agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca
    17221 gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt
    17281 gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct
    17341 gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct
    17401 tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat
    17461 aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac
    17521 ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat
    17581 tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca
    17641 cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga
    17701 ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat
    17761 cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag
    17821 ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag
    17881 gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat
    17941 gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa
    18001 tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg
    18061 aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac
    18121 cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt
    18181 cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca
    18241 tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa
    18301 taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac
    18361 caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa
    18421 cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact
    18481 gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt
    18541 cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag
    18601 aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg
    18661 tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact
    18721 tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg
    18781 ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct
    18841 tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct
    18901 tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc
    18961 atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt
    19021 tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag
    19081 agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg
    19141 tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct
    19201 cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga
    19261 taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt
    19321 tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa
    19381 actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc
    19441 atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat
    19501 tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc
    19561 acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg
    19621 caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat
    19681 tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc
    19741 cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc
    19801 tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg
    19861 tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca
    19921 tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt
    19981 aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt
    20041 gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat
    20101 tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg
    20161 ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa
    20221 gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc
    20281 cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa
    20341 tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg
    20401 cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag
    20461 atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct
    20521 ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat
    20581 cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc
    20641 ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc
    20701 tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta
    20761 ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga
    20821 cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa
    20881 tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt
    20941 tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg
    21001 taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt
    21061 tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat
    21121 taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga
    21181 aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca
    21241 caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt
    21301 cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc
    21361 ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc
    21421 gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga
    21481 ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc
    21541 ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat
    21601 tgttttatcg atttttttca taaatccgct taatactttt ccggggccga cttcgatgac
    21661 ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat
    21721 gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt
    21781 gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt
    21841 ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat
    21901 tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc
    21961 accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct
    22021 agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt
    22081 cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac
    22141 tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag
    22201 tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct
    22261 gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa
    22321 gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac
    22381 aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa
    22441 taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct
    22501 attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta
    22561 atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt
    22621 cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat
    22681 ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt
    22741 cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt
    22801 cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata
    22861 cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag
    22921 gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta
    22981 ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca
    23041 attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct
    23101 ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg
    23161 ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt
    23221 tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc
    23281 agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac
    23341 acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc
    23401 agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat
    23461 cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt
    23521 acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa
    23581 tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat
    23641 aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa
    23701 atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca
    23761 ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta
    23821 tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc
    23881 gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga
    23941 aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg
    24001 cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca
    24061 acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt
    24121 gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca
    24181 gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt
    24241 aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc ttttgatgtt
    24301 caaactatat cttaactttt ttcaaaatgc aaccttaaaa gatcattttt ttactatatg
    24361 agcttttttt aagattttac tcatttaaaa caactgataa atcagcattt ataatttctt
    24421 ttattagaaa aatcatctat ctcacatatt ttgattatca aagtattttc tgtgaacaga
    24481 actatagaat tccctaaaac ttgtccatta tttcaaaatt tgttatagtc tttttattca
    24541 aagcgtaaaa ggagatccta tgttaaaaga aaaaatgaaa actctcccca tgctgccggg
    24601 tgtctatctt atgaaagacc gagatggtaa ggtcatctat gttggtaaag caaaacggct
    24661 caaaaataga gtaagcagct attttcatca gaataaacag cattcaaaaa aagtattacg
    24721 tatgattcat catataacag attttgattt cgtcgtagta gacacggaat tagatgcttt
    24781 attgctcgag tgtcaattga tccaacatta ccgtcctttt tataaccgac aaatgaatta
    24841 tttcagtaat tataattatg tacatattac caataaggga tttgtattaa cagatactcc
    24901 tacggctcga acatacgggc cttttcgtct ctataaaaaa atgccgtcta ttttgcgtat
    24961 aatggaagaa acttatcaaa tgccttggtt gtctgaaatt agtttgttag ctttgcgagt
    25021 ccagcttcct gacctacagg aaatgacttt tgaacagaaa aaaaaggaac tccaagggct
    25081 gtttcaaggc cgaaataaaa agctactgac ttacttgaaa aaaagacaac aacatttcat
    25141 ctatcagttg aatttcgaaa aagccggtat gcttcaaaaa gatatcgaat tagttactta
    25201 ttttattaga cggatccagg aacaaaagca gtttttaaga acaccatcgc tcaccttcag
    25261 catgccatta gcagcagatg agagtcagaa aaagcactac ctcgtttgtt atggtcaact
    25321 tgcagaaaca atgattacct ctggtgacgt ttctcctgac ttctattatg aaaagaaaga
    25381 agctcattta tctttgaaac gtcaattgtc aaaagaagaa atcgatcctg tacaaatctt
    25441 gatcagttac cgcaaaaaat tagaaaaaga acagtttgaa attgaactgt taaacaaaaa
    25501 agaagctgaa aagcaactaa attagttgct tctcggcttc ttttaggcat cttcaacttc
    25561 aaatgtcaat tatcctaaaa tttattggat taagtcatag atttccatcg ctacgatatc
    25621 gatattatca aattcgtatt gctgatgagt atctgtttca gttaattcaa atgtatcggt
    25681 cgctttatca taagttactg tacacttctc tacgccttct ttttcaaaac ggcgaacttg
    25741 cagttcatta tctgctgact cgaccatagc ctccagtcgt tttatgattg cgactaattg
    25801 tgaatgtgtc atcggaggtc ctccttttct tatttctgtt atattgtatc aaaagcatag
    25861 cctacttgca ttgattttct ctaaaaaaac tcgtttttta tgattttttt tctggtattt
    25921 ttccttcagg ccaccaaagt ttgatcaaag cttgggttcc gtcattctca aaacctttat
    25981 ctgccaactc ttcatatagt ttcaatgctt gggtagttgc tggcaataca agatccattt
    26041 tttctgcttc ttctaatgca atctttaaat ctttgataaa atgtttgaca aaaaaaccag
    26101 gtgtgtaatc ttcttttaaa atacgaggag aataattgct taaagaccag ttagctgcac
    26161 ttccacctga aagcgtctca atcactttct ttaaatcaag tccgtttttt tgtgcataga
    26221 caagcatttc cgtcattcct gtcatcgtac ctgcaatcat gatctgatta gccatttttg
    26281 tatgctgacc tttcccagca gaaccatgca atgtaaatgt cgtaccaaat tctttgaata
    26341 aaggcagtac ttgatcataa acggcttctt ctccaccgac catgatcgtc agtgtaccat
    26401 tctttgctcc taagtcacca ccggataccg gtgcatctaa cgcatctgcg cctttttctt
    26461 ttgctgtttt tgcaattttt tctgccaggc ttggtgtact tgtagtcaaa tcaactaaaa
    26521 tatgtcctga gatatctgca gaaaaaatcc ccgactgtcc atagtagatt tcttctacat
    26581 ctttggggta acctaccatt gtaaagagaa tattactttg ttctgctacc gcttttggtg
    26641 tatctgccca gatagctcct tctgttacta aaggatcagc ttttgctttt gtccgattat
    26701 agacagctac ttcgtgtcct gcttctagta gatggcgtgc gacagcactt cccatgacgc
    26761 ctgttccgat aaaaccaagt ttcatttttt cgacctccta aaaatcaaaa atccttgctt
    26821 ctatgctagt atacgctaga aacaaggatt ttttcataaa aaatgtttga attttatctt
    26881 ttaaatgaat ttaattttat tttgattcgt tcaatcgagt cttcttcttc ttttcttcgc
    26941 atctcttctt catgataaac aaccatgtct tgataaaatt cgatcatagc ttcgccgaaa
    27001 tgtgtagatg aaatatcata aagtttagct tctaaaactt ttggatcttt aggaacttca
    27061 ctggaaatat aatcaataag tgctggtgca aagtcttcgt cggtttcaaa agttttccct
    27121 aatgaaggat gatcgaacaa tcggttcaaa taagcatttc cttccacgac acaaggtgtt
    27181 ccggctgcca tcgcttctgt ataagtaagc ccttgtgttt ctgaagtaga agcactgaca
    27241 aaataatctg ccgctttata ataaagtgct acatcattgt tgtccacttc tcctgtgaac
    27301 tgcacaaatt cttctaactg cagttctgag atcaattcct ctaactcttc tacataaggt
    27361 ccttttccta cgataactaa tctggcgtta ggataagcgg agatgacctg aggaaaccca
    27421 ttaatcaagg cttggatatt tttctcataa gaaatcctgc ttaaagaaag aatcatcaaa
    27481 tggttttcct gaagacctag tgactccctt aatttgctga tatcctgctg agtgatatca
    27541 gaacgtttga atttttcaat gtcgatacca gtaggaatca cgcgaagcgg tgctattaca
    27601 ccatagctgc gtaatgtatc aataacacgt tcacttggac aaactacacc tgtcgtatga
    27661 tttgcaaata atctagacaa gtattttaca tgggattgcc tgatcacttt tcctttaccg
    27721 atataatgaa ggtagtcttc atacatcgta tggtaagtat ggatcagagg tattttcaac
    27781 ttcttagcta ccatcttccc aagtatccct gctccgaatt ctgtatgggt atgaataaga
    27841 tctagttcta gttctttggc aaccaaataa gcataccaca tgcctctaac aacaactctt
    27901 cgatccttga aagaaataaa tggtacactt ggcattcgaa tcacatcttt ttccaactta
    27961 tccgcattag gatcggtcgt tgtaaaaata tagacttcat gcccatgtct ttctaattct
    28021 tgttttaatg ttttgatcga tgtagcaact ccgctgactt gcggaaagta ggtatcggta
    28081 aaaaagccga ttttcatgtt tttcgcctcc aaactagtcc ttgacctgat ttactttttt
    28141 taatttgcta gagacttttt cattcagtac actttcgtat acatttttaa gttcttcacc
    28201 gattttattg atacttcgct cttttgctgt ttgataaccg gcttgtgtcg tactcggtag
    28261 ttcttgattg gtaatctgtt ggatcagtcg gataaattct tcgtttgtct tccccatata
    28321 gcagttcttg ctatcttcta accagccatt atacactgga atatctcgaa caagtacgtt
    28381 ttgtttgctt gctaaagctt ctaaaacaac gatcccttct gtctcttcat aagaagggaa
    28441 aaagaataga tctgcacctg aatacgcacc ttctatgatc tctccacgaa tgtaaccagg
    28501 aaacaggaca ttttcaggat gatcttcttt tacaattttc cggatgttcc gaggaatcga
    28561 gtacatggga acatgaccaa accaaataaa tgtgtattcg ggcatcttct tagcaatttc
    28621 tacgaaatca atgattcctt tacgttcgaa aaacaaaccg acacaaatga ttactttttg
    28681 atccggagac agtttgaaat attctctgaa cttttgttct ttcattggat cagggtgata
    28741 tttctctaag tcgatcccat tggaaatagc ttgaatcggc acacggatac catagctttc
    28801 caacaactgt tttgaataag gtgttggcgt gatcagatag tccgcttttt ggtacaatcc
    28861 taccagatat ttttttacga taggagaaag ctggttcgat ccgataaagg aatttcggaa
    28921 atcttcctct gtagaatgcg catgatagac aacttttttt ccttctcttc tggctttatt
    28981 gaccatatta tgactattga ttccgtatgt attgatgtgc aggatgtcat aatcttcttt
    29041 ttcatctagt gtataagaga taccgacttc cgtcagagca cgtttttgat gatcaagcgc
    29101 acgcccgatt ccagattttg caagcatctt ttcactttca aaatataata ataccttcac
    29161 cttacgtccc ccttcactta tatagtataa catagcctac aaactgtcac tcaaacttaa
    29221 gccctatttt tgtctattct tactgacaaa aacgttttct taaaaaagaa caaaaaaact
    29281 ggcgtagctc ttttacaagc cacaccagct tttgatacgt tgtttatttt acatattcat
    29341 gaacaagttc aacgacttct tccatcgtat cgcattcttt caatgcacga tcagcaagtt
    29401 cagccatttt cgctgtgtcc aagcgtttca ttaaactacg cgttttaaga atagatgtcg
    29461 cactcattga gaactcatct agacccatac ctactagcaa tggaacagct gtttgatctc
    29521 cagccatttc tccacacata cctgcccatt ttccttcagc gtgtgctgca tcaattacat
    29581 ttttgatcaa acgtaaaatt gatgggttat atggttggta caagtaagaa acgcgttcgt
    29641 tcatacggtc tgctgccatt gtgtattgaa tcaaatcgtt cgtacctaca gagaagaaat
    29701 ctacttcttt tgcaaacttg tctgctaaga ctgctgctgc tggaatctcg atcatgatgc
    29761 cgacttggat cgtatcagat acttccttgc cttcacttat caatttttgt ttttcttctt
    29821 caaagatcgc ttttgctgcg cggaattctt tgagtgtagc taccataggg aacatgatac
    29881 gtaaattacc atgaacagat gcacgaagca atgcacgcat ttgtgtacgg aacatttcgt
    29941 cgccttgttc agataaactg atacgcaatg cacgatatcc taagaatgga ttcatttcat
    30001 gaggtaattg cagatatggt agttctttat ctccaccgat gtccattgta cgaacaacta
    30061 caggttttcc ttccatacct tctaatacag ctttgtaagc ttcatattgg tcatcttctg
    30121 ttgggaagtc tggagaatcc atatacagga attcagtacg gtacagacca actgcttctc
    30181 caccattatt atggacacca actagatctt ttggtgttcc gatgtttgca gctaattcaa
    30241 aatgcttgcc gtcagctgtg acagtttctg cattttttaa tttttcccat tctgcttttt
    30301 gtgctgcgta atctgcacct gctttttcaa attcagcttt ttgttcatct gtaggatcga
    30361 tgattacatc gccttcgatc ccgttgacag ccaaaatatc gccttctttg actttagcag
    30421 taatttcttt tgtcccaacg attgctggaa tttctaaaga acgtgccatg atagcagaat
    30481 gtgaagtacg tccaccaatg tctgttacaa acgcttttac aaaattgcga tctaattgtg
    30541 ctgtatcact tggagtcaag tcatgtgcaa caacgaccac ttcttcgttg atcattgatg
    30601 gatttggtaa agtaacgcct aataagtgcg ctaaaatacg tttagcaacg tcacgaatat
    30661 ctgcagcacg ctcttgcata taagcattat cttccattgc ttcgaacata ccgatataca
    30721 tatccgttac ttcttttagc gctgattcag catttacgct gttgtcttta atattttgct
    30781 tgatttgacc taccatttct ggatctgaaa gaaccatcaa atgggcatca aatacttgcg
    30841 cttctgcttc acctaagctt tgcgctgctt tttcacgaat ttgctgcaat tcttcagtag
    30901 atttagctaa tgcagcatcc aaacgagctt cttcagccgc agtatcttct actgttgcct
    30961 tactgaatgt taaatccggt tgaactagca ggtaagcttt tgcaacggct acaccgtcac
    31021 tagcggcaat cccttttagc atttcaacca ttattcagat aagccttctt tcttcattgt
    31081 ttcaacgatt gctgccattg catcagcttc gtcagcacct tctgcagtaa tagtaacatc
    31141 agaaccttga cctacaccta aagacataac gcccatgatt gatttcaagt ttactgattt
    31201 acctttatac tctaagttta catcagagtt aaatttgctt gctgtttgta ctaatagtgt
    31261 agctggacga gcgtggatcc cagtttctgc tactacgtga aattctttct tttccatatt
    31321 aatcggtctc ctttaagtga gtgttgtttt tatgtgaggg ttttcttttt tagtaattca
    31381 gagaatccta acaaatgcca ccctttcatc tgtaaagatt aacatttttt ttcgttagat
    31441 acaacagttt tcattcaatt tcagataaaa gtattcaaat tattccggat tctttttcat
    31501 ccttttcttc acattttata acaaacgtgt ttatttcctt tttcttgtcg ctttctcttc
    31561 tcctgacgtt tatcattctt tcctttgacc ctctttgttt tcatatgaaa cgaattactt
    31621 gcttcccttt ttatttttag tataataaga gtcaaaggtc aggaatggtc aatagagatt
    31681 tcaccttttc tacctcataa atctcttaaa aaactagggg ttattctttt acctcatgaa
    31741 aaacgagatg taaaatatgg atgtacccat ttttgtgaaa ggacgtgaat gattcatgct
    31801 ttgtcaaaac tgcggcaaga atgaggcaac gattcacttg tatgccactg tcaatggtca
    31861 gcggacacaa ctcgattact gtcagagctg ttatcaaaaa atcaaaaatc aacaaaatgg
    31921 aggtctgact aatatggccc aaaatgatcc atttggattt ggcagtttgg acgatctttt
    31981 ccgttccatg tctcgccaaa tgcaacagca aggaaattat gagcaaacac caccaacaca
    32041 atttggcgga aacaacaact tcaatggtgg tcagccacct caaggcaata cagaaggttt
    32101 attgggagaa tacggaatca atatcacaga acaagcacga caaggtgata tcgatcctgt
    32161 gatcggacgc gatgacgaaa tcaaacgagt cattgaaatt ttaaatcgcc gaaccaagaa
    32221 caatcctgtc ttgattggag aaccaggtgt tggtaaaaca gcagttgttg aaggtttagc
    32281 acaaaaaatc gtagatggtg atgtaccgca aaaattaatg gacaaagaag tcattcgttt
    32341 agatgtcgta tctttagtcc aaggtactgg tatacgagga caatttgaag aacgtatgca
    32401 aaaattgatt gaagagatcc gtcaagcaga aaatgtgatc ctattcatag atgaagttca
    32461 cgaaatagtc ggtgctggtt ctgctggtga tggaaatatg gatgctggta atattttaaa
    32521 accagcttta gctcgaggag aattgcaaat ggttggtgct actaccttaa atgagtaccg
    32581 catcatcgaa aaagacgctg ctttagaacg tcgtatgcaa cctgtacggg tagatgaacc
    32641 aactgtcgaa gaaacgatca gtattttgaa aggtttgcaa aaacgttatg aagactatca
    32701 tcatgtaaaa tatactgatg aggccatcga agcagccgca actttatcca atcgttacgt
    32761 ccaagatcgc ttcttgccgg ataaagccat cgatttgcta gatgaaacag gttctaagaa
    32821 aaacttgacg atccaaatcg ttgatcctaa aacaatcgag aaaaaactgc aagaagcaga
    32881 agaacaaaaa gttttagctt ctcgagaaga agatttcgaa aaagcagctt attatcgcga
    32941 tcagatcaat aaacttcaaa aaatgaaaga acgtcagctc actgaagagg aaacacctgt
    33001 gattaccgaa aaagatatgg aaaaaatcgt tgaacaacgg acaggtattc cagtgggtga
    33061 attaaaagaa aaagaacaga cacaactgaa aaacttagca gatgacttga aagctcatgt
    33121 catcggacaa gataacgctg tagatcgagt agcgaaagcg attcgccgaa accgtgttgg
    33181 attaaacaaa cagaatcgtc caatcggctc cttcttattt gttggaccaa ctggtgtagg
    33241 taaaacagag cttgcgaaac aacttgccta cgaactattc ggttcacaag attcaatgat
    33301 tcgtttcgat atgtctgaat atatggaaaa acatagtgtc tctaaattga ttggttctcc
    33361 tccaggttat gtcggatacg aagaagctgg tcaattaaca gaaaaagtac ggagaaatcc
    33421 ttacagtctt gtcttgttag atgaagtaga aaaagctcat ccagatgttt tgcatatgtt
    33481 cttgcaaatc ttagatgatg gtcgattgac agatgcacaa ggaagaacgg tcagctttaa
    33541 agatacgatc atcattatga caagtaatgc cggaactggt aaagtagaag caaacgttgg
    33601 ttttggtgcg gcacgcgaag gtgttactcg ttctgtattg aaccaattaa acaactactt
    33661 tacaccagaa tttctgaacc gttttgacgg gatcatcgaa ttcagtgcat tatcaaaaga
    33721 aaacttgatg acaatcgtca cactaatgct agacgatgtc aaccaaatgc tggcagcaca
    33781 gcaacttcac atcgaagttc ctacgaatgt caaagaaaaa ttagttgact taggctatga
    33841 tccatctatg ggtgctcgtc ctcttcgacg cacgatccaa gaacaaatcg aagatggtat
    33901 cgctgaattt tatcttgatc acccttctat ccatgagttg aaagcaaaac tggataaaga
    33961 tggaaaaatc atcgttactt ctaaaccaga acgtttggct aaagaatcag ccgaagaaac
    34021 ggctgaataa atccagtttt atagcgtgag acggtatttc tttgagaaat gccgtctcat
    34081 tcttttttca gaaaagtcaa aatactgttc attttataaa aaagaaatta taataagaga
    34141 aacaagcaat taaaaataca taaaagaggt gagcgccatg aaattggtaa atgtaacaaa
    34201 cagccattcc cgtcttgtat taaaccagct tgaaaacact gatgcccatc tagtcaaggt
    34261 ctacacagcc ggaaatacca ctattgtcta cactgaagcc cctcaacata atgaaattct
    34321 gctgatcaat gacaaacgta aaatccagcc gaaagaaatc gaagatgcta aaaattattt
    34381 cttagataaa atcaaagacg ccgtttacca cgaggatgaa atcaaagtaa tcgaattggc
    34441 aggccttgtt gaaatctcga ttccaaaaca tgaattaggc gctacattag ccgtgtaaac
    34501 aaattttgaa cataataatt aaagagcgtg ggacaaaagt aaag
//
LOCUS       DAZKNM010000012        32332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000012 DAZKNM010000000
VERSION     DAZKNM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.012"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..547
                     /gene="nrdI"
                     /locus_tag="ACG8M9_000488"
     CDS             101..547
                     /gene="nrdI"
                     /locus_tag="ACG8M9_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0377287.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(612..2594)
                     /locus_tag="ACG8M9_000489"
     CDS             complement(612..2594)
                     /locus_tag="ACG8M9_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0377288.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2608..3504)
                     /gene="murQ"
                     /locus_tag="ACG8M9_000490"
     CDS             complement(2608..3504)
                     /gene="murQ"
                     /locus_tag="ACG8M9_000490"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0377289.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3507..4568)
                     /locus_tag="ACG8M9_000491"
     CDS             complement(3507..4568)
                     /locus_tag="ACG8M9_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0377290.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4779..5306)
                     /locus_tag="ACG8M9_000492"
     CDS             complement(4779..5306)
                     /locus_tag="ACG8M9_000492"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0377291.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(5373..6875)
                     /gene="eat(A)"
                     /locus_tag="ACG8M9_000493"
     CDS             complement(5373..6875)
                     /gene="eat(A)"
                     /locus_tag="ACG8M9_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0377292.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7133..8254)
                     /locus_tag="ACG8M9_000494"
     CDS             complement(7133..8254)
                     /locus_tag="ACG8M9_000494"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0377293.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8380..9321
                     /locus_tag="ACG8M9_000495"
     CDS             8380..9321
                     /locus_tag="ACG8M9_000495"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0377294.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9471..10367)
                     /locus_tag="ACG8M9_000496"
     CDS             complement(9471..10367)
                     /locus_tag="ACG8M9_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0377295.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10636..11976
                     /locus_tag="ACG8M9_000497"
     CDS             10636..11976
                     /locus_tag="ACG8M9_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0377296.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12194..12637
                     /locus_tag="ACG8M9_000498"
     CDS             12194..12637
                     /locus_tag="ACG8M9_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0377297.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13141..14559)
                     /gene="nhaC"
                     /locus_tag="ACG8M9_000499"
     CDS             complement(13141..14559)
                     /gene="nhaC"
                     /locus_tag="ACG8M9_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0377298.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(14962..16185)
                     /locus_tag="ACG8M9_000500"
     CDS             complement(14962..16185)
                     /locus_tag="ACG8M9_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0377299.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16216..17055)
                     /locus_tag="ACG8M9_000501"
     CDS             complement(16216..17055)
                     /locus_tag="ACG8M9_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0377300.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17072..17752)
                     /locus_tag="ACG8M9_000502"
     CDS             complement(17072..17752)
                     /locus_tag="ACG8M9_000502"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0377301.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17754..18827)
                     /locus_tag="ACG8M9_000503"
     CDS             complement(17754..18827)
                     /locus_tag="ACG8M9_000503"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377302.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(18883..19121)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19164..19397)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19474..20145)
                     /locus_tag="ACG8M9_000504"
     CDS             complement(19474..20145)
                     /locus_tag="ACG8M9_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0377303.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20232..22880)
                     /gene="mgtA"
                     /locus_tag="ACG8M9_000505"
     CDS             complement(20232..22880)
                     /gene="mgtA"
                     /locus_tag="ACG8M9_000505"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0377304.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(23222..23396)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            23569..24006
                     /gene="ndk"
                     /locus_tag="ACG8M9_000506"
     CDS             23569..24006
                     /gene="ndk"
                     /locus_tag="ACG8M9_000506"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0377305.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(24028..24894)
                     /locus_tag="ACG8M9_000507"
     CDS             complement(24028..24894)
                     /locus_tag="ACG8M9_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0377306.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25054..27147)
                     /locus_tag="ACG8M9_000508"
     CDS             complement(25054..27147)
                     /locus_tag="ACG8M9_000508"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0377307.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(27896..28519)
                     /locus_tag="ACG8M9_000509"
     CDS             complement(27896..28519)
                     /locus_tag="ACG8M9_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377308.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(28678..30075)
                     /locus_tag="ACG8M9_000510"
     CDS             complement(28678..30075)
                     /locus_tag="ACG8M9_000510"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0377309.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(30107..31078)
                     /locus_tag="ACG8M9_000511"
     CDS             complement(30107..31078)
                     /locus_tag="ACG8M9_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0377310.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            31452..31877
                     /locus_tag="ACG8M9_000512"
     CDS             31452..31877
                     /locus_tag="ACG8M9_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377311.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         9247 a         6778 c         5636 g        10671 t
ORIGIN      
        1 tctcattata caagcaaagc gcctagtccc tttccaaatt acaccaccaa aaaacttaaa
       61 aaagatgcta tactaaaagg aaagaattgg aggatgatcc atgaacattt tatatatatc
      121 gatttcagga aatacgcgtg cctttgccaa acatctagca gaatatgctg aaaaaatgca
      181 tgcagaagat cctgtcaatc ctgaagttac tttaaaagaa atccatgaaa atagtgattt
      241 tacgaaagaa acagaaccat tttttacttt cgttcctact tatttagacg gcggaaatgg
      301 tttagataac ggtgatacag aaatcttgac cgaaacgatg cgtgaatatc tggaacatga
      361 agacaatcat aaactttgtc ttggtgtagt cggtagcgga aataaaaact tcaataatca
      421 gtattgtcta acagccaaac aatatgcaca aacatttggc tttcctttct tggcagatta
      481 tgaattacgg ggaacaccat ctgatgtgac acgaatttat cagattttag cagaaaacaa
      541 taaataataa aaaagaagaa gctgaaaaat agatttcagt ttcttctttc gtttctattg
      601 tttctatcat attaagcttt gacatccatc acaggtgctt ttgcttttgc tttacctgtt
      661 ttttccaaga caaagttttt tacacgatcc atattggtta ccaagacgat catggatgtt
      721 tctttgcctg ctgctttgat cgcttctaag tcagcttctg ctacaagcgt atcaggtgtc
      781 acttgctgcc cttctgttac tttcacatca aatggcgtac cgcctaagtc aactgtgttg
      841 attcccatat gaagcaagat ttctaaacca tttggcgttg tgatcccaat agcgtgtttc
      901 gttgggaaga cagagctgac taccccagta actggagaat agatatgtcc gttttctggt
      961 tctacaaagt agccttcacc catcatcttt tgtgcaaaaa ctggatcact tgctttatct
     1021 aaagaaacaa ctgttccgtc agcaactgat gagaggatag tcacactttc agctgaagtt
     1081 tgaggtacta ctttttcagg cacttcattt actgtggctg tttcagtcgc taattcttct
     1141 ttttctaaac ggtctttagg aataccgaag aagaatgtag caacgaagcc accagcataa
     1201 gctgccaata atccaaggat gtagcctaac catttaccgt cagagatcaa tgggatcaaa
     1261 gcggctcctg aaggtccaat agcgatagcc ccgacatttc cgatcattcc gatcactgcc
     1321 ccgccgatac ctccaccgat acaagcagta ataaatgggc gtcctagagg taatgttact
     1381 ccgtaaatca atggttcgcc gattcctagg atcccgactg gtaaagctcc tttgatcatt
     1441 tctgttaatt ctttatcttt tttgcagcga atccataatg ccaatgctgc acccacttgt
     1501 cctgcaccag ccatcgcaag aattggaagc aatagcgtac gaccagtttc atcaatcatt
     1561 tgcacatgga ttggagtcaa aatttgatgc agtccgaaca taaccatcgg caagaataat
     1621 gtgcctagaa cgaagccggc aaatgctcca ccaacgccaa gtacccagtt gatcccaccg
     1681 accatgccat cagaaatgaa acctgctaaa ggcatgatga agaaaatttc aatcagaccg
     1741 atggcaatca atgaaagagt tggtgtaacg atgatatcta ccgcatcagg tactacttta
     1801 tgcagttttt tctcaacgat tgacaatagc caaacagcga agatgacacc taggatccca
     1861 ccttgtccag ctgcaagagc ttcaccagta aataagtttt tcagtggtgc ttccggattc
     1921 atccctgtca gcatcacaac ggccccgata actccaccaa gagttggtgt tgcaccaaaa
     1981 acttgtgcag cattgatacc tgtatagata actaggtagc taaacatccc gtttttcaag
     2041 atgttcatca catcgatata ttgttgccat gttgctgcat caagtgttcc ggctgtgact
     2101 aagtttgcta aaactgcggc aatccctgcc acgatcccag taccgacaaa tgcaggaatc
     2161 attggcacaa agatattaga aatgtctttt aataccgatt tgattttaga tggtttttgt
     2221 ttcgattttt gtgccgcttt catttcttgt gcccgttcgt ttacacggtc ttttccagat
     2281 gctgatgaat ttgctgtagc tgtacttaca gaagaaagtt tttctcctaa tttcacgcct
     2341 gccaaatcaa ccatttgctg tgctactttg ttcactttcc caggtccgat gatgacttga
     2401 agctgttcat cttctactac accaagcact cctggaatcg cttttaattc agagacattt
     2461 acaagatcat cattgatgat cttcatacgc acacgagtca tgcagtggac aatgctttct
     2521 acattgccca tgccgcctgc ccctttataa atttgttttg ccagttcttc caaagtcagt
     2581 tcgttttttg ccatggtctt ttccccctca taaggtccct tttacaaatc cgtttgcttc
     2641 agctaatttt gcttgagctt cctctttatc actatttgtc aaaatcatga cgatagccag
     2701 cttaacattt tgatctgctt cttcaaagta cttttcagct gtttcgtaat cagcttctgt
     2761 cgcttgcatg atgattcgtt ttgagcgttc gattagtttt tcatttgttg gtttgacatc
     2821 gaccatcaag ttattgaata ctttacctat accaatcata ctgatagtag aaatcatatt
     2881 caagataagt ttttgtgctg ttcctgactt caatcgagta gagcctgtca aaaattctgg
     2941 acctgcatcg acttcgattg ggaaatccgc gtgtttactg atttcggctt gtttattgca
     3001 agaaatcgtt cctgtgacag caccgatcga ttttgcgtaa tctaatccac cgatcacata
     3061 tggtgtacga ccgctagctg cgataccaat aactatatca ttttttgtca aattcagctt
     3121 gattagatct tcttgcccta gctcataaga atcttcggcc ccttcgacag ctacagtcat
     3181 tgcttcttct ccaccagcaa ttagacctat gaccatttcc ggttctacac cgaatgtcgg
     3241 tacgcattca gcagcatcta acacacctag tcttccactt gttccggctc ccatataaat
     3301 cagacgtcca ccttttttaa aggactcaat cgtgttagca atcactggtt cgatacttgt
     3361 cagttctttt tcaaccgcag cggccacttt ctgatcttct ttgttcatta gttgaacggc
     3421 ttgagaaata ctcatctcat ctaatccaaa agtttcttgg tttctgcgtt ccgttgttag
     3481 gttctctaaa ttgattttgc tcatttttat cttccttctt ttcttataaa ttgatagttc
     3541 ataccagctt tgatttggtt gatcaatggt agctcttctt tgataacttt agctactaca
     3601 ttcacttttt catccgctgg caaatcatat ttggttaatt ggatctcacc catatagcgc
     3661 aaatactttt cgttatctaa agtaatagac cctttcatac gcgtagctgt atttcttgct
     3721 gggataggcg gaatctctcg aaaacgtgca tctacgcttc gtatgacatc tcttgcatcg
     3781 tcttgccgat tcgtatgctt ccctaatata tattcgtaaa attcttcatc tacaggttcc
     3841 gtatggagca agactgtctg ttctttttgg aaagaagcaa attgctcctg aacgttttct
     3901 gacagacctg catctccgat gtaaacaata tccgtgttac ttgctaatag atctagagcg
     3961 cctgcaagag gatggatccc tcgatgttct tccaaagttg gcaatccttg atatagtggg
     4021 cctctcaact ctgtatctcc tggtacaaaa ccttggacaa ggagaccttg aattttgagc
     4081 cattgatttt ttttctgata ccattctttg tccagccctg tttcgggacg tggataataa
     4141 ttatgccaag cttcaatctg tgagaaatca gcttctgctt ctttcaattc atcgatatct
     4201 ttaggcgtga tcgtgctagc attcaaactg attttcaatt gatgtgacca gtcggcaatc
     4261 tgctgattgg aaatatgata atccattctt aaacctgtta caccaattgc tttcaaaggc
     4321 gttagatctt ctaatgaaaa acccgctcgt tccaatgcct ctcctgaaat atcgaccatc
     4381 aattctagtt tattcttttt cgcttcgctt cctagttcga tcaatcgctt ttgatatgcc
     4441 gaaacatcat cttctggtat atgcatggaa gtgaaaatac cgacaaagcc attggatgcc
     4501 atttggcgga tatactgttt cttctcttct gacaaagaat cgttcataaa tattgaaaag
     4561 ccaaacatat tatccctctt tttctgtatc cgtcttcatt tctatgacta gagtatatat
     4621 ctaaagaaac aaaatttcaa tacttttatt aaaataatta aatttcattt ctaaaggttg
     4681 agtttatatt atcaaaagaa ttgtcataac ctaaaaaagg cactgctaag caaaagcagt
     4741 gccttgaaat attttattta aataacacaa aaacataatt actcataaga aaccaattct
     4801 gtattatcta ccatgaatgt aaagaacccc tcattaggca tcggatgaaa ataagcattg
     4861 atcactctta tcaaggcatt atggcaaact aacagataga cattttcttc gtcttcgatg
     4921 cattctttca ataaaggaac gatccgtgca tacacatcta agactgactc cccatttggg
     4981 aatcgtacag cgaacgctaa acgtgctttt tgaaagttct catctttgct tggcagtcca
     5041 tcataatccc caaaatccat ttctaccaat cgttcatcaa cggtcagcgg caagctgaga
     5101 cgatcagcaa ctgcttgtgc tgtatctctt gctcgttgaa gaggggaatg gatgattttc
     5161 gtgattggaa caggcaaatc tactaccttt tctgctagtt tctgtgcctg catctgtcct
     5221 ttttcagtca gcggcacatc tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt
     5281 tctccgtgtc tcgttacata aagcatgtga ttctctcctt cgttggcaaa gttcccctta
     5341 accatacctt gttgttcttg agattacaag ggttagattc tctcaagaga aataatctca
     5401 gtagatactt tatccaggaa ggtttgatca tgttccacta gtaacatggc aggtttcacg
     5461 ttcaagatca gttgttctaa ttgttcttga ttgaagacat ccaaataatt caatggttca
     5521 tcccatgtat atagttcagc tggctgtgat aaagatttag ccaattccac ttttttccgt
     5581 tggcccatac tcatctgctc gatcttgtta tgaaaaacat ctctttccat cccaagcttt
     5641 cggaggttgt tcaaaaatgc ttggtagtct acttggtttt tctctgcaaa ttccgccaac
     5701 gttcctcgat tgtcttcata attttggctt gcataactaa tgctcagatg ttttggctgg
     5761 tatttttctc ctataacctt gccgttgaat gcccccagaa gataatggat aatggatgac
     5821 tttcctgcac cgttaggacc tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga
     5881 attggctcga ataacagatt ttcataccct aattgaagat cttctacgct taaaagtcgc
     5941 ttatggtgag acgcttggct attcatcgtc aaagaatcga tatattcgat atcttttaat
     6001 agtttttctt tctcgctgat ctgggtctcc atccgattca cgattgcttt ggatcgtttc
     6061 atcgtccgtg cagcatcagc acccactgct cctttattca ctcgtctaga ttcagtatcg
     6121 atgaatccga cttgtttctt tgttttatct ccttctcggg aacgagacca ttcggctttt
     6181 tcagctgctg ttttctttag cctactgact tcttttttca atttttcatt ttgagccatt
     6241 tcaaattcat cacgaagttt tttctgttct tcatagatag agaaattccc ttgataaagt
     6301 tccagttgac ttttttcgat tgctaaaaca tggtccacta cttcatcgat aaatccccgg
     6361 tcatggctga ccacgatgaa gccttgtttt ttctttttca aataagccgc tacttgtttt
     6421 ctaccagaga tatccaaatg attcgttggt tcatcgatta acgggaaatg agtgtcatcc
     6481 acaaataaaa gtgccagtag gacttttgtc ttctctcccc ccgatagtgt gctgaattct
     6541 ctccataaga tttcaggatc tgtttgcatc aattggagct ctctttcgat ttcccatatc
     6601 tcaaaatccg taatatcatt taacacgtaa taggttaaac gttctttatc ttttgtctgt
     6661 tgcgggaaat aggcaaattc ttgctgatgg attacttgcc cttgataagg tagtttgttt
     6721 tgtagaatat tcagtaaagt tgtctttcct cgaccgtttc gtccgataag tcctagtttc
     6781 cattgtgtgt caaaatttag atttgcttgt tcaaataata atgtgccttg gctgtcgtag
     6841 ccgaatgtca gattttttat ttcgatttta gacattttcc atcacctcgt aaaaaaagac
     6901 cagtcctatt ttcagaactg gctaaagagg atgcaagggt tggatacaca caaaaagacc
     6961 catacatcga aaaaaccaag ccaatcccga aaatctgcac actgcttcct tgtctccaca
     7021 aggtacagtc tgaatgcagc tttcatcaat cgacttaatt tttcaatgca cggatccctc
     7081 actttgtcct atatttgaaa ttacgatagc ataaagaaaa tgattttgtc aactacttcg
     7141 ggaggatggt gaaaaatctg ctaagtgtct gtcctaagat ttttgttttt tctaatgttt
     7201 gcctgtgttc cattgcttct tctaaaccta ttggacccaa atgaatactt aagacaattg
     7261 ggagaaaatc agaaagtcct tctagctctt ggtctctact tccgcaaata gcgattattg
     7321 gttttcgata tttttttgca agcactgcta caccgtatgg aacttttccg tggatcgttt
     7381 gctgatcgat ccgcccttct cctgtaatga tgacatctgc ttgttcgatc tttttttcca
     7441 atccagtgat ttggctgact aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa
     7501 gggctccacc aataccgcct gctgctccac ttcctggaat atcatctaaa tccacctccc
     7561 agaaatcttt tatttgctga gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg
     7621 tatctattcc tcctttttgt cttccaaatt gccaagcagc tccttgttga cctgtatatg
     7681 gattggtaac atctgcagcg ataatcaatt ttactttaga aacaagaacg tttcttcgat
     7741 caaaatgaag ttttttagta gataataaag gatttccttc tgtgtaccct tctaaagaag
     7801 cacccaagga ttggagtaat cctagaccac catcgctaca cccgcttcct cctaaagtga
     7861 caataatttg ttctacttct tgaagcagtg catctttaat aagttctcct aaggcataac
     7921 tggaagcaag tcggatagtt tgatccgttg gtttatcaag tagatctaac cccaatgctt
     7981 gtgcagattc gatgactgcg gtgttttttc cttgccaagt tgtcaataaa tagctgattt
     8041 ttcgctgacg gaataagaga tccatcccat caatggtacg ccatgttccc ctgagcgcgt
     8101 aatagattgc ttctgcagta ttttctccgc catctgcaat cggcacattg atttttcttc
     8161 ctttacgatc ttcccagcca tttagaactg cctgatttag ttcaaatgaa gaagcacttc
     8221 ctttaaatga atcaattgcc gccaaaatat ccatctgtat ccctcacttt cttcttaagt
     8281 aagacacttt ttttcatttt tttcaaacaa aacaatgcta ttttcttcca tttttctata
     8341 tactaaaagg gaactcattg aataaaggag agaaaaaaca tgaaaatcgc aatagctggg
     8401 gccggagcaa tgggcagccg aatcggattg atgctacatc aaagcggaaa tgaggtcctc
     8461 ttgatcgatc gttggcctgc tcacattgaa gccattcgta caaatgggtt gattgctgac
     8521 tttaacggta aagaagtggt cgcaaaactg ccaatctatt caccagaaga aatcatcgaa
     8581 agcaacgaac acgtggactt gatcgtagca ttaacaaaag ccaatcaatt agatgatatg
     8641 ttttgttcga tccaatccat tattactgac aatacttatg ttttgtgttt actaaatgga
     8701 ttaggtcatg aagatgtact ggaaaaatat gtgccaaaga aaaatatctt atttgggatc
     8761 acgatgtgga cagctggtct agctggtccc ggaaaagtca cattgttagg tgatggagaa
     8821 atcgaactag agaacttgga accagaaggc gaagcattta caaaaaaagt tgtagaagtc
     8881 tttcaagaag caaatttaaa tccgatctat agccataacg ttcgttattc catttggcga
     8941 aaagcttgtg taaatggaac attgaatggt ttatgcacga ttttggattg taatattgct
     9001 gaactaggcg cgcaaaaagc ggcagagagc atggttcgaa caatcgtatc tgaatttgct
     9061 tctatcgcag caaaagaagg aatcatacta gatcaagaag aagtgtacca acatattgct
     9121 tctacttatg atcctgacaa catcgggttg cattacccat ctatgtacca agacctgatc
     9181 aaaaaccatc gtttgactga aatcgactac atcaatggtg ccatttggcg aaaaggacaa
     9241 aaatatgata ttgctacacc atattgtgcc tttttgaccc aattagtcca tgcaaaagaa
     9301 ggaatccttg gcgcagaata aaaggctctc tgatgagaat cagctgaatc agcagctggt
     9361 tctctttttt taagcccaat aaaagatcaa aaagaaaaca caactctcat gatgccataa
     9421 gaattgtgct tgtttgtttc agtacataat ctcttcaaaa tgtaggtttt ttatttgttt
     9481 tcaacagagg agaggtcata ttcgtattgc tcataaatag tggaatcgtt agtgataaat
     9541 cgttttgccc aattttctgc atcataaaat tccaatagct ctgataggat tggcttaaat
     9601 ggtgtatgca gaaattccgg taattcatct aaatcacgtt ctcgaagcaa aaaaatattt
     9661 tcaggacgat aaaaatgata atgagcaatc gcttgattat ccgtcactag tttcttttga
     9721 aaatacatgg attcaaatgt ccgcaaagtc actccttgct gaccctcccg caacagttcc
     9781 agaatcccgc gcgattcccc tgtttttttc aggtactctt cataagtaag atatccaggt
     9841 tgttcattcc ctcgatgtgg aagaatgaaa tagttggtcg taacatctct ttttataaat
     9901 tcctgcttat agctttctgc tcgttctttt ctcccattat tcgttgcacc aaaaaataga
     9961 tccgtctttg gttcgctaga agaaaaattc atatgtttcg aataaaatga actattatgt
    10021 ttcagcccaa gttcttttgc ttccaaaaaa tcaaaatgat aaatctcatc gattgccggt
    10081 tcatcttgtg cctgcttcag catcgcctcg tgttctgctt tgaagtgatt ccaaaaaaat
    10141 aacacaattt ttgcattggt aaatgggcgc acgcgtcgag ccatctccat atcaaatcca
    10201 taatctaaaa aaacgatcag ctgataattt gccaactgtt gtctagctga tttaatgaat
    10261 tccgactgta gtaaatcttt tccggaccaa acatctaaag agcggatgga aggcaaaaaa
    10321 taaagtgatt tattttttgc aactagcagt acgtcatttt tgttcatctt ttccgcctca
    10381 ctttctctgt cactttttcc tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgt
    10441 ttcctatatt tgtatgaaaa tttaacattt gtatgaaaat atgatgaaat aaatcattct
    10501 tctattaaaa ttcgcctaaa agttttctga atagttgtaa ttgtctgaaa atttgttatc
    10561 attagtcatt gttaaatatt gctatttatt cttaatataa attttgattg tgaatttaaa
    10621 agaaagaggt catttatgga agataaaaaa ttgaaaaaac aacttagctc cagacatatc
    10681 accatgttag ctttaggtgg agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg
    10741 attgggatcg ccggcccttc tgtattgatt gcttttttga ttggtgggct gattttatat
    10801 atcgtgatga aaggtctggg aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta
    10861 tctggtttga tacgccctta tctaggtgct cattctgctg attttactga ttgggtctac
    10921 tggtctatgt ggattatcaa tatcatcgct gaagctgttg cagctgcttc ctttttgcag
    10981 ttgtggttcc cgcacattcc agcctgggta tttgttttta tgttggctgt tttaactacg
    11041 atcatcaatc tttattcggt acggttattc gcagaaacag aatattggct ggcttttgct
    11101 aaaatcagtg tcattatctt actgatcatt tttgctactt atcttgtcgg tcagcaaatg
    11161 ttgggcacag gcgttttccc tacccttcag caattgactg accatggtgg gtttactcct
    11221 catggtatga agggaatcat cagttctttg cttgtagtta tctattctta cggcggttct
    11281 gaactgattg ctatcacggt aagcgaagca gatgatccta aacgtgcgat tcctaaagca
    11341 atcaaaggtg tcatgggaag aatcatttcc ttttacatca ttcctttgtt tttgcttttg
    11401 attctttttc cttggaatac tttagcaggc acgaatgtca gtccttttgt gatggtattt
    11461 gaaaaaatga atatcccatt tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg
    11521 ttctcatcta tcaattcggg tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag
    11581 gatcgaaaag gttcctctag aaaactagct gttttaaaca agcatcaagt accgcaacgt
    11641 gctgttttat tttgtaccag tgttctttat cttggtgtag ctctttctta ttttgtcggt
    11701 gacaaattat ttggctatct ggcaggttcc ctttcgtata cagtattatt gatctggatc
    11761 attatcagtg cggcatcctt tgtactggca ctaagaaaag gaacattatg ggataaagga
    11821 atcagctttt ttgcattagc tgtcttagga ttgattttcc ttggtatcct gttcacgaac
    11881 tccattgggg taactgtatt gactggcctg ctttacctgt tcatttattt cagttatcaa
    11941 aaaaagaatg atgcgttcgt attgacgaat gaatgaaatc gatagaaaat tttattttat
    12001 ttatgtggaa tagatggagc tattgaagaa aggagttgtg acaaaagtct tgttgcaact
    12061 cctttttccg aataaacagt ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt
    12121 gcaaaataat tgattgttct ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag
    12181 gagtttgttt taaatgaaaa agtattggaa gtttttgttg atgatcggtg tttcaacggt
    12241 agttatgttt ggtatgatgt acttgaatgt ctatgcgctc gatcatatct tcttcagtcg
    12301 tacacgagta ttcatggcgt tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt
    12361 catgtggaaa atgtatgaaa acaaaaagat aaatgttatg atcttagccg ctagtcttct
    12421 tgtttttgcc ggttccttgt ttatggtacg aagccaaact actgtcaacg acacagcttg
    12481 gatgaaggcc atgattcctc atcattctat cgctatcctg gcaagtaaac gagctgattt
    12541 aaaagatcct gaagtaaaaa aattagcgga agaaatcatt caggcacaag aagaagagat
    12601 cgacaaaatg aaaaaaatga tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa
    12661 agaggatgtt ccagaagtta tcagcttcaa gaaataagaa gaagctactt ctggaacgcc
    12721 gtgaataagg ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    12781 atagcttctc ctatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca
    12841 ccgtttatta agaggttgta aaatcagcgt tctgacctga gtattagaga gacaaatgga
    12901 aaaatagttc ctcatatttt gccatttttt gagctaatac cgagggtcag ctgattgaac
    12961 accgtttatc gagaggttgt gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt
    13021 gaaatggctt tccacatttt tgtataaatt tggcttaata ccgagaaact ggcttttgaa
    13081 taccgtttat tcgtaaaagg gattgtgaca aaagtcttgt cacaatccct ttttatgttt
    13141 ttattgttct aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa atttttttga
    13201 gattccccaa cctgtgaagg cactgaccat cgaaaacaca ggagaaagca aactgaaaaa
    13261 tacgaatggc aagtaagtga gtgtaggaat accaaatgta cttgctacga aacttccggc
    13321 aattccccaa ggcactaagt agttgattac tgttccacca tcttctagcg ttctacctaa
    13381 tacagttgga tcaaatcctg ctttttcata agtttcttta aatgcattac caggcagaat
    13441 aacagatagg aattgttcac caataaaaat atttactccg atacttgtca tcaatgttgt
    13501 aaaaatcaaa tttggtgtgt tcgtcatctt tcttgatact cgttccatca cggttgagat
    13561 caagccaaat tcgatcaaca atccacctaa tgacaaagtt agaataatca atgacacagt
    13621 aggcatcata ctttcgattc ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt
    13681 atttgaaaca aaccctgatt cgatgacccc agccaacttc gttaaggaag tttgtgggtt
    13741 ttgaataaaa atcattccta ctgctacagc gatattcaac aaaatcgtta aaattgccgg
    13801 cacctttttc catgcacaca gtaacattaa aaataaagga atcagtgacc agattgaaat
    13861 agtaaagttt gcttctagaa catcagtcac ttcacgaata gatgaaagac ttgcggcttt
    13921 acttgtgtgt cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg ggacagttga
    13981 ccacatcata tgcttgatgt gtttgaacaa atcggcatca acaatagctg ctgctagatt
    14041 tgttgattcg gataatggag acatcttatc tccaaaaacg gcacctgaaa caatagctcc
    14101 aacaaccagc gctggattta agcctagtgt tgtcccaata ccgaaaaatg caatcccaat
    14161 cgttgacata acagtaaagg cactccctac agcgctacct acgattgcac acacaacaaa
    14221 tacagaaggg acaaaccact ggacactgat caatttgaac ccaatgacca ttagagttgg
    14281 aatgatccct gcttgtatcc atacagcaat caatgaaccg actaaaataa aaataaagat
    14341 tgggatgatt cctggtttga tgccattgat gattccttcg tgcagccaat ctgtcgggta
    14401 tctttttatc actgcataca acatgaccat tccgatcgct gctaagatcg tgatattagg
    14461 tgagaccttt aatccgacaa cgccaatcgc aatgcctaaa acaataacca ttaatacaag
    14521 tgttgcttct aaaaaacttg gtgttccttt ttttcccatg ataatctctc cttttttagt
    14581 tgttcttatg ttctaaaaaa gttaagatat cggattactc cgccgcaatt catccgcgat
    14641 tcctccttta taaaaataaa aagacctgtt ttaaactaag gacgaatgac tccgcggtac
    14701 caccttaatt aaaaacagat ctgtttttca ctttcaaact tttattcaaa tgtatggact
    14761 ttccatgtaa ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac tttctgaaca
    14821 aaacacctaa ccgctactgc gtgaaaagtt gacttgtacg aagtttacct tccataagat
    14881 catttgtcaa tgaaaaagaa aatatttttt catttttctg aaaattattt ttataacttt
    14941 cacacacaag attatttttt actagaaagt agcgccctct tgtttttttc ttgctactat
    15001 ttcttgatgt tcttgcttga ttctttctaa tagctttggc tctttcaaca gatcaagagc
    15061 tgtattggcg agtagttctg ctgcaatcgc aatagaagca aggcctttgt cacttttagc
    15121 agcagctttg aattcttctg tgtgtccggc aatatatgtc tcagagattg ctaccgtagg
    15181 ctggattgtt ggtatgactt ggctgacatt tcctacatca gaagaaccga gacttgttct
    15241 ttgttccgtt tctatttctt catctggaac acctgcttct tttgcatgct ggaagaataa
    15301 gtcatcaaaa gaaggagtaa cgatgacatc atcaactgca ttttggaaca atccaaactc
    15361 aaaagtcgtg cccgtactta tcgcagcacc tcgtacgata ttttccactt tttcatatac
    15421 tttatctaat gtttgccgat ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg
    15481 aatgacgttg gcagctactc ctccgtctgt aatgatccca tggatattga catcttttgg
    15541 caattgcaag cgtagagcgt tgattccatt aaacacttgg atcaatgctt ccaaagcatt
    15601 tactcctttt tcaggtgctg ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac
    15661 tggatcattc gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacgcataa
    15721 cgctacatcg acatcctcaa aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt
    15781 ctcgccccct tcttcccctg gggtgccata tacacggatt tctccaccag tttcatcaat
    15841 gaattgcttc actatgctgg cggctaatac ggagtaagta ccaaacaaat tatgcccgca
    15901 tgcatgacca atacctggta aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg
    15961 tttattggaa atataacgtg catcaaaggc tgttcggtgt ccagctactt ctttttctac
    16021 ccaaaacctt tctttttcta attgtttaat caacgctgct gaggaataaa actcatagtt
    16081 acttacttca ggatgttcgt ggatatctag agcaagtgct tgatattctg gtaaacgata
    16141 ttggattaga tccttcaatt ggtttgttga gattgttgtt gtcataaaaa attcctccta
    16201 tgctagtcat ttttcttatt ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt
    16261 tgatgttttc tcgattactt tgactgtgtc ttctgtttga taagcgtcta ctattttttg
    16321 atacgtttta ttgttttcat cttcttttct tgcaacgatg atattcacat aaggacgtgc
    16381 atggtcatcc actggttcta agaagatggc atcagaagca ggattaaatc cagcatctac
    16441 agctacaccg ctattgatca ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga
    16501 agcatctaat tcagaaatgt ctagattttt tctattctcg gtaatatcag aaacagtggg
    16561 cgcttgtcct tttgccttat ccactttgat caatccagca gtttgcaata acagaagagc
    16621 tctgccgcca tttgtaacat cattagggat tgctactttt gctccgtctt tgatatcagc
    16681 tacatccttc aaagtgttag aataaattcc cagcggtgca ttgacagtat ttccgatcga
    16741 tactaaatca gttccgtgtt ctttattgta attgtctaaa aagaattgat gttggaaaga
    16801 attcaaatca atcgatccat ctgctaaagc cacatttggt tgattgtaat ctgtaaattt
    16861 cacgtattcc aaattaatgt tctccttttt caaacggtct ttcacgttgt cccatacatc
    16921 tgtatcttct ccaataatcc ctagttttac gatagtttct tttttgtcag ttgtatctgc
    16981 ttttccttgg ctacaccctg ccaaccaaat aattgctaca aatgtaatga ctacaaaacg
    17041 aataattttt ttcatttttt ctcctccact ttcaatgtgt aattttttta atcagttgat
    17101 tactgataaa ttgacttaaa aaaactaaga ttagaatcaa taatgtagcc acaatcgtta
    17161 catctgtttg gaatcgatta taccctctcg atatagcaag attccctaat ccgcctgcac
    17221 ctactgctcc agccatggta gtcagtccga tcacgttgat gatagtcaac gccgagaccc
    17281 gaatgatacc aggcaaacct tctaacaaat acacacgtaa aatgataccg attggactgg
    17341 ttcccatcgc ttctgccgct tcgatcaccc caggatcaac ttctagcagt gcgttctcga
    17401 tttgacgagc aaaaaacgga acaactccaa aaatcaatgg cactaaagct gctttcgcac
    17461 caatcgtcgt tccaactaag aaacgagtag tcaatgctaa aagagaaagc aagataatga
    17521 aagggatgga acgaatgata ttgatcagct tatcaagtat ttcaaatagc agacggttag
    17581 ctagtaatcc attagggcgt gtcactacta acgtcacacc taagatgatt ccaatcatcc
    17641 cgccaatgaa tgctgtccaa aaagacatat atagtgtttg cttcgtcgct tcgacaaatt
    17701 catccggaat caaataaaca ttgggcaaat atgttttaaa gaaatcagtc attttatctt
    17761 cctccttcga tcactttgat acgactatct aaatcgtttt tttgtaaaat atttattttt
    17821 acaccagatt tgatcaaata atccaatgct tttttccgct gtttggtttc tccagctaaa
    17881 gtaacgatca aactccctaa cggcgtttct tgtaataact ctacattgcc atataaaata
    17941 ttcgctgtta cttgatattt gctgtacaga tgagcgatta gaggttcgtt cgtctgattt
    18001 ccaatataac ttaattccac cagccattta ttggcgatct gatcggcaaa agcagaatgg
    18061 gcaatgatcg tttctaatgc ttggtcaaga tgagtagctg ttcgaataaa atctttggtc
    18121 aattcttctt caggattgct gaagatctgg atgctgctac ctttttcaac gattcttcca
    18181 tcttccataa ccgctacctt gttgcagatt tctttgacaa cttgcatctc atgagtgatc
    18241 aggacgattg ttaaacctaa ctggcgattc aattttttta acaatgcaag gatctgaagg
    18301 gtcgttttcg gatcaagtgc actggttgct tcatcacata ataatacctt aggttcattc
    18361 gccaaagctc gtgcgattgc tactcgttgt ttttgtcccc ccgacagttg tttaggataa
    18421 gcagatgctt tttcttccaa accgacaagt tccagtagtt cattcacttt ttgacgacgt
    18481 tcctgtttcg attttccaga atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg
    18541 ctttccatta gattgaaatg ctgaaagatc ataccgatgg cctttctttc ttttcgcaat
    18601 tctttgggag agagctgact cagattcgtc tgattgacaa tgacttctcc tgctgttggt
    18661 ttctgcagca aattcatcac acgaactaac gtacttttcc ctgcacctga gtaaccgacg
    18721 attccataaa catcgccttt ttcaatcgtt aaatctacag aatctactgc ctgtatctct
    18781 ttttcttttt gtttgaaaca tacagaaaca tctcgcaatt caatcattaa aattcctcct
    18841 tatctaaaaa aagcgtcctc aagaaaatga aaatgtcatt ttcttgagga cgcttttttg
    18901 cgtgttacca cctcatgttc aagctgtgtt cacacacaac tcctcttcaa gtacgatcat
    18961 actctagcaa gatatcggtc gcagccgtca tcgccttaca tctatgctcg ggatgaattg
    19021 ctcgatggcc attttccaat agtcgttcct tgcttcttct cagctaccga agctctctgt
    19081 caaaggttct tctatcgtac ttaccatttc aatgcaaata atattcaaaa aaaaagtcct
    19141 tttactaaca taaactatgt cagtaaaagg acgacgtatc gcggtaccac cttttttcgg
    19201 aagtttcctt ccctcaagcc caacatctca aaaagagaga ttggcgttcg ttttaacggt
    19261 cgaacagttc cgaatttgcc tacttatcga caaattattc tccaaggcca ttttcagctt
    19321 ttcttacttg actcttctca ccagccagag cctctctgca aagtttccaa acttactttc
    19381 cttttcaacg aaatcgatat tttgttgttg ggattaatct acatgaggaa aactgaaaag
    19441 tcaagtacga aattcaaatt attttttcta tatttatttt tctgtttttt tatttaacat
    19501 ttcaataaca gttcgaagat cttttccttc cgcaagaagt ttcaattttt ccttttcttc
    19561 gacggtataa tttcggacaa aactggaatc aggtatcaaa acaacgccta atcctgctcg
    19621 tgaagcagct gttgctccta ctaaagaatc ttccactgca aaggctgaac tagcttctaa
    19681 tcctgttcgt tccaaagctg tcagataagg aatcggtgaa ggcttgttct ccgacacttc
    19741 gtctccgaat gtaaacgtgt ccaagtaatc agctaattta aagtgatcca agatatctgt
    19801 tgctcttttc gtaacagtgg tcgtagcaat gcctagtttc atatgacgtt cttttaagtc
    19861 tgccaatatt tcttttgcat atggttttaa gtggatggct ccgcttgtca gctgttgata
    19921 aaaatattct tcacgcttcg ctcggacttc ctgtactttt tcagggctac ctgctgcttt
    19981 cactaaaata tcaaaacttt gtttccagtt ttttccgccc caagcgtcaa cgatttcttt
    20041 tgggatatgg atatcaaatt gctgaaatcc ccaaagccag ccatcacgat acgttttttc
    20101 tgtgtcaact aacaaaccat ctaaatcaaa tattgcacct ttcatctata cattaatcct
    20161 ttccaaaaca atacacatag tttagcacga ctggtccaaa aagaacagaa gcaaagaacc
    20221 agccttctcg tttataagag ttcttggaat cgacggatat agattttctt cacagctgtc
    20281 acaagcgcca gataagcgat gactgtagca aataaccata accagtaatt gccaggaagt
    20341 ggcatcaagc ctaattcagc accaaaactt gtaaaaggaa ggacagaacc gacaataatt
    20401 cctaatgtag tgataacaaa catggcaaat gttgcgttgc tttggataaa tggtactttc
    20461 ggtgtacgca gtgcatggag cactaaggtt tgtgtccata gtgattcgac aaaccatcct
    20521 gcatggaata atgcgatgaa aatcactcgc tgtgaactat ccaatgtatg gaaacttccg
    20581 cccactacag caggacaaat aacaaaatac atcaacaagt aagtagtaat atcaaatatc
    20641 gaacttgttg gaccaaagta aaccatgaat tccccaatac tagatgcatt ccattttttt
    20701 ggctcgtcca aatattcttt atccatccga tcccatggca aggaaatgca ggaaacatca
    20761 tagatcagat tcaaaaacaa aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta
    20821 cttgcaatca gcacggaaaa catattgcca aaattagaac ttgctgtagc cttgatatat
    20881 ttcatgatat tcccaaaagt ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa
    20941 tctttttcta aaaggatcac gtctgcagat tctttcgcaa tatccacagc tgtatctaca
    21001 gaaataccga tatcagccac tttcattgca ggagcgtcat tgataccatc tcctaaaaat
    21061 ccgacagtat gaccattttt acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc
    21121 aattttacga atacttcatg gtcttcagct acttgtgcta attcatgatc actcatttcc
    21181 gagattttct caccggtaac caattcattg atctctagac ctacttgacg gcacactgaa
    21241 cgtgtaacta attcgttgtc cccagtcaga acttttacag ctacaccatg ttctttcaga
    21301 gctttcaacg cttgtttcgt tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc
    21361 aaaaccatgt ctgactcatc tttgactgaa aattctccaa cgacgcttgg attggtcttt
    21421 tgtgcgacac caatcactcg taagccatct tcattaagtg actgtacttt tgcaagtacg
    21481 gcttcttttc gttcttttgt taatggtgta acagtaccgc caagatcaat aaatttagaa
    21541 atgttcagca tttcttcaat cgcacctttt gtgatcattt gtgttttacc agctggatca
    21601 gcaaccacaa cactcatacg acgacgttca aaatcaaaag gaatttcatc cactttttga
    21661 taattgatct tatctgtatc taaagtttct gttgcttcat caatgatagc cacatctaac
    21721 aaatttttca gccctgtttg atagtagcta tttagatacg catggcgcaa aacacgatca
    21781 tcttcatttc cttcacaatc catatgatat tcaagaatga ttttatcttg tgtcaatgtc
    21841 cccgttttat ccgtacataa cacatcgatt gcaccaaagt tctggatggc attcaaattt
    21901 ttgatcactg ttcctttttt ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg
    21961 atcattggaa gcatttcagg tgttagacca actgctacag atagtccaaa aagaaaagct
    22021 tctagccaat ccccttttgt aagcccattg atgacgataa ctgctggagc catcaatgac
    22081 ataaatttga tcagtagcat ggatgtttta tggatgccta attcaaagct tgattgaatt
    22141 ggtttttcag atagcgtttc tgctacatga ccaaatagtg tttcatttcc aacagaaaca
    22201 atgattcctt ccgcacttcc actgatgaca ttacttccca taaagacaag attttcgtaa
    22261 cttgtttcgc tttgctgatc gtcttcacat actttcgcat gcttttctac tggatagctt
    22321 tcccctgtca atgctgcttg ggaaatgaat aaatcctttg cggacaataa acgcatatcc
    22381 gctggtatca tatcccctgc tgaaagccga acaagatctc cacagacgat ttcttccata
    22441 ggaatctctg tgtactttcc attacgtttt acagctgccg ttactttcac aagtgatttc
    22501 agtttttcag ccgcttgatt ggaacgtacg gactgaatca acgtcattgt cccgctgacg
    22561 ataaccatag tgaagataat ggctacaccg aaaagatctt tatctccagg agctgctaat
    22621 acatattccg tgatgaatga aataattcct aatgcgatca acactaatgt aaatggcgtg
    22681 atgtatgctt tcaatatttc tacaatgaat ggtgtttttt tgccatatga aatcgcattt
    22741 tcaccgtaat tttctcttga acgttcgatt tgttcttctg tcaatccatt tgctgatgca
    22801 tggaatagtt caaataattc ttgtttcgtt tttccagcaa aagctgcata gattttttgt
    22861 gtatctttct ttttttccat ctcaatctct cctttgtgtc caaaagacag ctgttctcgc
    22921 tgcttttggt ctttagctat tcatcggatc gatagagaaa aaactgaaag gggctgagaa
    22981 cagatagata atagccctgg cctttcctct agatccttgt ttttcgaatg actgtcatcc
    23041 atttgttctc atctccttta ctaagtaatt ttaattcatg acttccaatc atttttcgaa
    23101 tagttatttt tccaattaga ccacctcctt tttatttttc accgatgggg atttgcggca
    23161 acaaaaaaac ctctcgccaa atactcagcg aaaggtttga aaaagcatat taaaaaaagc
    23221 ttctcagatc tcatcgttga gttttgacac tgtacaacgt aaaaagcaga tacctgcttt
    23281 tagctacctt atgaaaagcc ttatctcgac aattcctgtt ctacccattg gcgtctctcg
    23341 acgtttttgg gcagtagcct atgtttgtat aggagcctca cctaacaaat cttattcggt
    23401 tttccttcgt taatataaca actaaaaaag aggatgtcaa atcaattttc catgtttttc
    23461 atactttttt taaaatacac acgttatatt cctgtctcac gcggttttat ttgtctcatt
    23521 ttccacctcc cgctataata gaaatatagt aggaaacgag gtgctgttat ggaaagaaca
    23581 cttgttatta tcaagccaga cggcgtgaga cgccatttag tcggaagcat catccaacgt
    23641 tttgaagcaa agggactggc cattgctgaa atgaagtttg aaactatgac tcctgaatta
    23701 gcaaaagaac attatcagca cttgactgag cgcagttttt ttgatgaact gatcgattac
    23761 atgacttccg gtcctgtcgt ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc
    23821 cgtaagatgg ttggagcaac aaaagctgcc gatgctgttc caggtacgat ccgaggagac
    23881 tacgcgctgc cggggactga aaacatcatt cacgcttctg attcccgtga tgctgcggta
    23941 aaagagatcg cccgcttttt cccgccattc gatataaaca aaccgtctga aaaagcggcg
    24001 aagtgataca aattctaaac tgctggtcta gttgctgggt tcagcaatgg ccagcagttt
    24061 tttctctaag gacctccgtt gattctttcc taacatatat tcgagcactg tgaagaattc
    24121 ttggtagtct ttcgtctttt cttcccctaa agcagcagaa atcaatcttt gataataatc
    24181 tgccatcaat gtttcataga tcactgtact tctttcttta tgtgctaagc tccgtttcat
    24241 atcttcattc acactacgtg cctttttcaa ccgaccattt tctatataaa acgaagtcag
    24301 gttcattaaa aaagtaaaga gatactgctc atgattgttc aactgtgcta atcgtcgcat
    24361 tctttttttg attttcaaat aattgttctc aatgaaccga tcactgaata tccaaggaaa
    24421 atcagctaga atagatagtt caaattgacc atagtctggt atctgcatca agtattcttc
    24481 aatgattcgg cattcataac gtaaaaattc aaattctgtc tcagatactt ctgttttttc
    24541 gaccatgtga agattcaaaa ccaaataata cagatcttct gttttcttaa aatgcctacg
    24601 gttattgctg atctctttat aaggatcaaa gctgtcttct tgtacccgat ctagcattcg
    24661 tctttttttc tttaacttta ttggttcatc cagctggttt cgcatccatt catattctgc
    24721 aagtgtcatg ttcattcgct ctaaaaatga tctcaatgtt acaaatgaca tttttttcac
    24781 tgaatttgtc tcaatcccga ctagagaagt cttaaaacag attccttccg ctaattggga
    24841 ctgtgtcaac ttcttgttga ctcgaagttt cttgatcaat tcaccatcat acataatagt
    24901 tctccttttt aatttaaatt tttccacatt caataaatag atacgttaaa aaggaaaaat
    24961 acacgaaaaa aataaaaaaa tgtttttttc ataaaaaata ccaaccttca agcattagaa
    25021 tgttgtctaa ctactgaagg ttggtttgat tgtttattga ttattttttt gctgttcaac
    25081 gtctgttctt gttactggat ataagttggt tgttgtttta ttaccatttt ttgaaaagac
    25141 acttgtcgaa tgttcgcctt tttgcttttc gattgcttca ttacgttccg cttcattttt
    25201 atagtcatat tttttcggat caatcgtctc aaatccgttt ggcacataaa accgcaagag
    25261 attttcctga ttcagcttat ctgaaagact taacgcagtc ttgacttttt cctgatcttt
    25321 ggagatttcc tcatcgattc ctgctgcttt cgtatcgatg atttctcctg tctgattatt
    25381 gtaagctttt cctcctagga cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc
    25441 aactacttga tcatgctgag aagataataa gtctgtacca aaatgaagat aatctttatc
    25501 atcgattccc aatagatgta gtaatgtcgg caaaacatcg atttctccac cgtattcatg
    25561 attgactgtc cctttagtat aaccaggcat atggatcatc aatggcactc gttgcatctg
    25621 tgcgttgtca aattcatccc atgtatctgg atctttacca agagcgcttg ctaaatcttt
    25681 attatctgta ttcgagatcc caaaatgatc cccgtaaatc acaaagattg aattttggta
    25741 aataccagat tttttcaaat acgtaaaaaa ttgttccaat gattgatcca gataatgtgc
    25801 cgttcgaaca taattatcta ctgtgctatt gccagtattc aaagatggaa agtcgaaatt
    25861 cttgtcatcc gtataataag gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg
    25921 ctgttgtaga tgctcaagat attgggcaga ttctctgaat aaatctttat ctaatatccc
    25981 ataacctaac gtttcgtcgg attcatcaaa ataagatcga tcaaagaaat tgtcataacc
    26041 taagttttta tatacatgat ctctattcca aaaactagct acattcccat ggaaaacagc
    26101 gcttgtatag ccttgctttt gtccaaggat cgctggtgcg gcttgaaaga cattgtctga
    26161 cccaagttct gtgaataatg agccttgagg taaaccaaat gtgccagttt ccaacatatt
    26221 ttcagcatca cttgtctttc cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt
    26281 tgcttgattc tggaaaatac tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat
    26341 attaatcaaa aactgctgga aactttctaa atggagaacg atcacatttt tccctttagc
    26401 gatcccaaat gtttcgggat ttggctctgc ataatgtttc tttgtataat ctaaaacttc
    26461 atcaatccca ttactgcttg catgcgcgcg aacactgcta gtcattcctg ttttgattcc
    26521 gtcataaata gtatacgcat cgattcctag atatttgaca atatatgaac ggtcaaatgt
    26581 tcgctgcagc aattgtggac ggttggcttc gctcagcgct agatttcctg aaaaaataag
    26641 ccctgaaaga caggtaacag cgatagccat cggtttgccg acagggttgc ttttgatagg
    26701 tattttcttg accttcaacc aaaccagtag cccaatgaac acagcgatat ccagccagta
    26761 gatgacatca tggggcttca tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt
    26821 cgaaaagcct aatacagatt tgactgtaat gaaatctgtg aattcccgat aaaaaataat
    26881 attcaagtac aacaaaagcg tatttaaaat atcaatgatc aaaagaatag ctaacgctgg
    26941 ctttggcttt ttaatataca aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt
    27001 gatccacaag ataaagtact ggatcgtacc ttccacacct aatgaaaaat ctgcatagta
    27061 ggcaatgatc gtcttcagcc agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt
    27121 ttggcacaat tttttaagtt gtgtcacgta tatcacttct ctctattcac attttttctt
    27181 attttgctcc tgaaaaatga aaatttccta aaaaagttgt tatcttaaga tttttttatt
    27241 ctttctaaag attattccgt ccacttttca cagcataaaa ccgctgcgat gtaagcgatt
    27301 aaactaatga agagataaaa gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc
    27361 agtttctcgg tattcgtaca aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta
    27421 tttcttgatg caggacagct ttttcacaac ttcttcctta aacggtgttc tcctgccaac
    27481 tcctcggcca caagtcacaa tatttataaa acatgagaag ctgttttttg aaattgttcc
    27541 ttgtggctca gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct
    27601 gaccctcggt attagattat gtcaggacgc taattttaca acctcttggt aaacggtgtt
    27661 caaaagcttg catctgcggt aataagccca accaaacgaa aaatataagg agctattttt
    27721 tgaaattgct ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt
    27781 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    27841 gccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct gtttttcatt
    27901 gtccatagga ttgatattgt ccttgtgatg actcattcgt aataattgag gcttgatttt
    27961 cttcacttcc cgttatcgtt tgtccactgc cagaatctga aggacttgtt cctgtcgact
    28021 cgtttttttg agaagagaca gttgttttct tgacttgttc atttgtcttt ttccattccg
    28081 ctaaacgatc ggcgaccact tttttgtcta ctttttcaga ataagcggtg atcacttttc
    28141 catttttcaa acgggctact ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct
    28201 tgtctttaaa ttgatattgt ctgagagaaa tcgcactaag tacgtcatcg atattaataa
    28261 attgattttc ttcttttcct tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg
    28321 cagttatttt atactccgcc caccaataag ttgagaattc ttttttgaaa ttatctactg
    28381 attctttgct tgctacgatt ttttctgttt ctttctgagc aagatctgct gtcatttttc
    28441 ctgaaaatgc tgcggataaa ataagcagta agaaaaaacc gaataaccaa aagagaagaa
    28501 acggtctttt tttcctcatg ctttcctcca ctcctattta attttgacta ttctattata
    28561 tacaaaaaat gaaatagaaa gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct
    28621 ttttcacaaa atggtaagaa tggagaattt cacgcatttg ctctcaacta ttctttctta
    28681 agcctcaaat ccgtttttat ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt
    28741 tctttttcca tcttgtgcta agtctactga aataaagcca tatcgattct tatatgcatt
    28801 ggtccacgac cagttatcca tacatgtcca catatgatag cctaaacaat tggcaccttc
    28861 ttgaatggct tgatggacat atttcagatg agattggaca aattcgatcc gataatcatc
    28921 gtggatcatg ccatcttctt tgataaatcg tgcttcgttt tctacaccca taccgttttc
    28981 agaaataaag cagtcgatat ttccataatg atctcgtaca ttgatcaaaa tatcatagat
    29041 tccttgttca tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata
    29101 attgtcgaaa taatcctcag gcattggacc gtttttagta tcgattggtg tttcttttgc
    29161 ttttacccgg cgtggctggt aataattgat acctaaaaga tctactgtat tttctttgat
    29221 aaccgtcaaa tcctctgcat ggatcacagg catgagatca agttctttta ctatctcaat
    29281 caattcactt ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc
    29341 atctgctatt tgcgctgctt taacatcttc agggtttgat tcgtctcttg gatagcttgg
    29401 tgtcagattc aagatgatac caataacacc accttgctcc atttcatggt aagatttgat
    29461 ggctaacgct gaagccaacg cttcatgata gccaacttgg atcgcttttt tcatatctga
    29521 ctcttctgga taatgccatc gatataaata acctgcttcg actggaacga ttggttcatt
    29581 atgagtaaac cattttttta ctttcgaacc aaacaattca aaacaacttt tggcatattt
    29641 ttcataggct tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg
    29701 catatcgaaa tgatacagat tcacaaaggg ttcgataccg ttttcaatca actcatcaaa
    29761 ataacgatga tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa
    29821 acggctccat tggatagatg ttcgaaatga attatgccca gtctgtttca tcaactgtac
    29881 atcctcttga tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa
    29941 cttttgcggt tcttttttgt accaataatc aaaaatgttc tctccttttc catcgcctga
    30001 aatccttcct tctgtttgtg ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa
    30061 ttgatatttt ttcatcaaag cacctctttt tcttttttag ttcgttttag ttgattccat
    30121 gtatgatatt ttccatcaca atcacttttt cattttcttc ctcttctttt ttgattcgtt
    30181 ttaacagctg ttctgctgct cgttccccga ttttatctgt tggtggcgtg atcgtgattt
    30241 tcggaaggat actcagatcg tttttatcta aataatcata gtgactgaac gcaatatcct
    30301 tcccgattct gacaccttct tgattgaaac attcgatggc accaatcgac atattgtaat
    30361 tgctgacaaa aatagcagtt gccccttttt ctaataatgc ttttgccccg taatagccgc
    30421 tttttctaga atagtctcct gtaaagacct ctaattggat cgatggatca ttttggaccg
    30481 cttctttggc tcctgctaaa cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa
    30541 atccgatttt tttatgtcct tgttcaccat gatgagcgat cacttttgct acactttctc
    30601 gatcattcac taatattgta tcaatacctg ctggtccttc tggacaattc aatgcgataa
    30661 ctggaatact catttttgtg agcatttcaa atccagacca ctcttcttcg gataagaaga
    30721 caatcagccc atcgatcaca tgcttcatca ggtattcaat tttttgttca attttttctg
    30781 gctgatcggc aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga
    30841 tccctgagac aacttccgac ccaaaacggc tggaaatccc gtttatcaaa agtccaatag
    30901 agaagcttcg attattcttc aaccctttag caatgatgtt ttctttatag tccaacgctg
    30961 aaatcgcttc tgctattttt ttcatgttct tttccttcag ttgctgacca ttcaagtaac
    31021 gggaaactgt tcccactgaa acaccagcca tcttagccac atcacggatc gatgccatac
    31081 attctcactc ctgccagtta catttgattt ctctttcttg ttgttcgatt atgatgaccg
    31141 cattctttgc atagtgttgc ttgtcatcaa tatccatata actaaagtct tcagtagagc
    31201 aacttcctct cactattaat tatacttaaa caagtatttg aaccggttca tttttaatat
    31261 agcatttttt taaaagattg caacactttt ttaaaacgct ttctgaaagt ggcggatttt
    31321 ttgccttttt aaaattttat tctaaaattt actctatttg tgggtgtttt gtgcgttttt
    31381 ctcttccatc cgtttgaagt tttacattcc aatggtataa tttctttatc ctatcaaatg
    31441 ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat
    31501 tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt
    31561 ctgtcttaaa agcggaaaaa gcagatacca ccaacagtgt atatacgcta aaagtacgta
    31621 aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac
    31681 gcacgtatca gacacaagga actgattctt ctgaatcatc tgaagaaaaa acagtgctga
    31741 cagatatcaa gatcactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa
    31801 aagaaacaac agtcatattt acaaaaatcg gtaattaccg tttgaaggat gaggatggca
    31861 acatctacag tctatagtgc cgtctgttgg atcttctttt gtttctcccc ttcttttctg
    31921 ctatagtaaa gaagcaagat gattgtaaag gagacgaaat ggatgatcga tagtcctgat
    31981 tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgacca aggaagcttg
    32041 agcatatgcg acaactgtca caaactagtc aaagattcat ttaaaacatt tgaatcttat
    32101 cctgttcaaa attatgtaaa aagtagaaga agataaacaa atccaagcaa aactcactat
    32161 ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc atcagtaaaa
    32221 caaacagtgg atcatttcag tttacaaatc aaagagtaaa gatttattta tttcaaatat
    32281 ttgaatataa aataaccaaa cgatagaaag gcttattcta catcacgtag ta
//
LOCUS       DAZKNM010000013        29870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000013 DAZKNM010000000
VERSION     DAZKNM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.013"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..597)
                     /locus_tag="ACG8M9_000513"
     CDS             complement(19..597)
                     /locus_tag="ACG8M9_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0377312.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(674..1204)
                     /locus_tag="ACG8M9_000514"
     CDS             complement(674..1204)
                     /locus_tag="ACG8M9_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0377313.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1208..2068)
                     /gene="rlmB"
                     /locus_tag="ACG8M9_000515"
     CDS             complement(1208..2068)
                     /gene="rlmB"
                     /locus_tag="ACG8M9_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0377314.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2061..2456)
                     /locus_tag="ACG8M9_000516"
     CDS             complement(2061..2456)
                     /locus_tag="ACG8M9_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0377315.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2453..3865)
                     /gene="cysS"
                     /locus_tag="ACG8M9_000517"
     CDS             complement(2453..3865)
                     /gene="cysS"
                     /locus_tag="ACG8M9_000517"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0377316.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3862..4398)
                     /gene="epsC"
                     /locus_tag="ACG8M9_000518"
     CDS             complement(3862..4398)
                     /gene="epsC"
                     /locus_tag="ACG8M9_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0377317.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4484..4723)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4720..6180)
                     /gene="gltX"
                     /locus_tag="ACG8M9_000519"
     CDS             complement(4720..6180)
                     /gene="gltX"
                     /locus_tag="ACG8M9_000519"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0377318.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6350..7507)
                     /locus_tag="ACG8M9_000520"
     CDS             complement(6350..7507)
                     /locus_tag="ACG8M9_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0377319.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7541..8911)
                     /gene="radA"
                     /locus_tag="ACG8M9_000521"
     CDS             complement(7541..8911)
                     /gene="radA"
                     /locus_tag="ACG8M9_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0377320.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8974..9501)
                     /locus_tag="ACG8M9_000522"
     CDS             complement(8974..9501)
                     /locus_tag="ACG8M9_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0377321.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9731..10537
                     /gene="proB"
                     /locus_tag="ACG8M9_000523"
     CDS             9731..10537
                     /gene="proB"
                     /locus_tag="ACG8M9_000523"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0377322.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10534..11781
                     /locus_tag="ACG8M9_000524"
     CDS             10534..11781
                     /locus_tag="ACG8M9_000524"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0377323.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11857..12201
                     /locus_tag="ACG8M9_000525"
     CDS             11857..12201
                     /locus_tag="ACG8M9_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377324.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12318..12644)
                     /locus_tag="ACG8M9_000526"
     CDS             complement(12318..12644)
                     /locus_tag="ACG8M9_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377325.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13259..14461)
                     /locus_tag="ACG8M9_000527"
     CDS             complement(13259..14461)
                     /locus_tag="ACG8M9_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0377326.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14578..15093
                     /locus_tag="ACG8M9_000528"
     CDS             14578..15093
                     /locus_tag="ACG8M9_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0377327.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15292..15738)
                     /locus_tag="ACG8M9_000529"
     CDS             complement(15292..15738)
                     /locus_tag="ACG8M9_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0377328.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15785..17263)
                     /gene="msr(C)"
                     /locus_tag="ACG8M9_000530"
     CDS             complement(15785..17263)
                     /gene="msr(C)"
                     /locus_tag="ACG8M9_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0377329.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17617..18246)
                     /locus_tag="ACG8M9_000531"
     CDS             complement(17617..18246)
                     /locus_tag="ACG8M9_000531"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0377330.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18230..18694)
                     /locus_tag="ACG8M9_000532"
     CDS             complement(18230..18694)
                     /locus_tag="ACG8M9_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0377331.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19181..20449)
                     /locus_tag="ACG8M9_000533"
     CDS             complement(19181..20449)
                     /locus_tag="ACG8M9_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0377332.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20680..21417)
                     /locus_tag="ACG8M9_000534"
     CDS             complement(20680..21417)
                     /locus_tag="ACG8M9_000534"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0377333.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21484..22053)
                     /locus_tag="ACG8M9_000535"
     CDS             complement(21484..22053)
                     /locus_tag="ACG8M9_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0377334.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22181..23053
                     /locus_tag="ACG8M9_000536"
     CDS             22181..23053
                     /locus_tag="ACG8M9_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0377335.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23395..24267
                     /locus_tag="ACG8M9_000537"
     CDS             23395..24267
                     /locus_tag="ACG8M9_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0377336.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24264..26222
                     /gene="nagE"
                     /locus_tag="ACG8M9_000538"
     CDS             24264..26222
                     /gene="nagE"
                     /locus_tag="ACG8M9_000538"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0377337.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26554..27405)
                     /locus_tag="ACG8M9_000539"
     CDS             complement(26554..27405)
                     /locus_tag="ACG8M9_000539"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0377338.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27528..27995
                     /locus_tag="ACG8M9_000540"
     CDS             27528..27995
                     /locus_tag="ACG8M9_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0377339.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28258..28740)
                     /locus_tag="ACG8M9_000541"
     CDS             complement(28258..28740)
                     /locus_tag="ACG8M9_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0377340.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28840..29703)
                     /locus_tag="ACG8M9_000542"
     CDS             complement(28840..29703)
                     /locus_tag="ACG8M9_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0377341.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8509 a         6330 c         5423 g         9608 t
ORIGIN      
        1 cattaagatt tttctaaatt attcctcatt ataaaatttc aagtgttttt ttattttgag
       61 ttttactctc ccgtaagcgc tcattacttg cttgtgatcc attgctaaac attcagccat
      121 gtctacaaaa tcaacgcctt gcatataata atgaaaaatt ttagcttcta tttttgaaaa
      181 ctggatttcg caattttcga ttgcttcttt taataaaaga tgctgaattg ctaaaggttc
      241 ttgcaatgtg atctctgcta aaaagtctgg tccttctgtc tctatttttt ggtcgattga
      301 gacttcatct ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca gagaatagat
      361 ttgattgctt ataacgcttt tataataact tccaaagcta acacctttgc ttttttcgta
      421 agttctcaat gcctgatata aagcgatcct tgcttcttgt tcccaatcct cagaaacata
      481 atttcttaaa taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag
      541 ggcagcaaat gcttcttcgt caccattacg tgcagcgcgc aataactcct cagacataaa
      601 ataattcctc cataaattcg tttatggagg aatcttatca tcaatgattc atcgaactga
      661 ttagaacgat atgttattta ttttttctca ttaaatcgtc caatttttgg gacaattttt
      721 ttaactgctc ttcattccat ggagaattac gtcggaaatc aataaaatgc atatctttat
      781 tttcttgggc tattgttttt tccgtttttt ttatcgtctt atataattca taagcagaag
      841 ttcgtaatgc tccttgggaa aagatcatcc actgttctgc taaatcgctt gtcgccactg
      901 tcacttgtgt caaacggtca ttcagctgac cagcgattct ctcgatataa ctgtcagccg
      961 tttcgtcttc tttcgtaaaa acaacatcca actggtattt cttataattt tgttggatgc
     1021 ccggaactag ttgggcatcg aagacaacga tgacttccat tccttcatat ttcgcataat
     1081 ttgataaacg atgtaaaaga gcttctcttg catccgccat tttgtcctgc tttttaagct
     1141 ggacaagttc cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac agctgttttt
     1201 tcatggtcta ctccccctga cgttgggaaa aagcctgata cataagcaaa ccagcagcaa
     1261 cactggcatt taagctctgg acgtgcccgg tcatcgggat cgtcagcaat tcatccattt
     1321 cttttttcaa tcctggactc attcctttgc cttcattacc aatgactaaa gcaattgatc
     1381 ctttggcatt ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc
     1441 ctgtttcttt gagctgttgc acagcttgta ccaaattcgt cacgcgtgca acagggacat
     1501 gttcgactgc cccagtggaa gtctttgtta cgataggtgt gattccaact gctcggtgtt
     1561 ttggtatgat cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat
     1621 tgtgtggatc ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt ttctcttttg
     1681 ttcgttcaat cagttggtca agggataaat attcataagg tgtaatcgca agaacgactc
     1741 cttgatgcac gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc catttgactg
     1801 gcacagcttg ttcacgtgcg atttctttca gttcttctac tttttctcct cgtgaatctt
     1861 cttgaagaaa gagtttattc cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc
     1921 caaagacgaa attatcttct aatgcttctt cattttgatc agaaactgac tgatttcgtc
     1981 cagtttgctt tttatttgaa tatctatttt gttttttgtc ttcaaatcca cgtttcgatt
     2041 gatgtttgtt tgttctttct ttacccattt ttttcaccaa ctttctgaat acaccagcgg
     2101 atcaattctt ccatccgctc ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg
     2161 aaccctgttg cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta
     2221 gcatttcttc ctctgcgata ataaagttct tcttcttctg tcaaaagatg ttcttccagc
     2281 atttgctgaa tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc
     2341 agcttattgg gttttgtttc tccttgatca atcaggtggt cacgaacata gatttcgtaa
     2401 atcgcatctc caacataagc caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt
     2461 ctcttctcca tcgggttcct tgcggtgtat cttctaataa gatccctttt tctttcaaca
     2521 aatcacgaat ttcgtcgctt cggacaaaat cacgattgct tcttgcttga tttcgttctt
     2581 cgatcaattg ttcaatctct tcatctaata actcttctgc tgtaaataaa ataccgaaga
     2641 tcgaaagcca ttgcgagaat aaagcttctc cttgtgccaa gacagcggca gaaactttcg
     2701 gctggtctag ataagtgttc agccacttag caagctcata gaccactgtg atcccatttg
     2761 ctgcattaaa atcatcgtcc atttcttcga tgaatcgctg ttctaaattt tctaacaaag
     2821 cgattttatc cgcatcatct gccaaattct ctgaggcagt ttcttggcgg aatttcaaat
     2881 tgtcaaacgt attcttcagt cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac
     2941 tgtagcggat cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat
     3001 caacgttttt gatcatttca tgtacagtga tgaaattccc tagagattta ctcatttttt
     3061 catcgtcttc gccaatcgtt acatagccat tgtgcatcca gtaattagcg aattttttcc
     3121 ctgttttcgc ttcactttgt gcgatttcat tttcatgatg aggaaattct aaatcttgcc
     3181 cgccaccatg gatatcgatc gtttctccta aatgttttgt tgccataacg gaacattcaa
     3241 tatgccaacc gggacgtcct ttgccccatg gtgaatccca agaaatctcg ccaggttttg
     3301 cttgtttcca taaagcgaaa tctaatgggt cttctttttg ctgctgttct tcccctgtac
     3361 gctgacttgc tccgacttcc agttcatcga tggattggtc acttaatttc ccataatttt
     3421 caaatttacg ggtacggtaa tacacatcac ctgaagattc ataagcatag cctttttcaa
     3481 ccaacacttg aataaatgct aaaatatctg gcatatgatc catgactctt ggatgcagtg
     3541 tagcaggttc gacattcaac gctttcgtat cttcttcaaa tgctcggatg aaacgatcag
     3601 caacttctgg tgctgtaata cctaattcgt ttgctgtacg gatgatttta tcgtctacat
     3661 ccgtgaaatt cgatacgaaa tcgactttat agcctcgata ttcaaaatag cgtcgaattg
     3721 tatcgaaagc aatggtgcta cgagcatttc cgatatggat ataattatac acagtcggtc
     3781 cgcagacata cataccgaca tgtccctttt gtatagggat gaattcttct ttttttctgg
     3841 tcaatgtatt gtagattttt atcattctga tctcctcact ttttctcctt tgattcgaac
     3901 gacttttgct ggtactccta ccgctgtagc gtcgtctgga acatctttca acacaacggc
     3961 acctgcaccg attttggcgt tttttccgat cgttaccggt cctaatatct gcgcattcgc
     4021 agagagcatg gctccttgtt taacagttgg gtgtctttta cccgtttctt ttcctgtgcc
     4081 gcctaatgtt acgccatgga ataaaatcac atcgtcctcg atttctgcgg tttcgccaat
     4141 gacgattccc attccatggt cgatgaacac gccagttcca atcgttgctc ctggatggat
     4201 ctcaacacca gttaggaaac gccagaattg agcattgatc ttcgccaata aaaagagccg
     4261 gtgctgatat aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt
     4321 caaaacgatc tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg catcaatcgc
     4381 tcgggtaaac caactcatga ctttccactc ctttcgtaag aattttatgt aggaaatcat
     4441 cttcttataa agaaaagaag cgccttttaa ataagtttcc ttatcaaaag acgctttccg
     4501 cgtggttcca cttttcttca taatcaatga ttatctctga cagctaacgc ctgttatacg
     4561 ttcttggcta ctttgtttcc tataaaacag tttttcctgt tttctgtgtt tcgccaagac
     4621 cactcccaga ggcatttcgg caaccattca ttgatacctt gcaccacccg gtatctctct
     4681 tgtaatgatc agctgcttac ttttttctgt tcatcgtgtt tagttcagta cttgatttaa
     4741 atgtgctaat gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg
     4801 catttgtcct gatacagcta cacggattgg catgaaaaga ttttttcctt ttacgccagt
     4861 ttctttttga acagctttga tggcagcttt gacagttgga gcatctacta cttccatttg
     4921 ttctagctgt gctttgaatg cgtggagaac agtaggaact gtttcaccag caagtacttc
     4981 ttttgctgct tcattcaata caggatgttc attgaaaaat agcgtagaca gatcaacgat
     5041 ttctgcagca tagctcattt gaggttgata caggctaacg atcttcttca cccattcgat
     5101 tgtcttttca tctgggtttt ctgctacacg tccatcagcg attagataag gcagacacat
     5161 gtcagtaaat gcatctagat ccattgcttt gatgtaatgg ttgtttaccc attgcagttt
     5221 tttcgcatcg aaagctgcgg gtgatttact taggcgtttt ggatcgaaca ttttgatcaa
     5281 ttcttggtgt tcgaagattt cgtcttcgcc cacaggtgac cagcccaata atgcaataaa
     5341 gttgaacatt gcatctggta gatagcctaa ttcgcgatat tgttcgataa attgcaagat
     5401 tgattcgtca cgtttgctta gttttttacc tgtttcactg ttgatgatca atgtcatatg
     5461 tccgaaggtt ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt ttggtgtatt
     5521 ggcaatatgg tcatctccac gtaaaacgtg agtgattttc atcaaatgat catcgaccgc
     5581 aacagcaaaa ttataagttg gcataccatc gcgtttttgg atgacaaagt cgccaccgat
     5641 attatcggat tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt attctgtatt
     5701 gcgaggcaca cggaaacgta caacagattc tagaccttgt gcttcttttt ctgcttgttc
     5761 ttcaggtgtc aagttcgcac atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg
     5821 tgcttcccgt tctgcttcta attcttcttc tgtgcaatag catttgtatg ctcgattgct
     5881 agcaagcagc tgatcaatca gcggttgata gatttcttta cgttcagatt gacggtaagg
     5941 gccgtattct ccaggatttt caggagattc atcccaattc attcccaacc aagcaaggtt
     6001 ttccaactgg cttttttcgc catcttcgat attacgtttt tggtcagtat cctcaatacg
     6061 aataataaag tctccatcat tgtggcgggc aaacaaataa ttgaataatg cagttcttgc
     6121 atttccaata tgcaggtgcc cagttggact tggtgcatag cgtacgcgta ctttcgtcat
     6181 tttcttcctc ttctttcaaa ctagactttg actaacagca aaaattccgt cagttacggc
     6241 ttctcaaata tcatttattt aaagccactc agcacaaatt ttaccttttc taaggtaaat
     6301 agtaaagcca actggttgaa tttccttacc agttggctga atttcttttt tattttgatt
     6361 gtttagtctt tttctctgtc tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt
     6421 tagcaaagat catgcgtcca gcagctgttt gaagggcact tgttacaacg acttcgatac
     6481 gttcattcat gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg
     6541 ctactccttg ttggcgttct gtcccggctt ttactactag aacattcatc gtttcaccag
     6601 ggatgacgac tggttttacg gcatttgcca acgcattgat attaagaaca ggtacatttt
     6661 ggaattcaga gactttattc aagttatagt cattggttac aatgacacca tctaaaagct
     6721 tagccaattt gatcaatttg ctgtctactt cactgatatc ttcaaagtct ccatcgtaca
     6781 tttcgacaga tataccctct tctttttgca aggcatttag gatatctaat ccgcgacgac
     6841 cacgcacacg tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa
     6901 aattagggat catcaaggtt ccttctaaga atccagtctt tgtaatatca taaatccgac
     6961 cgtcaatgat cacgcttgta tccaagattt tatatttatg gaaattttct cctgattttc
     7021 gttctaacag ttgactctca acttgactag ctgtttcttg ctcgattttt ttgcttcgtg
     7081 aaccaaaaac tttcttccat tcttcgattc gtgtcgttcc cattctcaat cccagatagc
     7141 ctaagatgat catcagtaaa atcggcaata cactattgac gaatggaatt tccatattgt
     7201 aaagaggggt cgagatcaca acacctaaag tcaatccgat gatcgcaccg atcgcaccga
     7261 agagaagata agtcaagctg acctcactta gtttttgttc tacttgtttg ataatagctg
     7321 agatatgttt ggccaacagc aaagataata aaaagaaaat aagtgcgcct aacaggctgt
     7381 tggtaaatgt attattcagc catccgtttg actgttgacc gattgcctgc caagctgagg
     7441 gtaaaaatga aatacccaag cttgcgccga caatgatcat cagcaaagta atgacacgtt
     7501 tttgcatcgt ttttcctccg tttcgacaat catttattag ttagcggaac acacgcttca
     7561 acgtttcagc caaagttgct actccaacta tctcgattcc ttttggcgct tcccagttac
     7621 ctaagttgtt tttaggtagg tatacttttg tgaagccgag tttttggact tctttgactc
     7681 gttgttcaat atgactgaca cgtctgattt ctccagtcag tccaatttca ccgatgaaac
     7741 attccgttgg ctgtgttcct ttttctttat agcttgaagc aatactgaca gcgatagcta
     7801 aatcaatcgc tggttcattt aattttaccc cacctgcagc ttttaagtaa gcatcttgat
     7861 tctgaagaag caacccagct cttttttcca gcactgccat gatcagagat acccgattga
     7921 aatcaagacc tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg actaatgctt
     7981 ggatctctac caaaattgga cgtgtccctt ccattgccac aacgatagca gagccagttg
     8041 caccgtctag gcgttcttct aagaaaatct gtgaaggatt agcgacttct tccaatccat
     8101 gctcatgcat ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt tttactgctc
     8161 gcaaaatccg aaaactatga tgttgttcac cttcaaaata taacaccgta tccaccatat
     8221 gttctaacat tcttgggccg gcgatcgagc cttctttggt aacatgaccg acgataaaaa
     8281 tggctatccc atttgtttta gcggttttga gtagttctgc agtggtttct cttacttggc
     8341 tgacacttcc tgccacactt gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga
     8401 taacataatc aggggagata tgttcgatgg cacgactgat ttcgttcata tctgtttcag
     8461 cgtatagata aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct
     8521 ctgcgctttc ttcgccggat acatataaga cttttccgcc tatggctgct aattgctgag
     8581 aaacttgtaa cagcaaagtg gatttcccga tacctggatc gccaccaatc aagaccatag
     8641 accctggcac cacacctccg cctaacacac ggttcagttc ttctaatttt gttttgatcc
     8701 gaggttcttt tttagggacg acgtctgcga tttttgttgg ttttgctttt tctcctgtca
     8761 aacttgtccg agttctgcgg tccgttgtat cttgttcgat ttcttcgacc atcgtattcc
     8821 attttccgca atttgggcag cgtcccaaga acttaggcga cacatacccg caattttggc
     8881 aaacaaattg tacttttgat ttttttgcca ttgtaccgcc tccttttatc ctaatcatga
     8941 gacattaaat caagcttaag tatctttaac gttttattta ctggaagaac caaatccgcc
     9001 ttgacgttga gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa agatcccttg
     9061 gccgatacgc tcgccttttt tgatcactac atccttgaaa ccaaagttgg tgaattggaa
     9121 catgatatgg ccttcatttg cctcattatt gtaataatca ctgtcaatca cacctactcc
     9181 gtttgtcaac actaaaaacc gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa
     9241 ttcatcttct cccatgtaag ctttgattcc agtaggcacg agggttggtt tcaattgttt
     9301 ttcattttct gatacatctt ttttcaatcc atctgcgact gtttccaacc cctttttcca
     9361 gcctgctttc cagatacttg gaatcacgac ctcttctgct gcttcaaaat catagccagc
     9421 agcatgatgt gtagcgcgct gcggtagatt gatattttga tctttatatt tagaaataac
     9481 ttcaaatcct ctttttctca ccggttttcc tccctaaata aactactcct attctaacat
     9541 atccgaggct gtaaaagact ttctttacaa attcttcaag aaattctgct atcttaaagt
     9601 aaaccattaa ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa ctatttttta
     9661 catgacttta ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg atatttgatt
     9721 gaggtgtcac atgcgtaaag aaattaaaaa tgcaaaacga attattgtca aagtaggaac
     9781 aagtacattg atttatccca atggaaatat caatcttgcg gcaatcgatc agttagcttt
     9841 tgtcctttct gatcttagaa atcggggaaa ggaagtgatc ttagtctcat caggagctgt
     9901 aggcgttggt atgcataaat tacatttgtc agaacgtccg actttgattc ctgaacagca
     9961 agctgcggcg gctgttggtc aagccgaact gatgaatatc tataatcaaa gattcagtgt
    10021 atataatcaa cagacggcac agatcctcgt gacaagagac gtggtagatt atcctgaaag
    10081 tcgtaaaaat gtgaggaata cattggaaca gcttttgatt atggacacga tccctatcgt
    10141 gaacgaaaac gatactgtag cagtggatga attagatcat ctgacaaaat ttggcgataa
    10201 tgatcagctg tcagccatca tggcaaaact tgtcgcggca gaccttttga tcgtcctttc
    10261 tgatatcgac gggttttatt cagataatcc gactacaaat cccgaggctc agatgtatcg
    10321 ctatatcgat aaaatcgatg aaaagattct ggcacaagca ggtggtgctg gtagcacatt
    10381 tggaacaggc ggtatgcaga gtaaattaaa agccgcttct aggatatttg aagcaaacag
    10441 cgcaatgatt ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa
    10501 agaagtaggt actttattta aggaggactt gaaatgactg acttgatgca attaggacaa
    10561 caggcaaaag atagcgcgca acaactagca cttatgccta caaaccgcaa aaatgagcta
    10621 cttgttcaaa tggcacaaac catcaaaaag aatcaacaag cgatcattga agcaaatcat
    10681 aaagacttag aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag
    10741 ctgacacctg agcgaatcaa tgccatggct acagagatcg aaaaaattgc ttcgttagac
    10801 gatcctattg gaaaagtaga tgagatgtgg acgaacaccg acggattgag aatcggcaaa
    10861 aagcgcgtgc cactaggcgt gatcggcatc atttacgaat ctcgtccaaa tgtcacgaca
    10921 gatgcagcta gtctagcatt caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa
    10981 gcattcttct cgaatcaatt attagtccaa ttattgcagc aagtactggt cagtgctggc
    11041 gaatctcctt atgccatcca gtttgtcgat gatacttctc atgaaactgc aggacagttg
    11101 atgcgattga cagaatactt agatgtgctg attcctcgag gcggagcaaa attaatccaa
    11161 cgagtcaaag aacaggcaac ggtacctatc attgaaacag gtacaggaaa taatcacgta
    11221 tatatcgata aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct
    11281 tcccgtccat ctgtctgtaa cgcggcagaa acattgctga tccattctga gatcgcacct
    11341 tttttcttgc cagctattga aaaagaactg gtagaacacg gggtctcttt acgtgcggat
    11401 gctcgtgcgt tagaatatct agagacggct gctttagcag aagacacaga ttgggatact
    11461 gaatatctag attatatcct tgctgtcaaa gtggtcgatt ctttggatga agcaattgca
    11521 catatcaatc ggcataatac taaacattca gaaacgatcg tgacagatag ttatgcggct
    11581 agtcaacgtt tcttaaatga agtggacgcg gcggctgtct atgtcaatgc ctccactcgt
    11641 tttacagacg gatcggtatt tggttttggt gcagagattg gtatctctac tcaaaagttg
    11701 catgcccgtg gtccaatggg gttaaatgaa ttaacttcca caaaatatat catttatggc
    11761 gatggacaaa tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt
    11821 tctgtaaagg ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt
    11881 tttatttacc ttatttctct gaatatcgct gcttttctgg gagtcggtat tttatccctt
    11941 ttctttcaat tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc gcagtatttt
    12001 gacaaaatcg ggcaaaaagg gctggttaca cgcttgcaca ttagttacat ggttgcatta
    12061 tgcttattag cgacaatcaa ttactattct tttttcgatc attcaatcgc ttatacgatt
    12121 acgctactga ttgctgggat ttttcactta tcttataaat atcagctgaa taaaaaccac
    12181 ttgaatcgga ctttccgcta aaaaatatcc gtacgataag tagagtgcct tgaagatcgg
    12241 caaaaaactg ccggactttt tagaggctgt gacaaagatt ttgtcacagc ctctttttcc
    12301 atataaacgg cgttcgccta ccagctgcct ggtaaatctg tttcctcttc gttaagaagc
    12361 gaaaatccgt caaatgtttc gccatcataa taacgaagca gctcatccat ggacaagttc
    12421 atcgtttttt ctttttcttc ttccgctgat tcagccagta gctgttcccg atacacctga
    12481 tacataccag ctattttttg ctgcatcgct tttctagttt caccttccgc attgattatt
    12541 ggcaattctt tgatatagcc acgataaatc aaccgatcat ctgaaaattc aatgggtaat
    12601 tcaacgagtg tcaacccgac ttcctgaaaa tattcttcca gcatctatta tccccccatt
    12661 ctttttctta aatgaatttt agcttaccta ctgaaaattt tccagtttca ttattttgag
    12721 aaaaaaaggg aatgtgacaa aaatcgtgtc acatcccctt tttccgaata aatggtgttc
    12781 ttctgccaac tcctcaatca taagtcacaa tattcaacaa acatgagaaa ctgttttttg
    12841 aaattgttcc ttgtgattca gagttaaacg acagtctcac aacctcttgg taaacggcgt
    12901 tcaatcagct gaccctcggt attagctcaa aaaacggtaa aatatgagaa gctatttttc
    12961 cgttttgttt ctctaatact taggataaaa cgctgatttt acaacctctg ttaaacggtg
    13021 ttcaatcagc tgtccttcgg tattagctca aaaaacggca aaatatgagg aactattttt
    13081 ccgtttgttt ctctaatact caggacaaaa cgctgatttt acaacctcct gataaacggt
    13141 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt
    13201 tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct ctgatttctt
    13261 attcttcttc atttgtagca aaataattac ggtgataaat gatacaacat acactgataa
    13321 gaacaatggc acctgcaaaa taaaacgcat agctttcacc aatatgttca ccaatcacac
    13381 ctgcaataaa tggtgctaaa gctccaccga aaaagcggac aaaactatag ctggacgaag
    13441 caacgtttcg ttgcagacct ggtatttcca tagcaatcgt cgtcaacagt gtattgataa
    13501 ttccttggaa aaatcctgct aacacaatgc tgatcggtac aaggactggc atagaagttc
    13561 cagctcctaa taacagcaaa caaataatga ataagacaaa tgctgctaaa attgtccgat
    13621 aagtcgtaaa cttccgctca agagccggtg caacgaaaat cgaagcaata gccagaagca
    13681 caccccagcc aaaaaataca aaaccgactt ctaattcaga atagccaatc aataaaaatg
    13741 gcgcatacgc aagtaaagta aagaatccga aattatataa caatgcgatt agtccaactg
    13801 aacgaagttt atgattgctt aacgctttga caccttccca aaggcctact tttctctttg
    13861 gtttctcagt ttcaggtaaa agtaccgcta gggctataaa accgcaaaac atcaaaaatg
    13921 ctaccccaaa aaatggataa cgccatgaac cactgcccaa gaccccaccg actagagggc
    13981 caacagccat tccgcagcca agtgctgctt catacatcat gattgctttt tctgtgttac
    14041 caactaaaat actgacgatc gttgctaaag cagtagagac aaataaagca ttcccgacac
    14101 cccagccggc acgtaaccca atcagcattc cgatattccc agataatccg ccaagaccag
    14161 cgaatacaac gatgataaac aatcctgtca tcaatgtttt tttcaaaccg atccgactag
    14221 ataagaaacc tgtaaatagc atcaccacac cagtgactaa catgtaacta gtaaataaca
    14281 gggtcgtttc agctggcgta gcgtttaact gtgcagcaat cgtttttaag attgggtcta
    14341 ctagacccac tcccataaaa gcgatcatac aggtagctgc tacaactagc gctgaacttt
    14401 tcccctttgt ttcttcattt gtttgttctt gtatatctat ttgattctcc aatatttcca
    14461 tccctttctc tctattagca tcatacatga tttattttac atgtcaatat gcacatgtaa
    14521 aacgtggaaa ctttactgtg atataataaa gaaaataaga cgagaggtag gatttttatg
    14581 aatactttag cctatatatt attatgcgtg cttttatcca aaccacgttc tgggtatgaa
    14641 ttgaagcagc tgatcacaat tttttgggaa gcccatcaca gtcagatcta tacaacgctt
    14701 gccaagttag accagcaggg ttatgtgacc gttttggaca aagacgagca ctctcaaaaa
    14761 aagatctacc acttgactga tgaaggacgt gctcttgtag aggaatggat caaagaggaa
    14821 actcccgctc ctacgcaaaa agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc
    14881 gataaaacaa cagctactgg actgctattc actagagaac aacagctgaa caaaatcatg
    14941 caaaaaaatc aaaaaaaatt gaatgacctc tcgctagaca agaaacaaga atttggacgt
    15001 tatgttgtga tccagcgcag aattatgctt tgtcagcagg aactccaatg gtgtcagcaa
    15061 gtaagagaac aaatgcagca gttttttgag taagggataa acagaacctt tctagaaata
    15121 tctattgaag agcaaattat cttcaataaa agagcaatac agttggaaaa gaaaaagcga
    15181 tccgaacgac aggccaaaac ctatcgttcg gatcactatt tttctagaac tgcattcaag
    15241 agactatgac aaaaagcatg ccaaggcctt ttttcaaata acagtgttcc atcattcatt
    15301 actttgaatt gcttttagca cacttcttga aagcgggcca acgatgatca actgcaacgg
    15361 taaagcggca atcacattca ttttccaagc attcatataa gcagagaata attgatcagg
    15421 tattccttgt tccatgatca caccgaataa agacatacat gtcaccattc ctaaaaccat
    15481 caagcatgaa atagttaaga tcatttggac cttgctttct ggatttactg gtagtttaaa
    15541 ggcaattttt ttcgccagca caccgatgat gaagacatcc aaaatgaaag ccaccacgaa
    15601 tccaggcact agaccaatca acaaacttga taaatgaaga tcttgatgca gccataaatt
    15661 atacgcgctc atccccaaga ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc
    15721 ttttttattt gtaggcaatc tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc
    15781 acatttaaaa atttctcgta agtacttttt ttattttctg cccttttatt tcataaactt
    15841 catcagccgt acgctcgaca aagtaagaat catgagaagt aaacaaaatc gttcccggat
    15901 atatttgcat tagcttctct aaagcttcga tcgttgccat atcaataaaa ttagtcggtt
    15961 catctaacag caacacatta cttggcttag taaaaagcaa agcaatcgtc agacgggtcg
    16021 cttctccccc acttaatgta cataacggtt tcgtcaaagt ttcctgagca aaccctaagt
    16081 gattcagcaa actacggaca agcgattctg aaaactctgt ttgtctcatg acaaaacgaa
    16141 tgatggattc ttcagatgtc atttgataac ccttttgctg atatacttga aagcttactt
    16201 tcggagagag aaggattcct tctccttgtt tgcggatatg ttctaataag gttgtcttac
    16261 ctgaaccatt ttcaccgaca atcgcgattt ttttacctaa agaaaactga aaatcaccat
    16321 ttaccagtaa tgttctccca cttctttcca attggacatt ttgtcccatg atgggatagc
    16381 ggctgtggag ttcaagagat tttggtactg gaaaacggat ttgtttcatc tgctgcggct
    16441 tggttgtttc tcctattttt tggagtcttc tctctaatgt tttcgcattc ttttggatgg
    16501 ctttttgtac actgtctttt tgtttagagg aagacaaccg atccagtctg atttgttgct
    16561 ttttcttttt actggacact ttttccaact tttccgcttg ttttttcttt tcttggatcg
    16621 atttttccaa tcgttttttc tctttcatga tcgactctgc ttctcgtagc tgagtctttt
    16681 tctccaattc cttttgagtg agataggcac tataattccc cgaaaactct cggatttctc
    16741 cgtcttttac ttcccagatt ttcgatgcta gctcatctag aaaatagcga tcatgactaa
    16801 caaaaaccag cgtaccataa taataacgaa gctcttcaat cagtcgatca atactttttc
    16861 tatctagatg ggttgttggc tcgtctagaa gcatcccttg ttcataagaa gaaagaagac
    16921 gtatcaactc gatctttcgt tcttcccctc cgctccattt ccgctctgaa gtttcttcca
    16981 gtcttaactg gaaatgactg gctaattcca aatttaggtc ctttgcttca gcgagatagg
    17041 ttaattgagg caggtagttg aaggtgatct ctgtttggat tttcccatga tcgggaaaaa
    17101 gttcaccggc tattagtttg agcaaagtcg atttaccgac accattttct cctatgatcc
    17161 ctatacgatc ttgctgatag acacgaagag aatcaataga tagttctagc tggttgccaa
    17221 agctgacttg tagatttgtt atatttactg ctaaattttc catatgtttt acactcctat
    17281 ttcatgtaaa acaacaatta tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa
    17341 actgcatcac atcttttcct actctgaacg aaataactat gactaaatca ccaaatctgt
    17401 catagctttc acctattcta ctgtcgttcg aaaaaatgga catactccat ccatgaaatt
    17461 gttgtttata catgtctttt gaatttattt agttgttatt caaaagttcg aaacgtaatt
    17521 tcatcgatgc agtcattttt ccgacctcct tttttattag gttaccacac acgattcaaa
    17581 aaaagcaata gctactgaat agtaactgaa aaccttttat tcgaacatag tgatcttttc
    17641 tggtgctaaa cgaacagact cgtaaccttc ttctaccgct tttcctattg ataaaataat
    17701 aactggaaca taacggtctt tatccaaacc aaatgcctct gccaactgat ctgcttcgaa
    17761 tccaccaatt ggatttgttt catagccatg atcacgcgca actaacatga attgcatagc
    17821 cgctaagcta gaatcgactt tgactacatc gttcatttct tctcttgaaa aactcttgta
    17881 ataaggaatg atcgcagcta attgttgatc tctgacttct tttggcatct tccctgattc
    17941 gtaagcttgg ttgtagattt cttcgccata ttcgtaacat tgcatatcgc caaaaatcat
    18001 gaccattgca gaagaagtat cattttgtct cgtattgaaa cggatcaaag gacgcaagat
    18061 ttcttttgct tcctcgcttt cagcgaccac aaagcgccat ggtttcatat tgacagaaga
    18121 aggtgcagtc gtcgcttctt tgatcatttc cagcatttct tcgtgtggga ttttgtatgt
    18181 ttcatcatat acgcgaacgg atcttctacc agtcataatt gttgaaaaat catttttttg
    18241 ttccattatt attaacctct ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt
    18301 tatctaaaag tgttagaaat tgtttcattt ctacatcgct gaaagccggg gatagcacgg
    18361 aacctgtatc tttatggtcc atttcacaat gcttgatttt cttctgggct ttttctgtta
    18421 gcgctacata cacttcccgg ttgttctgag gatttctctg acgcgaaaca tagccatttg
    18481 cttccaatac cttcagatga cgagtaactg ctccttgatc aatctgcaac ttttcttgca
    18541 aactgctttg tagcaatgga ttttcttctt tcagtctcat catgatctcg tatctagtaa
    18601 gacttatacc gatattttct tcaaagatct ggacgatttt ttgatcggct aatttgattt
    18661 gatataaaag gtgactaatc tcttctaaag acataatttc ctcctttcta ataattgact
    18721 cgtcaacgat tgacttgtca ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg
    18781 tgtaataaaa aaaacgctct caatagaaaa gtatagagaa aagaaatttt ttgtagaatg
    18841 aacggaaaaa atgataaaga aagcacaacg ctaaaaccaa gattattgat tcttatcaaa
    18901 ctatagaaga tagctcttct gcagtagata attttacacc gccaaaaatt tccgaaatcg
    18961 ccttttgcaa gaatcccgtc gaatattaga aattggatca gtatgcttaa acagtaataa
    19021 aaaagttatg acaagatgtt gtcacaacct tttttgagta aacggtgttc aaaaacttgc
    19081 atctgcggta ataagttcaa ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt
    19141 cttactactt gatgcagggc agctttttca ctacctcttt ttaataacca taaaatccta
    19201 ttccttcata tctccagccc atcggtgtta gccaatctct ttccttagca ctcaaggtat
    19261 agtgatgggt accagttttt tcatttggat taaatagtcg ataaattggc ttggaaccac
    19321 cagaataaaa acttagacct tcataacgcc aaccgatttt tgttaaagag ttgatctcag
    19381 attgtgccat tgtgtaatga tggtctccac tgtaaggatt gtataaacgg tagacaggtt
    19441 gtccgctgtt tggtgcttgc caagcaatcc cttcatattc ccagcctaca gttggcagat
    19501 aatttttttc tccggcattc aatgtatata aatgttctcc tgtatgacgg ttatacagac
    19561 gatacatcgg agaagttgaa gttggtgctg tatcatactg tgtcagatta taggtttcga
    19621 ttacattgtt caatgtagaa ccataattag gcgcagtggc ataacgacct gttagccaag
    19681 ccgtagcatc tttgtaagag ttcgtattgc ttttccatgc accggaatag tagtaaacgc
    19741 ctggctgaaa agaagtcgtc ttcaataccg ttacgttatc ttgcaatgat tctttgtacg
    19801 aaggatattt tctaaaagcg gcattcaccg taatccattg cccattcaca tattccgaag
    19861 taggcatcgt aacagattgg ccattatagc ttcctttaat cccaaataga ttgtaattag
    19921 gggcagaagc caaagtactg ttcccccagc cactttcaac gatggcttga gcaatcatga
    19981 ctgatgcata taagtcattg gcacttgcca aactttgtgc agttgaggca atactatcaa
    20041 tgaacgcttg ttgagaatta gcagatgagc gagcataaaa agcatagcca gtatagtttt
    20101 cgaattcaga caaatctgct tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt
    20161 tcgtgatttc ttctgcattc tctggattgt tctgagcttc tgctgatgta ctgtcttcgg
    20221 tttgtgtttg ttctgttgtt tcagttgttg gttcttcggt tgaaattgta ttttctgaga
    20281 cagtagctga ctgcaaagta ctttctgtat cgttttcaat tcctgtttct gaagaactcg
    20341 atgtctgagt tgcgacagtt tctgtcgatt cttcagctag cactgttgtg ttggcaactg
    20401 ctagatttcc taaaagaata acgctaaata ataaattctt tgctttcatc ttttttctcc
    20461 tttgaattat gaaacttttc ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat
    20521 gtccatattt aaataacatt tgtttctttt taataaattt gctttttctg cataaaaaga
    20581 cgtaaatgaa gaatattctt ctccattcac gccttaaatt gatggtcgtt tgaataacta
    20641 tttaacaagt aattctctct ggatattatc cgttatggat tatacttcct gtgtcgtcgg
    20701 acggataatc atgttgctga cagacatccg gtctggcgtt tctatcgcaa atgctacagc
    20761 agaagctata tcttcagatg ttaatcccca ttctttttgc gcttcatgaa gttcaagagc
    20821 agcctttttg tcgctgatgg tttgatataa ttcagtttga acagctcctg gagagatgat
    20881 cgtagatttg atgttatttt gccgttgctc ttgacgtaat ccttccatga ttgcacggac
    20941 agcaaatttt gttccgcaat aaacggcgga atcaggataa acaacatgtc ccgccacaga
    21001 atcagttgaa atgatatgcc cagatttttg tttttccatg attggaagta cggcggaaat
    21061 tccatttaac acacccatga tgttgatatc aagcatattt tgccattcat cacggtgacc
    21121 ttcaactaat ggagcggttg gcatgattcc tgcattattg tacaatacat cgattcgacc
    21181 gaacttttcc atcgctaaat cgataactgc ttggacttgt gcaaaatctc ttacatctgc
    21241 tgtttgaata tatagctcag cttctggtaa agattctttt attgctttta ggcgatcttc
    21301 tcggcgtgca gctataacta gtttagcccc tttttctgct aatagcttcg ttgttgcttc
    21361 accgattccg cttgaagcgc ccatgatgac aatcactttt tctgataaat cattcattct
    21421 attctcctcc tgttttgaaa aatctcttgc ttatactcac tatctcttat ttcatcttac
    21481 tgttcaaata atccgttcga caggtttgac gattttggca gggattcctg caacgatggt
    21541 attgtctggg acatctttcg taacagttgc attcgctgca acaatggcat tctcgccaat
    21601 cgttacgcct ggcagaatag ttgctcctgc tccgatccaa gcatttttct tgatgtggat
    21661 tggctttact gacaagcctc ttcgttcttt tggatcggtt agatggttca ccgtgatgag
    21721 gttgacacga ggaccaatca atactgaatc ttctaaacag atgccaccta agtcaacgaa
    21781 catcacttga cgattgataa agacattttt ccctatacta atatgtttgc cgaaatctgt
    21841 ataaaaaggc agcagaacag tcaccgtatc atctactttg tcactaatga tattttctag
    21901 ccgtttgcgg atatctgctg gttcatggta cccagtattc agcgctgcta aaggctcttc
    21961 gttttccgct tggatctgat ggatcacttc aaagagcgga tccgttactc gaatctcttt
    22021 attgacgatt tgtttttcga tgaggcttgt cattttgctc cctcctttta tccttacttt
    22081 ataacggacg gcttatgatg tctaatactt ataaaacata cttaactatt ccttttaact
    22141 atacttagtg gtatgctttt ttaaaaacag gagggaaaaa atggatattc gtatactgac
    22201 gtatttttta gcagtagcta gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat
    22261 cacgcaacct actctctcaa aacaactgaa agatttagaa gaagaacttg gtgtgacact
    22321 tttcactcga ggctcccgag agatacagtt gacggattct ggtcgatatc tttattcaaa
    22381 aggaaaagaa atcttgtcgt tggtagaaaa aacggcgaac aatttaacaa atgacgcaat
    22441 catctctgga gaaatctaca ttggcggagg cgaaactaaa gcaatctctt tcatttctga
    22501 tagccttcat tcgttgatca aggcgcatcc tgatatccag tttcatttat atagcgggaa
    22561 tgcagatgat atcatagaca aactggatag cggattactt gattttggac tcgtaatcga
    22621 accgacaaac aaacaaaaat accagtcatt tcgactgcca gcccaagatc gttggggact
    22681 tttgatacat cggaatcatc ctttagcaaa gcaaacagtc atccatccgc aggaactgaa
    22741 aactgttcct ttgatcattt cccggcaaac gtcagtggac agtcaactag ctgaatggct
    22801 aggtttcagc atagatcagt tatcgatcgt cggaacatat aacttattgt acaatgcttc
    22861 tttgatggtc gataacggga cttttggtgc attagctcta gatgggatca tcaatactta
    22921 tgggacggaa aaagtattca tcccactcga tccgccatta caagcttcac tgaatctcat
    22981 ttggaaaaaa gaccagcctc tttctcctgc tgctcgtaaa tttttagatg tcctaacgga
    23041 gaaatacagt taagtagagt ttttttcctt cgctttcagc tttttaacaa cgaataagtt
    23101 aagcaaaaga tccaatgatt aaataacata agtttcttaa tttgttcctt ccttttattt
    23161 ctgataattc cagtaaatta ctataattat aaaaaaagaa ttgacaaaac ataactgtct
    23221 atataaaatt gattacgtta acaataattc gtttcatgtg actagatcat actaggcatg
    23281 aaaaatctct gaggaacatg gtttccctag gaggtttttc gtgtctattt ttgtttggcc
    23341 gcaatcaact ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa
    23401 atcaaaaaga tctttaatca aaatgcagta ttggtagatg acggtggaca ggaaaaagta
    23461 gctattggaa aagggattgg atttgacaga aaaagaaatg atttgatttt tgatcgtgat
    23521 gtcgaacgga tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat
    23581 cagattgatg aacgcttttt atttgcagca gaaacgatca ttgatcatgc ggaaactgtt
    23641 ttgatggaaa agttgaatga acatgtattg atcgctttga ccgaccacat tgctttttct
    23701 gcggagaaca ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg
    23761 ctttacagtg atgaattcag tattgctcaa tgggcagtag agtatctgac aaaagagctt
    23821 gggattccat atacttatga cgaagcagga tatatcgcaa tccatattca cagcggacga
    23881 agcggtcgga acaataacca tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc
    23941 catttagtgg aatcagaatt ggaaacagat atccattcag aaccattttc actgaattat
    24001 tcaagactgg tgaatcattt acgactgctt ctacaacgaa cccatgcgca acaatatgct
    24061 gttttagaca cggaaatcgt agaaatggtc aaacgaaaat acccagaaag ctataagatt
    24121 tctaaaaaaa tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa
    24181 ctgggttact tggccatcca tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt
    24241 aacaatcatg aggaggaaaa aaattgaaag catatctaca acgaatggga cgttcccttc
    24301 aacttccagt tgcggtctta cctgctgctg ctttactagt agggatcgga aactggtggg
    24361 catcttatag taacgacatt gtcgctcatt ttttacaagc tggcggtaac gcagttttag
    24421 gtcaattacc tttgctgttt gccgttgggt tagcactagg gatgtcagtg gacaaatcag
    24481 gtgctgctgc cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg
    24541 attcagtagc tactttattg aatatcaaac cagaatcagt tgatccatct tttgctcaga
    24601 tcagtaacgt atttattggt attattgccg gattgattgc ggcagctctt tataatcgtt
    24661 tccacgaagc aaaattaccg atggctttat ctttctttag cggcaagcgt ctagtgccaa
    24721 tcttggctgc ctttgtcatg ttgctgatca gtgcagtgct actgctcgta tggcctccgg
    24781 tttactctgc tttagtttct tttgctaaat tcattttgag tttaggctgg ggtggtgcag
    24841 gtctttatgg cttcttcaac cgtttactga tccctacagg attgcatcaa gcaatgaact
    24901 ccgtgttctg gtttgacgta gcaggaatca atgatattgg taaatttttg gcttctgaag
    24961 gaacaaaagg cgtaacagga atgtatcaag ctggattttt cccaatcatg atgttcgggc
    25021 ttccagcagg agcatatgct atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag
    25081 cttccttgat gttagcagca gcttttgctt cattctttac tggtgtaaca gaaccacttg
    25141 aattctcatt tatgttcgtt gcttggccat tatatgtctt gcatgcaatt ttcactggga
    25201 tttctttagc agtgagtgca ttcttccatt ggacagcagg atttgccttt agtgccggat
    25261 ttgtcgatta cttcctatct ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg
    25321 tccaaggatt agtaacagca gtgatttact actttggctt taactttgct atcaaaaaat
    25381 tccatctgat gactcctggc cgcgaggaag cagatctttc agatgacgat actgcaacga
    25441 ctaattcaga caataaatat gcggcacaag caagcaaaat ctacgctgca ttaggcggag
    25501 ccgacaatgt aacttcgatc gataactgta ctacacgcct tcgtttgcaa gtcaaagata
    25561 caggcctgat cgatcaaaat aaaatcaaag ccactggcgt acctggtatg aagatcatcg
    25621 acggaaaaaa tgcgcaagtc atcgtaggta cagaagtaca attcgttgca gatgagatgg
    25681 caaaactaca tggtggtgct gcaacacgac ctgcacaaac aaacaccgtg gttaaaacag
    25741 aaacatttgc cggtggaacg atcgaacaag atatttacgc tatcgcaaat gggaaactga
    25801 ttccaatcac tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt gacggctatg
    25861 ctgtattacc agaaaatgga gaaatcttct ctccaatcgc tggtacgatc acaaacattt
    25921 tcccaacgaa acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata
    25981 tgggcatcaa cactgttgac ttaaaaggcg aaccattcac tttatatgta aaagaaggtc
    26041 aaaaagtcgc aagaggtcaa ttgattgctt tagttgatct tgccgctatt caatctgcag
    26101 gcaaaaacac ggatatggtc gttgtattca caaatggcga caaagtccaa agcttagaaa
    26161 tcgaacccgc acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct
    26221 gagccatcgt cttccccaga cttttttatc gataaacaaa caggagctga gacaaaaatc
    26281 ttgtctcagc tcctttttcc gaataaaaac ggcgttctcc tgccaactcc gcggtaataa
    26341 gtccagccaa acgtaacata tgaagagcta ttttttattt gactcttctt attacttgat
    26401 gcagaacagc ttttttacaa cctctgataa acggtgttca aaaaccagtt tatcggtatg
    26461 aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tcatactcaa
    26521 aactaatcgg ctttttcaca acctctttat acattattcc tgatccaata tttcttttaa
    26581 tgctgcttca agagaagtta atggttttcc caatactttt tctaaatcat cagaggaaat
    26641 agctaactga tcctttttaa tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg
    26701 agggaatccg gcttctttta gattttcgac aaacgctgca tcacttgctt caataatttc
    26761 taacttttta cctgtcgcac gctctaatgc cttggctaat tcttcatagt cagttagatt
    26821 gccggataat tctaagattt ctggagagtc actttctact agtacttttg ctgcagcttc
    26881 tgcatattca cgtttcaata cccaacctgt ttttccattt cctgcagcat aaacgaattg
    26941 accagttttt aaagcctgcc caatgatagg taattcattc tctacatacc aattattccg
    27001 taaaaatgta tgggaaatac ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg
    27061 cgctaataca ctgatcgagt gatcggcatc tgcaaaactt gtataagcga tataagatac
    27121 cccagctgct tgtgctgcct tcactacatt cttatgttcg gcttgacgat ttcctggaac
    27181 gccagagaca aaaagcaatc gatctattcc tttcaacgct tctttcattg agtctaaatc
    27241 atcataatca ccaatacgta tattaaatcc agcttctttc agtgcttctc ctttttcttc
    27301 actacgtact agtacataga tttcattttg tggaaccatc ttttttaaga attccagcgc
    27361 ataaccacca aaacctccag ttgctcctgt tactaaataa tgcatatttg ccactcccta
    27421 ttctttttat ttgttaagat ttacagctgt acatttataa attacacctt tatcataaag
    27481 agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc
    27541 tgcaatttag tgatgcaatc catgtacttg cctatattga aatgtttcaa gatacccgat
    27601 cactttctag tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa
    27661 tcatgagtca gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa ccgcagcctt
    27721 cccttgccaa aagtccagat cagatttctt tactagatgt ctatcgcagc atcgaaggta
    27781 atacacagct tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata
    27841 tccaagaagc tttagaagaa agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg
    27901 ctaagatcac acttgatagt ctgatacata aaatagccgt tttggaaaaa gaaaaaagac
    27961 cagaaaacac aggcctcgta gagagatttt tataattctc tctactgtgc ctgcattttt
    28021 ctgatcggaa tcgtctacat aaattctttt attctccggg ggacaaaagc cttgtcatgc
    28081 ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg
    28141 tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg aaagctattt
    28201 cccatttttc ctctctaatg ctcaggtcaa agcgctactt tcacgacctc tatattatca
    28261 tggtttattg actaccggta tccagatttc tcccatatat ttcccgttgg taactcccat
    28321 ttcgactaca gcatttggac ccccaacata ggcgtaatct tctacttctc ttaaaacatg
    28381 cccaaaggcc gtattcgtca aatcatttct taattcttca ggcgattctg ctaaagcgat
    28441 tacaacaaga tacgttcctt ttgggaatgg gatgattcgg gtagtgtttg gaacatcctg
    28501 actcgtttga acaccgacat agtacatcat tttcccgtta tagggttcgt tgactgtaaa
    28561 gaaataatga ttttcagcag cttgtttcaa tgcatctatt gttccattac cgataacttt
    28621 ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa tccgtattgc tactcttcaa
    28681 ttccacacca atacccgtca ccgtaaaatc tggtttttct tcgatcctat actttgccat
    28741 ttctcccacc ctttctatta tattaagttc atcttacata aaattaaagg gaaatgacac
    28801 gattacgctc ttttttctag ggatattttt attttttaac tatttttttg ctttcactgc
    28861 aatccaaatc tcactgtaaa cattactgaa atcctctccc cattttgtaa aagagatctc
    28921 gggagcttcg actaactcat aatctgaaga aggaagccat tctgcataga ttttccccca
    28981 agtatcttgt aaagtttggg gaaatggccc tttattagga aaaatggccc aagtgtgttc
    29041 ttctatagca agcatttcta aatcagcgta cggattttct tttgtcgtag caaagccaat
    29101 catgtgcatc aaactgccct tttcttccaa gcgggctcca tcaaatccat aagaaacgtt
    29161 gattacttga tttggatata aatctcctaa cttatgcatc tcttcccttt gagtctccgt
    29221 gatcgtctgc gctaactttt gtatttcttg attgactcct tcaaatttga caggaacttt
    29281 tttcgccaca cctgccacat aaaactgttc tttcttttcg atacgaaatt ccatcgattt
    29341 tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct
    29401 gctgatttct gaaggcagat atcctgacca atcacgaaaa gctcttgaaa aaccttcgac
    29461 tgtttcatat ccatatctaa acgccgtttc tgtcactctt gatccatcta ggagatcgcg
    29521 attagccaaa gccaatctac ggtttttgat atagtccatt agcgacatcc ctgcaataaa
    29581 cgtaaagatg cgttttaatt gatattcaga gattcctgta ttttcggcta ttattttata
    29641 ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc aattggttca gatctttaag
    29701 catatttatc ttcctccttg cttgcctttt tatcatatta tcaaagaaag tcaattactt
    29761 gatgaatcag gaaccaattt agtcagatgt agaaaatgtt gtatggatct aaagaaaatt
    29821 ctcaagcagc gtttcgtaac ggataattgg tattatctgc ctatctatgt
//
LOCUS       DAZKNM010000014        29452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000014 DAZKNM010000000
VERSION     DAZKNM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.014"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..783
                     /locus_tag="ACG8M9_000543"
     CDS             127..783
                     /locus_tag="ACG8M9_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0377342.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1225..2022)
                     /locus_tag="ACG8M9_000544"
     CDS             complement(1225..2022)
                     /locus_tag="ACG8M9_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0377343.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2019..3203)
                     /locus_tag="ACG8M9_000545"
     CDS             complement(2019..3203)
                     /locus_tag="ACG8M9_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0377344.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3228..4475)
                     /locus_tag="ACG8M9_000546"
     CDS             complement(3228..4475)
                     /locus_tag="ACG8M9_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0377345.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4690..6435
                     /gene="dnaX"
                     /locus_tag="ACG8M9_000547"
     CDS             4690..6435
                     /gene="dnaX"
                     /locus_tag="ACG8M9_000547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0377346.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6455..6769
                     /locus_tag="ACG8M9_000548"
     CDS             6455..6769
                     /locus_tag="ACG8M9_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0377347.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6794..7390
                     /gene="recR"
                     /locus_tag="ACG8M9_000549"
     CDS             6794..7390
                     /gene="recR"
                     /locus_tag="ACG8M9_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0377348.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7454..8098
                     /gene="tmk"
                     /locus_tag="ACG8M9_000550"
     CDS             7454..8098
                     /gene="tmk"
                     /locus_tag="ACG8M9_000550"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0377349.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8113..8442
                     /locus_tag="ACG8M9_000551"
     CDS             8113..8442
                     /locus_tag="ACG8M9_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0377350.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8456..9394
                     /gene="holB"
                     /locus_tag="ACG8M9_000552"
     CDS             8456..9394
                     /gene="holB"
                     /locus_tag="ACG8M9_000552"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0377351.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9430..10254
                     /locus_tag="ACG8M9_000553"
     CDS             9430..10254
                     /locus_tag="ACG8M9_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0377352.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10247..10594
                     /locus_tag="ACG8M9_000554"
     CDS             10247..10594
                     /locus_tag="ACG8M9_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0377353.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10663..11535
                     /gene="rsmI"
                     /locus_tag="ACG8M9_000555"
     CDS             10663..11535
                     /gene="rsmI"
                     /locus_tag="ACG8M9_000555"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0377354.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11644..12765
                     /gene="dinB"
                     /locus_tag="ACG8M9_000556"
     CDS             11644..12765
                     /gene="dinB"
                     /locus_tag="ACG8M9_000556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0377355.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12819..13421)
                     /gene="nrdG"
                     /locus_tag="ACG8M9_000557"
     CDS             complement(12819..13421)
                     /gene="nrdG"
                     /locus_tag="ACG8M9_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0377356.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13736..15925)
                     /gene="nrdD"
                     /locus_tag="ACG8M9_000558"
     CDS             complement(13736..15925)
                     /gene="nrdD"
                     /locus_tag="ACG8M9_000558"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0377357.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16251..17018
                     /locus_tag="ACG8M9_000559"
     CDS             16251..17018
                     /locus_tag="ACG8M9_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377358.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            17008..19050
                     /locus_tag="ACG8M9_000560"
     CDS             17008..19050
                     /locus_tag="ACG8M9_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377359.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            19331..19480
                     /locus_tag="ACG8M9_000561"
     CDS             19331..19480
                     /locus_tag="ACG8M9_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0377360.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            19491..21005
                     /gene="dltA"
                     /locus_tag="ACG8M9_000562"
     CDS             19491..21005
                     /gene="dltA"
                     /locus_tag="ACG8M9_000562"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0377361.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            21002..22207
                     /gene="dltB"
                     /locus_tag="ACG8M9_000563"
     CDS             21002..22207
                     /gene="dltB"
                     /locus_tag="ACG8M9_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0377362.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            22252..22485
                     /gene="dltC"
                     /locus_tag="ACG8M9_000564"
     CDS             22252..22485
                     /gene="dltC"
                     /locus_tag="ACG8M9_000564"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0377363.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            22488..23756
                     /gene="dltD"
                     /locus_tag="ACG8M9_000565"
     CDS             22488..23756
                     /gene="dltD"
                     /locus_tag="ACG8M9_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0377364.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            23897..24979
                     /locus_tag="ACG8M9_000566"
     CDS             23897..24979
                     /locus_tag="ACG8M9_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0377365.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24979..25782
                     /locus_tag="ACG8M9_000567"
     CDS             24979..25782
                     /locus_tag="ACG8M9_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0377366.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            25852..26592
                     /locus_tag="ACG8M9_000568"
     CDS             25852..26592
                     /locus_tag="ACG8M9_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0377367.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            26603..27607
                     /locus_tag="ACG8M9_000569"
     CDS             26603..27607
                     /locus_tag="ACG8M9_000569"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0377368.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(27652..>29452)
                     /locus_tag="ACG8M9_000570"
     CDS             complement(27652..>29452)
                     /locus_tag="ACG8M9_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377369.1"
                     /translation="LRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVF
                     YTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEK
                     ILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKFYLGTTGPL
                     ISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAV
                     SEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIG
                     VLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAII
                     HCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKND
                     LLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEML
                     CSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRE
                     THAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMK
                     DVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9311 a         5184 c         6063 g         8894 t
ORIGIN      
        1 tcgcataagt gatggaaaaa ggtacttaac aaatgttaat atcattagta aaataccttt
       61 ggactttaaa aagttttaaa gtactgtatg atctttttag atgaaagttt ggatggggga
      121 gcatcattgg atagtagagt cttaaaggaa tatttggata aaaatgattt tccgattata
      181 cgcaaaggat atcataaata tttgacattc gaagggatgg aagattccca tacgtatatc
      241 ttgaaagagg gaacggtaaa agcaagtgtt atttcaaatg atgggagaga atttaacctt
      301 cgctatatca aagatcttga aatcgtctct cttttaaaag acgaatattc acaattcatc
      361 gattcgccgt ataatattcg agttgaatca gaatatgcag agttttatcg aatcaatcgt
      421 gtgcgctttt ggcaaaatat caaccaagac ccaaagctac aacactatgt aaaagagtat
      481 taccgctatc gtctgcttta ttcgatgaaa aaaatgcagc aaatgctatt aaacggaaag
      541 ctgggagctg tttgtacgca actttacgaa ttatatgatt tattcggtgt gcggacagaa
      601 gaagggatgc tgatcgattt ttcgatcacg aatgaagaaa tcgccaaatt ttgtggcatc
      661 tcaactgcta gcagtgtaaa cagaatttta aaacaactga aaaaagatgg tattatcacc
      721 gttaaagacc atcatatcgt tattttgaat atggacttgt tagaagacaa tattatcttt
      781 tgatgaacaa agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacgga
      841 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
      901 accgtttacc aagaggttgt gagcctgccg tttaactctg agtcacaagg aacaatttca
      961 aaaaacagct tttcatgttt gatgaatatt gtgacttgtg accgaggagt tggtaggaga
     1021 acaccgttta ccaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaagagccaa
     1081 gtaaaaacag tctcttatgt ttttaacttg gctggactta tcaccgtaga tgtaagcttt
     1141 tgaacgccgt ttatttgcaa caaaaggggc tgtgacacga tttttgtcac agcccttttt
     1201 ttgcgaacat tcatttagca atttttagaa acttctgccg ccaccgccaa aagtcccgcc
     1261 accgctagaa tgggtagtac ttccgccacc gccgcttcca ggaggtgggc ttttggggat
     1321 tcttcttgtc gtgacaaaag aatttgtcag acgatcggtt ttttcagtta atttgatact
     1381 ggatttttct cggaatggat atttgtagat gcctgatttc aattgataac gcgaaacagt
     1441 cacaaaataa aagacaagac taaggataag agccaaaaca acagcaatga tgatttctac
     1501 tgtcgtcaaa accttatagc gtgtaatttt gccagtttct tcgtctacac gataatgtcc
     1561 tcctggaact ccgtcttcca catatgttgc tgatttagac aggtaagctt ttgcagcatt
     1621 gtaataggca gcatttgtca tctgatcata aacatcatct aagatcgaat cgattctgct
     1681 atcagtcaaa taatcgatca tattaccaga agtcgaaatg tagatttcac gctgtcccat
     1741 atctaatagc aatactgagc cattttgatc atttccgact gcgtctctta agtaattatc
     1801 agcaaagtct cttggttctt ctgaattaga tgtagtcgtt acgatcaaga ctcgtccttt
     1861 gattttttca ttcagctttt ctgcctgttg ttccaaagat tggatttgat ctgttgtgaa
     1921 taacccagcc tcatcattta ccgtagctgt atctgcatga gtagccaccg gccagccaac
     1981 acttactagc aaaagaaaga ataaaaaaaa ttttttcatc atatcaagta acctccgatc
     2041 agaaagagga tcgctagccc agcgaaaatc ccaagagcgg tcagtcccaa ttttttctgg
     2101 ctgatcggca ggacaccgct gacctttcct gtttgtccat tcattgcgta gtagtagact
     2161 tttttacttg agtcattgct tcgataagta acaagccata ccggcagtaa tacgtaatta
     2221 tttacttcgt ttgtgattga agcagaagtt cgcacaccgg tcaatgttgt atatccattt
     2281 gcggtatctt ttaacagctt ctctgaatag tcttttaatt cagattgaat ctgtgatttg
     2341 attgcctcat attcgatatc tcttttttct gcttggaacc cggctaaata ctggctttta
     2401 aaatcaattg ctttatctaa cgggaaaggt tgcacgcctt cgaccatctt ttgttgcgta
     2461 ttttttgata aggcattttt tactagttct ttaaaagaaa gcttgcctgc tcttgaaaca
     2521 gcaaattgct tcgtttctgt gtattcgatg tcacctatcc gccatatgcg gataactgta
     2581 ccattcgcct gcatggcacc atctacttca gcatctgtag cccaataagg gaaatacaca
     2641 cctgtcaact tatcgatttg atccttattg aaaaaagctt tgggaacaaa ccattttttc
     2701 ttggtccaag cgagaaatct gttgattgct tcttctttct cgacagcaaa aggaagtact
     2761 ttatttggca agaattctcc gcttaaacgt cccgatagta cgacgggatt atggcagtag
     2821 taacaataag tcgcagcagt tgtcgcatca gtcacgatct ctgcaccaca gttgggacaa
     2881 agaaaaagat ctatcgctgc ggtattatct gcagattcgt ccgctgtatc tgaaaaaccg
     2941 cctgcctcgt caaccgcttt gcgctcggct tcattcatat cggcaagctg ttcttgggct
     3001 gtaaaagtaa aatcttcggt aggtgaaggt tcttctttgc tttcttcgcc agccatacgc
     3061 gcttcttttt gcttttgctc atattggctg acttcttctt ctgtatagat attcaggcag
     3121 tattcgcaat gaaatttttg gtctttggga tcaaaagtca acggaccgcc gcaattagga
     3181 catttatgtg taagaacatc catcagctgc acacctttct aaataaatta attaacgggg
     3241 attcctttaa acggtttacc acagtttgga cagaatttag ggatgccgtt ggaaagatcc
     3301 acgatctcat tgcattcact gcatcgcatt tgtagcttag gctgaacgtt ttctacaggt
     3361 ttggcagctc cgcagttgga acagaatttt ccggaatttt ctgtcccgca aactggacat
     3421 gtccaagtat ccgcatttcc ttgtgcagtt tgttgatttt gtttttctgc ttgttgtttg
     3481 atttgttctt ggttattttg agctgcttga gaaagataag aaccatttgc attcattccc
     3541 atacctacac ccataaagcc ggtcattgct cctgcttcat tttttccggc tgcttccata
     3601 ccacgtgcaa tggaaccttg cacgtagcct tcacgtacgc taggatcacc tagcatcgcc
     3661 ccttcgttac gcatattgat cagtttcaca gaatcatcgg tgtaagaaat gctggctaca
     3721 gccacagaaa caatctccat cccacgtaat tcacgccaag aatcatccaa tgcagtatcc
     3781 atgtatttgc ttaattccaa actcttagat ggtacataag aaatccgttg accatctgca
     3841 gacatctgat tgattgctga ttgtagcgct gttaggaatt cagctaaata ctgctcattg
     3901 atatcattga tttccacttg tgttttgttt ttagggattg cgtttgtata aaataaaatc
     3961 gggtcaacga catgaataga ataagtgcca tgtgcccgta agaataattc tgcgttatag
     4021 aaattgtcaa agtagttcaa cggcgcgctt gtaccgaatt tgattccctt tatttcttgg
     4081 agattgatat aaaaaacttg ttgtttctgc ggtgtgacgc cgccaaattt gaatcggtca
     4141 aaagtttcag caattgcttc tttcagtgtt ccattgaaca tggaaggagc tgcgtcattt
     4201 ttaatggtgt aataaccttc ctcagcggta taatcgatga tttttccacc atcgaccaaa
     4261 agcatcatca tgtttggata gacatggacg actgttccgt ctgttaaaac atcttctgtt
     4321 cctttacgat tggagccacg tttgtcatct ttgcggactt tgacaccttt tgtcatgacc
     4381 gttgtatcac tcatattgtc tggttcgatg acttctagcc attgatctgc cagaccgccg
     4441 ccaaccatac ttgttgctgc tttgataaga cccataaaaa attcctcctc tgattcagct
     4501 agtgccaaac aattattgtt tatttattat accataggaa gatgtgaaac ttctcggact
     4561 agagacggaa ttgaaatgga aaattgcgta ttgtttaaat acctgaaaaa gccgagaagg
     4621 atagtagttt ttttacctaa tatcacgtat aataagagat agtgaatttt ttaaaaaagg
     4681 gggatcctta tggcatatca agcactttat cgagtctggc gttcgcagcg tttcgaagac
     4741 atcgttggac aaaaagctgt gacacagaca ctaaaaaatg ctattgtttc ccacaaaacc
     4801 tcacatgcct acttgtttac aggacccaga ggaaccggaa agacgagtgc agcaaagatt
     4861 ttcgcaaaag caatcaactg tccgaacagt aaagacggtg agccatgcaa cgaatgcgaa
     4921 atgtgtcgat caattactgc tggtacgcaa gaagatgtga tcgaaatcga tgctgccagc
     4981 aataacggag tcgaagaaat ccgatttatc cgtgatcggg caaattatgc gcctacaaag
     5041 gcagcgtaca aagtgtacat tattgatgag gtacatatgc tttcaacagg agctttcaat
     5101 gccttgttga aaacattgga agagccaaaa gaaagtgtca tattcatctt agcaacgacc
     5161 gaacctcaca aaataccagc tacgatcatt tcacggactc agcgatttga ttttaaacgg
     5221 atcaatacgg cagatattgt cgaacatctt gaatttatct tgaaagaatc gactatcagc
     5281 tatgaaccag ctgcattgaa tgtgattgcc cgttctgcag aaggcgggat gcgggatgca
     5341 ttgagtattc ttgatcaggc aatctcgttt agtgaaggaa caattacttt agatgatgcc
     5401 atgcaagtga caggtagctt aactgacgaa atgatggaca gttacttgag tgcttgcgtg
     5461 aaaaaagaag taccagaagc attggaaaca ttgaaccaaa ttctcgcagc cggcaaagaa
     5521 tcacgtcgct ttctagagga tatgttacaa tactgccgtg acttgctgat gtatcagcaa
     5581 gcacctaacc tagttgagga acaatcgagt acgatcagaa gtacatttac agaattggca
     5641 caaactgttt cagcggatca gctgttttca atgataaaga ttttgagcga tactcaaaat
     5701 gaaattcgtt ttaccaatag tgccgctatt tatttggaag tcgctacagt caaacttgca
     5761 cagctaccgt ctgcttctgt tccttcagca gatgtagcat ttgcagatga tcagatagta
     5821 aaacagctac aacaagagat taaggaacta agaaacgaaa tcaaggaact acgcagtaat
     5881 acaggaattg agaatacaaa agtacagccg gtacagcaaa caaatcataa aaaacagaca
     5941 agtacctatc gtgtaccgac ggaacgagta tataaagttc tgcgggaagc gacaagacag
     6001 catctgaacc aagtaaaaga agtatgggaa gacttgctga tgagtctatc tgtcactcag
     6061 cgagcaatgc ttaaagcaag cgaaccagtt gcagctggac ctacaggact agtgattgcg
     6121 tttgattatg agattgtttg tcaaagagca gcaaatgacg aagaattgca gctttcggta
     6181 cacaacaact taagccgaat gatcaaagat tatgcgcctg atcctgttta tattacaaga
     6241 gaaagctggc cagaacttcg aagaaattat ttgacgcagg cacaagaaga gggaacagaa
     6301 aactcttttg cttctgaaga ctcagaagga gaaatcgagt tgcttcctca agaacaagaa
     6361 gtaccagaag aaacacttgt agtaaaagca gaggaactgt ttggctccga agctgttaca
     6421 gtggtagatg attaataaaa aggagtagat aattatgatg cgtggaatgg gcaatatgca
     6481 aggcatgatg aaacaagtac aaaaaatgca aaaagaaatg gcagaagcac aagagctgct
     6541 aaataaaaca gagtttacag gtaaagccac caatgaattg gtcaaagcgg tttttacagg
     6601 cgatcgtcgc atgaaagata tccaaatcaa tgaagaagtc gttgatcctg atgatatcga
     6661 gatgcttcag gatcttgtcg taatggcagt caatgatgcg atcgcacaaa ttgatgtgga
     6721 aactgagaaa aaattaggga aatacgcaaa aggtttgcca ggattttagt tttaaactga
     6781 aggagtaatg aatatgcaat atccagaacc aattgcgaaa ctgatcgaca gctacatgaa
     6841 acttccagga atcggtcaga aaacagcgac aagactcgcc ttttatacga tcgatatgaa
     6901 agatgaagta gtaaatgaat ttgctaaatc tttgttaagt gtaaaacgcg atttgcattt
     6961 ttgtagtatc tgcggaaaca tcacagaaga agatccctgc gaaatatgta gaaatccttc
     7021 acgtgatcga agcgttatcc tagtggtcga agagccgaaa gatgtaatgg caatggaaaa
     7081 aatgcgagaa tatcacggac tttatcatgt tctccatggt gtgttatcac cgatggaagg
     7141 gacaggtcca gaagacatca atatttcttc tctgttgcag cggctgcatg atgcagatgt
     7201 aaaagaagtg attatcgcaa caaatgcaac aacagaggga gaagcaacag ctatgtatct
     7261 ctcacgtctg atcaaacccg caggtatcaa agtgactcgt ctagcacatg gtctgtctgt
     7321 aggctctgat atcgagtatg cagatgaagt tactttattg aaagctgtcg aaggaagacg
     7381 tgagttataa atttctaatt aagtgttttt tcagatacaa tataaaagca gcacaagaga
     7441 tgggggcaac aatgtgaacg gactatttat tacaatcgag gggcctgatg gtgcaggaaa
     7501 aaccagcatc ttgaatgaac tttaccctct tttgaaagaa gtagcaaaaa cagaaatcat
     7561 tcaaacaaga gaaccaggag gcattccgat tgctgaagag atccgagcag tgatccttga
     7621 tccaaaaaat gatcggatgg atgaacgtac ggaagcactg ctttatgcag cagcaagaag
     7681 acaacatcta gtcgaaaagg ttctgcctgc tctggcaaaa gggaaaatcg ttttatgcga
     7741 tagatttgtc gacagttctc ttgcttatca aggtgcaggc cgacgcatcg gcgttaccga
     7801 gattgctcgg ttgaatgagt ttgctacaga aggtactacg ccggatttta ccctttatct
     7861 tgacgtagat tcagacaccg gattaagaag aatcaagaaa aacagacaaa atcaaatcga
     7921 ccgtttagat tcggaaggtt tggaattcca ccagcgtgta cgtcatgctt acttaaagct
     7981 ggcagaagag aatccggaaa gaatccacaa agttgatgca agaaaaagct ttgaagaagt
     8041 gctgcaaaca agttatcata caattattga acagtatccg caattttttg aaaactagga
     8101 aggtgaacgt ttatgaaaat tattttagcg attatccaag acaaagacag caaccgttta
     8161 tcaaatgaat tgattgatgc aaatatccgt gccacaaaac tatcatcgac aggcggcttc
     8221 cttaaggctg gaaacagtac gtttatcgtg ggtattgaag atgaccgtgt agaagaagca
     8281 ttggaaatca tcaaaaaaac gtgtgaatca agaaagcaat ttgtatctac acctgttaca
     8341 ctggatattt cgatggatgg gggcgtccct tatccagtag aagtagaagt aggcggtgcc
     8401 acagtcttcg tcttacctgt tgaaggattc catcaatttt aggaggagct gagctatgaa
     8461 tcaagaaata aacttggctc aaatgcaacc gatcgtgtat cagcagcttc aacggagttt
     8521 tgagcatggg cgtatcgctc atgcttatct ttttgaaggg gagaaaggaa caggaaaaca
     8581 tgaagtaggt atatggcttg cacagcacct gttctgtacc caaatgaaag acaatctgcc
     8641 ttgtggaaaa tgcaacaatt gtcagcggat cgaaaagaaa gaacatccag atgttttggt
     8701 tattgaaccg gaaggacaaa ccatcaaagt cgatcagatc agacgtttgc agacggaatt
     8761 cagtcgcagc ggttacgaat caagaaaaaa agtttttttg atcaaagaag ctgaaaaaat
     8821 gaatagcagt gcggcaaaca gtttgttgaa attcttggaa gagccccctg gagatttttt
     8881 agcggtgtta gaaacagatg cagtaggaag aattctccct actattcaat ctcgttgtca
     8941 agttctgcat tttcaagaac tttcgaaaga agcactgatc catcgattgc aagaagagca
     9001 gattccattg gaaaaagcaa aacttttggc tttcttaaca aacagtttag gaaaagcagt
     9061 tgaaatctct caagatgaat ggtttaatga tgctaaggac ttgatttatc aatggttcgt
     9121 ttacttgcaa aagcaagata ctcaagcctt tatctatgtc caaaaaaaat tggtaaaatc
     9181 atttaaagaa aaaacacaac agtttacagg attatctgtc ttgatgtttt attatcagga
     9241 agcacagcgt caagcagtag caaacggcga atatggcaaa gtgaaacaga taaaccagat
     9301 aattgaacgg atattgatcg cagagcagaa actacgcagc aatgtcagtt ttcaggctgt
     9361 tgcggaacag ttcgttttgc atacgatttt ttagcttgcc gcaaaggatg atgatcaagg
     9421 aggatggaaa tggtagaagt agtaggtgtc cgatatagag atgcaggtca tatctattat
     9481 tttgcaccag gagactctga atatgtttat agtgacaagg tattagtcga atctcagcaa
     9541 tccttacata ttgctactgt tgtccttcct aaaaaagaga ttgatcccaa tgaccttcct
     9601 gaagctatcc gtccgatctt gcataaagcg tcacaagctg aattagaaaa agacaaaaaa
     9661 aacaaaaaag atgcttatga tgcattgaaa atcgcaaaag aaaaagtgca gttgcgtgag
     9721 ttggaaatga aaatggtcaa agccgaatac acatttgatc gaaataaatt gattttttct
     9781 ttcacagcgg acgggcgaat tgactttaga gaattggtaa aagatctggc cgctgtattt
     9841 cgtactcgta ttgagcttcg acaaattggt gtccgcgatg aagcaaagat aataggcgga
     9901 attggtccat gtgggcgtcc attatgctgt tcttcttttt taggcgattt cgtgcctgta
     9961 tcaatcaaaa tggcaaaaga tcaaagttta tctttgaatc caacaaaaat ttccggttta
    10021 tgcggacgct tgatgtgctg tctgcaatat gagagcgaag aatacgagac agcgaaaaaa
    10081 gaactccctg actttggaaa agtaattgct acacctgatg gtaaagggaa agtcgtaggg
    10141 ttgaatctgc ttagccgtat cgttagtatt cggctgactg gacgggaaaa tcctgtcgaa
    10201 tatgaatggg atgaattgaa agaatttatc caagtaggtg agatgaatgg ataagagatc
    10261 attatacgac ggattgaatc aattagaatc agagttgaga aatacattaa cagaacttgt
    10321 tgaaatgaag catgctttgc atgagatcgt agaaaaaaat acaactttgg agatggaaaa
    10381 tcaacgtctg cgggaacacc tgcgggaatt gaaccaagtt gcacaagctc cagctgaaaa
    10441 tgtgaagcag gaactttcta agtctcgtat gaatcttgaa aaaatttatg aagaaggatt
    10501 ccatgtctgc tacgatttat atggatcaag aagggacaat gacgagcctt gtgcgttttg
    10561 tctagaagtg atctatcgcg aaagcggtcg atgaccaata acaagaggag actggagcgg
    10621 agaaatactg ctccagtctt tttattaagg agattgtgac agatgtataa tcaaaaaagt
    10681 ttcaagggaa atccggtata tggggattta tatcttgtac cgacgccaat tggaaattta
    10741 gaagatatga gctacagaag tgttcgtatg ctgcaagaat ccgatcttat cgcaagtgag
    10801 gatacgcgta atacacaaaa gttattgaat cattttgaaa tccatacacc ccaaaaaagt
    10861 ttacatgaac ataattataa agaacgtgtc cctcaattga ttgctttgtt aaaagaagga
    10921 aaaacgattg ctcaagtcag tgacgcagga atgccttcca tcagcgatcc tggacatgag
    10981 ctagttttag cctgcataaa agaagggatt tctgtgatct ctatcccagg acctacagct
    11041 gggatgacgg ctttgattgc ttcagggcta gtgcctcaac catttttatt ttatggcttc
    11101 cttccacgta agaaaaaaga acagaaagaa acattgaaaa agctaaaaga acagacagtt
    11161 acgctgattt tttatgagtc accttatcgg atcactgcta cattgacgaa tttcttagaa
    11221 atttttggaa atcgccaagt cgttttgtgt cgggaattga caaaggtcca tgaagaatac
    11281 cttagaggaa gtacggaaga gctactcgct tatatcgaag aacatccagt aaagggtgaa
    11341 tgttgtctgc ttgtagaagg gaatttgttc gaaatgccag tgacggaagt tgatgaagag
    11401 ttgggaacgt taaaggaaca agtagaagca aagatcgctt caggcgaaaa accaaatgct
    11461 gctatcaaag cagtagcgct gaaaaatggg ctgaaaaaac aagaagtcta ccgtcagtac
    11521 catgaattag actgagatag tataagttgt ctataatgaa agactaatga ggaaatcagc
    11581 tactaacttt tcaaatctct tgaacgcgaa cgaatgttct tgtataatga ataagaggtg
    11641 aggatgatgg agctgcaatt tcctttacat aatgatacat caagaaaaat cattcatatt
    11701 gatatggatg cttttttcgc ttcggttgaa gaacgagatc atcctgagtt tcgcggacat
    11761 ccattagtaa ttgccagaca ccccagtgac actggtgggc gtggagtagt gactacagca
    11821 aattatgaag caagaaaatt cggaattcat tcagcaatga gtgcacaaaa agcgtatgag
    11881 ctttgtccgc aagccatctt taagcctggc gaccatcata aatacagcga tatttccaaa
    11941 caagtacggg aagtattcta tcgttataca gatattgtgg aaccagttag tatcgatgaa
    12001 gcgtatcttg atgtgaccga aaataaaatc aattgcaagt cagccatcaa aatcgcccga
    12061 atgatccaag cagatatttg ggagagtgtc cagctaacat gttctgcggg agtaagttac
    12121 aataagtttt tggccaaatt agcatcagac tatcaaaaac ctagaggaat cacggtagtt
    12181 gcccctcaag acgctgtgcc atttttaaaa gctttgccaa ttgaaaaatt tcatggggta
    12241 gggaagaaaa ctgtacctag aatgcatgag ctagggattt atacaggaaa agacctatat
    12301 gaatgcacag aaatgatgtt gattcgaaat tttggaaaaa tgggctattc tctttatcgt
    12361 aaagtcagag gaatccatga ttctccagta aacgttacaa gagagcgaaa atctgtggga
    12421 aaagagcata cttatgggca gccgttgcaa acggaagaag cagtgctgac tcagttgcgc
    12481 caactagctg aaaaagtaga agaagcactg agaagagtcc aaaaacatgg gaaaacagta
    12541 gttttgaagg tcaggtacac ggactattct actgtgacca aaagagtgac attgccagaa
    12601 tatatctata aaaaagaagc actcttttat caagcaagcc ttatatggga ggaaatcttg
    12661 ggagtagaaa aaggaatccg attgttaggg attactttga cgaatctgga cccgatgact
    12721 tatgaaaaca tcgtccttcc attatgggaa aatcaagaaa tataaaaaca gctttgagag
    12781 ttgaaaccgg aaatccgttt tctctcccaa agctgttttt atatcaagtc ttgctttttg
    12841 atttgttcat aggtttctgt tgcatccgta catttttccc aaatcacaac ttgattggat
    12901 tctaaagatt tttggacgtc aatgatccgc tggttgctac ttccgcgaaa ttgtagtttt
    12961 aggtcacgtt tgcttaattc aaagcgtccg tcaacaagga tatcgatttg cgaaaggagt
    13021 tctaatttat cttctgaatc ttgtagcaat tcatcaaagg tatagcctgt ccatgaccag
    13081 atatcttttg tcgtaccgaa ctcttttcgg atacgtcgta cgacttgcag acaaacatct
    13141 gtattcaaaa aaggttcgcc accaagtaaa gttaatcctt gcacgtaatc gttgcgcaag
    13201 tcagcgatga tttgttcttc taattcaggt gtgaatggtt tgccatatcg aaaattttgt
    13261 gcagctttgt tgaagcagcc ttcgcaggca aataagcagc cactaacgta aagactgttg
    13321 cggacacctt caccatcgac aaaattaaac ggtttgtagt cagcaatata gttttggctg
    13381 tagtcttttg ctaaccattc ttttggtttt ggatttctca taaaaaaact tctttcattt
    13441 aaggttcaaa gatatagcta ttgaagataa aatctactcg accacataaa taagaacaaa
    13501 ctgacctaaa tccattgttc aagaaagctt gttcttattt tgatagaggg cgttcaaaga
    13561 tacaaagcca tcgaaagagg gtggtgaaaa aagacttgtc atagcctctt attctgaata
    13621 aacggtgttc aaaagcttgc atctgcagta ataagcccaa ctaaacgaaa aatatgagga
    13681 actatttttg tttagctatt cttattactt gatgcaggac agctttttca caacctcatt
    13741 tcatgtgttt cacacgtgag gaaatttctt tatggcggcc atgtaccatc ggacgagctt
    13801 gcggatttcc taaatagcca caagtacgtt tgactacatc acatgttttt ggatcatgat
    13861 ttccacattc tggacattcg aaccctcgtt ctgtcggttt gaaatcacct tcgaaaccac
    13921 attgatagca atgatcgatt ggcgtattcg taccaagata cccgattttg tcatatgcaa
    13981 aatcccatac agcttctaaa gctttcggat tttgttggag catagggtat tcacagtagt
    14041 ggatgaatcc acctgaacaa tattttggat aatccttctc aaattccaat ttttcaaatg
    14101 gcgttgggtt tttacgcaca tcatagtgga aactgtttgt ataatattct ttatctgtga
    14161 tattttcgat gatgccgaat ttttctgtat ccaaacggca gaaacggtca gttagacttt
    14221 cacttggagt tgaatagaca ctgaaatgat aaccatattc atccccccat gcatctgcat
    14281 tagcttttaa ttctttgacg atatcaagtg tgaaagcttt tgcttctggg tttgtttccc
    14341 attcgccacc gaagaaagct gaagcaactt catacaatcc aatgtagcct aacgatacag
    14401 tagctcgacg gtttttgaat aattcattga cagaatcggt acgtgaaaga cgtttcccga
    14461 aagcaccata catatataaa attggcgcat ttgctggaat ggcttcttta caacgttcta
    14521 cacgatagag aagcgcatct ttcatgattt ctaagcggtc agacagtaac tgccagaatt
    14581 tattctgatc tccttttgat tcgatcgcga ttcttggcaa attcaatgta acaacaccta
    14641 aattcattcg tccgacattt acttcttgac cattttcgtc tttccaccct tgcaagaatg
    14701 aacgacagcc cataggtact ttgaaacttc cagttaattc aacgatttta tcgtaattca
    14761 gaatatcagg atacattctt ttcgttgcac attccaatgc tagttgtttg atgtcgtaat
    14821 taggatcttc agggtttaag ttcacacctt ttttcaaagt gaagatcagt ttagggaaga
    14881 tagctgtccg cttttcactc cctaatccat tgattcgaat ctgtaaaatt gctttttgaa
    14941 tttctctttc aaaccagttt tctcctagtc caaatcctaa tgaagtgaaa ggagtttgtc
    15001 catttgaagt aaataaggta ttgatttcat attccaaact ttgcatagca tcgaagatat
    15061 cttttttggt ttttgctttt gcatattctt tttggcgttc tggcgaatcg atccattctt
    15121 ccgcgtcttt tagatgtttt ttatagttca attctgcaaa aggagcaagc aattcatctg
    15181 ttcggtcggc agaacagcca ccatattgac ttgacgctac attggcaata atctgtgaga
    15241 tttgtgctgt ggcggtctgg atagattttg gcgattcgac ttcggcattg ccgattttga
    15301 acccattgtt cagcatgcct ttgaaatcaa tcaagcagca attcgtcatc ggtgtataag
    15361 gatgataatc caaatcatgg taatggatat cgcctttttg atgcgcattt gctatatgtg
    15421 gtggcaacat tttcagtccg attgattttc caacgatccc ggctgtcaga tctcgctgtg
    15481 tattgaaaac attactgtct ttatttgcgt tttcgttcac gacagtttgg tccttgttga
    15541 ttagcttttc aatcgaaaag ttgatatctg ttgctttact gcgagcaaaa tctcgtttgg
    15601 tacgataatt gatgtactct tctgctaatt catattcgtt tttttctagt aatatatgtt
    15661 ccacgatgtt ttgaatctca tagattttga cattttcagc aaaccggctt ttgatttctt
    15721 ggacaaccaa gtctgcaatc atttgaattt tttcataagt aagtggtgat aatgagccgt
    15781 gaattttctt ttctgctttg atcagtgctt cattgatctt ttgttcatcg aaatcaacat
    15841 ggcggccatc gcgtttgatg actttcatct gggatgtctc tggctgctga gtaacgacag
    15901 ttttttcttc ataatttact ttcattatct tcaactcctg ctaaattgtt cttgtctatc
    15961 ataaaacaaa atcggggaag gagcaactaa atatcggcta tatatagtat tcaatgtgct
    16021 gtataattgg atttctatac tatatgtagt gtatatttta ttgtgaaaaa aggtgaaaac
    16081 ttgtagctgg tggatttgtt ttttgcatat ttaattttgc tgaataaaat aattgaataa
    16141 aattttttat tcagtccaag taaaagtgac aaaaacataa gagcaaatta ttcgtagata
    16201 tgctactatg caaataaata gaaatgaaaa atgaaagagg aggagaagaa ttggaaaaga
    16261 tgatcgaagt gaaacatcta agtaaaagct acggcagcaa actttctccc gtcaaagcct
    16321 tagataatat ctcattcaca gtagaagaag gcgagtttgt tgggattatg ggcccaagtg
    16381 gtgcaggaaa gacaacgctg atgaatatct tgtccacgat caatttgcct acgatgggca
    16441 gtgtctcgat ccaaggaaaa gaaatcacaa aaatgaaaaa cagtgagctc agtgactttc
    16501 gtcgaaaaaa actaggattc atttttcaag agttcaattt gatcgatacc ctaaatgcaa
    16561 aagacaatat cctattgccc ctagcagttg aacggatgac taaagaggaa atggaaaaga
    16621 gagtaagaca tgtagctcaa ttgttgaata tagaagaact gctcaaacgg tatcccgatg
    16681 aactttctgt cggacagaga caacgaattg cagctgctag agcacttgtt gttcagccac
    16741 aagtgatttt tgcagatgaa ccaactggct ctttggactc gaagtcggct acagaattgt
    16801 tgaactactt gaagaccatg aatcaaaaag aaaaagcaac gatactgtta gtcacccatg
    16861 atccatatac agcaagctac tgcgatagaa ttttatttat taaagatggt gtgatttttt
    16921 ctgaagttgt cagaagaggg acaagaagag agttctttga gaaagtaata gacatgcagg
    16981 cgacgattgg cggaggagga aaaatgaatg ctgtataaac tagccgttaa aagttttttt
    17041 cggcagtcac gaggctatct tgtttatttt ttcagtttga ctttatccgt aatgatctat
    17101 tattccttca gcgcaatgac ctacgatcaa tctttagtca gaagagcaag tcaagatact
    17161 tcgatagatg gtggattgag ttttggtgga tttatcatta ctgttgtttt gctttttttc
    17221 gtattcagtg ccaaccgatt tttcttgaat agaaggcaaa aggaaatagg catctatcag
    17281 ctttttggta tgaataaact gcagatatca ggtatatatg tgttagaaat catgataatt
    17341 ggattgttcg cctgtatttc tgggatatta ctcggtatta ttttttccaa actcttttca
    17401 atgattttgg ttcgaatgat ggatatggat cttactagtc cattttttat ctccattcca
    17461 tcagtagtag atactttatt tgcttttttc atcatccttt tagcagtatc tgtatattca
    17521 atatggaaaa tatggcgcta tccaatgatt cgaagttttg gagaaaaaga acaagcagat
    17581 agttcaacaa tgcgcttcag gacaaggcac cgattactcg cagtcatcgg gttactactt
    17641 ttaacgagcg gatattttgg agctagtcat tttagggaaa tcacttcatg gatgatcagt
    17701 tatcattatc ttgatacatt agttttcttt tttccttttt taattttttt cgtttgtgtg
    17761 gtcggtactt atttgttttt ctgttataca tttcggttat ttttgatcat attgagtaaa
    17821 tggaagattt attactatgg tatcaatttt ttgataatgg gtaacacgca agttcatttg
    17881 ctcaaaagtt ggcgtacaaa ttctctgatt acagtcattc tcggaatttc tttaatgaca
    17941 attggcggca cggctagtat ctctactatt ttatcctacc aggagaaagt ttcctccccg
    18001 atggactatc agtttgatgt tgagacagag aaaaaaatca agccgattct agcagaggaa
    18061 gggcagaaga taactaaaga aatcaccttg cattataaag tcattgggag tatctatagt
    18121 caaaatctcg aagaacctat gtttcaaact gctaatctga tcacggaaac acaatatcaa
    18181 gccttcagaa aagttaatcc agatttgccg aacatttctt tgaaatcacc tggtcatact
    18241 gttttgcttg atcaaatgca aactcttttt cggaatattt cattatatgg atctagtatt
    18301 ggactgccag ataatcaaga attgaagatt caacaaatgc ttccaagtgt attaggtgac
    18361 gaaacaatgg tatatctaag tcctgtactg attgtagatg ataccgtttt tgaaaaagca
    18421 aaaggtgtgg attatcaagt agtcaatgta gatgtatcag gtggaaataa tgaaaaaata
    18481 gatgaacgac tgagcaatga cctgtcgata aagtggctca atgctgttta ttattcttat
    18541 gatatcgtaa atggaagtct acaaggagaa attagcacaa agaaacttcc ggatgaccaa
    18601 atgaaggaag cacaatttag ccaagaaagc tctcgtttga atttctcttc cagctattcg
    18661 aaattgcgtg cagttaatcg gcgaatcggt atcaatattt ttgtcacact atttgtcggg
    18721 atgatcgtga ttattgctac tggtagtatt ttgacagtcc ggcaattagc agaagcagag
    18781 gaagaaaaag aaaattatca cctattgact aagctgggga ttccacaaag aaggatcaga
    18841 cggatgatct tcgagcaaaa cgcacttacc ttctttccac ccatgatatt agggatcact
    18901 cacgcagtat ttgcaatcaa tgtcttcact cagtatataa aaactgccga ttactggttg
    18961 gcttatcttg tgagtggctt attgatcctt atctatcttt tgctttatgt cattacgagt
    19021 cagttatatt gtcggattat cgaggaatga tatacggaat aagacaagat aataaataga
    19081 actaaatttt gaataccatt tatttggaat aagaggttgt gacacgattt ttgtcacaac
    19141 ctctttattt tttatatttt ttaaaagtac gagaaattat aaagaaaaaa gcagtgattt
    19201 gatgtgctat ttttataaaa gctatgtgac aaaactgtta gtaaaaaaac ggtttctgta
    19261 agctagacac atgtttcttt ttgtcaaaat tagttacact ttgtgtgagg ttatatgagg
    19321 agggaatcca atgaacaaat ggcgttctaa tccaaaattg aattattggg gaaaattcat
    19381 cattcgaaca attttttata gtgcagtact gctgttcctg atttatctgt atcactataa
    19441 aaatattcaa ggaggaactt ttatctataa cgaattttag gaggaagagt atggaaatca
    19501 aaacgattat tgaagcaatc gatgattggg gaataaatga acctgatcga gttgcttatc
    19561 aggcggatga gacgcatact tttggtgaat tgaaacaagc atcagacgca cttgcttatt
    19621 atttagaaaa aaaggtagaa ggcgatggac caattgtcgt ttttggcaat ttggagtttg
    19681 aaatgattgt ctcatttcta ggggttgtca aatctggcca tgcctatttg ccgattgaag
    19741 agcatacacc caaggagcgt atcctatcga tctttcgggt ggctaaacca tctatggtca
    19801 tcagtatcgg ggactggata gaagatattc cttcggttcc tgtcataaca aaaaaagaat
    19861 ttcaagaaat caagaagata ccagtaggat tttatcccag aaatagcgta aaaggtacag
    19921 aaaattttta tattattttt acttctggta ctactggaga accaaaaggc gtacaaatca
    19981 gtcatgataa tctagtcagc tttgttcaat ggacgatgga agactttggg atcaagcctg
    20041 gtatgcattt cttagcccaa gcgccatttt cttttgattt atcggtattt tctatttatc
    20101 ctgcattagt ttctggcgga atgctgaagc cgttaaataa agcagttgtt caagactttc
    20161 gtcaattatt cgcgacattg ccagccttga agttgaatgt gtgggtttct actccatcat
    20221 ttatggatat ttgcttgatg gaacctacat tcaatggaga aaatgtgccg gaattggaca
    20281 tgtttttatt ttgcggagaa gaattgacga aaaaaactgc agaaagtcta cttgatcgat
    20341 tcccaaatgc acggatatat aatacttacg gtccaacaga agcaactgta gctatttcaa
    20401 gtattcagat tgaccagcat gtactagata gttatgaccg actaccgatt ggctacgtaa
    20461 aaaaagacac gaaagtttct attgtccaag aaggtaaacc tgtttcaaaa ggagaaacgg
    20521 gtgaaatcat tattgcagga ccaagcgtat caaaaggtta cctgaacaat cccgaaaaga
    20581 cagctgctgc gttcttttgt caaaatgact tagcaagtta ccatacaggg gatgcaggta
    20641 gactagatga atatgggctt ttattttatg aaggacgtat ggatcagcag gtcaaattac
    20701 atggataccg gattgaactc ggggatatcg aacattatct gctccaagac aaccgaatca
    20761 aacaagctgt cgtcgtacca aaataccaag gaacaaaagt acagcagctg gttgcttttg
    20821 tcgtattaga aaacaaaacg aaacagccgg attttcagtt gactaaatct atcaaagaac
    20881 aattactaga agttgtaatg gattatatga ttcctcagaa gttccattat gtcgatctgc
    20941 tgcctcagac agttaatgga aaaatcgatc gaaaaaaatt aacagctgag gtgaatcctg
    21001 aatgatgtta cctcatatga tcccttatgc agagccgttt tatttttttc ttttagccat
    21061 tgcgcttgta ccgatcgttc tatctttatt gatatgggaa aaaagattac cagtttatca
    21121 atcagcagtg acacttttct ttttatttgt cagctttgga ggtaattatt ggcaacaggg
    21181 agcggcgctg attgggtatg ttttgtggca gacgatactt gtatggggct attttgttta
    21241 tcggcaaagg aaaaatcaga caggatggtt ttttgccgcc gttattttag cgataattcc
    21301 actgttttta acgaaagtga ctcctttttt tgaacatgga aaagcatcgt tacttggttt
    21361 tttaggaatc tcttatttaa cctttaaatc tgtacaaatg atcatggaaa taagagatgg
    21421 catgatcaaa acatataagc catttcgtta tatccagttt ctgctgttct ttccaacaat
    21481 ctcttctgga ccaatcgatc gctataggag atttgaaaaa gatcagctcc atccgcctgt
    21541 tcgtgataaa tatgttgatc ttttgggaaa aggcgtccaa aatatttttg tcggtttttt
    21601 atataaattc attatcggtt attattttgg tcaagtactg cttcctgtag tgggacgcat
    21661 tgccttacag catggtggta tttcatgggc attagtaggt tatatgtacg tgtacagtat
    21721 gtatttgttt tttgattttg ctggctatag cttatttgca gtcggtacaa gctacatgat
    21781 gggatatcag acaccaatca atttcaataa acctttttta tcatggaata tcaaagaatt
    21841 ctggaatcgt tggcatatga cgctgtcctt ttggttccgt gattatgtat atatgcgatt
    21901 gatgttcttc atgatgaaga aaaaattttt taaaagcaag atcgtaatgt caaatatcgg
    21961 ttattttgct ttgtttcttc taatgggtat atggcatgga ttgacgtggt actacatcgt
    22021 atatggactg tatcatgctt gtctgatctg tctgacagat gcatggatcc gatttaagaa
    22081 gaagcataaa gagcagttgc cttctaacaa atttacgcat ggcttagccg tatttttgac
    22141 atttcaagca gtatgtttta gcttcctgat tttttcagga tttttagata aacttttttt
    22201 tagctaacga atagtgacat attaatttat ataacaaagg agaaaaggaa aatggaagaa
    22261 caaattttat caatcttaga agaaataaca ggaacagatg aagtaagaaa aaatcgtgat
    22321 attgatctat ttgatgaagg gttgcttgat tctttagcat ctgttcaatt attagtcgag
    22381 ctagacggag agctggatat ccaagtccct gtatctgaat tcgagcgaga ggattggtgc
    22441 acaccgaata aaatcattca acaagcaact gcattgaagg gttgagtatg aaaaagaaac
    22501 tttttgctat tttcggcccg gttgttttag ctgttggctt actgtttgtg ttttttacct
    22561 cctcctatca agtagatatc tctaatccag aaacgattag acgtgcctct tcttccatgt
    22621 cagtgaacgt gttgaaagga gatgcgataa aaaattatgc attatctgaa aaacaatata
    22681 ttcctttttt tggctcaagt gaattaagca gaatcagtcc atttcatccg tctgttctgg
    22741 cagaaaaata ccagcgaaac tatcgtccat ttttgctggg agcacccggg acacaatcgt
    22801 taagccaata tatgatgatg cgttctgcag gagatgctat gaaaaacaag aaagtcgtct
    22861 ttattatttc tcctcagtgg ttcgttaaaa atggtgtaaa aactgattac tttaatacgt
    22921 attattctga acttcaaaca tacgactggt tgttttcaat gaagaaagta acgcctgctg
    22981 atcgctattt agctagaaga ttattaactt tctcaaaagt aaaagagaat gatacgttga
    23041 cggcgattct gcaaacgatc aaaaaaggga agctaccttt acctgaatct ttgaatcagc
    23101 tgcgcagcca atggaacatg ctgaagagag aggatgaagt attcagcaat attgggttga
    23161 aagatcgaca gcaaaaaatc gatcatgagt caaaacgatt accaaaacaa taccaagaaa
    23221 cagaattatc cattttagca aatcaaattg gagaacgaga aacgacgaat aatccattcg
    23281 ggttgaaaaa cgatttttat acgcatcgta tccgagccca cgagccagag ctcaaacagt
    23341 cacaaaaaaa ttgggattat cgtttttcgc cagaattttc tgattttcag ctggtcttgg
    23401 atcaattagc aaaaaaccat aacgaagtat tgttcatcat cccaccggtt aacgagaaat
    23461 ggagcgatta taccggacta tctcaagaaa tgttacaagg atttgccaaa aagatcaaat
    23521 ttcaattaaa tagccaagga ttcaatcgaa tagctgattt tgtcaatcaa gcgggtacca
    23581 actattttat ggaagacacg atccatctag gctggaaagg ctggttggct gcagatcaac
    23641 agatcagacc gtttttagaa gaaaatcata ttactgctag taagtatcat cttgacgatg
    23701 ctttttttag caaatcttgg caacatcaaa taccagataa actacaatta aaataaagaa
    23761 aacaggcgag tgttgctcgc ttgttttctt tatttttgaa tattctgaat aaaagataaa
    23821 aataccaagt ttgcctgaac aaaaatcata agcaatggta taatcacaat atgaatatag
    23881 aaatggggga aaacatttgg atacaaaaga agaacagcta cttattgatt taacttctgc
    23941 aaaaggagta cctggaaatg aagaagaggt aagagaagta ttcagagaat atgccaaacc
    24001 attcgctgat gatatttttt atgacggttt aggcagtgtg attgccaaac atggtgctgg
    24061 cggaggcccc aaggtcttta tttctggaca tatggacgaa gttggtttta tggtaacaaa
    24121 aataaccgaa aaaggattct tggaattcca aactcttgga ggttggtggg ggcaagtcat
    24181 gcttgctcag caagttgaaa tcaaaacacg tgaagggaaa atcgtccacg gtgtgattgg
    24241 ttccaaacca ccacatgttt taacacctca agtaagaaat aaaccttatg aaataaagga
    24301 tatgttcatt gatattggcg catcaagcca agaagaagcc aaagagtggg gcatacgtcc
    24361 tggagatatg gtcactccat atattgaata caaacgaatg aatggcagta aatatttact
    24421 ggcaaaagca tgggataatc gtatcgggac agctgtttct ctgcgagtat tagagaatct
    24481 atctaaagaa gcacacccaa atgttttatt cgcaggaagc gatgtgcaag aagaagtagg
    24541 cttgcgaggt gctcgaacaa gtacacacct tgtaaatccc gatattgctt ttgctttaga
    24601 tactggtaca gcaggagaca ctccaggaat gacaccaaaa gaagcagatt cgattttagg
    24661 aaaaggccca cagattttga tttttgatgc atctatgatc ccgcataaaa aattattgaa
    24721 ttttgttatt gatgtagcag aggaaatgga tattccattc caatatacag tgatttctgg
    24781 cggaggaact gatgcaggac aaatgcattt gacaagagat ggtgttccgt ctgtagccat
    24841 taccgttcct gttcgttatc tgcattccca tacatcagtg attcatgaag atgattattt
    24901 gaatacagtg aaactagtca cagaattagt gaaacggctt gatagtgaaa cagttgaaaa
    24961 aattcggagt tatgaataat gaataataga atacctattt ctgaatatcg gaaaaaagca
    25021 cgagtttctt tggaaaatca atggggaatc aatacttggc ttgttttctt atctataata
    25081 tttacgagcg tcatccagaa cttttttgac ggtttcagtc cttttccgga agaaagcatg
    25141 cagcagcact tattgagttt tctagtcgat aatttacttt tattcgcatt caactatgca
    25201 ttgttttaca ttgcgctagt agttgtacgg ggtggcagac caaaaagtaa ccttttattt
    25261 tcagttttcc aaagaaagta ttttggttcg atacttggca tcaatttgat caattcagtc
    25321 atcaactggc tactcaacag tttaatcttt ctgcccatgt ttttaatcag cggagtgagt
    25381 acttatatgc agctgctttt cagttcaaat gcggtaccta cgggtgtcat gccagaagat
    25441 tttcttgata tcagctttgt tttatcgatg atagggatga tttttgtttc agcactcatc
    25501 atgagtattg ttggcggttt attccaattt gctgcatgga caaagttcga ctatcctgat
    25561 ttgtcggtta ttcaatgttt gaaatatggc tggtttttat tgaaagatag aattggtacg
    25621 tatattttgt tgcagctgtc ttttatcggc tggtatttgc taggagcaat cgctttgttc
    25681 atcggtcttt tgtgggtcat tgtttatgtg aatgtgtcag ttgcagaatt ttatgaacaa
    25741 gcacggattg aaaaaggagg tccagacgcc tattttactt agcatgtaag cagtttattc
    25801 atcaaaaaaa taacttttgt ggtaggataa atctttgagg gggaatgtac gatgaaatta
    25861 actgttttag gatgcttagg agcgtatcct tataaaaatc aagggacaac aggttatttg
    25921 cttcaatctg acgggttcaa tttgttgatc gatgcaggta gtgctacgct gatcaaatta
    25981 caagaacacc ttgatccact tgatctagat gctgtgatcc ttagccatta tcatcatgat
    26041 catattgctg accttggtgt attacagtat tactggcaac ttcatcctaa gagaaatccg
    26101 gagacattgc tttcaattta tggacacaca aaagattcca ttcactttga agaattgacg
    26161 ttggaaaatg tctcaaaagg acatccttat tttgaagctg aagaattaaa aattggtcct
    26221 tttctgatta cttttatgga aacaatccat ccagtagtat gttacgccat gcgtatcgta
    26281 gaagaaacaa cggggaaagt attcgtattt acaggtgatt caggttattt ggaaagtttc
    26341 gctgattttg cgaaagatgc tgatttgttt ctagcagata cctatctttt tgaaggaaat
    26401 gaacatcatc atgcccattt cacttcaaaa gaagctggag aacttgcgaa atcagctaac
    26461 gtaaagaaac ttgtacttac acacctacct cagtttggtg atttagaaca attgaaaaat
    26521 gaagcgcaaa aggcagcagg caaagggatt gatatagaac ttgctgcagt tgacaaagtg
    26581 tttaacattt aggaggaaga acatgatttt tgtaccaaat gaaaaccacg acccaagaat
    26641 caatttagca attgaacaat atttgctgca agaaatgcct ttagatgaac ctattttgct
    26701 cttttatatc aatgaacctt caatcatcat cggcagaaac caaaatacgc ttgaagaaat
    26761 caatcgagat tatgtggaag aaaatggcat tcatgttgtc cgtcgcttaa gtggaggcgg
    26821 agctgtctac catgattttg gaaacctgaa ctttagtttt atcatgcctg atgatgggga
    26881 ttcattccgt gactttgcga aagtaacaca gccaatcatc caagcacttc atgaattggg
    26941 tgttgctggg gcggaattga aaggccgaaa tgatctagtc attgatggca tgaaattttc
    27001 tggcaacgcg atgtatgcta ccaacggtcg gatgtttgct catggaacaa ttatgttcga
    27061 ctctgatatc aatgaagtag tcaatgcatt aaaagtaaaa aaagacaaaa tcgaatcaaa
    27121 aggaatcaaa tcgatccgtt ctcgtgtaac gaatatcaaa ccatttcttc cagaagaaaa
    27181 gcaaaaaatg acaacagaag aatttcgtca agaaatcttg ttgaaaattt tcggcgtaga
    27241 gtcagtagaa gaagtaaaaa cgtacgaatt gactgatgaa gattggaaaa aaatcaatcg
    27301 aatctctgag gaatattatc gaaattggga ttggaattac gggaagtctc cagatttcaa
    27361 tttctctcgc caaaaacgtt tttctatcgg ttcgattgaa gtccatttga atgtctcaga
    27421 aggaatgatc aaagaagcaa aaatctttgg cgatttcttc ggactaggag atatctctga
    27481 cgtggaaaaa aaattggttg gacagaaata tgataaagca tctctatcaa aaatagtcag
    27541 ccaaattgat gtgaagaaat attttggtgc gatagaacca gaagaattgc ttaccttact
    27601 ttattgaaaa taaaaaatag cggaatccac atatatggat tccgctattt tttatacagg
    27661 attcgggtaa tttcgagaga gttgaacagt gatccgatca atgaaagtat ctttttcctg
    27721 cgttagtgtt cctgtgtaac gatccgctac gtctttcatc atcattaagt caaaacgttc
    27781 ctgatcatct aattcttctg tgatgcctcg actaaatagg tttgtatcgt aatgtgcact
    27841 atgttcgcaa caaatgacga aatgttcctc ttgttcgtgt atcgacaaat aaatttttcg
    27901 atacagtgga ttttcgatgg catgagtttc tctaaaggaa tgctctaaag cgtggatcaa
    27961 aagttgagcc agatcgtcat cgttgatagg aagttcttcg ggaagatctg cttggaaggt
    28021 tatctgaata ttccgtttgg cagctaactt ctggtaacgc gctaagataa gatttgtcac
    28081 ctggtggttg caaaaagaac acagcatttc gaaatttttt gcttcttcta atatcttttc
    28141 cacatagttt ttagcttttg tttggtccgt atgcattaaa tcttgcagat tctccatctc
    28201 ttggacaaat tccaggcgaa gtgtatttac ttcttggata tgctgtcgaa gctgctcata
    28261 atgttgctcc aacaaatcat ttttgatttt taagaaatct acttgtctcc gatctttgtc
    28321 ggtatttaag atgtgatcga ttagattgta gctgataata acgattagaa tgataatata
    28381 taaaatcgta gaccaattga tcgttgtttg atagatacct aatgccgttt ggatgtaaat
    28441 caagcaatgg ataatggcta ccaagacgat ccaaggtgta caagctacat aaaaacggtt
    28501 attcttatat gcttcgccaa tgcatgcaag tgaagtgact aaagtgctta taacactagc
    28561 aatattagca atcggcatta attcaggaag ttctgcagga gtaaaagttg cataaagcag
    28621 aacgccgata aaaatcaaaa gttggaacgt acaaaaaatt ccgtaccatt ttttatagta
    28681 aaacattcgg ctccaatagt agcagatgat gaaagctgac gttaagataa taaataaatg
    28741 agataaaaaa gaatgaacga atggttcaaa aagaatacca cttaaaatat cttctgatac
    28801 agcttcaaat cctaatgcca aagcaaagca acttaagaga ataagtgagt attcaagacg
    28861 tttttgttta tacatcaagt agatcgtata gaaaaaggtc cctatagcga taaaaacagc
    28921 gattatcatc gtcaatactt gcggcactga ttgtgaataa ataaaactaa tcaatggtcc
    28981 agttgttcct agataaaatt tcggtggcag tccagcatag ttctgataag gagatgagac
    29041 tgtaagggtc aatttttttc cgatataatt tgctggtaaa tctatcacag ccaatgagag
    29101 tccgggattg ctttcttcct gataatcttt ccgttggtat aaaatctttt cgcctagata
    29161 tacagtaatc cattgatggt ttcctttgat tagtaaattt gcttcgtcca acggtttttc
    29221 aagtgtcgtt tccataatga atgtttcgtc tttatccacg gtaggaatcg tacgagtgta
    29281 cttgctgtca aacttaaaat caggatcatc ttttgtatag aaaacccacg aatcttttat
    29341 ttctttcatt tgattgctgt ccgcaggaga aaaaataaaa taaaagaaat aagcaattcc
    29401 tgttaaaaat aacaatgata atataatgat tatgctccac tttatacgta aa
//
LOCUS       DAZKNM010000015        27254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000015 DAZKNM010000000
VERSION     DAZKNM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.015"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..1430
                     /locus_tag="ACG8M9_000571"
     CDS             261..1430
                     /locus_tag="ACG8M9_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0377370.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1903..2457
                     /locus_tag="ACG8M9_000572"
     CDS             1903..2457
                     /locus_tag="ACG8M9_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0377371.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2630..3067)
                     /locus_tag="ACG8M9_000573"
     CDS             complement(2630..3067)
                     /locus_tag="ACG8M9_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0377372.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3115..4062
                     /locus_tag="ACG8M9_000574"
                     /pseudo
     CDS             3115..4062
                     /locus_tag="ACG8M9_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4333..4768
                     /locus_tag="ACG8M9_000575"
                     /pseudo
     CDS             4333..4768
                     /locus_tag="ACG8M9_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4784..5617
                     /locus_tag="ACG8M9_000576"
     CDS             4784..5617
                     /locus_tag="ACG8M9_000576"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0377373.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5862..6887
                     /locus_tag="ACG8M9_000577"
     CDS             5862..6887
                     /locus_tag="ACG8M9_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0377374.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7025..7150
                     /locus_tag="ACG8M9_000578"
     CDS             7025..7150
                     /locus_tag="ACG8M9_000578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377375.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7175..8290
                     /locus_tag="ACG8M9_000579"
                     /pseudo
     CDS             7175..8290
                     /locus_tag="ACG8M9_000579"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8373..9269
                     /locus_tag="ACG8M9_000580"
                     /pseudo
     CDS             8373..9269
                     /locus_tag="ACG8M9_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9285..10109
                     /locus_tag="ACG8M9_000581"
     CDS             9285..10109
                     /locus_tag="ACG8M9_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377376.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10141..11451
                     /locus_tag="ACG8M9_000582"
     CDS             10141..11451
                     /locus_tag="ACG8M9_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0377377.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11599..12858
                     /locus_tag="ACG8M9_000583"
     CDS             11599..12858
                     /locus_tag="ACG8M9_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0377378.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12868..13740
                     /locus_tag="ACG8M9_000584"
     CDS             12868..13740
                     /locus_tag="ACG8M9_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377379.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13752..14582
                     /locus_tag="ACG8M9_000585"
     CDS             13752..14582
                     /locus_tag="ACG8M9_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377380.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14622..16805
                     /locus_tag="ACG8M9_000586"
     CDS             14622..16805
                     /locus_tag="ACG8M9_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0377381.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16866..18086
                     /locus_tag="ACG8M9_000587"
     CDS             16866..18086
                     /locus_tag="ACG8M9_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0377382.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18083..19543
                     /gene="gtfA"
                     /locus_tag="ACG8M9_000588"
     CDS             18083..19543
                     /gene="gtfA"
                     /locus_tag="ACG8M9_000588"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0377383.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19620..20726
                     /locus_tag="ACG8M9_000589"
                     /pseudo
     CDS             19620..20726
                     /locus_tag="ACG8M9_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21048..21257
                     /locus_tag="ACG8M9_000590"
     CDS             21048..21257
                     /locus_tag="ACG8M9_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0377384.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21250..21399
                     /locus_tag="ACG8M9_000591"
     CDS             21250..21399
                     /locus_tag="ACG8M9_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0377385.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21553..22218
                     /locus_tag="ACG8M9_000592"
     CDS             21553..22218
                     /locus_tag="ACG8M9_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0377386.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22502..23587)
                     /gene="xerS"
                     /locus_tag="ACG8M9_000593"
     CDS             complement(22502..23587)
                     /gene="xerS"
                     /locus_tag="ACG8M9_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0377387.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23921..24154
                     /locus_tag="ACG8M9_000594"
     CDS             23921..24154
                     /locus_tag="ACG8M9_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0377388.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24151..24558
                     /locus_tag="ACG8M9_000595"
     CDS             24151..24558
                     /locus_tag="ACG8M9_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0377389.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25695..26213)
                     /locus_tag="ACG8M9_000596"
     CDS             complement(25695..26213)
                     /locus_tag="ACG8M9_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0377390.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26580..26828
                     /locus_tag="ACG8M9_000597"
     CDS             26580..26828
                     /locus_tag="ACG8M9_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377391.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9322 a         4140 c         5033 g         8759 t
ORIGIN      
        1 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaagaaagtt
       61 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
      121 gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag
      181 atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa
      241 ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca
      301 ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat
      361 tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt
      421 caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa
      481 atttggtaat tcctagagat agaaatggta agttttctca acaaacttta cctgcttata
      541 aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta
      601 tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca
      661 tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag
      721 aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg
      781 tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc
      841 ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc
      901 taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta
      961 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
     1021 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
     1081 aattagttta tcaagcaagt tcaaaagaag aagcaaataa tcaaataagg tttatgatag
     1141 ataaatggaa aaagcagtat ccacgagtgg ttaaattgct catgaatcct gctatattaa
     1201 ccttttataa tttccctccg tcaatcagaa gaactattta ttcaactaat ttgattgaag
     1261 gttttaacaa acaattaaag aaatatacca aaagaaaaga gcaatttcct aatgaagaat
     1321 ctctggagag atttctagta tctcaattca acaactataa tcaaaagttt ctatgccgag
     1381 ttcataaagg ttttaaagaa atacatgata cattagaatc aatgttttaa cttgatcaac
     1441 agaatgggct tttccattta cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc
     1501 gtaaagttat aaaatattaa taaaaatatt ttattaatga aaagcttggt agcacggtta
     1561 ttcatatgct aatttatttc actttatagt aaggggagtt cttagtatat tgttcaaact
     1621 taaatttcat ataatttaat tatgtaaatt agctattcta agaacttcta cggataactg
     1681 cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta atttttttgt
     1741 acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa ctaaatccct
     1801 tgattttatt gactttcaaa agtttatata tataagttaa tgattatggt aaaatatata
     1861 cataatcatt aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa
     1921 ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat
     1981 ccggaaagag atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat
     2041 attataggat taaaagttgg agatatacga ggcaaaagta agcctattat tgttgaacat
     2101 aaaacaggta agcgaaaacc gatttttata gataatttga gagaagaaat tttattatat
     2161 actgaaggta aagaagaaaa tgattggtta tttccgagtc ggcaacaagg tcgacatata
     2221 acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg acgagatgat
     2281 ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa aactagagac
     2341 attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt
     2401 ggtataactg aagatgaaat aggagcatca cttcgtggat ttaaactagg tgtataattt
     2461 aaaaattttt ataaacagta aagacaaaga ttaaaagcta tttttatcta gaacgatttt
     2521 atttcataaa gttcgcggaa taaaagctgg ggttactggt acttggtata ggttcgtgtt
     2581 atataaacta agtaattaaa taacattttt aggttggaaa aggatgaaat taaaaaaaga
     2641 gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact gataactcaa
     2701 cgtttagcag actatctaat agggatagtc tgcttttttc gtttttgacc atacttttga
     2761 ccttctgcaa acttccttgc ttttcctctg tagaactcat gaaatctgca aaacgaattg
     2821 ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat atcatccata gtggttttaa
     2881 tatctgtatg tccaagtcga acatacactt ctttgacaga cgcaccagat tcaaagagca
     2941 gactacaatg ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa
     3001 tgatgatttt caaattgtga tttacatagt ctaaataaag gggcttattt tcttctgatg
     3061 aaaacaaaag ttgttcacca tcttttatag gtttgattct catttttaga tattatgccc
     3121 gcacgtattc atcacatttc tattgtgaat cgctttattc gaccgacatt tgacttttat
     3181 cataatatac tcggtttaaa attactaatg aaaacaatta atcaagacga tcataccatg
     3241 tatcacctgt ttttttcaga taatcaccac cgtgtgggaa ctgaactgac tttttttgaa
     3301 gtacaagaag ggaacaacca gttttttgga accaatacga ttgaacgaac gatactaaaa
     3361 gttccttccg aagcatcatt gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc
     3421 cattatggaa tcgaatcatt ttctggacga cctatcttac gttttgaagg gccagatgca
     3481 acacgattag ctttagttcc cttacgcgaa tttgaagata ttgatgatta tttcccgtat
     3541 gtcacagacc agatccctac agagcatgcc attttaggaa ttgatgccat tcaactacgc
     3601 gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg taggctggta gcacaaagaa
     3661 tacgtcagtt tcttttcggg tcatcaacga gtgacaatct tagaaaatca agacactcag
     3721 ttctatcaag aagttcagat tattcaagat cgtgagaatc cagttgccat tgaagggatt
     3781 ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat ctgatttaca acgcatcgac
     3841 aaacaattac aagagcgcaa ttttataaat tcaggaataa aagatcgtga attttttgta
     3901 tcactttatt atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac
     3961 ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg aagaaattcc tttattttta
     4021 ccgcagtact taaatttcat tcgtgaacaa gtggagcaat aattagctaa tcaaatccat
     4081 gacgaagagt aagaggttga ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat
     4141 tctaaagaat gttgatttga tgcggttcct atttttataa actatcattt tcgactaata
     4201 aaactactta tgagtcagcc tctctttttt tcttgatttt tatacctctt tctaggtaaa
     4261 ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa
     4321 ggggagtacc aaatgaacga gtatattgaa attatccacg caagagaaac aacttaaaaa
     4381 tgtcagtctc aaaattccca aaaataaaat tagtattttt accagcgtat ctggttcagg
     4441 aaaatcatcg attgtttttg atactattac ccaagaagct ggtcgtcaac tgaatgacac
     4501 ctatgatagt tttacccgtt tatttttacc taaatacagt cgtccagagg ttgatgagat
     4561 tgataatctt tctacggcga ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc
     4621 aacgttaggg acgattacta acatcaatgc gttatttcgt gtgttgtttt catgttttgc
     4681 aacacctagt ttaggatatg caaatgctta ttcatttaat gatcctagtg gaatgtgttc
     4741 tgtctgtcag ggaatcggta agacgattgc atttttaata ggattgcttt atttttcggc
     4801 tattgataat gctgattttg tattttctga tccatttacc gcagaagttt tgtctggaat
     4861 tgaggaacag gcaaagaatc atggttattt tgtattggtg cataatgtta attcagcaga
     4921 agatgttaaa actattcaga gaaattggcg ttttgagggg ttcattgttg ttggtgtacg
     4981 tgtaaaagaa tttttggaat taaataacgt ttttgatggt cctgttgttt atattgatac
     5041 gcatctttca aaagagacat taagagaact tgaacaacaa atgaatcgtt tatttgtaaa
     5101 tacaaatgat taccaagcta gtacattggc agtggaacat ttgattcatt atggtcatga
     5161 gaaaatagcg tttctttctt ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg
     5221 acatcaagct tatatcgata cattaaagaa atatcattta acctttaaat cgcattggat
     5281 atataacaac tctgaatttg agcgaattta tcaagaatta gctgaattta ctgcaattgt
     5341 tgttactgga gattatttag cggctaaatt agtaaaattt ttaaaaaata agaataaacg
     5401 agtgatggaa caattatcga ttattggatt tgatgatatt acttttgcag aattaatgga
     5461 tcctgcgttg acaacaatac gactaaatcc aggtaacaag ggggcgattg cttttcacca
     5521 actgattgga ctggccaaac aagaagaaat aaaaaatcaa gtactactat tagagggaga
     5581 attgatttct cgagactctg ttcacaaaat caaataaaaa agctctgtga aataagtata
     5641 gttaacaata aaatatccga acgatagaga gaatgaactg tgatctagca gttcctcgtc
     5701 gcaaattcac tagaattagc attatttact ctaaattgtt cggatatttt ttcttgtttt
     5761 taattatgat tacagtgcct atttttaacg aaatttgctt attttcaaaa tgtatgatta
     5821 tatgttagta aacactttca aatatgaaaa gaggagctat tatggtagga attagggatg
     5881 ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat gaggatccga
     5941 cactttcagt aaccgatgaa accaaacgaa agattgcaga atcggttaat ttttatcatt
     6001 atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat tgcattaata acgacagttt
     6061 ctgaagtaga tgagttggaa gatccctatt ttcgaacgat tcgcagaggg attcaagtcg
     6121 aagcagaaca aagcaaagtc aatttgaaga aaattattcg tttgtcagaa acaccgttgg
     6181 agcaagacga tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg
     6241 tgattgaaga agtatcagca attaattcga atgtcgtagt gattgatgac cctagtattg
     6301 aagctgaagt agatgcagtt tatactgatt taacaaaagc aacgaatcag catttagatc
     6361 gattgtataa aaaaggacac cgaaacattg cgtttatcgg aggaggacgt gttcgtttag
     6421 atagccaagg aaataaacac gtgtcagatg atgattatcg aaaaatagcc tatgagacgt
     6481 ggatgaaaca aaaaggatta gaaaaatata gtcaaattta tttagaaggc tggaccacac
     6541 ttgacggaat gcaggcagca gatttacttt tgaaaaaaca tgctaaagat aagttgccga
     6601 ctgctatcat ggtaggaaat gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa
     6661 atggtctaca tattccagaa gatatttcga ttgtgagttt tgataatatt gaagttgctg
     6721 aatttttaac acctgcatta aatacggtga atgtgaatac agaagaaatt ggaaaactag
     6781 cagttcgcat ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc aaagtgattg
     6841 tttctaataa tattattgtt agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc
     6901 caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc tcgacaaaac
     6961 attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa caggaggaat
     7021 taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa ctgggacatt
     7081 actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa ctttaagcta
     7141 tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg aatttgaaaa
     7201 acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat attgggttaa
     7261 attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc actctagcga
     7321 atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta tcaaagaaaa
     7381 tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc ttgaagacga
     7441 actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca aagaattatt
     7501 tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac tcagacaagc
     7561 tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac atattggaac
     7621 agagactggc tactggccat ttatttatca aaatggggga gaagtatttg ccgatgacaa
     7681 tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt atcgagatat
     7741 tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg ataagttaag
     7801 tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg caccgggatt
     7861 tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt
     7921 taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa aatacccaga
     7981 agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa tccaagcgga
     8041 aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg cttcttcaga
     8101 tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc gtccatacaa
     8161 taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta ttctgtctgg
     8221 tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg cgatattgag
     8281 cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt tcgtctgtat
     8341 attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt ggaaaatatc
     8401 gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta ggattaattt
     8461 tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa acggtagggt
     8521 tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca gacggtgaag
     8581 tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct agtattgttg
     8641 tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg tcgatttatc
     8701 ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg atttggcgct
     8761 ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata ggtctcgatg
     8821 gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt gtaggagttt
     8881 ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa attcctaaag
     8941 attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt ttcaacatta
     9001 cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt atcaatgctt
     9061 tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc atgtttaaat
     9121 cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac aagggatatg
     9181 gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca attcaaatga
     9241 agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa aataaatttt
     9301 tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg gcattacctt
     9361 ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa attccaccta
     9421 caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat ttattaccat
     9481 tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt ggctcggttt
     9541 tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc
     9601 ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt actccacagt
     9661 atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata ttaccagggg
     9721 tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta cctgatgaat
     9781 tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc aaaattatgt
     9841 tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg tttgcattta
     9901 aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct ttagctccgg
     9961 gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg gcaggttcgt
    10021 tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag
    10081 gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga gagtaagtac
    10141 atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa cgtattaggc
    10201 gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga tattgatgaa
    10261 aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc gaatgaaaat
    10321 cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc tgattttgtt
    10381 atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt tgatattcct
    10441 aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg gatttttaga
    10501 accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga agtttgtcca
    10561 aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg tgttttaaaa
    10621 gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt accagaattg
    10681 tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg gaaaattgca
    10741 ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga tttctatcca
    10801 acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt acgttttgaa
    10861 ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc agaataccat
    10921 ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat tccgttggat
    10981 gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg agaggacatt
    11041 gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata tatcatggat
    11101 tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc
    11161 acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg
    11221 caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg gacgaatatc
    11281 aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa aatttatcaa
    11341 gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat caaaaattta
    11401 gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta aaaagaagct
    11461 gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag taaaaatatt
    11521 gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc attcaaattg
    11581 tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg tatagcacta
    11641 ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta
    11701 gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga gattgctaaa
    11761 gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc gaatgctggt
    11821 gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa tctttatcct
    11881 caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt aaccgaagaa
    11941 ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga ggatcgtgta
    12001 tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac tgcttttgag
    12061 gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt agctgagatt
    12121 aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac tttaaatggt
    12181 tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa caatgtttgg
    12241 cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa agattttaat
    12301 cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg tgccggttat
    12361 aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg
    12421 gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt tccttttcca
    12481 gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt atcagtttct
    12541 gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac aacacctgaa
    12601 gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag
    12661 aatactcagg attcaccatt aagtggttta actgaacttg cttttactga tcgtcattta
    12721 gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac aacaaattat
    12781 ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt taatccaatg
    12841 aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa tcgttactgg
    12901 gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta ctttccaatg
    12961 gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa ttttgatttt
    13021 gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa atcgattggc
    13081 ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg tattttgatt
    13141 gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg gttctttttt
    13201 ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt taactatggt
    13261 ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct tttaggaaca
    13321 acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat tgccatgcca
    13381 gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga agctgctaga
    13441 atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta cttattacca
    13501 agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt tgatcaaatt
    13561 tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt acttgtttat
    13621 aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt agtcttattt
    13681 atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt tgaaatctag
    13741 gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg cttttattat
    13801 tagggggctt catgatttta gttcccttag tttcaacgct attcggttca tttaaaacaa
    13861 cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc
    13921 gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc atcacaatca
    13981 ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta gcaagaaaaa
    14041 tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga atttttgttc
    14101 ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt ttagcaaata
    14161 tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta tttttatatg
    14221 ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa attgatgggg
    14281 ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca atgcatgcga
    14341 cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca ttattaattt
    14401 taaatcgtga ttctaatatg tggacactac cattgtttca atataattat acgggacaat
    14461 attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc acgattacac
    14521 tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga gcagtgaaat
    14581 aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata tttgacaatc
    14641 acacacaaac atttcattta caaaataaaa aaattagcta tctattagga atcgaagaaa
    14701 ataattattt aacgcatctt tattttggaa aaaagattac gcactattct ggtgggtatc
    14761 gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat
    14821 tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat tttagagaac
    14881 cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct
    14941 acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat acggaagatg
    15001 atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga ttgactttac
    15061 agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg caattagtaa
    15121 ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt gattttccaa
    15181 atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt caaatgacta
    15241 gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca agcagccatc
    15301 aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat
    15361 atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa gatccatttg
    15421 accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa ctaaaaactg
    15481 gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt ctgaatacgt
    15541 tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac tatcgtttaa
    15601 aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt aacgaagaaa
    15661 aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt gttttagatg
    15721 atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg gttgaaacag
    15781 aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat aacgggatga
    15841 aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta tttcgagcac
    15901 atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga aatcaatatg
    15961 ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg acggcgattt
    16021 ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg acagaagtct
    16081 attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat atgttagggt
    16141 tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt gaaagttgtt
    16201 cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa acttggacga
    16261 gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc ttagtgtatc
    16321 caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa
    16381 cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat gagctagatc
    16441 taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc ttttataaaa
    16501 agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg tttgaaggca
    16561 atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc ttttatttcc
    16621 gagtattagc agaagctgcg catccattag tcacattgaa attagcagga ttagcagaag
    16681 aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg aatctcggtt
    16741 tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat ttgaaacaga
    16801 tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt ccaaacgaag
    16861 aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat tgggaaaatg
    16921 aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa ggactaggcg
    16981 gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc atctttgaat
    17041 ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag tcgggcattg
    17101 aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt atgacggtag
    17161 cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat ggctattatt
    17221 cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt gaatggttta
    17281 aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat caattagtta
    17341 cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca cgttcaatta
    17401 ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg gcgacgcttt
    17461 ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga atgaccaaca
    17521 tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag tataaacggg
    17581 cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg
    17641 ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc tcaacttctg
    17701 ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa gctcagttaa
    17761 ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga aaaaatagtg
    17821 tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa aatgaacata
    17881 ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta aacgttacag
    17941 caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc aataattatg
    18001 aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa agtattgtat
    18061 tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc
    18121 agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac atttacaagg
    18181 tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc gaggctttgc
    18241 gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg tggaagcatt
    18301 aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc gtgaatcaaa
    18361 atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa tgtttatccg
    18421 tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt taatctacaa
    18481 gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga cagaagaggt
    18541 ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag ttgtgaaaga
    18601 atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa ttcgattaga
    18661 tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg aaccagatat
    18721 ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc
    18781 agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt attatatcta
    18841 tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat cagaacgttt
    18901 agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata cgcatgatgg
    18961 aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt acacctctga
    19021 ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca attacaataa
    19081 tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata atgaccgtag
    19141 ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa tttattacgt
    19201 tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag aagggcgtaa
    19261 tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac ggccagtgat
    19321 tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg atttggatgg
    19381 aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta aaaataaaaa
    19441 cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta tcattgagtc
    19501 taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc catagaacaa
    19561 aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag gactcataca
    19621 tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata aaattaggaa
    19681 aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt
    19741 cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc
    19801 gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat gtgtcatcga
    19861 ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg ttagaagaaa
    19921 tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag cgaaatcatc
    19981 tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag tgggcgactt
    20041 tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg gacgatacta
    20101 cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc aatcaactac
    20161 cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat taggactttg
    20221 aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt aaaaaaaaca
    20281 ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt ctacgagacg
    20341 agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta gtcactgttc
    20401 aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc tcttcaaaag
    20461 aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag cacatcaaac
    20521 aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga
    20581 ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg aaaacggaaa
    20641 aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc tttgaatgtt
    20701 gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac ttttggtttt
    20761 cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc agcacagttt
    20821 tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca
    20881 ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt aaggagcaag
    20941 tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa cgctactatt
    21001 tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg gtggttataa
    21061 caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga atcatacgac
    21121 aggcaaaagc aattatggtt tcaaaaagct atacctatta cgaaaataga agattaggac
    21181 tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta gaggaggatt
    21241 ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt tactgctagt
    21301 ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag
    21361 ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag aagcagaaac
    21421 ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac cttattatca
    21481 agggaaagta gaagaaagaa catataaaaa tcggttatct acaatcaaaa aaacttttct
    21541 tatttcttta aaatggagtt aagagaaata cgtccaattg acattcaaaa gtggcaaaat
    21601 aatttattga gcaaatataa aaattcttat gtaagaaaca tgtatgggat ttttcagatg
    21661 tcattagatc gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa agttattgga
    21721 aatgtgaaga aaaggaaaac tacagttgat ttttggacaa aagaagaatt tacaaaagtt
    21781 atttcaactt tttatattga tgatttgtac caacattttg gttttatttg tatgtggtta
    21841 ctttttatga ccgttatgcg tttgggagaa gccactgcat taacttggaa agatattgat
    21901 tttgaaaata aaaacttgcg tgtgaataaa acactctatt ataagagagc caaaacctat
    21961 gagtttgtag aacccgaaac ggcttctagt attcgtacaa tcgaactaga cgacaataca
    22021 attacctttt taaaagcttg gaaggagcgt caagtttcaa taggaggcat gtcgtttgta
    22081 ctttcttata atggaacacc tactcagaaa tatacaatcc gtcatattat gagaagacat
    22141 gcaacgatcg ttggtgttca tataatccgc atacacggat taaggcatag tcatgcttct
    22201 ttgcttatat cacaataaaa agatgcactt cgaattaaag aaaggttaaa acatgctgat
    22261 gtacaaacga ctttaggaac ttatggtcat ttgtatctaa attcattgag agagataaca
    22321 aatgatccca cagattatat ccaaactaat ttttctaaaa tcaaaccgac acctatttac
    22381 gataataaaa aataaatgat aaacaaaagt aggatcacac caaaagaaac acactagaaa
    22441 aactataata caggcttttc tagcgtgttg tttcttattt ccgcctattt atacattata
    22501 ctcataaagc atttaatgca tcgatcgtag ccgcattgtc tatatgggta tataaagttg
    22561 ttgtattggt acttgaatgt cccaattgtt gggcagttaa taccaatgaa tttgtctgtt
    22621 ggtagagtct agtagctaat gtatgacgca gtttgtgagg gctgacttga attctaaagc
    22681 cttttgaata ttttgctacc atccgttcga ttgcaatacc ggatatacgc ttggcttctc
    22741 cttggtaaat tgctagaaat aaagcttttt cgttttctgt tactttatat cgttctttcc
    22801 gaatagctag ataattagct aaatattcca ttgcgaatgc tgctatattg acagcatctc
    22861 ttttatttcc ttttctagtt actaccacat tattcttatc taaagatagg tcatctaaat
    22921 taatatttat aacctcggag agtctcaatc cagatcctaa aatcagtgca ttgatcgcca
    22981 ggtctctttc tacatctcgt tgataataga tcaaagctcg tttactcaaa gtatttttat
    23041 attcattggc aatataatct agataatttt gtgtatcctt atctagaaat aattttttct
    23101 caatggcatt ggcacgtgca gctaacgtag ttttatctgt gatggttgct acttgatcca
    23161 taggattatt gtctaagtat ggttttccta gaggattatt ggaacttttc gaaagatatt
    23221 taaatagaac ctttaatgcc gtgatatttc tattgatagt agaagtagat agacctaagt
    23281 tattattatc tttgagctta gtttttgttg tctctttttt tacattttct tttgtgcgac
    23341 gtttcaaatg agatttgtac aagtttaggt cataggcagt taactgtctt atcgaggtga
    23401 tagaagtatc aggaacgata gactcactca gaagccattc ataaaagagc ttataatttt
    23461 ttagatattc taaagtggtt cgaggtgatt gatctttatc ctcacgatct tgaacaaaat
    23521 cttttatata atcgggcata tgaagtatga ttgtatctat tttttgatat atttctgtat
    23581 ttttcatgtc attcatcctt tcaataagta ggggagaaga gtatctctat aattggacta
    23641 taccaattat agaatgtttt gtcaattggt atgctctaaa atttattaat ttgtttcaaa
    23701 aaaagcatat aaacaggtat acgtttaata atttaaaata aatggtatac ataaataggg
    23761 aaggaaatag aagggagcaa tctaaatttt ttgaattttc ttaattaatt atatagaaag
    23821 aacaatcttt ctatagagtt agacatgaga gggactatgc ttgatttaaa atcaacaagc
    23881 gtatatactg tatatacaaa gaaaaaggag ggagaagact atggcaatta aagaaagaaa
    23941 atttagggaa gtggggaact cagtagtatt aactatttca aaggagtttc ttaaagaaaa
    24001 tgggttaaat cccggagata cggttttttt agatgaagaa aaacttcgag cggcaattac
    24061 aaaaaaacaa gccttatctg atgaaattga tttagcaata tctcaagcat taactgaata
    24121 tgatactact tttagagagc ttgtcgataa atgagtattc attacctaga agccaaagat
    24181 attataaaaa tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc ccttggtgta
    24241 aaggatgcga atgctttaga aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa
    24301 gaggtttatc cttcgattta tgaaaaagca gctatcctac ttatacaatt gataaagaaa
    24361 catccttttc acaatgcaaa taaacgtact gctttcttag ctacttttgt ctttttgaaa
    24421 atcaatggac agcttttagc tattgatcaa aaagatgctg taaatttagc ggtatatatt
    24481 gctacgtatg ataaggattt cgatcaacta aaatatgaag tgatcaacac tataaagaca
    24541 catacgcagc ccatttaagg agttactgta tgtgtcttta tagggttatt agctgctata
    24601 gcgaaataat tgaagtaaat tacaaatagc tataattcaa atccaactat atagaaaacg
    24661 taaattttct atatagttgg attaagataa cgactattta ttttatcata tttttactat
    24721 accctatact tttacttggt atatttatga ataatcgcaa atttacccct atactactag
    24781 tttgtaagtt agattaataa aaatactaaa gttatttgtt cttttattaa atacacctac
    24841 aacttataaa ttgatggtat ttagataaca cgaaagaata aaaataacag ctgatagtaa
    24901 agtttgttga cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga
    24961 aaacttatga aatgaaagac cttttacatc ttgcagaaat cgttactggg attaaactaa
    25021 gcaataaaaa ctaatgttcc cctcattctt tgaacttcta ctatttgatt tatttattct
    25081 gataaatgac gatttcacct tcaggatctt caaatcccgt atagaattta tttaagatct
    25141 cttcagtatc agtaatagga taggcgatag gccacatacc ctttgatggt tagaataaac
    25201 tgctcaaaat ctgtatcgta tgttggcttt agcatattaa caaaggctat ctccaaatta
    25261 taaaccataa gcgttgaatc gttcttaaca atttcaattc ttcctaaccc aaagtcagaa
    25321 gatataatta ccggacttgc cttacccttc atgtgggttg ttgagattcc atatttaaag
    25381 ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa atctttcccc
    25441 tgctagtctc ctaagtccct ttgtagcgga tatggcacca taatctcatt ttatcgaagt
    25501 gagtcatggt ttatctgttg gccaaccaaa attgatacaa tctaacgatg caccctctac
    25561 ggcattctag gggagtgcac ttgcattcac ccaataaaaa ccggcagtta gcaaagaact
    25621 tatgtagtcg tatgttctta ctgttaacat acaagaggtc tcctttccac aattgcttag
    25681 cagtaaacgg gaatttacaa agctaatctc ttccattttc ttatctttgt tctaaaagtc
    25741 ccaaatggag caactgtatt tacatgaata aatttatata cttcccatac agccgttttg
    25801 gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata gatcttcatc actcattgaa
    25861 tcaatcatca tgttaatatc atcaatatta cgattgagaa tttcctctaa ttctttcaat
    25921 ggtagatgtg cataggtatt tgtaaaccac ttatataact ctcctaactg attccattta
    25981 aattgatcag aaggcgtttt aacagataat ccatttcttt catcattttc ccattttaga
    26041 attaaattcg tccaaccaac ttgatatgct aaattttcag caggtgttct atccacttcc
    26101 tcaactcttt tatccttcaa ttcttcagga atagtttcaa attctaaaat atatttagta
    26161 taagtcctat ttatttcttt ttttaattct tctttatttt tatatgttct cattatatta
    26221 cctccataaa ttcctaattg tctcctttaa ttcatctata atttcatcca cgatatttta
    26281 atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg tgaatgattt
    26341 ataaaatatc ccaacgtctc atctaaagaa atgttgatcg tcagcaagga gctaaaaaat
    26401 acgaatatat ttcttttgta gtaaaatcgg actggtttgc atatcatttt cttcgtaaat
    26461 atcaaaatca tgttaactac tatcaattac taataaaaac taccacttta agcgttaaga
    26521 actgttaagt ttagttaagt gaggatagtt cacaaaatcc attgttaaac ttcacgaaag
    26581 tggtaaaact caaaatgctt tatataggat tacagcatcg ctctttcttc gattgcgcgt
    26641 tgggtaaaaa atattatgaa gtaaaaattg acgatgacac gttccttacg gctcatcaga
    26701 tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat
    26761 tgccatattc acgcctcgct cgacgaacta ttaaaggtca agatttacct gaagatcatc
    26821 ctttttagga aattgaagct ttttatgttg cacaatgcat ttataatact acaatgatgt
    26881 tttcaccaga tattcttcta gggtgcggtg taatgaaaca gatacacttg caaaagaaaa
    26941 ttcaccgcga atttactaaa attatgaata attatgttaa tcttccgaaa tttttttcca
    27001 aaattaaggg atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa ataacttctt
    27061 tttaaacaat tatagaaaac cgatactctg ttaagttggt tgacacggtg tcgattttta
    27121 tgactttctt attctgaaac ttcactaacg gacagtacct aaaaagtcta tagtatggac
    27181 gtttacgagt actattttat caaaaatgct gctcatttca acttatatgt tttagtgaac
    27241 tacccccact tagc
//
LOCUS       DAZKNM010000016        27174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000016 DAZKNM010000000
VERSION     DAZKNM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.016"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..1616)
                     /locus_tag="ACG8M9_000598"
     CDS             complement(48..1616)
                     /locus_tag="ACG8M9_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0377392.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1620..2030)
                     /locus_tag="ACG8M9_000599"
     CDS             complement(1620..2030)
                     /locus_tag="ACG8M9_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0377393.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2027..2950)
                     /locus_tag="ACG8M9_000600"
     CDS             complement(2027..2950)
                     /locus_tag="ACG8M9_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0377394.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLLDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2947..3825)
                     /locus_tag="ACG8M9_000601"
     CDS             complement(2947..3825)
                     /locus_tag="ACG8M9_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0377395.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3812..5149)
                     /locus_tag="ACG8M9_000602"
     CDS             complement(3812..5149)
                     /locus_tag="ACG8M9_000602"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0377396.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5152..6255)
                     /locus_tag="ACG8M9_000603"
     CDS             complement(5152..6255)
                     /locus_tag="ACG8M9_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0377397.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6329..7837)
                     /locus_tag="ACG8M9_000604"
     CDS             complement(6329..7837)
                     /locus_tag="ACG8M9_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0377398.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7803..8495)
                     /locus_tag="ACG8M9_000605"
     CDS             complement(7803..8495)
                     /locus_tag="ACG8M9_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0377399.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8517..9491)
                     /locus_tag="ACG8M9_000606"
     CDS             complement(8517..9491)
                     /locus_tag="ACG8M9_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377400.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9791..10663
                     /locus_tag="ACG8M9_000607"
     CDS             9791..10663
                     /locus_tag="ACG8M9_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377401.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11121..13319)
                     /locus_tag="ACG8M9_000608"
     CDS             complement(11121..13319)
                     /locus_tag="ACG8M9_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377402.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13551..14543
                     /locus_tag="ACG8M9_000609"
     CDS             13551..14543
                     /locus_tag="ACG8M9_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0377403.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14603..16024
                     /locus_tag="ACG8M9_000610"
     CDS             14603..16024
                     /locus_tag="ACG8M9_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0377404.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16017..17516
                     /locus_tag="ACG8M9_000611"
     CDS             16017..17516
                     /locus_tag="ACG8M9_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0377405.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17555..18949
                     /gene="uxaC"
                     /locus_tag="ACG8M9_000612"
     CDS             17555..18949
                     /gene="uxaC"
                     /locus_tag="ACG8M9_000612"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0377406.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19108..19791
                     /locus_tag="ACG8M9_000613"
     CDS             19108..19791
                     /locus_tag="ACG8M9_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0377407.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19793..20629
                     /locus_tag="ACG8M9_000614"
     CDS             19793..20629
                     /locus_tag="ACG8M9_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0377408.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20640..22394
                     /locus_tag="ACG8M9_000615"
     CDS             20640..22394
                     /locus_tag="ACG8M9_000615"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377409.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22670..23011)
                     /locus_tag="ACG8M9_000616"
     CDS             complement(22670..23011)
                     /locus_tag="ACG8M9_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0377410.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23079..25250)
                     /locus_tag="ACG8M9_000617"
     CDS             complement(23079..25250)
                     /locus_tag="ACG8M9_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0377411.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25461..26318
                     /locus_tag="ACG8M9_000618"
     CDS             25461..26318
                     /locus_tag="ACG8M9_000618"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0377412.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26389..>27174)
                     /locus_tag="ACG8M9_000619"
     CDS             complement(26389..>27174)
                     /locus_tag="ACG8M9_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0377413.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         8068 a         5608 c         4884 g         8614 t
ORIGIN      
        1 taactagtga aaataagtta gtgaacctca tttattaaag aattaaatta cagatgatct
       61 tttccatcat acttgaaaaa ctcaaaatca gcataacttc ctcttttttt atttagatca
      121 tgcactgcta tcccgataaa atttccagta aaccctcctg agagtataca catgtttttt
      181 tgcggaccta ctttcttcca ttcttcagtt gatacgtctt tcaaatgaaa ctgtgctgtc
      241 tctttcttta cagtgacttt caaaaaagtc ttcttttctg tgagtggaat cgtccaatct
      301 tctatttgtg ctactccgtt ttctgattct atcatacgta ggcattttcc cttgtcctca
      361 ttccaagtaa cataacaata aatataattg gattcattta agtacagtac cagtccagcc
      421 atctgattaa aattattagg catgtattca aatgctgtct ccgcatcaaa agtaaaatcc
      481 gtttgccgaa tagctaacaa atgatgttcg aaggtagatt gaagagattc accggaaatc
      541 agacgtaaaa aacctggtct acattttgta tcacaccaat cagcatttgg caaaatgcgt
      601 ctggcattcc agttcttctt tagctctcct tcaaatgtat ccataaactg atgatctttt
      661 tcttgttgta cctcttcatt tgtctcaatc attatttctt ccatcggagt tgtctggcta
      721 tctttaagtc ttaaccaatc gtcggacgtc caatacactt cttgaatagc ggtttctctt
      781 cccaaaatag ccgcaccgtt taatggtctg gtacaaagat atgtcatgta ccatcttcct
      841 gacggggttt gtactaagct cccatgtcct gtacattgca atgtagaagc aggtttgtca
      901 ctagctgtca gcataggaaa accaggatca agctcgtaag gaccagtaat attctttgct
      961 cggcaaactg taactgaatg tcctcttcct gtaccgcctt ctgccgtcat caaataataa
     1021 tacccgttac gaaaatacaa atgtggtgcc tccgttttcg ctaattctgt acctgcaaat
     1081 attttataga tgggaccatc aagcttcttc ttctctgcat catattcttg taatacgata
     1141 ccgctggatt tattcggtgt gtccagacga taatcccata gttcattgac aagccatttt
     1201 tttcccgttt caagatcatg gaacaaagaa ggatcaaagc cgttactatt taaataaatt
     1261 ggttcggacc aaggacctgt gatctcagat gcagtgatca aataattgtg gacatcttta
     1321 aacggacggc ctgcactttt gacatctgtg taaacaagat aaaacaatcc atcagcataa
     1381 ctgatctgtg gcgcccatat acttccattt gtaggatttc cttgcaaaga tacttgagtg
     1441 gtcaaaatat ccgttgtatg tgaccagttg actaaatttt tcgactcata tactcggatt
     1501 cctggaagcc attcaaaaga ggagacaata atgtaatacc gctctttcac tcttaatata
     1561 ttaggatctg gattgaatcc tcttaaaata ggatttttca ctttgatagt ctgcatgatt
     1621 caggctcctt ttctcattct tactcgatat aaactgaatg ccccagctgc gaaataaaaa
     1681 aatgcaaaag gaaacgcaat aaactgagtg ccaatcaaca atatgaatcc tgttataaaa
     1741 tacaaaccag tggcagttgt taagtatttt cctttagcag tcaaaaacaa tgctactgct
     1801 aaaaaagcaa gtgaaataaa caacacaacg gcaaacagac cagtcatgtc ctgtaataat
     1861 ccaaatgtat tttctaaaga caaagttgaa tgatctacag caagcgactg ataacttttt
     1921 ttaaactctg acgcagacat attacttaaa gtaactgcta acccgccaaa gaaaatcaaa
     1981 gatagactgc caccgaccat tcctagcaat agttcccatt ttcttttcat ttctgaaccc
     2041 cctgcttttc taaatgtatc cattcaggtc ttttgatcga tgtttcataa ttgttattac
     2101 gttcagagaa tttgacagtc tgttcatttt tcttgtcatc ccaattatcc caaccagttg
     2161 ggtgaacatg cccacctatc ttacacgctt caaataagac atttgcatac ggacgccacg
     2221 gtcttcctaa atagacgttt ctagtatcag gttctgcagt caaggtacag ctttcaaaac
     2281 gataccctgt tttttgattt ttcggcgtag atgctgctgt gatgtatcca ggatttttac
     2341 tggctaagct atggatatga cactgatgaa aaacggcatc tgccccacca aatatgaaat
     2401 ccaccgttcc ttcaatccag caatattcat aggtctgacg acaatactca ggattggcag
     2461 aagccggtgt acaaaaagga gtaccatctt tttgcaaatc aagcaacgga cccgtgcata
     2521 aggtatcctg ataaccagat agtcgacagt tttttaaaga tacgcgatct cctgaattga
     2581 ataaagcaac agcctgtcct accttttctc ctggacctgc tgtattttcg atgtgaagat
     2641 tttctaatac gacattcgat ccttctaaga aaaaagtagc acttcggaaa gtccctaact
     2701 ggctaccgtc aagatactcc tgttttgcaa aacgattacc ggagataact actgttcctg
     2761 ctcccacaag tttaaaattg gataatctgc tttctatata ttcatcatat cttccttctt
     2821 ttactatcaa gacctttgga tggctatccg gcaggcctgc caaataatcg attccttctt
     2881 ggatagtttc aaaatccgtc ggggtatcta tagcattctt tccaattatc agtttgtctt
     2941 tcatcctcat aaatgctccc ttatgaattg tttccaaata tttctcattt cttgtgtttc
     3001 catatgaccg cctgttaaag aaaaagaaga aaagttttct ggactaccca ttgattgata
     3061 ctttttgtcc agcccttttc tcagcttcag tacgccttcg tccaagcaca tcgtatcgtt
     3121 ttttccaact aagctaagac gagggcgcgg agcgattttt tcttggattg ctaacgttgt
     3181 ataagctgtc aatagatttg gcacatagta ataaaaccca tgatgatcta ggcggcgatg
     3241 tagcaaaagt atttcgatgt ccacctgtgc cgctagatca atacaaactt tgacacgaga
     3301 atccaatgct gtcacccacc agcttaataa gcctcccata gaaagaccaa tcgttgcgag
     3361 ccgttgagta tctgtgtctt cccttgtctc caaataatcg atcaagctga tcacatcata
     3421 aatacgcatt ccccataacg tttttcctgt taataaaaat tgtttgaaca actcgctttc
     3481 agaaatacct ccgcgttctt caaatcccca agcatcaatc gaccaaacag catatcccat
     3541 tcctgtcagt acttctgcaa aagatggaga agctaagtaa ggagcaccat ggatcaattc
     3601 acttttcccg actgaaaaat ctccgccgtg agaatgcagg taaaggaccg gcggaaacgg
     3661 tccttctttt tctggataag catagagggc tggtactgtt tccaattcat ttaagtacaa
     3721 ttgatagtat tcaagcataa aaccgttttg tttttctcgg tataatcgtt ttccactagg
     3781 ggtatgtctg ctagatggag cacctaaaaa atcattcaac tgcataagca aagtttttac
     3841 gaacaaccag ttcatccata ttttctgatt gatttgattg gaatttctca atactgattt
     3901 ctttagtatt ttctaataga aatgctggtc cctcgtgatt ttcaatcgac atctggttaa
     3961 aggaaacctt acccgcaaag ccaatataaa atccttgtct gctcatttcc tctattcctg
     4021 tcatcatagc aggaacaccg ggaactgcat cttgggccat cgagacatcg atattgtcaa
     4081 atgtgatttc ttgtatgaat tgttccgcta atccataaat aaaacctgca gaagcatgga
     4141 cattccttgc cgtgatattc gaaaaatgga tccttctgaa tgctggtgtc cgttcatcta
     4201 tcggataagc ctttttttcc catacatacg gttctttccc tcgcggacca cagaaataat
     4261 aaagattcag tataaatgga cacatcacat tatccatgac tatattggat actcgaatat
     4321 cttcaacgat cccgcctcga cctcgtctag atttcaatcg tattcctcga tcagtttctt
     4381 ggaagataca atttgaaatg gtaatattac gaatactacc actcatttca cttcctaata
     4441 cgacaccgcc atgtccatgg accatcgtgc aatttgtgat cgtgatattt tcgcaagcga
     4501 tcctttcgta cgtatcttca gtccctgcct tgattgcaat acagtcatct cccacgtcaa
     4561 tatggcaatt actaatacgc acatttttgc atgattctgg atcaattccg tctgtatttg
     4621 gtgaatccgc tgggttcaag atcgtcaagt tatcaaacgt tgcattactg caaagaatcg
     4681 ggttgatcgt ccaacttggt gactggatca atttaatatc tttcacagta attcgatgac
     4741 agttatggaa actcatcaat ttaggacgag gataagcgag attatctggt tcatttctaa
     4801 aagtatgcca ccattttttt ccatttccat ctaatgtacc gaaaccagtg acagagatgt
     4861 tctctacatt ttgagcatag atacaggaag cataaacttt tcgatggact ccttcccaac
     4921 gtgaatgaac aacaggataa tcttttggat cgtcagagaa tttcagaacc gctcctgcgc
     4981 ttaaatgaag ttcgacattg ctttttaaaa ataaagctcc tgttagaaat tcacctgctg
     5041 gaacaactac ggtcccgcct ccgtcacttg ctgcagcatc aatggcttgt tgtattgctt
     5101 cagtattcaa ttcatcaatt gatgcgccaa atgtcaaaat atcatacata tttataatgc
     5161 ctccacttca atcaatgctt gcaagaatgc accaacgcct ttttgatcat tacaaataat
     5221 tggttcactg atgtagtaag catatgaacc atctcgctga tctgctccgc ctaaccctgc
     5281 tacttgacag tttttattca agttcagcca gccttctttt gtttccagaa caaattcatc
     5341 aagtaatcct tgatgtgatt tcttcacttg tggaagccat tcttctttgt tcagcagtcc
     5401 caaacgtatt cctttagcca ttgcgtaaac aaacatacta ctgccggaag cttccagata
     5461 atttcctttc cgttttgttt ctgttgttac ttgataccat gtcccgctgt tttcttcttg
     5521 atatggaagt aatgcagcca gtgtcttttc tagaatttct ttcagcaaag aacaatctgt
     5581 ttcttcatga agtaattcat aggtatctgc tgcagccatc aaataccagc caatcgatcg
     5641 gctccaaaaa tgatgggata gacctgtttt tggatcagcc catggttgga ccttcttttc
     5701 atcccatgca tgaatcagca aatttgtttt tggatcgaat agatgtttat aactgatttc
     5761 aaactgtctc aaaacatctt ggtaatcttt ttcttcacta aaattctgga taaattcagc
     5821 ataaaaagga gaacccatat ataagccatc caaccaaatt tgataaggat aaatttcttt
     5881 atgccaaaaa gctccttcgg atgtgcgtgg atggtttgcc aactgctggg aaaggagttc
     5941 tgctgctttt ttatattttt cttcgcctgt ttctttgtac aataaaaaga aaagctttcc
     6001 tgtatttaca tggtcgatat tgtattcttc aacgctgtag ccacggatcg ttccatcttc
     6061 ttgcacgaaa tagtcgatat tgtcttttat gaaatcaaaa taaatttttt cttgggttcg
     6121 ttcccaaagt aattggaacc ctttaagtac gacgccatag tcataagacc attttccttt
     6181 ataccacttt tcttcatata agcgaggtgt tctttccatg atactttttg ctaatttctt
     6241 tgaccagttt tccattattt gctctccttt aaaaattaaa ataggatttg ggacaaatac
     6301 caagaagtat ttgtcccaat cgtctatatt actgtgccgc tgctttgtat gcttcctcgt
     6361 actcttgtgt gattcgatcg cctccttgtg ctttccagtc ttctacagct tttttgaatc
     6421 catcttcgtc aagctgtccc atgatgtatt gcaccgtagc atcaatgacg attttttcaa
     6481 tttctgatcc tacttcattg ttcgtcggtg aatccaatgg cacagaagga tccattacag
     6541 caaattcttc attttctttg atcaattgat tagagaattc tttttcaggg tctgtatctt
     6601 tgaaagagaa cgtcacttca ctaggacgag agcttgataa cggttgcacc tcttgttgcc
     6661 aaagatccat atcgataaat tcgatagaac catcatcatt caattcataa tgcgttcctt
     6721 cgataccgcc agtcatcaat ttatacacat cttcttctaa aagatcatca acaaattgaa
     6781 gtaatttctt caattgtgct tcgtctttta cttgagattt gggaaatgct agcaagccgc
     6841 caataccatt tccttctgac caaacatgat aaccgccgcc ttcaccatct gagatcttgt
     6901 ttacaggcac taattccata tcatcttgaa taccttgacc catcgtcttc agattcttga
     6961 tatccaccat tccagtgtag attccagttc ttccttgtgc aaattgttct tgctgatcag
     7021 tcttttgggt aactgcaaag tcttgtgcca aatagccatt ctcaaataga tctcttgaaa
     7081 aattcaaagc tttcatatac tcttctgtat caaattctgg agtgaatttg ccattatcgt
     7141 ctacagccca gccatttggt gctccaaaat aagaagtgaa aagtttaaag ctggtataac
     7201 gaatatcact aggcgcacga tcgccaaaac caactgtgtc gtccacacca tttccatcag
     7261 gatcgttttc tgtaaattgt ttagctactt cataaagctc atccatggta gttggaacat
     7321 ctagtcctag attatccaac cagtcttttc gaatgactaa gccagcacga gcgtagtctt
     7381 tttgtatcgg tacgccgtac aaggcaccgt cgatactcgc agcttcgatt ctatcttcag
     7441 aaatttgact taaattttta tagtctttta aatattctga tacatcccag aatacgcctg
     7501 attttaacgc attgcggaca gaagagtttt tcaacatcgc aatttgaagt gtaacgatat
     7561 ctcctagttc atcagaagcc aatgcagttg ttaatctttc ttcttttgaa gcatcaggaa
     7621 tccagttgaa tgtcagttcc gtatcggttt tttcttcgat caagttcaca atcgtgtctg
     7681 taggcggtga agcatgatgc aaaatattta accaagtgat tttagctttt ccatcttctg
     7741 tctctgaaga actgttactt gaactgctgc cgcatgcggc aagtgctaga attcctgcaa
     7801 aactaagacc taattttacg agcgttgctt ttttcataaa ttttctcctt cactgtttta
     7861 ataatatttt tgttttcttc tgttttttct gtatatgagt tcttccattc caaatggcaa
     7921 aatatagaat ttgctgtcca cgtgactgcg gaaaagaaga gactgccacc gataaaggtt
     7981 aaaacacctg gcattgtttg gatgatcatc agataaatcc ctaccatact taatatcaac
     8041 actaatgagt aatgaagatg tgagaatccg aataaaaatg ccattttcat tttgaaaaaa
     8101 tatcctttcc aatcataatg agccatgatt gggaaaatat agagtgctga gagcacatat
     8161 atgaacgcag caatatacaa agcgatccta agataccaat tgtgtatgaa agaaagatcc
     8221 acaagtaaaa caatcccgat ggcgccatat atccagctca tcagcaaggt ttcacgatag
     8281 ttttctttaa aatatgtgaa gtacgttttc gtcaaaggaa acgtttcttt tgagcgcagc
     8341 cattttctaa tcactgcaac catcgcatac gatgcaggac cgaaagtaaa aactcccaaa
     8401 cctgcgatga ctaaaccgcc ccacagaaaa ttcaaataaa ccaactgtaa aatacgattt
     8461 agccttccgt ttattttatc taatgactgc agcatatctt tccctctctt tctatcttag
     8521 taaacgccgt cttctcctag ctttttggca atcttattag ctaaaattac cataaacaat
     8581 cctacgaccc ctttaaacaa tcccattgct gtactgaaac ttaactgtcc attcttcaat
     8641 ccagctgtat aaatataggt gtcgaaaatt tctgcaacgc ttcgattcaa tgaattcaat
     8701 aacaagtaca tatgctcgaa ccctaagtct aatgtctgac cgatttttag aatcagcaag
     8761 gtaatgatta ctggacgaat tgccggcaat gtaacatgcc atgttttgcg taagcgtcct
     8821 gctccatcca tttctgcagc ttcatacaac tgtgtatcga ctgcagttat cgctgaaagg
     8881 tagatgatcg tagaccagcc caactctttc cagatcactt gtccgatata tactgtgcgt
     8941 gtccattcag gggatgttag aaaactgatt ttttcaaatc ccattgttgc aaggaattca
     9001 ttcaataccc ctccgtcaac attcaaaaat acataagtaa tcgaaacaat gattacccaa
     9061 gacatgaagt gagggatgta aatgatcgtt tgaatcccgt ttttcaatcg tttgttcgtt
     9121 acttcattta atagtaaaga caaaatgatt ggcatcggaa aaaacacgaa gatatttaat
     9181 ccgaacaaga tcaacgtatt tcttaataat gtaaagaatg tcggttctgt aaacaaacga
     9241 atgaaatgtt tgaaaccgac ccaaggactt cccattattc ctaaaaatgg ttgataatct
     9301 tgaaaagcaa taacgagtcc ccccatagga agatacctaa aaatcaagaa ataaaggact
     9361 cccggcagaa tcatcacata taaaaattta ttcgttttga ttcctgcaat tatttttttt
     9421 cgtcttctga gcttttcttc ttttttcgat agcgtcagtt gaaatggttc ttgtttcttt
     9481 acactttcca taagcccctc cttagttttg tgaatgtttg ctgcttatga cttcatgata
     9541 gataactatc tgaattacgt atataatcat ttttaccgta agtaaaaacg cttacatagc
     9601 aatgattttt gtgctataca aaacgataaa gtgtattttt tcacaaataa actacgcata
     9661 gcaaaccttc tatttttttc tatctgacac ttagcatagg aaatgattat atacgtaatc
     9721 tttcaccgat gataaattga acatatagaa acagaaacat ttaatttatt ttgaggagga
     9781 gcctatctat atgaaaaaga aagtctctat aggagaaaga agttttacac ttttcaacaa
     9841 tacgtttcta attttgctag ctttgatctg tatcgttcct tttttaaata tcatcgctac
     9901 atcttttgct tctacacaag aagtcgtcgc gaaaaagttt atcctttttc ctactacctt
     9961 ttctttagac gcgtatcgct acattctttc tacgccgacc atttttagag cacttgctgt
    10021 ttcgattggt gttacaggtg tcggaacgat cgtcagtatg tgtgcaactt cactgatggc
    10081 ctacggtctt tccagaaagt atttgtttgg tcgaggtttc gtcaactttc ttgttgtatt
    10141 ttctatgttg ttcagcgggg ggatgattcc aacgttctta gttgttcgtt ctctagggct
    10201 ggtcaattct tactgggcca tgatcctgcc tgtggctgtt aatgcaatga atatgatcat
    10261 tatgagaaac tttttccaag ccttgccgga tagtttggaa gaatcagcaa aaatggatgg
    10321 ttgcacagat tttggtgtat ttttcaaaat tatgctgcca ttagcgttac cgtctattgc
    10381 tactatctcg cttttttatg ctgttactta ctggaacact tatatgactg ctattttgta
    10441 tatcaacgat tcatctaaat ggccgataca gattctttta cgccaaatcg tcattgtatc
    10501 aagtgggatg caagcagaaa gtagtgccgt tgatgtgatt ccaccagcac aaacagttaa
    10561 aatggctgtt atcgttattg caacagtgcc tatgctgatt gtttatcctt ttgttcagaa
    10621 atattttgtc aaaggagcac tcgtaggttc tgtcaaaggg tgattatgta ctgaacgcaa
    10681 aatggtagtt gtgtcagaag tggtcagctt caagaaataa gaaggcattt acgaaaattg
    10741 cttcttaaat ttttgtgaaa tttcggctta tttttcgaag aatctactcc tggaacaccg
    10801 tgtatcagag gttgtgaaag agacgttcag ccttgagcga tgataaaaac tctcacaaac
    10861 attgcgacga gtaacccaca ggagcaataa gaagcgattt tgaaaaatag cttcccatat
    10921 ttcacaaaat tatgccttat tgtcgatagc ctgtctcttg aacgccattt attcgtaaaa
    10981 aagaactgtg acaagacttt tgtcacagtt ctttttgcgt gcttagcctt gaaatttaac
    11041 tatccataaa tacaaatact agggttattc taacttcttt tattgcttga atcagtccac
    11101 aagaaaaaca gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat
    11161 tccttctttt taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca
    11221 atctctttta cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga
    11281 ttttgaacaa aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag
    11341 ttagggttat aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct
    11401 gtgtagacga tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct
    11461 tgagagcatt ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg
    11521 ggatgataat tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa
    11581 tctactccca ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact
    11641 tcccgactca aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa
    11701 ttcttcactt cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc
    11761 ggatatctaa taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt
    11821 gaacttggac cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc
    11881 ttgctttcat tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag
    11941 tattctttta cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct
    12001 gcatcatctt tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc
    12061 agcattcgat tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc
    12121 aaatcacgct ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa
    12181 tagcagtcat tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt
    12241 tctactcgat ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta
    12301 actaagtgtg cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc
    12361 aaatctagtt ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga
    12421 gaaatcggac gggcaaatct gtccgaaaat aagtaggaaa gaagaccaat gagtcctacc
    12481 agtgttaaag acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca
    12541 ctagagtcaa cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac
    12601 gttttatctt tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat
    12661 acagttggtt gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt
    12721 tcaatagaaa gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct
    12781 atcgtagcaa ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt
    12841 tcgattcctt tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt
    12901 tgatactttt caatttcctc ttcggtcagt tgactcaacg atccattgat tactccccat
    12961 ttaccagcca aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa
    13021 tttagttctt tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg
    13081 aatatgtccg agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta
    13141 attttccatt caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa
    13201 ctcgattcga tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt
    13261 acggctccta ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt
    13321 ttcttcctct cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat
    13381 ctaattaaat gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt
    13441 aatttttaac gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt
    13501 aatatgatga atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga
    13561 tcaaagatgt cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata
    13621 acagcagttt aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga
    13681 attattcgcc aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac
    13741 tatttttctc aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag
    13801 gcatccattc agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg
    13861 atttaacaga aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg
    13921 atgatcagcc tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc
    13981 gtcacatcaa tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc
    14041 aggcagtaga ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga
    14101 aaaattttcg ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga
    14161 gcatccctgt caatccagcc tattttttaa caggagatta cagcttagaa agcggcctcc
    14221 aaaacatgga atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg
    14281 acgacatagc gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga
    14341 acatttctct gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta
    14401 ccaccgtacg taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt
    14461 tgatcgaagg caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc
    14521 gtgagtcgat caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc
    14581 ataacacaga aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga
    14641 agcacctgta aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg
    14701 gcagattcag cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc
    14761 tttagaacaa ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt
    14821 acaaggactc agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca
    14881 aatgatcaat ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt
    14941 ggcctatatc gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt
    15001 aacagatact cccccacaga gcctcccggg caaactgaca gcctttttat accgacgttt
    15061 caaattaaac aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga
    15121 acgtctaaaa gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt
    15181 tgagtggtta gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg
    15241 ttatcctcgc gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat
    15301 ggtcaaagca gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca
    15361 actaccattc aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg
    15421 tgaacgtaag gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt
    15481 ggcggaattg gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga
    15541 tcagttattc aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat
    15601 ttatgctgac caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc
    15661 gatttcatta aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta
    15721 tatcgaagaa caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct
    15781 tatgtatcga ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac
    15841 tgatgcttgg tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg
    15901 gggagaagat ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa
    15961 gatcgatcaa aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc
    16021 ttgaaaccct gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa
    16081 cccctattcc aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag
    16141 atatcccgca aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca
    16201 aatatggcta tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca
    16261 ctcataatgt aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc
    16321 atccaagtca ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa
    16381 aagtagggat tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg
    16441 ctgaactgat cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata
    16501 ttacgatttt aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc
    16561 gagaaatttt agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat
    16621 taggttgtga gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag
    16681 aacgagtgaa atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc
    16741 tattagaaga gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta
    16801 agttgaatgt cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc
    16861 ctttacttgg tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag
    16921 aagtgccaga aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag
    16981 tttttgactc aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg
    17041 aaccagttta cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag
    17101 ataagtcttt aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat
    17161 atggcgaaaa aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg
    17221 tagctgcctc tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag
    17281 ggactccgtt tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg
    17341 aacgcaaagg tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag
    17401 taatcttaga atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa
    17461 atgaacaaaa tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt
    17521 cataaaacag tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt
    17581 aaaagacgat tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga
    17641 ttatcactgt cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac
    17701 agaagcttgg ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc
    17761 tgaagaaaaa atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac
    17821 cgtaccaaaa attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt
    17881 cttcggtatt gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa
    17941 tgaaattttg gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga
    18001 agtgatttgt acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca
    18061 tgaagaacca gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga
    18121 aaaagaagga tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac
    18181 cacctatcaa gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg
    18241 atgtcgtgtt tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt
    18301 attagaagag atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga
    18361 tgcttatcgt acagaaacac tgatacattt gacccacttc tatcattctc ataattggac
    18421 gatgcagctg cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg
    18481 gcccgatacc ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt
    18541 attgaaccgt gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc
    18601 gaatgactat cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa
    18661 actgcagtta ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt
    18721 gacacaattt gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag
    18781 aagtttcctg tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg
    18841 cgaaatggtc atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca
    18901 agcgattagt tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag
    18961 taacagtaac gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta
    19021 gagaagaaga cggaccaaat ttgctccgtc ttcttctctt gctgttctgc ttcattgcct
    19081 ataaaccttt atgaaagaag agataacatg cctactatcc atattgccgg tgattctact
    19141 gcggcagaaa aagatgaaaa caagcgtccc gagacaggtt ggggagaaaa aatctctgct
    19201 tatttttcac ccgagatcca gatcaataac caagcaaaga atggacgcag caccaaatca
    19261 tttatccagg aaggaaggtt agcccgtcta gcttcacagt ttcagccgaa tgatttctta
    19321 tttgtacaat ttggacataa cgaccagaaa atccaagaag aaagaggtac gcttcccttc
    19381 ggtagctatt taaaaaatct gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa
    19441 atggtcttac tgacatctgt gacgcgacga gattacttag aaaacggaac attgaatcca
    19501 gatatacttg gagactatcc aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct
    19561 cttttagatg tgttctcaag atcacaagaa ttgttccaga ccttctcaaa agaagagaca
    19621 aaaaaattct acctgcatct tatacctgat acttgtaaaa attacccaga aggactaaaa
    19681 gacaataccc actttagtcc agaaggcgct gataaagttg cccgaataat tgttgaattg
    19741 ataaaagaat cccgtctgcc tcttgctaat tatttacaaa aaggagtttg agatgtttcc
    19801 attaaattta ggcatccgtg cccacgattt agatgttcga aatcgtgaag aaatcgttca
    19861 aaaagtatca cagcttcact tgtcccatat ccaatttgca ccacaaaaag catttcctga
    19921 acaacttaaa ttaactgaca tgacacttgg gagtgctgcc tattttggtg aatacttcca
    19981 aaaaaacaat atcgaacttt ccattttagg atgctatatc aacctatcca gcaaaaatca
    20041 agaagttcgt gcacaagcat tggcaacttt caaacagcaa attgctctgt gctctgccta
    20101 tcaagccaag atgattgcta cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa
    20161 ttttactgaa gaagcttatc agatcgccag aaattctatc attgaattag tcgctcatgc
    20221 agaaaatttt ggcgtaactg ttgctgttga agcagggatc aatcatccaa tctataacta
    20281 tcagttagct aaacgtttaa tcgatgaagt agcttcacca aatttaaaaa tcattttaga
    20341 ttgtgcgaat ttgatgcata aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc
    20401 cttggacaat ttacatggtt atattacagc gcttcatctc aaagactata tcatggaaaa
    20461 tggaaagatc cgcatggtac ctgttggcaa gggctgttta gatttccgcc cggtactgca
    20521 ttatatcaaa tatgagcgtc ctctaatgta cgccactctt gaagctactc ctgagattta
    20581 tgttcctgaa gcaatagaac acttgcagtt tatttaccaa gattgttaag gagaagatta
    20641 tggaggaacg aatacagaaa agttatcaaa aatatcatgc aaatgctttt cagacattgc
    20701 tgcatcttta tcagggaaat tattggcgtt ttgccatgtc aggactcttt tttattatca
    20761 aacatcttcc atcttggtta atgccgatcg ctattgccaa tgtaatcaat gcagcttcat
    20821 cgaaagattc ttctcagttg caggccattt atttcaacgc ttttttgatg gcttgtctga
    20881 ttggtcaaaa tatattgacg aattattttc atgtaaaata ccatagcact tcgatccgtc
    20941 aagtagaagc tggactaaga gcgaccctta tcaagaaaat ccaagaacta tctattcctt
    21001 atcaaaaaga attgcaatct ggacgaatcc agtcaaagat catccgtgat gtagaagcta
    21061 tccaaacgtt gtcctctcaa gtatttgtca gcggattaaa tatcgctgtc aatttattcg
    21121 tagctttagg tatcacgctt tataaaagtc ctgttgtttt catgtttttc ttattgactg
    21181 ttcctctcgc cgcattcgtt actctctttt tccgtaaaag gatcagacag tccaactcag
    21241 acttcaggca agaaatggaa gagacttctg caaaagtgat cgaagtgatc gaattagcac
    21301 cgattgctcg agctcatggg ttagaaaaac acgaaacaac tcgcttaaat aagcatttta
    21361 ctcaaatcta tgaaaaagga ttccacttag atatcctgca atctgttttc ggatcagtca
    21421 gctgggctag ttttcagata tttcagctga tttgtttgat tttcactggg atattagctt
    21481 tacaagggcg catccaaccc ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg
    21541 tcaattccgt ttctggaatg atcacgctga ttcctacgat ttcaaaaggc atggaatcta
    21601 tcacttctat tggcgaactg ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa
    21661 aaatccaaca tttggatgga aattttcgtt ttgaggatgt ttcttttctt tatccggact
    21721 cactcaaacc gattatcgat catttttctc tagaggtgaa agctggagaa acaattgctt
    21781 ttgttgggcc atctggctct ggaaaatcta cactccttaa cttattgatt ggatttatcc
    21841 agccgacttc aggaaaaatt tttctagatg atcagccttt ggatgaactg gatatgcgaa
    21901 gtgtgcgaaa ttatttagcc gttgttcctc aaaccacttt gttgttctcc gcttcgatca
    21961 aagagaacat cacttatggc ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg
    22021 aagctgcgca actttcttca ctgattgacc agcttcctga tggtctggat accttagtag
    22081 gcgaacatgg caacaagctg tctggtggcc aaaagcaacg tatctccatt gcacgagcgt
    22141 tgatacgtca gccaaaaatc attttgctag atgaagcaac ctcagcttta gataaccagt
    22201 cagagaaaaa aatccagcaa gccctgaatt acctgacaga aacacccact acttttattg
    22261 tggctcatcg gctttcaacg atcaaagagg cagataaaat cgttgtgatc gatgaaggaa
    22321 aaatcgttga aataggtact tacgaaaaac tgttagctca aaaaggctat ttctaccgct
    22381 tgaaaaatga atgacaaaaa ataggagcta tgacaagact tttgtcgtag ctcctatttc
    22441 tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt catcaaacat
    22501 gagaagctgt tttttgaaat tgttccttgt aactcagagt taaacggtag gctcacgacc
    22561 tcttggtaaa tggtgttcaa gagacagcct atcggcaata agaagcaatt ttgtcatcgc
    22621 tcaaggccga acgtctcttt cacaccctct gttgtttcat aaaatagttt tattcgctgt
    22681 aaaggtcgcg aactggtgtc atgatgatac ggttcaagtc attgatgatt gtactgaagc
    22741 cttgctcttt ttgcatcaag tcattgatta cttcttctga ttggattttc acagccattt
    22801 cttgtgcttt cattgcatct tcgtcgctga actcttgtcc actcatttgt ttttcttgta
    22861 attcgatttg catcgcttgg aagtctttga acagtttgtg tgcttcttcg tttgcttgta
    22921 ctttctcgta cgcttctttc aacgctttga attctggtaa ttcacggatc tcacgttcga
    22981 tttgattcgc gctatcgtag atgtttgaca tgttatgccc tcttttcttt tagtagttac
    23041 tctctattgt aacatggggt tattcgtttt caaaggggct actctccttg atatttccgc
    23101 cagaggtctt tagccgcttc accaattccc tgaacgcctt ctttcaccat atcgccgaag
    23161 tttttcagcc cttcttttgc ttgttcacca tattgattca agttatcttg ccaatttcca
    23221 gactctccat tagaatttcc tgtgtttcca acttgatctg cagctaccac ttgtcctcct
    23281 gtggcatagg catctgctac actaaatcct gtttttgaag tatacggaag gatgccttct
    23341 gccgctgatt tgaagacttt cccgacaaca tttccacttg tcccttccaa atagtgcagc
    23401 ttgctggatt cttcgtaacc catccatgtc gaaatcacta cgtctggcgt gtaaccgatc
    23461 atccattggt catttgcttt tgtggcatcg aaattagttt ctgtggttcc tgttttccct
    23521 gctatcgtat aacctgcagg attcgcagca gccgctgttc catttgaaaa tgtaccaaga
    23581 agcatgctgg tcatttcatc ggctgtttcc tttgatatga cctgttttgg ttttttgttt
    23641 gtgttatcga cgatcaccgc tccagtcgaa tcaacgattt tcgtgataag atgcggcgta
    23701 tacatctgac catcattggc aaatgcactg taagcacccg ccatgatcaa tggagatact
    23761 cctttttcca gtccaccaag ggctaagcca taatacttat cagattttgc taaaggcagg
    23821 ccgaattctt ctgctttatc atatcctttt tgtaaaccga tctcatgcaa aagccataca
    23881 gtaggcaaat tcaaactttg tgcaacagct tgatacatcg gtacttctcc ttggtatgtc
    23941 ccgtcaaaat tatgcgcatc gtaataactt tgcggttcgt ctttcaagat actggaaggc
    24001 ttgtatccag cttccagtgc tggtgtatat acgcttaatg gtttgatcgt tgatcctggt
    24061 gaacgacgca tctgtgtagc aaagctgaat cctctaaaga catgatctcc tcttcgtcct
    24121 actagtgctt gtacaccacc tgtattcgga tctaaagcta ctgaagcaga ttggaccatg
    24181 gcaccatctg aagcatttgc tgggaataat gcactgtttt gataagtagc ttctaattgc
    24241 ttttgataat cctgatctaa agctgtatag attttatagc ctttattcat gacatcttct
    24301 tcatcaagac catactcgtt aactgcttca tcgatcactg catcaaaata ataaggataa
    24361 cgataaccag aatctgaatt atcgtatgtg tcattaagat acgcgctgat atctgtctgg
    24421 ctttctgctt gttcttgttc ttttgtgatt tttccgtttt cttccattac acttagtact
    24481 gtgtttttcc gatcattcgc attttttggg tgatcgatcg gattataaat accaggacct
    24541 ttcagcattc ctgccaaagt agcagcctca gacagcgtaa cattcgttgc atctactcca
    24601 aaatactttc tggaagcgtc ttggacaccc cataccccgt tgccaaaata ggcattgtta
    24661 agatacatgg tcaagatttc ttctttgctg tacttctttt cgatctcgat tgccaaaaat
    24721 aattccttcg cttttcgatc aaacgtttgg tctaatgtca gatacgcatt tttggcaagc
    24781 tgctgagtga tcgtacttcc tccgccccct gatgttccgc cagacaagac cattcggact
    24841 gctgcacggg caatcccttt gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct
    24901 gtcgagatta ctgcatcttg gatataggga gaaatcgcgt ttaattctac atatgttcct
    24961 ttttgggcgt acagtgtgcc agcttcttcg tttttcttat cataaatgac tgtcgattgg
    25021 cttaatcctg attttaacgt ttcgacattt gcttgttttg ctaaaataaa gaggtagata
    25081 cttgtcacta agacacagat caaaccaatc agcaaaatca atttgttcac atgatatttt
    25141 ttccagatgc gctttcgcca tcgatggaat tgtccgagat atggtctgat ccaacgccaa
    25201 aaactgatca aagcagcttt tatcttttct aaaatgcttt gaaaatccat ttgcttcttc
    25261 tcctgtctat ttgattcaat cgtcttattt taccatagtg aaagagtgtt ttcttcctgc
    25321 ttaagttgta gttcaatatg tttttttacg ttagtgcaag tagagtaaca taagaatgaa
    25381 cagaaaaatg taagtttttt cttaaatatg ttttaaaaat ggtacaatta gtctgcgatt
    25441 gcactgaaag gagtacactt gtgaaaatca cgatcacttt acctatgaac catcctgaaa
    25501 caacaattcg cgacttgctt gaaaaggaat ggttagttcc tagaaaggtt cgacactttt
    25561 tgcgtatacg taaaaacgtt tggatcaatc aacaacctgc tttgttccat catgaagtaa
    25621 agggcaatga ccagatcaca ttgttattcg aagaaacaga ttataattat caagaagttc
    25681 aattaggaga tgcgaagcac atccgtgttt tatatgaaga tgagcatttg attatagtgg
    25741 ataagcctgc aggaatgaag actcatccaa atgaaccgac agaaaacagt accttactca
    25801 atcatttagc tgcttatctc gacactaaac agcaacgacc ttacgttgtc caccgcttgg
    25861 acaaagaaac aagcggtgcc gttttatttg caaaaaatcc atttgtcttg ccgatacttg
    25921 gaagaatgtt ggaacaaaaa atgatctatc ggagatatca agccatcgta tggggagaaa
    25981 ttgccaaaga tatgaccatc accaataaaa ttggacgaga tcgccatgat cggagaaaaa
    26041 gggtcgtaga tgataaaaat ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac
    26101 tttctgaaca aacacaagtc tattgtgttt tggaaaccgg acgtacacac cagatcagag
    26161 ttcatttagc tcataaggga catccgatcg ttggagaccc tctctaccaa aatagaacag
    26221 ctgaacgact gatgctccat gcattagaac tgcacatgat ccatccattt actcaagaaa
    26281 aaatagtcgg taaagcttta cctggattat gggaatagaa ataaaaaaga acagagtctt
    26341 atcagcagat aatgataaga ctctgttctt tttgtgtaat ttgtctgctt acttttgatg
    26401 ttcgtgttct gctaacagca aactaccgat aatcccactt tcattcggca gtccccaacg
    26461 aacgatatat tcacttactt gaggtgtttc catataacca cccatcaatt caacaaattg
    26521 ctgacggatt ttttgaagga gatgatcctg attcattacc ccaccgccta aaataatctt
    26581 ttcaggtgct aacgtcaacg tatagttgat cagtgcttga gcaatataat aagcctgaat
    26641 agcccaaaca ggatgatctt gcggcagatc ttgccctttg atccctgtgc gtgcctcaat
    26701 agacggtcct gctgctaatc cttctaaaca atctttatga taaggacaag taccttcata
    26761 atggtcatct ggatggcgtt tcacccaaat atgtcccatt tcaggatgtg cgatccctga
    26821 aaaaatttct tcattgatca cgacacctgc accgattcct gttcccacgg taaggtaaat
    26881 acaactattt tttccttgtg ctgctccttg cttcaattcc ccatatgcag cagcattgac
    26941 atccgttgtc caagcagcag gaatattgta gcgttccttg attgtgccaa gaaaatcgaa
    27001 ttgtgcccac ccttttttgg gagttgccaa aacgtatccg taattatctt ttgttttgtc
    27061 gataccaatc gggccgaatg agccaatccc gattcccttc aattcataac gatcaaaaaa
    27121 ttcaaatacc tgatccaatg tttcttctgg tgttgtggta gggatactta attt
//
LOCUS       DAZKNM010000017        26555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000017 DAZKNM010000000
VERSION     DAZKNM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.017"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG8M9_000620"
     CDS             complement(104..1336)
                     /locus_tag="ACG8M9_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0377414.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG8M9_000621"
     CDS             1591..2160
                     /locus_tag="ACG8M9_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0377415.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG8M9_000622"
     CDS             complement(2338..2778)
                     /locus_tag="ACG8M9_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0377416.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG8M9_000623"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG8M9_000623"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0377417.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG8M9_000624"
     CDS             3732..4655
                     /locus_tag="ACG8M9_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0377418.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG8M9_000625"
     CDS             4731..5870
                     /locus_tag="ACG8M9_000625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0377419.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG8M9_000626"
     CDS             5863..6663
                     /locus_tag="ACG8M9_000626"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0377420.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG8M9_000627"
     CDS             6663..7490
                     /locus_tag="ACG8M9_000627"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0377421.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG8M9_000628"
     CDS             7495..8202
                     /locus_tag="ACG8M9_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0377422.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8M9_000629"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8M9_000629"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0377423.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8M9_000630"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8M9_000630"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0377424.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8M9_000631"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8M9_000631"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0377425.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8M9_000632"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8M9_000632"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0377426.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG8M9_000633"
     CDS             11787..13820
                     /locus_tag="ACG8M9_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377427.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG8M9_000634"
     CDS             13864..15144
                     /locus_tag="ACG8M9_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0377428.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG8M9_000635"
     CDS             15354..16160
                     /locus_tag="ACG8M9_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377429.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG8M9_000636"
     CDS             16172..17392
                     /locus_tag="ACG8M9_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377430.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG8M9_000637"
     CDS             17382..18968
                     /locus_tag="ACG8M9_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0377431.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG8M9_000638"
     CDS             19007..21145
                     /locus_tag="ACG8M9_000638"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0377432.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG8M9_000639"
     CDS             complement(21513..22496)
                     /locus_tag="ACG8M9_000639"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0377433.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG8M9_000640"
     CDS             22874..24265
                     /locus_tag="ACG8M9_000640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0377434.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG8M9_000641"
     CDS             24281..25321
                     /locus_tag="ACG8M9_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0377435.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="ACG8M9_000642"
     CDS             25340..26425
                     /locus_tag="ACG8M9_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0377436.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26555
                     /locus_tag="ACG8M9_000643"
     CDS             26458..>26555
                     /locus_tag="ACG8M9_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFQ0377437.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIH"
BASE COUNT         8496 a         4431 c         5404 g         8224 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catat
//
LOCUS       DAZKNM010000018        24835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000018 DAZKNM010000000
VERSION     DAZKNM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.018"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACG8M9_000644"
     CDS             complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACG8M9_000644"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0377438.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(2013..2390)
                     /locus_tag="ACG8M9_000645"
     CDS             complement(2013..2390)
                     /locus_tag="ACG8M9_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377439.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            2646..3374
                     /gene="purC"
                     /locus_tag="ACG8M9_000646"
     CDS             2646..3374
                     /gene="purC"
                     /locus_tag="ACG8M9_000646"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0377440.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            3374..3628
                     /gene="purS"
                     /locus_tag="ACG8M9_000647"
     CDS             3374..3628
                     /gene="purS"
                     /locus_tag="ACG8M9_000647"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0377441.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            3630..4301
                     /gene="purQ"
                     /locus_tag="ACG8M9_000648"
     CDS             3630..4301
                     /gene="purQ"
                     /locus_tag="ACG8M9_000648"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0377442.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            4302..6524
                     /gene="purL"
                     /locus_tag="ACG8M9_000649"
     CDS             4302..6524
                     /gene="purL"
                     /locus_tag="ACG8M9_000649"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0377443.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            6509..7948
                     /gene="purF"
                     /locus_tag="ACG8M9_000650"
     CDS             6509..7948
                     /gene="purF"
                     /locus_tag="ACG8M9_000650"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0377444.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            7971..9023
                     /gene="purM"
                     /locus_tag="ACG8M9_000651"
     CDS             7971..9023
                     /gene="purM"
                     /locus_tag="ACG8M9_000651"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0377445.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            9020..9598
                     /gene="purN"
                     /locus_tag="ACG8M9_000652"
     CDS             9020..9598
                     /gene="purN"
                     /locus_tag="ACG8M9_000652"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0377446.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            9595..11130
                     /gene="purH"
                     /locus_tag="ACG8M9_000653"
     CDS             9595..11130
                     /gene="purH"
                     /locus_tag="ACG8M9_000653"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0377447.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            11146..12420
                     /gene="purD"
                     /locus_tag="ACG8M9_000654"
     CDS             11146..12420
                     /gene="purD"
                     /locus_tag="ACG8M9_000654"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0377448.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            12542..13456
                     /locus_tag="ACG8M9_000655"
     CDS             12542..13456
                     /locus_tag="ACG8M9_000655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0377449.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(13589..15154)
                     /locus_tag="ACG8M9_000656"
     CDS             complement(13589..15154)
                     /locus_tag="ACG8M9_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377450.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(15147..16598)
                     /locus_tag="ACG8M9_000657"
     CDS             complement(15147..16598)
                     /locus_tag="ACG8M9_000657"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0377451.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(16612..17628)
                     /locus_tag="ACG8M9_000658"
     CDS             complement(16612..17628)
                     /locus_tag="ACG8M9_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0377452.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            17848..18936
                     /gene="chvE"
                     /locus_tag="ACG8M9_000659"
     CDS             17848..18936
                     /gene="chvE"
                     /locus_tag="ACG8M9_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0377453.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            18990..20516
                     /gene="mmsA"
                     /locus_tag="ACG8M9_000660"
     CDS             18990..20516
                     /gene="mmsA"
                     /locus_tag="ACG8M9_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0377454.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            20518..21723
                     /gene="mmsB"
                     /locus_tag="ACG8M9_000661"
     CDS             20518..21723
                     /gene="mmsB"
                     /locus_tag="ACG8M9_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0377455.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            21980..22957
                     /gene="guaC"
                     /locus_tag="ACG8M9_000662"
     CDS             21980..22957
                     /gene="guaC"
                     /locus_tag="ACG8M9_000662"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0377456.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            23221..24645
                     /locus_tag="ACG8M9_000663"
     CDS             23221..24645
                     /locus_tag="ACG8M9_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0377457.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         7836 a         4317 c         5529 g         7153 t
ORIGIN      
        1 aactgacctt ccgcccctct cacaccaccg tacgtacgga cccgtatacg gcggttcaat
       61 aagttgagtg tacgaactgg tacttcaagg agatgtcctc atatccttgg cgggccagtt
      121 ctttgtctgt taatgatcga tgaagaatat gacttttgga gactgaccaa ataccttttc
      181 ttgtatttga ccattcatac gctttcgctt tatcgatccc taaacgaatc agattttttc
      241 ttttggttct tggattcttc cattgtttcc aaatatattg tctgattctt cgcttcaacc
      301 aaccgtttaa ctccctcatg aattctttca tctcaccaat tccatagtag ttgatccaac
      361 cagtcattag ttgcttgatt tcttttaaaa taagttggat acttcttcca cggtttcttt
      421 tagtgagttg tttgagcttg cttttcacac gctgttttgc ttttgcgtga gggcgaattt
      481 taataccttt ctttgttggc agaatgcaga atccaagaaa ctttcgtttc aatggacttc
      541 ctaccgcact cttttctcga tttaccgtca actttagttg ttcttcgaga aattgaatgg
      601 atttcttcat cacacgctgt cctgctcgtt tgcttctaac atagatattg cagtcatcgg
      661 cgtatcgaac aaatttatgt cctagatttt ctagcacttg atcaaattcg ttcaggtaga
      721 tgttacttaa caggggagag agattgcctc cttggggcgt tccttcttca gttgttgtga
      781 tgagtccatt ttccattaca ccactcatta agtattgacg gatcagtttt aggacttgtt
      841 tgtcctttac tttccgctca atgaaataca tcagcttatc gtgatttacg gtgtcaaagt
      901 aggctttcat atcaatgtcc accacatatc ggtaaccttg ttcataataa gattttgctt
      961 tcttcactgc gtcatgtgcg ctgcgatttg ggcgaaaccc ataactatta tctgagaatt
     1021 ctggttcaaa tagcggttgt aacacttgat tgattgcctg ttgaagcata cgatcaataa
     1081 ccgtgggaat tcctagtttt cttacaccgc cgtttggttt cggtatttct actcgcaaaa
     1141 caggctgagg tttatagttc ccttggcgta aattggatag taattcctcg cggtgctctt
     1201 tcaagtaagg aagcagttga tcgacggtca tcccatctgc accagccgcc cctttatttc
     1261 tgactacttg aagatacgct tgattgagat tattccgatc tagaattctc ccaaacagat
     1321 tttctacacc atcttgtttc ctctgttcac cgcaagaagt gctgcgcact tcatcatacc
     1381 cttcgtgttc cacactagct ctttgatgat agccaagttt tcttgttttc tgcggtcgtc
     1441 gcatcttctc acacctccta aattattcaa gattcttatt gttcagtcct tcgtaacggt
     1501 cagtgtcgtt actactatga cctcggctga cttctcatga ttcaccatca tgtcgccatg
     1561 atcgttgtgc tggtttgttt tcattcaacg cccacaacac cctcatgaga tctcccccgg
     1621 taagagtgaa aactttcctc ccatgtaact gctagattta ctgtataggt ttcgggcagt
     1681 attggacttt gtcttgtgat gaagacttat ccgacctaat tcagccttgt atctagtttc
     1741 tgttcgtcag ttcaggagtt tgcgtccgac ttccttcagc cacaacctca cgattgccgc
     1801 cttgtctttc gctaacagtt cctactgcca agcctgtagt ggactttcac caccaagttt
     1861 tcacccatgc agggcgcaca ccaaaaaaga agcagatccg ttagcggttc tgcttctttt
     1921 agtttattat attttcttat caataatagg aacctgattt taaaagcggc tacctcttta
     1981 ccgtgcctct ttacatacaa aaaatagagg aattaagcgt aaatggcttg gctattataa
     2041 ctaaaatgct ggttatggag atgttgcagc aagttttcga gaaaagcgta ttccatctct
     2101 tgtccattga ataagatctg gatacgataa tgatttttga aacgcttctg ttcatgaatc
     2161 tgactgattg aaagttcgcc tttgtgttct gtctgataaa gaaaagtcct tttacggcat
     2221 tcagcacagc gcttggttgc tcggcggatt tttcggaatg tataaaaact agtatcttta
     2281 ttgcataaat aaacgtcttt gatcgacagc aaatcattat atagacagga acgttcttca
     2341 gctgttaatt ctgataaaaa aagctgacct tgttttattg catcttccat cggcaagtgc
     2401 ctcctcgaac ccattatcca aatttttagt taattctaat ataccatcat taagtataaa
     2461 tgtaaacgct tgttgatggt ataagatttt cgtttctgat aaaacacgaa cattaaaaga
     2521 atctaatgcc ttaaatgtac atatttttgt tgatttataa aaagatgatt gctaaaattt
     2581 aaagtgaaaa aacgaacaaa aaacgatgta tttgctttta atgtaaataa aaaggagaga
     2641 cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc caaaaaaata tatcttactt
     2701 ctgatgagaa cttgatttgg atagaatatt tagatcaagc aactgcgttg aatggcgcga
     2761 ggaaagacca gataagtgga aaaggagaat tgaataatca aatcacaagt gcaattttcc
     2821 gttatctgac tcaaagagga ataaaaaatc attttattga acaattgtct aaaaccgaac
     2881 aattagcaaa aaaagtacaa attatccctc tggaagtggt tatccgaaat attgcagcag
     2941 gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg
     3001 aattctacta taaggatgac cagttggatg atccatttat caatgaagag catatctatt
     3061 tcttacaact tgcaaagccg gaagaaatca gtgagctgaa agcccaagcg ttggctatta
     3121 atgaaatttt gaccgaatta tttgctaagg tcggtttggt tttagtagat ttcaagctag
     3181 aatttggccg gtcgaatgga caaattttgc ttgcggatga agtaacaccg gatacttgcc
     3241 gattatggga tgaacgtacg aatgaacata tggacaaaga cgtttaccgc agagatctag
     3301 gtgatttgat cccagtctat caagaagtat ataaccgtct atcggaacta actgaagaaa
     3361 aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc tataaagatt cagtgttaga
     3421 tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga
     3481 tatccgaata ggaaaatact ttgagataca acttgctgat gcagaagaac caatcgatac
     3541 agtgattgaa acgatttgtg acaagctgct tgcaaatgta aatatggaaa cataccgata
     3601 tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt
     3661 caaattgtga cgcagatatg ttatgggcag ttcgcgagat catgggcgct gatgccgaat
     3721 ttgtacggca tgatgagtcc tctttagaag gatttgatgg ggttttactg ccgggaggat
     3781 tctcctatgg tgactatttg agatgcggtg cgattgctcg gttttctaca atcatggaag
     3841 aagtgattcg ttttgctgaa gaagggaaac cagtattcgg tacatgcaat ggattccaga
     3901 tattgacaga agcaggactt ttgccaggtg tactgagacg aaatgagtct ttgcatttta
     3961 tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc ctttacttca gagtatgaag
     4021 ccgacgaaat aatctatctt ccagttgctc atggggaagg aaattatttc tgtgacgaag
     4081 aaacacttca agaactaaaa aaaaataatc agattgcctt tacttataat gacaacatca
     4141 acggcagcgt ggaaaacatt gcagggatta cgaacaaagc agggaatgtg ttaggaatga
     4201 tgcctcatcc agaacgtgca gtagaagact tgctaggatc agaagatggg aaacggttct
     4261 ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac
     4321 cgacagctga agagatcaag aaaaaaagaa tctatgccga atggggattg actgatgaag
     4381 aatatcgaat gatcgaagaa gatatcttag gaagaatgcc taattatacg gaaactggac
     4441 tgttttctgt tatgtggagc gaacattgtt cctataaaaa ttctaaaccg gttctacgca
     4501 aatttcctac tagtggacct catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg
     4561 acatcggtga tggactagca gttgtcttca aagcagaaag tcacaatcat ccttcagccg
     4621 tggagcctta tgaaggagct gcgacaggag ttggcggaat catcagagat attttcagca
     4681 tgggggctcg tccaattgcg ttgctagatt cattgaggtt cggcgaatta gataatccaa
     4741 gaacgaaata tttactagaa gaagtagttg caggtatcgg cggatacggc aattgtatcg
     4801 gcataccgac tgtaggcggc gaaattgcat ttgatccttg ttatgaagga aacccattag
     4861 tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat ccagaaaggg caagccgctg
     4921 gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg tcgcgacggt atccacggag
     4981 caacttttgc ctcagaagaa tttaatcaag aagaagaaca gcaacgttct gctgtacaag
     5041 taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg tttggaatta atcttgcatc
     5101 attcggattc tctaataggt atacaggata tgggagcagc aggacttgtt tcatctagtg
     5161 cggaaatggc ttcaaaagct ggctcaggac ttatattgac attagatgaa gtcccacaaa
     5221 gagaaactgg gatgacacct tatgaaatga tgttatcgga atcacaagaa cggatgttga
     5281 tttgtgttaa atcaggagag gaagaaaaaa tccaagaact atttcagaag tatgatctag
     5341 atgcagtcac tatcggtaaa gtaacagacg acgggcaata tcggctatat catcaaggag
     5401 aagaagtagc aaatctgcca gtcgatgcat tagctgaaga tgcaccagtt tatcataaag
     5461 aaatgaaaga accagctcga atcgtagaat ttcagcagtt agctccttat cagccagtta
     5521 tcgaagaacc tggacaagta ttgcttgatc tgctgcagca accgacgatt gcttcaaaac
     5581 gttcaatcta cgaaacttat gattcacaag tacaaaccaa tacagttgtc cgtccaggaa
     5641 gcgatgctgc tgtgatgagg gttagaggga cgaataaagc gttagctatg accacagact
     5701 gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg acaaatagca gtggcagaag
     5761 ctgctagaaa tattattgct agcggtggta agcccttggc gatcaccgac tgcctgaact
     5821 atggttctcc tgataaacca gaagtttttt gggaattatc gacttcagct gacggaattg
     5881 ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg aaatgtctct ttatataatg
     5941 aaacggatgg tcaagcaatc tatccaacac caatgattgg tatggtcgga ttgattgagg
     6001 atcataaaca tatcactact caggaattca aaaaatcagg ggatctaatc tatatattag
     6061 gtaagacgtt tgcagatttt gatggcagtg aattacaaaa gatgcagctt ggaagaatcg
     6121 aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag aaaccaagag ttagtattga
     6181 cagccatcca aaacggattg attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa
     6241 ttgcactggc agaatcagct ttcaaacatc agctagggat atctgtacag tttgaattgt
     6301 cctctgctca attatttgca gaaactcaat caagatttgt gttgacagtc gcaccggaaa
     6361 ataagacacg attcgaagaa atgatgggag atgctgctgt tttagcaggt aaggtgacgg
     6421 atgaagcgat aatcgaaatt tcagcaactg acggtcagat caaaatagaa actgcagtag
     6481 caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga
     6541 atgcgggatc ttcggtgttt ggggtcatcc agacgccgct agtgtggcat acttcgggct
     6601 ccatagtctt cagcatcgag gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa
     6661 gcttaagggg catcgagatt tggggctgct ttctgaggta ttcaaagatc aaagaaaatt
     6721 gcagcagtta acaggagaag ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg
     6781 aagtgtggac aatattcagc cttttctatt caaatttcat gatcagtcga tcggcttagc
     6841 gcataatggt aacttgatca atgctcgttc ccttagaaaa gagttagagg aaaatggggc
     6901 gatcttccat tctaactccg atacggagat attgatgcat ttgatccgac gaagtgaaga
     6961 aacagatttt ttaaataaat taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta
     7021 tttattaatg acagaaacgg caatgattgc cgctttggac ccaaatggtt ttcgtccatt
     7081 agcaatcgga caaatgaaaa acggagcata cgtgattgcc tcggaaactt gtgcattaga
     7141 agttattggg gcacgtttta taagagatgt ccagcctggt gagatagtga tcatcaatga
     7201 cgaaggaatc cagattgatt gctttacgaa ggacacgcag ctttctattt gttcgatgga
     7261 atatatctat tttgccagac cagattcaaa catcgcaggg atcaatgtgc atactgcacg
     7321 gaaaaatatg ggacggaatt tagcgaaaga atcgccagta aaagcagata tggtcgttgg
     7381 cgtacctaat tcttctttgt cagccgcaag cggttatgcg gaagctagtg gaatccctta
     7441 tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca tttatccagc caacgcctga
     7501 gctaagagag caaggtgttc gaatgaaatt atctgctgta cgaggagtag tggaaggtaa
     7561 aaaggtgatt atggtcgatg attcaattgt tcggggtacg acaagcagac ggatcgtcca
     7621 tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg attgcttctc ctcctttgaa
     7681 atatccatgt ttttacggga ttgatattca aacgcgtaaa gaactcattg cggcaaatca
     7741 cacgattgat gaaatcaaag aatgtatcgg tgcagattct ttaggttttt taagtgaaga
     7801 gggtctgatc gaatcgatcg gtctggaaaa agatgctccg tattcaggat tgtgtatggc
     7861 ttatttcaac ggagattatc cgacgccgtt atatgactat gaagaaaaat atcaggaatc
     7921 actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag aagggagtat atgaattcaa
     7981 tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc tggatatgaa gtagtagaac
     8041 ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat gatgggcgct ataggaggat
     8101 tcggcggttg ttttgattta tcggtctttc aattaaaaga gccagtcctt gtctcgggga
     8161 ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc agacaaacac gatacaatcg
     8221 ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc acaaggcgca gagccgttat
     8281 atttcttaga ctacttagca acggggaaaa atgatccgtt acgtttggaa aatgtagtaa
     8341 aaggtgtagc agatggatgt gttcaaggaa atatggcgct gattggcgga gaaacagctg
     8401 aaatgcctgg tatgtactca ggtaatgatt atgatctagc cggatttgct gtagggatag
     8461 ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa
     8521 tagcatctag cgggattcat tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc
     8581 agcagcagtt ttctttatca tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat
     8641 tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa
     8701 taaaaggagc tgcacatatc actggtggag ggtttgtaga aaatatcccg cgaatgttac
     8761 cagaatctat ggctgcacaa atcaaacttg gctcatggcc gattcttcca atattcggct
     8821 gtttacaaaa atatggtgaa attcctgaga tggagatgta tgaaatcttc aatatgggca
     8881 tcggtatggt cctggcagtc caagaatcga aagtaaaaga aatagtacac gaaattgagc
     8941 aagctggcga aaaagtttat gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg
     9001 tgtttatagg agaagaacga tgagaatcgc tgtttttgct tctggaaacg gaagcaactt
     9061 tcaagcgcta gccgattatc tatcgaaaaa aggattggaa tcttcgattg actggttgtt
     9121 ttgtgatcag cctgaagcat atgttttgaa acgtgcaact gcgttgagtg taccggcaga
     9181 ttgtttttca cctaaagaat ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa
     9241 attgaaagaa aaaaagatcg atttgatcgt attagctgga tatatgcgaa tcattggacc
     9301 ggtattgctg aaaaattatg ataaaagaat catcaatatc catccgtctc ttttacctgc
     9361 cttccctggt ttgcatggca tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt
     9421 cacaattcat tatatcgacc aaggtgtaga tactggacca atcatccggc aggaaaaagt
     9481 gagaattgaa caggaggaca cgttcgattc tttagaagaa aaaatccata gagtagagca
     9541 tcgaatctat ccagaagtga ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca
     9601 agagcattga tcagtgtatc agataaaact gggattgtag cattcgccca aggtttgatt
     9661 gaacaaggag tagaaatcat ttcaacagga ggaacaaagc atgcgttgga taaagctggg
     9721 atcaaaacgc tttcgatcga agaagtgaca gggtttcctg aaatgatgga tggccgtgtc
     9781 aaaacacttc atcctaaaat ccatggtggt cttttaggaa gacgcgatct ccctagccat
     9841 atggaggcaa tggaaacgga aaatatccaa ccaatcgact ttgtttgtgt caatttatat
     9901 cctttcaaag aaacgattat gaaaccagat gtcgaaatag cagaagctat tgaaaacatt
     9961 gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa accatgaatt tgtcacctct
    10021 gttgttgatc catctgacta tgaagaagta ctttcagaat tacgtcagta tgggcatact
    10081 tcactttctt tcagacaaaa actagcggca aaagtctttc gtcatactgc ggcatatgat
    10141 gcactgatcg ctcaatactt aacggattgg gtggaagaaa aagaaccgga aaaattgaca
    10201 cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa atagccatca agccgctgcc
    10261 ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag cagcaaaaca actccatggg
    10321 aaagaacttt cttataataa tatccgagat gcagatgctg cgctacggat catgaaggaa
    10381 tatgacgagc caacggtagt cgccttgaaa cacatgaatc cttgtggtat tgggacagca
    10441 tctacgattt tagaagcgtg ggaagcagcg tatgaagcag atcctgtttc aatctttggt
    10501 gggatcatcg ttttgaatcg agaagtagat ctgccaacag cagaacagat gcataagctg
    10561 tttctagaaa tcatcattgc cccttcttat acacaagaag ctttggaatt actgcaaaaa
    10621 aagaaaaaca tccgattact gacagtagac tttgcgcagg aagaaaatca aaatcgagaa
    10681 gaaacggtct cagtcttagg tggacttctt gtacaagatc aagatctggc aatagaaaat
    10741 gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc cagcagaagc aaaagcatta
    10801 gcgtttgctt ggagagctgt caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa
    10861 cgtcagacag taggaatcgg agccgggcag atgaatcgag tcggttcagt aaaaattgcg
    10921 attgaacaag cgcaagagaa aatggaaggt gcagttttag ctagcgatgc ttattttcca
    10981 atggatgatt cggttgaata tgcagccaaa catggtataa aagcaatcat tcagccagga
    11041 ggaagcatca aagaccaaga ttctattgat atggcgaata aatacgatct agcaatggtc
    11101 tttactgatg ttcggcattt tagacattag agaggagtcg gataaatgaa cattttggtg
    11161 atcggtgcag gtggtcgtga acatgcaata agtaaaaaac ttttagccag tccgaaggtg
    11221 gatacggtgt attgtgcgcc aggaaatccg gggatgacaa aagatggtat caattgtgtc
    11281 gctatcagtg agcgagatca ttcagcgtta atccattttg ttaagaaaga ggctattgat
    11341 tggagtatcg ttggtcctga agttcctttg ttaaatggga tcgtcgatga ttttatggcg
    11401 gaaggattga aaatcttcgg tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt
    11461 tttgctaaac aattaatgga tacttatcaa atcccgacag ctaaatccag aagttttgaa
    11521 tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg
    11581 gacggcttag cagctgggaa aggtgtagtc gtggctacca cagtgaagga agcgctggat
    11641 gcggcagaac aaatgttgaa ccagcatcga tttggggata gtagcaaaaa aatcgtcgta
    11701 gaagagtttt tggcaggaga agaattctcc cttttggcgt ttgtccgtaa taccaaggtg
    11761 tatccgatgg tcatttcgca agatcataag cgggcatttg ataacgataa agggccaaat
    11821 acaggcggaa tgggtgctta ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa
    11881 gctgtggaag aagttttaaa accagcggct ttgggaatga aagaactagg acgctctttc
    11941 acgggtgttt tatatgcggg tttgattgct acgaaggaag gaccaaaagt gatcgaattc
    12001 aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt
    12061 gcgcaagtaa tcgatgattt attagagaat cgtacagtag agttaaagtg gcagcaagaa
    12121 ggatacaatg taggcgttgt ggttgcagct gccggttacc ccgaagaata tgagacagga
    12181 atggttctac cagattttct ggaagaagag ctttctgttt actatgctgg agtttcctca
    12241 aataacggca aactagttgg agcaggagga cgtctttatt tagtagaagc atacggtgag
    12301 acgatcaaag aagcacagaa aaaaatttac accgcgttaa tggataaaac gacagatgga
    12361 acattttatc gtacagatat tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa
    12421 atcagctgct tccatttata aaaaatacaa caaacataat cggaagcgcc gattttagtg
    12481 aagccttttc ataaagagtg tggtacactt ttgttgtgta tataaataag gaggagttca
    12541 gatgaaaaaa attcaattag gtacttctga tcagaaagca gctgcagtga tcttaggctg
    12601 tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt gagacagctt atgaaaacgg
    12661 cattgatttt tttgaccatg cagatattta tggaaacggt gaatgtgaag aaatctttgc
    12721 agatgctcta gcaaaaacat caatcaaacg agaagatctt tttattcaaa caaaatgtgg
    12781 aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat atcattgaat ctgtagaaaa
    12841 aagtttgacc cgtctgaaaa tggattatgt ggatgcttta cttcttcatc gtccagatac
    12901 attggtagaa ccagaggaag tagctgaagc gtttgatgaa ttggaacggt caggaaaagt
    12961 tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt
    13021 tcgtcaacca ttagtagcta atcagctgca atttggcttg aaacatactg gaatgatcga
    13081 tcaagggatt catgtcaata tgacagatga agcaagtatc gatcatgacg gtagtgtact
    13141 tgattattcc cgattgcatg atatgacgat ccaagcatgg tcaccatacc agtatggatt
    13201 ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga
    13261 actagcagct aaatatcaaa cgacaccaac tggcttggct tctgcttgga ttttacgcca
    13321 tccagccaat atgcaagtga tcgccggtac gatgaacgta gggcgaatca aggaaatcgc
    13381 tcaagcttca gaaattaata tgtctcggga agattggtac gaattgtacc gtgctgctgg
    13441 aaacatttta ccttaaagat tgtcataagc aggtcaaaag ggctataaat taaaaagaca
    13501 gaataacttg aaagctgttt tcagcttcga gatattctgt ctttttttat tgcaaatgga
    13561 aagagtatca ttgtacggat ttcgtttatt agttattttt gtttttaagg aaaagctgtg
    13621 tccgatattc ttttggagaa atcccttgtt ttttcttaaa taaataactg aaatagttag
    13681 ggtcattata accaatttca aaagcaatat ctgaaatacg atagtctgat tgctgcaata
    13741 atttttttgc taattcgatt ctttgttctg tcagataatc gatgaatgtt tggttaaacg
    13801 attgagaaaa aatcgtactg aaatgagagg gacttaatgc aacttgttga gcgacccaag
    13861 ttaaggacat atctggatcg gtaaaatttt ctttgataaa atccaatgca tggttcaaaa
    13921 ttggctgata tttgttcctt gaaggctgtg agggcagagc agttaagaaa gaaatgaaca
    13981 tataaactgt cgaacggtaa agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg
    14041 atgtgcaatg aaccatttct tgcgggattt tcttttcttt caggcagaca tgctggagta
    14101 gcttagtcaa gacaaataaa cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg
    14161 tcaagtgttc gatgtactta gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga
    14221 ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc
    14281 tgataatttg ttctttaagg gaactggcgg tttcaagtat catctgcgtg atctcaaaag
    14341 atgcaggtaa ttcgctgatt ctttctgcga caggaccaat ggcgacagaa aaattatttt
    14401 tttgtccctt ttctaattca tggaccaacg tttgggctac ctgataactg gtctcgatca
    14461 cattttcttc aagagaatca actattaaaa atttgatgaa tcgagaggaa acagcagaga
    14521 agatcacttg ttgattctcc ctaaataaaa acttcaaata atcactgaga tgagcgtaat
    14581 cttcaaagtc tgtactgact tggtttgctg ctaataatac ggtcattttt ttccctgcta
    14641 aagagcgatt gaaagattca gcaagtttca atgtttcact gatcgtaagt gagcctttga
    14701 acaaaccgtt caagaaatga ttttttttca attcgaaaac aagatcagaa gaagtgtgcg
    14761 tttctttttg ctgcaaatct aaagcagtta ctgctttttc tattgcttgt ttcaattcgc
    14821 tacttttaat cggtttaagc agataatcat ctgcctgtac ctggattgct cctttcatat
    14881 aatcgaaatc gtcgaatcca ctaataaata tgagacgagt ccaaggaagt agtttccttg
    14941 cttcttttga aaaagtcaac ccgtccatga aaggcatttg gatatcagtc agaatgatgt
    15001 cgggctgtaa gtcgataatt gaagacagtg ccatctcacc atctccagct tcgccgacat
    15061 aagtgattgg ataataagaa ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga
    15121 ggtattcgtc ttcgatgata aaaactttat acatgtttgt ttcctccaat tgttttggga
    15181 gatagaggga tcatcagtgt gacagttgtt ccttctcggt atttgctgtc aatcgtgata
    15241 ggtttttgat catttccgta ataaagaagg agacgcttgt ttacgttgta aagtccgtag
    15301 ccgatactga aatctgaatc gatccctttg gctaactctt tacgtatttc tgccagtttt
    15361 tctgcaggga tgccaatccc gttgtctata actttgaatt ggatcatctc atcaattttg
    15421 catgtggtaa tacgaataaa tcctttgtga cggacgtact tcgttccatg gtatacagca
    15481 ttttcaatca atggctgcaa gatcattttt aaaatagaaa caggaaaaag tgtttctggt
    15541 atatcgatct tatagtcaag catatggccg tatcttattt ttaagatgac tagataatca
    15601 ttgatatggc taatttcttt ttcaagagga atccagtctt gtcctttgct taaagacgta
    15661 cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt cttcaaagca gtcttgttcg
    15721 atcaaagaaa taatggcatc caaactgtta taaataaaat ggggagtaat ctgagcttgc
    15781 aatacacgaa cttcactttg tgctaaatgg tattgtttca aagcgttctc ccgtaaagac
    15841 tggtttagat cttccgccat tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt
    15901 tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa gttgttctga catctcgatc
    15961 agctgattga tcggttcttg gacctttctc tctaaaaagc gacgattctt tttcgcatag
    16021 aaaaaaatgg tactaagaac tccaagttcc actattgtta atagaatgag cgagcggaca
    16081 agtgtcgtat tttttttgct tgctaagtcg atctccacac gaacaaaatc ttgtaagata
    16141 tctgacagca gttgagtgac agaatctacc tgattgatga tctcttcatt ggtatccata
    16201 aaatcttttg ccaagatatt attgatcagc tcgttttgat aatcttccag tgtatcgatc
    16261 gtacgtagcg atacatctaa cgtactaatt tctttaagtg tatttgtatt atttttgatc
    16321 tctcgaatat cattcctaag ttcttctagg atattttcct cattgaactc atctacggtt
    16381 acttggccag aaatcaagtc ccacatactt tccaaaactt tttgatcaac cttcaaagca
    16441 attgcatctg ctttttggat atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa
    16501 tataacagta tgctcagtag caaagggata acagcaaata gaagcatggc tttgtttgct
    16561 tgggcaaaaa tctgttttat gcttaaattt tttgtcatga tacttctcca gtcaataatt
    16621 tctggttggg atgttagcta tattctgctg ggaaaagaca gtctcggaaa taaaaatatc
    16681 tttaggtaat gtttttcctg tttgaatatt tccaaaataa cgagtaacgg tattggctac
    16741 ataccagccg acatcaggtt tacactcgac tacacaattg acgattcctg agcgtagctt
    16801 ctcgatcata tcttcttgtc catcaatagt tacaatgatc aaatcttctc caggtttgat
    16861 tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt tcatcactat ggctgtataa
    16921 tacatcgatt tcggataaat catttgtttc taaatatcgt ttcatctggt cttttccttt
    16981 catccgaata aaatcaccga caattcggtg aacaatagtc atcggaggtt tatggtcgat
    17041 ggttccatct ttttttaggg ttggtttacg attgattgat tccaaaaatc cttcggagcg
    17101 tagcttggtc ggtgtggagt tctctgaacc agcaagttct aggattctga tttcctgttt
    17161 ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa
    17221 actggggcca atgtgggtga cgaaaagctc gggatcattc gtttgaatat gtctgtcgac
    17281 aagtatgaca gggatccctg ctcgctttgc ttcttttaat acagagtccc acccggattc
    17341 ttgcaaagga gtaaaaatga tcatatccac tttataggca atgaacgtcc gtatatcttg
    17401 gatttgccgt tcctgattca tgtagccatt tcggtagaga acttggtaat cttctttttc
    17461 aagcgcagtg ataattgatt cagtatgctt tttccgccag ctactctccg ttcctgattg
    17521 cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta ttaggtgtac accccatcat
    17581 ttggtaagcg attacaacaa gggctaaaaa aaataaaaca cgtttcactt taaatcactc
    17641 catctggatt tgttagtact atcttatcag ataaaaggga aaatcaaagt caaaaggaat
    17701 caatcgaaaa aaatccaaga aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa
    17761 atttaaagta aatcccataa aaaaatcaat gttaacgctt tctttattag ctatactcgg
    17821 tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat ttagaaagat tgttgggact
    17881 atcgctattt tgaccgcaag cattctatta agtgcgtgtg gcaattcagg atctgcagat
    17941 gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat cagcagaaag atggatcgct
    18001 gatgggaaca acatggtctc ggaattagaa aaactgggtt ataaaacaga tcttcaatat
    18061 ggagaagata aagtagaaaa tcaagtagca caaatagaaa atatgattac aaaaggggta
    18121 gatacacttg tgattgcttc tatcgatggt tcggctttaa ctgatgtgtt agctaaagca
    18181 aaagaagcag atatcaaggt gatcgcctat gatcgtttac tgatgaattc agaaaatgtc
    18241 gattattacg cgacttttga taattttgga gtaggtgtat cgcaagcagc ttatattgaa
    18301 gagcacttag gattgaaaga aggaaaagga ccattcacga ttgaattatt tggtggctcg
    18361 cctgacgaca acaacgcact aatcaattac aacggagtga tgtctgtttt acaaccatat
    18421 atggacaacg gtcagttggt cgtgccatct ggacagacga gtttcagtca aatcgctacg
    18481 cttcgctggg atggttctac ggcgcaagca aggatggata atctgttaag cgcaaattat
    18541 actgaccaaa cattggatgc tgttttgtcg ccatatgatc caataagttt aggaatcatt
    18601 tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac ctttacctgt gattactgga
    18661 caagatgcga cagttgctgg cgttaaatcg atcattgccg gggaacaaac acaaacgatc
    18721 tttaaagaca cacgtatctt agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat
    18781 ggagaagagg ttccggtcaa tgatactgaa acttatgata atggggtgaa aactgtccct
    18841 acgtatttag caaatacagt atctgtcgat aaagataatt atcaagcaga gctgatcgac
    18901 accgattatt ataaagaatc cgatctgaaa aattaagagg aaccaaacaa ggaactaaag
    18961 agatctaagt cagagagagg ataagaatta tggcggattg tattttaaaa atggcagata
    19021 tcaccaaaaa attttctgga atccgagcgt tagattgcgt gaatctgatg attgaacgtg
    19081 gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa atcaacattg atgaatgtct
    19141 tgagcggctt gtatccatat ggctcctatg acggggaaat cttttatgat ggaaagctgt
    19201 gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat cgtcatcatt catcaagaat
    19261 tagcactaag tccttatctt tcaataaaag agaatatttt tctcggaaat gaacaagcag
    19321 aaagaggcgt gattgattgg gaactgacag aaaagaaaac atatgaactg ctgaataaag
    19381 tcggtttgtc tatcgacccg aatcttctag tatcccagat tggcgtaggg caacagcaac
    19441 tagttgaaat agctaaagca ttatcaaaat ccgtccgtct gttgattttg gatgagccta
    19501 ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag attgatccga gagttgaaag
    19561 agcaaggtgt aacctccatc attatttcac ataagctgaa tgaaatcgtg gatatcgcag
    19621 atcgaatcac cattttacgg gatggacaga cgattgagac actagaaaat gaaatgatca
    19681 gcgaagagca tattattcgc ggaatggtgg gcagagattt agttgatcgt tatcctgaaa
    19741 gaaaaccgga tattggagaa gtttatttta aagtagaaga ctggacggtc tatcaccctc
    19801 ttgatcacga gcgaatcata aacgaccaca tcaacttgca tttgaaaaaa ggagagattg
    19861 taggaatagc tggactgatg ggagcgggca gaactgaatt cgctatgagt ctatttggac
    19921 attcttatgg aagcaagata tccggacgta tattaaagga aggggcagag atatcagtta
    19981 agaccgttcc ttcagccatt gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt
    20041 taggactgaa tctgctgatg gatattcggg aaaacgtgac gctgagcagt ttggataaaa
    20101 taagtcaaaa aggagtatta gataaagaaa aagaagtaac tgaggcagaa aatttccgga
    20161 aaaagatgcg tataaaaaca aactccatcc atcaaattgt cagtagtctt agtggtggga
    20221 atcaacaaaa agtcgtttta tccaagtggc tgatgacaca acccgatatt ctttttttgg
    20281 atgagccaac ccgtgggata gatgttgggg caaaatatga gatctacacg attatcgaag
    20341 aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc tgaacttcct gaaatattag
    20401 ggatgtgtga tcgaatctat acgatgaacg aaggacgtat tacaggagaa gtctcgcgag
    20461 aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa agaagaggtg ggttaagttg
    20521 gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac taaatttgaa aagtagagca
    20581 gctgatcttt ttggtcgcta cagcatggtg atcattttag cgggactact tattgcgttt
    20641 caattgatga cggatggtat tttctggcgt ccattgaata taactaacct tgttctgcaa
    20701 aatagccata tattagtact agctgcaggg atgttattag ttgtgctctt aggtcatgtg
    20761 gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc
    20821 aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc
    20881 ggcgcatggc aaggtttttg ggtagcttat gcagggatac cggcgtttat cgtgacttta
    20941 gcaggattgc tgatgtttcg tggactgaca caagttgttc taggaggcca atctctagca
    21001 ccttttccag ctgttttcca aaaaatgtct acgggttatt taccagactt aagcgaagga
    21061 agcagccttc acttcttgac attgatttta ggaatgatca tcacgatcgt gttagttgtt
    21121 ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat ttgaagtgga atctccaaca
    21181 gctttcttgg taaaggcggt atttacagca gtcgttgtac taggaataac ttatatcttt
    21241 gcctcttatc aagggtatcc gattattttg atcattcttg gtttgatcgt tgcagcatat
    21301 gggtttttga caaataaaac agtagcaggt cgacagattt atgcgacggg cggaaatcga
    21361 aaagcggctg aactatcagg gatcaaaaca aagcgaatca cgttctgggt attcgtcaac
    21421 atgggagtca tggcagcttt agctggatta gttttagctg ctcgattgaa tgcggcaaca
    21481 cctcaagcag gtacatcgat ggaactggat gcaatggcgg cagtatattt tggtggtgct
    21541 tcgacttccg gcggcattgg aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt
    21601 gtattgaaca atggcatgtc gattttaggc gtcggggtag actggcagca ggcaatcaaa
    21661 ggattgatct tgcttttagc cgttgtgcta gacttgtata ataagaaaaa acgaacaacg
    21721 taggagaacg ccggttattc agaaaaaaga ctgtgacaag acatttgtca cagtcttttt
    21781 ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac taaaaataat atatgttttt
    21841 ttaccattcg tataaattct ctttttgctc ttgatttttt tgcttaattg agttaaatta
    21901 catagggcgc taaaaaaagc cttgattaaa ttaataatgt tcgtgttttg ctggatgtga
    21961 acatcaggag gataatgtaa tgaaagtatt tgattacgaa gatattcaat taattcctaa
    22021 taaatgtatc gtaaatagcc gttctgaatg tgatacaacg gtcacattag gtaaacatac
    22081 attcaaaatg ccagtagtgc ctgccaacat gcagacgatt attgatgaaa caatcgcaga
    22141 attcttagcg gaaaatggtt atttctatat catgcatcgg tttgatgaag cagctcgaat
    22201 tccgtttatc aaaaaaatga aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa
    22261 gaaagaggaa tattccttta tagagaagtt ggcagaagaa tcgctaaatc ctgattatat
    22321 cactatcgac atcgcgcatg gacatgctaa ctcagttatt gatatgattc agcatattaa
    22381 aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg
    22441 tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg attggtccag gaaaagtatg
    22501 tattacaaaa atcaagacag gctttgggac tggaggctgg caactggcag ctcttagatg
    22561 gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt gggatcagaa cacatggcga
    22621 tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg attggttcat tgtttgccgg
    22681 tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga atcgtgtata aagaatactt
    22741 tggttctgct tcagaattcc aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg
    22801 gatccaacac aaaggatctt tgaaagatac actagtggaa atgcaacaag accttcaatc
    22861 atctatttct tatgctggag gaagagatct ggaagccatt cgaaaagttg actatgtcat
    22921 cgtgaaaaat tcgattttta atggagacac aatctaaaaa ataaataaaa tattaggatg
    22981 ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc aaacaaaaat agttctttat
    23041 atgttttgtt tggctggaat tattatcgca gatacaagct tttgaacatc attattcaga
    23101 ataagggact gtgataagat ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc
    23161 ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt
    23221 atgtgggaaa aagtagagaa aaaacaagga ccgatttatc gtcaaattat ggaacagatt
    23281 atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc aacttgaatc agaacgaaaa
    23341 ctagggcaac tttttggtgt gaatcgttcc accgttgtcc atgcattgga agagcttagg
    23401 gagcttgggg tactaacgag caaacgtggc agcggatggt ttgttaatca gactgaatgg
    23461 gggaaattcg ctgttccaag aatcgattgg cggcaaatga tctctcctcg ttatgagcag
    23521 acggactggt atgagcaaaa aataagagaa gcaaagcaga tccaaaaaac atcgtttctt
    23581 gatttatacg ctagcgagat gccagatgag cttttgccga attttgagtt tccttcttat
    23641 tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc ttttaggcta tactccgtta
    23701 cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat tggcattttc ccccaaccag
    23761 cttcttgtga ctggtggcgg tcagcaagca atctttttga ttttacaaac gattctttca
    23821 gctggagagg ggatagctgt agagtcacct tctttttttt accgattacc tcttttcaaa
    23881 gcttcaggaa cgcgtttatt tgggataccg atggatgaag aagggatcga cttagaaaaa
    23941 ctgaaagaat cgatccataa aaataaattg aaagcagtgc ttatcaaccc gaattttcaa
    24001 aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga
    24061 cagtatcggc ttccaattat tgaagacgat gtatttacgg atctttcatt tctaaaccaa
    24121 gaagaagtcc ttcctatacg aacgattgat ccggataatg tgttatacgt cggttcgtta
    24181 tctagggttt taggaaaaac aacgaaaatc ggttgggtga taggacctga gacttttatc
    24241 tcgcaactag ctcaagcgca agaaatgatg gagttttcga tgaatatcct tacacaaatc
    24301 acagtggcta atgtatttaa tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag
    24361 ttgaaagaaa aaagccgact gataagcgct tggggagatt cacaatcgtt tttcaagctt
    24421 cattcaattt taggtggtta ctatgcctgg attacttggg atgggaaaaa actagacccc
    24481 gaactagcag aaaaagtagt agatatgggt cttggaattg caccaagtct actttttggg
    24541 caagatataa atggaatcag aatcaattac agtaggctgg atgaaaacca aataccgttg
    24601 ttttattcaa agatgaaaat tctagaaaag tggttaacag aataatttca caaagtaaat
    24661 agattaaaga atataacagc ttggatttaa atttcaagct gtttcttttt tttcggaaaa
    24721 agaatacgat ataaagaaac taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt
    24781 aaataaaata aatgtttgca acaaaactgt ataacaccaa cgtttttaaa tagaa
//
LOCUS       DAZKNM010000019        24611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000019 DAZKNM010000000
VERSION     DAZKNM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.019"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8M9_000664"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8M9_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0377458.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8M9_000665"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8M9_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0377459.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8M9_000666"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8M9_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0377460.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8M9_000667"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8M9_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377461.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8M9_000668"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8M9_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0377462.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8M9_000669"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8M9_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0377463.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8M9_000670"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8M9_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377464.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8M9_000671"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8M9_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0377465.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8M9_000672"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8M9_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0377466.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8M9_000673"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8M9_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0377467.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8M9_000674"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8M9_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0377468.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8M9_000675"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8M9_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0377469.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8M9_000676"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8M9_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0377470.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8M9_000677"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8M9_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0377471.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8M9_000678"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8M9_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0377472.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8M9_000679"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8M9_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0377473.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8M9_000680"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8M9_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377474.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8M9_000681"
     CDS             13967..14695
                     /locus_tag="ACG8M9_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0377475.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8M9_000682"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8M9_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0377476.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8M9_000683"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8M9_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377477.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8M9_000684"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8M9_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377478.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8M9_000685"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8M9_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0377479.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8M9_000686"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8M9_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377480.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG8M9_000687"
     CDS             16394..16606
                     /locus_tag="ACG8M9_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377481.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG8M9_000688"
     CDS             complement(16639..16833)
                     /locus_tag="ACG8M9_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377482.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG8M9_000689"
     CDS             complement(16846..17028)
                     /locus_tag="ACG8M9_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377483.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG8M9_000690"
     CDS             complement(17025..17165)
                     /locus_tag="ACG8M9_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377484.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG8M9_000691"
     CDS             complement(17655..18020)
                     /locus_tag="ACG8M9_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377485.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG8M9_000692"
     CDS             complement(18060..18362)
                     /locus_tag="ACG8M9_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0377486.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG8M9_000693"
     CDS             complement(18362..18661)
                     /locus_tag="ACG8M9_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377487.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG8M9_000694"
     CDS             complement(18654..18818)
                     /locus_tag="ACG8M9_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377488.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG8M9_000695"
     CDS             complement(18824..19093)
                     /locus_tag="ACG8M9_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377489.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG8M9_000696"
     CDS             complement(19105..19854)
                     /locus_tag="ACG8M9_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377490.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACG8M9_000697"
     CDS             complement(19851..20543)
                     /locus_tag="ACG8M9_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0377491.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACG8M9_000698"
     CDS             complement(20549..21220)
                     /locus_tag="ACG8M9_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0377492.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACG8M9_000699"
     CDS             complement(21213..21554)
                     /locus_tag="ACG8M9_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377493.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="ACG8M9_000700"
     CDS             complement(21732..22430)
                     /locus_tag="ACG8M9_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0377494.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..22987
                     /locus_tag="ACG8M9_000701"
     CDS             22517..22987
                     /locus_tag="ACG8M9_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377495.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23030..23209)
                     /locus_tag="ACG8M9_000702"
     CDS             complement(23030..23209)
                     /locus_tag="ACG8M9_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377496.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23196..23534)
                     /locus_tag="ACG8M9_000703"
     CDS             complement(23196..23534)
                     /locus_tag="ACG8M9_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0377497.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23550..23753)
                     /locus_tag="ACG8M9_000704"
     CDS             complement(23550..23753)
                     /locus_tag="ACG8M9_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377498.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23768..24544)
                     /locus_tag="ACG8M9_000705"
     CDS             complement(23768..24544)
                     /locus_tag="ACG8M9_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0377499.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
BASE COUNT         6825 a         5160 c         3802 g         8824 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt
    22981 aaactgagta atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc
    23041 cctcggttgg ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc
    23101 aaaccatgta aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt
    23161 agatgattag cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc
    23221 cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga
    23281 tatgtgtctc ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg
    23341 ataatatttc tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg
    23401 ttaatccgtt ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt
    23461 aactcctcat aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct
    23521 tctagatatt gcattagctg ttcctccctt tacaattcgt acatagtgat aatcgaatct
    23581 ataattctgt ttgcttctgc agaagtcttt ttaccgttta aaattaaaga taagtagctt
    23641 ttgctaattc caaatctttc agcaagcatg gtgtaagtta agaactttga actttcgaca
    23701 tattctttga ttttttctct atctcgttga gtgatttctg caatgtcagt catactaaaa
    23761 ctcctttcta accaatttcc tctaaatcca tttgagggta atatccttct ttttttagta
    23821 attcgtagat aaatagacgc cctttctgtg tccatttggt attcattaca attttagtgc
    23881 caccatcggc tttcgggatc tcagttgtat gagattttgt gtatccttgg ttcatgtgtt
    23941 ttttgcacaa taaccattgg ttaccgactt ttttctgaat acctagttta tgaagtaatt
    24001 tattcatctg ttgtggagac atcccataat ctgctgcaat ctgactaatt gttactgaat
    24061 ctgtagaaga taatatgcta tctaaatagg agattttagg ttcgtactct gcaatctttt
    24121 gttctgcgat taatcttcca gttctttctt cttttagttg agttgctaat tgaatgattg
    24181 tatctggatt aagcaaagct tcttctactt tttctggagt tagataacct ccatgttttc
    24241 taattgctgg caacacttca cttgttaccc aacgtttaaa ttttttggca gagggaagtt
    24301 ttgattttaa gattaaactg tacaaaccag attcgttgat gattgtcatc tctcttgatt
    24361 gacctgaggt cgtgattcgc gaccccatct tatcttccaa atctacatgc cgcgacaaag
    24421 catctttagt gtttgaataa cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt
    24481 catcatttac taaaattgtc cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt
    24541 tcatatttct cattcctttc ttttatattc gtaaacaaat ttaacaactt ttttctaaat
    24601 tcaattgaca g
//
LOCUS       DAZKNM010000020        24327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000020 DAZKNM010000000
VERSION     DAZKNM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.020"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..2467)
                     /locus_tag="ACG8M9_000706"
     CDS             complement(122..2467)
                     /locus_tag="ACG8M9_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0377500.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2746..3648
                     /locus_tag="ACG8M9_000707"
     CDS             2746..3648
                     /locus_tag="ACG8M9_000707"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0377501.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3859..5634
                     /locus_tag="ACG8M9_000708"
     CDS             3859..5634
                     /locus_tag="ACG8M9_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0377502.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5700..7286)
                     /locus_tag="ACG8M9_000709"
     CDS             complement(5700..7286)
                     /locus_tag="ACG8M9_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0377503.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7537..7761
                     /locus_tag="ACG8M9_000710"
     CDS             7537..7761
                     /locus_tag="ACG8M9_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0377504.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7903..9654
                     /locus_tag="ACG8M9_000711"
     CDS             7903..9654
                     /locus_tag="ACG8M9_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0377505.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9654..11438
                     /locus_tag="ACG8M9_000712"
     CDS             9654..11438
                     /locus_tag="ACG8M9_000712"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377506.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11773..12625
                     /gene="licT"
                     /locus_tag="ACG8M9_000713"
                     /pseudo
     CDS             11773..12625
                     /gene="licT"
                     /locus_tag="ACG8M9_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12722..14608
                     /locus_tag="ACG8M9_000714"
     CDS             12722..14608
                     /locus_tag="ACG8M9_000714"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0377507.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14649..15551)
                     /locus_tag="ACG8M9_000715"
     CDS             complement(14649..15551)
                     /locus_tag="ACG8M9_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0377508.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15781..17184
                     /locus_tag="ACG8M9_000716"
     CDS             15781..17184
                     /locus_tag="ACG8M9_000716"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0377509.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17184..18617
                     /locus_tag="ACG8M9_000717"
     CDS             17184..18617
                     /locus_tag="ACG8M9_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0377510.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18762..19688
                     /gene="trxB"
                     /locus_tag="ACG8M9_000718"
     CDS             18762..19688
                     /gene="trxB"
                     /locus_tag="ACG8M9_000718"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0377511.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19901..21628
                     /locus_tag="ACG8M9_000719"
     CDS             19901..21628
                     /locus_tag="ACG8M9_000719"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0377512.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21644..21994
                     /locus_tag="ACG8M9_000720"
     CDS             21644..21994
                     /locus_tag="ACG8M9_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0377513.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22088..22948)
                     /locus_tag="ACG8M9_000721"
     CDS             complement(22088..22948)
                     /locus_tag="ACG8M9_000721"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0377514.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23040..23468
                     /locus_tag="ACG8M9_000722"
     CDS             23040..23468
                     /locus_tag="ACG8M9_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0377515.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23630..23806
                     /gene="rpsU"
                     /locus_tag="ACG8M9_000723"
     CDS             23630..23806
                     /gene="rpsU"
                     /locus_tag="ACG8M9_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0377516.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23833..24279
                     /locus_tag="ACG8M9_000724"
     CDS             23833..24279
                     /locus_tag="ACG8M9_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0377517.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7706 a         4351 c         5185 g         7085 t
ORIGIN      
        1 tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca tttattcgga
       61 aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt aatctattag
      121 atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca taagctgaag
      181 cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat ttaaaggcgc
      241 ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc gtttggatgg
      301 attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat acttttttct
      361 tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca gggtttgctt
      421 ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt gcttggctgt
      481 ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat ggagttgaga
      541 cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc caatctgcat
      601 ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg attttttcat
      661 tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa taaatacgga
      721 cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg gaatcaatga
      781 tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt gtctgttgaa
      841 gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta tcatttgctg
      901 ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca ccggaattca
      961 acacatcttg atataagtga taagctggga tattgttgga ccattccagt gactcacgga
     1021 cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca ccagcatttt
     1081 ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg acaagtccca
     1141 tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct tgacgttctg
     1201 tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg aagccataga
     1261 tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta ccagcaccat
     1321 catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag atatctttat
     1381 cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct ttttgataat
     1441 aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc aattgttgtg
     1501 caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg tcagtctgtt
     1561 ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct tcttcgtatt
     1621 gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct tttctggcaa
     1681 gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta tacgtgatag
     1741 ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc tttgcatcta
     1801 caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc ttattattcc
     1861 gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa aagtgttttt
     1921 ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt tcatcggtta
     1981 ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag ccgatgccag
     2041 tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga tgtttatcga
     2101 agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc ttatcagatg
     2161 aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta tctgcataaa
     2221 caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt ttagtcggca
     2281 attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata cctagagctg
     2341 cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact ctaaaaataa
     2401 tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga gtatttttgc
     2461 gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca aataatagga
     2521 caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag ataaaacgtg
     2581 aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa gtttcagtta
     2641 ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc ttatcaatat
     2701 acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt attaggctca
     2761 catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc agccagttac
     2821 ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag aaaagctatc
     2881 gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact aacaaatatc
     2941 gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga aatgtttcca
     3001 tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg tgctactcaa
     3061 atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat tgctagaatt
     3121 attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc tttggagacg
     3181 atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca aatcatcgat
     3241 ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac gaatgatgcc
     3301 ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa ggtcatcgga
     3361 ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg ttctcataaa
     3421 gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa caaaatcgtc
     3481 catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt cggtaaagat
     3541 aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc gcaagcattc
     3601 gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt tttctacctg
     3661 acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag aaacaaagga
     3721 gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa attcagacaa
     3781 acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt agcaaaacgt
     3841 gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca actaaaaaag
     3901 aaacatacat actttattct tggcatagcc gtattattta caattattcg gttgtattta
     3961 tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca gctgctgatt
     4021 cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac tacaacactt
     4081 tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca tctttcttat
     4141 gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact cgcgtttaaa
     4201 ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt gttttctccg
     4261 attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt gattccggct
     4321 gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg ttctagcaga
     4381 aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg gttcattcgc
     4441 gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag tttcgtccag
     4501 atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt
     4561 cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat agttggcttt
     4621 tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat tgcaaccatc
     4681 aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg aatagatgat
     4741 gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc actaaataaa
     4801 gcattagccg catctcctac attccatact gtttctaccc gtattgacga attatatacg
     4861 actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat catcttttgg
     4921 gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa agaaaccaat
     4981 gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg aaccatcaaa
     5041 aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag tgatctgcca
     5101 atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa agatttccat
     5161 caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca agaattatta
     5221 aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc taatttccaa
     5281 ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt tctggctatt
     5341 cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac cgtatttgca
     5401 aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt aatactgaca
     5461 caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga aaaaaaggac
     5521 tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat cgtagtaaca
     5581 agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa ataaaaagac
     5641 ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa aatcagtttt
     5701 tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt tcctttatat
     5761 aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac ataagcgatg
     5821 tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt
     5881 cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca gccaagaagt
     5941 tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt ggtcatcatc
     6001 agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga ttcattcatt
     6061 aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa caaaacacaa
     6121 atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca aatcgggata
     6181 cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt aatagtagag
     6241 ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc agattttgct
     6301 acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc atacagacca
     6361 aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat gatcaatagc
     6421 agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt agaaaaacgt
     6481 acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg tgaaactgct
     6541 gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa agctccagtc
     6601 agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt agggataaag
     6661 ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac aaaaacgaat
     6721 aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc gacttttgtc
     6781 agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac caatggaata
     6841 tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc tgttgccata
     6901 atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt ccgtgctcgg
     6961 attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat atactgtaaa
     7021 agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg gaaattttgt
     7081 ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc tactaatgca
     7141 cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg tacatccact
     7201 gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg atgcttatga
     7261 aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat ctacgattac
     7321 attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta attttttttc
     7381 gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa accactattt
     7441 tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta aaataaatca
     7501 ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg tattgtcgta
     7561 cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa
     7621 tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg
     7681 atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat gcagcaaatg
     7741 aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg attttttcag
     7801 tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa aagacaagca
     7861 cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat ttttagaaaa
     7921 ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt ctccctattc
     7981 gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat tgatgagatc
     8041 gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt gatccttgca
     8101 gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg aagtgcagct
     8161 cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat ggacaatctc
     8221 ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga tttgaatgca
     8281 atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat tacgggcgga
     8341 acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat tgcgctgatt
     8401 cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca cgatgctttt
     8461 cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag tatcactggg
     8521 atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt tgaagaaaag
     8581 attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt atttgatcca
     8641 ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg tggaaagtat
     8701 gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta tatcggtatg
     8761 cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag aggaaatgct
     8821 tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga acaactgggt
     8881 gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt taactatccg
     8941 aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg agaaacatta
     9001 ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact gatgcgtgaa
     9061 tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga atattcattg
     9121 aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt ttcgatgacc
     9181 attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt agagcatgcg
     9241 gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta tgatacctta
     9301 gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc gattgctcga
     9361 gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc agtagatgcc
     9421 aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa aacaaccatc
     9481 attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa
     9541 gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg ctggtatgaa
     9601 cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc ctgatggatg
     9661 aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag aagatcatca
     9721 aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct atcttatttg
     9781 cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact tttatggatg
     9841 atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct ggtgtgtact
     9901 tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg tattccatgg
     9961 cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg catacactag
    10021 gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg acgaatgaca
    10081 cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg atctttgcag
    10141 tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg tggactttgg
    10201 tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt tctcgtattt
    10261 accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa tcgatttccg
    10321 gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa tttgaacaag
    10381 tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg attctgttaa
    10441 gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact ttgtttggga
    10501 tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg acctatgtcc
    10561 aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt acagatggga
    10621 tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca ccgcaacaaa
    10681 atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat gtaagctttt
    10741 cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat ccgggtgaga
    10801 ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac gtattgatgc
    10861 ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt cgagaatatc
    10921 caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc ttgttttatg
    10981 gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa cagattcgcc
    11041 atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa ggatatcact
    11101 caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg atcacttttg
    11161 cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca gcaaatatcg
    11221 atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa ggacggacaa
    11281 cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt ttagttttag
    11341 acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa aatggtttgt
    11401 atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg acagtagcat
    11461 agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca gattcttcat
    11521 ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc tagtcaaaag
    11581 cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa tataaagaaa
    11641 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    11701 tccatggacg gctttttggg tttttctttt tgttttggat acagccctat atatcatgga
    11761 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    11821 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    11881 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    11941 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    12001 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    12061 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    12121 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaac
    12181 agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag ccggatttat
    12241 cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata cctacctgtt
    12301 cactataaca atacaggata ttctaaatat cgtccgctat tactataaga ttacatttga
    12361 tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc tgacacgcgt
    12421 cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc aggttattca
    12481 aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt tagccgagac
    12541 gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata ctgcgcgttt
    12601 agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca agaacgtatg
    12661 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    12721 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    12781 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    12841 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    12901 gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    12961 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    13021 tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg ctgacgggat
    13081 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    13141 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    13201 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    13261 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    13321 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    13381 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    13441 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    13501 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    13561 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    13621 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    13681 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    13741 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    13801 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    13861 tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta tttttacctg
    13921 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    13981 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    14041 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg cgctgacttt
    14101 tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat caggtaaaga
    14161 aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa aggatgcagc
    14221 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    14281 tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa tcgggatggt
    14341 ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc agttggaagg
    14401 gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg atctgttgct
    14461 tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac ctgtgattgt
    14521 catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg tcgggccaga
    14581 cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac taggcttaac
    14641 tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat gatggctgag
    14701 ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa attctgttga
    14761 taattttctg caaaaacaca actatataac acgtctagaa gataacaaat cgatgtattg
    14821 atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg caaagcggct
    14881 gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat tggcacatga
    14941 ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc
    15001 agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc accaaaagtg
    15061 gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc
    15121 aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg
    15181 gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt catctgttct
    15241 aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc
    15301 gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga
    15361 aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt ggacgggtgg
    15421 acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg agaatcgctt
    15481 aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca
    15541 cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac ttttttgaaa
    15601 atgatttgta aacggattta tgttatatag cttaataaag acattttttg acaagatatt
    15661 tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga aacgaatttc
    15721 tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag gagtgagaac
    15781 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    15841 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    15901 gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg tatgtgggat
    15961 gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc attgaaagat
    16021 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    16081 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    16141 aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa gcgtttatgt
    16201 ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga agcagttatc
    16261 agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat ggcgatgatg
    16321 acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    16381 aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    16441 attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga catcccgttt
    16501 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    16561 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    16621 cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc agaattggat
    16681 cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc tgcctgtgaa
    16741 acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    16801 ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    16861 caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg ctggttgcaa
    16921 gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga ttatggaaca
    16981 gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc aaaacggatc
    17041 atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga aacaatcagc
    17101 cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt atcaagcaat
    17161 acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg ggcggagcta
    17221 cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta gcgaacgtag
    17281 atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag aaaatgtttg
    17341 aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat catcattata
    17401 aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg tccattgctt
    17461 ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg ttgaaatttt
    17521 atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg acgattacac
    17581 attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    17641 ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga cttgtcaaat
    17701 attggttgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    17761 tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    17821 tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag aacaaagtag
    17881 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    17941 cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    18001 atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa atggaacctg
    18061 gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct tattattctt
    18121 ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    18181 ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat ccattaggct
    18241 tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt attgtagaaa
    18301 acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat gattatcgaa
    18361 tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta gatggagtcg
    18421 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt acaggtgaga
    18481 tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat ggcacattag
    18541 cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    18601 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    18661 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    18721 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    18781 ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    18841 tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca gaaatcgaaa
    18901 actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg tatgaaggtg
    18961 tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag gatcacggca
    19021 gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc attatcgcaa
    19081 caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgtggcg
    19141 tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta gtagtgatcg
    19201 gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    19261 tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    19321 cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaatgaaa
    19381 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt gatttggcag
    19441 cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt agagaagctg
    19501 ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg aatataccag
    19561 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact actgctgttg
    19621 gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta gaagaaacaa
    19681 ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact cttttttttg
    19741 cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga taaaagaatc
    19801 ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag tgaatgctat
    19861 acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg aacaagtgta
    19921 tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat taaatgatct
    19981 gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat tcggtactgc
    20041 aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata cggtaagaca
    20101 agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa aacgccgagg
    20161 tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg aagcagccaa
    20221 aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc caacaccaga
    20281 gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa ctgcatccca
    20341 caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac aaatgcctcc
    20401 tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt taaaagttga
    20461 agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg gagaagaagt
    20521 ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat tggtggaaac
    20581 aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa aaatgttggg
    20641 cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag aacaagctgt
    20701 cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact ctgctttcga
    20761 gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa cggatcctga
    20821 tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag tattgacagg
    20881 aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat cgcgaggcac
    20941 tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc ctacagcaat
    21001 tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat tcattgctga
    21061 aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat tcgaagaaag
    21121 ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag cattgctctt
    21181 attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg acggcttgca
    21241 aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga caatgagcgg
    21301 acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag aagcaccgac
    21361 agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga cgagaacaga
    21421 ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga agtatacgct
    21481 ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa tcaaatttta
    21541 tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg cttatgaagc
    21601 gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa aagtggtaaa
    21661 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaaac
    21721 aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc gtctcaaaat
    21781 tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtagaaat
    21841 ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg tcgtcaagtc
    21901 ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac tggatatttt
    21961 gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag agacaaactg
    22021 gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa aaagacaaga
    22081 aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga tcttctaggt
    22141 ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg acattgatta
    22201 ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct cgtatttttt
    22261 cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt tctttacgga
    22321 tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga tcgatctccc
    22381 aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc tttaaatttt
    22441 tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct agcaaaacga
    22501 catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca gcaaattcca
    22561 ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca ggttgccgaa
    22621 tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc acatcaaaac
    22681 taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct tgattgatca
    22741 atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc atcaatgttt
    22801 tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga tattgggcca
    22861 tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg acaaaaaaag
    22921 taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt tttttatgtt
    22981 tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag gaataagtca
    23041 tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac ggttttaaat
    23101 atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc tacgtatccg
    23161 ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac gatacgattt
    23221 atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat ttaaatggag
    23281 agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat cactttatct
    23341 gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat ttcgaagaac
    23401 agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga tgtgaaaatt
    23461 gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca agatttcctt
    23521 gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt tgagatatat
    23581 ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca tgtcaaaaac
    23641 agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac gttccgtgtc
    23701 aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac caagtgtaag
    23761 acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct gtgatgaatt
    23821 ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac agcgatgaaa
    23881 gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat acaaaacgag
    23941 cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt gtctcgcgaa
    24001 atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga cgatttagct
    24061 gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca actttccgac
    24121 gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc atcaatgaaa
    24181 gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc agatggaaac
    24241 caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt aaaggctctt
    24301 cgtcaatgag tgttggtgaa gaaattc
//
LOCUS       DAZKNM010000021        24275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000021 DAZKNM010000000
VERSION     DAZKNM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.021"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..790)
                     /locus_tag="ACG8M9_000725"
     CDS             complement(56..790)
                     /locus_tag="ACG8M9_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0377518.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="ACG8M9_000726"
     CDS             1117..1527
                     /locus_tag="ACG8M9_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0377519.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="ACG8M9_000727"
     CDS             1520..2221
                     /locus_tag="ACG8M9_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0377520.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="ACG8M9_000728"
     CDS             2221..3807
                     /locus_tag="ACG8M9_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377521.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="ACG8M9_000729"
     CDS             3826..4047
                     /locus_tag="ACG8M9_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0377522.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="ACG8M9_000730"
     CDS             4044..4805
                     /locus_tag="ACG8M9_000730"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0377523.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="ACG8M9_000731"
     CDS             5185..5694
                     /locus_tag="ACG8M9_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0377524.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="ACG8M9_000732"
     CDS             complement(5769..6308)
                     /locus_tag="ACG8M9_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0377525.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ACG8M9_000733"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ACG8M9_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0377526.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="ACG8M9_000734"
     CDS             7321..8172
                     /locus_tag="ACG8M9_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0377527.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="ACG8M9_000735"
     CDS             8200..8835
                     /locus_tag="ACG8M9_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0377528.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="ACG8M9_000736"
     CDS             8855..9628
                     /locus_tag="ACG8M9_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0377529.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="ACG8M9_000737"
     CDS             10036..10833
                     /locus_tag="ACG8M9_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0377530.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="ACG8M9_000738"
     CDS             10811..11224
                     /locus_tag="ACG8M9_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0377531.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="ACG8M9_000739"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="ACG8M9_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0377532.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="ACG8M9_000740"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="ACG8M9_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0377533.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /locus_tag="ACG8M9_000741"
     CDS             16001..16561
                     /locus_tag="ACG8M9_000741"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0377534.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ACG8M9_000742"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ACG8M9_000742"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0377535.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="ACG8M9_000743"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="ACG8M9_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0377536.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="ACG8M9_000744"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="ACG8M9_000744"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0377537.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="ACG8M9_000745"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="ACG8M9_000745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0377538.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="ACG8M9_000746"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="ACG8M9_000746"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0377539.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="ACG8M9_000747"
     CDS             21908..22486
                     /locus_tag="ACG8M9_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0377540.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="ACG8M9_000748"
     CDS             22538..23020
                     /locus_tag="ACG8M9_000748"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0377541.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="ACG8M9_000749"
     CDS             23137..23511
                     /locus_tag="ACG8M9_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377542.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23886..>24275)
                     /locus_tag="ACG8M9_000750"
     CDS             complement(23886..>24275)
                     /locus_tag="ACG8M9_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0377543.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7908 a         4353 c         5197 g         6817 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttatttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttgggagcg tcaataattt tgtgtaaata aattgtcctc ctgcaaaata
    23881 attagttact cagtaaacat tgaaactaat gtatcggtta cctgttgaaa acctttatgg
    23941 cttctgttta gaaatttttg attgtatgta tcaaaaatgc tgactagaaa gcgttctagt
    24001 gattcttcat tttgaaactg ctcttttcta cggctgtatc ttttaatttg cttattgaaa
    24061 gactcgatta gattggttga gtaaatggtt ctacgaatgc taggtggaaa atcataaaaa
    24121 gttaataagt cttggttttc tatgagtgac tgcgtcactt taggatagtt tttcttccat
    24181 ttctcaatca tgccggataa gaaggtattc gcttcttctt ttgagttagc ttgataaaca
    24241 gccttaaagt catcacagat ttcttttcgg tcttt
//
LOCUS       DAZKNM010000022        24258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000022 DAZKNM010000000
VERSION     DAZKNM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.022"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8M9_000751"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8M9_000751"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0377544.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8M9_000752"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8M9_000752"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0377545.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8M9_000753"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8M9_000753"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0377546.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACG8M9_000754"
     CDS             complement(3374..4681)
                     /locus_tag="ACG8M9_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0377547.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACG8M9_000755"
     CDS             complement(4684..5265)
                     /locus_tag="ACG8M9_000755"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0377548.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACG8M9_000756"
     CDS             complement(5837..7129)
                     /locus_tag="ACG8M9_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0377549.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACG8M9_000757"
     CDS             complement(7126..7749)
                     /locus_tag="ACG8M9_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377550.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACG8M9_000758"
     CDS             complement(7752..8588)
                     /locus_tag="ACG8M9_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377551.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACG8M9_000759"
     CDS             complement(8605..9492)
                     /locus_tag="ACG8M9_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377552.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACG8M9_000760"
     CDS             complement(9508..10791)
                     /locus_tag="ACG8M9_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0377553.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACG8M9_000761"
     CDS             complement(11474..12157)
                     /locus_tag="ACG8M9_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0377554.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACG8M9_000762"
     CDS             complement(12175..12792)
                     /locus_tag="ACG8M9_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0377555.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACG8M9_000763"
     CDS             complement(12802..13626)
                     /locus_tag="ACG8M9_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0377556.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACG8M9_000764"
     CDS             complement(13619..14179)
                     /locus_tag="ACG8M9_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377557.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACG8M9_000765"
     CDS             14353..15309
                     /locus_tag="ACG8M9_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0377558.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACG8M9_000766"
     CDS             complement(15614..16570)
                     /locus_tag="ACG8M9_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0377559.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACG8M9_000767"
     CDS             16674..17276
                     /locus_tag="ACG8M9_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0377560.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACG8M9_000768"
     CDS             complement(17364..18863)
                     /locus_tag="ACG8M9_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0377561.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACG8M9_000769"
     CDS             complement(18879..19403)
                     /locus_tag="ACG8M9_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0377562.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACG8M9_000770"
     CDS             19701..20450
                     /locus_tag="ACG8M9_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0377563.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACG8M9_000771"
     CDS             20441..21226
                     /locus_tag="ACG8M9_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0377564.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACG8M9_000772"
     CDS             21195..21911
                     /locus_tag="ACG8M9_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0377565.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACG8M9_000773"
     CDS             complement(22013..23032)
                     /locus_tag="ACG8M9_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0377566.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24052)
                     /locus_tag="ACG8M9_000774"
     CDS             complement(23051..24052)
                     /locus_tag="ACG8M9_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0377567.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5222 c         4224 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcgactga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAZKNM010000023        23587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000023 DAZKNM010000000
VERSION     DAZKNM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.023"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8M9_000775"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8M9_000775"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0377568.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACG8M9_000776"
     CDS             complement(1174..1869)
                     /locus_tag="ACG8M9_000776"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0377569.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACG8M9_000777"
     CDS             complement(1882..2598)
                     /locus_tag="ACG8M9_000777"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0377570.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACG8M9_000778"
     CDS             2928..3536
                     /locus_tag="ACG8M9_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0377571.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACG8M9_000779"
     CDS             3861..4184
                     /locus_tag="ACG8M9_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0377572.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACG8M9_000780"
     CDS             4366..5736
                     /locus_tag="ACG8M9_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0377573.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACG8M9_000781"
     CDS             complement(5866..7167)
                     /locus_tag="ACG8M9_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0377574.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACG8M9_000782"
     CDS             complement(7521..9512)
                     /locus_tag="ACG8M9_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0377575.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACG8M9_000783"
     CDS             complement(9656..9922)
                     /locus_tag="ACG8M9_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0377576.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG8M9_000784"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG8M9_000784"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0377577.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG8M9_000785"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG8M9_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0377578.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACG8M9_000786"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACG8M9_000786"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0377579.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACG8M9_000787"
     CDS             complement(14082..14603)
                     /locus_tag="ACG8M9_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0377580.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACG8M9_000788"
     CDS             complement(14606..15448)
                     /locus_tag="ACG8M9_000788"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0377581.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACG8M9_000789"
     CDS             complement(15441..16166)
                     /locus_tag="ACG8M9_000789"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0377582.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACG8M9_000790"
     CDS             16342..16638
                     /locus_tag="ACG8M9_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377583.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACG8M9_000791"
     CDS             17206..18798
                     /locus_tag="ACG8M9_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0377584.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACG8M9_000792"
     CDS             18811..19551
                     /locus_tag="ACG8M9_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377585.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="ACG8M9_000793"
     CDS             19719..21068
                     /locus_tag="ACG8M9_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0377586.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="ACG8M9_000794"
     CDS             complement(21196..21786)
                     /locus_tag="ACG8M9_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0377587.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG8M9_000795"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG8M9_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0377588.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6744 a         4757 c         4498 g         7588 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacattt
//
LOCUS       DAZKNM010000024        23546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000024 DAZKNM010000000
VERSION     DAZKNM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.024"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..837
                     /gene="purR"
                     /locus_tag="ACG8M9_000796"
     CDS             19..837
                     /gene="purR"
                     /locus_tag="ACG8M9_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0377589.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1340..2713
                     /gene="glmU"
                     /locus_tag="ACG8M9_000797"
     CDS             1340..2713
                     /gene="glmU"
                     /locus_tag="ACG8M9_000797"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0377590.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2807..3778
                     /locus_tag="ACG8M9_000798"
     CDS             2807..3778
                     /locus_tag="ACG8M9_000798"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0377591.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3784..3886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3952..4521
                     /gene="thiT"
                     /locus_tag="ACG8M9_000799"
     CDS             3952..4521
                     /gene="thiT"
                     /locus_tag="ACG8M9_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0377592.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4602..5375)
                     /locus_tag="ACG8M9_000800"
     CDS             complement(4602..5375)
                     /locus_tag="ACG8M9_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377593.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5642..7342
                     /locus_tag="ACG8M9_000801"
     CDS             5642..7342
                     /locus_tag="ACG8M9_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0377594.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7312..7530
                     /locus_tag="ACG8M9_000802"
     CDS             7312..7530
                     /locus_tag="ACG8M9_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0377595.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7550..8440
                     /locus_tag="ACG8M9_000803"
     CDS             7550..8440
                     /locus_tag="ACG8M9_000803"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0377596.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8458..8742
                     /locus_tag="ACG8M9_000804"
     CDS             8458..8742
                     /locus_tag="ACG8M9_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377597.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9229..9843
                     /gene="gmk"
                     /locus_tag="ACG8M9_000805"
     CDS             9229..9843
                     /gene="gmk"
                     /locus_tag="ACG8M9_000805"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0377598.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9848..10153
                     /gene="rpoZ"
                     /locus_tag="ACG8M9_000806"
     CDS             9848..10153
                     /gene="rpoZ"
                     /locus_tag="ACG8M9_000806"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0377599.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10406..12814
                     /gene="priA"
                     /locus_tag="ACG8M9_000807"
     CDS             10406..12814
                     /gene="priA"
                     /locus_tag="ACG8M9_000807"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0377600.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12829..13320
                     /gene="def"
                     /locus_tag="ACG8M9_000808"
     CDS             12829..13320
                     /gene="def"
                     /locus_tag="ACG8M9_000808"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0377601.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13313..14251
                     /gene="fmt"
                     /locus_tag="ACG8M9_000809"
     CDS             13313..14251
                     /gene="fmt"
                     /locus_tag="ACG8M9_000809"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0377602.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14251..15609
                     /gene="rsmB"
                     /locus_tag="ACG8M9_000810"
     CDS             14251..15609
                     /gene="rsmB"
                     /locus_tag="ACG8M9_000810"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0377603.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15622..16362
                     /locus_tag="ACG8M9_000811"
     CDS             15622..16362
                     /locus_tag="ACG8M9_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0377604.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16359..18428
                     /gene="pknB"
                     /locus_tag="ACG8M9_000812"
     CDS             16359..18428
                     /gene="pknB"
                     /locus_tag="ACG8M9_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0377605.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18593..19492
                     /gene="rsgA"
                     /locus_tag="ACG8M9_000813"
     CDS             18593..19492
                     /gene="rsgA"
                     /locus_tag="ACG8M9_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0377606.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19505..20155
                     /gene="rpe"
                     /locus_tag="ACG8M9_000814"
     CDS             19505..20155
                     /gene="rpe"
                     /locus_tag="ACG8M9_000814"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0377607.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20156..20794
                     /locus_tag="ACG8M9_000815"
     CDS             20156..20794
                     /locus_tag="ACG8M9_000815"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0377608.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20949..21803
                     /gene="mreC"
                     /locus_tag="ACG8M9_000816"
     CDS             20949..21803
                     /gene="mreC"
                     /locus_tag="ACG8M9_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0377609.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21807..22316
                     /gene="mreD"
                     /locus_tag="ACG8M9_000817"
     CDS             21807..22316
                     /gene="mreD"
                     /locus_tag="ACG8M9_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0377610.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22571..>23546
                     /locus_tag="ACG8M9_000818"
     CDS             22571..>23546
                     /locus_tag="ACG8M9_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0377611.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         7921 a         4245 c         5065 g         6315 t
ORIGIN      
        1 taaaaggagt tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag
       61 tatttattga atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa
      121 tctgctaaat cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga
      181 gggtatggaa ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa
      241 atttcatatg aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa
      301 gaccgtctgc ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg
      361 ctgcgtcaag ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta
      421 atgactgtag ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg
      481 ccattcgtta ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac
      541 tatgtgtctg gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa
      601 cgtggttcac gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga
      661 atgaagagct tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa
      721 tcaaaattca acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat
      781 gtagatactc aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag
      841 gttgtgaaaa agccgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
      901 cattttttca taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag
      961 aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct
     1021 catattttgc cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga
     1081 agaggttgta agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt
     1141 ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac
     1201 caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa
     1261 gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact
     1321 atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg
     1381 tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt
     1441 agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg
     1501 acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc
     1561 agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa
     1621 aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa
     1681 tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga
     1741 aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt
     1801 ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta
     1861 ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca
     1921 aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc
     1981 tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc
     2041 taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag
     2101 ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt
     2161 gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg
     2221 gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt
     2281 cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc
     2341 aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca
     2401 aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga
     2461 agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca
     2521 tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga
     2581 ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata
     2641 tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc
     2701 ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt
     2761 tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta
     2821 ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat
     2881 tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga
     2941 aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac
     3001 gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg
     3061 agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg
     3121 taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc
     3181 tggtgcaaca agaatgctga ctttggacct tcatgcagtc caaatccaag gtttctttga
     3241 tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg
     3301 aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg
     3361 taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc
     3421 aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat
     3481 cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc
     3541 tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca
     3601 aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga
     3661 agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat
     3721 caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata
     3781 aataaactca caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg
     3841 tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg
     3901 aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa
     3961 aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt
     4021 ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt
     4081 gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat
     4141 tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt
     4201 gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta
     4261 gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt
     4321 tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg
     4381 gggatgaatc cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct
     4441 attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca
     4501 gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc
     4561 tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa
     4621 gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta
     4681 gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc
     4741 acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg
     4801 atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca
     4861 ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt
     4921 aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc
     4981 tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga
     5041 agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct
     5101 ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta
     5161 cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta
     5221 tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa
     5281 tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt
     5341 gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt
     5401 aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc
     5461 cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt ttgtgatttt
     5521 tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc tacaaagtcg
     5581 aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc
     5641 gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca
     5701 agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg
     5761 ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac
     5821 tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt
     5881 tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa
     5941 ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa
     6001 cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt
     6061 aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca
     6121 tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat
     6181 tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt
     6241 gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag
     6301 acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa
     6361 gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata
     6421 tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa
     6481 aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta
     6541 tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga
     6601 tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc
     6661 agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac
     6721 actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta
     6781 tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt
     6841 taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt
     6901 agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa
     6961 cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt
     7021 tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga
     7081 tataaaaagt atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg
     7141 gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt
     7201 tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc
     7261 agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa
     7321 gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg
     7381 cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat
     7441 tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa
     7501 caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat
     7561 gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa
     7621 aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt
     7681 tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt
     7741 tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat
     7801 tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca
     7861 agtgtcaatt agtcggatgg cggaacgctt cgtgctggac gaaaacttcg taacggtaga
     7921 agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag
     7981 cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt
     8041 gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat
     8101 ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa
     8161 agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca
     8221 cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca
     8281 atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac
     8341 gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat
     8401 cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg
     8461 aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc
     8521 ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac
     8581 gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt
     8641 cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag
     8701 atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag
     8761 aaatacaata aataaacaga attggatttc ttcgcttagc ttctttgctc taggaataca
     8821 tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc
     8881 aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa atgtgagaaa ctattttcac
     8941 aaaatttttc ttcatactcg aaactaatcg gcttcttcac gacctcttat acacggcgtt
     9001 caaaagcttg catctgcgat aataacccca accaaacgaa aaacatgaga agctgtttat
     9061 gtttggctgt tcttattact tgatgcagga cagctttttt acgacctctt ggtacagggt
     9121 agctttttta ccaatttcta gctataatta aagataacct tgaaagttgc agaaaaaaag
     9181 tgcaaaataa acgataagta caaaaagact agttgaaagg acgtcgtcat gtcagagcgt
     9241 ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa aaggcacagt acgaaaagca
     9301 atttttgaga gtgaggataa tgattttcag tattcgatct caatgactac ccgtaaaatg
     9361 cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa gcaaagaaga gtttgaatca
     9421 atgatcgaag ctggagagat gctggaatac gcagaatatg tcgggaatta ttatggaact
     9481 cctttgtcat atgtcaacaa gacattggat gaaggaaaag atgttttctt ggagattgaa
     9541 gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg gtgtattcat ctttttgact
     9601 ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc gaggaacaga tgcagatgaa
     9661 gtaatcgaaa aacgaatgaa agttgcaaaa gaagaaatcg aaatgatggc tctttacgac
     9721 tatgctgttg taaatgatga agtgccgcta gcagttgaac gaatcaaaaa cattattgcc
     9781 agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata ttaaaatgtt aaaggagatg
     9841 tagtcttatg atgttaaaac cttcaattga ctcactatta gatcgtgtaa attcaaaata
     9901 ttctctagtg attttagcaa gtaaacgcgc gcatgaattg gatgcaagtg cacagcctac
     9961 tctagattct tttgattcag taaaaagcgt cggtcaagct ttagaagaaa tcgatgcagg
    10021 aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa cgtttgaaga tggaagaaga
    10081 ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg gaatcacgta tccgtgaaga
    10141 gcaaaaaatg taataaagaa aaatagagcg aacatctaaa aagaaaagca aacccagctg
    10201 tttccttttc aaacattcgc cacttattgt ctggtagaaa aggctgtgtc tgaacttttt
    10261 gacacagttc ttttttttct tatctgctgt taattttttg ttaggatttg gcagaagata
    10321 agacaaatcc atcgatctct agtaaaatag gcaaggaact ttttgtcgct tgataagaaa
    10381 agacaaaaaa aggaggaaac gaaccatgac attaacagct gaagtcattg tggacgtacc
    10441 tactatgcag accgatcagc cattcactta tttggttccc tctgaaatgg aaacagcttt
    10501 gcaagtcggg atgcgagtag aagtcccttt tggtaatggc aatcgtcatg tccaaggatt
    10561 tgttatggct atccaaaaat cagaagaatc agctaatcca tctttgaagg cagttattcg
    10621 tttgctagac cttgctccgg tggtcaatga agaattactt tcattagctg attatatgaa
    10681 aaaaattact tacgcattca aaatcacttg tctacaaacg atgcttccga gtgtgatgaa
    10741 agcagaatat gataaattga tctatccttt ggcagacaca ccagaagttc aggtattgtt
    10801 tgatcagcga gaagtgattt cctggaagga agcagaagaa gaaggcagcc tttctcaatt
    10861 gattcgctgg cgccaagagc aattagtaga tatcaaatat gaagtccata cacgaaataa
    10921 agtaaaaacg attcgtttgg tccgttcttt gttaactgaa aagcaaatcg aagaagaatg
    10981 ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt cttttgtgtt tatctgaact
    11041 gagccaagaa gaaccaattg cttatttcaa aaataaagga atcagtactg ctgtcttgaa
    11101 tcaaggaaaa gaaaagggct ggctggagtt tatagaatca gaacgttatc gcgaccctta
    11161 taaagaccga gtatttgacc aaacgacggc attggaactg aatgcagaac aaaaaaatgc
    11221 tgtcgaacaa atcatcacag caggtcagca gcaaaaagat caggtctttt tgctggaggg
    11281 aatcactggc agcgggaaaa cagaagtata tcttcaggca atcgctgatg ttttgtcaga
    11341 aaataaaacg gctattatgt tagtgcctga aatcgcattg actccgcaaa tggtcgagcg
    11401 attcaaagga cgcttcggag aatcagttgc ggtcttacat agcggacttt cccaaggtga
    11461 aaaatatgat gaatggcgca agattgagcg ggaagaagcg caagtagtag taggcgcacg
    11521 atctgctatt tttgccccgc tgaaaaatat cgggctgatc attatcgatg aagaacatga
    11581 atcaagttac aaacaagatg agacaccgcg ttatcatgcg agagatctag caatttggcg
    11641 cagtaaatat catcattgcc caatagttct aggaagcgcg acgccctctt tagaatcaag
    11701 agcacgagct caaaaaggcg tttatcagtt attgcagtta aaccaccgag ccaaagcagc
    11761 tgcacagctt ccagctattg agatcgtaga tatgcgtgaa gagtttcaga atcatcggac
    11821 ctctactttt tctgctaatt tgcaggaaaa aatacaaaat cgattagaca aaaaagaaca
    11881 aactgtccta ctgctcaatc gtcgaggata ttcgtctttt gtgatgtgta gggactgtgg
    11941 atttgttctt ccatgtccta attgcgatat ctctcttaca ctgcatatgg atacacgttc
    12001 aatgcgctgc cattattgcg gacatgaaga acctattccc aatcgctgtc ctaattgtgg
    12061 cggcaataaa atccgctact atggaacagg tactcaaaaa gtagaagagg aactccgaga
    12121 actttatccg caagctagga ttttgcgtat ggatgtagat accactcgaa gaaaaggggc
    12181 acacgaacag attttacaaa aattcggtgc aaaagaagca gatatattat tagggacaca
    12241 aatgattgct aaaggtttgg actttccaga agtgacatta gttggggtgt tgaatgctga
    12301 tacctccctg aatttaccgg attttcgttc tagtgagcat acattccaat tgcttaccca
    12361 agtttcagga cgagcagggc gagcggaaaa agcaggcgaa gtcgtgatcc aaacattcaa
    12421 tcctcaacat tacgccatag aattggctaa aaaacaaaat tatgagcagt tttatcagca
    12481 ggaaatgcat gtacggcatc gtggaggcta tcctccttat tactttactg tgaaaataac
    12541 cgccagccat cctgaagaac aagtagcggc aaagaaaatc tttcagattg ccaatcagct
    12601 aaaagaagtg ttaagccctc aaagcttgct attagggcca acgcccagca tgattttgag
    12661 agtgaaaaac cgatattatt atcaactaat catcaaatat aaacatgaac cgaatcttcc
    12721 tcatgttttg gatgaaattt tgaatggaag ccagaaagaa cagcgacaag gtctttttgt
    12781 ggcaattgac aacgaaccat taaactttat ttaagaaagg gccaaattat gcgctatcct
    12841 atattgatcc accctaatga taaattaaaa agaactgctc agccaatcga tgtaatcacg
    12901 gacgagacaa tcgctttatt agacaattta tatgagacaa tgattgcgaa tgacggaata
    12961 gggatcgctg ctcctcaagt aggacaaaac aagagaattg ctgtgatcga agtagatgaa
    13021 ggcgagaaat ttgaactgat taacccagaa atcatcgaag caaaaggtga aagccttgac
    13081 gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg tcaagcgagc agatgaagtg
    13141 accgtgcgtt attatgatcg agacggagaa gagatagaag tgacggcttt tggctattta
    13201 gcccgtgcct tccagcatga aatcgatcac ttagacggta tcttgttcat tgagaaaatg
    13261 atacagcaga tcccggaaga agaacttgaa gaatatatgg aggaacatct tgatgactaa
    13321 gctagtattt atgggcacac ctgctttttc tgtgccgatt cttgaagggc tgatagaatc
    13381 aggttacgag atccaggcag tggtaacaca gcccgaccgt ccagttgggc ggaaacgagt
    13441 aattacgccg acaccggtaa aaaaagcggc tctgaagcac ggtatccgtg tgctgcagcc
    13501 agagaaaatc tccggttcgc cagaaatgga agaaatcatt gaattggtgc ctgatgtgat
    13561 cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta ttgcaagtac caaaattagg
    13621 ggcaatcaat gtccatgctt ctttacttcc taaatatcgg ggaggtgcgc ctgttcatta
    13681 tgccatcatg aatggggaga aagaaactgg tgtaacgatc atggaaatga tcaaaaaaat
    13741 ggatgcaggc ggaatctatg cgcaagaaag cataccaatc actaaacaag atgatgtagg
    13801 gacgatgttt gaaaagctta gccttttagg aagaaagcta ttgctagaaa cattgcccaa
    13861 tattttggat ggacaaaaac cagtacctca agacgagtca gaagcaactt tttcgccaaa
    13921 catcacacgg gaacaagaag caatcgattg gcataaaact gcagaagaaa ttgacaacca
    13981 agtacgcgga atgcgtcctt ggccaattgc attcacgacc tatgagggca tacgctggaa
    14041 attattatcg gttgaagtat tggcagaaac tacagacaaa gcacctggta cgatcatccg
    14101 gaaagacaaa aaaaatctat ggattgcttg cggtaaacag accgtcttag ccatcaatga
    14161 attacagcca gctggtaaag gaaaacaggc cgtacatgat ttccttaacg gaagcggtca
    14221 gcaagttcag attggtcagc aggtgaaata atgaaaaaaa tccctaaaaa aatgaaaaaa
    14281 tcagtacgat atatggcatt actgacatta gagcgagtga acaagggtgg cgcctactcg
    14341 aatcttttgt tgaatgagat gatcaaacaa agcgagctaa atcaaaaaga tgtgggttta
    14401 tttacagaat tgatctatgg tacgattagc catcagcgac tattggaatt ttacgttgca
    14461 ccattgatcg ctaaagcaaa aaaagtggat gactgggtca agatgcttct ttatctttct
    14521 gtataccaat tagaattttt agataaagtc cctgcacatg ctattttaaa cgaggcggtc
    14581 gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat ttgtaaatgg tgtcttgagg
    14641 aattatcagc gtcaaggagc accagatctg caaggcattg ctgatcctat cgaaagactt
    14701 tctgtcaaaa tcagcctgcc tctatggatg acgaaacgac tggtggatca aatcggatac
    14761 gaagaaactg aaaagctcgg tttatcgcta tatcagccta gccatgctag tgcccgagta
    14821 gatacacgcc gactttccag agatcaggcc atcactgttt tgcaagaaga agagatcaca
    14881 gctgaaaaaa gccaagtcag tccatacggt atagtagcga aaaaagggca tttagcttct
    14941 agctcattgt tccacgatgg cgtcatgacg atccaagatg aaagttcgat gcttgtagcg
    15001 ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg cctgtgcagc acccggaggt
    15061 aagacgacgc atattgcttc ttttttagat ccgtcatgcg gtggagaagt ggtcgcttta
    15121 gatgtacacg aacacaaagt caaactcatt gaagataatg cagcacgtct aggtgtctct
    15181 tctgctgtct acgctcaaaa gatggatgct agagaagtca gtaaacagtt cgaagcagag
    15241 cagtttgatc ggattttagt agacgcgcct tgttcaggtc ttgggctgct tcggcgaaaa
    15301 ccagatattc gttataaaaa acagccggat gaattagcga atttaccaaa aatccaattg
    15361 gaaattttag aaagctgcgc accaacttta aagtcttccg gtattctgac ttatagcact
    15421 tgtacgatct taaaagaaga aaaccaagac gttgtttctg ctttcttgga aagacaccct
    15481 gattttgaga ggattgatgt tttgaccaat cctatgctac aatcatcaat cgaagagaaa
    15541 atgttgactt tgtatccgca tcagttttac acagatggat ttttcatttg ttgtttgcgc
    15601 aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat cagatgtagg aagacgacgt
    15661 aacacaaatc aagactatgc cagtgtattt accagccgtt caggtctcaa gcttgctgta
    15721 ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg ccagtcagat ggcggtaacg
    15781 aatctaggcg aagcttggga aaaacaagaa gctgttgacg atgaaaaaat cgcccaatgg
    15841 ttcattcaat cgattcaaga agaaaatatg ctgatttatc agcgtggaca agaacagccg
    15901 gaatataacg gtatgggaac aacaatcgta gcggccgctc tgtcagatga ctggttcacg
    15961 attgccaatg ttggggatag cagagcttat ttgattcgtg aagataaaat catgcaattg
    16021 acggaggatc attctttagt caatgaacta gtcaaatcag gagaaatcag cgaagaaatg
    16081 gcagtgaacc atccaaggaa aaatatttta acacgttctg tcggcatgcc tggaacagtc
    16141 gaagtagatg tttctactca tctttggcaa ctaaaagatc gactgctgtt atgttcagat
    16201 ggcttgacga acatgctgtc tgaagaagaa ataggaacga tcatcaatca agaaggccca
    16261 ttgtctcata aagtaactga gctgattgat cgtgccaatg aagcaggcgg agcggataat
    16321 atcaccgttc ttcttatcga atataaggag gacagtgtat gatcgagttg ggcaaaaaat
    16381 taaatggccg ttatcaaatc actggaaata tcggtagcgg tggtatggca aatgtatttt
    16441 tggcgcacga tttaatcttg gatcgtgatg tagcagttaa agtcttacga tttgactttc
    16501 aaaatgacca gactgcgatt cgacgatttc agagagaagc tttagcagca acagaactcg
    16561 ttcatccaaa cattgttagt gtttacgatg ttggtgaaga agacaacatg cagtatctgg
    16621 tgatggaata cgtcaaagga atggacttga aacgatatat ccaaacacat tatcctgttc
    16681 cttatgaaac agcagtaaat attatgcagc agatcctttc tgctatttct ttagctcata
    16741 accatcaaat catccaccga gatttgaaac cccaaaacgt actgatcgat aatgaaggtg
    16801 ttgtaaaaat cacggatttc gggattgcta tcgctttatc tgaaacatcg atcacgcaga
    16861 cgaatacgat gctaggatct gttcattatc tgtcccctga gcaggccaga ggaagtatgg
    16921 caacgaagca gtcagatatt tatgctttag ggatcatcct ttatgagatg ttgacgggta
    16981 gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt aaaacatttt caagatgatc
    17041 ttccatctat aaaagcactt gatccaaatg tgccacaagc actggaaaac gtgattttac
    17101 gggcaacggc taaagaacct gcagaccgtt ataagtcagc cgaggaaatg tctgatgatc
    17161 tttctacagt attatcacca gctcgtgcaa acgaagaaaa atggcagcct catgtgatgg
    17221 ataatgaaac aaaagttatc acacccctta cggaagatgc accaatgcct gattcattca
    17281 aatctatgcc tctgccaaaa gacaagccag aaaacgaaac cttaccagag gaggaagaag
    17341 ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg gctgatccta ttagctattc
    17401 ttgctgtttt aggaatcgga acagctgtat tctttgcttc tggcgggcga ggagaagttg
    17461 ttgttcctga tgtttctgat ctttctgaag cttctgctag agaaacactc gcaaaagcga
    17521 aattagaagt agcaacaaaa acagaagaaa ttgcagatga caaaatcgaa gaaggtaatg
    17581 tagtgaagac agacccggca gcgggaacga cagtcaagca aaaacgcgaa gtcactctgt
    17641 atattagtag cggcacgaaa aaaatcaaat tagatgatta cagtggtaaa agttatgaag
    17701 atgccgaagc aagtttgaaa gaattagggt tttctgctga cttgattcaa aaagaagagg
    17761 aattcagttc agaggtagaa agcggaatga tcatttcgca atctccatca gaagatacag
    17821 aagtcgatcc taaaacagac acgattactt tcacagtcag caaaggacca gaaacagtga
    17881 ctttgagtag tcatgtagga gaaaactacg aaactgttcg taattggctt gttacacaag
    17941 gattcaatat caataatatt agtgctactt atgactatag cgacactgta ccatcaggtt
    18001 cgatcatcag tcaggatcca gcctcgggtg aagttgtagc agaagaaact tacatcaatt
    18061 ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat ctccggctat acaaaaagcg
    18121 aagcacaaag ttatttagct agtgtcggtg cagaatatat cgggcatgag acttccgaat
    18181 attcaaacac ggtggataaa gataaagtga ttcgtacagt accaggggca ggaacgacaa
    18241 ttaccaaagg aatggttgtg aacgtaattt actctaaagg accagatcca gcgtcttcta
    18301 gttctgaaaa agactcttca gacagttcta gcagctcaag tagctccagt agcacaagca
    18361 actcttcaaa agaagaatct tccagctcca caagttcatc agctagcacc tcagaaagtt
    18421 caaaataagt gctgaataaa atatatcaga agggaatgcg acattgattt gttgcattct
    18481 cttttttgtg tttctatctc ttttgatcaa ttttcaagcc atatactttg agaagtttgc
    18541 atggaataac acctactttc ctcttcgtat ggtaaaataa acaaaaacgg aggtggcact
    18601 tatcaaagga caaatcagaa aagcaatcag cggtttttac tatgtatacg caaacggaga
    18661 gacctatcaa acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc cgcttgtcgg
    18721 agatcaggtg atctttgaga gtgaaaatca aacagacggc tatcttctgg aaatcttgcc
    18781 gagaaaaaat caattagttc gcccaccagt cacaaatgtt gatcttggaa tcattgtcac
    18841 aagtatcgta gagcctgcat tttcttataa tctacttgac cgctttctag ttaccttgga
    18901 atacgagtcg atcgaaccga ttattttttt gacaaagacc gacttgtcag acgacccaga
    18961 aaaaatcgaa gagatcaagc aaacctatac taaaataggc tatccggtga ttgtacccaa
    19021 tcatacgggt gatacgacag aactgatccg ttacttccca gaacggctat cggtctttat
    19081 gggtcagtct ggagcaggaa aatcgacgct actgaatcaa atatcaccgg aactgaattt
    19141 agcaacagga gagatttctg atgcacttgg aagaggacgg catacaacac gtcatgtgga
    19201 actgattcct atctatggcg ggttagtagc agatacacca ggatttagct cgattgactt
    19261 tttgacaatt gaagcaggag aactgccgag acaatttcct gaatttgtag aagcttctgt
    19321 ccattgcaaa tttagagagt gcatgcatgc taaagaacct ggctgcgaag taaaacatag
    19381 agtagaaaca ggtggtatcg cacagacacg ttacgacaat tatttgcaat tcttacaaga
    19441 aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat aggaggaaac
    19501 aagaatgaag atagcaccat ccattttgag tgcagatttt gccaatttac aaagagacat
    19561 tgagttagtg gaaaaaggag gcgcagatta catccacgta gatgtaatgg atggccgatt
    19621 tgtcccaaat atcacattag ggccaaatat tgttcaggca atccgtcctg ttactaaatt
    19681 accgttagac gtccatctga tgatcgttga tccagaaaac tacattccag catttgcaaa
    19741 agctggagca gatatcatta ctgtccacgt cgaagcaact cctcatattc atcgggcact
    19801 tcaaatgaca aaaagcttag gagttaaagc tggagtagtt atcaatccag gaacaccagt
    19861 ctccatgatc aaacatgttt tgcctttggc tgatcaagta ctagtcatga ctgtcaatcc
    19921 tgggttcggc ggacaaagct ttattgaaga aacagtagaa aaaattacgg aactcgctga
    19981 tctacgtaga gagaacaatt ggacttatgc aatcgaagta gatggcggaa tcgttccaga
    20041 aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc gttgcaggat catatatcta
    20101 tggagccgaa aatccagtcg gacaaatcga acgattaaaa gaggcattag actaaatgaa
    20161 aattttatta gctgctggag gtaatccgca aaactggccg cagtctctgc tgtccaaata
    20221 cgatgcctat gtcgggatag accgtggtgc gctgtacctg cttcaagcag gctacccagt
    20281 agatctggca gtgggtgatt ttgattcatt gtcgaacgaa gagcagaagg aagtatttgc
    20341 aaaagccaaa gagtataccc gttcccctgc agaaaaagac gatacagata cacagttagg
    20401 tatcttaaaa acatttgaac gttacccgca agcggaagtc actttgattg gagctacggg
    20461 cggaagattg gatcatcttt tagccaatct ctggttaggt atggaacctc gcttcaaacc
    20521 ctttgcccat cagcttatta tcagagattg tcaaaattat ttgtcttatt atttgcctgg
    20581 agagcatata gtaagacaga taccagatat gaagtattta gcttattgct gcttgactcc
    20641 cgtctcgaat cttacattaa aaggcagcaa gtatgtattg gaaaataaag aagtagaata
    20701 tccgacatct tatgcaagta acgaatttct ttcagatacc gcagaatttt ctttttcaga
    20761 tggtatgatt gcagttattc aaagcaaaga ttgatccata aacaaataaa aaaatttgtc
    20821 tttcaggcta aaaatgacag aatctattat ttgctaaaaa aaagcttaag aggagcaact
    20881 taccttttgt ttcacggcat ttagtgataa aattgaacac aagagtctaa ttaattcgag
    20941 gtgtctttgt gaagaagttc aatcctaata aaaatattat tattaccttg gttattgtta
    21001 tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa ggaaagccca
    21061 atttcttcca atccatcgtt aatgacagtg ttgcaatggt tgataaaaca atctctgccc
    21121 cagtacggtg ggtacgaaat ggagtagatt ctgtccataa tcttttttca acttatgccg
    21181 aaaatgagcg gctaaaagaa aaaatcgata gctatgatga cgtttcacag caaaacaaga
    21241 attacgagag agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag acgctgacta
    21301 gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc tgacagttgg caggacatgc
    21361 tgatcgtaga caagggttct aaagacggta tcgaagcaaa tatggcagtc atgtcgcaaa
    21421 aaggattgat tggccgagtg atcgaggtca atacggcttc gtctaaaatc gaattactgt
    21481 catcctctaa tgaaagctcc aatcattttc cagtacgggt atcttcggct aatggcgaag
    21541 cgtttggttt gcttaaaaac tatgatgaaa agctccatgc cttagtggtg acccaattaa
    21601 ctggtgatac ggatatcaaa gaaggggatg ttgtccagac atccggtctt ggagggaatt
    21661 ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa accagatagt tatgggctgg
    21721 atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt gtgacgattg
    21781 tccaacggct agcggaggat tagtccatgt tgaagaaaga aacaatgaag tactatctgc
    21841 caatcgtttt gttctttttg atgttgatag atggtcattt aacaagaatg ctaggggagt
    21901 ggtcgaaagg cacctatatg tcaaatgccc actttctgat attggcatta ttatgttgca
    21961 gtatggcgtt tgaaaaacgt tatttactga ttaccacgat tgttctcggg gctatctatg
    22021 acgcttacta tattggcgtt atcggtatct atgcagtagc tctcccttta attgtatggt
    22081 tgatgtatgt aatgaaagac gttatccatg tcaacatctt tactgaattt ttcagtatga
    22141 tcatctttgt cacgggttat gaattgttta cgatggtggt ccagttgatt tttaaattag
    22201 cagtagtaaa taacacgtat tttattacaa ggtttttagg acctacactg ctgttgaaca
    22261 tgattatatt tgtattattc atttttccct ttaagaaatt attcagtgat gaataatttc
    22321 tttttttatt tttaggtcga aagcgaaaaa gaatcgttgt tatcttaaga aatgctagac
    22381 ttaacgcggc tttttcatga tcattttctg aaacgaacag ttttgtcatt tttaaaagtt
    22441 gttttgaaac atttttgtaa cataactatt atatcgttga cacactgcta tggtacaatt
    22501 tttatatcgt taagaaagac ttaaatatat ttagatattt attaaattaa gccagtcgga
    22561 ggaatgaaga gtgaaaaaga gtttaatatc agcagtaatg gtatgttcaa tgaccttgac
    22621 tgccgtagca tcaccgatcg ctgccgcggc ggacgatttt gattctcaga tacaacagca
    22681 agatcaaaag attgcagact tgaaaaatca acaagcagat gcacaatcac aaatcgatgc
    22741 cttagaaagc caagtttctg aaatcaacac acaagcacaa gacctattag caaaacaaga
    22801 tacattgcgt caagaatctg cacaattagt aaaagacatt gcagatttac aagaacgtat
    22861 tgaaaaacgt gaagatacaa tccaaaaaca agcacgcgaa gcacaagtaa gcaacacaag
    22921 ctcaaactac atcgatgctg tattgaatgc tgattcatta gcagatgcta ttggacgcgt
    22981 tcaagcaatg acaacgatgg taaaagcaaa caacgacttg atggaacaac aaaaacaaga
    23041 taaaaaagct gtagaagaca aaaaagctga aaacgatgct aaactaaaag aattagctga
    23101 aaaccaagct gctttggaat ctcaaaaagg cgatctttta tctaaacaag ctgatttgaa
    23161 cgtactgaaa acttcattag ctgctgaaca agcaactgct gaagacaaga aagctgactt
    23221 gaaccgtcaa aaagcagaag ctgaagcaga gcaagctcgt attcgtgaac aacaacgtct
    23281 agcagaacaa gctcgtcaac aagctgcaca agaaaaagct gaaaaagaag cacgcgaaca
    23341 agcagaggct gaagcacaag ctactcaagc ttcatctact gcacaaagct cagcaacaga
    23401 agaatcatca gctacacaat cttcaatgac tgaagaatca tcatcagcta cacaatcttc
    23461 agcaacagaa gaatcaacaa cacctgaatc ttcaacagaa gaatcaacag cgcctgaatc
    23521 ttcagcaaca gaagaatcaa caacag
//
LOCUS       DAZKNM010000025        23297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000025 DAZKNM010000000
VERSION     DAZKNM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.025"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1663
                     /locus_tag="ACG8M9_000819"
     CDS             2..1663
                     /locus_tag="ACG8M9_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0377612.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1733..2080)
                     /locus_tag="ACG8M9_000820"
     CDS             complement(1733..2080)
                     /locus_tag="ACG8M9_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0377613.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2164..3105
                     /gene="opp3b"
                     /locus_tag="ACG8M9_000821"
     CDS             2164..3105
                     /gene="opp3b"
                     /locus_tag="ACG8M9_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0377614.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3106..4149
                     /locus_tag="ACG8M9_000822"
     CDS             3106..4149
                     /locus_tag="ACG8M9_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377615.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4155..5225
                     /locus_tag="ACG8M9_000823"
     CDS             4155..5225
                     /locus_tag="ACG8M9_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377616.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5215..6168
                     /locus_tag="ACG8M9_000824"
     CDS             5215..6168
                     /locus_tag="ACG8M9_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377617.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6180..7190
                     /locus_tag="ACG8M9_000825"
     CDS             6180..7190
                     /locus_tag="ACG8M9_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0377618.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7475..8296)
                     /locus_tag="ACG8M9_000826"
     CDS             complement(7475..8296)
                     /locus_tag="ACG8M9_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0377619.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8393..8671)
                     /locus_tag="ACG8M9_000827"
     CDS             complement(8393..8671)
                     /locus_tag="ACG8M9_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377620.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8939..10948
                     /gene="metG"
                     /locus_tag="ACG8M9_000828"
     CDS             8939..10948
                     /gene="metG"
                     /locus_tag="ACG8M9_000828"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0377621.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11427..12014)
                     /locus_tag="ACG8M9_000829"
     CDS             complement(11427..12014)
                     /locus_tag="ACG8M9_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377622.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12189..13511
                     /locus_tag="ACG8M9_000830"
     CDS             12189..13511
                     /locus_tag="ACG8M9_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0377623.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13666..14442
                     /locus_tag="ACG8M9_000831"
     CDS             13666..14442
                     /locus_tag="ACG8M9_000831"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0377624.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14435..15004
                     /gene="rnmV"
                     /locus_tag="ACG8M9_000832"
     CDS             14435..15004
                     /gene="rnmV"
                     /locus_tag="ACG8M9_000832"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0377625.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            14997..15881
                     /gene="rsmA"
                     /locus_tag="ACG8M9_000833"
     CDS             14997..15881
                     /gene="rsmA"
                     /locus_tag="ACG8M9_000833"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0377626.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16332..16961
                     /locus_tag="ACG8M9_000834"
     CDS             16332..16961
                     /locus_tag="ACG8M9_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377627.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17085..18194)
                     /locus_tag="ACG8M9_000835"
     CDS             complement(17085..18194)
                     /locus_tag="ACG8M9_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377628.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18403..18825
                     /gene="mgsA"
                     /locus_tag="ACG8M9_000836"
     CDS             18403..18825
                     /gene="mgsA"
                     /locus_tag="ACG8M9_000836"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0377629.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19012..19110
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19148..19684
                     /locus_tag="ACG8M9_000837"
     CDS             19148..19684
                     /locus_tag="ACG8M9_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0377630.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19724..21487)
                     /locus_tag="ACG8M9_000838"
     CDS             complement(19724..21487)
                     /locus_tag="ACG8M9_000838"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377631.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21490..23205)
                     /locus_tag="ACG8M9_000839"
     CDS             complement(21490..23205)
                     /locus_tag="ACG8M9_000839"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377632.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7490 a         4279 c         4753 g         6775 t
ORIGIN      
        1 aatgaaaaag aaatttacat ttggtttggt tgcggtatgc ggccttgtac ttgctggatg
       61 ttatggcggc agttcagcca caagcagtag ttcagcaaaa agcggttcag cagatggagg
      121 aggcgtattt aacttagtcg tgcctcaaga aatgcctaca gctgacttat cagttgcaac
      181 agatacaatc agttttacag cattgaataa cgtttatgaa ggaatttacc gtcttgatga
      241 agacagcaaa ccacagccgg caggagctag cgagttggca gaagtcagtg aagacggatt
      301 aacttacaaa atcaaattac gagaagacgc aaaatggtca aatggtgatc ctgttacagc
      361 agcggattac gtttatggct ggcaacgaac agtagaccca gctacagctt ctgaatatgc
      421 ttacctgtat gcgccagttg aaaatgcaga agacattaca gctggaaaga aagataaatc
      481 agaactaggg atcaaagcag ttggtgacta tgaattagaa atcaaattga cgaaacaaac
      541 accatatttc caatatctgc ttgctttccc atcatttttc ccacaaaaac aatccgtagt
      601 ggaagaaaat ggggatgcct atgcatctgc aagtgataaa gctgtttata atggaccatt
      661 cacattagaa gattttgatg gaccaggaac agatacagaa tggacttata agaaaaatga
      721 tcattactgg gacaaagata cagtaaaact ttctgaaatc aaagtcagtg tagtaaaaga
      781 atcttctact gcattgaact tattcaaaga tggccaagca gatgacgtga tcttgtctgg
      841 tgagttagca cagcaaaacg caaacgatcc agcttatact tctgttaaag aagcgcggac
      901 aagctatatc gaattcaatc aacgtgaaaa gaattcacca ttcaacaatg tgaacctaag
      961 aaaagctatt tcttattcta tcgatcgtga agctttagta aaacaagtac taggtgacgg
     1021 atcagttgtc tctacaggat tgatcccatc agatatggta aaaagtccaa caacgaacga
     1081 agactttgca aaagaagctg gaaaattagt ttcttatgac aaagataaag caaaagaata
     1141 ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta gaatttgatt tgatggcttc
     1201 tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa aactcaatcc aagaaaactt
     1261 ggatggcgta acagtcaaac caacacctgt tccattctct gtacgtcttg accgctcaac
     1321 ttcgggtgat tttgacgcag tactaggcgg atgggctgct gactatgcag acccaagcag
     1381 ctttactgat ttgtttgtca caggtaattc atacaaccgt ggtcgctgga gcaatgcaga
     1441 atatgacgaa gcagttaaag cagcttcatc aacagacgct ggcgatgaac aagcccgttg
     1501 ggatgatttc caaaaagcaa tcaagatcat ctctgatgat atgggtgtcg ttccggtgta
     1561 ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa ggtatcgttc accatgcggc
     1621 aggcgcttca tgggattaca aatggacata cgttgaagaa taatcgttaa taccgtgagc
     1681 atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt tctcatattc acttatccta
     1741 acaaagaaaa aagcatgaaa atagcagaaa gagcaattag tattccggca atgatcagcc
     1801 aaaagctgta cataccgtag ctggcagacg agagcgagga aaattctggt tttttctttc
     1861 ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg atcgaagaaa cccgaagaaa
     1921 agacccataa aaaaccgccg ataatcagaa acggcaatga ccataagaat agatcattag
     1981 ataattgaat gagtgtaaag gaatggttga tgagattttt tattaaaata accagaatca
     2041 gtagaaaagc tactgcgtag gggatagatt tttttttcat ttgattgctc ctttattttt
     2101 ggttcattat ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta gggggaaaca
     2161 ttagtgaata gttatatcaa atatgtatta aaacgtgtct ttttcatgat catcacgctt
     2221 tggttaattg caacaatcac ctttttcttg atgcaattgc tgccgggcac gccatatacg
     2281 aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt cggtttggat
     2341 aaaccagtta tcgtacaata tgggatttat ctttctaatt tgcttcaagg agattttgga
     2401 atttccttcc aatttaaaaa ccagccagtc gcacatttgt tagctggaag aatcggacca
     2461 tccttgcagt tagggttaca ggcaattatc tttggaacag tcctagggac gattttagga
     2521 acgatctctg ccatgaagca aaatacatgg gcagatacat cttctacact cgtagcgata
     2581 ttaggtcgtt ccattccaaa ctttgtattt gctgttttat tacaatatat ctttgcaatc
     2641 aaattacaag ttttgccaat cgcaaaatgg gatggctttg tgtacacaat cttaccaaca
     2701 attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac ggaaatggtg
     2761 gaagtactgc atagtgatta tgtggaatta gcaaaagcaa aaggattgag ccgttgggaa
     2821 atcgccttta aacatggtct tcgaaatagt ttgattccat tgatgacttt attaggaccg
     2881 ttagctgtag cattgatgac cggatcactg gtggtagaaa atatctttgc tattcctggt
     2941 atcggggaac aattcgttaa gtcaatcaca acaaatgatt atcctacgat catggctgta
     3001 acgatccttt actcatttat gctgattttt gttattttga tcgtggatct attgtacggt
     3061 ctagttgatc caagaattcg tgtttcagaa gggagccgag gataaatgga aatgatacca
     3121 aagcgttatg aaacagttgc agatatccct gcggatgaat ttcttccatt acaaaaaaat
     3181 accgaagaag aacgtgaaca gattactgca ccttctttga attttatcca agattcatgg
     3241 cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg ttttactatt ggtcatcatt
     3301 ttgatctctg tgatcacaat ctttgtttct cctcatgatc caactgccca aaacgtggct
     3361 tatatcaatt tgcctcctcg tattccggga atcaatatcg atggattgaa tggaaaagca
     3421 atggttggtg gtgaacttgt tgataaatat gctcaagcaa acgtcccttc aaacgtaaat
     3481 ttctttttag gaacagacgg tttgggacgt gatgtattaa gccgcttatt tatgggaatg
     3541 agaatctcgt tgctgatcgc atttattgct gcattactag atgtaacgat tggtgtagct
     3601 tacgggttgg tttctggttt gttaggcgga cgtgtcgata acgtgatgca acgttttttg
     3661 gaaatcttat caggaattcc aaacctcgtt gtcatgattt tgatgttggt cgtttttgaa
     3721 ccaggaattt tttcaatcgt ggctgctatg gccatcacaa actggatacc aatggcacgt
     3781 atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat atgttttagc tggattgaca
     3841 ttaggtgaat caagaatgaa aattgcattc aaacatattt tgccgaatat ctctagtgtc
     3901 attattattc aaatgatgtt tagtattcca tctgctattt tcttcgaagc ctttttaagc
     3961 tttatcggtt taggtcttac gcctccgtca gcttcattag gaacgatgtt gagcgaaggg
     4021 tacaagacat tcctgtatct gccttatcta ttatggattc cggcagtaac tttatcggtt
     4081 atcatgattg gctttaactt acttgctgat ggattgcgtg atgcctttga tcctaaaatg
     4141 aaagagtgaa gacgatgacg aaaattttag aagtgaaaga cttagaaatc tcttttgata
     4201 cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat aaaggagaaa
     4261 cattggcgat cgttggggaa tctggaagtg gaaaatcagt gacgaccaga agcatcatgc
     4321 gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat cctctttaaa ggtaacgata
     4381 tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg taaagaaata gcgatgattt
     4441 tccaagatcc aatgacctct ttagatccga cgatgcccat tgggaagcaa gtcgctgagt
     4501 cccttagaaa gcataataaa gtttcaaaaa aagagggaca aaaagctgca ttagaattgc
     4561 ttaaattagt tggaataccc gaagctgaga aacgtatcaa cagttatccg catcagttct
     4621 caggaggtca gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat ccggaaatac
     4681 tgattgctga tgaacctaca acagcgcttg atgtgactat tcaagcacag attttggagc
     4741 tgctgaaaga aattcagaca aaaatcgata catcaattat ctttatcact catgaccttg
     4801 gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga atcgttgaag
     4861 ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg ggactgcttg
     4921 gttcaatgcc gacattagat agtgcgaacg atcgattgta tgctattcct ggttctccac
     4981 cggatttatt gaacccgcca aaaggagacg cattctatcc aagaaatgaa ttcgcgatga
     5041 aaatcgatgc agaacaagaa ccaccatttt ttgaactatc aaaaacacac aaggcagcaa
     5101 cttggctact tgctccgcag gcacccaaag tgacaccgcc agaagaaatc caacgtcgct
     5161 gggctatctt taaagagaaa caaaaacata cttacggtca aggaggaatt gctgatgcca
     5221 aataaactac ttgaagttaa aggattgaaa cagtatttca atgtaggaaa aaagaatgaa
     5281 gtacatgctg taaatgacat cagctttcac atttatgaag gagaaacatt cggtttagtt
     5341 ggagaatctg gaagcggaaa atccactact ggccgtacga tcattcgctt gaatgaacct
     5401 actgatggtg agatattatt cgacggtcaa gatataacga agatcaaaga taaaaaaggt
     5461 ttgacgaaat tccggcatga tgttcagatg atctttcaag atccatacgc ttcgttgaat
     5521 cctcgtatga aagtcagaga tatcattgcc gaaggaattg atgttaatgg actagctaaa
     5581 tcatctgaag agcgtgcaaa aaaagtcgat gatctattga gaactgttgg attgaatcca
     5641 agccacggta ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg tattggtatt
     5701 gcacgagcat tggcagtaaa accgcgtttt atcatttgtg atgaaccaat ttctgcacta
     5761 gatgtgtcga tccaagccca agtggtcaat ttgttgcaag atctccaaaa agaacatcaa
     5821 ttgacctatc tatttattgc acatgatttg tcgatggtta aacatattag tgaccgaatc
     5881 ggtgtaatgc ataacggctt gttattagag atgggaacaa gtgatgaaat ctataatcac
     5941 ggtgttcatc cttatacaga aagtttgtta tctgctattc ctctaccaga tccagaccat
     6001 gagagaaaac gcagaagaat caaatatcaa cctgaaccag atgacggaca agtacgtcaa
     6061 cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg agcaggaagt cccatattac
     6121 gcaaaaaaac taaaacgaca aaaggaatct cttctggttg ctaactagga gggacataga
     6181 tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga acaaaaacag caattgattc
     6241 gtacttttgg atgtgctcga tttacctata accatttgct gaaaaagcgg cagaaaagtt
     6301 ggcaacaaac aggagtagcc aatttttcgc tcacacccgc tacattaaaa aaagaatacc
     6361 cattcttaaa agaagtagac agtcttgctt tagcaaatgc acagcttaat ttggatcggg
     6421 cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg aaaaaaagta
     6481 tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat ctatctggaa aatggctact
     6541 taaagttacc aaaacaaaaa gaactaatca agataaatca gcatcggcca gttgaaggat
     6601 cgattcgctc cgctacgatt tctgctcgat ataatgaaga attttatgtc gcactacttt
     6661 gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg gatcgggatc gcctatcatc
     6721 cgaagacatt gatccaaaca tctcgacctc tagaagtaac tttaccaaaa tttcaccaga
     6781 cagaagaaaa gctgcaacat gcacagcgga aattgaatgt gaaagtccgc tccgctcacc
     6841 atagaaagat caggttagat caggcaagca attatcagaa acaaaagaga aaagtaatgg
     6901 atctttatct gaaacaaaag aaccagcgag aagattatct tgaacaatta tctgggaaat
     6961 tggtcaaaca atatgactac ttatttgtag aatcttttcc aaaagaagag gcacatgctg
     7021 atttttctat tcacgactgg cataaattaa taacaaaatt gcgttataag tcccaatggt
     7081 ataataaaaa atttcttttg atcaataccg acggagcaga agaaagcaat tccgtaagaa
     7141 agagccaagt agtagaaaaa atgggcagac attcggttat caagggataa aaaatgaatg
     7201 aaactgatag gagcgttagg gttagccttg ttaataaaaa gtcttaatga cttttgtttc
     7261 taggaatttt tcttttgaag aaaagctcac taattatgac aaaccaataa aacaatagca
     7321 gaaaaagttc gtacgagctt tttctgctat tgttaatttt ttattcagaa agtttacgaa
     7381 tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg cgttttttct
     7441 tgaatgatta ggtctgaatc ttttgctcac aaccttattc atggtgcccc ggcttgagct
     7501 gatagatttt tgaaggacgg ccgattccta caggacgttc accgatctca tcgaaatatt
     7561 ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc cctaagaatt
     7621 tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt acctaaaact tgtagaactt
     7681 gcggctcgtg ttccatttta ttgactacac ggttgaatgc tttcaagatg atttgactat
     7741 ggtcaaaagc caaagtatct ttgccagaag actctccagt gactaaatca agcgagatag
     7801 ccacttctcc gctagataaa tggatcttgt tactatgacg ttctagtgtg aaccagcgta
     7861 cttccttggc atcatctccg gcgattagcg gttcttctcc aataaaggct aagtagctga
     7921 ctgtgacgac ccagcctcgc ggatctcgat taggcgtact gaatgtatgc agttgctcaa
     7981 tattttcttt tgaaataacc acaccagttt cttcctttgt ttctcttaag acgctttctc
     8041 cagttgactc atccttttgg acaaagccac caggcaatgc ccatgaattt cgataaggat
     8101 gcccttttct ttggatcaac aaaactttca gctgatcttc ttctttgtta tagcacatca
     8161 gtacgatatc gacagtcaaa gagggcttgt catattctgg acgttcctgt ttttgatacc
     8221 agttaagaaa ttcttcttca cttgctttat gttcataata taatttttct tcttctttag
     8281 aagcaaaacg gttcatctga ccacctccat tgacagtaaa agtacttttt accttattgt
     8341 agcagactct gaaaaaaaac caaggattta cttgaaaaat ctaggttatc atttaaatat
     8401 gaaaaataaa tggaagtatg agattgaata aaaagaaaag cagataaaac ggcaatactt
     8461 tataccgctc ttcacgaatg gcaaagaagc cacaaataac gatatatacg tcaatgataa
     8521 aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg cccatggagg ttaaaataac
     8581 catacatacg aatataaaaa tagctgaata ttaaatgtgc taatgaaaga aaagcaatac
     8641 taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc agtcccttac taagatagat
     8701 agtctttatt ataccacgtg tgaggggaga aataaaaagc cgttttttag tagatatgaa
     8761 acaaaacaaa aacccgcatt tattcaacct attcatcctt ttaaaggata gattgaacgt
     8821 agatagaagt cgtttatcca gtctttttca ttttgaaaaa aagctaacag catgcttttt
     8881 ttatggtata ctagcaagga aacgaattcg catcatgcga taaaggagga agagccccgt
     8941 ggcagaaaag gaaacttttt atatcactac gccaatctat tatccaagcg gcaaacttca
     9001 tatcggcaac tcgtatacta cgattgcatg tgacgcaatt gctcgttata aacggttgat
     9061 gggctttgat gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa tcgaaacaaa
     9121 agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg ctgatgtcaa
     9181 gaaattatgg aaaacgcttg atatcagtta tgataaattc attcgcacga cagatgatta
     9241 ccataaagca gctgttcaaa aaatatttga tcgtttatta gaacagggag atatttactt
     9301 aggtgaatat gaaggctggt actctgtttc tgacgaagaa ttctttacag aaacacaact
     9361 tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc caagtggaca
     9421 cgaagtagaa ttggtaaaag aagaatcata cttcttccgc atgagcaaat acgcagaccg
     9481 tctattggaa tactatgaag aacatcccga attcatccag ccagaatctc gtaaaaacga
     9541 aatgatcaac aactttatca aaccaggatt agaggatctt gcagtttcac gaacaacttt
     9601 tacttgggga gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat ggatcgatgc
     9661 attatccaat tatattactg cactaggata tggctccgaa gatgaaagtc tattcgaaaa
     9721 atactggcct gcagatgttc agatggtcgg taaagaaatc gttcgctttc atacgatcta
     9781 ttggccaatc atgctgatgg cccttgattt gcctctacct aaaaaagtat ttgggcatgg
     9841 ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg tttatcctga
     9901 aatgcttgtg gaacgttatg gtttagatgc actccgttat tatttgcttc gtgctgttcc
     9961 gtttgggtca gatggtgtct ttacgccaga agattttgtt tcccgtgtca attatgatct
    10021 ggcaaatgat ctagggaact tattgaatcg aacgattgct atgatcaaca aatattgtga
    10081 tggactcgtt cctgactatg cctcacttgt cactccattt gatagtgaac tgtctacgac
    10141 ggctgctaac gttgtcggac gttaccatga tgcaatggaa aaaatggaat tcaatacagc
    10201 attggctgaa atctgggtgc tgatttctcg tgcaaacaaa tacattgatg aaacagaacc
    10261 atgggtatta gcaaaagacg aagaaaagaa agctgaattg aatagtgtca tgatccattt
    10321 agctgaatct ttgcgtatcg cagcgatatt acttcagcca atcatgacag aaacgccaga
    10381 gaagatcttc aatcaactag gattggactc tgaaacaatg aatcttgaag gattacactt
    10441 tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct tcccacgttt
    10501 agatatggaa gaagaagttg cttttattca agaaaaaatg tctgaaggta ctcaaacaaa
    10561 cgaagataca gtcaaatggg atccagaaga aacagaactt gtttcaacga aagaaaaaca
    10621 aatcaaattt gatgtatttg aaaaagtaga actaaaagta gcagaagtca tcaactgtca
    10681 aaaagtagaa ggggcagata agttgctgca attccgacta gatgctggag acagccaaga
    10741 ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat tgatcgggaa
    10801 aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg cgtggacaaa tcagtcaagg
    10861 aatgatcctt tctgcagaag caccagatgg ctctttacaa gtaatcgaag caccaaaatc
    10921 catgccaaat ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac tagttgtgtc
    10981 ataccccctt tttccgaaat ttgagaagca attttcgtaa attccttctt attacttgaa
    11041 gccaatcata taatacagaa acagagaact atccaaaata aatcctagaa aaagaagtac
    11101 gagcttcgct gatttttgtt aatttagaat aatactaata ggaatcgtaa aaacagaaaa
    11161 aaagcataat gataaagggt cgggcagatt ttaattgatt catctggtac tcttttttat
    11221 tgtccttaca ttcataaagt ttgaatgcaa tgactttatt acatctatat ttatatctaa
    11281 cagtagcgtt gatgtgttaa ttataagttc tatcacttta tgttcaatta gtttgtgctc
    11341 ttatatgtgt gattgaaaat atagataaaa aaacaatcat cggctttgac ttcgatgatt
    11401 gttttcttta ttttgtaaaa atcctatcac ttgattttat ggtaaccttt tatttttgtc
    11461 ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa gacataccag
    11521 aaaatcgtac gtccgatacc ataatgttga tagagctttg tcaacatcac gtgtaacaag
    11581 atcaacccta aaagcaagga gccagctata aaccaatgat tatcccaatg catgacttca
    11641 ctaagtgcca tcactccaaa aggatagaaa taaaaataga tcccgactaa ggtcatccgc
    11701 cagttctttg taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag caaaataccg
    11761 ataggcggaa aaagcccaac caaaaaggaa tacaacatcc cccacaagat catcacagga
    11821 cctttgatca atgcaacgat tgcgatgaac aaagtcagca atattagttt ttgtgataac
    11881 gttgcaacag tcattaatcc aaataaaatt aatgtgaacc aatgccaatg ggactgctgt
    11941 tgttctttac gggaaaaatc gacccggttg gataaataat ctgctaattt cggataagaa
    12001 cgcttaaaat ccataaactg ttcctccttc gtggaaaagg taggtttagt atacgaaaaa
    12061 taaaaaagaa atacatccgt cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa
    12121 tggtaaatta aaatattctc agttatactg aaaattatac caaatttcaa agaagaaaga
    12181 ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca ttagctaccg
    12241 ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt actgctagta
    12301 ctcagtctac aagtttgaca ttgctagctt ggcttttggg tggtttctta acgatctgtg
    12361 ctggattgac agtggctgaa ttagctacag caatcccgga aactggtgga gctgtaaaat
    12421 atattgaagc tgcgtatgga aaattgccca gctttttatt aggatgggcg caaagcttga
    12481 tttactttcc tgctaatatt gctgcattat cgattatctt tgcgacacaa ttgacgaact
    12541 tattacaatt atctaccgat tatcttttgt tgattgcagt gatcaccgct gtttctgtga
    12601 cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg acgttgattg
    12661 ttaaattaat accaattgct gtgattgtca tatggggatt attgacccca ggacaaggaa
    12721 ctgtccagtt atttccaatc gaagctggaa aagatgttac ttttgtcgaa ggattgagca
    12781 gtgcgttgtt agcaacgttg tttgcttatg acggctggtt aggtgtcggt gcaatggcag
    12841 gggaaatgaa acggccggaa aaagatttgc ctaaagcaat tattcttggt ttgagttttg
    12901 taacagtcgt ttatctattg atcaattttg tttttttgaa aactttgccg attgatcatc
    12961 ttgccggtaa tttgaatgca gcttctgaag catcagacgt catttttgga ggaatcggtg
    13021 gaaagctggt cactattggg atcttgatct cggtatacgg tgcattgaac ggttatacac
    13081 taactgggat ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct ttcagtaaac
    13141 agctgacaaa gctttcaaag aagtttacgg ttccttatgt tcctgcggtt ttccagttag
    13201 cagtagcttg tattatgatg agccttggtt ctttcgactt tttaacagac atgttgattt
    13261 ttgtgatgtg gctattcagt ttactgattt ttatcggtgt ctttatttta agaaaaaaag
    13321 cgccggaatt gccacgtcca tataaagtac cactttatcc aatcgttccg attattgcga
    13381 ttctcggtgc tattttcatt ttaggtatga cgatgatgac gcaaacgaaa ttagcattga
    13441 tcggtatagg tgttaccttg ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct
    13501 cagaagagta aaatatttca acaacagaca gaaaaagtac gaactaggtc ctctagatct
    13561 tcgtgctttt tctatttcaa taaaacaagt tcttctattt tgatttttgc tacttctgca
    13621 agtagtgctt atgtgctaaa gtaagaatga aaaggagaga taaacatgat atttgattct
    13681 catacccatt taaatgctga acagttcaat gaagatattc cagaaacgat tcagcgagca
    13741 caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg atacgccaac gattgaaaag
    13801 tctttgcaat taagccatga ctatccaaat atatacagta ttatcggctg gcatccgaca
    13861 gaggctggca gttatacgaa agaaatcgaa aataaattac aagaacaatt gactttacca
    13921 aaggttgtag cattagggga aattggcttg gattatcatt ggatggaaga cccgaaagaa
    13981 gtccaagata aagtatttcg cagacagatt gctatcgcaa aagaaatgaa cctaccaatc
    14041 agtattcata ctagagaagc acttgaagac acgtatcaaa tcctaaaaga agaagatgtt
    14101 cgagatatcg gtggaatcat gcatagtttc agtggtgatt atgaatgggc aaaacgattt
    14161 cttgacttag ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac
    14221 gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac agatgcccct
    14281 tatctggcac cagttccttt tagaggaaag cgaaatgaac caggctatac tcgttataca
    14341 gtagaaaaga ttgctgaact tcggaatctt ccgacagaag aagtggctgc ccaaacatgg
    14401 acaaatgcgc accgattatt tggtctaagc gaacatggat aagatgaaga tccaagagat
    14461 tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg gaagtagttg atgcagatac
    14521 gatagaaaca atagggtctg caatcaatga agagatcctg atgcaaattg aacatgccca
    14581 agagactcgt ggagtgatcg tttttaccga tcctgatttt tcaggagaaa aaataagaaa
    14641 aatcattatg gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa
    14701 accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc
    14761 attgaaaaag gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa tcactagaca
    14821 agacctttta gactatggtt taatagctgg ggcacaagca aaagaaaaac gagaaaaact
    14881 aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat
    14941 gtttcgtatt acgccgaaag aactcgccca agcaatgaaa atagtggagg aacaacatga
    15001 gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga gattttattg aaacatggtt
    15061 tttcatttaa aaaaagttta ggacaaaatt ttctgactga accaaatatt ttaaggaaaa
    15121 tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat cgaagttgga ccaggaatcg
    15181 gtgcattgac ggaacaatta gccaagcatg caaaacaagt cgtagcattt gagatcgatg
    15241 atcgtttgat ccctgtattg gctgatacga tgcaacccta tgacaatgtg actattgtac
    15301 atcaagatat tttaaagacc gatttgtcaa cagctgttag agaaacattt catgaggagc
    15361 ttccgttaaa agtcgtagcg aatttacctt actatattac gacaccgatc atgatgcatt
    15421 ttcttgaatc agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg
    15481 atcgtatctc tgctgaacca ggaacgaaag cttatggttc attatcgatt gctgttcaat
    15541 attatatgga agccagttta gcattcatcg taccaaaaac agtatttgtc cctcagccaa
    15601 atgtagattc agcgattttg aaactgacaa gaagagatat accggcagtc gaagtgacag
    15661 acgaaaaagc atttttccga ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt
    15721 ggaataactt gcagcattcg tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa
    15781 gcttggaaac agctggaatc gatccaaaac gtcgtgggga gactttgtca ttacaagaat
    15841 tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga
    15901 agttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    15961 atgtttttcg tttgtttggg cttattaccg cagatgcaag cttttgaaca ccgtttaaga
    16021 aagaggttgt gggcctgccg tttaactcta agtcacaagg aacaatttca aaaaacagct
    16081 tctcatgttt ttgagttagt tggtcttatt acttgatgca agcttttgac cactattatg
    16141 ccgaacaagg agatgtgaca caaattttgt cacatctcct atttttgttt cttgatcaaa
    16201 aaattcttat acttgatact cacactcatt ttgtatggat ttagaaaaga atatgtgtct
    16261 tgacagttat tagtaagtga gttatactaa gtcgacattt atgtgtcaag acatattagg
    16321 agagagaaaa aatgagaaaa aattcagttc gaaaactaac gatttctgct ttattgattg
    16381 ggatgggggt cattatccca atggtcatgc ctaaaatcat gattccacca gcttccttta
    16441 ctttggcgtc tcatgtgcca ttatttattg cgatgttttt tacaccagga gttgcagtgg
    16501 ctgttgcgct aggtacgaca tttggtttct ttttaacaac gccggttatc atcgcattgc
    16561 gagcattgtc acatcttgtg tttgcgttga ttggggcttg gtacttgcaa aagcatccga
    16621 gtatcgtttt aaaaaatgga cagtttactt ttgctaattg gcgtttccaa gggttcaatt
    16681 ttgtgatcgg tgtgattcat tctttagcag aaatgctagt agtcagcatc ttttacttta
    16741 ttggtaatat gcctgaaaca tattattctc aaggatattt ttatacagta ttcatcttga
    16801 tggggattgg tgggcttatc cacagtttag ttgattataa tattgcttat tttatagcag
    16861 gaacactcag caaatatttt gatatccccg tatttacggc agcaaaaaaa gaaaatcgga
    16921 aagagtcgat tcaagggtac gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa
    16981 aaactctcat ctagtaatgg ataataaatc ctctaagatg agagtttttt gtcctaaaaa
    17041 gagctgcacc aacatcttct ggcacagctc taacaggggg caatttatgg aagtttgatg
    17101 acttgttctg tttctttatc aaagaagtgg cctttgctga tgttgaaggc aaggtcgact
    17161 atttcgcctg gtttatggaa atctcttgca tctacttttg agatgaactc agtagaacca
    17221 gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg aaacgacaac ttctgatttc
    17281 actacggctt caggtgaagc ttcaagggct acttgttcac tgtggatgtc ttctggacga
    17341 ataccgaaga taacttcttt tccttcataa cctttttcaa ctaatacttt gttttttcct
    17401 tcaggaatac gaagcttaag cccatgtcca tctgtgataa caccattttt caatgtcaca
    17461 ttaaagaagt tcatggcagg agatccaata aagccggcaa cgaacatatt gacgggtgta
    17521 tcataaactt ctttcggtgt accgatttgt tggataaatc cgtctttcat gataacgatt
    17581 cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa catagatggt cgttgtttca
    17641 agacgctggt gaagtttggc aatctcagca cgcatggcta cacgtaattt agcatctaaa
    17701 tttgataatg gctcatccat taagaagact tttgcatccc gaacgattgc ccgtcccaat
    17761 gccacacgtt ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa gtattctgtc
    17821 agacctaaga tctctgctgc attgtccaca cgttttttga tgtctgcttt atcatattta
    17881 cgtaatttca agccaaaagc catattatca aaaacagtca tatgaggata caatgcgtag
    17941 ttttggaaaa ccatggcaat atcacggtct ttaggtgcca cgtcattcat gacttttccg
    18001 ccgatattta gttctccttc agagatatct tccagtcccg cgatcattcg caaagtagtt
    18061 gactttccac aaccggaagg accaacaaaa acgataaatt cacggtcttt gattgataga
    18121 ttaaaatcag ttacagaata attttctgca ttgtcatatt ttttatatac gtttttcaga
    18181 gccatttcta ccataatacg ttccacactt tcttttttag ttttattaac tatttaaagt
    18241 ataaatgaaa gcgttaacta acttcaatgt catgttgaac aagaattttt tctttttttt
    18301 gtcaatgtgc tagttttaag ctttaataaa tatccataag aaataaaaac tgatacactg
    18361 gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc
    18421 gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa aacaatattg
    18481 gaaaaacatg aactttttgc taccgggacg actgggcaga ggatcacgga agcaaccggc
    18541 ttgccgatcc atcggtttaa atccggaccg ttaggcgggg atcagcaaat tggagccatg
    18601 atttcggagg atcagttaga tttggtgatt tttcttcgag acccgctggc tgcacagccc
    18661 catgaaccag atgtaacagc acttatccgt ttatctgatg tctatgagat tccgttagct
    18721 acgaatatcg gaacagctga ggtgctacta agaggactgg aagccggctt cctagatttt
    18781 cgaagaatcg tccatgaaat agacaagcat ccactttcat tttgatggat aagcgtatgg
    18841 aaaaataacg tttaatgttc gcctatatgt agctataaaa gctaaacaaa ctgataatat
    18901 gcgatataat acgaacgtat tggataatat aaacaataat attagttttt cgtatttgca
    18961 gtaaatgtga aaatatgcta tagtttctgt gggaaaagat cccaaaaata atacagagta
    19021 gaaaataaag cgttaagtgc taagaggatg ggatgttgcc tcttggacga aggacgtaat
    19081 gttcgcggtt ttattttcgc attcgctgca tgatacgatg tagcagctct taaaggagat
    19141 gctaaaaatg aagaaacacc ggttagacgc acgtatgatt gctattatgg gcttattgat
    19201 tgcattgatg gtcactttgt cccgactggt agcaatcgag accccattta ttaaaatcag
    19261 tgtcacattt attccacaag tgatcatggg cattcttttt ggtccttttt ggtcagggat
    19321 aggcgcagtt ttagctgatt tagtaggaat ggccctcttc tcgaaatctg cattctttat
    19381 tggatttaca ttgaatgcat ttattgaagg agcaatctac ggattctttt tctatcgaaa
    19441 agagatcact tggaaaaatg ccattctggc tacgcttagt gtcacgctga tcatcaactt
    19501 atttttgaca ccattatggc ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt
    19561 ttgggcgccc cgcctgttaa aaacagtaat atggttaccg attcaatcaa ttgccattta
    19621 ttacgtagga cgtagtattc cttacaaaaa aattttaaga agtttagcga tccacgctaa
    19681 ataaaaaaca agcagtcttc ccatcccaga agactgcttg tttttattgt gctgtgaatt
    19741 gtgcttgata gagccgtcgg taataaccat ctttttgaag cagctcatca tgggtgccca
    19801 tttcgacaat agctccctga tccatcacta atatctggtc cgcatttttg attgtggaca
    19861 ggcgatgggc aatcacaaaa ctggtttttc cttccatcat tgctaagaag gcttgttgga
    19921 tttttttctc tgtcaatgta tcgactgagc tggtagcttc atctaagatc aacatagggg
    19981 gattactgat catcgttcga gcaatcgtca acagttggcg ttgtccatct gatatcttta
    20041 acccttgacc gccaatctta gtatctaatt tttccggcaa acgttctaca aattcataca
    20101 tataagaggc ttttagggca tcattgattt ctgcgtcact tgcttccgga ttcccaaaac
    20161 gaagattttc tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc attccaaaac
    20221 ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg gtcatcgact gtaattgttc
    20281 cttttgttgt gtcataaaaa cgcatcagca aattaacaag tgtggatttt ccggcccctg
    20341 tttttcctac tatagcaatg gtttgacctg gataagcagt aaaattgaaa tcttcgatca
    20401 aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa agagactttt cctttgatat
    20461 ttttgggttc caaggcatag gcatctggca aatcaggctt ttcttctggc tgatcgatca
    20521 aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa
    20581 gttcgataaa tggtttagaa aactgacttg agtagatcgt aaagctggaa atcacaccaa
    20641 cagtgattga cgaaccactc ttcaatgcca ataggccgcc aactaaacca atcgataaat
    20701 aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc
    20761 gttgtccttt ttcatataga tcagcattta gcagttcaaa ttgctgttga ctagcttctt
    20821 cttgctgaaa agctttcacg attttctggt taccgattcg ttcggtcaca aagcccgaga
    20881 tatccccaat aatcctttgc tgtctatcga aatttgtttg tgaagcacga gcaacgatcc
    20941 aactcacaag gaaaataatt ggtgtagaac aaagaacgac caatgtcaga agtggactta
    21001 gacgcatcat aaaaataaac gaaagaatga tgattgccac tccagaaaat aattgattga
    21061 aggccgcaga aattgcaatg gaaatattgt ccatatcatt tgtaaatcga ctgacgatat
    21121 ttccgtggga gttttgatca taataacgaa gtggcaatcg attcagatga gcaaatgcat
    21181 cttttcttaa ttttgcaacg gataagtaag ctaaccgatt gcttaacact tggatgagcc
    21241 attgactgac tacgttgatt aaaacgatac caccgaatag aataagtaaa tggaacagtc
    21301 gattgaagcg gacctgtcct tctccaatca tttgatcgat actttgacca atttgtaacg
    21361 tcatcacaac tgttgcaatc ccattgatga ttcctagtat cacagctagc caaatgtctt
    21421 ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat aattttttta
    21481 ctttcataac tattcttcct cctgtgatgc gacgatttcc tgatattctg ctgattgagc
    21541 aagtaattct tcatgggtac cctgagcagc aatcttacct tgatccatca ctaagatatg
    21601 atgtgcttct tgaatagagc ggacacgttg ggagataata atcactgtac tgccaagata
    21661 agtctgtagc gccttacgca aatccaagtc tgtttgataa tccaacgcac ttaaagagtc
    21721 atctaaaatc agtaatgctg gcttggcaat caaagctctt gcaatcgtca gtcgctgttt
    21781 ttgtccgcca gagaaatttt tcccattttc catgactttt gtatctaaac catttggaag
    21841 ctgacggaca aattcttctg cctgagcgat tttcagagct tcccaacatt ctttttctgt
    21901 ggcgtctttc ttaccccatt gaaggttttc acgaatagtc ccggtaaata gtacagaagt
    21961 ttggggaaca agagcaattt gctgacggag atgatccagc tgccaagaac gaacatccat
    22021 tccgtccatt ataactgtac cggaactgac atcatagaaa cgcggaatca attgtgtcaa
    22081 agtcgttttc ccgcttccgg ttggaccaat gattccaagg actgtatttc gcttcagctg
    22141 aaatgtaata ttttggagag aaagaccaga atcaggagta taacggaagt caacatggtc
    22201 aaaagcgata attgcttcag aagaaaaatc gggagtagta ctgacctcag cttcaagaat
    22261 cgaattttct gtgtctagca cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa
    22321 gatcacgacg aggtttgaaa caacgatcag tgccaaaagc atttgattca tataattgat
    22381 caaagcaaga acttgtcctt gttgcaagct accaatattt actttgatcc cgccaactgt
    22441 cagcaaacag atgatcccga cattcatgat caaggttgta gcaggtgata atagggcaga
    22501 gatattggaa acacgtaaat aattgttcgc tagatcattt gtgcttttat cgaacttctc
    22561 tgtttctttt tttgtctgcg caaatgcgcg gatgacacga acaccgctaa gattctgact
    22621 aacgtgttca ttgagctgat ctagtttttc ttgcactttt tggtagagag gaacggtttt
    22681 tttgatgata aaaaataaaa tgattgagaa aataggcaat aatgctaaga aaaacagacc
    22741 tacttcccag tcgatcacaa aagccatgac cactgacccg atacttaaaa acggtgctct
    22801 gatcaataaa cgaatgacca ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat
    22861 tcgggtgatc aaggtatctg ttccaaaatg attcaactcg ttgtgagaga aagtattgat
    22921 ttttttgatt agctgattac gtaactctgt accaaatcct tgtgaagcta ctgaagcata
    22981 gtattggcaa atgatagcac aaatcagacc aatcacagac atagcgacca tccataagcc
    23041 catttcaatg atttttcctc ggtcattcat ttttagtcca ttatcgatca aggaagccat
    23101 cattaaaggc aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat
    23161 gatttgctta cgatagtctt ttgcataacg taataactga atcatatgat tcctcctatt
    23221 atttatttgt ccacaattga aaaaacaagt actaccacat tgtagcttag gaaatttaga
    23281 atgaaaagag aacaagc
//
LOCUS       DAZKNM010000026        23001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000026 DAZKNM010000000
VERSION     DAZKNM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.026"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..478)
                     /locus_tag="ACG8M9_000840"
     CDS             complement(<1..478)
                     /locus_tag="ACG8M9_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0377633.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQL"
     gene            complement(499..1590)
                     /locus_tag="ACG8M9_000841"
     CDS             complement(499..1590)
                     /locus_tag="ACG8M9_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0377634.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(1598..3808)
                     /locus_tag="ACG8M9_000842"
     CDS             complement(1598..3808)
                     /locus_tag="ACG8M9_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0377635.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(3824..6352)
                     /locus_tag="ACG8M9_000843"
     CDS             complement(3824..6352)
                     /locus_tag="ACG8M9_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0377636.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(6404..6814)
                     /locus_tag="ACG8M9_000844"
     CDS             complement(6404..6814)
                     /locus_tag="ACG8M9_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0377637.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(6827..7045)
                     /locus_tag="ACG8M9_000845"
     CDS             complement(6827..7045)
                     /locus_tag="ACG8M9_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0377638.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7042..8034)
                     /locus_tag="ACG8M9_000846"
     CDS             complement(7042..8034)
                     /locus_tag="ACG8M9_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0377639.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8113..9024)
                     /locus_tag="ACG8M9_000847"
     CDS             complement(8113..9024)
                     /locus_tag="ACG8M9_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0377640.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9043..9387)
                     /locus_tag="ACG8M9_000848"
     CDS             complement(9043..9387)
                     /locus_tag="ACG8M9_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377641.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(9407..9685)
                     /locus_tag="ACG8M9_000849"
     CDS             complement(9407..9685)
                     /locus_tag="ACG8M9_000849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377642.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10044..10544)
                     /locus_tag="ACG8M9_000850"
     CDS             complement(10044..10544)
                     /locus_tag="ACG8M9_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377643.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(10545..11090)
                     /locus_tag="ACG8M9_000851"
     CDS             complement(10545..11090)
                     /locus_tag="ACG8M9_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377644.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11087..12343)
                     /locus_tag="ACG8M9_000852"
     CDS             complement(11087..12343)
                     /locus_tag="ACG8M9_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0377645.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(12704..13015)
                     /locus_tag="ACG8M9_000853"
     CDS             complement(12704..13015)
                     /locus_tag="ACG8M9_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377646.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13038..14513)
                     /locus_tag="ACG8M9_000854"
     CDS             complement(13038..14513)
                     /locus_tag="ACG8M9_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0377647.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(14962..15414)
                     /locus_tag="ACG8M9_000855"
     CDS             complement(14962..15414)
                     /locus_tag="ACG8M9_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377648.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(15416..15781)
                     /locus_tag="ACG8M9_000856"
     CDS             complement(15416..15781)
                     /locus_tag="ACG8M9_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377649.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(15880..20163)
                     /locus_tag="ACG8M9_000857"
     CDS             complement(15880..20163)
                     /locus_tag="ACG8M9_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0377650.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(20310..20549)
                     /locus_tag="ACG8M9_000858"
     CDS             complement(20310..20549)
                     /locus_tag="ACG8M9_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377651.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(20639..20881)
                     /locus_tag="ACG8M9_000859"
                     /pseudo
     CDS             complement(20639..20881)
                     /locus_tag="ACG8M9_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20994..21245)
                     /locus_tag="ACG8M9_000860"
     CDS             complement(20994..21245)
                     /locus_tag="ACG8M9_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377652.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            21408..22367
                     /locus_tag="ACG8M9_000861"
     CDS             21408..22367
                     /locus_tag="ACG8M9_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0377653.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            22505..>23001
                     /locus_tag="ACG8M9_000862"
     CDS             22505..>23001
                     /locus_tag="ACG8M9_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0377654.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         6846 a         4925 c         3509 g         7721 t
ORIGIN      
        1 tcaattgaag taatacaaat tgagtaacct tattcattgt ttcttctcca aataacaaat
       61 attgatccgg attctctata tcttgtgtca cctggtaaag tttcccagtt gttttcattt
      121 gtacatgcgg gtctacatca atactattgt ctttaataca acgatctgtc atatagtccc
      181 tcacagcttg attgcgttta taaacatctg tgaaattact ccattttcta gcaaaatctt
      241 ttaagaaatt ttttgtcatt tcatctattt gcatttgttg cgctgtctcg tcttctaatg
      301 aaccaatttg actgttactt tcagtacttt gaagagttcc acttgtttct tttactgctt
      361 cgattgtttc ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac
      421 aaccagtaag acctatcaaa cataaccaac taaatactaa aattatccct tttttcacgt
      481 ttctagtcac tgcctttctt atttcacctt acgaaccaga ctacgaattt ttgtttgatt
      541 gtacgtgcgt tgataccgag ccataatccg tccttgctcg gcattctgtt ccacactaga
      601 gaaatgttgt ccgccatatg aaacatctgt tataattccg gtgtgaccat agattccagg
      661 agacaatgca ccacctgctt gccaattcac cacatctcct gcttgtaaat cacttggttt
      721 tgggttcatg attacttgcc aaccataggt tgtccagtta taatcttccc ctattaacat
      781 cgcaaacatg tgtccagaac ccattaactg tgggccacct aattgagata cataccatgc
      841 cgtaccacca taacattccc cattatttac ggatcgtcca atgatccgat ccaaaacctt
      901 cgcattgcct ttactttttc ctgcaataag cgaagttttt tttgttggta ggctattcca
      961 atgcttgatc gcaaaagtat catattccgt taagttatag gacgttatga tactcattgc
     1021 tcctcgataa tacggactcc cttcggtaat acctgctgcc gcatagcctc cacgacctag
     1081 aatcaataaa caacttccag gatctttatt gcccacacat tgattaaagt tgctttggct
     1141 cgccaccata aagccataat agtcataaca agcttttaag ttcggaaact tcatatagaa
     1201 accaccttca gaaccaccaa ttccacgtgc agaaccttgt gggtagggac aaccaggaaa
     1261 ccaggtaatc ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc cataagctga
     1321 ttcatagcct aattgagaaa gaatcacact cggtaataac tgatacttta cagctgcacc
     1381 aactgctgct tgcgcatgtt catctgtgat tggaccacgt cctttaatct ctgaaaaaat
     1441 cggtaatcct tcggtatatt ccccattaaa agcgacagct agacctgtag cttctcctcc
     1501 atgctcagaa agttctcctg aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa
     1561 aagaagacaa cctagacacc ctttcttcat ttcttcctca tcctttcttc gataatggtt
     1621 tttggactgt tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg
     1681 gcagtgtttt aggatccttt tctatttttt ccgattgctc tgttggctta tctaaagaag
     1741 aaaatgcagg ttgtaacgat tttggtgcgg aaatcgacgg tttactttca gacgaacgtt
     1801 gccccggttg ttgcacagaa cgatttcctg atagagtggc taccgacgaa ttaggaacag
     1861 agtattggtt caataactgc tgcattgctg gataactccc atttttcgcc tgatttttca
     1921 cttggcgaat ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg ggtacttttc
     1981 cagaaggcaa cggaatcgat tgaccaacaa aagcgatacc tttatttaac aatggttttc
     2041 tttgattatt cccttgttgc ttactcaatt gtgccaatga ttgtacctgt tgttttggtc
     2101 ttactgaatt catggacgaa tttggaatca aaccatttga aagaccagaa ttccactctg
     2161 ttttcgattt ggtagaagac atttcaggct ctaccccatg ataacgaaca tgacgaccac
     2221 tataaggcaa ggcttgattt gaacgcccac ctagttcaaa gggattaacc acttgcctcc
     2281 cttttaccga ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg
     2341 cagaagcgag tggattcgtt gctcgataca cattccctgc taatcctgca cctttgattg
     2401 ccaaattttg tccagcagtt gatagttgac caggcagttg tttcatttga gacaaagaat
     2461 gacttgctat gtttttcgtg ttgtttatag tttgatctgc agctcctaac cctagtttct
     2521 ttaataaatc tttccgtttt acaatgagta aaattgatag gagtgctttg acaaacatat
     2581 ttcctaaaaa accaaaaaca gtattcattg gaattaaatc gtctactatg ttatagatca
     2641 aaaatgccac caaaataatc acactgtaaa aagatttttg aaacatcaag cccaaaaagg
     2701 actttaaggt attcatgagc atataatcaa atactggaac aaaagaagcg agtaatgaaa
     2761 aaggaatcac aagtaataaa cacaacacca acatttgaaa gataaaacga atcatgccaa
     2821 tcactacaag tggtaatcca atacttagat taagcaatgg caccatagaa ccaacgactg
     2881 ctttataaac agctttagtt ggttgaatat agacacgata agccttcttt ttccctctat
     2941 cttctcctgc agcagtggtc acttttgtct caatatcaga taattcttca cttgacgctt
     3001 ccttggctag aaattcttgt ggttcaatct ctgcttgata caattcttct aaatttgttg
     3061 tcccataatt taacaatata tacggatcaa caacagccgc tttataataa agttggcgca
     3121 ctttttgaat ggcttccttt tgtgtcgttg gcactaaaac accattgaca tcttttgtcg
     3181 ccaattcatc tagactattt gtagcagaaa aaataaagcc ttctacctcc acggacaaat
     3241 catttaaccc tttggtaaaa gcttctcctt tactaggtac gttactacca tagccaaacc
     3301 aaaccattga aaaaatgaca acgaagagaa atcgaataaa caccattttt gcgtaatgtt
     3361 tccctctaaa tatacgaaga gtaaatagat ataacacata caagacgaca atccccgcac
     3421 caataggatc gtatattttt gaataaatag tatccgaaaa agtgaaaaac tggtgaatta
     3481 actcgttcat tgcagctcct tcatagagct tctcaatgac aaaatcaagg ccttgccaaa
     3541 tcaatttact taaactaaag aacatattcg tcatcatttt tagaaaatct gtgccatttg
     3601 ccccaaataa cattttgcct aatgaaaatt cactatcaga taaataagaa gaaaaggtat
     3661 caatatgaaa tttcccaatc tcatagatcg attgatccac ctgcttttct gccgcataca
     3721 ccaatccacc aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt
     3781 tcttttcctt atgctttata taagccattg tttcacctca tttctattgc atgattaacg
     3841 tatcctgttc ttctacttga gagtgacttg ttttttccgt tgtttgtaat gcagcggtca
     3901 tttcttcaaa caaactgtgt acattgagtt ttcctacacg tccataggga tctaacatcc
     3961 aacactgtcc tttaatcatg tggctcattt cagctaagtt cgcttcattt acttccaaac
     4021 gtaaatgatt caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa
     4081 aattcccttt ttctttatct gaacctaagt caccaataaa ttgtgtatct aacactaatt
     4141 gattcatctg ggaacgcccc acacgtgcca ttgctgcaat aatactacgt cctgcgttcg
     4201 aattattaaa tacccatgct tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt
     4261 ttccaaacat ttcacaaaaa cgacctaaac taatcatcaa acacaaagac tttcgatccg
     4321 catctgtata tagttctggt cggacattgt catttggtaa tttcaaatct tggatctcta
     4381 aaatcgttgt tttggcatta aagtctaatc ccgggttttc tccataagaa aatcctaaac
     4441 gtaaaatcga atcttctacc gtcaagcgta aaaactcacc atattctcga acctcttttt
     4501 gttccatttg ttctaaatag tcgaccacat gcatcaagcc aacttgttgt ccggcttctt
     4561 tttgaactaa aacttcttgg attcccttta ataccgcccc ttttaaaagg tgattggtac
     4621 ctaacggact taactcatca atcatatcct gggctacttg tttcgcatct actcgggata
     4681 aacaaacaat tggatctaat acaccataat tacggggatt tgtagcatct aacgtgacat
     4741 aactcatttt ttgcaaatgt tctacaaaca aagggtaatg tttctgataa tacgggtctt
     4801 gaatgagttt cgtaaaccat ttgcgctttt ctcctttggg atccacaaat aaactttgta
     4861 cattcataaa cgaactatac aaaaagagca agccaactaa aaaggtcttc cctttaccgg
     4921 tttttccagt tactgctaca tgaggcgcat caaacaacgc acctttgatt ccttccgcaa
     4981 tggctagaag attcaaaaag acaaaacgat ttgacgcata aattaattct tctaatggtg
     5041 ttttttctcc tcgagaacca ccatccccta atgtatcttg ccgaccaata taccagccgg
     5101 attccatacc taattctgat actgctgaaa acaaccattc gccaactgct tctacagttg
     5161 ttcggtgctt ccaatgtaac tgccgtccca atggttgccc taataaacat ttatagaaca
     5221 attctcgttg atcatttttt gcacggtgga tatggataat cgtatgaagt tgttgaataa
     5281 ccgttgcact attttgacga acttcttcaa ccgtttgacc gtatacaata aaacatgcga
     5341 tccaatcgac gacaggttgc ccattatcta ctcgttgctt taaatgtttt actaatcctc
     5401 gaacagttaa taagcgttca ctaggttctt catcattttg aaccaattcc ttatgctctg
     5461 ttttgaaccg ttccgatagc caattcagtt ttaaaccaat acctccctgt cctttcaagc
     5521 gtggatagtg aattttcatt cgcaattctg ctgggaaaga aagatcctga gcaaattgaa
     5581 atagatcaca atttctcata ttttcaggtg tttccccaat aggcaacaat gctaaataac
     5641 tactccctaa ctcatcttct aattgaagaa ggcctttatg ttccttcggt atacgtacat
     5701 acgttccttg attgtctttc agtacttccc aattattttg ttcttctact ttgtgagcca
     5761 ttcctcgaat atattgcaga cgattgagat acaacagctc tttttttact aagggacgaa
     5821 tagaaaagct ataaagtaat tgtgtcactt cttttacata aggatcactt tcagaaaaca
     5881 aagtttccaa atcatctact tctaatccaa ataagcgtgc taaacttcct gctaaatcta
     5941 accagattag atttattaca tcatgaaaac tctccaccgt tgctttttct ttgactttga
     6001 ttcctaaaat aaatgaagga cgatagactt gaccttctcg ttccaacaat gtcactgttt
     6061 cttttgcata atacgcacca attgcttgac tatcgggaga aaatcccttt cctaatgtgt
     6121 caaacttcgt ccgtaaattc attgttcgtg gatacatcac caactccaca tcttcataat
     6181 ctgctaaaag caacagtaaa cgagccatca tttcttttat ttcctgtgtc ttttttggtg
     6241 tgttttttga aattgttcgt ggaaaaacat gatagtaggc ccagatacaa tcctctccat
     6301 ttaacaaaca atttttcttg aatgctttta ctggttcttt tacaagcacc aatgaatttc
     6361 acctcccaat aaaaaaggaa caaccagttg attgtccctt tttctaatcc tctaaataaa
     6421 catacttatt cacaaaaagt aattcttcca atgcattgtt tgataccttg tcccagttat
     6481 aatacacaca atcaccattc atcatacgaa tcatataaca aaaccaatcc cataaataaa
     6541 aatgcacttt ttttccatca ggttttagtt ttgccagtgc taacacacat aaatagggga
     6601 gtcctatata aaaaacaact cctgctggtg gaaaaaaaga aatgacccaa ccaataaatg
     6661 aacgtaaaaa taaatattca aagattacga ccaacaaaaa agttgctgca ttgactaatg
     6721 caactggaaa tggaagacgc caattgcttg taatctgttg tacgcgataa ggttccgcat
     6781 aaacaatcga ataatcataa ggttcccaat ccacttcact atcctcctaa tccataaata
     6841 atttttcaat gattttacca aacgcatcaa atacacgtgt tggatcattt aagaagaaat
     6901 aaactgcggc acctgctgcg atagatatta atgcatttcc tacttttgat ttcataaaat
     6961 ttcgcaatcc cagataaaca accaccatta ttaaaaaata gccaccttgc gttaatataa
     7021 aatctacaat tcccttcagt gtcatttccg ttcttctcct ttcctatcca accaattatg
     7081 tgttaatttt tcaacataat actgatttgt ttttttcgta atgacaatcg tcatttgttc
     7141 tttttgtaaa agtgacgtat caccatctcg aaagacgacc gttgctttta caataaaagg
     7201 ttttttcgtt tctttataac gcaatacttt catctctact tgatcaactt tagctgtttg
     7261 attgagtcct tcaggttctt tcatcatgta tgacatatct tttgcattgt ttttcgcata
     7321 cttatctaag aaatcttcca caaatccttc aacatctttc gcttccgacg catcaaccgt
     7381 ttcgtataat gtgtcatcat aagtaatcat tcgttgtttt tctgtattct ttggtagtgc
     7441 cgtaaaataa ggataagcaa taatacgaca gcccttgttt gaaatagaaa agggaagatt
     7501 gagtacttgt gtgattgtca cacttgtttc tttcttactc tctggattca tcgagcgaat
     7561 atcataagtt actttataac tagcagttgg aatggtttca atttctttta atccaataaa
     7621 cgaagcagca gttaattttc gaacacctga aacacttcct ggttcttgaa taaagttttg
     7681 tgcataaaac tcttttttta atgtttcttg acgttgttgt tcttgttcat gattatcgaa
     7741 tgtattcata tacaaagcaa caaaacgatt cataaaggat tcaatttcag gtgtgtacgt
     7801 taattctgac cgactttgta cttctttctt ttgtaattgt tggatttcat ctttcaaaaa
     7861 cgacacttga accttcgttt tcatggattg aaaaaatgcc aagccccctg aaaaacaaag
     7921 aaataaaacc aaacaccaaa tccatttcac tgcttttttt gaatggattt tcttttgctt
     7981 cggtactttc tctgtataaa ctttttcttt ccgccgtagt tgtcgttttt tcataagcta
     8041 ctccttctag tatgttttta ctagtacgta cgattgacaa cgataagctt gtttttctga
     8101 aaaatagtcc attcaattac cctcattttc tattcctttt ctctttcttt tatatccaac
     8161 aaatccagca atacttgtca acactcctcc tagaacagaa agtgcaacat gtatttcttc
     8221 accagtcttt ggtaagactt ttgtttttcc atctgctgtt ttgatttcaa tattgccact
     8281 tggaagtttc tttacaggaa tcgacaaatc ctttaccaaa ttcccttcac tatcttggat
     8341 taatggaata ccatctttta caccaacaat tgtttgatca cctgctactt ttaatggggt
     8401 ggttggtgtt ggaatatggg tcaattcctc tacattacta gtgacaatcg gtacactcat
     8461 tccttgagaa gtattcgtcg taatttcacc gcttggggtt accgatactg tgacttcttt
     8521 tgctggaaca ataggtttat tttcttttgg aattgcttct gatttttcaa caggttgttt
     8581 cggttcttct ttttgttctt cactggtaga cgacatcgtt ggctgttccg tacttgacga
     8641 atcagtattc ttttcagaag aactggttgt tcctgattgt tccggagttg tgactatact
     8701 tgaatccgtt gttgttgaac tatgcacacc ttcttcttcg atcgaatcag tcggtaaagt
     8761 tgattcagga aactctggtt ctggtgtcac tggctctttt tcaggtttct ctgactgcaa
     8821 ttgttcaggt tctgctggtt tttccgttgt tcctccattt agctctgtct caacatctgg
     8881 taatccttcc cctggcaatg tttctacaat ttctgactta ggtgtttcta cagtttcatc
     8941 tgcataaacc aatgaactcc ctaattgaaa cgaaaataat gaaattccta gtgttgttac
     9001 gactgctaat gttgtttttt tcatgattat tcctccaatt ttttatgcaa ttcgtgtttt
     9061 gttcatagac gccgcactaa tattatctga acgataaaag aactcccgtc cgttttcttc
     9121 taacccaaca tatggattat ttttgaataa ttgaatcaat agttcactgg tgttgtattt
     9181 tggatagttt gcaaaaaaat aatactgtgc ttgtcgccca tccgaaaatt caacaagtaa
     9241 ttcataacga taccgcttca atccttttcg ttgttttaaa ggatctctat accaacttgt
     9301 ccatcgttcc attcgtggat agagatagac acgatacaca tagaacaatg ccataccaca
     9361 taccaccaaa aataataatc gccacataaa aattccccct tttcttctac ctttcttgaa
     9421 tcatcgaaca ttcataccct ctatagagat ttgaaaaatc catcatcaat tgttcatata
     9481 aacgttgttg caaatagcga ttgtttcgaa tacataattc ttcatagcca tcaatcactt
     9541 gatcttcttt ttcatagcat cttgtagctc tatgacgctc agcttgcatg acataaccaa
     9601 actctgcacc atatcgttct actgttaata actgttgact acgcaataat gcaatataac
     9661 aatcacaaaa atgttcaacc gacatttgat ttttctcacg tttgtgaaat aggtattctt
     9721 tagaaagcac attattcgtt aaacaatctg ataaatagcg ataatctggc ggtcggttat
     9781 tttcatctgt gaatttttcc cataataaac tgatttttat tcgttcagcg gctagatagt
     9841 cttgttcgcg tggataaaat tcatcaatcg cacaaagttt tttatcacct gaataaacaa
     9901 atccatataa tgcgaaacga actcttcctt ttcaattggc tctcctaaaa attccagtga
     9961 cataccatct tctccttcct aaaaaaaata aaaaagcaga aaccgatttg gcttctactt
    10021 ttctttcctt ttatagctct tttttacgga agaatccgca aatgacattt tttcaaaaaa
    10081 tcaatcggca caagttcacg aaaacttgga gcaattttgg atagaatcac ttccccatct
    10141 tgtaaaatac aatcataaaa atcatcagtt acttgatact tacgcttctt acaataattg
    10201 ataaaatagt agcgatcatt ttctatttcc attcgttgtt ctactaagcg catacgaaca
    10261 tcatcataga aataagccac aaatcgccct tcaagttcat gtttttcaat gggatactct
    10321 tctggtaaca atctattttt tcgtcgttta tacattgctg gataccttag aagaagttca
    10381 tctaaacgtc tgtcagtata tggtaactga cgaatcaacg attctaatcg ttctttatac
    10441 agatagaata attggcgcaa gcaatagtgt accgcctctg gattttctaa tactgttgga
    10501 tcacgtgacc aatacgcacc aacataaatc ataatttttt tcatttagac aatcacacca
    10561 gcaatactaa tgaccgatcc acaataggta gaacaagtgg catagcgacc aatttcctct
    10621 aattctatct caatctcttc taaagaaaga tatgtctgtt cttctgttgg ctggcgactc
    10681 atttcccaag tgacagtcac caatgaaatt tcttccataa gaggtttttc gctgctaagt
    10741 gctgccacac agttttcata tatcttcatt ttatcgcagc cttttttaac cgctaaaatc
    10801 gcaggtattg gcaatgaaaa tcgataaaag cgaatgaatt ctagccttcc tttccttcgg
    10861 ctaaattcca actcttcgag ttcttgttcg tatcccttac gaacttctaa taagcgtgca
    10921 atttcttgcc agttctcttt ttcctgataa cgtccagctt ttaccgcttg ttcaatataa
    10981 ggaactcgaa aacgaaaata agacaaatca accatctttt catttaatgc ttcgattgca
    11041 gcctttaaat ccaacaggcg cagccattct gttgtcgtat atgcattcat gcaattttcc
    11101 ctcctgacgt tcccatccat tctagatagt tttgttgctc gtcatttagt tcggcattat
    11161 caatcccttc ttgtacaaag tctcttccta aaatacgatc tgcttcttgt aacaacttta
    11221 atgtcggagc tacttgtttc aaaaaccagc gttctgaacg atctaatgag aatttttctg
    11281 gctttactgt aaaatccaat tcttccatcg aacgaaataa ttttgcccat cgtttatcaa
    11341 caatccgtgc atcactttcc agtctatccc aaacaataag ttttgcatta attaaaccaa
    11401 cgccaatctc tcctaaacgt ttcggtgaat cacctaaaat aaatgcttct actgcttgtg
    11461 ttgctttctc attggctaac cgtaactcat atcgattata aataccatat ttcgttaatg
    11521 cttctaattc tgaaattttt tctcggttcg caatttcaaa acgtttctca taaaaattaa
    11581 aaaagagagg actctttcgc gatccaaaat acaaagataa tccttcactt cgaaaatagc
    11641 catcatctaa aaaacaaccg ccaatattct ggaatctctt aaagtccatt tctaataaac
    11701 ctgctttttg tttttctagt aattcaaaga gatcgaaatg ttcttccgtt tccaatcccc
    11761 aaagttcatc taacgcaata tcgaatcggc tacattgcac acgtgtataa ccagcttcca
    11821 actttccttg atttttccaa atggaatata gccatgttcg ccaatctgtt tgttgttcac
    11881 gcatgacttc ctcatattcc cggcaacctt gtcctgaaaa ttgcacaagt acattttctg
    11941 ttacagttcg ttttgaatgc cagaacaccc agatattccc acgaacagaa agacagtcaa
    12001 aaagatacaa gcccgttgga cgtgtttcaa attctgtcag atccattcgt aatacacgtt
    12061 gaatcactgt tttgtagtct gtcccaaaaa aggaaagacg caaataatca atcattgctc
    12121 gaagatctat tccgcttttc atatacaatc gaaagcgttc cttgattcgt tgttcaaaat
    12181 cctctgggat ttttcttttc ccttgttcca tctttctaat gtaatctttt gagcagccca
    12241 tcaattgcgc aaatcttgct tgtgatagcg gctttttatc tccacttata ttatgaattg
    12301 cacttaactt ctttctgtag ctttttagct caatcggtgt cattttctcg ctccttttat
    12361 aaaataacaa aatcgatttt ttttaaccct ttagaaacac cgatatatca actagtcccc
    12421 ctcgtttttt gggaaaagta gtacaactgc tgaccttaga taatcacttg atacaaaagg
    12481 gtttataaaa cttttttcta tgtgttatgt tatttctgcc ccccatatta tatatcgggg
    12541 ggctttacac aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg cacacgctgc
    12601 tagcgagtgc gcattatagg cccatgcttc gcagccttaa tgcgcaccgc caggcagagt
    12661 gcggctacca gactacgtct agagccctcc cgatagataa taactacaaa attaaatgat
    12721 ctaattttgt tatttcacta tatttttcag gagaaaaaac gcgataaatt gacaaagtta
    12781 aatcctcttc taaatctact cgataatgat acaatcgagt caatcgttct atatcttttt
    12841 tcgttagcaa tacttgatta ataacttttc ctgtaaaaac acgttctttg cgcaaaaagc
    12901 ggtaatcagt tacttgttca atacgattca accaatcata aaacgttctg gaagaaagag
    12961 aaggattaat cattcgtatt gctgtagcta ctctataata acaactctct gccatttaac
    13021 ttcacctcct tagttgatta caaaatatgc gcatcagaaa aacttgttcc ttttggatct
    13081 tttgctaaat acatttggcg ctctagttgt tcttgtaaca actgattatc atctcccatc
    13141 gtttgatctt gactatcctc ttttactgta aaatcataga caatcatttc agtaaatttc
    13201 tcaaattctt tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa ctctacaggt
    13261 acatcaaatc ctggaaaagc atatccacga ccaggttgat ctttgttgta aaatggctta
    13321 tttttaaaat cttctccaaa agtcattaaa taaccaatgg gagacaatcg accaacagaa
    13381 atacggaatg ttaaattatc ccgaacacca cttggaaaat attctgcatc tggtcgttgc
    13441 attgctaaaa taatgtacat ccctgcttga cgacccttta atactaatgg ctgaatcaat
    13501 ttatcgactc tatttctcgc ttccatatcc aacgaaccat aaaaagctgc ccattcatca
    13561 aaaaagataa aatgcggtgg taacccataa taagcataat ttttccctgg ttcatattta
    13621 ggatctttca tggttttcat ataggcatac cgttgattca tttcttttaa tgcatttcgt
    13681 aaacaaataa tcatttcttc atttattttg taaaacacat gtcctttgaa tacatcgacg
    13741 tcacctaatg ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt cccaacactt
    13801 aaacaagctt gaattaaaga atacaataaa taggttttcc caccaccgat tgcaccagca
    13861 atcaacatat ttggtgattg atcatatacc caatccaaag ttttcattaa atgaatacgt
    13921 ccattttctg ctatacattg atcaatagga atccgaccag aatctgaaag gaatacataa
    13981 ttgattcggg ctttttctcg ttcttcacga attaaatcac gcaaaaatac ttcagataac
    14041 aaatgaccca tttttaaaaa tcgttcgtga aaacgcccac catctaaagg aaaagaaacc
    14101 actaattctt gtttcttttg ataaaaataa acttttggta atctcatttt ttcccttgtt
    14161 tgtttctcac taacttgttt ctttgtataa acaaaccgat tgtcaattaa cattcgaaca
    14221 agtaactgac gccaataaac agaggcaaaa tatccttctt ttagtcgcga ccaatgcatc
    14281 aaccctaaaa ttaacccact aaccagcaac aaatacaaca caatacttat tccaggaata
    14341 agatacattt gccaattcga aaacgtactt aaacgtggat aaaatacttt ccaactaaac
    14401 caacctaaca gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa ccataaataa
    14461 cgattgaaat agcgaattcg ccgaccacga tacttgaaaa aatgagcttt caattgtcac
    14521 attcctcctt tctgttttta cttttcctgt gctaaaaact tttgacaaaa agcaactaat
    14581 acaggaatat cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg atttttataa
    14641 gggtgtgcac acatattaca tattcgtact atacgaatcc aaatcacttc ctgatgatct
    14701 tgtttccact ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat acactgccca
    14761 ataaccgcac aaatatatgc atttgtttgt acaaaaatcg ttggattgtc tccaaaatac
    14821 tcaatcaatt gttggatatt ttgataacaa gcacaatctc tcaaagtatt tgattggttt
    14881 tatctgaatt caaaatctcc ccttccttct atcttagata aaaagaatag gaagactcct
    14941 tcctattctt tcaaaaaaac cttatttttt ctctttttcc ttagaatcaa cacttggttg
    15001 tttttgatct aaaccttttt cttgtgattg tttcatatta ggattatttg ttggagcttc
    15061 tactttctct aatcctttcg cataaatcac taaatctgaa tattcacgat tgccatcttg
    15121 tgctggaata tataccacat gatattctgg atcaattaat ttcacaggat cacgtcgttt
    15181 aaaaccagtc aaatctacat ctgctggaaa tttaactcgt cgtgcaatct ttggttcatc
    15241 tggatttcca aaaactaatt gtgctcgctg tgatcgaatt tctcccgtat aatcgcctgt
    15301 ttcattatca aatacacgat cagactcttc taatgcgcca aagcgtacgg ctcctaaaca
    15361 agtttcatct aatgcttctg taatgtcata attatttgga aattttaatc ccatcttaca
    15421 aaacctcccc aatttttaca ccagttaaaa agacaccaac atcttgatag ccagttgttc
    15481 gacgctctgt ttgtcccata tcatttgttt cacgactatt cactcgttct ttacgaaaaa
    15541 caaaataagc ttcttcaaat gaaatacgca cctctttttc tggtaatcgt aatcttttat
    15601 cctcttcagc tgtgagataa acaaaaaaag caggctgttc ttctcgcgaa cactctgctt
    15661 gtaattgaac atatcgccca gacacaccat taatttcctt tggtggatta ggttcccgtg
    15721 aacccaataa aaatacttga cctaaaaact ctttcttcat acgcaatgct ttttctgcca
    15781 taaagttttt cctcctctta aaaaccaaaa taaattctaa atatttctac attacaatca
    15841 aacaagcggt taaaaatcct aaccgcttgt tcctcatgac taatctttgg ctttacgtac
    15901 tttataaagt gttataccta aagcaatgac taatactact aatccaagaa ccataagtat
    15961 actacttgtt tgttcacctg tttttggcaa cattggtaca acagtagctg gtttaacccg
    16021 ttgttctggt ttcactggtt gcggtggttt tggcgcttcc acaatttgaa ccgtttgatc
    16081 tttgtttgtt agatctacat tgtgtttcgc cacttcttca aacgtttctt tctcatcttc
    16141 cgttttcgct tggagccatt ccgcaaaact caagcctttt cctgcaagat tgcgaccgtc
    16201 cactaagaat tcaaataaga attccatcat ttctttatcg gccgcaaaga ctttttccgc
    16261 atgagtgcct tcaaatggtg tgccgtctgg tgttaagtag ccttgaacaa ttaagcgata
    16321 gatttgacca atcgcaagac cgtctacttt gacccattca agcacagttt gatttggcaa
    16381 ggccaagatc tcttttccgc catttttatt cgtcgctttc gtcgtaatgg ttggattact
    16441 gattttgatg gtttggtcat tgtcggtaat atctgcgtga gaagccaata atacgccttc
    16501 tctaaacaga tcttcaaaga ccacgacttc tttgcctcgc aacgcacgtg cgtctagaat
    16561 aaagtctaga ttgactgtac catccgttgt ttctgccaca aacgttaagg tacttgtgac
    16621 ttcttttcca tctactagca ctggtttttc cgttactttg tccattagaa caccgttgac
    16681 tgtgtatgtt tttccaacga ccaaatcgtg gtatttcaca gcgtctgtaa tggtgacttt
    16741 cgttaatgga ttaacctctt tcaagccagt gggtttatgt gtagccgttg tacgtacact
    16801 tgggttagtt actcgtaccg tttgcccttt gtcgttgatg tctttatggt ttgctagttc
    16861 ttcatcattt ctaaaaagac tttcaaacac gacgatttca cgaccattta tacctgtcaa
    16921 atcaaaggta aatggtacga tcaccgtttc attttctgct ttggcaataa agccaacact
    16981 cgtttctacc gtatttccat gcacaagcaa gggttcaccg gttgctttat ccattaagat
    17041 ccctttgacc aagtatgctt ttcctggaag aatctcggta atttcaacgg tatcgtttaa
    17101 ggtaaacaat cctgttgggt ctgcgactgt caatccgtct tcaaagacag ccgtggtatg
    17161 aacggttgga ttattcactt tcacactttg ggcttcatcg ttgatatccg catgtgctgc
    17221 aacttcaatg ccatcacgga aaagtttttc aaaaacaacg atcgtttgac ctttcagaac
    17281 actagcatta accacaaatt caaggtcttt tgtgccattg actttgtctg caataaaggc
    17341 caaggtactt gtgatttctt tgccatcttg taaaattggc gcattcgttg ccttgttcat
    17401 caatactcct gaaatggtgt attcttttcc gacaaacaag ttggtataag aaacactgtc
    17461 tttaatgacg atcgtttcta atggatcaac cactttttca ccatcggctt tatttgacgc
    17521 ttgtgtttct agttttggtg gaatcaagta attacgtaca gaaaccacta acgtttcccc
    17581 atcatctggc gcattttgtt tgaatgcaac cgtgaatgct tgtttatctg tatttggttg
    17641 gaaactttct aatggggcat tttccaagaa gtaatagtca tcggcaacta gacctttcac
    17701 taaaatttcc ccgttttgat cagtggtata ttcaccaatt gcttgatttt taccgttata
    17761 caaggtaaag ttcacacctg cgagcggcgt tttcttcttg ttccattctt catacttgaa
    17821 taattttaca cttttccgtg cttgacggtt cgcaaaatgg atcactttga tcggttctgt
    17881 atcattggta atcgagaaca ctaatgggtt ttgattcaaa acgtaattta cgggggcttt
    17941 gatttcttcc aaacgatagc gatcttttcc ataagtaagt gtaccgttag ttaggaaaaa
    18001 gcctttttca ttggttttaa agatatccgt tgtttctgta tcatttggaa tctccattgt
    18061 gacataacga ttggctaaag tatcaaaaat cttgaattct gtattagcaa gtggaatcac
    18121 ttttcctgtt tcttgatcca ctttcaccac tttaagacgt tgttcaatta ctttatcttc
    18181 taaaacataa tggaaagttt gcccattttc agaaattgtg acttcaagtg gttcaaatag
    18241 tttataccct tcttttcctt tggtttcaga aagaatgtac gtatcaaaga caacggtatt
    18301 tttaccaaaa gtagcataac catcattgtc tgtcacaacg gttttcacca ctttgccagt
    18361 tgtttttgac gtcaaagtga tttcaatgcc agctaatttt ggtttgttat ctggattcgt
    18421 tgaatcgact aaatctttat tagcaacttt cactaaatca atggttcctt tgatcacttg
    18481 atcttttgcg gtcgcattga cagtttttcc aggttcaaca acaatttcaa ttgcttcatc
    18541 agaaagcaca taaccttttg gcgctttgat ttcttttaaa acatatttgc caggatcaag
    18601 atctttaatt tctgcctgta ctttgccgtt aatttttttc aaggtaattt ctttaatctt
    18661 tttcccatct gcttgataca aaccatagac cgcattttct aaagaagctg caccttgtgc
    18721 tttcacacca gtttccgcat cttctttaaa cacacgaatg tttccttgtt ttagaatttg
    18781 taaattaata acagacgcat taggttctgt tggatgtaac acaccaattg tttgagctcc
    18841 tggacgacga taggctaatg gcgcaccttg aactttaatt ttattcaatt gaatttttgc
    18901 tttatccggt gcctcttttg tgactgtaat ccatacttca ttgccgtgaa tagatacatt
    18961 gacacctttt ggtttttgaa tgtctttcac atatttcaaa aaatcatttt tattctttac
    19021 tttcaacgtt tcgccaattt ttaccgtatg tgtttgatta ttaaaagttg ctttttcacc
    19081 aaagttatta atcaaacgtt gaatttctgc ttttcgacga tcataatcaa cacggatatt
    19141 tttgattggg atttttgtgc ccaaagcttc ccaaatcata aattgtgccg caatgtattg
    19201 ttcatcggat ttgtctttat ttgtgacata tcctaaataa gagatcaagc ttgttttttt
    19261 ccgtacattt gaatttttca aatagttttt caaagcagta gctgtataaa cttgaccttc
    19321 tgcggctgag acaccaggct caatacaaaa cgccacttgt ccattaatct caaaacgaac
    19381 catataattt aaagtgaagt ttagattgtt cggactagaa gtaacaccaa tattttcgcc
    19441 ccaatcaatt ttccgaccac ctaaaacagc tgcattggtt gcacggcgaa cacgaaccga
    19501 acgattggtt ggtactgtta ctaacagtgt aggtgtacct gtttttggaa tgttctgctc
    19561 ttttttcttt tgattttcaa tactagccgt agattctttg tttggtttcg ttggttgatt
    19621 ttgactagct gcggttgaag agattgtttt ttcaacgttt tcctcatttt ttccttcttt
    19681 gtcagcagta gccgctggtt gttccactgt ttcttctatt cgtttctttt cttccgcttg
    19741 tttctcagaa gactctggct gcacagaagg cgttgcttcg gcagcttttt ctccttctag
    19801 agtggacgtt tctgaagcag gggttgtaat tgaggcagtt tcttcaactg gttgtgaagt
    19861 accaggacta ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag tcgttttgga
    19921 attgaccgtt ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac cagctgggac
    19981 cccggcttct gctggttgga cttctgtcac ttcagaagcg acttgggcaa tggcttccat
    20041 cgtaggttgt atagccgtcg tttcttctgc atgtgcttga gcagttccca ccaagaaaca
    20101 gaattgagaa aaagccacca atgcgagtgc tttcaaccaa ttcattttcg tttttgcata
    20161 cacaagaatt cctccttttc ctgagaattt tatataataa aacaaaacgc cgttaccaaa
    20221 agtgataacg acgtgaactg tctcttctat ttttatctgg cgctcttatc tgaacacatt
    20281 tcatcaatga ttttaccctc ccactataat tacagaacaa aaaaagaacc tgcttcgtgg
    20341 aaatcaactg tccatttccc aattggaata ccagcttttt tggcaacttc accatcacca
    20401 aatctcatgc cataccaatt actaccttca tccttaattt ctttccaacc atatgacata
    20461 gcttcatcta atgaatcaaa aatcatgaca ctattgccaa ttgttacagg atctggctta
    20521 agatcttctg gaagagaaat atcaatcata ccataatgat ccgattcctc agtattattg
    20581 tcattactag ctactggttt ggttgttcta ctggcttatt ttgatacaac aagtactctt
    20641 attcttcttt tacttctccg ttttcaattt cccattcaaa ccactccgca aagtcttgct
    20701 taaaaccatc tactcgatac ctattttgta aataggcatc ttctgacgtc gtaggatcta
    20761 aataggtagg tttattctct ggacgagcat agacacctac ccatgtttcg taatctcctt
    20821 caaaataact atctaccaaa tagccttttt ttctaagaat ctcccgaact tgatttttaa
    20881 ttattgatac ttgttcaaaa ccatcactca ttctatcacc tcaaataact ctagattctc
    20941 ataaatattc ccgacaattt caatcctatc aatgatctct aacaaatcgc tacttatgct
    21001 tccccattcg tataagaatt tcccttccgc aaaataaatt ttccataaac ttcttcatga
    21061 tcgtcccaac caatatcact ctcgaaaaat tccacgttgt tcttatcttt tagctcagta
    21121 aatgccataa gttcaacatg ttttccaacc tcgaaacaat cacaccaccc atcaaccgtt
    21181 acacgtgtgc cttcaatacc taagaaactt tgtcctcgtg tattaaacac taaaactttc
    21241 cgcatttttt gctcattaat taatcacgcg cgaaatctag gaatcacaac ttattcgctt
    21301 cttctttata aatcgccgat tgtaaaatta agctagacaa ctaaaaaaag tcatttgtgc
    21361 tacactcaaa atagttccga cgaaagaatt ataaggagtg agtacaaatg acctataacc
    21421 atcttacacc aactgaactc gtaatgatag aagcttattt caatcaatca caacctgttt
    21481 caaaagtagc caatctgtta caacgttctc gacaaactat ttataaggtc tatcgttttc
    21541 ttaaatctgg tggaagcgca atgagctact ataaacaata taagaaaaac aaacgtaact
    21601 gcggaagaca tccgattgtt ttacctgatg agcaacagga atacattcaa aaaaaggttg
    21661 ttcaagggtg ggctcctgat gtccttattg gtcgagcaga gtttcctatt gcttgttcgg
    21721 tacgcacact gtatcgaatg tttaaagata aaacctttga cactcacgat ttaccaatga
    21781 agggaaaaag aaaaccaaac ggtcataaag aaaaaagagg aaaacaaggg tttcgccggt
    21841 caatccgaga cagaaagaca gactataccc agtttgatca tgaatttggt cacctggaag
    21901 gtgacactat cgtgggtgga aaacataaaa gcgcggtcat cacattagtt gaacggctat
    21961 ctaaagtcat cattaccttg aaacccgaag gcagacaggc taaagacatt gaaaataggc
    22021 tgaatcaatg gtttcgttgt gtcccaaaaa acttatttaa atccatcaca tttgattgtg
    22081 gaaaagaatt ctcaaattgg aaaaagatta gtaataccaa cgatatttca atttattttg
    22141 cggatccagg aacaccttct caacgaggat tgaatgaaca ttcaaatggc ttattacgta
    22201 aagatggttt gtataaagct atggatttta atggtgtatc tgaaaccttt atccaatcgg
    22261 tggcatcgaa acgaaatcat attcctagaa aatcattaca ctatcggaca ccaatggaag
    22321 tatttttgag ttacgtcaca aaagatgatt tgtctaactt aatttgacaa acgaaaatta
    22381 caaaagtagt gtttcaagag agtataaaat attttgtgta aatgaaaaaa tccatacaaa
    22441 aaaggaagtc ccttctgtag aataaagtta acgacaacca attcacagaa aagaggactt
    22501 ccctatgaat gattttacta cagaaattgt gcaaactcta gtcactaaag gcgatttaaa
    22561 tgaattattc cgttcgcact tagaaaaagc gataaacaca ctcctacgga ctgaattaac
    22621 ggctttttta gattacgaaa aatatgatcg cactggtttt aattcaggta attcgagaaa
    22681 cggttcttac tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg aaatacctag
    22741 agatcgtaat ggtgagttta aacaacaaac tttaccagcc tacaaaagaa caaacgatac
    22801 attggaaacc actattatcc atttattcga aaaaggtgtt acgatgtctg aaattgctga
    22861 tttgatcgaa aaaatgtacg gtcatcacta tactccacaa accatgtcca acatgactaa
    22921 agttctgact gaagaagtaa atgcctttaa atccagagcc ttaaatgata agtatgtcgc
    22981 tatttttatg gacgctactt a
//
LOCUS       DAZKNM010000027        22919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000027 DAZKNM010000000
VERSION     DAZKNM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.027"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..376
                     /locus_tag="ACG8M9_000863"
     CDS             212..376
                     /locus_tag="ACG8M9_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377655.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            373..603
                     /locus_tag="ACG8M9_000864"
     CDS             373..603
                     /locus_tag="ACG8M9_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377656.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            788..1333
                     /locus_tag="ACG8M9_000865"
     CDS             788..1333
                     /locus_tag="ACG8M9_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0377657.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1430..1651
                     /locus_tag="ACG8M9_000866"
     CDS             1430..1651
                     /locus_tag="ACG8M9_000866"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0377658.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1808..1972)
                     /locus_tag="ACG8M9_000867"
     CDS             complement(1808..1972)
                     /locus_tag="ACG8M9_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0377659.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2074..2595
                     /locus_tag="ACG8M9_000868"
     CDS             2074..2595
                     /locus_tag="ACG8M9_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0377660.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2582..3022
                     /locus_tag="ACG8M9_000869"
                     /pseudo
     CDS             2582..3022
                     /locus_tag="ACG8M9_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3062..3220)
                     /locus_tag="ACG8M9_000870"
     CDS             complement(3062..3220)
                     /locus_tag="ACG8M9_000870"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0377661.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3313..4074
                     /locus_tag="ACG8M9_000871"
     CDS             3313..4074
                     /locus_tag="ACG8M9_000871"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0377662.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4261..5613
                     /locus_tag="ACG8M9_000872"
                     /pseudo
     CDS             4261..5613
                     /locus_tag="ACG8M9_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5629..6849
                     /locus_tag="ACG8M9_000873"
     CDS             5629..6849
                     /locus_tag="ACG8M9_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0377663.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            6884..7561
                     /locus_tag="ACG8M9_000874"
     CDS             6884..7561
                     /locus_tag="ACG8M9_000874"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0377664.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7558..8751
                     /locus_tag="ACG8M9_000875"
     CDS             7558..8751
                     /locus_tag="ACG8M9_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0377665.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8766..9092
                     /locus_tag="ACG8M9_000876"
     CDS             8766..9092
                     /locus_tag="ACG8M9_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0377666.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9096..9431
                     /locus_tag="ACG8M9_000877"
     CDS             9096..9431
                     /locus_tag="ACG8M9_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0377667.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9428..9823
                     /locus_tag="ACG8M9_000878"
     CDS             9428..9823
                     /locus_tag="ACG8M9_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0377668.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9820..10191
                     /locus_tag="ACG8M9_000879"
     CDS             9820..10191
                     /locus_tag="ACG8M9_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377669.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10207..10767
                     /locus_tag="ACG8M9_000880"
     CDS             10207..10767
                     /locus_tag="ACG8M9_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0377670.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            10892..11203
                     /locus_tag="ACG8M9_000881"
     CDS             10892..11203
                     /locus_tag="ACG8M9_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377671.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11227..11418
                     /locus_tag="ACG8M9_000882"
     CDS             11227..11418
                     /locus_tag="ACG8M9_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377672.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11418..15266
                     /locus_tag="ACG8M9_000883"
     CDS             11418..15266
                     /locus_tag="ACG8M9_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0377673.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15267..16139
                     /locus_tag="ACG8M9_000884"
     CDS             15267..16139
                     /locus_tag="ACG8M9_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0377674.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16136..17185
                     /locus_tag="ACG8M9_000885"
     CDS             16136..17185
                     /locus_tag="ACG8M9_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377675.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17189..18013
                     /locus_tag="ACG8M9_000886"
     CDS             17189..18013
                     /locus_tag="ACG8M9_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377676.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18031..20091
                     /locus_tag="ACG8M9_000887"
     CDS             18031..20091
                     /locus_tag="ACG8M9_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0377677.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20108..20257
                     /locus_tag="ACG8M9_000888"
     CDS             20108..20257
                     /locus_tag="ACG8M9_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377678.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20254..20700
                     /locus_tag="ACG8M9_000889"
     CDS             20254..20700
                     /locus_tag="ACG8M9_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377679.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20702..20839
                     /locus_tag="ACG8M9_000890"
     CDS             20702..20839
                     /locus_tag="ACG8M9_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0377680.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            20874..21116
                     /locus_tag="ACG8M9_000891"
     CDS             20874..21116
                     /locus_tag="ACG8M9_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0377681.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21132..21329
                     /locus_tag="ACG8M9_000892"
     CDS             21132..21329
                     /locus_tag="ACG8M9_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0377682.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21340..22356
                     /locus_tag="ACG8M9_000893"
     CDS             21340..22356
                     /locus_tag="ACG8M9_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0377683.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22471..>22919
                     /locus_tag="ACG8M9_000894"
     CDS             22471..>22919
                     /locus_tag="ACG8M9_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377684.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNT"
BASE COUNT         7972 a         3895 c         5082 g         5970 t
ORIGIN      
        1 ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca actatgcaat gttcgattca
       61 ttgccagtga ctttggcaac cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt
      121 cgtacgcgtt ttgtgcatcg tgtaggttgc tattacatat tagatcactc gttgagtggt
      181 ctttttattt tacataaagg aggtaacaac gatgtataga ccacaatact tagaacagaa
      241 gtatgaagta atcactgtgc aaaacggtaa cggtgagata gtacgaaagt atagaagacc
      301 aataaagagc gatacatata aacgaaagga aaacaatgaa gttatttctt ttcgcagaag
      361 gagaaaagcc aaatgagaaa ctactggtat gtatcgctaa ctaatgaata tcctcgaacc
      421 attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa tcaaagggaa gtactctatt
      481 gtcgaaatgc taagagaagc tacaccaaac gaagtggata aatgcaagct gatatattgc
      541 ggtcatggct attggaaaga cgagtatatc caacaaaaca ttgagaggtg gatagataga
      601 tgagttacct cgaacatttg aaacgttgct acatgcattc taagaataaa cttcctgaca
      661 gctacactgt agatgatgtt gctatacatg tattgaagac tgagagccat agcagtcctg
      721 atggtagcag taaggagcag acgctcgcgt ggttcaagtt ctttaagtgg attaaggagg
      781 aagagtaatg cacatgcaca taggagaaaa gatatataaa ttgagacggc ttaataacga
      841 aacaatgaac gacttagtta aacaaactgg agtgtcaaaa tcatggttat ctgatattga
      901 gtctggaaaa aagcaacgcg ttgattttaa taagatatgt gcaatagcca aacattataa
      961 agttagctta gaatactttc gggatgatta tttggatagt gatgaacacg aggatattat
     1021 tcctttgaca ggcgaaacaa gagttaaatc agtgaaatcg cttgaacaaa gcctaaagta
     1081 tctgaataac aaacaagaac ctaaccttct agagatcaaa ctaaaagata ctgactcagt
     1141 accagaggtt tggtacaagg gtgagaggtt ggatgaattg cctaaaggat tagtagatgt
     1201 ctcgtatcat tggaagactg atgattttac taatgatgat agaggagcga acgacatcac
     1261 gattcaatac ttttctagct ttaatgataa gtatccagat aagaaaacaa tcggacacaa
     1321 gagagatatg taaatgaaag aagttagacc tagagacgaa atagacaaac tatacaagac
     1381 caaacgatgg cgagacctaa ggcaagtagt aatagctagg gacttcggca tgtgccaaga
     1441 gtgcaagcgt cgagggcgga acacaagggg cacgatcatc catcacatag tcgaggcgag
     1501 ggaagacctg tcactgttct ggtccgtaga taaccttgaa tgtatctgtg tagcttgtca
     1561 caacagagag catccagaga gatcaggcgg gaagaagaaa ccaaaaccta aatcacatat
     1621 cgttaaaatg tattcaactc ctgaaagata agtttgcagc gaaatgaagg tagcccccct
     1681 actctaaaag attaaagagt aaggcttgag aagaacggtg ctgtccttcc ttcgtaaaaa
     1741 gaccgctttt caagtttttt ggagaaaaag gaaaaagccg accaatttaa gccggctttg
     1801 gacgaagcta tttcttagtc catttgtttc ctttttgaga agtagggggt aatcggtcgc
     1861 ctggatcaat ttttacttct tgtccgcctt ggacatttcc accacgagga cccacttctt
     1921 tgtaggttcc ttttggtttg ttgtcttcgc cgggtttata gagttctccc ataggaatcc
     1981 ctccttaaca aatttcggca cagcactgcc gataacctaa ttataaggat tgtgataacg
     2041 atttcaatct atcttttgaa agaaggtgat attatgccgc aaccagcgaa gagtgcaaaa
     2101 ttacaattat taaacggaaa cccaaataag aagaataccg aagaactccg caagcgagcg
     2161 gccgcagaag acaaattaaa aatggctact gacaaaatca aaccgccgtt atggctagat
     2221 tcgctaggaa aggatacctt tgagtttatc gccgatgaat tgctgtctgt ggatttaatc
     2281 agtaatccgg acgtccatac aatggctcta tactccaatt ggtattcgca atacgtttct
     2341 ttagaaaaac agcttcgaaa actacaacga gagtacaagt tgaactatgc gcttgcgaaa
     2401 aaggaggcag aggcgagagg tgagccgttt aatgaaccta atgaattaat tggtaatccg
     2461 ctctctcggc agatggatac agcgtctcgg aatctccgtt cttttggcgc tgatttagga
     2521 ctatcaccag cagccagagc taagttagct attaagatgg ctgatgatgg tggtgatgac
     2581 gatgacgact tctaatattt tggatatgtc ttacacagaa cgtgtggact attggcaaag
     2641 ctatcttgag gagcaagctt cttggggtgg ctttttaaaa tgtccatatc cggaattgtt
     2701 aactacttgg tatgcggaac gattaatcga tggaagcata ccagccagca aagaaaatat
     2761 tcaagctgct aaacggcata tgcgtgattt gcagcgccaa ggaacagatg attttccttg
     2821 gatctttgac gaagaaaaag gtcaccggcc tattagatat atcgaaaaaa aatgtaaacc
     2881 aactgaaggc gactttggtt cgtttgtttt gcaaccttgg cagcatttca taattggatc
     2941 catgtacgga tgggtacatc gtgatacagg agagcgtcgc ttccgcgagg ctcttatttt
     3001 tgttggacgt aaaaacggta agttttgccg tttaaaaatc gggcaaaatc ggtgaaaacc
     3061 tttatttttg gctttctttt agtaataatt cgatagcttt atcaagcaat ttagacatag
     3121 gaaccatagt ttcttccgac atttttttta atccttcgta taaatcttta tcaattgcgt
     3181 tagaaattct aattctgttt tttagtccgt atttgtccat gtgtgacact cctttttatt
     3241 ttactataac atccgattcc accgcttgca actaccatca attgatggta gtatataaaa
     3301 gaggtgagta gattgaaaaa atacattatt tacaaacact taaataaaat aaatggcaaa
     3361 atttatatcg gtgtcacaaa tgatattggc agaaggtgga ggagcggtgg gattgaatat
     3421 aaaccaccaa agaacgaaac ccaacaccac agaagttttt ggaatgcaat acaaaaatac
     3481 gggtgggaaa attttgatca tttgattatt gaagaagact taacgatgaa agaagctttt
     3541 gagaaagaaa agttttatat tgaactttat gactcaacta ataagaaaaa aggttataac
     3601 atcgcaaaag gcggaaatgg cggaataata tataaagttc accccaaagg aatgctaggg
     3661 aaaaaacaat ctaaagaatt ttcttcaaat cacagtaaat gggcaaagaa ccataagaat
     3721 aattgtatga caaacggtga tgttgtatgg ggtgttactc acgaacaccc aaaaggcatg
     3781 ttgggaaagc accacagcaa agaatcaatt atgaaaaaga aagcatatag tggtgaaaat
     3841 gctgtaacct caaaaccaat tgtcgcaatt gaacgggatg gaactaaaag agaatttcac
     3901 agcgctaagc tttgcatggc ttactattcg attagtacat gtgtttttta ccgactttta
     3961 aaagatggcg ccccttatgt gataaatcct aaagcgaact acagaaataa agaaaaaata
     4021 ttagctatcg aaggaattat gttcaaacat aaagaagata ccgaggtaag ttagtagatt
     4081 gcgaaaggtt actaactacc gtaacgcgta ggagttgaat aaatataatg ctcccaagag
     4141 tgtccgacag cagataattt gctgaaaatg tacgctaaac tgggccagaa ctgactgacc
     4201 gatgaaaatg agggtgacct ccagagtgcg agataaaaag ctcgcagata ataacaatcg
     4261 aaaacatcac taatttcagg cctttccaca tacatggtcg cttatgatga tgaacaaggc
     4321 gccaacgttt acgtattggc aaatgctcgt gatcaagcaa gcttgttgtt tgataaggcc
     4381 gcagaaatgg tcaaacaatc gccggcgctc tttaagaaat ttggtaagcc taaacgatca
     4441 agtattaatt atgctcccgc cttttctaaa atggaaccac gcgcctcaga tagccggaaa
     4501 ttggatgggc taaacactca ctttggtatt tttgacgaga tccacgagtt tacgaattac
     4561 aatctgatca acgttatcaa gaaatcaaga ggaaccagaa aacagcctct gatagtttat
     4621 atcacaactg ctggatatgt attagatggt ccgttgatgt cttattttga gcaaggtgtg
     4681 gactgtttgg aacatttgga agatgacatc gatgaacgga ctttctatta tctggcaaaa
     4741 cttgacagtg cggaagaggc tgatgaccca agattatgga tcaaagccaa tccgaatatt
     4801 tgtctaatga attttgttgg catgctagat gactatgtta aggataaaaa agatccaaaa
     4861 gaatatgctg actggattac caagcaattt aacttgtttt ccgatatcga tgagctgtca
     4921 tttgtcgata tgcctaccat taaacgaaac aataaaacca tcgatattga aacgctcaaa
     4981 ggtaagaagt gtgtcggtgg ttttgacttg tccgaaacgg aagactttac cgcagccgtt
     5041 ttagaatttc cgcttgaaac aggcgaggta ttcattttgc aacacacatg gatcccacaa
     5101 gctagatttg atcgagataa caatcaagag cgtatcaaag cgtgggagaa ggtgggagat
     5161 ctaacgatta ttcctggtga ttacgtcaat tatgaatacg tcttaaattg gtttgtagaa
     5221 aattcgaaaa tctatgacat tgtaaaaatc aattatgaca aggccaaggc gctacgatta
     5281 aataaagaac tagaaaatgc aggatttgaa accaacgaga ttcggcaagg gtttctatca
     5341 ttaggtgggc caatgcaaaa cttcaaggaa atgctattgg acggtaaggt gattttcaac
     5401 aattccaagc tttaccgatg gtatctatcc aacgtcaagc tggtgatgga tcgcaactca
     5461 aactggatgc cgtctaagca gtccaagagt agaaaaatag atggttttgc agcaagtttg
     5521 aacagccacg ccgaagtgtt gaatatgttg gttaatcctg tcggaaccgg gaaagtaacc
     5581 tattactcga tttccgattt aatgaatatg taagaaaggt gtggaggaat gagtatttta
     5641 gatcgtttgc gttcttttgg ccgagcgaag ccgaaagcga gcaaacaaga gtattttttg
     5701 aatgacccgg gattgatacc gtatttagtc ggaaaagatg aaatatcaga agggattttt
     5761 tccgtaatta gccgtgtatc gaacgttttt gcgtctctcc ctctcaaaat gatagatgtg
     5821 gagtttggcc aaccggacga ctgtcctgca tacaacttgt tgagcgaagg ccctcgatat
     5881 tttacaaagt ttgatttttt ccgggacgtg gaagttttga gaaactacca agggaatgcg
     5941 tatgtgcaga ttttccgaaa tatcaatgga gaagtagcag atatggcgtt agtaaaacct
     6001 ggtgcttgcc atccagtgat tgatatggat agcggggagc tttactacca agtaactgcg
     6061 actgacaaag gcagttacaa gcaagttatc tatgtacatt acatggaaat gcttcacttt
     6121 aaacaaccga ggtttggcgg cttggaaggt acagacccca caaaagtatt aacgaatacc
     6181 ctcggatatg atcgagaagt ccgaaaaatc tctttaagtc agcttaaagg aagtaatgaa
     6241 gggctaaaag ttaagtttgc tagcaatatg gatgaagaag ctaaaaaagc tacagttaaa
     6301 aacattgctg atttttatcg acaaaacggt ggactacttg tggaagaaaa cggtgtagaa
     6361 atcgaacgtt tacaacgaga gctggtagac agcaagcttt tagatactga taaagtatct
     6421 cgctccagaa tcgctatggt ctacaatgtg ccggagcatt tcatcgggaa taaccagtcg
     6481 agttactcct ctcaggaaca gctcaatatg gaatttttga cgtacaatct agtaccgacc
     6541 gttaatcaat atgaagcgga actaaataag aaaacactat cgagaaccga aaaagcaaaa
     6601 ggttatcgat ataagtttaa tgtcgcaagt ttgctaagag ctgacactca ggcaagggga
     6661 caattctatc aaatcatgcg acgaggcgga gcatactcag cgaatgatgt ccgaagattt
     6721 gaggacttgc aaccgataaa taaaaccggt atggatgatt accatatttc cggagaccta
     6781 tatccaatcg atatggatcc aacattaaga aaaacaacct cgtctaaaag cgtagccgaa
     6841 aacggttagg tttttttagt ttgcaccgaa gggaggtgga aaaatgaaaa aagtgacgtt
     6901 aagcggcgat gtcgtggata acgataccgc gtggctttat gactggtttg ggatcaattg
     6961 tatctcacca gggaaaattt ctgccgctct tacagaagca gcgggggatg aagtagaact
     7021 tgatatctca tcgaacggtg gggatgtcct agcggcaagc gaaatatata ccgctatccg
     7081 tgcctatccg ggaaaggtat ccggaaatgt tgtgagtatt gcagcaagcg ctgcaagtgt
     7141 aatcgcttgc gcttgcgaac cgcttagaat ctcacctacg gcacacatca tgattcataa
     7201 cgcatgggtg accactagtg gcaacgctga ggaattaaaa gccaatgcag aaatgttaag
     7261 cagtgtggat gagtctattg ttaatgctta cgagatcaaa acaggactcg atcggaaaaa
     7321 acttgctgat ttaatggcga aagatacttg gttaaatgct caaacagcag tagcagaagg
     7381 gtttgcagat gaaattatgt ttgcagaagc accagtaacg gtactcaatg cctctcaacc
     7441 ggttattcca aaaaacgcag taactaagtt gaaaaattta atactcaaag cggaagcacc
     7501 gcaagaagaa acactcttac agaaaaaact aaaagcctta aatggaggga aaaacgaatg
     7561 aatttagaac aattaaaaaa tgcgtgggtc gaggcgggaa gtaaagtctc tgacttaaat
     7621 gcacaactca atgcagcatt ggttgacgat gaaaaaacag aagaagatgt agtaagtttg
     7681 caagcacaag taaaagcagc acgggctaaa cgggacggat tgaaagagca agtggcaaat
     7741 atggaagccg aacaagtctt aaacgtcaaa aaagaaccat tagataaaaa agatgaaaac
     7801 ttgaaaaaca agtttatcaa agactttaaa gcgatggtca atggtgatcc tgctattatg
     7861 gctactttga catctaatac ggatgaatct ggtaatgcta tcggattgac tattcctgta
     7921 gatgtgcaaa cgactattca tactttggtt cgtcggtttg actctttgca agaatacgta
     7981 aacgttgaaa aagtgaccac taccagcggt tctcgcgttt atgaaaaatg gtctgatatt
     8041 acaccgttga ctgctttgga tactgaagac ggtgaaatcc cagcaaatga tgatcctgca
     8101 ctttacttga tcaaatactt gatcaaacgc tatgcaggta tttccacagt aactaacagc
     8161 ttgctaaaag atactgccga aaacattttg gcatggttgt ctaaatggat cgcgaaaaaa
     8221 gtagttgtta ctcgcaatac aaaaatcttg gcagctattg atggaatcaa agcggcacaa
     8281 aagaaagatg ttacagatgt tgatggaatt aaagatatcg taaacgtcca acttgatcca
     8341 gctatcgaag ctacatctat gtttattaca aaccaagatg gcttcaatgt tttagataaa
     8401 gtgaaacgtg ctgatggatc ttacttgtta caaaaagacg tagcttctgc aactggatat
     8461 accttcttag gtaaaccaat caagaaaatt gcttctcgtt tcttgccaaa caaaggtacg
     8521 caagctactc ctaaatatcc actgtacatt ggtgatctga aagaagccgt tacattgtat
     8581 gatcgcgaaa acatgagctt gctgacaacg aatattggtg gtggagcttt tgaaacagac
     8641 accactaaag tacgcgtcat cgatcgcttc gatgtgcaac tagttgatga tgaagcggtt
     8701 gttttggcta cttttacaac tattgcgaac gagacaccgg cgaaagttta aggagctgat
     8761 tccttatgat tcttgatcct aaaatggatt taggcgaaat caaaaacgca ctaaagattg
     8821 ataccgatga tgacgatgtg gaagtaagcc gtgcggcaca agctgcaatt gcatacatta
     8881 gaggggctat cgggaatgat aagccctctt tttataagca agaaaatgaa acggttgatc
     8941 tgattaattt agctatcctg caattagcag atcattatta caacgctcga tcagcaaccg
     9001 tgagtgggaa cttgcgagag tacgatttag gttttacaag cctaatcttg caactcaaag
     9061 caagttattt gctttttgtg gaggaggagt agcgtatgcc ccttatccaa acaggaaatt
     9121 taaatcaacg catcaagttt gtccgagata cgacggttaa ggatgaggac gggcaagttg
     9181 tcccgacttc tacaaccatt cttacttgct gggcaagtgt gcagacacaa cgcctgaacg
     9241 atattaagac gtcgattggt acggctttgg aaggaacact gacgttcatt atccgctacc
     9301 aacaaaaatc agagctgacc aatgatatga aagtgcgttg gaatggaaaa acgtttgaaa
     9361 tcattacgat tacgaaaggc gagtttgcga aggacttcac gacaatcatt gcaaaagagg
     9421 tttcaaaatg agtgtagaag tcgatgcaac cgaagtgtac aaagcgctta gggaagtaaa
     9481 agcaaacgtt caacgagtgg aaagcccagc acttagaaaa gctggggagt acgctcaaga
     9541 agagttacga caaaacacac cttactggga tggaacgaag tcaaacggta aacgtggttc
     9601 gtatatgcaa gaacatgcta agaaccatgt ggttacaagc tcgataaaaa acggattggt
     9661 agaagtcggc tatgacaaag atgtttcttg gcggatgcac tttatcgagt tcggaacaat
     9721 caaacaacgt ccaaaaggtt tcgtacaaaa aacacaaaag caaatcgaaa aacaagtaac
     9781 acaaatcatt gctgacgaag taaaaaggag gctaggactt tgaaaacggc agtatcacaa
     9841 gtctattcaa ttctgaatag caatgaaaaa acaaagaaca ttgattttta caccaatagt
     9901 gttccggaat cagctcaaac agtacctagc cttccagttg gcagaattac agagatatcc
     9961 ggcaactatg aagattttgc aagcaacaat cctttgacca ttcaatttaa cgtacaggta
    10021 gatgtatggg tgtcaaccat gaaagaggtt gatgcctttt attttgccct tgatgaggtt
    10081 atgaggggga atggttggca atgcgcatac acggaacaaa cagatgacga ggacttggaa
    10141 ggtgcaaagc ggattatcaa acgatatgta gcaaatattt cactaaatta aaaggagaga
    10201 aaatagatgg caacagtagg attcgagagc gtcatttttg gcgtaaaaac aggtgcaggc
    10261 ggcactctaa aagaattagt agcagataag tcgaaaggcg gagcgatcga agctaaaatt
    10321 actggattag gcgcaacttc taacacaaca tacgcttcaa acgtaccgtt cttcattgca
    10381 agtaaagggg tttcgtcgcc aaaagttacg cttgacgtgg cagacttaat ggataacggc
    10441 atttacagcg aaatcattgg tgctaaaacc gtggatggtg caaatgtaat tggttcagaa
    10501 actgaagcgc cttacgtgtc ggtagtcatg gttacagcga acaaagaagg aaaacgcttg
    10561 ttcatgggat tgacaaaagg aaaattcagt catccagata tcgacatgaa aacagctgaa
    10621 gacaaagggg tagaattgca aaccgattcc atcgaagggg aattcatttc tgatgaacgt
    10681 ggctatgtat acttaacagc cgtagaatca gaaggaatga ccttacaaaa attcaaggac
    10741 ttggtaaata acaaagaggg ggagtagtta accctgcatc tacaccaacg acagatacag
    10801 ggacaccaaa agaaccagaa ccaaaaattg atacacaagg ttagccattt ttggctagcc
    10861 ttattttttg taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg
    10921 aaagaaaaaa acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa
    10981 attttacgag aaagcggaaa aagcagacct aactgattta gaagcgattg attcaatgat
    11041 tgcaattaca gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg
    11101 tttgactgcg agcgagttag taccggcatt agaaagtgtt tttgaacaaa taaatgaact
    11161 gggaaacaat gaaaaaaagc agacggcgag caaaaagaaa taagtttttc tgaagctagg
    11221 aaagcaatgg atcaaatcta caaagattta atcgaagcag gttggacgat gagagatgtg
    11281 gacgaagccg actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa
    11341 tatgtagatg gtgctgattt catcaaacaa tttttatcgg ctgaagactt agtaaaactt
    11401 gaggaaggag gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga
    11461 ttagtttaga tagcactagt tttggtaaca cgctggacga aatcaatgcg aaagtcaaac
    11521 aagctgagtc gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg
    11581 aagcacttag tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag
    11641 taagagaatt aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac
    11701 aagttgctaa ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc
    11761 gccagttgaa cgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat
    11821 ctaatgaaat caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg
    11881 ctggtgatga atctggtgct tttgaagcaa aacaaaaagg gctagccaaa caaacggaat
    11941 tatccgagaa agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg
    12001 gcgattcagc aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa
    12061 gccaaatatc tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaag
    12121 aaatagaaga ttttggcgac aagtccacaa ggtcagctag gaaactggac ggactaaaag
    12181 acaaattaag ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata
    12241 acgctattag cagtgtagta agtggcgtgc aaggtttggt tggcgaagca gtaaacgcat
    12301 cggattcatt gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga
    12361 tagaaagctc gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag
    12421 aaattctgaa cacaaccgca caattggcat ctaatgggat tcctaactat acagaactaa
    12481 ccaaggcggc aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg
    12541 ttgccatgat gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc
    12601 aattagcaga tgcgataccg ggtgcttcag gattgttaca agacgctatg ttgaaaaacg
    12661 gagcttatac aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tccgacgagt
    12721 tcaaccaagc aattgtacag ttaggtatga atgacggagc agttaaggca gccacttcta
    12781 cagacacatt gagcggttct tgggagcaga tgaaatccac tgtaataaat gggctacaaa
    12841 gtattataga aaaaataggc gttgaaaata tcactggttt catcaacaga gtaacaaaag
    12901 ggattgaaaa ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa
    12961 cgtggatcgt tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta
    13021 cattagcagt aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat
    13081 tggcagcccc ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga
    13141 tagcttatac aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga
    13201 aaaacgtagt tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg
    13261 tgttgcagtg gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca
    13321 ttgaaatgaa gaacagcgtt gtgaatacgg ttcagaacct atggaatggc gtgaaaaact
    13381 tcttcagcaa tggcgttgga gatacgtgga ataaggtagt cggttgggta aaaaacattt
    13441 tcaacaaagc aactgaattg aagaacaacg tttctgatgt aatcggtaac ctgtggaatg
    13501 gtatcaaaga cacattccgt agaggtatcg atacggtatt caattggttt tcagaactac
    13561 caacgaagat gaagaatgcc attattggcg gtaaaaacgc cattgttgat gcgttcaaaa
    13621 gtattttcaa cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag
    13681 cttcatgggt actagaaaaa ttgggcgcag aacctctaca agaatgggat gtaccacaat
    13741 acgctacagg tacaccagca ggtgggcacc caatcaatgg tccaatgatg gttaatgatg
    13801 gacgtggagc agaaacagtt atcacaccag atggtagagc cttcattcct aaaggacgca
    13861 atgttgttct aaacgcacca aaaggaacgc atgtcttgac agcagaagag accgcgcaac
    13921 ttcaaggttc taaagctcct aagtatcgtt acaaaaaagg tactaacttc tttggtaaca
    13981 tgtgggatag tgtgaaaaag gttgctggta atgtaggtca cacaattaaa aacgtagtag
    14041 gtgacgtgtg ggactttatt tcagacccgg gagcgttagc tagaaaagta cttggggggt
    14101 tagatgtatt aggtggattg acaaagtatc cattagaagt aggtaaaggc atcctatcta
    14161 aagcaacaag tgcactgact gaaaaaatca ctgggttgtt ctcatctggt aacttagata
    14221 cctccgtagg aacaaatggc gtctataaat atttggctga tgttgctaag tctgtgatga
    14281 agaaattccc aggatttgtg gcaactagtg ggtatagacc aggtgaccct tattcacatg
    14341 gtaaacgtaa tgccattgat attgcattac caggcgtcac aggaggctca cctagataca
    14401 cagaagcagc aaactacgct tttgacaaat tcgcttccaa aattggttac gtaatcacta
    14461 atgggaaagt tcgtgaccgt tcaggacaat caggtcaacc agcaactggt gcatgggagc
    14521 catggcccga tggagatcac tatgatcatg tgcatttaaa cggtgtgaaa gacccacaaa
    14581 acactcaaat ttcaggagat agcgtgggag gcagtggggt agaaagatgg cgcaatgtag
    14641 caattagagc gttgaaaatg accggtcaat acagtactgc aaacttaaat gcattactaa
    14701 atcaaatgcg tacagagtca aatggtaatc ctaatgcagt taacaattgg gatattaacg
    14761 ccaaaaatgg aacaccatca aaagggttgc tccaagtgat tgacccaaca ttcagacagt
    14821 atgcaatgcc aggattcaac agcaatattt ttgacccact atctaacatc ttagcttcaa
    14881 tcagatacgc actatcaaga tatggctcac taacaaatgc ctatcgtgga gttggttacg
    14941 caaacggtgg aattgtaaac caacatcaaa ttgcggaaat cgcagaagga aacaagccag
    15001 aaattattat tccgttagat aaggctaaac gatcaagagc gatgcaatta ttgtctatcg
    15061 caatggacaa actaggagtt tctcctaaaa cttatggaaa tgcgacaact gtttccagtg
    15121 attacgaaat cctagaagcc attgaaaaac aggcggcaac aacgaatcag ctgttatcgt
    15181 tattgctggc atttttcaaa ggaaatagcc gaactgatag agatttagct ttagatattc
    15241 agaagatctt ggttaggagg atgtagatgc gaactgtatt actgagaaat agaataaacg
    15301 aagaaattga tttgtccaca gaagactatt ttgctactgg gttgagtaat atggggtttg
    15361 aagtaaaaaa agaacatgtg ggacagtggg ggaatttcag agaaagcagt gagagcgttg
    15421 aaatatctga atttcagtca tctgtaatca tttctgtgca tgggtttcga gaaaaagaac
    15481 tgtacaattc acttgtgcag ttcctatcgg aaggtccgtt tgaactggaa tttgcttttg
    15541 acggcgaaac aatggtaaga agatgcagtc taaaatcttt aagtaagact gaaatcgatc
    15601 cgaaaacatc gttactaaca gacactttag aactatattt cacatcaaac tggtattccg
    15661 taaaacgcga aaaattaatt cagcgaccga atgtagtgaa aacacgtggt aaggtttttc
    15721 cgtataaaag atcttatatc tacactcaaa acttgtggga gaaaaaaggt gtattcaaat
    15781 tcaataataa ttctgtatac ctaacgaata gcaaggaacg gatgtccccg ctaaaaattc
    15841 gtgtgattgg gaagtgttca aatccgtatt gggaagtaat ccaaaactca caaatcatcg
    15901 caacggatgg atacttcata gatatgactg aaacacagac tctagaagta tcaagccttt
    15961 ttgaagatac aacggcgatt ttaaaagata ttgcaggggt agaatcttct gtctaccaac
    16021 aacaggatta tacaaaaact aattttgttc aggccccaac tggagaattc agcattgtgt
    16081 ttcatgttgg gggggcagac gtagagattg agttatacga ggagcgtgat ctgttttgat
    16141 tttagcagtc acgctttttc atcgtgattt aatgctttat aacgaacatt tattctctaa
    16201 cagttttgaa tttggtgtgg acgagatcaa cgaagaggct agtagcttta cgatggataa
    16261 gtacgttcct gtaaaaacag gcgattttct actagcaaaa tatattccta gtggaaaatt
    16321 tgcatatttt ggtgtaatta cgtcgcagga agacgaaaaa atcagttgta aaagcttact
    16381 tagtttagct gatagtgaga taccgactgc acgtgtgtca ggagataact acgaagagca
    16441 tatccggcgg ttgattgaat actatttgct gaatgatcca acgaaacaac taaaagatat
    16501 tttagacgtc aaagcagaaa gtgcgacctc tcattcgtat caagctaccg atacgaataa
    16561 acacaagtta agtgcgtata ttctcaatgg tttcaaaaaa tacaacgtaa aatggtattt
    16621 caagggaatt caaaacagaa aaatttatac aggcatacgt gctgtaaatg aatcaattta
    16681 cattaaagac aattcttctg aatttagcga ttgggatgtg tttgttcagg cgccgggcgc
    16741 tggaaacgag aacaagctat taatcgttga taaagcaatg aaagatatag agaaaccgat
    16801 aatactgtca acatggtatt tggacgaaga aaacaatttg acacaagatg gatcgaaaga
    16861 aaatatcacg aaaccaactg tcaatttggt taatatctac gatcaaaccg cagaagataa
    16921 agcatcctac gaagatgtgg caaaatcaga gttgaaaggc aatacgtatt cgcatgaaat
    16981 caaagtgaac gttgtaagaa atgcaaaaaa tttgaatgtc gaaacgattg aaacagggat
    17041 gtttgccacg atttcttata aaggaaagat atacaagtcg gttttgacag cttggcgaat
    17101 atcaagtgat aaggagtttg tggaattgac tttcggaaac ataagaagtc gttttatgga
    17161 ttattttgaa gataatgggg gataaaaaat ggttagcaat gtggatggat atcaatttga
    17221 aaacgtgaaa gtaagcgcag aaaatgatgc tagactttat cacgttttat ataatcggaa
    17281 aaatcaggtt attgatggtt acgatcagtc tatgaattta tcttcaagcg gattaacagt
    17341 aaaagttgct gcaggagcag cgattattca aggtcgtatg gtcgttgttc gacaagaaga
    17401 aagtataaca gttccagcaa actcaagtgg ttatatagca ttaacagtgg atttgacaca
    17461 agaagttata cctggatcta ttcttccaga atcggaagaa tatgaatgga ctaataatca
    17521 agtcaagcta gaatttataa caaaagttat aaaaggtaat ttaaacaatg gtgataaggt
    17581 ctataattta ccactatgct cagttaattc tactggatca actgtttcaa tctcaaagat
    17641 atcggatagt tacgagctga ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt
    17701 aatgcatgat actcaaactg tccaaccttc aaaaaaaatt tggcagacag tttcaggttt
    17761 tttgttagta tggctcccat acgaaaacgg gcaagcaatt gaggatagat atgtaactac
    17821 gcctttttat aaggagcgtg taacttttac taatgtttta ggagaaattg tttcaggatt
    17881 tgatgactat cacaaaaagt ggtttagtaa acgaataaac tataattcga atactaatat
    17941 atttacaggt gcagcaagca atgcaagtgg agataacgca aatatggtgc ttaggtatat
    18001 agtttctttt tagttaggag gtatggaaga gtggcaaatt tagaaattaa attatctgca
    18061 aataagagac agccttatct acgtcaccgt gttgttggta gagttggtga tggggggctc
    18121 acaacaatca atgtacaact tcttgaggaa gatgaaatta caccttttgt aattaatcta
    18181 aacggtactt tgaaatttgt gggcgaagtt tcaaacggta actataccga gggagaacca
    18241 gaaataatcg attcgactaa tgggttaatt agttacacgt tcactaagtc aaatttcagc
    18301 acgagtcatc aattcaaaca agcatatttt gaatatgtag atcctaacgg caaaaaagta
    18361 acttttcaga acttcatcat agacgtgtta gaacgagtgg atattaattc ggagcaagcg
    18421 aaatactata tttcttcatt ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag
    18481 ttcattagtg ataaacaggt ccaatacgat caaatctact cgaaatataa cgaattagta
    18541 agattgataa atgaatcaga taagcaagta aatgatcgta ttgatcaaac caatcagcaa
    18601 atcggcgatc tcggcaagct gaaaaagatg tacagtaaca gcatcgactt tgggggctat
    18661 gattattcgg ggaatccgaa tttgttaagt aagctatcat acgacttaat tgaaaatcaa
    18721 aatacttcag ctggaacact ttctaagggt gaaaactcgt ttaaatataa taagatatca
    18781 gctgaagtgg aaggtggagt agagttatat tataaacgaa gaggtatagc taactggtta
    18841 ccctctaata aaacgcttgt aatgactgtt aagcttagag ctggagcgga ctatagtcca
    18901 gttgacggaa aacttatact gattagatat aggtatgttg acagtggaac tggcaagatt
    18961 gttttagact tacctattaa cagtaattcg ataactcagg aatggaaaga gtttagtatt
    19021 actggaacta ctccaacatt tagcccacaa gcataccatc cttggataca atttagggct
    19081 caagatgggg tacttgggga aatagaaatg agctatgaca tcaaaatcga agaaggctct
    19141 acagctacac catatcagcc taacttactt gatgactctt actggctagg taaaacaccg
    19201 ttgggtgaga atttgataac gaatgccaag ttcccaatta taacacgaag caatcctatt
    19261 tctggttttg atatttcaga agaattaata ataggtgaaa cttatacggt atctttaaaa
    19321 gggactaagc cagctactaa agaattccac ttatactacg gagaagcaaa ttaccagcag
    19381 ttgcagtatc aggcaacttt acttcctgtg gaaggattaa cagatgtgtg gagtgccact
    19441 tttacagcca gaaatactag tgaaacgaca aatttactta gagtagcgtt atggcaaaaa
    19501 cctaattctg caacatatga tactgttcaa attgactggc tcaagattga aaaaggcgac
    19561 acacgcacac caaatatcag tcagtttaaa tactttggtg aaggcttgaa agacagtaac
    19621 aatccgaacg actacagctg ggatgtcaca ccagagtata ctgaaaaaag cttgaataat
    19681 acggttagtc tgactgaacc gcaatccatc gaaggattga aaaatttcga agatggtatt
    19741 caatcaaaag gaaaatctgt attgacatca gacgacaaca aatatgaagt cgtaacctta
    19801 acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa aaccgtcact
    19861 atttattttt ttgcgttgaa tgggaaaagc agtggtggaa atgattcaac gatactaaca
    19921 attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt
    19981 tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga
    20041 aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta gaaaggcgtg
    20101 aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt
    20161 gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga ttccaaattg
    20221 gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggccgt
    20281 actggcggag agtatgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca
    20341 gatcagccac cgctcgaagg ggttcgttca gatggcgaac cgctaacgat tgctgatcag
    20401 atgtttgatc ctaaattgaa ccaatggatt attttagcga acgcgttaga tcacaacgat
    20461 ttaaacaatc tcaaagcgat gtacgaggct cttgaacatg aaaacggcaa cctaaaacag
    20521 ctaaatgcca aactcatgct aagcgatgta gcgattaaac aggaaaatac tgcattgaaa
    20581 gaaaaagctg acagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat
    20641 agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag
    20701 tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca
    20761 agttcgtcta ttcgttccac tatgtatcac agatgaagaa gcagatagaa ttattagcaa
    20821 agaagagagc gcatcttaag tgatgcgttt ttttgttgga aagttggtgg aacatgaaag
    20881 aagaagcgct ccaagacgtt gtggagagat tagtaagaat tgaaacaaaa ttagacaact
    20941 acgaatcact tagagaaaaa gctgatagtg caaaagattt ggcagacaaa gcctactcag
    21001 tagcgctaaa caacgcagaa gatatcaaag aaatgaaagc caataataaa tggtcgtggg
    21061 gttacatgat cggtttaggc attacgatca ttggctattt cttgactaaa ttgtaaagga
    21121 gttaagaaga aatgatttta cccgataagt attatcaagt cattaaatgg acggttttaa
    21181 cagtattacc agctgcatct gttttagtag ccacgttagg aaaagcgtat ggatggaatg
    21241 gaacagatat gacagtactc actatcaatg cagtagcaac atttttaggc gttatcactg
    21301 gtgtgtctgc atataactta aaaaaatagg aggaaaaaaa tgaagaagaa aatcatttta
    21361 tcattgagcc taataatggc tcttttttta ttgccaatta acgggttcgc ctatacgatc
    21421 aacaatgaat ttaatttagg tgtaaatgaa ggtagctcac aagtagcaaa taaccaatac
    21481 atcctattgc atgaaacagc aaacgaaact gcgacaggac gaaatgaagc gcagtacatg
    21541 aaacgttctt ggtacaatgc ctatacagcg tatattgttg gcgatggtgg gatcgtttac
    21601 caagttggac aacctggtta tgtacaatat ggcgctggtt catatgctaa tgcaaatagt
    21661 cctgtgcaaa tcgaactgca acatacgcac gataaagtaa cgtttgaaaa gaactacaag
    21721 gcatatgttg aattagcgag agattccgct aataaatacg gtattccact tacattagac
    21781 acaccgtata atcaaccagg aatcaaatca catttatggg taactcaaaa catttggggc
    21841 gatcacactg atccgtatgg atacttatca gaaatgggtg taagtaagga aaaactggct
    21901 tacgatttag ctcatggatt tacagacgaa aacccaacga cttctgagaa caagcctgtc
    21961 attgatccaa cccgagcagg tgcagcaaat ccaactttat cagatggaac gaaccattct
    22021 cacattgatc agtttggaga aatcgaaaat gcgaacttac acgtcgctgg atggcacatc
    22081 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
    22141 cgagtaagag ctgatggaat ttatagaccg gatgtaaatc aagcttataa tacttctgga
    22201 aacgttggct atcatgtatc ttttaacatg cgcaatttcc ctaataagaa agtatacgtc
    22261 atgatgcggg caacgaatga tccagaagga aacactaaag gcggtgcgca agatttccat
    22321 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag
    22381 tacatagaat tgaaaattaa cgttaaatca aaaaaatatt tacaagatgt ttatttaagt
    22441 agataataaa agaaaaaagc aggtgaaatt gtgacagcag aaattggtat tatgaataaa
    22501 agtggaatcg tgttagcttc agattcagca tctacgattg gagatagcaa agtatataat
    22561 actgcaaaaa aattatttac tttggattcc atgcattctg taggtatcat gatatatggt
    22621 aatgctgaat ttaatggtat tccttgggaa ataatcatta ctcaatataa gaaaagcatt
    22681 ggtagttctg tttttaatac tttggaagaa tacgcggata attttataga atttgtcaaa
    22741 acagcatctt ttattagaag tgaacagacc gagcaagaac aaatgattgg ggtttttcaa
    22801 aaaataattt caggtttatt tgagagtata gaagtagata ttaacttttt aataagccaa
    22861 ggtacacaaa tagacaaaga tgtcctagtg aagttattac aaacaaaaat gaataccaa
//
LOCUS       DAZKNM010000028        22553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000028 DAZKNM010000000
VERSION     DAZKNM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.028"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(262..2154)
                     /locus_tag="ACG8M9_000895"
     CDS             complement(262..2154)
                     /locus_tag="ACG8M9_000895"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377685.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2151..3878)
                     /locus_tag="ACG8M9_000896"
     CDS             complement(2151..3878)
                     /locus_tag="ACG8M9_000896"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377686.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3894..4544)
                     /locus_tag="ACG8M9_000897"
     CDS             complement(3894..4544)
                     /locus_tag="ACG8M9_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0377687.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4748..5044
                     /locus_tag="ACG8M9_000898"
     CDS             4748..5044
                     /locus_tag="ACG8M9_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0377688.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5216..5836
                     /locus_tag="ACG8M9_000899"
     CDS             5216..5836
                     /locus_tag="ACG8M9_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0377689.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5928..7094)
                     /locus_tag="ACG8M9_000900"
     CDS             complement(5928..7094)
                     /locus_tag="ACG8M9_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0377690.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7106..7459)
                     /locus_tag="ACG8M9_000901"
     CDS             complement(7106..7459)
                     /locus_tag="ACG8M9_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0377691.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7587..8609
                     /locus_tag="ACG8M9_000902"
     CDS             7587..8609
                     /locus_tag="ACG8M9_000902"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0377692.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8628..8789
                     /locus_tag="ACG8M9_000903"
     CDS             8628..8789
                     /locus_tag="ACG8M9_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377693.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9048..9482)
                     /locus_tag="ACG8M9_000904"
     CDS             complement(9048..9482)
                     /locus_tag="ACG8M9_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377694.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9592..11451)
                     /locus_tag="ACG8M9_000905"
     CDS             complement(9592..11451)
                     /locus_tag="ACG8M9_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0377695.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11452..11592
                     /locus_tag="ACG8M9_000906"
     CDS             11452..11592
                     /locus_tag="ACG8M9_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0377696.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11569..12729)
                     /locus_tag="ACG8M9_000907"
     CDS             complement(11569..12729)
                     /locus_tag="ACG8M9_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0377697.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13036..14460)
                     /gene="araA"
                     /locus_tag="ACG8M9_000908"
     CDS             complement(13036..14460)
                     /gene="araA"
                     /locus_tag="ACG8M9_000908"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0377698.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14476..15174)
                     /locus_tag="ACG8M9_000909"
     CDS             complement(14476..15174)
                     /locus_tag="ACG8M9_000909"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0377699.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15131..16792)
                     /locus_tag="ACG8M9_000910"
     CDS             complement(15131..16792)
                     /locus_tag="ACG8M9_000910"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0377700.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17010..18410
                     /locus_tag="ACG8M9_000911"
     CDS             17010..18410
                     /locus_tag="ACG8M9_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0377701.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18662..19741)
                     /locus_tag="ACG8M9_000912"
     CDS             complement(18662..19741)
                     /locus_tag="ACG8M9_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0377702.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19827..20786)
                     /locus_tag="ACG8M9_000913"
     CDS             complement(19827..20786)
                     /locus_tag="ACG8M9_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0377703.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20798..22309)
                     /locus_tag="ACG8M9_000914"
     CDS             complement(20798..22309)
                     /locus_tag="ACG8M9_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0377704.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22331..>22553)
                     /locus_tag="ACG8M9_000915"
     CDS             complement(22331..>22553)
                     /locus_tag="ACG8M9_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377705.1"
                     /translation="WIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLL
                     IFLFPPFIFIFAGGVWKLVSIILTIGNER"
BASE COUNT         6421 a         4778 c         3987 g         7367 t
ORIGIN      
        1 aaaatagctc ctcctatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga
       61 acaccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagatacaaa
      121 acggaaaaat agcttctcat attttgccgt tttttgagct aataccgaag gacagctgat
      181 tgaacaccgt ttattcggaa aaagggactg tgacacgact tttgtcacag cccctaaaaa
      241 aaataataaa tttgtactat tctatgaagc ttcttcttca gaaaactgac tgttataaag
      301 atctgcatag aatccgtctt tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga
      361 accatgattc atcacaataa tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac
      421 aacgaaactt gtccggtttt ccaataggcg gttcatcgct ttttgaatca gtatttctgt
      481 tcgtgtatct acactagatg ttgcttcatc aaggatcagg acatctggat ttgctaagaa
      541 tgcccgcgca attgtgatta gctgacgctg cccttgcgaa atattgcttg catcctcgtt
      601 taatacagtg ttatatcctt ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt
      661 agcagcctca atgatttgtt cgtctgttgc atcgtcgttc ccatatttga tattgtcata
      721 gatacttcct gtaaacagcc aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc
      781 atcacgtgaa agatcacggg tatctacacc atcgtaacga atactacctc cagaaacatc
      841 gtagaaccgt tcaagaaggt tgatcagtgt cgtcttccct gcaccagtcg gacctacaat
      901 agcgaccatc tcaccaggtt tgacatccaa gttaaaatca gtcattagca agttatctgg
      961 tgtgtagccg aattgaacat tttcaaaacg aactttgtat ggtgtatctt tttcttctgg
     1021 aaggccagat ttttcattgg acatctcttc ttcatccaac acttcaaata cccgttcggc
     1081 tgaagcaact gttgattgga tagtgttcat caaacttgca agttgagtga ttggttgaga
     1141 gaactgtgtt gtatattgca agaacgcttg aacatcccct aatgtcatgt tcccatttgc
     1201 aacttttact ccgcctagta cagcgacgaa cacatagcct agatttttaa cgaaattcat
     1261 caacggcatg atgattgccg aaataaattg agctttccaa ccagcggcat acagattctg
     1321 attttctttt tcaaatactt cctggtcttt ttcagtatga ttgaatgttt taacaatggt
     1381 atgaccacca tatgtttctt ctacttggtt attcaataaa cctaaacttt tttgctgatc
     1441 tgcgaaaaac acttgtgatt tcggcgctac gatcatcacg atgatcaagc ttaaagggat
     1501 catcaataac gcgactaacg tcaattgcca gctgattgaa agcatcatca cgatgacccc
     1561 aatcaatgtg actgcactag ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat
     1621 gttatccata tcattgattg ctcgagacat gatatcccca tttgtatgtg tatcataata
     1681 ggaaatcggt acacgattca ttttggcttc caaatcatga cgtaattcat aaactgttcg
     1741 ttgggaaaca cgtgtcataa taaattgctg taaaaagttg aatacagcag aaatcaaata
     1801 catcacgatt actgtcgcaa tgatctgcgc gattttatca aagtctatcg gaaattttgt
     1861 tacttgttgg ccttgttgca tctgttgagc gccagccatt acacctttga atatttctgt
     1921 ggttgctttc cccaagacct ttggtgtttg gatttggaag atcacggctg ctattgctag
     1981 tacaaataca gcgatgatcg caaccatacg tttagacata tagccaaaca atcggcgtac
     2041 cgttccccag aaatttttag gcttttcacc ttttacaccg agatttctgc caggtccacc
     2101 atgtcctgga ccgcctgctg gtccatgaga gggacgtgtg tttgttttac tcatgctaag
     2161 tcctcctctc ttagttgtga atgaacgatt tcttgataag tttcgtttgt tgctaatagt
     2221 tcttcatgtg ttcctttgcc aaccagtttt ccttcatcta gcacaaggat catatctgca
     2281 tccattactg tacttactcg ttgagcaact aaaacggtga tactttcttt catattcgtt
     2341 ttcaatgctt ggcgaagatt ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca
     2401 aacacataga tatctgctgt tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt
     2461 ccaccagaga agtttcctcc tccttgttcc acatggctat ctaagccatc ttccatctct
     2521 gaaacaaaat ctttagcttg tgcgatttct aatgctttcc aaatcatttc atctgttgca
     2581 tccggcttac catactgcat attgtctcgg atcgttccag agaacagaac tgctttttga
     2641 ggaacaaagc ccattaattc tcgcaaatta tattgagtca tgtcactaat gtccgttcca
     2701 tttatttgga tcgaaccaga ttcgatgtca tataatcgag ggatcaaatt gactaaggtc
     2761 gttttacctg aaccagtccc ccctatgatt gcgacgatct cgcccgactt ggcttggaaa
     2821 tcaatatctt ccaatgcaag tttttctgct ccatgatatc gataattgac atgattgaag
     2881 gccaatgttg cgttttttcc agcaaaagaa actgttttag gattttcagg gtcatggata
     2941 ccgatttttt catccaacac ttcattaata cggtcagctg aagcttgtgc acgaggcacc
     3001 atgataaaaa tagcggaaag catcataaag ctgatcaaga tctgcatcgc ataagtcata
     3061 aatgcaatca gattaccgac ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa
     3121 gtgattgcta cgtttgtacc gctcatgatc aatgtcatca ttggcatcat caatgcgacg
     3181 atcgtattaa ctttgattgc cgtatttgta taatctttat tcgctaaatc aaaacgattt
     3241 tcttcaaagc gtgtcttatc gaatgctcga atcactctga cccctgtcaa tccttctcga
     3301 aataccaggt ttaaacggtc agttttcttt tgcatacttt tgaataatgg aacagcaaag
     3361 tacatgattc cgccgatcaa aataatcatt actgggatga caactaagaa gatttttgtc
     3421 aactgatgat ctttttgata agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg
     3481 atcatcatac gtaaaaacat ttgtgtaacc atttggattt gagtcacatc attggtcgtt
     3541 cttgtgatca aagaggccgt tccatattta tcaaacgcat cttttgtcag actctctgat
     3601 tttttataga catccgtacg caattgctta cccagtttct gtgattcacg agtagcaaaa
     3661 aatgtatttg cgatagcagc taagatactg atcaaagaaa agccgatcat cacaaaacct
     3721 gtatgccaaa tataatcgac atctccttgc gctacccctt tatcaataat attggaggta
     3781 agtgtcggca aatataaatc tgccagtact tggatcacca tgaaaactgc agctgcaatt
     3841 gctgaagtca aggagatccg ttttacgatt tttagcatct agttcccttc tttctattta
     3901 tatactccgt ttttcagcca agaaagtttc aaattgaatt cactgattgc ttgctctact
     3961 gagtaatctt cagatgttga aaataagcga tacgaatgcg caattgttgt aaatacagta
     4021 ttcatcacta attggatcag taattccaat tcatgatcat tttttacttt cagcttcgtc
     4081 cgatcgatca attcaagcaa ctcttttgca tcttgtttgt gttctttatg tggatcgtga
     4141 ggcgtttttt ggcgactcgt atgaggagcg acttttcttg aggcacggta atccatgttc
     4201 ataaacaagt tacgataaaa agaagcattc tcgccttcca aaatctcata aatcatttta
     4261 gaaaaatagg attcgaatcc tttgaataga tcaccatcgg attctttcat tagttgtaac
     4321 atatctttag agctgtcacg tctcattgtc tcaaagtaat aaaaataaag atcctcttta
     4381 tcttcaaaat actgatagaa actgcctcta gggatatccg ctaatttgac gatattagca
     4441 atcgaagctt cttttaaggg tactctagaa aactcgattc tcgcagcctc gattagtctt
     4501 ttttgttttt ctttaggcaa atggaaaaat gtctgtgtcg gcatatggat cctcctttct
     4561 ttcaagatga cacattgtca tatgacgctg tgtcattata acacctccta cctgttttgc
     4621 aagacatttg catagttttt ttcattatca tccgatacat taaaaaagtg ataagaggaa
     4681 tccttccatt tgaattaatg agagttgccc attaaaatag ccacatacta aacaaaggat
     4741 gagataaatg gcccaatcaa ttagtgtcca agacttcaat aaattgcctc tcgatgctga
     4801 cagcatcgtt ttagatacaa gaaatacctt agattacaca aaaaaccact taagccactc
     4861 gctttctatc cccgctgcta tcttgccaag acaattaaaa aatctagatc caaagaaact
     4921 ttattatatt ctaagctata caggcaggcg atcagaagca ttagccgatc aacttactgc
     4981 taatggtttt cgtgctgttt atatcattgg tgggatggaa gctcttcagt cgagtgttgc
     5041 ttgaatattg cttgttagat tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt
     5101 ttataccctg tcttttttta gttatttatt cttcaacctt atcaatatga cttgatagcc
     5161 ttttcttttt ctcatagtta taacaatcat agcaataaaa ggagaaggta agcatatggc
     5221 atctgtttta gtagtcaaag gtcatcccct aactgcagaa gaatcgcgca cagttaaggc
     5281 attgacgtct tttttaacaa gttataaaga aaatcatcca gatgatgaag tgactgtttt
     5341 ggaactttat cgcgatgata ttccggaaat cgatgaagaa ttattatcag gttgggaagc
     5401 attacgtgct ggtgcagaat tcacttcctt atcagaaagt caacaacaaa aaattgctcg
     5461 tttcaatgaa ttgactgatc agttccttgc tgctgataaa atcgttatcg caaacgcctt
     5521 atggaatcta aatatcccaa caaaattaaa agcttggttc gataccgtca atgtagctgg
     5581 aaaaacgttc cgctatacag aaaacgggcc agaaggatta gtgacaggta aaaaagcact
     5641 gcacatccaa tctaatggcg gtgtatacaa cggacaagac tttgcctctc aatatgtaaa
     5701 aggtatttta aactttgtag gcatcgatca agtagatcag ctattcatcg aaggaatcga
     5761 ttatgaccct gaccgtgcag atgagttgat gcaaaatgca ttagataaag ctgctgcttt
     5821 aggtaaatca ttttaaaaat tgagataaat gcatacaaaa gagacagagg aaaaaatctt
     5881 caaagcagca tttgtacctt ggatattttc ccatctgtct ctttttgtta ttctttctgc
     5941 tttttcaacg gattttcttc ataaagagaa gagattgttg tgtaccattc aaaaacatga
     6001 gtaatcacta ctttagctgc tgcataaaca ggaatcccca agatcacacc gatgattccg
     6061 aacaatttcc cagaagtcag cagaactaat aaaatcgtta ctggatggat tgctaactga
     6121 ttccctagta ctaatggaga gatcaagcgt ccttctattg tctgctctac tgcaaaaacg
     6181 atcagtactt tgatgatcat gaatggacct gctacaatgg ctaaaaagat agccggaatc
     6241 atcgctaaaa atgatcctaa gtaagggatc aggttcaaaa aacccgcaat aatccccaac
     6301 gtgacagcat actctaaacc aatgaccgca aaaccaatca taaacatgat tgccacagca
     6361 aaagccacag ttagttgtcc tcttatataa gaagaaactt gactattcat ttcactcagc
     6421 acattcaacg tcggtttttg catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat
     6481 tgtttaccgt ctttcaatag ataaaataag ataaacggca tcgtcagcag agccactaaa
     6541 atagtcgcta ccgcgccgac aaaacttcca agactctgaa ctgtactctt agatatatct
     6601 tggatcatat cgcctgccga attcatgaac ttatcacctg ctgtttccaa ctgttcgcga
     6661 aattggttga acagcgggtc ccgcaaaatc tcggttaatt tattatcaat cgtatctaca
     6721 tattgaggga agttactgat gaagctcact gtttgttcct gaatcttagg gatgatcact
     6781 acgctccccc atgcgattag tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc
     6841 cttggtactt ttcgcttttc aaagtaatca actaccggat tcatcaagta atacaagatt
     6901 cctgctaaaa tgataggcag acctacgatt gctaaaaatt gccagattgg tgcaaacaaa
     6961 tatgaaactt ttgtaaataa aaagacgatc agcaatatta acaatacaac aagtaaggct
     7021 gtgactactt gattatttaa aaaccagcgc caaaaccacg ataccggtct gttcttctta
     7081 ttttttcctt ccataattcc tcctgttagt tataaaaaat ttggcttccc aaaccaatag
     7141 caggcagtct atgcggatga agatagattc catttgcaat ttgtccttct tctggacaag
     7201 catcaaaaat caatgatacg tgagctactg tctctaaatt cccattggcc atcggaccga
     7261 cataatcgat cgtatatacc tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa
     7321 aaggttcgtc ttttgatact tcttgaatca ccgaatattt tcgtaaggca gctgttgctg
     7381 ttttatcgaa caaaatcagc atgggttctt ccttgtttat tgcttctgtc ccaatttctg
     7441 taatctttgc ctgaatcatt gcactctctc ctttaattgc tttcaaaaac agtataacac
     7501 attctcattc tttagccgtg tggaataatt atgtgataga ataaaaacga aaactatttc
     7561 attacaatta ggaggaatca gctcatatga tcgaaaagat aattttagga acctatacgc
     7621 gtcgcgaaag taaaggaatc tatacgatcg accttgatac agaaaaagaa gaattagcca
     7681 acctttcatt agctgttgaa gaaaatagcc ctacctatgt agcaaaaagt aaagctggca
     7741 atctttacac agttactagt gtggatggat taggtggtgc cggtgcgtac gataaggatt
     7801 ttcatttttt gaatgcggta acagagtctg gcgctccttt gtgttatgta gcggttgatg
     7861 aagcacgtca attactatac ggggcgaact atcataaagg tgaagtaaat gtctaccata
     7921 tattaggaga tggcgaactg aaagcagcag attcaatatt ccatcaagaa gcgacaggtc
     7981 ctcataaaaa ccaagatcat gcgcatgtcc actatacgga tctgactcct gaccaacgat
     8041 tagtcgtatg tgatcttggt acagatcgtg tgtatactta tgatgtgtcc gaaaacggca
     8101 aattgaatct tgtaactacc ttcactgcag agccaggaac aggccctcgc catcttgtct
     8161 ttcatccaaa tggacaattc gcttatttat ttggtgaatt agacagcaca gtccgcgtgc
     8221 tttcttataa taaagaaaca ggtgcttttg aagaattaca gaaaatatcg accttgccag
     8281 aagaattcga cggtgaaaat ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc
     8341 tttatgcttc taaccgagga cataactcga ttgctgtatt cgcagttttt gaagatggtt
     8401 cccacattca aaacaagcaa atcatctcaa cagaagggga ttttccccgc gatttcgcac
     8461 tagatccaag caataatttt gtcgtgtgtg ccaatcagaa caccgataat ctgacattat
     8521 atcgccgtca tcctgaaaca ggattactgg aattactgaa aaaagatgtc tatgtaccag
     8581 aatgtgtctg tgtttatttt gatcgttagt aagaaaagga gaatgaaatg aatcagcaag
     8641 acgtattaga cagactacgg gaagaactaa atattccttt ctttgatggg aaaatcgaag
     8701 ataaagagta tagcgaagag gaatatcaga aactcaaagc cgatttacag aactactttg
     8761 aacaatatgt acagaatgta gaaaactaat cagctttcca tactatctga aaagaacatc
     8821 aggacaagac ttttccgtct atcctgatgt tcttttctac aaataaaaaa ccgccgagat
     8881 ggttagactc gacggaaaag gagtatttac tcgattttga gtaagtacaa ggttgttgtt
     8941 ggttataggt atgtcattat cataaaggat tttgatcact ctatcataaa ggtaatatta
     9001 catttatatt agataactct agtttttatt gttatcaaag atttctttta ttttttcaac
     9061 aacaaattag tcattttttg gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt
     9121 gcttcagatg tattgaatag atcttcatct acccctgatt caagatcgat actttggatc
     9181 atttcttcac agcctgctac atatgaaaga taagcttttt caaaattttt atgaatgccc
     9241 attgcttttg ctggtggacg caaagtactg actttcttaa gcattgcccg atattttttt
     9301 gttccttctt caaataacag gatgatctct tcaagagtcg atttgtccag ttcacttgtt
     9361 tttccttcat caattgcgtt tctgatcact tcatatttcg gatgcattcc ttctccgatt
     9421 gcttctgttt ccttgacgat ttgatcgatg gactgcacat aatagccaat attcggtaac
     9481 atgttcgttt tcctactttc tccattgttg ttattattta ttctacttgt ttttatcctc
     9541 aaatgacaag tacttgaaaa aatttaatgg tttctataca gctcgccatt cttattcgtg
     9601 ctcaaagaaa ccatctgcta aactatattg tttccctgcg acaatgtcat attggatcaa
     9661 ttgatattcg tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt tataccactg
     9721 tccgttttga tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta atgataaaag
     9781 cagctgataa aagccatttg ttgtctgatt cgtctgttcc attagattcg ctgcaaaata
     9841 aaacggactg gtgattgcat cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa
     9901 cataagaaat tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga tcgtatctga
     9961 atagatccct ggaagatgat ccccccaaaa aacaactaaa gtccttctag aaagattctt
    10021 caattcttcc gtgaactgct tcagtgccgt gctgctatag gaaatatctt gcagataatt
    10081 ctctaagtca agtgtcccac ttccatctga agtttttgca ggatagtcta gattatcata
    10141 ctttccatcg tagggcatat gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt
    10201 ttcttccttg agaagtgtca ttacttcttt gtaagctgac tcatcagaaa tatatggatt
    10261 attctctatc gtatccgtat aggtcatcgt acgctcgctg atgaactggt caaatcctaa
    10321 tgtctgataa acatcctctc ttttatacat cgaagtattg tatggatgga tcgctgtcgt
    10381 ctggtatgat tgtgcgttta acgctgaaac gatagaagga agctgatcca tttttggtac
    10441 aagcatcgtg taaggggtgg tgagttgcgc attaaataac gccatagaaa aaccagtcaa
    10501 tgcttcaaat tcgatatttg ctgttccacc gccatagttt tgagaaagca tattgccact
    10561 atatgtttga tcagctactt cataataatc tgccaatggc tcaccagata cttctacgcc
    10621 gtttaatcga ctaggatctg agaaactttc actcatcacg aacacgatgt tcggcgactc
    10681 ttcctctaca gttttatttt tttcatcggc tagcttctgg tatttttcag tgatttcttt
    10741 gatcttttct ttcgaatatc cttcaggttc atccattggt tctaccttca gattgtacaa
    10801 aaacccaccg ataaaaccag tgttatagta gttcattttc tggctgtaag gtatccatag
    10861 tgccgtttta ttatatgctt ttcgcaacaa attatcggga ttgttgaaat tactgatata
    10921 ccctagcagt ccaattgcca caagcagtgt cgttaatcgg atcaattgga atcgccttcc
    10981 ttttttcagg cttcgaaaca tgtaccagca aaacaatcct atcacggttc ctgcggcagc
    11041 taagatcact gtaaacagca tagtgccggt catatctttc agcaatccga tttcagtgat
    11101 catcttcaaa tcgtctggat aaatcggttc ttgccgataa ctcattttca tataatttgc
    11161 aaaacctaat acaccgatgg ctacgacata cgtaagggaa cctaaaggga cagatcctgc
    11221 tagtgcaatc agaaaaataa gaataatcag caagacgaga caggctaaga agaatttttc
    11281 cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg gatagatcat tttgacacca
    11341 ctgcaaaaat aaattactaa aaacaacaag aaaaaagata ctgatgattg ctgctgttcc
    11401 ttttaaaatc tttttctgtt tggtcgaaag tctgcttata tatcttttca aatgcttgct
    11461 ccttcatttc tatcaataaa agaatgttgc ttctcttatt ttagttgtac gaaattcgaa
    11521 agtaaagaac ggaaagaaga attaatgaaa tccaaatatt ctctttcttt atggcctttc
    11581 tggtttcttt aataagcgga cctttcgtac tccagaaatt ttttctagct gttgtttcac
    11641 ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga catgctcatt
    11701 cttatggtta cgcgatatat ctgcgatcgc tagctcgttc tcattcagca gagaaaatat
    11761 ctctgctaat atattggttt tcttttgata aaatatagtg aatcgaaacg gcgcttggaa
    11821 aaacattcgt gcggatggat aattgaccgc ttctctaacc gtaccgaaca aaagataatc
    11881 ttttaaggct tcaactgcca aacgtccgct gtctgccaaa gcttcctgag tcgatccccc
    11941 tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg gaaaatcagt
    12001 caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt tatcaacaat
    12061 ttcacttctt cctacattga taagtacagc atcttttttc attttttcta tattcgcctg
    12121 atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt aatccgaggt
    12181 agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat cgcggataga
    12241 ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg tgcctatcgc
    12301 tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat aagcacttct
    12361 tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca tacgacttgc
    12421 ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt ttactgcatt
    12481 agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt
    12541 gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg taagccattc
    12601 atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat ctattatttt
    12661 gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa tcaaatgaac
    12721 gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca attagacaac
    12781 tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta ggctgagtga
    12841 taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc aaaatattga
    12901 gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa gactaaattt
    12961 caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac taagtggctt
    13021 tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt ttgcccacgt
    13081 ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc cacctgcctg
    13141 aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca cacgtgcaac
    13201 aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga tcagcagttt
    13261 ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt tcccatcaaa
    13321 tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa cgattcttgg
    13381 tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga ttgcttcttg
    13441 gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc ctcgagccat
    13501 gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag caaaaccgta
    13561 tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc catacaggtc
    13621 ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc gaataccgat
    13681 ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat aatcaccata
    13741 gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt cgtctgatac
    13801 gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc aaccaaattg
    13861 aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt tgtcaccaaa
    13921 acgagccact tttattccaa aactttcatt ataggctact gcaacatcca tccaatcagc
    13981 aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat tttgtttatt
    14041 taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat ttaaattcat
    14101 gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag ctaaatgaag
    14161 caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg agaaagtatg
    14221 catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca tgatttttgt
    14281 gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca attggccatt
    14341 ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca gtgcttcttc
    14401 cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa tattcaacat
    14461 aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa
    14521 ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga gctgtctggt
    14581 aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg cactttgcgc
    14641 atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg
    14701 atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat attcgtaccc
    14761 gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat aaaaagtatc
    14821 tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga tcgtcgccca
    14881 tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata aaacagcatg
    14941 agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt ccaagtccac
    15001 aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt tgataacgaa
    15061 atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga cgagtccgtg
    15121 tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca gcattttcta
    15181 tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt
    15241 aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg atggtgattc
    15301 cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc ccatgtcgat
    15361 ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg gctgtgtcca
    15421 tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact tctggtgttt
    15481 taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat ttcaatatat
    15541 ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg aaattcgcta
    15601 aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt actcctgtaa
    15661 tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta tcgccttcta
    15721 gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata cccaaagaat
    15781 tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag ttattcgtat
    15841 gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc ggatggacat
    15901 tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc gaaatgttcc
    15961 ctgtccgttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc ccttctggcg
    16021 gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa gcccctgatt
    16081 cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg tgatccaagt
    16141 caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt tcttgatggt
    16201 aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg cctagtactt
    16261 tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt
    16321 tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc catctctgcg
    16381 gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg attgtatttc
    16441 gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga taaccgtgca
    16501 tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag atttcgaata
    16561 cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct tctaatgaat
    16621 aggtccagat accattttct agtttgtttt cccactcata actaccagcg gcaataggag
    16681 taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca atccctaaag
    16741 atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc ataatgattt
    16801 aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct tacaatgatt
    16861 attgtatact tttgtacgta catgtcaagc ggttatttac aagaatacaa taaaaaacac
    16921 ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga tcaaccgcct
    16981 tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt
    17041 ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt ggtgttatga
    17101 ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct tctttgatag
    17161 gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta tctggtcttc
    17221 tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt tttatggctg
    17281 gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt gcagcaagga
    17341 ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa
    17401 tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg attgcttctg
    17461 gcatgctcct ttcctatgtt gcagattatt tgctaaaagt gttgcctgaa acgatggctt
    17521 ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc ggtgttttag
    17581 ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag gcaaaaagag
    17641 tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa atccagctga
    17701 atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta gaaaaatatc
    17761 gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa ggagctaatg
    17821 ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg
    17881 cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg ctctttttgg
    17941 ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc gtgatgggac
    18001 tttcattcat cctaccagct gttttaggaa ctgtactgga tgctcataca aattcgttgt
    18061 tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg gctccactga
    18121 catgggtaat cataggcgaa atattcccac tagctgtccg cgggcgagca tctggcctag
    18181 cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct gtgatgactg
    18241 cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt ttaggggttt
    18301 tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa
    18361 gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa agaggttgtg
    18421 acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt cacaaggaac
    18481 aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc agttggtagg
    18541 agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag
    18601 ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa aaacccgtct
    18661 cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca tagagaatat
    18721 ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca
    18781 acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg cttaaagaag
    18841 aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt tctgtcaact
    18901 tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct aaagcttgtt
    18961 cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag
    19021 taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg ataaaacctt
    19081 tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga cgctgatgtc
    19141 ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct aaacaaatat
    19201 aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc
    19261 tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga
    19321 tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat
    19381 cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc
    19441 ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt
    19501 ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc
    19561 ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat
    19621 gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt
    19681 cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca
    19741 tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt
    19801 caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct
    19861 ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct
    19921 aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat atcaaagcac
    19981 ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa
    20041 gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc
    20101 tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta
    20161 atgaatattt tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt
    20221 ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga
    20281 ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc
    20341 cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc
    20401 cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga
    20461 ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt
    20521 gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat
    20581 tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat
    20641 tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta
    20701 aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata
    20761 ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta
    20821 cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat
    20881 agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc
    20941 tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta
    21001 tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta
    21061 cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa
    21121 tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc atcacataat
    21181 aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta
    21241 ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta
    21301 atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga
    21361 cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta
    21421 gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt
    21481 cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct
    21541 catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag
    21601 ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg
    21661 atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact
    21721 tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca
    21781 cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt
    21841 tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt
    21901 ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt
    21961 tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa tttggttcta
    22021 tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca
    22081 cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt
    22141 cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg
    22201 gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc
    22261 gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca
    22321 acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc
    22381 caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata
    22441 acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt
    22501 caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc cag
//
LOCUS       DAZKNM010000029        22336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000029 DAZKNM010000000
VERSION     DAZKNM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.029"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG8M9_000916"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG8M9_000916"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0377706.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG8M9_000917"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG8M9_000917"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0377707.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG8M9_000918"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG8M9_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0377708.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG8M9_000919"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG8M9_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0377709.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG8M9_000920"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG8M9_000920"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0377710.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG8M9_000921"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG8M9_000921"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0377711.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACG8M9_000922"
     CDS             complement(8557..9027)
                     /locus_tag="ACG8M9_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0377712.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACG8M9_000923"
     CDS             complement(9073..9498)
                     /locus_tag="ACG8M9_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0377713.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACG8M9_000924"
     CDS             complement(9565..9834)
                     /locus_tag="ACG8M9_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0377714.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACG8M9_000925"
     CDS             complement(9845..11440)
                     /locus_tag="ACG8M9_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0377715.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG8M9_000926"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG8M9_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0377716.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG8M9_000927"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG8M9_000927"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0377717.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACG8M9_000928"
     CDS             15906..16880
                     /locus_tag="ACG8M9_000928"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0377718.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACG8M9_000929"
     CDS             17050..18450
                     /locus_tag="ACG8M9_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0377719.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACG8M9_000930"
     CDS             complement(18528..19250)
                     /locus_tag="ACG8M9_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0377720.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACG8M9_000931"
     CDS             complement(19329..19835)
                     /locus_tag="ACG8M9_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0377721.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACG8M9_000932"
     CDS             19958..21757
                     /locus_tag="ACG8M9_000932"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0377722.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..>22336)
                     /locus_tag="ACG8M9_000933"
     CDS             complement(21825..>22336)
                     /locus_tag="ACG8M9_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0377723.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6176 a         4872 c         4033 g         7255 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttg
//
LOCUS       DAZKNM010000030        22172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000030 DAZKNM010000000
VERSION     DAZKNM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.030"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..1445
                     /gene="celB"
                     /locus_tag="ACG8M9_000934"
     CDS             93..1445
                     /gene="celB"
                     /locus_tag="ACG8M9_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0377724.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1529..3034)
                     /locus_tag="ACG8M9_000935"
     CDS             complement(1529..3034)
                     /locus_tag="ACG8M9_000935"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0377725.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(3126..3716)
                     /locus_tag="ACG8M9_000936"
     CDS             complement(3126..3716)
                     /locus_tag="ACG8M9_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0377726.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3895..5541
                     /locus_tag="ACG8M9_000937"
     CDS             3895..5541
                     /locus_tag="ACG8M9_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0377727.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            5590..6309
                     /locus_tag="ACG8M9_000938"
     CDS             5590..6309
                     /locus_tag="ACG8M9_000938"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0377728.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            6788..7735
                     /locus_tag="ACG8M9_000939"
     CDS             6788..7735
                     /locus_tag="ACG8M9_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377729.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            7747..8667
                     /locus_tag="ACG8M9_000940"
     CDS             7747..8667
                     /locus_tag="ACG8M9_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377730.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            8708..10165
                     /locus_tag="ACG8M9_000941"
     CDS             8708..10165
                     /locus_tag="ACG8M9_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0377731.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            10510..11124
                     /locus_tag="ACG8M9_000942"
     CDS             10510..11124
                     /locus_tag="ACG8M9_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0377732.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            11117..12841
                     /locus_tag="ACG8M9_000943"
     CDS             11117..12841
                     /locus_tag="ACG8M9_000943"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0377733.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            12838..14289
                     /locus_tag="ACG8M9_000944"
     CDS             12838..14289
                     /locus_tag="ACG8M9_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0377734.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(14762..16915)
                     /locus_tag="ACG8M9_000945"
     CDS             complement(14762..16915)
                     /locus_tag="ACG8M9_000945"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0377735.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(16917..17963)
                     /locus_tag="ACG8M9_000946"
     CDS             complement(16917..17963)
                     /locus_tag="ACG8M9_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0377736.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            18113..19402
                     /locus_tag="ACG8M9_000947"
     CDS             18113..19402
                     /locus_tag="ACG8M9_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0377737.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            19415..22117
                     /locus_tag="ACG8M9_000948"
     CDS             19415..22117
                     /locus_tag="ACG8M9_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0377738.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         7188 a         3908 c         4487 g         6589 t
ORIGIN      
        1 cgtttaaaga aaatttcact caaccaagac caacagtata tccgtattaa acaatatgaa
       61 aaagcgttat atttaggaaa ggagaataac taatgtttga ttttttgcag aaatacctga
      121 tgggacctat ggggaaaatt gcgcaactta aaatcgttcg ggctgtcatg gcagcgggga
      181 tggcttctat tcctttcacg attgtaggct cgatgttttt ggttttaaat attttgccat
      241 taccgttccc atttttagaa ggatttttta atgcaacttt tttcaaagtt agtgacttgt
      301 acatgatcgt caatacaatg acgatgggga ttttatctat ttactttgcc attgtgtttg
      361 cttatgaatt aacgtcaatt gaacgagatg aacaaaaatt aaatgttaat ccgctcacgg
      421 gggcattact atctgttttt gcattcttta tgtgtatccc tgaattgatc atgactgatg
      481 gtaaaatcag cttgattgct tctatcaatg agacagaaac tgtagtaaat ggtattagga
      541 tgggctcttt tgtcgaacgt ttaggaactt caggaatctt tacagctatt attatgtcga
      601 ttattgcggt cgaattgtat tgtatgtgtg tcaagagaaa ttgggtcata aaaatgccag
      661 atgttgttcc gccaggagtt tctcgttctt tcactgctct gatcccaaca tttgtcattg
      721 cgtttgtggt gatgatcatc aatggattat tagttgcact aggaacggat atcttcaaaa
      781 tgattgctat cccatttggg tttgttacgc agttgacgaa tacatggctt ggtgtcatgg
      841 tgatttattt cttgatccat gcattgtgga tcgtagggat ccatggagcg aatatcatca
      901 cttctttctt gaccccgatc gtattagcta atatggcagc taacgcacaa ggcgcaaatt
      961 atccgttggc aggagaattc aacaattctt atgtaaccgt tggtggatca ggtgcgactt
     1021 taggattgat catctttatt gcattcctcg cgaaatctga tcagctgaaa gtgttaggaa
     1081 aagcctcact tgtaccagga ttattcaata tcaatgaacc aatcattttc gggatgccaa
     1141 tcgtctacaa tccctattta gcggttccgt ttttcttagc tccgatggct tctgctacac
     1201 tcgcttattt tgccattcgg ctggaaatcg tcaaaccgat gcttgcacag atgccgtggc
     1261 cttcaccagt cgggatcgga gcatttgttg gtagtggtgg tgattggaaa gctgctgtgt
     1321 tagcagtcat ttgtgcaatc atcgcattca ttatctggtt cccatttatc aaattctatg
     1381 ataataaatt attaaatgaa gaaagagaaa aggcagcaga attagctagt gaaaagttaa
     1441 aatgatttct aagaaaaaag gaactgtgac aaaaaatcac agtccctttt tttcttagaa
     1501 atgggaaacc actagtatat aaataccatt attcttcaat caaccgcttc acgattgcat
     1561 aatccccttc ataaggttca tctacaccat tgattttttg atagggatcc acgccttttc
     1621 ccgcaataac gactgaatct tgcggagcgc tttctgctaa agcgcgtcga atcgcttcag
     1681 gacgatccat ttcataatga acgatcaaat ctggattagt aattgcttca ttgatttctt
     1741 ccgcgatttt tttcggatct tcaaatgctg ggtcatctgc cgtcaaaaag gcgatgtcag
     1801 ctgtttctga tagcactttt ccaaaatcat gtcgacggga gattgctttg tttcctgggc
     1861 tgcctaatac aacaattaca cggccatctg gatgttcatt tttggcaaat ttcagcaatg
     1921 ttttcaagct tagataatta tgtgcatagt ccacatagac atgcgctcca ttggattgga
     1981 tcaattgatc cattcggcct ggtacacgag cttcttttaa tccgtcttgt gcatcttgac
     2041 tggaggcacc tgttagtgct gcggcaatga tcgcactggc tgcattttct tggttgaatc
     2101 ctccagccag caaaatatca taagcagtat cgcagatagc taagcgatct tcatgagaag
     2161 ataaagtaaa accgtgagta cctttatctg aacggatcac ttgataattg ctttcttccg
     2221 aacgaccata agtgatcgtt ggaatctgga atagatcggc tgtttcccta agtaaatcaa
     2281 aatagtcact ttcatgattt aaaacaactg tttttgaatt caagatcaat tgtcgcttgc
     2341 aatagaaata gtcatcaaat gtcggatgtt cgatcggact gatatggtca ggcgagatat
     2401 tcaagaagat tccgacatct aaagtcaagc cgtatacgcg ttgtgttttg taagcttgtg
     2461 aagaaacttc catcactaga tgagtcatac cattttcaac tgcttctgac atcatacgat
     2521 ataaatctaa tgactcaggc gttgtcaaat gggatttgaa atatgttttg ccatctaacg
     2581 ttgtattcat agtagaaagc aatgctgttt ttttatttgt tgtgtgatcc aagattgctt
     2641 ttgtaaagta cgcagcagtt gttttacctt ttgttccagt gaatccgatc actttcaatt
     2701 tattttgcgg atagtcgtaa aaagccatag caagaagcgc cattgcttta cggatatccg
     2761 ttacgatgat tcctactgct gaattttcgt atggttgttc tgatacataa tattgaatac
     2821 cctgttcgat tgctgaatcc aaatattctt ctttgaaatt cattccttta cagaaaaaca
     2881 aagtagaagc atcagctttt cttgaatcat aacttaactg aagaaattca gctttttctg
     2941 gtacatcaag atgccagcct cttgttgaaa caaattcttt taatagattt tcttttaata
     3001 gtaaatgacg gatgtcttct aaagagaagc tcattgattg atccctcgct ttattcaatt
     3061 tcttggttta ttatagcaca ctctttttaa tatatcgttt gtttccgcta tgacaaaatc
     3121 cagctttatt caaattttct tgacacaggg gacaagcgga tgagtatttt cataaacaat
     3181 gaactgactg taatagatag gagaacgaat agtgtctcca acaatccaaa aacataataa
     3241 ttcaaaaaga cgataagact attggtaagt aaaagtcctt gcggcattgg aagccgaaaa
     3301 aatttgtgga aaataatcgg cggtatcgtt ataccgccat ttgaggcccc gactgtgtac
     3361 aatgtgtaaa ccccaagact gaacaaaaga cttcctgaaa tcagtgaaaa gaaatgtgag
     3421 gagatcattt ccacttttgg aacaatctct aaaacaacag gaaaaagcag acttcccgct
     3481 acagtcttaa aaaatactga ttttcctaat aggacagcgg ctagaagcag catcataaga
     3541 ttgatgctcc aaacagtaac tgagcgttgg atagaaaagg ccgtttcaac taaatgtccg
     3601 atggcgccca ctccggcgga agctatatcg tgagggagca agaaccaatt caatccaact
     3661 gtgataaaga aaagacctaa aaatataacg ggtagttttt ttatttcttg tgtcattgat
     3721 atctcctata aatgttagcc ataaaaggct gtatatttct ttactttacc aaaaaaacga
     3781 aaccgaattc aaaaaaacaa ttgttcttat aaaagttata taaaatcgat tgccagtttc
     3841 tgttttacca attaaaatga tagtaatgtt ttgtagaaag ttggaatctg accgatggat
     3901 aatcaaagaa aaccagcatt agatcaatta acaccgcaag aaaaaatggc acgaggctct
     3961 gcttggatga ccgccagtaa tatcatttca agattgctag gcgctgtcta tatcattccc
     4021 tggtatgctt ggatgggcga aaatgctaag gctgccaatg gattattcaa tatgggatac
     4081 aatatatatg cattgtttct actgatctct acagcaggaa tacctgctgc gattgcaaaa
     4141 caaactgcac gctataattc attgaatgaa tatggcactt cacgacgatt atttatccgt
     4201 gcattacaga tgatgggcgg gttaggcctg ttgtttgctt tgttcatgta catcgcctct
     4261 ccttggctag ctcatgcttc tggtggcgga gaagaactga ttccaacgat gcgttctttg
     4321 agtatcgctg tattggtttt tccttgtatg agtgtgatac gagggtattt gcaaggcaat
     4381 caagaaatga tgccttatgc gctgtcgcag attgtagaac aggtggcacg tattttttat
     4441 atgctattgg ctacattcat catcatgaaa gtcatggatg gtgactatgt gacggcggtc
     4501 acgcaatcaa ctttcgcggc atttatcggt atgctagcaa gtattcttgt ccttttgtat
     4561 tttttgaaga aacatcaagc gtatacatcg gcgtttattc actacagcga aaacaaaata
     4621 aatattgcaa cgaaggaatt gttgttagat accgttaaag aagcgattcc gtttattatt
     4681 gtgggttcag gggtaacgat cttcaaatta gtcgatcagt tcacttttat gaagatcatg
     4741 tctgcatcca ccgagtattc caatgcacag ttactggatc ttttttcgat ttttagtgcc
     4801 aatccagata agttgacgat ggtagttatt gcattggcaa catcgatcgc gtctactggg
     4861 ttaccgttga ttacagaagc tgttacgttg aaagatcgag taggtctggc aaaattgaca
     4921 agcggaaatt tacagttgtt ttcatttttt atgtttccgg cgacatttgg tgttatgtta
     4981 cttgcctatc cgctaaatac attattttat acaccggatt ctctaggaag taatgtactg
     5041 atccaagcaa gttttgtagg actcttccta ggactatata tgctagtttc caacatgctg
     5101 caaggaatgt ttgaaaacaa ggcggcaatt aattatttgg ttattgggtt tctagtgaaa
     5161 ctccttcttc aatacccagc cattcgttta tttgaagtat acggcccttt attggcgaca
     5221 atgatcggat ttgctgtgtc ttgtaccttg atcttaaaga gaatccatga agtagcccgc
     5281 tttaaaccaa gttttgtctg gcgcaggaca ttgctgatct ttattcttac cttgattatg
     5341 cttttggctt cgtggatcac aaagatgatt tttggtgctt ttttaaacga agatagcaaa
     5401 ttccagtctc tattgttgat tcttgtagta gcaggtatag gggctttagt gtatggttat
     5461 ttagctttga aaatccgctt ggcagaaaga ctactaggac caggaatagc gcgtttacga
     5521 cataaattaa aaataaaata agtgaatcag aagagctaga ttgatgctct tttgaaatgg
     5581 gggaaacgca tgcgtttaga taagttttta gcagatatgg gacttggcag ccgcaaagaa
     5641 gtcaaaggat taatcaaaaa aggaacgatc gaagtcaatg gacaagtggt gaaaagcgat
     5701 aaagcgcaag taaatgaatt cgaagatgcg gttacttatt taggagaaat cattacgtat
     5761 caaaaagatt tttactacat tttgcataaa cctgcaggag tcatttctgc tactcaggat
     5821 aatcatgatc aaaccgtaat cgatttattg gaagaccagg attatcggga agatcttttt
     5881 ccagtcggcc gattagataa agacacagag ggcctcttga ttttaacgaa tgacggggtg
     5941 tttgcccatc agttactatc acctaaaaaa catgtggaga aagaatatct agctgaggtt
     6001 agcggtataa tgacagaaga agatatccgt ttatttgcag aaggactgat tattgatggc
     6061 gatgagcaga cattgccggc aaaactccaa attgatcaag tagatcaaga gacagaaact
     6121 tcaattatcc gtctgatttt gcatgaagga aagtttcacc aagttaagcg aatggtcaaa
     6181 gcagtaggaa aagaagtctt atatttaaaa agaatccgta tgggaaaatt ttggctgcct
     6241 aaagatttga ataaagggca atatcgaccc atgaccaaag aagagctaga acaagtgaga
     6301 gaaaaataat tgaatggata gctaaaagaa taagcagagt agttgtagcg ccatctgaaa
     6361 agcagacttg tccgtctgac tttcggatgg cttttttgtg aaatttcggc ttatttccgt
     6421 agaagctact tctggaacac cgtttgttcg gaataaggga ctgtgacaag acttttgtta
     6481 cagtccccat tgttccttgt gactcagagt tagatagtaa ggacacgatc tccctaatac
     6541 tcaggccaaa acgctgaatt cacaacctct gataaacggt gttcaatcag ctgaccctcg
     6601 gtattagctc aaaaaacggc aaaatatgag gaactatttt cccgtttttt tctctaatac
     6661 tcaggccaaa acgctgattt cacaacctct cctatacttt gtttcatttt ccctatactt
     6721 tgtttattta ttctcattat tgaaagcgct ataattacgt tgcgaatgaa tcgaaagggg
     6781 tttttagatg aaaaaacgaa aggggttcaa tcattttttt atgaatctat ggcgttataa
     6841 agcacttgtc ctgatggctg taccgggtat gctttggatg atttttttct tttacatacc
     6901 tgtaatggcg aatgtcgtag cattcaaaaa ttttcatatt tcagcagatg ggttcttggc
     6961 tagcttgagg gaaagtcctt gggtgggttg ggaaaatttc aaatttttat tttcttcgga
     7021 tcaagcattt ctcatcacta aaaatacaat tttatataat gtagcattta tccttttgaa
     7081 tctgctgatc tcagtcgtat ttgcaatcat tatgagcgag ttgagaaata aacgactcgt
     7141 taaagtttat caaacgatgt ccttactacc ttattttcta tcttgggtca ttatcagtta
     7201 ctttgtttac gcttttttaa gtcctgacaa aggaatattc aatcagtgga tcactggaca
     7261 tggaggagaa gctgtcaatt ggtataatga accgaaatat tggccgttta tcttggtgtt
     7321 cattggtaca tggaaaggga ttggttataa cagcattatt tactttgcat ctgttatggg
     7381 aatcgatccg acttattatg aagctgctat ggttgatgga gctagcaaat ggcagcagat
     7441 caaacatgtg acgataccac aacttattcc actaatgacg attttaacga ttttagccgt
     7501 aggaaacatt ttccgagctg attttggcct gttttataac attcctagga attccggtgc
     7561 attatatgat gtgactcaag ttttggatac gtatatctac aatggcctga catccacagg
     7621 agattttggt atgacggctg ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt
     7681 gatgatcaca aatacgatcg cccgacgttt tgatagcgat tctgcattat tctaaggagg
     7741 aaaaaaatga aaaaaaagaa agtggagaga gtccaagtcc gctctttcaa taagacggct
     7801 aattttttct ctaatataat gattgcactc tttgctattt cctgtatttt gccatttata
     7861 tttgttatcg ccatctcttt taccgatgag tccatattgt cgactcacgg gtacagtttt
     7921 tggccagaaa agttcagtat atttggttat acgtacttgt tcgaccagat gcaggataaa
     7981 attttccaag cgcttttcgt cactgtactt gttactgttt taggtacact gatcaatagc
     8041 acggctactt ctttatatgc ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt
     8101 tttaccgttt tttgtttgat tacgatgttg ttctcaccag ggatggtggc caattatctt
     8161 gttatgacaa atcttcttca gttaaaagat acgatttggg cattgatctt gccaatggct
     8221 gtcagtccct tcaatattat tgtgatgcgc acattcttta aacgttcggt ctctgattcg
     8281 attattgaat cggcacggat cgatggagct agcgaactgc gaatatttgt tcagatcgtt
     8341 ttaccgttag ctgttccagg tattgcgaca atcagtttat ttgccgcatt gggttactgg
     8401 aatgactggt tcaatgcttt actatacatc caagatgata agctagttcc actacagtat
     8461 cttttgatga aaatccaaag taatattcaa tatctgacac aaaattcagg agcaggaagc
     8521 caattgacag gcggattagc ctcagttcca ggagaatctg ccagaatggc gatcgtagtc
     8581 atctcgacgt tgcctattgc gatcagctat ccatttttcc agaagcattt tgtcaaaggt
     8641 ctaacaatcg gcggagtaaa agaataaaga aacaaaaata taaatcataa aattaggagg
     8701 aagaaaaatg aagttatgga agaaactagc ctttacaggt gtttcagcgt tgttgttggg
     8761 aacattagca gcatgcggag gttctggttc taaagaccag gcagaagcta gcaattcgga
     8821 cactttgcag atgtatcaaa tcggagataa accagataat ttcgatcaat tgatggaagt
     8881 agcaaacaaa cgaatcgaaa aagagatcgg tgtaaaagtc aatatcaatt acattggctg
     8941 gggcgattac gaaaagaaaa tgaacgtcat catttcttcc ggggaaaatt atgatatcgc
     9001 ctttgcgaac aactatgttt ccaatgccca aaaaggagcc tttgctgatt tgactgaatt
     9061 agcgcctaag tacgctgaaa aagcttataa tgatttagat gaagcatata ttaaaggaaa
     9121 tctggtcaat ggaaaattat atgctttccc agtcaatgga aatgtctttg ctcaacaagt
     9181 acttactttt aacaaagctt tactagataa atacgatctt tcaattgatg gaatccaatc
     9241 ttacgctgat gcagaaaaag tcttgaagga attccatgaa aaagaaccaa acacggcagc
     9301 ttttgccatt ggacaaggct tcaaagtcca agggaacttt gattatcctt tagggaatac
     9361 gttaccattt gctattgatc tggatggaga tgattcgaaa atcatcaacc aatacgataa
     9421 tgaaacgtat atcggactgt tacgtacaat gcatagctgg tacaaacaag gactcattcc
     9481 aagtgacgct gcaacgagca ataaggatta tccactagaa ggaaatactt ggctaatgcg
     9541 tggagaaaca caaggaccgt atgattatgg cgatacaatt ttgacaaatg cagcaaaaca
     9601 agaacttgtc tcaaaagcaa ttactgttcc actaaaatct acagctcaag cacaaatggc
     9661 aaattttgtc gtatcaaata cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct
     9721 gcttaacagt gatccagaat tattgaatgg gcttgtttgg gggatcgagg gagaagcatg
     9781 ggaaaaagtc gatggttcag atgataaaat caaattgcta gatggttatc agccaaatac
     9841 acatatgtct gcttggaata caggaaacaa caagattcta tatacacaag agtctattac
     9901 ggatgacatg attgccaaac gtgatcaatc gattgctgat gcagaaactt caccgatttt
     9961 aggattcagc ttcaacactg acagtgtcaa aacagaatta agtaatattt ctaatgtcat
    10021 gaatcaatat ttagatggat tgaatacagg gactgttgat ccagatgaaa cattaccgaa
    10081 attgaaagac gctttggaca aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca
    10141 atatgatgag ttccgacaag aataataatg aataaagaca aaggggttgt gacaaaagac
    10201 gtgtcacaac ctcttgttcc gaataaacgg tgttcaaaag ccagtttctc ggtattaagc
    10261 taaatatatg taaaaatgtg gaaagccata ttcacaaaat ttttcttact actcgaaact
    10321 aagcggctat ttcacaaact cttggtaaac ggtgttcaat cagctaaccc tcggtattcg
    10381 ctcacaaaac gacaaaatat gaggaactat ttttccgttt gtttctctaa taatcaggtc
    10441 aaggtgctga ttgcccaacc tttttttgta taatgagaaa gtataaactt aatgtttgga
    10501 ggagaagata tgttagggaa ggttttggaa gagttattta tccggatctg ggtcattata
    10561 aaactaacct tgtatttctg gatatataca tttgccggcg ggatcatctt tggattaggt
    10621 gcggcatgga aaacagtcaa tgaactattt tatttgtacg gattcgagta taaagaaatc
    10681 acaataaaaa gaggctggaa tatatataaa agaaattttt taagaggcaa tctgctattt
    10741 agtttatttt tatctggtac tgctttattg agttacaatt tatatttatc tgttcaaatc
    10801 aaagggctca tttttttagc tattgatttt attttacttt ttgccttgct ctgcctattt
    10861 gctacttatc aatacagtat gattttagat agtgaatata cgatctctgt tcctaatctg
    10921 ctaaagctaa gttttatttc ggttttttcc agcttttcgt cttttctaaa aataattatt
    10981 ggtagcggta taattttagg cgtgacttgg caattcaaag gattgatcct gtttggtgtc
    11041 attggtcttt tgacggtttg gaatggtacg atgacaacac attggcgtga agaattagat
    11101 aaacagttgg aaagttatga ataagtttcg gattctattt tcaaaaggaa ccttactaaa
    11161 tcgtctgttg cggaaatacg cggttatcat gatcggtgta acgatgactg ctaccgttat
    11221 ttttagtatc catacttggg aacaaaatca aaaacaggca gaaaacatga caagtgatgc
    11281 cgttcaaagc acaagcagaa tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa
    11341 ccagcttgta gggaattccg aaaagatcga aaatgtgacg acctacttaa caaaacccat
    11401 agatcagtat ctgatgtatg tatacgaaca gcaaaacagt accgatgaat tagtttcttt
    11461 cccaaatcaa atcaaagatc tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt
    11521 gttgaatcaa ctaccggagt attatgaatc ttctagagaa aacaaaggtg gggaaataaa
    11581 aaaggggaca ccaaaattac tagacgcctt ttatatcaag ttgcctatca cacaaggagg
    11641 agctgagtca ggaagtattt ttattggttt tcaaaagaaa gaactagata tgattttaga
    11701 aaatctaacg tcattcaatg gactctcttt atacatgatt tctggaacga caaatcgact
    11761 atacacattc catgataata aaatagctaa agaaacgttt gaaagacaag aaatgatcat
    11821 cacagagagt ctgaagaaaa attcacaatt gccgattgac gatctggaaa aaaacaattt
    11881 tgttcaacac caagaactgt ctgatgatta tcgtatatta gctgtactag ataaaaaaag
    11941 tgtaaaaatc gaaacgggaa aaaatcttgc cccgctgatc attggtatca ttattttaga
    12001 caatatactg cttatctttc tttataaaac attcaaacga tattcacagc aagtatctat
    12061 cgtcatgcag gccatggatc aaacagcagc agggaattta gagacgcgga tcgacataac
    12121 aaacacggaa tatgaactga aggaactttc aatcggtatc aacgaaatgt tggatagcat
    12181 caatcagttt gtagaagaca tctacaaatt agaaatcaaa cagcaagacg cacatatgcg
    12241 ggcgcttcaa gcacagatca atccacactt tttatataat acattagaat atatccgaat
    12301 gtatgcaatc agcgaaggaa gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt
    12361 gcttcgaaac aatacgaatc aagagaaaac gatcacttta aaagaagaac tggacttttg
    12421 cgaaaaatat gtctatttgt atcaaatgcg ttatcctaat cgagtagcct atcattttat
    12481 gatcgatcct gatttggaaa agatcgaagt acctaaattt gtgatccagc cattagttga
    12541 aaattatttt aaacacggta ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt
    12601 cttgcaggaa ggcaaacggg taaggatcat catcaaagat aatggaaaag ggatgactga
    12661 aaaacggcta aaacagattg aagaaaaact gtctcatcct aaagtggaac ttcacggttc
    12721 tatcggtctg caaaacgtga atgaacgttt acgtgccagc ttcggcagca gttattatat
    12781 gtcgcttgag aataatgaga caggtggact gaccgtttct attacattca aggagggatg
    12841 agcgtgtatc aagtgttatt ggtagatgat gaatacatga ttttgaatgg gttaaaaaag
    12901 ataattgatt ggcaaagttt aggctttcaa atagtagcaa cagcagagaa tgccaaagaa
    12961 ggactagctg tacttgaaca gcgacagatt gatttagtag taacggatgt gacaatgcca
    13021 gaaataaatg gactggaatt tattgaagcg gctcaaaaag aacgtcataa ttttgaattt
    13081 atgattttat caggatacca agaattcgat tatttgaaag gaggtatgca attaggcgcc
    13141 gtgaattatt tgatgaaacc cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc
    13201 aaaacacggt tagatcagca aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg
    13261 ttattcagtc aatggttgaa tgaagaattg gatgaaataa gtgaagaaga aataatagga
    13321 caaataagtg agaagcagca taggatagta ttgattcaac tggcaagaac tcatgatact
    13381 cttatcaata gatggttgca agaacggcaa caaatctttt actatcaacg aaattacgga
    13441 gaccgtgtat tgatcacgtt gttgttggag gcagatgaag ttgatggctt ctgttgtttt
    13501 gttcaagaaa actttcctga tcaggaatgg ctgatcagta tcggggaaga gacagattct
    13561 ttggacaaaa tccctgaaac attccaacaa gcgaaagaca atctacagtt gcatcaattt
    13621 tatggagata aagacgagca tgttttttac gcagtacaag cagaaacaaa agatcaaacc
    13681 atcgattttt caagtttcag acgcgtgctg caaaataaac aattagaagt cgctcaaaaa
    13741 atgatcactg atttttttga acagtttcag ttagcagcca tgatgccgga agatatacga
    13801 tattccagtt ttttactgtt tatggagatt caacgggaat tgattgatct ggaagatgag
    13861 gaatatttac aaggaatcga aaaaatccat caggcaaaaa cggtgcaaga acttcagcag
    13921 ctacttctgt cattcatcaa aaagcatcag agacagaaaa agtacagtga caatgtggaa
    13981 aaagtaatcg taatcctgca ccagcattat caagaaccac ttacactcaa agaagtgtca
    14041 gaaaacctgc atttaaatgt catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa
    14101 agtttttcag cttatttgaa ccatttacgt atggaaaaag cgaaacaact gcttttgcac
    14161 agtaatcaaa acatcaatga gatagcaagt gagattggct acaacaacac cacgtatttc
    14221 tctaaattat tcaagaaaat cgtcggcaga tcccctaaag agtaccgaga aaacagtgga
    14281 gaatcataaa agaaaaagcc aatgtaatga aataacttat aaaaaattgt ctagaaatct
    14341 aattttgggc tgttcttatt acttgaggca aggcagcttt ttcatgacct ctcgataaac
    14401 ggagttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tggaaagcca
    14461 ttttcacaaa atttttctta atactcgaaa ctaaacagct ttttcacgac ctctccacta
    14521 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac
    14581 tattttgcca tttgtctctc taatactcag gtcagaacgc tgatttcaca acctctcaat
    14641 aaacggtgtt caaaagcttg catctgcggt aataagacca accaaacgaa aaatatgagg
    14701 atctattttt gcttggctgt tcttattact tgatgcagga cagctttttc acaacctctt
    14761 gttaattata gtttgataag gaaaaaggca aatcttttgc ttgatatttc ttagggtgag
    14821 ggatgatccc taattggtaa gagatcgtgc cgcctttcag caaatcttga tgggtaaaat
    14881 ataatttcgt atattctttg tccatccgtt tgattttatg gataaattgt tgctgcggcc
    14941 gattaggcgt tgcttcaatc gtcaattcct ttccatttga caaaagcaaa acagccttgt
    15001 caattaatgg cataccgata acatattcac cggttccagg cgtgacagga taaaatccca
    15061 gactattgaa gatataccaa gcggccatac ttccattgtc ctcatctcca gggtaaccca
    15121 ttggagaatc gtcgaaggtc tgagtcatca actgtttaag cagtggctgt gccatttcag
    15181 gttgtcctat ataactgaac aagtaaggat agtgaaaact tggctggttt gaaatagcta
    15241 attgtccaaa ttctatcgcg gccatctcac tcatttcgtg gatctcaaaa ccatatcctc
    15301 ctgggtcaaa cgttgggggc tgattgcaca gctcaatgag tttttcttta tatctctcac
    15361 tgccaccata ttttccgatc agtcctgaaa aatcgtgata aacagcaaag ctgctttgcc
    15421 aagcacttcc ttctgcataa tcttgtcccc aacgattagc tgaaaatggt tctctgaact
    15481 tgccatcttt atcttttgaa cgcataaatc ctgactcata gtcaaagata ttttgatagt
    15541 tcaatgattg ctgctgatat ttcttcgtca tttcctcttc ttctagtacc ttggcaactt
    15601 ggctaataca aaaatcgcta taagcatagt ctaatgtgtg attgactgat tcgtggtagt
    15661 gattaggaac atagccatat gtcaagtaat cttctgttcc ttggcgtcca taatttggat
    15721 gtttgctttg aaccgtagca cctttttcca ttgccgttag aaattcaggc atcaagtctc
    15781 gtcggatatc ttttacagca gcatctgcaa tgaccgcatc gatcaatgtc ccaggcataa
    15841 gtccacgttc atcaggagac agccattttg gcaaatagcc actttctcta tagctgttca
    15901 aaaaaccttc cagcatttct tcgtattttt ctacagcaat caaagaataa agaggataaa
    15961 ccgttttata cgtatcccaa aacccattat ttgtatagag aacaccaggt tttatagatc
    16021 gactagtcgt atcatagtgg attttttgcc cactaggatc aatttcataa aatgtttgtg
    16081 gaaataaaaa cacccgatat aaattatgat aaaacgtccg caattgttgt tgatcatggt
    16141 gtgtgagctg tatacgatca aaataggact gccattgttc ttgactgtga gcaaggtaag
    16201 tctctttgct ttggttttgt tctagttcca ggttataagc tgcttgttcc gagctgataa
    16261 aagatgtccc aaatcggata gtttgttttt ttgtctgttc aaaatacaac gggatcgagt
    16321 cgctattttt gttggaaaca gctaactcat caattgttaa aggatattca aatctcaata
    16381 taaaataaaa agaaaaatca ggatcttctg aaccagcaaa atgtgtgatt tttccttcca
    16441 ctgtgtaagg atctataaca aagagctgat ggcgtcccgg aaaggataaa agtaaaccgc
    16501 tgtcattttt gctgtaatca atagataaga ttccgccata catgctaggt atcaatgtag
    16561 aacagatgcc ataacgtaat tgacagatcg acagatgagt cgggcaaaat atactttctt
    16621 ctgggcggta agaactttga gcatggaaaa gggtgttctc ctgtagtttt ccattgatcg
    16681 gcgtaaacag catatgactg aaatcaccca tccaaggact tggttgatgg gtaagacggt
    16741 agccttgaaa aacgcggtca tcaggatgaa accaccagct gccgttttga tccgtagttt
    16801 gcggtgcaaa ataattcatc ccaaaaggaa cgccagtata aggctggcag ttcccattag
    16861 agaaatttgg ctgattcgct gttgcatgtc tagtatctat ttgaccgata ttcattttat
    16921 ttctcctttg ttgattgtct tttgatgaat gaaactggaa atttactatc ggatggtcga
    16981 tcttttgtct ctaaccaagt gaccaaagca tctcccgcaa gcttacctaa ctgttcaaaa
    17041 tcttgtgcga tggttgtcag tggcgggtca acaagagctg ctgcttggat atcatcaaat
    17101 ccgataattg aaaaatcttc agggatatga taaccatgct gtctcaagag attcatggtc
    17161 tggatagcga caacatcgtt ttcacaaaca aaagcagtta cttcatactt agtgatataa
    17221 gaaagcaatt catctaatgt cgcgcttttg tcatccagtg aagtgtggaa agtcaaacca
    17281 gctttgtcca atgcttcaag gtaaccaagg taacgctgtc gaacggattg tggatgagtc
    17341 tgcgtaccaa acaaataagc gatttttgaa tgtccctgtt cgatcaaata agaggcagca
    17401 agcgcaccgc cttggaaatt atcggaaaga acggtaggaa aaggtaactc atagattttt
    17461 ttatctaata tgacgacagg gaattctttc aatgaaagct caaaaagtaa gtcaagatgt
    17521 tgttctgtat caagagcata ataaatcaat ccgtcaaatt cattgattag ctcttttgct
    17581 gttttatttt gtagaaagtc aagcgtcgtc atcatgacat caatctgttt atcttgcatc
    17641 acagggttca atcccttgct aaaatcacca acagacaaat cagcgacata tggaagaaga
    17701 aacagaatcc gtcgtgtttt tgtaggagaa gcaagaatct gttccggaga agcggattta
    17761 acgaaactgc ctttcccgcg aacgcgataa ataagtccta attgctctaa ttcagtcaat
    17821 gcacgtttgg aagtgattct gcttacttta taggtacgtg aaagttcttt ttcagttggt
    17881 agttggctgt ctacaggtaa ttcaccagtt aaaatagccg tttgcaaatc attaaatatt
    17941 gtctggtaca atggttgatt catataaaca cctcttaatg atatatcata ttcaaataat
    18001 accataatgg atgaaaaagt aaataaaaag atattttttg aaagcgtaga cataattggc
    18061 tgtatgaagt ataatcttta aagatatatc aaatagaaag aaggttttat tagtggctta
    18121 taaagatata ccaaaatcgg tgcagaaatt tatggaagat atcacacaaa aatgtgggga
    18181 tatccatgcg gactgggcaa agaattttac agcagcattt gccaatactc tgttgacaac
    18241 agtcaaaaag caaaatgacg gaaccacctt tttattgacg ggagacattc cagcgatgtg
    18301 gttaagagac tctactgcac aagttcgtcc ttatttagtg attgcaaaag aagatgagga
    18361 attagctgac atgatcagcg ggttagtcaa aaagcaattt ttttatatca atatcgatcc
    18421 atatgcgaat gccttcaacg aagaagctaa cggtgctggc caccaaacag atcatacaaa
    18481 aatgaacgat tggatctggg aaagaaaata tgaaattgat tctttatgct acccaataca
    18541 gttggcctat cttctttaca aaaatacagg aaaaactgat caattcaatc aatcttttat
    18601 tgaaggtatc aaaaaaatct tggacgtgtt caaaacagag caagatcatg aacactctcc
    18661 ttatacattt gtacgagaca cgacaagaaa agaagatacg ctgatcaatg acgggcgagg
    18721 tgcaaaaaca gcttttacag gaatgacatg gtctggtttc cgtcctagcg atgatacttg
    18781 ccgttatgga tatctggtac catccaatat gtttgcggtg gtcgtgttag gttatgtgga
    18841 agaaattttt caaacgatac tcgatgataa agaaattgta cagcaaacac agaaattgaa
    18901 gaaagagatc caagagggga ttcagaatca cggacgaacg gttaatcaaa aagggcaaac
    18961 gatttatgca tatgaagtag atggaatggg aaacgccaca gtcatggatg acagtaatgt
    19021 gcccaatctg atcgccgcac cttacttggg atattgttct gaacaagagg aagattatct
    19081 gcaaacaaga agaacattgt tgagtaaaga aaatccatat ttctatgaag gaaagtacgc
    19141 aaaaggaatt ggatcctccc atacgccaga aaaatacgtt tggccgattg cattagctat
    19201 ggaaggaatg acaacgtcag acaaatctga aaaagagcgg atattgaatt tacttgtagc
    19261 aactgacgca ggaacacacc ttatgcacga aggattcgat gtgaacaatc ctgaaaatta
    19321 tacgagagaa tggttttctt gggcaaatat gatgttttgc gaattagtca tggattactt
    19381 tgatattaga gtagaaaagt aggaggtatc gtaaatgaaa aagaaagtat atatcatttc
    19441 tcatagccac tgggatcgtg aatggtacat ggcttacgaa cagcatcaca tgcgactgat
    19501 tgaattgata gacgatctac tagagctatt tgaagttgat ccgtccttca acagttttca
    19561 tttggatggg caaacgatta ttttagatga ctatcttcaa gtaaggccag aaaaaagaca
    19621 ggcaatccaa caagcaatca atgaaggaaa attgagaatt ggaccatttt atatcttgca
    19681 agatgatttt cttatcagct cggaatcgaa cgtgagaaat atgctgattg gcatggaaga
    19741 aagccgtaaa tggggaaccc ctgtcatgtt aggctatttt cccgatacat ttggaaatat
    19801 gggacagacg cctcaattaa tgaaacaagc tggtatttca gcagcagcgt ttggccgtgg
    19861 tgtaaaacca attggctttg ataatcaagt tttggaagca gaaaactatt cttcccaata
    19921 ctcagagatg tggtggaaag gacctgatca aacagcaatc tttggtctgc tatttgccaa
    19981 ttggtatagc aacgggaatg agattccagt agaaaaagaa gcagctcttg ccttttggaa
    20041 gcaaaaatta gcggatgcag aacaatatgc ctctacgaac cacttattga tgatgaatgg
    20101 tgttgatcat caaccggtac aaaaagatat atcaaaagca atccatctcg caaatgaact
    20161 gtttccagat tacgagttta ttcattctaa ttttactgac tatctggaag cagttcaaaa
    20221 agatgtgcca gaagacttag gctcagtaga aggagaattg acaagtcaag aaacggatgg
    20281 ctggtatact ttagccaata cagcatctgc tcgggtttac ttaaaacaat ggaataccaa
    20341 ggtccagcga cagctagaaa atattacaga accacttgcg acgatggctt atgaagttag
    20401 tggaaactat ccccatgatc aattagatta tgcttggaag acattaatgc aaaatcatcc
    20461 tcacgacagt atttgcggtt gttcagtcga ttctgtccat cgggaaatga tacctcgttt
    20521 tgaaaaagca gatgaagttg gaaagtatct tgcacaagat tccttagaac agctgaccgc
    20581 agcaatcgat acgaccggat tcccaaaaga cagctttcca tttgtcattg taaatactgc
    20641 aggaatggat aaaactggtg aagcagaaat aaccatcgaa ttagaaagaa aaagattcgc
    20701 tgaaggaata cctgaacaac tatatcaaga gttagaaaat ttgcctaaac gtaagtatca
    20761 tgtagaaaca aaaagtgggg ccactatacc agctatttta tctgaagaaa ctgtccaatt
    20821 tggttatgat ttgccgaaag accgtttccg tgtgccatat atggcaagaa tgatcaaagt
    20881 gactttacca ttagaaaaca tgccagcatt ttcttgggaa acattcgcgc ttgtagaagg
    20941 ggaagcgaaa gcagaagaaa aggaaacaat gattcatcag agtggacgaa tcatcgaaaa
    21001 cggccctcta catttgacga ttgaaaaaaa tggtaccatt acaatggaag atcgcaaaaa
    21061 caaacgaaag ttgaataatc tgcatatttt tgaagatatc ggcgatatag gaaatgagta
    21121 tatctttaaa caacctttct gtgatcagcc aatcctatcc agtaacaaag aaaactctga
    21181 agtaaaagta ttagtagata caccggaaat agcaaaaata agtattctac aggaaatgga
    21241 aatccctgtt tctgcagacg agcgtttaga aaaagaacag cagatggttg tggaatttcg
    21301 ttatcgaaaa gcagaacgat ccaaagaaaa acgaatcctt caaataaaaa caatcatgac
    21361 aatcagaaaa gatagtaaaa aaatcgactt tgaaacgact ctcgacaatc agatgaaaga
    21421 tcatcgctta cgtgtcttgt ttcctacaaa gttacatgtg gaacatcacg aagcagatag
    21481 tatttttgaa gtggtgaagc gcccaaacca tgtgtctaaa agttgggaaa accccacgaa
    21541 tccgcaacat cagcaagcat ttgtcaatat tcatgatgaa gagtacggtg taacagtcgg
    21601 aaactttggt ttgaatgaat atgaagtaac agaagacgga caaatcgcag tgacgctact
    21661 tcgcagtgtg ggagaactag gcgattgggg atattttcca acaccagaag cacagtgtct
    21721 aggtgagcat cggttcaatt acagcattga actacatgga ccagaagaaa aattctctac
    21781 ttatttgcat gcatacgcag cacagattcc tttttccact caacaaatca aacatcatga
    21841 aggaacattg atttcaaaac aacaatattt aacgataaaa agtgaaacgt ttgcaattac
    21901 agctttgaaa cgcagcaagt ttagtgataa agtagtagta agaggtttca atatgtctag
    21961 tcatttagaa aaattggaaa taactaaaga caatggaaaa actgtcatat tgaatctgct
    22021 tgaagaacca acgaagcaag cggttgtccc aataatccaa ccttatgaga ttcggacaat
    22081 cggattcgag gaggaaaacg aatgtatggc ggaatagaag caggcggaac aaaatttgtg
    22141 tgtgcggttt cagatggtgg agaaatttta ga
//
LOCUS       DAZKNM010000031        22170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000031 DAZKNM010000000
VERSION     DAZKNM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.031"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..2075
                     /gene="tdc"
                     /locus_tag="ACG8M9_000949"
     CDS             240..2075
                     /gene="tdc"
                     /locus_tag="ACG8M9_000949"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0377739.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2380..3858
                     /locus_tag="ACG8M9_000950"
     CDS             2380..3858
                     /locus_tag="ACG8M9_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0377740.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3824..5155
                     /locus_tag="ACG8M9_000951"
     CDS             3824..5155
                     /locus_tag="ACG8M9_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0377741.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5269..7821
                     /locus_tag="ACG8M9_000952"
     CDS             5269..7821
                     /locus_tag="ACG8M9_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0377742.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7828..9267)
                     /locus_tag="ACG8M9_000953"
     CDS             complement(7828..9267)
                     /locus_tag="ACG8M9_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377743.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9242..9424
                     /locus_tag="ACG8M9_000954"
     CDS             9242..9424
                     /locus_tag="ACG8M9_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377744.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9613..9975
                     /locus_tag="ACG8M9_000955"
     CDS             9613..9975
                     /locus_tag="ACG8M9_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0377745.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10162..11019)
                     /locus_tag="ACG8M9_000956"
     CDS             complement(10162..11019)
                     /locus_tag="ACG8M9_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0377746.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11139..12050
                     /locus_tag="ACG8M9_000957"
     CDS             11139..12050
                     /locus_tag="ACG8M9_000957"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0377747.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12057..13703
                     /gene="mdcA"
                     /locus_tag="ACG8M9_000958"
     CDS             12057..13703
                     /gene="mdcA"
                     /locus_tag="ACG8M9_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0377748.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13805..14104
                     /locus_tag="ACG8M9_000959"
     CDS             13805..14104
                     /locus_tag="ACG8M9_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0377749.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14094..15770
                     /gene="mdcD"
                     /locus_tag="ACG8M9_000960"
     CDS             14094..15770
                     /gene="mdcD"
                     /locus_tag="ACG8M9_000960"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0377750.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15774..16367
                     /locus_tag="ACG8M9_000961"
     CDS             15774..16367
                     /locus_tag="ACG8M9_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0377751.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16391..17467
                     /locus_tag="ACG8M9_000962"
     CDS             16391..17467
                     /locus_tag="ACG8M9_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0377752.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17492..18361
                     /gene="mdcB"
                     /locus_tag="ACG8M9_000963"
     CDS             17492..18361
                     /gene="mdcB"
                     /locus_tag="ACG8M9_000963"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0377753.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18472..19236
                     /locus_tag="ACG8M9_000964"
     CDS             18472..19236
                     /locus_tag="ACG8M9_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0377754.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19725..21818
                     /locus_tag="ACG8M9_000965"
     CDS             19725..21818
                     /locus_tag="ACG8M9_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0377755.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21843..22145
                     /locus_tag="ACG8M9_000966"
     CDS             21843..22145
                     /locus_tag="ACG8M9_000966"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0377756.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3787 c         4724 g         6482 t
ORIGIN      
        1 tgtttgttcg ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc
       61 gcttttttgc gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc
      121 tacgtggatt tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg
      181 ttacagataa acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa
      241 tgaaagatat ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt
      301 ataaaatgtt gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc
      361 cttcagatat gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc
      421 gcgaccatat gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc
      481 catggcattc agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt
      541 tattagctta caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta
      601 tggcaacttc gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga
      661 cagacggatg gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt
      721 acgcccgttg catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga
      781 aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg
      841 aaagctttac ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa
      901 atatccaaag attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg
      961 cgttagatat ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt
     1021 atcgaatgga tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc
     1081 caattctagg tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag
     1141 ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg
     1201 ttgatgccgc atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg
     1261 tgccgtatgc ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa
     1321 cgattgacaa agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga
     1381 tagatcctca taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata
     1441 aaaatatgag aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg
     1501 ctccagatat gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg
     1561 cagtctggac agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg
     1621 gggcttcaat cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg
     1681 ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt
     1741 gggtattcaa agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa
     1801 tgttcgatcg gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc
     1861 atactacatt tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg
     1921 gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca
     1981 tgactcctta tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag
     2041 caatggaaaa gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa
     2101 caagagacat atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct
     2161 gtttttcata tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc
     2221 gttcgtttca tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac
     2281 tgggttttgt actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga
     2341 aaacaaaaac atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca
     2401 aagcaaatca cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga
     2461 aatattccag atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta
     2521 ttattgtacg cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa
     2581 aaaggtggtc cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc
     2641 tcatggctgt tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct
     2701 ccgttgtttg gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta
     2761 gttgtcattt tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca
     2821 aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc
     2881 ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt
     2941 ttttcatggg acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg
     3001 ccgatcatgt ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa
     3061 aatccagtta aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc
     3121 aatattttga atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat
     3181 aatatcgcgc aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg
     3241 aatctgttta gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca
     3301 ggaccagcaa agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat
     3361 ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt
     3421 atctcaatct ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg
     3481 ctcgtaaatt caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt
     3541 ttgagattgc gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag
     3601 aaaaagagca atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt
     3661 tctgtcggac tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc
     3721 agtgtcattt tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa
     3781 caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat
     3841 gaaacggact ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac
     3901 aggattattt ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt
     3961 ttatttgata tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt
     4021 agcaaatagt acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg
     4081 ggcatttttt gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt
     4141 aacagcaata ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg
     4201 aggtagcgca ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga
     4261 attggaatct tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg
     4321 tataggattg agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg
     4381 acagatcgat gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt
     4441 tttattcagt tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc
     4501 gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat
     4561 aggaacgatt ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag
     4621 tcctttagtg actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct
     4681 aatgattttg gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat
     4741 tttatatcaa acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa
     4801 aggcgtacct ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct
     4861 acattttttt gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag
     4921 ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa
     4981 gaaacggaac actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat
     5041 tgctttcagc ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg
     5101 gtttgtattc ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca
     5161 gatacatttt gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa
     5221 aacttccctg cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac
     5281 agattgacgg tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa
     5341 gaagaacgac agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag
     5401 acaaaacgat ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc
     5461 attgcctctt ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc
     5521 gtagtgatcg tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg
     5581 aatggtttga agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa
     5641 aaaatccctg cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc
     5701 gttccagcag atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg
     5761 acgggagaat cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc
     5821 ggcgaccaga aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt
     5881 gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg
     5941 gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt
     6001 caaggcattc tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg
     6061 gattggcata tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga
     6121 ttgccagctg ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag
     6181 gcgatcatca aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca
     6241 gataagacag gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa
     6301 gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag
     6361 aaaacagaaa atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca
     6421 gccaatctac ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat
     6481 atggcaacga tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa
     6541 gtattatttg ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct
     6601 gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg
     6661 aaacaggaac tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg
     6721 atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc
     6781 tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt
     6841 ggcttaaaac atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa
     6901 gagttgattc agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa
     6961 ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc
     7021 gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt
     7081 agtgaagtaa gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt
     7141 gcaaaagccg taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc
     7201 ttcttgccaa ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc
     7261 ccgctacctt tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta
     7321 tcttatgcac tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat
     7381 ccaaagcaag gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg
     7441 atcatggttc ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc
     7501 attcttctgc aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg
     7561 tccaagccgt cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt
     7621 atccttgctt tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt
     7681 acagtaagtt taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc
     7741 ttagttgtag aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca
     7801 gctagagaat acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa
     7861 acgattgagc tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga
     7921 atctagaggg aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc
     7981 ggttagttcg ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg
     8041 caagaacgcc ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg
     8101 tttgatagaa aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg
     8161 ttcattgatt tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa
     8221 gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc
     8281 aatcaatatc ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga
     8341 tgtttttttt agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag
     8401 gacctctaaa aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt
     8461 gtaattccaa ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata
     8521 aataccttcc gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct
     8581 gttggtatgg atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa
     8641 gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt
     8701 tcttaaaaat tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt
     8761 atgagtgaaa ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag
     8821 aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt
     8881 gattttagtc acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc
     8941 agggacagca actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg
     9001 tttgaaaaaa atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat
     9061 tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag
     9121 gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat
     9181 ctttgtatga ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt
     9241 aatgattttt ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga
     9301 gaaaataaac ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc
     9361 atttactgat gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa
     9421 ataggtgggc aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc
     9481 ggattgaaca tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta
     9541 cttgtattca tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag
     9601 agatggaagc agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat
     9661 ttgtctccag aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa
     9721 agcaaaaaat ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca
     9781 agtgaccata ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt
     9841 ttgcgaggaa aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct
     9901 attgatctac gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt
     9961 aaatcaacgc aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct
    10021 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt
    10081 attttctaag aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat
    10141 tttgtcccag ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat
    10201 cattttgatc aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa
    10261 gggcaaatct ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac
    10321 ttttgggaaa aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc
    10381 atctgtcgtc gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt
    10441 atgacggaag gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa
    10501 ttgcagatgt tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc
    10561 tttcatgata ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg
    10621 atcggtaata tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt
    10681 ttccataaaa attcggacca agttcggaat agaaacagag gctacctcta aatacaatat
    10741 gacggaagta acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt
    10801 gtctaatgtc ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt
    10861 tctgccgcta tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg
    10921 gctcaaggca ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc
    10981 gaatactttt ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac
    11041 ctttttgtta tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga
    11101 tagcagtgga aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc
    11161 ctggacaagg cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac
    11221 gtttagaaaa tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg
    11281 tttttatcca gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa
    11341 acggtattca gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg
    11401 cttgtcgatc tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa
    11461 tgaaagaagc ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg
    11521 aagctgagga aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg
    11581 aaaattgtcc gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg
    11641 cagaagccaa acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc
    11701 actgtctctt gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc
    11761 tatctgatgc tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag
    11821 aagagattcg taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga
    11881 tggacttggc aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt
    11941 tgacaaagct gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag
    12001 accaacatgg tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg
    12061 aaaaaccaaa ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa
    12121 tcagcaactt gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa
    12181 aactgattgc tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt
    12241 ttctttcaga aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca
    12301 tgtctagtat ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga
    12361 tcgatttctc ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca
    12421 caatgaaatt aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt
    12481 tgattccaaa tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca
    12541 caggtttcaa tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta
    12601 tcgtgatcgt tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg
    12661 actgggtaga cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac
    12721 gagatcctca aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa
    12781 tctacgcaga acacggcgta caatctttga atcacggtat tggattcaat acagcagcta
    12841 tcgaattgtt actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga
    12901 actgggtatt gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa
    12961 gcatccatag ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg
    13021 tcttttttgt cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag
    13081 gacaatatgc catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact
    13141 cttctaccgt aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata
    13201 atcctggcgg tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc
    13261 cattagggaa aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa
    13321 aaccagtctt tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca
    13381 atgcaccagt gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg
    13441 cttatttgta caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg
    13501 gtgttacacc tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg
    13561 gcatcgtgaa attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat
    13621 tagctgcaca aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa
    13681 tgaaattcag aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag
    13741 atggaagaag aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag
    13801 atccatggaa caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca
    13861 tgtaggagtg gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga
    13921 atctactgta aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt
    13981 aacgagattt tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc
    14041 tacaccagga gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa
    14101 atagttttgt agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg
    14161 aattgattga tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc
    14221 ctgaaagtga tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag
    14281 taatcgctat ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa
    14341 tcgttgctgc acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag
    14401 tcattgtact agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta
    14461 tttcagaaat cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga
    14521 ttccaggacg tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt
    14581 taatcgcaac aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag
    14641 caggcgttcg agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc
    14701 gtcaacgggt agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca
    14761 aagctgctgt catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag
    14821 cattttattt atcattactt gattctttag atctttcaaa accaatggcc attgacgatt
    14881 acaatgaagc aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag
    14941 gaaatgaacc agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga
    15001 aaaatccaac ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag
    15061 aagccatcgt gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg
    15121 gtgaagttgg tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag
    15181 aagataaaga caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag
    15241 catacggcta taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg
    15301 cttatgcaaa actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta
    15361 tttcaggtgc atttttagca catggattac aatcaagccg cttgattgct ttaaacaata
    15421 aagaaattac cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg
    15481 ttgctgaaat cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt
    15541 atacaaaact aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag
    15601 aacaaaatgt cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata
    15661 caaatgacac gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg
    15721 tcgaaacaaa taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc
    15781 aagcacatga tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat
    15841 gggtcgttct aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag
    15901 aaatactacc gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct
    15961 caaaacatgc cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata
    16021 gacaagagaa aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat
    16081 tatcaggatg taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc
    16141 cggcagtaaa agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat
    16201 tgccaattca ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag
    16261 tgactcggca aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc
    16321 tattgaaaac agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag
    16381 agagaaagag atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt
    16441 atcagctgta acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca
    16501 cagcgcaaca aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc
    16561 aatgtttatt tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg
    16621 ggaatttttg aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc
    16681 ttatctttgg ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt
    16741 tttactgaaa tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct
    16801 gcagatccaa ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa
    16861 aaaatatgta tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt
    16921 ctatcaaaca caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt
    16981 tgttgctagt ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca
    17041 atatttggac agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc
    17101 tgtccgttca ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa
    17161 tacgatccaa agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa
    17221 aatgattgta aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta
    17281 tcgtacgtat ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag
    17341 ttggctgcag ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg
    17401 tgcatttgct ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag
    17461 caagtaatag taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt
    17521 gactcagcaa ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc
    17581 aaaacctgga ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt
    17641 atttcttaag tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg
    17701 gcatcatgct attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga
    17761 aaaggcaatg ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat
    17821 ggggctattg atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc
    17881 gaaaatactt gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa
    17941 aaaaaccact catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga
    18001 agcgatatta gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa
    18061 tgattcatat gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga
    18121 cacatgcgta ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca
    18181 tgctgctagt caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca
    18241 ggaaaaccaa atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct
    18301 gaagatagag gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta
    18361 aataagacaa aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag
    18421 caaggctatc ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa
    18481 atcaattatt tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa
    18541 ggattcgctg tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga
    18601 ggttcatttg gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga
    18661 gattcttatg ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt
    18721 cttgcattag ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa
    18781 agacaaaaga aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa
    18841 aagaaagcac agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa
    18901 gaagaaactc ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag
    18961 aaagttctgc aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt
    19021 caggaagtag gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc
    19081 agatgctgtg attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca
    19141 caacgacagt atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg
    19201 caagccgatt ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt
    19261 gtgacaaaag ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta
    19321 tcggcaataa ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat
    19381 cgctcaaggc tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca
    19441 tctgcgataa taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc
    19501 ttattacttg atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt
    19561 tttgtaataa taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt
    19621 tttgtttgct ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta
    19681 tgtaagcgga accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa
    19741 ttgaaatact tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa
    19801 gtgagttcaa tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt
    19861 tgagcagtaa aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc
    19921 tggaaaagga aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt
    19981 tgaatcggcg cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga
    20041 tccatctaaa caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata
    20101 tgagacggtt acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc
    20161 aaggactggt ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca
    20221 atcagaatct gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc
    20281 aaaaaatctt tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt
    20341 ccaataagtt gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat
    20401 tgatctggat ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata
    20461 cagaaataaa agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat
    20521 tggacaatgt cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg
    20581 ctagagatat ctccaattca atcgagtggg tacaagctca attactaacg atccaattga
    20641 tccaatttgt agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg
    20701 aaaatctcta taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca
    20761 tgaatccatt gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc
    20821 attttgttcc tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt
    20881 ttcttgtcat ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt
    20941 ataaatcagt cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc
    21001 tgaaagaaaa gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat
    21061 tagtagataa attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata
    21121 agccgctttt agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact
    21181 ttttgaaaag aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt
    21241 tattcactca aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct
    21301 atggcaagtt agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc
    21361 agcctatgtt aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta
    21421 cttgggagga atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag
    21481 agcgctatgt cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg
    21541 gcaaacacat ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca
    21601 gcgtagctac gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga
    21661 ttattttttg tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa
    21721 ttgaattgat caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag
    21781 atttcaaaca actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa
    21841 agatgaacaa attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag
    21901 cacagatgtc tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt
    21961 gtgatatttc ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc
    22021 gttcgcagtt cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata
    22081 ttgtttcaaa aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta
    22141 tctaaataca aaaagaaaag agggtaaaaa
//
LOCUS       DAZKNM010000032        22026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000032 DAZKNM010000000
VERSION     DAZKNM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.032"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..286
                     /locus_tag="ACG8M9_000967"
     CDS             <1..286
                     /locus_tag="ACG8M9_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377757.1"
                     /translation="KSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRFI
                     RMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            345..785
                     /locus_tag="ACG8M9_000968"
     CDS             345..785
                     /locus_tag="ACG8M9_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377758.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            797..1465
                     /locus_tag="ACG8M9_000969"
     CDS             797..1465
                     /locus_tag="ACG8M9_000969"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0377759.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1529..2152
                     /locus_tag="ACG8M9_000970"
     CDS             1529..2152
                     /locus_tag="ACG8M9_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0377760.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2208..2594
                     /locus_tag="ACG8M9_000971"
     CDS             2208..2594
                     /locus_tag="ACG8M9_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0377761.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2807..4198
                     /locus_tag="ACG8M9_000972"
     CDS             2807..4198
                     /locus_tag="ACG8M9_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0377762.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4809..5177
                     /locus_tag="ACG8M9_000973"
     CDS             4809..5177
                     /locus_tag="ACG8M9_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0377763.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5357..6298
                     /locus_tag="ACG8M9_000974"
     CDS             5357..6298
                     /locus_tag="ACG8M9_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0377764.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6367..7098
                     /locus_tag="ACG8M9_000975"
     CDS             6367..7098
                     /locus_tag="ACG8M9_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0377765.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7242..9422
                     /locus_tag="ACG8M9_000976"
     CDS             7242..9422
                     /locus_tag="ACG8M9_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0377766.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9442..9924
                     /locus_tag="ACG8M9_000977"
     CDS             9442..9924
                     /locus_tag="ACG8M9_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0377767.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9940..10701
                     /locus_tag="ACG8M9_000978"
     CDS             9940..10701
                     /locus_tag="ACG8M9_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0377768.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10832..12166
                     /gene="murC"
                     /locus_tag="ACG8M9_000979"
     CDS             10832..12166
                     /gene="murC"
                     /locus_tag="ACG8M9_000979"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0377769.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12473..12958
                     /locus_tag="ACG8M9_000980"
     CDS             12473..12958
                     /locus_tag="ACG8M9_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0377770.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12951..13634
                     /locus_tag="ACG8M9_000981"
     CDS             12951..13634
                     /locus_tag="ACG8M9_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0377771.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13760..16405
                     /gene="polA"
                     /locus_tag="ACG8M9_000982"
     CDS             13760..16405
                     /gene="polA"
                     /locus_tag="ACG8M9_000982"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0377772.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16450..17286
                     /gene="mutM"
                     /locus_tag="ACG8M9_000983"
     CDS             16450..17286
                     /gene="mutM"
                     /locus_tag="ACG8M9_000983"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0377773.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17250..17879
                     /gene="coaE"
                     /locus_tag="ACG8M9_000984"
     CDS             17250..17879
                     /gene="coaE"
                     /locus_tag="ACG8M9_000984"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0377774.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17931..18263
                     /locus_tag="ACG8M9_000985"
     CDS             17931..18263
                     /locus_tag="ACG8M9_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0377775.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18575..19066
                     /gene="nrdR"
                     /locus_tag="ACG8M9_000986"
     CDS             18575..19066
                     /gene="nrdR"
                     /locus_tag="ACG8M9_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0377776.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19089..20462
                     /locus_tag="ACG8M9_000987"
     CDS             19089..20462
                     /locus_tag="ACG8M9_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0377777.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20462..21388
                     /gene="dnaI"
                     /locus_tag="ACG8M9_000988"
     CDS             20462..21388
                     /gene="dnaI"
                     /locus_tag="ACG8M9_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0377778.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21517..21957
                     /locus_tag="ACG8M9_000989"
     CDS             21517..21957
                     /locus_tag="ACG8M9_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0377779.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7471 a         3813 c         4807 g         5935 t
ORIGIN      
        1 aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt
       61 gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat
      121 ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga
      181 cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag
      241 aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt
      301 ggattttttt gtcataataa atattagatg atggaggaga taatatgatg caaacatggt
      361 tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga
      421 aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc
      481 gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat
      541 ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat
      601 atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg
      661 atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta
      721 aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg
      781 tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt
      841 tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc
      901 agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc
      961 tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg
     1021 gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa
     1081 aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg
     1141 atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt
     1201 ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc
     1261 cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg
     1321 tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat
     1381 ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac
     1441 aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa
     1501 agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac
     1561 attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg
     1621 tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt
     1681 gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa
     1741 agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa
     1801 agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct
     1861 gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt
     1921 tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag
     1981 aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg
     2041 agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt
     2101 ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct
     2161 ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa
     2221 gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag
     2281 aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag
     2341 aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat
     2401 ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg
     2461 tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt
     2521 cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag
     2581 aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt
     2641 ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat
     2701 aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt
     2761 tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat
     2821 cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa
     2881 gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg
     2941 cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg
     3001 gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt
     3061 gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat
     3121 tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat
     3181 tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac
     3241 agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca
     3301 gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac
     3361 aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa
     3421 aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat
     3481 cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat
     3541 gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt
     3601 tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc
     3661 aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac
     3721 ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg
     3781 agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg
     3841 ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa
     3901 tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg
     3961 tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa
     4021 cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg
     4081 tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa
     4141 agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc
     4201 aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat
     4261 agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg
     4321 tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
     4381 tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac
     4441 cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa
     4501 aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa
     4561 caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca
     4621 tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt
     4681 atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat
     4741 tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga
     4801 ggaaacagat gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa
     4861 atgcaacgtt ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga
     4921 cgcttataat tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag
     4981 catttgttga gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca
     5041 gtaaagatat ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata
     5101 tggtacagct tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg
     5161 cggttacagg taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga
     5221 agtcttttta attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata
     5281 cacaagcaaa taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga
     5341 aggaaggaag aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga
     5401 agaaccagag gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg
     5461 tcgtattcta gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac
     5521 gactgcctat atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat
     5581 cgatccaagc gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt
     5641 aagtttaaag ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc
     5701 gcaagaattt aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga
     5761 ttatgaatat gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg
     5821 agagcttcca gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac
     5881 ctactgtact ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt
     5941 caaagatgtc ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca
     6001 aggagaatta tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa
     6061 ccaagtagat aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata
     6121 tatcaattgt agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga
     6181 aaaacattta ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca
     6241 agaccatgga atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa
     6301 aacaatagtt ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg
     6361 atccacatga aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt
     6421 gaaaaactta ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc
     6481 aatgatcgag gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa
     6541 acaggagcca tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca
     6601 ccggaggact ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg
     6661 attcacccag gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg
     6721 agatattcag aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat
     6781 tatctttttg tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta
     6841 agagacttta tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca
     6901 attgtatttg gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc
     6961 tggattcagg atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat
     7021 ggggttgttt ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga
     7081 ccttggcatg gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct
     7141 ttttattttt atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga
     7201 tagaataaag aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc
     7261 agacgatcat tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta
     7321 ctgtacttaa tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag
     7381 aaatgactgg aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc
     7441 ttgaaaactt agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac
     7501 tgactcccga gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt
     7561 tgtatcgtcc atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac
     7621 ttgaaccact cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag
     7681 aaaaatatat tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc
     7741 atgagattat tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt
     7801 atacgtataa caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag
     7861 gcgtatatga aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa
     7921 tattagctac taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg
     7981 aagcagcaat tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt
     8041 cttcattcgt cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg
     8101 agcgagaact gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg
     8161 gcgaaaacct ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat
     8221 ttgatccggc ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt
     8281 tagcaatcga agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg
     8341 gaccagcttt tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg
     8401 gtacagccag tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata
     8461 aaacctatta cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg
     8521 cacgaaaaga atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc
     8581 gcctgcaaga tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc
     8641 agtaccaaca tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa
     8701 cagcggttaa ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata
     8761 tatcaggatt gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag
     8821 aattcaccgc acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac
     8881 aagcaatcgg attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc
     8941 atcccgaaag ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag
     9001 agcttggtac agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg
     9061 aaagcctatc agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg
     9121 gacgagatat gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg
     9181 aagacttgaa accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg
     9241 catttgttga tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa
     9301 aattcgtcaa gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag
     9361 aacaagtcga tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt
     9421 aacaatgagg agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata
     9481 tcattaaaaa gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt
     9541 acaactggcg ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt
     9601 gaagagtcag acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat
     9661 cttcatctag ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa
     9721 aaaacaggag ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat
     9781 tatcgatgcc aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa
     9841 tatcgttgtg gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt
     9901 aatgaagcac aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg
     9961 aatctaaaag actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt
    10021 gaggaactga cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat
    10081 aaagcagcac aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg
    10141 gggcatgcga ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat
    10201 ggcatgagtg aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat
    10261 aaggcttttc tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg
    10321 gagattcttc attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca
    10381 ctgcaacgca ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc
    10441 gcgaatgctg tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca
    10501 gcaaaagaac tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa
    10561 atggaacgtc tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct
    10621 cacattgata tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa
    10681 acggattttt ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg
    10741 gttgaaattt tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag
    10801 aatggacgct agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt
    10861 tgttggtatc aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct
    10921 aaatgtccaa ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc
    10981 aaatatcacg attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc
    11041 aggaaatgca tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga
    11101 agtgattcgc tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt
    11161 aacaggctct cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt
    11221 ccgtccaaca agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt
    11281 cttttcattt gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc
    11341 aatcatgacc aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt
    11401 tacagctttc caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga
    11461 tgcttattta cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa
    11521 cgatgatatc caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta
    11581 tcatggggat gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt
    11641 aaatgcttta ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc
    11701 agaagaaatg ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga
    11761 catgaccgta gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg
    11821 cgcacgccaa aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac
    11881 gcgtacgatt gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta
    11941 cttatgcgat atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct
    12001 tggagcgaaa atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt
    12061 agattatcat gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca
    12121 agcgtatgaa aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag
    12181 tgtccttata aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg
    12241 caacttatga taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca
    12301 aaaacatgag aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt
    12361 cacgacctct gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg
    12421 ctacttttgg tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat
    12481 aggtaagccg agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc
    12541 cttaacagaa tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc
    12601 aaatcctttg tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt
    12661 tccatcagtg atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc
    12721 aggatcacat gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac
    12781 actgactttt cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc
    12841 tgtagaagcg gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc
    12901 gccacaagtt cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc
    12961 caagtacatc aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta
    13021 tgggacttgc ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat
    13081 tagcaacttt tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga
    13141 ttttagtaat ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat
    13201 ttattgccta ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata
    13261 ttggaacagt aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg
    13321 tcggagtaac aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa
    13381 tcggagtgat cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc
    13441 tgttcatctc cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa
    13501 gcatccgtaa tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca
    13561 tggcattgaa gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg
    13621 gacggaacaa ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta
    13681 tggtcccgct ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt
    13741 acagtaagga gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt
    13801 ggcttttcgc gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg
    13861 tcttcataca aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga
    13921 gcaaccgact catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat
    13981 gtatgcggag tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc
    14041 atatatacgt gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga
    14101 agcagatgat attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt
    14161 cgtgctttca ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat
    14221 cactgtaaaa ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata
    14281 tgatgggttg acgccaaaac agattattga catgaaggga ctagccggag atgcctctga
    14341 caatatcccc ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata
    14401 tggctcagtc gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga
    14461 aaatctgata aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac
    14521 ttcagcgcct gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa
    14581 actcgttccg ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt
    14641 agaagaagaa gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga
    14701 aatgtttact acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga
    14761 ggaaatcgtc ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc
    14821 tgtattcgaa aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt
    14881 atatgatgcg aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat
    14941 cgattttgat gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat
    15001 cgaaggaata gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg
    15061 caaaggggcg aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg
    15121 aaaagtagct gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa
    15181 ccaagaagat cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga
    15241 aataagcggg atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc
    15301 tcgcttaaga gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa
    15361 ttcaccaaag caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa
    15421 aacaaaaact ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc
    15481 aatcgtagag gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga
    15541 gggactgctg aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt
    15601 gacccaaaca ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt
    15661 agatgaaggg agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta
    15721 ttcttctgac tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca
    15781 tttgaaagct gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt
    15841 tggaatcgaa aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa
    15901 ttttggtatc gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg
    15961 aaaagcagca cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata
    16021 tatggaaaga atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg
    16081 ccgtcgatat ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg
    16141 aacagctatc aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat
    16201 cgaattggat aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca
    16261 tgatgaactt gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa
    16321 ggaagttatg gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg
    16381 aaaaacgtgg tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga
    16441 aaagaaaaaa tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta
    16501 gtagtaggaa aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca
    16561 gagacaccca tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga
    16621 gggaagtttt taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa
    16681 ggaaaatatc aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc
    16741 ttttttgagg atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca
    16801 atcgtcgaaa aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct
    16861 ttgccagata gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca
    16921 atcaagccat tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat
    16981 gaagcattat gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa
    17041 gaggtagaac aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga
    17101 ggtaccacga ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca
    17161 ctgcatgtct atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa
    17221 acaaaagtgg cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca
    17281 aagtaggatt tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca
    17341 aagtctttca gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg
    17401 ttgagcctgg aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc
    17461 agcctgatgg gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa
    17521 aacggcaaaa acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa
    17581 tcgcctctct aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag
    17641 gtcattatga ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc
    17701 ttcagcgttt gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta
    17761 gtcaacttcc aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa
    17821 caaaagaaaa acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag
    17881 tatctgtgtc aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat
    17941 taaaaaatca aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca
    18001 ataaagaaaa tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa
    18061 agcagtacca agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc
    18121 ggcaagattt gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc
    18181 cagaaagtga ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata
    18241 caggaatcga agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga
    18301 gaaggtgttc atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag
    18361 ttatttctca gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg
    18421 acacaattac gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac
    18481 agtcgcgttt gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc
    18541 tttatataaa atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa
    18601 taattcacga gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg
    18661 cgaatgtgag aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt
    18721 attagtaatc aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg
    18781 actcatccgt tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa
    18841 ccatgtcgaa aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg
    18901 tgaatatgtg atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag
    18961 tgtttatcgc cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga
    19021 taaagcaaaa gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag
    19081 gcatttcctt ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa
    19141 aagcggctcc gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa
    19201 tcggcggaga agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg
    19261 gcaaaggtcc tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc
    19321 aattttatga tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag
    19381 aagatagtga tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag
    19441 catttttcaa agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat
    19501 tccagaagct tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa
    19561 tcacaaaaaa atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag
    19621 aaaacatcga atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag
    19681 cagattcgac attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa
    19741 gtcttgatga tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg
    19801 aattgacact tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg
    19861 aaaaagaact gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag
    19921 aaacagttgc ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg
    19981 cagcgcttca gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa
    20041 tcaagtcaga aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg
    20101 tttctcaatc aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga
    20161 tcaagaataa tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac
    20221 aaaagaaaat cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag
    20281 caaaacaatc aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac
    20341 gggaaaagct tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga
    20401 aaaaagcaga gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct
    20461 agtggaagac gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa
    20521 gtatgctgaa atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc
    20581 acaccaagaa gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt
    20641 cgtgcaagaa aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga
    20701 gccgaagctg acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt
    20761 gttagcacgg caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa
    20821 agatattcaa tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag
    20881 cttagaagcc ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg
    20941 actgtatcta gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag
    21001 agaattagcg attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga
    21061 gatgaaacag gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc
    21121 acctattttg atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga
    21181 agtattcggt gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc
    21241 gaattttaca atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga
    21301 gccgctaaaa gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat
    21361 gactggtcgc aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta
    21421 gtagtaaaag caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc
    21481 aacaagtaat gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata
    21541 tctgtagttg gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt
    21601 tctgttgatt cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga
    21661 caaagcatat ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg
    21721 cttcagttga aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca
    21781 ttcagcaatg acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat
    21841 gaattgaaaa tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg
    21901 tctatcccac cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca
    21961 ataacatttt ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta
    22021 aggaga
//
LOCUS       DAZKNM010000033        21992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000033 DAZKNM010000000
VERSION     DAZKNM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.033"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..469)
                     /locus_tag="ACG8M9_000990"
     CDS             complement(59..469)
                     /locus_tag="ACG8M9_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0377780.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            744..956
                     /locus_tag="ACG8M9_000991"
     CDS             744..956
                     /locus_tag="ACG8M9_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0377781.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            959..1171
                     /locus_tag="ACG8M9_000992"
     CDS             959..1171
                     /locus_tag="ACG8M9_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377782.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1311..1466
                     /locus_tag="ACG8M9_000993"
     CDS             1311..1466
                     /locus_tag="ACG8M9_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377783.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1463..1687)
                     /locus_tag="ACG8M9_000994"
     CDS             complement(1463..1687)
                     /locus_tag="ACG8M9_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0377784.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1765..1953
                     /locus_tag="ACG8M9_000995"
     CDS             1765..1953
                     /locus_tag="ACG8M9_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377785.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            1950..2123
                     /locus_tag="ACG8M9_000996"
     CDS             1950..2123
                     /locus_tag="ACG8M9_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377786.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2092..2220
                     /locus_tag="ACG8M9_000997"
     CDS             2092..2220
                     /locus_tag="ACG8M9_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377787.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2279..2614
                     /locus_tag="ACG8M9_000998"
     CDS             2279..2614
                     /locus_tag="ACG8M9_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377788.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2847..3788
                     /locus_tag="ACG8M9_000999"
     CDS             2847..3788
                     /locus_tag="ACG8M9_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0377789.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            3790..4680
                     /locus_tag="ACG8M9_001000"
     CDS             3790..4680
                     /locus_tag="ACG8M9_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0377790.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4723..5523
                     /locus_tag="ACG8M9_001001"
     CDS             4723..5523
                     /locus_tag="ACG8M9_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0377791.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5538..6389
                     /locus_tag="ACG8M9_001002"
     CDS             5538..6389
                     /locus_tag="ACG8M9_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0377792.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6590..7051
                     /locus_tag="ACG8M9_001003"
     CDS             6590..7051
                     /locus_tag="ACG8M9_001003"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0377793.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7077..7529
                     /locus_tag="ACG8M9_001004"
     CDS             7077..7529
                     /locus_tag="ACG8M9_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377794.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            7635..7829
                     /locus_tag="ACG8M9_001005"
     CDS             7635..7829
                     /locus_tag="ACG8M9_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377795.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            7860..8153
                     /locus_tag="ACG8M9_001006"
     CDS             7860..8153
                     /locus_tag="ACG8M9_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377796.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8084..8623
                     /locus_tag="ACG8M9_001007"
     CDS             8084..8623
                     /locus_tag="ACG8M9_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0377797.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            8698..9165
                     /locus_tag="ACG8M9_001008"
     CDS             8698..9165
                     /locus_tag="ACG8M9_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0377798.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            9912..10142
                     /locus_tag="ACG8M9_001009"
     CDS             9912..10142
                     /locus_tag="ACG8M9_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377799.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10301..11134
                     /gene="terS"
                     /locus_tag="ACG8M9_001010"
     CDS             10301..11134
                     /gene="terS"
                     /locus_tag="ACG8M9_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0377800.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11127..12542
                     /locus_tag="ACG8M9_001011"
     CDS             11127..12542
                     /locus_tag="ACG8M9_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0377801.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12554..14083
                     /locus_tag="ACG8M9_001012"
     CDS             12554..14083
                     /locus_tag="ACG8M9_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0377802.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14001..15053
                     /locus_tag="ACG8M9_001013"
     CDS             14001..15053
                     /locus_tag="ACG8M9_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0377803.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15055..15372
                     /locus_tag="ACG8M9_001014"
     CDS             15055..15372
                     /locus_tag="ACG8M9_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377804.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15499..16122
                     /locus_tag="ACG8M9_001015"
     CDS             15499..16122
                     /locus_tag="ACG8M9_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0377805.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16137..17027
                     /locus_tag="ACG8M9_001016"
     CDS             16137..17027
                     /locus_tag="ACG8M9_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0377806.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17050..17265
                     /locus_tag="ACG8M9_001017"
     CDS             17050..17265
                     /locus_tag="ACG8M9_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377807.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17277..17618
                     /locus_tag="ACG8M9_001018"
     CDS             17277..17618
                     /locus_tag="ACG8M9_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377808.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17618..17989
                     /locus_tag="ACG8M9_001019"
     CDS             17618..17989
                     /locus_tag="ACG8M9_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377809.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            17982..18377
                     /locus_tag="ACG8M9_001020"
     CDS             17982..18377
                     /locus_tag="ACG8M9_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0377810.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18379..18756
                     /locus_tag="ACG8M9_001021"
     CDS             18379..18756
                     /locus_tag="ACG8M9_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0377811.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18757..19365
                     /locus_tag="ACG8M9_001022"
     CDS             18757..19365
                     /locus_tag="ACG8M9_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0377812.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19365..19706
                     /locus_tag="ACG8M9_001023"
     CDS             19365..19706
                     /locus_tag="ACG8M9_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377813.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            19948..>21992
                     /locus_tag="ACG8M9_001024"
     CDS             19948..>21992
                     /locus_tag="ACG8M9_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0377814.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWI"
BASE COUNT         7913 a         3220 c         4554 g         6305 t
ORIGIN      
        1 ataatttttc tatttcttct aatatccagt atttgtttaa atgcatacca aagcccacct
       61 attgacgata ttttttagga gtgaattttt tctttgcttt aactttggtt aaggcaagtg
      121 caagttgaat ggcatcgtat aatttttcct tatcttcatc gtctaaaggt tcaccataat
      181 aatttgtttc agcatctgaa ttcaagcctt caagtaattt ttcagcttgt aaagcaatat
      241 cttgtttatc tttttctgtt aaatcataat aatggcgttt atcagtacgt cctaaaagat
      301 aatcagttga tacattaaag taatctgcaa ctttttgtat tttatctcca gatggagtac
      361 ttttattcca tctagacatg cttccatttc cgaaatttaa ctttctttca agttctgcca
      421 acgtcattga acgttctttt aataagattt ttatacggtc taatagcata ggatatgcat
      481 tcctttcaaa ataccgacaa aaaaagtatt tttctgcaaa ttatctattt acaaatagag
      541 aatttgcagt tatactagtt ttgtaagcaa gatatttacg gcatacaaag actaaaagca
      601 ataaactaat agtctcccca gacatcagta agctcgcttt ttgtgcgctg tattttgtat
      661 gcttacataa tagcatattt gcagtttttg tcaaacttat tagacaaaaa tattagaaaa
      721 tattcgataa gggggtatga agcatgactt taaaacagca agtattaatt gctttagtaa
      781 agaaaggttg gagtcaaaga gagttagctc gacgaatggg tatatcaatt acttatttac
      841 gagatatctt catcgaaaaa agaaaaccta aggaacgttt aaaacaaatc gaagaacttt
      901 tggatatcaa gttagaagta gattctaatg atcagccatc agaacaggag gcgtaaaaat
      961 ggtacaacgt ttgagcaaag cggagtttaa agagaaatat aagtattctg aaagtactta
     1021 tcaaagaaga atgaaggaat ttaaaaaatc acgttttagt gaggggtata acgcagttac
     1081 ttccaaagaa ataacaattg atgtcgattt atatgaacag tttagaacat ggcgttctaa
     1141 aaatcgattt agagttagag ctgtttgtta atagctaaaa aatctaaatt caccacttct
     1201 gaaaagagga gttgctgctc tcctttcaag tttgccaaag tccacgtttt ttcattttgg
     1261 caactcctct tcttggaggt ggtgacggcg agagacgaag gagggatgac atgaaggaag
     1321 aatcaaaaat agggattttg aatgagaaca attacacaag attaattatt gaaacagaag
     1381 aagggaaaaa agtagcagaa gttacattga ccgatgcaac tcctgctgac ggttatcgta
     1441 ttcgattaac tcctaaatat gattaacctt ttggtgggtg tggatcgttt ccgtgactat
     1501 cacggctttg aatttttcca tctctgccat gaataaatag ctcagatcct tgatttttag
     1561 aaatctctct agcgattttt actgcttctg attttgtatt agtatgtgca gtcgctttag
     1621 aatttccagc acctttgaca ttccaaccac cattggaatt aggtacaaca tgttgatttt
     1681 ttcccatagt tttcacctcc atttctatag aaatatttta tcaaatagaa atggagaaat
     1741 tcaataaacg aaaggagaag tgatatggca tataccttgc aacaagaaca tcaaattctc
     1801 ggtttgatta aacaacgcag gaaacaatta caagatgacc gtgcagcgct tagaaaagcc
     1861 gatgagctat cagataggca agctgaacta attgcttctg aacttgagga tttgagaatg
     1921 ctagaaataa aaaataggga gattagatta tgaagaagac agacacactt tttataggat
     1981 tcattttggg gttattagtg attgtagcgc accaaagtat tatcggggga agcttgttcg
     2041 cagcattgat ggttttaatc aatctgcttg attcaaaaga aaggagcaac tatggcacga
     2101 gaagaagcgc taaaaatcgg taaagtgatt gctgataatc ggtggggaaa tagccgccct
     2161 attattttaa gcaagcaaca tatcgaaaag caaaaagcat ggcaacaaat aaaaaagtga
     2221 ctctgccagc cagcaaagag tcacatacaa atttataaaa ctaggagaat tatatcacat
     2281 gaataagaaa attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa agcaaggtgt
     2341 ttctattatt tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt atggtgagac
     2401 aacagaaatt ttactttgtc ttgctatgca agaagaacat ttagatgaaa atctcccatt
     2461 gtcagcacat attatgcgta gaattgctgt ggatgcttac gaacaagcga aaaatgaaga
     2521 agaaaatcaa ccttcaaatc atacttttgt tattaataac aaggaagatt tagctgatgt
     2581 gatgactcgt attctcaagg gggaatttca atgatgaatg aaaaaacaat tgatctactc
     2641 attattttat ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt tggcttgtta
     2701 ttcgcaatta ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt taaacatgaa
     2761 cgagttagca aatcttgaca actatttaac tgatcctgat tatgctaaac caccatatga
     2821 agcaccaatt gatgaggagg atgaagatga gtaaatctac cttagaaatg agccatcaag
     2881 aatggcttga agaccgtaag aaaggcatcg gaggttcgga tgttggaaca attttaggat
     2941 tgaataaatg gaaatctcct tatcaactat ggctagaaaa aacaggacaa gtagtacttg
     3001 aagaatcagg aagtgagccc gcttattggg gtaatgtttt agaagaagtg gttgctaaag
     3061 agtttcagga acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt gaacatccat
     3121 tacatccatt tttaagagca aatattgatc gggaagtagt gggagaaaat gccattctgg
     3181 aatgcaaaac agccaatcaa tttctcggta aagaatggga aggcgaagag gtaccgctta
     3241 gctatctctg tcaagttcaa cattacatga acgttctaaa caaagactat tgttacatcg
     3301 ctgtcttaat cggtggtcaa aaatttatct ggaagcggat tgaacgagat caagagctga
     3361 tcgatacaat cactgaacaa ttagtagagt tttgggaaac aaacgttctt ggaggtatcg
     3421 agccaattat tgatggtagt caagcgacag ctgatttctt gaaagagaag tatgcagatg
     3481 ttgaagacgt tcaaacgacg ttaccaattc atttcgatga actagttgaa cagaaaaacg
     3541 aactcaaacg gactaagaaa gaaattgaat cagctatccg tcaagtagac aacgagatta
     3601 tcagcgagct aggtaaacgt gaagccagta tcggtatcac tcaaaaaaac atcatcagtt
     3661 ggaaatttgt ccgtacgaga cgtatgaact cgaagaaact agcagagaaa tatccagatg
     3721 tcgcaaatga tgaagagatt tataacgtta ctgaatcaag aaggctaacc gaaagggaga
     3781 tcaaataata tggcaacaaa tgaatcgtta aaaaatcaat tggcagcaaa gccacagaaa
     3841 caagttgcac caggtcaatt aggacttaaa gctttgatga atacaccaac aatgagaaag
     3901 aagttcgaag aggtgcttca tgacaatgct aatgctttta tgtcgaatgt tatgactctt
     3961 gtatctaatg acagttatct tgcagatagt gaaccgatgt ctatcatgag tggtgcgtta
     4021 actgctgcaa cattaaatct tgggctagat aagaatttag gttatgcata tttagttcca
     4081 ttcaatagta aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt catgcttggc
     4141 tataaaggat atatccaatt agcccaacga tcaggtaaat acaaagcatt aaatgtgatc
     4201 gaagtttacg aaggagaact aaaaagctgg aaccgactga cagaagagtt tgagtttgat
     4261 ccaaatggta gaacgtctga tgaagtcatt ggatatgttg gctatttcga gttactgaat
     4321 ggattcaaaa aaactgtcta ttggaccaaa caagaaatcg aagctcatcg gattgctaac
     4381 aataaagatc gcgataagac aaagttaagt ggtgtgtggg catctgatta caatgcaatg
     4441 gcacgaaaaa ctgttttgag aaatcttctt tctaaatggg gaatcttgtc catcgaaatg
     4501 caagaagcca ccacatcgga tgagagagtc caaagggttc aagaagacgg cagcattatt
     4561 gctgaaacag aagttgaaga agatattcct gaaagaaaag aagcagaggt tatatctgaa
     4621 gaaaatgaag atgtacaaac tggattattt gatgcatcta atccgccgtt aaacaaataa
     4681 tgagggagct ttctcccttg cttttctaga ggagaaatac gaatgaacac aggatatgtc
     4741 aaattacatc gaaaagtgat gaattcattc gtttggacca atccttatat gtataaatta
     4801 tggaacttgt gtttgatgaa agcgagtcat gagaatcgca agatactttt taatggaaaa
     4861 gagatacaag tgaacagcgg agaattcgtc acagggcgcg atgctatcac atctgagatg
     4921 aacgaaggcg tgaaacgtga acatcaagtg aacagcggtt ctgtatggag atggctaaaa
     4981 caatttgaaa aaaacggaat gttgaacatc aaatcaacta cgaaatacag cgttatatca
     5041 ataaaaaact ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa acgcacaaca
     5101 agtgaacaac aagtgcacac aaacaagaat gaaaagaatt ataagaatga aaagaataat
     5161 aaaacatcat cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc agtttactac
     5221 attctcgcgg aggagttatt caaacagatt tgtcagaatc aggaaatcaa aaagccgaat
     5281 ctgcaaagtt gggcagatga cattcggaaa atgatcgaga tcgacaagcg gaccgaaaac
     5341 caggtacggg gaatgattga atggagtcag cacaatgtgt tttgggcatc gaatatttta
     5401 tctgctaaaa aattgcgaga aaaatacgac acaatggcag cgcaagcaaa tcgtgattat
     5461 aaaacaaaac aaactaaaac gcttgaatac gagaaattta gcacagatga gttgcctatt
     5521 tgagaggagg cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg atacagaaag
     5581 tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga tggcgtacga
     5641 agaaccctga tggctcagaa cgttgtaagc caacctgtat gcaatgtggg tataaagcat
     5701 tgcgagttca ggaagactta caaacagaac gaatttacaa cgagagcctg aaagcaagag
     5761 caatcaattt tttcaaaggt ggttctgttg tgcctaatca agcgttgttt gattgcacat
     5821 tgcagaatta tcaaattgtc gatcaagaaa caagacaagc ggttgaagta accaaacgct
     5881 ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt tttaactgga aaacaaggaa
     5941 caggtaaaag tcacctagcg atggcagcgg cttgggaagt cttgaagcga tcaaactacg
     6001 ataaaaaaat cttatttatc gggttacagg aaatgctgga tcaaatcaaa ttttcgtaca
     6061 acaatcctga actcagaaaa acgattgagg gatcgttgat tgcagatatc aaaacagcgg
     6121 atttagtcat cattgatgat atcggttcag aactaggaaa agatgcatca gatagtcgag
     6181 cgtttggcat aaacacgcta aattcgttct tggatgcacg acagaaccta gcaacgatta
     6241 tcacaacgaa cttgcttggt gaagaactga aaaaagctta cggtacgaga acaatatcaa
     6301 gaatgtttgt caactctgat ggatttacga tggtattttc tcaaacagca gacaagcgca
     6361 taaaaccagt caaaggagcg atcgcatgaa tttgactaaa caagaaaaaa gaatctgtcg
     6421 tcagataatg ttgcaaatta taggtgatcg tgacatggac gaattatctc aacaagagag
     6481 tcgaatggtt cggaagataa gtaagctaat tatttatcgg gatacagaaa gatttctcac
     6541 tgttttaggt aacgtctacg agaatccaga gttattggag gtagagtaat tgccaaaaat
     6601 attagatgca tgttgcggta gtagattgtt ctggtttgat aagaacaatt ctgacgtaac
     6661 atttatggat tgtcgtcagc agtatgaaga attatcaaca ggtcatgtga tcaatgttga
     6721 tccagatgtg gtcgcagact ttcgcgatat gccttttgaa gatgatgtgt tcgatatggt
     6781 tgtatttgac ccgccacact taagacatgt cggcgaaaac tcatggcttg ccaaaaaata
     6841 tggaaaatta gacgaacttt ggccagaaga catcaggcaa ggttttgcag agtgtatgcg
     6901 agttttacga ccttcaggaa cattaatttt taagtggaac gaggaacaga taccactttc
     6961 tgatgtgtta gaggctattg gagaacagcc attgtttggt aataaacgaa gcaaaacaca
     7021 ttggcttgtg tttatgaaaa aattcagcta acggaggaaa caaaggagga aaagtaatga
     7081 agattgtatt aaataaatgt tttggtggct ttgggttgag ccatgaagca aaaatggaaa
     7141 tttttaaaag aaaaaatatt gaagtattcc catacatcaa taactttagc tatgactcag
     7201 atgatgaata cacgagatac acaggccaaa agctaggttc gatggatttt atctactatt
     7261 tcaaaaaaga cccgaaaatc gacaaagtta caggaattta tagcgaaatt gaccgactgt
     7321 acggaattgc tgatgattcc agtttttcaa gtgattcaaa cagaggtgat aaggacctcg
     7381 ttgctgttgt cgaaaaacta ggaagtgagg ccagtggacc atatgcaagt cttaaagtcg
     7441 ttgatatacc cgatggggct gagtgggaaa taagtgatta tgacggcgtt gaaactgcac
     7501 attatggttt tcaaacaggt agtatttagt ccactaatga caactaaaga ggaggcagaa
     7561 ggatgagtga agatgaatac tttgaacagt agtctattaa cgacagagat ttttaactgg
     7621 aaaggggtgt agtcatggac gcatcggaag tagtaaataa aattaatagt ctcaaagagg
     7681 ttttcggaga cgtcgaagta gtgataaacg cacaaggcta tgacaacgaa ttttttaagc
     7741 agattaccga cattagcatt cagcaaggaa tggaagacga agacggaaac tttatcgatg
     7801 aagtagccat tttagtgact tgcgagtaag taattgacag ctaaagagga ggaagcggaa
     7861 tgaaactaaa agacggattt tactccagcg gccacggcat cggcggttta atgctagata
     7921 tgccgacaaa gaatcctaaa acacgtaaga aaccaaaatt caaagtcggt gacatggttc
     7981 gctgcgaagc agaaggattc atctatccat ttcgtggata tgtagaacac gtctataatc
     8041 actcagcaat cattcgcatt gaaaacacga tggaatgtga taagtggtta gcgaaaagca
     8101 aagagaattt agcagtggtg agattggtgg acatggaagt tataaaatct taattttgct
     8161 tttttacaac tatcctacaa caatgagcgt tattccacaa ctggaaggag aagaggcaat
     8221 tgtgaacatt ttagagaata tagatattaa gcaaacaagg aaaaatgcta gacgattact
     8281 aaaaagatac agaaatttag aacgtttggt cggaccagtg aaaatagact tttccgtgat
     8341 gactgttacc aaaaatttga aattcacaat tgacagtcaa aacgaagaaa tcattgaagc
     8401 gataagtact cgagattcgg ttatcgaagc attaacacgg ctaagtagaa tccatttcca
     8461 ggtactttat tatagttatt gttttcctaa taagatgtca atgtatcaaa taggagaaaa
     8521 attaggatat tccgatagaa ctatcgagag aatgaaggca gtagctttgg ttgaatttgc
     8581 tgaggcgtat aaatcaggag aactcatttc acgtacaaaa taaaaaaagt cggattcctc
     8641 cgactatgaa taatatttcc gacataagta ttatatcata attgggggaa tcagaggatg
     8701 gtacttttcg atgtaaagaa atatgagaca ccaagcgcga aagatgttga tatggaacgc
     8761 acaaaacata atgtcgctgt gtttctttca gcatatctat cagctagatg tagagtaggg
     8821 caacatcgag aaccaaaagt gacagcatct tattccttgg ttccaccttc tacagctgat
     8881 catatatttg aagcagaaag aatgctgatc gataaagaag aagcacagga agaatttgag
     8941 tatctgcata aattgtttgt tcgaggctat tcagcgatac agcatccgca taagcctgat
     9001 gtgactgaaa ggcgcaagaa gatattctat gatcgttata tcaatggtct gcccatttat
     9061 gtaactgctc aaaggaataa tactagcgaa gaatcggtta aagtagaatc aaacagaatt
     9121 atcatccaat ttgcttcatc gttagaactg gttgctttca agtagccagt ttttacactt
     9181 tttataccct tttattacca atttggtttc cattttatac cttttttata ccaatcactt
     9241 acctattcaa cgttgtatta tgatagtgtc gaaaaattag gaaacaggat cgacaaaata
     9301 aaatgtaagg gaggaaatct ccctcatcgt ttaattaagc ttcgataggc agcaacggaa
     9361 atattaagaa taaggatgtg aatttcaact ccttctaaat tgttcttatt atctatcatc
     9421 cgttgctgtc tattgttttt ttaattattc acacgataac taaaggtaag tgaagagaaa
     9481 tgattccatt aataatttca atttttgcgc tttgtcttaa tgtatatatg attggattta
     9541 aaaatgggca aaataaaaaa tagtagcagc caagaataat tttatagtgt cactgtggcg
     9601 gaaagggtag acgctaagca tgtgtgctag gtcaatgctt cggcaaccat gcaaggttcg
     9661 attccttgcc agtgactttg gcaaccgagg catcggcggt ttaaaaatat aggggtgcgc
     9721 aatttcgtac gcgttttgtg catcgtgtag gttgctatta cataactggt taggttagat
     9781 tgagatttgg gattcggtac aaatgaatcg tcaaatgtct caagcacagg atcggaaacg
     9841 tccctgcctg tgcattacat attagatcac tctttgagtg gtctttttct ttacataaag
     9901 gaggctacat aatgagaaac tactggtatg tatcgctaac taatgaatat cctcgaacca
     9961 ttgatgattg ttcagtgcgt gttgtgcgtt ctgtacaaat caaagggaag tactctattg
    10021 tcgaaatggg gagagaagca gaaccatgtg agattgatgc gtgcaagctc gtttattgcg
    10081 gtcatggttt ctatgatgaa ccaaacattc aaaataatat taacaagaat ttgagggatt
    10141 agaatgcaag aaatggctta aaaatctcca atcaggacaa atcaagttag acaaagtttc
    10201 cgatttaaag atattgattg aagctgattt aatgctaaaa gatattgaaa attagaaaac
    10261 aaaactcaac ctaaacagaa tgtgaggtgg tgtgtattga atggcaagac aacgtgatcc
    10321 aagacgtgat gaagccaaaa gaatttggtt agaatccaac ggagaaaagc agttaaagga
    10381 aattgcatct gaattaaatg tttcagattc tcaggttaga aaatggaaat cgcaagacaa
    10441 atggagcgct gaattgatta gtaacgttac caatggcaaa agtaacgtta ctaatcaagg
    10501 tggcgctcct attggtaatc aaaatgctaa aggtaacaaa ggaaatagcc gagcttctcc
    10561 gccagtgggt aataaaaacg ctttgaaaac aggtgagtat gaaacaatat tttttgacac
    10621 gttaaccgat aatgagaagg acatctattc tagtttgaat gatgatcctt cttttgcttt
    10681 gtccgaagaa atacgacttc ttaaaataag acaacttcga atgatgaaaa gaatccaaca
    10741 agctgaagct ggactaaatg atgaagaagt cgaacgattg cagcagctaa gaaagattaa
    10801 aactccgatt gaaaaaaatg gtaaaaagct agaaatcaag cgtgaggtta tgcaagatgt
    10861 gcagattagc agaaaaaaac atcgcaaaat tgatgatatt ctttcaattg aagattcatt
    10921 gactcggatt agcaaccagt tagctaaagc catcaagcaa atgaatgaac tttatatgaa
    10981 tgaatacaga actgatttaa ttaaagctca gactgataag atccaagctg agacaaatga
    11041 aattggcgga aataattcag gtgaagaaat agaagaatgg aaacaggcag ttttaaatgc
    11101 cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt tattcctttt gctgatattg
    11161 gttctgccat tgattattac tatgataaac cagtagcttt ttgccaagat attttgcatt
    11221 tgaatcctga tgaatggcaa gaaaatgttt taaatgattt agctgaattt tcaaaggttt
    11281 ctgttcgttc tggtcaagga gttggaaaaa cagcattaga agcaggagca atactttggt
    11341 tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc tccgacaatg aagcaacttt
    11401 acgatgtact ttgggcagag gtagctaaat ggttaaatga tagcttgatc aaaaacttac
    11461 tgaagtggac aaagaccaaa atttatatgg ttggtgattc agagcgttgg tttgctacgg
    11521 ctagaacagc gactaaacca gaaagtatgc aaggttttca cgaggaccat atgttgattg
    11581 tggtagatga agcttctggt gtgtctgatc caattatgga agctattctt ggtacgctat
    11641 caggttttga taataagctg ttgatgtgtg gaaaccccaa taatattgaa ggtgtctttt
    11701 atgattccca taattcagac cgtgataaat acagagttca taaagtatca agctatgata
    11761 gtaaacgtac aaacaaagac aatatagaaa tgattcttaa aaaatatgga aaagaaagtg
    11821 atgttgctcg tgtccgtatt tttggagaat ttccgaaagg tgcgttggat tcatttatca
    11881 gtcttgaaac agttgaattg gctacagaaa aacaaattag tgattcttta gtcaataaaa
    11941 caaaggttgc tcatattggt gtcgacgtag ctcgatatgg tgatgattct acgattctct
    12001 ttcctagaat tgctaccagg gcattggagt atgagaagta ttcaaaacgt agcaccatgg
    12061 aaacaacagg atatgtcatc aacatggcca agaatctaat gagtcaatat ccgagtattg
    12121 ataaagtgat gattaaagtc gatgacactg gtgtcggagg tggtgtaacc gaccgcctag
    12181 aagaacttat agaagacaaa cattatcctt ttgaggtgtt tggagtgaat aacggttcaa
    12241 catcagaaga cgatttttac gataatttag gtactcaact atggggaaac atcaaggaaa
    12301 tgttagaaga aaatatgaca gcaaatctta acggagaaca gcctgttatt gaattgcctt
    12361 ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt caaaatgaca agtagaagtc
    12421 gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa tattggtagt cccgatattg
    12481 ctgacgcact ggctttagcg ttttatgagc caccaagtca ctatcaattt attcaatttt
    12541 aggaggtgag cttttgatta gtacagtatt atctttagag agatataaga agctacgtgt
    12601 taaatatgca acacaaattg aagatggaat gtttgatccg aatggtttta tagaagatat
    12661 gaaaccattt tttgctgatc gcgaaagaaa atatctagct tatactagcg aaaagaatga
    12721 aatagataat agaccaaagc ctaacactga tattgtaaaa gttaataata aacttcatgc
    12781 tggtatgtat tcaattgtcg tagaccaagc agtcaatcat tttactggta tacctatcaa
    12841 atgggattac gatgtatcgg aacaaaaaag aacactcatt caaagattaa aagataaatt
    12901 cttaaaaaat gatatagaac ttccgacagt tccagaagct tttaacaaat taacaagcaa
    12961 tcttgattcc atgagattcg caatgcttga ttctgaaact gcaacttttc aaggagcttg
    13021 tggagtagct ttccgattgc tagagcctgt ggaagaagat gatggttgga agttaagagc
    13081 aagtaatatt gaaccttgga gagcagaaag atatggaaat gccggaatct atatcaaaga
    13141 aaagtatgac tcttaccaga aaaaattttt tcaagaaatg aaagttatga caagaaataa
    13201 aattctgaca tatgcttgct atggtgattt gaattttgtt acaagcggaa cgttcaaaaa
    13261 aattgatgag accgataacc ctttggggac gattaccttg gcagaattta aaaataatac
    13321 gaatcgttat tgtgattttg aagtagctga ggaaattgga gatgcaattg atcgtgcttt
    13381 gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg tacatgcttg ttactggcac
    13441 aacaatgagc aaaggcactg ctaaagaaat gatgaatcag ctagggatta tcaacttaaa
    13501 agcccctact gcaaaagctg aatatgtcac aaaaaattta gcaaaagatt tcaatgaata
    13561 tcatatggat ctattgaaaa aacagtttta caccatttgt aaagcaatcg attttaacga
    13621 tgaggtgttt aaatcaaaca gttctggaga agctcgcaag tggcaaatca ttagcctaga
    13681 agcgaaaaca aatactaaag agcaatattt tagagaagga ttgaaagaat gcgcagaaac
    13741 aattgctgct tttcttaagt ttcatgacaa agtagaaatt gagcctgaaa aaattatttt
    13801 tactttctct agatctttgc caactgattt aagttattta gctgaagctt tacctaaact
    13861 tgcaccatac gtatccaaac gaactattca gagccaaatt ccatttgtaa cagatgttga
    13921 ttatgaaaat gaaatgatgg aattggaaag tggaagtgct tatccagata gtgaatacaa
    13981 ttttggcgga ggtggcaata gtgacgatag aaacgaagta ttggaccaaa cgtcgagaac
    14041 tggaggatca agcaaggctc aaacaagaaa atcagacact taaaaaatta actagtgtat
    14101 ttcctgaagc actgaaagag atacaagcaa aactattatc acaagctgac ttacacaata
    14161 tcacttatcc agaaatgatg gagttttaca gtacaagtaa tcagaaaaaa tatcgtgaat
    14221 atgtggaaaa aaattataag tcattaaaaa tgtatgatgc aaaatacaaa gagtttatcg
    14281 atgaattttt tccaccattt gactatgcaa aagtcaatcg cttattacaa atacgatcag
    14341 atgtatttaa aattcttgca gaatatgcga tggatgcaga tgtgaatcaa tatttttctg
    14401 atcgcttaga ggaaattctt caaagaacat attcttctaa tgctaatgtt tttgttcaac
    14461 ttttaaacgt tgatatacca aattatttac cagaaaatga aattaaacat tatttgaact
    14521 atccatggtc aggaaagaca ttttcaagaa gactttgggg aaatatttca tcgcttgaac
    14581 agaaactctc taatgctatt gtaaaaagtg ttgctagtgg agaaggcgtt atacacgcat
    14641 taaacaccat gagattagac tcagaaattt gtgacatgtt taagttagaa gaatcaaagt
    14701 ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc aaaatttgca caagatggta
    14761 ttgaaaaatc atatttagaa acaggtattg aggaatacaa cgtattgact gcaaaagatg
    14821 agagagtttg ccggatttgt ggaggaaagg caagtaagaa tccctataaa ctgaaagatg
    14881 ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg tagatgtacg gatgttccta
    14941 atttaccaaa attaggaaag gatattgatg aagaatatga ccgtttattt ggcgatttat
    15001 tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag tagaatgaaa
    15061 gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga agcctacaaa
    15121 acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa tggcaattat
    15181 gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc agttgataag
    15241 aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt aaaagaaaca
    15301 gttgattggt atgaaaagat gggatgtatt gttgttagaa ctgactacaa agaaggaaag
    15361 tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc aatactgggc
    15421 ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg tgaaaacgaa
    15481 tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat taatgccaat gaatttgcaa
    15541 tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc ttttgtggaa
    15601 tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc cgacaaacaa
    15661 ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat caaggaatat
    15721 gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact acaagctgaa
    15781 tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt tgcagaacaa
    15841 attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt tatgctaaat
    15901 aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt tacaagtgtt
    15961 ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat gaagtctgca
    16021 tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac attaacaaat
    16081 ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga aaaattatga
    16141 aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt aatgctatgc
    16201 aagtaccaaa tacacctttt ttaagctatt tacttggtgc tggaaaaaca gagccagcaa
    16261 actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct tctgaaaaac
    16321 ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac aactatactg
    16381 aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat gtgaatggag
    16441 tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa attgatttga
    16501 accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt cgtcgaatga
    16561 atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact gcgggtgcag
    16621 taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt tatatgggag
    16681 aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag ttagatggaa
    16741 aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa attggaaata
    16801 tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca aaaggaacaa
    16861 ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg agagcagaag
    16921 aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac tctcttgaat
    16981 acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag ggagttagta
    17041 ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa aaaagatgaa
    17101 gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca tcctaaaact
    17161 cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga tgataaagaa
    17221 gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt gatctgatgg
    17281 atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca gatttgagtg
    17341 aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca tacaacactt
    17401 gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg ttgtataaaa
    17461 taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat acaacgatta
    17521 gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt gatttgatac
    17581 gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag atattttaga
    17641 aactacttat gaagatagtt gtattattga aagactgacg gacattgaag attctaatac
    17701 aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt gcgctctttc
    17761 acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa atgtttcaac
    17821 tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag atcgaataac
    17881 aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc ctttttacta
    17941 tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat ctcaaatatg
    18001 aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt gctcaaaaag
    18061 aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct aaaccattaa
    18121 ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct agaaaactat
    18181 caagcggtcg aattgttgga gaagttataa acaatgaaca ttattttaaa tatgtagaag
    18241 aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta actcgtgcaa
    18301 cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca ataaaaatcg
    18361 ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag caaattaaaa
    18421 gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac taaagatttt
    18481 tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga tattcaaaat
    18541 gtttctttct taattgatat tgcattgatt gataataacc ctaatagaaa tttaattaat
    18601 gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga gcaaatatta
    18661 tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg ttttacatta
    18721 gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag aagtaaaacc
    18781 ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac agggaatttt
    18841 ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc aacagctaat
    18901 tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac agcttttggt
    18961 ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc tacaattact
    19021 ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc gcaagatggt
    19081 gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt cttttataaa
    19141 ggacagtttg ctctagattc agatgaaaca tcaacgattg aaggaagtaa agtaagtttt
    19201 actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata tatgtattca
    19261 gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt tacaaaagta
    19321 gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca aaatatcaaa
    19381 caacgattaa attaacgaaa aaagacgaag aaggtaaata tgagcaagta caatttaaat
    19441 ctgctgaatt tttaccagga acagttgtag aagatgctgc aggagttatg gaagaaatgc
    19501 aaacagcgac tgataagcaa tcagttaaaa aagctttaag tcgtgcttat tcatttattg
    19561 cagatacttt atttgaggga caatttactg gtgaagatta ttgcaaagga attgatgctc
    19621 gtgagattgc ctcattgaca ggaaaattat tgaagtctgt tactgcaggt tttgatgaaa
    19681 cttatacaga gacgaaaaaa aagtaagtga ggcgctcaag tcaccttcat ttaagtattc
    19741 gattacttac cgagaattag atataaagac gcaattactc gaagcaggtt ggacgttacc
    19801 agaaattgaa cgtactgact tggatgagtt aatgcgtctt tttgctttta gagatgcggt
    19861 aaaagagcat gaagatgttg agtactatga taatttcact caattttagg aggtgatact
    19921 ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt tactgccggt
    19981 atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag aaaaagtaga
    20041 aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt tctagctagc
    20101 ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat aagtggagcg
    20161 gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat gggatttagt
    20221 aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg tattcaagga
    20281 cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc ttctacaggt
    20341 gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct tggtttcggt
    20401 ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt ctcaaacgga
    20461 aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc tactctttct
    20521 gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt atctcaaggt
    20581 aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa aggtggcgga
    20641 ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa aacctctata
    20701 caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg attaaataaa
    20761 gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt gggcagttca
    20821 atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc atttttaagt
    20881 aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat gccctttctt
    20941 gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat accagcaata
    21001 ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc attaaagatt
    21061 atgggggcaa tagctgctgc taatccattt gttttaatta ttggtgcagt cgttggtgca
    21121 atagctgttt tcggttactt tatggcaacc aatgaagagc ttagaaacaa ggttatatct
    21181 atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa ggactgggga
    21241 attgatactt ggaattctgc taaagaaatg gcatcaaatg cagttgaggg tgtcaaagat
    21301 gcttggtcag gaacaaagga atggttttct aacacctgga aaggtattaa ggatggggcc
    21361 acaggtttat ttgataaaac agtagagact tcaagaaatg cagtcgatag tgtaaaaaat
    21421 gcatggtcta atatgaagaa atggttttct gatacttggc aaagtataaa agattcagcc
    21481 agagaaaaat ggaatggaat caaaggttct attatgtcag ttgctggtcc attgattaca
    21541 ggtataaaga atgcattttt acatgttact ttttatctag agaccttatg gaataattta
    21601 gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc gccggttttg
    21661 tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaataatat gattgctgtt
    21721 tggaataata ttaaagaaag tgccataaat atttgggaat ctataaagaa tatatttgta
    21781 agttatttta caaatattta ttttgctgca ctcaatattt ggacagggtt caagcttaca
    21841 ttgataaata tttggaatga agtggtaaat caagctaaat cgatttggtt aaacataaag
    21901 tattttttta ttaatctttg gattgatata aaatattttg caattcagaa atggattgaa
    21961 ttgaagtttt ctattattca aacttggatc ga
//
LOCUS       DAZKNM010000034        21870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000034 DAZKNM010000000
VERSION     DAZKNM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.034"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG8M9_001025"
     CDS             complement(<1..563)
                     /locus_tag="ACG8M9_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0377815.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG8M9_001026"
     CDS             complement(750..1400)
                     /locus_tag="ACG8M9_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377816.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8M9_001027"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8M9_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0377817.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8M9_001028"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8M9_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0377818.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8M9_001029"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8M9_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0377819.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG8M9_001030"
     CDS             complement(4926..5657)
                     /locus_tag="ACG8M9_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0377820.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG8M9_001031"
     CDS             complement(5661..6926)
                     /locus_tag="ACG8M9_001031"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0377821.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8M9_001032"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8M9_001032"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0377822.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8M9_001033"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8M9_001033"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0377823.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG8M9_001034"
     CDS             12268..13770
                     /locus_tag="ACG8M9_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0377824.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG8M9_001035"
     CDS             13772..14509
                     /locus_tag="ACG8M9_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377825.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG8M9_001036"
     CDS             complement(14680..15873)
                     /locus_tag="ACG8M9_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0377826.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG8M9_001037"
     CDS             complement(15952..16158)
                     /locus_tag="ACG8M9_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0377827.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG8M9_001038"
     CDS             complement(16151..17293)
                     /locus_tag="ACG8M9_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0377828.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG8M9_001039"
     CDS             17805..18440
                     /locus_tag="ACG8M9_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0377829.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG8M9_001040"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG8M9_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG8M9_001041"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG8M9_001041"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8M9_001042"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8M9_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0377830.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="ACG8M9_001043"
     CDS             21086..21253
                     /locus_tag="ACG8M9_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0377831.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="ACG8M9_001044"
     CDS             complement(21313..21666)
                     /locus_tag="ACG8M9_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377832.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         6472 a         4436 c         3880 g         7082 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc
//
LOCUS       DAZKNM010000035        20888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000035 DAZKNM010000000
VERSION     DAZKNM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.035"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..3301
                     /locus_tag="ACG8M9_001045"
     CDS             23..3301
                     /locus_tag="ACG8M9_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0377833.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3842..5749)
                     /locus_tag="ACG8M9_001046"
     CDS             complement(3842..5749)
                     /locus_tag="ACG8M9_001046"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0377834.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5879..6754
                     /locus_tag="ACG8M9_001047"
     CDS             5879..6754
                     /locus_tag="ACG8M9_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0377835.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6834..7808
                     /locus_tag="ACG8M9_001048"
     CDS             6834..7808
                     /locus_tag="ACG8M9_001048"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0377836.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7988..9133
                     /locus_tag="ACG8M9_001049"
     CDS             7988..9133
                     /locus_tag="ACG8M9_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0377837.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9270..9614
                     /locus_tag="ACG8M9_001050"
     CDS             9270..9614
                     /locus_tag="ACG8M9_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0377838.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9885..11171
                     /gene="efmA"
                     /locus_tag="ACG8M9_001051"
     CDS             9885..11171
                     /gene="efmA"
                     /locus_tag="ACG8M9_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0377839.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11309..11908)
                     /locus_tag="ACG8M9_001052"
     CDS             complement(11309..11908)
                     /locus_tag="ACG8M9_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0377840.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12312..13433
                     /gene="mnmA"
                     /locus_tag="ACG8M9_001053"
     CDS             12312..13433
                     /gene="mnmA"
                     /locus_tag="ACG8M9_001053"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0377841.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13679..15100
                     /gene="arcD"
                     /locus_tag="ACG8M9_001054"
     CDS             13679..15100
                     /gene="arcD"
                     /locus_tag="ACG8M9_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0377842.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15114..16232
                     /locus_tag="ACG8M9_001055"
     CDS             15114..16232
                     /locus_tag="ACG8M9_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0377843.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16292..17584)
                     /locus_tag="ACG8M9_001056"
     CDS             complement(16292..17584)
                     /locus_tag="ACG8M9_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0377844.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17734..18294
                     /locus_tag="ACG8M9_001057"
     CDS             17734..18294
                     /locus_tag="ACG8M9_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0377845.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18423..18800
                     /locus_tag="ACG8M9_001058"
     CDS             18423..18800
                     /locus_tag="ACG8M9_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0377846.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18733..20805
                     /locus_tag="ACG8M9_001059"
     CDS             18733..20805
                     /locus_tag="ACG8M9_001059"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0377847.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6766 a         3792 c         4471 g         5859 t
ORIGIN      
        1 atttgttacg ggaggaatac acttgttaaa aaaacgtata aaatacagtg cgctaatgat
       61 gaatctcctt atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga
      121 acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga
      181 ggaaatacca agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa
      241 agaatcagaa aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg
      301 ggttgttttc ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg
      361 cgtgcggctc tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat
      421 gacctattat tatcaagcaa tccccaaaga catcaacttt aggatgcggg cgacgatcga
      481 aatagaatct tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc
      541 agtaccaaaa aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg
      601 cagtcggatc gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag
      661 caaatatacg atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga
      721 tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc
      781 agcagggttc ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa
      841 tctgtttccg gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac
      901 tggtttagag gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat
      961 gtatgactgg gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc
     1021 agcaaggaat atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac
     1081 agatcctcct gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc
     1141 tagtaatcat tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt
     1201 attgacaatt aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat
     1261 cactgcgaat cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac
     1321 tttcagtttt acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta
     1381 tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta
     1441 tgtaacacca gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac
     1501 acaagcattg agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc
     1561 cttcgaaaga ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca
     1621 gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg
     1681 aaatgggatg acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag
     1741 tgcagctatg caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca
     1801 tgcttatcgg aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga
     1861 agcatggccc gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg
     1921 atttgaagat gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga
     1981 cggttgtatc gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac
     2041 aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc
     2101 aggaattgag ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg
     2161 ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa
     2221 tagcggaaca gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt
     2281 ggctctttat acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt
     2341 ccgaacagag aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca
     2401 aatctaccac tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct
     2461 tggacatacc atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca
     2521 gcagcctgtt acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa
     2581 accggggaat cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc
     2641 tatttttgac ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga
     2701 accaaatcag acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg
     2761 tgtggcggag aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc
     2821 aactttcaca ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa
     2881 gatccaaatc gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc
     2941 agcaccttca aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg
     3001 ggaaaaatgg tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga
     3061 aactgccgat gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc
     3121 accaaatgga aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga
     3181 aaaaaatggc actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt
     3241 agctggagga attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata
     3301 agtggaattt ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga
     3361 aatgagacca atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct
     3421 gcttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact
     3481 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa
     3541 ctatttttcc gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga
     3601 taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa
     3661 agccattttc acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta
     3721 atacatggta ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg
     3781 agaagcgatt ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct
     3841 cttaccagat tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac
     3901 caaatgtttc atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag
     3961 gggcatggac atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc
     4021 aaatcgttgc ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg
     4081 cttccaatgc acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc
     4141 cgtttacttt tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt
     4201 tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt
     4261 caaataaagc gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga
     4321 ttccaccata attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg
     4381 ctgccggaaa gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac
     4441 tcatttccca ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg
     4501 tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg
     4561 ttttgaactg gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt
     4621 tatttaattt tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta
     4681 gacgtttttt ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag
     4741 cttcgccaaa atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg
     4801 caaggtaata cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag
     4861 aatagattcc gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg
     4921 ttttaatgat catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca
     4981 aatgatcgaa gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac
     5041 ccgtgacaag ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt
     5101 gcggattata cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa
     5161 tcaatttatc aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc
     5221 cggcaagctc taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt
     5281 tgggatggtc tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa
     5341 agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg
     5401 gtaatgtatc caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg
     5461 attcaacttt ttcgattata ggttgaagcg gttgagcatg caactcattt cttcggtcat
     5521 aatcgtttgc cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt
     5581 ctggatgggc aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta
     5641 aatcttggaa accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt
     5701 cttcattgac tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc
     5761 tccttttttg tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat
     5821 aaaaaattga aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat
     5881 gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt
     5941 agataaaaaa attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt
     6001 gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca
     6061 tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg
     6121 gaagctgatc aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat
     6181 tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct gggtgggatt
     6241 gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg
     6301 tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct
     6361 tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg
     6421 tagccagatc caaccactag gacttcctac gattcctttt caggttagta cagggatttc
     6481 tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg
     6541 gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt
     6601 acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac
     6661 aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc
     6721 cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc
     6781 ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta
     6841 ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg
     6901 aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg
     6961 atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc
     7021 aatcaacgaa tgcacctgtc aatgattact atatggaact actgatcatg atcgatgcaa
     7081 tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc
     7141 aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg
     7201 aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat
     7261 tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc
     7321 aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc
     7381 gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg
     7441 atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg
     7501 tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa
     7561 acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa
     7621 gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag
     7681 aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg
     7741 tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat
     7801 tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc
     7861 tgcgacaaga cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa
     7921 aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt
     7981 ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca
     8041 tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag
     8101 catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc
     8161 agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga
     8221 taatacagct atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac
     8281 tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt
     8341 tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa
     8401 agcgttacga gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa
     8461 tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac
     8521 agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt
     8581 attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg
     8641 agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc
     8701 aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc
     8761 tgaagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac
     8821 attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca
     8881 tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct
     8941 taatggggtc gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca
     9001 cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag
     9061 ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc
     9121 tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc
     9181 attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa
     9241 gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg
     9301 gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg
     9361 gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc
     9421 aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa
     9481 tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga
     9541 aaatgagcca agataaattt tattttctga tggatgggat gattagccgt gggttattcg
     9601 aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta
     9661 gatgactaac tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca
     9721 catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg
     9781 aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg
     9841 ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt
     9901 cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt
     9961 caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg
    10021 gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa
    10081 gcccatttgt aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg
    10141 atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct
    10201 tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa
    10261 tgccaacgat ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga
    10321 atggacagtt aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg
    10381 ctttgttttc agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt
    10441 ttggtgtcgg cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag
    10501 ttccgcttac tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag
    10561 ggttatggta cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca
    10621 gtctgtatcc attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag
    10681 tggaagtagt ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa
    10741 catggaaaga ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg
    10801 gtgcaagcgg attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg
    10861 caggagcagg ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga
    10921 gttatgaatc gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg
    10981 caggtccaat tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa
    11041 tgttcttgtt ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag
    11101 ctgcccgaaa ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa
    11161 aagacgaata gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc
    11221 ctaatgttcc tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt
    11281 gtttaatact tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat
    11341 agatttcttt gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga
    11401 gttctctttc cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat
    11461 gatggatatg tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat
    11521 gagtcaagta atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa
    11581 atttgggact tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc
    11641 gactgtcatg gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca
    11701 aaatttctgc atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa
    11761 catggtcggt ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat
    11821 ccacatttgc aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa
    11881 agtgcatatc actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa
    11941 acaaaatcac ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg
    12001 tgcagcttta agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta
    12061 ttccttattg ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc
    12121 tcaaaagatg agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag
    12181 aacagtattt ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat
    12241 gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg
    12301 atggtgatat catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg
    12361 tcgactcatc tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat
    12421 tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata
    12481 aggatgtagc gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa
    12541 aagaatattg ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac
    12601 ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa
    12661 tggatcttgg cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa
    12721 acggcgtggt ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta
    12781 gccaattatc acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat
    12841 cagaagtacg ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa
    12901 cgggaatctg ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag
    12961 caaaaaaagg aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga
    13021 tgtattatac gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag
    13081 aaccctggtt tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt
    13141 tccatcatcc tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga
    13201 atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg
    13261 ttcctgtgac agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg
    13321 tacgtgcaat cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg
    13381 gtggtttgat cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat
    13441 taaatgaatc aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag
    13501 ctgttttttt gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa
    13561 agttcataat atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa
    13621 aatagaaggg aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat
    13681 ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc
    13741 aatcggaagc ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc
    13801 agcgatcctt tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa
    13861 tcatttagga gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc
    13921 atttggtaaa ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg
    13981 aaatgttgct tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa
    14041 aggtggacaa aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt
    14101 attagtaaac aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa
    14161 attagtgcca ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt
    14221 cactgctgat ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg
    14281 aacgatctgg ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat
    14341 cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc
    14401 gatcattggt ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg
    14461 cgtcttatct caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa
    14521 aggaatcgtg ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat
    14581 tggttcttgg ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga
    14641 cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt
    14701 agttgttact gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc
    14761 ctatcaattt gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg
    14821 gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg
    14881 gattgctgta gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt
    14941 atgtagtatt gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa
    15001 acatcaaatt acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct
    15061 ttcaatcggg ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa
    15121 tcgcaaattt cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata
    15181 ttgcacaaag ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta
    15241 gcgtaagtaa atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag
    15301 gatcagatgt tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca
    15361 tcttacaaac gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag
    15421 caggagacca tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat
    15481 ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt
    15541 tagaacagct agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa
    15601 acaatcctac aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc
    15661 gtacagtaga tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg
    15721 aattcttgtc gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa
    15781 cctactccat tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag
    15841 tttttcgaaa gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg
    15901 ctgtacatgc attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag
    15961 agaaccttgt gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc
    16021 ccaattacgt ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt
    16081 gtatcaattt attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac
    16141 cgaagcatgc ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc
    16201 tcttatctga gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga
    16261 caactgttca agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg
    16321 aagctatgga gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg
    16381 tattgtgtcg tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc
    16441 cgcgataacc aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag
    16501 ttgcttggaa agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt
    16561 ttgtgatcaa tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac
    16621 gtaaggcagc taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg
    16681 caaaacccaa accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa
    16741 atcttggagc ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca
    16801 acgtaacatt cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa
    16861 tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg
    16921 gatatttcaa gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga
    16981 caaatttttc atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg
    17041 taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc
    17101 caagtaatgt tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt
    17161 cttttggcgt cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc
    17221 cttgaagcag acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa
    17281 tgacgattgc cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg
    17341 ctaattttcc ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca
    17401 atccgctcca aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg
    17461 acataaaagg catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga
    17521 caaacaagtt ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag
    17581 ccaattagac tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat
    17641 atgtagttta tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag
    17701 tgaaggattt attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag
    17761 aaaatcatcg actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt
    17821 cagcatttag ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat
    17881 attcgtgatg tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg
    17941 cgacgggctc ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat
    18001 cgatcctttg ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc
    18061 aatgaagctg ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt
    18121 gaggcttttc aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg
    18181 atccacggtt atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat
    18241 ccagaagaca gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc
    18301 agattcattt gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc
    18361 ttgcaacttt cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga
    18421 gtatgtatca agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact
    18481 gggaagaaga tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt
    18541 acgaaaaaca atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact
    18601 ttctcagcgc cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt
    18661 tacagcaata caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca
    18721 gaaaggaatt ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta
    18781 agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc
    18841 gttacttggg cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa
    18901 tggcagagct ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca
    18961 cttcctaaaa caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc
    19021 tcagaagcag tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc
    19081 ttagagtatg tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat
    19141 aaagcaatca aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat
    19201 gaatctgctc ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc
    19261 ttaactgtga agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca
    19321 ttagtgagag accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc
    19381 acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa
    19441 acacaagaag aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca
    19501 gaagtccaag aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc
    19561 caaagagaag ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa
    19621 agtttgtatg agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca
    19681 aatgacatga aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag
    19741 gtcaagcagt atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt
    19801 aaagtgaccg atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta
    19861 agtaatgaag agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct
    19921 caaccccata aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt
    19981 tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa
    20041 acagtcaaat gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta
    20101 tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt
    20161 ctgggaaccc cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg
    20221 gaacgtaccc ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta
    20281 aatccctctt tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt
    20341 gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc
    20401 ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag
    20461 aaatgcccag aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat
    20521 gtctgcacca attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat
    20581 cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc
    20641 cgttgtaaat acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa
    20701 atgaccaaac atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa
    20761 gaaagcccat tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag
    20821 gttgtgatag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca
    20881 aatttttg
//
LOCUS       DAZKNM010000036        20425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000036 DAZKNM010000000
VERSION     DAZKNM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.036"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            631..2331
                     /locus_tag="ACG8M9_001060"
     CDS             631..2331
                     /locus_tag="ACG8M9_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377848.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(2433..3266)
                     /locus_tag="ACG8M9_001061"
     CDS             complement(2433..3266)
                     /locus_tag="ACG8M9_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0377849.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3570..5735)
                     /locus_tag="ACG8M9_001062"
     CDS             complement(3570..5735)
                     /locus_tag="ACG8M9_001062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0377850.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            5995..8289
                     /locus_tag="ACG8M9_001063"
     CDS             5995..8289
                     /locus_tag="ACG8M9_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0377851.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            8282..8947
                     /gene="pgmB"
                     /locus_tag="ACG8M9_001064"
     CDS             8282..8947
                     /gene="pgmB"
                     /locus_tag="ACG8M9_001064"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0377852.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            8940..9947
                     /locus_tag="ACG8M9_001065"
     CDS             8940..9947
                     /locus_tag="ACG8M9_001065"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0377853.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            9969..10988
                     /locus_tag="ACG8M9_001066"
     CDS             9969..10988
                     /locus_tag="ACG8M9_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0377854.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(11269..12636)
                     /gene="nhaC"
                     /locus_tag="ACG8M9_001067"
     CDS             complement(11269..12636)
                     /gene="nhaC"
                     /locus_tag="ACG8M9_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0377855.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(12745..14166)
                     /gene="tyrP"
                     /locus_tag="ACG8M9_001068"
     CDS             complement(12745..14166)
                     /gene="tyrP"
                     /locus_tag="ACG8M9_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0377856.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(14409..16286)
                     /gene="tdc"
                     /locus_tag="ACG8M9_001069"
     CDS             complement(14409..16286)
                     /gene="tdc"
                     /locus_tag="ACG8M9_001069"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0377857.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(16577..17833)
                     /gene="tyrS"
                     /locus_tag="ACG8M9_001070"
     CDS             complement(16577..17833)
                     /gene="tyrS"
                     /locus_tag="ACG8M9_001070"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0377858.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(17945..18150)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18413..19714
                     /locus_tag="ACG8M9_001071"
     CDS             18413..19714
                     /locus_tag="ACG8M9_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0377859.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(19783..20382)
                     /locus_tag="ACG8M9_001072"
     CDS             complement(19783..20382)
                     /locus_tag="ACG8M9_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0377860.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         6198 a         4276 c         3675 g         6276 t
ORIGIN      
        1 taaaactatt taaagaatcc acataaaatg gatataaagc gatcagtaac cctacgacga
       61 tacatattaa taaaaacaaa cgaaacaaaa gtcgacgaga ctgtttttgc ttactctgct
      121 tcacatgtgt cctcccttgc aagttttttt gttctttctt ttattatatg ggttcaggaa
      181 aaaaatagaa aatgaaagcc gatgaggttg tgaaaaagct gtcctgcatc aagtaataag
      241 tacagcccaa aaaaatagct actcatattt tttgttgggt tgggcttatt accgcagatg
      301 caagcttttg aacaccgttt aaggaagagg ttgtgaaaac tgcgttatgc tttgagtaat
      361 aagagcgcta gtcagaaaat agcttctcat attttctagg ctagtgagct tattaccgag
      421 aagcagctga ttgaacaccg tttaccgaga ggtcatgaaa aagctacttt gtttcgagta
      481 ttaagaaaaa ttctgtttca aaacaaggag atgcaacaaa acttatgtcg catcctctct
      541 ccttagaact ttcacttatg tttttcttgt aaaattcata aatattttca tgaattttct
      601 aatttcttgc ctacctctcg cataggatat atgatgaatt tatctaaagt ttgcaaagga
      661 gttcgacaat tgaaaaaata tggcccattt ttagcaattt ttttcattgc tttggcattt
      721 ctcatgcctc atttattcac acataaacta attttgggtg ctgatgcccc attccattac
      781 aatcggttct atgatgccgc gcaacaaatt aaatatgggg atttccatta ttttccttcc
      841 ctttatggat tccagcaaag tggcaggatc gtgaatgccg tttacggccc atttttcgct
      901 tatttacaag gattgatcgt gttgcttgca ggtagctggt ttcattatca gattctttca
      961 aatttcattc tttatcttat ttcaggattt tctatgctga ttttactgag atatgtcaat
     1021 gttcgaaatt gggtaagtgt accagtagcc gttttattca tgagtacgta ttctattcaa
     1081 tactggtcta tcaaccaagg attcactagc tgggggactt gtttttatcc tttatgtatg
     1141 atccctattg ctgatttcgt ccttcataaa aagttccctg ttatcaaagt cgctgtctca
     1201 gtgggactta tgacacagat ccatttactg tcttcgatca tgttagcttt gatgtatatc
     1261 cctttttatc tttattactt ttttaaccag actgaaaaag gaaaagcact gcttcaactg
     1321 attaagtcgg tcttgcttta ttttgttctg acagcaaata tttgggcagg attagctatt
     1381 gtttacagtg ggaataagat tcttcctccc tttgttaaca gagaaatgag tcaaaaggcg
     1441 atagatctgg atggcagtta ttggctccat taccctaagg cctttcctat attgatatta
     1501 tcaggttttt tgctttttat tttctttcaa caaaagaaca ccttattcca aaaaatcgtg
     1561 ttgtacacaa gtcttgtttt cttttttctt tctactagct tgattccatg gacgacattg
     1621 atcgaaaaaa atgtgccttt tattagtttg atccagtttc catttcgatt tttcgtgcca
     1681 tttacgctat tgcttctact ttaccttgcc ctttcactga accagtggtc tgaacaaaag
     1741 tggttccgtc ttttaagtat atttggcatt cttatttttt cagcacagac gatccagaac
     1801 ttataccagc atctggaaaa gtgggaaaat gaaacatttg tcagtcgaca tacttatcta
     1861 ttcgatacgc cggaagaagc aaggaaaaca ttcttcagca aagatatgtc taccagcttg
     1921 ttcgttttcc aaaaaaccac acctgactat ctgccaatct ataaggagac aaaagacaat
     1981 aaatacgacc ggtataacga agaaatacta gaaaacgaaa caacttttat aaaaacacat
     2041 ccaggcggtc ttttgacaat caagtggaaa aacactgggg acaaaactgt tcatatccct
     2101 gttatcgtct acgatcggac tgtgctacag caaaatggaa aaacattgac tgattatgaa
     2161 gtaacagata tcgggacccc aatcgtgaag cagcaaaaag gaatcaacga gctcacgctt
     2221 cattatcaaa cgccaatcta tttctatttc attctttctc ttactttgat tggctggttt
     2281 accttgctat gtttatttat ttatcataga tacaagcttt tacgtgctta ggcaaaaggg
     2341 ataaatacat ccgatcaaaa aactctccaa acggtataat cgtttggaga gttttatttt
     2401 aatcagtttt tttatgttat atgttatatt ttttattcgt tcacatctaa ttcgatttcc
     2461 accccgtagt gatcactgat gattggacta tcgatcccat caaaaatccg ttgatatttt
     2521 ttaacctgca ttccttcagg gacgaaaata aaatctatac gcaatttttc tgtattttgt
     2581 tcccaaccat ctattttctt ttccacagta gctgcttttc ctttttcatt cgcgacaaca
     2641 tacgaatctt gaagaagaag gcaactttct cttacagtat aataaccttg agagtctact
     2701 ggattattga aatcacctag gaaaaaaagt ggtacgcgag tttccaaaaa gtagttttcc
     2761 aagtttttcc actcaaaata aaagccactt tttttatctg tccaccaaga aaagtggcag
     2821 ctcgagacaa aaatgtcctg atcatctatc tttgttttcc caatcaagat cttgcgtgtc
     2881 tgataatctt ctttcttttg aaattgggag acggtaagga cctgactttc caatgttttt
     2941 ttcgtaagca gtgcatttcc ttcttcatat ttatcataac cgatgtggct catttcccaa
     3001 ctccaaaaat actcttttcc gtgttccttt aaaagctgaa ctaaacggta ggcaaaatta
     3061 tctttacgga taggtacttg attctttacc ggacagaact tcattagctt cgcaggttct
     3121 acttcttcag attccagtaa ttggttgatt tcttgtaaag caatcacttc ataatcttct
     3181 aacaggatct tatctgctaa ttcttgcaat tttttctctg gttctggctc aagccagcta
     3241 tgcgtgttga gtgtcatgaa tttcattggt tttctccttg tcttcatttc gaaacaaaag
     3301 ggttgtgaca aaatttttgt cacaacctct ccagcaaata gtgttctcct gctaactcct
     3361 cagtaataag ctcaatggac caggagctat tttttgtttg gctgttttta tcacttggtg
     3421 cagggcagct ttttcacgac ctctccacta aacagtattc aaactgctgc ttctcggtaa
     3481 taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttatgacttg
     3541 atgcaggaca gctttttcac aacctctcct tacattttaa tggagccgat cactttacca
     3601 gctgtcactg tacctaattg cttgatcact acttcttcta ctttatcgtt tgtaaatacg
     3661 atcatgatcg ttggatcttt ttcttcgttt tggatcacat caagatccat ctcagctaat
     3721 ttcgttcctg ttcctacttc ttggccttct ttgacagcaa tctcaaaagc tggctgattc
     3781 atttggactg tatcgattcc catatggatc aaaacttcga taccatcttt cgtttccagc
     3841 ccaattgcat gtttgctagg gaaaatactt gtcacttttc cagcgatcgg cgcaacaact
     3901 tctcttgatg ttggaataac agcaaagccg tcacccatca ttttttgtga gaataccgga
     3961 tcttttactt cagataaagg aatcacttct ccagaagctg cggaaactag ttcttgttct
     4021 atacctaagt attgcttact acctgcttgt ccatttcctg tagaaaccat ttctggctct
     4081 ggaatctctg ctcctgattg cagcagatct tcgatatctg atttcaagac atctgctttt
     4141 ggtccataaa ctgcttggac tccattgtct ttgacgatca atcccatcgc ccctgctcgt
     4201 ttccaggctt cttcagtccc tactttttct cgatcattta cacttacgcg taaccgagtc
     4261 atacaagcat cgacatcttt gatattttct tttcctccca gcaaatgaat gatctggacc
     4321 acttgctgat ctgctgttcc tgttccgctg ttagatacaa cggtttcttc actgtcattg
     4381 tcgtaatttc cgttacgtcc cggtgtagca aagttgaatt ttttgatcaa gaaatttgct
     4441 aagaaatacg taacaacacc aaaagcgatc acacacaaca cgaagttgat aagatctcca
     4501 cctagacccg ctttgatagc taacggtgtc cgagtcagca attcgatatt cccaaatgaa
     4561 tggacccgca atggcaagat atctgccatc gcaaatgcag ccccttggat cactgaataa
     4621 atgacatata atggcacagc agcaaacata aacatgaact ccaatggttc tgttacacct
     4681 gttagaaaga cagccaacgc tgctgaaaaa tacattgatt tatatcttgc tttcttatca
     4741 ggatcgacat tacgatacat cgcaaaagcc attcccatca agatacctga agaaccaatc
     4801 atttgtccta ctttgaaacg agccggcgtc cagttatgca acacaaactc atattgcgac
     4861 atatcccctg cacctttaag attcactaaa tcagtggccc aagccaacca taacggatct
     4921 tgcccaaata cttgtgtccc cgcctgtgcg cctgataaaa tctcatacga tccgccaagc
     4981 tgtgtatagt tgataggaat cgtcagcata tgatgcaatc caaatggcaa tagtaaacgt
     5041 tctaatgttc caaataaaaa cggtgctaga atcggtgcgc tttcttgtga ttctgcaatc
     5101 cataaaccaa aattattgat tccagcctgt acgtttggcc acaccacagc aagaatcaat
     5161 gccacgattg ttgaccataa gataacaaca aacggaacaa agcgctttcc attgaaaaat
     5221 gacaaagcat ccggcaattt tctataatta tagtacttat tataagccat tgccccgaca
     5281 aatccagcga tgatccctac gaatactccc atattcaaag ctggtgcttc caatacactt
     5341 gtaaaaaatc ctttaaccat gatcttcgta ccgaacagag tgtgggtaaa tgcatcttca
     5401 ttcgcaagca tgtcacttgt gacaccaaag atagccccag tgatccgatt gattaaaata
     5461 aatgagatcc cagctgcaaa tgctccgcct gctctttctt ttgcccaact tcctcctatt
     5521 gctaaagcaa ataaaatatg aagattacca ataattgccc aaccaatatt ttcaattact
     5581 ccgcccgttg tgacaagcgg cgtccaatca ggattgatca agggaattga tttccctata
     5641 ctgatcatca acccagctgc cggcataact gctaccacaa ccattaaagc ctttccaaat
     5701 ttttgccaaa actcaaaact aaatatcttt ttcattccgt ttagctcctt tacgcaatcg
     5761 gtttcgttaa acgtattata gcgtgcgttt tcttttttgt aaatgcttta ttttttattt
     5821 ttctgtctat accattctat aaaacctata atacgtgtgt tcccttctat aattttctcc
     5881 taaaaattta tttttttaag cttttttcta tgaaatattt tcttttgtct attgacttgt
     5941 gcaatcggtt gcgtttatta tgaacgtata aaacagctca ttaggaggag aaaaatgaaa
     6001 cagatcaaac gactatttgc tattgatcca tggaaaatca gcacgacaca attagataaa
     6061 aaaaatcttc gactgcaaga atctttaaca agtattggaa acggttatat ggggatgcgc
     6121 gggaactttg aagaaactta ctcaggggac caccatcaag gtacttattt ggcgggtgtc
     6181 tggtatcccg ataagacacg tgtcggctgg tggaaaaatg gttatccaga atactttgga
     6241 aaagtgatca acgccttgaa ctttatcgcg atagatattt cgatcaacga tcagctgatc
     6301 gacttggctt ctttggaacc agaagacttt tactgggaat tagatatgaa aaatggtgta
     6361 ttatctcgta cattcacaat cactacttct actaataaag tacgcatttc cttcgaacgt
     6421 tttctgagca ttgtcaaaaa agaatcagct tatatctatt taactataga aatgcttgaa
     6481 ggtagcggaa cagtcaaact cgtatcaaaa ttggatggaa atgttcaaaa cgaagacagc
     6541 aactatgatg aacatttttg gaatgagatt gatcgtggaa ctatagaaga aatcggctat
     6601 ttgacgacta aaactattcc aaacaacttt gggattgaac aatttactgt tactgcagca
     6661 atgcgccatt tcgttaatgg aaaatcagtc atccctgact atcacgaaga tactttagca
     6721 ttacatggtt gcttgtctgc agaattatct caaggtgaga catggtcagt ggaaaaagaa
     6781 attatcgttc ttactagtag agatattcca gaagcgcagc aagcagaaaa agctgtctca
     6841 gaattaacga accagacaaa caattatatc caagcaaaag aagagcaaac agcggcttgg
     6901 gcagaacgat ggaagcttgc cgatgtggtc atcgaaggag acgaagaagc acaacaaggt
     6961 attcgattca atttgttcca actattctct acttactacg gcgaagatga acgattgaat
     7021 atcggcccca aaggattcac aggagaaaaa tacggcggag ccacttactg ggatacagaa
     7081 gcgtatgctg tccctcttta tcttgcttta gccaaacctg aagtaactaa gaatctgctg
     7141 aaatatcggc ataatcaact gccgcaagca atccacaacg ctcagcaaca aggattaaaa
     7201 ggcgcgcttt atcctatggt caccttcact ggtgtcgaat gccataacga gtgggaaatc
     7261 acattcgagg agattcaccg aaatggcgcg attgcctatg caatctacaa ttatacgaat
     7321 tacaccggcg atacggaata tttgaaaaat gaaggactgg aagttttagc agaaatcgca
     7381 cgcttctggg cagaccgtgt ccatttttca aaacgagcaa ataaatacat gatccacggt
     7441 gtgaccggcc caaacgaata tgagaacaat atcaataaca attggtatac aaatacgatc
     7501 gcagcttggg tcttgcgtta cacgcgagaa aattatttaa cttatcaaaa agaagctgct
     7561 atcactatca gcgaagaaga actagccaaa tggaaagata tcgcagagaa catgtacttc
     7621 ccagttgacg aagaattagg aatctttgtc cagcatgaca cattcttaga taaagatctc
     7681 atgcctgtat ccgatattcc cacaagcgat cttcctctga atcagcattg gtcttgggac
     7741 aaaatcctta gatcttgctt catcaagcag gcagatgtac tacaggggat ttatttcttc
     7801 aatgatactt ttacaaaaga agaaaagcaa aaaaactttg atttctatga accaatgact
     7861 gtccatgagt cttccctttc gccaagtatc cattcgattt tagcagcaga attaggaatg
     7921 gaaagcaaag ctgtagaaat gtaccagcga acagctcgct tggatttgga taattacaat
     7981 aacgacacag aagacggctt gcacatcact tccatgactg gtagctggtt gtcgattgtc
     8041 caaggatttg ctcaaatgaa aactgcccat gatcagttga gattcgcacc atttctgcca
     8101 aaaacttgga aagcttattc tttccatatc aattatcgcg gacgtttatt gttcatctca
     8161 gtcacagatg aacaagttac tttcgagtta ttagaaggtt ctcctctaga aatttacgtc
     8221 tatgaacagt cttattcttt agcggatcgt ctagaaataa cgctaagaaa ggaggaagca
     8281 aatgtttaaa ggggtattgt ttgatttaga cggtgtcatt accgatactg ctgaattcca
     8341 ttatcgtgct tggcgaaagc ttggtcagga aatcggcatt acgatcgacc acacattcaa
     8401 cgaacagcta aaaggcgtca gccgagaaga ctcactctct ttgcttttag cttatggcgg
     8461 aaaagaacac tctttttcaa aggaagaatt tgcagaatta gcgaaaagaa aaaacgatta
     8521 ttatttagag atgatccaga cgatcgaacc aaaagatgtc ttccccgggg tcgtgccttt
     8581 actggattct ttaaaagaag aaaaaatcaa gattgctctt gcttctgcaa gtaaaaacgg
     8641 gccattccta cttgaaaaaa tgggtcttac accttacttt gatgctattg ccgacccggc
     8701 agaagcaaca aacggaaaac ctgcgccaga tatcttcctc cttgcagcaa aagctgtcgg
     8761 actaaaagca gaagaatgta tcgggatcga agacgcgcaa gccggtatcc aagccatcct
     8821 ctcaagtggc gctcaaccaa ttggcgtggg acgtgctgaa gaccttggag aggagatccc
     8881 gcttgtccct gatacgactt ttcttacgat cgattacttg aaagaaaagt ggcatgacca
     8941 tggataatcc agagatctcc gtcgtaccag ttccagagac tgaaataaag aaaatcacat
     9001 tgaagaacga ctacttagaa gtcgttcttc tcaattacgg tgcccgcctg caccaaatct
     9061 tcgcaccaga caaagaagga aaatcagaaa atgttttatt gtcttacgat cgtttcgaag
     9121 atgttctaac agacaaaagc ttttttggag caactgtcgg acctgtagcc ggaaggatcc
     9181 gggatgcttc ttggggtgag catagattgg agaaaaactg cggatcacac catatccacg
     9241 gtggcacgaa aggctggtct ttccaatttt gggaagtaga agttttcaaa actccatcat
     9301 caataggtgt ggtcttttac ttaaaagatg aattttctac ctatccagga ccaatcactg
     9361 ctacgatcac ttaccgttta acaaaaaacg aattagagat gacaacctct gctagtagcc
     9421 ttgttgaaac catctgcaat ccgaccaacc atgcctactt caacctttca ggaaatggaa
     9481 caagagacat ctatgaacat cagttaacgg tctttttaga tggtgtgctt gaattagatc
     9541 aagaaaaact gcctactgga aattggacaa aaaaagaaaa actgccaatc gactttagaa
     9601 aatccccaac actgcaagag atccttgcat gttatccaga tggactagat gatgtatttc
     9661 ttttgcatca tcctaggctt tccaaaactt ccttacaatt attcgagaag cattctggtc
     9721 gccaaatgac tatagcaact tctaataaaa gtatggtatt attttctacg actggattcg
     9781 aagctgattt ctctgtcaat ggaaaacaga tgcattcaaa ctacggatta gcaatcgaac
     9841 cgcaagaaat ccctgatatc gttcatttcc ccaaatttgg atcgatcaac ctccatcctg
     9901 gtcaagaacg gatcagtcag acgatttatc ggttctctgc acaataacaa gagaaaggtg
     9961 tttttatcat ggctattacc gtaaaagatg tggcgaaaaa agccggtgtt gccacctcga
    10021 ccgtctctcg tgtcatcaac gatcatccta gtatttccga gacaacaaag aagaaagtcc
    10081 gaaaaattat ggatgaacta ggttatgttc ctaacatgac tgcccgcaat ctaggaaaac
    10141 gcatttccag tgccatcggc gtaatcttac cccctcttga ttcaaaagag cggatcggta
    10201 acccatttta tcttgagatc atggaagcaa tcaacgaaga agcgcggaat tatgatatga
    10261 ctacagcaat cgctactgct aaaaattttg aattgctgtt agagaacgtc cagcgtatgc
    10321 atttacaaaa acaagtagat ggttttattc tagtgtattc agatagtcaa gatactgtca
    10381 ttgattattt ataccaaaac cacatccctt tcactttgat cggacagcct tatcatcatg
    10441 agaacgaaat cgtttatgta gataatgata accagcttct aggtaaacaa gcaacagaat
    10501 ttttgatcca gaatggacat aagaatatcc tgtttgtgac aaacacgact catgaaagtt
    10561 tgtatttcga acgatatttc ggttatcaaa aagcaatgat gatggctggt cttcctgcgt
    10621 tatccagtgt agtactagag catccggagg attatctgtc ctttgagaaa acgttgaaag
    10681 aaaacgaagc cacagcattg gtggtcattg acgatatctt cgcccttcgc acgatgcagt
    10741 tggcgcaaat gtatggctat gtcattccag agacactctc cgtcattagc ttcaacaact
    10801 cgatcttctc aacattaaca cacccttacc ttacaagtat cgatatcgat atctccgaat
    10861 taggacgtgt cgctatgcaa aagctgcgcg aattgatcca tgaagaattg agcagtggcg
    10921 tacgtttggt catccctcat cgcttgatca aaagagagac agtcgttcct ttagaaaacc
    10981 catactaaat agctgactca ctaaatacgc tgaccattta tcctatggtc agctttttat
    11041 gtaaaagaag atactacttc taacacattg tttatctgta aaaaagagcg tgggacaaaa
    11101 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact
    11161 ccttcggaaa taagctgaaa ttcacaaaaa tttaaaaagc aattttcttg agtttcttct
    11221 tatttcttgg agttaaacgc ttctgtccca gcctcttttt tccttccttc aattttttac
    11281 acgtcgacga ttcaataaaa aaccgcctaa tattgtaaag acaggtgcaa gaatcgagaa
    11341 aaaggcgaat ggcaagtaat gcaacgcgct aactttcatc gttcccataa tgaatgtgcc
    11401 gctgactccc cacggtacga gagaattgac ggtcgcacct gaatccgcta gtgttcgtgt
    11461 caaataggtt tttgataagc caacgtcatc aaaagaacgc ttgaatgttt ctcctggaag
    11521 aatgatggac agatactgtt ctccaacgat cagattgatt ccgatacagc tcaaggcagt
    11581 aaacccgact aaacgagaag gtcggtccat cttttctttt aattcttgaa cgatggtatc
    11641 aacgatcaga tatttgatca gtaacccacc taatgccaat gcaagtaaaa tcaaactagc
    11701 agaacctaac atactcatga tcccgcctcg agaaagcagt tcatctagct ttggatcagg
    11761 tgtatttgcg acataaccat tcatcaagat gtctgctgct tttccgaccg tcaaactttt
    11821 ctcatggata aaagaaagaa cgagagcagc tgtcgatccc gctagtaatg tagggatagc
    11881 cggaattttt ttgattgccc ccccaaataa aataactagt ggaatcaatg tccaaggaga
    11941 gatccaaaag ctcgctttca tgttttggat catttcattg atactagcta gatcggctga
    12001 atgactgtta tgtcctaata tcgtataaaa aatcaacgaa aggacgaatg ctgggaaatc
    12061 cgtataacgc ataccagcga tatgttcgaa tagattaact cctccaatac ccgctgccaa
    12121 gttggtcgta tctgataaag gcgagcagtt attccctaaa aaggcaccgg aaacgactgc
    12181 gcctgtagtc attgcatcat tgaatccaag taaatgaccg atccccaaaa aagcaattcc
    12241 aatcgtcgaa atcgttgtaa atgaactgcc gacagtcact ccgacaattg cacaaacaag
    12301 aaatgctgtc ggtaaaaaga aacgcacaga aacaagttta aatccataaa ccataatcgt
    12361 agggatggtt ccagataaaa tccatgaagc aactaagaca ccaatcaaaa gaaaaatcag
    12421 gattggaatg atccctggag tgattccttt gatgatcccg tcatggatct catcccatgt
    12481 gaacttttta aatctgccat aaaacaacaa acacatgaaa gctgcaagga tcgggatatg
    12541 tggcgagagc tggaatccga tgatcagtac tcctaggagt gccaacaaca cgatcaatac
    12601 agcgatactt tcttttaaag aaatattttt attcatcgtc gatctcctat ttcaaatgaa
    12661 aaggccctgc catgaaaaaa ctattcactt ggcaggacct cctactaaat cagcgacaaa
    12721 acgtcggctg aaagacttat ttttttattt ttcttctaaa cttttttcta cctcaatccc
    12781 ccaagaatct ttcttgaagt gattgatgat caatggaata acgaacatga ccactgtgat
    12841 agctgctact aataatgcat cactcaatgt cgctgtacct aatgtagcaa atacgacaac
    12901 gatgatagag aagatcagca tgcagctgac tacccaagct aatccgtttc cgtttttacc
    12961 aattcggtaa ggacgttcga attctggatg tttcttacgc attttaagca atgaaacagc
    13021 aatcaaaatg tacacgatac aataaataac agttgtagca tttgtaagtg tcaagaatac
    13081 agcacttacg tcatccatta caccataaag caaagcaaat aatgaaataa cgattgattg
    13141 tgttagaacg acatttctag atacgccgta tttgttttct ttatggaatc cgaattttgg
    13201 tggaaggaat ccttcacgag ctacttggat gattgttttt gatggtccag taacccaagc
    13261 tgataattgt aaaagcacac caataaatac catgaagctg aagatatttc cgatcacttc
    13321 tggtaagcct aggatttgac agtcaatcaa gataggctgc gtgatatttg ctaattccat
    13381 ttttccatct ggtacgacat ttgctacaag catcgcattg atgacattca ataaaactaa
    13441 tccgatcaaa gcgataaata cgccttttgt ataatttttt gttgcgtctt tcaaacgagg
    13501 catgtaaaca gaactcattt cgatcccaac aaaaataaat gtgataccag ctaagtattt
    13561 caatgaatca atatgttcca agtcagggaa agcttttgac catgaaaagt cacctagata
    13621 gccgtttgag actagtccga ctttgaaggc tgccaaaaca cctaaaacga acatgatgac
    13681 tactgggata tataccccta gccatacacc gatgttcccg ccgacttttg ccatatcgaa
    13741 tttcaagttc aagatcgtga tgatccagta gataactaaa atacaaccta aaacgaacca
    13801 gtgattattg ccaagttcta cattaccaaa tgtgtttcct aacaatggtc ctaaagtaga
    13861 agcgaccatt accatacctg ggaacatttg cacccataat agccatgcaa cgacaaatcc
    13921 ccatttgctg cccagtgctg tttttaccca taactgagga ccgccttctt gaggaagcat
    13981 cgtggaaagc tcaccggaaa ccattgatat cggaccggca aaaaacaaaa ctgcaaaaat
    14041 tgaataaaaa atcaatgtcc aaccgactgc ggctaacgta ggaatactcc gaaccgttgc
    14101 acacagtgcc attgtcagac cgataaaggt cccaagaccc attttactta cttcagattt
    14161 actcaccgtt gacactctcc ttgatttttt ggataaaata acggtgtctt tgaagtagtt
    14221 tcagcaataa acgaaacaat tcagcgaata tttgagggga ttctttgagt ttattgcttg
    14281 aagggtttct tcaaagacaa cctcaaaaca aagggatcga agagcttagg tctccccttg
    14341 ctcttcagat ccaaacatgc gtcagaaaca gttagtattc aagctgtttt ctaacatgat
    14401 taccttgctt attttgcttc gcttgccaat aattggtctg cgtagatttt ttccaatttt
    14461 tcttgaagag ctgctttgat tttttcagca tattcttcaa agtgttcttc tttgttcatg
    14521 tatggtgtca ttacgctggc acgcaataca gttacttttc cggcacggtt ccattcttca
    14581 tctgagaatc ctagttggtt cacaaattgt aatgggctgt tgccatagtc tgggatagca
    14641 aagtcagtat gtgaagttaa gaactcatta ccgtaaatgc ttcctttcac gtatgaagaa
    14701 taatcatata cgtcatggtt caatttattc attgcaacta agtcatcgtt gcctttttct
    14761 ttgaatacat agtctaccat attgaaatct ggataagtta atggatgaac ttcgatttct
    14821 ttgtctccta ctttaaatga caagtcattc aagaagttgt agaaacgatg tgcaccttcg
    14881 attgatgcac ccattaattt accatatcct gtgacattca aaggtaagac atggtgtgct
    14941 gcccaaacgc tggctgctgt tgcccctgct tttgaacctt ccaagatata agccccaagt
    15001 aatgctggga tatctgcgcc tttttcaaat acatatgtcg caaagtaaga gataacgtct
    15061 ctcatgcgga tatctttgat gacgatacca ccggcagaat atggaacata gcccatttta
    15121 tgtgggtcaa tcgttacaga ttctgcttct tcgattgctt tatatgcact gtgtacttct
    15181 tctaagatat aatctttgtt ttctgtaaag acattgtatt tgtaatgaac atcttttaaa
    15241 tcttcaaatg gaatgaagtt gttgtcttcg tctaagaaaa tcgcacggcc gtatccgcca
    15301 taagcagcat ctacgtgtaa gtagaagtaa ataccgtctt tttctaatac gcgacgcaat
    15361 gcaacgattt tatcgatacc gtcgattgct ccttcttctg ttgatcctac aacaccgact
    15421 acacctagga ttggtgtttt ttcagctgct agaccacgaa cgattttttc aagttcgttg
    15481 atgtccatgc gatagttgtg gtcaactggc actggaatca cttggtctaa accgataccg
    15541 atgatgtcgg cagcttttag ccatgaatag tgtttagttt gtggaaccaa ccattttcct
    15601 aatttttcta aatgttttcc gctacgtgct gagtgagctt tgatttcgtc gattttttct
    15661 gggacgctgt ctaacagatt catgatttct tctgttgaca agttcattaa ttcccaatcg
    15721 ctttttccag caactagttc aggtgtcact tctttcattg caagcggcaa tgatttgatg
    15781 ttgcgggcat accaaagtcc ttctaagttt gctaaagaac catcagcaac gatgtggccc
    15841 cagccatctt tatagctcat taattttgca aattccattc ctacttcttc ttccatttgg
    15901 cttgttgcag gagaagattc ataggcaacg ttattaccat tccatagcat agcaaaattg
    15961 tacgctagta aagatggcat caatgtttca gagttcatat gaccccagta acgtccggca
    16021 ttatgccaag gaacggaatg ggtacgcatc cgcgctgaaa tttctgatag aacatccttt
    16081 gttttattca cagtgtgttc aaaactagca ctgctttttt cttctggcgt aataataggc
    16141 atgtcttgag gcatgtagtt ttgacgccag cctaaatgtt catccaccaa ttcattcaac
    16201 aaagctttat agatttggcc gttctcagct ttgtccccaa tgaatagggc atttaggttt
    16261 agatctttcg acaatgattc actcatagtg aaaccctcct aaaaaaatat ttaaattttg
    16321 tcgagcccta cattacacct gttcttctat tctgtaaact aagttttggc ctgattttta
    16381 atcatatttt aatatgtatc aattatccga attgttcaca cttttgaaat aattaaaaaa
    16441 aacgagggaa tttacaaatg taaactccct cgcaaagatt gtattaacga caaccagcgt
    16501 tctattttag gatgggtttc aaaattggaa tgttgtaata gtatttccct ttttcaaggt
    16561 aaaaacttac taaattctaa tcaattactt tggccaaaaa gtaatttttc ttaccttttc
    16621 gaataaccac aaacttacca tcgaattcat cggatggatt tatgacaaaa tttaaatcgg
    16681 taacgcggtc accgttaata cttatagctc cgtttgaaac atcttcacgt gcttgacgtt
    16741 tagatggttc aatttttgtg gaaacaagca gttccacgat attttcaggt gtgcttgaaa
    16801 tttcaacgtt cggcatttta ccgaatcctt gcgcgatttc ttccgcattt agttctttaa
    16861 tatttccaga gaacaatgct tgcgttattt tttgcgcttc tttcaagtct tcttcactat
    16921 gcacaaatct tgtaacttct tctgccaaac ggcgttgtgc ttcacgtttc tcaggttctg
    16981 tctctacttt tttcgctaag tcttcgattt cttcttgaga caagaaagta aagaatttca
    17041 agtatttcac tacatcgcga tcatcttgat tcaaccagaa ttggtagaac tcaaatggtg
    17101 aagttttctt cggatcaagc caaacggcac cgcctgcagt ttttccgaat ttcgttccgt
    17161 cagccttcag catcaatggg atcgttaaac cgaacgcttc tgcttctggt ccttctaatt
    17221 ttcggattag gtctaatcca gctgtgatat ttccccattg gtctgctcca ccgatttgca
    17281 attgcacatc gtatgtttta tgcagatgca aaaagtcgat agattgcagg atttggtaag
    17341 taaactctgt aaatgagatt cctacttcca aacggctggc aacgatatct tttgctaaca
    17401 tcgtgttgat gttgaagttt ttaccgtagt ctctcaaaaa ttcaagtaaa gagatttttg
    17461 acaaccagtc gtagttgttc acaaatgtga tatttgcatc tttaccaaac aattttctca
    17521 tttgattgga aagtgcgtcc acgttatgtt gcacttgttc catcgtttgc aacacacgtt
    17581 ctgttttgcg gccgcttggg tcgccgatcg ttcctgttcc gccgccaatc agaatatatg
    17641 gatgatggcc tgccaattgg aatcttttca tcatcataaa tggaatcaaa tgtccgatat
    17701 gcatcgaatc accagttgga tcaacgccgc aataaagtga aatgctcttt tcttcaacga
    17761 gctcgcgaag tctttcttcg tttgtttgct gattaatggc gtcgcgccaa gttaattcat
    17821 caataatatt cattttactg acctccgtgt agttttttta tgaaagcagg aacctgctag
    17881 catatgtaat taattatttc ttcaggatag gataggaaat aaaaaaagtc ccttggcata
    17941 taagccaagg gacgaaatct cgcggtacca cccaatttgt atgtctagat atccaacata
    18001 cctctcgaac atttaacgct gtcgcgtggc aatcactgcc aaagactcct gaaatgtaat
    18061 tcgctttaaa gcatgtactg gtttgcagcg accaccagct ttctgaaaca ttggctttaa
    18121 caactacttc gtttcgatct tcgtcttgtt attattaata tgtgtctatc atagagcgca
    18181 actttttctt tgtcaacaga aaaaggatat tttttttaaa ttcctcattt tttctttttt
    18241 gaatccgctc taattccgaa cattttattt tcaattattg ttaaaagaaa taactttttc
    18301 tgtatttttt ctgcaattta tcgagaagtc actttacttt gcctcttatt ctttcttcgt
    18361 tacaataagg aatgggacaa gtaaaaaaat gaaaaagcaa gggacgaggt atatggaaaa
    18421 aaagcaaaaa attattttag gagccacttc tgctttaact atcgttattt taattctaag
    18481 tttgtttttt gtcatccgat catctacaaa aaaagaacca atcattcctg aaaaaaaaat
    18541 aacagaaaaa gcaaaagaac cgacagatga taagcaacaa ctagttactc aagcaaaaaa
    18601 tctggctcaa agctacgatt atgaccaagc gattgcgtta ttggaaaaag atagtaagaa
    18661 cggatctact gaaacacaac agcttcttca aacactaaaa aaggagaaag aacaactgat
    18721 cgactggtct gatcctactc agatctctca tgtctttttc cacagcttga tcgtggaccc
    18781 gcaaaaagcc ttccactctc aacaggcaca aggatacaaa gattatatgg tgactgttga
    18841 agaattcaac cgttcgatcg aacaacttta tcaaaatgat tttgttttag ttaatttgaa
    18901 tgatttgatc caaaaagacg aacaaggaaa tctttccttt aacgggcttg cgctacctga
    18961 aggaaaaaaa ccattggttc tttcacaaga tgatgtcagc tattatgaat atatggataa
    19021 ttccggtttt cctagccgat tggtcgtatc aaataacaat gaaatcaaaa atgtctataa
    19081 agagaaagat aaagaagaaa tcggtgactt tgacatggtt cctctgatcg atgcctttgt
    19141 caagaaacat cctgattttt cttatcgagg cgcaaaaggt accttggccc tgacaggtta
    19201 caacggagtt ttaggttatc gaacatcaaa atctcaatat ggagacaatg aaaaaacaaa
    19261 aaaagagatt aaagaagcta aaaaagtggc ggaacagttg aagaaagacg gctggagctt
    19321 tgcttcccat acatggggtc acttgaatat gactcaagct tcactagaag atgtaaaaaa
    19381 agacaatgaa ttgtggcagc aagaagttgc acctatccta ggaaaaacaa atattctgat
    19441 ttatccattt ggtgcagaca tcagcgactg gcagccttat tcagcagaaa atcaaaaatt
    19501 tgcttacttg aagagtcaag gtttcgatat tttctgtaat gtcgatgctt ctacaccagc
    19561 ttggggacaa ttaggtacgg atttctatcg aaatgctcgg atcaatatcg atggcattcg
    19621 ttttcaatct gatttagacg gaaaaaatcc tattcttgat ccattcatca atgtccatga
    19681 tgtctatgac aaagaagcac gcgaatctgc ttgattttac gtcatttatg aaccaaaaaa
    19741 gccgaactga cattttatcg tcagtttcgg cttttttatg ctttatttct tcttcggtgc
    19801 gattcccttg atcaggactt ctgtccattc tgctgtatat ggaagcagtt ttgcgtatat
    19861 ctccggctta gaaattcctt ctaccattgc ttgaaaatag aggatcaaga actcatccga
    19921 atatttcaaa tccacttttc cttctttccg ccctcgatgg aaaagatcaa gcatgatact
    19981 gaaactttct tgcgtatacg catccatcgc gtcagaaaat tctttttttt ctttttttgt
    20041 atagtatgtc atcaaatcga tataaaattg tttgttcact cgattgagat aacggatttt
    20101 gttttcactc atggcaatca gtgtttcttc aaatggaaga ttttccgcaa tcaacgcttt
    20161 cgcttcttgc cctaaatcat tcataaatgc aaaaaatact tcataaatca gcccttcctt
    20221 gctgccaaaa tatttaaaaa tcgtcgcttt acttacctga gcgttctcag ctaccttatc
    20281 cattgtcact ccagcggttc ctatgtctgt gttcatcaag tcaaaagctg cttgtaaaat
    20341 ctctttcttt ttctcttgtg ctctcttttc aaaaccgttc atttgtagag cctcccgtaa
    20401 ttttaaactt ttataatata tatag
//
LOCUS       DAZKNM010000037        20119 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000037 DAZKNM010000000
VERSION     DAZKNM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.037"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACG8M9_001073"
     CDS             complement(41..1432)
                     /locus_tag="ACG8M9_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377861.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACG8M9_001074"
     CDS             1689..5078
                     /locus_tag="ACG8M9_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0377862.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACG8M9_001075"
     CDS             5081..6502
                     /locus_tag="ACG8M9_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0377863.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACG8M9_001076"
     CDS             6499..8376
                     /locus_tag="ACG8M9_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0377864.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACG8M9_001077"
     CDS             8468..9310
                     /locus_tag="ACG8M9_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0377865.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACG8M9_001078"
     CDS             complement(9462..10337)
                     /locus_tag="ACG8M9_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0377866.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACG8M9_001079"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACG8M9_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACG8M9_001080"
     CDS             11663..11968
                     /locus_tag="ACG8M9_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0377867.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACG8M9_001081"
     CDS             12147..14162
                     /locus_tag="ACG8M9_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0377868.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACG8M9_001082"
     CDS             14164..14448
                     /locus_tag="ACG8M9_001082"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0377869.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACG8M9_001083"
     CDS             14474..15847
                     /locus_tag="ACG8M9_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0377870.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACG8M9_001084"
     CDS             complement(15930..17945)
                     /locus_tag="ACG8M9_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0377871.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACG8M9_001085"
     CDS             complement(17967..19181)
                     /locus_tag="ACG8M9_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0377872.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..>20119
                     /locus_tag="ACG8M9_001086"
     CDS             19401..>20119
                     /locus_tag="ACG8M9_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0377873.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFG"
BASE COUNT         6821 a         3740 c         3786 g         5772 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggcta
//
LOCUS       DAZKNM010000038        20068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000038 DAZKNM010000000
VERSION     DAZKNM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.038"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..419
                     /locus_tag="ACG8M9_001087"
     CDS             123..419
                     /locus_tag="ACG8M9_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0377874.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1063..2016)
                     /locus_tag="ACG8M9_001088"
     CDS             complement(1063..2016)
                     /locus_tag="ACG8M9_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0377875.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2039..2794)
                     /locus_tag="ACG8M9_001089"
     CDS             complement(2039..2794)
                     /locus_tag="ACG8M9_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377876.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2791..3750)
                     /locus_tag="ACG8M9_001090"
     CDS             complement(2791..3750)
                     /locus_tag="ACG8M9_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0377877.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3743..4693)
                     /locus_tag="ACG8M9_001091"
     CDS             complement(3743..4693)
                     /locus_tag="ACG8M9_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0377878.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4843..5295
                     /locus_tag="ACG8M9_001092"
     CDS             4843..5295
                     /locus_tag="ACG8M9_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0377879.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5384..7651)
                     /gene="gshAB"
                     /locus_tag="ACG8M9_001093"
     CDS             complement(5384..7651)
                     /gene="gshAB"
                     /locus_tag="ACG8M9_001093"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0377880.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7838..8836)
                     /gene="ftsY"
                     /locus_tag="ACG8M9_001094"
     CDS             complement(7838..8836)
                     /gene="ftsY"
                     /locus_tag="ACG8M9_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0377881.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8847..9656)
                     /locus_tag="ACG8M9_001095"
     CDS             complement(8847..9656)
                     /locus_tag="ACG8M9_001095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0377882.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9665..13246)
                     /gene="smc"
                     /locus_tag="ACG8M9_001096"
     CDS             complement(9665..13246)
                     /gene="smc"
                     /locus_tag="ACG8M9_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0377883.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13266..13952)
                     /gene="rnc"
                     /locus_tag="ACG8M9_001097"
     CDS             complement(13266..13952)
                     /gene="rnc"
                     /locus_tag="ACG8M9_001097"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0377884.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14300..16081)
                     /locus_tag="ACG8M9_001098"
     CDS             complement(14300..16081)
                     /locus_tag="ACG8M9_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0377885.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16107..17021)
                     /locus_tag="ACG8M9_001099"
     CDS             complement(16107..17021)
                     /locus_tag="ACG8M9_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377886.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(17048..18010)
                     /gene="opp4B"
                     /locus_tag="ACG8M9_001100"
     CDS             complement(17048..18010)
                     /gene="opp4B"
                     /locus_tag="ACG8M9_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0377887.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(18010..18954)
                     /locus_tag="ACG8M9_001101"
     CDS             complement(18010..18954)
                     /locus_tag="ACG8M9_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377888.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18955..19959)
                     /locus_tag="ACG8M9_001102"
     CDS             complement(18955..19959)
                     /locus_tag="ACG8M9_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377889.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5596 a         4295 c         3560 g         6617 t
ORIGIN      
        1 ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag aattactttt tgttcacgga
       61 ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt ggaggaatca
      121 ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat atcgagggtt
      181 cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca ttggatattt
      241 ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc gagtataaaa
      301 tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag cttcccgccg
      361 atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca catgcctaaa
      421 tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt gaacattgtt
      481 tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg acagaaaata
      541 gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat ggaacatcgt
      601 ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg gacagaaaat
      661 agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga tggaacatcg
      721 tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat ctaagtttcg
      781 ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac
      841 tattttttgc cactccccat tctagaatca acccgtatga tgtagataat gaattcacgt
      901 tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata cgattttacg
      961 gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg taagaaacat
     1021 tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa atgcttgatt
     1081 cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat accagacatc
     1141 tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta ccagttcatt
     1201 ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt ctactacaaa
     1261 taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac cgtgagtgga
     1321 tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga aaccgaatct
     1381 agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta gtgctttttt
     1441 gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt cctctaattg
     1501 agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat tttgctggaa
     1561 agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa tttgacttag
     1621 atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct gaagctgatt
     1681 aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt tataggatga
     1741 aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa caccaagagc
     1801 atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat tatttggcac
     1861 ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt gtgtgctgct
     1921 tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta aaccaaatga
     1981 tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa tgttagtttt
     2041 agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc atttcgtata
     2101 attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta aataactgtc
     2161 catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata tcatgaagaa
     2221 caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc atgatttgaa
     2281 ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa tccgtgtctt
     2341 gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga gtgtcaatcg
     2401 attgatccgc tatttcttct aacccaatgt gttccagtga ttcctgaacg atttcctcgt
     2461 cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact aattctttca
     2521 cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt ttggctaatt
     2581 cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct gtatcttttg
     2641 gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt ggaccgataa
     2701 aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact gattttccat
     2761 ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt cgcgaatcaa
     2821 ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg ttgtattcca
     2881 gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag ctaccagaaa
     2941 gcttccgata aatagctgac catgacgata agtagaaaag acacgataac tgatatttgc
     3001 gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc ctgtcaaaag
     3061 actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga ggtttactgc
     3121 ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata acaggtacca
     3181 gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat tgccaaagct
     3241 tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga cttgtaaaaa
     3301 ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta ataaaaaaag
     3361 attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc cgaccatcag
     3421 cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca ctcctccgat
     3481 aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca atactgaagg
     3541 agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc tgacagctag
     3601 cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa aatgccagtt
     3661 tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa caagcatacc
     3721 aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga agataaagga
     3781 agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa ggaaaaatca
     3841 acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa gcagttacgc
     3901 caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga atgatcactc
     3961 cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat gatactaagc
     4021 caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca aacgattcgc
     4081 ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac aatggaacaa
     4141 cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct tgaagccatg
     4201 aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact gaaccaatga
     4261 tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg aggaacaatc
     4321 gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct gtcaaggatc
     4381 ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct aaacgcgcac
     4441 tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa tgttgcatga
     4501 tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt cgtggcaaac
     4561 gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct gacaattgta
     4621 cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat gctactagtc
     4681 gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct tattttaagt
     4741 gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg ggaatatgct
     4801 ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca agatgatgta
     4861 aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga agaacagcat
     4921 aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc tgaaatgatc
     4981 caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga tgcttccata
     5041 ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct agcaacgaaa
     5101 aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga ggaattcgga
     5161 ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga aaaaaaatac
     5221 ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa aagcttctgg
     5281 gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa agcttttgat
     5341 cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat acttcaggat
     5401 ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa ggataaacat
     5461 gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt ttatcagttg
     5521 taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta gctcctagtg
     5581 cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca gtcatatcga
     5641 tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt tgattggctt
     5701 ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct aattcaccta
     5761 attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca ctattttttt
     5821 cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca gggactcgca
     5881 acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt cccggcataa
     5941 attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct gcttgataat
     6001 cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg gttgatttag
     6061 gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct tcttccatgg
     6121 atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact tttttcgtta
     6181 ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc atgtttgcat
     6241 ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc gactcatcta
     6301 atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga gtagaaagtt
     6361 ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta taatattgat
     6421 gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga tagagtttcg
     6481 ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact aaataaagag
     6541 aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt tctctacctt
     6601 cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta tttcgagtcg
     6661 taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac atgtacatca
     6721 ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca taaggattca
     6781 aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct gcgactttct
     6841 ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt ttcccttctt
     6901 cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa actttcacat
     6961 ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt tcttgcggat
     7021 gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat aaataagtga
     7081 tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct gttttgaatt
     7141 cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac tcatcgccaa
     7201 attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat tcttttgcta
     7261 aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg atctcttcgt
     7321 ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct ttcggtatag
     7381 aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga atagaatcgg
     7441 taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata taggggtgat
     7501 aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct gcaagatcaa
     7561 cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg cgcgcattga
     7621 catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat atgaatctaa
     7681 ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt acaaataagt
     7741 ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa acaaggagct
     7801 gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt agtaatcctt
     7861 tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg attccttcac
     7921 caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt acgataccgc
     7981 cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt gtttctttga
     8041 attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca agaagtgttt
     8101 cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct aattcgttca
     8161 tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca tctgcctgct
     8221 ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct cctgcattgc
     8281 cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg attgctccag
     8341 cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg aactgatggg
     8401 caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac aaaataactg
     8461 ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc gcttcataga
     8521 gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc gcatttctga
     8581 gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa cctacatctg
     8641 caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt cgaaaattgg
     8701 caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa cccttgtcgt
     8761 acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca cgcttgattt
     8821 tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat cactttggct
     8881 acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac gaaaggcaca
     8941 gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc attttcctca
     9001 tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa taaagaaata
     9061 ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct tgttttgatg
     9121 atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc taattcttct
     9181 tggtcataag cgattactgc cttattaagc acaaattcat ctgtaatatc gcttatatcc
     9241 gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt ccttggggca
     9301 ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc caattgctga
     9361 gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc catgatttca
     9421 ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc accttcatct
     9481 accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc tgtacataga
     9541 acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc atctgaaatc
     9601 agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt gatcattttc
     9661 gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc atccgtacag
     9721 aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca tctgctgcga
     9781 ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg aaatcgctca
     9841 agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc tcatctaaga
     9901 tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg gctgtaagtg
     9961 ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga ggttgtactt
    10021 caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat tcagctttac
    10081 cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct tcaaatactt
    10141 ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac aattggtttt
    10201 tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca ctgacttgtt
    10261 cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt tcttttaagg
    10321 atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct tgggaagctt
    10381 tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct aaaatcattt
    10441 cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct ttttgctcac
    10501 gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt ctatctcgct
    10561 gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat aattcgttaa
    10621 tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct gtcaatgtcg
    10681 ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg actcgggcac
    10741 cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga tctgcttgtt
    10801 cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg ctttcttgac
    10861 ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct gtttgtttgt
    10921 ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt tcacgtgtct
    10981 caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg atgttttgta
    11041 gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta cgcaaaactt
    11101 cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt ttttcttgtg
    11161 cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat tcttgatttt
    11221 gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg gatcctccgg
    11281 cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc gtttgcgcga
    11341 ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg ttttgaacca
    11401 cagtttggat ttgatcggga taactgactt gttcactggc aatccctata aacccttcta
    11461 cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt gtcgtcagtg
    11521 gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct tgccgagcat
    11581 ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgct tcgatagcta
    11641 acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata cccgaaagct
    11701 gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag ttttcttgga
    11761 tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt aattgataca
    11821 ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt tggatcattt
    11881 ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt tgtgtcaatt
    11941 cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc ttggctaacg
    12001 tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc agttcgtttc
    12061 ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct gccaataatt
    12121 ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga tttgcttcag
    12181 ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct tgaagctttt
    12241 caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct tgcgagcttt
    12301 tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc tgcttcagtt
    12361 tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg gcttgcgcat
    12421 tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca gatagtttgc
    12481 ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct ttttttgctg
    12541 tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct tttagccgta
    12601 aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct tccaattcgt
    12661 ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc tgttctgctt
    12721 tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc ccacgacggt
    12781 cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt gagaacgatt
    12841 cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg cagctttgtt
    12901 tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca ctgatctcat
    12961 tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact tctgcaatat
    13021 tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt ccgcctcgca
    13081 gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata ttgctttttc
    13141 cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg actgtcttgt
    13201 ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct ttccctcact
    13261 tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa ttttttcgat
    13321 ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca aaatactcgt
    13381 tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc acctgatttt
    13441 tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc acctgctttg
    13501 actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc ctggtcaaga
    13561 tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg cgtacgtcct
    13621 cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca ttctttggca
    13681 aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa ttttccttct
    13741 ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa aacggcatct
    13801 cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc attcacatag
    13861 gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat gttatagttt
    13921 tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat ggatattttt
    13981 tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg tcaaaaggta
    14041 agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg tttagtttcg
    14101 agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa tttgacttaa
    14161 taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc agcgccttga
    14221 cctgaatatt agagaaataa acagaaaaat atcaaaaaac cgaaaaagga catcgccctt
    14281 tttcggtcat ttttagtgat tatgaacgtg aatctgctgt aacagaaaca tttgcccatg
    14341 ggtttggtac atcataagac caatcccagt ctttgacacg gtcgttgact ggcaatactt
    14401 cattacggaa caacgttgga atcgcaaatg cttcttcaaa tgcatattgt tgccattttt
    14461 gatatgcttc tttttggtac tcttcatcaa atgctttgct tgaactgata tcgttgatca
    14521 attttgtatt ttcttctgat tcataacgag tgtagttgaa cgcagcattt ggtccccata
    14581 gtccacttgg atttggatca gttcctgtat tccaagcagc ttggtaaaca tcaatttcag
    14641 gatcatcgtt ttctaattta tcatagaaag aattgaattc gatcagacga cctgttgtgt
    14701 attggacatc caaaccaatt tctttccatt gttggatata gtaatctgcc aatggttgcg
    14761 ctgtttcccc accggacatc gaagcaaagt tgattgttag tttatttcca tctttgtctt
    14821 ctcttaaacc atcgtcatcc acatctttgt agccagcatc atctaatagt tttttcgctt
    14881 tgtctagatc ttgtgtgaat cctttcatct catcatcatg gtatgactgg aatactggag
    14941 gaatcaatga agtcgcattg cttctcaaac ctgcatagaa acgttcacct actgcattat
    15001 tatctaatgc atagcccatt gcttgacgta atgatttatc agccattttt gaatctggat
    15061 tgtattccac actctttgta tctgcattcc atttacctaa cttgaatcct agataagtat
    15121 aagaagtttg ttcacggcct aagtttgtat agccagcaat ttctttatag gtatcgtaag
    15181 tatctgttgg catttggaaa atcatatcat ataatttaga gttcaatgct tcagttgcac
    15241 ttgccatcgg tacagatttc atcgtgatct ttttcaattt aggcgtacca ttccagtagt
    15301 gctcgtttgg caagtattct actgattctc ctgcaacgat attactcatg taataagggc
    15361 caaaagtcac tggattttta cgtacttgat cgcttgattc catatctttg attgctacac
    15421 cttctaacgt atgttttggt gctgcatatg accagatacc accacccgat tgaagcatgc
    15481 ttgggtttac ttctttatat tcgatttcaa ctgttttgtc gtctattttt ttgattccgg
    15541 agattgtgtc ggcacttcca tcatgatatt cttccatacc tacgatgtta cggaaagtgt
    15601 cgtcataacg gataccagtg taatctttat tcccaatgat ttcgtaaggg aaaatcacat
    15661 cgtccgccgt tacatcttca ccatctgacc atttgacgtt gtcttttact ttgattgtcg
    15721 cttttttcgt atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg attctgaaat
    15781 cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat aaaagctgca tcatatgaat
    15841 cttggtaaaa ctcccactgg aacaatcctt taaactgact gtccgttgcg accgcagctt
    15901 ctaattcgcc gtcttcgatt gcatcatcat tattttttgt tgcaattgga aaagttaatt
    15961 cctctttgtt attgccgttg cctccagagt tgtttccaga ggtatctgta cctccgcccc
    16021 cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc gttttcttca
    16081 ttcctatttc ccccttcgct tctatactag cctaaccgtt ggcgcgcatc tgcagaacgc
    16141 ttgaatgctt gtccaacata attgatactc aacatcaaaa ctaaaataag gattgatgcg
    16201 ggtaaccaga tccattgccg attcactaag acttctccgt tacttgcata accgatcaat
    16261 gtacctaaac taggtacatt tgccggcaag ccgaaaccta agaaagtcaa agttgtctcg
    16321 ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga acttagattc
    16381 ggcatgattt cgcggaacat gattttaaaa tcattggttc ccattgtttt tgaggcactg
    16441 acataatctc ttcttgcttc agataatgtc ttgcttcgga ataatcgggc tttagctacc
    16501 caataaaatg cactcatgat gaatataaag gaccagacat tgtatctgga aataatcgat
    16561 acgaagacga taatgatcag catgatcggt agaatcatga taaaatcaac gatccgcatc
    16621 aagatgttgt caatgatccc tccgtagtag ccagagatga tccctaatcc aaccccgatg
    16681 atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag agtttctagc acctatgatc
    16741 aattgtccta atacatcacg tccgccttca tctgctccca gaatgaactt ttgtccttgt
    16801 gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac taacatacat cactttgtct
    16861 tgatcggtca gcatcgcacc gataaagatc actaataata aaaccactag caaacctaat
    16921 gaaaaaatag caattttatc ttttttaaat tctcgagcaa tcattcgtat ccccatagga
    16981 ggaatacttt cttgaactgc tgtattgttt ttttcactca tcgatccatt ctcctttctg
    17041 attggtttta ttgaatccga atacgcgggt caacgatact catgatgata tctgaaagta
    17101 atgtccccac tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc acggtatagt
    17161 cccgttggcc gatggaatca ataaatagtt ttccgactcc cggataacca aaaatctttt
    17221 cgataactac agaaccagca atcaacgaag tgatttcata ccccatttga gaagccatcg
    17281 gcaacgctgc atttcggaaa atatgtcgac tgaatacttt attcgtcggt acccctttgg
    17341 aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt cgcagatatt
    17401 ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga atatatttca
    17461 aacggtctat ccattgagtg ataaatccac cttgcaaacc tagtgtttgt gatccacttg
    17521 ttgggaacca gcctaaacga taaccaaaaa caaatagcat gatcaagccg aagataaata
    17581 aaggaacagc aaaactaaag aagttgtaga ttacgacaat cttatccaag attgaatttt
    17641 gataccggcc agcaagcatc cccagaggca cagcaatcaa ataagtaagg acaactgtca
    17701 agatcgatag atataaagtg ttgatgatcc gttcgccgat caagtcagca acagagcgtt
    17761 tgtataagaa actttgacca aaatcgcctt gcaaagcatt tccgatccaa cggaagtact
    17821 ggacatacca tgggtcattc aagccagctg cttgtcgcat ctgttcgatc actgccggat
    17881 cctgatttgg attgatcaaa ccggtaaatg gatcccccgg catcgcttgg gctaataaga
    17941 agatcagaac acttaaaata ataacttgcg ggatcatcaa taaaacacgt cgcagaatcg
    18001 ttttccacat tagcttgcac ctccatcttt caacgccact ttgtgtgtag ttgtgatcgt
    18061 acgcaaatcg tagactcgac cagtcgcatc atagtattct ttttggtttt ggatatactc
    18121 ttgttctact cttcgtcgat tttctttgtg gctttcccta ttttctacat cgatttgcgg
    18181 aatagctgaa agcagacgtt tcgtatagat atgtcgcggg tcattataaa tgtcttctct
    18241 tgtaccgatt tctacaaatc gacctttgta cataattgcg atattgtcgc acatgtgttt
    18301 tactacacct aaatcatgtg aaatgaataa atagctgagg ccgtattctt gctggattcg
    18361 tttcatgaaa ttcaatactt gcgcttgaac agatagatcc agagcagata caggttcatc
    18421 ggctacgatc agttttggat tggtagcaac agcacgggct actcctagac gttgacgttg
    18481 tccgcctgaa aattcatgag gatatttata tagcgcatct tccggcatcc caacgatatc
    18541 tagcagtcct tttacctttt tcttttcttc ttgatcgctt aaccgttcaa aatttcggat
    18601 gggttcagca atgatatcca acactcgctt tttaggattc aaactggaca ttgaatcttg
    18661 gaagatcatc tgcacatcct tgttataacc catttttttg cggttgcttc gcttcgttac
    18721 atcttttcct tcatacatga tttgtccggc tgttaccttt tctaatccca caacggcttt
    18781 tcccgtagta gattttccag agccagactc tcccactaaa ccatatgtct ttcctttttc
    18841 gataataaaa tccacaccat ctacagcata tacatgatcc gttactcgat tgaagaatcc
    18901 actgcgaata gggtaatgaa ctttcaggtc tttgattgtg attaattctg tcatctacac
    18961 ctctcctttt tcgtctctaa agtggaaatg cttatagcag gagcaacgta ccaagtggtt
    19021 cggtgcgact tcatgaagga taggctcttc ttcatgatcg cttgctggta tccatggaat
    19081 acgtggtgca aaccgacaac ctttacgagg catgtttttc agagaaggaa cgacaccttc
    19141 gatcacatgg agttctgcat catgatttcc ttcttgtggt atagagttca gtaaagagcg
    19201 tgtataagga tgcttaggat gatcaaacag ttcttttaca cctgctactt caacgaactg
    19261 tccgccatac atcactgcca ctcgatcagc catttctgct acgactccta agtcatgagt
    19321 gatcaagatg atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat caagaatctg
    19381 tgcttggatg gttacatcta gggctgtcgt cggttcatct gcaatgacga ttggaggttt
    19441 gcaggcgatt gcgattgcga tgatgacacg ttgtctcata ccgcctgata gttcatgggg
    19501 atattggcgt ccaactctcg caggattagg aatccctact tgttctagca attctagtgc
    19561 acgagcttga cgctgttctg cattcatctt tgtgtgatag gaaagtcctt ctttgatctg
    19621 gtcttcgatt ctcatgagag gatttaatgc agacaacgga tcttggaaaa tcattccgat
    19681 atcatttccg cggattttat tatagagcgt ttcgttcagg tcgattaaat tcagatcgtt
    19741 atataaaatc tcacctgtaa tttttgtgtt attaggatca agtaatccca taatcgtagt
    19801 agccaaggta cttttcccgc acccagattc cccaacgatt gctaaaatct cgtttttgtc
    19861 taaagtaaat gaaacgtcat ctactgcatc gtaatagtca tctttgagtc ggaatcctgt
    19921 atgtaggttc tgaacttcta ataattggtt atcggacaat cgaatacccc tctctgcttc
    19981 ctaaatggaa gtttttctta ataaaaaagg acattacaca gaataaatcg ttttataaca
    20041 aaggttattc ttatctttgt gtaatgaa
//
LOCUS       DAZKNM010000039        20056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000039 DAZKNM010000000
VERSION     DAZKNM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.039"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1588)
                     /locus_tag="ACG8M9_001103"
     CDS             complement(29..1588)
                     /locus_tag="ACG8M9_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0377890.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1743..3977
                     /gene="pcrA"
                     /locus_tag="ACG8M9_001104"
     CDS             1743..3977
                     /gene="pcrA"
                     /locus_tag="ACG8M9_001104"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0377891.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            3994..6030
                     /gene="ligA"
                     /locus_tag="ACG8M9_001105"
     CDS             3994..6030
                     /gene="ligA"
                     /locus_tag="ACG8M9_001105"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0377892.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6136..6441
                     /gene="gatC"
                     /locus_tag="ACG8M9_001106"
     CDS             6136..6441
                     /gene="gatC"
                     /locus_tag="ACG8M9_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0377893.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6441..7913
                     /gene="gatA"
                     /locus_tag="ACG8M9_001107"
     CDS             6441..7913
                     /gene="gatA"
                     /locus_tag="ACG8M9_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0377894.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7910..9340
                     /gene="gatB"
                     /locus_tag="ACG8M9_001108"
     CDS             7910..9340
                     /gene="gatB"
                     /locus_tag="ACG8M9_001108"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0377895.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9350..10405
                     /locus_tag="ACG8M9_001109"
     CDS             9350..10405
                     /locus_tag="ACG8M9_001109"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0377896.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10516..11889
                     /gene="rlmD"
                     /locus_tag="ACG8M9_001110"
     CDS             10516..11889
                     /gene="rlmD"
                     /locus_tag="ACG8M9_001110"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0377897.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11892..12020
                     /locus_tag="ACG8M9_001111"
     CDS             11892..12020
                     /locus_tag="ACG8M9_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0377898.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12460..12648
                     /locus_tag="ACG8M9_001112"
     CDS             12460..12648
                     /locus_tag="ACG8M9_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0377899.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12726..13739
                     /locus_tag="ACG8M9_001113"
     CDS             12726..13739
                     /locus_tag="ACG8M9_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0377900.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13806..14840)
                     /locus_tag="ACG8M9_001114"
     CDS             complement(13806..14840)
                     /locus_tag="ACG8M9_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0377901.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            14955..17180
                     /locus_tag="ACG8M9_001115"
     CDS             14955..17180
                     /locus_tag="ACG8M9_001115"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0377902.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17198..19081
                     /locus_tag="ACG8M9_001116"
     CDS             17198..19081
                     /locus_tag="ACG8M9_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0377903.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19062..20036
                     /locus_tag="ACG8M9_001117"
     CDS             19062..20036
                     /locus_tag="ACG8M9_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0377904.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6747 a         3689 c         4314 g         5306 t
ORIGIN      
        1 ttgttgtaaa atagtttttc catgttcgtt aaaatgattg cttcaaattt gtgatagaag
       61 atttagcctc gatttcctgc ttgattcttg gcaagtttgc ttcatcagtc aagataacca
      121 aaatatctcc tacattcatg atcgtatcac cgtgagtcaa gatttcctga tttcctctgc
      181 gtatagagat tagcagcata tttttaggcc aattgaagtc tcgtaccatt aatccgtcta
      241 aagaactctc tgccgttact ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg
      301 tctgagtatt tccaagtaga cgttccaata atgattcgta aattggatta ccgccgagaa
      361 ggtcattgac gatataagca acaagagcag ccaatcctaa aggcatcaga tgattaagag
      421 agccaaccat ttctgtcact aaaataattg ctgtcagcgg tgcttttcca atcgctgtga
      481 aataaccagc catggcaata acgacaaaat ccttgatgta gattggatcc atcccgaaat
      541 caaccataat gtttccataa aagctaccga ttaaagcccc caatgtcaat atcggaagga
      601 aaatcccccc tggaagaccc ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa
      661 acaaaccgat aatcataatg attggcaagg aagcatgtcc aaaagctaaa acgatttgat
      721 tgcctccgcc tagagtacta ggccataaat aaccaattgg caaaatcagc aagaaaggaa
      781 cgaacccgtg aaaataagct ggtaaaggca atcggttgta aaattttggc aaagccagca
      841 agacattctg ataaataaaa cctaagatgc ctagaagtac tcctaagatc actaaggaac
      901 cataatattt caacggcaaa gaaggaaggt cacctagata taaaacaggt ctcaagccga
      961 aaaagtaaag agagacgaag ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa
     1021 gtggagaaaa ggagtgatgg atttcttcta aaacaaagag taatcctgcg ataggagcat
     1081 tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat taaaatcttt tcttctgaag
     1141 atggactccg aaacaattgt ccagctcctt gtccaatgga tgcgccaagc tgtatagaag
     1201 gtccttctct acctagaaaa aggcccgcac caactgaaag tacaccgcca atgaactttt
     1261 tccataaaac agagaaccag ttcattgata attccccgcg aagctgtccc actacttgtg
     1321 gaatcccgct tcccttgata tccggttcat ttttgaccag ataacccacg aaaaatgctg
     1381 caaggatgca gcaaatagct aggccaatta agtaaatcgg ctgttcccga ctcatttcat
     1441 agatacttcg aactaaatcg cttagctttt cgatagataa tcgaaacagg ctaacgacga
     1501 ttcctgtcag cagtccaaca aatattcctt ttagaataaa atatatctga gtcttatcta
     1561 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
     1621 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
     1681 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
     1741 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
     1801 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
     1861 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
     1921 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
     1981 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
     2041 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
     2101 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
     2161 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
     2221 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
     2281 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
     2341 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
     2401 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
     2461 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
     2521 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     2581 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     2641 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     2701 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     2761 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     2821 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     2881 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     2941 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     3001 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     3061 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     3121 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     3181 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     3241 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     3301 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     3361 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     3421 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     3481 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     3541 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     3601 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     3661 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     3721 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     3781 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     3841 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     3901 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     3961 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     4021 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     4081 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     4141 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     4201 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     4261 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     4321 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     4381 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     4441 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     4501 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     4561 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     4621 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     4681 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     4741 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     4801 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     4861 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     4921 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     4981 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     5041 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     5101 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     5161 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     5221 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     5281 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     5341 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     5401 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     5461 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     5521 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     5581 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     5641 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     5701 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     5761 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     5821 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     5881 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     5941 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     6001 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     6061 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     6121 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     6181 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     6241 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     6301 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     6361 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     6421 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     6481 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     6541 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     6601 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     6661 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     6721 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     6781 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     6841 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     6901 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     6961 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     7021 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     7081 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     7141 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     7201 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     7261 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     7321 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     7381 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     7441 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     7501 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     7561 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     7621 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     7681 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     7741 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     7801 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     7861 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     7921 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     7981 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     8041 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     8101 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     8161 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     8221 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     8281 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     8341 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     8401 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     8461 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     8521 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     8581 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     8641 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     8701 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     8761 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     8821 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     8881 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     8941 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     9001 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     9061 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     9121 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     9181 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     9241 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     9301 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     9361 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     9421 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     9481 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     9541 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     9601 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     9661 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     9721 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     9781 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     9841 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     9901 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
     9961 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
    10021 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
    10081 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
    10141 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
    10201 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
    10261 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
    10321 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
    10381 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
    10441 cggttttagt tgtgtaatta gctgatttat agtagagttc agtaggattg aaaaataggc
    10501 ttaaaggagc aaaatatgac agaacagcaa atagtaaaga aaaacgaacg atatacagtc
    10561 gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa aaatcgatgg ttatccatta
    10621 tttatcgaaa atgcactccc tggagaagaa gtagaaatac tagtagtaaa agcaggcaat
    10681 aagtttggat atggaaaagt cgaaacattt attacacaat cacctgatcg tcagcctgta
    10741 gaaaacaatg cattacttcg cataggtatc gcacctttag cccatttgaa gtatgaacaa
    10801 cagcttgtct tcaaacaaaa acaagtagag catgtaatgg cgaagattgc taagatgcca
    10861 gaagttcctg tacttccaac ggtcggaatg gcagaaccat tcggctaccg taataaagca
    10921 cagatccctg ttcgccgtat cgatggacag ttagccaccg gtttttacaa aaagaatagc
    10981 catgatctag tccctatcga agatttttat atccaagatc ctgtcatcga tcaagcaatc
    11041 aaagtagtca gagatatctt gcaacggttc caagtacgag gatataatga agaaaaaaat
    11101 gaaggacagc ttcgtcacat cattatccgt cgtggtcatt attcccatga aatgatggtc
    11161 gttttagtta ctcgtaaaga aaaattcttt aaatccaagg aaattgctga agctattcat
    11221 gaagaattgc cagaagtggt ttcggtcatc caaaatatca atgaagaaaa aacaaatgtg
    11281 attttaggag acaaagaaaa agttttatat ggcagagact atatcgaaga tcagctatta
    11341 ggaaaaacct atcgaatctc agcaaagtca ttttatcaag taaatactca acaagcagag
    11401 tatctgtacc aaacagccat cgagtttgcc gagttagata aaaaagacat tgtgatcgac
    11461 gcttattcag gaataggtac gattggcttg tctttagcag acagagtgga taaggtgtat
    11521 gggatggaag ttattccaga agcaattgaa gatgcacagt tcaatgcttt aaataataaa
    11581 atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag ttatgaaaaa atggcaagaa
    11641 aaagggatca aaccatctgt gatcgtagtt gaccctccac gaaaaggatt agatcatcat
    11701 tttattgaat cagcaatcga aatgtcacca gaacgaatcg tctatatctc atgtaatccg
    11761 gcaacttttg caagagatgc taagttattt gcagaagcag gttatgccgt gaagaaagtt
    11821 cagcctgtag atcttttccc gcaaacccat catatcgaat tagtcgcttc attcgttaaa
    11881 ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt gatcaaaaag aaacaaggtg
    11941 gaggccgatc caaacaaatg gaaacaccta agaatgatcg taaaaacatg agaaaaggcc
    12001 ctaaaatctt taataaatag acgtgctaaa gaaagactaa gaagtgttaa cttttgttat
    12061 tgtttcttag tctttttagt tagtctacat tattttggcg atttgccaat aaggactgtt
    12121 gagttgtgca aaatcaaatc tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt
    12181 atttcaattt gattaactga ttgatcaaaa agatttttat tttcatattc tcaaatgatt
    12241 caacactaaa tatacatgtt tgacgcggaa attattattc gattttggcg gcatcagggg
    12301 ggatgacgac gtagcagacc cattatttta ctcaagaaaa tgtccgtgcc tattatttag
    12361 aatacgatag tgaacgtgct gggagttttg ttcttttaga gaaagtctct gaagataaat
    12421 tagttgtctt agattttgac tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg
    12481 attgaccgga tctacgaagc gacacgatat ctgcctttgg atcgtttgtg cttaagtcct
    12541 caatgtggat ttgcttcaac agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa
    12601 tttcttttgt caaaagcatt gcagaagaag tttggggaaa atcattaaat catctaggag
    12661 agcttgatag gaaataacac tatggtataa tcagaatgct taatactaac tactgaggga
    12721 gccttatgaa aaagaaaatt cttaaattta tcggatggtt cttcggaatc attcttggga
    12781 tcgcgtttgc aatctttctt gccttccagc tttcccccag acctggagca ttcttgatca
    12841 accatatgtt ttcaaatact gttcaaatca ctgataaaaa aacgttcgat caagcagaaa
    12901 aaaacgtggc gaaaaaaaca gatttgacgt atgagtccaa gtataaagag aatacttatg
    12961 acatctacta tccaaaaaac agcaaggggc ctgttcctgt tctattttgg gtacatggtg
    13021 gaggattcgt cggcggagat aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag
    13081 acgccaacat cgcagtggtt gcgatgaatt atgaattagc gcctgattca gaatacccta
    13141 atcaagttct tcaagtgaat gaactgatca aagatttatt agctgaagaa aaagacgaca
    13201 aattgctaga catggaacaa ttattttttg gtggagatag tgcaggttca caaatcgcat
    13261 tgcaatatgc agctatacaa acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc
    13321 tacttccaaa agatagtctg aaaggaacca tttcttattg cggacctgtt gatttgaagc
    13381 taacagcaca gcagcattcg gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt
    13441 ggtcgttgat tggaacaaaa gattggaaaa cgagtgagca attgaaagaa gctagtgtgg
    13501 ctgaccatgt ttctaaggaa tttccgccaa cgtatataac tgacggaaat gcatactcat
    13561 tccaggagca aggtcaagca ttagaaaatc ggttgacaga attaaaggta cccgtgcaat
    13621 ccttgtttta tacagataca aaaaaagaga ttacccatga ataccaattt gattacacgc
    13681 taaaagaatc gcaaaattgt taccagcaga cattggattt tgtgaataag tataagtaag
    13741 aggttgtgaa aaagccgttt cgttttgagt atgaagaaac gaagcggctt ttcacaacct
    13801 ctctttcaat acctttttgt tcttgggata aaactttccc taacaatcaa tcgtgcagga
    13861 taagtcaaaa tgattggttc gtcccgtata ccgtcaattc gctccaatgc cattcgaaca
    13921 gctgcttgcc caattgattt cgcattgatt tgaacactgg tcaagccagg ggagaggttt
    13981 gcgattgtct cgatattatc aaagcttgct acaacaatct cttctccaat ttggatattt
    14041 ttttgtttca atgcccgata gacaccaatt gccattggat cgctcccgac aagaatggca
    14101 gtaggaagag gggattcatt ttctaataga ttagtcgcaa gtacctcacc ttgtttttct
    14161 gtccagccac ctagatgata agacatatat ttttctaagt gatgatcgat cataaaatgc
    14221 tgataggcac gcatcctctt ttcatttata gaaatctgct tcccaccgaa ttcatccata
    14281 tcgactttat atcccccgat ataggctatt cttttatgac cattttctaa gaacaattcc
    14341 agtacggttt tagtcatccg ttcaagatct gcatacacca tatcgacctc ttgctggatg
    14401 tcagggttat ccacgacgac tatttgcatg ttttgtttta acaaggcggt aatcgattct
    14461 ttcgtaacag tcccaacaac gatcaccgca cctaactgat ctagatcttc ccattgttta
    14521 ggattatcag atagattata gattcgattc atccctaaat gcagacgtcg agcttcttct
    14581 tccatcccta ctcgcaaatg tttgtaatag atatcctgtt cttcgctcgc tttgccgatc
    14641 gtagagatca caccaatcga aggcattctc tttttgatat atttacgagt actgatgtca
    14701 taatccaacg cagcagctgc ctctactatc cgttgccgag tagtctgagc cacatggatc
    14761 gtctgatcgt tattcaacac acgggaaact gtagcaggag agacatttgc ctttcgtgcg
    14821 acatctctaa ttccaaccat ttttacacac tcctcaaaaa aataaaaaat ttttattgac
    14881 aaaattgttt agtaaacagt atacaggaat tataaaaaaa agtaaacggt ttccgttttg
    14941 ttgggagtga aatgatggat attacgcata gaaaaacaga taaaaatttc atcttatttg
    15001 atgaaaagag ccgagtcttc catttacgaa atacatttct ttcttatctg ataaagatag
    15061 aagaaagcaa tgtattggcg catatttatt ttggcaaacc agtcaagcag tacaaagaca
    15121 ataaaaatta tccccgaaga gatcgtggat tttcaggaaa tgttccctta aatcctgacc
    15181 ggtctctgtc gaaagataca ctaccgcaag aatactcttc tcatggcagc atggactttc
    15241 gaacgcccgc atcgatcatt cagcgcaaga atggatcaga cttactggat ctgagatatg
    15301 acagccatta tattacagat gggaagccag agatcgaagg cttgcctcaa acttatgtga
    15361 tggacaagtc agaggcacaa acgttggtga tttcacttaa ggatagagaa acagcgattt
    15421 atttcgattt gttctatacc atatttactg atagagcagt gatcacacga tctgtaaaga
    15481 taagaaatga aaccggagaa acaatcaaac tagaaaaagc ggctagtttc cagctagact
    15541 ttgcccatac taggcgattt gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa
    15601 gacagatttc acggcaatct gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa
    15661 cgagcagtca ccatatgaat aactttattg ctttagttca tcatcatacg acagaaaata
    15721 ctggagaagc gatcggactt caatttgttt attccggcaa ccattctttc gaacttgaaa
    15781 aagatcagat caatcaattg agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg
    15841 aattgaatgc aggccagtct tttcaaacac ctgaaatgat cctttcttac tcaagtcagg
    15901 gattgaacaa aatgagtcag atccaccatg agttgctacg tgaaagaatc gcaagaggtc
    15961 gtcatcaatt tgctgaacgg ccgattctgg tcaataactg ggaagccact tactttgact
    16021 tcaatagtga aaaaatcaag gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt
    16081 tcgtattaga tgatggctgg tttggaaaaa gagatgcgga caattcttca ttaggagact
    16141 ggttcgagta tgaaggcaag ttgacaaatg gattacgtga aatagcagat tatgctcata
    16201 gcaaaggatt gaaatttggt ttatggtttg aacctgaaat gatctcggtt gattctgaac
    16261 tgtatcgaac acatcctgat tttcttatgc aggagccagg tagaatgcct tctgcttcca
    16321 gatcacaaca tgtactggat tttacaaggt tagatgttcg gcagacgatt gagaaacaaa
    16381 tgagaaagat tttagatacg attcctcttg actacatcaa atgggatatg aaccgttcgc
    16441 tttcagatgt ctattcgatt acccttgacc cacagagaca aggagaagta gcgcatcgtt
    16501 atatgctcgg gctttatgaa ctacttgaac atttgacaac tgattatccg gaaatcttat
    16561 gggaaggctg ttctggtgga ggtggacggt ttgatgccgg gtttatttat tatatgccgc
    16621 agtcatggac aagtgataac acggatgcag tggagcgtat gaaaatccaa tatggaacaa
    16681 gccttgctta tccgatttcg tccatcacag ctcatgtatc agctgtacca aatcatcaga
    16741 ccgacagatc tacttcgttg aaaacaagag gagaaacagc tatgagcgct gtttttggtt
    16801 atgaactgga tttgacaaag ctgtctcctg aagaagaaaa gcaagtaaaa gaccaaatta
    16861 tatcctatca aacgattcgt ccggtgatcc agtatggcca ttattatcgt ttggcgagtc
    16921 cttttgaaga aaatattgcc gcatggatgt ttgtgtctcc taaacaagat gaagctattg
    16981 tgtttttagg gaggatacta gcttcagctc aaccagcgtt ccatgaagta tatctgatgg
    17041 ggttagatga tgaggcactt tatcaggaac agacctcgaa gcggatattt tcgggggccg
    17101 aattgatgac agttggactt tacttccccg attttcaagg tgatttccaa acagaactgc
    17161 ttcatttcaa aaagttatga gagagaagga aaaaagtatg aaagcaaata taaaatggtt
    17221 agatgatcca caagttttta gagtgaacca atcaaatgca catagcgatc atgtttgtta
    17281 tgcaacaagg gaagaagtag aaaccacatc ctcccttaaa cagtctttaa atggatcatg
    17341 gcgtttttgt tattcgccaa atccttcggt acgaccaaaa acattttata aaaaagattt
    17401 tgatgcctgt tcttttgatg aaatcactgt tcctagtcat attgaattag caaattatga
    17461 caagatccat tacatcaata cgatgtatcc gtgggaggga catgtatttc gccgtccccc
    17521 tttcacgaca agccctgaga aagaggcgga agggatcttt tcagctgcta aagacaatcc
    17581 ggttggttcg tatttgacga cttttcgatt agaagaaggt ctaaagggaa aacaaatctc
    17641 gattcatttt gctggtgtag aacaagcaat gtttatttgg ttgaatggtc attttatcgg
    17701 gtatgccgaa gattcgttca ctccgtctga atttgattta acaccttatt tggaagaaga
    17761 aaatattcta gctgtggagg tttacaaaag aagtacagct gcattcttgg aagatcagga
    17821 ctttttccga ttttttggga tcttcagaga tgtcttctta gttgctcgag gacaaaacca
    17881 tttagaagat ctttggatca aacctcgctt agatgacaaa tgtcaaaatg gcaaacttga
    17941 agtagatttg tcttttgagc aagtagccaa aaacagtcag ctgaatgtag cgatactaga
    18001 tcctgatggg aaggaaatct atcaacagca atatccgtta aaagagcaaa tgacgattcg
    18061 catcgatgga tttgaaaatg tggcactgtg ggggcatttt cagcccaatt tatataagct
    18121 tgaattggag acgatagacg aagaaaaaag agtaacggag tatattgact attccttcgg
    18181 ttttcgtaaa atcgaaatca aagataaaat catctacttc aatggaaaac ggatgattat
    18241 caacggggta aatcgacatg agtggcatcc gaaaaaagga cgcgccatca ctatagaaga
    18301 tatggaaaaa gatttggcta tcataaaaaa tgcaaagatc aacggcgtac ggacatctca
    18361 ttaccccaac cagatcactt ggtattattt atgcgaccaa gcaggaatcc atgtgatggc
    18421 ggaaacgaac ctcgaatccc atggctcatg gcaaaaaatg ggcgcatttg agccttctta
    18481 caacgtacca ggtgcagtac ctcagtggca ggcggctgtt cttgatcgag caaaaacgaa
    18541 tttcgaaaca ttaaaaaacc atccaagtat tcttttttgg tctcttggaa atgaatctta
    18601 tgcggaagaa aacattcgac aaatgaacga ttacttcaaa aaagtagacc ccgatcgact
    18661 tgtccattat gaagggaatt ttccaaatcc agcattcgaa gattcgattt cagatgtaaa
    18721 aagctgcatg tatgcgccac cagaagagat cattgactac cttgaaaata aagcggaaaa
    18781 accgttcatc ttatgtgaat atatgcatga catggggaat tcgttaggtg gaatggattc
    18841 ttacatgacg ttgcttgatc gttatgaaat gtaccaagga ggctatatat gggattttaa
    18901 agatcaggcg atccaagtga ctgatccaat cacaggcaga gaagtgatgc gctatggcgg
    18961 tgactttgac gataaaccaa gtgactatga attctcaggg aatgggatcg tttttgcaga
    19021 tggacaagaa aaacccgcca tgcaggaggt aagatattat tatgaaaaat acagtaaata
    19081 aaagtcatat ggatacggaa aaagttgcaa tcgtcttcgg cgactgtaca ttaggtgtca
    19141 aatcggggaa tacgcattat attttttctt atacaagagg cggactggaa tcgctcaata
    19201 aaaacggaaa agaatggcta taccgggaaa cacttccagt tttttggcga gcaacgaccg
    19261 ataatgacag aggtagcgga tttggattta aatccggtat gtggttagga gccggattgt
    19321 atccaaaagt caaagaaata gaagtactgg tggacaataa aaagatcgat ttgccgattg
    19381 ctccagtaaa taatcaatat tcaagtaatg aatacgctga aacggttgaa atcattttta
    19441 cactggaaat caacacagta ccaaaaaccc aagtgacaat ttcctaccaa atcgctggag
    19501 agggtaaaat gcttgtcaat atgcattatt atggacaaaa aggattaccg gaattacccg
    19561 cattgggttt aaggttcatt atgccaacga aagcaaaatt ttttgagtat gaaggcctat
    19621 caggggaaac atatccggat cggatggctg gtggtgtcga aggtatttat aaagtggcag
    19681 gactccccgt ttccccatac cttgttcccc aagaatgcgg catgcatatg gaaacgaaat
    19741 gggtagaact aacaagaaat caaacgttgg ataatacgga taaagatcaa gaagattttt
    19801 cgttgcgatt tgaacagcag gatacgccat ttgctttttc cgctttacca ttcaaagcag
    19861 aagaactgga acaggcaaca catatagaag aactaccgcc agaaagacga acggttctta
    19921 cgatttttgc aaaagtaaga ggagtcgggg gtatcaatag ctggggagcg gatgttgagt
    19981 caccttaccg aatcagcagc gaagaggacc atgaaatcgg ctttattatt cagtagcaat
    20041 ctttagatta ttattt
//
LOCUS       DAZKNM010000040        19817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000040 DAZKNM010000000
VERSION     DAZKNM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.040"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..196)
                     /locus_tag="ACG8M9_001118"
     CDS             complement(<1..196)
                     /locus_tag="ACG8M9_001118"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0377905.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(342..1220)
                     /locus_tag="ACG8M9_001119"
     CDS             complement(342..1220)
                     /locus_tag="ACG8M9_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0377906.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1259..2377)
                     /locus_tag="ACG8M9_001120"
     CDS             complement(1259..2377)
                     /locus_tag="ACG8M9_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0377907.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2374..4473)
                     /locus_tag="ACG8M9_001121"
     CDS             complement(2374..4473)
                     /locus_tag="ACG8M9_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0377908.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4524..5345)
                     /locus_tag="ACG8M9_001122"
     CDS             complement(4524..5345)
                     /locus_tag="ACG8M9_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0377909.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5342..6151)
                     /locus_tag="ACG8M9_001123"
     CDS             complement(5342..6151)
                     /locus_tag="ACG8M9_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0377910.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6172..6666)
                     /locus_tag="ACG8M9_001124"
     CDS             complement(6172..6666)
                     /locus_tag="ACG8M9_001124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0377911.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6678..7178)
                     /locus_tag="ACG8M9_001125"
     CDS             complement(6678..7178)
                     /locus_tag="ACG8M9_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0377912.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7243..9939)
                     /locus_tag="ACG8M9_001126"
     CDS             complement(7243..9939)
                     /locus_tag="ACG8M9_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0377913.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10367..11440)
                     /locus_tag="ACG8M9_001127"
     CDS             complement(10367..11440)
                     /locus_tag="ACG8M9_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0377914.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11437..12264)
                     /locus_tag="ACG8M9_001128"
     CDS             complement(11437..12264)
                     /locus_tag="ACG8M9_001128"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377915.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12265..13071)
                     /locus_tag="ACG8M9_001129"
     CDS             complement(12265..13071)
                     /locus_tag="ACG8M9_001129"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0377916.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13083..14168)
                     /locus_tag="ACG8M9_001130"
     CDS             complement(13083..14168)
                     /locus_tag="ACG8M9_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0377917.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14185..14727)
                     /locus_tag="ACG8M9_001131"
     CDS             complement(14185..14727)
                     /locus_tag="ACG8M9_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0377918.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            14985..15974
                     /locus_tag="ACG8M9_001132"
     CDS             14985..15974
                     /locus_tag="ACG8M9_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0377919.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15971..16708
                     /locus_tag="ACG8M9_001133"
     CDS             15971..16708
                     /locus_tag="ACG8M9_001133"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0377920.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16705..17256
                     /gene="coaC"
                     /locus_tag="ACG8M9_001134"
     CDS             16705..17256
                     /gene="coaC"
                     /locus_tag="ACG8M9_001134"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0377921.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17272..17904
                     /locus_tag="ACG8M9_001135"
     CDS             17272..17904
                     /locus_tag="ACG8M9_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0377922.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            17989..18636
                     /locus_tag="ACG8M9_001136"
     CDS             17989..18636
                     /locus_tag="ACG8M9_001136"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0377923.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18641..19624
                     /locus_tag="ACG8M9_001137"
     CDS             18641..19624
                     /locus_tag="ACG8M9_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0377924.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         6069 a         4256 c         3457 g         6035 t
ORIGIN      
        1 accaaaaaaa ttcaaacatt aagccaagga ttatgaagtc aacatttcga aaaatgaaca
       61 tacattggta agcaactata actattacca cagacataaa gtaacacaat atattactat
      121 ttaagtgtac gccaccagtg aatatcctca aggtatacag cgcacaacaa aaagaaaaaa
      181 ggaaacgcag aagcatttcc tttcagtgat ttaatgaaaa caacacgatt tgacttagag
      241 cctttttatt tgaattcatt ttcgctttat ccaacttcat ttgatcattt gattactcgg
      301 ttaaatactt ttgacatgag tatcaatgaa acattcatta attatggttg cttatttctt
      361 accgcttttc tggccaatgc taatccgccg attattccgg cattatcacc taattcgggc
      421 gtaataatgt agctatcaat tggtggtatt tcaacatatc catttataag ctcaacaaat
      481 ttatcctgaa cttttttctt catgtgttct tgtttcatta caccaccgcc aaaaataatt
      541 ctatctggcg aaaacatcag tgttgtattg tatgcacatt gggcaaggta aaaggcttca
      601 atctgccaaa acgaatcgtc agccaataaa ttctggcctt ttacacccaa ccgtttttca
      661 atcgctggtc ctgctgccat cccctctaaa caatcttggt ggaagggaca gtttcctgaa
      721 aaattgtcct ttggatgtcg cttgatcaac atatgcccca tttccggatg actgaaccct
      781 tccacgaaag tcccattgaa caaggcacca cctccgatac ctgttcccac agtgaagtat
      841 gcaacacctg ctaacccttt gccagaacca gcaacatatt caccatagca agccgcattg
      901 acatctgttg tccattcaat cggaatattg aaatattgct ttagcgttcc tacaaaattg
      961 aaattttgcc aagcaagttt tggtgttgat gtgatatgac cgaaactcgt tgatttgcat
     1021 tgaatatcaa ttggaccaaa gcagccgacc ccgatgcctg ccaattgttc cttaaaaggt
     1081 ttaaaaaaat caatgacctg cttcattgtt tcaactggag ttgttgtggg aaaactgact
     1141 ctttccaaaa tcgttaattc ctcagtaccg actgcacaga caaatttagt ccctccagct
     1201 tcaattgccc catatatcat cataggttcc tcccttcgaa tatgtgaaaa acacttcatt
     1261 aacgcggcaa gcgttctcga tcagtgaaat gacgtttagt atttcctgaa gcttctgttg
     1321 aataccagta agcgacacta gcaacatcgt cttgccgctc aaataagcga acatcatcgt
     1381 tgccgatttg ttgtaaagtg accgataaat cttcggtaaa acagatgggg tcttgaagat
     1441 gccaacgata aagaccatgt cgtggcattg cgtcgtcacc aaaggcatga acggcattca
     1501 attttcccgt ttcaaaacga tctcttgtgg catctctatt tgattggtaa ggatacccta
     1561 aaaaggctgt tgaatagttt ttcgcatgtg gacgtccgaa ctcatcgtac tcatgaaagg
     1621 cccacgcccc gccaaaataa tcttctgaac ctgttgaggt tacggtcgga tactcactat
     1681 ccccatcaat gtagaattta aactcgcctt cgccccacca ataacgttct aggcaggtaa
     1741 gcgcaaggta agtacccacg taatagcctt gaccctttaa tcgatcaatt aacttataat
     1801 cctcctgtaa tttggtaatg cgttcccgat gccaatttgc gtgaaaatat aggctatttt
     1861 cttccaattc gtccaccaaa gcgtaattta tcgtataaaa aaatgaggac atatccttcg
     1921 gatgtaaatt ctcgatcgta attttagcgg atgtttggaa aggcatttcg aaataggaat
     1981 taaagccccc tgtaggatta accactatcg gcaacgagtt cacctcgcaa cgctctccaa
     2041 acccattaca aaagaaatcc cctaaaggac tttcaacagc agggctatcc gacccatccc
     2101 aataaattct taagattaaa tcacgtaaaa caaaactgcc aaattctgtt cgatcacgaa
     2161 tcgtcatcca catatgacga attattcccg ttccagtaat atccgcaatc acagtttctt
     2221 caccagaagt cagcggaatt gcgccacgac ctttgcggct tggtcctagc tcgcttggca
     2281 acatcgcagc tttacctttc tcgcctgttg gattttcggg agtgattgct cgactctgga
     2341 tttttttaaa ttgggtaata ttttttagta tactcatctt tttctccttt gaatgtgaat
     2401 cgtttttccc ttttctacgt gaagaatttt tttcgtatca cttattttca atggcttttc
     2461 tgacaaattt gaaatactca actcggattg cgtacattca atcgaaatga aggcttgacc
     2521 gaacttcaaa ttcttccaag aaaacgttaa attatcaaca ggatcaattt gcaatactcc
     2581 ttttttgaat ttcaatccga aatattgact gatcattagg ctgacaaaaa gacccgatgc
     2641 ccagccgcat ttgccgcagc caaagtctcg cacgacttct ccttgttgag cctctaagcg
     2701 atacggccac caccaccacg aaccgtctaa atctgccaaa caaattagtt cttctaatcg
     2761 ggttacgaaa gcttgagggt tgtgcagctc gctcattaaa acagtgataa agccaggaaa
     2821 agtggcgccg gattctaatc cccaagtgat gcctttgatt tcctcgctat aggttggatt
     2881 gaaagaacta gcagaaaagg ccatggtgtt ctgatataaa tgatctttat tatctaaaaa
     2941 ttgataaaaa ggcatcaacg tagtgtctga ttcttctcca tcatgcatca ataaattaat
     3001 ttttccatcc acaatcacat cacttaaaaa gattaaccct tgctcaagaa tcggtttttg
     3061 atagtgcttc actgaatagg ttgttttctc ctcatcaccg attccctcca aaaattgctc
     3121 accaaaagga ccatcgatag tcatttgttt acaaattgat tcctttagtt tacaggcaat
     3181 gtttttgtat cgcttgctaa gactcagttg ttctagggct tcaaaaattt ctgcaagtcc
     3241 aatacaggcc ttccaaagac aaagattgct gccggtatga tactttccaa gtgcaaaggc
     3301 gtctgataac caaacagatg gaaaaagcat tggttcccct gaccgacgtt gagctaatat
     3361 gtcctcaata attcttgaag cattcagtag aacctgcgga caccgctcaa agaattttgt
     3421 atccccagta tgttcaaaat aaagactagc cagtaacgca ctggataacg aattactcag
     3481 agaatgattg atacctcctg taaattttga accaggaaac ttaacgccat attgatcaaa
     3541 ccatagaacc gttttttgac ataactccgg ttcaaacatg gtgaatggca atgatgcgta
     3601 atacatatcc ttattccaaa ttcgattagt agcaggatag ctgccccaat tgctgccgac
     3661 gacctgtccc tgtcgattca taccgaaact agaaaagctt tgataaagtg cacgacgata
     3721 gagatggaca actctatcgt ttggcattga cagctgccca aatagctgag caaaatagtt
     3781 caatgtttcc ttcagccatt tttcgatatc agattcctga aaagtcatta cttcttcaaa
     3841 agcatttggg tccacaaaag caatggcgaa gcttgccact tcctgttgtg cgatcgtgtc
     3901 cttatttcct ttttgagaaa tcaacacatt ttgctgatcc gaatacttct cttgatacga
     3961 aggcacatct ttcactgaaa cttgaaggct ttcgtgtgtt tcattcacaa tgtaaacctg
     4021 ttgaacaagc atacttaaac gcttattagc cagtatggga gcaaaggata ctacaatgac
     4081 cttaaacgtc gaataatgat gaatatatct aggcaaacag tcttgaatca catcgacttc
     4141 aatacgatga tccgattctg acaaatggta gacaatcccg tttacttcaa cctttaagga
     4201 taacgaattg gtttgagaga gctgtttatt gacccaaaca cctggttttt cctcagttag
     4261 taaattttct cgataaaagg tcaacgtttg aatcgtcccg caaacatcaa ggtttgctaa
     4321 tgtcttttga ttacttacat cgaaggtcaa tccctttaca tcaggaatat tccgatagta
     4381 gctgtattcg tttgaagtag tcacatttcc gtcgtttaac tcagcattaa aaaatgggtg
     4441 tttgggtgaa taaattactt cattaatagt catcttttcc ctccttgcta aaaaaactgc
     4501 agcctagtag ctgcagcttc ctactatgcg attccaagta atgacaataa aattcccaag
     4561 acgaccattc caaaaataat tttgatgacg ccgacttttt tatctagtaa tttgaaacag
     4621 ctcaatgtta ccaataacgg tacaattcct ttaaaaatac tatcgaggat ttcttggaca
     4681 ttcattagtt cttcgccctt cattgaaaag ctcaaaacgg tattgaattt gaccatactg
     4741 atcgtcattg cacctaccat gatcagaccc aagatactag cagcttttgt cagaatttca
     4801 attaacccgt tcgcatacag cttttcaata tatttagagc ctaatgaata gcctaaaacg
     4861 ccgccatagt atcgcatcaa gtaattaggt atattgaaca taactaaaaa gataattggt
     4921 gccaaaatat ttccggcatt ggctaagttg atcgctagtc ccgcggtaac gacacgccaa
     4981 actccccaga acaatgaatc gccaattcca gccaatggtc ccatcaaact cgttttgatc
     5041 gcgttgatac tttccacgtc aaaaccagct ttttcagaat tttccttctc catcgatgcc
     5101 gcaatcccca tgacaaagga cgcgcaagga atggtcgtcg aaaaatactg tgtatgacgt
     5161 tccagcgccg cagctcgatc ttcttcactt ttgtagaaat gattaatgaa gggcatcata
     5221 gaataggcga aaccaccgcc gcccatcgtc accggattca ccgaagcata catagtcatc
     5281 gagcgccaaa acatcttccg aaccaatttt ttctcatctt tagaaattcc atcagctaat
     5341 ttcattgaaa gaaatcctcc tcttcttggt caagcgttgc atttacagca gcatcgcttt
     5401 gaactgcatg aacagatata ttttttagtt gtagatctct ttgggctata acaattgcaa
     5461 taatcgttcc aactgcggca attgcaatca atggtaactc gagatacgcc ataacaacgg
     5521 ccccaaagaa aaaccacaca gcaatatttt tactccaaag catccgtaac agcatcgcca
     5581 tcccaactgc cggcagcaga tttccagcga ctgttaatcc gttcatgatg acctctggaa
     5641 tattatccaa gacgcttttg acaacatctg ctccaaataa aacggcaaag aatggaataa
     5701 gagcataaag cgcatagtgg acaaaccaca taaagaagtg cagattgatg attccttttt
     5761 gatcaccaga ttttgcaaat ttgaaaaatt tagcagccat cggtccacat acaaaagaaa
     5821 aaatcatatt cattaactgt aatcctaata ctgctagtgg aaagacaatg ggaataatcg
     5881 catccacgtt tcctcccatt ttgattgcga acgctgtagc cagcccagca gccaaagaag
     5941 gctccgccgt actagagatt ccgatattga cgacacccat aaagatggct tctagtgacg
     6001 ccccgacaat caaccccgtc tgcaaatccc ccatcaacgc tcctgctaac ggagcaacaa
     6061 caatcggacg actcagcatg gacatcccga acatttcatg tccagcaact ccaataaaaa
     6121 cggccaaagc gactacaaat gcatcataca taatttttct ccttcctttt tctaaaaatc
     6181 actcaaaata gagatagctg aacgtttttc catcgccggt gtaggctgaa aaacggtatc
     6241 tggataaagt tcaactaatt cttttaagtc ttgaatttct gacgtcgtta acatcacttc
     6301 ctttgataag gttttcttat cttccgatgg cggtgtcatt ttcccagcat tccccacgtt
     6361 cattaattcg atttccgttg ggaaagcttt cgcaactttt accgcatctt tgacattagg
     6421 gactaaaacc atcaagcgca tgtccttcgc ccgcggatct tttaacaaat gaatcgcgtc
     6481 atcaactgtt ttaataatga ctttgacatt cgatggtgca gccatcttca atgtcatctt
     6541 ttgaatatcg ttggtagccg cctcatcgct tgctactact aacccggtta aatttagact
     6601 ctttgtccaa gtcatcgcta cttgtccatg aattaatcgt tcatcaattc gcactgcttt
     6661 aatcattttt cttcctccta aaaaaaatct tcctcttcat ttactacatg tacctcatca
     6721 agaatggtca tctttaattc ctgttggcat ttatttacca gttcctgaat atcctccttt
     6781 gttaattcct tggttgccaa tactatttcg agcagaattg gcagattaaa gccagacaaa
     6841 atagttacag caatctcttt ctccaaaaag gttttggtaa tcttctgatt gacacttccg
     6901 ccaaataaat cggtaaaaac aaaaatctga tcctgctcgt tcacctggtt tacaaataca
     6961 ttcaactcct gttcaaaatc acactcatcc acataagcat taatcaccgt aatctcttga
     7021 accttccccg tcagtaattg aatactgcta agaaagcctg aagctaagtg accgtgagtc
     7081 gctattagaa ttttcttcat atggttaatt cctttctttc tgttcacttt tatataagca
     7141 attcctgtgc caacttttaa aaacattggt ttaatcacgt taattcacat taaaaaaagt
     7201 gtctaaccta aaaaaaacag attagacact tttttagaca ctttattttt gaatatacgg
     7261 tcctaataat tcatagagca gcgaaagctc ataagcattg actttaaaat tatatttcat
     7321 ctccattgat tgaaaaatac ttctagaaac ataataaaat tcatcttcat ctggtttcaa
     7381 atcataattc ggttcttcag atttttctgt ccgagccaag aataaacgtt ccatcatcaa
     7441 tgccgtatgc atatagagat ttagttttac acggccattt aaagtgaaat gatagtaatt
     7501 ttcatattta gaaatgactg tttccacttc actcatcacg atcgttgggt tcagaaagtt
     7561 caaacggtcc gcaattcctt ccaacgaaaa gaattttact agctcctgaa tcaagcggtt
     7621 aaattctgcc ggcttcattt caggactcaa aatatgctcc aaattggctg ctccatcacc
     7681 gtctaagatg tcataaacat taatattagg aattgaaaag gaacgtggaa gatccgaagt
     7741 cgtgatgacc aattttgtct tgtcaaaata agtttcatca ttttggtcga ttaattcacg
     7801 tagttctctg tagtccttcg tgaaaacttc gatggattca tccgataaat gatgacgaaa
     7861 aacatctttc aatttgtccg aaatccccaa acccgacata caggaaatga taatgttgct
     7921 actttgagaa aagccttcaa agtattgaac attgattgaa tacttgtctg ctgcatcctc
     7981 cgcaatctgt ttaaatggcg cattcatcgt catcttcaag ccaatatcaa gtgcaatgga
     8041 agtcgtcaaa ttattcagaa gaagtaactc cccttttaaa tcgttcttga tctgtgtgta
     8101 aatttgattt aaagagccca tatctacgat cagcactaga ctatctgtat tcacttgccg
     8161 tttcacgaaa ttccttgtcg attcgacaat ctctgacaca ttactctcta ctggcatatc
     8221 caacgcttca aaaacataat tgccgcataa ttgattcact actgcttgaa tgctgctagc
     8281 cgtactatct ccgtgagcaa gcaacaggcc cttcagctcg atggattcat ctacataatc
     8341 agataaagtc acgacaataa gcagtcttag aaagttctcg tccttttctg agagcggtgg
     8401 taaatgctcc aagaactgat tacataagta agtcgtccga ggtagatttg cagctaatgt
     8461 acgatttatc ccggttaagt ctgtcttata gcttggggaa atgacatagg atctgctaat
     8521 aatttctgca acaggtccac tcgtcgtcaa tccgtacgtt tttcctaaaa cggataccca
     8581 gacctttcta aacatctgaa tcatcaattt tctactcatt gataaaaaag aatccttatc
     8641 aatgtgatgc accaaatgct gtacttcatt ctttatcgaa gaaaaatttt cattgaaatt
     8701 accttcattt atcagctgag taattttttc caaaatcgaa tcataatcaa acggtttgat
     8761 tcgatccgtt tttatctttt gatttttctt tatcaataaa ctagcaatcg gcaagctttc
     8821 tacttcggat tccagcatat ttaactgaat caagatctca tttttcccaa tcgttaatgg
     8881 ttcttcttgt gttcgactat acacatccgc acaactgatt ttaatgatat tcttcagctt
     8941 accaatattc ccactaaagg gatgagataa cattaaagac aaggcttctc ttgaaacaat
     9001 aatatctttt tgtaaaacat ccgcttcatt ttgaaaaaat aattgaatca gctctaatcg
     9061 ttctcgaatg gggcgatctt caaaagttgg caacataaca ctaatttgaa tacgccgatc
     9121 aaaggtgtct aataaataat tttcaccttt ttccgtcgtg gcgaaaataa aacgaacctt
     9181 agcagaatgc caatttacat tttcaccgac tggacgatag tttccagtat caataaatag
     9241 aaagagcttt tcttgatttt ctttcgatag gcgatggacc tcatcgagaa aaagatagcc
     9301 gttgttggcc gttgccaata aaccatcggt atccttttca gcccccgtaa aggccccctt
     9361 cttgtatcca aacagtgttg ccgaaagcag ttcgggattg tccgcataat cggcacagtt
     9421 taagatagca aatggtgcat ctttttcaat aatttgctca tggatagcgt attcaaaaat
     9481 ttttgtggct aaaaaacttt ttccaacacc cgtggctcca tttattaata cactgagtcc
     9541 gttaggcgga tatttgacag cggctataca tttttgaatg acctctctca gacttccgtc
     9601 atgaccaact acagaatgaa aggcttcgtt tatttgctcc gtattatcta caaccgcata
     9661 ttcatctaaa ggcttccaca aaacaggctt tcccggtaat ttctcgacct ttttatcttc
     9721 cactaatctc gtcaaataat ggctggcatt ctgacgagag atacctagat tcttcgaaat
     9781 tttctcggca gtgacagaag acatattccc tgttagtcgt atcagctcat caaaaactcg
     9841 atctttagcg ctctttttca ttttttcctc cagtttaatg tgtctaaaag cataataaac
     9901 tatcggctgc aatatgtcta ttctatattt gtattccaat ttttactttg aaacacaaat
     9961 agtcgaatat ttgcctcaga tatctgacaa aataatttat ctaccaattt agtttttatc
    10021 taatatgatt tttatgtttt ttcatagcta caaatattga actggattca tatattagtt
    10081 ttattaatag attacagaag ggttaagtat atttgcatag tttccaatta ctttatcctt
    10141 ctgtaacttt ttcaatctta gatttactag cccgtctcgg gccctatttt acaatggttt
    10201 tgtccacttt tctctggtag aaaaacgtgg tagaaaaaat aaaataacac aaaaaaagcg
    10261 gcgtttcatc cgcttttggt agaagattgg aaaactcaga tagaaaaagg acgattttta
    10321 gctctttaga acgttgataa gtccccattc ttactgagtt ttgccattat ctcctataca
    10381 tcttgaattc taagaatagg tcattataga tgcctaggta tttcgatcct agatctttgt
    10441 agacttcaag atgggagatc gtgtcatcag atggatagaa ttgttcgtcc gacgaaatgt
    10501 cttttggcaa taacgctttt gcttcttcat ttggtgtaga ataaccaata tactctgcat
    10561 tttgtgccgc attttccggt tctagcataa agttgatgaa atcataggca ccttctttat
    10621 ttttcacggt ttttggcata acgatattat caaaccaaag attggatcct tctggtggga
    10681 tcacataatg taaatgttca ttttcccaca tcatatcggc agcttcgcca gaaaatgtca
    10741 ctgccacgga ggcttcttca ttgatcatat acattttgat ctcatctgcc acgattgctt
    10801 tgacatttgg tgttaaatga ttcaatttat ttgctgcttc tgtcagctgt gccatattct
    10861 tactattcaa agaatagcct aaactattca aagaaagccc cataacttct cgtgcaccgt
    10921 caatcagcat caaactgtct tttaattccg gcttccacaa atcatcccaa tgcttaattt
    10981 gatttgcatc aaagaattta tcgttatata cgatccctaa agttccccaa aaatatggaa
    11041 tcgaatactg attatctgga tcaaaatcca agtctaaaaa tcgtggatca atattattta
    11101 atcctttgat cttcgaatga tctaatggaa gtactagttt ttctttgatc attttttgga
    11161 tcatatattc acttggtata gcgatgtcgt agtttgtccc accttgctgg atttttgtga
    11221 acatcgcttc attagaatca aatgtttcat aattcacttt gtatccatat tctttttcaa
    11281 atttcgtgat caaactagga tcgatataat ctccccagtt atagatggta agtacctttg
    11341 ctccactcat gccacttgtt ttttcaagtt gacgaacacc aagcagcagc accgcaataa
    11401 tgatgagaat acctaaaaac agagattgta attttttcat cgcagccgtg ccaccccctc
    11461 gcgtactctt ctgtttttct tcagccgttt tggacggttt tctttactaa taaagtagta
    11521 tccaatgacc aatatcatgg agaacaagaa aactaacgca cttagcgcat tgatttctaa
    11581 actgattcct tgtcttgcac gagaatagat ctctactgag agcgtcgtaa agccatttcc
    11641 tgtgacgaaa aatgtaaccg caaaatcatc aagagaatag gtaaatgcca taaaatatcc
    11701 agctatgatt cctggagaaa gataaggcaa aatgatattt ttgactactt ggaaattatt
    11761 agcaccaagg tctctagccg catcgaccat tgaatcgttc atttcctgca attttggcaa
    11821 gaccatcaag acgacgattg gaatgctgaa agcaatatgg gaaagcaata cactctgaaa
    11881 acctaatccg aatcctagta gtgtaaagaa aatcaagaaa ctagcaccga taataacatc
    11941 aggagagact aataagatgt tattcatgct caacaacgct gtccgtgcac gtcgtttttt
    12001 cgtgtaatag attcccatag ccccaaatgt cccgatgatc gtggcgatca atgcagataa
    12061 gaaagccagc atgaacgtat tcaatacgat aatgatcaaa cgtgtatctt caaaaacagc
    12121 ttcataattt tcccaagtaa atcctgtaaa gctgttcatc gttcctcctt cattgaagga
    12181 ataaaaaatc agatagaaga tcggtgcata taagaataaa aagacgatcc ctaaataaag
    12241 atacgaccat ttaaattttt tcatttatga gcgacctcct tttttcttct cgcctgtcag
    12301 gagcatcacg atgaacatag caatgattaa aatcacaccg attgtagaac ccatccccca
    12361 gttttgtgta accaagaaat gctgttcgat agccgttcct aaagtgatca cccgatttcc
    12421 gccaatcaga cgagtcagca tgaataaaga aagagaagga ataaatactg cttggacacc
    12481 acttttcaca ccatttaatg ataacggaaa aatcactcgt cggaaggtct caaagctatt
    12541 cgcgcctaaa tctctactag cactgatcaa agaaggattc atctcttcca atgcattgaa
    12601 aattggcata atcataaatg gaatctcgat atacgttgcc acgaacagaa aactgaagtc
    12661 tgtgaataat atctgtttcg gcccgatccc gataaaatca aagaaatgat tcacgctgcc
    12721 gtgcacactg aaaatcccaa taaatgcgta agctttaagc agcagattca cccatgtagg
    12781 caaaataatc agcatcagcc ataattgctt gtgcttcagt tttgttaaaa aataagcagt
    12841 cgggtaactg atgatcagcg tgaaaaacgt gatcaaaaat gcataccata cagaattgat
    12901 cgtcatagaa agcgatgtcc ccgacgtaaa ataagataga taattattca gcgtgaattg
    12961 cccattcata tcaaaaaaag attgataaat gatcattaat acaggcgcga tcacgaataa
    13021 accgagccac aaaatataag ggacggaata gatcctgcgc attgttttca ttcttgtccc
    13081 ccctattctt cgtagctgtc aagacgtgca tcgaattctt cttcagactc gttgaaacgc
    13141 atgacatgga tatcttctgg ctcaaaggcc aatcctactt tgcttccttc tttagctttt
    13201 ttcgttgaat ggaccatcca ttcattttgc tgttcatcat aacagatgat ttcataatgg
    13261 acaccacgaa acagctgagt atcgaccgtt acgactagtt ttcctttatc tggtgtcgtg
    13321 atcgtcaaat cctctgggcg taaaacaact tcaactggtt catttagacg catcccgcca
    13381 tcgacacatt caaattgctt accaacaaac tcaactagat tatcttcgat cattacaccg
    13441 tccacgatat tgctctcccc aacaaaatca gcaacgaaat gattgattgg ctcatcataa
    13501 atatctacgg gtgtaccact ttggacgatt ttccctttat tcatgacaaa aatctcatca
    13561 ctcattgcca acgcttcttc ttggtcatga gtaacaaaaa taaacgtgat tcctaatcgt
    13621 tgttgcagat cccgcagttc atactgcata tctgtacgta gtttcaaatc aagcgccgat
    13681 aacggttcat ctaatagcaa tactttcggt tcatttacaa ttgctcgtgc aatcgctaca
    13741 cgctgtcttt gaccaccaga catttcacta atctcccgtt tttcgtatcc aggaagctgg
    13801 accatacgca aagcatcttt taccttcttt tcgatatctg atttggacat tttcttgatc
    13861 ttcaatccaa aagcaacgtt atcaaaaaca ttcatatgag gaaaaagtgc ataatcttgg
    13921 aagactgtat ttacttgtcg cttattagct ggcaaatcat tcaaccgttt cccttcaaaa
    13981 tagatgtccc cttctgtagc ttcggtgaat ccagctataa tacgtaggat cgttgtttta
    14041 ccacagccgg aaggaccaag cagtgtataa aattttcctt gttcgatctc aaaacttact
    14101 ttttttaata ctgtttcatc atcatagcgt ttcactactt gatcaaatga aatgattggt
    14161 ttccccacgt ttttaccccc tatattacaa atatgattgc gttgcaacaa tcaatacttt
    14221 tgttcgtttt ttgagatgat tccttaattg atgttgtttt gtcgcttgat aatagatcga
    14281 ctgtcctgct tttgccacat agaccgtctc tcctaagctt aactctaatt ccccttctaa
    14341 tacaaaaata aacgtttcag ataatgaagg ttcaaacgtc ttatattctc ctgcaccttc
    14401 aaaagtcagc aagacaggct ccatttcttt ttcattcgaa tcagccacga gccattccaa
    14461 ttcatacccg ttttcttcat cgaaataaac cgtatgatct tcttttgaat aaaccacttg
    14521 cagattcgct gcttcttgat gaaaaaattc ttctggagtc acacctaaga cttcaagaat
    14581 agaaaagaat gtttccattg atggtgaact taaatctcgt tccaactggg aaatataccc
    14641 ttttgacaaa tctgtgcgtt cgcctagttc ttcttgtgtc aaattctttt ggatgcgcaa
    14701 atttcttaac ttctcaccaa tttccataat ttcctccgaa tcgaagtttt ctaatagtaa
    14761 acttcctgtt tactaccact cgtattagta tacgaaattt attgaaaaag gcaacccttt
    14821 gtaaaatttt ttttgctgaa tatttcttta tcttttggaa taaattctag ttgtttgatt
    14881 ctttattttt cacctaattt ttgtctaaat ttacggaaga acaagaataa aagagtaggc
    14941 tttatggtat gataaaagaa aattttaaaa ggagtgtctc acgaatgaac gcttttcgat
    15001 cttttcaggt aataaaggaa cctgttttcc atacagcaat cagtgatctg ccatttactg
    15061 agttggctga aaatgaagtc ttggtaaaaa ttgcctattc tgatgtcaat tataaagatg
    15121 cattggcaat gagcgagtct ggtcaggtga tacgaacata tccgatgact ccagggattg
    15181 atttatccgg aactgtcgtc caaagtaaca atccacgttt ttcaaaagaa gatcttgtac
    15241 ttgctactgg ttttgggctt ggcgtcaccc atccaggcgg ttatagccaa tatcaaaaag
    15301 taccaggaga ctggttagtt cctctaccaa aaaatatgac gctgcgtcaa gcgatgatcc
    15361 ttggtacagc agggtttact gctatgttgt gtgtgaacgc tttaatccgt caaggtatga
    15421 cctccgataa aaaagttgtc gttactggtg cttctggcgg tgtaggcagt acagcgatag
    15481 ccctgttaca taaactagga ttccagtcaa tcattacctt ttccagaaaa gaagagtctg
    15541 tcacatggct aaaaagtcta ggtgctttgc aagtcgtacg acctgatgaa tttctgccag
    15601 agacaacaaa agctttagga aaacagcaaa tcgactatgt catcgacaca gtaggtggcg
    15661 agcagctaag cagtcttctt ccattgatct cttataacgg tgcagtagct ttatgtggca
    15721 atgctggggg aatcaaacta aacgcgactg tcttgccttt tattctgcga aacatccagt
    15781 tgattggaat tgattctgtc aatgtaccaa tagatcaacg tctttcttta tggcaacaga
    15841 tggcggattt gcagattgca gatgaactgg tcgtccaaga gattactctg gatcaattgc
    15901 ctgaaacagc atctaaacta cttgctggaa cacaccaagg aagaacacta gtaaatgtag
    15961 gtgaccataa atgaagattc taatcacagc tggaggaacg actgaaaaaa tcgatcaagt
    16021 acgtgctatt acaaatcatt cgacaggacg attagggcaa gcattagctg attatttagc
    16081 tgccaatccg gataccacaa tcgattacgt gactacgaaa caagcactga aaccgaaaag
    16141 acactccaat ataacgattt atacgatcga atctgcactg gacttatttt tacaattaga
    16201 agaactaacg aaaaaagaac actacgatgc gattatccac agtatggcag tcagtgattt
    16261 tactcctgct tttagttttt cagaagaaca gctggcaaaa aacctaccca cttcttcaac
    16321 gcaagaagaa ctggacaatt ggtttgcgga aaatgaacag acagatacca ctgtttcaaa
    16381 gatctcttct aacaccgagc atcttgtgct cgttttgaag aaaacaccaa aaatcatttc
    16441 ttatctacgt gaatggcagc caaaagcaaa aatcattggt ttcaaactat tagtagatgt
    16501 accaaaggaa tctcttctgg cagtcgcaaa aaatagtctg ataaataaca aaactgattt
    16561 tattttcgca aatgatttga cggaaatcca tggcgagaca caccacggct atctcttatc
    16621 taaagatgga acagtggaag aagctcaaag taaatctgag attgctgcat tgattactga
    16681 gaaaattaga ctggaggaat caaaatgaaa aagatcttac taggcgttac tggaagtatt
    16741 tctgcttaca aaagcgctga tcttacaagt caattcgtca aattaggtgc ccaggtagat
    16801 gtgattatgt ctaaaagcag cacacatttt atcacgccat taaccttgca atctctgtcg
    16861 aagcgcgcag tccatacaga cgtcatgcaa gaatcaaatc cgcaggtaat caatcatatc
    16921 gaactagcaa aacaagctga cttgtttctc atcgcgcctg ctacagcaaa cattatcggc
    16981 aagctagcaa acggcattgc agacgatctt ctttcaacag tcgcaatggc gcttccagtg
    17041 ggaacaccaa aattgattgc ccctgccatg aacacgaata tgtatcaaaa cccgatcact
    17101 cagcgtaatc tggaaacatt gagatctgtc ggctatcagg aaattgaacc aagagaatct
    17161 cttttagctt gtggagattt tggaaaaggt gccttagctg atattcagac gatcatcgaa
    17221 caggtcatgc tgactcttaa tgaaagaaat gcttagaaaa aaggagtact tatgaaaaat
    17281 acaaaaaact ttacattaac tgcgatgttt ttagctattt tgattttatt agctgttacg
    17341 cctttaggtt ttatccctat cggtccaatc aatgcaacaa ccatgcatat tcctgtaatc
    17401 attgcttcga tcgttttagg accaagacta ggtgcttttc ttggcggtac atttggactg
    17461 atcagcatga tccgtagcac agttatccaa acacctttgt cttttgtctt ttctccgttt
    17521 atcccagtca tcggaacaga acatggaagc tggaaagcct tgctgatcgc gtttattcct
    17581 cgcatcttga ttggggtggt tccttatttc gtttataaag gtctgcgcaa actaatgaaa
    17641 caaaaagcag attccgtttc tttattctta gctggacttt ccggttcatt gaccaatacg
    17701 attttagtga tgaatatgat ctacttttta ttccagcaag attatgccaa agtcatcgga
    17761 acaaatttaa atgctgttta ttcagctatt ctagcggtta tttttacaag tggtatacct
    17821 gaaggaatcg tcgcaggtct tgcgactgca gcagtatgca atgtcttgtt aagatacttc
    17881 aagcaccctt ctcttactaa ctgatagtca tcgtagccga tctctgattt ttttctcttt
    17941 tggtgttact attgaatgga aagatttgac gtcgaaagga tgatccaaat gaaaattttt
    18001 gttgtaggtg caaacggaca aattggccga cacctgataa aagatttagc tccaagttcc
    18061 catgaaattt ttgcaggggt gagagatgtt gcttcccaaa cattagtaaa aaaagagaat
    18121 gtttcgtatg tctcttttga tttaacttgg tccgtggaaa aaatgaccga agcattcaaa
    18181 ggcatcgatg tattgatttt tgctgccggc tctcaaggaa agaatcttct gcaagtagac
    18241 ttggatggcg caatcaaaac agtgatcgca gctgaaaatg cgcatgtctc tcgctatctg
    18301 atggtcagtg ctgtttatgc agatgaacca gcaaaatggc ctgagtccat gacagattat
    18361 tatattacta aacattatgc agatgaatgg ctgaaacgaa ctaacttaga ttttgtcatt
    18421 cttcaaccag tcacattgac taacgatgaa gaagttactt ccattcaatt aactaagcct
    18481 aatgagaagg catcaaaaac gatcactcgc tctacagttg ctgctgtact tgccgctcta
    18541 gtggaagaaa cggatatttc tcgtacgact ctagtacttt ctgaaggtag caaagagttg
    18601 aatacagcat tccaagaatg ggcaaaggag gaatagatcc atgcgtgcca tttcaatcaa
    18661 gcatcctggt tctgtagatg tattagaaca aattgaagtg cctgttccta aaccaaaaca
    18721 aggcgaatta ctgatcaaag tccatacagc agctgtcaat cgaacagata ttatgaaaag
    18781 agaatcagtc tctttgaaac ctccctaccc tattttggga gtcgaagtag ctggagaggt
    18841 agttgagaat aaaagtgata tctccacttt tactcctggg acaaaagtat acggattagt
    18901 caatctaggt ggttatgccg aatatgctgt gatgcctgcc gatcgtgcaa tccttctccc
    18961 tgatacgcta gactatgttt ctgctgctgg aatctcagaa gtttttttga ctgcttatca
    19021 aacactttac tggcttggaa aattacaaga aggcgaaact gtcttgatcc atgcaggagc
    19081 aagtggtgtg ggaacagcag cgatccaatt agctagacag ctttctcacg cgaaaatcat
    19141 cgttactgca ggatcagaag aaaaactcgc cttttgtaaa gaacttggtg cagatgaatt
    19201 gatcaactat aaaaaacaag actttcaaga agaggtacga aaaatcactg aaggtcgggg
    19261 agtcgatgtt attttggatt tcatcggcgc ttcttactgg gaaaagaatt tagcttctat
    19321 agccgttgat ggacgttggg tcttgattgg tatgctaggt gggaccatcg tgcctgagat
    19381 agaccttggt gtattgattg ggaaacgtgt ccaactgctt ggtacattat tgacacctag
    19441 aagtgatcag tataaagcag agctcgcaca aacttttatg caaaaagctg ggccttttct
    19501 agaaaatggc aagattcggc caatcattga ccgtacattc caattcagcg cagtcaaaca
    19561 agcgcatgaa tatatggaag caaacaaaaa catcggaaaa atcattttga agataactga
    19621 ataaaatcat gctgatcaaa aaagaggctg ggacagaagc gtttaactcc aagaaataag
    19681 aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt tcagcttatt tccgaaggaa
    19741 tttcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt
    19801 ttgtcccacg ctctttt
//
LOCUS       DAZKNM010000041        18779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000041 DAZKNM010000000
VERSION     DAZKNM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.041"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8M9_001138"
     CDS             <1..435
                     /locus_tag="ACG8M9_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0377925.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8M9_001139"
     tRNA            complement(759..842)
                     /locus_tag="ACG8M9_001139"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8M9_001140"
     CDS             1370..1870
                     /locus_tag="ACG8M9_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0377926.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8M9_001141"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8M9_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0377927.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG8M9_001142"
     CDS             complement(2558..3478)
                     /locus_tag="ACG8M9_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0377928.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG8M9_001143"
     CDS             3665..4156
                     /locus_tag="ACG8M9_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0377929.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG8M9_001144"
     CDS             complement(4180..6555)
                     /locus_tag="ACG8M9_001144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0377930.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG8M9_001145"
     CDS             6881..7507
                     /locus_tag="ACG8M9_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0377931.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG8M9_001146"
     CDS             7530..8234
                     /locus_tag="ACG8M9_001146"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0377932.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACG8M9_001147"
     CDS             8426..9259
                     /locus_tag="ACG8M9_001147"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0377933.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="ACG8M9_001148"
     CDS             9456..11384
                     /locus_tag="ACG8M9_001148"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0377934.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="ACG8M9_001149"
     CDS             11522..11977
                     /locus_tag="ACG8M9_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0377935.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12057..12185
                     /locus_tag="ACG8M9_001150"
     CDS             12057..12185
                     /locus_tag="ACG8M9_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377936.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            12447..12716
                     /locus_tag="ACG8M9_001151"
     CDS             12447..12716
                     /locus_tag="ACG8M9_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377937.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /gene="rpsT"
                     /locus_tag="ACG8M9_001152"
     CDS             13116..13370
                     /gene="rpsT"
                     /locus_tag="ACG8M9_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0377938.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="ACG8M9_001153"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="ACG8M9_001153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0377939.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="ACG8M9_001154"
     CDS             complement(14543..16816)
                     /locus_tag="ACG8M9_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0377940.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="ACG8M9_001155"
     CDS             complement(16809..17348)
                     /locus_tag="ACG8M9_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0377941.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="ACG8M9_001156"
     CDS             complement(17484..17702)
                     /locus_tag="ACG8M9_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0377942.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="ACG8M9_001157"
     CDS             complement(17699..17944)
                     /locus_tag="ACG8M9_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0377943.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..>18779)
                     /locus_tag="ACG8M9_001158"
     CDS             complement(18060..>18779)
                     /locus_tag="ACG8M9_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0377944.1"
                     /translation="VMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFCIAS
                     GFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGSVKE
                     PGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEHLDQ
                     LASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKVEDL
                     QEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5770 a         3637 c         3304 g         6068 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaaca aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattac
//
LOCUS       DAZKNM010000042        18726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000042 DAZKNM010000000
VERSION     DAZKNM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.042"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..1805
                     /locus_tag="ACG8M9_001159"
     CDS             120..1805
                     /locus_tag="ACG8M9_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0377945.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1819..3102
                     /locus_tag="ACG8M9_001160"
     CDS             1819..3102
                     /locus_tag="ACG8M9_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0377946.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3172..4041
                     /locus_tag="ACG8M9_001161"
     CDS             3172..4041
                     /locus_tag="ACG8M9_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377947.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4038..4874
                     /locus_tag="ACG8M9_001162"
     CDS             4038..4874
                     /locus_tag="ACG8M9_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0377948.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4900..5967
                     /locus_tag="ACG8M9_001163"
     CDS             4900..5967
                     /locus_tag="ACG8M9_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0377949.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5964..6791
                     /locus_tag="ACG8M9_001164"
     CDS             5964..6791
                     /locus_tag="ACG8M9_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0377950.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6836..7861
                     /locus_tag="ACG8M9_001165"
     CDS             6836..7861
                     /locus_tag="ACG8M9_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0377951.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7858..10041
                     /locus_tag="ACG8M9_001166"
     CDS             7858..10041
                     /locus_tag="ACG8M9_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0377952.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10041..11171
                     /locus_tag="ACG8M9_001167"
     CDS             10041..11171
                     /locus_tag="ACG8M9_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0377953.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11168..11845
                     /gene="pgmB"
                     /locus_tag="ACG8M9_001168"
     CDS             11168..11845
                     /gene="pgmB"
                     /locus_tag="ACG8M9_001168"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0377954.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11929..12456
                     /gene="msrA"
                     /locus_tag="ACG8M9_001169"
     CDS             11929..12456
                     /gene="msrA"
                     /locus_tag="ACG8M9_001169"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0377955.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12657..13343)
                     /locus_tag="ACG8M9_001170"
     CDS             complement(12657..13343)
                     /locus_tag="ACG8M9_001170"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0377956.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13423..14961
                     /locus_tag="ACG8M9_001171"
     CDS             13423..14961
                     /locus_tag="ACG8M9_001171"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0377957.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15081..15548
                     /locus_tag="ACG8M9_001172"
     CDS             15081..15548
                     /locus_tag="ACG8M9_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0377958.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16082..16510
                     /gene="rplM"
                     /locus_tag="ACG8M9_001173"
     CDS             16082..16510
                     /gene="rplM"
                     /locus_tag="ACG8M9_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0377959.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16526..16918
                     /gene="rpsI"
                     /locus_tag="ACG8M9_001174"
     CDS             16526..16918
                     /gene="rpsI"
                     /locus_tag="ACG8M9_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0377960.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(17006..18232)
                     /locus_tag="ACG8M9_001175"
     CDS             complement(17006..18232)
                     /locus_tag="ACG8M9_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0377961.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(18304..18594)
                     /locus_tag="ACG8M9_001176"
     CDS             complement(18304..18594)
                     /locus_tag="ACG8M9_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0377962.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
BASE COUNT         6058 a         3411 c         3949 g         5308 t
ORIGIN      
        1 tttcttattt gtatttttgt gtatatcaaa attatttaaa aaaatgctat tgacagtaag
       61 cgttttccat aatatgatca aatcgttggg aacgctccca aaaaagaaag gggaatccga
      121 tgtatacaaa acttttacaa gcgatttatc gcgactcaga tttgtctgag aaaaccgcct
      181 ctatgattga acaaaaagtc agtgtttatc agcagaagaa aggccaggca ggcaaactgc
      241 attgggacga agaagatatt ttcttgatta cttatgggga tcaatttttt gaagagcagc
      301 aaccaacttt gcgtacattt aagaaatttt atcaaaagta tttggcagat acatttagag
      361 ttgtacattt cttgccattt ttcccatata catctgatga tggtttctcc gttgtaaatt
      421 atcaggcaat caacgaccgt ttgggcacat gggaagatat agaagaaatg agaagcgaaa
      481 cacggttaat gttcgacttt gtctgcaatc atatgtctgc cgaaagtact tggtttcagg
      541 aatatttaaa cttgaatgat acgtacaaag atttttttat tgatttgcca ctggatacag
      601 atacaagcag cgtgactaga ccaaggacca gcccgttact tacaacgttc attgacaaaa
      661 acgggaaaga aaaacacgta tggacgactt ttagcgaaga tcaagtagac ttgaatttta
      721 aaaatccaga agttttattg aaaatgatcg atattctttt gttttactta gaaaaaggcg
      781 cttcatttat tcgcttggac gctgtgggat ttttatggaa agaactagga acttcttgta
      841 tacacctgcc agaaacgcat aaaatcatcc aattattccg tgcaatcgct gatgaagcag
      901 ctccaggaac ggtattgatc actgagacaa atgtgccaca caaagataat atcagttatt
      961 ttgggaatgg cgagaatgaa gcacagatgg tctatcagtt ttcgctacca cctttgaccc
     1021 ttcaagcgat tcgttcgggt aatacaagta agttgaaaga atggttaaaa accatcgatt
     1081 tcgcatcgaa aaaaacaact ttttttaatt ttttagcctc ccatgacggt atcggattga
     1141 atccggtacg aggtattctg ccggaaaaaa cgatccttga attagtggaa cagctgaaga
     1201 aagagggtac ccgagtaaac tacaagaaaa acccagatgg aacgaccagt ccatatgaga
     1261 taaacgctac gtatctagat gcgttaagtt ctccaacaga tacgacggaa tggagaagca
     1321 aacgcttttt agctgctcat tcatttttgg taacgttacc aggcgtgccg gcaatttata
     1381 ttcaaagtgt gctgggaagt cgaaatgatt acaaaggcgt agaaaaaaca ggagagaatc
     1441 gaaaaatcaa tcggcaaaaa tttttgttag cagatattga gaaagagttg cagacagatc
     1501 cattaagaac agcagtatat ggtgaattga cccagatgct atctattcga aaaaaagaaa
     1561 aagccttcca tccgaatagt ttgatttact tactggactc agcagattct tttatcagtt
     1621 ttattcgcga agcgaaggaa gagaaagtct tagttatcca taatttttcc aatcaatcag
     1681 caggttatct tttaccaaaa ggagattacc tagacctgtt ttctggacaa attatccatt
     1741 gcagtcaaga acgaaagttg atattccagc cgtatgaata tatgtggctg aaaataataa
     1801 aataagaggt ggaaaaaaat gaaaaaaatt attgttacta cctgtctgct agctactgga
     1861 tttcttctta ctgcttgtgg tgaaagtcaa agctctaacg aatcacaaca gacaacgatt
     1921 caattcatgc actcttctgt cgaacaggaa cgattaagtg tgatcaatga actggttgct
     1981 gattttgaaa aagaaaatcc agacatcaaa atcaagcaag tgccggtaga agaatcagcg
     2041 ttcaatacaa aaatcgttac gttagctcgt tccggtaaat taccagaagt aatggaagtc
     2101 agtcaggatt ttgctaaagt gatggataaa gatgaactga ttgatcagac ggcagtccaa
     2161 aacgttatcg aggaagctgg agaagataat tattatgacg gagccaaaaa ccttgttcgt
     2221 tcagaagatg gaaaaagcta tattgctgca ccaattagcg gctgggttca aggtatttgg
     2281 tatgacaaag aaaaattatc agaagctggt ttttcagaac cagaaaattg ggatgatatt
     2341 ttaaaaattg ctcaacattt cacagataaa gagaataaac aatatggaat tgctatgcct
     2401 actgctgaag gaacaatgag tgagcaagcg ttctctcaat ttgctttgtc aaatggagca
     2461 aatgttttgg atgatgaagg cgaggtaacg atcgatacgg aaaagatgag agaagcattg
     2521 tcgttttatc aaaacttatc ccaatacacg atgccaggct ctaatgatgt gacagaaatc
     2581 aaagatgctt tcatgaatgg aacagcacca atggcaatct attcaactta cattttgcct
     2641 tctgtttatg aagcggggaa ctcagaaaat atcggttttg ctatcccaac acaaaaagat
     2701 caagctgtct atggcacagt atctggattg acgatttctt ctggtctaga cgagaaacaa
     2761 aaagaggctg ctgaagcatt cacagctttc ttatctcaac cgaaaaacat ggaaaagtgg
     2821 gtcttgatgt ctcctggtgg tgctcaacca gtgaataaac aagtagtcga actggatggt
     2881 tacaaagaaa atgaagtcat caagtctttt ggcgagctgc catctgagat tgctagtgct
     2941 tttgatgaag tacaagtctt tggactagtc ggggaaaaga atttcacgaa aatgggagat
     3001 atcacaagct caggtgtgat cggaaaagca gtgaaccaag taactgtcgg aaacgaagat
     3061 gtcgatcaag ctttagctga cgcacaaaaa aatacgaaat aacaagaagt tctgccaatg
     3121 gtgttcgaaa aaagtagata gctattggca gattatcagg gggaggtttc aatgaatcgt
     3181 ccaaggaaac attctgactt aaaattagcc atgggacttt tagcaccaag catgctttta
     3241 ttagctgttt tagtgctgat tccaatgctt tccaatattc agatcagttt tttcgatatg
     3301 ccgctgaatc ctaactcaga cgccgttttt atcggattaa gcaattatct tgaaatttta
     3361 agtgatagtg aattttatcg ttctttaggt ttaacagttg cttacacatt cttagttgta
     3421 gcaggcagta cagctttagg attagccgta gctattttgc ttaaccgcaa gttcagattt
     3481 aggaaaacgg tgcgttcact gattttatta agttatgtta cgccatctat ttctttgatt
     3541 tttgcttgga aatatatgtt caataatagt tatggaatcg tcaatgatct gttagtagat
     3601 aggttgcagc ttttcaacga agcaccgctg tggttcgacc agccagttgc tagcttcttt
     3661 atggtcgttt tgtttgctat ctggagatac tatccgtatg cgtttatttc atttttggct
     3721 attttgcaaa cagtcgacaa aacgctttat gaagcagctg aaatggacgg tgcaaatgct
     3781 tggcagaagt tcaagatcgt gacactgccg gcaatcaaac cagtactagc aactgtgatt
     3841 gttttacgat ctatctggat gttctatatg tttacagatg tctatctact gacgaataaa
     3901 gtcaatatct taggcgttta tttgtatcag acagcctttg cgttcaatga tcttggaaaa
     3961 gcagcagcta tatcgattct attgttcatc attattgcag cggtcgtctt gctctcacga
     4021 aagaaggtgg acttgaaatg aaaaataaaa agagcaggaa aatcttattt tacgcgacga
     4081 ttttgttgct attagctgtc aatttgtttc catttttgat catgctgatg acatcattca
     4141 aggattcaca agaagcaatt gcaacgacac aaactatttt tccgaaagaa tggacttttg
     4201 aacattatca ggatattttc aatgctgaga tttttccatt catcatgtat ttcaaaaata
     4261 gtttagtgat ctccttgata gcctccgtaa ctgcagtagt gattgggatc agcggggcat
     4321 acgctttgtc aaaactacgc ttcaaagctc gctcgaaaat caatgctagc ttttacaccg
     4381 tttatatgtt ttccggtatt ttgatcgttg ttccattatt taaaatcatt tccggtctag
     4441 gtttgtatga taccaataca gcactgatta tcacgatggt cgttcaaaca cttcctactg
     4501 cgatattcat gctaaaaagt tattttgaca cgattccgac tgatattgaa gaagcagcaa
     4561 tgatcgatgg tcttagccgg atccagatca ttattcggat cattatacca ttggctattt
     4621 ctggaatcat ttctgtcttc gtttattgct ttatggtttc gtggaatgat tatttatttg
     4681 cttcgatttt cctgtcgacc tccgaaaaat ttactttacc aatcggtttg aacacattat
     4741 tcagtacacc agactacatt tggggacgaa tgatggccgc ttcattggtg accgctttac
     4801 cagtcatcat tatgtatgcg ttatctgaac gatttattaa aggtgattta gtcgaaggcg
     4861 gcgtaaaagg ataaaaagaa aagaaagagg aaagaaaaga tgaaaaaatt agttgcatta
     4921 gaaccaagaa tagccggatt caaagattac gaagaccgtg agatcgaaaa gaacgaagta
     4981 cgtatccgag taattttcgc tactccaaaa ccaggaacag aaattgtcga ctttagagga
     5041 ttaagtccat ttatcgatga agaattttca gaagaatggc gcctattctt gccaagagaa
     5101 gaaggaagcc aacgcggtgt ggtatttggc gaatttcaat taggaaatat gattgtcggt
     5161 acgatcgaag aaacgggcag tttggtgaca gagtatcaaa aaggagagtt agtctgcact
     5221 tatggaccca ttcgtgaaac gctgattaca aacggtgttg ataattataa actacgtaaa
     5281 cttgatcggt tggatcaatg gaaaaatgca gtatgttacg atcctgctca attcgcttta
     5341 ggtggtatcc gggaagcaca tgttcgagca ggtgatgctg tagcaatctt cggtttaggc
     5401 gctatcgggc aaattgcgat tcaattagct aaaaaagcag gtgctactac catcgttggg
     5461 atcgatccca tagcgcagcg tagagaatta gcagaaaaat acggtgccga ttacacgcta
     5521 gatcctatga aagcagatgt cggttttcgt ctaaaggaat tgacagataa acgtggagta
     5581 gattcagtta ttgaaacgag tggcagtgct gccgcattac aagcttcttt aagagggatc
     5641 ggctatggcg ggatcatttc ttatgttgcc tttgctaaac cttttccagc tgggtttaat
     5701 cttggtcgcg aagcacattt caataatgca aaaatcgtct ttgctcgagc atctagtgaa
     5761 ccaaacccag attacccaag atggaaccgt aaacgaatcg aggacacatg ttgggaatta
     5821 ttgatgaacg gttacttgga ttgtgaagaa atcattgatc cagttgtgcc atttgcagaa
     5881 agcgcagaag cttacatgcg ttatgtagac cagcacccag aagaaagtat caaaatgggc
     5941 gttcaagtcc gaggaggaga acaatgaaat tcggtacgca agatcaagca tttttcccaa
     6001 gagacatttt agaaaaattt agatttatca aagactgtgg atttgaagaa tacgaaatcg
     6061 acggtaagtt gttaatagaa aatatcgaca cagtcaaaca ggcaatcaaa gaaacaggga
     6121 tcccagtagt gacagcttgt aacggctacg atgggtggat cggtgacttt atcgaagaac
     6181 ggcgtcttaa tggtgtacgt caaattgctg agattttgcg tgcactaagc gaggttggag
     6241 gcaaaggaat cgttatccca gcagcttggg gaatgttcac ttatcgatta ccgccgatgg
     6301 tttcaccccg aagcaaagaa ggagactata aagcagtcag cgaatcactt cgttatttag
     6361 ataaagtagc cgaagaaaca ggtacggtta tttatttgga gcctttgaac cgataccaag
     6421 accatatgat caatttgtta gcggatgcgc gtcattacat tgactctaat caattgaaac
     6481 atgtgcagat catcgctgat ttttatcata tgaatattga agaagatgat atttcaaaag
     6541 cattgactac gcaaaaagag tatgtgggtc atatccattt agcagataat catcgctacc
     6601 aaccaggatc tggcgcaatt gattttactc gccattttca aacgctactt caaaatgagt
     6661 atgaaggtta tatggtatat gaatgtcgtt tgcgaggaga aaatgaagtg aaaatttatc
     6721 aggaatcatt gcaatacttg aaaaactgtc tggaaacagc gcgtcattta aatgatttag
     6781 agaaagaata gagaaagaat aacaataagt aaataagcaa aggagggaga aataaatgaa
     6841 gaaagtagca atcataggcg ctggacaagt agcagaaaaa gtacatgctg cttattatca
     6901 aaaaagatca gatgtcttag aattggccgc tgtggttgat ccagtcagcc agcgagcaga
     6961 agacttttgc cgacgtacag gattttctca tgcgtataca tcagttacac aaatgctgaa
     7021 tgagatttct cccgatatcg tgagtgtctg tacacccaat cgctttcact tcgaacatgt
     7081 attggctgcc ctcgaagcta atgcttcagt gatgtgcgag aaaccaccag ctatgacagc
     7141 tgatcaagca agaaagatgg cagagacagc cgataaaaac gggcagattt tggcttatgg
     7201 attccagcat cgtttttctg acgaagcaac aatgatcaag aatcatcttg atgttctcgg
     7261 ggatatttat tacgtagaag cgaatgcgct tcgtcggtct ggtgttccag gctggggaac
     7321 gtttatcgac aaagaacttc aaggaggcgg gcctttgatc gattatggca ttcacatgct
     7381 tgatacagcg atgtatatta ctggatttcc taaagtaaaa agcgttcggg cgtacagctt
     7441 ccaaaaaatt ggtacgaaaa aaaattcagg gaaattcggg aaatgggatc caagcaaatt
     7501 cacagtagaa gatgcaattt ttggcacact ggaattagaa aacggcaaga tcatccaact
     7561 gaatacttcg tttgctttga atatcagaga agacaaactg aatgtggctt tttgtggcga
     7621 tcaggcaggt gctaatctgt atccaggcga actattcgaa gatgtagatg gaaagctggt
     7681 ttccttacaa aagaccacgc cagttaatga cccacatgaa ctaagtatgg ctcattttgt
     7741 gaatcgggcg ctcggtgacc agactcagat gattgcagat ggctggcaag gatatgtcat
     7801 ccagaatctg gtggaaaatt tatatcgttc ggcagaaaca ggagaagtta ttaccttatg
     7861 aaagtagaag aaaaacagtt tgatttagag tatatcaata agtatgcgag tcagatgacc
     7921 attggaaatg gattttttgg tatcagagga agccaggagg aagactatcg gaatcaagta
     7981 aggggaatgt ttgcagctgg tgtatataac cgacctttag gagcagaatc agccgagtta
     8041 gtgaatctcc cagatgtcat cagatgggaa ataaaaatcg acggtgaaca tttttctctt
     8101 gagacgatga aagttcttgc atacgagcgg gcactagatt tagaaatggg agagcttaaa
     8161 cgctatgcgc tcattgaaac tgctcaagga aaaaaagtgg aagtcattac ttgtcgtgtt
     8221 gctgatcaaa ataatctcca tcaggcagcc ctgaaagtgg tcatcaagcc gttgaccgaa
     8281 gactgccaag tagatgtgaa aacgggtatc gatggacagc aaaccaattt tggtacacag
     8341 cagttggttg aaaaagaact gcgagtattt ggtgacgatt tgatcagtgc atcctaccag
     8401 acaacagaaa gtaaaattga tattgggatt gcagctaaat tcaataaagc aggtgtattc
     8461 caagcaaaaa atcgccggat caccgcttcc ttttcagaaa agatcagaaa aaatacgtcc
     8521 tttacgctgg agaagatcat cgtgctctct acctctcttc agcaaacaga cccgtttaag
     8581 aaaagtattg cccaagccca gaaaagcgtt agttatacag aaattttaga gcagtcgatg
     8641 tcttattgga cagatttttg gcatcagcat cggataacga tccaaggcga ttcttctttc
     8701 gatcagttag cgatggactt cgctgcttac catttacaga ttatgactcc ccgcaatgat
     8761 ccaagtgtta gtgtaggggc gaagggacta actggcgaag gttataaagg gcatgttttc
     8821 tgggatacgg aaatttttat cctgcctttc tttttatatc agtttcctga agtagcaaag
     8881 aatttgctgc tgtaccgtta tgatcgatta gaaggcgcac ggcaaaaagc aagagattat
     8941 cactatcaag gtgcactttt cccatgggaa agcgcacaca ctggtcagga agagacaccc
     9001 aaatttgctg cattgaatat caaaaccgga acaagacaag aagttgcttc agctgccgca
     9061 gaacatcata tcagtgcaga ccttgcttat gccgttcaag agtatgtcca agcaacagag
     9121 gatcaatcgt ttttagaaac aaagggaaaa acgctcattc atgaaacagc agctttttgg
     9181 ctgagcagag ctgtaaaagt cgatgatcat ttagagatac acagagtcat tggtccagat
     9241 gaatacacag aatatatcga caataatgca tatacgaact atctggcgca ttataatgtg
     9301 gccttagctg ttaaatatca agctggcgat ccgctttttt tgaaagaatg tcaaaatttt
     9361 ttagataagt tatatttgcc agtgcctaac aaagaacagc tgattccgca agatgacacg
     9421 tatctcagta agccagttat tgatttgacc acttacaaag aacgtcctgg cagccagtct
     9481 attttacttg attatgcccg tagcgaagta aatgaactcc aagtcagcaa gcaagcagat
     9541 cttgtcatgc tgttatactt attacctgag tatttttctc aaccaatcgt ttctaagaat
     9601 ttgcattact atgaaaacaa aacaatccac gactcatcgt tgagtaaagc gattcacgcc
     9661 atagaggctt gccgtatcca agaaatggaa tgggcgtatc atttattcca agaagcgtgc
     9721 ctgattgatt taggaccaga tccgcattca tcagatgacg gaattcatgc tgcttcatta
     9781 ggagcactat ggctagcagt tatatttggt ttttcaggta ttacagtaac gaatggaact
     9841 ctttctattt ctccaagact tccttcagca tgggaaaaaa tttcttttcc ttttatttgg
     9901 aaaaatcaca ggttgctatt tgttttgaca aaaaaacaaa tagtaataaa aaaagagacg
     9961 aaagaaacat tacctatttt tgtcaatgga gaccaatacc agctggaaaa agaactgatc
    10021 ttggaacgga ggggaaaata atgcgtattc tgattgcgcc agattctttc aaagaaagtc
    10081 tgacagcctt agaggcagca caagccattc aatcaggttt tcaagaagtt tttgttcagg
    10141 ctgaatatga tctgctacct ataggcgatg gaggagaagg aacggtttca tcattagcag
    10201 acagtttaag gttgaagcgg caaacagcag tcgttactaa tgcatttggc aaaaacaaag
    10261 aaatccagta cgcttcagac ggtcaaactg cgatttttga aatggcagaa atcgtcggat
    10321 tgaaagatgt cccactaaac caacaacagc cacttaggtt atccacgcga ggagtaggag
    10381 aactgatacg acaattgttg caacaaggtg ttcgacacat catcatcggc gttggcggaa
    10441 gtagtaccaa cgatggcggc atcggcatgg cagaaggtct aggttatgct ttttacgatc
    10501 aagataatca aaaagtagaa gcactgggag aaaatctagg taaaattgct acagttgata
    10561 aaacgaacgt gcctattgag ctgattaaag ccaaaatcac tgtcattact gatgttacta
    10621 atcttttatg cggaaaagaa ggcgcttctt ttgttttcgg accacaaaaa ggcttgaaag
    10681 aaaatgaatt agcagtggct gaccaagcca tgcatgattt ttatatgaga tttgcccctc
    10741 aagtaatcga ggcatcgggc agtagtgctg gtggtggaat gggtgcagga ctgttagcat
    10801 ttgcttcagg gaaaatagtc tctggcatcg actacgtatt ggatttactg gagttcgata
    10861 aacgggtaca gcaagcagac ctcgtaatcg taggcgaagg acgtctagat ggacaaagtc
    10921 taaaaggaaa agcgccagtt gggattgcta gaagagtacc agagggcgtt cctgtgatag
    10981 ctatttgcgg aagtgttggt aaaggaagcg aaaaagcagc agatgttggc atttcggccg
    11041 tttttccgat ccttcctgca gctgtgcctt tacaagaagc tttacagcaa gccactgata
    11101 atcttcatcg cacagctaaa aacatcgcat ctttacttca agtcgttcat tttcaaggag
    11161 gtaatcgatg aaaatcaaag cagcactatt cgatttagat ggcgttcttg tagacacagc
    11221 tcgttatcat tacgaagcgt ggctggtttt agctaaccag ctgtccattc cattcactga
    11281 gaaagaaaac gagcaactga aaggaatatc cagaaccgag tcactagagc gattgctgtc
    11341 atttggaaaa atggaacaga aattctcaga aaaagagaaa tcagcctttg ctgagcaaaa
    11401 aaacaatcta tatcttcaag cgattcaaaa aatggatgaa acatccgttt tgcctggtgc
    11461 aatagctgtt cttgagtact tgaaaaaaac aaacatcaag atcggattgg gatcagcaag
    11521 caaaaatgca cgcctcattt tagaaaaaac gaatttgaca agttattttg atgtccttat
    11581 tgacggcacg caagtatcaa aagcaaaacc tgatccagaa gtttttctca aaggagcaca
    11641 gcaattgaat gtaccgccaa atgcatgctt ggtcattgaa gattcagaag ctggttgtca
    11701 agcagctttg gctggtaaca tgcatgtatt gggaataggt gaaaacataa atcttccttc
    11761 tgctgaatat gtgattcctg atttaaccgt ttttgatcaa gtacgttcgt tttggcattt
    11821 atcagaagcc gtatcacgta tataatagtt tccgtttttt ctgtcacaag caaaacgtta
    11881 tcctcctttt ggaaatcaag gtatgatgag aaaaaagggg gattttatat gacaaaaaaa
    11941 gcagttttcg caggcggatg cttttggtgt atggtcaaac catttgaaga gcagccaggg
    12001 atcatttctg tgacatccgg ttatactggc gggcatgtac caaatccaac ttatgaacaa
    12061 gtaacaacag gcacaacagg gcatacagaa gcagtagaga tcgaatatga tccagaagtt
    12121 atttcttatg atcagttggt ggaaatctat tggcagcaaa ccgatccgac tgacgcatta
    12181 ggacagttcg ctgatcgcgg tgattcttat cgtccagtga tctactataa tgatgaagaa
    12241 gaacgaaata ttgcagagcg ttcaaaacaa gcattgcaag acagcgggcg attcgataag
    12301 ccaatcgtga caaaaatcga accaagaagc gaattttatc ctgcagaaga ttaccatcag
    12361 gattattaca agaaaaacaa gctccattac aatctttatc gagaaggttc tggacgtgca
    12421 ggtttccttc gtcgacattg gggcgtcaag aaataagaag ccgaaaaagc tacttatgga
    12481 acaccgtata tcagaggttg taaaaccagc attttgagct aatactgagg gtcagctgat
    12541 tgaacaccat ttagtggaga ggttgtgaac ctgtcgccta gctccaagtc acaaggattt
    12601 ggtaagagaa cactatatat cagaggttgt gacaagtcgt ttgtcacaac cttttttcaa
    12661 tacctaagat gaaggacaat cagcaacgca ctgctcaaaa aaggaatgaa aggcagcttt
    12721 tccaactttt ttttatgaca gataaagtaa acagaaaaaa cggatattcc aaacagacta
    12781 gcaagaaaca agatctgaag caactcttct agagacaagc caccactcca agaaagcatc
    12841 aacagagtat cgccagctcc gagataggat tgaccatagc gaaggcacac accgcctacg
    12901 ataaacgtta acagcacact ttgccattgg aagacgccgg cctgcaacca aaaaatccat
    12961 aaaattgccc aagaacagaa tagcgtgtga gaatggacca tgaagtaaaa taaatccatc
    13021 aacgaaaata gcaaagccga aagcatccat atgaacgtcg aaaaatttct tgcggaagaa
    13081 aaagtaaaga accaggcaaa aagaccacca cataaggctt ctgccaacaa atagtagaag
    13141 ggcaaccgac acctgcaata gcggcagcga aatcgttgga cgatgatcga gagaatcgga
    13201 atcaattcat accaagaaag cgaacgacag catttgacac aatgagatct aggataaata
    13261 atagagtgac ctaacggaat ccgctgggcg gttaaacata aaaatgatcc gatacagcaa
    13321 ccaatgataa aataaagtaa cacgccatct cccccttttc aaaaccaaga tacgcaagct
    13381 taaactaaat ttttgtacat acataaaatt tgaggtgaaa aaatgaacgt ttcaatcgga
    13441 ttagatatcg gaaccacaca aaccaaagca gttgctttca cagatgaagc ccaagaagca
    13501 gcatccgctt attttcgcta ccctttgatc caagaaacac aagggatggc agaacaagat
    13561 caggaattga tctttcaagg tgtctgcacc gttataaaag aagtaattaa acagttgcct
    13621 tcagacacaa tcattcaatt tgtctccttc tcctctgcga tgcatggcct gatattgttg
    13681 gatgaagaga aaacaccgtt atctcgaatg atcacttggg cagataaccg agcagcggac
    13741 gaagcagaaa aactgaaata taccgtgctt ggcaaagaat tttatcggca gactgggacg
    13801 ccgattcatc caatgacacc attgaacaaa atcaagtggc tgcaaaacag ccaacctgaa
    13861 ttattcaaaa aaactgctta ttttgtcggt atcaaagact atatctttta tagattgttt
    13921 ggcgaactga tcagcgacta cggaacagct tcaggaactg gatattttga tatccatgaa
    13981 ttccaatggg cagaagaagt gcttgactac ttgaacattc gaatagagca attgccgcaa
    14041 gtctttcctt ccacctatca agtaacaggt ctaccatcaa aaacagcagc agaactaggt
    14101 ttgccaaatg atatatcatt tgttttggga ggggcagacg ggccgctttc taatctaggg
    14161 ttaggtgctt ttggtgatac gatcgccgct ttgaccgtag gaacgagcgg agcacttcgt
    14221 tttattacta ctcagcctca gttccatcca gaaatggaga cgttttgtta tgttctggat
    14281 cagaaacatt gggtagtggg aggagcgact agcaatggtg ctggcatctt tgattgggca
    14341 gggcatacat ttttacagga agtcgttcaa tctgctaaag aaaaaggaga aaatccatat
    14401 gatgcactgc tttcggctat cgcttttgtg ccagcgggtg ccaatggtct gatttttcat
    14461 ccctatttac ttggtgagcg agcaccgctt tgggacgcag atgcgtttgg cagttttatc
    14521 ggtttacagc ggcaacatac agaaaaagaa atgatgaagg cgattctaga agggatctgt
    14581 ctcaaccttt atcggatttt aaaaggagtt tgccaggaaa atcagctcac agagatccgt
    14641 gctacaggcg ggtttgctca atctccgatt tttcgtcaga tgatggctga tgtactgggt
    14701 tacccgcttg ttttccctac tgtcacagaa gcatccgcat taggagctgt gattttaggc
    14761 tggagaagca tcggaaagat ccaaagtatc agtgaagcac aggaattgat agaattgaaa
    14821 gagcaagttg gagtacaaga aactgatcat aaaaaatatc agaaaatcta tccattattt
    14881 gtttcgacac aaaagcattt agcacagact tatcagcaat ttgctcaact gcgagaagaa
    14941 ttagctgatt ctgaaaaatg attggcttta cggataaaaa agcgaactga aaatcgttct
    15001 caggtaaatg attttgttgt acttttcatt gaaagtctac tattatttta agtgtaagaa
    15061 aatgttagga ggcaagaaag atgaaattcc aacaaaccaa agaagtatta aatcaattag
    15121 ttgcagatct aagccaaatg tctgttgttg ttcaccaaac acattggtac atgagaggac
    15181 caggtttcct tacattgcac ccaatgatgg atgagttcat ggatgatttg aacgctcagt
    15241 tagatgaagt atccgaacgc ttgatcacat tagacggttc accttattct acattgcgtg
    15301 aatttgcaga taacacaaaa atccctgatg aaattggccg ctgggatcgt actattccag
    15361 aacgtttgga aacacttgtt gcaggctatc gttatctagc agatctttac caaaaaggaa
    15421 tcgaagtttc tggagaagaa ggcgacgatc caacacaaga tatctttatc gaattcaaaa
    15481 cagccacaga aaaacgtatc tggatgatcc aagctcattt gggcaaagcg ccagagatcg
    15541 acgcgtaaaa actgttgttg aacttgaccg aaaaagaggt tgtgaaatca gcgttttgac
    15601 ctgagtatta gagaaacaaa cgtcaaaata gttcctcata ttttgccgtt ttttgagcta
    15661 ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtaagcct gccgttcagc
    15721 tccgagtcac aagaaacaat tttaaaaaac agcttcttat gttttatgga tattgtgact
    15781 tgtgaccgag gagttggcag gagaacaccg tttattcaaa ataaggggtt gtgacaagac
    15841 ttttgtcaca gcctcttttt gtttttcatt ttgacaggaa acgacccttt tatataaaag
    15901 aaagaaaaga agtaaagttt catataaatt tttcaatcaa gcagaaaaac gttgacattg
    15961 actagattag cgggtaatat gttaaatggt attgtttgcc ccacaaagag ccccggaaac
    16021 tcgaagtgta tgtcaaacat cagaacttaa attggaggaa aataacgtgc gtacaacata
    16081 tatggccaaa ccaggcgaag tagaacgtaa atggtatgta gtagacgcaa ctgatgttcc
    16141 attgggacgt ctttcaacag ttgttgcatc tgtactacgt ggtaaaaata aaccaacttt
    16201 cacacctcat ttggatactg gcgatttcgt catcgtcatc aatgcagaca aagtgaaatt
    16261 aacaggtaaa aaagcgactg acaaaattta ttaccgtcac agcaactacc caggaggatt
    16321 gaaatcaatc actgctggcg aattgcgtgc taaaaattct cgtcgtttga tcgaaacttc
    16381 tgtaaaaggt atgcttccta aaaacacttt aggccgcaaa caatttacaa aattgaacgt
    16441 atacggtgga gcagaacatc cacatgcagc acaacaacca gaagttttag acatcacaaa
    16501 cttaatctaa taggagggaa tttcattggc acaagttcaa tatatcggca caggccgtcg
    16561 taaaaacgca gttgctcgcg tacgtctagt accaggaact ggcaaaatta ctgtaaataa
    16621 aaaagatgtt gaagaataca ttccacatgc tgacttgcgt gaagtaatca atcaaccttt
    16681 cgctgttact gaaacaaaag gcgcatatga cgtatttgtt aacgtaaacg gcggaggata
    16741 cgctggacaa tcaggagcta tccgtcatgg tatcgctcgt gcattgctag aagttgatcc
    16801 tgacttccgt ttagctctta aacgtgctgg cttacttact cgtgacgcac gtatggtcga
    16861 acgtaaaaaa ccaggtctta agaaagcacg taaagcttca caattctcaa aacgttaata
    16921 tcctttatat atcaacgttt ctggcacttt tcaattattt ggaaagtgct tcttttgttt
    16981 ttaagaacac atttaagaac acatatcaat cacacttatt caatcctctc tgaaaagctt
    17041 caaaaacagc cttatcaact atatctggtg tgttcacata tatctttgtt gtagaaatat
    17101 ctgagtgtgt taaagcattt gaaatttgat tcatatctgc tccaccttgt ctagctatag
    17161 ttgcataagt atgacggagt ttgtgtggag ataaatgaac taagtcagga tgccttcttt
    17221 taattgaatt gattcgatag tttaggtaat cggcatgaac aggacaattt acttctccag
    17281 atggtttagt ataagtaaag agatactgat ctaacgtttg cttaattttt aatttcaaca
    17341 attgatcagc ttgtaccgat ttccattcac ttagcaatgt cattacaact tctggtactt
    17401 gaatgacggt atcttttcga ccttttgtaa acttttttct ttgatattta tctaaagtat
    17461 gtttaagacg gaccgtttgt ttttcaaaat caatatattt ccattgcaaa gcgtaagtct
    17521 cactttttct atcaccaaga tacagggtaa tcataaaaag tgtgtaatca tgaaaagtaa
    17581 gtaaatgatt attaaaatca tcatttactg cttctatcca attagtaagt tcttttgatg
    17641 atagtgcttg cttaccttct tgtattcttt tttcttctaa agcatttttt ttaggtgcag
    17701 tgatactttg aataatttta gtagttcgat taaattcgat ataatttagt atttctgcaa
    17761 tatcgaacat ggaacgaaca tatcctttta tgatttttat atttgcatat tctttcgatt
    17821 ttttggttaa ttcatctgat attatctcag tattgatatt tagataattc attgcgtatt
    17881 caccaaacat aggtaaaata tgcaaccgaa atatatcttt tgtattacta atggttatat
    17941 cactaggagg cctgtttgat ctaattgttt gacctaattc atatctagga agccatttag
    18001 attcgtaaaa ttttttaaac gttatttttc cttttaattc taatgagttt gcattctcct
    18061 ctctcaaaac tttttctatt ttcttcttca tatcattttc ggctttaatc gccaattgcc
    18121 tatttggaaa tgttttttga tacttttctg atgcaattcc tgttatttct ttaaaccttt
    18181 ttggatatct cagattagtt ttatatttcc catttgaaag ctttgtaggc aaaatcctca
    18241 ctccttaaaa aaagtaaggc agtatatttc actaatttta gtatatgcgg cgagaagaaa
    18301 atttcaagta ttcataaagt ctttaatatc ttctacgtca aagcgaacta caccaccaac
    18361 tcttattact gggaaattat atatacgaat ccacttatct agagtattat tatcgattcc
    18421 tagaaatgca caggtttgtt ttttattcag gtagactctg gattctataa aatcatctcc
    18481 taaaatagta gttatgaaat ttttgaattg ctttgggtaa gcacatagga atttcacctc
    18541 tccccagttt acggcgagcc ttacggaagt atcattattt gctgctattg tcatggcatt
    18601 ttgttttccc agaacaaaaa gaaatctcta agtatttgtc gctaatcagg tcatggcgta
    18661 cttgagtttc tagctcgaat agcagggatc gttattcaaa aattgtattt aattgtcaat
    18721 gatcaa
//
LOCUS       DAZKNM010000043        18712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000043 DAZKNM010000000
VERSION     DAZKNM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.043"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..715
                     /locus_tag="ACG8M9_001177"
     CDS             56..715
                     /locus_tag="ACG8M9_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0377963.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            800..1705
                     /locus_tag="ACG8M9_001178"
     CDS             800..1705
                     /locus_tag="ACG8M9_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0377964.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1707..2339)
                     /locus_tag="ACG8M9_001179"
     CDS             complement(1707..2339)
                     /locus_tag="ACG8M9_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0377965.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2490..2615
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2659..3183
                     /gene="infC"
                     /locus_tag="ACG8M9_001180"
     CDS             2659..3183
                     /gene="infC"
                     /locus_tag="ACG8M9_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0377966.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3255..3455
                     /gene="rpmI"
                     /locus_tag="ACG8M9_001181"
     CDS             3255..3455
                     /gene="rpmI"
                     /locus_tag="ACG8M9_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0377967.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3508..3867
                     /gene="rplT"
                     /locus_tag="ACG8M9_001182"
     CDS             3508..3867
                     /gene="rplT"
                     /locus_tag="ACG8M9_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0377968.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4119..5456
                     /locus_tag="ACG8M9_001183"
     CDS             4119..5456
                     /locus_tag="ACG8M9_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0377969.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5504..7633
                     /locus_tag="ACG8M9_001184"
     CDS             5504..7633
                     /locus_tag="ACG8M9_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0377970.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            7608..8297
                     /locus_tag="ACG8M9_001185"
     CDS             7608..8297
                     /locus_tag="ACG8M9_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377971.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(8705..9391)
                     /locus_tag="ACG8M9_001186"
     CDS             complement(8705..9391)
                     /locus_tag="ACG8M9_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0377972.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(9527..9721)
                     /locus_tag="ACG8M9_001187"
     CDS             complement(9527..9721)
                     /locus_tag="ACG8M9_001187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0377973.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(9771..10211)
                     /locus_tag="ACG8M9_001188"
     CDS             complement(9771..10211)
                     /locus_tag="ACG8M9_001188"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0377974.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(10215..11060)
                     /locus_tag="ACG8M9_001189"
     CDS             complement(10215..11060)
                     /locus_tag="ACG8M9_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0377975.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11209..12627
                     /gene="pepV"
                     /locus_tag="ACG8M9_001190"
     CDS             11209..12627
                     /gene="pepV"
                     /locus_tag="ACG8M9_001190"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0377976.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(12684..13631)
                     /locus_tag="ACG8M9_001191"
     CDS             complement(12684..13631)
                     /locus_tag="ACG8M9_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0377977.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(13854..14204)
                     /locus_tag="ACG8M9_001192"
     CDS             complement(13854..14204)
                     /locus_tag="ACG8M9_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0377978.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            14404..15483
                     /gene="pepA"
                     /locus_tag="ACG8M9_001193"
     CDS             14404..15483
                     /gene="pepA"
                     /locus_tag="ACG8M9_001193"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0377979.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            15627..15947
                     /locus_tag="ACG8M9_001194"
     CDS             15627..15947
                     /locus_tag="ACG8M9_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0377980.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            15969..16433
                     /locus_tag="ACG8M9_001195"
     CDS             15969..16433
                     /locus_tag="ACG8M9_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0377981.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            16638..17243
                     /gene="ytpR"
                     /locus_tag="ACG8M9_001196"
     CDS             16638..17243
                     /gene="ytpR"
                     /locus_tag="ACG8M9_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0377982.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(17282..18571)
                     /locus_tag="ACG8M9_001197"
     CDS             complement(17282..18571)
                     /locus_tag="ACG8M9_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0377983.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         5877 a         3470 c         3852 g         5513 t
ORIGIN      
        1 tttaaaaagg ttggagttaa acacaatatt tagtgatgaa gtgagctgga ggtaaatgag
       61 cgtgatcgtt ttagcaggta caattggtgc cggaaaatca agtttaacag aaatgatggc
      121 tgaacacttt gatagtcagg cattttatga atcgattgat gataatgaag tcttgccctt
      181 attttatgca aaccctgaac agtatgcttt tttgctgcaa atttattttt tgaataaacg
      241 ttttgcaagc atcaaacaag caatgaaaga tgacaataat gtattggacc gttcgattta
      301 tgaagattca ctgcttttcc atctaaatgc agatctagga agagcaacag aaacagaagt
      361 tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa cttccctatg ctgcccataa
      421 aaaacatccg gatttattag ttcatattcg tgtatctttt gatacgatgt tggagcgtat
      481 cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat acgattatta
      541 caaagaattg aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat
      601 ccaaatcgat ggagaccgct ataattttgt ggaagatcct caagcaaaag aagaagtctt
      661 gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc aaagtagcca tttgatttac
      721 agttgctcaa ataaagggta aacctttatt tgagcgactt ttttctttat actggaaaca
      781 aatgtcagga ggatagagta tgcgggaaaa acactatttt aatcaagacc gtaatttttg
      841 gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag aatatggcat
      901 tagtgacgag atgctgactt attctctaga taaaaatgaa cgggcgcgcg tagagtacga
      961 ttcttttgat gaagcgttac tgcttgtatc aaatgttccg caccagcaaa aagtcgataa
     1021 tcattatgaa actagtccta tcgcttttat tttaaaagaa gacggtttgt ttacatttac
     1081 cacgccgaat accgagtacg tgattcgact aattcgatcg cttttggaac gaatgccaga
     1141 catgagtgta tacagtttat tgtttcgtac attgtttttg atttctgatt ccttttttcc
     1201 tttaatcgag gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac
     1261 aacgaacaag aatctgctgc aactttcaga tttagagatc ggtttggttt acttagttac
     1321 aggaacaaaa caaaatgtag tacttttaga acaaatcaag gcattggcta tttatcgcaa
     1381 gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg atcgaagcac gacaagcagt
     1441 tgaaatgacg aacttagctt cgcagatttt agaccagctc tctggcactt ataataattt
     1501 gctgaataac aacttgaatg acacgatgaa atttttgacg gtctggtcgc tgatcttgac
     1561 cgttccaact attgtgacag gattcttcgg aatgaatctc cagttaccct ttacccattc
     1621 cgttttcgga tggggaatcg ccttgatcat cagtttggtc ttatctattt ggatgcttat
     1681 tgcactgtgg cggcggattc gttgatctag tctttgatct gcaaaccaag ggcttgggcg
     1741 atcacaagtg cttgctcttg gttgacgatt agtcccgcca aagaatcttg agataaagcg
     1801 attgctgtga agaaattagt cgtaaagtct aatcccttca gagaagttcg aaaccagttc
     1861 gaaccggtca actgattgtc agacaaaaaa aggtttttcc aagtgacctc ataaaattcg
     1921 ctgtcttcta attgacaatg atcgaaggag acagctttca agtttgtact actaaaggaa
     1981 gccatcgaag caatacaatc agtgaaagta cagtctcgaa gataactttc ggcaaaattc
     2041 gtacctgtta atttacattg atgaaacacg acttggtgga aactgccacc aatccactcc
     2101 agattggaaa aatcacattt ttcaaaaatg acattactgc attcaaaatg acgtaaaact
     2161 gttttttgca tcgtaagttt ttggatatga ctctctctta ataccatatt tttggcatcg
     2221 agatagcttt ggtcaccccc gttgatcaga agaccagaga agatctcctc atcttctaaa
     2281 taaaactctc cctttgtaag aagaggaaga gaaggtggaa taggttcgat ttttttcatg
     2341 tgggttcacc tcgtttttct ttagtatagc aaagaagcgg gaatcactgc aagaaagtct
     2401 gctaatctaa tcattttcag aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga
     2461 ttagtttttg ctatactatg atggttgaga aataaaagca gaagcacccg cttctcgcct
     2521 tagttgataa tgtcactggg ctagatagtt ttcactggtt ctattaggtg aagtacgcgc
     2581 gggttctgta agagaactcg tttttttatt gccgtttatt cggtaagctt ttctttctct
     2641 cataatattt ggaggtgaat gaccatagca aaggatatga tggttaacga cggcattcgt
     2701 gcacgagagt tacgattgat cggttcagat ggtgagcaat taggagttaa gacaaaagca
     2761 gaagcattga atattgcaga acaagcaaac ttggatcttg tgttagttgc ccctggtgcg
     2821 aaaccaccag ttgcgcgaat catggactac ggtaaatacc gtttcgaaca acaaaagaaa
     2881 gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct
     2941 acgatcgact tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag
     3001 ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg ccattaccca taaagaaatt
     3061 ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg atattgcaac agtggaacaa
     3121 aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag
     3181 taatttgaat gactaactgg ctgttggaca gccgatagtc ggataacgaa agattttgag
     3241 gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac
     3301 gtactggtaa aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca
     3361 aaacaaaaaa acaacgccgt caattgcgta aagcaagcat ggtttctagt ggcgacttca
     3421 aacgtattcg ccaacaacta gcaaaaatga aataagaagt acaaataaca gaatctgaag
     3481 atttattttt attaggagga attatacatg gcacgtgtta aaggtggaac agtaactcgt
     3541 aaacgtcgta aaagagtgct aaaattagca aaaggttact acggttcaaa acacacttta
     3601 tttaaaacag caaaagaaca agtaatgaat tcatacaact acgcatatcg cgatcgtcgt
     3661 caaaagaaac gcgacttccg taaattgtgg attgctcgta tcaacgcagc agctcgtatg
     3721 aatggcttga gctactcaaa attaatgcac ggtttgaaag ttgctgaaat cgacatcaac
     3781 cgtaaaatgt tagctgactt ggctgttaac gatgcagcag catttactgc attggctgac
     3841 caagcaaaag aagctttggc taaataagct aaacaattat aaagaggaaa gaagataacg
     3901 aaaagttatg ttctttcctc tttttttatt tacaatcctc catcaaatga aacaggacaa
     3961 taacgtataa gtctaaaata gcataattaa ggttttttaa ttgaaaactg aacatttatg
     4021 gtcacccttt aatgaaagcg ctaactacat aagttagaat gagtttgact gatatattag
     4081 tttgcttatc agaaaaacaa aatggacgga ggaataacat gagtgatatt attattggta
     4141 ttttgttagt tatctcattc tttttcatgg tctggtactg tgtaaaaggc tacaacttga
     4201 tggtcggctt tgccatcatg gctactgtat ggatgggatt agcgctagtc ggaaacacgt
     4261 tttcaccaaa cccggcgatg gaagggcaag gagtaattga tgtgttgacg catatctata
     4321 cgacggggcc agctgagtac gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt
     4381 tcgggcgtgt tctagtagat agcggaatcg cagctaccct gattcgaaag gtagttgaac
     4441 ttggagggga caagccaaga atcacgatga gcttactatg tattgtaacc gctgttatat
     4501 tcatgtcgat gacaggaatc ggaccagtga tttctattgc agttattgtg ttgccaatct
     4561 tgatgtcatt aggtatttca gtaccggttg cactgttctc atttatgggc tcgattatgg
     4621 ctggaatctt tgctaatatc gtcaatttca aacaatatca aacgatttat gctggtttca
     4681 atccagcggc tgaaagctat acttataatg actatttcca aattggtatg attgggatga
     4741 ttgtaagctt agttgtggta ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct
     4801 atgcaatggc agcaaacgta ccagctgaag gtggcgacgc accaatgatc tcatggttag
     4861 ccgtattatt accagtttta ggggtcgttc ttttagatct tccaatcatc ttaggtttta
     4921 ttctagcagg tatctgggca ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa
     4981 tctgtcgtca gtttgcgaaa ttatttacag atggcgcagt agatgttgcg ccaatggttg
     5041 gtttcttgat gaccttagcg atgttcaata attcagctgc gtatgcttct ccttattttt
     5101 cagctatttt tggagattgg ataccgcaat caccattagt cttggcaatc gtatttgcta
     5161 tattgacacc actaggattt ttccgcggtc caatgaatct agtcggttca ggctcagcta
     5221 ttttagctgt cgtattagct gttaatccaa cgatgtcgcc agcctttttg ttcccgttat
     5281 ttgcaatcac aacgattgct cctcagcatt tagatattac acaatcttgg gttgcttggg
     5341 gattaggtta tacaaaagta acttctcgag aatatatgaa gaaatccatc ccaactggtt
     5401 ggatcatcgg agcgatctta tgcctgatca cgttcttatt gtacgggaac gcctagtaaa
     5461 caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg
     5521 aatgggaatt gatgttggcg gtacacatac gaaagcagtc gcaatcgata atgcgacgca
     5581 cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat gatgtccgtg gagtagccgc
     5641 aggtgtcgtt cagtcttttc aaaattgttt gctggaaaat aatatcagtc cagaagatgt
     5701 tgtgtttgtt gcacattcta ccacacaagc gactaatgca ctgattgaag gagacgtagc
     5761 aaaagtcggc gtaatcggga tggcaaaagg tggattagaa ggttttttag caaaacgaca
     5821 aactagattg aacgatatcg atttagggaa taagaaaaaa atagagattg tcaatgcatt
     5881 cttacctgtc aaacacttaa atgtagaccg tgtttcagaa acgatttctt ctttagaaag
     5941 agaaagagcg gaggtcttgg tttcttctat ggcatttgga gtagataatg gggaaccaga
     6001 acgagttgtt tatgaagcag catcggtaaa atcgatccct acaacgatgg cttctgacat
     6061 cacaaaatta tatggattga caagaagaac tcggacagca gcaatcaatg catctatttt
     6121 accaaaaatg ctagatacgg caacctctac agaagattcc gtacgagaag caggggtcaa
     6181 tgtttctttg atgattatgc gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa
     6241 acgacctgtt ttaaccatgc tttccggtcc agcagcatca gtcatgggct ctttgatgta
     6301 cttgagagcg tctaatggtg tttattttga agtaggcgga acaacaacca atatcggggt
     6361 cattaaaaat ggacgtccag caatcgatta ttcaattgta ggtggccacc caacatatat
     6421 ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt tcgatggtac gggctaatca
     6481 gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt gccggtctag attatgcagt
     6541 atttacggag acagaaaaaa tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg
     6601 agaccctgct gattacgtca aggtagtgat ggaagacgga gaagaagtga ctattacaaa
     6661 tacatgtgca gccaatgtcc ttggattagt gcaagaagaa catttttctt atggaaatgt
     6721 cccatctgca cgaaaagcca tccaagcatt agctgattat tgtcatacga cggttgaaga
     6781 catcgcagaa cagatcatgg aaaaatccta tgctaagatt gaaccggtga ttttagaatt
     6841 ggcagacaag tatcacttgg agaaagatca gatttctctt gtaggtgtag ggggcggcgc
     6901 tgcctcattg attacttact ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa
     6961 cgccgaagtg atttcttcta ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg
     7021 tatcatccct tcgccaagta aagaagatat acgatcgttg aaaaatgaag cgatgaataa
     7081 agcgattgaa tcaggcgcta caccagaatc gatcgaagtc cacgtagaaa tcgatccaca
     7141 gacttcaaaa gtaacggcta tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac
     7201 caaggagatc acaacagaag aagcactaga gcttgctgca gaggatatgc gattaaataa
     7261 aaatgaagtt tgcttattgg aaaatacacc tttcttttat gtatgcggcg aacagaatcg
     7321 ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa ggatttatca aggtccaacg
     7381 tggacatgct tcatgtatga aaacaactgc agccaattat atgacagctg tcgaacaact
     7441 ttgggaagat atggcagttt atcaaactga attgattgcc cgaccagaat tttatctctg
     7501 cttaggagca agagtctctg attttacagc gacggatctg gaacaactac agcttttgat
     7561 ggatctggaa gtatctacta tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat
     7621 caaacaaaca taagttagaa gagttactaa acgaattaca acagatcccc gaagaaattt
     7681 ggggattcta ccagtttcgg cgagatttat tttggaaaaa gattcctttg tccaagcaga
     7741 aaattcttat acaacaatca atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa
     7801 agtacccttt tgccgatgta ggagaaattt gtgaacaaat ggctgttcca atcgtttcct
     7861 gtgaatcaga gcaaataaac gaacgaatca cttttgcaac ttatgcagaa gatgaaggga
     7921 ttcgattgat gacggagccg ttagaaaaat taaaatgttc aggactagct tcgatctcta
     7981 aagaaacagc gcaagctttg attattggac atgaactatt tcaccacatc gaagcttctg
     8041 ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac
     8101 atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat gagtttttca aaagagatga
     8161 atcaaagcga tttttcgcct tatgtattag aggcagtctt actttggcca tataatgaaa
     8221 ttcgtagcca agggatctta gaagaaataa aaaaaataga aaaaagatgt acggagtatg
     8281 atttagatta caaataaaga gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa
     8341 taaacgatgt tctcctgtca actcctcggt cacaagtcaa aatattcaac aaacatgaga
     8401 agctgttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     8461 ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacata
     8521 agaagctatt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     8581 ctcaataaac ggtgtgcaat cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat
     8641 atgaggaact attttgccgt ttctttctct aatactcagg tcaaaacgct gattccaaaa
     8701 cctcttattt gatgggtctg tcctgaatat cttcaaaata atcggggtaa ttatgaatca
     8761 cgtattcttg ctcctcgata atcatatgta aatgcatcgt gcaaagaaat ccgacttcat
     8821 caaagttatg atcaatcatt gattgaagca ttgtctgatg ttcatctaat actcgacccc
     8881 agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg gttcaagtgc gtactataac
     8941 tttctagcca atcccaaatt tctcgttttc cagcaatttc ataacaagtt cggtgaaaat
     9001 cagtatccaa atcaaaaaaa gcttttttat tttgagcgat tactgcctga tcggtttgtt
     9061 ctagtatttt ttctagttta gcttgatctt gcttggtcat tttcgcactg cattcttgca
     9121 ggatcggttt ttccaacttt tcccgcgtat aacgggctaa tgaagcagat cggatatcga
     9181 tttttgaaac ataagtgccg gactgtggaa tggtttttac aagctcttga ttgcgcagtt
     9241 ggatcagtgc ttcttgaatc ggtgtacgcc cgatagctag ttgttcttct aacactttat
     9301 cggagatttt acttccaggt gctaactcgg aataaatgat ttgtttacgt atagattcat
     9361 aagcttgctg ttgcaaatta ttgataacca tttaaccttc ctttctatgc agatataatc
     9421 tattataaca taaatcgctt acaaatcgtg tgggcataaa aacacagtct gcaaaaaaag
     9481 ccctgatagg catgcctatt agagcctcga aaagaaaaat attgaatcaa tctggtcgta
     9541 cctcttggat cggtttgaca cgaccgccga ttcttttagc aaaagcttcg gattcttttt
     9601 tgtctttgaa aatgagcaga ttgtctaagt ttttttcccg agtgcgacag ccagttttat
     9661 cgatgtaacc gtgcattact ttaacgacat acatggggat cacctgcttt tttcttttca
     9721 ttatacgaac tactcgacac aatacaagaa aaatgcttag acggtcttta ttaagaattt
     9781 acatcatcca aagaagcgta cgcttggaat aactgatcta actccggatt gactttgctc
     9841 agcttcacta atcgttgcga atcagaaagt aaaaaacagt gtttactact gccagtagtt
     9901 tttaattttt tgtcttcagt tcccacaatt tcgtagctaa aatccaaccg tgtccccgta
     9961 tacttttgga tagttggatg gatgctcact ttttctccgt aatggatcat ttctttatag
    10021 ctgcaattta cttccagaac ggggatgatg atcccagatt cttcgatttt tttataggga
    10081 aaccctagat gtaccaataa ggctacccga gcctcttcaa accagcggat ataattgctg
    10141 tgatgaataa tccccatttg atctgtttca taataaaaag gttctcgcac ataacctgta
    10201 aatttctcca tgactcatgc ctcgttttcc attgattttt tacaaaaata attcatccat
    10261 ttcggaagtg cttcgctgat ttgtgtcggc aaaacaatat attgttcttt tgccagctgg
    10321 tctccaatga aactatgaag ataaatagct gctgcaatcg tttggtcatt ttgaggaaac
    10381 tgggctaaaa atcctgtgat catcccagct aaagtgtcac ccatgccgcc cgttgccata
    10441 gcagcagaac ctaaaggatt ggtgtagaag tgctggtgac cataaatttt tgtctcatga
    10501 cttttcaaaa cgagcacagc accgatttcc tgttgcttct ttttgttttc tttatcccgt
    10561 tgttcttgta tcggtagaga actcaaccgt tgccactcca tttgatgagg tgtaagtaca
    10621 gtttgttcgg gatagggtag caaaagatga ttagcagcaa gtaaacttaa agcagagcca
    10681 tcaatcacca gccattgttc ttttgattgg ttttctagta cttgtttgaa acgttccaaa
    10741 ctcttagaag aagtacccaa tccgggacca atcaagagta catctgcttg ctttattacg
    10801 gaaagaaact ggtcagtatc cgtccaaact acaatcattg cttctggcag atgactgtga
    10861 agagcactcc gatttttttc atcagttact acagtcgtga gacccgctcc gctgtgtacg
    10921 gcagctaaag cactcataat gattgctccg ccatattgtt cattgccgcc aatcaaaacg
    10981 atacgtccaa atgttccttt atgtgaaatt tttggacgag gtgtgatgac tcttttcact
    11041 agttgttcgt ttatcagcat ctaaaaacct ccttttactc attatagcgt atattttttt
    11101 tagagaaaaa cagataagaa aaggaattgt cagatttttt gaactatttt tttctgtaat
    11161 ttgtggtatc atgaacacga agacttaaat aagtggagga ttaactttat gacaattgat
    11221 tggcaaaaag aagtagaagc acgtaaagaa gacttgcttg aagacttgaa aaatctttta
    11281 cgtgtcaaca gcgagcgcga tgactcaaaa gtaacaccag atgcaccatt tggacctgga
    11341 cctcgcgacg cattgaaaca tatgttagct tatggcgaac gtgatggatt taaagtaaaa
    11401 aatgtcgata actatgcagg gcatattgat cttggcgaag gagacgaaac gctagggata
    11461 tttggacaca tggatgtcgt tcctgcagga gatggctggg acacagatcc ttatgaacca
    11521 gtaatcaagg atggaaagat ctttgcacgc ggatcaagcg atgacaaagg tccaagtatg
    11581 gcagcttact atgcgatgaa gatcattaaa gaacttgact tgaaactatc aaaaaaagtt
    11641 cgcttcgtag tcggcagcga tgaagaaagc ggctggggcg atatggctta ttatttcgaa
    11701 catgaagaag aaccagattt tggtttctca ccagatgctg aattcccaat catcaatggt
    11761 gaaaaaggaa atgtttcttt agctttacgc ttcaaaggcg acaatgcagg agattatgtc
    11821 ttgaaatctt ttgtttccgg acttcgcgaa aatatggtgc caggaacagc aactgcagca
    11881 cttcaagtac caagtgcaga tgcagctatc gcgatggaag aagcatttta ccaattcatc
    11941 gaagcaaatc cagtaagcgg aacgattgaa gcggacaata cgtatgtaaa aatcgaatta
    12001 gttggtaaag gcgcacatgg tgcaagccca caatctggta tcaatgcagg ttctttcttg
    12061 gcattgttct tagataacta tgaattccta ggatctgcta aacaatttat ccatgtagca
    12121 gctgcttatg tccatgaaga tttttatgga gaaaaactag gagtcgctta tgaagatgaa
    12181 aaaatgggca aattgacaat gaatgctgga ctatttgctt ttgaagaaaa tggtacagag
    12241 gaagcgaact tcatcaatat gaatttccgt tttccaaaag gtgtaacagt agacggattg
    12301 caaagtgaca tcgaacaaac agtggggcaa gaaggcgcaa cagttactcg aggtgctcgc
    12361 gtaatggaac cacattacgt accaatggat gatccattgg tggctacatt attgcaagtc
    12421 tatgaagacc acactggaga aaaaggctac gaacaaatca tcggtggcgg aacttacgga
    12481 cgtttgttga aacgtggcgt tgcttatgga gcgatgttcc caggatatac tgatacaatg
    12541 catcaagcaa acgaatttat gagtcttgat gacttgttca gagcaacagc aatctatgca
    12601 gatgccatct atcgtttggc gaaataatta attaaaaaga ggatcaagaa ttgcaaatta
    12661 tttttgcagt cttgatcctt tttttacttt ctactgtggt attcatgata aacatgatat
    12721 agattcaatt catattcttt cttagcgttc gtgacgactg tatccaaaat caaccctgtt
    12781 actaatgaaa gaagtgctaa agtcatcaat ccagtactta aaatcgcaga agggatgcgt
    12841 gtaataaagc ttgtcagcat atattcgcga atcacaggaa tccccgtgac caatccaaat
    12901 aaaaagaaga agatggtcca aatcccaaag aaaagcaaag gcttataatc tttaaacatt
    12961 ttaataatca tcatgatcac tttgaatccg tcagagaatg tattcaattt tgattcgctg
    13021 ccttcaggac gatcacgata atcgattggg atttcaacga acttgaattt tttatctaaa
    13081 gcatggatcg tcaattccgt ttcgatttga aaaccagaac tcataatagg aaaacttttg
    13141 acaaagatac gattgaatcc tcgatagccg gtcatgacat caagaatttt tgttttatac
    13201 attctagtaa ttgtatttct aacaaggtta tttccaaaat cgtggaaagg tctcttattc
    13261 tcatcaaagt aggttccatt tgataggcga tcaccaatga ccatatctgc ttcaccagaa
    13321 cgtaatttct cgagcaaacc atgtacagct tctgcaggat aagtatcatc gccatctacc
    13381 attaaataat agtcggcatc gatatcaaag aacatttggc ggatcacatt tccttttcct
    13441 tgacgaggtt ctttttttac gattgctcca ccttgaacgg ccaattcgta tgttcgatcg
    13501 gtcgaattat tatcatagac ataaatatct gcttctggta attctcgttt aaagtctgca
    13561 atgactgttg aaattgttgc ttcctcattg taacaaggaa tcagaacagc aattttttct
    13621 tgttgttcca tttttcactc tccttaacta tagaaaacaa aagctaaatt aaaaatacct
    13681 cataatcaaa ggaaatgcta gtttttcccg ctaaatatca tttatttaaa aagaaaatac
    13741 tggaatatat gtttggaaag ctctatctaa aaaaccaccc actacaaaag aaggacatga
    13801 tacctctctt ttgaatagtg ggtgcgtatt cgttctctca cttttttgac gtattaatct
    13861 gttgcacctt tttttcttga aatttctaat acggttcctg tatgagcatc tgctaaaaat
    13921 tcataataaa cagtttcgcc atcttctatt cgagaaagac ctccccgata agcactcgac
    13981 cgaacggcga atttcctaat aggttccttt tcaaaagaga tccaagaacc ttcgattgca
    14041 ccttctttta gaaaagatga cttgatattt tcaagtactt ggtctgctga caatactttt
    14101 ttgcgatgat accaaatcgt tgaagctacc ccgctcaata atcctagacc gattccaact
    14161 gctaacccgc ctttaaaata gcttagttcg ctttcttcat acatgtggac atacctcctt
    14221 cttctcactt gttactcctt gtgcttattt taccacactc tcatattttc tttgaaataa
    14281 ataaccgcta ccagaaaaaa taagctataa taaatacgat cggtctgcag aaaaaatcat
    14341 attttatccg cagatcaata taagtctgag aggagtttag tctgaaattt agacgaatag
    14401 catatggaag aaaaaacatt tcaacgaatc aaagagttaa cagaattaca aggaacaagc
    14461 ggttttgaac aagatgttcg cgcttatatg gaaagtcata tggaaccgct ggtagatgag
    14521 ttacagttag atggtttggg cggcattttt ggtctgcgcc atcatgaaga agcggatgca
    14581 ccccgtgtaa tggtcgcagc acatatggat gaagtcggat tcatgctgac acaaatccaa
    14641 gaaaacgggc tttttaaagt cgtacctcta ggcggctgga atccttacgt tgtttctgct
    14701 cagcgtttta cacttaaaac ctctactggt aagaattatc catgtatttc ttcttccgtt
    14761 cccccacatt tattgcgagg aacaagcgga caaaaatcag tagaagtaac cgatattttg
    14821 ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt acggtgtcct tccaggagac
    14881 acaatcgttc cttacgctga aacgatcaaa acagccaacg gaaagaatat tatcagtaaa
    14941 tcatgggaca accgctatgg ctgtacaatg gtattagaag ctttagaagc gttgcaaaat
    15001 gaaacattag gtcatacatt gattgcaggc gcgaatgtcc aggaagaagt aggcttgcgt
    15061 ggttctaaag cttctgtcaa taaattcaag ccagatctgt tctttgcagt agactgctct
    15121 gcagcagatg atacagtgac gaaaaatggc acattcggac atttaggaga aggtacattg
    15181 atgcgtatcc aagatccagg tttgatcatg cttcctcgtt tgcgcgagta cttgctggat
    15241 atcgctgaaa caaacaatat cccttatcaa tactttgttt caaaaggcgg tacagatgca
    15301 ggtgctgctc atacacaaaa cgaaggaatc ccaagtacag tgattggcgt agtgggacgt
    15361 tatatccata cgcatcaaac gatgttcagt attcgcgatt ttgaagctgc acgagaaatg
    15421 ttgattcaaa cactgaaagg tttggataaa tcaacagtaa atacgatcgt atacggaaaa
    15481 taatgcgata gtgaaactat aaaatctggc aaaagattgc tttttgccag atttttttta
    15541 taaaaagagg atcaatgttt gttagttcga aagaaaggtg tataatttaa ttgaaaaata
    15601 cataaacaga aagtagagga caaagaatga ttatcccaaa tagtttagaa gaattagcag
    15661 gctacgtaga aactggaaag agtgtatttt tctttactgc cggctggtgc ggagactgcc
    15721 gttttatcaa accgcaaatg cctgaaattg aaaatgactt ttctgattgg caatttattg
    15781 aagtagaccg tgacaagtac atagatgtag ccgctgaatg gaacattttt ggtatcccaa
    15841 gttttgtcgt gatccaagac ggaaaagaat taggtcgtct agtgaataaa gatcgtaaaa
    15901 caaaacagga aatcgaatcg ttcctagaaa gtgtaacaaa aggctgacat ctgttaggag
    15961 gggtcaagat gaatcaagaa tataagaata ttttagtagg tatcgacggt agtgaacaag
    16021 cacttgctgc tttcaaaaaa gcggtagaag tggctcgccg taataatggg aaagtatatg
    16081 tagcaaacgt aatcgatcag caattttata actttatggg ctatgcacct attgatcaaa
    16141 atctagtcga tcagcaaact gaaacagcca aggaaatgat cgaagaatgt aaagaatatg
    16201 ggaaatctgt tgattacaca aatatagaag gaattattgc atatggttct gttcgtgaat
    16261 ccatggcagt caaattgcca aaaaaatatg agatcgatct aatcatggtt gggcagtcag
    16321 gtctaaatgc agtcgaacgc tttatgacag gaagtgtagc cagctacgtg atacgtcatt
    16381 caccttgtga tgtcttgatc gtttctgacg aagaagaaca gccaagtaat taagcaagtg
    16441 gaacttgtac gaccttttta atcaattaac taaaacagta aagggactgt gacaaaagtc
    16501 ttgtcacagt cccttgttac gaataaatgg tgttccagaa gaggcttctt cggaaataaa
    16561 gcatgaaaaa aatttcatgc agtgaatgca attttttgtt aagataaatc tcagcaaagg
    16621 ataatggagg aaagaacatg atttttgctt ataacaaagc acacgtcgga gacaccttga
    16681 tggttattat agccgacgat aaaggaacag aaaacactgt tgaacgcaaa tggaatgttg
    16741 ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa ctttttccat atctccgatc
    16801 atctaacaat cgaagggaac ggccaagtaa gcttaacaga tgaggaatta gcgaaattga
    16861 atcgattgat caaagaagca ggattcgaag aacagcttga agctgatcat gaaccgaaga
    16921 tcgttgtcgg gtacgtaaaa acatgcgtcc cacatcctga ttctgaccat ttatcgatta
    16981 ctgagacaga agtagacaat ggtacagtat tgcaaatcgt ttgcggtgca cccaatatcg
    17041 aagctggaca aaaagttgtc gttgcaaaac caggagctat gatgccagat ggaatgatga
    17101 tctggccagg aactttgcga ggggtagata gctatggaat gatctgttcc gctcgtgaat
    17161 tgcatttgcc aaatgctcca gcaaaaaaag gaatcttagt attgcctgag gatgccgtag
    17221 tcggagaagc atttccaaaa taaagaggtt gtgacaagtc tttgtcacaa cctctttttt
    17281 attaattttc actattttga ttttgtttta atgccgattg atcgatcgtc aattcaacag
    17341 tagttgtttt ttctttcgag ccttcatagt aagtgatttt gacagaatct cccacttttt
    17401 tcttatacaa agcagattga agttctacac cagaagaaac ttctttgtca tcgatcttag
    17461 taatgacgtc gtattgtttc aatccagctt tttcagccgg tgtagctgtt tggacatttg
    17521 tcacgatgac accgttagtt acagatgaag gaatcttcaa gatttgttct tgttgctgag
    17581 tagaaacggc agataagtcg accattgtga tacctagagc tggacgtgtc acttttccgt
    17641 ctttttccag ttgattgatg atattcacta catcgttact tggaatagca aatcccattc
    17701 cttctacgct tacattagaa gtagattcag aagtacttgc gatcttactt gagttgatac
    17761 cgataacttg tccttcgata ttaactaaag gaccaccaga atttccaggg ttgattgctg
    17821 catctgtttg gatcgcattg atattgactg tttcgttcga ttcattcgta cttgtcactt
    17881 gtcggttcaa ggaagaaatg attcctgaag tgacagaatt tgcatattca gatcctagtg
    17941 gggaaccaat cgcaatcgca ggctcaccaa ccttcagagc acttgagtca ccaaaagagg
    18001 caactgtttc gactttgtct gaattgatct tcaacaccgc taaatctgaa taagcatctg
    18061 ttccaactaa ttcagctttt accttcgtac cgtctttcat caagacttca agaccttgct
    18121 ggccatctac tacgtgatta ttcgtgacta cataagcagt attcccgtct tttttgtaga
    18181 tcacgccgct tccttcacta taagcttcca aattactatc atctgagctg ctactttctt
    18241 cttgctgccc gaatagtccg ccaaaaccgc ttgattggtt ttgactttgt aagttgataa
    18301 cagaaacgac agcgtcttga actttatcta ccgctttggt gatatcagaa tctacattga
    18361 ctttgacatt ttccacaact gtttcaccgg cagaattctg attgccgctt gtagcagtag
    18421 aactattgtt gccagtgccc atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa
    18481 cgatccctcc aacaagcccc agacctaact tacgccagag gctattattt ttactttttt
    18541 tcatctttgg tgtcacattt tttctatcca tgtgttcctc ctccaattac attcatttat
    18601 ctactgttag tatagtctta tttgttgact ttagtctact ttgagactgt gaacttttta
    18661 tgaaaaactt taggaaaaat cattgttttc agatgaaaat atttgatttt ct
//
LOCUS       DAZKNM010000044        18455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000044 DAZKNM010000000
VERSION     DAZKNM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.044"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..515
                     /locus_tag="ACG8M9_001198"
     CDS             81..515
                     /locus_tag="ACG8M9_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0377984.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(593..1126)
                     /locus_tag="ACG8M9_001199"
     CDS             complement(593..1126)
                     /locus_tag="ACG8M9_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0377985.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="ACG8M9_001200"
     CDS             complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="ACG8M9_001200"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0377986.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2391..3188)
                     /gene="recX"
                     /locus_tag="ACG8M9_001201"
     CDS             complement(2391..3188)
                     /gene="recX"
                     /locus_tag="ACG8M9_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0377987.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3295..4677
                     /gene="rlmD"
                     /locus_tag="ACG8M9_001202"
     CDS             3295..4677
                     /gene="rlmD"
                     /locus_tag="ACG8M9_001202"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0377988.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4851..5429)
                     /locus_tag="ACG8M9_001203"
     CDS             complement(4851..5429)
                     /locus_tag="ACG8M9_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0377989.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5751..6221
                     /locus_tag="ACG8M9_001204"
     CDS             5751..6221
                     /locus_tag="ACG8M9_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0377990.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6329..7555)
                     /locus_tag="ACG8M9_001205"
     CDS             complement(6329..7555)
                     /locus_tag="ACG8M9_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0377991.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8309..8509)
                     /locus_tag="ACG8M9_001206"
     CDS             complement(8309..8509)
                     /locus_tag="ACG8M9_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0377992.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8643..9251)
                     /locus_tag="ACG8M9_001207"
     CDS             complement(8643..9251)
                     /locus_tag="ACG8M9_001207"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0377993.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9336..9806)
                     /locus_tag="ACG8M9_001208"
     CDS             complement(9336..9806)
                     /locus_tag="ACG8M9_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0377994.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9854..10768)
                     /locus_tag="ACG8M9_001209"
     CDS             complement(9854..10768)
                     /locus_tag="ACG8M9_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0377995.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10914..11717
                     /locus_tag="ACG8M9_001210"
     CDS             10914..11717
                     /locus_tag="ACG8M9_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0377996.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11895..12842
                     /locus_tag="ACG8M9_001211"
     CDS             11895..12842
                     /locus_tag="ACG8M9_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0377997.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12910..13203)
                     /locus_tag="ACG8M9_001212"
     CDS             complement(12910..13203)
                     /locus_tag="ACG8M9_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0377998.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13231..13896)
                     /locus_tag="ACG8M9_001213"
     CDS             complement(13231..13896)
                     /locus_tag="ACG8M9_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0377999.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14036..14668)
                     /locus_tag="ACG8M9_001214"
     CDS             complement(14036..14668)
                     /locus_tag="ACG8M9_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0378000.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14675..15742)
                     /gene="liaS"
                     /locus_tag="ACG8M9_001215"
     CDS             complement(14675..15742)
                     /gene="liaS"
                     /locus_tag="ACG8M9_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0378001.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15739..16470)
                     /gene="liaF"
                     /locus_tag="ACG8M9_001216"
     CDS             complement(15739..16470)
                     /gene="liaF"
                     /locus_tag="ACG8M9_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0378002.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16639..17118)
                     /gene="greA"
                     /locus_tag="ACG8M9_001217"
     CDS             complement(16639..17118)
                     /gene="greA"
                     /locus_tag="ACG8M9_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0378003.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17254..18429)
                     /gene="mltG"
                     /locus_tag="ACG8M9_001218"
     CDS             complement(17254..18429)
                     /gene="mltG"
                     /locus_tag="ACG8M9_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0378004.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5488 a         3735 c         2970 g         6262 t
ORIGIN      
        1 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
       61 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
      121 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
      181 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
      241 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
      301 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
      361 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
      421 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
      481 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
      541 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
      601 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
      661 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
      721 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
      781 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
      841 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
      901 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
      961 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     1021 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     1081 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     1141 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     1201 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     1261 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     1321 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     1381 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     1441 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     1501 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     1561 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     1621 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     1681 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     1741 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     1801 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     1861 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     1921 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     1981 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     2041 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     2101 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     2161 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     2221 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     2281 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     2341 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     2401 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     2461 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     2521 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     2581 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     2641 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     2701 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     2761 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     2821 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     2881 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     2941 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     3001 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     3061 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     3121 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     3181 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     3241 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     3301 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     3361 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     3421 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     3481 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     3541 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
     3601 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     3661 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     3721 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     3781 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     3841 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     3901 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     3961 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     4021 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     4081 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     4141 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     4201 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     4261 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     4321 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     4381 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     4441 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     4501 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     4561 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     4621 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     4681 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     4741 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     4801 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     4861 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     4921 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     4981 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     5041 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     5101 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     5161 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
     5221 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
     5281 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
     5341 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
     5401 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
     5461 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
     5521 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
     5581 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
     5641 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
     5701 ttgctgtgag ccttcatggt aaactaatat tatagaaagg agttagtaaa atgaagatga
     5761 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
     5821 ggttgcttag cggaaatccc ggaaaatatt actattcttt gcaacttcca ccatttgcac
     5881 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
     5941 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
     6001 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
     6061 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
     6121 tgtggctgca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
     6181 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
     6241 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
     6301 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
     6361 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
     6421 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
     6481 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
     6541 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
     6601 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
     6661 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
     6721 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt
     6781 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
     6841 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
     6901 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
     6961 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
     7021 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
     7081 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
     7141 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcgtaggca tgatttaatt
     7201 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
     7261 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
     7321 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
     7381 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
     7441 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
     7501 tcagcaaaac gaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
     7561 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
     7621 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattatta ttttttaaaa
     7681 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
     7741 gcagttcgca atcgttttat ctttttttat gtattttttc cagaaaaagc gaacaacaag
     7801 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
     7861 gtctttgcct accgttcaga ttatcattcg atttcacgac aatttcttcc tatgactggt
     7921 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
     7981 tgactcgaac tagtgattgc attaccatca tcttttaatg gatagatatg ctcctcttca
     8041 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
     8101 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
     8161 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
     8221 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
     8281 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
     8341 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
     8401 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
     8461 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatatc
     8521 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
     8581 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
     8641 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
     8701 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
     8761 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
     8821 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
     8881 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
     8941 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
     9001 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
     9061 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
     9121 tcaattgctg aatttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
     9181 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
     9241 gtgtaagtca taaaaaaatt cctcctcaat gttttcttaa taatgtctac attacaaaga
     9301 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
     9361 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
     9421 gcaaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
     9481 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
     9541 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
     9601 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
     9661 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
     9721 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
     9781 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
     9841 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
     9901 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
     9961 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    10021 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacatcct
    10081 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcgata gcaggtgcac
    10141 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    10201 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    10261 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    10321 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    10381 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    10441 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    10501 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    10561 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    10621 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    10681 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    10741 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    10801 taacattttt ttgatatttc accaatcatt cagctgtttc cttaaagcac ttgtatttca
    10861 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    10921 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag gcgagaaatg
    10981 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    11041 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    11101 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    11161 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    11221 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    11281 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    11341 aacttcccta caccaatgaa tctttaaaaa acttaacagg taagcagctc cgttcttttt
    11401 taagtgaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    11461 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    11521 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    11581 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacta
    11641 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    11701 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    11761 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    11821 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    11881 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    11941 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    12001 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    12061 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    12121 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    12181 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    12241 aaaaggcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    12301 ggaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    12361 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    12421 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    12481 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    12541 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    12601 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    12661 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    12721 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    12781 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    12841 aatagctgaa aacaatattt tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc
    12901 tctctttttt tattgttttt taaagtgata ggttggctcc tccaggttgg gatcataatc
    12961 aacaagtaaa tcatatcctt taaagaacca ttcatctgct tcctcgataa aaaaaagtgt
    13021 cccatcaatt tccactttct ttacaggatg ttctggctgt tcaactgaca ttccaatcga
    13081 aaaaccatca tgaacctctg ttttgccgta gacttttcca aaaaaacgta ttccgtaagt
    13141 ttctggcaga tcaatttctg agtcaaacca cttttgtgct tttggcgtta ccgttaattt
    13201 catcccaaca acctactttc tcttttcttt ctattgattc atcttatcgt ctagaatatc
    13261 cacatcgcct gtctctccaa taaccagtag attatcattt tcacgaacat attcatctgc
    13321 tgcaggagaa gcgataaccg tttgatctac tcttcgtatg gcgactactg ttaagtcaaa
    13381 acgttgcctg aaatttaatt cagctaatgt tttgttgaaa aatttaggat tggagactcg
    13441 gatttctgct aaagaatatt tatctgacaa ctctaggtaa tctaaaatat tctttgacac
    13501 taaattatgc gcaatcctta ttcccatgtc tcgctcggga tggacgacgc gatcagctcc
    13561 gatcttttca agtactctgg catgatactc attttgggct tttgctaaaa tatttggtac
    13621 gcccatttcc tttaccatca gagtaactaa gatactggct tgaatatctt ctccaatagc
    13681 gacaattaca tgatcgaaat ttcttatgcc taatgatcga agtgtcattt catcctgtgc
    13741 atttgccaca acagcatgag tggctatatt catatattca ttgacccgat cttcattact
    13801 atcaatagcc agtacttctt gaccagcttc gatcagtgtt cggcagatac ttccgccaaa
    13861 tcgtccaaga cctacaattg caaaattttg tttcaccgtt ttcctccttt agtatcttct
    13921 tttctcagta taacaaagcc tttacaaaaa caaaagcaag catcattatt gtaaaaaagc
    13981 caagaaaaat aatagttatt ttctcagctt ttgattatcc tctttatcat cgtttttatt
    14041 tcaccaaacc atgtttgaat gcatagatag cggcttgtgt acggtcttcc acttctagct
    14101 tagccaatat attggaaaca tgtgttttta ctgttttcag tgtaataaac aattcgtccg
    14161 ctatttcctg attacttttt ccttcagaaa tcagcattaa aatttcattt tcccgattcg
    14221 ttagttcttc atgtaataca ggctcatttc gccgactcat tttttccatc atttttgttg
    14281 tcacttctgg ttctaatact cgctcgccac gttgcgttgc ccttatagca tcagcaattt
    14341 catgtgcggt agaggtcttc aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa
    14401 ctttttcgtc atcaataaaa ctggttacga taatgatttt agcttctggc caatctttta
    14461 agattgcttt tgttgattcg ataccatcca tttcttccat gactaagtcc attaaaatga
    14521 cgtccggtcg aagtgtcatc gccttctcat agccttgtcg tccattttca gcttctccaa
    14581 ttacttcaat gtcttcttga atagataaat aaaaagagac gcctaagcgc accatttcat
    14641 ggtcatctac taataaaact tttatcatcc ttatctatgc ctccttcatc aaagggacct
    14701 tgatttctac acttgtacct tgacctttaa aactaatgat ttttactgtt ccaccaatac
    14761 cagctactcg ttctttgata ttgttcaatc cataacttcc cgttttcgtt tcattcatat
    14821 taaaccctgt tccgtcgtca ataatacgta acaaaagatt attgtctatt ttgtgcaaat
    14881 atacctctaa ttcattggct ttagcatgtc ttaatgtatt tgataacaat tcttgaacga
    14941 ttcggaacaa gtgatcctca atggaactag atagttttac atcttctaca tcccatttca
    15001 gcgaaatctg gattttattt tgcaattcct tcaataactg ctcgatacct tgctttaaac
    15061 ttttctcttc taaattgact ggacgtagat gaagaagcag cgcacgcatc tcagactggg
    15121 aagcgttgat gatctctgct accattttca actgcttgcg aaacatttct ggtgtctcgc
    15181 ttttttctgc ttgctccgtc aatgccgaca tcatcatcat tgctgcaaac aattgttgcg
    15241 aaactgaatc atgcagctca cgggctaatc gatgtctttc taattctaaa atttcttctt
    15301 tggtttgtcc atctacatat tggggacggc tcgttacaat ttgtaattca ctagatactt
    15361 caatcatttt ttcttttatc ttagtgattt ccttatcaat atctttgatg tataaatcgt
    15421 ccgatgcact tgggatcgga agaagcaaca atttggattc ataattgcca gaagaaagtg
    15481 cacgcagctt ttcttcgatt ttcccgtatt ttgtcttttg aataagtgat aataatagaa
    15541 agacaattaa tcctacacct aaggaaatgg ctaacaaatg gaagataagc ggaacatata
    15601 ataaacgtgc acgcagtaat tctccccacc aatgactttg attgctggca taaaaataag
    15661 tgaaaagtga aaatagaata ataaggaaag ccgcaatccc cgaataaaca gctagcatcg
    15721 ctctggatat ttttcccatc atattcgtat cacctcgaca tctccaatca aggtattagt
    15781 aataatcttc aatctacgag ggttttcatc ataatcactg ctgtagattt tgatttgttc
    15841 atttttcaaa gaaaattgct cttcttcaaa caagacattt cctactaatg tcgcatgctc
    15901 taatcggatg gctacgccca aaggtactaa gatccgtgtt ctgcctatcc cttttcttac
    15961 aatcacaata ttatcatctt ttggcaaaag cgtattgccc aaatctatga tagtatcacc
    16021 agaaatgacg gcgatgttga tatcgtccca ttcatagacg tcgtttccaa tacgttggtt
    16081 accgaataat tgctgtcttt tccgctcatt attatgtgtt tttaattgct ctgtttggat
    16141 catcatgatc tgtttctttc gccaaaaagc atttttggtt aaatctatcc ctgaaatctc
    16201 tacacctttc agaccaataa ataaaacggc aaatacagcc atcatccata aagcaggact
    16261 gttgactaag ctaaaaaaaa tgaccaaact tcctaatatc aattgaaaat tttgaaattt
    16321 agtacgagga tgtttgcgca tagctagata tatattgaaa ataccgaata tcactaaaag
    16381 caaaagtcca gtattgttta cgatttgcca aacagcaaat aacagcagta gtgcttcaac
    16441 taccacaaaa aaacgccaag aactattcat cgctctttct ccctcctgac acattctcat
    16501 ttcatcatac ctattttttt aactgttggc tataggactt tagatgtaag atcgtttgtt
    16561 gaaatctaaa atacacaaag agaaaaagat ctagagtgta tcatcagtaa gatgaactct
    16621 ctagatcttt cagtcagact acgcttgttc tactttgacg attttaacta acatatcgcc
    16681 acctggagta gcaatagtta cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat
    16741 aggagaatca ttggaaattt tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt
    16801 gtactcttct tcttctccat ctggtagttc gatgaatgta actgttttac caattgatac
    16861 ctcatctgca tctacaccgt cgttatcgat gatttgagca aaacgaatca tattttcaat
    16921 tgttgtgatt cttccttcaa caaatgcttg ttcatctttc gctgattcat attcagagtt
    16981 ttcggaaaga tctccgaaac cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt
    17041 gactgttttt agttcttcta attctttttc taatttttct ttaccttcta gagtcattgg
    17101 ataaactttc tctaccatgt gtaacgtctc cttttttcga taaaataccg caaaaggtaa
    17161 tcttttgagg aatatagtcc aaaaaagatt accacgcttt tcttcaaatg taaatgtttt
    17221 tctaaaaatg atcaatgtct agagcctgtt tttctaagaa ttatttacat atttttccac
    17281 taattccata tgttcttcat aagttttagc gaaataaaca tttccagtgc tcgtatctgc
    17341 tacgaaataa taatagtcat tttctgctgg ttctaatact gccttgattg cctcttcgct
    17401 tggattgtca aaaggtccag gtccatagcc ggtattggta tacaaattat atggtgaatc
    17461 tacagctgta tcttcatacg tcactaattc tttgtgttca cctaatgcgt ataaaacaga
    17521 gatatctgac tgaagtggca tatttgcttt gattcggtta aagaagactt gtgcaatgtt
    17581 tttacgatca ttttctttta cgccttcttt ttcaactaga gaagctagag ttaaaacttc
    17641 ttgtacagtc aaatttttct gcttgatagc tgaataatag ttttgcatga ccgtattcgt
    17701 cttgttcacc atttcaatca ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt
    17761 ggctgggaat aagtaacctt ccaaacggta ttttgtgttc tcagctttag atgcactttc
    17821 caacagttct gggaattttt ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc
    17881 taaaaattct ttcttgtctt taccagttac cttcgctacc cgttcagcaa tctgatcgat
    17941 atcgtaccct tctggaatgg caattttccc atctgcaact tttgttggct cagaagtacc
    18001 gccttcttgc aactgtttgc caatctcgtc aagagtcata ttaggcgcta actgataata
    18061 gccagcttga aagccagtta aatttttaaa ttttgtataa taattgaaaa caatcccgct
    18121 tttaataata ttgtccttct ccaaaatctc accaatctgc ttgttagaag agccactcgg
    18181 gatctcgact tggactaatt ggtcatctga tttatctaaa ggcttcaacc cgctatctac
    18241 ataacgatac acggtaaatc caagaaatcc gccaataatc aataatgtaa gagcaatcac
    18301 taaaatgatc ttcctcacaa tcttatcttc ttttttacgc agatttctcc gcttgtcgat
    18361 tatatctgct gtagctgcct tactggcttc ttttttatct ttacttgggt gttgtttgtt
    18421 tcctgacaaa acaactcctc cttactatca agttc
//
LOCUS       DAZKNM010000045        18402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000045 DAZKNM010000000
VERSION     DAZKNM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.045"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..311)
                     /locus_tag="ACG8M9_001219"
     CDS             complement(87..311)
                     /locus_tag="ACG8M9_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378005.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(331..753)
                     /locus_tag="ACG8M9_001220"
     CDS             complement(331..753)
                     /locus_tag="ACG8M9_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0378006.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(895..1605)
                     /locus_tag="ACG8M9_001221"
     CDS             complement(895..1605)
                     /locus_tag="ACG8M9_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0378007.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1623..2120)
                     /locus_tag="ACG8M9_001222"
     CDS             complement(1623..2120)
                     /locus_tag="ACG8M9_001222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378008.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2157..2609)
                     /locus_tag="ACG8M9_001223"
     CDS             complement(2157..2609)
                     /locus_tag="ACG8M9_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0378009.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2744..3349)
                     /locus_tag="ACG8M9_001224"
     CDS             complement(2744..3349)
                     /locus_tag="ACG8M9_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0378010.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3799..4419)
                     /locus_tag="ACG8M9_001225"
     CDS             complement(3799..4419)
                     /locus_tag="ACG8M9_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0378011.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4421..5290)
                     /locus_tag="ACG8M9_001226"
     CDS             complement(4421..5290)
                     /locus_tag="ACG8M9_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0378012.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5313..5720)
                     /locus_tag="ACG8M9_001227"
     CDS             complement(5313..5720)
                     /locus_tag="ACG8M9_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0378013.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5739..7028)
                     /locus_tag="ACG8M9_001228"
     CDS             complement(5739..7028)
                     /locus_tag="ACG8M9_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0378014.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            7086..8425
                     /locus_tag="ACG8M9_001229"
     CDS             join(7086..7527,7527..8425)
                     /locus_tag="ACG8M9_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0378015.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            8494..9096
                     /locus_tag="ACG8M9_001230"
     CDS             8494..9096
                     /locus_tag="ACG8M9_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0378016.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            9118..9606
                     /locus_tag="ACG8M9_001231"
     CDS             9118..9606
                     /locus_tag="ACG8M9_001231"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378017.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            9928..11301
                     /locus_tag="ACG8M9_001232"
     CDS             9928..11301
                     /locus_tag="ACG8M9_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378018.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            11285..11722
                     /locus_tag="ACG8M9_001233"
     CDS             11285..11722
                     /locus_tag="ACG8M9_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0378019.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(12002..13747)
                     /locus_tag="ACG8M9_001234"
     CDS             complement(12002..13747)
                     /locus_tag="ACG8M9_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HFQ0378020.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMTNRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            13943..15019
                     /locus_tag="ACG8M9_001235"
     CDS             13943..15019
                     /locus_tag="ACG8M9_001235"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0378021.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            15172..16524
                     /gene="murF"
                     /locus_tag="ACG8M9_001236"
     CDS             15172..16524
                     /gene="murF"
                     /locus_tag="ACG8M9_001236"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0378022.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            16846..18357
                     /locus_tag="ACG8M9_001237"
     CDS             16846..18357
                     /locus_tag="ACG8M9_001237"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0378023.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
BASE COUNT         5613 a         3471 c         3543 g         5775 t
ORIGIN      
        1 ttatctcaaa gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata
       61 aagtttcttc attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc
      121 tgttcttctt tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa
      181 cctgtatcga ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt
      241 gctcgaagct tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc
      301 attagatcca ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac
      361 aaaataattg ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat
      421 ttctcctggt tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa
      481 agaaggcggt ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac
      541 gcctaattct aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc
      601 gattgtttca gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac
      661 gtcattgaca taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt
      721 tgattttctt tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt
      781 gtttgatact attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac
      841 tttttttaca aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa
      901 ttctgcttct gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg
      961 atcgattttt tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt
     1021 gatggattca atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc
     1081 ttcacgccag ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc
     1141 cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg aatgattcgc caccttgggc
     1201 ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc
     1261 aaaagacaag gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg
     1321 ttcttgattt gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt
     1381 gaatgtggta aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc
     1441 tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc
     1501 actttctttt gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc
     1561 aggaaatact tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt
     1621 gattagaata attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa
     1681 acatgttcgc tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg
     1741 tgttcccaaa caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct
     1801 gtatctagta aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc
     1861 tcgatttcta aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc
     1921 aggtaacccg caggctcttg atcgatactc actaggaaaa aatcggtttc tttatctttt
     1981 agctcttctg taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc
     2041 ggtgaattat cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag
     2101 tctacatctg aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa
     2161 tagatccttt tattgccttt tttgacaaaa ttccaatctt cgctaacatt cttgcatact
     2221 ttatttaaaa gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact
     2281 gcattggagt agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc
     2341 ggatagagtt tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg
     2401 gattctaatt tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct
     2461 agtttttctt ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct
     2521 cttgttaaat cgacttcttt gaattcgata ttgctaatag aatctagcgc tcgggcaatc
     2581 attccgatgt ctcgaaggat ttcagccatt tcatcccacc tttattttgt ttgtaagact
     2641 agttcacctc tttttgttgc aaatgcaact attttcaata aaaaaatcaa gttctttttt
     2701 aataactagt aaagtatatt cttttcttct tttacgaaaa agctcaatca caatagatct
     2761 ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt tcgcttttct gatagctttg
     2821 gcaactcttc tatactaaaa aatcccatgt tggaagtttc attattctca ataaacttgg
     2881 cctcttcacc gtggatcgca caagcaaaaa caagtttata atactgaaaa agctgtggaa
     2941 tatctttttg tttattcgta tcaaaaactg ctcgcaactg agtagtttct actgttaatc
     3001 ctgtttcttc atagacttct ttacgcacat tttcttcagg agacaatcct atttcagcaa
     3061 atccaccagg aagtgaccat tctttcgtcc gtttatcttc gattagtagg accttgccat
     3121 cttttttgat ataggcacga acatctactt ttggtgttgg atgtccttct ccaatcgttg
     3181 tgagcgcttt tatttcttcg aaagtttctt ttcctatttc actgatcagt tccaatgaaa
     3241 tcgtccggat ctcctcaaaa cgctctttat caaatacatc ttttccataa tatagaccag
     3301 cttgagaaag tgcgagtagt cttttgtatt gagccaactt cttgttcatt ctcttccctt
     3361 cttttttagc aaatgtatga ctatgtaatc aagtatagaa gactggttta attacggcaa
     3421 ataaatataa tgaaatctgc aataaataaa agaatgttaa aaaactcgag aacaaaagac
     3481 attcgacttc tctggggctg tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa
     3541 gccccaccaa acgaaaaata tgacgagcta ttcttgtctg gctgttctta ttaattgagg
     3601 caggacagct ttttcacaac ctccccacta aacggtgttc tcctaccaac tcctcggtca
     3661 caagtcacaa tattcatcaa acatgagaag ctgttttttt gaaattgctc cttgtgactc
     3721 ggagctgaac gacaggctca cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga
     3781 tattaagcca tttctttgtt acttattgtc attgatttct ttttctaatt tatccaatag
     3841 tggagtgtaa tccacttgag tatagtctgt tacatcaaat tcatatagtg ttcctaactg
     3901 gaatttgtca tattttcggt cggcgatgat ctttttgaat gcttctttcg taatatgatt
     3961 ggtccctttc gttcgatctt ccaatacgtc accatagtga taatgatcca tgatatagct
     4021 caaatgacga tctgattcac gatcacgttt gtaccgacgt tgccataaca cttcaaagtc
     4081 tgctgttagg cgaatcgtga tcacctgata tccgtatcta tctgctaatt cttggagtcg
     4141 tgctttttgc ttatcactaa aaggatactc ggacacaatc actttctttc ctgcttccat
     4201 atacaaatca agtgctttgt aatagaaagg ataaactttc gtcctttctt gttccgcccg
     4261 ttcttccaaa ttgttaaacc cgatggattc tgcatagtat tccttgattt catcgagcgt
     4321 gatggcatac atatctggaa atcgttcccg tagtttattc ataagatatg tttttcctgt
     4381 accaggacta ccagccagaa gaatgaaata ctttttcatc tcatttattt cctaagttaa
     4441 ttggactttc caagttcata aacagtccaa tcttatgctt gacaatttct tttactgctt
     4501 catttgctgg aatcacatta tggcgaagag acttgttgat ttctgtttca ctaaaggttt
     4561 ctttagctac tttcgtccag ttgactaaaa gctccgttcc aacattgaat ttgcggatcc
     4621 cgttattgat caattcagga taatcttctt ctttcacacc tgttcctcca tggataacta
     4681 gggggacatc aactactgca tcgatttctt tcagcaaggg aatgtttact tctgtttttg
     4741 atttgaattg tccatgattc gtaccgatag caatagctaa tgcatccacg cctgtagctt
     4801 ctacaaattt cactgcgtct tccggtcttg tatatacctt gtcattttca gcgacatgga
     4861 ttccttcttc tgtaccgccg attgttccaa gttctccttc tacggaaaca ccgtgcgcat
     4921 gggcatattc tactactgct tttgtcttca ggatattttc tttgaaaggc agatgggacc
     4981 catcatacat gacagaagta tatccgctgt cgattgcttc tttgatatca tcaaagtctc
     5041 gggcgtggtc taagtgcaag actgcatcaa ccatttcttc ttctgcaata gctgtcatta
     5101 cattgactaa cacatgcatg ccgatatatt tagcggtatc gacacttgtt tgaataatga
     5161 tcggcgaacc catttctttt gctgcgccta gcatagcagg aagcatttct aggttatgcg
     5221 tattaaaagc tccaactgta aaattcaact cttctgctgt ttttgttact tctttcaatg
     5281 ttgtatacat cttttattgc caccttatct ttttatttgt ttcctttgat cgttacttgt
     5341 tttgcaattt gtcgcatctc atccattttg cccatgtagt aatcgatccc ttgatcatca
     5401 ccagcacgag cagcatctgc acgcttctgg ttatagaggc tcatcagttt tttataccca
     5461 tctccgatcg tctgtttatt ttccaaactc ttttctaaat attggatggc ttgatcgttc
     5521 acatacaatt cagaaaattt aagtccaact tgttcataaa gtttcgctaa tttatgctta
     5581 tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc
     5641 gcttctggcg atagggcttt ttcttctttt gccagaactt ttttcggttc tttttctttt
     5701 ttcttaaaaa atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg
     5761 cttaagatcc aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca
     5821 ttgttgaatc ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag
     5881 aaaccatgag caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat
     5941 atccctgctg tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag
     6001 ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct
     6061 aaaatgacag catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca
     6121 tcaggaacta atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga
     6181 cgtacaccag ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca
     6241 aacatgatat agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa
     6301 acagtgatga tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga
     6361 aattcgaaat gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca
     6421 ccaaaaagct tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa
     6481 gcaatatcat ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca
     6541 atggcgaaac aagctccgat aaaaccaccg accaagatca atacgatccc tcgcagaccg
     6601 gcaaaatagc caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa
     6661 aaaatgtact tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag
     6721 aacgtcagtg caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat
     6781 tcaatagaag ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa
     6841 gttaaacttc caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc
     6901 tttaacgtgc cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata
     6961 gcaatcaatg caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata
     7021 acgcccatgt tttgatgtgc accccaaaag ttagactaga aatctaatta aaaggggtgc
     7081 tttttttgcg gaaatataca tttgttttta agaaaaaagt agtttcagac tatttaaata
     7141 acgaaggcgg ctacaaatat cttgcacata aatatcaaat aaatcgtaca ctggttagac
     7201 attgggtaag gatttataac tatcatggtt gggaaggctt ggttggaggt ggcaaaagct
     7261 acactacaaa atttaaactt gatgttatag aatatatgga aacaaatggt ctttctatcc
     7321 aagaaactgc taaaaaattt aatatcggtt caaatagaac tctaagtaaa tggatagagc
     7381 aatatgaaga aggcggtgct tcttcacttg agagccaaaa aaggggcaga aaaattagta
     7441 tgaattccaa gctaaacatt cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta
     7501 ttcgtttaag agcagagaac gcatatttaa aaaagttaga aaccttgatt caagaacaag
     7561 atttgagcaa gaagaaatca aggttaaaac aatccttcag ctcaaaaaag aatttaaatt
     7621 gaatctatta ctttcaattg cacaattagc gaagtcaact tattattact gggtaaaaaa
     7681 attagataag ccagataaat atagcaagat aaagcaagaa attacagcga ttgtaaaaga
     7741 atcaagaaac tcttatggtt atcgtagagt cactttagcg ttaaagatga aaggatatac
     7801 aatcaatcat aaaacagtta gaaaattaat gtcccaaatg ggacttacct gtcaaatccg
     7861 aataaagcga tataaatctt ataaaggaac agtaggaaaa attgccaaga atgtgttaaa
     7921 acgaaatttt tcagtagata cacccaataa aaaatgggtg acagacgtca ctgaattcaa
     7981 gataaaagga agaaaaatct atctatctcc tatacttgat ttatttaacg gagaaataat
     8041 tagttatagt atatcaacta gtccaacata taaactgatt gaagaaatgc ttcaacaagc
     8101 aattaaaaaa aagggaactg agggttcact gattctacat tcagatcaag gatggcagta
     8161 tcagatgcca caatatcaaa aaaaattaaa agagaataac attattcaaa gcatgtctag
     8221 aaaaggaaat tgcttagata attctgtaat agaaaatttt tttggtgtgc ttaaatcaga
     8281 gtttttttat cgagaaaaat tccgatcaat tgagatattt caaagtaaat taaatgagta
     8341 tattaggtgg tataataaca aaaggataaa actaaagtta aatggactat ctccagtaga
     8401 atatcggaaa cagtccatta aatagttcaa cttttggggt tcagttcata aggaggcagt
     8461 tttaataaat gaaaaaagat gtggggaata tagatgtcta aaaaaggatc agttttacaa
     8521 aaatgtcttc agatcatgcc cattattggt ctgttttttt tctttgtgtt gattctttat
     8581 gcttatcatc atgggatttt tcgatcgagc acgtctttgc aagcgtttat ccagcaattt
     8641 ggcaagtatg cagtgatgat atttgttctg ttgcaagtga tccaagtgat cattcctatt
     8701 ttgccgggag ggatctcctc agtagcgggc atgctgatgt ttggcaatgg atttgggctt
     8761 ttatatagtt gtattggcct agtgattggg gaagcaatcg gatttttgct tgtccgttac
     8821 tatggcgtag cctttgtcca gcttatttta tcgccaaaaa agtatcagaa atttgatgaa
     8881 ttgctgacaa gaaaaacgaa agatattaaa aaagtactag ttgttacctt gctgcttccc
     8941 tttgcgccag acgacttgat ctgtctagta gctggtttga cgaaattatc ttttagggaa
     9001 tttatgcaga ttgtgatttt cttgaaaccg tggtcggtgg gtgtgtatag catgatcatg
     9061 ctctttttat tccatcaagc gcaaggtcat ttataatgta aaaaaggacg tgggtgtatg
     9121 gaaaaaataa aagtaacaga aaatgaactg gatgagctga tggcagtcat acaagaagta
     9181 tggccggaag tttttgtgcc gatcattggt caaaaacaag tggattacat gctgaaaact
     9241 tatcagtcaa aaaaacagat tcaaaaagaa ctgacagaag gtgtgtccta ctttttatta
     9301 aaaagcgaag aaaagactgt cggttacact gcttatgaag agataaatgg aaaaatttat
     9361 ttaagtaaat tatatttgct aaataaagta cgtggtcaag gattgacttc ttcagttttt
     9421 cgctggtatg aagaacttgc tgcgcaaaca aaggaaagag agatcttttt gcgagtgaat
     9481 caggggaatc atcaagcaat cgaggtttat aagcatgaag gatttgagat tacagaagaa
     9541 cttatttcag atatcggtca aggatttcaa atggtcgatt ataaaatgaa aaaagctctc
     9601 gcataaacat aggaactgtg ttaaagtaaa tttatgcgac gggtcgagaa ggactctggt
     9661 ttacagagct cctaattgat tttggaggca caggataatt gatttctatc tttatcacct
     9721 gccggatgca ggggtgtcgg gcaagacttt tgtggagaag ggcttgtctg cttgagtaat
     9781 caagtaccgt aaaaatgcag aaagaagctt atgcttcttt ctgtattttt ttttacaaga
     9841 atatattctg taaaattggt ttatttcccc tttaatgcta aaatcgaaga gcagattagt
     9901 tataagactg tttaaggagc cattgtcatg ttcgaaaaac tcaaacgatt tgggcgtaat
     9961 tttacacaaa caacgccctt tatcttaagt aacacggtta tttttattcc ttatctttta
    10021 tttttgggac taacagactc gcataattgg ctgacgattt tgccatttac gctgttttat
    10081 gcctttcgga tgactggact atttttagtc aacagtctac gatttggtct agatagttac
    10141 acgttgctga tgatcgctct gctgattggt ggtgctggtt cattgcttgg aatattaggc
    10201 gtattttatt ttcctttatt ttatttttca agtgttttgc tagggctgag tgcagcatgg
    10261 cttgtacctg ctaataccac ggtcaatttt cacgaaaaga cgcaaggttt tatcaatatg
    10321 gatgggaaaa agtacttatt tgcagctatc tggctatttc tcctgtatca agcgattgag
    10381 ttgcctgcac cgactcagat tgcagcaagc tttactattt atacactttt ttacgtcatg
    10441 gcctaccata cggttagtca ttatccgcgt tacgaactgg atttcaaaga catcaaaaga
    10501 aatctggtag ctacgcgaga gcttgtttta tttttgatat tctttggctt attgtttctt
    10561 ttgagaaatg cccgattgtt attcgatgaa aggctttttg atcttgctat ttatggtttc
    10621 ttgctgctgt tcatcttgtt cgtgtttgtt ttaggacacc agaagaagga atggaagttg
    10681 ccaagctggc taaatctgtt tacttttcta aatggaatgc tagggaattt ccttttttta
    10741 tttagcagtt tttatgttgg catactgtac ggattagaaa aactttcgat ttttatgtat
    10801 ttgccttatg cagcaggctt gattgcagca aaaatagtcg gaccatggat tttgaaacgt
    10861 ttaacatggt ctccattggc tatgcaattg cttggactga gttcttcttt attggtcctc
    10921 ttgattccaa atggttttgc gataggtgtc tttctgctta gtttctggca cgtagtaaca
    10981 ggcagctggc taaataagga atattatcaa actgatacag ctattccaat ggatcgccgg
    11041 atcgttgtca agtatactac tcaaaataaa gggagtgtga tccaccagtt tttattgatg
    11101 acgcttttgt ttgttttagc aaaagaaatg ggagagccaa tccatctatt attgatcacg
    11161 ctgaaccatg aatcagtgcc gatagaaagt gtacaactgc ttatcttggc aaaatggatc
    11221 aatgttggtc ttttactgag cggattagta actgtttatc atttatggaa gaaggcgaaa
    11281 aagaatgagg ctattcattg atggagatgg ttctccagta aaagaagatg tcataaaatt
    11341 aggagaatcc tatggtcttg acgtggttat cgtgaccagt gtggaccatt atacgaccaa
    11401 agaatacgct aatcatgtcc aaatcgtcta cgtagaccgc ggagctgata gtgcggatta
    11461 taaaatcgtc ggtatggtta agaagaatga ctttttgatc acgcaagatt atggtttagc
    11521 ttcacttgta ttgccgaagg gtgtgcgagt gttccatcac tctggaaaag aatttgacga
    11581 agcgaatatc ggcacgcttc tagaacaacg ttatcatagt ggaaagttaa gaaaagctgg
    11641 cataaggaca aagggtccaa aaccctttac ggagaaagat catatgtctt ttcgtcaagc
    11701 gttaaaaaag gcaattgact aaggtttacc aagtacagaa gatagtaggt aacgaaggta
    11761 ttttatttat gctcgaatac atcaaaaagg gaacagggaa acaggataaa tgtgttccat
    11821 gttccctttt tgattgttct tattactggg tgcagaacag ctttttcaca ttttttattc
    11881 tcggtgtttc agaagtagcc tcttcgaaaa ataagccgaa ttttcacaaa aatttgagaa
    11941 acaatttatg taaaacccct tcttatttct tgaagctgat tctttctgtc acaacctttt
    12001 tctacttttt cctcttctta tacatttgga tcaatgcatc gattgccaaa aaaataatga
    12061 cgaatgcggc aatagcatac gtaaatatcc ctaataagga aacgacctct gcgttttcta
    12121 caggtgctgc ttggacaagc agggaggacc ctacgatcaa tgcagctaaa atcataccga
    12181 aaacaagccg attggtcata ctttcgatcc tgctcaataa tttgtcttgc tttttcaatt
    12241 ccaaattgac ttttccttgt cccgaagtcc acatttctaa tagatggtgg agctgttgag
    12301 ggatcttagg cgcagccttc atcccttcca ataagtctaa tccgctttgt ttcaatgtat
    12361 cttctacatc cagatgagtg agaaaataat gttgagcaaa aggcgaagcg acttccatga
    12421 gggaaacttc aggatcgagg acgcggatca ccccttctaa tgtcccaaat gctttcagca
    12481 aaagcgtgat atcacgatcg aactgcagat tattttgatg acaaatcgtc acgacatgtg
    12541 tgaatacttc ttgcaagttg atttcatcaa taggagaatc ataatactgt tcaagaaaac
    12601 ttgtcagttc ttggtggaac cgactttcat caaacgagcc ttcttgctga caaagtcgaa
    12661 ggaccgcttt ttcgattcga taagcatctt tcgtatacag cgctaaaacg gcttgtgtga
    12721 gtttttgcct aagtcctgca gaaagatgcc ccatcatacc aaaatcgatg tagttgatgg
    12781 tatatggcgc aacagggtct tctgcagaag tggaggctcg aaaagcaaag gaaccgaact
    12841 ctttttcatg gactgtttca gtttttcggc tagcttgtgt ctgttcttct tttgttagta
    12901 catggaatag cagattccct ggatgaggat cagcatggaa aaaaccatca tcaaacacct
    12961 gtttcataaa gttctcaact aaaagctttg caacttcttg tttcagctgc ttgttttgaa
    13021 ttccagtgat aaaagtttca gttttgtttt cggcgttcat taaatgattc aagtttttac
    13081 cagacatttc ttccatcacg atcacttttt tcgaacaaaa agcgtcataa atcttggggc
    13141 tgcggatctc tttccagcca ttgttctttt gatagaattg ttcgccattt tgactttctt
    13201 ttaagaagtc catttcattg atcaaggaac gttttacttc ttggagtacc ccttttagat
    13261 caactacgct agtttcagga atgtatttga tcaaagggat cgctcgttca aatagttgaa
    13321 gatcaagtcg gatctcttcg gcgatgttag ggtgttggat cttaacgact accgcgtcac
    13381 cgttcttcag cttggcacga tgagcctgcc ccatagaagc agaggcaaaa ggcgattttg
    13441 aaaaatcatc gaatatatct gataatgaaa gagaaagttc tgtttcgatc gtctttttga
    13501 ccgttgaaaa tgtgtcgcta ggtacactat cttgtaatgt tttgaatgtt tgagtgaagg
    13561 cagaagaaag caaatcttct cgaacagaaa gcatctgtcc tatcttgatg aaggtggggc
    13621 ctagttcttc aaaggctttt tttacctgtt cgggattttt ttgctgtaca aagtttggca
    13681 ctacattgta attgatgaac actcgaatga tttctgccaa gcggctgcgt ggatttgttt
    13741 gcgccatcat catccctcct tttctttatc ataacttttt ataaaaaacg ggacaaataa
    13801 tctagctgac aaaaaataaa tgacgctcgt tcttttagaa gctggctcgt ttttttataa
    13861 ataagttatt cgtttatttt tgttttgtgc taaaatatga gagtaaatca ctgaacgatt
    13921 tagaatacag gaggacaatc ttttgaagat tactttacta tatggcggac gcagcgcaga
    13981 gcatgaagtg tccattcttt ccgcattttc agttttaaat gccatttatt ataattatta
    14041 ccaagttcaa ctcgtattta ttacaaaaga aggacaatgg gtcaaaggtc cattactaac
    14101 agaaaaacct gccagcaaag atgtcttgca tctttcatgg gacccaagtg gacagacaga
    14161 ggaaggcttt acaggaaaag tgatcaatcc gggcgaaatc aaagaagaag gagccatcgt
    14221 ttttccagtt ttacatgggc caaacgggga agatggaacg atccaaggct tcttagagac
    14281 attgaatatg ccttatgtcg gcgcaggcgt attgaccagt gcatgtgcca tggataaaat
    14341 catgaccaag tatattttac aagctgctgg tgtgccgcaa gttccttatg taccagtact
    14401 taagaatcaa tggaaagaaa atcctaaaaa agtatttgat caatgtgaag gttctttgct
    14461 ttatccgatg tttgtcaaac cggcgaatat gggttctagt gtcggcatta caaaagcaga
    14521 aaaccgagaa gagctgcaaa atgctttagc aacagcctat cagtatgatt ctcgagcaat
    14581 cgttgaacaa ggaattgaag cgcgcgaaat cgaagttgct gtattaggaa atgaagacgt
    14641 tcggacgact ttgcctggtg aagtcgtaaa agacgtagca ttctatgatt atgaagcaaa
    14701 atatatcaat aataaaatcg aaatgcagat tccagccgaa gtgccagaag aagtttatca
    14761 aaaagcgcaa gagtacgcga agttagctta cacgatgtta ggtggaagcg gattgagccg
    14821 gtgcgatttc tttttgacaa ataaaaatga attattcctg aatgaattaa actctatgcc
    14881 aggatttacg gagttcagta tgtacccact cttatgggaa aatatgggct tgaaatacgg
    14941 tgatttgatt gaagaactga tccagttagg aatgaatcga taccatcagc gtcaatcttt
    15001 ttttgaaaaa aatgaataaa gagaaataaa gaagaggctg gagtgattgc gtaaccgcgt
    15061 tcattctagc ttctaaatac gaatagagaa atttacttgg aagtgagagt catgcgaacc
    15121 cgccgtttag gccaaacgct tttttcacaa ccttttttag gagagatctc aatgaatcta
    15181 acaatacaag aaattgcaca agccgtgggc agcgaatgct ctgcactgac aaagattacc
    15241 aatgtcgaat ttgacagccg gaagataacc gaaggaagtt tattcgttcc tcttgctggt
    15301 acgcgagatg gacatgaatt tatccagcaa gcgatcacaa acggagcatc tgcttcattt
    15361 tggagcttgc ctgttgaaca agctccaaaa aattttccag ttattcctgt agcagatcct
    15421 ttgaaagcca tgcagcaatt ggcaacctat tacttggaaa aagtaggcgc agaagtcgtt
    15481 gcaattacag gaagcaatgg taaaaccacc acgaaagatc taaccgcgtc tgtcttgtct
    15541 gaaaaataca aaacatataa gacgcaagga aattacaaca ataatattgg tcttccttac
    15601 acgattttgc acatgcccca agatacagaa aagatcgttt tggaaatggg catggatcac
    15661 gccaatgaaa tcacggaact ttcacttatg gctcaaccaa aagctgcagc aatcacaatg
    15721 atcggtgaag cacacataga aaatcttggg tcgcgagaag gaatcgctaa agccaaaatg
    15781 gaaatcactg acggacttgt atcagacgga ttactcgttg tgcccgcaga tgaaccgttg
    15841 ctgacacctt taacggcaac attagtacaa acagtcacaa cattcggtat tggaaatggc
    15901 gatgttcatg cggaagtgac agcaaaagga aaagcacaaa ccgattttgt agtagaagga
    15961 gaaacattta cgatcccgct gcctggaagt tataatgtaa cgaatgcttt gattgcgtat
    16021 acgatcgggc gcttctttgg actgtccata cctgagatac gccaagggct cgcccatgtt
    16081 tctatcactc aaaacagaac cgaatggcta acggcaggaa atggtgcagc tattttgagc
    16141 gatgtctaca atgcaaatcc aacagccatg ggactggtat tagatacttt taaaaatctg
    16201 ccaacaaaag gacgcagaat cgctgtgtta gcagatatgc tggaattagg gaaagagtct
    16261 ttaaatatgc atgcgcaaat gagtgaacat atcgatcctg agaaattcgc catgattttc
    16321 ctttatgggg aacaaatgca tgcactaaaa gaagaattga accaaaaata cccccaacta
    16381 cctgtgttct attttgaaaa agaaaagaat aaattgattg atgctatcaa acaaaatctt
    16441 gaagcaacag atagtattat gctaaaaggc agcaacagca tgggcttatt tgaagtggta
    16501 gaacaacttc agcaaatgaa ataaaaatct gtaaacttgc agaaaacgaa aaactatgtt
    16561 agaataacct attgccgaaa gatgaagaga aattggactg gttttgccat agaaacaact
    16621 tgttgaagcc agatgtcata ataatgataa agctgaacgc gacccgtttg gcttttctaa
    16681 tgcctaaact tcgcaaagtg atttttcggt ggtgccacag gggctaagaa tggataaaaa
    16741 cgtgcgtggc cactgtcaaa aagaaacaat acactttgac cgaacaaatc gacggggtag
    16801 caaaaaagct acatcctcag ttaacaacca tataggagga ttcatttgaa atttaaagaa
    16861 ctagaattat caccagaatt gttgaaatca gtcgaacgcg ctggttttga agaagcaacg
    16921 cctatccaag cagaaacgat tccactagca ttagctggaa aagacgttat tggacaagca
    16981 cagacaggta cagggaaaac agctgcattt ggattaccaa tgttagaaaa aatcgatcca
    17041 gatcgtcacg aattacaagg tttagttatc gcaccaacac gtgaattagc tatccaaaca
    17101 caagaagaat tatatcgttt agggcgcgac aaaaaagtac gtgtacaagc cgtttacggc
    17161 ggagcagata ttggccgtca gatccgtggc ttgaaagatc gtccacatat cgttgtgggt
    17221 acaccaggac gtatgcttga ccacattaac cgccacacat tgaaacttgg aaccgttcaa
    17281 acattagttt tggatgaagc agatgaaatg ttgaatatgg gcttcttgga agatatcgaa
    17341 aaaatcattt cacaagttcc tgatcaaaga caaacattac ttttctcagc aacgatgccg
    17401 ccagctatca agaatattgg cgttaaattc atgaaaaatc cacatcatgt gaagatcaaa
    17461 gcgaaagaaa tgacagctga cttgatcgat caatactatg tccgcgcaaa agaatacgaa
    17521 aaattcgata tcatgactcg cttgtttgat gttcaaacac ctgaattaac aatcgtcttc
    17581 ggacgtacaa aacgccgtgt agacgaattg gcacgcggat tagaagcacg cggttaccgt
    17641 gcagaaggaa tccatggcga tttgtctcaa caaaaacgaa tgagcgtatt gcgttcattt
    17701 aagagcggtc atttagatat ccttgtagcg acagacgttg ctgcacgtgg attagatatt
    17761 tcaggtgtca ctcatgtgta caactatgat atccctcaag atccagaaag ctacgttcac
    17821 cgtatcggac gtacaggccg tgccggaaaa ggcggtatgt ctgtgacttt cgttacacca
    17881 aatgaaatgg actacttgca tgtgattgaa aacttgacga aaaaacggat gacgacttta
    17941 cgtccaccaa ccgaaaaaga agcattcaaa ggtcaattag gtgctgcagt tgaacaaatc
    18001 gaaacaaaac ttgcagaaaa tggtctagat aaatacctgc aaacagcaga taaattatta
    18061 gaagaatatt ctgcacaaga tttagttgca cttttgctaa aaacgacagc aaaagatcca
    18121 gctgatgcag tgccagtgaa gatcacacca gaacgcccat tgccaatgca gaaaaaagga
    18181 tacaataaaa atggcaaacg tggtggcgga aacaaccgta atcgtcgtga cggcggtaat
    18241 tatcgtggaa acaaatcaaa aggcggttat agcaaaaata ataacagcca aaaagatggc
    18301 ggtaaacgtc acaatgataa aaaacgcgga tttgtaatcc gttcaaataa cgattaaaca
    18361 taaaatgaaa gtttctcttt tttaagagaa actttcattt tt
//
LOCUS       DAZKNM010000046        17336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000046 DAZKNM010000000
VERSION     DAZKNM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.046"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            255..944
                     /locus_tag="ACG8M9_001238"
     CDS             255..944
                     /locus_tag="ACG8M9_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0378024.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            931..2139
                     /locus_tag="ACG8M9_001239"
     CDS             931..2139
                     /locus_tag="ACG8M9_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0378025.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2433..3704
                     /gene="serS"
                     /locus_tag="ACG8M9_001240"
     CDS             2433..3704
                     /gene="serS"
                     /locus_tag="ACG8M9_001240"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0378026.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4253..5737)
                     /gene="guaB"
                     /locus_tag="ACG8M9_001241"
     CDS             complement(4253..5737)
                     /gene="guaB"
                     /locus_tag="ACG8M9_001241"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0378027.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6242..6937)
                     /locus_tag="ACG8M9_001242"
     CDS             complement(6242..6937)
                     /locus_tag="ACG8M9_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0378028.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7027..8127)
                     /gene="ychF"
                     /locus_tag="ACG8M9_001243"
     CDS             complement(7027..8127)
                     /gene="ychF"
                     /locus_tag="ACG8M9_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0378029.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8136..8327)
                     /locus_tag="ACG8M9_001244"
     CDS             complement(8136..8327)
                     /locus_tag="ACG8M9_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0378030.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8348..9238)
                     /locus_tag="ACG8M9_001245"
     CDS             complement(8348..9238)
                     /locus_tag="ACG8M9_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0378031.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9225..9992)
                     /locus_tag="ACG8M9_001246"
     CDS             complement(9225..9992)
                     /locus_tag="ACG8M9_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0378032.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10004..10717)
                     /gene="rsmG"
                     /locus_tag="ACG8M9_001247"
     CDS             complement(10004..10717)
                     /gene="rsmG"
                     /locus_tag="ACG8M9_001247"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0378033.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10949..12850)
                     /gene="mnmG"
                     /locus_tag="ACG8M9_001248"
     CDS             complement(10949..12850)
                     /gene="mnmG"
                     /locus_tag="ACG8M9_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0378034.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12867..14264)
                     /gene="mnmE"
                     /locus_tag="ACG8M9_001249"
     CDS             complement(12867..14264)
                     /gene="mnmE"
                     /locus_tag="ACG8M9_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0378035.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14563..14946
                     /locus_tag="ACG8M9_001250"
     CDS             14563..14946
                     /locus_tag="ACG8M9_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378036.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15114..15872)
                     /gene="jag"
                     /locus_tag="ACG8M9_001251"
     CDS             complement(15114..15872)
                     /gene="jag"
                     /locus_tag="ACG8M9_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0378037.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15890..16708)
                     /locus_tag="ACG8M9_001252"
     CDS             complement(15890..16708)
                     /locus_tag="ACG8M9_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0378038.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16705..17061)
                     /gene="rnpA"
                     /locus_tag="ACG8M9_001253"
     CDS             complement(16705..17061)
                     /gene="rnpA"
                     /locus_tag="ACG8M9_001253"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0378039.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17179..17313)
                     /gene="rpmH"
                     /locus_tag="ACG8M9_001254"
     CDS             complement(17179..17313)
                     /gene="rpmH"
                     /locus_tag="ACG8M9_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0378040.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
BASE COUNT         4811 a         3624 c         3183 g         5718 t
ORIGIN      
        1 taagaaaatg cttatttatc aacatttcta gcttacatga tcattggtta gttctggtcc
       61 tttttcgttc gtctttataa ctccgagaga cgtaaaatcc ttttgttttc agtttaatgt
      121 tcatgttctt gtcttattca caccaactag atgtctcacc ttctaaaatt ttccttaaca
      181 aagtgctgct tttatctatt tctgcttttt tttgctagaa taatagtaac aatatgactc
      241 tggaggattt atttatgaaa attttgattg ctgacgatga taaagaaatc gttgaattat
      301 taagtatcta tgttcacaat gaggggtatg aagcagtaaa ggcttatgat gggaaagaag
      361 cattatctaa aatcagaaca atccctgata ttgaattact tattttagat atcatgatgc
      421 ctgagatgga tgggatgcaa gtagtgaaag agctacgaaa agaatcccaa ataccaatca
      481 ttatgctgac agcaaaaact actgatatgg ataagatcaa aggactggtc gctggtgcag
      541 atgattatgt gaccaagcca tttaatccgt tagaagtaat ggctagagta aagtctttat
      601 tacggcgtac aaaaatgcag attgccccag atcagcctga tgtattagaa gtaaatgcat
      661 taatcatcaa taaagactct catgaagtta aaacaatcga tgggaaagaa atccagttaa
      721 cagctttgga attcggtatc ctctacttgc ttgctagtca tcctaatcga gtatttagcg
      781 cggatgaaat ttttgagcgt gtttggaaac aagaaagtat tgtctctgct aaaactgtca
      841 tggttcacgt tagtcattta agagataaga ttgaagaagc aactggtggt gaaaaagtca
      901 ttcaaaccgt ttggggagtg ggctataaaa ttgacgcaag atagtaaacg agcaactgaa
      961 aaacgccgtc ggatcaattt gacttcaaaa gaaatcagcg aattgttagc ggaaggaatc
     1021 atcacgatta tccttttgct tctattaaat gtttctattc tggttgttat cagttcggtg
     1081 atcaacagtt caccttcttt gacgaatgcg atttgggatt caaagaatat ttttgcagaa
     1141 cgattgaaca ctgatttgtt ctggaacggc cggaatttta ttattccctt tttctttcta
     1201 ttagatattg gtgttttgta ttggcgcttg attcgaagat atcgccaaat gcaattgcgg
     1261 catatcatca gcgaacttca ctacatcgca aacggaaatt atgatcatcg tatccctttt
     1321 gaactgagtg gtgacttaag ccgagtggta acaagtatca atggattggt ggatagtacc
     1381 gtagcagcga ttgaagacga acgtaaaatc gaaaaatcaa aagatgaatt gatcacgaat
     1441 gtcagtcatg atatccgtac cccactcacg tccatcattg gatacctagg actgatagaa
     1501 gatggccagt atcactcaga agaagatctt ttaaagtata cgcatactgc ttatatcaaa
     1561 gcaaagcaga tgaaatcttt agtggacgac ttatttgaat acactaaggt acgccagcct
     1621 gctgtcccgg tgaatttttc tgcctttgac atgatccagt tgatcgaaca gttagcagct
     1681 gattttgagt tggaagcttc taaaaaaaat atccaaatac ttgttcaatc aaaagtagac
     1741 tcccttatca tggatggcga taccgaaaaa cttgtacgtg tcttcaataa tcttttgacg
     1801 aatgcgttga aatacggaaa aggagcaacg aagatcgtaa tcgaagtaga acgtatcggt
     1861 tcagaagttg ttgcaacagt caaaaataac ggagcaatga tcccacaaca ggcaatcgat
     1921 aatctattcg accgttttta tcgtgtagaa gaatctcgtt cacaagcaac tggaggaact
     1981 ggacttggtt tagcaatcgc tcaaagcatt gtggctttgc acggcggata catttatgca
     2041 aaatcagata agcaatggac ttctttcatc atgcaccttc caatcaagaa aaatgaaaaa
     2101 ttacctattg atacacagga agttattgac gaaagctgaa atattcgcta ttcttattac
     2161 aattattaga ataaatgaaa aacacagaac ccagtacact ttattctttt tcagagagct
     2221 gatggcaggt gtaaatcagc aaaggataat ttggaagttc cagttcgttt acgtgccctt
     2281 tcgaaataag aaaggtcggt tcaacagccg ttatgtgttc aacaagtgca gctgacgctg
     2341 aacttgggtg gtaccgcgaa tttcagactg attcgtccca agaataatgg gatggatcag
     2401 tcttttttta ttttcaaaaa ggaggaaatc atatgttaga tgtaaaaatg atgcgacaaa
     2461 atattgaaga agtacaagaa aaattgatga ctcggggcgt aaaaaaagaa acattagctg
     2521 aattcatgga actagatgaa aatcgtcgta agctactagt caaaagtgaa gaattaaaaa
     2581 aatatcgaaa tgatgtttct gctgagatcg cccaattgaa gcgtgcaaaa gaagatgctt
     2641 ctgctaaaat cgctgaaatg aaagaagtcg gtggcaacat caaagcattg gatactgaaa
     2701 ttgctgaagt ggatgaaaaa ttgaaacaaa ttgcaactac tttgccaaac cttccccatg
     2761 attctgtgcc agttggaaca gatgaagagg acaatgtaga aatccgtcgt tggagcgaac
     2821 cacgtacatt tgcttttgaa ccaaaaccac actgggaaat tgcagaaaat cttgatattt
     2881 tagattttga acgtggcgca aaagtagccg gtagccgttt tgtctattac aaaggattag
     2941 gtgctcgatt agaacgtgct gtctacaact tcatgcttga tcagcatatt tacgaacacg
     3001 gctatactga aatgatcact ccttatatcg tcaacagtaa agcgatgttt ggtacgggac
     3061 aatttccaaa attcaaagaa gatgttttcc aattggagaa tactgatcta acattaatcc
     3121 caacagctga agtaccatta actaactatt ataatgatga aattttagat ggtgcagatt
     3181 tacctattta tttcactgca ctaagccctg cttttcgttc tgaagccgga agtgctggtc
     3241 gggatacacg aggactgatt cgtcttcacc aattcaataa agtagaaatg gtcaaatttt
     3301 cggatgtaga gcattcttac gaagaattgg aaaaaatgac agctgatgca gaagatatct
     3361 tgaaaaaatt gcatttgcct tatcgtgtga tggccttatc aactggagac atgggctttt
     3421 ctgcagctaa aacttatgac ttagaagtat ggatacctgc acaagatact tatcgtgaaa
     3481 tcagttcttg ttctaactgt gaagatttcc aagcacgccg cgcaatgatc cgttatcgcg
     3541 ataatgatgg aaaaatccac tatactcata ctctgaatgg ttcaggactt gcagttggcc
     3601 gtacagtagc tgccattttg gaaaactacc aaaatgaaga tggttctgtc acagttccag
     3661 aagcgctcgt tccttatatg gggaatctga aagttattaa ataaagaggc tgtgaaaaag
     3721 ccgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
     3781 aattggactt aataccgaga aactggcttt tgaacaccat ttatcgagag gttgtgaaat
     3841 cagcgaatta acctgagtat tagagaaaaa aatggaaaaa tagtccctca tattttaccg
     3901 tttgttgaac taataccgag ggtcagctga ttgaacaccg tttagtggag aggtcgtaag
     3961 cctacccccc agctcctagt tacaaggaca aattcaaaaa acagctcttc atattttatg
     4021 aatgttgtac ttgtgattcg gaggtggcaa gggaacactg tttattcaaa aagaggctgg
     4081 gacagaagcg tttaactcca agaaataaga agaaattcac gaaaattgct tttcaaattt
     4141 ttgtgaattt cttcttattt ccgaaggagt tgcttctact tccgccgttt atacgtgttt
     4201 agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatgt ttttaagatt
     4261 caactgaata gtttggtgct tcttttgtga tttgtacatc atgagggtgt gattctttta
     4321 atccactgcc gctcatttgg ataaattgcg cttcatcacg taattgctgt agattggctg
     4381 ccccaacata acccattcct gaacgcaaac cgccgatcat ttggaaaata atatctgcaa
     4441 cacttccttt ataagcaacc cgaccttcga ttccttctgg tactaattta tttgcttcat
     4501 tgacactacc ttggaagtaa cggtcttttg aacctttttc catcgcgcct aatgagccca
     4561 tacctcggta tgttttgaat cgacgtcctt ggaagatttc gaattcacct ggtgattcat
     4621 ctgtccctgc caacatactt cctagcatca ctgcatgtcc gccggcagct aatgctttga
     4681 cgatgtcacc agaatatttg atccctccgt ctgcaataat cgctttgcca tattgacgag
     4741 caactgatgc tgcatcataa atagctgtga gctgaggtac acctacaccg gctaccacac
     4801 gtgtcgtaca aatcgatcct ggtccgatac caacttttac gacatctaca cctgcatcat
     4861 ataaagcttt tgtcgcttca gccgtcgcaa cgtttcctgc aatcaatgtt gcatctgcaa
     4921 aagttgaacg gatttcttga atttttcgga tcacacctgc actatgtcca tgagctgtat
     4981 cgatgatgat tgcatctgcc cctgcttcta ataaagcatt cgcacgctca aatgtatcgc
     5041 ttgttacacc aacggctgct gctacaagca atcgaccatg ttcatctttt gctgcatttg
     5101 gaaattcgat cactttctcg atgtctttga tcgtgatcaa tccgcttaaa cgaccttcgt
     5161 tatctacgat tggtaatttt tcgattttat gtttttgcaa aatcttttct gcatctttta
     5221 gagaggtacc tactggggca gtcactaagt tgtcttttgt catcacatca gcaatcggca
     5281 tgtgataatc tgttacaaaa cgcatgtcgc ggtttgtaat tatccctacc aattttctgt
     5341 tttcaagtgt ttcaacgatt ggcacaccac tgatacgata tttgctcatc aattcttctg
     5401 cgtctgctac aagatgctgt ggcgttaaga agaacggatc aatgattacg ccactttcag
     5461 aacgtttgac ttttcttact tcatccgctt gctggctgat cgtcatgttt ttatggatca
     5521 cacctagtcc gccttgtctt gccatagcaa tggccatctt gctgtcggta acagtatcca
     5581 tacttgcact gatgattgga atattcaact taatattttt agctaattgt acgctcatat
     5641 ccacttcgtt tggtaacacg tgactttctg ctggtatcaa taagacatca tcaaaagtaa
     5701 atcctttttt cgtgaacttt gtttcccagt tggacatttt tagtaccact cctctaattt
     5761 agtttatcta ttaaaataat tgattttttc atttaagtca atgatctact tggaatcaag
     5821 ttagctttct catagaatac caatataaag cgtttacatc tgtgtgtatt aaaagagatt
     5881 gtaaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     5941 ttttgaattg gttgggttta ttaccgcaga tgcaaacttt tgaacaccgt ttagtggaga
     6001 ggctgtgaaa aagctgctta gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc
     6061 acatttttgc ataaattgga cttaataccg agaaactggc ttttgaacac cgtttatcgt
     6121 aaaaagggac tgtgacaaga cttttgtcac agtcccttac tattatttaa ctatggataa
     6181 acaaaatgag gttctttact acccctagat atctagcaaa atagctgatc tttttgctag
     6241 tttatcttct tgggacggat aaacttccaa taatcccgta ttttttcttc aagtagtaac
     6301 ggattgctaa agcagttgcc ccgataatga taactactac tggatctaat actggattga
     6361 taactggtgg caatagtgtt gtcgcagaaa acagtacgac ccaaagaagt gtcgttccac
     6421 ctaggataag caatgtctta cccattcctg gtttcttgct tcgatctgct ccgggatatt
     6481 catattgata gatgtatttg tacatcatat aaaagaccca gccaccgatt gcagaaccta
     6541 gaacgagtgt agaaaagcca taagctggcg ttgttccacg tgaaaacaat ccggtaatcg
     6601 caatcatgat acttaataga ccaagcaaca gcaacgtatt atctaaccac atcaataaag
     6661 gggtggatga attttttact tctgctggct tattgatgat tgcttccgtt ctttcagaga
     6721 ctgtcccaaa caattgacgg gctgttcttc cgcctttttg ttctgcaact aaagtaggca
     6781 gaatgtcatg aagggctttt actttttctt cttcagacaa attcgctgct tctaaagatt
     6841 ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa ttttgtttct aaactattgt
     6901 tttcttttac gattgctcgt aattgttctg gttccattat ctaatagtcc tccttagccg
     6961 ttccttttct caacggtcgt cacaaaagac attctaccat attctacagc tagtttgata
     7021 gcccttttac acattaaagc ggaataacat tacgtcgccg tcttgtacga cataatcttt
     7081 tccttctaat cgaacacgtc cagcttcttt tgctgcatgc atgtttccgt aatgatccaa
     7141 gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt tcgaaatctg aatggatgat
     7201 tcctgctgct tgtggtgctt tgattccttt tttgaatgtc caagcacgta cttcttgttc
     7261 cccagcagta aagtaagtag ctaagcctaa caaatcataa gcagcacgga tcaactgatc
     7321 caaacctgat tcttcgattc ctaaagcttc taaaaattcc gctttgtctt cttcatctaa
     7381 ttctgcgatt tcttcttctg cacgagcact gaccacgatc acttctgcat tttcttctgc
     7441 agcaaagttg cgcacttctt gtacataatg attcgcgtct gcatctgcta cgtcatcttc
     7501 tgagacattg gctacataaa gaattggttt tgtcgtcaat aaaaatagtg atttaacgat
     7561 tttttgttct tcttcagtaa attcgatcgt tcgggcagat tttccttctt ccagtacagg
     7621 tttgatttta tccagtacag ctagttctgc cactgcatct ttatctttgg ttttagcaac
     7681 ttttgctaca cgagtatagc gtttgttgat agattctaag tctgctaaca ctagttccaa
     7741 gttgatcgta tcaatatctt ctagtggatc aacacgtcct tctacgtgag taatattatc
     7801 atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact tggcggatat ggcttaagaa
     7861 ttgatttccc aaaccttctc ctttacttgc gccttttacg attccagcaa tatctgtaaa
     7921 ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt tctgttaagc gttgcaaacg
     7981 ccagtcaggt acttctacca tccctacgtt ggggtcgatc gtcgcaaagg gatagtttgc
     8041 tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt gattttccta cgttgggcaa
     8101 cccaacaatt ccagcagtca atgccataaa tttattcact ctttctctgc atttttttca
     8161 attacttttt ttaatttctt ttcaaaatct cgacgggtca tcatgacgat atgtccgcaa
     8221 ttcatacatc tgattttgat atctgcgccc attcgaatga cttcccagcg attcgtctgg
     8281 caagcgtgag gttttttcat ttcaacgata tcgtgcaagt cgtacataaa aatagcctcc
     8341 tttttgttta ttcttcatca aattgaatat tcaagatatc aagaattcga gtcaaatctt
     8401 tttgagaaag atattcaatc tcgatcttac ctttcccatt tttttcttgg atagctacac
     8461 ttgtaccaaa tttatccatc aaacgatctt cgctttctcg gatataatat ggtttttcct
     8521 ttgctagcct tgggattttt ttcttttctt ttccatcatt ttgatttaac tcagaaacaa
     8581 gctgttccag ttggcggaca gttaaatttt cagttactgc acgattggct aatttcaaga
     8641 tcaatgtttt attttttaaa cctaacagcg tacgggcttg tcccatagat aaacgctgat
     8701 cttgtaccat ttccttgaca agatccggta aagaaagcaa gcgcaaataa ttggcgatat
     8761 aaggtctgct ctttcctaaa cgactagcta cttctgcttg tgttagtttc aggtttttca
     8821 tcagcatctc atatgcttct gcttcttcta acggattcag atcttctcgc tgcaagtttt
     8881 ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc tctaacgata gcagggatcg
     8941 tttctttttc agctagtttg gacgctcgaa aacgtcgctc ccctgcaata atctcatatc
     9001 cttttactgc ggaacggcgt acgatgatgg gttgaaagac acctgattgt tcaatagaat
     9061 tagctagttc ctgcaaagat acttcttcaa atgttttacg gggctgatac ggatttggtc
     9121 gcagctcatt caatggtagc tggacaacgg tatcatcttt tacatccacc gtctctaagt
     9181 tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa tcctttactt ttcttcacga
     9241 cttaccactt cctttgccaa tgcttggtag acttcagctc ctcgagaacg tggatcgtag
     9301 tcgatgatcg gttttccatg actaggtgct tctgataaac gaatatttct tggaatgatc
     9361 gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt ctacgacttc atttcccaga
     9421 tttgttcgtg catcatacat tgtcagtaag actccctcga tctctaattc tggattaaag
     9481 tgtttttgga ctaaacgaac cgtattcagt aattggctta atccttccaa tgcgtaatac
     9541 tcacactgaa caggtatcaa aatcgaatca cttgctgtaa atgaattgat tgtcagatgg
     9601 ccaagagaag gcgggcaatc gatcaaaata taatcatact gactgcttac ttctgctaaa
     9661 gatcctttta atcttgattc tcttgccatc attgacgtca attcgatctc tgctcctgct
     9721 aattggagag tcgcaggcac aatactcaaa ttttcgtgtt cagtgatcaa agttgcctca
     9781 tcaattggca attcattgac tagaacatca tagatgtctc ttgtcacatc aggttttctg
     9841 atcccgacac cactggtagc atttccttgt gcatccatat ccactagcaa tacttttttg
     9901 ccaaggctag caagacaagc acctagattc accgtcgtcg ttgttttgcc gacgccacct
     9961 ttttggttcg caacagatat tatttgtgcc atctcttctc ctcctatttg attggctgct
    10021 tattaggcag tccgggcttt cttgggtatt tctttggggt tggtttcttt ttttgaataa
    10081 cgatgatatg ccgttgatca gagacattcg gcaaggtaac cgcctcttct ttgaggaatt
    10141 taccacctaa taaagcaatc gcttgttttg cttctgtcaa ttcctcttca ctttttgctg
    10201 ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg caaacacaat tccgctaaca
    10261 cattcaaacg agcaaccgca cgagcagtga caaggtcaaa tgatgcacga aagtgaggat
    10321 tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc atcgattcct aataatcggg
    10381 ttaactcaga taagaaggtt attcgtttat tcagagaatc aacgatcgtt atctgcagtt
    10441 ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc ccccgaacca acatcgcata
    10501 aagataaagg ttgcgtaaag tctacttcaa atgctaatgt aagtgaatcg taaaaatgct
    10561 tcagatatac gtctttttca tccgtaatag ctgtaaggtt catcttttcg ttccattcga
    10621 ctaatgtccg ataataaata gcgaattgat tcatttgatg atcggtaagc tctatccctt
    10681 tttctgccaa ggattggcga aattcttctg gtatcataaa tattcctttc attgtgaaac
    10741 agcaattgtt tcacagattc tctccctact ttaacgcaaa atctctagac atgcaatcct
    10801 tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc gatagacttt ttctttctag
    10861 tatgactcat aaaatataca cagaagagag aactagcttt gagcaatttc tgcaaaaact
    10921 agttctctct tctatttatt attacgtatt acgctggttc actgactttc gcaatcttcc
    10981 cttgttcgat atagaccatt aggatactta ggtctgcagg gtttacgcca ctgattcggc
    11041 tagcttgggc aattgtttct ggttggattt tctgcaattt ttgttttgct tctgttgcta
    11101 atccgttgat tgctgcgtag tcgatgtttt ctggaatctt tttcgcttcc atgcgtttta
    11161 atttttctac tttttccatg gcttttttga tgtagccttc atatttgatt tggatttcta
    11221 cttgttcgat ttcttttcgt gtcaattctt cattttctgg aataaaccgt agaagttctg
    11281 cataagtaat ctcaggacgt cttaaaaagt cgcttgctag tacaccatct tttaatggtg
    11341 ccgcattaat cgttgctaaa aagtcttgga cttcttctgt tggtttgatt cgtacattca
    11401 tcaaacgttt gatttcttgt tcaactgctt cttttttctt caaataattc ttgtattgat
    11461 cttcttttac taagcctaca cggtgaccca tttctgttaa tcgcaaatct gcattatcat
    11521 gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa acgataaggt tcgttcgttc
    11581 cttttgttac caaatcatcg atcatgacac cgatgtaacc gtcacttcgt ttgatcacta
    11641 aaggttcttt tccctgaact ttcaaagctg cattgatacc ggcaatcagc ccttgtccag
    11701 ccgcttcttc ataaccgctt gtaccatttg tttgtccagc tgtataaaga ttttcaacga
    11761 ctttagtttc taatgtcgga cggagctggt gtggctctac tacgtcatat tcgatagcat
    11821 atcctgtacg catcatttct actttttcta atccagcaat agaatgaagc atgttgactt
    11881 gaacatcctc tggtaaagaa gtagataaac cttgaacata gacttcttcc gtatttaagc
    11941 cttctggttc caaaaataat tgatgacgtg gtttgtctgc aaaacgcacg atcttatctt
    12001 cgatggatgg acaatagcga gcgcctaccc cttcaacgat ccctgtaaac attggggcac
    12061 gatgtaagtt gtctcgaata atttggtgag tcgtctcacc tgtataggtc agccagcaag
    12121 attcctgatc tagtttatag gcactatctg gtgtgctgaa gctaaaatga ttcggctctt
    12181 tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt acttgatttt actcgaggcg
    12241 gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc ttttagatga ttcgctagtc
    12301 caatagaagg ttgagagtta ttcggacctg atgaatattt caattcaccg ataatgattt
    12361 ctccgcgaag tgctgttccg gctgtaataa caactgcttt ggcccggtag ctcgctcctg
    12421 tggacgtcac tactccatga caaacaccat cttcgacgat cagttcttcg acgattcctt
    12481 gtcttagcgt caaatttggt tctttttcga tcgtatgttt catttctgtt gaataagcat
    12541 gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc ttttcctgtg ttcaacatac
    12601 gcatttggat ataggtttta tcgatattct tacccatttc tccgcctaac gcatcgattt
    12661 ctcgaacgac aacgccttta gctggtccgc ctacagaagg attacatggc atgaatgcta
    12721 ccatgtccaa atttattgtc agtaataatg tttttgatcc catacgtgca gcagcaagtg
    12781 cagcttctga accggcgtgt cctgcgccaa caacgatcac gtcatacgca tctgcttcat
    12841 aatgttccaa tcgtttgttc ctccttttat tttcctaaac aaaattgact gaataattga
    12901 gtgattagct catcttgtac actgtcgccg acaacttctc ctagatagtc ccagcaacgc
    12961 gtcatatcta tttgaacgag atccacaggc attcctgaat caatacctgc aatgacttct
    13021 tcaagtgaga gggaagcttt ttctaacaac gcgatatggc gggtattaga aacataagta
    13081 gcgtctcttt ctcctgtttc tccgctgaag aataaatcag aaatagctga ttccaaacga
    13141 tctagtccgt cgtttttagc tacggaaatt gaaaaaactg gttcattttc gatcaatttc
    13201 ttcaattttt cttgttccag ctgtgctggc aaatctgttt tatttagaag aatgatccgt
    13261 ttcaatccac ttgtcgcttc caataattgt tcatcttctg ctgtcagtgg ttcgctttgg
    13321 tttagaacaa gtaagatcaa gtctgcttct gctaatgctt ttcggcttct ttcgaccccg
    13381 atccgttcca ccacatcttc tgtttcccga attccagctg tatcgattag cttgagcggt
    13441 accccccgaa cattgacata ctcttcgatt acatctcgag tcgttcctgc aatatccgta
    13501 acgattgctt tttcttctct taacaaatgg tttaacaaac ttgatttgcc gacatttggc
    13561 cggccgatga ttgccgtact taacccttcc cgcagaacct tcccttgctt ggatgtagca
    13621 agtagcgctt ggattctttg ttggatcatc tgcgcttttt caagcaataa cttcgtagtc
    13681 aattcttcga catcatcata ttctggataa tcaatattta cttctacttg tgctaatgtc
    13741 tctaaaatct cttgtctcaa tgagcggatc aaagcagaca gattgccgtc taactgatta
    13801 agagctagtc ccattgcttt gtctgttttt gcccgaatca aatccatcac agcttctgcc
    13861 tgtgaaaggt caacacgacc attcaagaat gctcgttttg tgaattcgcc tggctcagct
    13921 aatcttgcac cttctcttaa caatagctgc aaaatttgat tgactacaac gatcccaccg
    13981 tgacaattga tttctactac atcttcccgc gtaaatgtct ttggtgctct catcacggag
    14041 accatgactt catcgacaag ctgattgctc ttgggatcaa cgatatgtcc ataatggatc
    14101 gtatgacttg gtacttctga taatcgtttg tttccagatt gataaacttt atctgctagt
    14161 tgcacagctt gatcgccact taaacgaacg atactgattg caccttctcc aggtggggta
    14221 gaaattgccg caatcgtatc aaattctaaa gtaatttcag ccattttttc acttcctttt
    14281 tcattttgcg cacaaaaaaa gtgcccactc caccctttat gcacaataaa cggtgaactt
    14341 cgcactttga ctgcttgttc ttgattaaat tctggtcata gattatcaca cttgctataa
    14401 ggaatcaagt gtttaaaaca attagatgaa gttgagcctg ttctgcttcc attaccttat
    14461 atttaattgt ccttggatgt ctctttgcgt tctttggttt attcgaagag aagatactgt
    14521 ataaacaagg ctttatcttt cttaaaaaaa acggtacact aaatggtagc gctatctaaa
    14581 ctatacactt ttgaaaggag aaaatatgtg agcccgttca gatctgctgc attggaagaa
    14641 gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag ggaatatcaa ccagaaatat
    14701 atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag ctgatttgtt tgacccatct
    14761 tttcccaaag atgacctttt cttaacaacg aagaggaaat taaagacgaa ggtacttgaa
    14821 tatggtgcga atgcagtaat caactgtcat tttgagtatc agcaaaaaaa acgcgacgga
    14881 aagtattttc tggaactttt tgcttatgga acagtgatcc aatttatcca aacaacgatt
    14941 ggttaaatga aacagacaaa aaaagcggat caaaactgaa gacagtttcg atccgctttt
    15001 ttctatcaaa gttagtgact acttcatcgt atactctgat aagaatgatt tctagtaaaa
    15061 tatacttggt ttgaaaaact ttgtcaacgt gatttattca aaaagtaatg gaatcaataa
    15121 tattttttag caggttctac tactaaataa cgataaggtt cctcaccttc cgaatgtgtt
    15181 tttacatgtt catctttgct taatgcagca tggatttgtt tacgttcaaa tgcaggcatt
    15241 ggttctaaga aaacaggctg ccctgtccgt tttacttttt cagccgtgcg atcagctaaa
    15301 cgctgaatga tctcttggcg cttttcacgg taattgccta cattgactac tacagacagc
    15361 ttatttcttg ctaaacgatg gatgaagact tgtgccagat attgcaatgc attcagtgtt
    15421 tttccatgtt tcccaatcag tatgccttgc ttttgtgttt ctaaatggaa aacaaccata
    15481 ttatttgatt ctctttccat tttgacaaga gctggagcat caagctgttt tgaaatttca
    15541 gtaagataaa ctgccaattc cgttaaagct gcttcatcac ctagctcact tacttcttct
    15601 gttttttctt caagaatcgt ttcgttgact acctcgatct cagctgttgg ttcttcttcc
    15661 aaaatttctt ctacagtctg ttctactgtt tcagaaactt tttcgctgat acttggctgg
    15721 atcgaaagcc gtgcattctt tcgacccatt cctaggaaac ctttttttcc ttcttccagt
    15781 atttcgattt cgacttggtc tttggccaaa cccaaatcag ccagacctag ttggacagct
    15841 tcgtctattg ttgcagcttc ataaatcggc attagcatta cctcccctat tatttccgtt
    15901 ttttcttagg attcattgct ttcttaagag cacgttctct ttcgcggatt ctccgtgctt
    15961 cttcttcacg ctcttttcga attttgaaag ggttgttgat cagcagcgtt tgtacaactt
    16021 ggaaggcatt ggaaactacc cagtataatg aaagaccgct ggctaaattg acacccatca
    16081 ctaaaatcat tacaggcatc acgaaattca tgatcttcat tgatgcattc gattcaattt
    16141 gactcatgct agataaatac gtacttgcaa aagtaaagat tgctgctaaa atcggcaaaa
    16201 taaatgttgg atccgcgttc cctaagttca accataagaa atgcccttgt ttcaactctg
    16261 gtacacgtga aatagattgc cataacgcca tcaagattgg catttgtact aataaaggaa
    16321 gacaaccagc atatggattg acgccatttt ctgcatataa acgctgctgc tcttctctta
    16381 acttactttg tgtttccgga tctcttgaac tatattgttg ctgcagtgct tttaatttcg
    16441 gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa atgcatcaac ggcaatagga
    16501 tcacgcggat gatcagtgta aataaaatga tccctatccc tgcattacca aatgacaacg
    16561 ccttgatcgc ctcagcaaag taataaacaa tataacgatc ccaaattcct gtactctgtg
    16621 cgctgacttc cccagttccg caaccggata agacgatgac caaactaacg ataccggcca
    16681 tcaaaagtaa acgtttgtat ttcttcactt actcttttcc tctctgtcga ttatttttgc
    16741 aagttttaag acgtggacta aattggagcg aacctcatca taagtcaacc ccttcacact
    16801 tggtctagca atcacaataa aatccaactc cggatcaatt tcttctttta tagaataaac
    16861 tgctgctcgg atttttcgtt tgacttcatt gcggtttacc gcgttgccga ccttttttcc
    16921 tacagataaa ccaactcgaa aatgaggctg tcccggtttt tccaaacggt aaacgacgaa
    16981 tttgcggtta gcaaaagaat ctcccgtatg gaacacggct tgaaactctc gttctttttt
    17041 tactcgatac gcttttctca ttagttttct ttccttttct atctctcact tacgctcata
    17101 atcataccat aattacgatt gtcctttcat catttcccta aaaaagagta taaaaaaaac
    17161 cactgaaacc ccagtggcct aagctgaaag aacttttctt cctttgcgac gacggctagc
    17221 taacacacga cgtccatttt tagtactcat acgtttacgg aatccgtgaa ctttttgacg
    17281 tttacgttta tttggttggt atgttctttt catatattcc acctccaaaa tgttct
//
LOCUS       DAZKNM010000047        17263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000047 DAZKNM010000000
VERSION     DAZKNM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.047"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..572)
                     /locus_tag="ACG8M9_001255"
     CDS             complement(27..572)
                     /locus_tag="ACG8M9_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0378041.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(584..1276)
                     /locus_tag="ACG8M9_001256"
     CDS             complement(584..1276)
                     /locus_tag="ACG8M9_001256"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0378042.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1295..1579)
                     /gene="macP"
                     /locus_tag="ACG8M9_001257"
     CDS             complement(1295..1579)
                     /gene="macP"
                     /locus_tag="ACG8M9_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0378043.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1592..2155)
                     /locus_tag="ACG8M9_001258"
     CDS             complement(1592..2155)
                     /locus_tag="ACG8M9_001258"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0378044.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2383..3045
                     /locus_tag="ACG8M9_001259"
     CDS             2383..3045
                     /locus_tag="ACG8M9_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0378045.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3169..4365
                     /locus_tag="ACG8M9_001260"
     CDS             3169..4365
                     /locus_tag="ACG8M9_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0378046.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4466..5842)
                     /locus_tag="ACG8M9_001261"
     CDS             complement(4466..5842)
                     /locus_tag="ACG8M9_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0378047.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5879..6745)
                     /locus_tag="ACG8M9_001262"
     CDS             complement(5879..6745)
                     /locus_tag="ACG8M9_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0378048.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6834..7859)
                     /locus_tag="ACG8M9_001263"
     CDS             complement(6834..7859)
                     /locus_tag="ACG8M9_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0378049.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7877..8551)
                     /locus_tag="ACG8M9_001264"
     CDS             complement(7877..8551)
                     /locus_tag="ACG8M9_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0378050.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8735..9628)
                     /locus_tag="ACG8M9_001265"
     CDS             complement(8735..9628)
                     /locus_tag="ACG8M9_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0378051.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9702..11399)
                     /locus_tag="ACG8M9_001266"
     CDS             complement(9702..11399)
                     /locus_tag="ACG8M9_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0378052.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11392..12705)
                     /locus_tag="ACG8M9_001267"
     CDS             complement(11392..12705)
                     /locus_tag="ACG8M9_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0378053.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12705..16646)
                     /locus_tag="ACG8M9_001268"
     CDS             complement(12705..16646)
                     /locus_tag="ACG8M9_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0378054.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16755..17213)
                     /locus_tag="ACG8M9_001269"
     CDS             complement(16755..17213)
                     /locus_tag="ACG8M9_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0378055.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4832 a         3887 c         3009 g         5535 t
ORIGIN      
        1 ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt ggactcaaaa
       61 tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggaatc tttgaaaata
      121 tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca gatatctgta
      181 catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat gtctagatct
      241 gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc ctcttgctca
      301 tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt tcctatcaca
      361 ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc atgcgcatca
      421 atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc agtcagcttt
      481 ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc aacaaaatca
      541 tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg agatgtttga
      601 caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat tcgtcaaagc
      661 tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa aatgggatat
      721 tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct aatgcttcag
      781 ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta tcacctgtaa
      841 cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc atttcactgc
      901 gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct gggttgtagc
      961 caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg atgtcaccta
     1021 cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg ttcacgccgt
     1081 attgttggat caacagactt gtggtaatag atgctaagac ttttcctata ccggaacgta
     1141 cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct gcgcgttccc
     1201 atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc attgcgccga
     1261 taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt gctaaaaaaa
     1321 ccacacataa cattaggata acaataataa gagatttcat caaaaaagag ttccatttgg
     1381 tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt ttgctttctt
     1441 tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct gcttttcgct
     1501 gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg cttcgcgtaa
     1561 taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat agttcccagt
     1621 attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc gcttgttgcg
     1681 cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt gctaaaggat
     1741 tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt gcaaaacctg
     1801 gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca gtttcttctt
     1861 ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg atttttccag
     1921 ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca agaagcagac
     1981 gatcttgttc gtcaaaagca atgatcccga caccgccagg atgaaatacc agttctctct
     2041 tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata atttttccgc
     2101 tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc agcatatctt
     2161 ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt tctcaaaaac
     2221 tccttattag tttaccatag ataaacctta gattttctct aaatatagaa aatccttctt
     2281 tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc gaataacgaa
     2341 aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa acgaaattgg
     2401 ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag tttttctttt
     2461 gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt tggttcgcgc
     2521 aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga aactcactat
     2581 gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat gaatcaaaca
     2641 aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact taaagcaatc
     2701 gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt ggtgaaagaa
     2761 ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa tctagttgat
     2821 ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca gacctatgaa
     2881 aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc acaagattat
     2941 caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt tgcgaaacag
     3001 agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt ttgttcataa
     3061 aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt ctaaagtaga
     3121 gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat ggatcataaa
     3181 ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt attgaacaac
     3241 ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat tgatcagctc
     3301 caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg ccaagaacaa
     3361 gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat cagctacgga
     3421 tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca tgtgcaagca
     3481 caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct ccaagaagcc
     3541 aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt cgggaaggtc
     3601 aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca aattgacaag
     3661 atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct gatgttagaa
     3721 cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat cgctgctgga
     3781 gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa aagagcagaa
     3841 gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt cctagatcgt
     3901 ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca gcaagcacca
     3961 caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca agcatctatc
     4021 gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt gcttcgtcaa
     4081 aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt attgaagaaa
     4141 aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga acgtggaatc
     4201 gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat ccaagagaca
     4261 ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga gcttggacac
     4321 atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac ctttcgcttg
     4381 ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt ttttcttatc
     4441 tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt ccatagccat
     4501 agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc catgtcacag
     4561 tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc aatagcggaa
     4621 cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc aacttcagta
     4681 agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt tccgctaata
     4741 cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca taagtcgctt
     4801 ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt aagccatcct
     4861 tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt accgtcagta
     4921 gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct acatacctca
     4981 ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt cctgtcaaga
     5041 tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg attcctcttg
     5101 gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca tcaaatccca
     5161 agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca aaaggcatga
     5221 atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac aacgcgatga
     5281 taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg gcttcaagcg
     5341 acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg tatgtaccat
     5401 tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa cttttcaaaa
     5461 aaccttgcat atagccagac catccggaag caacagagga aaccgctagt aaaaattccc
     5521 cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca tacagatagg
     5581 catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt agtccagaca
     5641 aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca gcattttgat
     5701 tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca attccaagca
     5761 tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg agattgatct
     5821 ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa aacagcgatt
     5881 aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg caaccaacaa
     5941 taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc ccacctaaag
     6001 tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca agtgatttga
     6061 ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc atgtttttcc
     6121 acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct aagatcattc
     6181 cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg acgatgatgg
     6241 tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta ccaaaatcaa
     6301 gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc aaatacgcac
     6361 cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt tgtgtccatt
     6421 catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca gtagacaatg
     6481 gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt tgtccaacac
     6541 tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt acaggactaa
     6601 caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct agtgtttgtt
     6661 gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat gccaacatag
     6721 ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg ataagaaact
     6781 tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat tttctaacgt
     6841 ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc ttttccttca
     6901 gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc taaataaagt
     6961 cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa tcccttatca
     7021 aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac tgaaaggctg
     7081 actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg cgtatatttg
     7141 atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc ggtcaaaaca
     7201 gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata atttgcctga
     7261 ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag gtagtcccgc
     7321 gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc gatttttgat
     7381 aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat cgaacgaact
     7441 agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc aatataatct
     7501 ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc acggatcagc
     7561 tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact caaataattt
     7621 tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct cttgctcaag
     7681 taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa ataagcaatt
     7741 gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt caatgcaata
     7801 aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa aaaattcatc
     7861 ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt tcgatatatc
     7921 cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact gtcaatgtat
     7981 tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc cgacttaaga
     8041 tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg ctcaaatcaa
     8101 tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg ccattgtggc
     8161 tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat gcattgattt
     8221 ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc cccatgttca
     8281 tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc ggtactttcg
     8341 atacttcacg tattttttga caccaataat agccatccaa aacgggaaga ttaatatcta
     8401 gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg aaatcggtcg
     8461 ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt cgaatgatct
     8521 cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt cttcttgcta
     8581 ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat gtcattttag
     8641 caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt ccaagcagct
     8701 taaatttatt atgttattct taataattgg tctattatct gttatttttc tttcgttgtt
     8761 tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact agcaatatac
     8821 ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct atctttgaag
     8881 agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga gtgttcacgg
     8941 tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt gctaatgtat
     9001 ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc atccgaccat
     9061 acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta aagagttttt
     9121 tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct ccagttggaa
     9181 aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc gtttcatcaa
     9241 ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata tgttgttcgt
     9301 aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga tctttgactt
     9361 gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt tcatgcactg
     9421 ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga taattctcga
     9481 tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat gtaaaaactg
     9541 cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg atttgggttt
     9601 gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa aaaactgcag
     9661 caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc agacttcatt
     9721 ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat tagtaagaat
     9781 gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt cggtactctt
     9841 tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct tccataagag
     9901 atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc tggcgccatg
     9961 gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta tccatttcca
    10021 tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc tgctcctgat
    10081 tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga acggtcttca
    10141 ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc atatttttca
    10201 aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc ataatcaaat
    10261 tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc cgcttctagt
    10321 tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc atctgctaat
    10381 ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt cacgacaaac
    10441 ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt ctcgccggct
    10501 cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc tgtgattttg
    10561 taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata aggaataggt
    10621 gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga aatcgtttct
    10681 tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc tacattcatc
    10741 tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt attgtatgtg
    10801 tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat cactggcatc
    10861 ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt ttctaagaaa
    10921 agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt agagaatacc
    10981 gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata ttttaaaaac
    11041 caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt aagaccgtct
    11101 tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc cgttacgtca
    11161 aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga aaccaatccg
    11221 ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt aatcgtcact
    11281 tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc gaaaatccct
    11341 attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt tttattcatt
    11401 tttttctccc cgttcttttt ttattaataa aacagcacct aacaataaaa gaattcctag
    11461 taaagaaatc aacgttcttg cttcattcgt ttgcgggaat acggaccatt ttcctgattg
    11521 acctggattg cttggaccac tcggctcttt cggtttgtcc gttgtttctt ttacggtctc
    11581 gttataaaca cgtggtgttg cggattcata ggcagagact ggaactttcc cggattctaa
    11641 ttcagccatc tcctgtccgt taactactac atattgtggt tggccttttg catccaccca
    11701 aaattcaggg atgatgacct cgattctctt acttgcttca tccatctcgt aaccttccac
    11761 accttttaat tcttcaaaat aataggtgcc agaaggcaag aaacgttgtc ctgtcgtaac
    11821 aaggccctct ttatccgaaa tgaatttgct tacatttttg tcattcaaag gatcattgtc
    11881 tgctggtttg atccattgat ttttcaagtc attagcagga gccatatcta aataatattt
    11941 ccccccttct atcatctgat atagagcaaa aacagcacct tctaatggtt ttcctttttc
    12001 agttgttcct ctttgacgtc catacttgaa gaagtatgga tctcttagat aaccgacatt
    12061 tttaggatag atatgaatct cttgcagttc ctcggttgat tctgtcgggt gataaattgg
    12121 caaaatagca gcaattggga tagctgtctg atcgatatca acatccaatc caacttctgg
    12181 atcaatcgat tcttcgaaaa tcaaataaac tgcatcccga tcttggtttt tcttaggtgc
    12241 tttgatacgt gcaatcccgt ctcctaatcc gccaaccgta tctttcccag ttgttaccga
    12301 agtccctatt tggacaagcc tttcttctgt gattagctga cggatattgt ttctgttcgt
    12361 ttcagcgatt tgctggacga attctttagg agtcatgttt tcagcagctt ttttttcatg
    12421 ataataatcg gtcatgtcgt aaatggcaaa tgtcgctcca tttagtggca ccgtctgtga
    12481 aatgatatct gctgtttccc ctgcttcctc agagatagcc aagccatcat tttcatggta
    12541 ttcaaattga tcgttataat cgatatctcg aatcatccgt ttatgcaaca cgaattccac
    12601 gacatcttcc cctgattctt ctgccgacat cgcatgtgga agaaggactg ctgcaagcgg
    12661 tagtaaaaca agtaattgaa gcatcaaaca caacagcttt ttcattacct tgctccctcc
    12721 tttttagatt gtctgacaaa caaaccatag gctgctcctg caagcgccat gaagaatgca
    12781 gcaagcagga tatagaataa tcgtccggat cctcctgtag caggcaacgg attttcagga
    12841 tcgttcccga cactaagctg taatctgatt cgatttgtct ccaaaagttc atattctaac
    12901 tcttgctgta gatcaggcgt ctctgcttct tcgtttggat aatacgaaat ctcaactttt
    12961 agtgtacccg cttcttttcc tggaactacc cgatcatttt ctatgtcagg ttcttctcga
    13021 taactaattt ctaaaataaa gtaacctgga agttttctat agctgtcagg tgacttcact
    13081 tccttgatcc gatagacccc ttccaacaag tctgaagcat tgaataggga agaatcgttt
    13141 ggatctggtg ccaagcctcc ctcgatgatt tcatactctc ctgcactatt ttgtttctca
    13201 attgaaaatt ctgcgccttc aagacgtcgg ctattcatac tgtcaatttt caaaagttcc
    13261 aaatcgattg gcgctagttc atcttctaag gttacttgta gtacaccatc gatgatttca
    13321 tagccttccg gcaagggagc agtttcgtct tcgatgaccg tatcttcaat gataaactta
    13381 ccttcttcat tcagtgcaaa ctggaacact tgatcagata gccgataccc atttggtgcg
    13441 acagattccg agacctcata ttcaccaata gacagatgca gaggttggtc gtaatttaaa
    13501 tcgacatact tttctactcc tacggcacga gaatagaagt atccgatccc aggcaaacgt
    13561 ttcgattctt ctggttctaa cggattacca gtgtatgctc gatacagcgg gaaggaagaa
    13621 tcccccactt tcctgatatc gaaacctgca ccatctaatg tatggccttc atggtctaat
    13681 tttctaactc ggatctcccg ataatcaaac gtattgtaag cagtgaatgc tacccataaa
    13741 cgatcctctt catctgttgt atacgtgaga tattgccaat tttcttcacc tgtttgcttg
    13801 agttggatag tccctatagc attatcgtaa agtcgcaaga tccatgtgtc attttcatca
    13861 ggttctttga aaccttctgg gttctggctc tcaatgattt tgtattctgc atagttggta
    13921 gtttcttcac tatccttata aacaaatgaa tctggctctc cgattggatg aataactttc
    13981 tcaccatcca cgattacata tttcccgtct tcatctactg aatagaacgt gagtctccct
    14041 ttctcttcct ctacttcgtt cacaccagaa agccctttcg ctactggata tccgatttcc
    14101 agtccgttac catctgtttc ataaagttca aactctgcgc cattgattgg catattactg
    14161 ccttcaatta gtttattgat attcatgtgg attggcttga attcatttcg catttccagt
    14221 ttgacataca actcatcata ctctgtgcct tctatgttag gctcttgtac aacagaaagc
    14281 ttgtgatatt ctccttcata ataggaattc ggatctttac cttccggtcc gccgactctc
    14341 gaaccagatg tatattcatc tacaagctcg tagtgaccta acatctcatc ttcgttggct
    14401 gcatattttt tgatttggaa ttgatacgtc tcatctacta actgataacg tggtggtcca
    14461 gcgatctctc tgatccaaaa tatgtcgctg actgctccag tttcatctcc taaaatcggc
    14521 atcttcgttt caaagatcgc tattccgtcc gtggtagttt tacttgtttc gattggttcg
    14581 gcttccccac tctctggatt ttggtcatat aatgcaaatt cgactccagc caacggctcg
    14641 ccattttgac ccttcttgct gacttggaaa ttacggcctt tatgttcgta acgaatggaa
    14701 gaagatggga aatacatccg tccagtagtg atatcattct cataatcctc caggttctga
    14761 atataggcca tattgttcac tgtgcaaaaa tttccgtctt gagctcccgc atcaagtttt
    14821 acttggtact tgatcctgac catttcccct tcacctaaag aaatcggaga aaatttataa
    14881 gcttggtttt cccgtccgac atacgttgtc ggagtcgcca ctacttctgg tggggcttgt
    14941 ccatgacgtt cttcatccca tatttctggc tcttggtact tcatgccgtt ttctcctgga
    15001 tctagatggt aaaactgaat atctgccgat ctgccagctc caaatggtga gacattaaaa
    15061 ccagtttcat cataaagtgt ggcttgctgg atcgttctct tgaaggtaga agagatatcc
    15121 tccaaggctg taacaatgtc attcttgttg ttcgcattgt aatatcgcgg tgttacgcca
    15181 tcttcttcta aatcagcaat atttttcaat acatttcgtc caatagcatc tagtcgttgt
    15241 ctcccgctaa cgtttccatc gattcctaaa ccaatggaga agatacttgg cgggttctct
    15301 tgatacttgt ttttcagcca atgatgataa gcaactgtgt tgatcgcaga tgacttttct
    15361 tgcgaagtag gactgccatt cgtattgtta ttagaagaac catcaccaac ccagtgagtc
    15421 gctttattat ctccttcttt tacctctcca taacgatagt gtacatttac ttcatcttct
    15481 ggacggttaa agtcagatgc ataggtaact ggatgagtaa accctgcatt agaagtattc
    15541 ccagaaaact cctcaaaatt tctgaacaaa acgtcattat cttcagcaat cttatcttcc
    15601 atcacagtcc agttgctcca agctccatta gtgctcgtac gataacttga ctgaatcggt
    15661 gcataactga aggtcggtgt cccatctccc acaacgatta ggattttttc tggatttcta
    15721 ttttctccgc cattgtcttt atacaatgtt tcatagccat ttttaagccc tagtgtaagc
    15781 ggagttcctc cgattggtgt acgatttaat gctgtattct cacttcccgt caaacgattg
    15841 atatcatttg tcaatggaaa gatgtccgaa ctgatttgct catggttatt gatagctgta
    15901 ctattatggt aaaaattcac cataccgatt cgaatatcca gactcgggtc agagagcaga
    15961 tctttactca tttcgtttac tgcctcaatc aacgcagcat ttttagtttg actattggtc
    16021 ccagcggtta attcactcat actcgctgac ttatctagaa caaaaacgat atcgattgga
    16081 ctgatttcct ttttctcgcc cccgatgaca tctagataga cattgtaaag ggttgttgca
    16141 tcttcaggat gtagatcaga tgctgtgctg cttggcagaa caggttctac ccattttttg
    16201 atgtacgccc cgttataatc caagtaactg ttttcaaaat tccgttcatt tccccaaagc
    16261 tgaacggttt gagctggaac gtttctatct tcttcgcctg cagttgatcc attattagta
    16321 gcatagtcgt aatttcgcca gttcgctccc ccgtttgtat aatcactatc atcgatcaag
    16381 ttctctccgt catatggcga gcctttgtct ccttgatagc gttcattgct ttgtgtccaa
    16441 tttcctaaca cttttgtata gtcattcgtt gtaaacttcg ggtagcgatg ctgttcgttt
    16501 tcaacttctc cgctaggata gatatattca taggggtctt gcgtagctgt tggcccaaga
    16561 cgctccaaaa taccgttgaa taactggcgt tgaatgcgct ggagtgcttg accgctgggt
    16621 acgattggtt cgtccagctt ttccatctca ttttcttcgc tacgttgaat ttcagcggaa
    16681 tcttctgtat cagtttcatt ttcttgtaat tcttggttat ctattctttc atcaaccacc
    16741 acttgaagag gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat
    16801 gtgccggttt ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc
    16861 gtctcgactt ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg
    16921 ttttttacgg taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca
    16981 ttggcacttt cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata
    17041 tacttccctt caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct
    17101 gttgactcag cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca
    17161 aatagcaaac tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta
    17221 tactcctttc aaaaatattt gtcttctttc acaacaatca aca
//
LOCUS       DAZKNM010000048        17169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000048 DAZKNM010000000
VERSION     DAZKNM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.048"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1881
                     /locus_tag="ACG8M9_001270"
     CDS             115..1881
                     /locus_tag="ACG8M9_001270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0378056.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            1983..3242
                     /locus_tag="ACG8M9_001271"
     CDS             1983..3242
                     /locus_tag="ACG8M9_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0378057.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3389..4690
                     /locus_tag="ACG8M9_001272"
     CDS             3389..4690
                     /locus_tag="ACG8M9_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0378058.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4693..5544
                     /locus_tag="ACG8M9_001273"
     CDS             4693..5544
                     /locus_tag="ACG8M9_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0378059.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5557..6372
                     /locus_tag="ACG8M9_001274"
     CDS             5557..6372
                     /locus_tag="ACG8M9_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0378060.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6523..7689
                     /locus_tag="ACG8M9_001275"
     CDS             6523..7689
                     /locus_tag="ACG8M9_001275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0378061.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7919..8566
                     /locus_tag="ACG8M9_001276"
     CDS             7919..8566
                     /locus_tag="ACG8M9_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0378062.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8650..9594
                     /locus_tag="ACG8M9_001277"
     CDS             8650..9594
                     /locus_tag="ACG8M9_001277"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0378063.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9729..11180
                     /locus_tag="ACG8M9_001278"
     CDS             9729..11180
                     /locus_tag="ACG8M9_001278"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0378064.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11201..12037
                     /locus_tag="ACG8M9_001279"
     CDS             11201..12037
                     /locus_tag="ACG8M9_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0378065.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12037..12696
                     /locus_tag="ACG8M9_001280"
     CDS             12037..12696
                     /locus_tag="ACG8M9_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0378066.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12822..13511
                     /locus_tag="ACG8M9_001281"
     CDS             12822..13511
                     /locus_tag="ACG8M9_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378067.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13517..14473
                     /locus_tag="ACG8M9_001282"
     CDS             13517..14473
                     /locus_tag="ACG8M9_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0378068.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14517..15029
                     /locus_tag="ACG8M9_001283"
     CDS             14517..15029
                     /locus_tag="ACG8M9_001283"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378069.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15285..15833)
                     /locus_tag="ACG8M9_001284"
     CDS             complement(15285..15833)
                     /locus_tag="ACG8M9_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0378070.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15922..17103)
                     /locus_tag="ACG8M9_001285"
     CDS             complement(15922..17103)
                     /locus_tag="ACG8M9_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0378071.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5632 a         3067 c         3448 g         5022 t
ORIGIN      
        1 aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc gttgtctttt
       61 gtttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa atcaatggat
      121 acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac gaatacaaga
      181 tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt gctttgtgga
      241 gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt
      301 ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac aaggcgctta
      361 cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg tgatcaagga
      421 atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg aataccttat
      481 tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt atggtatcaa
      541 atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca cactttacct
      601 tggggaagta aagatccaga ccgagaagac ttcttcgggg gagatttgca aggtgtgagg
      661 gatcatttgg attacttgac ggaccttggc gtcaatggca tctatttttg tcccatattt
      721 aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga tcccgctttt
      781 ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg gatacgtatc
      841 atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca agacgttctc
      901 aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt cccagtagat
      961 tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga tacttttgct
     1021 tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa atatttgctg
     1081 gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct tgatgttgct
     1141 aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga aatcaaacca
     1201 gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca aggggatgaa
     1261 ttccatgcag tgatgaatta tgcattcaca gattcgatta aagattattt tgcaaaaaag
     1321 aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct ttacagagat
     1381 caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag gatcttgacc
     1441 ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa
     1501 gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga tgacccggat
     1561 tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt cggttttata
     1621 aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg ttctactcag
     1681 tggctaatag tatatgatcg tgaagataag ctctatttca cacgggaact agagggacag
     1741 atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag
     1801 gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat acgacaatac
     1861 ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg
     1921 aagatttgtg cccattataa taagggtgta acagcacgtt gaatacacag gaggaaatga
     1981 agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta ggaatcttag
     2041 gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc gatgaaggaa
     2101 aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa ataaaaaaca
     2161 ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg ttcgatcagt
     2221 tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg atggcagcat
     2281 atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg
     2341 aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa atctacggag
     2401 aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc gatacagcac
     2461 catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct tttgagtcag
     2521 aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat tcctatggtt
     2581 taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct tcagatatcg
     2641 gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg
     2701 tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc cagcaattca
     2761 tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca tacaaagaaa
     2821 acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag ccttatcaac
     2881 cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa gacctctctc
     2941 aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa
     3001 atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac gatgaattaa
     3061 caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg
     3121 gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac
     3181 caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa caaaaatact
     3241 gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca gataaaagtg
     3301 gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg
     3361 gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca atatcaacga
     3421 aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa atggccaagc
     3481 ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga tgcttctggg
     3541 cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg aagatcattc
     3601 attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt tcttgatttt
     3661 ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa
     3721 aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt ttccttatct
     3781 attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag tcctagttac
     3841 attgttcact gcatttacta attatgactt caatcatatt ccaccagcca gtttaattga
     3901 ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc gtaatacttt
     3961 ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt tgcaaatcac
     4021 ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca aacgtatttt
     4081 cggtatcatt ttcttattac catgggcggt cccagctttt attactatca tgagtttttc
     4141 gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt tcataaacca
     4201 gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt
     4261 agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga tggttacagg
     4321 aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg gagcaaatgc
     4381 atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt
     4441 tattacacag tatacgggga atttcaataa tttctccatg atctatctgt ttaacaatgg
     4501 tggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt cctggatcta
     4561 taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca cgttgattat
     4621 ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg cgttcaatat
     4681 gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg actttctcac
     4741 ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc gcttttgatc
     4801 acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga tctttcaagt
     4861 gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg gacttggtat
     4921 aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt agtaactttg
     4981 gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt gatgttcttt
     5041 ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt catggctttc
     5101 ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg cggtggaatt
     5161 ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga tttagatgaa
     5221 tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt attaccgctt
     5281 gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt tggggatttc
     5341 atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa
     5401 acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg
     5461 attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt taccggcctg
     5521 acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc gttaaactat
     5581 ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt atcatggcct
     5641 aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt gactcttttt
     5701 tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca ttcgctgatc
     5761 gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg gttacaagca
     5821 tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt atctgagaaa
     5881 caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt gatccaacaa
     5941 aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt ccaaccagat
     6001 tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta tgccagtaat
     6061 cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt tgtcatgacg
     6121 agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag ccaactttca
     6181 tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg aataggttcc
     6241 atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct aagtgtccaa
     6301 acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt caaagataag
     6361 cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat
     6421 aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc ttttcttttt
     6481 tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga gaaaacttta
     6541 tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta ttgggacggt
     6601 acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa tatccatgaa
     6661 gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga agcctatatg
     6721 gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc ttatcagcaa
     6781 gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa agaaaaacat
     6841 acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa cgacttttat
     6901 aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac acctgaagat
     6961 acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact ctttatcaaa
     7021 gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt cactgctacc
     7081 aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata cgatttgatt
     7141 accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt aatggattac
     7201 cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt cgaacatgta
     7261 ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa accaaaagga
     7321 acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc ttggatcaat
     7381 aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga taatattaca
     7441 aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca attgacatta
     7501 gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga aatgggtgat
     7561 tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt tgaatcaagt
     7621 aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat cccaatgcat
     7681 cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc taagttttat
     7741 gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa gcttagtttc
     7801 tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa
     7861 ttttttcttc gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat
     7921 gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc
     7981 aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca
     8041 ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag
     8101 tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca
     8161 agataaatac ccagatactg caattactag tttggaatta gatagtgatt ggggatctta
     8221 cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca
     8281 aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca
     8341 aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa
     8401 aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt
     8461 aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat
     8521 caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa
     8581 aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga
     8641 gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg
     8701 aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa
     8761 gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca
     8821 cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt
     8881 tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg
     8941 ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata
     9001 gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga
     9061 aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc
     9121 atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat
     9181 cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg
     9241 ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg
     9301 ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt
     9361 tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa
     9421 gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac agagcttcat
     9481 aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa
     9541 aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga
     9601 gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct
     9661 gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg
     9721 aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt
     9781 ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg
     9841 cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag
     9901 aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg
     9961 gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta
    10021 attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag
    10081 agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga
    10141 accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa
    10201 tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt
    10261 gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga
    10321 gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg
    10381 ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg
    10441 agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga
    10501 tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg
    10561 gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca
    10621 cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag
    10681 gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat
    10741 atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag
    10801 caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat
    10861 ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac
    10921 acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga
    10981 gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct
    11041 tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag
    11101 gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt
    11161 taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt
    11221 tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca
    11281 ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg
    11341 cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga
    11401 tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt
    11461 ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg
    11521 tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga
    11581 cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg
    11641 gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc
    11701 taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg
    11761 tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc
    11821 agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt
    11881 attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa
    11941 agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa
    12001 accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcccct aattgatttt
    12061 tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt
    12121 ggcttcgtgt cgtttctttt aggaaatttg gtagggttgc ttttgacagt tttaggtctt
    12181 ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt
    12241 cccgcactag tcctgctgtt tctgttatat ttcgggttac cttatcaatt aagtgcctta
    12301 acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga
    12361 ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct
    12421 ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca
    12481 ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg
    12541 ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc
    12601 tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag
    12661 tatttagaaa aacgttctat ccatcagtca atctgaaaaa tagaaatggt gctagcgtgg
    12721 ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc
    12781 atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca
    12841 tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag
    12901 aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg
    12961 atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta
    13021 cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg
    13081 aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg
    13141 ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag
    13201 agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag
    13261 taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaaaagat caattgtaca
    13321 aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac
    13381 ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc
    13441 ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc
    13501 cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc
    13561 attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc
    13621 agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcc
    13681 aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca
    13741 cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa
    13801 tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc
    13861 aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga
    13921 tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca
    13981 aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta
    14041 tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg
    14101 ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc
    14161 ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca
    14221 agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca
    14281 tcaactcttg attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt
    14341 ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca
    14401 cctgctagat catccaaaag attggcttaa atattattca aaagttcagg aactatatga
    14461 agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt
    14521 atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag
    14581 cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag
    14641 aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa
    14701 ttgctggaac agctgcatta gtctcaggcg tggatcccgt ttacacaaaa atagatggta
    14761 gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta
    14821 ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca
    14881 aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca
    14941 tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa
    15001 gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca
    15061 ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag
    15121 cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg
    15181 tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt
    15241 tgtcacgatc tctcggctta tcctacgagt attattcgat acatttatat caataatcct
    15301 ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag
    15361 gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct
    15421 gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag
    15481 atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca
    15541 tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta
    15601 ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc
    15661 atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt
    15721 cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg
    15781 agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt
    15841 tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa
    15901 gtaactttgc ttttttgtat tttataccag caagccagtc acaatagcac ttaaaaagct
    15961 gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc
    16021 ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga
    16081 aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc
    16141 taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc
    16201 atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc
    16261 taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat
    16321 caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag
    16381 aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc
    16441 atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa
    16501 taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc
    16561 gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc
    16621 agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc
    16681 agaagctact ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag
    16741 accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc
    16801 agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc
    16861 tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat
    16921 ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag
    16981 aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact
    17041 gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact
    17101 caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact
    17161 aatctttag
//
LOCUS       DAZKNM010000049        16951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000049 DAZKNM010000000
VERSION     DAZKNM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.049"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8M9_001286"
     CDS             <1..1152
                     /locus_tag="ACG8M9_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0378072.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8M9_001287"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8M9_001287"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0378073.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8M9_001288"
     CDS             2728..3003
                     /locus_tag="ACG8M9_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0378074.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8M9_001289"
     CDS             3009..3776
                     /locus_tag="ACG8M9_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0378075.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8M9_001290"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8M9_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0378076.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8M9_001291"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8M9_001291"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0378077.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8M9_001292"
     CDS             7920..8222
                     /locus_tag="ACG8M9_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0378078.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8M9_001293"
     CDS             8225..9388
                     /locus_tag="ACG8M9_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0378079.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8M9_001294"
     CDS             9414..12842
                     /locus_tag="ACG8M9_001294"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0378080.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8M9_001295"
     CDS             12863..13987
                     /locus_tag="ACG8M9_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0378081.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8M9_001296"
     CDS             13968..14414
                     /locus_tag="ACG8M9_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0378082.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8M9_001297"
     CDS             14398..14736
                     /locus_tag="ACG8M9_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0378083.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8M9_001298"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8M9_001298"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0378084.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8M9_001299"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8M9_001299"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0378085.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8M9_001300"
     CDS             15893..16936
                     /locus_tag="ACG8M9_001300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0378086.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5745 a         2933 c         3739 g         4534 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa a
//
LOCUS       DAZKNM010000050        16423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000050 DAZKNM010000000
VERSION     DAZKNM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.050"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..494)
                     /locus_tag="ACG8M9_001301"
     CDS             complement(96..494)
                     /locus_tag="ACG8M9_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0378087.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(491..1264)
                     /locus_tag="ACG8M9_001302"
     CDS             complement(491..1264)
                     /locus_tag="ACG8M9_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0378088.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1261..1944)
                     /locus_tag="ACG8M9_001303"
     CDS             complement(1261..1944)
                     /locus_tag="ACG8M9_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0378089.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2111..2998)
                     /locus_tag="ACG8M9_001304"
     CDS             complement(2111..2998)
                     /locus_tag="ACG8M9_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0378090.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2995..3864)
                     /locus_tag="ACG8M9_001305"
     CDS             complement(2995..3864)
                     /locus_tag="ACG8M9_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0378091.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4001..5287)
                     /locus_tag="ACG8M9_001306"
     CDS             complement(4001..5287)
                     /locus_tag="ACG8M9_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0378092.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5560..6138
                     /locus_tag="ACG8M9_001307"
     CDS             5560..6138
                     /locus_tag="ACG8M9_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0378093.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6138..7067
                     /locus_tag="ACG8M9_001308"
     CDS             6138..7067
                     /locus_tag="ACG8M9_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378094.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7064..8800
                     /locus_tag="ACG8M9_001309"
     CDS             7064..8800
                     /locus_tag="ACG8M9_001309"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0378095.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8863..9699)
                     /gene="pheA"
                     /locus_tag="ACG8M9_001310"
     CDS             complement(8863..9699)
                     /gene="pheA"
                     /locus_tag="ACG8M9_001310"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0378096.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9696..10205)
                     /locus_tag="ACG8M9_001311"
     CDS             complement(9696..10205)
                     /locus_tag="ACG8M9_001311"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0378097.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10198..11493)
                     /gene="aroA"
                     /locus_tag="ACG8M9_001312"
     CDS             complement(10198..11493)
                     /gene="aroA"
                     /locus_tag="ACG8M9_001312"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0378098.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11506..12597)
                     /locus_tag="ACG8M9_001313"
     CDS             complement(11506..12597)
                     /locus_tag="ACG8M9_001313"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0378099.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12614..13780)
                     /gene="aroC"
                     /locus_tag="ACG8M9_001314"
     CDS             complement(12614..13780)
                     /gene="aroC"
                     /locus_tag="ACG8M9_001314"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0378100.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13782..14846)
                     /gene="aroB"
                     /locus_tag="ACG8M9_001315"
     CDS             complement(13782..14846)
                     /gene="aroB"
                     /locus_tag="ACG8M9_001315"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0378101.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14879..15901)
                     /gene="aroF"
                     /locus_tag="ACG8M9_001316"
     CDS             complement(14879..15901)
                     /gene="aroF"
                     /locus_tag="ACG8M9_001316"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0378102.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4740 a         3628 c         2913 g         5142 t
ORIGIN      
        1 tttcataagt attcacgaga aaaatacaaa caaaaaagcg gaaataacgc tcatccgcct
       61 tcttgtttgt gtttatttcc tcttcaaacg cttgtctatg attcagactt ttctctatcg
      121 ttattttcat ctaaaagttc ttgcattccg ataatttctc gatattgatg aatcttagtg
      181 ataccaatga ctttgtctac atcaatacta aaacaaatac cattacctgg aatatcgatg
      241 ttgatgcctt gaccaatcgc atccattact ttattcgtca aactatcggg tactaatgtc
      301 aacacaatat ctttttctgg cgaaatgtcc aaacctaaaa atttccctga atcatgtaca
      361 ccagaacctc ttccatgtaa gatcgttccg cctgctgcac ctgcttcttt tgaaaagtcg
      421 ataacatctt cgcccattcc tccgtcaacg atcgtcacaa tcatgtgaag attcaatgct
      481 acaagatctt tcatttgctt tcccccaatt tttgcaatct ttctttttcg attctgtctt
      541 tttcatcttt tcgtttataa atatatccta gtactaaagt ggataatatt ggtgtcaatg
      601 caatcattgc aaccacacca aacccgtcaa tgatcggatc tcttccttca atttgtgcag
      661 cgattgccac gctcattgat aaaataaatg tcgaacacat cgggcccgtt gctactccac
      721 cgttatcaaa agccattgct aaaaagatct tgtccacttt tcgtcctaaa ataaatgcta
      781 gcccgtatcc tgggatcaag aaataataaa gagagaatcc gacgagcaaa cgccacatag
      841 acaatcctac tgctgcgccg atcccaattg aaattaccat cagcatgatt ttcgatcgga
      901 ttctcccctc gctcaattct tcaacctgtt tgatcattac atgaatcgcc ggctcagcaa
      961 accctactaa gaaccccatt aaaaagccaa ggggaaccag tatataattg taaggcaatc
     1021 ccgcaatagc actgcctaaa tgctgtccta ccggtacata agccatattg actccgtgca
     1081 ggaaaaggat caacccaata gtcgttatca agaaaccttt cataatgtca aagaaacgct
     1141 ttttgcgcat acgaaaacta atgatattca ttaaaacaaa taggattacg ataggtgcca
     1201 ctgccaaaat gacttccatg gtaacgattt cgattccatc tatcacttgc tgccagaaac
     1261 tcattggaag atcaccccca aaatcatcac ggcaagaatc ggccctaacg aagcaatacc
     1321 gaccattcca aaactgtcat tttcattttt cttttgtttg atcatactag tcatccctcc
     1381 ggcaagggcc aagataaatg gaacagttac tggaccagta gtcactcccc ccgaatcaaa
     1441 agcaatcggc ataaattcgg aactggtgaa aaatgaggcg atcaaaacgc ctatgtagcc
     1501 tatagccatc aattgataat aggatagctt gaatacgacc cgaagcagcg caaaggctag
     1561 aaatactcca gtgccgacgc tgacgatccc aatcagcaac actctgccaa tctctccgtc
     1621 agatatatca tatacttgtt gtcccaaaac ttgcacggaa ggttcagcaa tcgtgatcac
     1681 gatcccgata gcaaatccca aactgaccaa aacggtcaaa cttttctttt tgatcatata
     1741 tttccctact agatctccga cttccatcat agaataatct gctccaaaca aaaagagcgt
     1801 cattccgatc atcatgattg ccgcaccaac cagaaatgaa gcgaggtctt ctccttctaa
     1861 cggtgcaaaa acaaacgtta aaatgacaat caggacagtc atcggcaata tagcagcaat
     1921 cacttcttta aaatttttta gcatgtatcc tcctccttta ctctttaagt ttaccttaaa
     1981 atgaaagagt tttcgagcac aaacttaaaa cgttatcatt taatttttct caacataaaa
     2041 taacacacgt cccaaaagta tctttttact tggaaaaatg actttgaagg aacgcgctta
     2101 ttgtgttttt ttattttaca gagccgatca ttccttgaac gaattgcttt tgcaaaacaa
     2161 aaaatacgat ggcagttggt aaagtagcga tgactaatcc agccatgatc attccatagt
     2221 ctggtgtata ggaagcaccc atagcagata aaacaagcgg aaccgtccgt ttatcaggag
     2281 attgcaaagc tactaagggc cataaataat tgttccagct actcatgaat gtgatgattg
     2341 cagccgcggc ataggtattt ttagccgtcg gcatatacac tctaaaaaaa atacttagtt
     2401 cttttagacc gtctaatcga gcggcttcta ctagatcttt tggaaaagat ttaacatttt
     2461 gtcgaaagaa gaagatcaaa aaagcagtgc taacagatgg caggatgaca accaagaaac
     2521 tgttgattcc caaaaccgtt ccgttcaatt gtgaaaacat tcgatataaa gggatcatca
     2581 atgccgcaaa aggaaccatc atcgatagca gtagaatatt gaaaattagg tcttttcgtt
     2641 tactttgata aatctcaaat ccatatcctg ccattgacga aatcagcaat gcaaacacag
     2701 tggtcaccaa agcgatcatc gctgaattcc aaagggaacg agtaaaattc agctcattgg
     2761 aaaacaaatt ttgaaaattg atgatcaagt tactgccaat ctttattttt cccgatgtga
     2821 tatccacggg tgtattcgtc attcccaaaa ccatccagac aaaagggaaa atggaaataa
     2881 atgataggat gcttaatgct gtatacttta atactgaccc aattgtcgaa cgaagttcta
     2941 atgcttgttt tttgacgttg gggcgttcga ttgtttcttt ttctaccata tttttcatcg
     3001 actttctcct cctactttcg tttggattat cgacaatatc acaataaagg aaacaataac
     3061 aaaggaaact gctgccgcat atccaaaatt aggcgtgaat ttataactca aattatagat
     3121 gtattgagat agggtggtcg ttgcatttgc tggtcccccg ccagtaatat tttgtacttc
     3181 atcaaaaagc tgcaatgtcc cgatcgtcga agtaatggaa gtaaacaaaa tgattggttt
     3241 taaatttggg atcgtcactt tgataaactg ctgccattta gaagcaccgt caatagatgc
     3301 tgcttcataa atctccgaat caatattttg catgcctgaa aggaaaaaaa tcatattgta
     3361 tccagtccaa cgccaagtga tcgaaagaat gatcagaact ctcgcccaaa ttggatgagt
     3421 caaccagcca atcggtgtgt caatcatacc aatgcccaac aacaagttgt ttacaaggcc
     3481 agattgagaa aatagacttt tcatgatcag agaataactg accatcgaag tgatacatgg
     3541 aagaaagata gcggttcgaa acaacccttt atatttcaac tttttatcat ttaaaaggtt
     3601 ggaaataaaa agtgctagaa acaacatgat cggtacttgt acaagtaaat acaggaatgt
     3661 attaaaaaaa gcttttttaa aagttgcatc tgtcaacatc cgctgataat tacctaaccc
     3721 gttaaatgtc atattgacag ggggaccact ttgaaaagac gtgatcaaag ccaaaaccat
     3781 cggaataaaa accatgattg atatcaaaat gactggcaag attaaaaaag catgcccgtt
     3841 tttgaataaa tctttctgct tcattctttt ccctccaatt ctatcattga ttatctacat
     3901 gaaaagacaa gaaagacaat atctgtaagg cagacgtgat cttttttgtc ttttcgtttg
     3961 tctgatgaaa aagcgaagaa attcactttt ttaccaccga tcaattagcc aattgtgctt
     4021 ctacttgttt ttgtgtatct gctagtacct tgtctgtctc ttctccattg atgattcgat
     4081 gaagagcttc agctacgact gcttcaattg cataggtctc atgtccgtaa ttcacttctg
     4141 gtatttcaga tgtccatgtt gaaaaatcgt caaagatctt ttgaccactg taaaattcag
     4201 aagcttcttg ataagcttct tgttcattcg cgctaagcat cgtagatact agtccgattt
     4261 ctttcgcaag agtgcccatc agttcttcat tcgaagcaaa cgttgcttct aggaaatctt
     4321 tcgctgaatc ttctccagct actcctttga tcacatacca gccagcgccg ccaaggttag
     4381 aagcttgcgc tttttgcaga ttttctggaa gtgcaggtat tggtgcaatc ttccattttc
     4441 cagattggtt ttctgctcct tggatcgttg aagaatacca agcgccagtt gggctagaag
     4501 ctactgctcc tgattgtatc gcagttactc cagcattcca gtcagaggtt tgttcgacta
     4561 gatcttcttt caaaagagtg gcaaacaatt caagaccata ctttaaagat gcattgtctt
     4621 ggatcgtgac tgtctcccca tcttcagatg tataccattc tcctgcctgt tgcataatca
     4681 tccttactcg accaaggtca gaaggattga cttcagcgat tttctttccc gtcttttctt
     4741 tgacatctcg cgctacttga atatactcgt cccacttcag attctccata tcctcttctg
     4801 tataacctgc ttctgccatc agatccgtac gatagaagtt tgctgttacg cccgaatcga
     4861 aaggaacacc gtatatttta tctcctactt tgtttaccgc aaatttatac ggtgcaaaat
     4921 gatcttcctt tacaatagca gacaaattag cgaatgcatc tggataagaa gtcaaatagc
     4981 cttgtatcct ataatcttca atcaagacga tattgggcaa tccttctgta ttcccgctcg
     5041 ctaaagcggt gttcagtttc tgaacgatat catcctgaga catcgtaaca acttctatct
     5101 ctgtttcttc gttctcataa actttttttg cttcattcac tgctttgata ttgaatgtct
     5161 catcccacgc ccagacagtg actttttctt ctttgtctgc tgtactacta gcacttccgc
     5221 atcctgttaa gaaacctgat gctgccgcta aacaaacgac tccagcaatt aactttctga
     5281 tgttcatttt tatccgctcc ttttccatcc attagttgtt tacgtttgct acattcatca
     5341 taccaaaatt tgaaaacggt ttctttatta ttcagatact tcaaaaattt ctcctaaaat
     5401 aataaaaaaa tacaaatcaa ctatgacaaa aggaataatc ttagcatcta gaccaaagat
     5461 cgaaatggtg aggtttcttt aaaataaaaa tatccttgca ttttctccta gaaataagta
     5521 tttttaaatt acaagactat tcttaaggcg ggtaaaaaaa tgccaatacg cgtaattctt
     5581 atcgatgatg aacagacgat tttagatgga atgaaacagt tgatcaattg gtctgagcac
     5641 gggtttcaat taatagggac tttttccagt ccgctgaaag cgcttgattt ttctttggta
     5701 catgcaatcg atatcgtggt cacagatgta atgatgcctg aattaaatgg aatcgaatta
     5761 tcgcggctat taaaacagca acaacctgaa acacagatcc ttatcctctc tagctatgat
     5821 gaatttgata tggttaaaga ttcttttaaa gaaggcgtcg ctgattatct tttaaagcct
     5881 cgattgagtc ctgatttttt tctaaattcc ttaactgccg tttcacaaaa aatcaaaaaa
     5941 aagcccaaca aaagcccaat caccaatcgt tatgagcaga tctgcgagaa tctttcacag
     6001 cagattgctg gacaaactcg tccattgctt gaccctcttc tttttgaaca caccttgttt
     6061 ttctgttata taccgaaaaa cggccattca gttcaaaaca aggctctaaa caaaaacagt
     6121 cgatcaccca ttcattgatg gatagcgacg attcgtttac tatttatcct gtttctacct
     6181 tttcggaaga atccggctat gtaatcaata ctgattccaa agatacactt aggaagtttg
     6241 ttcaaaagtt tcgtcaagaa gtttttccag gtgatttttt tgttgtttct gattcttttg
     6301 caatccaaga tatcaatgaa atattccaag agatcagaaa ttgcaccaaa ggacaacgtt
     6361 tctatcataa gggaaagaac cttgcttatc agcatgaact tcttccgtta ttaaccaacc
     6421 acgagcagca agcaagtagt tatttaacaa aaatcgttca taaagactac gctttagctg
     6481 tcaaagaact tgagaatttc ttcacgacga tattggaaca gcctattgat ccagcttatt
     6541 tgaaacatga ggcaatcaat cgtttctatg cattactcaa cgctttggct gacgagcatg
     6601 ttgggaatga agaactttct ttgctcaaga tcgcccttcc ttctttatta gccgatgctg
     6661 agtatttcga agatttttct attcttgtca aagaaacact tgagaaactc acccactttt
     6721 ctgcaaatgc aaaaaaagac aatttcgata tcttgacttt gatctacgag ttcgtccagg
     6781 aaaattatga gaaagaaatc aatttacaac ttttatccga aaaatatcat ttttcatata
     6841 gctatctctc agccatcttt actgaaaaat ttggtatcaa cttttcaaaa tatctaaaaa
     6901 aagtccgtat cagtaaagcc aagctctttt tgacggaaac acggcttact ttgacggaaa
     6961 tctgtgagaa agtcggctat accgaactgg gttatttctc cagagttttt aaagaagaga
     7021 caggaatgac accttctcaa taccgtaaag gaacttttgt aaaatgagaa aaaaaatcaa
     7081 agagcatgaa ctctttgcca aacttctgtt cattttgttc atcggtcttt ttctgcaagc
     7141 catcgtcatt tctttgttta tttatcatcg atcaagggat gcctatatcc agctgttcaa
     7201 tcaatcgaat gatgtggtac taaaaaagat tcaatctgaa tttgaaagtt tgaatgatac
     7261 aatcgagaat acattagccg cttttgattc taatccagct gtcaaaagct atttttccaa
     7321 tgatccagcc caacatatgg aacagcttca gcaactaaga acaatccaaa aaatgaatga
     7381 ctctctttca aaaatccatc caatgatcga ttacgatgta ttgatctttg gagaaaatgg
     7441 aagaactttt gtcggaaacg acatgctcac tgccgtatct gccgattctt tttttcaatc
     7501 tgcgattgct cagagagtga atgaacgtgc tgctgataca caaatgcttt ttgcacacca
     7561 cggattgact cttcgcgata agaaagcacc cagcgtcttc tttgtcaaaa aactgaaaaa
     7621 tacattgaat cacgtttacg gatatgctgt tttatcgatc tcatccaagc aactagcgaa
     7681 tctttttcaa tctgtagtaa atcctgagat atcgaagatc tccttcatga acgaagaaca
     7741 gacgatcatc gcatcaaatg aagaaaatac aattgggagc cgatcaatga ttttcaaaaa
     7801 actaacagta ggtgaaacaa cgatttcaaa tggacagcag ttgacccgtc ttcctctgta
     7861 ccgccaggat tgtacattac tcagtcaagt cgatcttcat tctttatcta accaaatggg
     7921 cgtgattttt ccgattattt tgtataatat cgtgacactt gcttttgctg gctcattggt
     7981 atttatgtat ttggatcgac acacaaaatc gatctaccgc ttgattgatt cgctaaaacg
     8041 gctagaggaa gatggcttaa aagctactgt tcctgtacaa ggaagccatg aagtccaatt
     8101 gctagggaat acgatcaatc aattgctaag taatgctcag catcattatg aacagacgat
     8161 ccaagacgaa aagaagaaac gttcgcttga aatccaaact atgcaagcac agatacagcc
     8221 ccattttatt tataatacgc tgactagctt gaaatttttg atttggcaga aaaaaacaga
     8281 tgaagcagtt caaggaatcg acagtttcat tactttacta agaagtacga ttgggaaaaa
     8341 agaagaagtg attccagtca aggaagaaat caaaagcgta caaagttaca tcgatctttt
     8401 gagtatgcga tatggcgaaa gaatctcggc taaaataatg attcctgatg aactgcagtc
     8461 attatctatg ccgaatatga tccttcaacc aattatcgaa aacgcctatc ttcatgcttt
     8521 tcacaataaa acgcaaggat tcatcacggt gtacggcaaa acaaaaaatc agcttctttt
     8581 attcgaagtg attgataacg gcgatggctt cgatactgaa aataccaaac aaaaaaatga
     8641 tcatttttca ggaatcggca tcagccatgt agatgagcgg atacgcctaa tgtatggcga
     8701 tgcatatggc ttaacgatcc aatcagtgat tgggacagga acgactgtaa cgatttgtct
     8761 accgcttctt cctaaaaatc aatcagaata tcaaaaataa agaatagcca agcgctgatt
     8821 tttttatttt aagttaaata aatcaatcag tattcttgac tttcatctac gcgtctcttc
     8881 tactgtcagc actggataac agcccaatat ttgactttct cctccgagca tcttgatttc
     8941 ttggagcgca ttctctacta atgtcattgg acggtccaac aacaaatcga tcacgaaaaa
     9001 atattctccc aagctagttt tcaatggtcg ggattcaatc ttacttaagt tgatttcccg
     9061 ccagccaaaa gctgccagca ttttatgaag catccctggc cgattagcag gtagtgtcaa
     9121 gatgacactc atttttactg gagctgactg ctggctggtc atctttcggt caccgacgat
     9181 ccaaaaacgt gtctggttca actcattatc ttggatattc tcagctaaga tctccaatcc
     9241 aacatgctgt gctgtttcta atgaagcaat cgctgctgcg tcttctttcg gatgttctgc
     9301 tacatacatt gctgcagcag ttgttgattc tgttgctact aaaggaacat taggataatg
     9361 cgtttctaaa aactgttgtg attgtgctag tgcttgtgga tgtgaaagga tttttgtaat
     9421 ctttgttgct ggattgccta aaagctgctg cttgatagga agaacgattt cacttttaac
     9481 ttcgacttct ggatgtttgg aaagaagatc gattgtgtga tgaacagctc cttccaatga
     9541 attctctaca ggaacgacag ccagatcgac ttgtttgcga aacaaagcgc gcaaacaaac
     9601 agggatggaa gcatacgcag tcaattgttc tgtaggaaat agctgacaag ccgcctgata
     9661 agtgaatgaa ctttctggcc ctagatagct gacgttcata tgcttttgat cctttctata
     9721 atggtatcga tgatcttttc tggtgtttga ttggtcgtgt tgattcgatg tgtcgcactt
     9781 tcttcatacc aagaaagtcg ttgtgccaat aatgctttta tttcttctct tgtttttcca
     9841 agagctaaag gtcgaataga cgtttcatct tgttggatac gatcaactag acagtccgct
     9901 tctgcctcta agtaaagaac aatcggctgt gtttttaaaa aacgccgatt ttctgcactt
     9961 tggacaatcc ctccaccagt cgcgataatt ttgtcttcat ttgacagctt tcttaaaata
    10021 tctgactcca cttttcgaaa gcttttttca ccgtaatagt gaaaatattc ggcaatcgac
    10081 aattggatat gttcctcaat ctttgtatct aaatctacat actctttttg taaacattga
    10141 gctaaccctt taccgattgt tgtttttcca gcgcccatga aaccaatcag caaaatgcta
    10201 gccatgagct tctcctcctg acacaagttc tgctaaatcg tcaaaaaatg ccggataaga
    10261 gattgaaaca gcttcagctc cttctagttc gcaaggtcca tcagctatca aagcagctac
    10321 ttgcagcatc ataccgattc gatgatcgcc tctgctggac acttttgcgc catgcagagg
    10381 tgtcggccca tggatgatca gaccgtctgc tgtcgctttt atctttgctc ccattttctg
    10441 taattcttct gccaccgcat caattctgtt ggtttctttt acttttaatt cttctgcgtc
    10501 tttgatgatc gtgattcctt ctgcttgagt agccactaaa gctagaatgg gcaattcatc
    10561 aatcagacgc ggaatgattt ctccaccaat cgttgctttc ttcaaatgac tcgtcttgac
    10621 acttaaatca gccgattggt tctgttcatt gtgattcgtc tgggtaatcg ctgcgcccat
    10681 ctcctccaac acatctaaga taccagttct tgttggattg accccgacat tttttaacaa
    10741 cagttggctt tccggaacaa gaagtcctgc tgctagaaag aaagcagctg atgaaatatc
    10801 acctggaaca gttatctgtt gaccagttaa tttttgcggt ccggtcacta tgatcgtttt
    10861 atcttccact gtgatccttc cgccaaattg acgaatcatt tcttccgtat ggttacggga
    10921 acgttctttt tcaacgatta cggatgtgcc ttctgcttgt aatgccgcaa acagcaaagc
    10981 agatttgact tgcgcactgg ctacaggcat attgtatcgg ataggtgata aagagcgggt
    11041 tcctgtcaca gtgattggta gatactggtc attttcttct ccttgcaagt cagcgcccat
    11101 ctcgcgtaaa ggttccatca ctcgccccat tggacgttta gacaacgaag catccccgaa
    11161 cagagtagtc gtaaaaggct gtccagccaa aatacccatc aataaacgta tggtcgtacc
    11221 ggagtttccc atgtcaattg attcggttgg ttggacaaat gagtggctac cgcgaccatg
    11281 aatcttgatc acctcttcct cttcttcgat ctcgacacct aattgttgga aggcatgtaa
    11341 agtactcaga caatcttctg cgcgcaaaaa attttgtata gtcgtcgttc cttcagcaat
    11401 tgctccaaac atgatgctgc gatgggaaat tgatttatct gctggtatcc taacagtccc
    11461 tcttaatcca tgtatttgtt gtaataattg catttatcca gcctcctatt tgatccagca
    11521 atgataactg gtattagaca taatgattct ttttgcttgt aacaaatcct gttggttttt
    11581 aaaactgatt tgcagtattc ctaaaatatc ttccctggtc tcttggatct tcaaattaat
    11641 caaagaaact cccgcttttc cgatcagtcc agttacttct gcaataacgc caggttcatc
    11701 tggcacatcg ataaataaat caaaaaaggc aggaatcgcg ccttgcttat ggacaggcaa
    11761 ctggtctcga ctttcttttg cccgattgaa aaaagaaaag atttccgatt ggttctcagt
    11821 catgatccat tctgctattt gtgtcatttc ttgttgccaa tctgccaatc gttccaataa
    11881 tatctgctta ttgctcaaca aaatatctgt ccacatgaca ggatctgagg aagcgatcct
    11941 agtgatatct cgaaagcctc ctgctgccag ttgtttggct cgcggatact cttgattgaa
    12001 ttgttctgcc tgattgacta agccagctgc cagaatatgt ggaaaatgac ttagcattcc
    12061 tgttatacgg tcatgttctc ttgctgtaag agttacatat tttgctttgg ttccgcaaaa
    12121 tagttcttgc agtttttcaa catctttttt cgtcttttcc aaaggtgtaa agatgtaata
    12181 ggcattttca aatagtttag catctgctgc ctgtatacct gatttatgag aaccagccat
    12241 tggatgtcca ccgacaaacc gaagattttt ctgctcagcg gatgtcacga cttgctgctt
    12301 cgtactgctg acatccgtaa tgatgacgtg atcagacaag ttgattcttt caaggacatc
    12361 aagataagct aaggttgtac ggacgggaac ggctaataag ataacgtctg cttgttctgc
    12421 acctgtttca aaatcacttg caacgtaatc aacgatctgc tgttctactg cttgctcacg
    12481 ggtttttgac tgactatccc aaccaactac tatcagttct ggattggtct gacgaataca
    12541 aagtgccata gaagcaccaa tcagtccaag ccccacgata aaaacatgtt gtgccatctt
    12601 atttgccccc tttttagtaa tgtttcaagt agtcacggta ctccttcact gcagctttca
    12661 actcaacaag cgaatcactt gggaattttg acaatagctc tgtcgctacc acagtggcta
    12721 caacattttc cgcaacgacg ctagctgcag gaactgctgt actatccgaa cgttctacac
    12781 ttgctttgta aggttctttt gtatcgatat caacactttg aagtggcttg tacaatgtag
    12841 gaatgggttt catgaccccg cgaatgacaa gtggttcgcc attggtcatt ccgccttcga
    12901 agccgcctag attattggta cgtcggaaat agcctctttc ttcttcccat aggatctcat
    12961 ccatcacttc tgagcctggt aatgaaccag aagtgaatcc gaccccaaat tctacgcctt
    13021 taaaggcatt gatactaacc acagcttgtg cgattttggc atctagcttc gtgtcccatt
    13081 gtacgtagct tcctaatcca gctggaacgc ctccaacgag cacttctaca attcctccga
    13141 tcgtatctcc atttttcttt gtctgatcga tcaaatcttt tattctttgc tcaactgctg
    13201 gatcaacaac tcgtacttct gacgcttcag aaatttcttt gatttcttga atcgagatat
    13261 ttccaggtat ttctgcttgg attccaccaa gacaaatcac gtgtcctgct acgatgatgt
    13321 caagttcttc cagaattttc ttggcaattg cgccgattgc tacacgcatg gtcgtctctc
    13381 ttgcagagga ccgttcaagc acattccgaa gatctcgatg atggtatttc ataccgccta
    13441 ccaaatccgc gtgtcctgga cgcggacgtg caactcgccg cagtgattgg tctttttcag
    13501 gcacttctgc tacagacatg acagttgtcc aatttttcca atctttattt tctaccacta
    13561 aagtgacagg tgaaccaatt gttttgccgt gacggatgcc agacgtgatc cgaacacgat
    13621 ctttttcgat cttcatcctt cctcctctgc cgtaaccagc ttgtctgcga gcgagttcgt
    13681 gattgatttc ttcttctgac agaggtaatc ctgctggtag tccttcaata atggctgtta
    13741 attctggtcc atgggattct cccgcagtca tataacgcat cttacagtcc tcctaagtaa
    13801 tctttgaatg tctctgtggg aacaggaacg atttttgctt gcccaatctt ttctagcaag
    13861 atgatattca attggcttcc tcttgctttc ttatcatggg ttatcgcgtg gaataattca
    13921 tctggatgac ttagatcgta cgtaatcggc agatggaatt tttgaagtat cctttctaaa
    13981 gcagctgtcg atccagttgg agacaagccg attttttctg catgagaagt gatggctacc
    14041 attccaatag ctactccttc tccatgactg actaccccat atcctgctgt attttcaatt
    14101 gcatggccaa ttgtatgacc aaaatttaaa ataagtcgat ttccttggtc aaagacatct
    14161 tcttccacta cttttcgttt gatttccaac gtagcagtaa tgattggaac agcatgttcc
    14221 agaagatctt tttcgtcttt cagctcgttc agctgtttcc aaagcgttgc atctgctata
    14281 gctgctgatt tgatgatttc agcgattcct tctcgtaaac gacgtaccgg caaagttttt
    14341 agaacctcag gatcaatcaa tacaccagct ggctggctaa atgttccgac aaggtttttc
    14401 gctttgtttg tattgactgc tgtcttcccg ccaatactag aatctacttg tgcaagtagc
    14461 gtagtcggaa tttgaacgaa atggatccca cgcatgaacg tcgaggccac gaatgctgct
    14521 agatcaccca ccattcctcc accaagtgca agtatcccat cgctgcgggt aaaatcatta
    14581 tctgctaaaa aatcgtataa ctcagctgct tgttctaaag atttactggt ctcacccgcc
    14641 ggtacgattg aaaggactgg ttcaaaatta gcttgtctca attggaacat gattttttct
    14701 gcatatagtg gagcaacgtt tgtatcagtg atcacagcta ctttttgagg tctccaaatc
    14761 gtttgaagcc atgatccagc ctgttctagt gcgccttttt caatatgaat atcgtatttt
    14821 tggttatcaa catgtacagt aagcattcgt ttcacttctt tcttttaaaa agctggaatt
    14881 aatcgagtgt ctgaatcttt tccattgctt ctttcatgat cgcaacttct ttcatcatgc
    14941 gtgagtaagt cttttcattc aacgattggg gaccgtctga ccaagcattt gcaggatcag
    15001 gatggatctc tacgatcatg ccatctgctc cgcttgccac acccgcacga gccattggtg
    15061 gtacaagttc ccaaattcct gtaccatgac ttggatcaac aatcacaggg aaatgagtta
    15121 attttttcat caatgggaca gcacttaaat caaacgtatt acgtgtcgct gtttcgtaag
    15181 tccgaatccc acgttcgata aaaatgacag gagatttgtc ttcgactgca atgtattctg
    15241 cagcattaag ccattcattg atcgtaccgg aaattccacg tttcaatcca acaggtttac
    15301 ctgttttacc gacagcagat agcagtttaa aattttgcat attacgtgcg ccaatctgta
    15361 ggatatcgct gtactcagcc accatatcga tatgcccttc atccatcact tcagtaatca
    15421 ctttcattcc aaattcatca gccgcttgac ggatgtattt caatccttct tcttctaaac
    15481 cttggaaagc atatggagaa gtacgtggtt tgaaggcacc acctcgcaga atttttgcgc
    15541 ccccggcttt ggccattcgc gcacattctc tgatttggtc taatccttcg atagaacaag
    15601 gaccagccat cgtcacgaaa ctgccgtcac caatttttac tccgtctaca tccacgactg
    15661 tattgccagg atggaattct cgagaagtca atttgtatgt gtttaaaata cgcacagcat
    15721 tttctactcc ttcatagcgt gtaaatgctc cttcttgtaa acttcgcgta tcacctttta
    15781 gtccaatcac tactcgttct gttccttgat cgattgagac tttgaatcct tctttttcga
    15841 tatgatcgac gacttttttt atttgttctt ccgttgcttc ttttttcata attactatca
    15901 ttgtttttcc cgctttcttt tgtttactta tctatcttgg ctgattttct ctactttttc
    15961 aaggtggttt tacattaatt cctactaaat gaccgtgtcc tgttgttctc attttcgctc
    16021 cttctttcct acttatgacc cagataatca acaaaaaaag atgtccctgt aggaaattgc
    16081 ctctacagtg aacatcttca acgagcctta aaaagcgctg caaaaatata aaatagccac
    16141 tatagatgaa tttctctacg tggctgtaag tcgtgtttaa aaatctttag ccatcataga
    16201 tacaaacctg gtttgtttgt acccacgaag tcatgaatac aaacctatct aaaatagcta
    16261 aagtaatacg tattcagttt tgtaagtttt ctagatttca taaaaaatcg ctccaaactg
    16321 atgtaagaat tgtacctagt atagacataa aaaaatcgac tgtcaatcat ttttatgaaa
    16381 ctttttttaa ttttctgata gttaactttg tttttcagaa aaa
//
LOCUS       DAZKNM010000051        16050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000051 DAZKNM010000000
VERSION     DAZKNM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.051"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..878
                     /locus_tag="ACG8M9_001317"
     CDS             210..878
                     /locus_tag="ACG8M9_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0378103.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1078..1398)
                     /locus_tag="ACG8M9_001318"
     CDS             complement(1078..1398)
                     /locus_tag="ACG8M9_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378104.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1435..2817)
                     /locus_tag="ACG8M9_001319"
     CDS             complement(1435..2817)
                     /locus_tag="ACG8M9_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0378105.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2973..4160)
                     /locus_tag="ACG8M9_001320"
     CDS             complement(2973..4160)
                     /locus_tag="ACG8M9_001320"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0378106.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4167..5198)
                     /locus_tag="ACG8M9_001321"
     CDS             complement(4167..5198)
                     /locus_tag="ACG8M9_001321"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0378107.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5214..6668)
                     /locus_tag="ACG8M9_001322"
     CDS             complement(5214..6668)
                     /locus_tag="ACG8M9_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0378108.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6843..7748
                     /locus_tag="ACG8M9_001323"
     CDS             6843..7748
                     /locus_tag="ACG8M9_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0378109.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7839..8516
                     /locus_tag="ACG8M9_001324"
     CDS             7839..8516
                     /locus_tag="ACG8M9_001324"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0378110.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8513..8683
                     /locus_tag="ACG8M9_001325"
     CDS             8513..8683
                     /locus_tag="ACG8M9_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378111.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8797..9693
                     /locus_tag="ACG8M9_001326"
     CDS             8797..9693
                     /locus_tag="ACG8M9_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0378112.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9748..11118)
                     /locus_tag="ACG8M9_001327"
     CDS             complement(9748..11118)
                     /locus_tag="ACG8M9_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0378113.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11356..12102
                     /locus_tag="ACG8M9_001328"
     CDS             11356..12102
                     /locus_tag="ACG8M9_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378114.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12099..12938
                     /locus_tag="ACG8M9_001329"
     CDS             12099..12938
                     /locus_tag="ACG8M9_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0378115.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12939..13889
                     /locus_tag="ACG8M9_001330"
     CDS             12939..13889
                     /locus_tag="ACG8M9_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0378116.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14030..14782
                     /locus_tag="ACG8M9_001331"
     CDS             14030..14782
                     /locus_tag="ACG8M9_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378117.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14801..15952
                     /gene="queG"
                     /locus_tag="ACG8M9_001332"
     CDS             14801..15952
                     /gene="queG"
                     /locus_tag="ACG8M9_001332"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0378118.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5204 a         2998 c         3230 g         4618 t
ORIGIN      
        1 acaaccgtaa cggttgtgta aaaatatcat tttagagcat aaaaaaaagg agtggatttc
       61 tccactccaa aaaagtctaa cttttggggg tcactacagt gtcacagtcc tttttttagt
      121 aaaaggatac atttcttcct tgtatattcc atgcgtgttc gtgagaaagc atatataatt
      181 taattaacag atagttaggg ggagaaaaaa tgattttttc aattacaatg aaactggtgt
      241 tcgggttgat cggtttatta ttagtggtac gtttgcttgg gaagaaatcc atgtcagaaa
      301 tcacaccgtt tgatttagtc tatacgcttg ttttaggtgg gatcttagaa gaatcaacct
      361 atgatgacaa tgtccatgta gggcatgtcc tatttgccat tgcgctttgg gcagtaatga
      421 tttatggtat cgaacgtatc gtccaaaaaa atgaaaaggt caatcgttgg gtcaaaggcg
      481 agccatctgt tctaatcaaa gacggcgtca tcaacatgac agagttgaca aataaccata
      541 tcgagatgga gcagttgcga gcaatcttaa gacagcaaga atgctttttt cttgaaaatg
      601 ccaaacatgt tatcctagag aatgccggtc aaatgagtgt attgaaaaaa tcagatgaag
      661 acaaagcttt atcgatcctg cttgtagatg aagggcaaat tcagcataaa gtgcttcagt
      721 ccaatcaatt gactgaagca tggcttatgg agaacttgaa gaaagaaggc tatgcagatg
      781 taaaacagct gatttatgtt gaatggtcag aagaaaaagg gctctacata ctaaataaag
      841 aagacgtcat gcatgaatct taccgaatag atggatagat agacagaggt tgtgaaatca
      901 gcgccttggc ttgttactca aaactaagcg actttttcac aacctctgat atctaatata
      961 cgttgttcca gaagttgctt cttcgaaaaa taagccaaaa ttccacaaaa atttaagaag
     1021 caattttcga aaaatccttt cttatttttt gaagttgatc acttctgtca caacctctca
     1081 tgtcacaaac catttttttg tttcctttaa ctcaatatac cgtacgtctt cccacaatat
     1141 cggctgatgg tcaatcataa agtaatcttc ataggcttca ccaatgaagt gcccttcgac
     1201 ctcatccaag agtcttccaa attcatcttt taaattgagc tgaatgatta ctgctttacg
     1261 agataaaaag gcttctgtca gtatactatc gatttcttgc aatgtcattt gttgtttagg
     1321 aagaggctgt ttgcgtgctt cttcttcatt tttttgatac cgttgactag ttcttccatc
     1381 gcaaaagcag tcccccattt taatccaaat ggccgatctc gatactcgtt atagtcaatg
     1441 aatttttttg ctgtccgtct catcttttcc accttctaat ccactcatac cacctgcatg
     1501 cccgccaacg agagaactac gtgcgatggc gcgtcctcct tgcaagagac tatgcgcatg
     1561 gatgattgat tgaaagccgt atttttttct aatcgtatcg acaacaaaat caactttcgc
     1621 ttcatttatc tgcttttcag gatcttcaaa aaggttaagc tgtaaacctg tcgcgtatac
     1681 gaggtcggaa cagttcacgc ctacacgccg aatatcctgg tagtggtaat gctggtggaa
     1741 aatggtcaat aaagcatctg ctagatctct gctagcattt gtcgcttcga ttttcatttg
     1801 ctggtgaaac ccggtcttgc catttggatc aagatagcct aaagagtatc ctacccataa
     1861 gctgactact tgtgtttttg ctgcagatcg tctcaagcga gtgccgactt ggtcggccat
     1921 ttctttgata acgatctcga tttcttctcg tcttgtataa tctctatcta ggatttgact
     1981 atttccgata ctttttgatt ttggtgtata ttcttgtccc aaaaaacttc gatcgatgcc
     2041 ccaagcatgg gcataaagct gagtaccgat gacccctagc ttgcttttca agtgataata
     2101 atccgcgtga gctaaatcat aaacagaatg tatacccatt tgtttcagac gttttgctgt
     2161 tcgtttgccg attccccaaa agtcagtgat atctgagatt ttccataact tttctggtac
     2221 atcttcataa cgccattcag caaacatttg ctttccgtct tttgagccat tgtctaaggc
     2281 tagtttagca agtagcgggt tatcgccaat tcctatagtc gtgtagattc cggtctgctc
     2341 aaacacatct ttatggatca gtttagccat gtcgtacgca tttgttttac caaagagtgc
     2401 catgctattt gttacatcaa ggaagctttc atcgacggaa tagacatgat ggttcgcttc
     2461 gtctgcatat tttttataga tagaattgat ttctctgttc ttttccatat aataggccat
     2521 ccttggggca acaatatata aatcacttgg ataggggaag ggaagatctc tggcacggct
     2581 gatattggtg atgccgtaag ctttttttgc tgcaggactg gaagccaaga ttaatccgct
     2641 accccgtgtt tctgtcgaat cggacggata ggacatgact actaatttag ttgtaagggg
     2701 atcaagatcc ttttcaacgc actctacgga ggcgtaaaaa gatttgcagt caatacataa
     2761 aatatctctt gaaggttctt tttcataaac aaataccgga ttttgattcg cccacataac
     2821 aatcacctct tcaacacaat tagaacacac gttctctttc cgtgtcaata aaaaacagca
     2881 attttcaaat acgagcaaaa ggctcacatt tggaaattgc tgagaggtta tttttatcta
     2941 tttgttttta gctgagatcg ttgatttttt tatcaaacat gaacagcttc tggatcgatc
     3001 tttgttgtcc catttccaat gtgtgcaaca taaaattccg gagcatagcc gacaactttc
     3061 atgtattcat catagacatt gtttttgaag gcaggtatat tttcttcttt gacaagagca
     3121 atcgcacagc caccaaatcc agctcctgtc attcgtgcac ctaagacacc ttcttgtttt
     3181 tgagcagcat ctactagtgt atctaattcg atcccagtta cttcataatc atagcgaaga
     3241 gagtggtgag aatcatttaa cagttgacca aatgcttcta agttcccagc ttctaattca
     3301 gcttttgcct tcaaagtccg ctgattttca gaaacggcat ggcgagctct gcgtatcaat
     3361 gtatcatcgc cgatttggtc aatagttgat tcaaattctt cttcatctaa gtctcctaaa
     3421 gaagagatag gcaaagtttt ttgcaaacgt gccaaggctt cttcgcattc gctacggcgt
     3481 tcattgtatt ttgaatctgc caattcccgc cgcttattcg tattcatgat gacgattgca
     3541 taaccatcta gtttcactgg aaccatttcg tatttcagcg tatttgtatc taataagatt
     3601 gcatgatcta gctcaccgaa gccgatggca aactgatcca tgatacctga attgacaccg
     3661 atgaattcat tttctacttt tttacctgtt tggactagtt ccaagcgatc tgtttttaaa
     3721 tcaaacagat cttctaatac aacacctact aataattcta atgaagcgct actggaaagt
     3781 cctgcaccat tagggattgt tccttccact aataactcaa acccatgatc gattgagtgt
     3841 ccagcttctt ttaatttaag gaccattcct tttacgtaat ttgcccagtt tgctttttta
     3901 tcatattcta gctcatctaa agtaaaacta atgacaccga tatcgtcaaa gtttgtggaa
     3961 taaactaata ttttattatc gtctcgaggt gctgctacac cttgcgttcc ataagtgatg
     4021 gaagcgggga agacatggcc tccattgtaa tctgtgtgtt caccaatcaa attgatgcgt
     4081 cctggtgaga agtattgtgt agttttttta ggtccgaatg tttcggcaaa ttttgtgttt
     4141 aagcttcttt tgatttccat gatgagttat tcctttcttg ttttgatttt gaattctgtt
     4201 actgtttcaa atactgatcc ggcttttaaa tgaatcgaac caaatgctga gaagcgctct
     4261 gcacctggag ctgtttgagt ctcaaatgtg atgccaccgt gatgtgccag ttttcggttg
     4321 cgcatttctg gtgtttctgt gccgaaattt gcagtgaaaa taaccacact agaagcatct
     4381 gtagcaatat ctacttggat tcttttatcg gggctagtca agcgagcggc ttctttaccc
     4441 aagccggttt cttttaagaa aaatggatga tcgattccgt caacaagatt tttttgatcc
     4501 aaatcactag caaaaacgga cgctaatttt ttaggtattt gaaaatcgaa agcacttcct
     4561 gcagcatttt cttttacccc tataggaata ctatctgttc gcaaaggagc ataagcttcg
     4621 ctattgagcc aaagctcatg ttgatcgatt gactgagaag catctcccgt caagttgaag
     4681 taaacatgat ttgtcggatt gaataaagta tcttggtcac tggtgccgcg agaggtcact
     4741 cgccagatat tatcttttgt caaagtatag cggatttcca catccattgt acctggaaaa
     4801 ccatgttctc cgtcgggact tgtagtagta aaaatgacag aagcttcatt ttcgccattc
     4861 aaaatgacat aagaccattt ttttaattca aatccaggag caccaccatg cagattatgt
     4921 ccagtttttg gatctgtggc taattggtaa gtttttccat tgagagaaaa gcggccattt
     4981 tcgattctcc ctgcggtgcg accgatagaa gcaccgatat acggatcttt ttcgatatat
     5041 tcttcagcgg tgtcaaatcc caaaactaat tctcgatcat ttgattttaa actgacgatt
     5101 ctagcaccta aatcagagat agaaagtgct agcttgtttt tgttctccaa ggttatcaag
     5161 tgataacctt ggccaaaatc tttttctgta atcttcattt tcaagatcct ttcttaatgg
     5221 gaaggatcta ctgcatcgtg aagttcatct tccattgtat cagctggagc caatttgttt
     5281 tccaatttct caacgatttg tgcatgtttt ttctctgtca atgtaatctt ccaagcaaaa
     5341 ataatagcgg agaaaatcat caaagcagca ggagcataga atgcataagc tttgaaagtc
     5401 gttacgccat gagcagtgat atcagcagca gtagcattac cagtcatccc agaagcgata
     5461 gcgacaaacc ctacgatccc gttagacaaa gcaccagcga ttttatctag taaagggcga
     5521 attgatagag taacagcttc gttacggata ccatttttcc attgaccata ttcaacagag
     5581 tctgtgatcg tcatcaaagc ggataagaaa atcaattgtt gagggaagta gaacaatact
     5641 agcccgatga taacgattgg caaacttgaa cctgcgatcg taaagaaaaa gtaagaagaa
     5701 atcatcattg cgatccctcc aaggtaaacg tagcgacgag agatgacgcg tgtaagcatt
     5761 gggaatagtg gaacagctac taaaccaagt aaggcagcaa ctagaccaac gatccaaaat
     5821 tcagttggtt ggccaagtac atatttgaag aagtagaata atacggcagt tgttgctgca
     5881 ttggcaatcg cataagcgat ataagcaagg gcaagccaca tcaactgatc attttgagca
     5941 actgctttga acacatcttt gatcccagct ttttcatttt gctgacggat cgcagtatcg
     6001 acttcttttg tgcccaaggc agtgataatc gcagtaccgc ctgaaataac agcagcaatc
     6061 acaccgaacc agaaccagcc tgaagcgcct tcaatatgtt tatgagtaac aagataggta
     6121 aagaaagtaa cgatagggac aacgactaga gtagtaccat ttgcacctag agaagagccg
     6181 aaacgagcaa acgtagcaaa tttttcacgt tcctttgttt ccgtactcaa agctggaatc
     6241 attgaccaaa aagcgatatc cttaaatgaa taaaaactat ccaataagat gaaaacgatt
     6301 gcaaataata tgataaataa agtcgtatta tgagtagcta agccaaagaa attggtaaag
     6361 attgctacca agagtacgga actaacaacg ccgccgataa ctaaccatgg tttgaattta
     6421 ccgtattttg tacgagtatt atcgatgatg ccaccaatca aaggatcaaa aataatttca
     6481 actaagcgga tacctacaac taagcttgta actgtagcaa tcatttttgc attcgttgcc
     6541 ttatctgttc ctgcgaacat ctggcttgtc acgaatacca taaaataagt gcttaatgta
     6601 acatagaatg tgtcgtgtcc aaaagcgcct aaagcgtagg acacacgttg gatcattttg
     6661 cttttcataa tctgtattcc tctcctgttc aatatataat actaagtaaa ccggttacaa
     6721 cgaataacaa agatctttta atgacttaaa cttaacaaga aatcgcttcc tttttaatat
     6781 atttctgtga cttttattta actttttgtt gcattatact aaaggagtaa ggagggatag
     6841 acgtggagca atcgattttt ttaaaaaagg aacatcaaca agtagaaaaa gaattgtatt
     6901 ttgatttttg tggtttctct aaaacgatgc cgtttcattc ttttggtcct gctattcgca
     6961 atgattacat cctgcatgtc gtgatggaag gcaaaggatc ttatcatgtg aaaaatcaac
     7021 aattccaatt gaaaaaaggt gatctgttct tgattcgtcc aggagactcg acgttttact
     7081 tatcagatgg agaagatccg tggatgtatt gctggctttc atttggtggg agtgctgcag
     7141 aagaaatcat tcggcagtcg cttttcagag aagaccaata cacgatggtc tcttctgaca
     7201 tacaaaaata tatcgacatt attttagaat gcatgaaaga tacggaagaa accttggaaa
     7261 atgaattaca gttaacagca ttaacctacc gttttttatc agtcctttta aaagatggcg
     7321 gaaaggtcag cggtggtgta caaaaggttc attcctcttt agctgttgaa acagtcaaat
     7381 acatcgaggc acattatgct gaaaaattgt ctgttgagga aatcgcccag catttagcgg
     7441 tcaatcggag ccatttgtcg cgagtattca aaaatcatat gggtaccagt atcaaggaat
     7501 atttgatcgg ggtacggatc aatcgagggg cgtttcttct ttctctgacg gatgaatccg
     7561 tggaaagtat tgcctatcaa gtagggttca atagtctagt cgtatttagc cgaatgttca
     7621 aaaagagtac cggagaaacg gctacgaact atcgtaaacg tatgaaaaac gaaaatttta
     7681 agaatattgc tttggatacc ttgaaaaaac agttggaaaa acaaaccgca gtttcttgga
     7741 caacctgatt agtctggtta aaatgacaag catcggcaaa agaaacttgt ggcggttatg
     7801 ctacactgta aagcgaagac taaaaaggag gaaaagggat gcgtacactt atcatcatta
     7861 gccatccaac gatcgaagaa tctttaaatc agcaattttt caaagaagct tctgctggtt
     7921 taccagctac ttggcatcat ttggaatcaa gttatccaga cggaaaaata gatatagaga
     7981 ctgaactaca gctcttggcc cagcatgatc ggatcatttt ccagtttccg ttttactggt
     8041 atagttctcc tgctcattta aaaatttggc aagataatgt cttggaacga gcagcacatg
     8101 tattaaaagg gaaagaacta ggaatcgtat tgacaacagg tgtggcggaa aaagaatacc
     8161 aagctggagg aaaagaagaa tatacgattt ccgaattttt gaggccgtat cagcgaatcg
     8221 caaataaatt ccatatgacc tatcttccgc cattcgtttt agcacaattt atgtatttat
     8281 cccaggaaaa acgttgggag aagctaattg cttatcagca gtatctctca ttggaaggca
     8341 aaccatcgct gactcagcgg attgactggt ttattcaaag agtacaggaa aatcagaaaa
     8401 tgcaggaaga agaatctgaa aaacaagcat atatcgtaga agcactgact gaggcaaaag
     8461 agcagatcga agacttgacc tttacactgc aagaaatgaa aggaacttca ttatgacaaa
     8521 tgattggctg atccaacaat tagatgaatt aagcgaaaaa tcagaatatc aagaccgtgc
     8581 gctcttatat gaaataaaga aactgatggc tgagatagac gaacgaacaa tgcagctgca
     8641 aggagaaatc gacggacaat cttggaatca tctcaactgg tgaaattttc ttataaataa
     8701 caaagataaa aatgtgcaca gcgacaatta tttgctacaa taagaatatg aagcgtttga
     8761 taacgctatt atacataaaa attggtggtg aaaaaaatgt atgatgaaaa tgagtgggga
     8821 gaaaatgttc ggatgacccg aagaagacag caaaaacagg cagctcgtaa gcggatgatc
     8881 atgattgggg cagcagttgc cgtgtttttg atcatagcag gaggagttgc gggtctatat
     8941 tttcatggaa aaaattcgtc agaagcgaaa agtacgacca taacggaaac aacgacaaag
     9001 aaaacagaga cgaaacaaac aagtgagtca tcaactaaaa caaaagagac acaaacttct
     9061 gaagaaaaaa cagcaaatgg tttgccagag caacttcaag caatcgtaaa tgattattcg
     9121 aaaaagatcg aagaaaagac gcctgcattg attgaagaat accagacaga aattcaggga
     9181 aatcaagagg gaatagctgg tttatcagca gttgctaacc aaaaagcacg tgagcttcaa
     9241 gcaatctccg acgaagggat cagaaaatta cgtgcagcgt atcaggcagc agaaaataaa
     9301 gacggtgtag acttagatac tttgatcaat caattatcgg ctaattatac gaatcatgtc
     9361 gctaagattt ccgatattta tttgcagact agcgcctctc ttcaagcaga gtcaacaagc
     9421 acacaagaca caacatcttc tgattctgag acagcagagt cggtagaagc ttctgaaatg
     9481 caagctcgaa caacacaaga cacaacagat tcctctgaaa ctaataccaa cagcgcgact
     9541 tcacaggctg cgacaaccgt agttttggaa ggagaaggtc caaatcaaat agccgaacga
     9601 actggcgtac cagtagaaac gatcttgtcg ctgaatggga tgagcatgga gaattatttc
     9661 ctgactcccg gtgagacgct aaaattgaat tgataaaagc aataaaaaag aacgaagctt
     9721 ccactaaggt gaagcttcgt tcttttttta ttttccggtt cttttttcgc gtacaaagta
     9781 atggatcgtc atgtaaagca agaaaacgag tccaataatc aatgagattc tcgtttcagg
     9841 attgataaac atgaaaatca atacgatagc taaaaaagca agtgtcacat aattcgtata
     9901 aggagatagt ggcattttaa aaggatgatc atgcatttta tctgcgtgct tctttttgaa
     9961 gcgaatctcg ctgatcaaaa tcactaccca aggaaccata ccaggcaaaa tacttgagct
    10021 gtatacgaaa acgaaaatat tttctgactg gttgatgaac attggtagaa gataatttaa
    10081 gatcagacca attaagattc cacctgagat tgctacgaca ggataaaaag gcgcaccgtg
    10141 acgagatagt ttcagaaatt ttttagacat ctttccattt atggcaagtg tatagaccat
    10201 acgacttgcg ctgaaaatcc ctgaattgca acctgataat gccgctgtaa tcacgacaaa
    10261 attaatcagt cctgcagcaa atgtaatgcc gactttagaa aaagtttgta caaaaggtga
    10321 accaatctca ctcaattgat tccacggata gatactcaca atgataaaaa tagcaccaat
    10381 ataaaaaatc aagattcgac cgatagtgga ctggacagct tcaatgatcg tctttttagg
    10441 attctctgct tcaccagcag tcattccgat cagttcgatt ccttgatagg aagcaacaac
    10501 gatcgataat gcaaagaaaa atcctttgac gcctccagtg aagaatccac catttttcca
    10561 aagatttgaa atccctactg cctgaccatg attaccaaat ccaaataaga ttaccccaag
    10621 acctgcaatg atcatcaaaa tgatcgttat gactttgatg atcgaaaacc aaaattctaa
    10681 ttcgccaaac gccttgacag aaataagatt tgccagcata agaaacaaga cgacaatgat
    10741 tccgggaata attgccggca aatcaggcca ccaataattc atataagatc ctacagcaat
    10801 cacttcactt attcctacga caatatattg aaaaatatta ctccaagcgg tcaagtagcc
    10861 agccacagga tggatgtact cgctcgcgta atttgcaaaa gaaccagtcg aaggatcaac
    10921 gtaaagcatt tcgcctaaag cacgcataat caaatacaaa aagaatcctg caatcgcata
    10981 ggctcccatc actgagggcc ctgtccaacg tatggttgag gaggcaccca taaacagacc
    11041 gacaccgatc gttccaccta gtgcgatcat ttggatatga cgggcactaa gacttcgagt
    11101 taacttttct tcttccatac aaaaactcct ttcgttgttg agtcaacaag aaaaaataat
    11161 caaacgtttc aacacatgtt tttcattatt aggttagcct tattgaactc ttgatgtgat
    11221 tctacagaaa attttcataa aatgcgagga aaattcgaaa aaaacttttt catcattcta
    11281 cttgaaaatg ctttccattt tagttaaact atatttaaat aaaaagttag gtaaacctaa
    11341 aaaggagtgt aagatttgga acgtacggcc attgtaattc aagacctttc agccgcttat
    11401 caagggaaaa ctgttttgaa aactatcaac ttgacgataa atccacaaaa aattaccggt
    11461 attatcggac caaacggtgc aggaaaatca acatttatga aatctttgct agagttgata
    11521 ccagctacta ctggtaaagt caccttttct ggacagtcgg taaactcagt ccgtaaaaaa
    11581 atagcctacg tggaacagcg aagtgaattg gatctttcct ttccaattga tgtattaggc
    11641 gttgtacttt taggaacata tccatcttta cgaattggac aaagacctgg gaaaaaagaa
    11701 aaagaacgtg caagacaagc tttgaaaaaa gtagggatgg aagaatatgc caaaagacag
    11761 atcagcgaac tatcgggtgg acagcttcag agagttttta ttgcaagagc tctagcccaa
    11821 ggagcagaat ggatcttttt agatgaacca ttcgtaggga ttgatgcgtt aagtgaacga
    11881 aagatctttg acatcttgca ggaattgaag aattcaggaa aaacgatttt gatcgtccat
    11941 catgatcttc ataaagtaga agaatatttc gatgaggtta ttcttttaaa taaacagctg
    12001 atcgcttccg gtccagtaca agaagtcttt acatcgaaga accttcaatt ggcctatggt
    12061 gagatcatca gacatttagt aaaaggagga gagaaaaaat gatccaatcc tttattgatg
    12121 gattaatgaa ttatcaattc ctgcaatacg ctttggtcac ttccatgctt gtaggacttg
    12181 cttctggagt catcggttct tttattattt tacgagggat gtctttgatg ggagacgcta
    12241 tctctcacgc agttcttcca ggtgtagcca tttcttatat gtttggtttt agttacatct
    12301 ttggagccac tgcttttggg atgctaacag cagcagtgat tggtttcgtg actcagcgga
    12361 gccgattgaa aaatgataca gcgattggaa ttgtctttag ctcctttttt gcattaggga
    12421 ttatcttgat ttcatatgcc cagagtgcga cagatttgta tcatatttta tttgggaatg
    12481 tcttagctgt tagggaaagc gatttgttgc tgacagcact cgttagtgga atcgtcttga
    12541 tatttgtctt tttcttttat aaagaattga agatcacatc attcgatccg acaatggcaa
    12601 aagcttatgg gctgaacaca tcgctgatcc attatctttt gatgttcttt ttgacattag
    12661 tggctgtagt cagtttacag acagtaggaa caatcttggt gattgccatg ttgatcacac
    12721 cagccgccac ggcttacttg ctaacgaacc atttactgaa aatgatcatt acagctgcag
    12781 gaatcggtat gctaagtgca gttgtcggtg tgtttttcag ttatagttac aattggccat
    12841 caggagctac gatcgtgtta gcatgtaccg cattttttat ccttgctttt ttattttctc
    12901 caacaaaagg aattttattt cagaaaggaa gcagatagat gaaaacaaaa aaatcactct
    12961 ttttaatctt ggcagttagt ttcttggtat tagccggttg cggaaagcag gcttctgacc
    13021 aagcggatga aggaagtaaa gaaaagttgt cagtcgtggc taccaattcg atcttggcgg
    13081 acatggcaaa agaggtcgga acagatcaaa tagatatcca cagtatcgta ccagtcggaa
    13141 cagatccgca tgaatatgaa gtactaccag aagacatcaa aaaggcaagc gatgcagatg
    13201 ttattttata caacggtttg aatcttgaaa caggtaacag ctggttcgat aacttgatgg
    13261 aaacggctaa aaaagaagaa ggaaaagatt attttgcagt tagcaaaaat gtagaacctc
    13321 tttatttaac tagcggtgaa gaacatacaa aagcagatcc ccatgcttgg ctagacctat
    13381 ctaacggaat aaaatatgtg gaggaaatcg cacgtatatt ctctgaaaaa gatgcagaaa
    13441 atgcgacact ctataaaaaa aatgcagaag catatgtgga aaaactaaaa gaattagata
    13501 cacaagcgaa ggaaagtttt gcttctatcg aagagaacaa aaaattatta gtaacaagtg
    13561 aaggggcatt caagtatttt tcacgagcat atgatttgcc agcagcttat atatgggaga
    13621 tcaatacaga aagtcaaggt acgcctgatc aaatgaaagc gatcattgat cagataagag
    13681 caaaagaagt accggtttta ttcgtggaaa ccagtgtaga ctcaagaagc atggaacggg
    13741 tagcaaaaga aacgggtttg aaaatctatg ataaactgtt cactgattcc atagcaaaag
    13801 aaggagaaca aggagattct tactatcaga tgatgaaatg gaatattgaa acgatccatg
    13861 aaggtttgtc acaaacaaaa gaaagctgaa aagtgcaagg tctagcttga ggagcagacc
    13921 ttgcattttt tataagagat atgaaaaaaa attatgctgt ttttcgtcgt acagatacct
    13981 tttttgaaga aattccgtta tagttagagt aaggaaggaa ggtgtggcta tgaattttac
    14041 acgtaaagat aaatatacga ttaaagaaga agtctttcga ttgatcagcc agcaggaaaa
    14101 taccgacaag gatcaagacc ttcaatttct tgaagcagaa ttagctaaat tattcatagc
    14161 aaatcaaaat gagacaacag aaccagatga agagctgtac ataggcaaat ggttgtttga
    14221 tgaatttcag cctttagatg aaaataccgg tatcggttat tttgtgttcc tttctccagt
    14281 cggcttcaca cctggcgtat tagttaaaca aaacgacgaa ataaccgtca aagcatggcc
    14341 tgcattattt tcagtcatca aagcagaagg agtaatcaaa aagatccagt cggcctatta
    14401 taaaagtcaa tttattgcca acagcaaatt aatgaatatg ttttataatc gtctagtaga
    14461 aattgctagg cctgggaaaa tcccgcaaac attcacgtac acacagcaaa aaaatctcta
    14521 tgaagaagta ttgtattccc gcgatctgtc tgagttttcg ccatgggaag aggtaaggct
    14581 ccatgaactg ttaagtacaa aaccagtttc attgaatttt tttaatgcat tagataacaa
    14641 aagaaaatta atttcaacaa attattggga agtatttgct gcaagaaaaa aatggatgga
    14701 agaaaagaag attgccgatc agcagatcca gtttgtccag ctgaaaaaag aaaaactata
    14761 ttatgggtta tacagccgtt aacagcaagc gaggaagacc atgacattaa aagaagaaat
    14821 catacaagaa agcaaacgat taggaattga caaaatcggg ttcaccacgg cggaaccttt
    14881 tgattcatta aaagaatcgt tggaagaaca aaaagcagct ggccatactt ctggctttga
    14941 acatccaaat atcgatgagc gtctttatcc agaaatgaca tttgatcagc cgaaatcgat
    15001 catttccatt gcacttgcgt atcctactag gatccatgaa gaagtgccta gggatgaaaa
    15061 acgcgggcag tttgctcgtg cttcttgggg gatcgattac catgatatac tcagagaccg
    15121 tttgaaccga ttgatcgaat tcatccaaac aagagcagaa aaatggcaaa aagaagagga
    15181 atggcgtcta gctccgcaag tagatactgg agaactgatc gatgtcgctg tagcacagcg
    15241 agctggttta ggatttattg gacgaaacgg attgttgatc actgaagagt tcggttcatt
    15301 tgtctatctt ggtgagatcg ttactaatat tgtatttgag cctgataaac caggagaatt
    15361 tggttgcggt gactgtatgc gctgcgtgac tgcttgtccg acaaaagcat tgcttggtga
    15421 cggaagaatg aatgcccagc gatgtttgtc ttatcaaact cagacaaaag ggatgatgcc
    15481 ggaagaatat cgtaaaaaaa tgcgaaatgt catttatgga tgtgatatct gccagttagt
    15541 ctgcccttac aatcaaggaa aagattttca ttttcatgaa gagatggaac caaaagtaga
    15601 cgaaatatat ccaaaattaa agccaatgct ttcgatgtct aataaagact tcaaacagca
    15661 atttgggcat ttagctggtt catggcgtgg gaagaaacct ttgcagagaa acgcattgat
    15721 tgctttagct aatctaggtg atcgaacggc attgcctgag atttcagaat gtttaacaga
    15781 tgtccgccct gtcatcagag gaacagcagc ttgggctatt ggacgtctag gaacaaaaga
    15841 accggaaaaa tggcttgatg tattagatga aatattagct aaagaaacag aagaagtcgt
    15901 tataaacgaa ttggaacatg cccagaaatt attgcggaga aagttaaaat aaaaaaatag
    15961 ttaacattat tcaaagtttt tgtccagtaa ttgttgtaac aattactggt ttttttgtat
    16021 tttttacatt tttttaagag aatttgataa
//
LOCUS       DAZKNM010000052        15817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000052 DAZKNM010000000
VERSION     DAZKNM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.052"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1134
                     /locus_tag="ACG8M9_001333"
     CDS             109..1134
                     /locus_tag="ACG8M9_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0378119.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(1729..5382)
                     /gene="rpoC"
                     /locus_tag="ACG8M9_001334"
     CDS             complement(1729..5382)
                     /gene="rpoC"
                     /locus_tag="ACG8M9_001334"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0378120.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(5461..9078)
                     /gene="rpoB"
                     /locus_tag="ACG8M9_001335"
     CDS             complement(5461..9078)
                     /gene="rpoB"
                     /locus_tag="ACG8M9_001335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0378121.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            9806..10774
                     /locus_tag="ACG8M9_001336"
     CDS             9806..10774
                     /locus_tag="ACG8M9_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0378122.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            10792..11697
                     /locus_tag="ACG8M9_001337"
     CDS             10792..11697
                     /locus_tag="ACG8M9_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378123.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            11694..12500
                     /locus_tag="ACG8M9_001338"
     CDS             11694..12500
                     /locus_tag="ACG8M9_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378124.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            12657..13625
                     /locus_tag="ACG8M9_001339"
     CDS             12657..13625
                     /locus_tag="ACG8M9_001339"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0378125.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(13942..14466)
                     /locus_tag="ACG8M9_001340"
     CDS             complement(13942..14466)
                     /locus_tag="ACG8M9_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0378126.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            14753..>15817
                     /gene="scm"
                     /locus_tag="ACG8M9_001341"
     CDS             14753..>15817
                     /gene="scm"
                     /locus_tag="ACG8M9_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0378127.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4569 a         3371 c         3060 g         4817 t
ORIGIN      
        1 atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt
       61 actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact
      121 atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat
      181 aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg
      241 aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta
      301 gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt
      361 caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa
      421 acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga
      481 attattattt taagctctac gatggatgaa acacttcttt ttgaattgga tcaatacaat
      541 attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac
      601 acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt
      661 catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt
      721 taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc
      781 ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga
      841 ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa
      901 caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg
      961 tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc
     1021 aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc
     1081 attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga
     1141 attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact
     1201 catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta
     1261 gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa
     1321 ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc
     1381 ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag aataacagac
     1441 atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa
     1501 aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact
     1561 tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg
     1621 atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca
     1681 aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg
     1741 attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt
     1801 ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc
     1861 agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt
     1921 accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa
     1981 gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc
     2041 aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa
     2101 taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat
     2161 ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac
     2221 acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa
     2281 ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc
     2341 tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt
     2401 gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt
     2461 tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg
     2521 aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt
     2581 caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac
     2641 ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt
     2701 tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg
     2761 ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg
     2821 atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat
     2881 ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc
     2941 acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc
     3001 agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc
     3061 caagacggat agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt
     3121 aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg
     3181 agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt
     3241 ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc
     3301 atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt
     3361 ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc
     3421 ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc
     3481 tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa
     3541 cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa
     3601 gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa
     3661 ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc
     3721 tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga
     3781 atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac
     3841 cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat
     3901 atcttgggaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt tttgagcagc
     3961 taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat
     4021 ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc
     4081 gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc
     4141 ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc
     4201 ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac
     4261 aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca
     4321 ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg
     4381 tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga
     4441 tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa
     4501 tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt
     4561 caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt
     4621 caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg
     4681 aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc
     4741 ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa
     4801 ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat
     4861 cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata
     4921 ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac
     4981 atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc
     5041 catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat
     5101 atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca
     5161 aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt
     5221 aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg tacgatagtt
     5281 aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc
     5341 caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt
     5401 catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga
     5461 ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc
     5521 cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt
     5581 caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc
     5641 tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa
     5701 tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc
     5761 gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt
     5821 ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc
     5881 aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg
     5941 tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga
     6001 cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct
     6061 ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac
     6121 tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac
     6181 ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt
     6241 tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt
     6301 tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac
     6361 acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt
     6421 cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc
     6481 acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac
     6541 cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg
     6601 gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg
     6661 tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg
     6721 gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata
     6781 gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc
     6841 ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg
     6901 aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc
     6961 gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc
     7021 aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc
     7081 cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat
     7141 gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt
     7201 cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat caatagatac
     7261 ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc
     7321 attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta
     7381 gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat
     7441 aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg
     7501 gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac
     7561 ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt
     7621 gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact gagaagaacc
     7681 aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa
     7741 tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc
     7801 gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg
     7861 gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc
     7921 aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac
     7981 gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg
     8041 atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat
     8101 gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat
     8161 ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt
     8221 caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg
     8281 atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg
     8341 acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt
     8401 atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat
     8461 cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt
     8521 acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc
     8581 acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg
     8641 gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat
     8701 gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt
     8761 gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt
     8821 tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac
     8881 aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt caaacatttc
     8941 tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg
     9001 taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt
     9061 tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga
     9121 tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg
     9181 cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt
     9241 ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt
     9301 catcagatag tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc
     9361 ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa
     9421 ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat
     9481 tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata
     9541 acttttactt tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa
     9601 cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac
     9661 aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt
     9721 tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt
     9781 tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc
     9841 cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg
     9901 acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa
     9961 aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga
    10021 ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa
    10081 agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga
    10141 aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt
    10201 cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga
    10261 aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta
    10321 taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa
    10381 agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac
    10441 gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag
    10501 tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc
    10561 agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg
    10621 taaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc
    10681 tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg
    10741 catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat
    10801 ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt
    10861 ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt
    10921 cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct
    10981 tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca
    11041 aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc
    11101 acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat
    11161 tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt
    11221 gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt
    11281 cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg
    11341 aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc cttgcttgcg
    11401 caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct
    11461 tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact
    11521 gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt
    11581 gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg
    11641 cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg
    11701 acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc
    11761 atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg
    11821 gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc
    11881 agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga
    11941 aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag
    12001 aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca
    12061 aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa
    12121 atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat
    12181 tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg
    12241 accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc
    12301 tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga
    12361 tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca
    12421 cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc
    12481 aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg cccaccctct
    12541 tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa
    12601 actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt gatgccatga
    12661 caacgaaaga caaggtactt caattattga aaaagacacc agattttctt tccggagaaa
    12721 agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc aaagaattgg
    12781 aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg ccctctgacg
    12841 tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat ttgaaagtac
    12901 aaacgatgtt gacttctgaa tctacgatga aagatgtcaa actggcagca gctcaaggag
    12961 attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga cgttttggac
    13021 ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc catccagaca
    13081 aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt gtgaaagcca
    13141 ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc tacatggatg
    13201 gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc ggcaggatca
    13261 gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat tttccagctg
    13321 atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc acaaggaatc
    13381 agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca gaaaaagcgt
    13441 tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag
    13501 gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa
    13561 caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct atccgtacgc
    13621 aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt ttttctgaat
    13681 tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct ctagaaaaag
    13741 gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca atgcaaaaaa
    13801 caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct ttttcaatct
    13861 tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta ttttgccgta
    13921 agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg catagatcac
    13981 ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc ctcgtgtgac
    14041 tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca gccttcttaa
    14101 gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt ctatttgatt
    14161 agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt tatctgctat
    14221 cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag ttcctgctaa
    14281 atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag tcattgattt
    14341 gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag agacgccttc
    14401 aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca tatctaaata
    14461 atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt ctaggatacc
    14521 tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca tcatcaatag
    14581 tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa gcaacaatat
    14641 gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca gtttaagact
    14701 aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa
    14761 aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt atggtgtggc
    14821 cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg atctaactgg
    14881 taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa ataagacaac
    14941 tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa aaaataattt
    15001 tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac aaaaaactga
    15061 ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata aatttgtcgt
    15121 tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg gatccaggct
    15181 tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat atattgattt
    15241 cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga tcactccagc
    15301 taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg atttaactga
    15361 tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa cgatgaatac
    15421 gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca tcaacagttc
    15481 ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc atgaaacaga
    15541 agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg atttgacaat
    15601 cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca ttactcaaaa
    15661 ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca atcgttggga
    15721 tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa gcgacagtac
    15781 agatacatct accacaagtg atagctcaga ttcatca
//
LOCUS       DAZKNM010000053        15746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000053 DAZKNM010000000
VERSION     DAZKNM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.053"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..726)
                     /locus_tag="ACG8M9_001342"
     CDS             complement(28..726)
                     /locus_tag="ACG8M9_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0378128.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="ACG8M9_001343"
     CDS             complement(719..2071)
                     /locus_tag="ACG8M9_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0378129.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="ACG8M9_001344"
     CDS             complement(2294..3694)
                     /locus_tag="ACG8M9_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0378130.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="ACG8M9_001345"
     CDS             3855..4592
                     /locus_tag="ACG8M9_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0378131.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="ACG8M9_001346"
     CDS             4717..5196
                     /locus_tag="ACG8M9_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0378132.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="ACG8M9_001347"
     CDS             5193..5960
                     /locus_tag="ACG8M9_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0378133.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="ACG8M9_001348"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="ACG8M9_001348"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0378134.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="ACG8M9_001349"
     CDS             complement(6863..7465)
                     /locus_tag="ACG8M9_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0378135.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..7801
                     /locus_tag="ACG8M9_001350"
     CDS             7571..7801
                     /locus_tag="ACG8M9_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378136.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7792..8040
                     /locus_tag="ACG8M9_001351"
     CDS             7792..8040
                     /locus_tag="ACG8M9_001351"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378137.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8146..8730
                     /locus_tag="ACG8M9_001352"
     CDS             8146..8730
                     /locus_tag="ACG8M9_001352"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0378138.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="ACG8M9_001353"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="ACG8M9_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0378139.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="ACG8M9_001354"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="ACG8M9_001354"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0378140.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="ACG8M9_001355"
     CDS             10631..10810
                     /locus_tag="ACG8M9_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378141.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="ACG8M9_001356"
     CDS             10832..11857
                     /locus_tag="ACG8M9_001356"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0378142.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /gene="glyA"
                     /locus_tag="ACG8M9_001357"
     CDS             12032..13276
                     /gene="glyA"
                     /locus_tag="ACG8M9_001357"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0378143.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="ACG8M9_001358"
     CDS             13429..14376
                     /locus_tag="ACG8M9_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0378144.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="ACG8M9_001359"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="ACG8M9_001359"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0378145.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="ACG8M9_001360"
     CDS             complement(15278..15520)
                     /locus_tag="ACG8M9_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378146.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="ACG8M9_001361"
     tRNA            15641..15713
                     /locus_tag="ACG8M9_001361"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3196 g         4378 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg attttt
//
LOCUS       DAZKNM010000054        15655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000054 DAZKNM010000000
VERSION     DAZKNM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.054"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1448
                     /locus_tag="ACG8M9_001362"
     CDS             21..1448
                     /locus_tag="ACG8M9_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378147.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="ACG8M9_001363"
     CDS             complement(1540..1905)
                     /locus_tag="ACG8M9_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0378148.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="ACG8M9_001364"
     CDS             complement(1902..2267)
                     /locus_tag="ACG8M9_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0378149.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="ACG8M9_001365"
     CDS             complement(2459..2707)
                     /locus_tag="ACG8M9_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378150.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="ACG8M9_001366"
     CDS             2706..2834
                     /locus_tag="ACG8M9_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0378151.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="ACG8M9_001367"
     CDS             2891..4585
                     /locus_tag="ACG8M9_001367"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0378152.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4652..4858
                     /locus_tag="ACG8M9_001368"
     CDS             4652..4858
                     /locus_tag="ACG8M9_001368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378153.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="ACG8M9_001369"
     CDS             5236..6369
                     /locus_tag="ACG8M9_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0378154.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="ACG8M9_001370"
     CDS             6400..6720
                     /locus_tag="ACG8M9_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0378155.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="ACG8M9_001371"
     CDS             complement(6749..7213)
                     /locus_tag="ACG8M9_001371"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378156.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="ACG8M9_001372"
     CDS             complement(7345..8598)
                     /locus_tag="ACG8M9_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0378157.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="ACG8M9_001373"
     CDS             complement(8627..8944)
                     /locus_tag="ACG8M9_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0378158.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="ACG8M9_001374"
     CDS             complement(8970..9290)
                     /locus_tag="ACG8M9_001374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0378159.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="ACG8M9_001375"
     CDS             complement(9754..11169)
                     /locus_tag="ACG8M9_001375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0378160.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="ACG8M9_001376"
     CDS             complement(11186..12502)
                     /locus_tag="ACG8M9_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0378161.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="ACG8M9_001377"
     CDS             complement(12652..13350)
                     /locus_tag="ACG8M9_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0378162.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14628)
                     /locus_tag="ACG8M9_001378"
     CDS             complement(13639..14628)
                     /locus_tag="ACG8M9_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0378163.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="ACG8M9_001379"
     CDS             complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="ACG8M9_001379"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0378164.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4862 a         3177 c         2685 g         4931 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    14701 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    14761 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    14821 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    14881 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    14941 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    15001 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    15061 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    15121 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    15181 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    15241 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    15301 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    15361 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    15421 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    15481 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    15541 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    15601 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagag
//
LOCUS       DAZKNM010000055        15552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000055 DAZKNM010000000
VERSION     DAZKNM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.055"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..176
                     /locus_tag="ACG8M9_001380"
     CDS             9..176
                     /locus_tag="ACG8M9_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0378165.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(238..2049)
                     /locus_tag="ACG8M9_001381"
     CDS             complement(238..2049)
                     /locus_tag="ACG8M9_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0378166.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2046..2411)
                     /locus_tag="ACG8M9_001382"
     CDS             complement(2046..2411)
                     /locus_tag="ACG8M9_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0378167.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2574..2969)
                     /locus_tag="ACG8M9_001383"
     CDS             complement(2574..2969)
                     /locus_tag="ACG8M9_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0378168.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2987..3949)
                     /locus_tag="ACG8M9_001384"
     CDS             complement(2987..3949)
                     /locus_tag="ACG8M9_001384"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0378169.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3949..4161)
                     /locus_tag="ACG8M9_001385"
     CDS             complement(3949..4161)
                     /locus_tag="ACG8M9_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0378170.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4182..4880)
                     /locus_tag="ACG8M9_001386"
     CDS             complement(4182..4880)
                     /locus_tag="ACG8M9_001386"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0378171.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4900..5442)
                     /locus_tag="ACG8M9_001387"
     CDS             complement(4900..5442)
                     /locus_tag="ACG8M9_001387"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0378172.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5574..6578)
                     /locus_tag="ACG8M9_001388"
     CDS             complement(5574..6578)
                     /locus_tag="ACG8M9_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0378173.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6575..7564)
                     /locus_tag="ACG8M9_001389"
     CDS             complement(6575..7564)
                     /locus_tag="ACG8M9_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0378174.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7561..8367)
                     /locus_tag="ACG8M9_001390"
     CDS             complement(7561..8367)
                     /locus_tag="ACG8M9_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378175.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8533..9489
                     /locus_tag="ACG8M9_001391"
     CDS             8533..9489
                     /locus_tag="ACG8M9_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0378176.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9566..10084)
                     /locus_tag="ACG8M9_001392"
     CDS             complement(9566..10084)
                     /locus_tag="ACG8M9_001392"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0378177.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10172..10321)
                     /gene="rpmG"
                     /locus_tag="ACG8M9_001393"
     CDS             complement(10172..10321)
                     /gene="rpmG"
                     /locus_tag="ACG8M9_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0378178.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10549..10995
                     /locus_tag="ACG8M9_001394"
     CDS             10549..10995
                     /locus_tag="ACG8M9_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0378179.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11190..13085)
                     /gene="asnB"
                     /locus_tag="ACG8M9_001395"
     CDS             complement(11190..13085)
                     /gene="asnB"
                     /locus_tag="ACG8M9_001395"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0378180.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13410..14384)
                     /gene="bsh"
                     /locus_tag="ACG8M9_001396"
     CDS             complement(13410..14384)
                     /gene="bsh"
                     /locus_tag="ACG8M9_001396"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0378181.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14718..14805)
                     /locus_tag="ACG8M9_001397"
     tRNA            complement(14718..14805)
                     /locus_tag="ACG8M9_001397"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14771..14773),aa:Ser,seq:cga)
     gene            complement(14950..15516)
                     /locus_tag="ACG8M9_001398"
     CDS             complement(14950..15516)
                     /locus_tag="ACG8M9_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0378182.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         4839 a         3263 c         2615 g         4835 t
ORIGIN      
        1 tggattcaat gaaaaaattt gtatctggaa tattagtagg aagtctggca acagccgctg
       61 ccgttgctgg tttggttgct tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag
      121 aagcaatgat cgaagaaaac cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat
      181 tatttaaaaa tacgcatgaa gactttctaa aaaacagata agtcatcatg cgtattttta
      241 tttgtcagtc aatataagtt gtgctatttt atctgcctgt ctcagattcc atggatctgt
      301 ataaggtcta gctactgctg cagttagcca atccacacct tcaacaggta ttccatagca
      361 ataaaagtca gactggattt cccctgaagc atcaatcacg cgattcgtac ctcgctcaac
      421 atctatagct tctgaatgat aacttcctgt attatctgaa aatgaaaaag ggcgaacaag
      481 tttgttttcc ttcaaatttt ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg
      541 caatcgagct tcaatcaaat agcgactgct gatttcttta tcttgtagag aaataacata
      601 gcggttgtgc ttttgctgaa tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc
      661 tatcagtgct gctaattctt tcgtccgaat gattggcgga ccgatcgtta aaaagctatt
      721 tagatgagta aaccatcccc atagtatttc tttgtattcc ttagcagaaa aaacgtgcca
      781 ttcaatagcc tgccggattg gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa
      841 tgctcctgtg caattgcctt tttccgcttc ttttatctgc ccttcaataa atattcggct
      901 agcttctgaa aagggctcat tctgataggc aagaggagtt tctaaatacg accaatccca
      961 tctataaggg attagtgctg gatgctttcc gagacactcc tctgaagaga gggagagaaa
     1021 atcatgctca aaatttttct ttggaatcgg caagtgatat tcttcgatga ttttcttgta
     1081 ataaaacaac tcaacgtctt ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc
     1141 cgactgatgc cattgctcca ggttttcatt tgttagtaac cgtggccatg ccattgctcc
     1201 gccttgcttt tgatttcttc ctcgagggta ataagggagc ccccttcttg atccactgta
     1261 tacaatcggt tccttgcctg atggatgata gaccagtttt cccttttcgt tctcgaagcg
     1321 ccctcctctt gatactgtaa acaatccgac ataatcaaaa aaacttaagc ctagacctct
     1381 taaaaagaca ttttctttag gcggtatctt cgaaagatct acatctgcag gattagaagg
     1441 aagctgatac agtaaaacat ttttctttgc gtagtctacc cacactcttt cttcttctgt
     1501 ctcatcattt tcccagtgac cgcttgctaa aacaagctga tcgatagaat agctttgctg
     1561 atctgtctgt aaaagataac catcttttct tttgcttact ttttttacta aagaataaac
     1621 aaactgaata gaagctggaa attcttcttg caactttgag aaaaaccagc gttgatagag
     1681 tccgtataaa catcgagtag attgctgatc tttttccaga cgattgattt cttctataat
     1741 tagttgctta tttttggtta ccgtctgttc gatataatct gtagcaggtc ctttactcca
     1801 ttgaaacaag ttgggtccct gtgttaccaa tcctccactg gacaatgttt catctgtaaa
     1861 aagcgtcact tgttgagaaa cagaattcat taataactcc ggtggctgat tcaaccgcca
     1921 aactcgacct ccaggaccat tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg
     1981 attattatgg agaatccttt ctgctacact taatcctcta ggtccggcac caatgatccc
     2041 tattttcatt tgcatgcctc catttcgata aattttttca ataaaacagc atggttctga
     2101 tgttcatttt ttgcaccata aacaaaagta atgactggat gttctttcat aagttgactg
     2161 agtttaaaaa tagcttgctt cttttcttct tcgaaaatca attcttgctg gtatttcttt
     2221 gcaaatgaag caaatcgttc tggtacatgg tcaaaagttt tgcgtgcttc attactaggt
     2281 gcaatttctt tcaaccacag atctaactgc gctttttctt ttgaaattcc tcgtggccag
     2341 aatctatcca cgagtattct atagccatcc tctttttcag catcaaggta aacacgtttt
     2401 aatttgatca tagttgtttc cttctttcta ccttttcgct tagcttatat atgtagataa
     2461 tagcacaaaa taaaaaaaat gaacggcaca actatccatt gcgccgttca tgtgatcttg
     2521 gtcaaaatct aatgattgat tcggttttta aagctgtcat acaatcatct gttttatact
     2581 tttttacttt tgatttttcc agaccatccg tcatatccac ccttaagtaa ataaatctct
     2641 tgataacctg ctttacgtaa tttatttgct gtacggatgc tcaaactttt cttttgatcg
     2701 taaatataga caggctggtc ttttcgaata gatcctaatg aatctttcaa aacagtgtaa
     2761 gggatgtttc ttgcacctaa aatatgccca gcatcaaaca catctttttc gcgaacatcg
     2821 attacttgtg cttttctcat tccttctcga aattcttctt ctgtcaaagt cgttgctgaa
     2881 cgtttggcca ttactcgtag atagacttcc caaaggatga ttgcaagcac aatcaatagc
     2941 aaaatgatat taattaccca taaaaccatg taatgcctcc gtttttttaa gcaaatttca
     3001 aggctaatga ggcagcacca atgacaccag cttcattacc aagctctgct aatttgattt
     3061 ttgttgattc tcttacttgc gggaaagtaa attctttaaa atatttctct acacggctac
     3121 gtaaaaattc tcctgctgcc gatactcctc cacctaacac gatacttgac ggatttaatg
     3181 tattccccaa gtttccgcaa gctaaaccta agtagaagca tacgcgatca acaaccatca
     3241 atgcaaacgg atcgtcttct tgagccattt caaagatatc tttacttgtt acttcttctc
     3301 cgttgtctag cattgctttc agttttgaat cccctgcgta ttcttctgcc aagaaacgac
     3361 ctaggcgtac cacacctgta gcgctagata cagtttctaa acatccgcgt ttaccgcaag
     3421 tacattcgaa cccttcagga tccacagtga tatgtccgat ttctcctcca gctccgctca
     3481 cgccgtgaat caaatttccg ccagcaacga tacctccgcc aacacctgtt cctagagtaa
     3541 cgaatacaac gtctggatcg ttttctccag cacctttcca tcgttcacct aaagcagcaa
     3601 cattcgcgtc attatcgata gcgaatttga ttcctgttcc tgcctcgatc agttttttca
     3661 caaattgaac ttctgtccag tttaagttat acgcaccaat aaccgttcca gcttcgctgt
     3721 ccacgcttcc tggggtaccc atgccgattc cgataaagtc ttcggctttc atattatata
     3781 aattcaaacg atgattgatt gattcaatga tttcaggaac gatatgctta ccatcatcca
     3841 aaatattggt atcgatgctc catttttgct gaatctctcc ttctggtgtt aaaattgcaa
     3901 attttgctgt tgttccacct aaatcaatcc caataatctt cttgtccatt acaaatttcc
     3961 tttctttcgg ctttcttcaa ttcggtgctc tctttttaat atgcgccacg ctgtcatgta
     4021 tgtatcatga tcaatcaaac ggctatcata cagattacgc aattcgatag tcattaattc
     4081 aatgtcatag atgcgtgcac ccacatagat aaagatgcca aattttttga acagctgctg
     4141 tacatcatat aagctctcca tctttaagca cttccttcat attatacaag taaaccatat
     4201 ttttttaaac ccagtaaaat acatattaca aaaagaaata caaaaactag tgtagaaaga
     4261 atcctttggt gtatcgaata gcttgtctgt tgtttcggta gacccaaaac attccctaac
     4321 actaatccgc caattagccc accgacatgt ccccaaacat ctacagaggt atccagaaga
     4381 ccaaaaacaa atgtcaacac aataaataaa agaaaatgtc tgactagctg ctggatcgct
     4441 gggttatgtt taaaatgaaa ccctaagata aacaacgcac cgaacaaacc gaatatcgaa
     4501 gtacttgcac caccagacag aacattcgct tcgttaaaag caaagcttgc tgtatttccc
     4561 attatcccgc tgaacatata aatcaagaaa aaacgccaat ggccatagat tgcctcgatt
     4621 tgctgcccca taaaatacag tatcagtgag ttcattacga aatgcatcag tccaaaatgg
     4681 atgaagattg gtgtcactaa acgccaccat tcattgaagt gaacaagata aggaccatac
     4741 attccggcat aataagggat ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct
     4801 gtctgtatcc ctaaaaaagc ataggtccat aacggtcgtc ttaaccatac acgcaatttt
     4861 atttgctgtt gataattcat gaaaacctcc catttcattt tagtcaataa acaattgttg
     4921 gatcttctga tcaaagctct ccggttgcca ttgttcattc aattgttcac gaaaaacaag
     4981 gctacatgtt tttttatgaa aattatttaa ataccggtcg taataaccac cgccaaaacc
     5041 gattcgataa ccggctctat tgtagataat acctggtaca atgaggagat cgatcatctc
     5101 cggcaaatag agcttctctg attgtggctc aagaacacca aaactcgaac gtacaagaac
     5161 ggactgttca tccatttcat aaaacgccat ctgacgtttc ggcaaagttt taggtacaac
     5221 tattttcttt tgttcttttt ctgctcgttg gataaccggc tgtgtatcca gttcaaattc
     5281 attcgataaa gtcgtcccta tcacttttgc tgacttccag tactcgctgg cgaatagctg
     5341 tttatagatt cgctgttctt tttccttttt cttgccactt ttggccagat ttttcagctt
     5401 ttcgattcct ttccggcgta atcttacttt caattggtcc aaaaactcac ccttccttct
     5461 ctcataaaat ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa taaatgcata
     5521 aatagaataa aaagaagacc tagaaatgat tggccttcct tttttaatga atttcaactg
     5581 ttcagcgcgt gtttttgtct gataaaaagt gcatacagaa aatatggcgc accaaaaagg
     5641 gcaatcaaaa ttcctgtagg tacttcccca cttagtagaa tcactctagc aaccatatct
     5701 gcactaagca gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca gttattttct
     5761 ttacgtacga ctagcttcgc agcatgagga gctagtagtc ctacaaaaga taaactacct
     5821 gtcaccgcta cactaccgct tgccaaagca acagctaaca acaaaaggat tttttgactt
     5881 ttagccacgt taaggcctaa tccttgcgcg cgttcttgac caaatgatag caatacaatc
     5941 ctctgtgcat aaagtcctcc tagcggaatt aagataaccg cccatggaag caatgcccaa
     6001 acataaggcc agctcgcacc ccagattgat cccgctaacc atgcattcac aaatccataa
     6061 ttttccttag ataattggat cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg
     6121 gcaataccgg acaataagat tcgattcata cccaaaaagc catttcgatc ataggccgca
     6181 agatagacac agcaagcagc taaaacacca cctagaaagg ctacaactag caatagaaac
     6241 ggcagcgaat agtgcgaaaa gaaacctaaa taaagcatca ctaaaaatcc tgctccggca
     6301 ttaatgccca atatactaga atctgccaat tcatttcttg ttactccttg caagatataa
     6361 ccagaaacgc caagacaagc tcctgctaat aaactgatca gtaatcgagg aactcgaaat
     6421 tctctaacaa tcagcgccaa agaagatgat tctttcccta tgaacgtggc aaaaagctga
     6481 tccaggtaaa taaaaggcgt accaatcatt aaactaccta tagacaggat gacaagtaca
     6541 aaaagcaaga aatagaacgt ccaatatatt tttttcaccg ttgttccctc cttacttgta
     6601 tcagtaaaaa cggaacgcca atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg
     6661 gattgatcat cctcgctgta aaatccgcca acataaccaa tccaccgcca actaaaaaag
     6721 agacaggtag aacataacga tagtccgttc caaccattcc gcgaacaact ggcggaacca
     6781 acagcccaat aaatgaaaca ggtccgacta acgaaacggc aatggctgct aacactgcaa
     6841 ctagacagaa aacaatcaac cgcgttcgtt taggatgact tccaagagct aatgcatgtg
     6901 tttcactcaa atgaatcaaa gatattgctt ttcctaatga aaaagaaacc aaaacagcaa
     6961 gtatatataa aggaaacaat actgctaatt gtttccatgt gatattggca gctccgccta
     7021 caaaccaaaa agcaagatcc tgctgcagat taaagaattg ggtaagtgct tggctgatag
     7081 cagtaaaaaa agaactaata gctacgccag ctaaaatcag ctgtaaagga ttcatgccca
     7141 ttttcgaaaa ctgcaaaaaa ccaaatacga gcaaagttac agcaaaagaa cctgccaatg
     7201 acattagaaa cacagaaaaa tcagaagctt taggccaaaa agcaaaacta agggccattg
     7261 ccaacgaagc acccgcgttg attcctagta agcctgaatc tgctaaagga tttcttgtca
     7321 ctccttgcat caatgcaccg ctcaatgcaa aacaactccc tacaagaaat gctcccgcca
     7381 cacgaggcaa acgaatattt cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa
     7441 acaacgcctg ccaaaaatct gcttttccca cagtcgctgc gccatttgaa agagcaacaa
     7501 aaaaaataat caaaagaaca aaacagacca aaagcaggag tctatttttc ttcatagtcc
     7561 tcataattga tcctcctgct cttttggcaa caaatcatag gttagaaaaa ctggtttatt
     7621 tgttcttgga agcgtgacaa tcgttgcatc aatctcaaat atttttttca tacactcttt
     7681 tgtgatcact tcttcaggtg taccttctgt gatcaactta ccgtttttca ttgctaataa
     7741 ataatctgaa aaacgtgaag catgattaat gtcatgaatc gacatcaaga tcgttgtttg
     7801 tttcatttga ttgatttcag tcaataactg taggatctct aattggtgtg ccggatctaa
     7861 aaatgttgtc ggttcatcca agatcaagat atctgtttcc tgagcaagcg ccatagcgat
     7921 ccagacacgc tgggtttgtc caccagacag cgttgcgata tcccgcatac gaaatccagc
     7981 tagacctgta gcatccaacg cccactcgat tgcttcatga tcttgcgtgt ccatgccact
     8041 gaacagtttt tgataagggt atcgtccgta agatactact tcttctactg tcatacctgt
     8101 agggtgttca ctagtttgtg ctaataatgc aatttttttt gcaagttctt ttgaactgta
     8161 tgtagagata tcagagttat tgacaatgat ctttcctgat ttcacaggta gtatacgggt
     8221 aaaacttttc aataaagtag attttccact tccatttggc ccaatcaaac tagtgatttt
     8281 cccttcagga atcgtaacag acatgttttc aatgattttt ttgttttcgt agccggctgc
     8341 aatgtcagct gctcttaatc tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa
     8401 ttatgacgct gtttcaacat ttcgtcaagc gctactttca tatctatagc caaatgaagc
     8461 ggttgacagc gttcgaaata tcaggaataa taaccaatga gaaagattct caattatgtt
     8521 aggaggaaag aaatgaaatt aaaaacaaaa gtattcacat tatctagcat attgctgctg
     8581 gctggagtac tgagcgcttg ctccccctct tcagataaag aaacagctac aactgccaat
     8641 tcgacgactt catccaatag tactcataca gtcacagata cacttggaca taaagtagag
     8701 attccaaacg cgcccaaacg gatcatcggc agttacttag aagactatct gattgcacta
     8761 ggggaaaaac ctatcgccca gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa
     8821 gatgatttaa aggatgtccc aacgatttct tatgaccttc cttatgagaa agtgttaagc
     8881 tttgagcctg atttgctgtt gatttcttct tccgctactg tcgaaggcgg gaaatacgaa
     8941 caatatagca aaatcgcgcc aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa
     9001 gaacaactaa aagatgtcgg caaagctttg gcaaaagaaa aagaggcaga aaagatcatt
     9061 tccgattatc aaaaatccat caagaaaact cgagaagaac tagcagataa aatcgagaac
     9121 aagacagctg cagtgttgtg ggtgaccaac aactcagcct ttatggtcag caaaaatcgg
     9181 tcaagtggaa gaatcgtata cgacgatctg gaatttggtg ttcctgatct agtggaagag
     9241 gtctccaaaa aagccacttc tgactggtct gctgtttcct ctgaaaagct agctgaatta
     9301 gatgctgact acatcatcct tgtgaacagt gacaaagatg ctgccatgtt caatgaacca
     9361 agctggaaga atcttaaagc agtgacagac aatcatgtac tgcaattcgg acctgaatct
     9421 agttggctat ataacggacc tattgccagt caggaaatgg tcaatgatat caaagaaaaa
     9481 ctaaattgat tttaactaaa aaaagcatct tcacgattag tcgtgatatg ctttttttct
     9541 tattaacaaa ctagacgcat agattttatt cttcttttga agcaacccta ttttttactc
     9601 tttcccattt ttgaaaacga tgggcatata gattttcttg atccgttatc ccctcatact
     9661 cctctataag ggcgaagtct gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg
     9721 tgctatcgat caatgttcgc cagatccgac cagtgtaagg aagaagttct tgaaaaatcc
     9781 gactaccacc cgaaacatag atatcttctg agatcgtttg actatacgac aaaagttcat
     9841 cgatcgaatg cattacttcc gcattttctt tcgtgcgaaa atttggctgc gttgtcaaca
     9901 cgatgatatg gcgataagga agtgaaagat cccccattcc ttcataggtc tttcttccca
     9961 tcactaaaat tcgatccatt gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc
    10021 atggtaacaa accatttttg ccgatcagac catttttatc ttgtgcccac atggaaataa
    10081 acatattatc cgctccttat gattttagat gttttgagat aaaaaaagtc tcaattcttt
    10141 gaccagcaag gaattgagaa ctttaaaaac attattttgt ttcacggtgc aaagtaactt
    10201 tgcgttcgcg tgggcaatat ttttgttttt ccaaacgatc aggattgtta cgtttgtttt
    10261 tgcttgttag atagttacgt tctttacaag aagtacactc taaagtgatg tttacgcgca
    10321 tgtttttccc tcccattttt acaagaatcc tctaagatga ctctcagact tgattatctt
    10381 accatgattt tttctagatt gctagtgttt ttcacgattt tgttaagtaa aactacttga
    10441 cgataaatac aacggtttta cttctcaaac aaaatctttt tctttttgta taaataccct
    10501 tacacttgtt tatatagatt atgtttttta ataaaggggg atactaatat gccatggaac
    10561 atgaaggact ttcctgcatc aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc
    10621 gacatcgcaa atgcattatt agatgaaggg taccctgata accgtgcgat cccaattgcc
    10681 attgatcaag caaaaaaatg gtatgaagat gctagtgaat cagaaaaacg aactttcgaa
    10741 aaagaaaaaa atccaagcaa gacggatgaa cacgatacga atcctcgtgc tggaaaactg
    10801 cttgattcag atgtgatcgt agaatatgag gaagaacaat ggatcgtgaa atctaaaggg
    10861 gcaaaaaagg caagcaacca ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa
    10921 gtagcacaaa acaaagaatc aactttgttc atttataaaa aagatgggac aaaagaaaaa
    10981 gaaatcgatt attgaacata tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt
    11041 atgttcgaaa caatggtgtt cctctgccaa ctccgaacca taagtcataa tatctataaa
    11101 acgggaagag ctgttttttg aaattgtttc ttatcactca gtgttaaacg gtaggagaaa
    11161 acctcttttt tattgatatt tgattttcat caaacagtca catagtcgat tgcttctgct
    11221 tgcggaatcg tttcatctac aaagtagact ttataccatg tcaagaaact gaagattgtc
    11281 cagatttttc tttgctcttc tttacgtcct tcgtgatggt catctagcaa cttcatgatt
    11341 ttttcttgat caaaaaattc ttttgcaaaa tctgcttcga acaatgcttt gacttgttcg
    11401 tagccgtgtt cttctttcaa ccaagctttg attggcacag ggaatcctaa tttcacacga
    11461 ttcgaccatt tttccggaag atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg
    11521 ttttgattca ataaaaactt acttggtatt tgttgagcca atttcatgac ttcgatatcc
    11581 aataaaggta ctcggacttc caaagaactt gccatagaaa gtttatctgc ctttaacaag
    11641 atatcttttg gcatccattg atgaagatcg acatattgca tcttattgac ttcatctttg
    11701 attccttctg ttttctcgta atgaacagtc atgatttcat ccacagatgg tgctgtttga
    11761 tattttggtg ttaacacttc tgatgcttct gattcttcga acactcgagc atgaccgata
    11821 aaatattctt ttgcaggagc aagtgattca tataaatgga tccgtccatg gaaatttttc
    11881 atttttccga ttttacgtcc taaattatag cgtgttcctt ttggtaattt tttcaatcct
    11941 tgagcaatca cacggatgaa ttttgaattc gtatggaatc catacgtttg gtatcccgca
    12001 aacagttcat ccgccccttc tccagattgg actacccgca cactttgtga agcaagattt
    12061 gctaaaaagt acaaaggtac gcaagaagga tttgaatcag gttcatctaa gtgatactgg
    12121 atcaaaggga atgctttgaa cgacatgtct ccgtcgatca ctgctgctgt attatctaag
    12181 tccaatagtt cggttagttt tctggcttca atcgcttcat tataggtttt atcatcaaac
    12241 ccgattgaga aagaatgatc tggtttcaat acagaggtta cataactgga atcaaccccg
    12301 ctggataaga aagaaccaac ttctacatca ctgaccgtat gtgcttcaat agaagcttga
    12361 acggtttcgt cgattttttc gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa
    12421 tctgcatccc aatattgctg aatgtctatt tttccatctt tatacgtata ataatggcct
    12481 tcaggcaaac gatagacatc tttgaagaat gtttcgccgt ttaatggaga atattggaaa
    12541 gtcatataag gtttcaacgc ttctttgttc aattctttgt taaaatcagg atgaggcaga
    12601 aaacttttga tttctgaacc aaacataaat gttccgttca tttctgcata ataatatggt
    12661 ttgatcccaa agtggtctct tgcaccgaat aactcattct tttcattatc ccaaatagca
    12721 aaagcaaaca ttccgcggat cttttgcaat aattctgttc cccattcttc gtaaccatgc
    12781 aaaaggactt ctgtatctgc atgtgtttga aaaacgtggc cagctgcaat caattcttct
    12841 ctcaaaggct ggtaattata gatttcgcca ttaaaaataa tgatctttgt ccgatcttca
    12901 ttgtaaatag gctgagtacc gccctctaag tcaataatac ttaatcttct gaatcctaaa
    12961 gcaacatgtt tatcgatata ttctcctgaa ctattaggtc cacggtgaac gattcgatcc
    13021 atcattgtat taataattgt ttttttgttg tccttgtcgt taacaaaacc tacaataccg
    13081 cacatttaac tttcacatcc taatataaaa ttactgttta tttcattcat atgcatctta
    13141 tcatagaata aatagcggac gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa
    13201 agatgaaagt ttctgttttt acagaaaata aagtcccaat atctaattta ccatgttttc
    13261 ttttttgagg ctaccttatt ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa
    13321 aaaaaccagc aaaaaaagac acgacctaaa gatcgtgtct aattatgact gtatcagaaa
    13381 atatgatacc aaacggatct attcattaat taattgacat agtttatctg ttgctctttt
    13441 atgactggat aactgaccaa ttcatgacga tccaagtctt ctttattcat atttacagca
    13501 gtaatttgac tgtcttcata cgtacgataa taataaatcc ctttatcaac gttacagcaa
    13561 gaagaataaa tcgtatattc gtattttctt tctccagtat cgcatactcc tttttgttgc
    13621 tcgactgagc ctagtatatg aaaaaattga ctgatacttt cagattctga atcacctgat
    13681 gcagcattca ttttagtaaa tgtggctttt acaaatcgag aaacagaaga gagatctcca
    13741 ggtaagccca atcctcccat cccacgacta taaacattca agctgatctg gtttgaaaaa
    13801 tgattttctg gtgttccatt tgatagcgag cgataattgt tcagattaaa taattgataa
    13861 tcaaatggcg gattatttgt caacacaccg acaggattat catataaatg aagtccatct
    13921 ttcatacttt caattactat cgatttttct ttatcagcta ataaccagtg taaaggtgac
    13981 aagggtagtt tttcactaaa atcgatattg actagattga tagtagccaa taattcttta
    14041 gcttcgtcta tggttgagca ttgccctagt atccatggaa taaactcaaa tggtgcaacg
    14101 ttatcttttc cttcttgtaa ttctttataa tcagcattgc caggaaaatt cagtcctgcc
    14161 atactcaacc ctttttcatt tgtagcctca taatagagtg gatagttgga taccccagct
    14221 gctattccta tcattgcata atgtttatct aaatttttta cctttctaaa atcgaaacga
    14281 taatttcttg gagtaatagt gaccacttca ttataagaca tctcataatc aaaattcctt
    14341 ccaaaataat ggtctttcgt tgcataagtg attgcggtac acatcgtttc ccctcctaaa
    14401 acaaatcgtt tttacacttt tatcttacat ctagtatttt ttttatcaaa aaaaggccaa
    14461 agaagattga tttactagca tttataaaaa atagtactca gggggaatca aaaaatattg
    14521 taggttaaaa gtacaacaat aaggggctgt gacaaaagtc ttgtcacagc cccttattcc
    14581 gaacaaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa cgaaaaaata
    14641 ggaaaagcta tttttgtttg gccgttctta ttacttgatg caggacagct tcttcacaac
    14701 ctctaatctt tgatataagg aaggtacagg atttgaacct gcgcgccggt attagccggt
    14761 tcgccggatt tcgagtccgg tgcattacca ctctgccaac cttccgtaag tactattttt
    14821 aaactttttt cacgaaatgt caagcctatt tctaatagat aaataacaga aatcgctttt
    14881 tttagcaaaa aagtccattg acagtttgca gccaacggac ttaccgatca aattcatact
    14941 gatagaacac tatctgatta tttcaggatt atctaatgct aaaatcgagt caattgtccg
    15001 tttgatcagt gcaatgattt gttcttctgt tggatgcatg atgtttgatg acatcaatga
    15061 cgctaagcca gtcactgcta cccatgtacc agtatgcaga gcatcgacat attcatccga
    15121 taattgattg tatctttcat cttctttaac tatttcacgg aaataattgt atgaaaaatc
    15181 atgcattttc tttccgtccc catgttcatc aatgtacaat gccatgtaaa gactatgatg
    15241 cttttttgca aagtagatat agttcaaacc gagatcaatg attttatcac cagtacgttc
    15301 aatagggaaa acatcttctt ttagttgttc aaagatagaa tccagcaacg tattttttaa
    15361 atcttccatg tttttgaatt ccagataaat cggctgtgtc gaaatcccca tctttttagc
    15421 aatatttctc gcagtaaatc ctttaaaccc ttcctttgca acaacatcat aagcagcttt
    15481 tagaatttga tccttggtat agacttttct tctcattaaa actcccccac ttctttactg
    15541 cagtatatgt tg
//
LOCUS       DAZKNM010000056        15245 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000056 DAZKNM010000000
VERSION     DAZKNM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.056"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG8M9_001399"
     CDS             2..1021
                     /locus_tag="ACG8M9_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0378183.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG8M9_001400"
     CDS             1018..1785
                     /locus_tag="ACG8M9_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0378184.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8M9_001401"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8M9_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0378185.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG8M9_001402"
     CDS             3750..4070
                     /locus_tag="ACG8M9_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0378186.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG8M9_001403"
     CDS             4070..4420
                     /locus_tag="ACG8M9_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0378187.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8M9_001404"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8M9_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0378188.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8M9_001405"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8M9_001405"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0378189.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG8M9_001406"
     CDS             6414..7439
                     /locus_tag="ACG8M9_001406"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0378190.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG8M9_001407"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG8M9_001407"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0378191.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG8M9_001408"
     CDS             8517..8957
                     /locus_tag="ACG8M9_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0378192.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG8M9_001409"
     CDS             8960..9562
                     /locus_tag="ACG8M9_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0378193.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG8M9_001410"
     CDS             complement(9625..10728)
                     /locus_tag="ACG8M9_001410"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0378194.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8M9_001411"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8M9_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0378195.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG8M9_001412"
     CDS             12294..12689
                     /locus_tag="ACG8M9_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378196.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG8M9_001413"
     CDS             12689..12940
                     /locus_tag="ACG8M9_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378197.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG8M9_001414"
     CDS             12983..13378
                     /locus_tag="ACG8M9_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378198.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG8M9_001415"
     CDS             13375..14631
                     /locus_tag="ACG8M9_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0378199.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG8M9_001416"
     CDS             14643..14966
                     /locus_tag="ACG8M9_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378200.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG8M9_001417"
     CDS             14959..15225
                     /locus_tag="ACG8M9_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378201.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5238 a         2580 c         3223 g         4204 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaatt
//
LOCUS       DAZKNM010000057        15065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000057 DAZKNM010000000
VERSION     DAZKNM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.057"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..2629
                     /gene="adhE"
                     /locus_tag="ACG8M9_001418"
                     /gene_synonym="adhC"
     CDS             32..2629
                     /gene="adhE"
                     /locus_tag="ACG8M9_001418"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0378202.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2858..3964
                     /locus_tag="ACG8M9_001419"
     CDS             2858..3964
                     /locus_tag="ACG8M9_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0378203.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3981..5009
                     /locus_tag="ACG8M9_001420"
     CDS             3981..5009
                     /locus_tag="ACG8M9_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0378204.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5015..5494
                     /locus_tag="ACG8M9_001421"
     CDS             5015..5494
                     /locus_tag="ACG8M9_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0378205.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5510..6805
                     /locus_tag="ACG8M9_001422"
     CDS             5510..6805
                     /locus_tag="ACG8M9_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0378206.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6816..7640
                     /locus_tag="ACG8M9_001423"
     CDS             6816..7640
                     /locus_tag="ACG8M9_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0378207.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7805..8704
                     /locus_tag="ACG8M9_001424"
     CDS             7805..8704
                     /locus_tag="ACG8M9_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378208.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8830..9699)
                     /locus_tag="ACG8M9_001425"
     CDS             complement(8830..9699)
                     /locus_tag="ACG8M9_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0378209.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9852..11312
                     /gene="rhaB"
                     /locus_tag="ACG8M9_001426"
     CDS             9852..11312
                     /gene="rhaB"
                     /locus_tag="ACG8M9_001426"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0378210.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11309..12565
                     /gene="rhaA"
                     /locus_tag="ACG8M9_001427"
     CDS             11309..12565
                     /gene="rhaA"
                     /locus_tag="ACG8M9_001427"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0378211.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12579..13424
                     /gene="rhaD"
                     /locus_tag="ACG8M9_001428"
     CDS             12579..13424
                     /gene="rhaD"
                     /locus_tag="ACG8M9_001428"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0378212.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13426..13737
                     /gene="rhaM"
                     /locus_tag="ACG8M9_001429"
     CDS             13426..13737
                     /gene="rhaM"
                     /locus_tag="ACG8M9_001429"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0378213.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13819..14970
                     /gene="fucO"
                     /locus_tag="ACG8M9_001430"
     CDS             13819..14970
                     /gene="fucO"
                     /locus_tag="ACG8M9_001430"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0378214.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
BASE COUNT         4831 a         2593 c         3416 g         4225 t
ORIGIN      
        1 caaataacca tgacccatta ggaggataat gatggctaaa gtaatagaaa aagaaaaaac
       61 gaagacaatt gacgtacaag caatgattga tgatttggca gaaaaagcaa atgcagcatt
      121 gaaagaaatg gaaagctttg atcaagaaaa agtagaccat atcgtgcatg aaatggcaat
      181 ggcagctttg gatcaacata tgccattagc gaagatggct gtagaggaaa caggccgcgg
      241 tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc gaatatattt ggcataatat
      301 caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc caaaaagggt tgattgaaat
      361 tgctgaacca gtcggtgtgg tttgcggagt aactccaaca acgaatccga cttccacaac
      421 gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca atcgtttttg ctttccatcc
      481 aagtgcccaa aaatcttctg cagaagcagc acgagtggta cgtgatgctg ctatcgctgc
      541 cggagctcct gaaaattgta tccaatggat cgaacatcct tcgatcgaag cgacttctat
      601 gctaatgaac catccaggta tcgcgatcgt cttagcgaca ggtggagctg gaatggtcaa
      661 gtctgcttac tctacaggaa aaccagcttt aggtgtaggt ccaggtaacg taccagctta
      721 tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat ttgatcgttt cgaaaacatt
      781 tgataatgga atgatttgtg cttctgaaca agctgtgatc gtggataaag aaatctatgc
      841 agcagtaaaa gcagaattcc aagcacacca agtttatatc gtaaaaccag atgaattgca
      901 aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg gtcaatccat caatcgttgg
      961 acattctgcg atggaaatcg caaaattggc aggaatcagt gtaccaaaag gaacgaaaat
     1021 gctgattgct gaattggaag gcgtaggccc agattatccg ctatcaagag aaaaactttc
     1081 tccagttctt gccatgatca aagctaacaa cacggaccat gcgtttgatt tgtgtgaagg
     1141 tatgttgaat cttggaggat taggacatac agcagttatc cattcagaaa acgaagagtt
     1201 gcatgtcaaa ttcggtcttc gaatgaaagc ttgtcgtatt ttagtgaata caccatctgc
     1261 agaaggcggc atcggtgata tctataacga aatgattccg tcattgactt tgggatgcgg
     1321 ttcttatggt aagaactctg tttctcgtaa cgtctctgct gtaaacttaa tcaatgtcaa
     1381 aactgtagcg aaacggagaa ataacatgca atggttcaaa ttaccgccaa aaatcttctt
     1441 tgaaaagaac tctctattgt acttagagaa aatggaaaac gttgaacgtg tcatgatcgt
     1501 ttgtgaccct ggtatggtcc aattcggtta ctgcgataca gttcgtgaag tattggcacg
     1561 acgtaaaaat gatgtgaaga tcgaagtctt ctcagatgtt gaaccgaacc catcgacaaa
     1621 taccgtatac gcaggaacga aactgatggc agacttcaag ccagatacag taatcgctct
     1681 tggaggcggt tctgcaatgg acgcagctaa gggaatgtgg atgttctatg aacacccaga
     1741 cacatctttc tttggtgcaa aacaaaaatt cttggatatc cgtaaacgga catataagat
     1801 cgacaaacca gaaaaaacac aattcgtatg tatcccgaca acatcaggga ctggttctga
     1861 agtaacgcca tttgcggtca tcactgacag tgaaacacat gtgaaatatc cgttagcaga
     1921 ttatgcattg acgccagatg tcgcaatcgt cgatcctcaa ttcgtcatgt cagttccagc
     1981 atctgtcaca gcagataccg ggatggatgt gttaacacat gcaatcgaat cctatgtttc
     2041 agtcatggct tcagattata cacgtggatt gagcctacaa gcaatcaagt tagtctttga
     2101 ctatttagaa aaatcagtaa aaacacctga tatggaatca cgtgaaaaaa tgcacaacgc
     2161 ttctacaatg gctggtatgg cgtttgctaa cgcattccta ggtatttgtc actcagtcgc
     2221 acataaaatc ggcggggaat acgggatccc tcatggacgg acaaatgcga ttcttttacc
     2281 gcatattatc cgctataatg caaaagatcc acaaaaacat gcgatgttcc ctaaatatga
     2341 ctatttccgt gcagatacag attacgcaga tatcgcaaaa ttcttaggtc tgaaaggcga
     2401 aacaacagag gaattagtcg aagctttagc aacggctgtt tatgaattag gaaaatctgt
     2461 cggcatcaac atgagtctaa aagctcaagg agtcacacaa gaaacactag atacaaccgt
     2521 agaccgtatg gctgaattag cttacgaaga ccaatgtacg acagctaatc cgaaagaacc
     2581 attgatctct gaattgaaac agatcatcat tgatgcttac aacggataag caaaagaaag
     2641 cgtagaaaaa agataagaaa atagatgaaa aagaacttgg aaaacgttgt ttctccaagt
     2701 tcttttttta atttccaagt aactgaaagt tacttttgtt atttaacatt taggtcaatt
     2761 ttagacagtt tttgttcaaa aagcgatatt tatagtggac ggtattactg ctatattaag
     2821 gatataaaat gaaagcgttt aaaaaggtgg cgtaaagatg attattaaag aagagatttt
     2881 tgtacctaaa gaattagtga aagaaaaaat tgacctgctc gttgaaaacc tgacaacgat
     2941 caaagacgat aatggtgaat ttttgttgga ctttgatgga ctgaaagtag atgacaaaag
     3001 ctggacagtg tggaattggc cacaaggtgt cggtctatat ggtatttata aaaattacca
     3061 gatgacaaaa aatcctcgcg cttatcaagt ggtcaatgaa tggtttgagg atcgtatgga
     3121 agaaggcgct ccgcccaaaa acgtcaatac aatggcaccg ctattgacta tggcttactt
     3181 gtatgaagac acgaaagatt cacgctattt gccttattta gaacaatggg cagaatgggt
     3241 catgaatgac atgcctcgga caaatgaaga cgggttacag catgccacat atggaccgga
     3301 aaataagaat cagttgtggg acgatacatt gatgatgaca gtgcttcctt tggcaaaaat
     3361 cgggaaactg ttgaatcgcc cagaatattt agaagaagct cgttaccagt ttatgatcca
     3421 tatcaaatac ttgatggata aacgaacagg attgtggtat cacggctgga cgtttgaagg
     3481 aaatcataac tatgccgaag cattttgggc tcgtggaaac tgctggttga cgattgctat
     3541 tccagagatc attgaaatct tagaattatc gaaagaagat gcattgcgca aattattgat
     3601 cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat cagtctgaaa gcggtttatg
     3661 gcatacgttg ttggatgatc cttcttctta cgtagaatct tctgcaactg caggatttgc
     3721 ttacggtatt ttgaaagcgg tccataaacg ttatttacca caagaatata aagaagtcgc
     3781 ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa aaaggagaag ttcaaaacgt
     3841 ttcaatcggg acaggaatgg gggacagttt agatttttat cggaatatcg gaatcacagc
     3901 aatgccttat ggacagtcgt taacggtgct ttgtttgaca gaattgctcg tctcttattg
     3961 ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct gtttgcagca agttcgctta
     4021 gcctaggtct gctaggtcta agcggctgcg gaaataattc tagtgtggca actgtcgcag
     4081 atgcagatgt aaatgagata gttaccttgc gctttgctta tgcaagcaac agccagccag
     4141 taattgatgc aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac
     4201 agatccagta tttcccagat ggacaactgg gtggagaacg tgaactgatc gaattgacac
     4261 agtcaggagc agtagatttc acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag
     4321 attattcgat tttttcagtt ccttatctat tcaatgatga agaacatttc tttaaggtga
     4381 tggataatga agaaataatg gagccaatct accaatcgac caaaaattta ggatttaccg
     4441 gattgaccta ttatgattct ggtcagcgca gcttttatat ggtagacggt ccgatccata
     4501 cgccggaaga tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa
     4561 tggtagagct tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc
     4621 ttcaatccaa tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat acagctggac
     4681 atggtggtgt ggctaaatac tattcctatg atgagcatac acgtgtgcca gatatcatca
     4741 tcatgaatga tgccatcaaa gaacagctaa ctgatgagca ggaaaaagcc attgaagaag
     4801 cagcgaaaga atcaacggtt tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa
     4861 aagctttagc acaagaagaa catggagtcc aattcaatga tgtagataat acaccgtttc
     4921 gagaggcagt tgccccactt catgatatat tcaaaaacga tcctacctac aaagaccttt
     4981 atcaaaagat ccaagagcaa ggagcgtagc tgtgatgaaa atgattttaa acaaggtatt
     5041 agagatcctg ggagctgttt tgcttgttgg gatggtcgct gttgttttat ggcaagtatt
     5101 ttccagaacg gttttaggaa atccaaatac ggtcacggaa gagcttgttc gattcggtct
     5161 tgtctggttt gccatgttgt cttccgcgta tgttgtggga caaaaagcac atttagccgt
     5221 aacggtttta agtgaaaaat taactggcaa aaaaagtaaa attttagaag ttgttgttca
     5281 aagtttattt ctggtatttg ctgcgatgat catggtgtat ggcggatgga atgctgtcac
     5341 attgacaatg gggcagatct cgccttcttt aagtattcct atgggatatg tttatctgtc
     5401 agtacctgta tcgggggtac tcatcattat ctatagcctg atcaatctaa tcgatacatt
     5461 aagcaacaag ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa tggcaataga
     5521 agccggatta gtgttatttt ttgtatttgt tgtgttgctt gtattaggga tgccgattgc
     5581 tataagtatt gcattctcat cgatgatgac tttattgtta gttataccgt ttgatgtttc
     5641 tgcttttagt tcagctcaaa aaatggtggg aagtatcaat agtttttcgc tgatcgctat
     5701 tccgtttttc gttctctcgg gcattatcat gaataatgga gggatagcta agaaactagt
     5761 ggattttgcg atgttgttcg tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt
     5821 agggaatgct ttatttggtt ccatctctag ctcggccatt gccgcttcta ccgcgattgg
     5881 cggagtgttg atccctcagc aagtggaaga aggatatgac cggaagtttg cgacagcggt
     5941 caatatcgcg tcagctccga caggtatggt cattcctcca agtactgctt ttatcatgta
     6001 ttcacttgtt gctggtggtg cttcgatctc tgctttgttt atgggaggtt atctcgtagg
     6061 cgctttgtgg gcgctaggaa tcatgctagt agcattcatc gttgctaaaa agaataaata
     6121 ccctgttgtc gaaaaaggac agatggctca tgctggaaaa ataataaaag aggcgattcc
     6181 tagccttcta ttgattgtca tcattatcgg tggtatcttg acggggatat ttacagcagt
     6241 agaagcttca gcgatagctg tcatttactc cctgttgatt gctatgtttt actataaaac
     6301 ggtaaaacta aaagatatac ctaatatgtt gtttcaatca gtagcaatgt cagggacgat
     6361 catgttctta ttggctactt cttccatgat gtctttcgcc atggcgttta ccggtattcc
     6421 tcaggcaatt agttcgttga ttatgggcgt aacggataat aaatttatta ttctgttatt
     6481 agtcaatctt gtgttattac tggtgggtat gtttatggat gtggcgcctg ctatcttgat
     6541 ttttacacca atatttttac caatcgtcac aagtgtaggg gttgatcctg tacattacgg
     6601 gttgttttca atcatgaatc tgtgtgtagg ctccatcacg ccgccagtag gtacaggact
     6661 atatgtcggt gcaagtgtag gaggcgtaaa agcagaggca atgctgaaac cgcttcttcc
     6721 tttttatggg gttatcttag tcgtcttgtt tttgattact tacttcccgc aaatcgtcat
     6781 gtggctaccc aatatgctga gttaggagaa tagttatgtt gaacattaca attagaggac
     6841 atgatctttc cgaagtccaa acgatagaag atcttgcaga aaaaacaaaa gaacaaggga
     6901 ttcatacact gcagctcgcc ttaggtcttt cgtttcctga aataccttct ggcgcaaatg
     6961 agatcaattc tgggatggga aattatgtca aacgagtttt agaaaagcaa gaagtgaccg
     7021 taggaatttt aagttgctat atcaatatga ttcatccaga tttaacgata cgacaagagt
     7081 tgctgacaaa gttcgagcag tatcttcgct atgcttcttc ctttggtgcg tcgatggttg
     7141 cttcggaaac tggatgtgtt ttgccggaaa tccaatatac ggaagaaaat ttcacagatg
     7201 aagcatttgc tgaagctgtg tccgttatcc gtcgcttggt aaaagctgga gaaaaatacc
     7261 aaatgatggt ggggatagaa ccaggtttga atcatccggt gtattcttta gcaagggtag
     7321 agcagctgat ccaagcagta gattcggact atctaggaat cattttagat ccgaccaatt
     7381 tgatcacaag tagtgattat caagaacaag tccaattggt agaagaagct tttgaacgtt
     7441 ttggtgaaaa gatatgtgcg gttcatttga aagattttcg tgtcgaacag gaaaaaatcg
     7501 tgccggtaaa tttgggagac ggtgtgatcg agtacacaaa aatcaaagaa atcatcatga
     7561 agaatcgtcc gtacctgtat gtggttcttg aagaaacaaa agatgatggg attcgttatg
     7621 gaagaagtct tcttgaataa tgtaaagaag acgttggtta actaatattt agaatggagg
     7681 gggcggggct agaagatgcc acgtctcttc ttttttataa gagtatataa aagcagctct
     7741 ttttgattac aaaaaacgtg gtataataaa attgtgcata agtgaacaaa aggaggtgcg
     7801 gcacatgctc caaaagaaaa tcagcggtga acaagtcaat acgatggttt ctgatcagta
     7861 tgaaatctat catgtcaaag atgtcgtcag ccaaaataaa acgatctatc atcatcatga
     7921 tttctatgag atccatgcta cattaaaggg agaagcgact ttttatttga acggacgaca
     7981 atttacgatc aaagcaggaa gtgtgctgct catccattcc aatgatttgc atcgtatcgt
     8041 ccgccaatct tcagatgtat tcgagcgtgt ctatatcttt gtcacccctt cttttctaga
     8101 aagtcgttcc acgccatggt ctaatctatc ggcttgtttt caaccaatgg gagagaagag
     8161 gagtcgtgta ttgcaaatag aacaggaagt attgaagcaa aaattatcat ttatcgacca
     8221 tcctcctgat aaaaaaattt acggtgaaga cataaaatat gaacaagctc tggtagaata
     8281 cttgattttt ctcaatcaag ttgtacaaaa agaagagagt gcgcaagaac agaatgctgt
     8341 agttcctaat gagcgtgtgg aacaaatgtt acaatatatc tcaaaaaatt tatctcatcc
     8401 gctttcttta gaggaaatgg aaaagcagtt ttttgtcagc aaataccata tcacacgaga
     8461 atttaaaaag tacactggat ttacgtttca tcagtatgta atgaagaaaa agctgatcta
     8521 tgccaagcac ttgttgcgag aataccgcag ctctagtgct gtatatagta aaagtggttt
     8581 ttcttcttat ccgcattttc tgaaagcatt caagagagag ttcggcataa cgcctaaaga
     8641 atttttgaaa cgtgatacac agaaacagtt ggttcattat gaacattttg aagatgaaca
     8701 atgagaatga tcactaaaaa ataaccgcag tagctgctgt tgtttacata gaagcagaaa
     8761 ctgcggttat tttattttta tctatatatt tcaaaatatt ttgaaaaaat gacgtacata
     8821 ttatatgaat cagctgaatt tacgaaagtc tctcggcgat agtcctgttt ttgctttgaa
     8881 caggcggcta aagtaagcaa aattctcaat ccctatttct tcggaaatca ttcgaaccgt
     8941 aaattctgta taccgaagaa ggtctttagc gtaagaaagt tttttgctga tcaaataatc
     9001 gattggtgga gaaccgataa atttggaaaa atctttgctc agctgatatt tattcaataa
     9061 gaattgttgt tctaattgat caagtgtaat cgtttctttg aaatgttcgt ctaagtattg
     9121 cttcagtttg taaacgtagg cgggaatatc ctcttcttcg aaatctagct gatacttttg
     9181 caaaatcagt tcattcagca actcgtggat agctacagag gactggaaat cggtacgggc
     9241 attcgtgcct tgttgcatgt ggatcaattc tgtgatcaaa tggtggatct tgttttctga
     9301 tggatctcct gttgaatgga aggtgttatg gccgtctttt gtgaactcct gataaaatga
     9361 ttggacgttc tgaccattca aatggatcca atccatttcc catggttctt cgctgatcgt
     9421 ttgataacgc tgatattctt ggcagtcaat aaaaaataag tctccagctt ttagctgttg
     9481 ctgattatca ttatatgtaa gcgaaccttt gccagacaaa gtaaacttta tcaagtaaga
     9541 aggtaaatgt gctcgttcag tatagtaagg gattggtgct ttgaagtaac cgatttcttg
     9601 gatataaaaa aataaattcc tgcttctttg tgtaggcgtc aagatcactc tttttgagtc
     9661 ttccgaccat gagtggttgg ctttttccca gtaatccatg taaacactcc caacttgtca
     9721 agataatgca attatatacc aaaatagtgt gaagcaaaat ataagattct gtattatgat
     9781 agcattgtca ataaatgata acgctttatt aataaagtgc taatcaacaa taaagtaaaa
     9841 gaggtggaga aatgaatcca acatttgttg ccatcgatat cggtgcatca agcggacgac
     9901 taatgaaaag tacgagggat gcaaaaggtt ctttgatctt ggaagaagta caccggttca
     9961 aaaacggctt tcggaacaaa gacggtcacg atcgctggaa gatcgattat cttatcaaag
    10021 agattttgat tggatttgaa aaattaaagc agacagggat tgatcgcctt tttgcgggga
    10081 tcgatacatg ggcagtcgat tactgtctga ttgatgaaca aggggatcgg ataaatgatc
    10141 cgattgctta tcgggacaag cgtaccaatg gagcagtaga acagttttct gaaatctatt
    10201 ctctagataa gttgtatgag cagacaggga ttcagatcca gcctttcaat accttgttcc
    10261 aattgtttgc tgaagaaaaa gaaaatttgg caaaagcaca taaattgtta ctgatacctg
    10321 attatttagg gtatgtcttt actggtaaca tggtgacgga gaaaactaat gcatctacta
    10381 tgcagctgct gaatgcaggg acaaaagaat gggaaataga acttcttgaa aaaatcggtg
    10441 tctcgaagga attatttcca ccattgacag agccgggaac gatactagga gatctgcaaa
    10501 aagaacaatt ttcaaaatat gatttgccag atgtgacctt tatcacagta gcaagtcatg
    10561 atacggcgtc agctgtatta ggaacgccag gtcaaggaga acgttggggt tatatcagca
    10621 gtgggacatg gtcgctttta gggattgaaa caaagatcgc caatgtttct cgcaatgctt
    10681 tttctgaaaa ttatacgaat gaatggggtg cccataacac ggtgcgtttt ttgaaaaaca
    10741 tcatggggat gtggttgatc caggaagttg cccgaatgca agagtacgag tacagttatg
    10801 cggaattggc agagcttgct gcaaaagaaa aaccgctgca gcaagtgatg gaaatcaatg
    10861 accctcgatt tttgaatccc ccaaatatga tagaagagat tcagcaatac tgttttgaaa
    10921 cagagcagcc agtccctgtc tcatcgggag ctatcgctcg ttgtgtgtat gataatcttg
    10981 ctctttgtta cgcacaagag ctgatcaaag tagaacactt aactggaacg aaattggaaa
    11041 agctgcacat cgtagggggc ggatcgaata atcgtctact gaatcaattg acagcagatt
    11101 atgccggaat cattgtagaa gcaggaccta gtgaagccac agcaatcggg aatgttgcca
    11161 tgcaaatgct cagtacaggt cagttttcga atatagaaca agcaagaaat gtaatcaaac
    11221 agtcgtttcc atgtgaaact ttttacccag atgaaaataa gcaaaacacg atggaatggt
    11281 acgtgaataa aatcaaagga gtaatcgtat gaacacgatt gaaagaaatt acgaacaagc
    11341 aaaagaaaaa tatgcaacta tcggtgtaga tacagatgct gtactagaaa agatacagga
    11401 catcaaaatt tctatgcatt gctggcaagg agatgatgtc aaaggtttct tgacaccgga
    11461 tggcgaattg accggtggaa tcatggcgac agggaatttt ccaggagccg cacgaacacc
    11521 tgaagagctt agacaagatc tggagaaggc ttattcgttg ataccgggca aacacaaatt
    11581 gaaccttcat gctatctatt tggatacaga agaatctgtc gatctaaatg aaattgagcc
    11641 gaaacatttt gaaaaatggg tagagtgggc aaaaaaagaa gaaatcggtc ttgacttcaa
    11701 tccgactttc ttttcgcatc caatgatgaa agacggcttt acattggctc atccagataa
    11761 agaagttcgt gaatactgga tcgaacacgg gaaaagaagc cgcaaaatcg cggagtactt
    11821 tgggaaagaa actgggcaaa caagtatcaa taacttttgg gtacctgatg ggatgaaaga
    11881 taatccgatt gatcgtttta ccccacggga acgtttaatg gaatcactcg atgaaatttt
    11941 tacagaagag ttagatgaac ggtatgtaca agatgcagta gaaagcaaac tatttggttt
    12001 aggcgctgaa gcttatacag tcggttccca tgaattttat atgggttacg ggttgactcg
    12061 tgacaaactt gtttgtctag atgctggaca ttttcatcca acagaagtga tttccaataa
    12121 attatcttca ttagctttat tcagcaaagg aatcatgttg catgtttccc gtccagtgcg
    12181 ttgggattca gaccatgtgg ttttgatgga tgatgaattg caggaaatcg ctaaagaatt
    12241 agtccgtaac gaattgttgg aaaaaacaaa tatcggtctg gatttctttg atgcaacgat
    12301 caaccgtatc gctgcttggg tcatcggtac gagaaataca caaaaagcac tgctgaaagc
    12361 tatgctggaa ccagttgagc gattgaaaga aatggagctg gcttttgact ttacttcaag
    12421 aatggcttat acggaagaat tgaaagattt cccgtatgcc gatgtatgga attatttctg
    12481 ttacaaaaat caagttccag ttggtttgga ttggctggaa gaagtccagg aatatcaaaa
    12541 agacgtgttg gaattaagaa aataggagtg tggctatatt gaaaaaaata gatattttac
    12601 aagcgcctta tgtgaaagaa atgatcgaaa cgacgtcgaa tctttatcgc ttgggatggg
    12661 atgaaagaaa tggcgggaat atttcttatt tactaaaaga agaagagatc acaccgttct
    12721 tggatcctga aaaagtgatc cgccgcatct cgatgatttt tgatgccagc cgtttagcag
    12781 gatgctattt tattgtaact ggttcaggaa gatattttaa aaatgtagcg agcgaacctg
    12841 ccgagaatct tggaatcgtt cgagttgctg aagatggcaa aacactggaa cttttgtggg
    12901 ggctggaaaa cgaatgcctt cctacaagcg agttgccaag tcatttcatg agtcatatcg
    12961 cccgtttaga agtagaccca gataatcgga tcgttatgca ttgccatgct agtcatctat
    13021 tagcgatgag ttttacgcat gaattagacg aacgttcctt tagtcgtacg ctatggcaaa
    13081 tgtgtacgga atgcttagtt gtttttccag aaggaatttc catcattccg tggatggttc
    13141 cgggaacaaa tgagattggg gaagcaacag ctgaaaaaat gaaagaaact cgattggttt
    13201 tatggccaca acatggaatc tacgggtcag gaaaagacat ggatgaagtc tttggtttga
    13261 tcgagacagc agaaaaggca gcagaagtct atacgtacgt caaagcgcaa ggaccaatct
    13321 tgcaaacaat cacggacgaa aacctgtggc atttagcaga tgcgtttggt gtcactccaa
    13381 aagcaggtta tttggaggaa gttcatacga aagcaggtgt ctgacatggt gaaaaaagct
    13441 ttttgcatga aagtttaccc agaaaaacaa gaagaataca aaaaaagaca tgatgaactt
    13501 tggccagaaa tgcgtacagc acttcgtgaa cacggggtca tcgactattc gatctatctt
    13561 gatccgagta caagtttgtt atttggttac ttagtgatcg aagatgaaaa gttatgggaa
    13621 aaaatggcaa atacatctat caatcaaaaa tggtggaagt acatggaaga tatcatggag
    13681 acaaatccag atgcctctcc agtcagttgg gaattgatgc cagtctttga actgtagcaa
    13741 caagcaactg ggcaaccggt tgcttggggt tctttcgttt ttattgtgaa aaataataca
    13801 aattaggagg aatagaaaat gagtaatcga atgattttaa atgaaacatc gtattttgga
    13861 aaaggagcga ttgagtcgat cgtccctgaa tttcagcagc gaggattcgg caaagcagct
    13921 gtcattactg acaaaggatt gatcgagcac ggtatagcaa caaaagtaac agaacttcta
    13981 gataaagcag caatcgctta tgctttatac gacgggatcg tacctaatcc aacgatccaa
    14041 aatgtaaaag atggagtggc ttttgttaaa gaggcagagg cagactgttt gatcgcaatc
    14101 ggtggaggtt cgccaatcga tacggcaaaa gccattggga ttattttgac gaatccagag
    14161 ttttcagacg tcattagttt agaaggtgtt gctgatacga aaaatccatg tttacctata
    14221 ttagctatac caacgacttc tggaacagca gcagaagtaa cgatcaacta cgtcatcaca
    14281 gatgaagtaa atcaccgaaa atttgtttgt gttgatcctc atgatatccc aatcgtcgcg
    14341 ttcatcgaca gcgatatgat gatgggaatg ccgaaaaaat tggctgcatc aacaggtatg
    14401 gatgctatga cgcatgctat tgaagggttt atcacaaaag gcgcttggga aatgactgat
    14461 atgctgcatt taaaagcaat cgaaatcatc ggccattctt tggaagcttc tgtagatggc
    14521 gatcaaaatg ggcgagaaaa aatggcatta ggacagtata ttgcgggaat gggcttctca
    14581 aatgtcggtt taggactggt acacgggatg gctcacccgt taagtgcatg gtacaacatt
    14641 cctcatggcg tagcgtgtgc agcacttttg ccaactgtca tgaaatataa caaagaatat
    14701 actggagaaa agtaccgtga gatcgctctt gttctaggga ttaaaggagc agctgaaatg
    14761 tctttagaag atgtaagaga agcggcttgt ggtgagatcg atcggttgag caaagcggtt
    14821 ggaatcccag aaaccatttc tgaattaggt gtaaaagaag cagatattcc agcaattgca
    14881 gaagatgctt tgcgagatgt atgcacacca ggaaatccaa gagaaacgac agtagaagaa
    14941 atcattgctt tatatcaaag tttgatgtaa aaacgaagcg gatcttcaac attaaattgc
    15001 ctgtaaagag aaatggtagt ttatatctgg atatctcttt taaattgatg gtatttccgc
    15061 tataa
//
LOCUS       DAZKNM010000058        14837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000058 DAZKNM010000000
VERSION     DAZKNM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.058"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..496
                     /locus_tag="ACG8M9_001431"
     CDS             62..496
                     /locus_tag="ACG8M9_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0378215.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            662..2707
                     /gene="parE"
                     /locus_tag="ACG8M9_001432"
     CDS             662..2707
                     /gene="parE"
                     /locus_tag="ACG8M9_001432"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0378216.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            2719..5169
                     /gene="parC"
                     /locus_tag="ACG8M9_001433"
     CDS             2719..5169
                     /gene="parC"
                     /locus_tag="ACG8M9_001433"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0378217.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            5446..7677
                     /gene="pflB"
                     /locus_tag="ACG8M9_001434"
     CDS             5446..7677
                     /gene="pflB"
                     /locus_tag="ACG8M9_001434"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0378218.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            7921..8682
                     /gene="pflA"
                     /locus_tag="ACG8M9_001435"
     CDS             7921..8682
                     /gene="pflA"
                     /locus_tag="ACG8M9_001435"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0378219.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            8767..9693
                     /locus_tag="ACG8M9_001436"
     CDS             8767..9693
                     /locus_tag="ACG8M9_001436"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0378220.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            9875..10768
                     /locus_tag="ACG8M9_001437"
     CDS             9875..10768
                     /locus_tag="ACG8M9_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0378221.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            10902..11027
                     /locus_tag="ACG8M9_001438"
     CDS             10902..11027
                     /locus_tag="ACG8M9_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0378222.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11161..11793)
                     /locus_tag="ACG8M9_001439"
     CDS             complement(11161..11793)
                     /locus_tag="ACG8M9_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378223.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(11941..12483)
                     /locus_tag="ACG8M9_001440"
     CDS             complement(11941..12483)
                     /locus_tag="ACG8M9_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0378224.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            12618..13007
                     /locus_tag="ACG8M9_001441"
     CDS             12618..13007
                     /locus_tag="ACG8M9_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378225.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13011..13775
                     /locus_tag="ACG8M9_001442"
     CDS             13011..13775
                     /locus_tag="ACG8M9_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0378226.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            13904..>14837
                     /locus_tag="ACG8M9_001443"
     CDS             13904..>14837
                     /locus_tag="ACG8M9_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0378227.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIR"
BASE COUNT         5218 a         2481 c         3123 g         4015 t
ORIGIN      
        1 gaaaaaatga aagatacaag tataatagat ggaataaaaa gaaaagcaag gggtggcaga
       61 aatgtctgta aaaaatccaa cacaagaaac gattttcaac tatttggaag aagcaaaaaa
      121 tattgccgtc gtaggcttga gcaataaacc agaacgaaca agttacatgg ttgcacaggt
      181 attgcaagac aatggatata aaatcatacc agtaaatcca ttattggctg gtgaagaaat
      241 tctaggcgag aaagtctatg cgaatttgac agaaatcgat gatccaatcg atatcgtaga
      301 tgtatttaga cggagtgaat ttttacctga aatagcaggt gattttttga aaactgatgc
      361 taaagtgttc tgggcacaat tagggattga aaacaaagaa gcagctgaga tgctaagaaa
      421 agcaggacgt acagatatcg taatggatcg ttgtattaag attgaacttg gtaagttgaa
      481 agaaaaccaa aaataaaaat gaccacaaag aagagatgat cgatagaggg gaatgtcgaa
      541 aaagcggtta ggagataatt aaaattgatt tctcgtctac cctctttttt ttcacgacct
      601 tttctagtat gataatcgtg gtgcggtttt gccgcaaagt attattgaag gagcgtttgc
      661 cttggcaaaa aaagtaaaca acgaatacaa tgactcgtcc atccaagtat tggaaggatt
      721 agaggcagtt agaaaaagac cagggatgta cattggatcc actgacagcc gaggcttgca
      781 tcatttagtc tatgaaatcg tcgataatgc agttgacgaa gcattatcag gttatggcag
      841 tgagattgat gtgaccatcc atgaagacaa tagtataact gttgcagata gcggacgtgg
      901 gatgcctgta ggaatgcatg cttccggaat accaacagtt gaagtgattt ttactgtgct
      961 gcatgctggc ggaaagtttg gtcaaggagg gtacaaaact tctggtggac tgcacggtgt
     1021 aggagccagt gtggtaaatg ccttatcgaa atggctaatt gtaacgatcg ttagagatgg
     1081 cgtagagtat cagcagaaat ttaaaaacgg cggtaaacct gacggtactt tgaaaaagat
     1141 aggaaaaaca aaaaaagcaa acgggacgac tgtccatttt ttaccagatg atacaatatt
     1201 cagtacaacg aaattttcat atgaaatttt agccgaacgg ttaagagaat ctgcctttct
     1261 tctaaaaggc gttaaaatct ctctttctga tcttcgtgga gaagaaccag tcaaggaaat
     1321 ttttcattac gaagaaggaa tcaaagaatt tgttgattat ctgaatgaag aaaaagatac
     1381 attgacacca gttgtttatt tttcaggaga aaaagaaggt atcgaagtag aggtcgctta
     1441 tcaatacaat gacggttatt cagaaaatgt tttatctttt gtcaataacg ttcggacaaa
     1501 agatggggga acccatgaag ctggtatgaa agcggcgatg acgaaatctt ataatgaata
     1561 tgcgagaaaa gttggtttgc tgaaagaacg ggacaagaat ctggaaggta gtgatttcag
     1621 agaagggtta gctgctgtac tttctatccg tgtacctgaa aatcttctgc aatttgaagg
     1681 gcaaacaaaa gaaaaacttg gtacgccagt tgctcgtacc gtagtagata acgtcatcag
     1741 tgaacaaatg ggcttttatt tgcaggaaaa tagcgaaatg agccagatgt tggtccgtaa
     1801 agcaatcaaa gccagagaag cgagagaagc agcacgtaaa gccagagaag aaagccgaaa
     1861 tgggaagaag cgtaaaaaag gcgaatctct tctttcagga aaattgacac ccgcacaatc
     1921 ccgaaaccct aagaaaaatg aattgtattt agtcgaaggg gattctgctg gcggatctgc
     1981 taaacaagga agagacagaa agttccaagc aattctacct ttacgaggaa aagtcatcaa
     2041 taccgaaaaa gctaaaatgc aagatatctt gaaaaatgaa gaaatcaata cgatgatcta
     2101 tacgattggt gcaggagtcg gtcctgagtt ttctatcgaa gactgtaatt acgacaaagt
     2161 aatcatcatg accgatgcgg atacagacgg cgcccatatt caagtattac tgcttacttt
     2221 cttttatcgt tatatgaaac cgctgattga agctgggaaa gtatatatcg cgttaccgcc
     2281 tttatataaa gtatccaaag gacaaggaaa gaagcaagtc attgaatatg cgtggacaga
     2341 tgacgaattg gcagcaatga ttaaaaaagt cggcaaagga tatatgctgc agcgatataa
     2401 aggacttgga gagatgaatg cggaacaatt gtgggaaaca acgatggatc cgacttcccg
     2461 tacgttgatc cgcgtccgta tcgatgatgc tgcgcaagct gaacgtcgag taaccacact
     2521 gatgggtgac aaagtagaac ctcgaagaaa atggatcgaa aatcacgtgc aatttacgct
     2581 cgaagaagat ggcagtatct tagataaaaa agaggatact gaaatctctc cttcagtttc
     2641 taatgattta ttagatgaag aacgagcaga taaaaatgaa aacaatcagt tatttgaagt
     2701 agaataggag gtcacctgat ggaaaatcga caagaagtac aagaattaac tttagaagaa
     2761 gtgatgggtg accgcttcgg acgttattcc aaatatatta tccaagaacg tgcactgcct
     2821 gatatccgtg atggattgaa gccagtgcaa cgccggatat tatttgccat gaacaaagat
     2881 ggcaatacgt atgacaaagg attccgtaaa tcagcaaaat ctgtcggaaa tatcatgggg
     2941 aattatcatc ctcatggtga catcagtatt tatgaagcga tggtccgcat gagccaagac
     3001 tggaagttgc gggaagtact gatcgaaatg cacgggaata acgggagtat ggacggtgat
     3061 ccacctgctg cgatgcgtta tactgaagcc agattatcca agttaagtgg agagatgcta
     3121 gcagatatcg agaaagaaac agtcgatctc gtgtggaatt ttgatgatac agaaaaagaa
     3181 ccaacagtcc ttccagctcg atacccaaac cttctcgtca atggatcgac aggtatatct
     3241 gctggatatg caacagaaat cccgactcat aacttagctg aagtgatcga tggcacaatc
     3301 tatatgatcg atcatccaca agcgagcctt gaaaaattga tggaatatat tcctggacct
     3361 gactttccaa caggagggat cttgcaagga aaagatgaaa tcaaaaaagc ttatgaaaca
     3421 ggacgaggaa aagttattct tcgttcgaaa acaaagatag aggcaatgaa gggtaataaa
     3481 cagcaaatcg tgatcacaga aatcccatat gaagtaaata aagcgacctt ggtgaaaaaa
     3541 atggatgaga tccgtttgaa taagaaaatc gatggaatag ctgaagtacg agatgaaagt
     3601 gatagaacag gtctgcaaat tgtcgtcgaa ttgaaaaaag acgccaatgc tcaaggaatt
     3661 ttgaattatt tgttcaaaaa cactgagcta cagatcaatt acaatttcaa tatggtcgct
     3721 atcgaccata tgactccgca tcaagttgga ctgaaagaca ttttaagaag ttatatcgag
     3781 catcgaaagc aagtcataac aaaacgtagt caattcgatt tagccaaagc tcaaaaaaga
     3841 cagcatatcg tagaaggatt gatgaaagct ttatcgattc tggatgaagt aatcgcaacg
     3901 attcgtgaaa gtaaagacaa aaaagacgcc aaaaagaatt tagtcgacgt ctttcaattt
     3961 acagaagaac aagcagaagc aattgtcaca ttgcaattat accgtttgac gaacacggat
     4021 atcacagagc ttcaaaagga atccgaaagc ttgattgcgc aaatcacaga gttgaataaa
     4081 atcctttcaa atgacaaaga gcttttctct gttatgaaaa aagaattgcg agaagtgaaa
     4141 aaaaattatt cttccgctcg tctaacgaca atcgaagacg aaatcgaaga aatcaaaatt
     4201 gatacgcaag tcttagtggc acaagaagac gtaattgtca gtgtgacgcg agaaggctat
     4261 gtgaagcgaa caagccttcg ttcttatagt gcttccaaac cagaagaaat cgggatgcga
     4321 gaaggcgact acttgctata ctcaggtgaa ttgagtacat tggaccatgt tcttctgatc
     4381 acaaataaag caaatgtgat ctaccgccct gtccatgaac tacccgattt gaaatggaaa
     4441 gatgctggtg agcacatttc tcaaacaatc tccaatctgt ctgtagacga atcgatttta
     4501 gctgttttcc catatcaaaa aattcaagca gagaaaacat ttgtattcat cagcaagaat
     4561 ggattgatca agcaaacacg aatgacagag ttcgaaccat ggcgcaccta taagagccgt
     4621 ccgctcagct gtatgaagct aaaggcggaa gaagatgaac tgattgctgt gtatctgaaa
     4681 gaagatcagc caggcttaga tgttttctta gtaacgcatc aaggaatggg tttgcgctat
     4741 cctttaacag atgttcccgt tgttggagca aaggcagctg gagtgaagtc tatgaatttg
     4801 aaagatggag ataggattat caacgggctg cttgtgttag cagaaggtga tacgccgatc
     4861 gtaatcgtta ctcagcgagg agctgttaaa cgcatgcttg ctcaagaaat cagccaaact
     4921 tcacgtgcga agcggggagt gacggtcatg cgtgagctga aaaaaaatcc gcaccgtatt
     4981 atctatatgt cggaaggaag atcaaaagaa ataaccttga tgaatcaaaa agggcagcag
     5041 ttaacgattg acccaactga tttccctata ggtgatcgaa catcgaatgg atcatttgcg
     5101 atggatgaga aaaaaggtgg agaagtcatc gaagttatcg atgctcctga gattttcatc
     5161 gagtcgtaac tgttgtagta ttttaatgaa gagacgccat ctgtattaat acaggtggcg
     5221 tctctttttc attcattttt aataaatcct tatacgtttg caaaaaaaga tgaaagtgtt
     5281 cacaatgctt aaatgttggt atgtcaacgt tgatttaaat atatgtaaat aaaacataaa
     5341 aattgttaaa taaaaaacaa gaaaaccctt gcaaagatag ggtgctgtgt tatactaatc
     5401 acgtaaaaac tgttacataa aacagaaatt taggaggtcc atacaatgga gcaatggaat
     5461 ggatttaaag gtaccaaatg gaaagaaggc gtagacacta gagactttat tcaaaataac
     5521 tacactgaat atcgcggtga tgacagcttt ttggaaggcc caacagaagc aacagaaaaa
     5581 ttatgggatg aattgcaaaa gttaaatgat atccaattcg agcgtaacgg tgtctatgat
     5641 atggacacag aaatcgtatc aactattact tcacacgatg caggctattt ggataaagat
     5701 ttggaaaaaa tcgtcggtct acagacagac aaacctttga aacaagcatt catgccgttc
     5761 ggcggaatca atatggctaa caatgcgcta aaaagcaatg gctatacacc aaacgatgaa
     5821 ttgacacata ttttcactga ctggcgcaaa acacataatc aaggtgtatt tgacgcttat
     5881 actgctgaaa tgcgtgctgc tcgtaaaaac aaaatcatta cagggcttcc agacgcttac
     5941 ggtcgcggac gtatcatcgg tgactatcgt cgtgtggcat tgtatggaat cgactatttg
     6001 atggaacaaa agaaaaaaga tcatgatatg actggttatc atacaatgag tgaagaagtg
     6061 attcgtttac gtgaagaaat cactgaacaa tatcgtgcat tagcgcaaat gaaagaaatg
     6121 gctgcgaaat atggctatga tatttctaaa ccagctgcaa atgcaaaaga agctgtccaa
     6181 tggctatact tcggatattt agcagcaatc aaatcacaaa atggtgctgc gatgtcaatc
     6241 ggacgtatct cagcattcct tgatatctat atccaacgtg atttggataa tggagtaatc
     6301 acagaaaaag aagcacaaga attgatcgac cacttagtaa tgaaattacg tatggtcaaa
     6361 ttcgcgcgta cacctgaata taaccaatta ttctcaggat tccctatctg ggctacttta
     6421 tccattgcag gtatgggatt agatggtcgt tcattagtta caaagaatga tttccgtctg
     6481 cttcatacat taacaaatat gggaccttca ccagaaccaa acttgactgt tttgtattct
     6541 gaacaccttc cagaaggatt cagaacatat gctagcagaa tcgctatcga aagttcttcg
     6601 attcaattcg aaaatgataa tcagcttcgt gaacaatggg gttctgatga ttgtgcgatt
     6661 gcttgctgtg tatcaggaac agttgtcgga aaagacatgc aattctttgg cgctcgtgct
     6721 aacttggcta aaacactatt atatgccatc aatggcggag tcgatgaagt gactggcgcc
     6781 caagttggac cagaatatcg tccaatcaca tcagaaatac tagactttga agaagtgaaa
     6841 cataacttca tgaatatgat ggattggtta gcagaactat atgtcaatac gttgaacatc
     6901 attcattata tgcatgataa atattcttat gaagcagctc agctggcttt gatggattct
     6961 gacttgcaac gcacatttgc tacaggtatt gctgggatct ctcatgcggc agacagcttg
     7021 tcagcgatca aatatgcaaa agtaaaagca atccgtaatg aaaaaggaat agcagttgat
     7081 tttgaagtgg atggcgactt ccctaaatac ggaaatgatg atgaccgtgc agatgaatta
     7141 gtttcatgga tcttggaata cttcatggga caaatcaaac gtcataaaac ttaccgcaac
     7201 tcaaaaccaa caacttcatt attgacgatt acttcaaatg ttgtttacgg taaaaataca
     7261 ggaaatactc ctgatggccg tgaagctgga aaaccacttg cgccaggagc aaatccaagt
     7321 tacaatgcgg aacaaagtgg gttattagct agcctgaact caacagctaa aatgaattac
     7381 ggctgtgcat tggatggaat ttcaaatacc caaacaatca atccttcagg tcttggaaaa
     7441 gaacaagata caagaatcga aaacttgcgt aatgtcttag atggatactt ctcaaaagga
     7501 gcttatcatt tgaatgtcaa tgtattcagt aatgatttat tgcgtgatgc aatggaaaat
     7561 ccaatgaaat atccgaactt gacgatccgg gtatctggtt atgcagttaa attccgtgac
     7621 ttgacaagag aacaacaatt agatgtcttg atgagaactt cacacgacag aatgtaaaaa
     7681 gttcgtgaaa aaagtttttc gacttagcgt cgaaaggacg ttgaatagcc atcgcatcaa
     7741 aatcgaataa tcagtatgct tatttgaaag aaacgattta caggggcagg acttgtatgt
     7801 tccgtccctg taaattttac acataacgaa gcggcaattg cgaaaaattt tgttcagaaa
     7861 gcttcgcttc gacagctaac ttgtacttaa aatgaaatca aagaaaagat gtgaacttaa
     7921 atggaagaaa aaacaatcgg ctatgttcat tctatcgaaa ctttcggttc agtagacgga
     7981 cctggactgc gctttgtcgt gtttatgcaa ggatgccgca tgcgttgcca attttgtcac
     8041 aatccagata cttggaatat tggcggaggg aaagaataca cagcagatga acttttagat
     8101 aaagcagaac gcttcagacc ttactgggga gataaaggtg gtattactgt tagtggtgga
     8161 gaacctctat tgcagatcga ttttttgatc gaactattta aaaaagcaaa agagcggaag
     8221 atgcatacta cattggatac atgcggaaaa ccttttacgt atgaagaacc tttcttttca
     8281 cgttttcaag aattgatgaa gtatacagat ttactgttat ttgacatcaa acacatcgat
     8341 aatgaagagc ataaaaaact aacgcattgg gacaatgaaa atattttaga aatggctcaa
     8401 tatttgtcaa aaatcaacaa accggtttgg attcgccatg tactagttcc tgagcgaagt
     8461 gattatgatg aatatttgat ccgactagac aactttattc aaaccttgtc aaatgtagac
     8521 agagtagaga ttcttcctta tcatacgatg ggaaaataca agtgggaaac acttggtttg
     8581 aaatatccat tagaagggat cgaaccacct acaaaagaac gtgtcgaaaa tgctaaacga
     8641 ttgcttcata cagaagagta tacaaaatat ttaacgcgat aggtcgcatc ggaaagaaga
     8701 taagtattgc ttctttccgt ttttttgcta ttattaaata ggagaaatca caaaggagcg
     8761 aatgacatgt caaaaatttt agtttttggt caccaaaatc cagatacaga tgcaatcggg
     8821 gcagccattg cgttttcaca cttgcaaaat gaattaggta aagaaacaga agcagttgct
     8881 ctaggtacac ctaatgaaga aacacaatac gcattagacc actttcagct atctgcacca
     8941 cgagtagtag agtcagctaa agcagaaaat gcagatgtca tgcttgtgga tcataatgaa
     9001 ttccaacaaa gtatttcaga tatcagcgaa gtaaatattt tagctgtagt agaccaccat
     9061 cgtattgcaa actttgaaac agctaatcca ttgtactatc gtgcagaacc agttggatgt
     9121 acaagtacga ttattttaaa actatataaa gaaaaccaag taacaattcc taaagaaatc
     9181 gcaggaatca tgctctcagc tatcatttca gatacattgt tgttcaaatc accaacatgt
     9241 acgcctgaag atgaagcagc tgcaaaagaa cttgcagcaa tcgctgaagt cgatctaaac
     9301 acttatggac ttgaaatgtt gaaagcagga acaaatctaa gtgataaatc agctgcggtt
     9361 cttcttgatt tggatgcaaa aagtttccca atgggcagtt ccaacgttcg tatcgctcaa
     9421 gtcaatacag tggaccttaa tgaagtgatg gatcgacaag cagagctaga agcagcaatg
     9481 gaagatgaaa tgaaagcaaa tggctacgat ctgttcgtat tagttgtcac aaacatctta
     9541 gacagtgatt cagaattact tgttgctggt aaaggaaaag aaaaagtaga agaagctttc
     9601 caaacaactt tgaccaacaa ccgtgctttg cttaaaggtg tcgtttcacg taaaaaacaa
     9661 gtcgtacctc aattgacggc tgtatttagc taatcaaatg gtagtatgaa gcattcaaaa
     9721 tgaatggata agcgattgat ataaaaagaa acatagaaca tactaaaggc cgaagaaatg
     9781 gaggagaata acagtcaatt atgactcttg ttcttcctcc tttttacgta aaagcctttt
     9841 aagtaaaata aaacggatag aaaagagaac atatatgaac tattattcaa aaaataagga
     9901 aaacttttat caaaaactga ccgatgatcc gcttttttct tcattgaccg attatctata
     9961 tgaacatcga gaacaagaaa cgattctaag agaattgaaa aaagaatttt cgcaaaataa
    10021 attttcccat tttcttgatt tattgatcga tgcaggatta atcaagaggg aagaacgaag
    10081 atatcatttg aactttccaa tatttgatcc taaagattac ttgcaacaag ccacttcggc
    10141 tgctgaaacc atagctgaac aattgaagag actctctgtt gatgaacaaa aattagcgat
    10201 gggagaagtt atttgggcgt attgtttcga agatgaacgg aaagaagcgt atttttatgg
    10261 agtaagaaac agccgagaga cagaattgct gaggacaact gcaggaaatg agaagtaccg
    10321 ttttatcaca ctttcctcta tagaacactt cccgcttact ttggctaatt acttttttgt
    10381 acagaagaat cagcttcctg taacgaaggc attcaaagaa ttagccgaac ttattggaga
    10441 cgtaaatgaa tcgtactttt ttgatcaaat agaagttatc gttgacagga tacgtaagaa
    10501 taaatataaa aatcgccggc ctagtatttt ccatcagtct ctacttgtga cgaatactat
    10561 aaaggaagaa gaatcattca cattagaatt acctattgtc gaaaaaaaca attttgagat
    10621 tgaattacca acattggatc cttcattgac aatggaggaa atagcctttc ttaaacgaca
    10681 aatcttttct gagctgagca agaaatttat accgcatgca ttttcttata tcaaagaata
    10741 tggaacagtt ttagtgagta aaacctaaga gagcagtaag caaaaattat actgactctt
    10801 gcaggaggag cctttctctt cctttatggt taaaagaatt atttgagtat tttgcctatc
    10861 taccacctat tagattcact acatggtctg gaaacaaagg aatgcaaaaa aatggtgaga
    10921 aatgtggaat gacaaaggaa gtagtgataa gaaaagttag atttttaaat aatcaatatt
    10981 atgattcagt caagtatggc attttatggg aagaactagc tgattagatt aggaagtcca
    11041 tttgataatc cagaatcact aagaaacgaa tggcgagtca ttaataattt atccaatctt
    11101 gggttattct tttagattga agtgtttaaa actaaaaaag catttatgaa gtcgtaatag
    11161 tcaggcttca aaatgctttt tattttgttt gttttttctt tgccacataa atacacctag
    11221 actgacgatg aagagcatat ccaacagatt tcctgcgact tggatcgtcg aagggttggt
    11281 cagtggcatt aaagcaagga caaaaaagga aaacaagctg taaataaaac aggtgacagg
    11341 aactaatcga aagaagagcg gacatttcaa ttggctaaaa atgagtgaaa aaataaaata
    11401 agtaaaaatg gataaaatca cgactaaacc attcatgata aagaagaaaa gcgtattgtg
    11461 attaaacgaa taaaaaagaa ttgctaaaaa agaaaacgtt ttaaatgcaa tcaatacgct
    11521 tgaagcttcg tttaacgtac caggatgtcg atgaaaaaaa taggcaccga aataaacaga
    11581 aaacagtaag ccgcaaagag aagacaaaat caaaaaaaac ggattttctt cggcttctgt
    11641 gatatcagaa tgatccaaaa gcaaccagcc taatgcacta gaaggcgcat gtgaggtctt
    11701 aaggtatgaa taacatagaa aaaataagat cgtcaaagtt gcaaaaaaag agcctaaatg
    11761 taaaaaatta gtggttctac gttgaatttc cataatatct ccaaattaaa ttgataagta
    11821 tataaagtat aataacaaat tgatgagtat atgtcgatgt tttttaatca aaataataaa
    11881 taaaaactgt ctaacattta aaatttaatt tagatattag acagttttta agtttgtgag
    11941 ttattttcct tgtcgttttc tcactttttt agtccagaaa ccacctgaaa tatattttgg
    12001 ctcataagaa atgataaaag cacgctcttc gatttctttg atcaggcgat acagttcacg
    12061 ttcgcttttt ctaggagaaa gtatttcgag aactagccgc tcaccttcca gccccattgc
    12121 agaagtctgt gtcacaccat aaccgtgttc tctcagtata cccggcaaac ttttatcttc
    12181 tgcgctattt gctggcaaaa tcgttgtgac catgatgtat cctaatgcca atttgtcttc
    12241 aatcaaaatt ccgacactga tacctactgc gtaacctaac gcatagacaa ataaattcag
    12301 tggattatct aaacgatcta aaacaaggga taagccgacg atataaatcg tgatctcgac
    12361 catactgaca agtggagcaa tcagtcgata gcctttcatt gtcaacatga aacgtaacgt
    12421 gttcagagtg atataagcaa aattaatgag gaagatcatc cccaacattt ttggatcaat
    12481 catttgtaaa actcctttca tttatataac aacaatatca aaaaaaatcg taagtgaaga
    12541 aaaattgaaa ctttatacat acaaaaaaat gataaaatga ttataataga attataaaga
    12601 taggttaggt gattaaaatg aaaacggtca atcgtgtgtt ttgtgccagc cacgtgctaa
    12661 gtctccttct cagtatatac ttaggtgtct attcgattca acagccaatt gaggtagaga
    12721 acattgtttt tcagatgtcg ctgacagatg gtttaatttt aagcgttttt ttctttttga
    12781 tactgtttat tggtaatatt ttcggtagcg ttgccagttt tttgaatttt tctatttatc
    12841 cactcctttc gctagcatta ggagtggtgg gactttgttc tctgtgctta tttcccgagc
    12901 cattcagtcc tgcatttata ttatttggta cactgaacct gttccagaca actgttggag
    12961 catggttgct atggcgatca ggaaatttaa tgaaaatagg tgagtaacaa ttgaatccaa
    13021 ttatattgaa acgttctgtc catcatattc cagtgttaga agttgttctg aaagaattaa
    13081 gagaggaacg tattcccgtt gtcatctatt atcacggatg gcagacaagt aaggagttag
    13141 ttttgacgca aggaagaaaa attgcgcagc agggcattcg tgtgatcctg ccagatgcga
    13201 tgaatcatgg ggagcgcaaa cagccggttt cttctattcc atcctataca ttttgggata
    13261 gtatctacgg aaatctgttt gaatttgaca cacttatcaa gcatttagaa aaaagaggtc
    13321 ttctacatga tcagcttgca gttggcgggg tttcgatggg gggaatgaca acatgcgcct
    13381 tgttggctaa acatcctcat atagtagcgg gcgtgtgttt gatgggttcc cctgcacctt
    13441 tggaatatgg aaaagaaatc gtaaggcgcg catcggaata taattatgtc gtaccaaaag
    13501 actattttga tttgatttca tgggttaaga gctatgattt gtcattgcag ccggaaaagt
    13561 tagctggacg tcctctgttc ttttggcatg gacaagctga cgaaaaaatc ccttatatac
    13621 aaacggaacg atttgtagaa aaaaatcggc ataagtctta cggaaaagaa attgtttttc
    13681 gttcctcaaa aacacagcga catatggtac aaataccact aatggaagaa gcttccactt
    13741 ttttagcaaa aaatttgata aaaaagtata aataattgaa acggaaaatc actcatgacg
    13801 atagaggatt ttccgttttt tattttgggc tataataaac tctgttagga aaatttggta
    13861 taattgatag taagaatgac aacatgttag aaaggaagag agcatgccca ctttagcaga
    13921 tgttgcaaaa cgtgccaatg tttcaaaaat gacggtctct cgagtcatca atcatccgga
    13981 acaggtgact gacgaattga aagagttagt tttctcagca atggctgaac tggattaccg
    14041 tccaaatatt gctgcaaaag cacttgtcac gaatcggtcg cagatcatca aattatttat
    14101 actggaagaa atcgacacaa ctgagcccta ttacatgaat ttgctgatgg gaatcgctaa
    14161 acggatcggt aaagcgcatt attctctcca attagttaca aatgatgcct tcgacgtcgg
    14221 ttcttgtgat ggatacatca tcacaggtgt cagaaaaaaa gacttcgatt ggatcgaccg
    14281 tttggaaaaa ccagttgtct tgtttgggga aaattctgaa ggatatgatt atgtagacag
    14341 cgataatgaa tatggtacag caaaagcaac gcaatatgcc ttagaaagag attatcaaac
    14401 gattatttat atcggtatcg aagtggacga gccttttgaa atttcaaggg aaactggtta
    14461 tttatctgtg atgaaggaag ttgatcttcc tagtaaaatc ttgcggtttc caaatcattc
    14521 gacagaagta gaaagatata tgacagaaaa ctgggcagat tttacagata aaacttgttt
    14581 tgtatgtagt tcagatcggc tagcactagg agcagtaaga gggattataa atgctggagg
    14641 acacttgccc gaagaatttg gtgtgattgg atttgatggg gtatttttag accaaatcag
    14701 ttcacctaaa ttgacaacga tcaagcaaga tattatacgt atgggtgaag tatgtggaga
    14761 aatgttgtta aaaaaaatag aagaaaaagg ggcgagtcaa ggttatcgtc gtttttttcc
    14821 tgaactagtt atcagag
//
LOCUS       DAZKNM010000059        14682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000059 DAZKNM010000000
VERSION     DAZKNM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.059"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(209..1288)
                     /locus_tag="ACG8M9_001444"
     CDS             complement(209..1288)
                     /locus_tag="ACG8M9_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0378228.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            1475..2173
                     /locus_tag="ACG8M9_001445"
     CDS             1475..2173
                     /locus_tag="ACG8M9_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0378229.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2341..4980)
                     /locus_tag="ACG8M9_001446"
     CDS             complement(2341..4980)
                     /locus_tag="ACG8M9_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0378230.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(5599..6630)
                     /gene="queA"
                     /locus_tag="ACG8M9_001447"
     CDS             complement(5599..6630)
                     /gene="queA"
                     /locus_tag="ACG8M9_001447"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0378231.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            6823..7997
                     /locus_tag="ACG8M9_001448"
                     /pseudo
     CDS             6823..7997
                     /locus_tag="ACG8M9_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8001..8642
                     /locus_tag="ACG8M9_001449"
     CDS             8001..8642
                     /locus_tag="ACG8M9_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378232.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            8639..9556
                     /locus_tag="ACG8M9_001450"
                     /pseudo
     CDS             8639..9556
                     /locus_tag="ACG8M9_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            9553..10224
                     /locus_tag="ACG8M9_001451"
     CDS             9553..10224
                     /locus_tag="ACG8M9_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378233.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(10765..11253)
                     /locus_tag="ACG8M9_001452"
     CDS             complement(10765..11253)
                     /locus_tag="ACG8M9_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0378234.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11454..11720)
                     /locus_tag="ACG8M9_001453"
     CDS             complement(11454..11720)
                     /locus_tag="ACG8M9_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0378235.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(11720..11983)
                     /locus_tag="ACG8M9_001454"
     CDS             complement(11720..11983)
                     /locus_tag="ACG8M9_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0378236.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12504..12644
                     /locus_tag="ACG8M9_001455"
                     /pseudo
     CDS             12504..12644
                     /locus_tag="ACG8M9_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(12786..14609)
                     /locus_tag="ACG8M9_001456"
     CDS             complement(12786..14609)
                     /locus_tag="ACG8M9_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0378237.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4170 a         3209 c         2908 g         4395 t
ORIGIN      
        1 tttaatagaa tttataagcg taaatttcaa gtttaagtta gccacccatg gtgacccggt
       61 tgctcgttac caccaaccgt ctaatttttc taatcgagtg ggcccttgac aatgagctga
      121 ggcatggtag tctcccgact ggctggcccc ggccagcttt ccagacacaa gccatgccga
      181 gggaggctcg ttgtcaaggg cccacgtgtc aagccgtcaa ccgttcctgc ctaaatgcct
      241 ggacgaacgt gtcatgcggc gttgcatagc ccaaaatctt tcgcgggtaa tcgttcatcc
      301 aacgctgaat ccgcaagatt tggttttcgc tcacgttgcc gattgggctg cccttgggga
      361 tgaaacggcg aatcagcttg tgttggtttt cgctcgtccc gcgctcccag gaagcatagg
      421 ggtggctgaa gtagatgttt gccacatcct gcaaggcatc gtgcagtccc gcgaactccg
      481 agccgttgtc cgaggtaacc gtcttgaaca gctggccgaa gtacgcacca gcccgttctc
      541 tcagctgtcg gaccgcttgg ttgaccgatt cggcatcctt cccggccagc ttcagaatga
      601 cctcgaagcg tgtctgccgt tccaccaacg tcagcagcac ggcatccgac ttggtcttgt
      661 ttcctacgac cgtgtcgact tcccaatggc cgaaggtttt ccgggtacca accgcctccg
      721 ggcgctcttc aatggatttc cctagcaccc gtttattcgg gcggttgcca tgacgggacg
      781 atttcggttt gcgggacagt ttttctagca ggtccatatt cttcgttttc atgatgccac
      841 ggtcgatcca ctgatagagg gtggtggtcc cagggataat ggccgggtcg aacaattcgt
      901 gttttctggc aaagcccacc accacgtccg gggaccattt ttccaacagc atcatgtcgt
      961 ccgcccattc aatgaaggcg tccgtgtcgg cccataaggg ccgtctgccg cagttcaagc
     1021 ggttcttctc gtaggccgcc tgcccggcat cggcgtcgta gacgtcatga taatagtcgt
     1081 agaccttccc gttctgattc tggcggttaa gctgagtaac ggtcccgcgt ttgatttcgt
     1141 tgtttatggt ctgcggcacg cagcccagga catccgcaac ctgacggttg gaatagtttt
     1201 cctttttcag gatggcgatt tggcaacgtt ctgagtaggt taggtgtttt cccttgcgtg
     1261 aaactgtgtt atcatggttt tgcgtcatgt gaattcatcc ttgtctattg agagttgtgg
     1321 taacttcaat ataacatgga attcacgtgg cgttttttta tttccaaatg gctaagttaa
     1381 ttataaaatc cgccaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta
     1441 tattggtgtt attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg
     1501 ccaagcaaaa gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc
     1561 tacattgatt ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac
     1621 caccatatat gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc
     1681 aggaggcggt ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc
     1741 ttggacttat cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc
     1801 atttcgtgga ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc
     1861 gatctttaca acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc
     1921 ttattctcaa agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa
     1981 agtactggat acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt
     2041 ctggttagat ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg
     2101 ttatctatgg ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc
     2161 aaaagatatt taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt
     2221 atcgagaggc agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc
     2281 atgtcacacc tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa
     2341 tcattcctct tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat
     2401 tggtacgata gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc
     2461 gaatagttct tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa
     2521 cgccatttct accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct
     2581 gcgtacattg aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg
     2641 accgacatgc tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata
     2701 ataaccaaac aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa
     2761 acctcgagca aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc
     2821 cgctggctct cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag
     2881 tagttgcaca gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa
     2941 gatctccgaa atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac
     3001 cgaacgtcct tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat
     3061 atctgcggca ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact
     3121 tgctttcaac gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt
     3181 acgttgccaa gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga
     3241 gagagctttg acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga
     3301 aagtgcttct gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac
     3361 cacatgatct ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc
     3421 tcctttactt tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc
     3481 tttttccagt gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac
     3541 agcaatcact ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac
     3601 atcgcctgaa ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta
     3661 tttttcgccc atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc
     3721 gtttactcgt ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc
     3781 ccttacgatg gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc
     3841 gaccgtcaca tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat
     3901 tgaatcttcg atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc
     3961 attttgtgtc agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc
     4021 aactgctggc aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc
     4081 aagtgtcaac gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat
     4141 cgaagtcatg aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa
     4201 agccgcagcc gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg
     4261 taaaggtgtt ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt
     4321 agccatgcca atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt
     4381 aaataccata ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc
     4441 tttttcaact gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc
     4501 ttcgatcaaa cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc
     4561 cactaccagt tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt
     4621 ttgtttattc atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat
     4681 cgccaatacc gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc
     4741 atgttctgta gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt
     4801 tgtaaaatcg gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc
     4861 gaatttattc aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt
     4921 atctggatct gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat
     4981 cctattcctc ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt
     5041 tttttattta cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa
     5101 agtgcttata tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga
     5161 taccatcaaa tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag
     5221 aagtacaggt ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta
     5281 tggatgtttg gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat
     5341 aacgtaaacg tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac
     5401 gaaaacaaaa tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg
     5461 aaagttacaa tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat
     5521 cgagagctcg gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac
     5581 tagcggcttt tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata
     5641 gcgctcttca atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact
     5701 aactaacatg actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac
     5761 tttgaattca tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc
     5821 tccattaaat ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac
     5881 gattttcccg ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg
     5941 gtagaattca ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt
     6001 acctaaccct acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc
     6061 aagtaattct ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt
     6121 cgcatagaca gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa
     6181 tggcatttct cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat
     6241 gatccgtccg ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa
     6301 agagattctt gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc
     6361 tccttgtgta ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc
     6421 atataaacga gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa
     6481 ctcttcaatg atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac
     6541 aagtaaacga gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa
     6601 gtcaaaatca aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca
     6661 tcattctatc atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt
     6721 gttttcctaa ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt
     6781 aatagatagt tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat
     6841 gtgtcaaaaa tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa
     6901 aagggtgagt tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg
     6961 atgatcaacc gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc
     7021 caagaaattg atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga
     7081 ttgatgcctc acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc
     7141 agtcaagaag aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt
     7201 gagatgttag atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc
     7261 gttcgtgccc ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt
     7321 gatccaatca ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga
     7381 aaaacgatcg tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc
     7441 gccatcatga gtgaaggccg tgtgatcaat ttgatacacc agaaaatatt ttacgtcatc
     7501 ctgcaaacga tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa
     7561 atgcgacaag agtcggcgaa gttatgctaa atacagcaat cacgattaca ccggaaaaat
     7621 cattacaaga agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg
     7681 ataattcgaa tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aaacgaggaa
     7741 aagtatccag tgtcggcgac attttgaata aacatgtttt ttacgtaaaa gaatccgcct
     7801 atcttcgtga taccttgcaa cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg
     7861 atgaacagaa caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg
     7921 atgtgatttg gggagaagat cccgcaacta ctgatgttgc agagtcagct actcctgaag
     7981 aatctaagga ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct
     8041 caaaaggttt tgaacatctt tatatttcag gacttgcttt gatcattggt atcatcattg
     8101 ctgtaccgct tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct
     8161 taaccagtgc cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat
     8221 ttggcgtagg taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt
     8281 tacgtaatac gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag
     8341 gcatggggat gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa
     8401 cgatcatggc tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct
     8461 cttatatcgg cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac
     8521 cagatctcat tttggcaggt acgatccctg taacaatttt agcactattg gctgattggc
     8581 ttctaggtct cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat
     8641 gaaacgtata ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc
     8701 cttccctggt cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac
     8761 ttcagaagca cagatcttag caagtctagt tgccggaatg gtttaacatt atacagataa
     8821 aaataccgca atcatcaaca atctggctac tacaactatc aaccatcaag cgatgatgaa
     8881 cggagatgct tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa
     8941 ccttcctcct gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa
     9001 aagatacggt caaacgtggt tcccgtccta tagttttgaa aatacgtatg tctttcttgt
     9061 tcgaaaagac accgcacaaa aataccatct atctaaagtc agcgatctga aaaatgtagc
     9121 ggatgaatta gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga
     9181 cggcttccaa gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact
     9241 tgtttatgac gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg
     9301 acggatcgcc agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata
     9361 tgatgctgct ccagttgtag acaacaagat attagctgat acgcctcatt taaaagaagc
     9421 cttaaataag ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc
     9481 agataataat ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta
     9541 ttttgaaggg aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat
     9601 ttccaggaaa atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc
     9661 tatggtgtcc tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga
     9721 cgtaaattag caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta
     9781 gccatgatct ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact
     9841 gtatttttgt attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta
     9901 gacaaaaaca tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc
     9961 atggtagagt taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt
    10021 gttgccatcg ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc
    10081 attatccgag gaaccaatgc aacagagggc acatcgatta ttttagcagg tgcattgcca
    10141 actgcattaa tggcgatcat cacggactgg cttttaggta ttttggaaag acggctagat
    10201 ccagccagcc gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt
    10261 gttggtcgca aaggcttttt tagttctgtc atttttaagg caataatcca ctctgattct
    10321 tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg tatactccaa
    10381 cttcatactg ccaacgggtt catatctgca tccaccgact aagttttttt cttatccgct
    10441 aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc acatgatctc
    10501 taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct ttggcgtggc
    10561 tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct gcatcatcaa
    10621 aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg aacttgccat
    10681 cctcatcctt ggctaaaaac atatctagcc attccagctt ctcgccatgg gaggtttgcg
    10741 gttcctaaaa ccggtacacg attcttatgg actgcatccg tcgcaactag acttggcgat
    10801 acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat attgccaata
    10861 agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt tgatactgct
    10921 attcatggcc gaaagcgcca cgactttaac ttcttccgtt tgagtgacat tcatgttcgg
    10981 caatgtcgct ttggataccc gctctgctaa aggaacgaca ctggtattgt atttcgtatc
    11041 tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag acgactccgg
    11101 ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa cgacatttcc
    11161 aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca acactaacaa
    11221 cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt ttacataaac
    11281 ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag atataggaac
    11341 aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa aaaagagtct
    11401 cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc acttcactca
    11461 taatgtgtcc aggcagaata aatttctaca actttatttt cctcatcaac tttgtaaacc
    11521 actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg tctcagcttt
    11581 tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac ttcttcaaat
    11641 tgtttcttta aattcgtttg cttgattttt cttaaatcca ctttcgcaga gtttttgatt
    11701 gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata ttcgtaaagc
    11761 tactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa
    11821 gggtttcttg aatcgctctc cagtcttcca gcccaatcaa tacagcattg tttccactac
    11881 gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat aactgataaa
    11941 aatttttacg agcagtgcta ggattcatcg ttgtattagc cattgtctct cctccttttt
    12001 ctgatataat tatagcgtac ttgattaagt acgtcaaaaa aatacaataa agcacttaaa
    12061 ttcagcaaac ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt
    12121 tgtccacgct cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt
    12181 aacaacgtat catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac
    12241 caatacacga aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga
    12301 tacgcacgaa agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact
    12361 gcctcaaaaa tgattgcctc tttcgtctcc tgtcattcat gttcactatc gatttgatac
    12421 ttgcttagac cgttgggttt gtgaatcacc aattaaaaat ggcactgagt tatggtaaca
    12481 aaaaatggtc acttgacgag gctgagattc ctacttatga agacacatgg cacttaagag
    12541 tcgaggtaaa ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg
    12601 gaaattatga tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa
    12661 catttcatac gctgttccca tctttaatgg cttatagagg gcacctttag ctgagaataa
    12721 gacaaaacag accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac
    12781 ttggtttatt tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac
    12841 aatcgcaatc ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt
    12901 ggtacaaatt gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt
    12961 acgatatcta cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt
    13021 gaaaaacgga catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg
    13081 acatcttcgt ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa
    13141 ttcaacgcac cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg
    13201 tcataatcat gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa
    13261 tgatcatgga tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa
    13321 atgtccccta accgatagac aaacaaaatc gcaatgattg catatgagat gaacgcaccc
    13381 gtaatattgg caatcgattt gcttaaccat ctttttcctt cttttttcca tttgacgacc
    13441 atccctgttt gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag
    13501 cgttctgttg agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata
    13561 atcccattgg aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta
    13621 tcttttgcca gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact
    13681 gctaaaatca gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca
    13741 gctttaccaa tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag
    13801 ttgataaatg tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta
    13861 gcagcggcat tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc
    13921 accccagtta aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca
    13981 cccggcacag ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata
    14041 ttatataaac caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat
    14101 aataggaacg atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg
    14161 attgtcacag aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct
    14221 tcagcacctg ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca
    14281 ccggaaatca agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg
    14341 ccatgaggat aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata
    14401 ataacaaaag cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa
    14461 acgacaacga tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat
    14521 gccaaagcag caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta
    14581 cctactaaca acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt
    14641 caagttttat agtcgaaaat ttatagtaaa caagatgtta ta
//
LOCUS       DAZKNM010000060        14549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000060 DAZKNM010000000
VERSION     DAZKNM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.060"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8M9_001457"
     CDS             4..816
                     /locus_tag="ACG8M9_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0378238.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8M9_001458"
     CDS             813..1613
                     /locus_tag="ACG8M9_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0378239.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8M9_001459"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8M9_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0378240.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8M9_001460"
     CDS             3110..4819
                     /locus_tag="ACG8M9_001460"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0378241.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG8M9_001461"
     CDS             5031..9389
                     /locus_tag="ACG8M9_001461"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0378242.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8M9_001462"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8M9_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0378243.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8M9_001463"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8M9_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0378244.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG8M9_001464"
     CDS             11227..11520
                     /locus_tag="ACG8M9_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0378245.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG8M9_001465"
     CDS             11517..11828
                     /locus_tag="ACG8M9_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0378246.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG8M9_001466"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG8M9_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0378247.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8M9_001467"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8M9_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0378248.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DAZKNM010000061        13689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000061 DAZKNM010000000
VERSION     DAZKNM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.062"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="ACG8M9_001468"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="ACG8M9_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0378249.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="ACG8M9_001469"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="ACG8M9_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0378250.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="ACG8M9_001470"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="ACG8M9_001470"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0378251.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="ACG8M9_001471"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="ACG8M9_001471"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0378252.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /gene="rpsF"
                     /locus_tag="ACG8M9_001472"
     CDS             6088..6387
                     /gene="rpsF"
                     /locus_tag="ACG8M9_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0378253.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="ACG8M9_001473"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="ACG8M9_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0378254.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /gene="rpsR"
                     /locus_tag="ACG8M9_001474"
     CDS             6975..7211
                     /gene="rpsR"
                     /locus_tag="ACG8M9_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0378255.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="ACG8M9_001475"
     CDS             7354..9327
                     /locus_tag="ACG8M9_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0378256.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="ACG8M9_001476"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="ACG8M9_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0378257.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="ACG8M9_001477"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="ACG8M9_001477"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0378258.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12206..13498
                     /locus_tag="ACG8M9_001478"
     CDS             12206..13498
                     /locus_tag="ACG8M9_001478"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0378259.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4561 a         2477 c         3014 g         3637 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgtaaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatc aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggtgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagaca ttgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatggggaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ccgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc cttgaaaact
     4441 atatcgaaca tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa gaaaaagcag
     4501 aagcaagagc acacatctta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa agcaacaatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgtaca gccaaaggaa tcccaatcat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggtcaga gggatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaaaatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat aaccatcaag actaagtgtg taagctagca
     5881 agaataaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatt
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattagat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca atgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatattgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    12001 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    12061 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    12121 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    12181 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    12241 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    12301 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    12361 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    12421 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    12481 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    12541 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    12601 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    12661 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    12721 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    12781 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    12841 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    12901 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    12961 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    13021 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    13081 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    13141 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    13201 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    13261 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    13321 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    13381 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    13441 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    13501 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    13561 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    13621 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct
    13681 ttttccgaa
//
LOCUS       DAZKNM010000062        13663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000062 DAZKNM010000000
VERSION     DAZKNM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.063"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..573)
                     /locus_tag="ACG8M9_001479"
     CDS             complement(<1..573)
                     /locus_tag="ACG8M9_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0378260.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTI"
     gene            complement(821..1657)
                     /locus_tag="ACG8M9_001480"
     CDS             complement(821..1657)
                     /locus_tag="ACG8M9_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0378261.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1668..2597)
                     /locus_tag="ACG8M9_001481"
     CDS             complement(1668..2597)
                     /locus_tag="ACG8M9_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0378262.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2789..3142
                     /locus_tag="ACG8M9_001482"
     CDS             2789..3142
                     /locus_tag="ACG8M9_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0378263.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3216..3872)
                     /locus_tag="ACG8M9_001483"
     CDS             complement(3216..3872)
                     /locus_tag="ACG8M9_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0378264.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4098..6032
                     /locus_tag="ACG8M9_001484"
     CDS             4098..6032
                     /locus_tag="ACG8M9_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0378265.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6118..7341)
                     /locus_tag="ACG8M9_001485"
     CDS             complement(6118..7341)
                     /locus_tag="ACG8M9_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0378266.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7335..9206)
                     /locus_tag="ACG8M9_001486"
     CDS             complement(7335..9206)
                     /locus_tag="ACG8M9_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0378267.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9227..10345)
                     /locus_tag="ACG8M9_001487"
     CDS             complement(9227..10345)
                     /locus_tag="ACG8M9_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0378268.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10428..11261)
                     /locus_tag="ACG8M9_001488"
     CDS             complement(10428..11261)
                     /locus_tag="ACG8M9_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0378269.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11254..12171)
                     /locus_tag="ACG8M9_001489"
     CDS             complement(11254..12171)
                     /locus_tag="ACG8M9_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0378270.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12194..12373)
                     /locus_tag="ACG8M9_001490"
     CDS             complement(12194..12373)
                     /locus_tag="ACG8M9_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378271.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12398..13522)
                     /locus_tag="ACG8M9_001491"
     CDS             complement(12398..13522)
                     /locus_tag="ACG8M9_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0378272.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4265 a         2726 c         2243 g         4429 t
ORIGIN      
        1 aattgtgccg tttacgagaa tctcttcatc tttcaatact aggcctattt tatttttata
       61 acctggcatg ctcattagat aaaggatttc atcgtaatgg atatagtgat cgctattacc
      121 ataacggaca gttgtccacc tttttttcgt agcatgttga acaggatagt tttctttttc
      181 tttcaataag taactttgga tgcttttttt atttttttca taagtgaatt cttttatcaa
      241 ataaccacta ggcgtaatct ttcgattaat aacagttatt gctttattcg gatgagacgt
      301 tagataaaca attttacact tttcattctt ttctctaagc atttcgccta gttcgatacc
      361 gctataatag gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat agatttcatt
      421 atttttaatg aattcaacaa attgtataac gttcaatccc tgaacgatct caaatttcca
      481 aggacttaat aaaattctgg attcttccaa aatttctact agcatttttt caaaaaccaa
      541 actatcatcc aaaataaata tacgcgttac cataaaaagt acctccactc gatattttac
      601 tatcaaaatc atttcataca acttttcaac atacatgtta ggaataaatt gtaattctct
      661 tcacaatctt ttccaacacc tttgaaaccg atatcaaata ctttcaggat gctgcttcta
      721 aaataaatat caaacaaatg aatacatcat gcaaaaaaag ccagacaacc gacgtccttg
      781 cgtgtcagct gtctgagcta tgtttattta ttttgttctt ttaaaaagaa cgatacacat
      841 actgattttt cggagcagag actttctcag cgtttgtaac aactacagat gggatttcac
      901 gcttgaatgg ttgataatag tctgattgga tggttccttc tattttatac cattcatcat
      961 ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc aaagactcct gaatctgcca
     1021 cacaatggat aatgccgaat cggaacaaga agatatctgt attgctatcg ttcggtgttt
     1081 ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa ttcgctaggg taattataaa
     1141 ttagctccat aacttctaaa tagttttcat ctgtgatctt gatagtttgc ttatccccat
     1201 acttttcttt caactcattc atttggtcta agtagtctga tttgttgaaa taaatactgg
     1261 tatccggctt taagtattgt gtctgcatct ccggatcccc tactgattct ttacttactg
     1321 ggaaattaaa tcctttggct tctacgatcg tagtgtcgag actaactgtt ggaaacaacg
     1381 tccccacgat tactggcaaa gctagcagta cataagcgat tccacgttga taaggttttg
     1441 acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc
     1501 acaaatacag ctgaactaaa gccaaaagag cagataaaat cattgaaaga atagctaagt
     1561 agcgataatg gacattgata tattgatcga gtttgccaga aacttgcaag tacatcatca
     1621 attcgaaata cccagataaa atcaaaaacc gcagcatatt ttccctccta aataaataat
     1681 gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta
     1741 aaatatcgtt tcatcatcag tagatttttg atatcgacca ttgggccaaa gaccaaaaag
     1801 gcagctacag gtgctgtgcc gaacagactt aacaaagaag atcctataaa cgcatctgct
     1861 tctgagcaaa gagacatcgt aaaggccaat aacagcatga tgataatggc taaaatcttt
     1921 gttgagccga gcgttagcat aattctagtt ggtaaataag tctgcatcgc agctgcgatt
     1981 aatccgccaa tgatcaaata acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac
     2041 acatgcccag tttgatggat cagctgattt tttggttcat tcttctcact ttgttcttct
     2101 tcatggacat gatgatggtg attttctgct actttagacg tgattttttc tttcaataca
     2161 gcgtcttttt gaaaataggc aagccagaca ccaatcacaa gggcaacaaa aaagctgcct
     2221 atcacccgcc agcttgccat ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc
     2281 acgattggat tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg aactggtacg
     2341 ccttttttaa caaattgatg aacgatcggt acgatgccgc attcacatga aggaaagaaa
     2401 aatcctaaga tcgaaccagt aaaaatagag aagatacgat tttcaggaag ccatctcttc
     2461 acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc taataatacg
     2521 aaaggtaatg cttcaataac gatcgataag aaaatcgtac tcatctgcaa aaccgaattt
     2581 ggaataaaag aaaacatgag tttcctcctt tttatttcta gttcattcgc tttgcaaata
     2641 tacgctttta ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact cttatttttt
     2701 accaaagcac ttcaattgac tttttctgtt attcccatta aattaaggtc tcatacatat
     2761 cacttctatc ataaaaggga gaataaaaat gaaacaaacc actaacttga ctgaagttca
     2821 agacatactg caacagtcat ccgttgccgt catttatctt tcgatgccta attgcagtgt
     2881 ttgtcacgct gttcgtccac gactagagga acttctcaca cattatgata ttccggcact
     2941 tcatttggat gcatttgaaa caccagaagt agctagtaaa tttgaagtgc tgactgcgcc
     3001 agttgtcctg atttttcatc aaggaaaaga agtctttcgg caagcacggt tcatcgactt
     3061 taaaaaaatc cgttatctac tagatgaact gacagcagaa gatgattccc tttcttatga
     3121 agaaatcttc cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa
     3181 agttttgcct tcatttgttc aatcgctttt tatttttatt ctgctgtatt ttcggaatgc
     3241 gcatttgcgt cagaatgcaa gaaatagatc aaggtttgca attcattcgt caaatcaaca
     3301 ttttggatca atacatctga cggagcattc aatcgagcag cggtaaagtt caagattcct
     3361 ttgattcctg cttccgttaa ctgattgact acttcttgtg ctctttcagc gggtatcgtt
     3421 aaaattgcta cttcgatttg ttggatcttg atttgttctg acatatcttc cattggataa
     3481 acggggatac cgtctacgat gcgcccaacg atttcctcat tcacatcaaa agcacaactg
     3541 atacgtatgc tattactttg atggaatttg tattttagta aagcacttcc caagttccct
     3601 acaccgatca gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc
     3661 ataagatctt ctacatcata accataccca cgttttccta attcgccaaa ataagaaaaa
     3721 tctcgacgga tcgttgcact atctacttga acggcttcac ttaattcagt tgaagacact
     3781 tttttctttc cagcattatt taaaatcctt aaatagcgat aatacaaagg cagtctttta
     3841 gctgtcgcct ttggaataat atgatctttc acgaaatgac ctccacgtat tgtctaatta
     3901 catctttgat ttctccatca tagcagtatt tataggaaaa aatcaattcc attgctcaga
     3961 tattgtgaaa ttttattctt tattcattta tttatcattt ctgcaaatct cgcagtataa
     4021 tggaatttat ggtagactac taattgaaaa atagtttttt atttgaggag aattcacaaa
     4081 gtagaaaaga ggattccatg atattattac aagccaatca gattgctcgc cattttggtg
     4141 cagaagtctt atttgaaaac atacatttag aaatcagtac aggcgctcgt gtcgctttag
     4201 ttggcagaaa tggcgctgga aaatcgactt tattaaagat cattgctgga attgaagcac
     4261 ctgatgaagg aaagatcgct aaaaataaaa cagcgactct cggatacctc gctcaggaca
     4321 caggacttga ttccaatgaa acagtttgga acgagatgct gaaagctttt gaacaagtta
     4381 gacaaatgga agagcggatg cgcgagctag agattttcat tagcgaaggt acgccagaca
     4441 cacctgctta tagttcttat ttaaaagaat acgaccggct gcaacacgat ttttcagatc
     4501 gaaacggcta tggttatgaa aatgaaattc gttccgtatt gcatgggttt aaattcgatt
     4561 cttcatttta tgatcagaaa atcgatactt tatcaggcgg acagcgcaca cgtttagctt
     4621 tagctcgcat gcttttgcaa caccctgaca ttttgattct agacgaacca acgaaccatt
     4681 tagatatcga gacactttcc tggcttgaga attatctgca aagctatact ggtgcattat
     4741 tgattgtttc ccatgaccgt tattttttag ataaagtagt caatgaagtc tatgaattaa
     4801 gcagaagaaa aatgcgccat tacaaaggta actattcgaa atatctagat ttgaaagcag
     4861 aacagttagc aagtgaatgg aaggcttatg aaaagcagca aacagaaatc cataaattag
     4921 aagacttcgt tgctcggaac cttgttcgcg cctctaccac aaaacgagcg caaagccgca
     4981 gaaagacatt agaaaaaatg gaccggctgg atcgtcctca aggggatgaa aaacaagcaa
     5041 actttctatt cagtatagaa aagccttctg gaaatgtagt cttgcaaatc gaagatgccg
     5101 ctatcggata tgataatgtc accttatctg aaccaatcaa tctagatatc cgtcgtcaag
     5161 aagcgattgc tctagtaggt ccgaatggta tcgggaaatc gacgttgctg aaatcaatca
     5221 ttcaccagat cccttttatc cgtggaaaag aaacgctagg gacaaatgtc accatcggct
     5281 attacgatca aggtcaagct gatctgcata caaacaagac gatcctagca gaattgtggg
     5341 atgagcatcc taccactcca gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt
     5401 ctggcgaaga tgtagaaaaa ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg
     5461 ctttagccaa gttagcgatg aacaaagaaa attttttgat tttggatgaa ccaacaaacc
     5521 atttagatat tgacaacaaa gaagtattag aaaatgcatt gatcgattat gacggcacac
     5581 tgctttttgt ttcccatgac cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac
     5641 tctctgaaaa tggcagcaaa gtctatctag gagactatga ttattattta gagaaaaagc
     5701 aggaagaaga agaaatcgcc gaattgttgg cacaagatga gccagaatct gctcctgctc
     5761 ccacgaacaa atctgattat taccaaagca aagaacagca aaagcttatc cgtagccttc
     5821 aacgaaaaat tactcaggtt gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt
     5881 tggaacaaga aatggtcgcg ccagaaaacc tggaagatca cgtaaaactc aatgagctga
     5941 accaagattt agaagctgct agacaagatc aagaaacaaa attgacggaa tgggaagaac
     6001 taagcttgga attagaagaa tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat
     6061 gctgttttgg tttttctttt tttattttat caaacaaaaa ttccatattt acctttttca
     6121 ccaataagaa gaccaattac tagtcattct cactaaagct tctaaataat agtaatcacc
     6181 ccaaatacaa ggtgtattta ccccattatt tttgggctta ttataaacag actgtgttaa
     6241 aaagccatct tcttcttccg ttgtagcata tctttctttc aattgtcgca taattttcca
     6301 aacagcagac tggtataagt tcttttctgt tttgtctaat agacctgatt ctattaattc
     6361 taataatcca caaaccatga tcaccatact agatgtatct ttttcttctc tgctagaatc
     6421 atcaaagatc aaatcccaat aacaaatggt atcttctggc aaccgattca taaaataatt
     6481 ggctaatatt ttgcttaaat caaaatattt ttgttctttc aaataacgat aacttaatgc
     6541 aaaaccgtaa atcccccatg cttggcctct agcccaacag gaattatcac tataaccttg
     6601 atgcgtactt ccatatcttg gttttccatc tttgacatcc atataaaatg tatgataagt
     6661 tgaaccatct tctcttacta aataatctgc tgcattttga atatgagcct gagcagcttt
     6721 tttatatttt tgatttccag tttcttcact cgcccaatac aataatggaa gattcatgca
     6781 acaatccatg atcattctac cttgttgtga aggatcacta ggatctcccc aagcttgtat
     6841 aactcctgcc ttctcccaaa aacgctgaag taatgtttct gcagcatcaa ttgaagcttc
     6901 cttcattttt cgatttcccg ttattttata atctgctacg gttgataaag aatacaaaaa
     6961 accaagatcg tgatgattag tgataatttg acagtcaatc ctgtggataa aatctatatc
     7021 tgctttttca gctgtttgtt gaaataaagg atcacccgtc ttctcaaata acaaccaatt
     7081 aattcctgtc caaaatcctt ctgtccaacc aatattgtct cttaatggat aacgtccttt
     7141 tataggacac tcgcttggga attgatactg actgttactg atacgtttga ttaacctctc
     7201 cgtgtgtctg cgacaacgct cgatctcatt attccattct ttttccatta attctttata
     7261 acattcattt atccattttt ctggatgtat tatctcttca aacatcactt tatcaagcat
     7321 ttccccacca gcctttagca taattttagt ctttttttat tgtttttatt ttggatagtt
     7381 agcccggttg tcccatcaaa atcaattttg ggacgattgt tccatacttc taaactataa
     7441 tcccgatctt gatgagcaac cactcctgtt ccgagccaat gggttccttt tccaaatgta
     7501 ccctctaatc caggaataaa agtgttttcc catgtcatca agtttgtatt gggcaaaccc
     7561 ataatagctg tggattttcg tttaatagta tcgtcactta aacagataat acctgaaaag
     7621 ccagcttcac tcaccctata gttttccttg tcttcttcta tgattattcc ttctttctca
     7681 ggagaacatt caatagcaaa ccctgtctca tatagaacat attctgtatc taattctact
     7741 ttgtgtacgc gaatctgcca gccttcaaaa ggaataagcc aagttctgat ttttgcctgt
     7801 aaagtaggtt cccatgtaga ggcaagtacc tctttcttac atacaatgtc tcgtacatta
     7861 tttcgaacga tgaactgtcc ataggttttg atgctctcat tcttaaaact ggtcgtgata
     7921 tatgtgtccg ctcgctgaat ccctagcatt gaatccattg ccatttctgc tttcaattga
     7981 ttcgctcgcg gtatcgaaaa accaaattga ctagaataag caaattttaa atatttttca
     8041 ttggtcaatg tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca
     8101 ttatcatgtt gtatgagcaa attggcttct gaaagaagtt tagttccttc acgaataggc
     8161 ataggcattt ctttagccgt ccaatactca tggtcttcag gtagcgccaa caataaaaaa
     8221 atcttattca gccataatgg agataacgta ctattatagg gttcagtcaa tgtcaattga
     8281 gggtaggtat aaccaatatt caggatacct ttttcatcaa atatttcttt ttgcatccac
     8341 catcttatat tccgattgac taaccccttg atttcaccta atttaaaagg caaaagatcg
     8401 gcccacaaca tagctgacca aaagcttgaa actgcaaaac gataaatcat gcttcgacca
     8461 aaaggcacat ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga aaaatcaata
     8521 gctcggttct tatatatctg gctaatgggc ttttctggtt ctagtacgct ataaatcaaa
     8581 ccataataat gaaatgcaaa aggattataa taatcttggc gacctcttga actgtctcga
     8641 taccagccat tttgaagata acaattttct atattatcaa atgcttcttt cagtgctgat
     8701 tctttaactg gataaccaaa tttttggata attgttccga caattatctt aaaaaattgc
     8761 caattaccat ctgcacattc atattcaaaa atttgcgaga accaatgaat aatctggctt
     8821 ttttctcctg tatctaattt tttccataat aattgttcat gatgatataa cgctaataac
     8881 attgcgggca tttctaccat acgctggtct ctttcatgta tttctcccca ataatcggga
     8941 tgggaaggat ctgttccagc ttttattcct gatagaatta atttcaacca tcgttcatct
     9001 aattggtcaa ttgcgggccc cgctccccat aaaacccgag agaatccttc cattcctgca
     9061 attttatttc catatcctac cccttgatgt atataatcaa tttgtgcgcc acttgctcga
     9121 taaaacggct ggagtggttg atttatcttt ttaaataagt caaagtaatc ctgtctggtc
     9181 cttaatagtg gtactggatg tttcattgta ttttctcctc tttttactac caaatagcta
     9241 attccgactg ttctaatcga agtaagcctt caataaaaaa ataatcagaa tagatcagtg
     9301 gcacattata agtcagttct ctatggtatg cttctgctcc gcctccaatg atcccatctt
     9361 catccatatt ccaatttgca tagtgctttt ctgtcttttg gagtatattg atagcagctt
     9421 catgatacat ttttgatcgt acagaggaaa catatcctgc taattccaga agaccgcatg
     9481 cagcgcatac tccagcactg gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag
     9541 gaatataacc agttttttcg attttctgaa taaaatttac tgctatcttt tctgctatag
     9601 ctagatagta cggatttttt gtgtaacgat aacttaacgc gaatccatat aaagcccatg
     9661 catttcctct gctccaacaa gaattctcat tgtatccttg cccgccaagt aaccgaatat
     9721 atttccctga ttctgcatca aattctgcga tatgcccggt tgatccatct ttcctcacta
     9781 gatacttagc tgttgtatct gcatgctgcc gagcaatttt agagaacctc ggatcaccag
     9841 tttccctcga agcccaatac agtaatggta aattcatcat actatcaata atcatccaac
     9901 caggtttatg gtcattccat gctactaaat agttccctga aatattatag cgtccagcaa
     9961 gtaccgtagc tgcatgtaac cctagttgtc gactatgttt gttttgagac tgttgataat
    10021 gtgcaatagc cgatggtaag aataaaaaac ctacatcatg atgaacgtct gaaaaatggt
    10081 acagggcaga ttctaataat tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt
    10141 ttgtcttgtg aaagagctgc cataaaatac cactccaaaa accattggtc caccagtcga
    10201 ttcctctgtc ctttattacg tctgtatatt ttccgttgta agaaatatat ggaattttat
    10261 tccccattct gtctaattcc gcatctatct tttcagaagc tacattcaaa atttttcttg
    10321 accattcctt cgtctgttca ttcatggata tcccgccatt cttttaattt ttatgaatgt
    10381 ggagttgctc ctagaacgaa ctagatggtt aacattcgtt atttttttta cttatttgca
    10441 gctccaatgt cattctcctt aaatttagac tgcgcccaca cataaatcaa gaccattggg
    10501 atcactgaag ttgctaaaat tagcataatt gcgccataat taattggctc acctgtacta
    10561 gaaaccgtca tatttttgat aattaaggga accgtatatt tatctttatc tgaaagcatg
    10621 actaatggaa atagatattg attccaactt cccataaaga taagtaaacc actagtaata
    10681 atcgtaggtg ataaaatcgg tacagcgatt ttcgtaaaaa tcacccactc attggcacca
    10741 tcgattctgg cagcctccat cagtgctgta ggaaattgac tgctatattg tcgcatcata
    10801 aacactgaag ttgtactggc aatcgaaggg atgatcacac ctgcataagt attcgataat
    10861 cctaaacgac tcataatgta aaataaagga atcaatgtgg taaaaaaagg caccatccta
    10921 gatagcataa ctgtattgaa caacagttta ttccccataa aacgatactt tgctaatgca
    10981 taacccgcca ttgtagaact ggctgtagct gaaactgtac tgattacagc aataacaatc
    11041 gaattccaaa taatcaaggg ataattatat ctcgccatga tggcttgcca gttttttaat
    11101 agcatatccc caaacttgaa attgattccg ccacttaaaa tttcttgatt actttgcgtt
    11161 gaagctacaa tcatatataa atatggcatc aaaaatatta aggcaccaaa cacaataatg
    11221 acgtacgtaa aaattctgct ttgtatcttt tgtttagtca tttttatcac ctccgaattt
    11281 aaattgaata gccgataata taacagatac aaacaccatc gtccaggcaa tagcagaggc
    11341 ttttccaaaa tcaaccattt caaaacttgt tttatataaa ttgactccta cgaccaaagc
    11401 tgaattttct ggtccattgg aagtcccaaa gaatatattt gggatattga aattacccaa
    11461 gccattgata gtggctaaaa ctgtggccat taaaatgatt ggctttagca tggggatcgt
    11521 taataaaaag aattgccgaa atttcgatgc tccatctatt tcagcacttt cgtatatctc
    11581 tttagaaatc ccttgaagac cagctaaaaa taaaatagtg ttatatccta cactaagcca
    11641 aatcatcgtg atgatcattg atattcgtat ccaaaaagga tttgttaacc actgtacacc
    11701 gttaattcct agcgcattca aaaatccatt gaataaccca gtcggattga aaaaagtcat
    11761 gaatacggaa gctacagcca ccgttgatgt aatggttgga atataataga cagttctaaa
    11821 aaatcctttt ccttttaact gtgctgaatt caataaaaca gcaaagtaaa tagctaatag
    11881 catcgttatt ggaattgatc ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa
    11941 caatggatct cggaagatag ctagataatt gtcaaatcca acaaattcaa atgagtagcc
    12001 aattttcata aaactaaaag caaacgagat aatgatggga atcaaaatcg tcacaataaa
    12061 gaaaataaca aaaggtgtga ccaatccata agcacttttt ttatgagacg tatctgttgc
    12121 ttttttcttg ctgttcttta ttgtcttgct tggtaacgaa gattgcatca tcgttaagtc
    12181 ctcctaatat tttttaatca ccagttaatt ttacgccgta cttatgagca aaattttctg
    12241 cttcttttag atatgaattt tcaaaatcac ctgtttgcca atagttatat gcagcggtag
    12301 tcaaataaga cgtagcatct gaataagtgg ctggtaaaac agtatttgat gtattttcat
    12361 tgattttatt caatagttca tagtattttt gtcctcctta gtagtcaaac tttttctcag
    12421 ctgcttctgt ttgataggcc aatgagttag atgtagccac gcccagctcc agtgcgttgg
    12481 ctaagcattc ttcatctgtc agcataaatg taagaaattg ttgtgccact tgtggatttt
    12541 tggcatcttt agggacataa aaacttgatc ctcctgatac tgcacctttt cctggatttt
    12601 cttttgaaaa aggaatcagt ggagccatgc gccatttgtc agatgcatca ggaaaattca
    12661 aagacatatt cgtagcatac cagccgccag caacaaacat tgcagattct tgaaatgctt
    12721 tctcagcatc ttgtgcaccg tagaaatgta caatatctga gtcgatcatt tctttgatta
    12781 actttgcagc atgtttcgct tctttagtat tcagacggat atttccttct tcatcaagca
    12841 aagattgatt ctgctgggct agcatgttga taaacaattc ttggtcccca gattctggca
    12901 aggcaattcc aaaactacca gttttttctt ttaatgcttt tgccacctgg atatattctt
    12961 cccaagaact gatttcttca aaatctacac ctatttcagc aaattttttt ggttgataat
    13021 aacaaagtgc tggggtaaaa tcactagccc aaggaataat cgtttcatta ctcgtttgtt
    13081 gacgcaaaat attcaatttt tgatcataaa actgatcgcc atatttgtca taaaatccat
    13141 attcatcagc actataaaaa gcatcttgaa atttttccag cacacctgct gctgaagtgt
    13201 ctgccatgga gataatatcc ggcatatctt cgtgtgttac caattttggg gtgatcattt
    13261 gtactgttgc tgttcccgaa gctacattca caatttttaa gtcaatattt gctccagttt
    13321 tgcggttata gatttttgca gcattttcaa catagtttgt cagtttgccg tttgcgacat
    13381 atacttctaa tgtagtggct ttttttatca ttggatctgc gatgtttgct gttacttctt
    13441 cgttagtttt tccacatgca cttaagctga ataatagggt aatgtttagc aaagctatca
    13501 ccaatttgta tctttttttc atgtctatcc cttctttctt ttcaagttct tatataaaac
    13561 gctttcactt atgtatatta acggaattag taacacaaat atttatataa aaaaatattt
    13621 tacttgtcgt tttggctttt catttttgtt ataatagtca tta
//
LOCUS       DAZKNM010000063        13106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000063 DAZKNM010000000
VERSION     DAZKNM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.064"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..1812
                     /gene="yfcC"
                     /locus_tag="ACG8M9_001492"
     CDS             73..1812
                     /gene="yfcC"
                     /locus_tag="ACG8M9_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0378273.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1969..3153
                     /gene="iadA"
                     /locus_tag="ACG8M9_001493"
     CDS             1969..3153
                     /gene="iadA"
                     /locus_tag="ACG8M9_001493"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0378274.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3264..3467
                     /locus_tag="ACG8M9_001494"
     CDS             3264..3467
                     /locus_tag="ACG8M9_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0378275.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3568..4230)
                     /locus_tag="ACG8M9_001495"
     CDS             complement(3568..4230)
                     /locus_tag="ACG8M9_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378276.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4243..5745)
                     /locus_tag="ACG8M9_001496"
     CDS             complement(4243..5745)
                     /locus_tag="ACG8M9_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378277.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6293..7972
                     /locus_tag="ACG8M9_001497"
     CDS             6293..7972
                     /locus_tag="ACG8M9_001497"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0378278.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8099..8578
                     /locus_tag="ACG8M9_001498"
     CDS             8099..8578
                     /locus_tag="ACG8M9_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0378279.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8797..9477
                     /locus_tag="ACG8M9_001499"
     CDS             8797..9477
                     /locus_tag="ACG8M9_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0378280.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9485..10816
                     /locus_tag="ACG8M9_001500"
     CDS             9485..10816
                     /locus_tag="ACG8M9_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0378281.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10831..11793
                     /locus_tag="ACG8M9_001501"
     CDS             10831..11793
                     /locus_tag="ACG8M9_001501"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0378282.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11904..12242
                     /locus_tag="ACG8M9_001502"
     CDS             11904..12242
                     /locus_tag="ACG8M9_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0378283.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12321..13028
                     /locus_tag="ACG8M9_001503"
     CDS             12321..13028
                     /locus_tag="ACG8M9_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0378284.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4162 a         2318 c         2877 g         3749 t
ORIGIN      
        1 tactgtataa ttcatttgat tatccatttt ttattcatca aaattactga aaaaagaaag
       61 gaagaaataa atttgaagaa aacaaattct tcaggaaaac cgaaacgcga cttcactaaa
      121 ttatcgacac cccatacgta tgtcattatt tttggtgtag tcatctttgc atggatactg
      181 acttttgtcg ttccagcagg aaagttcagc acgcaagata ttgaatacaa agatgcaaat
      241 ggcgaaacaa gtactcgtac tgttttacgt caagattcct tccgttatgc ttacgagctg
      301 gataagtcgt atgtgtttga tcaattagaa gaactgcaag atcatccagc agaacgtgaa
      361 aaactggatg ttccggaaaa aggattggaa aaagtcattg ctgacggaga aaaaaatctt
      421 acgcaggaaa aactagatga aatctccttg acagatgatg tgctttacga tcagtacggc
      481 gaaaatatct atgatacttc taaaaaattg cataaaacag ccaaaatatg gggaacagat
      541 gattttggcg gctttggttt cttaaatttt gtttttgaag gactagtatc tggagataag
      601 tatggatctg ctgtagggat cgctgcattg atccttgttg ttggaggtgc ttttggtatt
      661 attatgcgta ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa aactaaggga
      721 ctggagcgtt tagctttacc gctattattc tttgcttttt cattcggtgg cgctacattc
      781 gggatggcag aagaggtaat tccattctca atggtgatgg ttccatttgt cattgcgtta
      841 ggatatgact cgatcgtagc agtaaccgtg acttatgtcg catctcaagt cggaaatgcg
      901 acctcttgga tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg aatccccgtg
      961 ttgtctggtg cgacattccg attgattatg tgggtagttg ttactgcatt agccgctggt
     1021 tatatgatga tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt gacttacaaa
     1081 tcagatgatt atttccgttc tcatatcaaa aagacatctg atgaaaataa aaaattcatg
     1141 cttgggcata aattgatctt attagagatg ttagttgtcc ttgtctggat cgtttggggc
     1201 gtgactcaaa aaggctatta cattccagaa atcgcttctc aattctttgt gatgggatta
     1261 gttgctggaa tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa tgacatcgct
     1321 tcttctttcc aatctggtgc agctgattta gctggtacag caatcgtagt aggtatggcc
     1381 aaagggattc ttcttgtctt gggaggatct gatgcatctg ttccttcagc tttgaatacg
     1441 attttgcatg ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg tgcttgggcg
     1501 atgtacatct tccaaagttt gttcaacttg gttgttacat caaattcagg acaagcagct
     1561 ttgactatgc caatcatggc tccactggca gatttagtag gtgtatccag acaagtagca
     1621 gttcttgctt atcagcttgg ttcaggtttt atggacgctt ttacacccgt atcagccagc
     1681 ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag attccaaatc
     1741 aagatgcaag gattcctgtt tgttttagga actattttca tcatgattgc cattgcaata
     1801 ggtctgcagt agtactggag acctgtaaat aaaaaagtaa tccaaacagt agataaggac
     1861 tccaagaacc gtcattttac ggtaaaatca aaggagtaaa gatcctatcg tttggattat
     1921 tttttaagat cctatcatca gttatactta aagaaagagg aaaaatgtat gaaaatcatt
     1981 cgacaaattg aagtgtttgc accagattac ttaggaaaaa tggatgtact gattgccggt
     2041 gggaagattt tggctgtaga agaacagcta gaaggcggct acgaaggagt ggaagtagaa
     2101 gaactttcag gtgagggaaa agtactaacc ccgggtttca ttgactgtca tttccatatt
     2161 ttaggtggag gaggagaagg cggttatcaa aatcgaacgc cagaagtcac attgagccaa
     2221 ttgactacag ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg agaaggtaga
     2281 gatatgactg cgttgatcag caaagcgaaa ggtctggaag cagaaggaat ctccacttat
     2341 gtatacgaag gttcgtaccg attgccggta aaaacagtaa cagattctat catcaaagac
     2401 tttttgacaa ttgataaaat tatcggaatc ggagaaatcg cagtatccga tcaccgttct
     2461 tctcagccaa gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt aggaggcatg
     2521 ttatccggaa aagctgggat catcaatgtc cacttaggcg gaggaaaaag aaagctagag
     2581 ctattgacac gaatcgtaga agaaacagaa attccgatca cacaattttt gcctactcat
     2641 gcaaatcgca caccagaatt atttgaagct tgcgtggcat ttgccaaacg tggtggcacg
     2701 attgacttca ctgctagcga agatcctgat ttttgggaga agacagatgg tgaagttcgc
     2761 ttcagcaaag cactgaaacg attgatcgag gaagatgttt ctttagataa tttcacgatg
     2821 acctcagatg gacaaggaag cttgccttat tttgatgaaa acaatcattt cctagggtta
     2881 ggagtaggaa gtgccaaggc attgctagta gggatcaaag aagctgtgca aaaagaaagt
     2941 atccctttgg aaatcgcctt acgagctata accagtaatc cagcacgtat attgaaattg
     3001 gataaaaaag gaaaaatcga aattggtgct gatgcagatc tgtgtatctt ggataaagaa
     3061 acgcttgata ttgatacagt tatcgctaaa ggagaaataa tggtccaaga aaaagaagtc
     3121 aaagtatggg ggactttcga aaaaagtttt taatcaacag ctctaactat cttttttaga
     3181 aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt agatcaataa
     3241 acaaactaag aggaggaagc acaatggcag atttaaaagg acgttctaaa gatatgaaag
     3301 ataaagtagt cggtgaagga aaagaagcat acggcaaagc aacagacgac aagagcaaag
     3361 aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct cgaaatttag
     3421 gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa ccacttatta
     3481 ctaattgaat caagcaaata aaaaatccga accatatctg cgagacagat atggttcgga
     3541 ttttttattt gatcattatc ctgtttttta aacttcgatt ttttgaaatt ttttattttt
     3601 tagttcaaag acttcatcac agctttttgc tacttcaatc tcatgagtca cgatgataat
     3661 acagcgattt tgttcatggg cgatttcttg gaaaagtttg acgatatctt gtgaagttgt
     3721 ttcatctaga tttccagtag gctcatccgc tacgattagt tgatgttcac agcagatagc
     3781 acgaacgata gcgactcgct gctgctgacc tcccgaaagt ttggtcacct gtttttttgc
     3841 taatatttca tcaattccta cacgagccag tcgatctaaa gcaacttgtc gataattctc
     3901 ttgcttggtc ttagtgattt ccattgctgc tatgacgttg tctagagccg ataaataagg
     3961 aagcaagttg taagcttgga aaataatcga gacatcattt ttacgataac ttcttaaccc
     4021 gattttgttc agtgaacgat cttgatagta tacctttcct tcttttggtg catctagtcc
     4081 agaaataagc gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa tagcgtacat
     4141 ttttccagct tcgaaattca gttctacatc tttataaagc gcatcttgcg gatcttgata
     4201 ccaaaatccc aattgttctg tccgtaacat caaattcctc ctttagttta ataggatttt
     4261 tcttgggttt aatcttaata tccctataga agctaaaaag acagaaaaca gactgattcc
     4321 aaatgctact ccggctaata gtcccaattg tgcaggtttg acattgattt ccaattcgct
     4381 gacttgatca gaaacagcaa atggattatt cgtggttcct tgttgaccat cgcgttgacc
     4441 gccttgttct tgtctgttac ctggcatttg tccaccgcca ggagcttgct ggttttgtgt
     4501 cgtggtttct gtctgttggt caagtagctg ctgtcccacg gcattcgcga cgacgtttcc
     4561 gctaagcgaa gcaactccta atgcaagaat catacaaata gccacttcgg taaacaattg
     4621 gagaatgatt ttacttctgg attctcctaa agatagcaag acgcctaact catgccgacg
     4681 ctcccgaatg gtaatcataa tgatcagagt aagtatgata ataccggcta cagccactag
     4741 taaaacaaca tttttagaaa aacttgccac gttgtttaaa ggttccaaca ttgactgata
     4801 catggaatca tttgattgta agctaaatgt atctgtgtcg atcagcttct cagcttcttt
     4861 gagaaactca tccattttat ctggatcaga tagagtgtaa atagcagaat ctatcgtgtt
     4921 atcgtcagat gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa gtgtattcga
     4981 cggattcatg aagttgaatt tcatacccat actgccgctt gtttctctgg attcataaat
     5041 tcctttgatc gtcatttcat aagtggtatc ttcatcttca gtactcgtga ttgtgaaagt
     5101 atctccgact gccaggtcat ttgtttctgc tagagtagaa tcaatgacta cattgtttgt
     5161 gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt tttgctgttc catcagaaaa
     5221 attactattt tgtgcagttg ctgacacacc actgatttga aagtctgctt gagtcatttg
     5281 cggattcccg cccattcctc cagcaggttc accgcctgca tcagtagttt ctgaactgtc
     5341 ttcacttgaa tcagaactag tgatggcagt tattccatct tttgccatag ctgaggcgga
     5401 actttcaaag gaataactct ttacattatc taattcagct attttttccg cgtccgttaa
     5461 ggaaactggt gtaagagaaa aactgcctgg atctggacgt tcttcagtgg tagaaccgct
     5521 tgaagaagtt tgttcctgtt ttttaaatga agcttctcga ttggcactta aagtcactgt
     5581 tgctccaact tctttttgtg cttgattcgc tgcttcttct gcagcgcttc gaatcgtgag
     5641 gccagctagc acaaaaataa gaatggctgt gaacacggca aacaataaca atgaacgtcc
     5701 ccattttact tttgtacttt tccatgcacg tttcataaaa ttcatatttt gcatccctcc
     5761 tagtagatgc atttgataat agacaaccta tcttaaagct agcttaaatc ttatgctgtt
     5821 tgcacaccat caacatgagg ttctgtatat ataaaaagtc actatagtta ttgcaaaaaa
     5881 tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa gaggaatagc
     5941 aaaagaaatc ctagatgcta cagagggatt tttacgtgca tagttgttct aacaattgct
     6001 gatactagcg atttagttta tttgttgtat aataatgggg aacagctgaa tacttatata
     6061 agtattctgg ctatagcgtt tcgcaaaaga tccattttgt taatgaagta tatccaaaat
     6121 aaaatgccca tccaactgtc agaacgcgga tgtgctggta attttcctgc tgattctcca
     6181 taagttgaga aatagtgatt ttgcatatgc ttcattggaa tggctcatat atttagataa
     6241 agaagaaaat cgacatcttt aaacttggat gtcataagaa gaggtgaagt ttgtgagtac
     6301 cattaaaatt atcccattag gcggcgttcg tgaaaatgga aaaaacatgt atgtcgcaga
     6361 agtgggcgag gatattttta ttttagattg tgggctaaaa tatcctgaaa atgagttgtt
     6421 ggggatcgac gtcgtaatcc ctgattttac gtatttagaa gaaaacattg atcgtgtagc
     6481 tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg gcacttcctt acttgttgtc
     6541 aaaaatttct gtaccagtat tcgggacaga gttgacgatc gagttagcaa aattgaatgt
     6601 cagtcgtaat gaagcagcca aaggatttaa ggatttccat gtggtagatg agcatacaga
     6661 aatagacttt ggtgaaacag tagtgagctt tttccgtacc actcatacga taccagattc
     6721 agtggggatc aatctgaaaa caaaagaagg tagtatcgtt tatacgggag attttaaatt
     6781 tgaccaaacg gctatcccaa tgtatcaaac agattatgca cgattggcgg aaatcggcaa
     6841 agaaggagtc ttagccttac taagtgaatc ggcaaatgca gaaaatcctc agcctgtagc
     6901 ttctgaagcc caaatagcag atgaagtatt tgatacgatc aaatattggg aagggcgtat
     6961 catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg atgcagcaga
     7021 aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc gaacgaatta ttcgcacagc
     7081 gatgcgttta gaaaaacttc aattaccaag tgaagatctg cttgttacgt tgaaagacat
     7141 gaaaaactat gatccagaag aactgatcat tttagaaact ggccgaatgg gtgaacccat
     7201 caagtctctg caaaaaatgg caaacggtac acaccgtaat gtcaagattg aagaaggaga
     7261 tttggtttat atcactacta cgccaagcat cgcgatggaa accatcgtgg cgaaaacgga
     7321 agatatcatt tatcgagctg gtggaacagt gaagttgatc tctgagaata tgcgcgtttc
     7381 tggccatgcc aatcctaatg acttgcaatt aatgatcaat ttgatgaaac caacatattt
     7441 tgtgccagtt caaggagagt atcgtgaatt agctgctcat gcagatttgg ctcatgcagt
     7501 agggatgcca tacaaaaata tctatattac tggacgcgga gacgtgctgg aatataagaa
     7561 caagcgtatg agtgttgcag gaactacaag tgcagaaaat gtaatgattg atggattagg
     7621 cataggtgat atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag aagacgggat
     7681 tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa aacctcaaat
     7741 tacttcacgg ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag aaagcagcaa
     7801 tatgattgaa acaatcgttg agaaacattt agaaagcaat gattttgagt ggagcaaatt
     7861 aaagcaagac atccgagatc aattaggccg ttacttattt gaacaaacaa aacgccgccc
     7921 agtgatcttg ccagtcatta tggaagctac tcaacgtcga aataaaaaat aaacaaaaag
     7981 agtaagatga acctctctat agagtgctcg tcttgctctt tttttgcgaa aaacaaataa
     8041 tttttgaata cagcatccct cgctttataa taaacgatag aataaaggga gtgaatcaat
     8101 gatgagtcaa acaaaagtcg aacgttattt agaagaacaa aagatcagct atcaaccact
     8161 tgattttagt catgtggtag aagaatcttt cacagaagaa ctacaaaaaa gagggattga
     8221 tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg tgatcatcgc
     8281 attgattcct ttgaacgatc acttagatta caaaaaaacg agacaagtga cgaaagatcg
     8341 taaagttggt tttcctggaa tggattttgt attaatgcat acaggatatc cccacggagc
     8401 aaacacccct attggaataa aactcgcaca tcctgattat tgttttttag cagacagttc
     8461 tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa atcgggagaa gccttcggat
     8521 aaaagtagcc gatttagaaa agatcgtcca tcctatttac gctgatttaa ttagttaagg
     8581 gacgagaaga gaagctaagc agagcagaga cctcaaactt gagattatat ctgcttggta
     8641 cttggtgcat cctgtattat ctgttacaat tgctttgtac atactgtact atcaatccaa
     8701 tgaaaatata tttagcagct atattttttc attgatgtgc aagtgatgaa atcgttttta
     8761 ataaaaaaga tacagagaaa acgcgaggtg taaaatatga aaattacgta tcatggacat
     8821 tcctgtttaa caattgaaat ggaaaatggc caacgcgtga tgattgaccc gtttattaca
     8881 ggaaatccat tgacagactt aactgcagaa gaagtaaaga cagattggtt gctgattact
     8941 catggtcata gtgaccacat aggagatatg attcctattg caaaagaaaa tcaagccaca
     9001 attatcagta ttgttgagat tgctgattat gcgaacagca gaggagtaga ttcattcggt
     9061 atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt acatgcacag
     9121 catagttcaa gctatgaagt agacggtatc gttcagtata tgggcgagcc tagcggaatt
     9181 attattcaag cagaaggaaa aacgatttat catgcagggg atactgctta tttcagtgat
     9241 ctaggattgc ttgcagaaga attcgatatt gatgtcgctt tcttgccgat tggcgataac
     9301 tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc caagaaagta
     9361 gttccaatcc attacaatac tttccctgtc attgaacaag atcccgaatc ttttgtagag
     9421 ttactggaag atagtgaagg aaaaataatg agcgtctcgg aaacgctgga agtgtaggca
     9481 gaaaatggcg acaaaacatg atttgatttt agaatatatt gaaagtttac ctgttggcaa
     9541 tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa cagcgtaccg
     9601 tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca attcaacgag tagggaccat
     9661 ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg acatttggcg aggtcgtcag
     9721 gatcattgaa ggggacgttt taggcggggc ggcaggatta gacaaggtct tgaataaatt
     9781 cgtcatcggc gcgatgacta aacatgccat ggaacgctat attacgccag gatcactaat
     9841 gatcgtcggc aaccgggcgg aagtccaaaa attagcattg gaagatggcg ccgcagtatt
     9901 gatcacagga ggattcaata cgactgctga gattgcaaag ctagctgatg atcttgaaat
     9961 gccgatctta cgcacctctt atgatacatt tacagtcgca accatgatca accgagcgtt
    10021 aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt gatatctata tgccagctga
    10081 aaagactcat tatttgcagc agacagatac gatttctgat tatcagcgtt tgtcagaaga
    10141 aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg ggattgttac
    10201 ggcaaaagat gttttaggga aatcgccaaa ccagatcatc gatcgggtga tgacgaaaga
    10261 accaatcagt gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa tgatctggga
    10321 tgggctagaa gtcatgccgg tcgtttccga tgacttgaca ttggaaggct tgattacccg
    10381 gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg cagatacgat
    10441 ttcggatcag atatctggaa atatccatac gattgatact gaccgtgaag gaaatcgttt
    10501 ggaaaaccct aagttcaaat tcgtagtagc accacaaatg gtcaatggcg ttggaaccat
    10561 ttcttttggc gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa tggtgatgaa
    10621 ccaaaaacga aatatcttga ttgaacaagt gaacctacat tatttgcggc tgatccaatt
    10681 ggaaagcgaa ttagacattc gtccacgtgt attggaaatc ggcagacgtt ccgctaaact
    10741 ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg tttgtcaagt
    10801 aatggaacgt tcttagaaag caggagtgaa atgacagtac agcaagaaat agtagataca
    10861 ataaaaaaat acgataaaat cattatccac aggcatttgc gccctgatcc tgatgcatta
    10921 ggatcacaag taggattagc cgaactatta cgggaaagtt tcccagaaaa agaaatacgt
    10981 caagtaggtg aaacgattga agggcttcgc tttttagcag aaatgcaaga agttgaagat
    11041 gtcttctacc aaggagcgct agtcatcgtg actgatacag ctaattcacc cagaatcagt
    11101 gatcaaagat atcagttggc agaaaaactg atcaaaatcg accatcatcc caatgatgac
    11161 ccttatggag atcttgtgtg ggtaaataca aaagcaagta gctgtagtga gatgattgca
    11221 gaatttgctt tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc acggttactt
    11281 tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc cctccacgac gtcccgtaca
    11341 ttagagattg cttcgatatt gagaaattat ccatttgatg caagtgcttt gaacagagag
    11401 attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt atctttatca aaatatccag
    11461 gtggatgaaa atggtgcagg aagaataatc cttccgcaag aaattcttga taaatatgga
    11521 atcgatgatt cagagacagc ttcgatcgtt tcacttcctg gagtgattga cgaagtgctg
    11581 gcttggggga tttttgttca gcagccttca gggtattacc gcgtacgttt acgttctaag
    11641 gggccgatca tcaatgaatt ggctaaacgc catcatggag gaggccatcc attagcaagt
    11701 ggtgcgaatg caaaagattt agcagaagta gaggaaatct ataaagagat ccaagatata
    11761 tgccaatcgt ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg tgccttttgt
    11821 agtaaaaatc acaaatgtat cttttcaaga tcttcttttt ccgttacaat agtgctcact
    11881 aaagatttta aaggagaaag aagatgacat taccaaattg cccagaatgt ggctcagagt
    11941 acacatatga agatcgaggc ttgttgattt gcccagaatg cgggaacgaa tggaacaaag
    12001 aagaacaaga aactgtcata gaagacggat tagtggtatt agatgcaaat ggaaaccgtc
    12061 tgttagaggg cgatagcgtg acagtgatta aagacttgaa agtaaaaggt gcgtcaggtg
    12121 ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat cataatatcg
    12181 attgcaaggt agaaggattt ggaccgatga aactgaaatc ggaatttgtg aaaaagaatt
    12241 aaagaatatt caaggtcttt tcacattctt ttcctactac taatgataaa ataaagtaaa
    12301 cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta tggtttttta gatccgacat
    12361 ttcttttggt cattcttgga ttagttattt ccggaattgc gtcagcgtac gtcaatagta
    12421 ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt gactggtaca caagcagcac
    12481 aatatatatt acaaagtcaa cagataaata atgtcggtgt tcaacaaatt gccggagatc
    12541 tcacagataa ctacaattca ggtaataaga tgttgagttt gtctcaagca actgcacagt
    12601 ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg gtacaagatg
    12661 cttcgggtta tgtcccattg aagattcgtg cagcacttgt tccagcagct aactttggtt
    12721 cgatgatttc gttccccttg attatggtag gtgtattgct tagctggaac tctacgttaa
    12781 tcaatatcgg gattttcgct ttttctttag cattactttt ccaattggtg acattaccag
    12841 tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga ctactgacgg
    12901 aagaagaagt accgatggca agacacgtat tatttgcagc agcactgact tatgttgcag
    12961 cagcgctttc gacattcctg cagctgttgc gcttgatctt attgttcggc ggcaaccgaa
    13021 gagattaaaa taaagaagtt gtgaaatcag cgctttgatc tgagctaata ccgagagtta
    13081 gctgattgaa taccatttat ttggaa
//
LOCUS       DAZKNM010000064        12997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000064 DAZKNM010000000
VERSION     DAZKNM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.065"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG8M9_001504"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG8M9_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG8M9_001505"
     CDS             706..1734
                     /locus_tag="ACG8M9_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0378285.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG8M9_001506"
     CDS             1731..2489
                     /locus_tag="ACG8M9_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0378286.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG8M9_001507"
     CDS             complement(2575..3315)
                     /locus_tag="ACG8M9_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0378287.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG8M9_001508"
     CDS             3376..4629
                     /locus_tag="ACG8M9_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0378288.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG8M9_001509"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG8M9_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG8M9_001510"
     CDS             5300..5626
                     /locus_tag="ACG8M9_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0378289.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG8M9_001511"
     CDS             5694..6407
                     /locus_tag="ACG8M9_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0378290.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG8M9_001512"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG8M9_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG8M9_001513"
     CDS             7690..9555
                     /locus_tag="ACG8M9_001513"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0378291.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG8M9_001514"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG8M9_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG8M9_001515"
     CDS             11120..11821
                     /locus_tag="ACG8M9_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0378292.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG8M9_001516"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG8M9_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4355 a         2186 c         2749 g         3707 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt taaagaaaca tcatttatca gtgaaac
//
LOCUS       DAZKNM010000065        12889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000065 DAZKNM010000000
VERSION     DAZKNM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.066"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..2294
                     /gene="acm"
                     /locus_tag="ACG8M9_001517"
     CDS             129..2294
                     /gene="acm"
                     /locus_tag="ACG8M9_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0378293.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2566..3051)
                     /locus_tag="ACG8M9_001518"
     CDS             complement(2566..3051)
                     /locus_tag="ACG8M9_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0378294.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3141..3486
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3531..4172
                     /locus_tag="ACG8M9_001519"
     CDS             3531..4172
                     /locus_tag="ACG8M9_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0378295.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4191..4826
                     /locus_tag="ACG8M9_001520"
     CDS             4191..4826
                     /locus_tag="ACG8M9_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378296.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4844..5659
                     /locus_tag="ACG8M9_001521"
     CDS             4844..5659
                     /locus_tag="ACG8M9_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0378297.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5978..7414
                     /locus_tag="ACG8M9_001522"
     CDS             5978..7414
                     /locus_tag="ACG8M9_001522"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0378298.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7603..10050
                     /locus_tag="ACG8M9_001523"
     CDS             7603..10050
                     /locus_tag="ACG8M9_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0378299.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10280..11347
                     /gene="rlmN"
                     /locus_tag="ACG8M9_001524"
     CDS             10280..11347
                     /gene="rlmN"
                     /locus_tag="ACG8M9_001524"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0378300.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11337..11543
                     /locus_tag="ACG8M9_001525"
     CDS             11337..11543
                     /locus_tag="ACG8M9_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378301.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11540..12643)
                     /locus_tag="ACG8M9_001526"
     CDS             complement(11540..12643)
                     /locus_tag="ACG8M9_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0378302.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4403 a         2157 c         2741 g         3588 t
ORIGIN      
        1 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
       61 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
      121 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
      181 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
      241 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
      301 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
      361 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
      421 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
      481 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
      541 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
      601 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
      661 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
      721 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
      781 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
      841 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
      901 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
      961 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
     1021 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
     1081 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
     1141 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
     1201 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
     1261 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
     1321 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
     1381 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
     1441 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
     1501 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
     1561 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
     1621 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
     1681 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
     1741 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
     1801 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
     1861 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
     1921 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
     1981 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
     2041 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
     2101 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
     2161 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
     2221 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
     2281 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
     2341 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
     2401 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
     2461 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
     2521 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
     2581 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
     2641 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
     2701 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
     2761 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
     2821 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
     2881 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
     2941 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
     3001 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
     3061 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
     3121 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
     3181 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
     3241 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
     3301 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
     3361 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
     3421 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
     3481 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
     3541 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
     3601 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
     3661 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
     3721 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
     3781 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
     3841 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
     3901 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
     3961 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
     4021 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
     4081 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
     4141 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
     4201 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
     4261 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
     4321 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
     4381 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
     4441 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
     4501 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
     4561 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
     4621 ctattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
     4681 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
     4741 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
     4801 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
     4861 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
     4921 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
     4981 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
     5041 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
     5101 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
     5161 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
     5221 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
     5281 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
     5341 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
     5401 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
     5461 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
     5521 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
     5581 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
     5641 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
     5701 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
     5761 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
     5821 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
     5881 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
     5941 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
     6001 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
     6061 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
     6121 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
     6181 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
     6241 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
     6301 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
     6361 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
     6421 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
     6481 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
     6541 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
     6601 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
     6661 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
     6721 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
     6781 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
     6841 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
     6901 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
     6961 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
     7021 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
     7081 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
     7141 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
     7201 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
     7261 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
     7321 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
     7381 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
     7441 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
     7501 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
     7561 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
     7621 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
     7681 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
     7741 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
     7801 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
     7861 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
     7921 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
     7981 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
     8041 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
     8101 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
     8161 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
     8221 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
     8281 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
     8341 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
     8401 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
     8461 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
     8521 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
     8581 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
     8641 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
     8701 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
     8761 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
     8821 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
     8881 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
     8941 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
     9001 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
     9061 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
     9121 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
     9181 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
     9241 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
     9301 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
     9361 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
     9421 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
     9481 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
     9541 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
     9601 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
     9661 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
     9721 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
     9781 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
     9841 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
     9901 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
     9961 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
    10021 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
    10081 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
    10141 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
    10201 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
    10261 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
    10321 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
    10381 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
    10441 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
    10501 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
    10561 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
    10621 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
    10681 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
    10741 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
    10801 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
    10861 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
    10921 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
    10981 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
    11041 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
    11101 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
    11161 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
    11221 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
    11281 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
    11341 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
    11401 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
    11461 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
    11521 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
    11581 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
    11641 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
    11701 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
    11761 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
    11821 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
    11881 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
    11941 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
    12001 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
    12061 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
    12121 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
    12181 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
    12241 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
    12301 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    12361 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    12421 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    12481 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    12541 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    12601 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    12661 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    12721 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    12781 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
    12841 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattat
//
LOCUS       DAZKNM010000066        12821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000066 DAZKNM010000000
VERSION     DAZKNM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.067"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..3659
                     /locus_tag="ACG8M9_001527"
     CDS             132..3659
                     /locus_tag="ACG8M9_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0378303.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="ACG8M9_001528"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="ACG8M9_001528"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0378304.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="ACG8M9_001529"
     CDS             7447..7788
                     /locus_tag="ACG8M9_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0378305.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="ACG8M9_001530"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="ACG8M9_001530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0378306.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /gene="pheT"
                     /locus_tag="ACG8M9_001531"
     CDS             9145..11565
                     /gene="pheT"
                     /locus_tag="ACG8M9_001531"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0378307.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ACG8M9_001532"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ACG8M9_001532"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0378308.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4338 a         2237 c         2721 g         3525 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt c
//
LOCUS       DAZKNM010000067        12635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000067 DAZKNM010000000
VERSION     DAZKNM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.068"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACG8M9_001533"
     CDS             complement(146..1111)
                     /locus_tag="ACG8M9_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0378309.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACG8M9_001534"
     CDS             1345..2190
                     /locus_tag="ACG8M9_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0378310.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACG8M9_001535"
     CDS             2381..3568
                     /locus_tag="ACG8M9_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0378311.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACG8M9_001536"
     CDS             3574..3930
                     /locus_tag="ACG8M9_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0378312.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACG8M9_001537"
     CDS             3932..4270
                     /locus_tag="ACG8M9_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0378313.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACG8M9_001538"
     CDS             4665..5699
                     /locus_tag="ACG8M9_001538"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378314.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACG8M9_001539"
     CDS             5692..6378
                     /locus_tag="ACG8M9_001539"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0378315.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACG8M9_001540"
     CDS             6402..7250
                     /locus_tag="ACG8M9_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0378316.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACG8M9_001541"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACG8M9_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0378317.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACG8M9_001542"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACG8M9_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0378318.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACG8M9_001543"
     CDS             9516..10751
                     /locus_tag="ACG8M9_001543"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0378319.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACG8M9_001544"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACG8M9_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0378320.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACG8M9_001545"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACG8M9_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0378321.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4179 a         2366 c         2798 g         3292 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atata
//
LOCUS       DAZKNM010000068        12529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000068 DAZKNM010000000
VERSION     DAZKNM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.069"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..764
                     /locus_tag="ACG8M9_001546"
     CDS             27..764
                     /locus_tag="ACG8M9_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378322.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            778..1593
                     /locus_tag="ACG8M9_001547"
     CDS             778..1593
                     /locus_tag="ACG8M9_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0378323.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1606..2253
                     /locus_tag="ACG8M9_001548"
     CDS             1606..2253
                     /locus_tag="ACG8M9_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0378324.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2269..2925
                     /locus_tag="ACG8M9_001549"
     CDS             2269..2925
                     /locus_tag="ACG8M9_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0378325.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3087..3908
                     /gene="racE"
                     /locus_tag="ACG8M9_001550"
     CDS             3087..3908
                     /gene="racE"
                     /locus_tag="ACG8M9_001550"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0378326.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3911..5266
                     /gene="rph"
                     /locus_tag="ACG8M9_001551"
     CDS             3911..5266
                     /gene="rph"
                     /locus_tag="ACG8M9_001551"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0378327.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5267..5785
                     /locus_tag="ACG8M9_001552"
     CDS             5267..5785
                     /locus_tag="ACG8M9_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0378328.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5892..6392
                     /gene="cbpB"
                     /locus_tag="ACG8M9_001553"
     CDS             5892..6392
                     /gene="cbpB"
                     /locus_tag="ACG8M9_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0378329.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6672..7592)
                     /locus_tag="ACG8M9_001554"
     CDS             complement(6672..7592)
                     /locus_tag="ACG8M9_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0378330.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7718..8770
                     /locus_tag="ACG8M9_001555"
     CDS             7718..8770
                     /locus_tag="ACG8M9_001555"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0378331.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(8880..9635)
                     /gene="fabI"
                     /locus_tag="ACG8M9_001556"
     CDS             complement(8880..9635)
                     /gene="fabI"
                     /locus_tag="ACG8M9_001556"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0378332.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9855..10295
                     /gene="fabZ"
                     /locus_tag="ACG8M9_001557"
     CDS             9855..10295
                     /gene="fabZ"
                     /locus_tag="ACG8M9_001557"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0378333.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10410..11171)
                     /locus_tag="ACG8M9_001558"
     CDS             complement(10410..11171)
                     /locus_tag="ACG8M9_001558"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0378334.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11360..12454
                     /locus_tag="ACG8M9_001559"
     CDS             11360..12454
                     /locus_tag="ACG8M9_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0378335.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4036 a         2174 c         2642 g         3677 t
ORIGIN      
        1 ctaaataata atgggagtgt aattctatgg caatgattaa atttgatcac gtggataaat
       61 attatggcaa gttccacgca ttaaaaaata tcaatctcga attcgagaaa ggagaagttg
      121 tcgttgtcat tggtccttct ggttcaggaa agagcacgat gcttcgctgt atcaatggat
      181 tagagacgat ctcttcaggt aagctgctga ttaatgatac tgacttgcat gataaaaaga
      241 caaaactgac agaagtccga aaaaacatcg gcatggtttt ccaacatttc aatttgtacc
      301 caaataaaac tgttctggaa aacatcaaat tagcaccaat caaagtgttg aagcaagacc
      361 aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac tgtcaatatg ttagataaga
      421 aagattctta tccttccatg ttatccgggg gacaacagca acgggtggca atcgccagag
      481 gtttagcgat gaatcctgag atgctgctgt ttgatgaacc tacttctgct ttggatccgg
      541 aaatgattgg tgatgtatta gacgttatga aaaaattggc tcatgacggt atgtcaatga
      601 ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc ggatcgtgtt atcttcatgg
      661 ctgatggaga agtattggaa gacagcagaa atgtgagaga ctttttcgaa aatccaaatg
      721 aagtccgagc acagcaattt atcagtaaag ttatcaatca ttgataggag gaaaaagatg
      781 cgcaggttaa aaaaaatacg cctgattttc cttttaggca ttgcttttct tgcactagcc
      841 ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag agcgaagcaa atctaccaat
      901 gaaatcatct ggggtgtcaa gtatgatact cgattatttg gaatgatgga tattgagagc
      961 agaactgtac aaggatttga tgtagatatt gcaaaagcaa tcactaaaaa gatccttggt
     1021 gacaatggaa aaacagaatt tgttgaagtc acttctaaaa ctcggattcc attattgaaa
     1081 aacggaaata tcgatgcaat tattgcaacg atgacgatca cagatgaacg aaaaaaacag
     1141 gttgactttt cagatgtcta tttcgatgct ggccaagctt tgttggtaaa gaaaggaagc
     1201 caaatcaaaa gtgtagatga tttgaatgct tctacaacgg ttctagcagt caaaggttct
     1261 acatcggctg cgaatatccg acagcatgca ccagatgcaa aaattttaga attagaaaac
     1321 tatgcagaag catttactgc tttacaatct ggtcagggag acgcgatgac tacggataat
     1381 gcgattttgt tagggattgc agatgaaaat ccagagtatg agttagttgg tggaacgttt
     1441 actaatgagc cttacgggat tgcgatcaac aaagggcaag aaaacttctt gaaagctgtt
     1501 aaccaagcgc tagaggaaat gcatgcagat ggcacatacg ataaaatcta tcaaaaatgg
     1561 tttccaaatg aaactgaagg aaaagtagag taagggggag aggcaatgag cgaattgata
     1621 caaacttatg gaccaatctt tgtcgacggg ttcaagatca cattgttttc gagtgtctta
     1681 gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa tcttccaatt atcaaagaat
     1741 aaagtagtga gcgccttagc aaaggcatat gtagaatttt ttagaaacat tccattatta
     1801 atcatcgtga tgttttttta tgttgtcgta ccgatttatt ggtttaatct agatggattc
     1861 caagcaggta cgattggatt aacgatctat acatctgctt ttattgctga aacagtacgt
     1921 tcagggattc aaaccgtacc aaaaggtcaa acagaagcag gactatcttc tggttttacc
     1981 tattcagaaa tcatgcgtta tattgtttta ccgcaagcat tcaaaatcgt tatcccgcca
     2041 ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta ttttagcaat cgtagctggt
     2101 ttagatttga tgtatcaagg agacttgatc gctagtgaaa cattcaatac cttcgatacg
     2161 tatatcatcg ttgggttatt ttacttagtt atcacgctac cgttatctta cttaatggtt
     2221 tatcttgaga aaaaatgggc ggttcgttca tagaaggagg ggaaatacat ggattttagc
     2281 ggcgcttttt catggatgaa tatccgtttt ttattggaag gattaaaggt tactgttgaa
     2341 gtttcattgt tttcgatcat ttttagtttc ttgatcggtg gtctgacagg tgtgattcgt
     2401 tttgcgaata tccctttctt ttccaaaatc cttggtgtag tgattgatat tatacggaat
     2461 cttccattat tattgatcat tttcttcact tactttgcct tacctcaaat cggcattcag
     2521 atgaacatct tctggtccgc tgtcgcagca ttaacgattt ttgagtcagc tatgttgtcg
     2581 gaaattttcc gagctggact caatgctgtt ccaaaaggac agatggaagc aggattatca
     2641 acgggactga cttatgtaga atcgatgcga acgatcgtgt taccgcaagc atttaaatca
     2701 atgatcccgg caattgtgag ccaattgatt tcactgatca aagatacttc attagcagta
     2761 atcatttctt tacctgaact ttctcatcag gcacgtatta tttatggaca gaacaccaat
     2821 tatgtgcttc cgatgttcat catcatgaca cttatgtact tcgtggtatg ttacgcgctt
     2881 tctctgcttt ccggttactt ggaaaagaga cggtacagtt attaatagat gattctttta
     2941 gcggttgtgg caaactaaag tcacaaccgc ttttcctctt tccattcgaa aaaaagagat
     3001 ttcgattgtt ttaagcaagt aagagcactt gaaaattcac gctaaaaagt acacaatagg
     3061 gaggatagaa tttattgaaa aaaaggatga tacgattgac agataatcgc cctatcggat
     3121 ttattgattc aggtgtcggc ggcttgactg tagtaaaaga agccctgaaa caattaccga
     3181 atgaaaatat tttatttgta ggagacacag cacgctgccc atatggccct agacccgcgg
     3241 aacaggtaat ccagtatact tgggaaatga cggattatct ggtggagcaa ggaatcaaga
     3301 tgctggtgat cgcctgcaat accgcaactg cggtggcttt agaagaaatc aaagctgctc
     3361 tttctattcc agtcatcggt gtgatccttc ccggtactag agcggcagta aaaaaaacac
     3421 aaaataaaca agttggcatt atcggtacga ttggtacggt aaaaagtcaa gcttatgaaa
     3481 aagcactgaa agagaaagta ccagaattga ctgtgacaag tcttgcttgt cccaaatttg
     3541 tttcagttgt cgaaagtaat gaataccatt catcggtggc gaaaaaaatt gtggcagaaa
     3601 cattagctcc tttaaccact aaaaaaatcg atacattgat tttgggatgc acccattatc
     3661 cattattacg ccccatcatt caaaatgtaa tgggagaaaa tgttcaactg atcgattctg
     3721 gagcagaaac agtaggtgaa gtatctatgc tgttagatta tttcaatttg agcaattcac
     3781 cgcaaaatgg tcggacatta tgccagtttt atacaactgg ctctgccaaa cttttcgagg
     3841 aaatagctga agactggctt ggaatcggac acttaaatgt agaacatatc gaattgggag
     3901 gaaaataagg atgcgtcatg acgggagaaa agcccaagag cttcgcaaac tggagcttca
     3961 aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc cgttttggtg atacaacagt
     4021 tatttgttct gcaacaatag aaaattctgt tccccctttt ttgagagaaa caggaacagg
     4081 atgggtgaca gcagaatata gtatgctgcc tcgagcaacg agtacgagaa atcgtagaga
     4141 aagcagcaaa gggaaacttt ctggtagaac aatggaaatc cagcgtctta ttggtcgttc
     4201 cttgcgtgca gtagtagact tggaaaaatt aggagaacgt agtatcatcg tagactgcga
     4261 tgtgatccaa gcggatggcg gtactagaac agcaagtatc actggggcat ttgtggcatt
     4321 gaaattagcc attgaaaaac ttttaagaga aaaagaattg tcagaagatc caataaaaga
     4381 acatcttgct gcagttagcg taggaatctt accagatggg acttgcgtca cagatttgga
     4441 ttaccaagaa gattccgcag cacttgtaga catgaatctt gtaatgacag aatctggaaa
     4501 attcgtggag attcaaggaa ctggagaaga agcaacgttt gatggtgaac agttgaatga
     4561 aatgctcttt tttggaaaaa acgcgattga agatttgatc aaagagcaaa aacatgcttt
     4621 gctaacagag tttgctcaaa atgatgagcg gatagaagaa acaaaaacaa tcatcattgc
     4681 gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg ttcaaagaag cgggatatca
     4741 tgtaaaaaca ttacttgact atcctgaatt gccagatgtg gaagaaactg gcagcacatt
     4801 tgaagaaaat gcccgattaa aagcagaaac gattgcacaa ttgcttgatc agccagtctt
     4861 agcagatgat tcaggtttga aagtggatgc tttaggcggt atgccaggaa tctattctgc
     4921 acgttttgca ggggaacaaa aaagtgatgc ggggaataac gccaaattat tatatgaatt
     4981 gacagatgtg ccggatgaaa aaagaacagc acaattccat tgtacgttag tattcgcggc
     5041 acccaaaaaa gacagccttg tagtagaagc ggaatggccc ggtcgagtag cccgtatacc
     5101 atctggcgaa aatggctttg ggtatgatcc tttatttatc ccagaaggta agaaacaaac
     5161 agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt catcgggctc aagcaatgaa
     5221 aaaattgagt gccgagtgga aacaatggct ggaaggagag agatagatgc ggtatttagt
     5281 agtaagtgat aatcatggag acagagatat cctcgttgac ttagtagatc gttatcgttc
     5341 tgaagtggat atctttttcc attgtggtga ttcggaatta gaagcttctg acccattatg
     5401 ggatacattc caagtggttc aaggtaattg tgattatgga ccaggattcg aacagaaaaa
     5461 agtgatccaa actggtcagg atacagtatt catgactcac ggtcatttat cgaatgtccg
     5521 atttggtttg acacagttag caatcgaagc tcgagctgca actgccgata tggtgttttt
     5581 tggtcatacc catcaaatag gctgtgagat ggatcagaaa atcctgtttt tgaatcctgg
     5641 tagcatttct cagcctagag gcccgatcca aatcccatca tatgctctta tcgaaagttt
     5701 agatgaccaa atttctgttc aatactataa ccgtgcccat caggcagttg aagagttggc
     5761 cttttctttt tcgagagagc gctgaataaa aaaactctaa tttttaataa aaataagctt
     5821 tattttatga attttgcatc cggtttcggt aaaatagtag tgttgaatga aatagaaatg
     5881 gaggccatca gatgattgga cctatagtaa gagaaatgct gctggaaaat caggaaacgt
     5941 ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca tcatccattg tcccatggtt
     6001 tacttgtgtt atcgaaggtc ggttattcca aaattcctgt attaggaaaa gatgatcact
     6061 ttgtaggact agtaagttta tcagatgtgg tcaataaaat gattgacttg caaacaatca
     6121 gcatggaccc gttagaagga ctaacggttg ctgatgtgac agaaacagat gttccgacaa
     6181 ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt agtcgattcg ccttttttac
     6241 cggttgtcac agaagatcgg attttcaaag gaatcattac aagaaaagag ctgcttaaag
     6301 ccgtaaactt catggtgcat gaattagaac gtcgtaatat attgtctccg aaaatcgaag
     6361 aattaaaaga acaaatcgat atcgtgagct aaagaacgag aaactaaaag ataatcaaaa
     6421 aataagtaga aaaaacagcg taccagtttt gactttatct tgaaaaagag gctgggacaa
     6481 aagcgtttaa ctccaagaaa taagaaggaa ttcacgaaaa ttgcttttca aatttttgtg
     6541 aatttcagct tatttccgaa ggagttgctt ctgcttccgc cgtttatacg tgtttagagc
     6601 gtgggacaaa agtgttcttt acttttgtcc cacgctcttt ttagtgagat tcgttgcatc
     6661 gtttgtccaa attattttcc gacagcaatt ggactgttcg gaatcgtgaa accagaagct
     6721 gtcaattctt tgacatagga gctcaagaac tcttctttta aggcatattg ttttccgttc
     6781 aatgcgtagc agaccgtacg aatcgcaaaa ttgctgtttc caagatccac taatccaaag
     6841 atagaaggtt cagtttggag ctcttcttgg tatttttcag ctaatgtacg atttactcga
     6901 tcaatgatct catagatttt gtcatatcct tcttcaggaa caatccgaat atctaaaagt
     6961 acttgcatat ttgcacgaga gagattgctg atcgtggtga tgtttcgatt cgggatgaaa
     7021 tggacagtac catccgttgc tttcagctga agggtacgga taccaacaga tacgacggtt
     7081 ccttcaatcg ttaagtttgc tagcttgatg tagtccccaa catccatctg ttgttccatg
     7141 ataataaaaa atccggtaat aacatcactc atgaaccctt gggcaccaag accgattgcc
     7201 acacctgcaa ttccggcacc agccaaaagt gatccgatcg gcacaccgat agcagataaa
     7261 attgcataga taaaaaagaa accaagggta taatgaaaaa tagtatttaa aagactatgt
     7321 aaagtcttta agcggctctc gtttagaatg gcttttttag aatactgatt aaaagactta
     7381 tcaatcagat attttcccac acggtgaaga atccaaaaaa ggacgatcaa aaaaatcaga
     7441 tataaacttt tttggataaa ggcagccaaa atggcgtccc aatcaatccc gtcccaaaac
     7501 cgctgaaacg catttacttt gctgacagct tgatctgtca atgtctcgcc agaagagtct
     7561 gtcgtaccgg cagttgctaa taatgaaaac ataaaatcct cttttctaaa atttgattct
     7621 ttctgtattg taccaagaag ttttcaaaaa aggaaatagc aaaaataact ataatgaagg
     7681 aaaaaagctg aaaataaaat gacaaataat atggataatg ataaaatgtg gagaaaataa
     7741 cgaaggtggt aagtatatgc aaatcgattg gaacaatcta ggtttttctt atatcaaaac
     7801 tccttggcgt tttattgcca aatggaaaga cggacaatgg gaagaaggag aattgacaga
     7861 agataattac ctgacgattc atgaaggctc tccagcgctt cattatggtc agcaatgttt
     7921 tgaaggtcta aaagcttatc gtcgaaaaga cggcaaggtc aatctctttc gacctgaaca
     7981 aaatagtcga cggatgaatc aaagtgctag acgattgctg atgccaatgg tccctgaaaa
     8041 gatgttcgtt gatgctgtca agcaagtggt aaaagcaaat gaagattttg tgccgcccta
     8101 tggaacaggc gctacattat acttgcgccc tttactgatt ggggtcggtg aaaacattgg
     8161 tgtccatcca gctccagaat atttatttgt cgtcttctgt acgccagtag gagcgtattt
     8221 caaaggtgga ctaaaaccaa cgaattttat tgtatctgat tacgatcgtg ctgctcctaa
     8281 ggggactggt gcagcaaaag tcggcggaaa ttacgcagcg agtttacttc caggaacaga
     8341 agcaaaagaa ctggattact cagattgtgt ttatcttgat ccagccacac atacgaaaat
     8401 cgaagaagta ggtgcagcaa atttctttgg aattacaaaa gacggaaaat tcatcactcc
     8461 gcaatcgccc tctatcttac caagtatcac gaaatattcc ttgttgcagt tagctgaaga
     8521 acgactagga ctgcaagcaa ttgaaggaga tattttcatt gatgatctag atcaatttgc
     8581 agaagctggt gcatgtggta cagcggctgt tatttctcca atcggtggga tttatcacaa
     8641 taacaatctt catgtatttt atagtgaaac ggaagtagga ccagtaacaa aacggttgta
     8701 tgatgaattg acaggtattc aatttggaga catagaagca ccagaaggct ggattgaagt
     8761 cgtggaataa tttcaaagta atgagttgaa aaaaacgtaa aaagaaaaaa tcaggaagag
     8821 actttaaaaa gttttttcct gatttttata tgttttactt taaagaatgc aacaaaagtt
     8881 tatgaaagat ggacaccttt gtcaacgtag ataatatcac cgatgattcc gctggaaagt
     8941 gggctgatta agaaagcaca agcattaccg acttcttcga tcgtcacacc ttgctgatca
     9001 ggcgtacgag attcagataa ctggatcagt ttttgatagt ctttaacacc tgtgacagct
     9061 aatgttttga ttgccccagc tgagattcca ttgacccgaa ttcctttagg cgagaactca
     9121 gctgccaaat aacggatagc agcttcaaga gaagctttcg caatacccat catgttataa
     9181 ttcgggattg cgcgttctga acctagataa gtcatgctaa cgatcccgct gtttggtgca
     9241 agaaacggtt gtgcgtattt tgatacagca attaaagagt agctgctgat atcttgagct
     9301 aattgatagc cgtctctgga aatatcagac acatttccgg acaattcttc tttgttggcg
     9361 taagcaactg catggacaag gccgtggatc tccccagcat attcaccgat ggcattcatt
     9421 gctttttcga tgctttcgtc gctagaaaca tcacattcta cagtgaatgc ttcttcgccg
     9481 accaatttga ctaatgattt tttcattcgt tcattttggt aagtatagat aatttctgcc
     9541 ccttgtttca acattgcttc agcacaaccc caagcaatgc tttttttgtt agcgacgccc
     9601 ataatgacga ttttttttcc ttctagaaat gacataatga ctctcctttg tttctgataa
     9661 aaatttgata gtcgtaaaaa aatagagaat aatagctaaa ataacttctt gatttcttaa
     9721 ttattagtca aaatgttttg attgtcaaac tattttttct taaattaaaa aaagattgct
     9781 aaatatttag gaatatgata ctttgatagt caaagtaatg aaaatggcaa ttgcctgaaa
     9841 gaaggagttt aattatgtct tacgtattat ctgctactga agtaatggaa ttgattccta
     9901 atcgttatcc tatttgttat attgattatg tagatacttt agaaccagga aaaaagatcg
     9961 ttgcgacaaa aaatgtaaca atcaatgaag attttttcca aggtcacttt cctgacaatc
    10021 cagtgatgcc gggtgcgttg attctagaaa cactggcaca agcaggttcg atacttatcc
    10081 taaaatcaga agagtttcta ggaaaaagag cttatatcgg tgggatcaat aaggccaaat
    10141 tccgtcaaaa agttgttcca ggagatgtgc tgaaatgtca ttttgaaatc accaaaatga
    10201 agggatcagt cggtacagcg ttatctgcag catatgtaga tgataaaaaa gtctgtgagt
    10261 gcgagttcac gttcatcgtc aaccaaaatg cttgactata aaacctgata tagcaaaaca
    10321 aaaggaagct tacttgcttt ttatgtaaaa agaagacgcc tgaatcctag catacgttgt
    10381 catctatgca ggattcagac gttatttttt tatttttgtt ttgcttcttg cagtatattg
    10441 attttatcga tttttacttc ttcttttggt ttgtctgaac tatctgtttc gatatttgca
    10501 atagcatcca cgacatccat tccttcaatc acttggccga agacagtata atggccatct
    10561 agtgaaggat aaccgccgtt tttataagcg tcgatgattg cttgaggata gtcgtctttt
    10621 aaaagaccat catgcatatc atctgtattt tgaacgataa agaactggct gccgttactg
    10681 ttaggatctt gtgagcgagc catagataac gcacctctta tattataaag gtggttgttg
    10741 atctctgttt caaatccttc tccccaaatg ctttcccctc cagtgccatc gcctttagga
    10801 tcgccacctt ggatcatgaa atctttgatg acgcgatgga aagtcagtcc gttgtagtaa
    10861 ccgtcttttg catgtgtcat aaagttttca accgcttttg gtgcgtgttc gggaaataat
    10921 ttgattttga ttgtgccttt attcgtcacc atttcgacca gatcttcatt ttcggcgaca
    10981 tctttcgtca attgtggtaa ttccaatgcg ttcaaatcga cgctactggc agtcgtgctt
    11041 tccgcagtag atgtactttt tgaactttct gtagacgaac tagaagaagt cgtgttgttt
    11101 gcagaacaac cagcagcaaa cagcaatgct gctgctaaac taatacttaa aataacttta
    11161 tttttcttca taactgttcc tttccaattt actattttgt tatctttaat gataacaaaa
    11221 tagagggaaa atacctactt aaattattgg aatttatcat ttattttaaa agaaaaagaa
    11281 aaggtgtaga ccatttactt tgtgtttgct ataatgtaag tggatacaaa aacgaaaaga
    11341 tgcaggagga aatggtaata tgggaaaatt aggaatctcg atttatccag aacggtcaac
    11401 atttgaaaag gacaaagcgt acttggattt agctcataag tatgggtata aacgggtttt
    11461 taccagtttg ctgcagatca atgatgataa agaaaaagtt ctttcagaat ttaaagaagt
    11521 agttgattac gcgaatagcc taggcatgga agtaatggtt gatatcaatc cagcgttatt
    11581 tgaacagttg gagatttctt acgatgactt atcttttttc cataagatgg gtgcctacgg
    11641 ggtccgctta gatatcggat tcacaggagc agaagaagca aaaatgacgc gcaatccttt
    11701 tggtatcaaa atcgagatca atatgagttc gggcacgaat tacgttgaca acatcatgag
    11761 ttattcgcca aacacggata atttattagg ttctcataat ttttatcctc atcgatactc
    11821 tggtttagga tacgaacact ttgttttttg ttcagaaaaa ttcagaaaat ataatttgaa
    11881 tacgatggca ttcgtcaatt cgcaatctgc tgagtttggc ccttggccta cgcaagatgg
    11941 cttgtgcacg ctggaagacc accgagatct tgaaattgcg acacaagtga agcatttaat
    12001 ccttacagga ttgattgatg atatttctat tggaaatgcg tacgcaagtg aagaagaatt
    12061 gaaagaaatg gcagaagcat ttaacgcgga ttatccaaca ttgaaagtag atacggaaga
    12121 aggtattaca gaaaacgaac gaatctgctt gtttgataac cttcatagtt atcgagggga
    12181 tcgctcagaa tatatccttc gttcaacgat gactcgtgta tactacaaag ataaagattt
    12241 cccgcctcat aatacgcgag atatgcatca tggggatgta ctgattgaca atgaagggta
    12301 cggtcaatac aaaggagaaa cacaaatcgc cttgaaggat atgaaaaacg acggacgtgt
    12361 caatgttgta ggaagaatct ccgatgacga attattcctc cttgactttt tgaaaccatg
    12421 gtcaagcttt aaattaattg aaaataaaaa atagataatt agttattgca gaaataatcc
    12481 gctagaaata gccagttatt tctgcttttt tattttgttt ttttatagg
//
LOCUS       DAZKNM010000069        12411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000069 DAZKNM010000000
VERSION     DAZKNM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.070"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..3211
                     /gene="fss3"
                     /locus_tag="ACG8M9_001560"
     CDS             23..3211
                     /gene="fss3"
                     /locus_tag="ACG8M9_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0378336.1"
                     /translation="MLSLAVQAIVGAIAPTITFADEITHPQTVTVELDLAHQYAVEGT
                     FSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKNPLPDMSDKAKLVS
                     VLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAIEAKINQAIDDYQK
                     KPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVNGNQLIITPNANSK
                     ESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVETEGSLKIKKVDKE
                     SGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTT
                     PMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSLAGNTFAIRKDSPT
                     GEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELKYANQT
                     VAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTLFTAKDGKAVKWSE
                     AFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAPEGYSLDETKYPVS
                     IKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGFNDLTFKVSPLEGT
                     NEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRSTFK
                     ENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNRLMLYDLPEEEDSL
                     TSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDVDATKAAQEKDEKA
                     KPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYENKEAFEAGDKPVA
                     KDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFDDVSITHDVLDGSK
                     EAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDTGKYPEGTKFTFAE
                     INYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVPSGSQESSPTVKTF
                     PQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3324..3638
                     /locus_tag="ACG8M9_001561"
     CDS             3324..3638
                     /locus_tag="ACG8M9_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0378337.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3651..4025
                     /locus_tag="ACG8M9_001562"
     CDS             3651..4025
                     /locus_tag="ACG8M9_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0378338.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4035..4370
                     /locus_tag="ACG8M9_001563"
     CDS             4035..4370
                     /locus_tag="ACG8M9_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0378339.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4453..5793
                     /locus_tag="ACG8M9_001564"
     CDS             4453..5793
                     /locus_tag="ACG8M9_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0378340.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5871..6545
                     /locus_tag="ACG8M9_001565"
     CDS             5871..6545
                     /locus_tag="ACG8M9_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0378341.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6538..6702
                     /locus_tag="ACG8M9_001566"
     CDS             6538..6702
                     /locus_tag="ACG8M9_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378342.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6778..7920
                     /gene="dcm"
                     /locus_tag="ACG8M9_001567"
                     /pseudo
     CDS             6778..7920
                     /gene="dcm"
                     /locus_tag="ACG8M9_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            7910..8200
                     /locus_tag="ACG8M9_001568"
     CDS             7910..8200
                     /locus_tag="ACG8M9_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378343.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8459..9643
                     /gene="mobT"
                     /locus_tag="ACG8M9_001569"
     CDS             8459..9643
                     /gene="mobT"
                     /locus_tag="ACG8M9_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0378344.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9640..9777
                     /locus_tag="ACG8M9_001570"
     CDS             9640..9777
                     /locus_tag="ACG8M9_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0378345.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            10523..>12411
                     /gene="ltrA"
                     /locus_tag="ACG8M9_001571"
     CDS             10523..>12411
                     /gene="ltrA"
                     /locus_tag="ACG8M9_001571"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0378346.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAG"
BASE COUNT         4257 a         2065 c         2763 g         3326 t
ORIGIN      
        1 aaagaaatta tgtgtattaa ctattttgag tttggctgtt caagcaattg ttggagcaat
       61 tgctcctacg attacttttg ctgatgaaat tacccatcca caaacggtaa ccgtagaact
      121 agatttagca catcaatatg ctgttgaagg aacttttagt gatggtcgtc caatgtcaga
      181 agtcactgtt ccgcattatg cagtgtacaa cggctttaaa caagatattt tctgtattga
      241 accaggagtc ccgatttaca atgagttcac tcctggctat gagaaaaacc cgttaccaga
      301 tatgtccgat aaagccaaat tagtttccgt tttatggaaa aaagtaggta cagatgcaga
      361 tactcatatt gtggcacaga agatgatctg gcaagaagtt aatgggtata cccttcactc
      421 aataaaacga tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa aaataaatca
      481 agccattgac gattatcaga agaaaccaac cttccataat agtacggcta aaacagtatt
      541 agggcaatcg actactgtga cggatacgaa taatctgaat ttgtcagagt tcgatgaggt
      601 tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt aatcagttga taatcacccc
      661 aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag aaatctgctg gtaccggaac
      721 acctgtcgcc tataaaaagg ctggccaaca gactttaatg gccggggcga ttgataaacc
      781 taatacctac acaatcaaaa ttgatgtgga aacggagggt tctttaaaga tcaaaaaagt
      841 cgataaagaa tcgggtgatg ttgtaccagg aacggttttc catttagact ttggaaaaac
      901 tttacctgca aaagacgtga ctactgacaa agaaggcatt gctacattgg atgagattcc
      961 tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg gcgccttata cgattgatac
     1021 cacccccatg actaccacca ttaaagcagg cgaaaccatt tacgtgactt caaaaaacgc
     1081 acgggaaaaa gggcaaatca ttctggataa aagcggagtt gaaacaggga gtgatctttg
     1141 gaatgacaat tattccttag ctggtaatac gtttgccatt cgcaaagaca gtccaaccgg
     1201 tgaaatcgtt caagaaatga ccacggatga aaacggtcat gcggaaacac caaaagagat
     1261 tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa actaaagcta gtcatgggtt
     1321 cgtgaatacg ttcaaacctg taaaagtcga attgaagtat gccaatcaaa ccgtagcccc
     1381 tgttaccagt aacgtaaaag ggcaaaatca agaagtcact ggcgaaacca ctttgacgaa
     1441 agaagacaaa gatactgggg ataaagcaca aggtaaggca gtctttgagg gtgctgaata
     1501 caccttgttt actgctaagg atggaaaagc tgttaaatgg agtgaggctt ttaaaccaga
     1561 gttagcgaag ggaacgaaag gttctgatga aacggtcact ttgactttag acgaaaagaa
     1621 ccaagttgcc gttaaacatt tagctatcaa cgagtattat tggcaagaaa caaaagcgcc
     1681 cgaaggatat agccttgatg aaacaaagta tcctgtatcc atcaaaaagg tggatgatag
     1741 cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa gaacaagtga ttcgctttgg
     1801 ctttgatttc tttaaatttg ctggatcagc tgcgggcact accgaaactg gcttcaatga
     1861 tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa atcacaggtg ccgaagatga
     1921 agcgaccaca gcttataacg agcaattagg gtttgatggt tatggcaagt ttgaaaatct
     1981 tccttatggg gattatttac ttgaagaagt agaagcccca gaaggttttc aaaagattac
     2041 gccattagaa atccgttcta cttttaagga aaacaaagaa gactttgtga agagtgagta
     2101 tgtctttacc attaccgaac aagaccaaaa acaaccaatt aaaacggtga ccgttcctta
     2161 cgagaagctg acgaacaaag cattttctgt tagtttgaac cgtttaatgc tctatgattt
     2221 gcctgaggaa gaagatagtt taacttctct tgcgacttgg aaagacggaa acaaagaatt
     2281 aacttctctg gattctaccg agctagttga taaattgagt tacaatctac atgaaatcaa
     2341 agaagactgg tatgtggtag cgcaagccat tgatgtggat gcgacaaaag ctgcccaaga
     2401 aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca agcgcaacgt tggcgaacaa
     2461 agagaaaaca ggaacttgga aaattcagca taaattaacc gccgaacaag ttttaaataa
     2521 aactattgtc ttattcaact atgtgtatga aaataaggaa gcttttgaag caggcgataa
     2581 gccagtagcg aaggatgtta gcttgaataa ccaagcccaa actgtcagtt gtacagtgga
     2641 acaccatgtt tctatccaaa caaaggccca cctagaaaac ggttctcaga cctttactca
     2701 tggtgatgtg gtggatatgt ttgatgatgt gtcgattact catgatgtac tggatggctc
     2761 aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg gatggtacaa acaaagaaat
     2821 ttggaaatct ggcaagattg attatgaagt aaatgataaa gaattcacca aaactgtgct
     2881 tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc aagtttactt ttgcggaaat
     2941 caattacgat aaagatggga atatcaatgg aaaacacaat gaagatttga aagaaaaatc
     3001 tcaaacctta acaccaaaag aagttccaac catactaagt acgccgaaac aaccggaaac
     3061 accaactgtt ccaagtggct ctcaagaatc tagtcccaca gtgaagacct tccctcaaac
     3121 cggggagaaa aattccaatg ttttactgtt cattggtttt actttgattt ttgcgacggc
     3181 gggctattat ttctggaatc gccggaacta aggtgatgag atgatacaag taaatcctaa
     3241 gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg ctatttttgt atccaaaact
     3301 aacaatgaaa agaggaaaag aagatggaat tgaaatttgt cgtgccagat atggctgaaa
     3361 cctttggaaa gttaagttat gccggtgaag gagaaattat aacagaagga tatggacgga
     3421 atactacggt gattggtcgc agttatcatt tgtattccag taagcaacgg gccgatgata
     3481 ttgaggtggt agtagctgcg gaagccggtg aaaaggattt tgatcaagac caaccgctaa
     3541 aagccgtgaa tccccatttg gttgccaaag gctatgagat tgagaatcgt gggtttactg
     3601 attacgtgtt gtatgttgat gatttagtga aagcatagga ggaaaagaag atgagattaa
     3661 cagaaggtat tgtcgtagat tcaggtttaa catttgggaa gttgcgtttt tccgcattac
     3721 gccgagaagt acggaagcaa aatgaggatg gaacaattgg taatgaggta aaagaacgaa
     3781 cctataattt aaaatcctct gcacaagggc gaatgattca agttagtatc ccagctaatg
     3841 tacccttacg cgagtttgct tatgatgcag aggtagagtt ggtcaatcca atcgtggata
     3901 ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa ttggttcatt aaggcggatg
     3961 atttagtctt gaagcggcag ccgaatcaag gaaatcctac gaaccaaaat gaaagaaaga
     4021 aataggtgaa gattatggaa attaatgttg aacatatttt agattgtctc gatcaatatg
     4081 gaaaaggcga gctgactgag gaacaactcg ttaaagcatt aacctctgat gaaaaaatgt
     4141 ttctgattat acatcaaggt ttacttggag aaaatagcga cagtgaagaa gactctgatg
     4201 tactagactg gttaggagaa gaagagtctt tctttatagt tgtcgaagta aatgaaaact
     4261 tatgtcggca ggcagaatcc attttggaag aaattggtgt ggagatgccg gatgctattg
     4321 aaggcttttt aaatcaattg gttgaaacga aacaactccc ctcctcgtag cagtaaatga
     4381 ctagcataac tttttgaaat tgcctaatga aatcctattg ggctttttcg attggaggtg
     4441 gaaaccatca aaatgtacaa aggacatcgc atacgcgcag gagatcagca cttggtttat
     4501 cactttgttc ttggctggct actcgctctg ttcatcggtt ggatgagcgt cttttatttt
     4561 caagagttta gacaatttga tatttctaag ctatcacttt ctactattga aatcgtttgg
     4621 tctataaaag atttagtatg tctgcttgga agtctcgctt tttcaggagc tatgatattg
     4681 ctttatattc acttttttct tgatcattgg cgaagcttgt ggcatcgaca aaagctagct
     4741 cggatgattc tagagaacca ttggtatgaa gtgaaacaaa ctcaaagtga aggctttttc
     4801 aaagatttga atagtagccg aaccagagag actatcagtt acttccccaa aatctgttat
     4861 cgaatgaaag atggattact gtctattcgc gttcaaattt cacttgggaa ataccaagat
     4921 cagcttttaa aattggaaaa gaagttagaa agcggactgt attgtgagtt agtagaaaag
     4981 gaactcaaag actcctatgt ggagtatact ttactgtatg atatgatcgc caatcgaatt
     5041 gggatagacg aagtagtggc agaaaatggg actctgcgat tgatgaaaaa tcaagtatgg
     5101 gcttatgatt ctttacccca tatgttaatc gctggtggca cgggtggagg gaagacctat
     5161 ttcctcctca ccattatcga agcactattg aagtcagatg cagaattgtt tatcctcgat
     5221 cccaaaaatg cagatttagc tgatttaggt acggtgatgc ctcatgttta ttcacaaaag
     5281 gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta tgatggctcg tagcaaggca
     5341 atgaaagaaa tgtctaacta caaaacagga gagaattatg cctatcttgg acttccaccc
     5401 aactttttaa tctttgatga atacgtggct tatatggaga tgttaacgac gaaagaaagt
     5461 gcggtgattt tgaataagct caaacaaatc gtgatgttgg gtcgtcaatc tggtttcttt
     5521 ctgattttgg cttgccaaag gccggatgcg aaatatttag gagacggaat ccgcgatcaa
     5581 ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg gatattccat gatgtttggg
     5641 gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc gaggttatgt ggatacaggt
     5701 ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa aagggtatga tttcttagat
     5761 tcaattagta aattagaaaa gatgaatgat tgagtgatct tcaatactga ctttatactt
     5821 caagtaacgt ataatacttt tatatagtag gaagaaaggg gataattaat atggaaagta
     5881 atgattctta tttagagtat gcaaaagcaa ataaggataa gtttatttct tatattatca
     5941 aagggaaatc ccccgaagtt ggacgagacg cagtttttat ggcagggagt cctggggctg
     6001 gaaaatcaga ggttgccatt ggattagctg agaattatga taaccatgtg attattgatg
     6061 cggattactt tcgcacccag tttcctgatt ataatggaaa aaactctagt ctctttcaaa
     6121 aagcatcttc ttggttggta gagcaggcat ttagatatgt cttagatcat ggctattctt
     6181 ttattctgga tgcaactttt gcaatattaa gtgctgaaaa aaatatctcg cgggcgttga
     6241 aaaaacattt tcgagtgacg attttctatg tttatcaaga ccctaaaatc gcttggaaat
     6301 ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc gaaaaaggca tttattaacg
     6361 cttttttcaa atcaagagag aacattgcaa aagtaaagag taaacatcca gatgtattgc
     6421 ttcatatcat aataaaagat tatacgaata atatttccga agttcattat gctacggata
     6481 atctagaatt atttttacca ctagggtata gtaggaaaga tttggaggtt caattacatg
     6541 actgagaaag aacaagtaac gaaaattgta aagaaataca ataagagcat tgcggattta
     6601 tcggaaaatg ctactgcaaa agagttcaaa acggtgatta aatatgtagc cgatcaggcc
     6661 aatgaaaaac aacggaaatt agttggatta aataaaaaat agtaggacaa attaataatg
     6721 tatgagccaa gagcaaaatg ttcttggctt ttttctttgc aatgaatgag gtaatgtatg
     6781 agatttttag atttattcgc cggtattggt ggctttcgtt taggtatgga acaggcgagc
     6841 catcattgta tcggtttttg tgaaattgat aaatttgcta gacgtagtta taaagcaatt
     6901 catgatacaa gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga tgagtttatt
     6961 caatccctcg gaccagtgga catcctttgc ggcggatttc cgtgccaagc tttttcaatt
     7021 gcgggaaagc ggcaaagatt ttccgatact cgaggtactt tattctttga aatcgctcgg
     7081 ttcaccactc ttctccgacc taagctttta ttccttgaga acgtccgagg attgctcaat
     7141 cacgaaggag gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgac
     7201 gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc aaagccggga gcggatattc
     7261 cttatcggac attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggaccgtct
     7321 tcaacatctg atcaaaacat cagaaatttg gtaaatatca atccttccaa tcgaggaatg
     7381 ggtggacaag tctatggttc agatggcgta gcgccaacct taacaggtga tgaaggaatc
     7441 aaaatcgcac tgccagcaag ggatggaatc tctgtagcag gactattacc gggaaagttt
     7501 gaacaaggta atcgagttta tgaagttact ggaaccgccc ccaccttgtc aacgaaacaa
     7561 ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat atcaagaggt gaagccagaa
     7621 gacagtgtga atctggcttt tcctaattcc acaagtcgcc gaggtcgtct gggaaaacag
     7681 agtgttcata ccttactaac aggagaccaa caagcgatcg tcacggatca gtatcaaatt
     7741 cggaagctaa cacctagaga gtgttggcga cttcagggct ttcccgattg ggcttttgat
     7801 cgagattctc aagtcaattc agatagtcag ttgtataagc aagccggaaa ctctgtaaca
     7861 gttccggtca tttttgatat tgccagaaga ttaaaagagt tggatgtaca tggtttatga
     7921 tgagttgatt ggagaaattt attgggtgat tggaaagatt cagtcagacc cagaattaga
     7981 agaacaattg cgacggttaa aatttgatat tcgcaagaat ggtgtcaaag tcgctggcga
     8041 tccatattta atgaatgagg agacggatgc tcgaattgaa ataaatcaag taattgcaga
     8101 atttgagcgg attgcggacc aagcaaaaga accagatatt cgtcaatact tgttagaaat
     8161 gaaagcagag ttggaagtaa atggtattac agatgagtaa cctaaaccaa cacaactgac
     8221 tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc ctccgtggtg ctgtgcctgt
     8281 tcatggacgg cgttagccga ccatgatgtc tcacccccgt atctaacagg ggggtacaaa
     8341 aacacaaaaa ataagtaagc aaccattaag aatgcttgtt gtatcaaaga tagtaagtaa
     8401 tttgtatagg tgttaactca tttgaaaaag ttaacacctt gaatttggag gtgagaactt
     8461 ggcacaacgg aatttagatt atcggctatt aaaagaccga cgcaacgaat acggcgtttc
     8521 acaaaataaa ttggctacgg cttgtggact tagtcggccg tacttaaatc agatagaaaa
     8581 tggtggcgta accgcatcta taaaaaccat gaggaaaatt tttgaccaac tggaaagctt
     8641 taatcccgat ttgcctttga cgttactgtt tgactatgta aggattcgct ttcccacaac
     8701 ggatgcacga aaaattattc aagaaattct ccatttgaaa tttgagtata tgctccatga
     8761 agattatgcc ttttactcct atcaagaaca gtatgtcatg ggtgatattg tggtgatgtt
     8821 gtctcatgaa gaagataaag gtgtgctttt agaattaaag gggcgaggtt gccgacaatt
     8881 tgaaactttt ttactcgctc aaaagcgtag ctggtacgac tttttcgagg attgtctaaa
     8941 ggccggtggc gtgatgaaac gcttggactt ggcaatcaat gatcgagtgg ggctattgga
     9001 tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt
     9061 taaaagttat cgttccgggg aacttttaaa ggctgatgaa aaggacggca tgggcaatac
     9121 cttatatatc ggcagtctca agagtgaggt ttatttctgt ttgtatgaaa aagactatga
     9181 acaatatatc aagttaggga ttccattaga ccaaactgag acgaaaaatc gttttgagat
     9241 tcgactgaaa aatgaccgtg cttttcatgc gattcaagat ttgttaaaag gtcgtagcat
     9301 tgagagcacc acgttttcca ttatcaatcg ttacttgcga tttgcggata aagtagaagg
     9361 taaaaggcga accaactggc ccttgaatga acaatggggg cgttttattg gacggaatcg
     9421 gaaagagatt caattaactt ccgagccaaa accgtatacc attgaacgaa ccttgaattg
     9481 gctaggccga caagtcgccc ctacgtggaa aatggctaag gagttggatc gattgaatcg
     9541 gacaacctat atccaggata tggtacagaa tgcacgctta tcagaccgac ataaaaagat
     9601 tttggagcaa caaagtatgg caattgaaaa tttgattata tgaaaagagg aatcgtcatg
     9661 ggaattgtaa aagatatttt tttggtattt ggtggcagta tgcttggtgt tactgtgatg
     9721 tgcttgatga atgcaagtgc agcagcggat cgagcaatgg aaaagaaagg aaagtgacaa
     9781 gatgaatttc ggacaaaatt tatgtgcgac tcgttcctta gtgaaaagct aaggcactaa
     9841 taaaagaacg aaaaagtttg gttattagga gaactgataa tctgacaggt ggaatgatga
     9901 agtaacgctc tgaaacgcct gccctgagcc tccgacaagc attgtatgag gaaactcaga
     9961 atgtttgaag ctcggtaaag acggctgaaa gataccgtaa tagttgatga acatatatct
    10021 tctatcgaaa actatccaga ggtgggtagc gtatccaaca ggtcgggggt ctataaaata
    10081 tctatggtta gaatgttcta taaggaactg acgaaacagc gaatgtacgg gtctaaattt
    10141 gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat
    10201 aggttatgaa atgccatata acgttacagg cggtatcaag ccagcaggct tatagctagt
    10261 gacctaagga tatatatgta tagataagat tatcggaacg aggaaaggta ttgggttctc
    10321 atcaagagaa taaactgacg aagaacaata agcagttgaa ctaatgctga aaagcagagg
    10381 gcgaacctaa gaaattcttg taatgagaat ggaggaatgc cctcaagtcg tgtatatatt
    10441 agaaaagtat tatgttgttt caataaggat tacgagtaag ataaaaaagc tcactcaatt
    10501 tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga
    10561 tttaagacac gctgaatatt acgaaatgca aaacaccttt gatgatttat atgccaaaag
    10621 taagaatgga gatattttca ctcatcttat ggatattatc ctatcgagag aaaatatatt
    10681 actggcatat cgaaatatca aagcaaatgc aggcagtaaa acagcaggaa cagacggtac
    10741 aataattaaa gatattggca aattgccagc agaaacagtt gtcaaaaaag taagatacat
    10801 tgttgcaggc agtccacatg ggtatcgacc taagccagtg aggcgaaaag aaataccaaa
    10861 accaaatggt aaaaccagac ctctaggtat tccttgtatg tgggacaggc ttatacaaca
    10921 atgtataaaa caagtattag aaccaatctg cgaagctaaa tttagtgaaa acagctatgg
    10981 ttttagacct aataggtcag ttgaaaatgc aataaaagca acctataacc gcctacaaat
    11041 cagccagcta cattatgtga ttgaatttga tattaagggt ttctttgaca acgtgaacca
    11101 ttccaagctg ataaaacaga tatgggctat gggaatacgg gacaagcact tgatattcat
    11161 tttaaaaagg atattaaaag ctccaatcaa aatgactaac ggtacaataa catatccaga
    11221 gaaaggaact ccgcaagggg gaataatatc cccactacta gcaaacattg ttcttaatga
    11281 gttagaccat tgggtagaaa gccaatggca ggaaaatcct gttacaaaaa actatgtggt
    11341 tcatatcaat aaaagcggta gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac
    11401 aaagttaaag gaaatgtata tggttcgtta tgcagatgat tttagagttt tctgccgcta
    11461 caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa tggattgaac aacgattaaa
    11521 attggaagta tcacaagaaa aaacaagaat tgtaaatgtt agaaaaagat actccgactt
    11581 tcttggtttc aagattaaaa tgataccgag aagaaagaag ttagttgtta aatcacatat
    11641 aagtgataaa caattcaaaa accaaaaaga caagctggta tcacaggcaa agaaaattgc
    11701 ttctccctca aaagggaaaa cggaattaga agaaacaaga ctttataatt caatggtatt
    11761 aggtatgcaa aattattatc agattgcaac gaatgtaaat ctggattgta tgaaactgaa
    11821 tagagctgta atggctatct tcactaacag attaggtaca aacaaaaggg gcagattgag
    11881 aaaaacaggc agacaactta cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct
    11941 tcggtatgtt gctggcgagg gcgaacctat tttccctgtt ggatatatcc aacacagaaa
    12001 accaatggca aaggtgtatt tagcgaattg ctacactcta gagggaagaa aatttatcca
    12061 caccaatcta agtgttgata aattactgat gtaccagtta atgaaattat ctttggaaaa
    12121 tcgcacaata gaatacgcag acaatcgaat ttcgctattt tctgctcaac atggaaaatg
    12181 tgctataact ctggaagaat ttcaacaagc taatgaaata cattgtcatc atattgtacc
    12241 aactggagct ggtggaaatg acgattataa aaatcttatt ttggtaaaag aggcggtaca
    12301 ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa tatatagctc tattgaagct
    12361 aaataaagtc cagctcataa gactaaataa atatagagta ttagctggca a
//
LOCUS       DAZKNM010000070        12216 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000070 DAZKNM010000000
VERSION     DAZKNM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.071"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..811)
                     /locus_tag="ACG8M9_001572"
     CDS             complement(<1..811)
                     /locus_tag="ACG8M9_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0378347.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGET"
     gene            complement(908..1408)
                     /locus_tag="ACG8M9_001573"
     CDS             complement(908..1408)
                     /locus_tag="ACG8M9_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0378348.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(1421..1642)
                     /locus_tag="ACG8M9_001574"
     CDS             complement(1421..1642)
                     /locus_tag="ACG8M9_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378349.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            complement(1647..1784)
                     /locus_tag="ACG8M9_001575"
     CDS             complement(1647..1784)
                     /locus_tag="ACG8M9_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0378350.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(1781..2965)
                     /gene="mobT"
                     /locus_tag="ACG8M9_001576"
     CDS             complement(1781..2965)
                     /gene="mobT"
                     /locus_tag="ACG8M9_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0378351.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(3221..3475)
                     /locus_tag="ACG8M9_001577"
     CDS             complement(3221..3475)
                     /locus_tag="ACG8M9_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378352.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(3500..4642)
                     /locus_tag="ACG8M9_001578"
     CDS             complement(3500..4642)
                     /locus_tag="ACG8M9_001578"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0378353.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(4702..6054)
                     /locus_tag="ACG8M9_001579"
     CDS             complement(4702..6054)
                     /locus_tag="ACG8M9_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0378354.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(6125..6469)
                     /locus_tag="ACG8M9_001580"
     CDS             complement(6125..6469)
                     /locus_tag="ACG8M9_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0378355.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(6479..6853)
                     /locus_tag="ACG8M9_001581"
     CDS             complement(6479..6853)
                     /locus_tag="ACG8M9_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0378356.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(6866..7180)
                     /locus_tag="ACG8M9_001582"
     CDS             complement(6866..7180)
                     /locus_tag="ACG8M9_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0378357.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(7294..10521)
                     /gene="fss3"
                     /locus_tag="ACG8M9_001583"
     CDS             complement(7294..10521)
                     /gene="fss3"
                     /locus_tag="ACG8M9_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0378358.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(10600..11262)
                     /locus_tag="ACG8M9_001584"
     CDS             complement(10600..11262)
                     /locus_tag="ACG8M9_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378359.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            11879..12109
                     /locus_tag="ACG8M9_001585"
     CDS             11879..12109
                     /locus_tag="ACG8M9_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378360.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
BASE COUNT         3429 a         2730 c         2026 g         4031 t
ORIGIN      
        1 gagtttcgcc tagcattaaa gcatcgcgaa gtgatcccaa gtcaggttcg tccttcattt
       61 ttatatagga atatcttgtg cctattcccc aaagtccatg ttttgaattt cctttcgagt
      121 gtgggttatc agaaaagcta agaaatgtca tattattttt ttctgcctca acaactgatt
      181 tataccaaga ttccaaagta tcttttccaa ctctaccatc atatctggca ttcacactat
      241 catagatagt gtcacgctta gatgagggaa tcatttgtga gcgattttca aaaaattctt
      301 tttgaaccgt ctgtactgca tatatatttt ctgattcaaa tatctttcct agatttccaa
      361 attttgctga agaaattcca ttaaactcat ctatatgtgc aggaataacg agcaagccct
      421 cgtgctcagc aaaatcaata acttctaaaa ctgatttcat tgagcaagct gcagcgctgg
      481 cccaatcctc tctttttata cccatcttgg ttaagaaatc agagatatca tttgtacatg
      541 cacttttatc aaataggact agtaaatgag ttccattttc tccacacgtt aattccactc
      601 caggaaaaaa atctaagcca ttttcgatag ccactttctt atattcatca atattttccc
      661 ctgtattgtg atcagttaat gcaacacaat cgagtccttt gtttaaacat tcttctaccc
      721 attccttagc agtaaccttt ttatcctgaa agcactgact cgcagttgaa tgaatatgaa
      781 gatcacattt gtaccactta ttgccaacca ttagtaacac ccctatcaac aaatattttc
      841 tatttcagta tatcaaatag aaaagtaaaa gtacgataga aatttctggt caattctaac
      901 aaatgattta cattagttca aaaacccctc gactcgtaac caaataattc ccgccaatct
      961 ccaagtctcg accacacgca tcaaaatcaa tatacgtctt caagtgttct ggtacttcgc
     1021 ctaaatcacc agcttctaca atcaagtaat acgctacatc actcatgtca ttacaatcgg
     1081 gataatagat gatatcatcc acatgctcta ccatttcctc gaaagaattg aagaaggcat
     1141 gttgaatctc tgaagcgacc tcaccaattg gcgttccttt aagctcttcc gctaaaccac
     1201 acaaccgatt aatttcctca attggtgtat actcatcaat ctcaaaaggc agttcataat
     1261 catgaattgc gtactcttca tattcgtcat ttaatccaat ccgttctttc acttcatcaa
     1321 aatcgacggg tggggtaaac catgccccca ccaactcacc ttcattgtat tttccaaggt
     1381 tcgcaatata aacacgtatc tgttccattg tgattccccc tcaggcaccg acaattcggt
     1441 taaacaaatt gagtaagaca tccttcacac cagaagcatt aaagaccaga cctaccgcaa
     1501 tcaaagcaat caccaagaag ccaatcagtt tggaaaactc gcgtttaaat cctaaaaaca
     1561 aaccaatgac cacaatcgct agtaagacta aagattgagc attggttaaa aaccattgat
     1621 acaaattttg accgaaattc atttcatcac tttcctttct tttccattgc tcggtccgcc
     1681 gctgcacttg catgcatcaa gcacatcaca gtaacaccaa gcatactgcc accaaacacc
     1741 aataaaatat ctttcacaat ttccatcacg attcctcttt tcatataatc aaattttcaa
     1801 ttgccatact ttgttgctcc aaaatctttt tgtgccggtc tgatagtcga gtattattta
     1861 ccatatcctg aatgtacgtt gtctgattca accgatccaa ctccttggcc atcttccacg
     1921 taggggcgac ttgtcgtcca agccagttca acgttcgttc aatggtataa ggcttcggtt
     1981 cagaagttaa ttgaatttcc ttctgattcc gtccaatgaa gcgcccccat tgttcattca
     2041 agggccagtt agttcgcctt ttgccttcca ccttatccgc aaatcgcaag taccggttaa
     2101 taatggaaaa tgtggtactt tcaatactgc gaccttttaa caaatcttga attgcatgat
     2161 aagctcgatc atttttcagt cggatttcaa aacgattttt cgtttcggtc tggtctaaag
     2221 gaatccctaa cttgatatat tgctcatagt ctttttcgta caaacagaaa tagacctcac
     2281 tcttaagact gccaatatac aaagtattgc ccataccatc tttatcatca ggtttcaaca
     2341 gttccccgga acgatagctt ttgaaggtac gaaacaagga gatacattct tccttctgac
     2401 attttttcgt taaatcagga atatctaaca ggccaactcg atcattgatt gccaggtcta
     2461 agcgtttcat cacgccaccg gcttttagac aatcttcgaa aaagtcatac cagctacgct
     2521 tttgggcgag taaaaaagtt tcaaactgcc gacaaccacg accctttaat tctaaaagga
     2581 cgcctttatc ttcttcatgt gacaacatca ccacaatatc tcccataaca tattgttctt
     2641 gataggagta aaaggcgtaa tcttcgtgga gcatataatc aaatttcaaa tggagaatct
     2701 cttgaatgat tttcctcgca tccgttgtgg gaaagcgaat ccttacatag tcaaacagta
     2761 acgttaaagg taactcggga ttgaaacttt ccagttggtc aaaaattttc ctcatggttt
     2821 ttgtcgatgc ggttacgcca ccattttcta tctgatttaa gtacgggcga ctaagtccac
     2881 aagtcgtagc taatttattt tgtgaaacgc catattcatt acgccggtct tttaatagcc
     2941 gataatctaa attcctttgt gccaagttct cacctccaaa tttaaggtgt taactttttc
     3001 aaatcagtta acatctacac aaattgctta ttatccttga taggacagcc attccttatc
     3061 gttgcttgct tattttttgt gtttttgtac ccccctgtta gatacggggg gtgagacatc
     3121 atggccggct aacgccgtcc ataaacaggc acagcaccac ggaggatttc tgcttcgcaa
     3181 atcccccgcg gtcagtgcct atcagtggtt tatttggccg ttactccgcg gtaataccct
     3241 caatttccaa ctctgctttt atctcaaaca agtattggcg gatatcaggt tctttagcaa
     3301 agtccgcaat tcgttccaat tcactgatga cttgattcag ctccattcga gcatccgttt
     3361 cctcatcaat tacatacgga tcatcaggga ctttcacccc gtttttgcga atatcaaaat
     3421 tgagccggta cagttcttct tccaattccg gatctgactg aatcttgcca atcacccagt
     3481 agattcccca atcaactcat cataaatcat ctgtttttac ctcctttaat cttctagcaa
     3541 tatcaaaaat gaccggaact gttacagaat ttccagcctg cttatacaac tgactatctg
     3601 aattgacttg agaagctcga tcaaaagccc agtccggaaa gccctgaagc cgccagcact
     3661 ccctaggtgt taactttcga atttgatact gatccgtgac gactgcttgt tggtctcctg
     3721 ttaataaggt atgcacactt tgttttccca gacggcctcg acgacttgtg gaattaggaa
     3781 aagccaggtt cacactgttc cctggcttca cctcttgata ttctgtttcg tttttccgaa
     3841 gcatgatctt cgttccgcct tgtttcgttg acaaagtagg cgctgttcca gttatctcat
     3901 aaacccgatt gccttgttca aaatttccca gtaacatacc cgctacagaa attccatcat
     3961 ctaccggcaa tgcgattttg attccttcat caccggtcaa ggtaggcgct acgtcatctg
     4021 acccatagac ttgtcccccc attcctcgat tggaagggtt gatatttaat aagtttctga
     4081 tgttttgatc agatgttgga gacgatccaa tgataggaaa tacttgttcg gtacatgcgt
     4141 ctcgagaatg tccgataagg aagatccgct cacggttttg gggaacgtag gcctttgagt
     4201 taagcacttg ccattccaca tcatacccca atccatccag cgttcggagg atcgtctcga
     4261 acgtagcccc tccttcgtga ttgagtaatc cccggacgtt ctcaaggaat aaaaacttag
     4321 gttggagaat agcggcgaac cgagcgattt caaagaataa agtacctcga gtatcggaaa
     4381 atccttgccg ctttcccgca attgaaaaag cttggcacgg aaatccaccg caaaggatat
     4441 ccactggtcc gagagattga ataaactcgt ctgatacact tgtgatgtca tgcatttcca
     4501 cctccttact tgtatcatgg attgccttat aactctggcg agcaaaatca tcaatttcac
     4561 aaaagccgat acactggtga cccgcttgtt ccatacctaa gcaaaagcca ccaatcccag
     4621 cgaataaatc taaaaatgtc ataaatcacc tcgtaaaaaa agcccaggat tcttgacact
     4681 cctaggctac atactattat tttatccttc cgtattaacg tttaatccac caatgtcgct
     4741 aatttccctt aagaaatcat atccttttgg tacaagtggc gtataaaact cactaatcac
     4801 actccctccc gtatctacat agcctcgacc tttgatacgc ttcataaaga attttttatc
     4861 aacctcgcca aacatcatgg aatatcccag ttcactcata cgacccaaag ccacccagaa
     4921 attaaattga tcgcgaatcc cgtctcctaa atatttcgca tccggtcttt gacaagctag
     4981 aatcagaaag aaaccagatt gacgacctaa catcacgatt tgtttgagct tattcaaaat
     5041 caccgcactt tccttcgtcg ttaacatttc catataaacc acgtattcat caaagattaa
     5101 aaagtttggt ggaagtccaa gaaacgcata attctctcct gttttgtagt tgggcatttc
     5161 tttcattgcc ttgctacgag tcatcatgcg ctcatagaaa tcttccacac aagcagaaat
     5221 ttcctccttt tgggaataga catgaggcat taccgtacct aaatcggcta agtccgcatt
     5281 tttgggatca agaacaaaaa gctcggcatc agattttaat aaagcctcga tgatagtgag
     5341 taaaaagtaa gtcttcccac cacctgtgcc gccagcgatt aacatatgag gtaaagaatc
     5401 ataagcccat acttgatttt tcatcaatcg caaagcccca ctttctgcca ctacttcgtc
     5461 tatcccaatt cgattggcaa ttatatcata cagtaaagtg tactccacat aggagtcttt
     5521 gagttccttt tctactagtt cacaatatag accactttct aacttctttt ccagctttaa
     5581 aagctgatct tgatattttc ccagtgaaat ttgaacgcga atagaaagta aaccatcctt
     5641 catacggtaa tagattttgg ggaagtaact gattgtctct ttggttcgac tactattgag
     5701 gtctttgaaa aatccttcac tctgagtttg ttttacttcg taccaatgat tttctagaat
     5761 catccgggct agcttttgtc gatgccacaa actgcgccaa tgatcctgaa aaaagtgtat
     5821 ataaagcaac atcgtgcttc ctgaaagaac gagacttcct agcaaataga ttaaatcttt
     5881 tatagaacga acgatttcta tagtagaaag tgatagctta gaaatatcaa attgtctaag
     5941 ctcttgaaaa taaaagacgc tcatccaacc gatgaacaga gcgagtagcc agccaagaac
     6001 aaagtgataa accaagtgct gatctcctgc ccgtatacga tgccctttgt acatttttat
     6061 ggtttccacc tccaatcgaa aaaagcttca taggatttca ttgatccaat ttcaagcagt
     6121 tatgctagtc atttactgct atagggagtt gtttcgtttc cactagttgc tttaaaaaaa
     6181 cttcaatggc atccggcatt tccacaccaa tttcttccaa tacggtttcg gcttctcgac
     6241 atagactttc atttacttcg ataaccataa agaaggactc ttcttcccct aaccaattca
     6301 gtacatcaaa atcttcttcg gcatcgttac ctacttccag taacccttga tgcataatca
     6361 gaaacatttt ttcatcatag gttaatgctt tagtgagttg ttcctccgtt agctcacctt
     6421 ttccatattg atcgagacaa tctaaaatat gttcaacatt aatttccata cgcttcacct
     6481 atttctttcc ttcattttgg tttgtagaat ttccctgatc tggttgtcgt ttcaagacta
     6541 aatcatcggc cttaatgaac caattgacag tagttccttc tcggaaaaca taattggcga
     6601 ccgtatccat gattggattg accaactcta cctctgcgtc ataggcaaac tcgcgtaagg
     6661 gtacatttgc tgggatacta acttggatca ttcgaccttg cgcagaggat ttcaaattat
     6721 aagtccgttc ttttacctcg ttactaaccg ttccatcctc attttgcttc cgtacttctc
     6781 ggcgtaatgc ggaaaatcgt aatttcccaa acgttagtcc tgaatctaca acaatacctt
     6841 ctgttaatct catcttcttt tcctcctatg ctttcactaa atcatcgaca tacaacacgt
     6901 agtcggtaaa cccacgattc tcaatctcat agcctttggc aaccaaatgg ggattcacgg
     6961 cttttagtgg ttgatcttga tcaaaatcct tttcgccggc ttccgcagct accaccactt
     7021 caatatcatc ggctcgttgc ttactggaat acaaatgata actacgacca attactgtag
     7081 tattccgccc atatccttca gttaaaactt cgccttcgcc ggcatacctt atctttccaa
     7141 aggtttcggc catatctggc acaacaaatt tcaattccat cttcttttcc tcttttcatt
     7201 tttagttttg gatacaaaaa tagcgcgacc acttttttta ggaggcggtc gcgcacgttg
     7261 gtttttccta tttagttgtt tcatcacatc accttagttt cggcgattcc agaaataata
     7321 cccagcagtc gcaaagatca aggtaaaccc aataaacagc agaacattag aatttttctc
     7381 ccctgtttgt gggaatgtct ttactgtagg actagattct tgagagtcac ttggaacagt
     7441 tggtatttcc ggttgttctg gtgcacttgg tgttgtcgga acttttttag gtgttaaagt
     7501 ttgagttttt tcttttagat cctcattgtg tttcccattg atgtttccgt ctttgccata
     7561 gttaatttct ttgaaggtaa aacttgtacc ttctggatat ttacttgtgt ccactttttc
     7621 cgcaagtacg gttttagtga attctttgtc attcacttca taatcaattt tgccagattt
     7681 ccaaatttct ttggtcgtat tatccggaag taatgcatac agaattgttt caaaagcttc
     7741 ttttgagcca tccagtacat catgggtaat cgacacatca tcgaacatat ccaccacatc
     7801 cccatgagta aaggtttgcg aaccatcttc taggtgggct tttgtttgga tggaaacatg
     7861 gcgttccacc gtacaattga cagtttgggc ttgattgttc aagctagcat ctttcgctac
     7921 tggatcatca cctgcttcat agggtttctt gttttcatac acatagttga ataagacgat
     7981 ggttttatcc aaaacttgtt ctgcggttaa tttatgcaaa attttccaag ttcctgtttt
     8041 ctctttattc gccaacgttg cggttgtttc ggcaattacc actggtttgg ctttttcatc
     8101 tttttcttgg gcagcttttg tcgcttccac atcaatggct tgcgctacca cataccagtc
     8161 ttctttgatt tcatgcaagt tatagctcaa tttatcaact agctcggtag aatcaaggtc
     8221 tgtcaattct ttgcctccgt ctttccaagt cccaagagaa gttaagctat cttcttcttc
     8281 aggtaaatca tagagcatca aacgattcaa gctaacagaa aatgctttat tcgtcagttt
     8341 ctcgtaagga acggtcaccg ttttaattgg ttgtttttgt cctttttcgg taatcgtaaa
     8401 aatgtattca ctcttagtgt aatccgcttt attttcttta aaagtagaac ggatttctaa
     8461 tggcgtaatt ttttgaaatc cttctggagc ctctacttct tcaagtaaat aatccccata
     8521 aggaagattt tcaaacttgc catagccatc aaaacctagt tgctcgttat aagctgtaat
     8581 tgcttcatct tcggcatctg taatctcact ggttccttcc aatggcgata ctttaaaggt
     8641 cagatcattg aagccagttt cggcagtccc agcagctgat ccagcaaatt taaagaaatc
     8701 aaagccaaag cgaatgactt gttcttttgc cgtaacatct cgggtaatca cggcattttt
     8761 ttcgttatca tccacctttt tgatggaaac agggtacttt gtttcatcca aggtatatcc
     8821 ttcaggtgct ttggtttctt gccagtaata ctcattaatc gctaagtgtt taacagcaac
     8881 ttgattcttt tcatctaaag tcaaagtcac cgtttcatca gaagctttcg ttcccttcac
     8941 taactctggt ttaaaagcct cactccattt gaccgcttga ccatcttttg cagtaaagag
     9001 agtatattca gctcctttaa actcagcttt accttgagtt tcattaccgg tatctttgtc
     9061 ttcttttgtc aaagtggttt caccagtgat ttcttggttt tgccctttta cgttactggt
     9121 aacaagagcc acggtttgat tgacatagtt caattcgact tttactggtt taaaggtatt
     9181 cacaaagcca ttactagctt tggtttcggt cacataatag gttcccaatt ccaacgcatt
     9241 ggcaatctct tttggtgttt ccgcatgacc tttttcatcc gtggtcattt cttggacaat
     9301 ttcaccggtt ggactgtctt tgcgaatggc aaacgtattt ccggctaatg agtatttgtc
     9361 gttccaaaga tcagttcctg tttccacccc cattttattc aaaatgattt gacctttttc
     9421 gcgagtattt tttgaagtca cagaaatagt ctcaccagat ttaatggttg cggtgattgg
     9481 tgtagtgtca atcgtataag gagctggcac tgatttttca gtaatggtta ctttcgcgcc
     9541 atgaggaatc tcatccaatg tcgcaatgcc ttctttatcg gtggtcacgt cttttgcagg
     9601 taatgttttt ccaaagtcta aatggaaaac ggttcccggt acaacatttc ctgtttcttt
     9661 atcgactttt ttaatcttca aagaaccctc agtttccaca tcaattttga ttgtgtaggt
     9721 attgggctta tcaattgccc ccgccatcaa agtttgttga ccagccattt tataagctac
     9781 cggtgttcct gtattagctg acttctttag ggttaattca ccactctctt ttgaattggc
     9841 atttggagta atcactagtt gattgccgtt cacacgataa tcaatatttg cgatattctc
     9901 gacaacttca tcaaactccg ataaattcaa gccgttcgta tctgtcacag tagtcgattg
     9961 acctaacacg gttttcactg tggagttatg gaagcttggt ttcttttgat aatccgcaat
    10021 ggcttgattg atttttgact caatggctgc gatatttact gcactaccat cagaacgttt
    10081 aattgagtga agcgtgtagc cattaacttc ttgccaaatc atcttctggg ccacaatatg
    10141 tgtatccaca tccgtaccag cttccttcca taaaacggaa actaatttcg ctttctccga
    10201 catatcaggc aatggatttt tctcataacc aggagtgaac tcattgtaaa tcggcacccc
    10261 tggctcaata cagaaaatat cttgcttcac accattgtaa actgcatagt gcggaacggt
    10321 aacttctgac atgggacgac catcgctaaa tgtaccttcg acagcatact ggtgagccaa
    10381 atccaattcc acagttaccg tttgtggatg agtaatttca tcagcaaagg caatcgttgg
    10441 gccaagcgat ccaccaattg tttggcataa tagactcagt gttgcgagca aacgccaatt
    10501 cttaaatctt ggctttttca taaaataaat ctcctttcgt ttttgggtaa taaaaaagac
    10561 gtccaaattt taattttgaa cgtctgattt agagtgatac tatttagttt tctttgtctt
    10621 ggataggttg tttctctgct tataaatttt gattcttcga taatcaaatc caatctctgc
    10681 catcaattcc tcccaatttt tgttcgttct tttcataatt gtatcaactg atggtagttt
    10741 tggggattga actagttttc gaaactttgt cattgataaa tcatcctgta gttttaatcg
    10801 gattatttca ttttttagtt ctaacagcag ttcgaaatca gtaaatcgag atccatcatt
    10861 tttttcttta aaaagtggac gtatatctcc taacatcttt ttaatggtac taagtgaagg
    10921 aatattcttc cctactgatt tttgttcgta cattcgttga gaggttatct tctcgcactt
    10981 aataaaggct tctactattt ccaataattc ttgttctgac attctagccc attttccata
    11041 cccagtattt tctcgtccta tactaatcaa taaattctcc catgatccat attttttgtt
    11101 aatgaaccac atacttggtg cagaatttgg attttctgat agctttttct ggtattgacg
    11161 gctagatgta attccgtgtt tttcgatgaa ttcattaatt gttaaaatca gttttaactc
    11221 actttgattt ttccaaatga tacgtccatc attttgcttc acgataaaaa ctcctaatag
    11281 ttatttagat agaagcctat cacatcaact ttttagtaga aaagtacatg ttttgtcttt
    11341 tttgctaata gctgaccctt tgagaaactt gggttcaaaa gttgctaaat tcggtaaatg
    11401 ttttggtatt tttcgtatta tatttgtcgt caagctgact actaaatttt gccctatttt
    11461 gacgacactg atagtcaatg tcggacaatc taggacaatt tttaagggaa tatgattgaa
    11521 aaatggtcca aaacagccaa aaatggctta aaactgtgat agagaaaaaa gaaaaagccc
    11581 ttgatataca aggacttttc acaaatttca gatttccaag gaagttttcc gtgtttatct
    11641 tctgatttct ttgatacgag cagcttttcc gtgtaatgca cgtaaacaaa accactatat
    11701 tctattatgt atctaatacc ctcaaaacag ctataaatca acaataccga catataataa
    11761 acactatgtt caatctgaga tatatcaaag gtaaacgtat ttttgccccc tttttgcccc
    11821 tcaaatatcg ctttacaacg agaacgtaag ttcgtataat cgttttgagg tgatttttat
    11881 gatggaagac tttattagaa aaaatatcag tgatgaatat gcagactttt atgagcaaag
    11941 cagcaaaaaa gataaattcc agatggatgt ttcaatctta gctatattag ctttttccga
    12001 aaataaccaa cctgtaattg cgaaaaaaga aacagtatta tctgaaggca aaataaaaac
    12061 tcgatatata ttagaggtag aaactaagtt taaaaataga tcggagtaat ggctatgctt
    12121 tttaatgaga cacaattatg gtttaaattt gatccttcga atagatttat caaagatttt
    12181 tataaggtgt gggattcaga agttttcttt ctagca
//
LOCUS       DAZKNM010000071        12188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000071 DAZKNM010000000
VERSION     DAZKNM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.072"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..638)
                     /gene="frr"
                     /locus_tag="ACG8M9_001586"
     CDS             complement(81..638)
                     /gene="frr"
                     /locus_tag="ACG8M9_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0378361.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(641..1363)
                     /gene="pyrH"
                     /locus_tag="ACG8M9_001587"
     CDS             complement(641..1363)
                     /gene="pyrH"
                     /locus_tag="ACG8M9_001587"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0378362.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1499..2380)
                     /gene="tsf"
                     /locus_tag="ACG8M9_001588"
     CDS             complement(1499..2380)
                     /gene="tsf"
                     /locus_tag="ACG8M9_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0378363.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2479..3261)
                     /gene="rpsB"
                     /locus_tag="ACG8M9_001589"
     CDS             complement(2479..3261)
                     /gene="rpsB"
                     /locus_tag="ACG8M9_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0378364.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3620..4099
                     /locus_tag="ACG8M9_001590"
     CDS             3620..4099
                     /locus_tag="ACG8M9_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0378365.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4316..5407
                     /gene="phnW"
                     /locus_tag="ACG8M9_001591"
     CDS             4316..5407
                     /gene="phnW"
                     /locus_tag="ACG8M9_001591"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0378366.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5400..5528
                     /locus_tag="ACG8M9_001592"
     CDS             5400..5528
                     /locus_tag="ACG8M9_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0378367.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5532..6077
                     /locus_tag="ACG8M9_001593"
     CDS             5532..6077
                     /locus_tag="ACG8M9_001593"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0378368.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6531..8222)
                     /gene="argS"
                     /locus_tag="ACG8M9_001594"
     CDS             complement(6531..8222)
                     /gene="argS"
                     /locus_tag="ACG8M9_001594"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0378369.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8642..9589)
                     /gene="arcC"
                     /locus_tag="ACG8M9_001595"
     CDS             complement(8642..9589)
                     /gene="arcC"
                     /locus_tag="ACG8M9_001595"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0378370.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9704..10723)
                     /gene="argF"
                     /locus_tag="ACG8M9_001596"
     CDS             complement(9704..10723)
                     /gene="argF"
                     /locus_tag="ACG8M9_001596"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0378371.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10814..12043)
                     /gene="arcA"
                     /locus_tag="ACG8M9_001597"
     CDS             complement(10814..12043)
                     /gene="arcA"
                     /locus_tag="ACG8M9_001597"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0378372.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3339 a         2528 c         2202 g         4119 t
ORIGIN      
        1 ttttttaatt tttaaataaa agaaatataa ataaaaacag aggaagcgga atgatccgcc
       61 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
      121 ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      181 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      241 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      301 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      361 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      421 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      481 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      541 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      601 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      661 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      721 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      781 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      841 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      901 cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      961 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
     1021 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1081 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1141 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1201 cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1261 tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc
     1321 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1381 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1441 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1501 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1561 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1621 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1681 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1741 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1801 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1861 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1921 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1981 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     2041 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2101 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2161 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2221 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2281 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2341 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2401 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2461 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2521 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2581 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2641 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2701 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2761 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2821 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2881 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2941 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     3001 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3061 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3121 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3181 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3241 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3301 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3361 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3421 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3481 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3541 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3601 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3661 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3721 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3781 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3841 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3901 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3961 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     4021 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4081 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4141 tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt
     4201 attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4261 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4321 taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4381 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4441 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag
     4501 ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4561 ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4621 ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct
     4681 gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4741 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat
     4801 agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga
     4861 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4921 tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct
     4981 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     5041 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5101 aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat
     5161 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5221 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt
     5281 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5341 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5401 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5461 ctgtccacgc ttttagaaat gcttttctag aaaaaggaac ccaactgact gacaaagaaa
     5521 tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5581 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5641 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5701 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5761 cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt
     5821 attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt
     5881 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5941 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     6001 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6061 ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat
     6121 ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca agaatccaga
     6181 atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac
     6241 agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca
     6301 agcttttgaa caccgtttat tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6361 gtccccttat ttgcaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6421 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6481 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6541 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6601 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6661 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6721 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6781 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6841 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6901 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6961 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     7021 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7081 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7141 ggaatatggt gcatgtcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7201 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7261 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7321 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7381 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7441 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7501 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctctttatcg
     7561 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7621 gctacttcat ggaattggac gtataaacga agcaattcat tgattggttc tgctttaaca
     7681 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7741 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7801 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7861 gaagacatgt cgattggtac tgttccttga ttcccgatgt tgctatcccc ataatgttct
     7921 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7981 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     8041 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8101 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgctc gacttgttcc
     8161 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8221 atctttattc ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8281 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8341 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8401 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8461 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8521 tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa aaaaatccag atggcctaaa
     8581 ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa
     8641 attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta
     8701 aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta
     8761 ctttcggaag catgcttcct ggagcgaatt gcttttcatc aatgtattgt ttcatttctg
     8821 aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt
     8881 ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag
     8941 caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag
     9001 gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga
     9061 cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc
     9121 cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt
     9181 ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca
     9241 caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt
     9301 cgcccatggc gttttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat
     9361 ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac
     9421 cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca
     9481 ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag
     9541 acaagatggc attgccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc
     9601 tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc
     9661 ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa
     9721 acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt
     9781 ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga
     9841 tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca
     9901 agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga
     9961 tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata
    10021 cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa
    10081 tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt
    10141 caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga
    10201 ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt
    10261 ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc
    10321 attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca
    10381 ttttttgact gaatccacgg aattcgattc cgtcaaacat acgtcctagt actttagctg
    10441 tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga tattcaggat
    10501 gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt
    10561 caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca
    10621 agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt
    10681 tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac attctccttt
    10741 agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag taagactgcc
    10801 gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac
    10861 caccacgacc tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac
    10921 gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg
    10981 ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac
    11041 cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt
    11101 tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct
    11161 taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa
    11221 atacggtatc caaatgcatc attgcacggt tgttcggaat tttgattgcc aacacttttt
    11281 caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag
    11341 ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt
    11401 cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg
    11461 gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca
    11521 tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt
    11581 ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatca tatagactgc
    11641 tagaacgtac ttcgatatct tttgtacgaa ccccagccat gattttatct accatttctt
    11701 gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat
    11761 gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt
    11821 ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat gtcttcgcaa
    11881 agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat
    11941 ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt aaaactgtct
    12001 ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc
    12061 tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctcat
    12121 tcaaaatgag acattcctca cattaatatt cgataattaa ataattaaac gtttttctat
    12181 ttttaagc
//
LOCUS       DAZKNM010000072        12167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000072 DAZKNM010000000
VERSION     DAZKNM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.073"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..310)
                     /locus_tag="ACG8M9_001598"
     CDS             complement(32..310)
                     /locus_tag="ACG8M9_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378373.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(425..1042)
                     /locus_tag="ACG8M9_001599"
     CDS             complement(425..1042)
                     /locus_tag="ACG8M9_001599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378374.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1437..2261)
                     /locus_tag="ACG8M9_001600"
     CDS             complement(1437..2261)
                     /locus_tag="ACG8M9_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0378375.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2604..3020
                     /locus_tag="ACG8M9_001601"
     CDS             2604..3020
                     /locus_tag="ACG8M9_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0378376.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="ACG8M9_001602"
     CDS             3017..3487
                     /locus_tag="ACG8M9_001602"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0378377.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="ACG8M9_001603"
     CDS             3501..4274
                     /locus_tag="ACG8M9_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0378378.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="ACG8M9_001604"
     CDS             4288..5100
                     /locus_tag="ACG8M9_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0378379.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="ACG8M9_001605"
     CDS             5154..6119
                     /locus_tag="ACG8M9_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0378380.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6255..8978
                     /locus_tag="ACG8M9_001606"
     CDS             6255..8978
                     /locus_tag="ACG8M9_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0378381.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8991..9623
                     /locus_tag="ACG8M9_001607"
     CDS             8991..9623
                     /locus_tag="ACG8M9_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0378382.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9831..10055
                     /locus_tag="ACG8M9_001608"
     CDS             9831..10055
                     /locus_tag="ACG8M9_001608"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0378383.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10157..10390
                     /gene="relB"
                     /locus_tag="ACG8M9_001609"
     CDS             10157..10390
                     /gene="relB"
                     /locus_tag="ACG8M9_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0378384.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10380..10649
                     /locus_tag="ACG8M9_001610"
     CDS             10380..10649
                     /locus_tag="ACG8M9_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0378385.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10828..12129)
                     /locus_tag="ACG8M9_001611"
     CDS             complement(10828..12129)
                     /locus_tag="ACG8M9_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0378386.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3973 a         1973 c         2387 g         3834 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     6301 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagctcta cagttgaata
     6361 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     6421 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     6481 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     6541 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     6601 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     6661 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     6721 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     6781 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     6841 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     6901 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     6961 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     7021 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     7081 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagccaaa
     7141 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     7201 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     7261 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     7321 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     7381 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     7441 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     7501 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     7561 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     7621 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     7681 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     7741 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     7801 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     7861 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     7921 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     7981 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     8041 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     8101 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     8161 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     8221 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     8281 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     8341 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     8401 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     8461 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     8521 agattccata tattggatta gatcgcctga ttaatggggg aagcattggc gaatttgttc
     8581 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     8641 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     8701 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     8761 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     8821 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     8881 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     8941 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     9001 gagtaatttt tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa
     9061 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     9121 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     9181 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     9241 ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     9301 cactggcatc ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta
     9361 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     9421 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     9481 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac
     9541 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     9601 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     9661 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     9721 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     9781 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga
     9841 accaaaatac gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata
     9901 ctgatactac tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga
     9961 tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga
    10021 gattttgcga taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt
    10081 ttgtactaac gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat
    10141 gaacggaggt agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa
    10201 agaactggta ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa
    10261 tgctgtactg gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga
    10321 agtttccaaa gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa
    10381 tgaattataa agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa
    10441 accaagctaa aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc
    10501 gtcagcacgg aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa
    10561 actatcgaat attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg
    10621 gacatagaag tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag
    10681 tcaatgtata atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc
    10741 ggttggtttc tgctttttta tggctctttg tcaaatagga ctgatagagt gagataagaa
    10801 catctaaggt agaattcctt ttctacttta gatgtttttt gattcatagg taatcagatt
    10861 atttagtaag aaaattctgg tcttcatttt ccgaaagctt cgaaagccat aagcgactcg
    10921 tttcaatgct ttgatgtggg tatgcagatt ttctattttg gcatttgaat aaggcaattc
    10981 gagggctaaa cgaatgccgg attcatagtt tataaaagct ttttttatag aatgcagttc
    11041 ttttggaacg gaattgggca tcgttcttac aagtttaaag aaagtgtccg cctctttgtc
    11101 gtgaaacgct tgaagaattt cttgatagta aaaataggcg attcgtaatt cgtcagagat
    11161 cgaaagcaga cgatccacca cttcactttc ggtgagatac gcccaaccaa aattgcgcca
    11221 tttggtccat ttgtcataaa caagcttgtc ctgtcgtttg atcagaagcc gagacaacgc
    11281 ctttaattgt cgataatatt tggcttgtgt tggatcgttt cgatcaaagc ttttcatgat
    11341 tcgtacgcga atatcctcaa atttacgaat gagatgtttg acaatgtgaa aacgatccac
    11401 aacgagtttt gcctttggaa aacattcctt tatcagagag gcataagaaa cattcatgtc
    11461 cgtcacgatg acctttacct tcaatcgtgc gcgtcgttca aattgcataa aataagaaac
    11521 gagagccttt ttcttccgtg aaggaagaat atccagtaat tttccagtat cgccatccat
    11581 acaagaaaag gagtagggac cgacttgatt tttcagtgaa cgaaattcat ccattaataa
    11641 acaacgcggt aaccaatttc tttttacttt tgtcatgggg atcagagaag cgagtgtccg
    11701 agaaacggtc gaccaagaaa cgccgcaatg cttagcaatt gtcgtcattg cttgtttttc
    11761 agttagctct tctaaaattt tatagcgcac ttgtttagcg ataaagcagt ttctatcgac
    11821 aatgtaggta ttcgccgtaa aataggtgtg gcagccacga cagtggtagc gttgtttcgc
    11881 taaagcaaga taagtcagtt ggttcgcaca tttattaagt tgaatgagcg attttttctt
    11941 tccgttctta acgatcatgc cttcatgatt catctgttta cacaaaggac agcgacgaat
    12001 ttctttactt aacgtcgcag aaacaacaag actcttgata ttcttaatgc tcttcgattc
    12061 cacatttgtg atgaaaatgt ttttatcttt gattccaagt agtagtttga tatgatgaag
    12121 atcaggcata cagaacgctc ctttgattgt gttgtggtga ttcaatt
//
LOCUS       DAZKNM010000073        11947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000073 DAZKNM010000000
VERSION     DAZKNM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.074"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(157..321)
                     /locus_tag="ACG8M9_001612"
     CDS             complement(157..321)
                     /locus_tag="ACG8M9_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378387.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            616..1944
                     /locus_tag="ACG8M9_001613"
     CDS             616..1944
                     /locus_tag="ACG8M9_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0378388.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2146..2979
                     /locus_tag="ACG8M9_001614"
     CDS             2146..2979
                     /locus_tag="ACG8M9_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0378389.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2995..3732
                     /locus_tag="ACG8M9_001615"
     CDS             2995..3732
                     /locus_tag="ACG8M9_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0378390.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3887..4855)
                     /locus_tag="ACG8M9_001616"
     CDS             complement(3887..4855)
                     /locus_tag="ACG8M9_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0378391.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5087..5920
                     /locus_tag="ACG8M9_001617"
     CDS             5087..5920
                     /locus_tag="ACG8M9_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0378392.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5954..6793
                     /locus_tag="ACG8M9_001618"
     CDS             5954..6793
                     /locus_tag="ACG8M9_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0378393.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6819..7379
                     /locus_tag="ACG8M9_001619"
     CDS             6819..7379
                     /locus_tag="ACG8M9_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0378394.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7578..9299
                     /locus_tag="ACG8M9_001620"
     CDS             7578..9299
                     /locus_tag="ACG8M9_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0378395.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9464..10606
                     /locus_tag="ACG8M9_001621"
     CDS             9464..10606
                     /locus_tag="ACG8M9_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0378396.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10622..11833
                     /gene="thiI"
                     /locus_tag="ACG8M9_001622"
     CDS             10622..11833
                     /gene="thiI"
                     /locus_tag="ACG8M9_001622"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0378397.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4064 a         2019 c         2483 g         3381 t
ORIGIN      
        1 tcgagtccta ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg
       61 ggtttctttt tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg
      121 taatctttac gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct
      181 aaatacgaaa taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg
      241 gatgacattc ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc
      301 gccagcaatc aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct
      361 ttcaatgagt attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat
      421 tatacaggcc attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat
      481 accgtatgac atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca
      541 tcatagaaga gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt
      601 tggaaggatg attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag
      661 tttcaagcaa atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact
      721 gcttctgctg aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg
      781 gctacgggga aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg
      841 aacacattcc gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa
      901 aattacactt ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt
      961 gcaacaggaa aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa
     1021 caagatggcg gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct
     1081 aaatcggtga tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg
     1141 aacaaattac tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca
     1201 ttagacgcta ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct
     1261 atctcattag gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac
     1321 tatcatcttg accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa
     1381 ttagatgatt atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct
     1441 tatacagtag agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt
     1501 gatatggaaa ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg
     1561 tttggttgtg atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct
     1621 tattcaatgg atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat
     1681 tttggagaaa gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg
     1741 caatcaacaa aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc
     1801 tttgttatga gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat
     1861 ttattgacga aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg
     1921 gatccaatgg gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa
     1981 aaagagctgg cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt
     2041 ccctgtgcta taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa
     2101 atatgatgac gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact
     2161 gttttatctg atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat
     2221 cagacctatc gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta
     2281 aaaaaaggat tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt
     2341 gtaccggata cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg
     2401 atcgagccgg gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag
     2461 caaacagcgc cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa
     2521 aattcttttg aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt
     2581 tctttggcag aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt
     2641 aaagaacgtg tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga
     2701 gacctgtgga gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca
     2761 gctgtttctt atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc
     2821 cgaccggaat ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac
     2881 cgactgccga tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt
     2941 tacggttccc aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca
     3001 aaaaaatata ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg
     3061 acatttcctt cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg
     3121 ggaagaggca gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc
     3181 atggagatta atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg
     3241 gagtacaata agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa
     3301 ttagtcaaaa tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt
     3361 gttaatgaag agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag
     3421 gaaaaaattg aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat
     3481 atcgatagta atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta
     3541 ggatatgact ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa
     3601 gtagagtacg ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc
     3661 aaaacaaaac atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg
     3721 aaaaataaat aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat
     3781 ttacgaaaat tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt
     3841 tttgtcacag cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa
     3901 acgaaatcaa ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg
     3961 ctttttgact attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc
     4021 cgccttcata atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta
     4081 cagggatttt ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc
     4141 ccgcacctaa agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata
     4201 agtccccttg catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa
     4261 tgtccagtcg ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta
     4321 agccgatggg acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg
     4381 tcacgtagcg tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg
     4441 cttcttcaca acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa
     4501 gttcattact tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg
     4561 tatcaagaaa aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg
     4621 atatacctga tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca
     4681 tgatgtgtgg tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga
     4741 ttgtgatttc tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca
     4801 tgttttcttt catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac
     4861 agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga
     4921 gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat
     4981 taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct
     5041 tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg
     5101 attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga
     5161 atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa
     5221 gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt
     5281 ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat
     5341 gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt
     5401 tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa
     5461 acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta
     5521 cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc
     5581 catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa
     5641 tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga
     5701 taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact
     5761 agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa
     5821 taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa
     5881 gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa
     5941 aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa
     6001 tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat
     6061 tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt
     6121 ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat
     6181 tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa
     6241 ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt
     6301 aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg
     6361 tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac
     6421 gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca
     6481 gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt
     6541 agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt
     6601 cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct
     6661 gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc
     6721 agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg
     6781 tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc
     6841 aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata cccacggctt
     6901 ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg
     6961 ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag
     7021 cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg
     7081 ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct ttttcattac
     7141 taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat
     7201 ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact
     7261 ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag
     7321 aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat
     7381 cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag
     7441 ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga
     7501 cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga
     7561 aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta
     7621 tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat
     7681 tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg
     7741 atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc
     7801 aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag
     7861 tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag
     7921 aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat
     7981 tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag
     8041 aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc
     8101 aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg
     8161 gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg
     8221 aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac
     8281 aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag
     8341 aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca
     8401 atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga
     8461 ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta
     8521 accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga
     8581 ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac
     8641 gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga
     8701 tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag
     8761 tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa
     8821 aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga
     8881 aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt
     8941 ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca
     9001 tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga
     9061 agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc
     9121 gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca
     9181 ataaagaata tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag
     9241 aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa
     9301 gcaaaaaagt tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt
     9361 ttgtatattt gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac
     9421 aaccgttata atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa
     9481 cagtgcaaca accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg
     9541 gattattggg aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca
     9601 gcaagcaaga aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac
     9661 ttcaggcgga acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga
     9721 atatgggaat catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga
     9781 acaattaaaa gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt
     9841 acgtgtcgac gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc
     9901 tgtgaataat gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga
     9961 ttttcctaaa attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc
    10021 gcaatggttg acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc
    10081 acggggaact ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg
    10141 tggagggcaa gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat
    10201 ggttaaagct ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg
    10261 cttaaaagta gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga
    10321 aggaactgac tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt
    10381 tctcgtccat gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc
    10441 acgtaaacat atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac
    10501 atctgctgtt cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat
    10561 gattattttc aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct
    10621 tgtgaaatac agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg
    10681 aaaaagcttt atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga
    10741 aatcaaaatc catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca
    10801 gctagttatt ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg
    10861 tgtagaaaaa gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta
    10921 tactgggaaa gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac
    10981 aagtaatgaa ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca
    11041 agttcaaatg cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta
    11101 tctatcgtat gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg
    11161 aatgcttatg ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg
    11221 gggagtggaa attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact
    11281 gcaaaaagca aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat
    11341 cgaagtgcca tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg
    11401 gatgacaatt actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca
    11461 tggtttagtc attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag
    11521 tatggtggct atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga
    11581 taaacttgaa atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc
    11641 ttttgaagat tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga
    11701 aaaggtccat caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc
    11761 tggcttgaag atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc
    11821 agaattgcta taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac
    11881 gaaaaaatag ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga
    11941 ataccat
//
LOCUS       DAZKNM010000074        11853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000074 DAZKNM010000000
VERSION     DAZKNM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.075"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(213..2288)
                     /gene="glyS"
                     /locus_tag="ACG8M9_001623"
     CDS             complement(213..2288)
                     /gene="glyS"
                     /locus_tag="ACG8M9_001623"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0378398.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2290..3207)
                     /gene="glyQ"
                     /locus_tag="ACG8M9_001624"
     CDS             complement(2290..3207)
                     /gene="glyQ"
                     /locus_tag="ACG8M9_001624"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0378399.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3593..4411)
                     /gene="recO"
                     /locus_tag="ACG8M9_001625"
     CDS             complement(3593..4411)
                     /gene="recO"
                     /locus_tag="ACG8M9_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0378400.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4554..5453)
                     /gene="era"
                     /locus_tag="ACG8M9_001626"
     CDS             complement(4554..5453)
                     /gene="era"
                     /locus_tag="ACG8M9_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0378401.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5468..5863)
                     /locus_tag="ACG8M9_001627"
     CDS             complement(5468..5863)
                     /locus_tag="ACG8M9_001627"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0378402.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5847..6323)
                     /gene="ybeY"
                     /locus_tag="ACG8M9_001628"
     CDS             complement(5847..6323)
                     /gene="ybeY"
                     /locus_tag="ACG8M9_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0378403.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6352..8544)
                     /locus_tag="ACG8M9_001629"
     CDS             complement(6352..8544)
                     /locus_tag="ACG8M9_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0378404.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8568..9539)
                     /locus_tag="ACG8M9_001630"
     CDS             complement(8568..9539)
                     /locus_tag="ACG8M9_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0378405.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10244..11623
                     /gene="brnQ"
                     /locus_tag="ACG8M9_001631"
     CDS             10244..11623
                     /gene="brnQ"
                     /locus_tag="ACG8M9_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0378406.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3230 a         2523 c         2161 g         3939 t
ORIGIN      
        1 aaattcctaa tttaaaagta aataaaacag taacaagatt ataattaatc gttttcattt
       61 ttatttcctc acttttcatg agcgttttga aaattcgaac aaagaataaa ataacaaaaa
      121 ttattaaaat agaattaata acagctattt ataacaaaaa aaacagagac acagcacgtt
      181 tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg tgatgatttg atccaagctt
      241 gctaatgaca aagccatggc agctatttga cttagttgtg ttagtcggtt cttacgtact
      301 gctggttcat cagccatgac catcgtttga tcaaaataat tttcgatcag tggacgaaga
      361 gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag caaaacttgc tttcattgct
      421 tctactgctt tgtgtaattc tttctctgcg tcgttttcga acagctcagg gtctacttct
      481 gattgttttt caagctgcgc ttttttcgct aagttcacga cacgtgtcaa agcttccatt
      541 gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca attgtgctgc tgcaaatact
      601 tttgtcagat ctttttgttc cgcattcaaa acagcatcga tgacatcatg acgaatattc
      661 ttcgttgtca ataattggcg cagacggcct ttgataaagt cgatcacttg ttgttgccct
      721 tctgataaac caatgccgaa acgatccgta tctttactga taacatcgct aatcgtttct
      781 tgcagaacag acaaaggaaa ggcccatttt ttcgattcga tgatccgaac gacaccgtat
      841 gcttggcgac gtaaggcata aggatcgttt gacccgcttg gaatcatccc aactgcaaag
      901 aaagaaaaga tgctatctaa tttatctgca atcgctaaaa cagaacctac tgtagactga
      961 ggtagctctc cttcactgga tataggcatg taatgctcac gaatcgctgt cgctacagca
     1021 ggtttctcgc ctttcagaag agcatatttt tcgcccatga tcccttgtag ttcaggaaat
     1081 tcccctacca tgttggttac tagatcaaat ttatagattt cagcagctcg ttgcaaatcc
     1141 atcaattctt cctcagatag tcctacttgt tcgccaatga tacgggcaat gacttgtacc
     1201 cgttgcattt tctcatagat actaccgatt ttttcatgga aagtaacagt ttttagtttt
     1261 tcaacgtatt ggtcgattga agctttcttg tcttcattat aaaagaattc cgcatcttct
     1321 aaacgagccg tcaatacttt ttgattgcct cgaaccacat cttccaattt tacccgatca
     1381 ccattacgag cagaaataaa atgaggaagc agcatacctt gatcattccg tacctcgaaa
     1441 taacgttgat gttctttcat agaagtgact agtacttctt caggaacaga aagatatttt
     1501 tcttcgaagt cgccagaaaa gacagttgga tattcgacta aattgttgac ttcttctagt
     1561 aaatcttcgt ctagatcaat aacccagtga tttttttctg ctaattcgtt tgcttgtgta
     1621 acgatcattt cttgtctttt ttgcggagac gcaatgacga attgttcatt caatttttct
     1681 tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta agaaacgatg acctcgagag
     1741 gctctgcctg ttttgacatc caagatttcg aatgggatta cttcgtcatc taacaatgcc
     1801 acgatccagt gaatagggcg gatatattca aaatcgtatt ctgcccaatg catcgttaca
     1861 gggaaagtca aacttttgat cacccctgct aagcctgtca atacttcttt tgcttcctta
     1921 ccatgagtgt atttcgttac atagacatat tccacacctt ttaattctct aaaagtgata
     1981 gcgtctgtcg tcagaccttg tccgcggaca aatccttccg ctgctttcgt ccagtttcca
     2041 tctgcgtcca aagcaatttt tttggctggt cctttgactt cttcttcact gtccgcttgt
     2101 cgttctggaa tatttgttac tttgattgct aaacggcgcg gtgtggaaaa tgtttcgatc
     2161 gtatcataag tcaattggtt ttcatctaaa aattttgctg ttttttcttc taattgtttc
     2221 atgcttggtg tgacaacatg ggcaggcatt tcttccagcc cgatttcaag taataagtct
     2281 tttgccatct tattcagcct ccttcacggt ttctggattt gctgtgtttt tattcaacaa
     2341 agggaatcct aatttttcgc gttctgccac aaagattttg gctacagcac gagccatgtt
     2401 gcggatgcga gaaagataac cggcacgctc tgttacggat actgcaccgc gggcgtctaa
     2461 taagttgaat gtatggctac atttcaagat ataatcatac gccggatgaa caagattttc
     2521 atcgatacag cgtttagctt ctttttcaaa acgatcaaag ttgcttagta gcaagtcttg
     2581 gtcgcttact tcaaaagaat atttggaatg ttcatattct ggttgtttga agatctcgcc
     2641 atatttcacg ccatcagccc attctaaatc atagacactt tcaacttctt ggatataaga
     2701 agcaagacgt tctaatccat aagtgatctc agaagttact ggtttacaag gcagtccacc
     2761 aacttgctgg aagtacgtga attgtgtgat ttccattccg tcaagccata cttcccaacc
     2821 cacaccggca cagcccatgg atgggttctc ccagttgtct tcaacaaaac ggatgtcgtg
     2881 ttctaatgga tcgattccta aaaggcgcaa gctttctaaa taaagctctt gaatattctc
     2941 aggtgaaggt ttcatcacca cttggaattg atgatgctga tacaaacggt ttgggttttc
     3001 accataacgt ccatcagctg gtcggcgaga tggctctacg taagcagcat tccatggttc
     3061 tggtccgatt gcccgtaaaa aggtatatgg gctcatcgta cctgctcctt tttctgtgtc
     3121 ataagcttgc atcagcatgc agccgtttga tgaccaaaat ttttgtaatg ttaaaatcat
     3181 atcttggacg gtcaattttt tactcatctg ttttgcccct ttcaatttta aagcgttgct
     3241 gtcaaataag aatacaggaa agaaaacgac aaaaaatccc tatgtctgat tacagacata
     3301 gggacgaata attcgcggtt ccaccctact tccgaataaa gattcggcag cttcatttat
     3361 cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg ctcccactct cccgaactcg
     3421 ctaaaccacg tgttttttac tgatcttggt caatgtacgt attcattttg cagttttctt
     3481 gtttctataa tagatcctat cactgaacga tctatttaat ctatggttaa taatagacgg
     3541 aactccctgt gtttgtcaaa tggttttgat tttttgttgt atgttttatt tactattctt
     3601 ctttttcagg tgtctctttt tctttttcag acatgtcttt ctctgacgtc tttaatacgt
     3661 gtgcccaaga cttcatttga ttgatgaact ttttgctttt caaatggata ccgacatatt
     3721 catcatacag ctggtcgatg agttcacgga ttgcttgttt ggtttcttct ttcagatgga
     3781 ttccactaat tttatcataa gaaatagctg aaaacatccg aacgaaataa acagctcgag
     3841 gatctgcatg ataacggtga tcgtccatcg accaatgtct ttcgcatatc acaccaccat
     3901 atttggaaga atagtcaaat tttccttttg tttccccgca tacgacgcag tgtgtccaga
     3961 ttggtgcaat tccgaagcgt tgcagtattt ggacttcaaa aatattcgtg atgatttctg
     4021 catcgtctcc ttggtccatc cgttgcagtg cttgttgcgt aaactggaac aaatgcgggt
     4081 cgtactgatg atcttcgatt gccgcatcta ctagattcaa taaatatgtg ccatagccgc
     4141 tcaaaaagat gtcttcttgg atgtgtcgaa acgcatggac atctttgaca ctgcttaaaa
     4201 atgacaaacc ttcacttcga aaattcccga tataaactgc ttcagtaaaa gggagcaaag
     4261 ccggagctaa gggctgattt tgccgatgga tctctttgat ataaaacatc attttgccag
     4321 aagattctgt aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa
     4381 tcaagccttt tgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa
     4441 aagcagattc tgccgatcca gatcatttat gttgcgatct tccgcagctt ttctcaacac
     4501 gcggattttt attcagttta tttctatacc taatttacaa gaagctcaga atatcagtat
     4561 tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt tgtctcgcca gtctttttga
     4621 actttgaccc aaagttctag aaagactttg tctcctaaaa gattttcgat atcttttctt
     4681 gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat ccctttttgg
     4741 ctatctcttt cgacaataat cgtagcctgg atatggattt tatcattctc gtttcgcttc
     4801 atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact
     4861 ttctcacgga tcaattcaga aacgatgaag tattcaggat gatcagtcac ttgatcatct
     4921 ggaaaatatt gtgggccctc tggcatttca ctgactaata catccatcaa acgttcgacg
     4981 ttgtttcctt ctgtagcaga gattggaatc acttgtgcaa aaggcatctg cgttgaataa
     5041 tcttcgatga ttcccaacaa ctcgtctgga tggattttgt cgattttatt gataatcaga
     5101 taaacaggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccgaccgcgt
     5161 ttttgatcag cactaatcat aaataagacc gcgtccactt ctttcaacgc actgtaagct
     5221 gcttctacca taaaatctcc taaacgatgc tttggtttat ggattcctgg tgtatcaata
     5281 aaaatcaact gagcttctgg tgtagtgtat actccttgga ttttatttct agttgtctgt
     5341 gccttgtcac tcatgatggc aattttttga cccacgattc gattcaataa tgtggatttc
     5401 cctacatttg ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatatgtt
     5461 ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc aacccaacga
     5521 tgacggtaaa acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc
     5581 caatcggatg aaaatgaaaa tccgtaaaca tgtccacgac attttcaaaa acagtattga
     5641 tgatctctac tatccatacg agaaaaacag ctaaaagcag ccataaccat tctatccgat
     5701 caagttggaa aatcacgccc ataaacaaag cgaggattcc gaacgcaaca tgtttcctca
     5761 tgttgcgttc ttcctcaaaa acagttttga ctccttggac ggcaaattcc acggacatca
     5821 ggaaatgttt gtttttctct acgtttttat cttttaagtc cataggcatc taagatctct
     5881 ttctgtaaac caaacatttc tttctcatcc tctggtgtca tatggtcata cccgttgata
     5941 tgcaagaatc catgaaccgc taaaaagcca agttcccgat catacgaatg gccgtaagct
     6001 tctgcttgct ctgcagcacg ttcagtagag atcatgatgt ctcccagtac acgaggcaat
     6061 ggattctctc catcttcaaa aatcactggg atttcgtctt cgccctcttc ttccaaggca
     6121 aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt
     6181 gctgcattgt ccatgaaagt aacagacatt tctgtttctt ctggcaactc taaatatgat
     6241 gctgcaaatt gcagcaagtc gtcgatttct ttcattttct cttctgagac ttggtttgtt
     6301 tcatccatga aggtaatatc cattatacat cgcttctttc tattcttctt gctattcttc
     6361 ttgttcttct tttatcttct caacttccga tttcatttta ggatatttga tccgagaatg
     6421 gaaggtactg cttaaaccgc tgatcaatga tttttctact ttacggattt cttttaacgt
     6481 caatccactg tcgtccaatt gtccatcgga aatacgctct tcaataagat tatggacaaa
     6541 actttcgatt ttttcgctcg ttggatggtc cattgcccgt actgccgctt cacaactgtc
     6601 agctatattg acgattcctg cttcacggct ttgaggtttt ggtccaggat aacggaattg
     6661 ctcttccgta acttccgggt tgcgttcctt tgccttaacg tagaaataac gcatcagcgt
     6721 ggttccatga tgctgtctac aaatatcgat caccatttgc ggcatattat aatcttccaa
     6781 tattttggca ccatctgtca catgtccaaa gataatatgt ttactgtctt ctggcaataa
     6841 gaaattgtgc ggattttccg ctcctgaagg caagttctca acgaaaaagc tggcgtgttt
     6901 gatcttgccg atatcatgat aatagcaagc tactcgagtt agtaaagaac gtccaccgat
     6961 ttctgctaca gcattagcac ttagattggc taccatcatg ctatggtgat acgtacctgg
     7021 tgcttcttcc agcaactgtt tcaataaagg atgattggga ttgctgagtt catttaatac
     7081 gatcacacta tcatcattga ccatcaattc aatatagggg tgcagaccta tcgtcagcaa
     7141 gaatgaaaac agtgcacctg tgaatccgca gactagcgtt aaccaagtcg ttgcatcccc
     7201 aaagctcatt ccttggtaga taatcaaaat gacatccatc atcacaggaa aaataatgac
     7261 ccacatagca gcggataacc cttgttctga gatccgtttg cgtctgacga tcgttgccat
     7321 gagaccagaa aatacatagg acatcaaaat aactgtcaga aagttcgtgc caatcgaatc
     7381 ataaaaaatg aaaagtgcgg atactgcttg aaaaatacca gccatgatcc ctgctcttcg
     7441 attcaagaaa aagcttaaaa caagtggagc aaaagccgcg gggtaaaaca gcggaatata
     7501 agcagcttgt tctgtttgga agagttggaa aaatttcatt agtacgacgc ttactgacat
     7561 tgccgtcacg tagaaaagta cgtattttgt tctttctcct gcattttgga attgaagtga
     7621 attataaatc agtacaccga tttgaagcaa aacagctaaa accatggcaa tgagtgggaa
     7681 aatagaagtc gtttgattcg tcaatcctag taattccaat tttttcatcg ctgaagcatc
     7741 aatttgattt ccttcgcgaa caataatttc tccttggaag atcatcactg gttgtacagc
     7801 ttctcgtgca gattgtttta attcttctgt ctttttctca ttcaatacat cattaactac
     7861 gatgccttga tccactaagt agcgaatcat ttgttgctgc tctgttgtga cattcaaata
     7921 ttgaatctga tcttctgctt cttgtttaaa agcttccaat tcgctttcac gaacttgttt
     7981 actcatctgt tcgtcaatga gcttcaaact ttcatcccta actgtgtgaa tttccgtttc
     8041 agtcatttca aatatcgtcg aataaaaatt gttcggcagt ttttggtaaa acgcaacgtt
     8101 ttctgcattc acgttttcaa aatttttctt cagtgtagca atccgctctt ctgtagtagg
     8161 tttcgggata ttttcatctt ccttcgcctt atctaccttg ccattataag atttgttgat
     8221 ggtatcattc gtcttatcga tcaattcgaa taattgcttg atccgattgt tctggtcatc
     8281 tgccagatct ttttgataag tatattctgg agtcactgct tctgctgcca gttttcttct
     8341 ttgttcggtt tcttccgtgt tctcaatcgt tttattcgca cggatactct cttctgctac
     8401 ttggccttct ctgaaatcaa tggccttttg tttgacacta ctaaatgtaa cgccgcagac
     8461 aatgataaaa aacaagaaca gaaggaaagg caatgctgcc tttccgagtt tttcttgtag
     8521 tttctgcagt gttttattca acatgtgctt atcctcctaa gcaaagctta ttcttgatga
     8581 agatccgcct tttcataagc tttgatgatt tcggcaacta cagggtgccg aacaacgtca
     8641 cttgcttcaa aattaacaaa atcgattttt ttgatttgct ttaatgttcg ttctgcatga
     8701 acaagaccac ttgtcgtccc acgaggcaag tctatctgac ttgtatcccc attgacaatc
     8761 atttttgatt gataaccgag acgggtcaag aacattttca tctgagcgac tgtcgtgttt
     8821 tgtgcttcgt ccaagataac gaatgcatca tccaatgtcc gcccccgcat gtaagctaaa
     8881 ggtgcgattt cgatcacgcc tcgttccatc aatcggttcg tatgatccaa tccaaaaatt
     8941 tggtataaag catcataaac aggccgcaga taaggatcca ctttttcttt cagatccccg
     9001 ggtagaaagc ctaggttttc ccctgcctcc acagcgggcc gcgtcaaaat gattttttgc
     9061 acttcgcctt tttttagggc agcaattgct aatactacgg caagaaacgt tttccctgtt
     9121 ccggctggcc ctacaccaaa tacgatgtca tgtttggcta ccgattcaac gtatttcttt
     9181 tgaccgctgt tttttacccg tataggtttt ccattacggt ctttgacgat ctcttcctca
     9241 tacatctcaa caaaataatc taatgtacct ttttgcccca ttttcaatgc cgtcacaaca
     9301 tctggcgtac ttacgtggat tcctcgtttg atcaattcct gtaatgtccg aatgactgag
     9361 gcagccaagg aaatctggga ttgctcacca atgatctgaa tgatttctcc acgtgtatgg
     9421 atggttgttt ccgttgtttc ctcgatcagt tttatatgct tgtcgtgtgc acctaaaagc
     9481 atgctgacat catcagcagc tgtcaatcta atctctaaag aactagactc ttccgtcaaa
     9541 aatagacatc cccttttttt cgattcattc tttcctattt taccatactc agaagaaact
     9601 atatagcaaa taagaaaagg ttgtaaaaga gtcgcttaac catacgtacc aagaaacgtt
     9661 aaaaaatagc cgtttccatt cgctaaaggt ctcgttttga taaaaagaag ctgagaatgt
     9721 aagttatatg aaagtggaga acaaaatatt tcgtttcatt caacaagtat tctctaggta
     9781 tttttcctgt ttcaaaaaaa gaaaacaaaa tagttaaaat attcttgcat ttcaaaaaaa
     9841 acaggtgtat tattacgtca acaaataaac atacagcaaa gcgtcgagga aaacagtagg
     9901 ctattcaacg atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag
     9961 acgaacatca tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc
    10021 aggctagcgg ataactttat caaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa
    10081 aaaggtggta ccgttctaga atctagagcc cttttcccaa gtggaaaagg gctttttttt
    10141 attttaaggt ggtggttttc atggaatctg atattagtgg cattactaat tgctgcaacg
    10201 tttatatcgt caatcaaaat gataattatt ggaggaaaaa tttatggaag aaacaaaaac
    10261 ttattcatgg aaacaactat tgatggtcag ttcacttatc ttcgggatgt tcttcgggtc
    10321 aggtaatcta atctttcctg tccacttagg tcaaatggct ggcgcaaact gggtcagtgc
    10381 tggcgtagga tttgctatct caggtgctct tttcccatta ctagctatct tagctgtagt
    10441 agtgacaaaa agtgatggtc tgtatgactt agctaaacct gtcggaaaat ggtatgctgc
    10501 cttgttctta atccttgttc atttgacgat cggaccattt ttcggtacac cacggacagc
    10561 tgctactgct tttgaaatgg ctgctaaacc atttttgcct gaaaaatata attcattagg
    10621 tatgttttta ttctctgctc ttttcttttt acttgcttat ttccttactg tccatccaac
    10681 acggttggtc aaatacgtcg gaaagtatct aaactccttg tttcttgcat tattagcaat
    10741 catttttgtc gtttctttat tccatccaat gggaaatcta aatcaatctg ctgcagatac
    10801 ttatcaaaca aacgctttaa caaatggtgt cctagaaggt tataacacgg ttgatgcagt
    10861 agctttgctt gccttaagtg tcacttttgt ccatgcagtc cgtgaccttg gtttcaaaga
    10921 taaaaaagtc acagaattaa cggcaaaagc tggtagcctg agcgttgcgc tagaagtcat
    10981 gatctatttt ggacttgttc ttctcggtgc gatgagtttg aataagatgt ctttatcaga
    11041 aaatggcgga actgcacttt ctcagatcac taatttttat ttaggtaact tcggcgctag
    11101 cttcttagga gtgatggtca cattaggtgt cttcactacc gctatgggac tcgttgtttc
    11161 ttttgcgcaa gatttccata aactgttccc taaagtcagt tatatgacat ggctgcggtt
    11221 aacgactttc gtctcttttg tcgttgcaaa tgctggttta gacaatatca tccaatggtc
    11281 tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg ttgatcttgc tttccttaac
    11341 agcaaaattt ttccaaaaga caccatttgt ctatcaagta acgatgttgt ttgctgctgt
    11401 tcctgccgta ttggatatgc tggcaaattc gcctgcttta gtgagtcaac aaagagttgt
    11461 agcaagcatg ctcgaatttt atcatcatca cgttcctttt gctgcattag gacttggctg
    11521 gatggtccca acgttacttg gttacgctgg cagcctactg ttttattacg cctatcgcct
    11581 atcagggtat aaacaagaag caaatgaact accagaagaa taaccaaatc aaacacaatt
    11641 taacataaga aatattaaac aggaaaaaac cagctagatg aaagaatcct attctttcgt
    11701 ctagctattt tttagttctc ttttttacgc gcacaaaaaa actgcggaca atgattgtcc
    11761 gcagtttaaa tgggctctat aatatctagt gttggtgacg ccttctgttg ccaatacttt
    11821 ttcttttttc taataaacgc aaacagacac ctt
//
LOCUS       DAZKNM010000075        11802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000075 DAZKNM010000000
VERSION     DAZKNM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.076"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..661
                     /gene="plsY"
                     /locus_tag="ACG8M9_001632"
     CDS             23..661
                     /gene="plsY"
                     /locus_tag="ACG8M9_001632"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0378407.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(793..1668)
                     /locus_tag="ACG8M9_001633"
     CDS             complement(793..1668)
                     /locus_tag="ACG8M9_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0378408.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1750..2541)
                     /gene="codY"
                     /locus_tag="ACG8M9_001634"
     CDS             complement(1750..2541)
                     /gene="codY"
                     /locus_tag="ACG8M9_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0378409.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(2559..3959)
                     /gene="hslU"
                     /locus_tag="ACG8M9_001635"
     CDS             complement(2559..3959)
                     /gene="hslU"
                     /locus_tag="ACG8M9_001635"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0378410.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3973..4521)
                     /gene="hslV"
                     /locus_tag="ACG8M9_001636"
     CDS             complement(3973..4521)
                     /gene="hslV"
                     /locus_tag="ACG8M9_001636"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0378411.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(4558..5463)
                     /gene="xerC"
                     /locus_tag="ACG8M9_001637"
     CDS             complement(4558..5463)
                     /gene="xerC"
                     /locus_tag="ACG8M9_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0378412.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(5661..7037)
                     /gene="trmFO"
                     /locus_tag="ACG8M9_001638"
     CDS             complement(5661..7037)
                     /gene="trmFO"
                     /locus_tag="ACG8M9_001638"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0378413.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(7006..9084)
                     /gene="topA"
                     /locus_tag="ACG8M9_001639"
     CDS             complement(7006..9084)
                     /gene="topA"
                     /locus_tag="ACG8M9_001639"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0378414.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(9206..10063)
                     /gene="dprA"
                     /locus_tag="ACG8M9_001640"
     CDS             complement(9206..10063)
                     /gene="dprA"
                     /locus_tag="ACG8M9_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0378415.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(10119..10886)
                     /locus_tag="ACG8M9_001641"
     CDS             complement(10119..10886)
                     /locus_tag="ACG8M9_001641"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0378416.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10879..11739)
                     /gene="ylqF"
                     /locus_tag="ACG8M9_001642"
     CDS             complement(10879..11739)
                     /gene="ylqF"
                     /locus_tag="ACG8M9_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0378417.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         3051 a         2599 c         2086 g         4066 t
ORIGIN      
        1 tcaacttgaa agagggtaaa aaatgaaatt gatcttatta cttattttag catatctttt
       61 aggttctatt ccttccggtg tctggattgg caaattgttt ttcaaaaaag atatccgaca
      121 acacgggagc ggtaatactg gaacgaccaa tacatttcgt gtattaggca aaacagccgg
      181 gattgttgtc ctgcttatgg atattttaaa aggtacattg gctacttgtt tgccgatgat
      241 cttccacctt tctgggatta accctctttg gtttggggta tgcgccattc ttggccatac
      301 atttccgatt tttgctgggt tcaaaggagg aaaagctgtt gcgacaagtg caggaatgct
      361 tttaggattt aatcctgtat tttttgttta ttcttgtatc atctttatcg ttagtttatt
      421 ttgtacaagt atggtgagtt tgagcagtat gatcagtgca gtattgatca ctttatctac
      481 gatcgtcctt ccttatgtag ctcctgtgat tttggctaag cccaattggt tactgacgat
      541 cattgccttt ttagtttctt cctttatctt ttatcggcat aaagacaata tcaagcgtat
      601 cagaaaccat actgaaagtc gaatcccgtt tggtttgaat agcagcaaga aaaaatcctg
      661 atactttttc attccaaaga gataaaacgg gagtgtgaca aatcgatgtc acactccctt
      721 attacttaca ggatagcttt tttacacacc ttggtaaacg gtgttccctg ctaacttctc
      781 ggtcacaacc tcttacttca ctgtgatgga atattttgtc ttgaagattt catgggcacc
      841 tagttgattg atccctactt tttccatcaa gtttccacta ctgttcaccg tgtcagcgat
      901 tccgcaccac ggttcgatac aaacgaaagg cgcttctttt ggataaggag accagatccc
      961 tacataaggc atatctttat agcttaaagt tacgctatgg gaagattttt cacttcgaat
     1021 ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca tcttgctcaa aaagttcacg
     1081 attcaaagcc agattcgtat ttgtctgacc taacgtcttt tgatccatat cgatgaatgg
     1141 gcctttcaat ggtaggcgta atcttgattt caatggagaa aatgccagat agaaatcttc
     1201 aaatgacaat ccttcttcaa gaggtacatt gaaagcagga tgtccaccga tagaaaagaa
     1261 catttctttt tcaccggtat ttgccacttg ataatgaacc gtgatgccgt ctcctcctaa
     1321 ttcatagctg atcactaatt caaaatcaaa tggatacttt tctaatgtct ccggcgtact
     1381 ccttaagatg aaactagcca tttcttgtcc atgttcaatg acttcaaaat ccatatcccg
     1441 agcaaatccg tgttgcccca tttgatatgt tttcccttga taaacatact gatcatcttt
     1501 taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg ccccaatacg ctggatcacc
     1561 ttgccagata tactctaaac catgttcttt tgatttcaga ctgatcaatt ccgcaccgtg
     1621 ttcagcgatt tcggcgatca gctggtcatt ttgaataata acagtcattg ccattcctcc
     1681 aacttcgact tactttttaa aatgggaagg ctggaacaaa catttctatc tgccccagtc
     1741 cccttacttc taataactgt gttttgctaa ctcgtcttta aaacgggaat tcaatacttt
     1801 taaataagtt cccttcatgc ctaacgaacg agattcaatg attccagctg attccaattt
     1861 tcgtaaagca ttgacgatca cagaacgagt gatgccgata ctatcggcaa tattagaagc
     1921 agtcagacga ccttcatctc catccaatgc ttcaaagata gcttgtacag ccttcaattc
     1981 gctataagat aatgtgttga ttgccatttg gacagccgtt gcactccgta catctgcttc
     2041 gatgcttcga gacttctgat aaaggatctg cataccgaca accgtagctc catattctgc
     2101 taagactaga tcgtcatctg caaacgattg ttccatacga gataagataa tcgtacccaa
     2161 acgttcgcca gctccaaaga tcggaacgac agtcgtcaga ccaaatggat atttctctct
     2221 tgactctacc ggaaatgcag taatatcact agtaattggg atattagctt cagttttcat
     2281 caactgtgcc actgtatcag tataagattc aggaaattgt ttctcaacaa acattgtttt
     2341 gattcgttca ttgttcacat cgtgacgttc attataacct agtaatactc cgtcacttcc
     2401 tatgatatag gtgttgctgt caagaatacc acctagtgtg acagccattt tatcgtaagg
     2461 caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa agtgtgttta tcttgcgggt
     2521 tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt ataaaatata gcgacttaaa
     2581 tcttcatttg ccgcaattga gccaagtttg tcatccacat atgcttctgt gatcgttatt
     2641 tctcccattt gcatatctgg tgcttcaaat aatagatctt ctagtagttt ttccaaaatg
     2701 gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt tgacatcgaa agcgatttga
     2761 gcgatccgtt caattgcttc ttttgtaaac gtaactgaca cattttctgt accgatcaac
     2821 gcgacgtatt gttttactaa agcattattt ggttcggtca gaatcttcac aaaatcttca
     2881 gctgtcaaat catctaattc tacccgtatc gggaagcgtc cttgaagttc tggaattaga
     2941 tcacttggtt tagacaaatg gaaagctcct gaagcaatga ataggacgtg atctgtttgg
     3001 atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa ttggcaatat gtctctttgg
     3061 acgccttctc tggatacttc tccagattgc tgtgatttag aagtgatctt atcgatttca
     3121 tcgataaaaa taattccact ttcttccgct aatttgattg cctcactatg gatatcagca
     3181 tccttgacta attttgctga ttcttccttg atcaataact ctaaagcttc tttgactgtt
     3241 accgtccgtt ccactttttt cttcggtgtc aaagcaccta gagtttcatt taggtcgatc
     3301 cccatttgct ccaatccatt gttcatcatt ggtgcaggtt tcttttggtc ttcaacttca
     3361 atggttactt cacgactatc cagtaaaccc ttttgtagtt gttccaagac tgtacggcga
     3421 ttgattcgaa tatcttctgt cacttcttct tgcggttcag cggtattttg ctgactagca
     3481 ttaaaaagat tcatcatttg ttcaaattga ttagtggatt gtttctgttc ttttttgata
     3541 ccaggtacca agatttttac caaacgtttt tctgcatttt ttttagcact tgtgtataca
     3601 cggctgtact gctctttttc tacgatttgg atcgcatttt ctactaaatc ccgaaccatt
     3661 gattctacat ctcttccgac ataaccgact tcagtaaatt ttgttgcttc gactttgaca
     3721 aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt ctgttttacc gacccctgta
     3781 ggtccgatca tcagcatatt ttttggtgta atatcttgtt gcattttttc atctaactgc
     3841 aaacggcgat agcggttacg caaagcaacg gcaacagatt tttttgctgc gtgctgtcct
     3901 acgatatatt catctaattc acttacgatt tgtcttggtg tttgttttaa ttcatacata
     3961 tctactcacc tcttacagtt cttctacgat aatattatga ttagtaaaga tacagatatc
     4021 tgcggcaata tttaacgcat tttctgcaat ttctttcgca ctcatgtcgt tttgagcata
     4081 gtttttcatt gctcgggctg cagccaacgc aaaattccct cctgatccga tagctaagat
     4141 accatcatct ggtgtgataa cttctcctgt acctgaaaca agaagcattt ctttatcatt
     4201 cattacgatc agcatcgctt caagtttttg catagattgc tgtgtccgcc attcttgagc
     4261 taattcgaca gctgcgcgtt gtagattccc gttatattcg tttagtttgc cttcaaattt
     4321 ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa cctacaacaa cttctccatt
     4381 gtagatacgt cgtactttct ttgcagtgcc tttcatgaca acagattcac ccattgttac
     4441 ttggccatct ccagccatag caaatttccc gtctttttcg acggcacaga tagtggttga
     4501 atgaaatggt gattcgacca tgtttgtttc ctcctaaatt tgttcttttc cttattttta
     4561 cgctcttgga tggaaagaac gatagttttt ttgtaaactt tcttttgtta catgggcata
     4621 gatctgggta gtcgataaat tggcatgtcc tagcaattct tggactgtcc gcaaatctgc
     4681 cccattattt aataaatgag tagcaaatgt atggcgcagc atgtgaggat ggatatcact
     4741 atccaaactg ctttttttga tcatttgatt caagacatat tcaattccag taggcgtgat
     4801 tggttttcca tgatgattca caaaaacgat atcatgctct tcgtgatatt ttgtcatcaa
     4861 tacctggcgg ccttccctga aatattcttg cagagcatct tgagcaaaag aacctagagg
     4921 agcatatctc tctttgttcc ctttaccatg gatcaacatg acctctgaat caaaatcaat
     4981 gtctgatacc gacaaactgc tacactcact taaacggatg cctgacccgt aaaggacttc
     5041 cagtaaagcg cggttcctca aatccaaagg actatctcct gctacgctgt caaaaagtgc
     5101 ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa tttttctttt tgagatgaac
     5161 gtaagaaaat ggattttctt ttatcacttc ttctttgaga agatactgat aaaacgaacg
     5221 taggctggct atcttgcgac tgatagaatt tctgctatag ctaggacgat gatcagctaa
     5281 tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc agcagatcag cgtctccaga
     5341 ttcctccaaa aaatgtttga aatggagaat gtcttcttca tatgcttctt tcgttttttc
     5401 tgaatatcct ctttcgacaa tcaaataacg caaaaatttt tgttcccacg tggcttctgt
     5461 cattttctca ccttcctttt gtcccagaga ataatttagc acagcgttta taaaaagaca
     5521 aataaattgt cagaatttaa ccaatatttc taagtttgtt cacaattggc gcaattcctc
     5581 acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa agagcgagac tatccgtctc
     5641 accctcttca tggagtttcc ttactcattt gggattgcag ttttttctaa ttctgttttt
     5701 attgcctcat tagcttttaa agctcgttgt gcaattgctt catatcttgc ttttttgtct
     5761 cgaatgcgct ctggcagttc aggaaacaga ccatagttgg cattcatcgg ttggaaatgt
     5821 tttccttccg catgagtaat gtagtaagcc atacttccaa tagctgtttc ttgtggaaac
     5881 tcgactggtt ccatttcttt tgctaagcgt gcggcgttga ttccagcaag tagtccgctc
     5941 gctgcacttt caacatagcc ttctacacca gtcatctgac ctgcaaagaa cagatcttcg
     6001 cgtttcgttg attggtaagt cggtttcagc aattctggcg aattcataaa gctgtttcgg
     6061 tgcatcacac catagcgaac gaattcagca ttttctaacc caggaatcat ccggaaaaca
     6121 cgtttttgct cgccccattt caagtgagtc tgaaagccca cgatgttgta taaagaagct
     6181 gcagcattgt cttgacgcag ctggataaca gcataaggac gtttgccagt tttaggatct
     6241 tccaatccaa ctggtttcat aggtccgaaa agcatcgttt tgaatcctcg ctgtgccatt
     6301 acttcaattg gcatacatcc ttcaaaatat ttttctttct caaaagattt aagttctgcc
     6361 acttcggcat tgattagtgc ctcatggaaa gctttgaatt cctcttctgt catcggacag
     6421 ttcaagtaag ctgcttctcc tttgtcatag cgggacttca aatatacttt atccatatcg
     6481 atcgtagctt tgtctacgat tggtgcagct gcatcataga aataaaaacc atctgaacca
     6541 ttgaatgctt tgatgctttc tgctaatgga tgagaagtca aaggtcctgt tgctactacg
     6601 gtgattcctt ccggtatttc agtaatttct tcgtttatca ctgttactaa tggatgctct
     6661 ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt caacagctaa ggcaccacca
     6721 gctggcactg ctgtttcatc tgccgaagtg attactaccg aattcaatcg gcgcatttct
     6781 tctttcaata accctactgc attagccaaa ctgtttcctc ttaatgaatt ggaacatact
     6841 agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt tgactggacg catttcatat
     6901 aaacggacag ggactcctgc ttgtgcgatc tgccaagcag cttcacttcc ggcaagccct
     6961 gctccaacaa catttaccat tttcaatgaa agaacctctt ttctattatt tttggatgtc
     7021 ttcttcgtaa tctccattta cacagactac ttgtttaccg cctttgactt tcttctcgac
     7081 cagatatccg tcacattttg gacaatttct gcccactggt ttatcccaag aagtgaattc
     7141 acattctggg aaacgggaac aaccatagaa tagtctgttt ttcttagatt ttcgttcaat
     7201 gacttgtcct tcatgacata ctgggcaagt cacaccgatt tcttttacga ttggtttcgt
     7261 attccgacaa tcaggaaaat tactgcaagc ataaaacttc ccatatttcc ctaattttat
     7321 caccatcgga tgtccacaga catcacaatc gaatcctgct ggttcatctt tgatttgaat
     7381 tttttcgatt ttttcttccg cattcgtcag ttctttttca aacggtttat agaaacgatc
     7441 aaccactttg acccacgctt ccgtgccttc ttcaatttta tccaaatctc cttccatttc
     7501 tgctgtgaaa tggatatcga cgatttgagg gaagaattca cagattaatg aattgacaat
     7561 ttctcctagt tcagtcggct caaatctttt ttgtgtcaat tttacatagt agcgtctttg
     7621 gatcgtatca agtgttggtg cataagtgga aggtcgccct accccgtttt cttctagtgc
     7681 acgaatcaac gtagcctcac taaaacgtgc tggtggttga gtaaaatgtt gttttggttc
     7741 aatatcaaca gattggacta catcgccttc ttcaagttct ggaagaatat tttctttctc
     7801 ttctttgccg tcatctctac cttccacata cacttgcatg aatcctttga atttgatttt
     7861 tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat gtcactttca ttgtatctaa
     7921 aatagctggt gtcatctgac tagccacaaa acgtgaccag atcaatgtat aaagtttcaa
     7981 ttgatcttta tctaaatatt gtttgagttc atctggtgta cgaagcacac tcgttggacg
     8041 cacagcttca tgggcatctt gtgcgccttg agcatttttg atctttcggt gatcatgtct
     8101 agaaaattct tttccatacg tatcttcaat aaatgctgcg gcctctgctt ttgctgtatc
     8161 ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag ccgactgttc cttgtttacc
     8221 caaagtgatt ccttcatata attgctgtgc taccatcatg gtttttcttg ttcgaaagtt
     8281 taacttacgg gcagcttctt gttgcaggct acttgtagtg aaaggattcg cagggtttct
     8341 tttgcgctct ttcttttcta cttttttaac ttcgaagtct gttccatcaa ttctagcagt
     8401 gacttctttt acactttgtg catctggtaa tttctttttc ttaccgtcga ttccccagaa
     8461 atttgctttg aatttcttgc gagctttttt aaaattaccg tcgatgctcc aatattcttc
     8521 tggaacaaat tctcggatct ctttttcacg gtcaataata atttttaaag cgacagattg
     8581 gacacgtcct gcacttaacc ctttcttgac cttacgccag agaataggac tgatcgaata
     8641 accaacgatt ctgtctaagg tacgacgagc ttgctgtgca tctactaaat ctacatcgat
     8701 cgttcttggt tccttaaaag ctgctttgac tgcgtctttt gtgatttcat tgaagaccac
     8761 ccgatttttt tctttcagat ccaacccaag taggtacgct aaatgccaag caatcgcttc
     8821 tccttctcta tccgggtcac ttgccaagta aactttttga gctttttttg ctgcggcttt
     8881 caggcttttg atgacatcgc cttttccgcg tatagaaata taatggggtt cataattgtt
     8941 ttcaaaatcg atacccattt tacttttcgg taaatctctt atatggccaa cactggccat
     9001 tactttatag tttcgtccaa gatatttctc aattgtcttg gcttttgcag gtgattctac
     9061 aatcactaaa tatttatacg ccattactga atggactcct ttccattgtc gtgttcctac
     9121 tattaaaaga ggattcatac aaatcgtagc taatcatatc cattcagaaa acgtttgtca
     9181 agaaaagcaa atgaaaagta tggtgttatt ctttaaattc tgccaagata tcttcacccg
     9241 aaaccgtgca aactgcaccg tcttggatca aatgatgaca tccggtagaa tgttctgtta
     9301 aaatatttcc tggaacagaa aaaactgttc ttccaagatc caatgcttgc tgtgctgtta
     9361 tcagagagcc gctttttttc ttggcttcaa agacacatgt tcctttagac aaagctgcga
     9421 taatccgatt tctcattgga aaacaatgct ttttcgggcc ggattgagga ggatattcac
     9481 tcaaaagaag atgatcattt gcaatcacct cttgcaagtc acgattttca gcaggataga
     9541 cttgctctat ccctgttcca atgactgcga ttgtccgtcc accatgagag attgctgctt
     9601 ggtgaccgta tgtatctatc cctttcgcgc agccactgac gatcacatat ccagcttcta
     9661 tcactggttt tagtaaataa taaactgttt ttttggctaa agcagaagtg tgtcttccac
     9721 caacaacgct taacatgtta caagaaagta aagatagatc accattataa aacagtacaa
     9781 ttggcggata ggtcaagtga agcagctgag ggggatattt gggatcaaag caagtgataa
     9841 acgactgttt cgattggatt ttgccccagt tagcgtgaat ttcctgccaa cttttgcgaa
     9901 acagctcccg gtgtttggtt attttaccta tcttaatgat gtcttcaaat ccgatttctt
     9961 tataaggata ctgcatcatc cagttagcaa gtgcccattt tccttgacta ctgattcctt
    10021 ttgtataagc aagtttcaat aaattttctt ctatttgata catcataaaa ccgcctttcg
    10081 tctctctttc taataagata cgtaaaagcg gctgtttttt attgtaaata tttttttaca
    10141 ggtgcaaaac ttttacgatg gattggcgta attccttgtc gttcaagtcc ttctaagtgt
    10201 tcctttgttc cataacctgc attatgcgag aaaccatatc caggataaag cacgtcatat
    10261 tctttcatca attggtctcg atatacttta gcaatgatac ttgcagcagc aatcgacaca
    10321 gatcgtgcat cccctttgat tattttttcc tgctgaatag gcaaatccaa ggtcatcgca
    10381 tcgatcaaca aatagcttgg tacaatggac aatttttcta ctgcttggat catcgctaat
    10441 ttacttgctt cataaatatt cacatcatcg attgtctgct gatcaacgat cccgatcccg
    10501 attgccaggg ctttttcttg aacttgttga taaagaagtt ctcgcttttt ctctgataat
    10561 tgtttggagt cattcagccc taatatgatc tcatttttcg gcaagataac tgcagctgcc
    10621 acgacaggtc cagctaatgg tcctcttcct acttcatcaa tgccggcaat ccattggtga
    10681 ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt catatttttc cttaattaat
    10741 gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt gctgcacgcc tttgcgtgga
    10801 tcatttctta atgattctag tgtaggatca tctattctct gtatgctttt caatcgttct
    10861 ttgatacttt ggatggattc agtcatcact tttttgctcc atttcttcgc aacgatccag
    10921 tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg actaattcgc tgccacgatc
    10981 atagtcttct ttatacccac gttttgctgt aatatccatc agtaattccg gtgccaattt
    11041 ttcttcttgc tcgattgata aaccataacg atcgctgatt cttcctgaat aatatcgggc
    11101 aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg acaagctggt ctttgatcgc
    11161 gcctgtcagc gctagttttt tgccgatttc ttcatcttca aatttgggcc ataaaatccc
    11221 tggtgtgtct aacaattcca actcattacc taaacgaagc cactgctgac cttttgttac
    11281 gccaggctta tttcctgtct gtgcaatttt cttgcctact agtcgattca tcaacgtaga
    11341 tttcccaaca tttggaatac ctaagaccat tgcccgaata gcgcgaggct tgatcccccg
    11401 agcagcatct cttgcccgtt tttctttcaa tgcttcttta gccgcaggta ctagaccttt
    11461 aattccttta ctttcttgtg ccgtgatagc taatgcaaga tggccttgtt tacgaaaata
    11521 ttcttgccac agctttgtct gttgtggatc tgccagatcc gctttattca ataaaacaag
    11581 gcgaggtttt tgctgaatga tctgatctag cattggatta cgagaagata aaggtaatcg
    11641 tgcgtctacc aattcaaata cgatgtccac atatttcatt ttttcagaaa cttctcgtcg
    11701 ggcttttgcc atatgtcccg gaaaccattg gatggtcatt gttctctcca ttcctttcat
    11761 accggaatat tccccggctt tacttgtttt tttctttttc aa
//
LOCUS       DAZKNM010000076        11754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000076 DAZKNM010000000
VERSION     DAZKNM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.077"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8M9_001643"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8M9_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0378418.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8M9_001644"
     CDS             complement(323..892)
                     /locus_tag="ACG8M9_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0378419.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8M9_001645"
     CDS             1078..1980
                     /locus_tag="ACG8M9_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0378420.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8M9_001646"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8M9_001646"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0378421.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8M9_001647"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8M9_001647"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0378422.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8M9_001648"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8M9_001648"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0378423.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8M9_001649"
     CDS             8554..8739
                     /locus_tag="ACG8M9_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0378424.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8M9_001650"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8M9_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0378425.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8M9_001651"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8M9_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0378426.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3481 a         2501 c         2055 g         3717 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atac
//
LOCUS       DAZKNM010000077        11604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000077 DAZKNM010000000
VERSION     DAZKNM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.078"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG8M9_001652"
     CDS             237..1877
                     /locus_tag="ACG8M9_001652"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0378427.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG8M9_001653"
     CDS             2031..3353
                     /locus_tag="ACG8M9_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0378428.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG8M9_001654"
     CDS             complement(3415..3846)
                     /locus_tag="ACG8M9_001654"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378429.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG8M9_001655"
     CDS             complement(3910..4746)
                     /locus_tag="ACG8M9_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0378430.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG8M9_001656"
     CDS             complement(4936..5238)
                     /locus_tag="ACG8M9_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0378431.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8M9_001657"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8M9_001657"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0378432.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG8M9_001658"
     CDS             complement(5692..5958)
                     /locus_tag="ACG8M9_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0378433.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG8M9_001659"
     CDS             complement(6212..6937)
                     /locus_tag="ACG8M9_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0378434.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG8M9_001660"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG8M9_001660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG8M9_001661"
     CDS             7490..8242
                     /locus_tag="ACG8M9_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0378435.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG8M9_001662"
     CDS             8258..8737
                     /locus_tag="ACG8M9_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0378436.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG8M9_001663"
     CDS             complement(8988..10568)
                     /locus_tag="ACG8M9_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0378437.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG8M9_001664"
     CDS             complement(10561..11304)
                     /locus_tag="ACG8M9_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378438.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..>11604)
                     /locus_tag="ACG8M9_001665"
     CDS             complement(11480..>11604)
                     /locus_tag="ACG8M9_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFQ0378439.1"
                     /translation="SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         3525 a         2316 c         2060 g         3703 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatg
//
LOCUS       DAZKNM010000078        11518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000078 DAZKNM010000000
VERSION     DAZKNM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.079"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..928
                     /gene="atpB"
                     /locus_tag="ACG8M9_001666"
     CDS             209..928
                     /gene="atpB"
                     /locus_tag="ACG8M9_001666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0378440.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            981..1196
                     /gene="atpE"
                     /locus_tag="ACG8M9_001667"
     CDS             981..1196
                     /gene="atpE"
                     /locus_tag="ACG8M9_001667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0378441.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            1287..1811
                     /gene="atpF"
                     /locus_tag="ACG8M9_001668"
     CDS             1287..1811
                     /gene="atpF"
                     /locus_tag="ACG8M9_001668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0378442.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            1798..2340
                     /locus_tag="ACG8M9_001669"
     CDS             1798..2340
                     /locus_tag="ACG8M9_001669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0378443.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            2363..3919
                     /gene="atpA"
                     /locus_tag="ACG8M9_001670"
     CDS             2363..3919
                     /gene="atpA"
                     /locus_tag="ACG8M9_001670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0378444.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            3933..4835
                     /locus_tag="ACG8M9_001671"
     CDS             3933..4835
                     /locus_tag="ACG8M9_001671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0378445.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            4858..6264
                     /gene="atpD"
                     /locus_tag="ACG8M9_001672"
     CDS             4858..6264
                     /gene="atpD"
                     /locus_tag="ACG8M9_001672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0378446.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            6278..6700
                     /locus_tag="ACG8M9_001673"
     CDS             6278..6700
                     /locus_tag="ACG8M9_001673"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0378447.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            7169..7399
                     /locus_tag="ACG8M9_001674"
     CDS             7169..7399
                     /locus_tag="ACG8M9_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0378448.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            7496..8797
                     /gene="murA"
                     /locus_tag="ACG8M9_001675"
     CDS             7496..8797
                     /gene="murA"
                     /locus_tag="ACG8M9_001675"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0378449.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            8810..8986
                     /locus_tag="ACG8M9_001676"
     CDS             8810..8986
                     /locus_tag="ACG8M9_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0378450.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            9121..9417
                     /gene="yidD"
                     /locus_tag="ACG8M9_001677"
     CDS             9121..9417
                     /gene="yidD"
                     /locus_tag="ACG8M9_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0378451.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            9481..9864
                     /locus_tag="ACG8M9_001678"
     CDS             9481..9864
                     /locus_tag="ACG8M9_001678"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378452.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            10033..10917
                     /locus_tag="ACG8M9_001679"
     CDS             10033..10917
                     /locus_tag="ACG8M9_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0378453.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            11031..11267
                     /locus_tag="ACG8M9_001680"
     CDS             11031..11267
                     /locus_tag="ACG8M9_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0378454.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            11355..11435
                     /locus_tag="ACG8M9_001681"
     tRNA            11355..11435
                     /locus_tag="ACG8M9_001681"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11389..11391,aa:Tyr,seq:gta)
     gene            11442..11513
                     /locus_tag="ACG8M9_001682"
     tRNA            11442..11513
                     /locus_tag="ACG8M9_001682"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11474..11476,aa:Gln,seq:ttg)
BASE COUNT         3786 a         1982 c         2516 g         3234 t
ORIGIN      
        1 gttttttctt agatcaggag caccgtgcat ggggaaaaat tcgcttcgtt tttgactgaa
       61 ataaaaaagt gtctaatttt catgaaaatg ttttgaattt tctgtttcat ggtggtatga
      121 taccgtgtgg ttagaaaccg attggaaaaa accagatacg cacatgaatt gattttatgt
      181 gaatttcggg gaaaagaggt gaaatggatt ggatgaacgc tcgctaacgt ttcacattgg
      241 accaatttgg tttgatggta cagtttgtat aatggtgtta ctgacctgtt tgattgtttt
      301 cttcttagct tactttttta caagaaatct taagttgaaa ccaacaggca aacaaaatgc
      361 gatagaatgg gtcattgatt ttagtcgagg aatcgtaaca gataatctgc ctagaaaaga
      421 attgagcaat ttccatttgt tggcatttac attgttttta tttgtttttg ttgcaaacaa
      481 cataggttta gtaacaaaaa tcgtcttacc taacgaaaca acactttgga aaagcccgac
      541 agcagacccg tttgttactt taactttggc atttataatg atcttactta ctcacttatt
      601 cggggtgaaa aagttaggct tcaaaggata tttcgtaaat tcgttcttga aaccctatag
      661 ttttatgttt ccgatgaaac tgattgaaga atttacaaac ttattaacac ttgctttacg
      721 tctttatggg aatatttatg cgggggaagt tttgctaacc ttgattgcaa aaatgatgac
      781 aagcttaggc tggttctcat taccattagc aattccatta gagatggtct ggatcgcgtt
      841 ctccttgttt attgggagca tccaagcatt tgtctttgtc actttgtcaa tggtttatat
      901 gagccataag attgaagtag aagaataata ctcggttaaa aaacaaacta ctatttatct
      961 attttaggag gaaaacaatt atgaattata tcgcagcagc aatcgcaatc atgggagcag
     1021 caatcggagc cggttatggt aacggtcaag ttatctctaa aacaatcgaa tcaatggctc
     1081 gccaacctga aatgtctggt caattccgta caacaatgtt tatcggggta gctctagttg
     1141 aagctgttcc tatcttaggg gtagttatcg ccttgattct agtttttgcc gtttaattga
     1201 atcagtatta aggcagaaac aacatcgttt gttactgctt ttttacatgg aaactagaaa
     1261 tactccaaag aaaggaaggc tcgcctatgc tgaatcaatt ggcaattgca gaagttggca
     1321 atgcgatgtt aggtaatatc atcgttgtca gcggctcatt tttgatacta ctggctctct
     1381 tgaagcactt tgcttgggga ccaatcagcg atattttgaa aaaacgtgaa gacaagatcg
     1441 ccaatgattt agattctgca gaacaatctc gcatcaactc agcgaaaatg gaacaagaac
     1501 gcgaacaaca attgttagcc tctcgttctg atgcagctga tatcatcaaa aatgcgaaag
     1561 aaagtggaga attaagccgc caaaatattt tgaaggatgc tcaagaagaa gcagctcgtc
     1621 taaaaagcaa agcccaagct gatatcactg tagaacgtga ttcagcgctg aactctgtaa
     1681 aagacgacgt tgcagaactc tctcttcaaa tcgcggaaaa aatcttgaac aaagaattat
     1741 ctccagaaat gcatgaatca ttaatcaatc aatacattga aggtctaggt tcttcaaatg
     1801 aaactagata aatatacagt aggaagacgt tacgggaaag ctttatttga actggcgatc
     1861 gattcaaatc aagcagaaga aatctatcaa gatctcgtaa gtttacgtca aatctatgaa
     1921 caagtacctg gattagggaa tatgttaagt gatgttcgtc tagaacctca tgaaaaacga
     1981 gaaatcatgg acaagcttgt cagcggttat gatggaatcg ttaaaaactt tttagaagtt
     2041 gtttatgagt ataaccgaat ggatgacctt ttgttcatga ttgaagaata tgaacgtcga
     2101 tatgatgaac atcaagggtt actattggga agtgtcacaa cagcagtccc actttcagac
     2161 gaacaacgaa tacaattaga aaagaatgtt gcaaaaacaa tggactatca atcagttgaa
     2221 ttaaaacaaa tcgttgattc ttctattatt ggtggcgcga ttgttgaagc caaccatcga
     2281 gtaatcgacg gaagcattcg cacgcaatta gaaaaaatgc gtaatcaatt aaacagatag
     2341 tacaaagagg taggtgaatc gaatggccat caaagcagaa gaaattagtg ccttgattaa
     2401 agaacaaatt gaaaattatc aaaacgtgct ttcagtcgaa gaaatcggta ccgtaactta
     2461 tgtaggtgac gggatcgctc gtgcacatgg tttagaaaac gcgatgagtg gtgagttgct
     2521 tgaattttca aacggctcat acggaatggc gcaaaactta gagtcaaatg atgtaggtat
     2581 cattatcctt ggcgatttcg agtccattcg tgaaggagat aaagtaaaac gaacaggtaa
     2641 gatcatggaa gttccagttg gagaggcctt gatcggtcgg gtagtcaatc cgctaggtca
     2701 accaatcgac ggactaggtg aaatcgttac tgacaaagct cgtccagtag aagcgatggc
     2761 accaggtgtt atgcaacgta aatctgttaa cgaaccaatg caaacagggc taaaagcgat
     2821 cgacgccctt gtgccaatcg gacgcggaca acgtgaatta gtcatcggtg accgtaaaac
     2881 agggaaaact tctattgcga ttgatacgat catcaaccaa aaaggtcaag atatgatctg
     2941 tatctatgta gcaatcggac aaaaagattc tacagttcgt acacaagttg aaacattgaa
     3001 aaaatatggc gcaatggatt acacaatcgt tgtgaatgcc ggtgcgtctc aaccagcacc
     3061 attactttat atcgcaccat atgctggtac tgcaatgggt gaagaattca tgtacaacgg
     3121 taaacatgta ttgatcattt ttgatgatct ttcaaaacaa gctgttgctt atcgtgaact
     3181 ttccttgcta cttcgccgtc caccaggtcg tgaagcttat cctggggatg ttttctactt
     3241 gcactcacgt ttattagaac gtgcagcaaa attaagtgat gatctaggcg gcggttcaat
     3301 gactgctttg ccatttgttg aaacacaagc tggagatatc tctgcttata tcccaacaaa
     3361 cgttatctca atcactgacg gacaaatctt cttagaaagc gacttgttct acgcgggtac
     3421 tcgtccagcg gttgatgccg gactatctgt ctctcgtgta gggggttctg cgcaaatcaa
     3481 agcaatgaaa aaagttgctg ggactttacg tctagactta gcaagttatc gtgaattaga
     3541 agcatttaca caattcggtt ctgatttgga tgcggcaaca caagcaaaac taaatcgtgg
     3601 acgccgtaca gtagaaatct tgaaacaaaa actgcatgca ccgctagctg ttgagaaaca
     3661 agtagtgatt ttgtatgcct tgacacatgg attcttggac agtatccctg tagacagtat
     3721 tttagacttc gaacatgaat tattcgaata tctggatacc aatcattcag atatttttga
     3781 aacaatccgt acaacaaaag atcttccaga agaagaaacg ttgaacagtg cgattcagga
     3841 atataaagac atgttcttag ctacaaaagg aaataattcc tctacagaag ataaattgaa
     3901 atccattcaa aatgcatagt gaggtgagat gaatgggtgc ttcattaaat gaaatcaaga
     3961 cacgtatcgc ttcaacgaaa aaaacgagcc aaatcacgcg ggcaatgcag atggtgtctg
     4021 cttctaagtt gacaaaatct gaagcttctt cacagaagtt tcaggtttat gcaacaaaag
     4081 taagagaaat cgttacgcat ttgactgcta ctcagttaaa tgatattgcc tcagaaaatc
     4141 ctagagggga tatcaattac aacagcatgc tgctcagccg tcctgtgaag aaaaccggtt
     4201 atatcgtcat cactgcagat ggcggtctgg tcggtggata taacagttcg atattgaaac
     4261 aaacgatgtc tattttagaa gaagaccacc aatcacccga tgattacgtg atgatcgcaa
     4321 ttggcggaac tggtgcagac ttctttaaag cacgcggtat caacctagca tatgagctgc
     4381 gcaatttatc tgatcagcca agctttgatg aagtccgcaa aatcgtaaat atggcaacaa
     4441 ctatgtacca aaacgaagta ttcgatgaat tatacgtatg ttataaccat catatcaatt
     4501 cgctaactag tcagtttcgt gtagaaaaaa tgctgccaat ctcagactta gatccagaag
     4561 aagcaacgac tttcgaacaa gaatatattt ttgaaccttc aaaagaagaa atcttgtctc
     4621 agttattgcc tcagtatgct gaaagtttga tctacggtgc gatcgttgac gccaaaacag
     4681 cagaacatgc tgctggtatg acagcaatga aaacagcaac tgataatgct gctaatatca
     4741 ttgatgagct gacggtttct tataaccgtg caagacaagg ggctattacc caagaaatta
     4801 ccgaaattgt tgccggagct tcggcactag aatagtattg agattggagg aaaaaagatg
     4861 agttcaggca agattgttca agtaatcggt cccgttgtcg acgtggaatt ttctttagac
     4921 caatccttac cagatatcaa caacgcatta gttgtttata aaaatgacga aaataaatca
     4981 aaagttgttc ttgaagcagc gttagaatta ggggacggag tgatccggac cattgcgatg
     5041 gaatcaacag atggtttaca aagaggaatg gaagtcattg atacaggcaa agcaatctct
     5101 gttcctgtag gtaaagaaac attaggtcgc gtattcaacg tactaggaga tacgatcgat
     5161 ttagaaacac ctttcccaga agatgcggaa agaagcgaaa ttcataaaaa agcaccagcc
     5221 tttgacgaat taagtacaag tacagaaatt ttggaaacag ggatcaaagt tatcgatttg
     5281 cttgccccat atttaaaagg tgggaaagtc ggactattcg gtggtgccgg tgttggtaaa
     5341 accgtactga tccaagaact gatccataat atcgcccaag aacatggtgg tatttctgta
     5401 tttaccggtg taggtgaacg tactcgtgaa ggtaatgact tgtattatga aatgaaagat
     5461 tcaggagtta tcgaaaaaac agccatggtg ttcggacaaa tgaacgaacc accaggtgca
     5521 cgtatgcgtg ttgctttgac tggtttgacg attgcggaat atttccgtga tgtagaaggt
     5581 caagatgtac tgttgtttat cgacaacatt ttccgtttca ctcaagctgg atctgaagta
     5641 tcagccttgt taggacggat gccttctgcg gttggttatc aaccaacatt ggcaacagaa
     5701 atgggtcaat tgcaagaacg tatcacatct acgaaaaaag gttcgatcac atcgatccaa
     5761 gcaatctatg tgccagccga tgactacact gacccggcgc cggcaacagc attcgcccat
     5821 ttggatgcga caaccaactt ggaacgtaaa ttaacagaac aagggattta tccagcggta
     5881 gatccattgg cttcttcttc aagtgcttta gcacctgaaa tcgtgggaga agaacattat
     5941 aaagtagcaa cagaagtcca acacgtattg caacgatatc gtgaattaca agatatcatt
     6001 gctatcttag gtatggatga attatccgat caagaaaaag tgttagtttc tcgtgcccgc
     6061 agagtccaat tcttcttgtc acaaaacttt aacgtggctg aacaatttac tggtcttcca
     6121 ggttcatatg tccctgtaga agaaacagtg aaaggattcc gcgaaatctt ggaaggcaaa
     6181 tacgatgatc ttcctgaaga agctttccga agtgttggcc gaatcgaaga cgtcgtagaa
     6241 aaagcgaaaa cactaggcta ctagaaaggg gtgccccatg gatcaatatt taacagtaaa
     6301 tgtggtaact cctgccggtt tggtctatga tcaccatgca gctatcgtcg ttgcacgtac
     6361 gacagctggt gagcttggga ttttgcctaa acatgcacca atcatcgttc cattgacgat
     6421 tgatgaagtt cgtgtaaaac gaacagattc agatacccac gtcgattgga tcgctgttaa
     6481 tggcggaatc atggaagttc gtgataatgt tgtttcaatc gttgctgata gtgcggaacg
     6541 tgaacgtgat atcgatgtat ctcgtgcgga acgtgcaaaa caacgcgcag aacgccaaat
     6601 cgaagaagca aaagcaaaac aagatacgaa cgaattgaaa cgggcaactg ttgcgttgca
     6661 ccgagcaatc aatcgtatca aagtatctaa gcactcataa aatgagatgc ggatgtttat
     6721 ccgtgtttaa gaacagtaaa aagaggttat gacagaagtg gtcagcttca agacataaga
     6781 aggaatttac gaaaattgct tctcaaattt ttgtgaaatc tcagcttatt tttcgaagaa
     6841 gctacttctg gaacaccgta tatcagaggt tgtgaaatca gcgttttgtc ctgagtatta
     6901 gagaaacaaa tggaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
     6961 cagatgattg aacaccgttt atttggaata agggactgcg acaagacttt tgtctcagtc
     7021 cctttttttg catattttta gcatttattt cctaaaaaat gtaaggggat tcctatattt
     7081 ttaagtatac ttgtttttcc ttttatacat tttatggtat tatcatttta ttagttatta
     7141 taaaacaatc gttagaaagg acgtcattat gcaagtatac ggaattgatg cggtgcttcg
     7201 aatcgtgagt catttggcgt ttatctactt agccttctgg tctttacgtt cgttacgcat
     7261 tgaaactttt ttcagaccaa tgcataatac gcaaattaga atggcaatcg tacttttttc
     7321 aattttttta gggtacactg caagtaattt tttcttagaa gttatcgctt tatgccgaaa
     7381 tcttttcgtt agcctgtaaa ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata
     7441 atagaaaaga ttttgagtaa gtgtatgctc attggaattt tttggaggga acaacatgga
     7501 agagatcatc gtaagaggtg gcaatcaatt aaatggaaca gtaagaattg aaggagctaa
     7561 aaatgctgtt ttgcctattt tagctgctag tttgttagca gaagaaggta ttacgacatt
     7621 agacaacgtt ccgattcttt ctgatgtctt cactatgaat caagtgattc gtcacttaaa
     7681 cgtagatgta gattttgatg aacaaaagaa tcaagtaaca atcgacgctt caagacaatt
     7741 agaaatcgaa gcgccttatg agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg
     7801 tccgctactt gccagaaatg gacatgcaaa agtagctatg cctggtggat gtgcgattgg
     7861 taaacgacca atcgatttgc atttaaaagg atttcaagct ttaggagcaa aaattattca
     7921 aaaaaacggc tatatcgaag caattgcgga tgaattgatc ggtaacacga tttatttaga
     7981 tttccctagt gtgggtgcta ctcaaaatat catgatggct gccgtaaaag caaaaggaac
     8041 aaccatcatt gaaaacgtag ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa
     8101 taaaatgggc gctcaagtct atggagctgg tactgaaaca atgcgtattg aaggcgtgga
     8161 tcatttacat gcagtcaacc actcaatcgt acaagatcgt atcgaagcag gtacctttat
     8221 ggttgctgct gcaatgacac aaggcaatgt gctgattgca gatgctatct ctgaacataa
     8281 ctgcccattg atttctaaat tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg
     8341 agtccgtgtc atcggaccaa aacatatctt acctacagat gtaaaaacga tgcctcatcc
     8401 tggtttccct acagatatgc aagctcaaat gacagctatc caattagttg cagaaggtac
     8461 aagtgtggtg actgaaacag tgtttgaaaa tcgtttccaa catttagaag agatgcgccg
     8521 tatgaacgct catgtcaaaa tcgacggaaa tgtagcaatc atggatggaa accatgaatt
     8581 gcaaggtgca gaagtatatg caacagactt gcgggcagca gcagcattag ttttagctgg
     8641 cttgaaagca aatggaatca cacgtgtgcg taacttgaat tatctagacc gtggatatta
     8701 taatttccat atcaaactac agcaattagg tgcagacgtc gaacgtgtag acatggatca
     8761 aacttctgct gaaaaaacag cacaaacgat tgcttaagga agtggctgaa tgagtaacca
     8821 acgatatatc ttattaactt tgataaagat tttagtggtg atcttattgc ttatccttct
     8881 ttttgtggcc ggaacgatga tcggatatgg tgtcatcggt ggcggcaatc catttaaagt
     8941 attccagcca agtttgtgga ttcatattcg agactttttt cattgataaa taagaatcac
     9001 agtggttcta gtcgatatga agtgagccat gaagcagaac atgataaaag aaaacagaag
     9061 ggaacgttga cgttagtcgc gatccctttt tttagtaaaa gaaaagaagg aatctaattg
     9121 atgaagaaga tattgattta tttagttaga ggatatcagc gctttatttc gcctgcattt
     9181 ccacctagct gccgttatta cccaacctgt tccaattata tgataaaagc aattcaaacc
     9241 catggtgcgc taaaagggac ggtaatgggg actgcacgga tcttacgctg tcatcctttt
     9301 gtcaaaggcg gtatcgatta tgtaccattg acattccgat taacgcgaaa taagaaagat
     9361 aaaaaagaac cagattataa aaaggttgtg aaaaatacgg gtagcactga gcgataaaga
     9421 aaaagaggaa atttattgtc aaagtaacaa ctccgcaact acttatatgg gggacaaaaa
     9481 atgatcgaag aactaaacaa gaaagattta ccatggtcct tgttattaga tgcagatcct
     9541 gataaagaaa aagttcaagt atatgtttcc agagggagtg gctggatatg gaaagaaaaa
     9601 gaaaaaacaa taggtgtact gatctatgtg gcaagggaaa aagaatacga aatcgtcaat
     9661 gtagcagtaa ccccaacgta tcaaggaaag gggattggcg gaaaattact tgagacagct
     9721 tttcagaagc tttcacagct agcttcaaac caaactcgga tcattatacg tactggcact
     9781 actagtagcg ctgcgttaca cctctatcaa aaaatgggct atgtggagat tggtcgagtc
     9841 aaagactatt ttatccatca ttaatctgag cccatttttg aaaatggaga tcaattaagg
     9901 gatcaagtga ttttagcaat tcacttacct aaaaatagct ttaactaata aactttcaat
     9961 tcgagaaaat gctttcaaat gctaaataga tacattacaa tagctttagc aaacaaaaag
    10021 gagaatattt ttatgggaat attagttttt gattttggtg gatcggctgt caaatatggt
    10081 tgttgggatg gcaaagagat taaaggtaag ggacaatttg caacgcctga atcttgggaa
    10141 gaaatgaaaa cgcaattatt tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa
    10201 ggaattggaa tcagctctcc tggagtagtg aataatgaga aacaggtgat tgagggaatc
    10261 agtgcgattc cttatattca tggatttaat attttccgag atttggaaac tctgtttcaa
    10321 ctcccagtaa cgattgaaaa tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt
    10381 gctgcccgtg attatcaaga tgttgctttt gttgtggcag gaactgggat tggcggggcg
    10441 ttgttcacga aaggacaaat aaataaaggg gcacatcttt acggtggaga atttgggttg
    10501 atgttcttag atggcgataa gacattcagt aaacttggta cagcggtaca gatggcttgg
    10561 cgttattgtg aacgcaaagg tctcgataaa aatgcttata ccggaaaaga tgtctttgaa
    10621 ttagcagaaa ctggagatgc aatagcaaaa gaagaagttg agagttttta tacgtatctt
    10681 acaaagggat tattcagtat tcaattttcg tttgatccag aagtgatcgt attaggcggc
    10741 ggggtttctg caaaagaagg gctcattgat gagatcaaaa gccgaatgaa gaagctgaca
    10801 gaacaattcg atctgcatga tttcgaaccg cagattttat tatgtgagta cagaaacgat
    10861 gctaatcttg taggtgcagc agcaaactat ctcgctgtac gtcaaggaga gaattaagag
    10921 ctttagagaa ggtgcagctt gcataaaaga agagaatgaa atgaatagga gatttttacc
    10981 taaggaatgt tttatttgct gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa
    11041 aaaaaaataa agatattgaa gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga
    11101 attatgaagt cactcagtta tttataggga aaaaaatgat tggtgagatc ttagcctatg
    11161 ggccaaaaga atatgaaatt ttctttggtg aagaagattt tggaaaagaa aaaagtcttg
    11221 aaaatgcgat agaaacagtg attcgacact ggaatctgca cgaataaaaa aatatcaaaa
    11281 aaactttcaa taaagggttg caaaaatctg gaaaattagg tattataact aagtcaggtt
    11341 gatacaaacc tttcggagga gtagcgaagt ggctaaacgc gacggactgt aaatccgttc
    11401 cttagggttc agtggttcga atccactctc ctccatttca ttggggtata gccaagcggt
    11461 aaggcaaggg actttgactc cctcatgcgt tggttcgaat ccagctaccc cagtatac
//
LOCUS       DAZKNM010000079        11498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000079 DAZKNM010000000
VERSION     DAZKNM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.080"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..596
                     /locus_tag="ACG8M9_001683"
                     /pseudo
     CDS             76..596
                     /locus_tag="ACG8M9_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(594..1313)
                     /locus_tag="ACG8M9_001684"
                     /pseudo
     CDS             complement(594..1313)
                     /locus_tag="ACG8M9_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1264..1473)
                     /locus_tag="ACG8M9_001685"
     CDS             complement(1264..1473)
                     /locus_tag="ACG8M9_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378455.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(1455..1523)
                     /locus_tag="ACG8M9_001686"
                     /pseudo
     CDS             complement(1455..1523)
                     /locus_tag="ACG8M9_001686"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            1750..3023
                     /locus_tag="ACG8M9_001687"
                     /pseudo
     CDS             1750..3023
                     /locus_tag="ACG8M9_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3130..4161)
                     /locus_tag="ACG8M9_001688"
     CDS             complement(3130..4161)
                     /locus_tag="ACG8M9_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378456.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4168..4998)
                     /locus_tag="ACG8M9_001689"
     CDS             complement(4168..4998)
                     /locus_tag="ACG8M9_001689"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378457.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4995..5810)
                     /locus_tag="ACG8M9_001690"
     CDS             complement(4995..5810)
                     /locus_tag="ACG8M9_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378458.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5807..6631)
                     /locus_tag="ACG8M9_001691"
     CDS             complement(5807..6631)
                     /locus_tag="ACG8M9_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0378459.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6621..7721)
                     /locus_tag="ACG8M9_001692"
     CDS             complement(6621..7721)
                     /locus_tag="ACG8M9_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0378460.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7899..8684
                     /locus_tag="ACG8M9_001693"
     CDS             7899..8684
                     /locus_tag="ACG8M9_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0378461.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8717..9100
                     /locus_tag="ACG8M9_001694"
     CDS             8717..9100
                     /locus_tag="ACG8M9_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0378462.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9176..9307
                     /locus_tag="ACG8M9_001695"
     CDS             9176..9307
                     /locus_tag="ACG8M9_001695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0378463.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9276..9512
                     /locus_tag="ACG8M9_001696"
     CDS             9276..9512
                     /locus_tag="ACG8M9_001696"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0378464.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9524..9610
                     /locus_tag="ACG8M9_001697"
                     /pseudo
     CDS             9524..9610
                     /locus_tag="ACG8M9_001697"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9590..10561)
                     /locus_tag="ACG8M9_001698"
     CDS             complement(9590..10561)
                     /locus_tag="ACG8M9_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0378465.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            11213..>11498
                     /locus_tag="ACG8M9_001699"
     CDS             11213..>11498
                     /locus_tag="ACG8M9_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0378466.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3430 a         2643 c         2194 g         3231 t
ORIGIN      
        1 tttcaactca aaaagagact cacttttggt aaaatttagt cgaccaaaac atcaccctac
       61 caaaggagtc gctctatgga taatcatact agaaaattac tcggattaac agataaacat
      121 ttattttttg atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt
      181 attaaaggga gattggatga ctcaccttcc cattgtgaac actgtggatc gattcgtatt
      241 atccgcaacg gttcctatac gacacgtact cagatgttga aagtcaaaga aaagttgact
      301 attttagaat taaaacgtac ccgttttcta tgctatgatt gtgggcagac cttttctgcg
      361 aaaacggact tagttgatga acatcatcaa ctaacaaagg aactgaagca ggcgattctc
      421 atagaactgt acgaaaatca atcacgtaat ctgaaaatca gaagaagtac cgtcggctca
      481 agcgatattg gaaactctta ctcaaagatt ccacgacatt ggagccgctc aaacggcatt
      541 atcatcgact gtttaaacga ccaattagtc aaacagaaat cgtcgatgaa ccgttgtgca
      601 caatggtgtc ctgaacgtac agcgacacct tccatatcca gagcagtcgc cacatcacgg
      661 ggatggatgc catccaaatt aaaagcaacc acgccagtat gatgttcagg atcttgcggt
      721 ccgtaaatgg tgataccgcc gatcgcttgt aatttaggta aaacgtaggc aaccagttct
      781 tgctcataac gatgaatatt ctccatacca atctcattca agtaatcgat tgctcctgca
      841 atgttcggag taccagcttc aaatttccat ggcagctctt tccatgaact atcgtaaaga
      901 ttgacaaaat ctatcatttc accgccgaat tcgactggct ccatttgttc taacaattga
      961 cgtttgccat ataatacacc gattcctgtc ggcccgcaca ttttatgccc gctaaaggcg
     1021 aaaaaatctg catccaacgc ttgtacatca actgtcatat gcggtgctgc ttgggcaccg
     1081 actggactat cctcaactac aacagcctgt tcaaccctca gccgtttcaa gcacaacaaa
     1141 taaccttctg gatctggttt gccgacctga agatcatcga tcgtcacaaa ggaatcaatt
     1201 ttttccttaa tcatcggatg tgcttcaaaa aaatagtcta gctcccgcag ttcagaattg
     1261 agactactcc gattttccag tgctgctgat ccgctgctca aaatgttcag catccactgg
     1321 tctcccaacc gcttgactta tcccttcata aaaggacagt tctgctgccg tcccaacacc
     1381 ttgttgatag tctgcaaaag gaattttata gccgaactgc tccagtaaat aggcttcagt
     1441 aaatagtgat gaatactctc gtaaaacatc cattcactgt caatcatcgt cccatcaaaa
     1501 tcaaaaataa gtgctttggt catcttcttt cccccttacc caaaacaagc aaaagctgtg
     1561 tcctgctctc tctacagtat cagcataaaa actgccgtct gaaaagtcaa aggtgaaaga
     1621 aatgtggcgc tataatttct aggactgata gtaaatcatt ctatcagtcc ttttgagcat
     1681 aaaaaaacaa aacatacaga agctccgtta gaattgaatc accacaacac aatcaaagga
     1741 gcgttctgta tgcctgatct tcatcttatc aaactattac taggaatcaa agatggaaac
     1801 attcaaatca ctcatgtcga gaagaaaaaa atacattcaa tcaacagtat cgttgtttct
     1861 gccacgctca ttaaaacgat tcagccctgt cctcagtgta aacaggtgaa tcaacatggg
     1921 ctgatcgtta agaacggaaa gaaaaaatcg ctggtccttc tcaataagtg tgcgaatcaa
     1981 cggacttatc ttgctttggc aaagcaacgg tacctctgtc gtggctgtca cacctatttt
     2041 acggcgaata cctacattgt tgaaaaaaac tgttttatcg ccaaacaagt acattataaa
     2101 atattggagg aattgactga aaaacaagca atgacgacga tttctaaaca ttgcggcgtt
     2161 tcttggtcaa ccgtttcgcg aacactcgct tctctgctcc ccatgacaaa cgtgaaaata
     2221 aattggttgc ctcgctgttc attggtagat gaatttcgtt cattgaaaaa tcaagtcggt
     2281 ccctattctt tttcttgtat ggatggcgat actgggaaat tactggatat tcttccttca
     2341 cgtaagaaga aagatctcat ttcttatttt atgcaatttg aacgacgtgc acgtttaaac
     2401 gtaaaaatac tcgtaacaga tatgaacgcc tcttatgctt tgctgataaa agagtgcttt
     2461 cctcatgcca aactcgttgt ggatcgcttt cacatcgtca agcacctcat tcgcagtttt
     2521 aaggatattt gactatgagt catgaaaagc ttcgaccgaa acgatccaat acaagccaaa
     2581 cactatcgac aagtgaaggc tttatctcga cttctgatca aacgacagga cacgcttgtt
     2641 tatgataaat ggacaaaatg gcgtaatttt ggttgggcct atttaaccga aagtgaagtc
     2701 gtagaacgtc tactttctac ttctgatgaa ctacgaatag cttatgctta ctatcaagaa
     2761 attcttcaag ctttttatga caaagaagcg gacatcttct ttcagcttgt aaaaacaatg
     2821 cccaagtcgg ttccaagaga actgcatcat ataaaaaaag cgtttataaa ctatgaatcc
     2881 ggcattcgtt tagccttaga gttgccttac tcaaatggca aaatagaaaa tctgcatacc
     2941 cacatcaaag cattgaaacg aatagcttac gggttaggat gagaaccaga attttcttaa
     3001 tcaatcatct gatcacttat taaacataaa aaaacatcta aagcagaaaa tcaattctac
     3061 cttagatgtt cttatatcac tctatcagtc ctatttgaca aagagccaga aatgtttgcc
     3121 gactatacac tagctttgtt gtgcaaaatg ctgagtatca atttccacag ggattgctag
     3181 aatatctttc agctgaatgt ataaggcatc gctaattttc tcttcccgat cgttcaaccg
     3241 atccactaaa atttctttgc cctccagcgt ttgaaaaaca taccgttgaa tgttgcctaa
     3301 aataaatgat tccttccata gaaccggaat cttaaccccc tgaggaaccg gttcaaattg
     3361 aatcagctcc ggtcttaaat aataaatgca gcgattgccc ttagcggaaa actcgtgggt
     3421 atactgattc agctcatacc attcgaaacg attatagttg ccgataaatt ccgcaacaaa
     3481 tcggctctga ggattctgat acaattcctt cgaggtggaa acctgagcca cccgcccagc
     3541 ttccatcaca aaaatccgat cactgattcg catcgcctct tcttggtcat gagtcacaaa
     3601 aatcatcgtc atccctagcg attgctggat agcgcgcaaa tcccgctgca gctgttttct
     3661 aattcgggca tccaaagcac ttaatggctc atccagcagc aggactttcg gttcagtgac
     3721 caatgagcga gccagtgcca cccgttgtcg ttgaccgcca gaaagaaact ggacattttt
     3781 atctgcctgc tccgtcatat ccaccaatga tagcatctca aaaacctttt cctgaatcac
     3841 ctcaggactc agcttcttta ccttcaaacc aaaggctaca ttttcaaaaa cagtcatagt
     3901 tggaaacaat gcatactgtt gaaaaacaaa gccgatattg cgatccttcg taggcttatc
     3961 ggcaatattc tcattgtcca gcacaatctc accgctcttt ggttgttcca atcccgtgat
     4021 acagcgcagc aacgtagact ttccgcaccc gctgggacct agtaaagtaa ccagctcacc
     4081 cttttcaatc tgaatttcat caatagctaa aatctgtttt tcatcaaaaa acagattgag
     4141 atcatgtatt tctaaaaaca ttttccgtca caactccttt aagggcttct ttttcgactg
     4201 cttctcttcc ctcgtctgct taagggacag ctgttgcttt tgtcgataag tcagcataaa
     4261 ggtagttaaa gcaattccca ccataaacag caaatacaaa accatcacag cgctagccaa
     4321 atggccgttt tcgttcatcc gccgcagcat atagatgcgt accgtctgaa atcgaccgcc
     4381 aatcaacagg ttcgtcagca tgtactcccc aaaaaccgaa gaaaaaatca tcagagaaac
     4441 tagggtcaca ccgatacgga tgtttggaaa cagcacctgg ataataatcg tagacatcgg
     4501 ggcgcccaag gtttctgctg catccactaa ttccttcaga ttaattaagc gcatctggtt
     4561 gttaagacta agatagacaa tcggcaacgc cgtaataaac aatgcaccta ccaacactac
     4621 aaacattggg ataccagttt cagcatacgt tcgaagcaaa gccgttacca aaatcactcc
     4681 tggtaaagca tacggcagca ggagcagctt ttccagccaa cgattcaaac gagggaaata
     4741 caaatgaatc caaataatgg tgggaatcat caacaacaga atcgtcacca agaccacacc
     4801 tgctaataaa agtgagcgac ccaccgcagc aataaattcc ctgtccgtca gcagctgttg
     4861 gaaccattgc aaggtaaaat cactaggtaa aagcgatttt gaccatttag tggagctggc
     4921 atacaaaatc gttccaataa tcggcagcaa gtaatacagg acaaagcaga acaaaatcgg
     4981 ttttattaac tttttcacgt ctgcaccacc ttgcctttct tttgaataat caacattgcc
     5041 gttaccagca ggacacctaa agcgactgcc aacgcactgc ctaaattagg cttggcgaac
     5101 acatctcccg acactaaagc cgcaattcga atcgttaaaa gatttacatt actgctggtc
     5161 agggcatacg ctgtttcata ggtccccatg ccattagcaa ataagatgac aaaggtgctg
     5221 gcaatcgatg gtcgcaaaaa aggcaaagcc accttgaacc aagcatacgc tgatgaagcg
     5281 cccagcaatt gtgcggactc cagccattcc tttttcactt ccttcatgct gggatacaaa
     5341 aacaagaccc ctaacggaat ttggaaatag gtatacgtga tggtcaatcc ccaccaagaa
     5401 tacaaattga agtccgctag aaatggcaaa atcattttca gcacacctac attccccaac
     5461 aaaataataa aagagaatgc cagcggaatc ccagcaaaat tagctgccaa attggaaaca
     5521 aaggtgattt tctcctgcaa tttagtcgat agattcatta agcataaaca taaaataaac
     5581 gtcccgatta acccaatcac actggagctg acagcaatca gtacgctgtt aacaaaggcc
     5641 tgtgtgtaat atttgttgct gaaaatatca ctgtaattgg ccaatgtcca ctggttagtt
     5701 aaatcactgt gcaagctgca cagaaccata aaaagcagcg gcagcagtaa aaatgcacag
     5761 aagataaagg aggcaggaac cagaaccaag cgttttttca tctctttcat cccctcatca
     5821 attcttgaat gtcctgcatt ttctctgacg gtgcaattcc taataaccag caaaacaatg
     5881 gagccgccag cagctgtggt acagtttgtt ccttcagctt ttggcgttta tttgaaatca
     5941 aataaaaagg aacttcacga tgtgcagcca agctgccgcc atgcagtccg tgcgcatcca
     6001 tcccgtgatc cgcagtcacc acgacttgat aacctttttg cagccattca ggaaggtaaa
     6061 tagccaataa tctgtccgcc tgattaaccg ctgctgcata ttcatgagaa tcggcggtaa
     6121 atttgtgacc gacatcatca atattcatcg aatgtacgac caaaaagtct ggctgatact
     6181 gattcactaa ataatgggca tccgcaaata aatgcgaatc agggtaagtg tcctcactat
     6241 aaaagatgcc atgctgaatc agtcgctcct cctgatgctg gatgcgatca accaaacgat
     6301 tatagggggc ttgattgtac aactcgctgt accagtggta agcggcggct cctgtttttc
     6361 cgccagcttc cttaactaat tcaaacagcg accactcttt ggatcgctga gcatggtaat
     6421 tactatagat gccattttca taggctggaa cgccggtcat cagtacttcg tataacggac
     6481 gtgaattgct gggcagctca ctttttacct tcaataagct tgcttgctgg tgctcaacta
     6541 aatgattcat gaacccaagc tgggcgacgg ctgtgtcgta gcgacagccg tctagtataa
     6601 ctacaatcag tttgtcaagt ctactttgca taggtcagta cctccgcctg ccaaagctct
     6661 ggaagctcta cagaggtctt gttccaagct tcattgtctt caactggcac cgccgcttca
     6721 taagcatcct ccggcagtaa tttttcagct acttctttag gcagctctac cttgcgaata
     6781 ggtcgcgcat agccctttgc caaattgatt tgcccttcat cagataaaat atattcccgt
     6841 gctagctttg ccgcattggg atgaggggca tttttattga ttaaggttgt atacccgcta
     6901 atgatggagc cgtctgttgg cacagttacc tcaaaacgct cggagtcaat ttggtcacga
     6961 taatttagtc cattgaagtc ccaaacaact gccacatcga tttcaccttt ttcaagattt
     7021 gctacgctgg catcaacggt ggacaaacgg cctttttctg ccattttttt gtagtaattc
     7081 agacctggct gaatatcctt ttcgtcacct ccgttggcaa tcgccgcagc caatacagca
     7141 aattgtgctt ggttggcaat cgtcacatcc cccacgctaa cttgatagtc accctcttgc
     7201 agctccttcc agctagtcgg tgcttgcttc acattctctt tatcggtgat aaaggaaatg
     7261 gttcccgtat agcttaacaa ccaatcccca tcatcatcct ttgcccaatc cggaatttcc
     7321 tcccaatagt tggtcttata aggtaaggtt aactctttgc tttcagccaa cggaccgaag
     7381 ctgataccga catcaccaat atcggcggtt ccattttttc cttctgattc aaacttagcc
     7441 aattcttctg cactggacat atccgtatcc acgtgactta agccgtattc tttttcaagg
     7501 tcgttccaag tgcctaccca atttgcccaa gtatccggca ttcccaagct ggcaatctct
     7561 ccttcttcct tggctttttc tataatcgta tctaaagagt cgtctgccgc agccgattgc
     7621 ttcgttgctt cacctgagcc gcagcctgtc agcaggatac ttactgttgc tgcacaaaat
     7681 aaaccgatag tcatgttacg aattttttca ttcattttca ttcattcttc tcccctttcg
     7741 ataattccat tctaacaaag ctttgtaatg agagaatcag tttcttgaaa aggtttcatg
     7801 aacaatgtaa aggttgtata tggtttgtaa atttcgacga attcccgtta accttctaaa
     7861 gcttttgcgg tatgatagta gaaaaaagga ggctccctat gcagtattat gatcagcatc
     7921 tccacacgta tttttccttt gattcagagg aaaaatttga aaactattta gcctatcagc
     7981 cggagttttt cgtttctacg gatcactttg atttaaacaa tccatctacc ggaaaagatg
     8041 acattccgga ttatccagcc tatataaaca aactggactc tttaaaagaa acctattcca
     8101 ccaaatttct gcgaggtatc gagattggcg ttgttccagg acaagaagag cggatcgtgg
     8161 attacctcgc tcagcatcct tatgatttaa agctggtaag tattcatcaa aatggccgct
     8221 ttgattatat ggacgatgtg gttttaacca aagatcccat tcaaactacc aaggaatatt
     8281 ttgatcagat gttgcaggtc ttgacggatt ttccagaagg agatattttg actcactttg
     8341 attatggatt acgccgcttc accttcacag cggaagaatt cgcggagcat tttgaggctc
     8401 agctgctaac gattttcaaa aaggttgttg aacgagaatt ggcgatggag ttaaatgcca
     8461 agagcttctt gaaatacggc aacgaagacc tttaccgtta tgccgttcct ttgtatcaaa
     8521 gtgtcggcgg aaagctcttt accttaggct cggatgctca tgttgccgaa gattatcagc
     8581 tgggctttaa aaaaatgagt cagctgttgg cagactgtca ggtagaaaag ttgctagtca
     8641 ttcaaggaaa agaacgccgc ctgacgccgc tgccgatttt gtgattccta tataatagaa
     8701 gaaagtggtg aaaaaaatga gcaacctgtg ggactacaat caagaggcac ccatccatta
     8761 tttaattgcg cgacattggg atgcgttgaa aattgaggcg gtttgccgat ccttgttagc
     8821 agccgttcca aaacaacaac tggaaaattt tttggtggct gacagcttgc agcgggaaaa
     8881 agttcaggct tattttgcag cctttaaaga tcagcctttg gaatatttgc atgcgcagtt
     8941 tcatcttttt tatcaagtag ccgccccaga tgactacaac gatttacgag gacagctgca
     9001 attgaccttt caagcggatg aaaccgccta taccgtttta ttgggaatgg ctcgccttgg
     9061 tgatcaggcg aaggtcgagt ggcggatttt cgatatttga tggcaggatc aaggcgtttc
     9121 agttgcaaac cttcctctct ttcaattaga aaaaccgcag caggaagaag tttgtgtgct
     9181 ggtcaccgat gctggtgaaa ttcgtctccg cttatttcct gaccaagctc ccctggctgt
     9241 agccaactgg cgctccctag ctgagcaagg cgtttatgac aacacacctt tcggctcgtg
     9301 tcattaagga atttgttatt caaggtggcg ctttggatgg ttctggtgat gaagctgttt
     9361 ccagctatga gggctttttc gctgatgaag tgcatcgcgg actttatcat tttaatggcg
     9421 ccttggcctt aggggaccac ggcccccaca ccaacggcaa ccagtttttt attgtgcaaa
     9481 acacaaaggc gcaggctgac ctgctaatgt aaagcaagca tacttggagc ttggaggact
     9541 accggagctg gatggtcgtt atactgtttt tggacaggta taccaagggt tagaaatcgt
     9601 agcagcgatc gcagctcagc cgacaaacag tgaagatgta ccgattgagc cagtgttcat
     9661 tcgacaaatt aacttcgaaa aaacatcgta aaaagacact cactgacttc agcaaaagct
     9721 ctattcttga gctggctgca gctagtgagt gtctttcatt tatccataag ataaagcttg
     9781 gattcaataa ataaataaat gttttggtta aagctgtgac cagcaaatga ggaaccgtgc
     9841 gagccagttg atctgccaat gcaaacattt ctttaggtga aggcgctggc gtgtctgcca
     9901 acggatactc cagtcccaat gcttggtaac cgtgcatacc atagcagtgg tacggtagta
     9961 attctaacgg tacgaaccga tcaagatctg ccacaaaaca agcaatcgtc agcagctcgg
    10021 tcaggttcat attgatacct ggaaccacag gaactcgaat tcgcacttcg cagttaggat
    10081 actcaattaa aaagcgacga atattcttca aaatttgttg gttatctaag ccggtccatt
    10141 gtttgtggag cttgctggta aacattttta catccacaaa caatgtatcc acatagggag
    10201 ctaccttttg gatttcatta taagcaccac aaaagctggt ttccagtacg gtattgattc
    10261 caatgtattt cgatttttgc aaaatttctt tagcaaaatc cgcctgaacc agtgcctcgc
    10321 ctccgctaat ggtcactccg ccatccgagt gaaagtaaaa aacagcatcc ttctcaatct
    10381 catccattac ctcttcagcg gtcatggtct ggccaaatcc tttttcaatc tgcttgcttt
    10441 gtgattcggg aatcgaacac caggcacagc gtaatgggca gccctttaaa taaacaacgg
    10501 tccgtaaacc agcaccatca tgtagagaac cctgttctat tcttaatact gttccttcca
    10561 tatagatttt tgaattacac actagtttaa actaaaacac acaaggaaaa acttaccatt
    10621 taatcataaa attagctcat ataaatatac taggatgtaa aatagactgg tcaaaatctg
    10681 gtcaaaaaag aggactaata caggtagtcc tcttttttgt tattttaata ttctaaatca
    10741 ttttttattg gtttattccc ttattcgatg tagactcaaa tcactatatc actagatatc
    10801 aaacggcgtc atacggaatc aaaaacatca aatttgacct gttaaatggt cattgaataa
    10861 cattcaaaat caataaaacg gagtcagaca cggagtcaaa aacgctgaga gtaaaaaata
    10921 tatacagcct tgtaggtaaa gtaccacagg gctgtatatt ttttatttca gattacctta
    10981 aatgattcca tttacttttt atctcatttt ttcctactag ttagtatcaa atactctcct
    11041 attttctcaa taaactattt ttggatacat ggtatctgaa acagctaaaa ccttgtcatt
    11101 acagcaaaaa atcacgttat ccagactgta gacaaagtcc attttatgga tgatgtctac
    11161 agtcttttct tttataatag atttatgaaa acgaatgcaa ggagtgatag ctatgatgtc
    11221 taaaaacaat acgaatggta gaaatcaatt tgcgatgctt acgattgatg atctagttcc
    11281 tcaagatcat ctcgttcgaa agattgatgc cgcgcttgat tttgaattta tttatccaat
    11341 cgtcgaagcc acttactccg atttaggtcg cccaagtatc gatccagtga ttctgatcaa
    11401 attggtgttt attcagtatt tattcggtat ccgttccatg cgacaaacca ttaaggaagt
    11461 cgataccaat gtcgcttacc gctggttttt aggctact
//
LOCUS       DAZKNM010000080        11337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000080 DAZKNM010000000
VERSION     DAZKNM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.081"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACG8M9_001700"
     CDS             complement(364..1482)
                     /locus_tag="ACG8M9_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378467.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACG8M9_001701"
     CDS             1715..1837
                     /locus_tag="ACG8M9_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378468.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACG8M9_001702"
     CDS             1953..3260
                     /locus_tag="ACG8M9_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0378469.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACG8M9_001703"
     CDS             3379..4578
                     /locus_tag="ACG8M9_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0378470.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACG8M9_001704"
     CDS             complement(4605..5873)
                     /locus_tag="ACG8M9_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0378471.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACG8M9_001705"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACG8M9_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACG8M9_001706"
     CDS             complement(7090..7593)
                     /locus_tag="ACG8M9_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0378472.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACG8M9_001707"
     CDS             complement(7716..8480)
                     /locus_tag="ACG8M9_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0378473.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACG8M9_001708"
     CDS             8588..8863
                     /locus_tag="ACG8M9_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0378474.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8M9_001709"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8M9_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0378475.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10609)
                     /locus_tag="ACG8M9_001710"
                     /pseudo
     CDS             complement(10430..10609)
                     /locus_tag="ACG8M9_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3401 a         2387 c         2002 g         3547 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaata
//
LOCUS       DAZKNM010000081        11208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000081 DAZKNM010000000
VERSION     DAZKNM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.082"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1475
                     /locus_tag="ACG8M9_001711"
     CDS             51..1475
                     /locus_tag="ACG8M9_001711"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0378476.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1774..2481
                     /locus_tag="ACG8M9_001712"
     CDS             1774..2481
                     /locus_tag="ACG8M9_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0378477.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2468..2791
                     /locus_tag="ACG8M9_001713"
     CDS             2468..2791
                     /locus_tag="ACG8M9_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0378478.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2880..3917)
                     /locus_tag="ACG8M9_001714"
     CDS             complement(2880..3917)
                     /locus_tag="ACG8M9_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0378479.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4238..5221)
                     /locus_tag="ACG8M9_001715"
     CDS             complement(4238..5221)
                     /locus_tag="ACG8M9_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0378480.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5636..6406)
                     /locus_tag="ACG8M9_001716"
     CDS             complement(5636..6406)
                     /locus_tag="ACG8M9_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378481.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6399..7334)
                     /locus_tag="ACG8M9_001717"
     CDS             complement(6399..7334)
                     /locus_tag="ACG8M9_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378482.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7650..9299)
                     /locus_tag="ACG8M9_001718"
     CDS             complement(7650..9299)
                     /locus_tag="ACG8M9_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0378483.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9416..>11208)
                     /locus_tag="ACG8M9_001719"
     CDS             complement(9416..>11208)
                     /locus_tag="ACG8M9_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0378484.1"
                     /translation="RQPVYSRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERN
                     GIHISKGLLRRLWKKILQNQAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKR
                     MADALKLKSNEVLVFNTTTENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKN
                     AYRMIATGFEYFDEPAYYELDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISN
                     NKYKLSFKDGQLNLIVGDRQYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVY
                     KDSLQETLVVYGKAQLPKDLKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVY
                     SHRMRLAINVLDKSEKSIAQIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVA
                     ENDHYFTFYADGLKEYERIENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMM
                     ETPLAQEIGEYKVTFGFNVEEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNK
                     IWPLQNELDVPRKFSLIELPEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILS
                     LGTVVNALENKQELTTIPPYDYLSILINER"
BASE COUNT         3290 a         2295 c         1992 g         3631 t
ORIGIN      
        1 taagttgtaa gcggtgttat cattaagtgg aaacaggagg taaaaaaatt atgacaacta
       61 tgaaaaagct aacttttcca gatgattttt ggtggggttc cgcttggtca gctgagcaag
      121 cagaaggaaa aggagaaaca ggaaaagcag agactatctg ggatcgctgg tttaaagaac
      181 aaccttaccg gttttatcaa cgaatcgatt cctctattac aaccgatcac atccatcaat
      241 ataaagaaga tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc gtttctattt
      301 cttgggcacg aatgtttcca aatggcggta ccggaaaagt aaatcccaaa gccatttctt
      361 tctatcgaaa attattttcc gaaatgaata ataacggaat caaagtattt gccaacttat
      421 atcattttga catgcctgct actcttcaag ataaaggagg atgggaatca cgtgaagtag
      481 ttgatgctta tgttcattac gctgaaactt gttttaaaga atttggtgac ttagtttgtc
      541 attggtttac cttcaatgaa ccacttgggc ctattttagg tacttatctt gaagattttc
      601 attatccaaa cctcattgac tttaaacgcg gagcgcaagc agcattcttc actatacttg
      661 cacacgcaaa agcaatacaa gtatttaaag aactagctct tcctagtaaa ataggcgtta
      721 ttctaaattt gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg aaagcagcag
      781 aaatcgctga tttattttat acgcggagct ttttagatcc tatggttaaa gggaattttt
      841 ctaaaaagct tatcctgtta ttacaagaat ataatcagct tcctgaagat tatacgaaag
      901 aagatcttgt atgtatctac gaaaataccg ctcaaattct tggactaaac tattacgagc
      961 cacgtcgcgt caaatctcgt tcttctatgg tggatacatc tagtccattt ttaccagagt
     1021 ggtttttcga accacatatc atgcctggac gacgaatgaa cgaataccgg ggatgggaaa
     1081 tttacgaaaa aggaatttat gatttgtgca tggatatcaa gaaccattat ggaaacatcg
     1141 aagcatttat ctcggagaac ggaatgggcg ttgcggatga agaacgtttc atggatgata
     1201 cagggcaaat aattgatgat tatcggattg agtatatcaa agatcatctt gcttacttat
     1261 ggaaggcaat aaatgaagga tgtaacatta aggggtatca tctttggaca tttattgatt
     1321 gctggtcatg gatcaacgcc tataaaaacc gatatgggct ggtttcactc gaccttacta
     1381 ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa gcaattaagt gatacaaatg
     1441 gatttagata cgatgtagac ttgttagttg aataatattc taaaattcca cttgaagtga
     1501 acacattaga aaacaagaat aatgagaaat accaataaca aatacttttt cttcatactt
     1561 catttatact tgtcagtata agagactgtg acaaatgtag tgatcctcag aagttaggtc
     1621 tggaaatcta acttgagaag atttttttaa actacgtcca gtctttttta tttgtttaaa
     1681 attgaagttg acaagtcttc attaaaaatt tagaatattc acaagtttga cgacaattat
     1741 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
     1801 gcgatcaaag acacgttacc taccgttttt ggttatatcg gtattggact tgcatttggt
     1861 atcgttggga aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc cctgctgatc
     1921 tatgctggtt ctgcccaatt tatcacggtc agcatgcttg ctagccatag tccactgctc
     1981 tccatcgttc tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt
     2041 gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca
     2101 gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt
     2161 cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt
     2221 ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc
     2281 gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa
     2341 cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt
     2401 atcccaagta acttagtgat tttagttgta acactgattg ggtgcggatt gggggtgtgg
     2461 atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac
     2521 ttggctttct aggatcctac catttgtctt actgaaaaaa ttcgatttgc cgcaaccgtt
     2581 gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct
     2641 atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt
     2701 tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggtaggaat
     2761 tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta
     2821 ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct
     2881 tatccgttga acggaccact aacaacgact tttcctaata tatgtcctgt ttctacgtct
     2941 ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta
     3001 ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt
     3061 tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat
     3121 tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt
     3181 ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc gggtaatatc gaataataca
     3241 gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga
     3301 taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca
     3361 gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc
     3421 ccagctccgt tgatcactaa gatttttttc ccgcggttgg cattttcttc tggagtcaga
     3481 ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcggct
     3541 tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac cagttggtat
     3601 tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc gccgacagaa
     3661 aatttttgca cttcatcacc tacagcaaca actttcccga ctccatcaaa ccctaagata
     3721 cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat
     3781 actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca
     3841 tcaataaagc tttttggatc atcgatcgac aacccttcat aaaaaccgac taccttcatt
     3901 aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag
     3961 ttttcatttc aatatatata gtatgctatt tgttccactt ttattcatca taattattct
     4021 cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg acaagacttt
     4081 tgtcacaacc tcttttaaac ggtgttcaat caactgaccc tcggtattcg ctcaaaaaac
     4141 gacaaaatat gaggagctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt
     4201 tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg aaaagcgttg
     4261 gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc atcagtagat ttcgtattca
     4321 tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt ttaaagaaac
     4381 gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc agatctagtt
     4441 gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcggttct ttttcaaatg
     4501 cataaggatt cttgaaaatc ccccggccaa tcatgatgcc atctacgcca tatttttcgg
     4561 ccaattcttc tcctgtctgg cgatctggga tatctccgtt gatcgtgatc agtgtttgcg
     4621 gagcgatttg gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt gcatctactt
     4681 tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct tgatgaagta
     4741 ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc gtcttgacgc
     4801 tgacaggcaa accgccagct ttagccgctt caatcaattg tgccgctact tctgggcgaa
     4861 ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag cccatgttga
     4921 tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta aaaaattctg
     4981 gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag gtcaatcttc
     5041 ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta aactcagtaa
     5101 agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca tctgttacat
     5161 cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca
     5221 tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc aaaaacattg
     5281 tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat gtttctgatt
     5341 gagcaaaaaa agactgtgac aaaaatctta tcacaattcc ttttccggaa taaatggtgt
     5401 tcaaaagctt gcatctgcgg taataagtcc aaccaaacga aaaataggag gagctatttt
     5461 tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct ccactaaacg
     5521 gtgttcaatc agctgaccct cggtattagc tcaacaaacg acaaaatatg aggaactatt
     5581 ttcccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct cttttctaac
     5641 ttctcaatcc tcttccttta ttgatcaaat accaagtaac ggcgtacaaa acaacgatga
     5701 atagcacaag tacgatcatc gatgttgcta ctggcacatc gattgttcca aggaatccgt
     5761 aacggaaacc ggagatcata taaacgatcg gattgacttt cgaaacagct tgccaaaatg
     5821 gcggcaacat agagatggca tagaaaacgc cacctagata agttaacggc tgcaagacaa
     5881 atgtaggcac gatcgataca tcgtcataag actgagcaaa aatcccgttg agcagtcctg
     5941 ctaacgaaaa caagattgcc gtcatcaata atgtaatgat gaccatgaac catgaataca
     6001 catgaagtgg tacaaagaac aaggaaatga tcgttacgag agagccgacg agaatactgc
     6061 gacccactcc gccaatcaca aagccccaaa tgatcacatg cgtaggaaca ggcgctacca
     6121 agatttcttc gatgtttttc tggaattttt gagagaagaa ggacgaagaa acgttggcat
     6181 acgaactagt gatagcagac atcataatca aaccaggcac gatgaattcc atatatgaaa
     6241 aaccacccat gtcaccgatc cgaccaccga tcatttttcc gaaaataaca aagtacaagg
     6301 atgtcgtgat cacaggcgga accagtgttt ggacccagat acgcaaatag cggttcgttt
     6361 ccttgatagc taaacttttt aatgcagtaa aataaagatt aaacattttt ctcctccgta
     6421 tgattatttt cttctgtgat tttcaaaaac agttcttcta aacgattcga cttattcctc
     6481 atagaaagaa ctttgattcc ttgtcttgtc agctgatcga agattccgtt caccccttga
     6541 tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa atgtacaagc gtatccttca
     6601 atcacaggtt tggtctcata tggcgctaaa tcaaagataa atgtttcaaa ttgcagtttg
     6661 gatagaagct ctttcatact agtattctcg atcagttcac ctgattggat gatcccgatg
     6721 ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg tcaaaatgat cgttgtacca
     6781 ttttcgttca attctcggag aaactcccac atctcgcgtc tcaattcaat atcgactcct
     6841 gctgttggtt catccaagat cagcaaacgt ggttcatgca tcaatgccct agcaatcatc
     6901 agtcttcgct tcattccccc ggaaagcata cgtgcacgga catttctctt ttcccaaaga
     6961 ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt ctttgcgaga gacaccgtag
     7021 tagcctgctt gattcacgac gatttgctgg accgtttcaa agggattgaa attgaactct
     7081 tgcgggacta atccaatctg ttgctttgct cggactagat ccgtatccag atcataaccg
     7141 aagacattca cttttccaga tgttttattg actaaagaag taatgattcc gatcgttgta
     7201 gacttcccag ctccatttgg tcctaatagc gcatagaaat ctccttcttt cacggaaaga
     7261 tcgattcctc gcaatgcttc gaccccactt ccataaacct ttttcaaatt atttatttca
     7321 agcgcatttg ccatttattt cctatctcct tatagacatg attctttatt gtatcatgga
     7381 atacagcaga tgaaaaaaca acttggttcc agcgaaaaat caggttgctg ggctcaggaa
     7441 aatctcctaa tgaaagacat caatcggacg aagtcagaca accttttcag aaaaaacaaa
     7501 tcgaagataa aggattacta ttcatatgtg caacaaaagg agcgtgagac aaaagtaaag
     7561 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcaaaagc caagattttt
     7621 gatccaataa atactttcga tcacttcgcc tatcccaata catgccactg ccgcttttca
     7681 tcaaataacg cggcttccgt ttttgcggtt tctactaaag tctctgggta atccaaaact
     7741 ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt cttttaattt gaaatcaaaa
     7801 ctgattcctt ttccttctgt tacttgttct tcaaagtgga cattctcaca ataattcttt
     7861 aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg atactccgca
     7921 agccaccgaa cgactgaagc ggaagaagca atccgttcga tcgtatttgt cccttttaaa
     7981 atctcatcta cgaagcagta gcagcgttct ttggaagcca cttggtccaa caaacgtttg
     8041 atcgatttga tttctgaaac aaaataactg tcgccttcaa ataaatcatc ttcgattgcc
     8101 atggaagtca acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc tgtttggatc
     8161 gtctgtgcta gaatacagct gatggctata cttttgacat acgtagattt tcctgatgca
     8221 ttggaacccg tgactaatgt attcttctgc cagttgaccg gattcactac cgcttctttt
     8281 aacaatggat gatagatatc tttcgctgtc acaccgccct cagtaaactc tggtcggcaa
     8341 gtgatgggca tataagtacg gtaattcaat attgcagcgg ctacttctaa ttctcccagc
     8401 aattcccaca gcttgatggc ttcagttgaa tgcttttcta atttagtgag aacgaaatga
     8461 taggagataa atggcagcat gaacatgatg ttgacatatt caaaaatcaa ctctgcttca
     8521 ctcccactct tcatacgaaa tgaaaaacct aatttaggga tgtctcttaa tggtttagtt
     8581 tgctgtttca gctcttcttg aagtggtgtc ggcagttttg ctagctgatt ggcggctgaa
     8641 atcgttgaaa ccaaataact catgctattc agctctgttt ctagtgtttg tttcttcact
     8701 agataataga ccgtattgaa tactaaccca ccgattgcca aatagattcc tagtacttgt
     8761 cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc caagaaaaat aaataaacct
     8821 acgcgtccaa tctttttact ttttccatcc gctagatatt gttttgcaaa attatgatct
     8881 ttttttccta aacgagcgaa ttgatattgg attttttctc tcagttgagg attttcctga
     8941 taaaaagcaa tcagtttttc taactgctga tcttcaccaa aatcaaagct gcgtaaacgt
     9001 tgatacaacg cttctgaacc tacactagaa taggtactgt tgattccttc gaatacttcg
     9061 aacatatcca ggtcatacca agtaagatca tcgatttcgc tatcccatga acggaacttc
     9121 ttctccgtga gccatgcttc cttcaagctt tcttctttat caaatctcgg ttgataagga
     9181 attttttccc atttgctacg aactgtttcc tttaatttca aacgtgaaaa aatgtctaaa
     9241 gcaactaata aaataacagc tgcaataata attaaaacga taaattctct ttgacccata
     9301 tccctctccg ttcctttatt tgttagcttt agtatagcac tgttgcgcta tttgaaaaga
     9361 gggtaaaaaa acacctttca aaataaaagg tgtttttttc ttgtatatta catatttatc
     9421 tctcatttat caaaattgac aaataatcat agggaggaat tgttgttaat tcttgtttat
     9481 tctccagtgc attgacaact gtcccaaggg aaaggatagc ctcaggtact attttttctt
     9541 gttctgttgg attagctaag cgaagaacaa atccttgttc atgaaaataa gaaggataaa
     9601 tcgatgatac aattaagtct tcaggtaatt caatcaatga aaatttacga ggaacatcaa
     9661 gttcattttg taatggccaa atcttgttat ctaacctgtg aataaataca ttcagtttct
     9721 gcttttgata agaaatagat tgttctaacc gtttttctgc ctgttttgcc actttgaact
     9781 cattaaatct tccttcttct acattaaatc caaaagtgac tttatactca cctatctctt
     9841 gagctagcgg tgtttccatc atgacatgcc cttcatttgt cgtatctcct gaagctcgcc
     9901 caggtcgcca agcaagatta ggttttccaa gttcccctgt tgttgaaaat aaggttatga
     9961 ttaattgatt ttctatacgt tcatactctt ttaaaccatc tgcataaaac gtaaaataat
    10021 gatcattttc tgccacagag acactcttat cgaatatttc taaattaaca ggtttctcta
    10081 ccatagtttc tgaccaattc ggaggaatga catcaggggt attttcaata aatccgtttt
    10141 gaatttgtgc aattgatttt tcagatttat ctaaaacatt aattgctaag cgcatacgat
    10201 gcgagtaaac agtattatga attttgagtg tgccttcaac gatttgactt tctcccagac
    10261 tgaacgatat ttcataagat atttcttcca tttcgggttt ttcagataaa cgatctttta
    10321 aatctttagg aagttgagct tttccgtaaa caactaatgt ttcttgtaaa gaatctttat
    10381 atactttagc tgtttctaat tttagagata gttctgtatc tccttctaat ggtgaataat
    10441 catacgtatc gccatcattt gctgaatcga tcaaatgaac gaaatcaaga tattgtctat
    10501 ctccaactat caaattcaat tgcccatctt taaaagaaag tttatattta ttgttactta
    10561 tctcagtttt gtctatctcg attgctgttt ctaatttttc attatttttc tcaaattcaa
    10621 caacagtgta tcctagtgct ggcacattta catttatttt tatgtcaagt tcataataag
    10681 ctggttcatc aaaatattcg aaacctgtag caatcatccg ataagcattt ttccgttctg
    10741 gaaaataaac ttctttttca atgatattat agggactacc tttgaagtgt attttctttg
    10801 aatcagctac tacatgcacg attttcgatc cactataatt ctcagtcgtt gtgttgaaaa
    10861 caaggacttc attcgatttt agctttaatg catcagccat ccgttttaca ataaggtttt
    10921 cgattccgtc agctatctca ttagcttctt tgattcgatg gaaaatatct tccgcaacat
    10981 tatctgaaac acagccacca atactatcat gagcctggtt ttgcaaaatc tttttccata
    11041 accgacgaag taggccttta ctgatatgga taccatttct ttctgcaatc agcatgagcg
    11101 gctcaactcg ttttagaatt ttttgttcca atttaaaatt ttctattttc atttttgttc
    11161 ttactgagcc aatagaacga tgaacacgag agtaaaccgg ctgacgaa
//
LOCUS       DAZKNM010000082        11206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000082 DAZKNM010000000
VERSION     DAZKNM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.083"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            368..1807
                     /locus_tag="ACG8M9_001720"
     CDS             368..1807
                     /locus_tag="ACG8M9_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0378485.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2102..2320
                     /locus_tag="ACG8M9_001721"
                     /pseudo
     CDS             2102..2320
                     /locus_tag="ACG8M9_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2569..2784
                     /locus_tag="ACG8M9_001722"
     CDS             2569..2784
                     /locus_tag="ACG8M9_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378486.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3001..3257)
                     /locus_tag="ACG8M9_001723"
                     /pseudo
     CDS             complement(3001..3257)
                     /locus_tag="ACG8M9_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3444..4355)
                     /locus_tag="ACG8M9_001724"
     CDS             complement(3444..4355)
                     /locus_tag="ACG8M9_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0378487.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4374..5378)
                     /locus_tag="ACG8M9_001725"
     CDS             complement(4374..5378)
                     /locus_tag="ACG8M9_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0378488.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5375..7498)
                     /locus_tag="ACG8M9_001726"
     CDS             complement(5375..7498)
                     /locus_tag="ACG8M9_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0378489.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7503..9950)
                     /locus_tag="ACG8M9_001727"
     CDS             complement(7503..9950)
                     /locus_tag="ACG8M9_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0378490.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9937..10326)
                     /locus_tag="ACG8M9_001728"
     CDS             complement(9937..10326)
                     /locus_tag="ACG8M9_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0378491.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10386..10889)
                     /locus_tag="ACG8M9_001729"
     CDS             complement(10386..10889)
                     /locus_tag="ACG8M9_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0378492.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10902..11126)
                     /locus_tag="ACG8M9_001730"
     CDS             complement(10902..11126)
                     /locus_tag="ACG8M9_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378493.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
BASE COUNT         3265 a         2359 c         2017 g         3565 t
ORIGIN      
        1 gaattgaata tcatagcaat gtatttgaaa agcaaattgg aaaacaaata ggtgcaacac
       61 cataaatatg aaaaaattgg gaaaaagtag aatggaaatt tgcttacgtc tcacgagata
      121 gctcacacta ttaatctgaa gaacaatgaa gtcgactttt agaaattgct agaagaaagt
      181 aaagcgaatt atcactagtt actatagagt aagttcaaat aaagcatctc caacttacat
      241 ggttacatga ggatatcaaa tcgaaattac tcaagcttcc tatggctaga gtcgcgagaa
      301 gttgttgggc agtagacata ggttatattg ttcttagcta cacgatcatt agaaaggaaa
      361 tcctattatg tatagttatc aagaagatgc aaaagtccaa aaaaatcgct ggtggatttt
      421 gattgctata tctatgttta ccttcatgtc cacattggat ggcagtattg tcaatattgc
      481 actacctacc atctcagatg atatgggcat accaatgaat caagcggaat gggtagtgtc
      541 cgtttatctg atgattgtct gttcttgttt gttgctgttt ggtaaaattg gcgatagccg
      601 tgggaaaatc aaagtatttc ggattggtac gctgatcttt gtcttgggat cgctgttgtg
      661 tgggttcaat cattcgctag gtattttatt gttcgcacgg gtggtacaag ccattggtgc
      721 tagtatgact atggctacaa atgccggaat catcacggaa gtctttccca tacatgaacg
      781 tggtcgggca ttgggagcaa taggtgcgtt tgtttcatta ggctcgattg ctggaccagg
      841 tcttggcggc ttgattttgt cccaattttc ttgggcgtac attttttgga tcaatgtacc
      901 ggtgggaatc gtaacgatgt tgattgctga aaaattctta ccaaaagata tcaccatgag
      961 cggccataca atcgatggag ggggctttgc tgccattgcg gctacgatta tcagcttttt
     1021 tggagcaatc tttttggggc aagaaagtgg ctttttccat ccagtccccc ttgtactgtt
     1081 cgtaatttca gtgattgcct ttgtcagctt tttaaaagtt gaaaagaaag cagcctctcc
     1141 attgatttct ttcggtattt ttaagaacaa gatttttacc atgagcttga ttacagcctt
     1201 gttgattttt tcttccaact tctttgtcaa tgtagtgatt cctttttatc tgcaaaatgc
     1261 tcgcgggttg agtcctagct atgctgggct gttgatgatg gtctttcctt tgatgatggt
     1321 cgtcggggcg cctttaagcg gctacctgac cgaccgggtt ggcccggaga ttttggtatt
     1381 agtaggcttg ggcttattgt cggtaaccca ggtcatgtat atgtttttgt cagtggactc
     1441 tcctttgtgg ctatacattg tcgctactgc catcatgggt gccggcaatg ccttgttcca
     1501 gtcccccaat aatacgatgg ttatgagctc ggtcactatc gaaaacttgg gactggcagg
     1561 ctcgatgaat tcctttgccc gaaatttggg gatggtcagc gggatttcgc tttctacgac
     1621 ggtcttatat caagcgatgt ctgctaagat gggccagcaa gtaactaact atatcgcaaa
     1681 tcggccggat gtctttttat atggaatgaa gattaccttt ttaggcagct ttgttttgtg
     1741 ttttgttgcc tttttcctga ctatctggcg tatcaaaaaa caaggaggta ttcagaaaaa
     1801 tgtctgaaaa aaccactgtt gaagaatttt tggaggcctg cgtatgggtg agtgggattt
     1861 ccatggatga tggaagatga agtgaacttc taaaagccga aaaactcaag tattatcttc
     1921 aaaatactag tgctcaatga gatgcttgat ttgatcacaa gaagactgcg aaagccgctg
     1981 gcaaccgcaa gctaggtatc ccaccgatgg actacgtttt agaggctact ggttatgaat
     2041 acggagccaa cgctctcatc tgcataagaa tacatcaccc agattatgta aagattttcg
     2101 atacttctgt caaaaaatca ttaagataga taaatgattt ccgtctagtc aaattggttc
     2161 agaatgtaga cacacagatg gtaagaagtc actcacaatt cgagaatgga cttgtcctat
     2221 ttgccgtata catcataatc gaaacgtcaa cgatagtatc aatattttga ccaagggcta
     2281 tgaatacaag cattgcctta gaaaacatca agaaccttag gaacaacggg gatagcttgg
     2341 ttaataagag ataccgctgt ttaggcttgc aagtatactc ttttatcaag aattccgtgg
     2401 ctttagtcat gagaggctta atactatgaa aattttcttt agcatttttg atttaggttc
     2461 cttttctaat tataataaga tgaccactac agtaacctac tgaaatatat aaacagccac
     2521 tgtagtacca ttttcaaagt agtctatgag aggagaatct tcattcttat gaacgctgga
     2581 attgcggata tgaatttaat aaaaaaaacc cttaatgatt ttacttccaa ttcaatatca
     2641 aaaggtactg gtatcaattt aagcacaata aaaaagctga aaagtggtga aagatcagtg
     2701 gaaaagctga atcttttaga tgctataaaa ataacggaat ttgctatgaa aaatggaaaa
     2761 gctgagattg aaatatggag ataattatcg tctagtgaat tacttgccac ttagctgaat
     2821 ttaacgatcc ttggtgcttg aagggggaga ttcttgggaa tagagcatac ttgcaagcct
     2881 atgtctttac taacaacgat atctcttatt aaccaagcta tccccgtagt tctaaggctt
     2941 ttacttttat ctaagcaata atcgtattca aaggaaattt ttgaccctta gaaagtctca
     3001 ttttatcaga cgtgagctta ggcattgctg aattttgtta attcggtttt aggttgtgct
     3061 ttgagcataa tatgaaagta ttctttatca cgatttcatt catctaaggt aatgtgatgt
     3121 gattctgaaa ttctttcaaa ggcagttttt gcataagctg acattacatc atcaatgaca
     3181 tgtttacaat gtttcactac taaaataagg gggtaaccaa gaagaaaaac tgaatgatta
     3241 tttgtatcta attccatggt atatcaacca tttcctatat agactgatta tgacatggat
     3301 taaaataaaa aggcactatg tcttaattgt cggttttcga gttaccgaat ggcattcatc
     3361 acccacctat agaggatggg cgacttctgc ctgaaataag ttacactact tataacttct
     3421 aataaaatta gattgagtca ttattattta acatcaacga ttttccagtt tccatttttt
     3481 tgtaatacta atttgtactg aaagtagcta gtggtttttg atacattatc aaggtatttt
     3541 atctttgtac tcactagaaa attttttccg ttttgtagat aaactggatt ctctaactcc
     3601 gaaaacttta agtcaatatt gattggtcgc atcgcaccat tttcaacata atactccaat
     3661 tctttttcag aagctgatgg ataaagttta aaaaatgttt ctaaaaaatc ggtgatttct
     3721 tccgttgttt tggcatcaat ttctgaatcg ttttctactt gttttgcttc atattttgcc
     3781 ttgtctggct ttccaatcat tgttggcaaa ctagttacta aatgattcct gttttcatct
     3841 tcaaagattg tcactcgata tgcagaagaa attgattgcg tcttttttcc ctctgtaata
     3901 tgttgctcca ctaaaaatga aacgacaaat tcttccgagt gtttttcaac atctagaatt
     3961 tttacagatt gaacttcgga actcgtggga atatctgctc gaaccatatc ttggattaaa
     4021 gcgagaccgt tttctgtaag gtactgttcg agattactca ttctcttttc aattgcttct
     4081 ttgttatttt tccatgaaaa atattctttc gcaaaatcct ctgtaaaact ttcaacgcca
     4141 gaagtattca ctaccttctc ttttataacc actttttcat gagttgtatg ttgatcaatg
     4201 gcggtgaaat ttttataaac gccaaaagca agactacaag acaataaaag ccaaagggca
     4261 agcaccattt ttcgatgctt acccacggat acaactcgtt cttttttctt ttttgggctc
     4321 ttttcctttt gattccgttc aatttttatc ttcattcttt ttcctctttt ctcttatttg
     4381 tatcggccag ctccgataag atgctgccgc caatagtttt ccgttagatt cgtccacccc
     4441 actggatctc ctgcatgaaa catgcgattc tcccctgcgt aaatgcccac atgggtcaca
     4501 taagtaccgg catcataggt tgaatgaaag aaaactaaat cacctggttt ggcatctttt
     4561 agatcaatat gagtcatggc atcatattga tcctgagcca cccgaggaag tttcaggtca
     4621 actttggcat aacaccattg ggtcaagcca ctacaatcaa accccgtttc tggacttgag
     4681 ccaccaaaca cataggccgt tccttcatac ttaaatgcct catcaaaaat ttcttggact
     4741 attttgtcat caaactgagt tgtagttagg tattgcttca ctaataaaca gtaaaacata
     4801 ttgccataat tataccgcca cccaccattt tccttaatcg cgattgggtt ggggtaatcg
     4861 actttttgac cacctgattt ttcctttgaa aaagattcag ctaaagcaaa actatgcttc
     4921 ttcccgtttt tcccgacatg atctagatac ccgccaccat agttataggc ctgaataata
     4981 ctatcttgat cacaccctag ttctttggaa cgcttaacca aagacgcaaa atagagacag
     5041 ccttgtttga tggatgcctc ggtacttaat gaattaggtg gtaatccggc tgactcagaa
     5101 ctttgcataa cgtcttccat tgtgccacca gattccactt gaataatcgc caaaagaatt
     5161 ggaatatact ctgttatatc cgattctttg gcatactttt ccaccattgc acggtgtttc
     5221 aatacttctt ctgagacact agttccgcca ggagaagtgc cagaactacc atttccctca
     5281 ctgtcttcac caaacaacaa acccacacac atcaaaatac ccataaaaga gatggtcaga
     5341 aaagttaatg aaataacacc cactttcttc aagttcattt tttctcacct gaccttgatt
     5401 ttggacgatg gttcattctt ttttgtactt tttgattggt ctgctttggc agagtggacc
     5461 tctgtttcac ttgttggaaa gaatttttct tacttgctga ttggcttttc ccttttgaaa
     5521 tgatttcttg actagttagt tttcgtttga tttcgggatt cagatttgct ctcacaatcc
     5581 gctttggtgc ttcttttttc agcatcatat ctttctgagg ttgagctgct ttatctattg
     5641 gaatattcgc ccttcttttt ccctgacgat ttgttgcttc actcagtatc ttgcgttttt
     5701 cgtccatttt cgcatttctt cgttgtcgtt gtttttctcg caaatccact cgatccgctt
     5761 tttcttggaa cagtccgcgc ttgaattctt ctggtgcttt tttcgacttt tcgattcctt
     5821 tatggagatt gtatttcaaa ttcgttggcg tattccttac ctgatccttt acttgtttag
     5881 tcttatcttt caccaaagct tgggtatcca aaactttgcc gatttttctc ccagccaaat
     5941 tcattcggga ttgtttctta ttatttgaaa ctggattctc cttagttaag tcagatagta
     6001 tttcgtgatc gttcggtaat cgtgaattct ttcgtagaga agaaccggat ggcttaaatt
     6061 tggattttga ttgttccttt gccaacttat ttccgactgc tgcaccacca ccaaaagcca
     6121 aagctcgtcc cacatttcga ttgagttgcc gcaattttcg attcattaac atttgcggtt
     6181 ttcgcatgac gcgtcttccc attgactggc tatcattact ctggaggttg aacatactca
     6241 taatgtcgcc caatttgaaa tagataccgg caaaggtcac aatttgcaga aaagcaacca
     6301 tgaaaaatgg ataggttgcc gaaatattga aaaacatcgt ggagatgcta aatgcagtcg
     6361 taatcaccaa agtaacaccc gctcgcatca taatcgtatt gaataaacga ataatcgcct
     6421 tctttcctaa actttcataa gtcggcaaca tggacaataa gaaactaatt ggcagaaaca
     6481 tcgcaaagat aatgaagaga atttgcgaga acaacataat ccctgttaga agaaaaacaa
     6541 agaaggaaat gccaatattg aacaatacca aaaagaccac ggtccctaaa cggttcatgg
     6601 tcttggtaat actgagattc gcattgtcat tgtcttcaat ctctgccttg accgcttctt
     6661 cccgatcttt gcctttgttt tcatctggac tcacagatag aatcttgttg acccggtctt
     6721 caccaatttc ttctatgttg gaatcatcaa attgcagtag taaccaaggt tgttggactt
     6781 gaatcgaaaa caaactatcc cgaatcaaat ccacactatc ttttccttgg ctttctgaat
     6841 tgggtacaac gatctcggtt ccaagggtaa gggccgcttc actaacatct gagctaaagt
     6901 cattgatttt tgtgatatag gtaggcgcat aagcaatgaa cgaacccgat agtagaaaaa
     6961 tcatcacaaa attaatcacg gctcgaactg ctttcgtggt ttctcgtttt aataatccgg
     7021 tatacgccac atacaccccc agagctaaaa tcatcaataa tagaaaccca aaataaaatc
     7081 cggaggtctg gagtccattt tcagtaatac cagccaatgt ttggatattc tttccaatac
     7141 tttcagccgt atccgaaatg aaatctaatt tgtaagcctc ttgaaccaca taacccgtgg
     7201 cgttcgacaa atacaaactc accgtccaaa tgaaattagt aatggcataa agaccataca
     7261 tcacgctttt ccctaagcca tcaccccaat tccaaggcaa ccaatcccaa gagctatcca
     7321 caaagaaatc tagttgatag ttgttcagtg aatatttgga atatagattt cctgcttcaa
     7381 ctgtatcatc aactagccct gtcgcctcgg ctaccgtacc aactaaactc aatagaacta
     7441 tcagagtgag tatgctaata aaaacgattc ccacaatccg aaaaagtttc tttttcatcc
     7501 cctcactcca ttctctcttg aacaggtgga cgggtatcaa aggcatggaa gagttcttca
     7561 aagactggat gtacctgaac cacgcctacc cgaccataca aatcctgcat caagcattgg
     7621 ccattttcca aatctcgtag gcgtttttga ttgctttcat cttctcgatc caagccaaag
     7681 aattccaagg ttttcttgat ttcatgaata tcggtactgc gaaacgcaaa cttcaacccg
     7741 atattattct tcaagcgttc atccagtaaa tcatcactat tttgcgtgac aaagtaaacc
     7801 ccggcattca tagcccgccc agcacgaacg agcttattcg ataatgtttt cccttgcgct
     7861 acttgaagaa aactccacgc ctcatctaag tctacaattt tgaagatttc tcgattacta
     7921 tggataaagt ccaaggcaaa agtggaaatg acgattagca tggaaacact aagcaattcc
     7981 atggtggtat attcttcaaa agtggtttct gcatctggta aaaccaaatc cgctacttga
     8041 atgatattta actgcctatc taaactaatc gactgggtaa tcgaaccatc tgagaaaagc
     8101 aaatgggcaa aatcataatc ggtaaaggac tcgatgtgat ccgcgatatt ttccgcaact
     8161 ggtgagcctt cttttcgaag ctcattaatt acttgtaata atccccgttg attgctttgg
     8221 gtaaccgtcc gaatggcctt acgcaacact ggaaacttct ccccatctcg actagaaatt
     8281 cctgttaaaa aggtcagaat atcaattgct agactttcag aatccttctt tttctccata
     8341 atcacataag gatcaagtaa tccttgattt tttggcttac tagttaagtt gagaatattg
     8401 atttcatccg caatttctgg caaggtttct ttccagccac cccgttcccc ttttggatcg
     8461 acaatcacag cttgtccacc aaaaaggacg gcgtaataaa ctaagagatt gttactaaag
     8521 gactttccgc caccgagtga accgaggaat gccgctgcta gcgcattggt aactgaatcc
     8581 ttgacccctt gtgaggccaa tgccggtttc aggtaaacat tgcgccccgt atctacgtta
     8641 taaccaaagt aaatgccttc caactcccct aacatctgag tggcgccaaa accaagtccg
     8701 gcgagaaaat cagacgtaac atactggata taatcattca tgtatcgctt actagaaggt
     8761 aagaattctt catgtagccc catcatatcg ccaaaaggcc ggactagttt tacattggta
     8821 tcatcataaa aatctagcac ttcatcacac cggcgcttta gttcatctac tgattcggca
     8881 cttactcgaa ccacatagct cagtttgtac atggattctt tggactgatc caaggtagtt
     8941 tccaattcat ctacggaatc caatgcatct aagacattgg aatttgtttc attgtcagat
     9001 tgataggcat ggttgtccaa gtcttttaat tcctttttct tattgcgaac ggtagcaagt
     9061 gcctttttgt tcgtcacaat ctccacattc atactagtgt caattgggaa tgtaaattgt
     9121 tgttgctggt aatagaaaag ttctgaggag ggaaactcca tttcccccac aattgtatta
     9181 attgttaagt acgctacata tgattcatga ttctcatgtt ccatgcataa gtaccggggc
     9241 ttttcttcaa tcaaacaacg actgggacgt aataagtcgt aacgtttcac caaagtttcc
     9301 gacttcagct ttttctttgg tagctgaaat tcatattctt caaatggtgt acccttttcg
     9361 ccatagatat gttcaatcaa atacatcaga tcactcggtg tgactcgtct gactttaaat
     9421 cttcgagcca atttactttc cattagtttt tcaagcttgg tatatcggcg aatttcttca
     9481 ttggataagg aaacaaaatc gcccatcaaa tgatgattca ctccatagac aaattcttga
     9541 aagccggaga agaaagattt cttgagattc tttagattga cctcttcatc tgtcgcaatc
     9601 agtttgaagc caataaaaaa gcgataatca atctgactat cgccaatcat ggatactaat
     9661 gcctctgtct gtaaatcaat tcgttgtttc gctacttctg ttaatcgccc ggtaatttcc
     9721 ttttttgatc gttcttgcgt ggcacgaata ctactttctg tggcaatttg taaggcatga
     9781 atcttgcctt cacgattttg cgcaatcaat tgacgaaagt tatcgtggac ttgatatttc
     9841 tgttcaggtg acaaaaagga gtaattgtaa ggtattaact catagtaggc aaaacattct
     9901 ccctcttgat taaagacaag attattttca atatacttaa tcgggtacat agttcacact
     9961 ccttaccaga gtaatgggtt cgtcccatcg tttcttctgt tctttgactt tctttcctgc
    10021 gaaagtgacc ttaaatcgta agtgatagct aataaaagat cgtaaaaatc ccaatggttt
    10081 cttaccgtca aacgtttttt gcgacatgaa ccaagtaact gccaccggta tacctaagta
    10141 ttttaaaaag gccccctcta tcatagatag tggaggcaca tgggcaaata cgatgacaaa
    10201 cagcaaggat aaaacaaacc aagccatctg gttaaaagtc acaggaaaag gcagttgaaa
    10261 gtcattaatt gcataaatga ccttttcaac tgcccaaata ctggtgtagc tttttatttt
    10321 cttcatcaaa tcattccttt aaaaagaagg atagtttaga aatcaactac ccttcaataa
    10381 ttttgttaat atggatactc aaaaacacct cgactcgtta ccaaataatt gcctccaatc
    10441 tccaagtctc taccacacgc atcaaaatca atatacgtct tcaagtgttc tggtacttct
    10501 ccaaaatcac cagcttctac aatcaagaaa tatgctacat cactcatatc atcgcaatct
    10561 ggataataga taatatcatc cacatgctct accatctcct caaaagaatt gaagaaagca
    10621 tgttgaatct cagaagcaac ttcaccaact ggcgttcctt caagttcctc cgctaagcca
    10681 cacaaccgat taatttcctc aattggcgta tactcatcaa tctcaaaggg aagttcataa
    10741 tcatgaattg catattcctc gtattcgtca tttaacccaa ttcgttcttt cacttcatca
    10801 aaatcgacag gtggggtaaa ccacgccccc accaattcac cttcgttata tttcccgagg
    10861 ttcgcaatat aaacacgcat ctgttccatc gtgcttcccc cttacgcacc gacaattcgg
    10921 ttaaacaaac tgagtaagac atccttcacc ccagaagcat taaagaccaa tccgaccgca
    10981 atcaaagcaa tcaccaaaaa gccaatcagt ttggaaaact cccgcttaaa tcctaaaaac
    11041 aaaccaatga ccacaattgc cagtaagact aaggattggg cattggttaa aaaccattga
    11101 tatatggata ggtaagttcc tgatattata aatatctcaa acttttcccc ccattcacac
    11161 ggagcgtgcg actttcaccg cactccgcgt tccatcgtgt ttatat
//
LOCUS       DAZKNM010000083        11198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000083 DAZKNM010000000
VERSION     DAZKNM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.084"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8M9_001731"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8M9_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0378494.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8M9_001732"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8M9_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0378495.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG8M9_001733"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG8M9_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0378496.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8M9_001734"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8M9_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0378497.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8M9_001735"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8M9_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0378498.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG8M9_001736"
     CDS             complement(6343..7062)
                     /locus_tag="ACG8M9_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0378499.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG8M9_001737"
     CDS             complement(7059..8372)
                     /locus_tag="ACG8M9_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0378500.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG8M9_001738"
     CDS             complement(8453..8773)
                     /locus_tag="ACG8M9_001738"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0378501.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG8M9_001739"
     CDS             complement(8881..9168)
                     /locus_tag="ACG8M9_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378502.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG8M9_001740"
     CDS             9295..9939
                     /locus_tag="ACG8M9_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0378503.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG8M9_001741"
     CDS             complement(9998..10705)
                     /locus_tag="ACG8M9_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0378504.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG8M9_001742"
     CDS             complement(10925..11095)
                     /locus_tag="ACG8M9_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378505.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAZKNM010000084        11123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000084 DAZKNM010000000
VERSION     DAZKNM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.085"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1344
                     /gene="lysA"
                     /locus_tag="ACG8M9_001743"
     CDS             85..1344
                     /gene="lysA"
                     /locus_tag="ACG8M9_001743"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0378506.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1331..2131
                     /gene="dapF"
                     /locus_tag="ACG8M9_001744"
     CDS             1331..2131
                     /gene="dapF"
                     /locus_tag="ACG8M9_001744"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0378507.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2134..3015
                     /gene="dapA"
                     /locus_tag="ACG8M9_001745"
     CDS             2134..3015
                     /gene="dapA"
                     /locus_tag="ACG8M9_001745"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0378508.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3012..3728
                     /gene="dapB"
                     /locus_tag="ACG8M9_001746"
     CDS             3012..3728
                     /gene="dapB"
                     /locus_tag="ACG8M9_001746"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0378509.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3734..4429
                     /gene="dapD"
                     /locus_tag="ACG8M9_001747"
     CDS             3734..4429
                     /gene="dapD"
                     /locus_tag="ACG8M9_001747"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0378510.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4445..5446
                     /locus_tag="ACG8M9_001748"
     CDS             4445..5446
                     /locus_tag="ACG8M9_001748"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0378511.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5443..6804
                     /locus_tag="ACG8M9_001749"
     CDS             5443..6804
                     /locus_tag="ACG8M9_001749"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0378512.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6764..7915
                     /locus_tag="ACG8M9_001750"
     CDS             6764..7915
                     /locus_tag="ACG8M9_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0378513.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7890..9059
                     /locus_tag="ACG8M9_001751"
     CDS             7890..9059
                     /locus_tag="ACG8M9_001751"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0378514.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9234..10613
                     /locus_tag="ACG8M9_001752"
     CDS             9234..10613
                     /locus_tag="ACG8M9_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0378515.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10894..11123
                     /locus_tag="ACG8M9_001753"
                     /pseudo
     CDS             10894..11123
                     /locus_tag="ACG8M9_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2493 g         2983 t
ORIGIN      
        1 ttaatattat tccaataaaa ttcaaatctt tatcaaatga aattccaata aaaacggaaa
       61 aaaagtgaaa agaggaatga aaaaatgaca gaattgataa ttggcggagt cacagcaagc
      121 acattggcag aaaagtataa gacacctctg tatgtgtatg atgaagaaaa gatgagacaa
      181 acgatgctgt cattcaagcg aggctttgta tcgaaacgtt ttgatacaaa agtgctttac
      241 gcctctaaag cattccaaac gattgagatg ctgcatttag ttcgtgaata tggattcggg
      301 ctagatgttg tcagtggtgg ggaaatctat accgctttgc aagctgattt tcctagagat
      361 actatctact tccatggtaa caacaagacg ccagaggaac tgatctatgc acttgaaaat
      421 gatttgttgc atattgttgc cgataatttg atggaagttg aattattagc agaattaagc
      481 caacgctatt taaaaaaaat ctgtatcatg cttcggttga atgtcggagt agaagcacat
      541 actcatgaat atatcgtcac tgcgcatatt gattcaaaat tcggcatgag ctatgaaagt
      601 aaagagtgcc aacaagccct tcagttgata aaagaaagca gctatctaga gttagaagga
      661 tttcatgctc atatcgggtc acaaattttt gatgttactg cttggttagc tgaaatcgac
      721 aaattgacga attacttaac cgattttgac caacctcttt ccttgaatct tggcggaggg
      781 tttgggattc gttatacaca aaatgatcag ccgatcccag tcgaagaggc actaaaaaaa
      841 ctgattgaat atacggaaga agctttggaa aaacgaaaac tgaccattcg tcaattactg
      901 atcgaaccgg gtagaagtat tgttggagaa gctggtacaa cactatacac gattggtttc
      961 atcaaaaaga caccacataa aaaatattac tttgtagatg gcgggatgac agataatatc
     1021 cgtccagctc tgtatcaggc agaatacgat tgtgatttag cagcaaaact gtcggaagaa
     1081 aaaacagaga aagtgacgat tgcaggaaaa atgtgtgaat caggcgatgt ggtaattaaa
     1141 gaagcttatt tgccgaaagc agaatcgggt gatcttttag ctgtttattc cacgggagct
     1201 tatggatact cgatgagcag caactacaat cgagcgacac gaccagcagt tgtttttgtc
     1261 aataatggcc aatcaagact gattgttcgc agacaagcat tttctgatct actaaggggg
     1321 gaagtttcct atgatccttc ataaatataa tggctgcgga aatgatttta tcttattaga
     1381 ttacgcagaa gacacagatt atagtcaatt agccgcttct ttatgcacaa aagaaaaata
     1441 tgatatagac ggattgatcg ccgtaaaaat cgatccgttg gaaatgatct attacaacaa
     1501 agatggcagc cgcgcgccga tgtgcggaaa tggcattcgc tgttttgctc gatacgtaaa
     1561 tgatcagaac atgatgaatg aacaggaatt caacgtagag acattagcag gcacaagaaa
     1621 aattacggtg ttggatccct caccttttta ttgcagcgta gatatgggag tgccagatga
     1681 ttctccggaa aaaatcaaag ctaatcaaac ttatccaata aaaaaccagg aaatagagat
     1741 caatggtgaa aaagtaacga tcactagttt atttttagga acgattcata ccgttgtttt
     1801 tgttgaaaat gcgcgtgagg aagtctggag aaaaagaggt gaactgatct gtcatcatcc
     1861 tttgttcaaa gaaaaaacca atgtcaactt tgtagaagta gtcaatgaac aagaattgat
     1921 cgtccgtacc tatgaaagag gagtaggttg gacacttgct tgcggtacag gttgctgtgc
     1981 ctcttatgtg gttgctaagg aaactgggaa aatttcagca gatcaggtag ttgttcactt
     2041 ggaacaagga gacttgatga tctctggtga ggatgcgata caaatggctg gcccagcagt
     2101 tcatgaatgg acaagaatat tggaggaatg aagatgataa aaggttcgat cgtggcgctg
     2161 atcacgccga tgaatgaaga gggatcagta gattatgctg gtttggaaaa attgatccaa
     2221 ttccatttag atgaacaaac agatggatta ttagttttag gtacaacagg agaatcttct
     2281 acgctcacac aatctgagga ggagcagatc cttcaactga cagtaaagaa agtggctggc
     2341 cgtgtgccgg tgattgcagg agctggaacg aataatacga aagaaacgat tgaaaaagcg
     2401 aagcattttg cttctttggg ggcagacgca cttttggtca tcacccctta ctacaacaaa
     2461 acaagtgatg caggactagc tgctcatttt acagcaattg cagaagcctc accgattccg
     2521 cttattttgt ataatgttcc ttctagaaca ggcatgtcga ttccaatcca tgtattagta
     2581 aatttagcag aacatcctaa tatcattggt ttgaaagaag cctcaggaga tatggcttat
     2641 gtgatggatg ccgctcggtt gattggagaa gaattcttcc tttacagtgg aaatgatgat
     2701 ttgatcctac cagtaatgag tgtaggagga agcggtgtaa taagtgtatg ggcgaatatc
     2761 caacctaaaa tagtccatga attagtaaaa gatacgcagg acggaagatg gcagcaagca
     2821 aaagaaaagc aattaaatgc acttgaactg atccatgcct tgttcagtga gaccaatcct
     2881 attcctgtca aagcagccat gtcattatta gacttacctt ccggacctct acgcctgcct
     2941 ttagtttctc tttcaaaaga gaaaaagaaa caattagctc agcttttgtt aaaagaaagg
     3001 acggttgaaa aatgaaaatc ggactgattg gcagtggaca gatgggaaat agagtcgaag
     3061 aagttgcaca agaaagagga gatacggtcc ttcttttacg gacggcacct aaagaagcaa
     3121 ctacgcttgc ttttacggaa ctgccagaac tgcttattga tttttctcat ccagataatt
     3181 tagagaaaat cctttcgtat agcttatcat atcaacttcc gttagtcatt ggcacgacag
     3241 gttatacaca atcacagttc caagaaataa aagaacatgc caaacatgta cctgtgatgt
     3301 atagtgctaa tttttcgttc ggcatcatgg tgatgaatca actaatcaag caagcagctg
     3361 caatgttgca aggctggcag atcgaactga ttgaaaaaca tcatagtcgt aaaaaagatg
     3421 caccatctgg tactgcaaat atgctgcttc aaacaatcca agaagtgcaa aagctgcagc
     3481 ccgtatacaa ctggcaaggg aaaaaaagag aagaaaacca aattggtgta cacagtattc
     3541 gtgcaggttc tttacctggt gaacatgatg tattgtttgc tgctactgat gaaatattta
     3601 cgatcaaaca tgaatctttt tctaatcgaa tctttgctgc aggcgcagta gaagcagcgg
     3661 actggttgaa agatcagtca ccaggttatt acaaattaga agatttatta atggacaaag
     3721 gggtgtaaaa aacgtggaag ctcaagaaat catccgctac attcaaacgg catctaaaaa
     3781 gacgcctgtg aaagtctatc tgaatgtaac cgagccaatc gaatttaaag aaagtaaagt
     3841 ttttggtgaa ggaagcagct tcgttgtttt tggtgactat gagacgattg ctccggtttt
     3901 agaagctgaa tcagaaaaaa tcattgaaat cgaaatcgaa actgctgcaa gatacagcgc
     3961 agttcctttg ctagatatta aaaaaattaa tgctcgtatc gaaccaggtg ctattatcag
     4021 agatcaagtg tcaatcggca acaatgccgt gatcatgatg ggagcgatta tcaatattgg
     4081 tgcagtaatc ggtgaaaaca cgatgattga tatgggtgct gtcttaggtg gacgagcgac
     4141 agtgggcaaa aactgccata ttggtgcagg agctgtctta gcaggtgtga tcgaaccagc
     4201 atcggccaaa ccggttatcg tagaagacgg cgtactggta ggtgcgaatg cagtgattgt
     4261 tgaaggtgtc catatcggaa aagatgcagt cgtggcagca ggagcagtgg ttttagaaga
     4321 tgtggcagca gaaactgttg tcggtggaat acctgcacgt gtattaaaaa taagtgatga
     4381 tcagacaaaa gaaaatacag cattgattgc tgcgttacgc gaattataga ggagatggag
     4441 aaaaatgcgc gaaatagata cctgtattgt gggggcaacc ggattagtcg gccaaacgat
     4501 gctcaaagtg ttagaagaat atgattttcc agtaggacga ttgaaattat tagcttcaaa
     4561 acgttcagcc ggtaaagttt gtgtattcaa gaaaaagaaa cacactgtac aggaattgac
     4621 agaaacttca tttgaaggat atgatttagc gctattttct gcaggtgctt ctgtatccaa
     4681 agagtttgcg ccaatcgcac gaagtcaggg actatttgtc atagataatt cctcagcatg
     4741 gcgggaagat tcggatattc cattaatcgt tccagaggtc aatctggaag actatacatt
     4801 gaatcggctg attgcaaatc caaattgttc aaccattcaa gcggtacttc cgcttaaagc
     4861 tttacaagaa gcctttggta tccagcgagt caattattcg acctatcaag cagtttctgg
     4921 ttccgggcaa aaaggcattg aagatcttca tcgtacgcaa aatgacgagg aaccagtttt
     4981 gtatccttat aatattagcc aaacggtgat tccggaaatc gatcagcctt tggaaaatgg
     5041 gtatacaaaa gaagaacaga aaatgataaa tgagacccgt aagattctgc atcagcctgc
     5101 tttgccagta tcagcaacct gcgtacgtgt gccaatcgaa aatggacatg gagtctctgt
     5161 ggcagttcaa ctgcaaaaag agtttacgac ggaagaaatt agagagtgtc tcagccagtt
     5221 tccagggatt caattagaag atgatctagc agcacataaa tatccgacga gcattcttgc
     5281 tagaggaaca gatatggttt atgttgggag gatcagaaaa gacacttctt tagagaacgg
     5341 attgttgttc tatacgactg ccgataacat acgcaaagga gccgctgcga atgccgtaca
     5401 gattgctgca gctttaatgc agaaaatagc ggaggtggca agatgagtaa tacgctgaaa
     5461 gtagcgaaat tcggcggaac atcgatgtct catgaagagc aattcaaaaa agtccgtaca
     5521 attgttcttt cagatcccaa tcgccaagtg atcgttgttt ctgctttagg caaacgtacg
     5581 tcagatgatg acaaagtaac agacttactt tatttgattt atgcttacct tcagcatcat
     5641 gttgattgga cacccctttg gaaaaggatt tgtgatcgtt tcattcaagt aagagatgtt
     5701 cttgccctgc agttacctat tgaagatgaa ctagcacgca tacagaaaca actggaagac
     5761 gaaaagataa ctgaagatta tttagtaagt agaggtgaat atctgactgc tcaactgatg
     5821 gctgagtatc ttggttacca gtttattgac gcaaaagacc tgattttctt tgactatgat
     5881 gggaaaatcg atcaggaaaa aacagcacag gaaatcacaa aagcatttaa aagatatccg
     5941 aaaatggttg ttccagggtt ttacggggcg aaccctgcag gaaaagaaaa attattgggc
     6001 cgtgggggaa gcgacattac cggcgcgata ctggcgaaga tgttgcaagc ggaaagttat
     6061 gagaattgga cggatgtttc aggaatcatg atggctgatc cgcgaatcat cgatcatcct
     6121 aaaatcatcg aggaaatctc ttttagagaa ctgagagaga tggcttatat gggggctaac
     6181 gttcttcatg aagcagctgt tttcccagtt caagaggctg atatccccat ccaaataaaa
     6241 aataccaatg atcctttcgc tcaagggaca aaaatatcga atcacgaaat agaaaaggaa
     6301 aatggactga caggaatagc tggacgcaag gattttcttt ccatcacttt attcaaacgt
     6361 catatgtccg atgaaatcgg gtttatctgg aaagcaatga gcatttttgc taaacatgag
     6421 atatcaatcg aacatattcc ttcagggatc gacaatatcg gagtgatcgt ctcagcagaa
     6481 gcgattgccg ataagttatt tctcatcacg aaagaattaa aagaaacact tggcgtggaa
     6541 gaaatcgtcg tgatcgaaga cttagctttg atctctgtcg ttgggggtcc gcataaagaa
     6601 ttgattggat tatctgggaa agtgttatcg atattgaaca aattagaaat tcgcacatcg
     6661 atcttatcac aaggagcaca ggagttgaat ctgatcatcg gtgtaccgaa taatcaatat
     6721 gaaacagtag tgaagggtat ctatgaaggg atggtgaata cggatgctga aagaatatcg
     6781 aatggcactc catcaaattc ctgaaatcgg gtttcatgaa ttcaagacaa aaaaatattt
     6841 atatgaacaa gtaaaaaaca gcgaggccgt tatccatgaa atcggcgaaa caggactact
     6901 cctttatttt gataatcagc aagaagaaac gattgctttt cgaacagata tcgatgcact
     6961 gccagtcaaa gaagaaacga atctctcttt tgcttctaag catgaaggtt atatgcatgc
     7021 atgtggtcat gacggacata tggcgatgct tttagggttg actgacttta taactaaaca
     7081 cattgaggat cttatttata atgtagtact gatctatcag ccatcagaag aaatttccgg
     7141 tggcgcacaa agcgttattc agtcaggatt gttggataaa tatgaagtac aagcaatatt
     7201 tggctttcat ctatggcctg gactagctaa gggagaagtt ttctctcgac caggaccttt
     7261 gatggcacaa agcagtgaaa cagacattat cattaaaggc cagtctgcac atattgcttc
     7321 tagtgaaaaa ggaatcgata gtctggaggc agctgtacga tttttgaaga atgtttatga
     7381 ttttgaccgg caattaccgg cagagtggaa tcatctaatg aaatttggcc aaatccaagg
     7441 tggaacgatc cgaaatgttt tagctggaga agttattgta tccggaagta tccgttctta
     7501 cagttcagat atgcaaaatt atttgaagag gggattagac catttagcag aacagtttca
     7561 ctctacagct actatttctt ttcgctataa tgacggctat ccagctgtaa tcaaccatag
     7621 aggcttatat gcagcactgt tagattcaaa caaactacat gaattagatg ctccagttct
     7681 tcaagccgaa gattttggca tatatacgca acaatatcca tgtgtatttt tctttttagg
     7741 tgtcggtgat acccctgctt tacatgatcc gacgttcgat tttgatatgg atgttttaga
     7801 aaatggtgta gaatggtaca aaacgattct ttttacagaa aatcttcttg taaaaatcga
     7861 tagcagttca aaagaggagc gagtaaataa tgatgaattt atccaatcga tttagtccta
     7921 ggctttctag aatagaagta tcaaaaatac gacaattcga tcagcaaata tcgtcgatac
     7981 cagatgtgat caaactcacc ctgggagaac cggattttcc aactcctgaa catgtcaagc
     8041 aagcaggaat agcagcgatt gaagaagact tttctcacta taccggaatg agaggattag
     8101 aggaactgcg ggaagcggct tgtatatttc agcaacagcg ttatggatta acttatgatc
     8161 cgcaaacgga agttttgacg acagttggtg caacggaagc gattgcctct gcgttgttat
     8221 ctgttttgga agaaggagac aaggttctta ttccagcgcc tgcttattca ggatatcagc
     8281 cattagtgga actagctggt gctgaattga tcccgataga tacttctgac acagggtttg
     8341 tctgtcagcc ggaacaattt gaacgagcat ttgaacaata tggatcagct gtcaaagcag
     8401 tcattttaaa ctatcctaac aatcctactg ggacgacatt gtctgcaaaa caattgaaag
     8461 caatcgctga ggttttaaaa aaatatccgg tatttgttat aagtgatgaa gtatatgctg
     8521 aattgactta tagtggaacg catatgtcca tagctactta tcttccagaa caaacaatcg
     8581 tgataagcgg gttatccaag tcacatgcga tgactggttg gcgagtgggc tttatttttg
     8641 ctcaaaaagc ccttattgac gaactaatta aagtccatca atatctggtg actgccgcta
     8701 caacgatgtc gcaaaaagct gcggctgaag cgctaataaa tggggtgaaa gatagcgaga
     8761 atatgaaaga gcaatatcaa acacgaagag actatctggt ccagcaattg gcgcctttag
     8821 gatttgaagt gacccagcca aacggtgcat tttacttgtt ttgcaaactc ccagctgcca
     8881 ttcaaactga ctcttggcag ttttgtgtag aattagcgga acaggcaaaa gttgcgtgta
     8941 tcccaggaat tgcatttggc ccagaaggag aaggttatat ccggatttct tatgctagca
     9001 gcatggaaaa attacatgag gcctgcaaac ggatcaaaga ctttttaaca gaacagtgat
     9061 tcatagattt tatcaaaaaa acaatcgtta attttgcggt atattctgca aggagacgat
     9121 tgttttttaa atttagttca aatgaacgcg tttcttttga ttcataacta atgtaatgtt
     9181 aagtttaaat tgtttagaat gattttaaaa gaatattcaa ggaggtttca tcaatggaaa
     9241 aaatcaaagt tgtcattgta ggtgcatctc atggagggca tcaatcgatc ttggaactat
     9301 tgacacgata cggtgaaaat gtagatatca cgctatttga agcaggagac tatatttcct
     9361 tcatgtcatg tggtatggag ctgtatttag aagacaaggt aacagatgta aatgatgtac
     9421 gtaatttcag accagaaaac tttccgcaat ccaatgttca tattttaaat aatcacgaag
     9481 taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca gataacgaaa
     9541 caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca aacaatctgc
     9601 cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat tgggcaacca
     9661 aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta ggtgcgggtt
     9721 acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc gtattgcttg
     9781 atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat attttagaac
     9841 aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa gcttttactg
     9901 gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca gatctagtca
     9961 ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc gacttagatg
    10021 aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat gtgtatgcag
    10081 taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaatgccg atcgctttag
    10141 cgactgttgc acgtagagaa gcacgttatg tagtaaaaca tctttttgaa gaagtaccaa
    10201 acaaaccgtt cggcggagtc gtaggttcct ctgcattaag tgtctttgac tatcattttg
    10261 cagcaagcgg attgaatagc tttacagcag caaaagcagg agtagatgta cgagtgtctt
    10321 attatgaaga tacgttacga ccaaaatatg taccagaaga acaaggaaat ccaaaagtgt
    10381 ccatccaatt attctcggat ccaatgaccc atcaattgct aggaggagca gtattatcta
    10441 cctatgatgt tacaggtcaa ggaaatgtat tggcactagc gatccaaaac aaactgactt
    10501 tagaagaact agcagaagct gacttcttct tccagccagg ctttgaccga caatggagca
    10561 tgttgaatct agccgctcaa caagcgttag gagaagaacc atttgtagaa taagaaaacg
    10621 aaatatttga aaagaggttg tgaaaaagct gccttgcatc aagtaataag aacagccaag
    10681 taaaaacagc ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt
    10741 gaacaccgtt tattttcaaa aaagggactg tgacacgatt tgtgtaccga cccccaaaag
    10801 ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggttaaat atacttttga
    10861 acttaaatta aagatcgttc atgattattt ggatggcaaa ggcgattgga ccgtaaaaat
    10921 ccagatcaag agattgaagc ggaaatgctt aaaattcggg agaagcacaa ggattatggc
    10981 tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa aaagaaagtt
    11041 cagcgcttga tcaagaagct aggaataaaa gtaacggcat acacgcgtaa atcacgtcgc
    11101 tacaattctt atcgaggaaa agt
//
LOCUS       DAZKNM010000085        11089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000085 DAZKNM010000000
VERSION     DAZKNM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.086"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..1134
                     /gene="pplA"
                     /locus_tag="ACG8M9_001754"
     CDS             199..1134
                     /gene="pplA"
                     /locus_tag="ACG8M9_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0378516.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1224..2327
                     /locus_tag="ACG8M9_001755"
     CDS             1224..2327
                     /locus_tag="ACG8M9_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0378517.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2340..3293
                     /gene="menA"
                     /locus_tag="ACG8M9_001756"
     CDS             2340..3293
                     /gene="menA"
                     /locus_tag="ACG8M9_001756"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0378518.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4107..5453)
                     /gene="gor"
                     /locus_tag="ACG8M9_001757"
     CDS             complement(4107..5453)
                     /gene="gor"
                     /locus_tag="ACG8M9_001757"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0378519.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5587..6840)
                     /locus_tag="ACG8M9_001758"
     CDS             complement(5587..6840)
                     /locus_tag="ACG8M9_001758"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0378520.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6846..7769)
                     /locus_tag="ACG8M9_001759"
     CDS             complement(6846..7769)
                     /locus_tag="ACG8M9_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0378521.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(7990..10482)
                     /locus_tag="ACG8M9_001760"
     CDS             complement(7990..10482)
                     /locus_tag="ACG8M9_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0378522.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10489..10956)
                     /locus_tag="ACG8M9_001761"
     CDS             complement(10489..10956)
                     /locus_tag="ACG8M9_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0378523.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3266 a         2265 c         1942 g         3616 t
ORIGIN      
        1 cttttgtcta actccttttg gatttttttc aaattttaaa ataaagcgtt ttttcacgaa
       61 atttgctgaa agaaaacgtt tgaaatatta catgggttat ttatcaaaaa tagttgactt
      121 ataggctaaa aagattagaa tgtataatga atcacaaaga aaaattgtgg actagaaatg
      181 gaatggtggg gtattaaaat gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca
      241 ttttcagctc tagtgttagg cgcttgcggc gcagatgaca acggaagctc aaactcttct
      301 tctgaatcat ctacagcaca atcttcgact gcaaaaagca caacagaatc atctgcaaaa
      361 gttgtagctg gcggagattt gcaagacggc acatataaat tagaagaaaa aaattattca
      421 aatggttacc gtgcagtctt tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa
      481 tatgataacg tcaatgaaaa cggcgaatca aaaacaaaag atgcagaata caacaaaaac
      541 atggaagcaa aatctggcac aaacccagaa aaatacattc ctgagttgaa tgaccaattc
      601 ttaaaagcac aaagcgcaag cggtgtggaa gtcgtaactg gagctactca ctcatctgaa
      661 agcttccaaa actacgcaca acaattgatc caagctgctc aagctggtaa cacagataca
      721 atcgaaatcg acaatggtgc tgatttgaaa gacggcactt acaagcttga agaaaagaac
      781 tactctaacg gctaccgtgt gcaattcgaa atgacagttg ctggcggcaa agtaactgaa
      841 tcaaactttg attatatcga caaagacggt aaatcaaaac aagatgatac agaatataat
      901 gaaaatatga aagcaaaaag cggtacagaa ccaaaaactt acatccctac tctgaacgac
      961 gaattcgtca aagcaatggg cgaagaagat ggctcacctg ctaatgtaga agttgttaca
     1021 ggtgctacac acagctcaca ttcattcatc atgtacgcac aacaattagt gaatgcagct
     1081 gaaaaaggcg acactcaaac aatcgaagta gataatatcg taacagaaaa ataatcacat
     1141 aatcatacta ataaaagttg gagggtggga cgatagccag attaggcagt cgtctcacct
     1201 ttcaaaatga agagaggaaa aagatgcgga aaaaactact attttcctta atcacactgg
     1261 gcatctcttt tatggtgcta actggctgca gcaactcagc aaataaaaca accactgaaa
     1321 gttcagcagc taccaagata ttgaaagatc catattctga tgaacagttc ttgttaggga
     1381 cttacgttcg tatccgtgtg tatgatgaag gcaaagaatc agcattgaaa cctgccttcg
     1441 atcgagtaaa agaacttggt gataaaatca cgatcaatca aaaaggttct gagatcgatg
     1501 aagtgaatga gcaagctggg atcaaaccag taaaagtttc tgatgacgtc tacacgttag
     1561 tcaagcgggc ctacgaatat agccaagatt ctcaaggcgg attcgatatg gcgattggtg
     1621 caatcacaca attatggcga atcggttttg atgacgccag aaaaccttcc caagaagaaa
     1681 tcgatcaggc attgaaatta gtggattatc ataagatcga actaaatgac aaagaaaaaa
     1741 ctgtttatct aaaagaaaaa ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca
     1801 ttacggacga agtagtgaaa gtcttgaaaa aacaaggagt gaccactgcc atcgttgact
     1861 tgggcggtaa tgtctatgtc ttaggccaca gcccaagagg cgaaaaccaa gactggacag
     1921 tcggcatcca agatccgaac atggctcgcg gatctgtttt aggttctatt aaagaacgga
     1981 acaaaacatt agttacctct ggaatttacg agcgttattt agaagtagat ggcaaaaaat
     2041 accatcattt gtttgatcca aaaacgggtt atccgttcga caatgatatc gcaagtgtca
     2101 cgatcataac agataaatca attgatggtg acgggttgtc tacagcagtc ttttcaatgg
     2161 gtgtgaaaaa aggactggaa tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct
     2221 tcgtgacaaa agacgataaa gtatatgtga ccgatccaat caaagacacc ttcaaattat
     2281 cggaggactc ccattacacg atgggtgacc gttctgaact aaaataagga gtacgaacca
     2341 tgacactaaa aatcttttta gaagtagttg aaatacgaac aaaggtggct agtgtatttc
     2401 cttttgcggt aggggtatta ttctctatcg ctttttttca tgagatacat tggctaaaca
     2461 cttttttatt ttttattgga atgctagttt ttgatttagc gactaccgct atcaataatt
     2521 acatggatta tgaaaaggct cattctgaag tatataagta tgaagaaaat gtcattggcc
     2581 gcagaaatct atctcctgta ttgattcgaa acatgatttt agctatgatt gcatttgttt
     2641 ttgttatcgg cgttattcta acgatcaata cgggttggct tttacttttg atgggattta
     2701 tctgttgttt tattggagta ttctatacat tcggaccgat tcctttatct cgaatgcctt
     2761 taggggaaat tttcagtggg gtgactatgg gccttggaat ttttgccatg gtcgtttatg
     2821 tcaatacatt cggttcagat gtctatgatc tgacagtcaa cttttcatct ggacaatttg
     2881 ctttggtagg aaatctttgg gctatagcag caatcgtatt agcttcctta cccttggtgt
     2941 ttacgattgc caatatcatg ttggctaata atttgcggga tcttgaccgt gatattgaaa
     3001 atcatcgcta tacactagtt ttttatatcg gacgtcctgc gggaacagtc ttattccagc
     3061 tattgatgta tgcttgctat ttcgtgattt taatggggtt gttgagcggt atctatgaat
     3121 ggccgatctt ggtcaccttc ttgaccttgc caattgtgaa gaagcatttg gatcaattca
     3181 agcaaacgtt accccagcct atcagtttca gctattccat caaaaatatg gtcttattca
     3241 atgccagtta tgtattgggg ctattactaa caatcattat gcaagccatt taacagcatg
     3301 aagaggttgt gaaaaaactg ccctgcatca agtaataaga acagccaaat aaaaatagct
     3361 ccttatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     3421 atcaggaggt tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg
     3481 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt
     3541 ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt agagaaacaa atggaaaaat
     3601 agttcctcat attttgccgt tttttgagct aataccgaag gacagctgat tgaacactat
     3661 ttgttcggaa aaagtgacta actatgacac aatttttttc acagtccctt ttttcacttg
     3721 atttgattcg taccagcgaa cttgacgaga actttcttgg tttggtaact atatggtatt
     3781 cttttaacat cctcgtttgc ttcttctaat ttatttatac aaagtcacac ttttttaaac
     3841 atctgttctt cgatacattt ctatttcttt taaacatcga taccaatatt ttgaacttaa
     3901 gagtgcatac caaatggatt catttactct tcgtcagtta cgaagataat caaaatctcc
     3961 aacttcccta cttatttcgt tttgagaatt atcgaagcaa tcttacataa ataactaaaa
     4021 aacaaaaaac cagaaaatca gtatactgat tttctggttt tacatggttt gttcaataaa
     4081 aagctgttgg aactgttaat attaaattat cgcatcgtta cgaattcttc cgctcctgtt
     4141 ggatggatgg ctactgtatt gtcaaagtct tctttagttg cacccatctt gatagccacg
     4201 gcaaaacctt gcagcatctc atcgacacca acaccaatag catgtaagcc gacgattctt
     4261 tcttcttttc cgacacagat caatttcatt tcgcattttt ggcgataatc atttaatgca
     4321 aagtacattg gggtaaagcg tgaacgataa actttcaatt cattttcgcc atatttttcc
     4381 aatgcttctt cttctgtcaa accaattgtt gcaattggtg gatgcgtaaa gacgactgtc
     4441 ggaacaagtt tatagtcaag ataagaatct ttctttccat taaataaacg ttctgataaa
     4501 cgtctaccag ctgcgattgc aacaggagtc agatctaatt taccgatcac atcaccaaca
     4561 gcaaaaatat gttcatccgt tgtattttgg tatttatcta ctttgataaa cccttttttg
     4621 tccaattcca catttgtatt ttccagacct aatttttcgg tattcggtac acgtccaccg
     4681 gcaaaaagta cacaatcacc agttaaggtt tcaccatttt caaatagcac cgtatattcg
     4741 tcattgtttt tagtaatttc tttagcaata tgatgtgcgt aagtttttat tccagcttct
     4801 tggtacattt ggatcaaatt gtccgaaagc attttatcaa acgttctaag tggtcgttct
     4861 tttcgatatg cccacatgac ttcactacct aaaccacgaa atacacctga aagttcagcc
     4921 gcaatgtatc cagcacctaa aacgattgct ctcttaggta actctgttaa ttcaaaaaat
     4981 ccatttgaat caattgcgta ttctccacca ggaatggtca ttttacttgc acggcctcca
     5041 gtagcgatca agattttagg agcacgatag gtttcaccgt ttacttcgat cgtttgacta
     5101 tcgataaaag tagcgtaacc ttttatagcc tcgacatgat tgctatctaa tcctctttga
     5161 taagcaccgt gtagaaaatc aatatatttt tctcgtcttt ctactagacc ggcgaaatca
     5221 acatttttta aatctgcttg gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa
     5281 atcgtacttg cttgccacat aactttttta ggtacacaac cgacgttgac acatgttccg
     5341 cccaattcat tcgcttcaat cagcaaaact tttgcgccat gcatccctgc tctgtttgct
     5401 gaagcaatac cgccagagcc gccaccaatt acgatgtaat catatatttg cattttgttc
     5461 ctccttattg aagagatcaa tcaaaacctt tatttaacga tcctttttct acagaaaaca
     5521 agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt aaaacaggga tatatcagct
     5581 ttttccttat ttacgttttc ggatcatatc tccaggtttc accggctgtg cgactggcat
     5641 agttaaaatc atcattggat ttggtgctcg gtcaatggat tcgccttctt cattatgcat
     5701 aacttcaacg gtttgactga aatgactgaa tcctggacca taaaattcga tttcatctcc
     5761 tacagaaaac aagtttctct gacgtatcgt agcaatctgt gttgcctcat cataagataa
     5821 tacttcaccg acaaatttat atactgggat tttacgacgt tcaccgaata attgttcgtt
     5881 ttcagttggt gtattgtagt aaaaacctgt agctaattct cgttgtgcta ctttccataa
     5941 ctcgtcgatc cattcttgct tgcagacata gttttcagga tcttccatat aactgtctac
     6001 agccgcttta tagacatttg acaccgtaga tacataatgg atcgatttca ttcttccttc
     6061 gatcttgaag ctgtctacgc cgttttcaat caattctgga atatgttcga tcattgacat
     6121 atccactgca ctcatcgaga attcttcttc cacttcgcct gtatcagtta aactgtttcg
     6181 ttcttgacca aaaggcatat cgaacaatcc atacttccag cgacaagatt gagaacatcc
     6241 gccacggttt gcatcccgca tcgacatatg gttggacaat gtgcatcgac cagaataaga
     6301 gatacacatg gcaccatgga taaatgcttc gatttcggta tccgtatttt tacgaatctc
     6361 tgccacttcc tccattgaaa cttcacgagc taaaacgaca cgttccaacc cttcttcttt
     6421 ccagaattcc aatgtctcaa aattagttgc tgaagcttga gtggataaat ggatcggcag
     6481 acctggggct tctgtcgcac aaatttcgat caaagctgga tcagaaacaa tcactgcaga
     6541 aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg
     6601 ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc
     6661 ttcagccatt tcttcataag taaaattacc tgcacggctt cttaaaccat atgcattccc
     6721 gccaatatat actgcatctg ctccataatg gatcgcggtt tttaactttt ccagtgttcc
     6781 tgctggcgct aggatttctg gtcgtttcaa ttttcttttt actgtcattt ctttcatcat
     6841 actccttatt tgatagcaga aggatcaata taaaagaatc ctgtatctaa ttcacggttt
     6901 tcaggatgca atttactgat ttcgctagtc attttttccg ctttttcttg atcccagcta
     6961 ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag tgatgtttac gaatttttcg
     7021 cctggagtaa ataatccgtc caatttccat gtacagtaat gatgctgata aagttcattc
     7081 aattcattcg agagattcag atcattgctg gcaaaaatat gtgtgccatg actatcttca
     7141 taaatagaat aatgtgtctc ttctttttta ggttccgaga taaaaagacc tctttcacga
     7201 tctttttgtt cgtcttgttt cgtatagtta tagtaattct gtagcagtgg acgtttggat
     7261 tgatggatgc atgtagcacc atacacaagg gtttctacag gaatatcgag tttttcttcc
     7321 atcgccttca tctcttcaaa aggaacttca cgagcaagta ctgctccaac tgcgcctttt
     7381 ttcgcccaga aattgatttg acgtgcgctc gttactaatg tttcagcatc ataaatatac
     7441 ggtatacgca attcaggatt ttttttcatc gtataaataa tacctgtatc accaactgtg
     7501 atcacatcta cgtttatttc ttttaaaaaa gccaaatatt caggtacatt tttcattttt
     7561 tcaggatgaa tgatcccatt tacagcaata ttcgcttttt taccatactt gtgtgctaat
     7621 tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc
     7681 tcttcaccga aatatagtgt atctgcacct gcagcaagca actgttctgc ttgttcaata
     7741 gtttctactg tggttatgag ttcgatcata attttctcct cctacaactt cataatagta
     7801 gcatagctct aaaaactttc caatacttct tctatttaaa gtcggatttt tgaaaattat
     7861 ttcacaaaaa atcaaaataa agttcgatat attctgtcca aatataaaaa gagattgtga
     7921 caaaaatctt gtcacaacct cttaatcaag tcttactatt ataaaaattc agctacacag
     7981 gccaagtaac tatagggaac ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat
     8041 cttacctttt gaagcaccta ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa
     8101 agcctcgctc aatcgatctt caatttcttt ttgaagtgcg cgacggattg gacgtgcacc
     8161 atattcagga tcaaatccag ctttcccgat cacatcgatt gcagatggtg tgattttcac
     8221 ttgaatatct tgttctttca atcgtttgat aatcgctttg ctcatgattt ggacgattgt
     8281 atggatttca tctttactca atgaatggaa gacgacagtc tcatcgattc gattcaagaa
     8341 ttcaggtcgg aaagctttct ttaactcttc taagatacgt ttttgcatgg ctttgtgatc
     8401 tttagtaaca tcttggacat tgaatccgac atttttttct tctcgaattt gtgtcgctcc
     8461 gatatttgaa gtcataatca tgattgtatt tctaaagtcc acttttctac ctttagaatc
     8521 tgttaaatga ccatcatcca atacttgcag aagtaaattg aaaacgtctg gatgtgcttt
     8581 ctccacttca tctaagagaa tcactgaata tggtttttga cgaatttttt ctgtcaactg
     8641 tccaccttca tcatacccta cgtatcctgg aggagacccg attaatcggc ttgtactgta
     8701 tttttccata aattctgaca tatcaacacg gatcagggca tcttcacttc caaacatgac
     8761 ttctgaaagt gctttggcca attctgtttt tccgacacca gttggtccaa ggaacatgaa
     8821 tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga
     8881 aactgctttg acagcctcat cttgaccaac gacacgttca tgcagaagtc cttccagttc
     8941 taacaagcgt tcgctttctt ttttctccaa ctgctgtaga gggacaccag tccactccga
     9001 tacaactgta gcaacatctt cttctgttac acgatcagaa taaccttgct cctgttttgc
     9061 ttcaattgca gaaacttttt gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg
     9121 agcagcattt tcaaagtctt gtttttggat cgctgcttcc ttttcttgta ctagttcatc
     9181 gatttctagt ttgatcacag ttgattttgt taagtgatcg gtttgatcca aacggacttt
     9241 cgctgctgat tcatcgatca aatcaattgc tttatcgggt agttgacgag aagtgatata
     9301 acgaacagaa agattgactg ctgctcgtac agcttcatct gtgatctcca ctccatggtg
     9361 ctcttcatat cttgtacgta atccttgaag aatcacttct gcttcttcag gagttggctc
     9421 atctacttga atgcgagcaa aacgtcgctc taatgcagaa tctttttcga tatatttttg
     9481 atattcatct agggtcgttg ctccgatcgt ttgtaattct cctcgagcta acgccggttt
     9541 caagatattc gaagcatcaa ttgctccttc tgctccacct gcaccaatta aggtatgcaa
     9601 ttcatcaatg aataagatta cttgaccatc attatagatt tcgtcgatga cctttttcat
     9661 tcgatcttca aattcaccgc gatatttcgt accagcaacc aaagatccca tatcaagcat
     9721 catcaggcgc ttttcttgca tgtcttctgg cacttcacct aaaatgattt tttgagctaa
     9781 cccttcagca atggctgttt tgcctacacc aggttcaccc accagtacag ggttattctt
     9841 tgtccgtcgg ctaagaatct ggataagccg tttcacttct ctcgaacgac caacaactgg
     9901 gtccagcttg ttttcttttg ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg
     9961 tgttccttgc ggtgtttgcc gtttatcttg tacatttaca tttctacgac gattcattcc
    10021 ttttccagca gaatcaccag ctagccccat tttttgtttc agaacttgac gggcttttga
    10081 aaggctgata cctaagttga tcatgattcg agtagccatg atactttcat cttttaataa
    10141 gctcatcaaa atatgttctg ttccaacttt ttgagaacca gttttcttca cttcgctgct
    10201 agctaaagca aaaacttgtt tcattcttgg tgaatatgga agaaagactc ctttagggta
    10261 agaacggata ccaccataac caatcagatg ttcaatttct tcataaattt ccgtttcatt
    10321 caaatccatt tcacgtaata cttttccagc aattccattt tgctcgacca ctaaggctag
    10381 taaaacatgt tctgaactaa ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat
    10441 ttctaatact gcttgggctc tttcagtaaa aagttcttcc atctaatctc acttcccatc
    10501 ttcataactt aaacgttcca gaaatgctcg gagaatccga gcacgtgtat gatcttcata
    10561 ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac atcaagtttc cttctttttt
    10621 tgtaattatt tggtcttcat aaagtttttg aataatagca aaagcatttt tctcactgat
    10681 tgtatcatca aataactgat ttatctgctc aagtaattgt tttttatctg aaatccttac
    10741 ttttgcaatt cgtatataac caccaccacc acgtttgctt tctactagat aaccacgttg
    10801 gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa ggaacacagt taaagaggtc
    10861 tgccatttca atccttcgaa tctcgatcat ttcactattt tccagaattt tctttatata
    10921 tgattcaatt aaatccgagg tattctgatg tgccatctta ttcattccct ttgactaata
    10981 ttgaccttaa ttataacgaa tttgataata gaaagaaaga agaacgcttt ttgttaaata
    11041 aatcctttag aatcaacaaa aaaaaatacg gaatatacta ttccgtatc
//
LOCUS       DAZKNM010000086        11053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000086 DAZKNM010000000
VERSION     DAZKNM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.087"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..454
                     /locus_tag="ACG8M9_001762"
     CDS             137..454
                     /locus_tag="ACG8M9_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378524.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            387..866
                     /locus_tag="ACG8M9_001763"
     CDS             387..866
                     /locus_tag="ACG8M9_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0378525.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            883..1059
                     /locus_tag="ACG8M9_001764"
     CDS             883..1059
                     /locus_tag="ACG8M9_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378526.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1316..1747
                     /locus_tag="ACG8M9_001765"
     CDS             1316..1747
                     /locus_tag="ACG8M9_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378527.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            1753..2070
                     /locus_tag="ACG8M9_001766"
     CDS             1753..2070
                     /locus_tag="ACG8M9_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0378528.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2189..2875
                     /locus_tag="ACG8M9_001767"
     CDS             2189..2875
                     /locus_tag="ACG8M9_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0378529.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            2850..4199
                     /locus_tag="ACG8M9_001768"
     CDS             2850..4199
                     /locus_tag="ACG8M9_001768"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0378530.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4190..4663
                     /locus_tag="ACG8M9_001769"
     CDS             4190..4663
                     /locus_tag="ACG8M9_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0378531.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            4665..5165
                     /locus_tag="ACG8M9_001770"
     CDS             4665..5165
                     /locus_tag="ACG8M9_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0378532.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            5180..7492
                     /locus_tag="ACG8M9_001771"
     CDS             5180..7492
                     /locus_tag="ACG8M9_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0378533.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            7739..8056
                     /locus_tag="ACG8M9_001772"
     CDS             7739..8056
                     /locus_tag="ACG8M9_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0378534.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            8053..8214
                     /locus_tag="ACG8M9_001773"
     CDS             8053..8214
                     /locus_tag="ACG8M9_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0378535.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            8217..8516
                     /locus_tag="ACG8M9_001774"
     CDS             8217..8516
                     /locus_tag="ACG8M9_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378536.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            8516..8830
                     /locus_tag="ACG8M9_001775"
     CDS             8516..8830
                     /locus_tag="ACG8M9_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0378537.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            8823..9353
                     /locus_tag="ACG8M9_001776"
     CDS             8823..9353
                     /locus_tag="ACG8M9_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0378538.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            9350..9619
                     /locus_tag="ACG8M9_001777"
     CDS             9350..9619
                     /locus_tag="ACG8M9_001777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378539.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            9549..9752
                     /locus_tag="ACG8M9_001778"
     CDS             9549..9752
                     /locus_tag="ACG8M9_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0378540.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            9749..10036
                     /locus_tag="ACG8M9_001779"
     CDS             9749..10036
                     /locus_tag="ACG8M9_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378541.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(10069..10320)
                     /locus_tag="ACG8M9_001780"
     CDS             complement(10069..10320)
                     /locus_tag="ACG8M9_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378542.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            10523..10936
                     /locus_tag="ACG8M9_001781"
     CDS             10523..10936
                     /locus_tag="ACG8M9_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0378543.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         3983 a         1803 c         2480 g         2787 t
ORIGIN      
        1 caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa aaaagacacg
       61 aacgccggca agcatgatcg tgtcagggaa ataaactatc tttctatatt ttaccacaga
      121 aaggaatgaa ccgtaaatgc taattgcaac ggatacacta gaccgcatct ttttaaaaga
      181 cgaatacaaa ctgcgcaata tagatgcgtc aggaatttta gtatttgatc tttatgacaa
      241 tggaaaaatt ggtatctatc aagcaagtga tattgaagaa acaaaccttg ctttcgaaca
      301 aattgatgat tctgtggaat tggatttaga tgaggcaatc ctagcttttg aacaaattgc
      361 aaaattatta aaggaggcac aaaagaatgg caactcttta ccaactcagc gagtcatata
      421 tcaaagtcct ggaactggca gaacaattgg atgaagaaat tcttcgcgat actctcgatt
      481 cgattaatga agcgatcgag tataaggcag aaaacttagc aaaaatagtt aaagaagtag
      541 aagggaaagc tgagttaatc gatagtgaaa ttaaacgtct gcaggaacgt aagacatcgc
      601 ttttgaacaa tgctaagagt atcaagcact atttacaaga ggaaatggaa aagactggta
      661 aaacgaagat taaaggtgaa ttattcaaca ttggaattca aaataatcca gtatcggtca
      721 atgtaatcaa tgagaactta attccaaaag gattctttac ccctgtgcct cccaaattgg
      781 ataaaaagca attgaaggag gaactgaagc acggagatat tcctggtgct gaactcgtcc
      841 aaacaaaagg tttgaggata agatgatgaa ggaggagaaa taatggaaca cgaaaacgta
      901 aaagacgctt tgaaagctgc tatcgaaatc gcggaagcta aaggaataaa ggttgacggc
      961 aaaccagcaa ctgtccatga cattcaaaat ttgacgaaag aacatcttta ttttatcgcc
     1021 gacttgcttg gtttgtcgga attgtattta gacaaataac cccacacgga aggagtggct
     1081 agggtggttg tattacaagt aatagtctgc gtaacattca tttggtcgtg cataccggtt
     1141 tgtatatata tcatgaagaa gcttcgccct gagtcttaca aattatacgc aatttacagc
     1201 ataacagtag ctcttgcatt tacattgttt gcaatttcta ggcttccgtt ttaatcgtgg
     1261 gaatgaattt ttaatgaact aaattatcta taaagcaaaa aaaggagaaa caacattgaa
     1321 aaacaaacta gcaaaaacaa cagcaatcat cggactagca ctaggtagcg gcgttatcgg
     1381 ctacgcagca agcaatgcgt ttcaagattt ggacacgatc aaagcaaatt tcaacacagt
     1441 cctacaatac ggtcaaacga aatcgcaacg tgtgtcagaa cttgaatcac agctatccaa
     1501 caacactcgc acacaggagc agctgaaagc cgaaattgag caaatcaaat cggacaagca
     1561 gaaggaaatt gaagccaagc aacgagaagt cgaacaaaag caacaggaga tcgctacaaa
     1621 gcaacaggaa gccgatagct tgcgccaaca actatccaca gtgcaaaacg acaaggagca
     1681 gttagaacag cgcgtgagcg agttacggca gtatacggat caaaaggtag gggagttggg
     1741 gaaatgaaag agttgactat ttatgaccca aatatttcat gggctaaaca cacaattaaa
     1801 gtttctttta tgatttgggg ttacaaagga tatgttactt acaaagtggg cggtaacact
     1861 aagggtttat cattaatagc tattgattca gatgatctat atgatgctaa tttcgaggat
     1921 aacccagtta atttccgtga ccttgatgaa gattggttct ctatggaact tactaacgat
     1981 aaaggcgaca gtacacttgt agaagatgaa ttcgacagac taggtgatta tattgtcggt
     2041 gttgaaatta ttgcgcacga accagagtag gagagtgaat acaaaatgat taaaagaatc
     2101 actatatcag ttgaagaaga tagtggatca acaatgcagc ataccgacaa tcgaaaaact
     2161 tttttaacca atagaaagga gaattttgat gcagattaag aaggcgacgg atatcgaaca
     2221 aaccaaggga acatatttga tctacggtgc tccaggaaaa ggtaaaactt caacggctaa
     2281 atattttcca gggaaaacac tgatccttga tattgatcgg acttcaaaag tccttagagg
     2341 tgagaaaaat gtcgatatcg tatatatcga caacgaagat acctggaacg actggggcaa
     2401 cacgttggct gatttaacta ccaattatgt aggtgtttat gacaacgtgg ttgtcgataa
     2461 tgtcagcgaa ttggaacggt gtattctgtc cagtttaggc gcagaaggaa aaaacaacgg
     2521 cgttccttcg caaggcgatt atcaatatat gcaatttaga atggtgaaca gcctacgcta
     2581 tatgaaaaat ttagacagca accttatctg gaccgcatgg gaagaaatcg atttgtggac
     2641 ggattcggat ggatcttctt acaacatcgc tttgccgcag atcaatcgaa agattcgaaa
     2701 caatattttg ggattatgtg acgttgttgg tcgattgatg gtaaaggaag acgatgaacg
     2761 cggatttatt ttacaagcca cagattcaac ctatgccaaa aatcaattgg ataatagatc
     2821 tggctgtaag caaagtgagc tgattttaga tggaattgta cgactaccag aatgatttag
     2881 tcgatcgtgc aagacaagcc tatgttgatg gttacaaggc accatgtatc gtatcaccgt
     2941 gcggttctgg aaaatcagtc atggttgcag agatcgctaa acgaaccaca cgaaaaggca
     3001 acagagtgtt gttcttggtc catcgtcgtg aattgattga tcagatcaaa gatacgtttc
     3061 aaaagatggg cgtagataat cagcttgtaa atttcggcat ggttcaaaca gtcgtacgac
     3121 atcttaaaac catcaagaaa ccacaactga tcatcactga cgaaaaccat cacgggttag
     3181 cagcgtccta cagaaaaatc tatgagtatt attcggacgt tccgcgatta ggttttaccg
     3241 cgacacccat ccggttaaac ggtagtgggt tgggggatgt gaacgacttg ctaattgaag
     3301 gagtatcggc taaatggttg attgagaatc atagattagc tccttatgaa tattatgcac
     3361 ctaaattgat cgatacagcc gaattaaaga aagcttcgac tggagatttt acaaagaaat
     3421 caatggataa ggcagtgaag aatactattt atggcgacgt gttgaaacat tataaaacgt
     3481 tagcagaagg tgaacaggca atagcatact gtcacagtat cgatgcaagt aaacacactg
     3541 ctgaaatatt caacgatgct ggttataaag caacacatat cgatgcaaaa actccaaaag
     3601 atgaacgtgc agatattatc gaagcgttca gaaatcatga aattaaaatc ctatgcaatg
     3661 ttgatttgat cggagaaggg tttgatgttc ctgattgttc gactgtaatt atgttgagac
     3721 ctactcaatc gttatcattg tacattcagc aatcgatgag ggggatgcgt taccgaccag
     3781 ataaaacgtc aatcatcatt gaccatgtgg gtaatgtgaa ccgttttgga ttgcctgata
     3841 tggatcgtga atggagttta gatacaaaaa agaaaacaaa aactgatagt gatttatcta
     3901 tcgttcaatg cgcgttttgt tttggtgctt acgaacggcc aaaaggcgat aatatttgtc
     3961 cgtattgcgg agagttgcag ccgattgaag aacgaaagtc agagttagaa atcgatgaat
     4021 cggctgaatt aatgaaagtc ggagaaacga aaattacttt gaattttgag aacaataaat
     4081 attacaacat gacggaagat gaagcagaaa gtatagagga cttatatgcc atagcaagag
     4141 ctaaaggatt taaacctggt tgggcttata tggctgctaa gaggaagggg tggttgtagg
     4201 aatgggcggt atttttggta aaaaagcaga agatgttcaa ggttcaataa taaatggcgt
     4261 aacaataata ggtgatactg gaaaaagaga tagttcaggc aatcaaattg taatcgctag
     4321 aaataaaaat aacgaagttt ttgaatcttc tctatctaat ataaaaagag gtgttgttaa
     4381 cgggacaggc gaagagagtg gctacgttaa tttaaaaaaa gctaatgaaa aataccatgt
     4441 tgaaggaact tacgttaagg ctttgtctgt cggtggaaca aaaggcgtta attgggataa
     4501 acgaagaaat aaatggaaag cacgattata tttcaaagga gaatatgtct tacaaaagga
     4561 tttcaacacc aaagaagaag caatccaagc acgacttgca gcagaagaaa aatatttcaa
     4621 acctatctta gaaaaatatg aaaataaaaa ggagaatgat taacatgaca ggatttaact
     4681 tagacttttc aaaagcgcaa caagggaacg aaatcaaaga cggtacgtac gaagtggtcg
     4741 taaacaaagc tgtagaaaat gcaactaaat caggcgcaga atttatcgat atcgatttga
     4801 tcgttcgtaa cgatgtggat caaccgttcc aaaataaaca cattttcgca aaaatctgga
     4861 aagccaaagc taccggcaaa tataacgaag gtatgatcat ggcaattgca caagcgctac
     4921 agttggaaga cggaaaatca tataacgggt ttgatgaatt gctggcggac tttgttttga
     4981 aaaccgcctc agtacgcgtg aaaactgaag agtcaaatgg atataaaaac gtcaatgtga
     5041 agtcttggga taaaacgaac acacggggcg taatgaatca ccaattcaaa aatggtgatg
     5101 aaccatcctt cggaccggaa cgttcaagag cgacaacggt tagaaatgac aatcttccat
     5161 tttaattgag gtgtaatcaa tgtatgaaaa aattccaaaa gagcttaaaa atctcaaaca
     5221 atggtgtgtt tataagctag tttgggatga aaagcggcag aagcacacga agatccctta
     5281 taacgccaac aacgggcata aagccaaatc caatgacgag agtacctggt ctgattttca
     5341 gaccgctctc gctgctattg atcactacgg aatgagtgga ctggggttct ttttcaaacc
     5401 accttatttc ggcattgata tcgataatgc tgaaggtgaa gttgagcgat ttaaatcagg
     5461 tgatatagaa gaaaatatta tctatgaatt tatcgaaagc atgagatcct atgctgagta
     5521 ttctcaatca ggtactggca tccacattat tgctcgcgga gaattgcccg gcggaagacg
     5581 caggaaaggt gacgtagaaa tgtaccagaa cggccgattc ttcgttatga ctggaaatgc
     5641 cgcatcaaaa tatttagaaa taactgagcc gaatccaaag gatatcaaac gcctatatga
     5701 tcgctacgtt ggagatgaaa aaatcatcca attcaaagaa gaaaatccgc tgatgaatac
     5761 cgttgattta ccggttgaag aaattatcaa acgtgctgaa tcgtcttccc aaggggcaag
     5821 attcaaagtc tttatgaacg gcggttggga agctttttat tcctcgcaat ccgaagcgga
     5881 tatggcgttc gccaacgact tggctttttg gaccggtaga gactttgaaa aaatggatga
     5941 gattttcaga gcgtctgcaa tgatccgtag caaatatgac caaaaaaggg gatcggcaac
     6001 ttacggagaa tctctactaa ataaggcaat tgctgataca aattccgtct acaaccccaa
     6061 acggaaatct gattttaaga tttttatcaa agaccaggaa cagccaaaag aggaaaaata
     6121 ctattcgtac gacgacaccg gcaacgcgga tcggtttact gatatctacg gaacgttagt
     6181 caaatattcg tacattgata aatcttggta ttactacgat gggaaagttt ggctacagga
     6241 caataccggc gaagttcgga aaatgatcga tacgacagta gacatcatgg gaaaagaacc
     6301 gttaacgatc cctgaaggcg cagacgatga aaccaaagaa gctttgatga aagcgaaaga
     6361 aaaacacgtc aagcgttctc gcagcaatgc cggaaaaaac gctatgatgg acgaattgaa
     6421 acatcgacta tccgtattgc cggaagagtt cgacaaagat aaaacgctgt tcaacacaca
     6481 aagcggctat ctgtctttgc atgacggtct tctacacgag catgagatag acaaaatgtt
     6541 tacacgtgtt tcaaatgtcg agtacacaga atcggtcgat tgtcctatgt gggaagaatt
     6601 tattaatcag atttttgaca atgatcaaga attaatcaga tatatccaga aatgtgtcgg
     6661 atattccctg accggatcta ccagagagca atgtatgttc atcctctacg gtcatgggtc
     6721 aaatggaaag tcggtatttt tggaaattat ttctgaattg atgggaaact atgcgatgac
     6781 tatgcaggca caaacgatca tggtcaagca aagccaatca tcagcaaaca gtgatattgc
     6841 acgattgaaa ggtgcgcggt tggtcacttc gtcagaaccg aatgaaggcg tccgactgga
     6901 cgaaggtctg gtcaaacaat tgactggtgg tgataaagtc acggctcggc acctttatgg
     6961 aaaagaattc gagttcgaac ctgaattcaa gttgtggctg gcaacgaacc acaagcccat
     7021 catccgtggg accgatgacg gcatctggcg acggttaaat ttaattccat tcactgtcca
     7081 aatcccggat cacaaaaagg ataaaaatct gaaatttaaa ttgcagacgg agttgcaagg
     7141 gatccttaaa tgggcaatcg atggttgcct gttgtggcag agagagggct tggagaaacc
     7201 aaagtcggtt gttgcagcga gtcaggatta tcgaaacgaa atggatcaaa taggtacatt
     7261 tatcgaaacg tgttgcgaaa caggtccagg actaaaaata tcaggcggag aattatacaa
     7321 agtttatcgt gaatgggcat cagataatgg cgagcatact ttcactaaca cgaagtttgg
     7381 cagagagatt tcaaagaaat atagcaaaga aaaagctggt ggatttatgg tttataaagg
     7441 aatcacatta aaaccaagaa aatacgataa cgttagagaa ctttttaaat aaatctggag
     7501 ggttctggag ggttgagaac gaaaccctcc aacccttaga aacgttgata tatcagtatt
     7561 tatattatat ttttctttct ttctggaggg ttttaaaaaa ataataaagt attaaagtaa
     7621 aaaataaaaa gtaaattata taaaaaagaa taggtttttg ggtctgaccc tccaaactct
     7681 ccaccaaaat cttcctaaat gctttgatac caacgattaa acagtctgga gggttacatt
     7741 gaaatcagaa catcaaatac aagacgagat cagaattgcc ttatcaaaac atggttgcac
     7801 gatttttcga tccaatgcgg gacaagtgac aacgatcgaa gggcggcaat tttacgggat
     7861 gccaaaaggg ttcccagatc tatgtggaca tcgtaattct gatgggaaag caatttatat
     7921 agaagtaaaa aatgaacgcg gtaagttgcg tgaagatcaa aaaaggtttg ctgaattttt
     7981 aaacggtcaa cctgtactat acggtgttgc acgatcagtg gaagaagcat tgaaaattgt
     8041 ggaggaatca tcatgacaac agaagaagtg attcaaatgc gtattcgaag ccttcagcgt
     8101 gagattgacg atctggaacg gacaaaggca gtgatggtca atgaaacggc gaggaaggca
     8161 atcgatttgc acatagagaa tttaagaagg gaaatccatc gattggagga atgagcgtgg
     8221 ataagaaagc agcaatgaaa cgaatcatcg aactgacaca ttctgagaat tggcaagaag
     8281 acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc aatgtggagt gaaaaaacca
     8341 aacggagaac accgagaaaa attgcaatct ggcatggtga ccgaattcta gtaacaggta
     8401 ctgctgaaca gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta
     8461 aacgcgagaa cgttgattcg aaaggtcgtc aatttaaaca ttgggagaag aaataatgga
     8521 ggaactaatc acaaaagtag agcagtgggc aaaagataag ggattggatc aagctgattc
     8581 cagcaagcaa atgttaaaaa cgatcgaaga gattggggaa gttgccgctt ctctagctag
     8641 aaaagatgaa tatggtttaa gagacggaat tggagacgta gtagtaacct tgattatttt
     8701 agctatgcaa aatgatatgg atttgtacga gtgtctgaac caagcataca acgaaatcaa
     8761 aggacgcaca ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt tggaggacag
     8821 cgatgaataa aaaagtatta attgataaac aagcattgat tgatgaatta atgaaaatac
     8881 ctggtgtggg atctaatagt gacgctttag aaacgattaa acgtttccca tcgtatgaac
     8941 cgcagaagcc agttgtgcct aagtttgtgg cggaactact cgactattat cgtcagtcaa
     9001 cagatgttga tttattagct ttattgataa ctttccatga ttggtattat cgcaaaacta
     9061 aagacggtga gcatgaagaa gcaattgatt ggctagttga tcatcctgaa atttatatgc
     9121 gtgcatggct gtttggctac gagctcgaga aagagccgtt gtattgcgta ataatcaatg
     9181 gtagatatct tgtcaaagta ttcagcaata caaacgttgt catattagtt ccagcagatg
     9241 aattttcaca gtacgttact caaaaatttc aattaactga aaaacaaatc aaagcaattg
     9301 atgaaagata ctggccgttt gctgtgccag tggatgaggt ggtagaagga tgagcgaccc
     9361 atacattact aaattagaaa agttgtccga agattttgag aagaataaaa ataaactaac
     9421 taaagataaa cttatagaac tggttaggtt ggttatcaga ggagctatta caagagaaaa
     9481 ggcgcagcaa aacatagaaa tttttgctcg tagtgtccga gatggattag agaagaaagt
     9541 taaacaaaat gaacaacaga caccgcagaa taacaaaact aagaaaacgg gaactgaatg
     9601 tactaaagac aaagtttgaa aaagaatatg gaatttcagc agaagaaaca tataaagtgg
     9661 caagtcagtg tgttgctgat gcgagcggcg ctattcgtaa gtttggaatt tcgatattaa
     9721 gtgatgatcg taaatgggag gaaaaagaat gaaactaaaa gacggatttt actccagcag
     9781 tcatggtatc ggcggtttaa tgctagatat gccgacaaag aaccctaaaa cacgtgagaa
     9841 accaaaattc aaagtcggtg acatggttcg ctgtgaagca gaaggattca tctatccatt
     9901 ttgtggatat gtagaacatc tctataatca ctcagcaatc attcgtattg aaaacacgat
     9961 ggaatgtgac aagtggttag cgaaaagcaa agagaattta gcagtagcga gattggtgga
    10021 tatggaggtt atataaacag cgaaaaaatg tacttctaag agaaaaattt attttttata
    10081 aaaataggaa agtatcatta tgaaaaagac cacagagata aataaaccta ccacataaaa
    10141 aatgaatatt tcttgcgatt caatataggt atatttaaca atttgtacaa taaaaaatac
    10201 tataggtaaa aaagataaca tgaggagctt atttgttcta gagtgtttga caaagtaaca
    10261 aaatgcgacg ataccaatac aaacgggaat ccaaaataac agcatgctcc acatagtcac
    10321 tcaaccatct cctatacgct ttattatcaa tagataatat catttttcgt aataaagata
    10381 aaaaaataag taaggcttct cattaaaata taaaaaagac agccgaccac tgactgccta
    10441 tataagagta ttgaaataaa aagctgctga tataataaat tccacaagtt tattatatca
    10501 cataaaggag cggtttgact tgatgcaatt gttacgagag gtagatttca aacagacaag
    10561 atgtaatgcg agagatgtgc tgaagaactt tcggcgtttg gagcggatgg caggtcgctc
    10621 tttgatagat attaagtcgc cgattattac ggatatgccg aaggcgccga agcacggcaa
    10681 taaggcagaa gacgcaatca ttcagatgat ggatatagaa gcggagagag acgcgattct
    10741 agcagccttg atggctctta gtctgattag tcgtcagata ctctactata gcttttgtgt
    10801 gccagatagc ttctcaaact acagaattag ccgtgaagtg ggttattcag aaagaagtat
    10861 acaacggatg aagtcggaag ctctaataga gtttgcagaa gcatataaac acggaagaat
    10921 aattgcttat aaataatttg gcggcttttt ggcggaatga tggcggtttt tagctattta
    10981 ccagtgatat tatggtagtg tcgaaagatt agtgataggt ctaagacaaa ataataataa
    11041 aaggaacatc gtt
//
LOCUS       DAZKNM010000087        10908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000087 DAZKNM010000000
VERSION     DAZKNM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.088"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8M9_001782"
     tRNA            12..84
                     /locus_tag="ACG8M9_001782"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8M9_001783"
     tRNA            88..160
                     /locus_tag="ACG8M9_001783"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8M9_001784"
     tRNA            168..249
                     /locus_tag="ACG8M9_001784"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8M9_001785"
     tRNA            267..339
                     /locus_tag="ACG8M9_001785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8M9_001786"
     tRNA            351..422
                     /locus_tag="ACG8M9_001786"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8M9_001787"
     tRNA            439..524
                     /locus_tag="ACG8M9_001787"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8M9_001788"
     tRNA            535..608
                     /locus_tag="ACG8M9_001788"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8M9_001789"
     tRNA            618..691
                     /locus_tag="ACG8M9_001789"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8M9_001790"
     tRNA            711..783
                     /locus_tag="ACG8M9_001790"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8M9_001791"
     tRNA            811..884
                     /locus_tag="ACG8M9_001791"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8M9_001792"
     tRNA            898..971
                     /locus_tag="ACG8M9_001792"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8M9_001793"
     tRNA            995..1084
                     /locus_tag="ACG8M9_001793"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8M9_001794"
     tRNA            1097..1170
                     /locus_tag="ACG8M9_001794"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8M9_001795"
     tRNA            1189..1264
                     /locus_tag="ACG8M9_001795"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8M9_001796"
     tRNA            1266..1336
                     /locus_tag="ACG8M9_001796"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8M9_001797"
     tRNA            1350..1423
                     /locus_tag="ACG8M9_001797"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8M9_001798"
     tRNA            1448..1535
                     /locus_tag="ACG8M9_001798"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8M9_001799"
     tRNA            1557..1628
                     /locus_tag="ACG8M9_001799"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8M9_001800"
     tRNA            1651..1740
                     /locus_tag="ACG8M9_001800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8M9_001801"
     tRNA            1753..1826
                     /locus_tag="ACG8M9_001801"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8M9_001802"
     tRNA            1845..1917
                     /locus_tag="ACG8M9_001802"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8M9_001803"
     tRNA            1921..1993
                     /locus_tag="ACG8M9_001803"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8M9_001804"
     tRNA            1998..2068
                     /locus_tag="ACG8M9_001804"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8M9_001805"
     tRNA            2081..2154
                     /locus_tag="ACG8M9_001805"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8M9_001806"
     tRNA            2166..2237
                     /locus_tag="ACG8M9_001806"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8M9_001807"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8M9_001807"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0378544.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8M9_001808"
     CDS             3826..5298
                     /locus_tag="ACG8M9_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0378545.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8M9_001809"
     CDS             5381..6169
                     /locus_tag="ACG8M9_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0378546.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8M9_001810"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8M9_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0378547.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8M9_001811"
     CDS             7019..8731
                     /locus_tag="ACG8M9_001811"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378548.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACG8M9_001812"
     CDS             8734..10506
                     /locus_tag="ACG8M9_001812"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378549.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10908)
                     /locus_tag="ACG8M9_001813"
     CDS             complement(10588..>10908)
                     /locus_tag="ACG8M9_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0378550.1"
                     /translation="LIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLD
                     KKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLG
                     KIEI"
BASE COUNT         3130 a         1964 c         2506 g         3308 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaa
//
LOCUS       DAZKNM010000088        10670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000088 DAZKNM010000000
VERSION     DAZKNM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.089"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG8M9_001814"
     CDS             140..460
                     /locus_tag="ACG8M9_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378551.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG8M9_001815"
     CDS             912..1112
                     /locus_tag="ACG8M9_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0378552.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG8M9_001816"
     CDS             1259..4048
                     /locus_tag="ACG8M9_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0378553.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG8M9_001817"
     CDS             4095..4622
                     /locus_tag="ACG8M9_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0378554.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG8M9_001818"
     CDS             4643..5833
                     /locus_tag="ACG8M9_001818"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0378555.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8M9_001819"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8M9_001819"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0378556.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG8M9_001820"
     CDS             7966..8640
                     /locus_tag="ACG8M9_001820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0378557.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG8M9_001821"
     CDS             8637..8978
                     /locus_tag="ACG8M9_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378558.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG8M9_001822"
     CDS             9008..9367
                     /locus_tag="ACG8M9_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0378559.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG8M9_001823"
     CDS             9627..9749
                     /locus_tag="ACG8M9_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378560.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..>10670
                     /locus_tag="ACG8M9_001824"
     CDS             9891..>10670
                     /locus_tag="ACG8M9_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0378561.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGE"
BASE COUNT         3566 a         1885 c         2293 g         2926 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa
//
LOCUS       DAZKNM010000089        10545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000089 DAZKNM010000000
VERSION     DAZKNM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.090"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..970
                     /locus_tag="ACG8M9_001825"
     CDS             80..970
                     /locus_tag="ACG8M9_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0378562.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1295..3766
                     /locus_tag="ACG8M9_001826"
     CDS             1295..3766
                     /locus_tag="ACG8M9_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0378563.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3869..5605)
                     /locus_tag="ACG8M9_001827"
     CDS             complement(3869..5605)
                     /locus_tag="ACG8M9_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0378564.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5602..6306)
                     /locus_tag="ACG8M9_001828"
     CDS             complement(5602..6306)
                     /locus_tag="ACG8M9_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0378565.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6437..6940
                     /locus_tag="ACG8M9_001829"
     CDS             6437..6940
                     /locus_tag="ACG8M9_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0378566.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6900..7238
                     /locus_tag="ACG8M9_001830"
     CDS             6900..7238
                     /locus_tag="ACG8M9_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0378567.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7259..8677
                     /gene="ffh"
                     /locus_tag="ACG8M9_001831"
     CDS             7259..8677
                     /gene="ffh"
                     /locus_tag="ACG8M9_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0378568.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8789..9367
                     /locus_tag="ACG8M9_001832"
     CDS             8789..9367
                     /locus_tag="ACG8M9_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0378569.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9371..9892
                     /locus_tag="ACG8M9_001833"
     CDS             9371..9892
                     /locus_tag="ACG8M9_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0378570.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9971..10525)
                     /locus_tag="ACG8M9_001834"
     CDS             complement(9971..10525)
                     /locus_tag="ACG8M9_001834"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0378571.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3449 a         1952 c         2213 g         2931 t
ORIGIN      
        1 ctatattttt acagcaacca tacgagggat ttacagtcga ctgataaact ttagttgtaa
       61 ataagaaagg agcaataaaa tgaaaaaagt attgtttact ctaggtatga tgggaattct
      121 tttagccgga tgcggctcag gatcaggaaa tacagatggc tctgccacac aaaataattc
      181 aagtgactca aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt
      241 agtcgatgca gcacaagaaa gctttgtaca agaaaatcca aattatcaaa tctctgtaca
      301 aggaggagga agcggaactg gtttgagcca agtcgaagct ggagcagtta ctatcggaaa
      361 ctctgatgta tttgcagaag aaagagacgg agttgatgct tcaaaattag tggatcataa
      421 agtagcagtg gtcgggatgg cgccaatcgt caataaagat acagatgtca aagatatcac
      481 caaacaggaa ttgattgata tctttacagg aaaaatcact aactggaagg aagttggcgg
      541 gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt
      601 cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc caagaacaag attcatcagg
      661 aacagttcgg cagctggtaa gtcaaacacc aggagcaatc agctatttag ctttctcata
      721 tctcgatgat tccacacaag cactaagtat cgacggtgta gaaccaaaag aagagaatgt
      781 agcagataac agctggggaa tctggtcata tgaacatatg tacacgaacg gaaaaccttc
      841 ccctgaagtc caaaaattct tagactacat gatgacagaa gaaatccaag aaggacctgt
      901 caaagaattg ggctatttac caatcacaat gatggaagta gaacgtgatc acgaaggaaa
      961 tatcaaataa ctagcagata aacatgccac ggtagtcgtt actattaagg aaaaatcaac
     1021 tatgtttccc acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc
     1081 gttttttgag ctaataccga gggtcagctg attaaacacc gtttattcga aaaaaagagc
     1141 tacgacatag aactgtcgta gctctttttt tgctatatga caggaatgaa aggtgtttgc
     1201 aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag aagtttggaa
     1261 atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga taaatcgaag
     1321 taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt ttccatcgta
     1381 taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa
     1441 ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca tatttacgta
     1501 tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat cctataagaa
     1561 cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc aggaaattcg
     1621 atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca atgtcatacg
     1681 ggacgggata gaacaaacaa tagatataga agaccttgta atagatgaca ttgttatttt
     1741 agagactgga catcaaattc cagcagatgc agaggtcgtg gaaggaaaac tgcaagttaa
     1801 tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg atccattaat
     1861 gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag ttggagcaga
     1921 ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag aacagtcgga
     1981 gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta ttcctatcgg
     2041 tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag aaagtgtcgt
     2101 tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc tgacgacgat
     2161 tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac acaatatgaa
     2221 aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag gaacgatcac
     2281 agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa taatcagtca
     2341 gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag gagacaatgc
     2401 aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct atcaccatgt
     2461 tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt
     2521 tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt
     2581 ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga
     2641 agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg ctaatcctat
     2701 cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag ccatcaaagt
     2761 gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag ggatttcaca
     2821 tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag cagctgtcga
     2881 aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat
     2941 aaagcgtaac aatacagtag ctatgacagg tgatggcgtg aatgatattc tagccatgaa
     3001 agaagcagac tgcagtatag cgatggcttc cggaaatgaa gccgccatgc aggcctcaca
     3061 agtggtctta cttgattctg acttttcacg catgccagaa gttgttgcag aaggacggcg
     3121 cgttgtgaac aatattgagc gatcagccag cttgttttta gtgaagaata ttttctcctt
     3181 tttgctctca ctgttttcag tgatctttgc tctgacctat cctttagaac catcacaaat
     3241 cacattgatt agtttgttta ctatcggact tccatctttc ttgcttgctc tagaagaaaa
     3301 caaaaagcga atcagaggga aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg
     3361 actaacagat atgatcgtag tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa
     3421 taaaacagat gtttctacag catctaccat gcttttgatt gcagtagggt tcctcgttct
     3481 ctataaaatc tgttcaccgc tgaataaatt cagaagtcag atcattcttt tctgtgctag
     3541 cggtaacttt ttttcggtca tctttttgca taagctgttt tcaatcaccg gtatctcagc
     3601 tgtatcactt ttgctgttga gcattctatt tttctcggca gattcgattt ttaggcattt
     3661 gaccacattg gtagaatatt tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag
     3721 tcgcgtagct aaaaaatttc tatcgatatt taaacgagaa aattgacgaa aagtatctca
     3781 aaaaagagta aaaaagaaca accactcgtg tatctttcga agcaggatga gtcatccaac
     3841 tttaaaacgc acgagcagtt gctctttttt acattttcgg aatggtaacg gtgaaagtcg
     3901 aacctgttcc aagatgactg tctacagtaa tcgtaccatt aaggagttgc acgtagtctt
     3961 ttacaatcgc taaccctaat ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc
     4021 ggtaaaaacg ctcaaagatc cgttcctgat cctcaatgtc gatacctata ccaaagtctt
     4081 ggacagaaaa cgatagatca tctgttgcct gatagcggat aataatctcg ctgtcgcttt
     4141 ttgaatattg gacagcattt tcaatcagat ttttgataat tggataaagg atatctgttt
     4201 tagtagtaaa tggttcgttc tttccaatca atcgaatcgt caaccgtttc ttttcgatga
     4261 ttggctgata attttgaacg atttgctgga aataaggttc tgtataaatt gtctggatct
     4321 cgtaagaatt ctcgccatct tttgacagct ggatgatttc ccgaatcagt ttatctaacc
     4381 ggatcgcatc tttttgcatg atctcaagaa aagaagtcag agtttgcggg tcatcttttg
     4441 caccatccag taaagtctcg gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt
     4501 gagagacgtt gccaacgaaa tccttttgta gcttttctaa acgtcgcact ttcgttaagt
     4561 catacgccac ccctagaatc tgtccggtac cttctgaatt agtaaagtag cgcaaactca
     4621 agtctaatgt ctgccgacct tctgtaaggg tgatctcctg atggatcagc ggatgctcag
     4681 gagtcacttg atgaatcagc tgaatgagtt ttggctcttg aatgacttct gtgtaccgct
     4741 gttcaggttg atagcttacc acgtttaaat gtagctgcat ctttggatta agcagtagta
     4801 aacgagaatc agcatcatca atcaggaata cgccaatcat cagttcgttc agtaacgtat
     4861 ataattgctc ttctgtggta gtacacgccc gatacatctt actcatttgt tcgctcaacg
     4921 cattaatcgt ctgatacaat tcttcccatt gatcgggtgt ttgcatgatt acttccgcct
     4981 gattaggatt ttgcaccatt ttttttagta cgggcagcac ggttttcaat ggctggtttt
     5041 tttgatagat cagataatag atcattcctg caagaacaag gaagaaagac agaaagaaga
     5101 aaaagaccca acggcgaaaa ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga
     5161 tacgtaaaat ggcttctaac tggccacttt ttttgactgg taatgcgaca tataaaagat
     5221 cctcattcaa agtagtactt ttgcgcaaag cgctacctag gttcccacca ttcaatacgg
     5281 ctttgatttc gggacgggag tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata
     5341 agatctttcc agtagcatcc attaaagtaa tccgttcgtt cgactgatgg acgaattctt
     5401 ccaaagcagt tttgttttgc gctgcttgga ggttttcgac atctaattgc cggatcagca
     5461 gttgtgcttt ttttgtcagg tagctttctt gctgttccac gatctgctgt tgaaaataat
     5521 gggaaatcaa ttgccagcca ccaaaaaaca aactaactaa aagaataaga aagaagagac
     5581 gttcataaat ttttttccat ttcatcgttt tggttcctga aacttatagc caaatcctct
     5641 aatagtcacc aagtactttg gatgttttgg atctcgctcg attttttctc gcaaatgact
     5701 aacgtgtaca tctacgattc ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag
     5761 caatgtgtct cggtcgatca cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa
     5821 ttctttcggt gttagttcaa tcagatggtc gtctacggtc acttgataat tggtcaaatc
     5881 agcagtgatt tgtccaatcg atagatattc aggctcagct tcatcttttg attgcttacg
     5941 aggttcaatc cgtctgaata ttgctttcat acgggctaat acttcgcgag gactaaatgg
     6001 tttggtcaaa tagtcgtcag cacctatttc taggccaatg atccgatcca cttggtcatc
     6061 tttagccgtc agcatcaaaa tcggtgtttc atttttttct tgacgcagcc gctgagtgat
     6121 ctccatgcca tccatcgttg gaagcatcac atctaaaatg atgaaatcaa atgattgttc
     6181 taatgctaat tctaatcctt cgcctccatc tgtggcactg gttaccttgt agccttcttt
     6241 ttccaaattg aacgtcaaga gcgttacaat agaaggttca tcatcaacaa ctagtatttt
     6301 tttcattcaa atctccttta aataggtttc attctgttta tattttagca tagaatcagt
     6361 acaaagggct attttacaaa agaaataaat ggtataatag gaaggcactt tgctgaatag
     6421 aaaggaagag cagtcaatga ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta
     6481 tgatggacag aaaaaagaag taccagaatt gatggaacta gttgaaactg gagaagctgt
     6541 catggtctgt ccagaagtga tcggcggatt accaattcct agagatcccg ctgaaatcat
     6601 tggtggaaat ggattcgatg tgtggaaaga tcaagcaaaa gtatggacga caagcggcga
     6661 agatgtaacg gaagcgtaca aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa
     6721 acacattgat ctagtcatca tgaaggaaaa aagcccttca tgcggaaccc attcgattta
     6781 tgacggaagt ttttccggaa taaaaaaaaa tggtgccgga gtggctactg cttattttat
     6841 tcagcaaaaa atgactgtgc tctctgatga agaatggctg aaaactagag gtgagcagaa
     6901 tggaaatcga aaagaccaat cgaatgaacg cgctctttga attttattcg accttattga
     6961 cagaaaaaca aatgaactac atggaattat attatgcaga tgatttttca ttaggtgaga
     7021 tcgcggaaga atacgaagta agccgacaag cagtatatga caatatcaag cggacaagca
     7081 agatcttgga ggattatgaa aaaaaactcc atcttttttc taattatatt gttcgagaac
     7141 aggtattgga aaatatgact gcatatatta cagataagta cccggaagat aaaaaactac
     7201 ttgaatatgt acagcaaatt caagcaatcg aagaataaat aaaaggaatg aagaaaatat
     7261 ggcatttgaa agcttaactg aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg
     7321 aaaagtatca gaagctgacg taaaagaaat gatgcgggaa atccgccttg ccttactaga
     7381 agccgatgtt aacttgcaag tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt
     7441 aggggcagaa gtgctggaaa gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga
     7501 agaattgacg atcacacttg gttctgaaac agcagagtta aataaatcac caaaaatacc
     7561 aactgttatc atgatggctg gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt
     7621 agccaattat ttaaagaaaa atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata
     7681 tcgaccagct gcgatcgacc aattgaaagt attaggtcaa cagctagacg ttcctgtatt
     7741 tgatatggga actgaagtca gcccggtaga gatcgtccgt caagggatgg agttagcaaa
     7801 agaaaagaaa aacgactatg tattgatcga tacggcagga cgtctgcata tcgatgaaac
     7861 attgatggat gagttgaaac aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt
     7921 tgtcgatgcc atgactggtc aagatgccgt caacgtagct gatagcttca accagcaatt
     7981 aggtattaca ggggtcgtca tcactaaact tgatggggat actcgaggcg gggcagcact
     8041 atcgatccgc tcagtgacag gcgcaccaat caaattcatc ggttctggtg aaaaattgac
     8101 tgacttagaa gtattccacc cagatcgtat ggctagccgt atccttggta tgggggatat
     8161 gctgacattg attgaaaaag cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc
     8221 acagaaaatg cgggaaaatt cattcgattt caatgacttt attgaacagc ttgatcaagt
     8281 gatgggtatg ggcccactgg aagatctaat caaaatgatc ccaggaatga atcaagtccc
     8341 tggaatcgaa aacgtcaagg tagatccaaa agatgttgaa cgcaaaaaag cgatggttta
     8401 ttctatgacg ccagcagaaa gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg
     8461 aattgctgcc ggttctggaa acagtgttgt tgaagtgaat cgaatgatca aacaattcaa
     8521 ggaatcaaga aaaatgatgc agcaaatgtc caaaggggat atgaacatcc caggtatgga
     8581 ccaaatgttt ggttcaggcg tcaaaggaaa actaggcaaa atggcgatga atcgaatgat
     8641 caagaaaaat aagaagaaaa agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac
     8701 agactgaaaa tgtctactgc cggagctttt ctatcagcaa tttttattct ttactaatac
     8761 aattactcta aacgaagagg tggcacagat gagatgtccg tggagtgaac aaacagaaag
     8821 tatgcggcaa tatcatgata ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt
     8881 ccgaaaattg atgcttgata ttaatcaagc cggattgagc tggcagacga ttttaaataa
     8941 aagtgaagca ttcgatgaag catatgatcg attcgatatc aaaaaagtcg caaattatgg
     9001 agaaacaaaa atcagtgaac taatggaaaa caaaggaatt attcgtaacc gaagaaaaat
     9061 cgaagcagcc gtccaaaacg ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt
     9121 ttcagagtat ctttggtcgt tttctaatgg aaaaatcgtt gattcttctt atcattcaca
     9181 agaagaagtc ccaacaacaa gtcctctttc agatgaatta gcaaaagatc taaagaaaag
     9241 aggatttaaa tttatcggaa gcacaacaat ctatgctttt ttagaagctg tcgggatcat
     9301 caatgatcac cttgacagtt gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg
     9361 cgaataaagc atgcgaaaaa tcatatttta tggtgctata agtttagatg gctatttggc
     9421 aacgaaaacg gatgatcttc aatggctttt tgacactcca acaggtaagc aaactactta
     9481 tgaagctttt tatgaaacaa tcgatacgac catcatgggc agaaaaactt atcaggaagc
     9541 aaaaaaatat ttggagaccg aagcaattta tccagagaaa aagaatttcg ttttttccac
     9601 ccacgcacaa ttacaattgc cagatgctga ggtcgtaaac gaagatcctg ttgcttttct
     9661 aaaaaatcta caaatgacag aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat
     9721 caaaccgcta ttggaacatc aaatgattga tgaatggtat atccagatta cccctgtatt
     9781 attaggggac gggattcctt tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct
     9841 agacacgaca cgattcggtg aatttatcga attgcattat gtacgaaagt agaagtaaat
     9901 aaaataaaaa aggcaactat atgcagaaat gtcctgtaca tagttgcctt ttttattacc
     9961 gttatgcttt ttactctgtt aatttgttag aaaaatctag atacgcatct aaaatcgttt
    10021 ggaagaaact taatgttcct tcaacgatat taccattttc atcaattaga ttcgcaacgt
    10081 ttccgatata tgcttccggt tgctgcaagg ttggcacatt caaaaagaca agtgattggc
    10141 gtagatggtg gtttgcgcca aagccgccga tcccacctgg tgatgcagta acgactaaac
    10201 ctggtttatg atcccaaaca ctgtgtccat aaggtcttga gcctacatct aaagcatttt
    10261 tcaatactgc tggcacagaa cggttgtatt caggagtaac gaaaacgaca ccgtctagtc
    10321 ccctcacttc ctcacgaaaa cgagtccatt cagcaggagc ctgttctgga gtttcgagat
    10381 cctcattgta aaatggcaga tcatcgattt ttacaaatac actttcgtac ccttcaggaa
    10441 gcaatttcgc aaaagcttct gctacttttt tgttgtaaga atcttttctt aaactaccaa
    10501 taaaaaatcc aatttttttt gtcatacgat ttttcctcct tcaaa
//
LOCUS       DAZKNM010000090        10518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000090 DAZKNM010000000
VERSION     DAZKNM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.091"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG8M9_001835"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG8M9_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG8M9_001836"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG8M9_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG8M9_001837"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG8M9_001837"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0378572.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8M9_001838"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8M9_001838"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0378573.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG8M9_001839"
     CDS             5704..6489
                     /locus_tag="ACG8M9_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0378574.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG8M9_001840"
     CDS             6502..7353
                     /locus_tag="ACG8M9_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0378575.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG8M9_001841"
     CDS             complement(7477..7929)
                     /locus_tag="ACG8M9_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378576.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG8M9_001842"
     CDS             complement(8394..8762)
                     /locus_tag="ACG8M9_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0378577.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG8M9_001843"
     CDS             8912..9394
                     /locus_tag="ACG8M9_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0378578.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG8M9_001844"
     CDS             9431..10441
                     /locus_tag="ACG8M9_001844"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0378579.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3577 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaa
//
LOCUS       DAZKNM010000091        10424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000091 DAZKNM010000000
VERSION     DAZKNM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.092"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..271
                     /locus_tag="ACG8M9_001845"
     CDS             <1..271
                     /locus_tag="ACG8M9_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378580.1"
                     /translation="WMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVDENP
                     TNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            290..1498
                     /locus_tag="ACG8M9_001846"
     CDS             290..1498
                     /locus_tag="ACG8M9_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0378581.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1646..2011)
                     /locus_tag="ACG8M9_001847"
     CDS             complement(1646..2011)
                     /locus_tag="ACG8M9_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0378582.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2158..2931)
                     /locus_tag="ACG8M9_001848"
     CDS             complement(2158..2931)
                     /locus_tag="ACG8M9_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0378583.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3105..3965)
                     /locus_tag="ACG8M9_001849"
     CDS             complement(3105..3965)
                     /locus_tag="ACG8M9_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0378584.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3965..5383)
                     /locus_tag="ACG8M9_001850"
     CDS             complement(3965..5383)
                     /locus_tag="ACG8M9_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0378585.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5440..5889)
                     /locus_tag="ACG8M9_001851"
     CDS             complement(5440..5889)
                     /locus_tag="ACG8M9_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0378586.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6334..6597)
                     /locus_tag="ACG8M9_001852"
     CDS             complement(6334..6597)
                     /locus_tag="ACG8M9_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0378587.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6696..7991)
                     /gene="rho"
                     /locus_tag="ACG8M9_001853"
     CDS             complement(6696..7991)
                     /gene="rho"
                     /locus_tag="ACG8M9_001853"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0378588.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7988..9259)
                     /locus_tag="ACG8M9_001854"
     CDS             complement(7988..9259)
                     /locus_tag="ACG8M9_001854"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0378589.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9424..10293)
                     /locus_tag="ACG8M9_001855"
     CDS             complement(9424..10293)
                     /locus_tag="ACG8M9_001855"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0378590.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         2899 a         2216 c         1834 g         3475 t
ORIGIN      
        1 ctggatgaac acaatcatta atcgattaga aacagcatat caaacaagat ttgatatgaa
       61 agcttcttta gtctttttaa atgatgcgta tcaaaactcg atcgaattga tcaaagcagt
      121 ggatgagaat ccaacaaacg aatgcgaaga atttttaaat ctgtttatgt ctacaagaga
      181 tttatttatt cgccaacttg ttgatcgcta tccatccaat tatcacgatg tagaagttca
      241 aatacaaaaa ttaaaagcat attcagctta aggggaatgt ataacataca tggaaaagaa
      301 attatttaat aaatatttta ttggaatcac attgatcaat tttattgtct acctgattta
      361 ttatctgttg atggtcatca tggcagtcat tgcgcaagac accttacacg ccagcttgag
      421 ccaagcaggt tttgcttctg ggatctatat catcggaaca ttgctggcac gcttattcat
      481 ggggaaacag ttagaactat ttggtcggaa gtttacctta agaggcggcg cgatcttcta
      541 tctcctttca acattagcat atctttatac gccaactatc ggggcattgt atttagttcg
      601 attcttgaat ggattcggct acggtacagt ttctacagct acaaatgcta tcgttactgc
      661 atatatccct gcatctaaaa aaggggaagg catcaattat tatggattaa gtactagttt
      721 agctgccgct gttgggccat ttttaggaat gattttgctg aatatgactg attttcgttt
      781 cattatctgg ttttcaattg tactggtctt ctttgttacg attgcctgcc ttgtctttcc
      841 tgtaaaaaat attccattat ctgaagaaca aaaaatatct cttcgctcat ggaatatcaa
      901 tagttttatt gaaaagaaag cacttttcat tacaggaatt gcctttttga tgggtctttc
      961 ttattcaagt gttttatcat ttttatcttc ttatacaaaa gtaatccact tagttgctgc
     1021 tgggtcattt ttcttcgtcg tatatgcttt ggtcatcaca tttacacgtc cattgactgg
     1081 aaggattttc gatgtcaaag gagaacaata cgtgatgtat cctagctatt tattcttaac
     1141 agcaggatta tttttactga gtgtaacgac gaatagtgtg acattgctta tctctggagc
     1201 attagtcggc ctaggttatg gtacgtttat gtcaaatgga caggctgttt gtttaaaaat
     1261 cgtccgcgaa catcgaatca gtattgcttt atctacctac tttattggtt tagacctagg
     1321 cttaggcgtt ggtccttatt tgatggggat gctaaaatct cttacttcat ttagaggatt
     1381 gtatgtgatt gccggagtca ttccattgat atgtacagca ctctatcttg ttaatggccg
     1441 aaaacatagt catgaagcag agggcgaatt gattgaatca ttgaaaaatg aaatttgatc
     1501 ttgatgaata gataaaaaaa accgttcaga tctgaaaata atctgagcgg ttttttgagt
     1561 aaaaatagtt tatgattatt cttaattaaa catctattta tttttaaagc tttcatttat
     1621 cgataagaat agtttaactg ttgatttatt ccattttccg ttcatctggt acgatgcctt
     1681 gacttagcaa ccacttgtgg aagaaccatt ctgccacacc gatcaataga gcaggaatca
     1741 atactttcat ccaaatatcg actgtatcat tcataaaata tagatagagc cacaagataa
     1801 ctgcagctga accagcatct gcgatcgtcg ctgcgatgtt ccctacttta cgtaagatca
     1861 acagatcccc tagcacatag atcatgacta caataattac cccaacaatc gttgaatcca
     1921 taaagctaac accgtataaa cctgtcagca cgacccacag aatcgcccag atcatgattg
     1981 ccttcactac caacgccttc acatgtttca ttttctgcac tcctttctcc tggataaaaa
     2041 agtaagatta tctaacaaga taaccttttc attattatac catcggctga gagaagcgag
     2101 caaagataag gtcctatcta aaaatgagga agccacttag aacattcacg taataattta
     2161 tccgtacaga cattcgatat ggtgttcctt tactttcgca tactgttctt ttgtcagttc
     2221 tttacttgtc accagataat cgatttcatg aaaagcaatg gcttgatgca tgaatttatg
     2281 tgcaattttt tgctgtgtaa ctaaaacggc aagccggtct gattgttttc taaaagcttt
     2341 tttgaccatt gcctctcctt catgagtttc tgttactcct tggctgaaat ctagcccgcg
     2401 acaactcatt atcgctaaat ccgcataata tgacaatgca tcgttatagg cttttgaacc
     2461 gttgattgtt gctcgtttcg gaacaattgc ccctcccaat acagatactt ttgccgttgt
     2521 atattcactc aacatagcag ctgtgacgat gccattcgta atgatctgca aaccatcaaa
     2581 tcttgctagg tttctgacta agtgtaaaga ggttgtactg gaatccaaga ataagaccat
     2641 atcatctcga atcaaagaag aagctgtatc tgcaatctta cgtttttctg tttcttcttc
     2701 taatttggtg atcttcatca ggtaaggcac gtcaattttt cgattgtcaa taagcatggc
     2761 gccaccgcga actctacgga tcaatccact tttttccaga aaaacaaggt cacgtttaat
     2821 cgtggcttcg ctataattta ctaactcgat caaatcggtg actcttgcaa attggttgtt
     2881 ttttagataa ttcacaatct cctgctgtct attctccact tcattcaaca taaaatcacc
     2941 ccatttactt tatatagtaa caggaaaaaa gaggttgtga cagaagtgat tggcttcgag
     3001 aaataagaag aaatttttcg aaaaaactac ttctgaacat cgtgtgtggt taagagggta
     3061 tgacaagact attgtcatac cctcttgacc acacattttt atttttagta taaactagcc
     3121 ttgcctactg tgttcaaaat ttccatttta tgtttgacca cttcttgtac ctttttatta
     3181 gctgctggat aaattgcatt cggctcgtac atcgatggat tttcactcaa tatttctctc
     3241 aaagcattga aaaagacgct tttaagatct gagctcaaat tcactttggc aactccgtat
     3301 tttactgctt cgctgacttc tgcgtccgga ttacctgaac cgccatgtaa aacaaatgga
     3361 agatcgattt gttggttcaa ttctttcagt aagtcgatat ttaattttgg agttctgttt
     3421 ttcggataca agccatgcgc tgttccaatt gctacagcaa gtgtatcgat tcctgtttct
     3481 ttcacaaaaa tcttcgcttg atcaggatct gtataaatga ttgtttcagc acctccttca
     3541 gcagaaccat tattcccaat cgttccaagc tcagcctcca ctgagacatt taccgcatga
     3601 gctaacgcaa ccactttttt cgtaatctgt acattgtcgt cgaatgattt caaagacgca
     3661 tcgatcatca cggaagaata gccgtttcga atcgcccgta agacatcttc tatcgtaccg
     3721 ccatgatcca agtggataac gatcggcagt ttacttgtat aagcataggc tcttactgcc
     3781 gcaacaaact catcacttaa ataggcgatt tcatcaggat gaatttctaa gattactggt
     3841 gcttctaatc tctctgcttc ttcgatgatt gctttcatca tatcatagct gcaaatattg
     3901 aaggctggta ctgcaaattg attctcttta gccactttca ataactcttt cattgtatat
     3961 aacattacgc ttccacctca tttgttagat tttcattttt cgtccacttg tcttgttttt
     4021 tctctttcac tgtttttttc aatagtccaa gcataaaggc tgtagtgact gtacctgatg
     4081 caattgccaa gacatacata ggtaaattgt tcataacatt tggaattagc atcacccaaa
     4141 tccctccatg aggtgccatt gaagtaactc ctactgccat ggaaatccct gcagcgactg
     4201 ctgaacctgt catcaaacta ggtatcacgc ggatcgggtc agataccgca aacggtatag
     4261 caccttctgt aatatacgaa gcgcctaaaa tccagcatga ttgacctgaa gtccgttctt
     4321 ctttggtaaa tttatgtttg aataataaag tggctaaagc aatacctagg ggaggtgtca
     4381 ttcctgcagc catacaagca gcaatcggtg catttactcc agctgacatc aaaccgatcg
     4441 agaaagtcga agtcgattta ttgatcggcc cacccatatc agctgccatc atgatcccga
     4501 taagtaatcc gaacaatagc ccggatgtat ttcccaagct attcagccaa tttgtcaaag
     4561 catccaacac ccatgtgatc ggttgttcaa tcacaaatac catcaaaagc ccaataatta
     4621 agatcgataa aaaggggacg acaatcagct gataaatagc agaaaattct ttttttacgc
     4681 taattttatt tgccagaaaa tcacagatat accctgctag cacgccacca atcattccgc
     4741 caagaaaacc tgaaccacct gtagaggcca gtaatccggc aacaattcct ggggcaaaac
     4801 ctgcttttcc agcaatcgat gtagcaatcc ccgcgcctaa cgcaggaacc atcatggcaa
     4861 acgctgtatc tcctccgatc cttgaaaatg cagctgctaa cacattatag tctgctgagt
     4921 tgggatcagc cgctgtaatc ccaaaggcaa aactcagtgc gatgatgatt ccgcctgcga
     4981 tcacaaatgg aagcatgtaa ttcacaccat tcatgaaatg attatagatt gtcggctttt
     5041 tctcatttgc agctggttct ttttcattag acacaacgaa tacgccaata tctctcaatg
     5101 cttcgtctat tactacttgc ggttctttga ttgctcggct tgtagagaca cgctttactt
     5161 ttttcccggc aaatcggtcc aattcgattt ctttatctac tgcaagaatg attgcatctg
     5221 cctctttgat ttcttgttca gataactgat tttctgtttt tacaccttgt gtttcgaatt
     5281 ttgcttgatg gtttagattt cttgctgtta cttctaattt ttctgccgac atataagtat
     5341 gagcaattcc agtcgggcaa gcagttacac caacgatttt catgattatt ttcctccttt
     5401 ttcttcacta tcgatactcg gacaaagact ctactatttt cattcttgac taatctgtct
     5461 gatgatatct gctggcgtag tggcttcttt tattcccttt ctaaaatctg cattcatcaa
     5521 tcgtctggag agtgtgctga gaatctctag atgcatatct gctgattttt caggaactac
     5581 gatggcaaaa atatattcaa ttggcatttc atctatcccg ctatagtccg ttaatttggc
     5641 tgtatgaaaa aaacacagtg ctggttttgt cacatatttg ctttttgcat ggggaatccc
     5701 aaatccatct tctattcctg tggagatctc ctcctctcgt ttgaatagtg ccttaacgac
     5761 tttttgaaca tttcttacct tatttgtttt tttcaacaca tcccctattc gctcaaataa
     5821 tgatcctttt gaatctatcg atggatcgaa aacaatcagt tcttctgcga tcaattgatc
     5881 aaacctcata aaaaacctct cccccacaaa aaatgttaac gttttcttgg aactgaggtc
     5941 agtatagcat cgctagctgt accgttcaat attatgaatg aacagaggaa aaagacccat
     6001 catccgcgtg tataagtcat taaaatgaac gatctagctc acaaacaatc gcaagtctat
     6061 aacaaaacaa aaagccttcc ttgaaaggaa agcttagctt ctaattttag gtaaatggtt
     6121 ttttacttcg aaataacccc tcttcatttc gttaatataa ggtgttttta tcaagtggat
     6181 aattaaaact atatactagt ttttcattgt tttgtgcatt atgatcctac gactttttca
     6241 gcatcaaacg aagttttaaa ttggcaaaaa acataaaaaa acagactatc cctatcagat
     6301 agtctgctaa acgttgagtt atcagcgttt ttattattcc gcagcgtttg cgtctttgag
     6361 accgtatttt ttgttgaaac ggtccacacg tccgtctgct tgtgtgaatt tttgacgtcc
     6421 agtgtagaat ggatgtgagt cagaagttac ttcgacacgg atgactgggt atgtattacc
     6481 gtcttcccat tcaactgttt cttgtgaagt ttttgttgaa cctgacaaga atttgaaacc
     6541 agttgttgaa tccatgaata ctactgggtg gtaatctgga tggatgtttt ctttcatgat
     6601 tttttcgctc ctttgccctg ctccatttgc tgaaccagag ttgatttgtc gatttacaac
     6661 attaacatct taacatgttt cagtcaaggt tgcaattatc ttttcagatt tctggtcgga
     6721 ttttttttac caaaggagac atcttgaaat tctttgaaaa attgctgatt gttttttgtt
     6781 ttacgcaaaa agcggatcag ttgttccgta tattctaatg aatctccagt catatttttt
     6841 cgaagtttcc aaatttcttc taattcttca cttggcatca ataattcttc ttttcgggta
     6901 cctgattttt tgatatcgat ggctgggaag atccgacgtt cagataattc acgtgaaagt
     6961 tgtaattcta gatttccagt tcctttgaat tcttcataga tcacgtcatc catacggctt
     7021 cctgtatcga ctaaagcggt cgctaaaatc gtcaaactgc caccttcttc gatgtttctt
     7081 gctgccccga aaaatttctt aggtttatac aatgcggctg ggtcaatccc gccactcaat
     7141 gtacgtccgc ttggtggaac gactaagtta taggcccgag caagtctagt aatactatcc
     7201 atcaagataa cgacatctcg tttatcttcg actaaacgca ttgcgcgttc caatactaat
     7261 tcagaaactc gagtatggtt ttgcggctgc agatcaaatg tagaagatac cacgtcgcct
     7321 ttcacgcttc gttctagatc cgttacttct tccggtcgtt catcaatcag tagaacgatt
     7381 aattctactt ccggatagtt ttcggtgata ccatttgcga tttcttttaa tacagaagtt
     7441 ttccctgctt ttggcggtgc gacgatcaat ccacgctgac caaaaccgat tggagaaaag
     7501 atatcgatca ttcgggtaga tagtctttgc ggtgttgttt ccagttgaag ctgtctttgc
     7561 ggataaagag gtgttaaggc tggaaaatgc ggacgttctt ttgcttcttc cggatctttt
     7621 ccattaacgc tttccacgtg catcaaacca taataacgtt ccgattcttt aggtggacgg
     7681 gcttttcctg ccactttgtc cccatttctc aaactgaatc gtctgatttg tgaggcagaa
     7741 atatagatat cttctgcact tggtccataa ttgattggcc gtaaaaaacc gtaaccatct
     7801 tgaccaacga tatccaatat tccttccata taataaaatc cttgtttctc tgcttgtgca
     7861 cgaatcacag ctagagataa ttccttttta ttcatcttac tgtaataagg aattttaaat
     7921 tctttggcat aactataaac ttcttttaac gttttgttct ctagttctgc cattgtcaaa
     7981 taatcgctca ttcagctccc acttcctcta tcatttcaat tgatgcccct aaggctgtta
     8041 gtttctcgat aatatgatca taaccgcgta agatataatc tacattgtaa atcgtcgttg
     8101 tcccttctgc caatagtcca gcaatcacta aacaagcacc tgcccgaaga tcagaagcca
     8161 cgacttctgt gccgtgaagt ttatttggac cattcacgat gatcatattt ccttccacgg
     8221 taatatcagc gcccattcgt gctagttccg gcacatggtt cacacgtttc gcatagatcg
     8281 tatcgatgat ttctgaagtg cctgtagtca tcaatagtaa cgcagtaagt ggctgctgca
     8341 gatctgtggc aaatcctgga taaggatagg tcatgatgtt tgcgggtttt aattctttcg
     8401 aggggtaaac ttcgatttca tcttcgctga ttttcatatg gacaccaatt tcctgcaatt
     8461 ttgctataaa gctttccaaa tgttcaaaga tcacattttt cactttgata ccatttccta
     8521 ctgcagctgc taatgctaga taggttcctg cttcgatgcg gtcaggaatc atgaaatgcc
     8581 gacagccatg taatgtttcg actccttcga tacggatcac atctgtaccc gctcctcgaa
     8641 cttttgcccc catattattc agtaaagttg ccacatctat gatttccggt tctcgtgcag
     8701 cgttttcaat tactgtctgt ccttttgctt ttacagcagc taacatcaca ttgatcgttg
     8761 ctccgactga aaccacgtcc atgaaaatcc gattgccacg caatccttta ttttctgtcc
     8821 gtaaatacat ggcaccatgt tcatttgtca cttcagcacc tagagcttca aatcctttga
     8881 cgtgtaggtc aatcggtcta gggcctaaat aacagccacc tggcaggcct actaccgctt
     8941 cgccaaattt accaagcagt gttcccataa aataataaga agcacgcaaa ctattgattt
     9001 ttccgtgcgg catcggaatc gatacgactt ctcgaggatc gatttctagt atgttgttgg
     9061 aaaaatggac agttgcgccc atgatctcta aaatttcaat taatgaatga acatcttgta
     9121 tgtccggcac accttccaag atcactgggg aatcagctaa gattgcagct ggaatcaatg
     9181 cgactgcact attttttgca ccgctgatcg ttacttcacc agctaaaggt cggtttcctt
     9241 tgattacgat ttttttcatc tgtttccacc ttgtttatta atcttttttc atggatatgc
     9301 ttgtatttta tcatagataa aaaaaagaaa cgagcatggt ctgccgtttt aagtaaaaaa
     9361 gaatggagag cgacttcaaa agtcgttctc cattcctata tatcgtatct gttatgtgga
     9421 tgcttacgct ttgttagctg aaccgaacca gtcgatatga gatttagcat cttcaacgat
     9481 tgctgcttta cctggagcta ataatttacg agggtcgaat cctttacctt ctttatcttt
     9541 accagcttcg atatatttac gagtagctgc agcaaatgat aattggaatt cagtgttaac
     9601 atttactttt gagataccca ttgagatagc tttttcgatt tgatcttgag gaatacctga
     9661 tccgccatgt aatactaaag gcatgtctgg acctacagca tcagcaattg cttgtaagtg
     9721 gtcaaaagct aatcctttcc agttttctgg atattgacca tggatgttac cgataccgca
     9781 agctaagtag tcgatacctg tagcaaccat ttgtttgcat tcttcaatat cagctaattc
     9841 gccgttaccg atgattccgt cttcttctcc gccgattgaa ccaacttcac attcaactga
     9901 gatacctttt gcatgtgctt tttcaacaac gtctttagct aatttcaagt tttcttcaaa
     9961 tggtagatgt gaaccatcga acattactga agtatagcct aattcgatac attccaaagc
    10021 agcttcgtaa tcaccatggt ctaagtggat agcaactgga acagtgatgt tcattgcttc
    10081 aactaaatca gtgattaaat ctttacagat tttgtaacca cccatgtatt tagctgcacc
    10141 cattgatgtt tggataagta ctggtgcttg ttttgcttca gccgcttcaa ggattgcttg
    10201 agtccattcc aagttgtttg tgttgaatcc gccgacagcg taaccgcctt tacgagcagc
    10261 ttttaaaaat tcagctcctg atactactgg cataataaaa ttcctcctaa gttttgtaaa
    10321 actaaatttg ttaagacaat tcttaaccaa cgaccctatt ttaccaaaca tttcggtagt
    10381 tttctactaa aatcaatgaa aatgaaaatt ttcaagaagt tttt
//
LOCUS       DAZKNM010000092        10367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000092 DAZKNM010000000
VERSION     DAZKNM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.093"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8M9_001856"
     CDS             6..305
                     /locus_tag="ACG8M9_001856"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0378591.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8M9_001857"
     CDS             378..1739
                     /locus_tag="ACG8M9_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0378592.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8M9_001858"
     CDS             1761..2021
                     /locus_tag="ACG8M9_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378593.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8M9_001859"
     CDS             2035..2787
                     /locus_tag="ACG8M9_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0378594.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8M9_001860"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8M9_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0378595.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8M9_001861"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8M9_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0378596.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8M9_001862"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8M9_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0378597.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8M9_001863"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8M9_001863"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0378598.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8M9_001864"
     CDS             7161..8558
                     /locus_tag="ACG8M9_001864"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0378599.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8M9_001865"
     CDS             8639..9079
                     /locus_tag="ACG8M9_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0378600.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8M9_001866"
     CDS             9227..9553
                     /locus_tag="ACG8M9_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0378601.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8M9_001867"
     CDS             9554..10303
                     /locus_tag="ACG8M9_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0378602.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1829 c         2149 g         3101 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgctt
//
LOCUS       DAZKNM010000093        10264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000093 DAZKNM010000000
VERSION     DAZKNM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.094"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..1566)
                     /locus_tag="ACG8M9_001868"
     CDS             complement(58..1566)
                     /locus_tag="ACG8M9_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0378603.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1725..1916
                     /locus_tag="ACG8M9_001869"
     CDS             1725..1916
                     /locus_tag="ACG8M9_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378604.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2119..3036
                     /locus_tag="ACG8M9_001870"
     CDS             2119..3036
                     /locus_tag="ACG8M9_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0378605.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3029..4102
                     /locus_tag="ACG8M9_001871"
     CDS             3029..4102
                     /locus_tag="ACG8M9_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378606.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4250..4768
                     /locus_tag="ACG8M9_001872"
     CDS             4250..4768
                     /locus_tag="ACG8M9_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378607.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4749..5141
                     /locus_tag="ACG8M9_001873"
     CDS             4749..5141
                     /locus_tag="ACG8M9_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0378608.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5217..6152
                     /locus_tag="ACG8M9_001874"
     CDS             5217..6152
                     /locus_tag="ACG8M9_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0378609.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6285..7064
                     /locus_tag="ACG8M9_001875"
     CDS             6285..7064
                     /locus_tag="ACG8M9_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0378610.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7057..7374
                     /locus_tag="ACG8M9_001876"
     CDS             7057..7374
                     /locus_tag="ACG8M9_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0378611.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7618..9228
                     /locus_tag="ACG8M9_001877"
     CDS             7618..9228
                     /locus_tag="ACG8M9_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0378612.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9385..10233
                     /locus_tag="ACG8M9_001878"
     CDS             9385..10233
                     /locus_tag="ACG8M9_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0378613.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3228 a         1800 c         2071 g         3165 t
ORIGIN      
        1 acgaaactat cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta
       61 ttggtaatat agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt
      121 tttcaatgtt tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc
      181 aagttcagct agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga
      241 taaagcgatc aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga
      301 tttcaatcct tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat
      361 atgggctaaa tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact
      421 tgtacgcccg gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc
      481 ttttgtagga ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt
      541 tcggtcttca ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc
      601 ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc
      661 ttgtttagag gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac
      721 acccatgtca gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg
      781 tttgattaca ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc
      841 atcaaagtgg aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata
      901 agcaatgttc agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga
      961 gtaaccagct gcatgtcctt tttggctttc atgatacagt tggttcaagc aagtagcgag
     1021 ctttccactt cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata
     1081 ctcatttttt ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc
     1141 gatttctgag tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt
     1201 cgtggcaggc tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc
     1261 acgtaattca tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg
     1321 gatcttatta cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt
     1381 caatttttgc agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc
     1441 atgcttgtcg tcaactaact tcccaccaaa ttctaaatat aatttgtcat agtggttcac
     1501 tcgttccaaa atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt
     1561 tttcactagg ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca
     1621 actggaagag cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga
     1681 tagttattca ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga
     1741 aaaagaaaaa cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc
     1801 ttgaagatcc cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa
     1861 ctcgtgatga gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa
     1921 taagataaag gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa
     1981 aaaaaggctg taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt
     2041 ataacaattt tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag
     2101 taagaggagg caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa
     2161 ctagaggaga cgaacgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc
     2221 aaagaaaaag caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa
     2281 gctgtccgcg aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa
     2341 gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc
     2401 catgatcgtt ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg
     2461 atgagctatt acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca
     2521 agtctgccag aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa
     2581 ccggagtcaa cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct
     2641 cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat
     2701 ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta
     2761 gagaagaaga agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg
     2821 cctgaaacaa tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt
     2881 cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag
     2941 ggattcggtc aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat
     3001 aatcaatcta atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa
     3061 aaatgaagaa caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag
     3121 taatcctctg gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacatgaaaa
     3181 agaaaaagta actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac
     3241 tgaaaatccc gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat
     3301 tgaaacagcg gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga
     3361 ttttccacaa ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac
     3421 gatcggaaag acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga
     3481 tattggtctg gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat
     3541 caattatacg ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga
     3601 tcaacgatac caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat
     3661 caatgtccaa gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt
     3721 ctctgcaggg cagatcacga tattcaatca caaattgaat caattattag cccatgcaag
     3781 aacacttggt tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa
     3841 tcttcagagc gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat
     3901 gaaaaatcca tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga
     3961 taaaggacag tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc
     4021 atctggagac tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc
     4081 attaatggtg agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt
     4141 ctgttccaaa ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt
     4201 actaaatgga cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa
     4261 taaaatagca cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca
     4321 atttattccg aatttccagc caatgactgc tattttttta tttatagtac tttacttaag
     4381 cttaaaagat gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg
     4441 tgttggacca tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc
     4501 aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt
     4561 tctggcgggc atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt
     4621 gcctcaacct tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg
     4681 caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg
     4741 aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt
     4801 tgacaagttg cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa
     4861 catcgcaaaa agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag
     4921 tggtcttcaa aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag
     4981 aagataacgg cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct
     5041 attggatgtt tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga
     5101 atgatggaca atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta
     5161 aagggcgaat attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg
     5221 aaaattttaa aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag
     5281 ttgctatcag caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa
     5341 gcatccacag tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag
     5401 ccaataaagg acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca
     5461 gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt
     5521 ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac
     5581 tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag
     5641 aattgaatac tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa
     5701 tactgattat ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag
     5761 gaacaatatt gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga
     5821 tcggtttctt gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca
     5881 tcggtgaaag cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg
     5941 agatcagtcg gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg
     6001 cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata
     6061 ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag
     6121 ataaatatga cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa
     6181 tgagaaaaat ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac
     6241 aatgagtccg ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa
     6301 gtgcgccatg gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag
     6361 taggattagc ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac
     6421 tgttatcgat tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga
     6481 cgcaagcatc atttgcaacg acattattga tggttttctt tttagaatta cgctatacat
     6541 tattaggagc aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct
     6601 tttctcaatc actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc
     6661 cttcctggaa taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg
     6721 gtagcaatat gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc
     6781 attttgcttt gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac
     6841 tgatctttac aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa
     6901 atactttcgg attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga
     6961 aggcattcaa aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc
     7021 cgctcttcaa cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt
     7081 ttagttgctt gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt
     7141 actcatcgaa aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta
     7201 tttgcggcgt tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg
     7261 aatatcaaaa tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg
     7321 gcgttgtcag ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt
     7381 ttattttatt aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc
     7441 atttgcaaca gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg
     7501 gcttatgatg caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat
     7561 tttttgctat aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg
     7621 aataaaaaat tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt
     7681 ttaggggtag tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg
     7741 cttgctacga cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt
     7801 acagtcggat ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat
     7861 aacgctgctt gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc
     7921 gcctctgctg cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct
     7981 aacgttaata atgggatact agctatcaga agtttatgcc catctttgat tgctatccca
     8041 attcttagtg caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact
     8101 tctttgatta tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg
     8161 cgcgtattga ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc
     8221 ttcggtgggt tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc
     8281 gaacttaaag attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct
     8341 gtctctatca ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt
     8401 caaatgatcg accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca
     8461 gatcagcaac tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg
     8521 atcctgatct ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg
     8581 aagaaaaaag atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta
     8641 ttgattcttc ttccttcatt gactggaatg tctttattag ctggaccact atacacattg
     8701 ttttttggct atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg
     8761 tttttctctt tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt
     8821 gcgatccgat taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta
     8881 ggattagttg gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt
     8941 gttcgcggct atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc
     9001 aattttttct taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt
     9061 ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt
     9121 gtggctgtag gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg
     9181 tttcgaatgt tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg
     9241 ttttttgctg tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt
     9301 tccaatttta gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt
     9361 ctttgaaacg aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat
     9421 ctgccggaac gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg
     9481 gatgggaaaa tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta
     9541 aaaaacagta aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta
     9601 tatgaggaat tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg
     9661 atttctggct ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc
     9721 attccttatg actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta
     9781 gacttaaatg atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga
     9841 gataatacct atgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt
     9901 ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt
     9961 tctgaaggga aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga
    10021 gctgaagaaa ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg
    10081 atacatgcaa ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat
    10141 ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa
    10201 aaagccatcg gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa
    10261 aata
//
LOCUS       DAZKNM010000094        10102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000094 DAZKNM010000000
VERSION     DAZKNM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.095"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..1068
                     /locus_tag="ACG8M9_001879"
     CDS             124..1068
                     /locus_tag="ACG8M9_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0378614.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            1082..2155
                     /locus_tag="ACG8M9_001880"
     CDS             1082..2155
                     /locus_tag="ACG8M9_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0378615.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            2145..4316
                     /locus_tag="ACG8M9_001881"
     CDS             2145..4316
                     /locus_tag="ACG8M9_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0378616.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(4428..6599)
                     /gene="copB"
                     /locus_tag="ACG8M9_001882"
     CDS             complement(4428..6599)
                     /gene="copB"
                     /locus_tag="ACG8M9_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0378617.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(6619..8805)
                     /locus_tag="ACG8M9_001883"
     CDS             complement(6619..8805)
                     /locus_tag="ACG8M9_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0378618.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(8805..9014)
                     /gene="copZ"
                     /locus_tag="ACG8M9_001884"
     CDS             complement(8805..9014)
                     /gene="copZ"
                     /locus_tag="ACG8M9_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0378619.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9027..9467)
                     /locus_tag="ACG8M9_001885"
     CDS             complement(9027..9467)
                     /locus_tag="ACG8M9_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0378620.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9541..10039)
                     /locus_tag="ACG8M9_001886"
                     /pseudo
     CDS             complement(9541..10039)
                     /locus_tag="ACG8M9_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         2847 a         2147 c         1809 g         3299 t
ORIGIN      
        1 tcgtattctc atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg
       61 taggatgtat tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg
      121 aaaatggaag aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta
      181 gaaaaagcgg tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca
      241 ggtggtcatg tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca
      301 ctagcaaaag ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct
      361 agtgatattc ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg
      421 ggaccgatct ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact
      481 cagtcagcac ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac
      541 cctctagaca atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca
      601 tatcctttgc cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct
      661 tcttttgatg acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta
      721 aatgaagtat tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt
      781 gtcagtcccg aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct
      841 gccatccagc ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct
      901 tatgctttaa tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct
      961 tacgtgtttt cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa
     1021 caaatcattc atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat
     1081 aatggaaaaa atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat
     1141 tgtgcttttt gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac
     1201 tctttatgta tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac
     1261 ttgtacattg cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc
     1321 aaaaaagaga actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt
     1381 tgcagaaaaa aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct
     1441 ttggcagcca aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga
     1501 tagtctagga cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct
     1561 gcctgctttt tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca
     1621 gctgaacgcg taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga
     1681 ttctttcaac cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga
     1741 atacgattat caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc
     1801 aaaatttgaa cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca
     1861 gccggaactt ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta
     1921 ttttgcttct ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc
     1981 aaaaaaacaa atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga
     2041 aacattgtca aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt
     2101 ccaagaaaaa gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg
     2161 aaaaatggct gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg
     2221 tagtagctaa tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc
     2281 ttattgtctg ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga
     2341 taggttgttt gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt
     2401 ttttttcgtc agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc
     2461 cagaaaagac agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg
     2521 tggattacga ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta
     2581 ccgtttttcg ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg
     2641 tgtatctggc agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc
     2701 agtcattgac tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat
     2761 atcttccgaa agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta
     2821 atgatgaagg gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag
     2881 gcttcagtga gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt
     2941 ttacagcgaa ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg
     3001 gttctggatg gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg
     3061 acatcacgag ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa
     3121 aacaagcttt cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta
     3181 gtggacaaac gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat
     3241 cgacacgttc tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa
     3301 gcagtcagtt aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa
     3361 gtttgcctaa acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa
     3421 ttcgtaaagt cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca
     3481 aaacggatac cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt
     3541 caaaagtcgg ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg
     3601 gtatcccagc tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc
     3661 aagggaatga ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact
     3721 ttcctggatt tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt
     3781 cacaaccagt tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa
     3841 gcgaaaatat ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa
     3901 caacactcac accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct
     3961 tgttgttctt tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc
     4021 ttcatttttt tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa
     4081 cattggtacc gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag
     4141 aattcaagct agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg
     4201 gacgacaagc agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa
     4261 taagaaaaac ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata
     4321 aaatttctgg agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt
     4381 cgaaaaaaag gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc
     4441 aatgtcaaag catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact
     4501 gctggactta atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga
     4561 atattgtaac ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct
     4621 agatcaagaa aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa
     4681 tcgattgcga tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt
     4741 ggcgcatcat tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc
     4801 tggacaacag cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa
     4861 taatctgcta ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt
     4921 tttctttctt tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa
     4981 gcaacgattg cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct
     5041 tggtaaggtt tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca
     5101 ttaactattt tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt
     5161 tttagatttt cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca
     5221 acagccaatg gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt
     5281 ttattgaacg catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt
     5341 gttcctgttt tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg
     5401 tttttcaaaa gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg
     5461 atagctaaac ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat
     5521 gcattaggaa gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat
     5581 gcgacataaa acagccattt tgccactttg tcagataatg actctagctt ggatttttct
     5641 ccttgtgctt ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca
     5701 gttacttcga tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg
     5761 gcttgttttt tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca
     5821 cctttcaaga ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca
     5881 ccttcgttta cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt
     5941 actgattctg gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca
     6001 ttcatttcaa tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag
     6061 aaatccatga cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa
     6121 atatatgcaa ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc
     6181 aattccatct tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct
     6241 aagatcaata cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc
     6301 atcattggtg aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt
     6361 ttgaaatttc ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc
     6421 gaatggtcca tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa
     6481 tggtccatct ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt
     6541 tctttgttta ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt
     6601 tttactccat cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa
     6661 tacactgacg gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag
     6721 tgcggcaaaa ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat
     6781 ttttctcaat gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact
     6841 catcagcgtc acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc
     6901 aacatcagct aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt
     6961 tttgccatct ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc
     7021 aaagatatgc tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt
     7081 gtctcctgtc agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt
     7141 ggcttctagt ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc
     7201 taaaaacatg actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc
     7261 aaatgatagg ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt
     7321 aatcgtaccg ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc
     7381 tgcttcttgc agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg
     7441 ttctaaagta taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg
     7501 ctttccttct gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc
     7561 ttctaatgct tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac
     7621 cataatagct gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga
     7681 gacactatgg atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt
     7741 gataaacgcg atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt
     7801 ttggattggg gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac
     7861 tgtctgcttg ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc
     7921 gccaaaaagc gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact
     7981 ttcatctatc gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg
     8041 acggatgact agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc
     8101 gtttcttatc acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc
     8161 tgttttcgtt tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat
     8221 actgctgctt tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct
     8281 caatgcaaag gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga
     8341 cttgattgca tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc
     8401 agacagcact aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc
     8461 taacatcata gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag
     8521 atcgaatagc atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc
     8581 ttcatcgtac aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt
     8641 ggtcgtccct tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac
     8701 cacaccaggt tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat
     8761 acctgtaatg acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct
     8821 ttataaccca atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca
     8881 aacttgaccg taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt
     8941 gcgcttactg tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa
     9001 aattgttgtt tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg
     9061 atacagttac attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca
     9121 ttgatatctt cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc
     9181 ctttttgcac agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc
     9241 gagacagctg gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt
     9301 tttaccaaac gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct
     9361 aagatctgcg tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc
     9421 tcccactctg aatcactaat ctttactggc gttttgactt tttccattta tttcacacct
     9481 ttcgtttaca aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg
     9541 tcaattattt ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt
     9601 tttaaggatg atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg
     9661 ttcattgttt ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag
     9721 tttctccgaa aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag
     9781 agaacgccaa actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt
     9841 atctgcttct ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga
     9901 cggattagat accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg
     9961 gaaagccggt gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc
    10021 caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac
    10081 cacttataac gccgcaaaaa aa
//
LOCUS       DAZKNM010000095         9817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000095 DAZKNM010000000
VERSION     DAZKNM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.096"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..2099)
                     /gene="tkt"
                     /locus_tag="ACG8M9_001887"
     CDS             complement(102..2099)
                     /gene="tkt"
                     /locus_tag="ACG8M9_001887"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0378621.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2201..2452)
                     /locus_tag="ACG8M9_001888"
     CDS             complement(2201..2452)
                     /locus_tag="ACG8M9_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0378622.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2599..3222
                     /gene="lexA"
                     /locus_tag="ACG8M9_001889"
     CDS             2599..3222
                     /gene="lexA"
                     /locus_tag="ACG8M9_001889"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0378623.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3402..3620
                     /locus_tag="ACG8M9_001890"
     CDS             3402..3620
                     /locus_tag="ACG8M9_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378624.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3909..4421)
                     /locus_tag="ACG8M9_001891"
     CDS             complement(3909..4421)
                     /locus_tag="ACG8M9_001891"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0378625.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4435..6732)
                     /gene="recJ"
                     /locus_tag="ACG8M9_001892"
     CDS             complement(4435..6732)
                     /gene="recJ"
                     /locus_tag="ACG8M9_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0378626.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6874..7317)
                     /locus_tag="ACG8M9_001893"
     CDS             complement(6874..7317)
                     /locus_tag="ACG8M9_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0378627.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7318..8106)
                     /locus_tag="ACG8M9_001894"
     CDS             complement(7318..8106)
                     /locus_tag="ACG8M9_001894"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0378628.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8103..9044)
                     /gene="rnz"
                     /locus_tag="ACG8M9_001895"
     CDS             complement(8103..9044)
                     /gene="rnz"
                     /locus_tag="ACG8M9_001895"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0378629.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9221..9808)
                     /locus_tag="ACG8M9_001896"
     CDS             complement(9221..9808)
                     /locus_tag="ACG8M9_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0378630.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
BASE COUNT         2677 a         2196 c         1738 g         3206 t
ORIGIN      
        1 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
       61 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      121 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      181 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      241 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      301 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      361 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      421 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      481 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      541 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      601 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      661 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      721 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      781 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      841 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      901 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
      961 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1021 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1081 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1141 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1201 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1261 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1321 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1381 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1441 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1501 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1561 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1621 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1681 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1741 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1801 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1861 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1921 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     1981 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2041 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2101 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2161 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2221 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2281 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2341 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2401 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2461 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2521 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2581 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2641 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2701 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2761 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2821 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2881 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     2941 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3001 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3061 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3121 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3181 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3241 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3301 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3361 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3421 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3481 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3541 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3601 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3661 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3721 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3781 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3841 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3901 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     3961 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4021 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4081 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4141 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4201 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4261 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4321 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4381 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4441 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4501 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4561 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4621 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4681 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4741 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4801 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4861 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4921 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     4981 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5041 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5101 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5161 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5221 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5281 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5341 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5401 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5461 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5521 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5581 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5641 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5701 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5761 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5821 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5881 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     5941 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6001 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6061 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6121 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6181 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6241 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6301 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6361 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6421 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6481 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6541 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6601 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6661 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6721 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6781 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6841 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6901 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     6961 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7021 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7081 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7141 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7201 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7261 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7321 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7381 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7441 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7501 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7561 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7621 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7681 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7741 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7801 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7861 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7921 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     7981 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8041 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8101 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8161 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8221 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8281 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8341 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8401 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8461 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8521 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8581 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8641 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8701 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8761 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8821 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8881 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     8941 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9001 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9061 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9121 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9181 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9241 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9301 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9361 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9421 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9481 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9541 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9601 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9661 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9721 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9781 aatttaaaat gattactcgt ttgctcatta aaaacgc
//
LOCUS       DAZKNM010000096         9812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000096 DAZKNM010000000
VERSION     DAZKNM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.097"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG8M9_001897"
     CDS             complement(34..2154)
                     /locus_tag="ACG8M9_001897"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0378631.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG8M9_001898"
     CDS             2384..3562
                     /locus_tag="ACG8M9_001898"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0378632.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8M9_001899"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8M9_001899"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0378633.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8M9_001900"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8M9_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0378634.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG8M9_001901"
     CDS             complement(4915..6168)
                     /locus_tag="ACG8M9_001901"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0378635.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8M9_001902"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8M9_001902"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0378636.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG8M9_001903"
     CDS             7746..9092
                     /locus_tag="ACG8M9_001903"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0378637.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG8M9_001904"
     CDS             complement(9400..9786)
                     /locus_tag="ACG8M9_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0378638.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2893 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta tt
//
LOCUS       DAZKNM010000097         9738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000097 DAZKNM010000000
VERSION     DAZKNM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.098"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8M9_001905"
     CDS             278..787
                     /locus_tag="ACG8M9_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0378639.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8M9_001906"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8M9_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0378640.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8M9_001907"
     CDS             2322..3302
                     /locus_tag="ACG8M9_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0378641.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8M9_001908"
     CDS             3424..4842
                     /locus_tag="ACG8M9_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0378642.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8M9_001909"
     CDS             4913..6382
                     /locus_tag="ACG8M9_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0378643.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8M9_001910"
     CDS             6392..6859
                     /locus_tag="ACG8M9_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0378644.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8M9_001911"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8M9_001911"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0378645.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..>9738
                     /locus_tag="ACG8M9_001912"
     CDS             7849..>9738
                     /locus_tag="ACG8M9_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0378646.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVI"
BASE COUNT         3374 a         1420 c         1944 g         3000 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaata
//
LOCUS       DAZKNM010000098         9736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000098 DAZKNM010000000
VERSION     DAZKNM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.099"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..665)
                     /locus_tag="ACG8M9_001913"
                     /pseudo
     CDS             complement(58..665)
                     /locus_tag="ACG8M9_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1149..1376
                     /locus_tag="ACG8M9_001914"
     CDS             1149..1376
                     /locus_tag="ACG8M9_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0378647.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1392..2557
                     /gene="hemW"
                     /locus_tag="ACG8M9_001915"
                     /pseudo
     CDS             1392..2557
                     /gene="hemW"
                     /locus_tag="ACG8M9_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            2791..3834
                     /gene="hrcA"
                     /locus_tag="ACG8M9_001916"
     CDS             2791..3834
                     /gene="hrcA"
                     /locus_tag="ACG8M9_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0378648.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3928..4491
                     /gene="grpE"
                     /locus_tag="ACG8M9_001917"
     CDS             3928..4491
                     /gene="grpE"
                     /locus_tag="ACG8M9_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0378649.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4542..6371
                     /gene="dnaK"
                     /locus_tag="ACG8M9_001918"
     CDS             4542..6371
                     /gene="dnaK"
                     /locus_tag="ACG8M9_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0378650.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6522..7688
                     /gene="dnaJ"
                     /locus_tag="ACG8M9_001919"
     CDS             6522..7688
                     /gene="dnaJ"
                     /locus_tag="ACG8M9_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0378651.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7770..8399)
                     /locus_tag="ACG8M9_001920"
     CDS             complement(7770..8399)
                     /locus_tag="ACG8M9_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378652.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            8411..8584
                     /locus_tag="ACG8M9_001921"
     CDS             8411..8584
                     /locus_tag="ACG8M9_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378653.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(8812..9018)
                     /locus_tag="ACG8M9_001922"
     CDS             complement(8812..9018)
                     /locus_tag="ACG8M9_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378654.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(9044..9463)
                     /locus_tag="ACG8M9_001923"
     CDS             complement(9044..9463)
                     /locus_tag="ACG8M9_001923"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0378655.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
BASE COUNT         3272 a         1704 c         2016 g         2744 t
ORIGIN      
        1 aaagtgtgac aaataatcat cagtaaaatt acttgtcaca ctattgataa ggagtattta
       61 attgaagaga atgattagga gaaaggatct ccgtactttt tatagtaatc atagaatcca
      121 agtttgtata gacgaatcgt tttttctctt atttcttctg tttcaaaatg gactgcttga
      181 agaatcaagt ctgtgaattc tataggagct gttccattcg cagtaatcaa attacgatct
      241 ttcacagctt gttgttctat aaaatcttct ttattctgat aattttccga ttctaaccaa
      301 ggaaattgcg catttccggt gtgtttgtaa ccttgcaata atccgctctg agctaaataa
      361 tcaactgccc cacaaatagc agctactggt acgtctgttt ttaacaattt atcgattatt
      421 tgatataaat gatgattttt tatgtcccat gaattgccac cgatcataat aaaaaggtca
      481 actttttcag gtatttgttc gattaagtaa tcgatttttg tttttaatcc tccaatggaa
      541 gtgacctctg attggataga agcagttttg ttttgccatt tctcattttg gtttagtgtg
      601 ctagtaatat aagcaccttc ccaatcggca tattcctcta atagaaacgt aatcgcagtt
      661 ttcattttca agctctcctt tactatctat aacaaggaca acagttttaa cctacagaca
      721 ttcattaaaa ttgggtagcg acagcgaaaa caacaattct taagttttag acagagtata
      781 cagatagtca acttaattaa atacgaacta aataaaaatg acaggtaatc atttttgttt
      841 accagcatta taacattttg catacatgga tgacaaaaga accaattccc acttgcttga
      901 gtacaatatt aaaagaaact ggaaggcaaa aagtaattat agaaacgaaa aataaatcat
      961 atcgggcact cagacatgaa aacaacccta gagatatata actaggtaac cagtttaatg
     1021 aaagaaaaaa tcatccaaga agtcgattct tggatttttt agtggatttg ttcgccaaat
     1081 acgtttagta tgcgtatatg agaggttgta agcaaagcgt tgtggtctga gcacttaagg
     1141 aggaatctgt gaaaatagct tttcatattt ttgcagattt tgacttaatg ccgaaggtca
     1201 gctttgcgat taccgtttat cgagaggctg taaaagcacc gtgtagctcc gatgacaata
     1261 agcaagcatt tttagagcta ggtttccttg attctaaata tgcggcttat ttcaatgagt
     1321 tggtgcttga acgcgatttt aagaaggtcg aaaacaggca cgacttttcg acgtaaggag
     1381 aaagaatttc aatgatagaa acaataaatc ccgcagaaaa caaggtttct gcgtacattc
     1441 atatcccatt ttgcgagcat atctgctatt actgtgattt taataaagtt tttttagaag
     1501 ggcagccagt agatgagtat gtggaaatgc tgctgaatga aatgaaaata atgctagaaa
     1561 gtcatccgac agcgaattta gaaacacttt acgtaggggg cgggacacca acttctttat
     1621 ctgctaaaca acttgatcga ctgttatcag gtgctagaga gatcctgcct tttcaaaatg
     1681 gtaatgagtt tactgtagaa gcgaatccag gtgatttgac ccgtgaaaaa cttcaagtaa
     1741 tgaaaaacta tggcgtcaat cgtttatcca tgggagtgca gacatttgat aatcgcttat
     1801 tgaaaaaaat tggacgtaaa cacaccgcag aagatgtgta tcaaactatg caatttttag
     1861 aagcagaaaa ttttaccaat gtaagcatcg atttgattta tgcattaccg ggacaaacac
     1921 tagaaggata tctggatact ttggaaaaag ctttggcttt ggatctgcct cactattctt
     1981 tgtactcatt gatcctcgaa aacaaaacaa tgttcatgaa ctgggtacga caaggaagac
     2041 tcgaactgcc tgatcaggaa acggaaacca gaatgtttga agagacgatt gaagcaatgg
     2101 aaaagcatgg acgtcaccaa tatgaaataa gtaactttgg gctctccgga catgaatctc
     2161 aacataacct catgtactgg aacaatgacc actattttgg gtttggagct ggtgctagcg
     2221 gttatcttgg agatattcgt taccgcaaca aaggaccaat ccaacactat ttacgcccat
     2281 tacgagcaaa tgaacgcccg gtattggaag aagaaaaatt gacacgaaaa aatcaaatag
     2341 aagaagaaat gttcttagga ctaagaaaaa aacagggatt actaaaaaac atttctatga
     2401 tcgctatcaa aaaacaattg aatcggtgta cggtgatgtg ctaagggaat tagagcaaga
     2461 agggttactt gttaatgaag aagaccgtgt ttatttgaca ccaagaggga cctttttggg
     2521 gaacaatgtg tttgagcgct ttttgtttga aaaatagttt aaagaatatt tgttttttat
     2581 ctgaatttaa ataaaaaaag ttcaaaaata caataaagga ctattgaaac gttgatataa
     2641 ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga
     2701 gtctttctct tgacaggaga agggcttctt gctatattta taagtggatt agcacttaga
     2761 atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta
     2821 cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattgatg
     2881 tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat
     2941 gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat
     3001 cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg
     3061 attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct
     3121 gagatattat ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac
     3181 cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc
     3241 gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt
     3301 caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg
     3361 acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata tttccaaacg
     3421 acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga agataaggtt
     3481 tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc
     3541 aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg
     3601 gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg
     3661 agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta
     3721 gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa
     3781 ctagcagcta aacttgctga ttactaccgt tcgcttgact tgtcaaactc gtaatgtgaa
     3841 taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg
     3901 actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa
     3961 atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt
     4021 tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg
     4081 gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat
     4141 gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca
     4201 attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg
     4261 aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt
     4321 gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact
     4381 gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac
     4441 aaattacatg atcgcgtgtt aagaccaacc atggtcattg ttgcacaata attgtgcgga
     4501 tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta
     4561 ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca
     4621 tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa
     4681 tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa
     4741 tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac
     4801 cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag
     4861 gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc
     4921 aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac
     4981 caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag
     5041 tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct
     5101 ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa
     5161 tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca
     5221 aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg
     5281 tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt
     5341 tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta
     5401 caaaagtacc agtacgtcaa gcgctaaaag atgcaggttt atcacaatca gaaatcgacg
     5461 aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag
     5521 aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg
     5581 ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac
     5641 cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata
     5701 caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag
     5761 tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa
     5821 gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat
     5881 tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag
     5941 aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg
     6001 taaaagatgc agaagcaaat gcagaagcag ataaagctcg taaagaggaa gttgacctac
     6061 gtaatgatgt agatgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca
     6121 aagtggacga agaagaagtg aaaaaagcag aaacagctcg cgatgaattg aaagccgctg
     6181 ttgaagcaaa caacatcgaa gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc
     6241 aaaacttgac tgttaaacta tacgaacaag cggcacaaca acaagctcaa gaaaacccag
     6301 aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg
     6361 acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt
     6421 tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac
     6481 aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt
     6541 attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc
     6601 gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat
     6661 tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatatg
     6721 accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat
     6781 ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact
     6841 ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt
     6901 tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca
     6961 agtataatcg ggaagaaatc tgtcatactt gtggtgggaa cggtgcaaaa ccaggcacac
     7021 aaccaactac ttgtcataaa tgtcatggtg caggtacgat taatgtagaa cgccaaacac
     7081 cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatgggaca gggaaagaaa
     7141 tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta
     7201 aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag
     7261 aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtcttccgt gtagaagaaa
     7321 gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac
     7381 aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga
     7441 tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt
     7501 tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc
     7561 taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg
     7621 aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc
     7681 ggaagtagtt tttctagtaa aaacgctaag tcattaataa ttaaaaacca gacgaacaat
     7741 catatgttcg cctggttttt ttgtagttgt catgtttgta ccaacaaaaa agccagcttt
     7801 agaatatgat aatcgtcata gtttctaggg gtataaccag tgagagaatg gaacttgttc
     7861 aatcgatatt gcaaggtgtt tttatgaata aataattctt ctgagcaatt tttgatgctt
     7921 ccgttatttt tactgaagca tgccaaagtc ttcttgaaca aatgtacttc ttcttctgtc
     7981 aggcttttca aaatacgttt tacatataat gttcgttgat ctgacgggat tgctggtaaa
     8041 acgatcccga ggtctaaatc ttcaaaaaag cggatctcgt tcacagattg atggaaagct
     8101 atccattcgc aaacttgttt ggcatgaagg aaagattgcg gtgtctcttg ctctgtataa
     8161 caaagcgacc caattccaaa gtttagctgt aactgataca ttttattaaa ctttgtctgg
     8221 attttatcaa gcaatgatag caatgaggct ctggaaatcc gattgactag gataatgatt
     8281 tgttgtctat gtacagtgag caaagcctgt ccgtaataag agaagaccct ttcgagcaac
     8341 tccaataaag gattgatccg ttcttgacac aattgctgat tgtcagaaag ttgtcccaca
     8401 actacataat atggagaaga aaaatcaagc tcaattaagt gagggtggta tcgactatgt
     8461 acttgatatc atcaatgaac cgatggtact ttcttatgcg atggaacagg tagagcgatt
     8521 gcttgtttgt gcaggaaaac aagcgatgcg gctgatacga ttaggtgaga aaataagaag
     8581 ataaaaatac acggtccctt catggcgacc gtgcattttt ggtttttatt aaattttata
     8641 tctaattttc aattatccgc ataaacacta tacattcaag cattttgaag tatagaaaaa
     8701 cactcatctt accacgaatt taccacgatt gaatgagcct tgatatgacc gtaaaactac
     8761 taagggagat aacacgaaac tgtgtatctc ccgatttttt tatacagttg attacttcac
     8821 tccgatttca aatttcaata ttgctttcat catgtctgta tgaatttctc cttttagttc
     8881 ttcatcaacc tgcataatga ttttaccact ttcagagtgg taaaaaggac gcaagcataa
     8941 tttagaaata taaccgtcat aaaacttcaa aatctcttta actgccattt catctccgct
     9001 tgacgctttg caaatcaatt caaaagaggg atagccataa tctttacttc ctttcgctca
     9061 aaagcatttt taacttcctt agtcctctag ttcttctact gcaaatagca gaacgtgata
     9121 catggtacag ttctgaaatt tctctgtcgt tcattccttg aaaataacgt aataaaatcg
     9181 cactacgctg tttttctgat agtctatgta aaactgtacc aagtgtttca tcagatacat
     9241 aaattgtaaa gtgcaagact ttaaatgata cacaatcata aaaacaggta tcaatcgttc
     9301 cacgttcaac ttgtttcgtt tcggctattt cacaaaacaa tacttcacgt ttagaatgtc
     9361 ttgcaagctg tctgtttcga cttttcactt tgttcttaat gacaatcagc attaaagaat
     9421 tgaactgtaa tctgacgata tgctcgaaag aagatgtcct cataatctca ccccctttct
     9481 gtctaggaaa ggaagtgagc cttgctcgtt tatctccttt cactcttagt cccaatgtga
     9541 aagggggatt tgttgcatta ctgataaata aactttgtaa aaaagttctg aatagccaaa
     9601 aaagcatata aacagattta tttctctgtt tacatgcttc tgttattcta tctatatgat
     9661 ttataaaacc acattggtgg acgtacttat ctattgcaga tagacgactt tttttgacaa
     9721 gaacccaatg taagga
//
LOCUS       DAZKNM010000099         9681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000099 DAZKNM010000000
VERSION     DAZKNM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.100"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..510
                     /locus_tag="ACG8M9_001924"
     CDS             31..510
                     /locus_tag="ACG8M9_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0378656.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            543..2771
                     /locus_tag="ACG8M9_001925"
     CDS             543..2771
                     /locus_tag="ACG8M9_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0378657.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            2900..4543
                     /locus_tag="ACG8M9_001926"
     CDS             2900..4543
                     /locus_tag="ACG8M9_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0378658.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4616..5161)
                     /locus_tag="ACG8M9_001927"
     CDS             complement(4616..5161)
                     /locus_tag="ACG8M9_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0378659.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5184..5669)
                     /locus_tag="ACG8M9_001928"
     CDS             complement(5184..5669)
                     /locus_tag="ACG8M9_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0378660.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5659..6471)
                     /locus_tag="ACG8M9_001929"
     CDS             complement(5659..6471)
                     /locus_tag="ACG8M9_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0378661.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6610..8142)
                     /locus_tag="ACG8M9_001930"
     CDS             complement(6610..8142)
                     /locus_tag="ACG8M9_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0378662.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8251..9282)
                     /locus_tag="ACG8M9_001931"
     CDS             complement(8251..9282)
                     /locus_tag="ACG8M9_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0378663.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3223 a         1801 c         1875 g         2782 t
ORIGIN      
        1 ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa
       61 ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat
      121 gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca
      181 ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc
      241 acacgtattt tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct
      301 tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa
      361 gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt
      421 ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta
      481 aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa
      541 gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga
      601 tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa
      661 tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg
      721 ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata
      781 caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg
      841 tagtagattc taataaggaa caagaccaag aattactaga agctatttac caaacagata
      901 cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta
      961 aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt
     1021 acagctatta cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc
     1081 aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac
     1141 caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg
     1201 tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc
     1261 ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga
     1321 tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt
     1381 tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt
     1441 tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag
     1501 ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg
     1561 acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc
     1621 aatctgagca tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca
     1681 ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac
     1741 caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa
     1801 tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag
     1861 atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc
     1921 aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca
     1981 tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag
     2041 taatgccaat agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa
     2101 cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat
     2161 atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg
     2221 tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa
     2281 aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt
     2341 acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat
     2401 ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca
     2461 atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa
     2521 ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca
     2581 aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat
     2641 ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc
     2701 gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc
     2761 gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt
     2821 ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc
     2881 gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta
     2941 tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg
     3001 gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc
     3061 gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga
     3121 agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg
     3181 gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga
     3241 agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt
     3301 agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc
     3361 tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg
     3421 ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg
     3481 gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa
     3541 agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac
     3601 cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt
     3661 cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga
     3721 atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt
     3781 gaaacatgca ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata
     3841 tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt
     3901 ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt
     3961 tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt
     4021 tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga
     4081 agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg
     4141 gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa
     4201 taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta
     4261 taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga
     4321 atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata
     4381 tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga
     4441 tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat
     4501 actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt
     4561 tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat
     4621 aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt
     4681 gtccaagggt tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga
     4741 ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat
     4801 cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa
     4861 cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt
     4921 tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta
     4981 acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa
     5041 cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag
     5101 ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca
     5161 ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt
     5221 gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata
     5281 gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc
     5341 ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa
     5401 gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga
     5461 tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt
     5521 ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc
     5581 gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata
     5641 gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga
     5701 ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa
     5761 ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat
     5821 tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga
     5881 aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct
     5941 tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg
     6001 gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa
     6061 attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa
     6121 ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat
     6181 attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa
     6241 cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag
     6301 caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga
     6361 agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat
     6421 tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc
     6481 ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt
     6541 ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc
     6601 cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt
     6661 cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg
     6721 aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc
     6781 aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc
     6841 taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc
     6901 cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct
     6961 atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa
     7021 agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc
     7081 aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc
     7141 atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact
     7201 ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt
     7261 cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc
     7321 ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag
     7381 tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa
     7441 aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg
     7501 cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt
     7561 ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc
     7621 agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga
     7681 aacagacaaa gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc
     7741 atttgaaatt tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc
     7801 taaacgatcg ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg
     7861 cactgcacca aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt
     7921 ataaataata actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt
     7981 caacacactt tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga
     8041 aagtggctca tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt
     8101 cgtaaataag ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt
     8161 tgttaagtaa tatacaaatt caagataatc gtaacataat cctttatata ataacatagt
     8221 ttattgtgaa aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc
     8281 taattcgtga taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga
     8341 gaggtatcga ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc
     8401 ataataacga tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt
     8461 gctcaaaatt tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac
     8521 aaaagaattt tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat
     8581 cgcttctttt ggtattggta ctttattcag tctgttttct tttctattca gattaagaat
     8641 ctgcttggtc aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc
     8701 gcaatacaga agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct
     8761 agcttctctg cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa
     8821 aagttctgca tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa
     8881 aagagaaatc tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa
     8941 tgaatattgc ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg
     9001 gttcgctgta gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata
     9061 atcttgtttt tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct
     9121 caaataatca gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag
     9181 ggaagaacga ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg
     9241 ctttaacttt tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata
     9301 gtgttcattt tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat
     9361 cttaggaagc aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt
     9421 tttagttatt ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt
     9481 agctatgtgg ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc
     9541 tctttctttt atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt
     9601 tattcaataa tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa
     9661 tagttggtac ttcattactt t
//
LOCUS       DAZKNM010000100         9666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000100 DAZKNM010000000
VERSION     DAZKNM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.101"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8M9_001932"
     CDS             33..656
                     /locus_tag="ACG8M9_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378664.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8M9_001933"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8M9_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0378665.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8M9_001934"
     CDS             2152..3033
                     /locus_tag="ACG8M9_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378666.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8M9_001935"
     CDS             3036..3806
                     /locus_tag="ACG8M9_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378667.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8M9_001936"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8M9_001936"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0378668.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8M9_001937"
     CDS             5117..6280
                     /locus_tag="ACG8M9_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0378669.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8M9_001938"
     CDS             6397..6807
                     /locus_tag="ACG8M9_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0378670.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8M9_001939"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8M9_001939"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0378671.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8M9_001940"
     CDS             9380..9664
                     /locus_tag="ACG8M9_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378672.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3357 a         1639 c         2079 g         2591 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaaga
//
LOCUS       DAZKNM010000101         9644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000101 DAZKNM010000000
VERSION     DAZKNM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.102"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8M9_001941"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8M9_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0378673.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACG8M9_001942"
     CDS             1995..4661
                     /locus_tag="ACG8M9_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0378674.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACG8M9_001943"
     CDS             5025..5777
                     /locus_tag="ACG8M9_001943"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0378675.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACG8M9_001944"
     CDS             complement(5799..6953)
                     /locus_tag="ACG8M9_001944"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0378676.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACG8M9_001945"
     CDS             7138..9579
                     /locus_tag="ACG8M9_001945"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0378677.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3111 a         1736 c         2066 g         2731 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tttt
//
LOCUS       DAZKNM010000102         9597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000102 DAZKNM010000000
VERSION     DAZKNM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.103"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8M9_001946"
     CDS             99..746
                     /locus_tag="ACG8M9_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0378678.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8M9_001947"
     CDS             1139..3784
                     /locus_tag="ACG8M9_001947"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0378679.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8M9_001948"
     CDS             4094..5407
                     /locus_tag="ACG8M9_001948"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0378680.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8M9_001949"
     CDS             5397..6050
                     /locus_tag="ACG8M9_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0378681.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8M9_001950"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8M9_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0378682.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8M9_001951"
     CDS             6947..7147
                     /locus_tag="ACG8M9_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0378683.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8M9_001952"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8M9_001952"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0378684.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8M9_001953"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8M9_001953"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0378685.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         3063 a         1710 c         2041 g         2783 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgacttt
//
LOCUS       DAZKNM010000103         9571 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000103 DAZKNM010000000
VERSION     DAZKNM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.104"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..1060
                     /locus_tag="ACG8M9_001954"
     CDS             191..1060
                     /locus_tag="ACG8M9_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0378686.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1269..2294
                     /locus_tag="ACG8M9_001955"
     CDS             1269..2294
                     /locus_tag="ACG8M9_001955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0378687.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2333..3283
                     /gene="manA"
                     /locus_tag="ACG8M9_001956"
     CDS             2333..3283
                     /gene="manA"
                     /locus_tag="ACG8M9_001956"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0378688.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3377..5080
                     /locus_tag="ACG8M9_001957"
     CDS             3377..5080
                     /locus_tag="ACG8M9_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0378689.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5082..7115
                     /locus_tag="ACG8M9_001958"
     CDS             5082..7115
                     /locus_tag="ACG8M9_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0378690.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7126..7566
                     /locus_tag="ACG8M9_001959"
     CDS             7126..7566
                     /locus_tag="ACG8M9_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0378691.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7766..8893)
                     /locus_tag="ACG8M9_001960"
     CDS             complement(7766..8893)
                     /locus_tag="ACG8M9_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378692.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8896..>9571)
                     /locus_tag="ACG8M9_001961"
     CDS             complement(8896..>9571)
                     /locus_tag="ACG8M9_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0378693.1"
                     /translation="TLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTLL
                     NGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYWQ
                     SKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDPG
                     SIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAIY
                     RKLYEV"
BASE COUNT         3247 a         1658 c         1953 g         2713 t
ORIGIN      
        1 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata agagttttta
       61 ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt ttaacaaaat
      121 caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga cgtagagggg
      181 agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa gtgtagccat
      241 tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg gctttgattt
      301 gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa gtgaaatgtt
      361 gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa agctgaaccg
      421 tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc gtatccgtaa
      481 aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga ttttgctcgt
      541 agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg accaaagaga
      601 aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa aattcagtaa
      661 actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact taacaaaaga
      721 acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag agggcatcca
      781 tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag gcgcaattga
      841 tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg atactgaaag
      901 catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct tagacaaaaa
      961 gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg tcagtgagcc
     1021 aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt cttaacgatt
     1081 gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga gatagaaaga
     1141 gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg tgtaaaaaag
     1201 gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata gaaaagcagg
     1261 agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa gaaggatttt
     1321 atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt acagccggct
     1381 ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt gatgcagcct
     1441 cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa gatggaaaga
     1501 tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag acaatcactt
     1561 tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac agagataagc
     1621 tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag caactaggaa
     1681 aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta gaggaaattt
     1741 accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta ggaatgatga
     1801 acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa ctgccttcgg
     1861 gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct gaacatgcag
     1921 gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa atgtatttga
     1981 acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat tctttactgc
     2041 aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta aaaaaggatg
     2101 tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt ttcttgattg
     2161 atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct gtcgatcatc
     2221 gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa gagaaaacgg
     2281 tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa ttatgcagga
     2341 acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc gtttacgttc
     2401 agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga tcagtgcgca
     2461 tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat tagatgaact
     2521 atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc cattattgat
     2581 caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg atgcctatgg
     2641 gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg atgctgaacc
     2701 tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag ctgagatgat
     2761 caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag gtgatttctt
     2821 ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt tagaaacaca
     2881 gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg cgcaaggaca
     2941 aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg cgaaaacacc
     3001 agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt atttagaaac
     3061 tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca aaaaacaagc
     3121 cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg acggaaaaac
     3181 atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg agtgggcggt
     3241 ccaaggtgaa ctagcaatca ttgcgactga accaggcaaa tagttcccag atagctgaaa
     3301 gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat caataaatag
     3361 aagtatggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc gcgtgtctat
     3421 gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa atctaagtct
     3481 gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg atttgcacac
     3541 cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct tgatcagtta
     3601 ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa ttgcttcttt
     3661 agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta tcgggccgct
     3721 tgccggcatc gtatatgaac aatttgtttc tagattccac gtgaaattca aggctggata
     3781 tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt gtataattgg
     3841 aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct ccgttgtgtc
     3901 ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac ctctaaaagt
     3961 actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa tcgaattctc
     4021 acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac ctggtttagg
     4081 gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg cgagtggagc
     4141 attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg tcttgctgcg
     4201 accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga tatttcaatg
     4261 gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga tttttgccaa
     4321 tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta catttgtgac
     4381 cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga acaaaacggt
     4441 caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt ttgcttgcga
     4501 ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat tagcagcaga
     4561 aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc aagtaaatca
     4621 gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac ctcaaagtca
     4681 aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa taaaagaagc
     4741 gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag aacaaaaaaa
     4801 gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac aaactatggg
     4861 catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta tctcaaaaga
     4921 atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa gagaactagc
     4981 tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac attgggtagc
     5041 tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta tcgcccagag
     5101 aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc caaaaaatga
     5161 tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat cttgaagtct
     5221 cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt gtcattgagg
     5281 catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt aatgacttat
     5341 ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca gagccgttga
     5401 gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat gcagatatca
     5461 aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac cagggatacg
     5521 agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta gaattagaaa
     5581 tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat tatttttttc
     5641 gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa gaattgatcc
     5701 aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg ctgacattga
     5761 cgatggagcg tgtgactaaa aatcatttac tgacaaatga gacatataat agtctggcaa
     5821 acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct gagacaaaac
     5881 aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac ttcacgaact
     5941 catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat ttagtaaaac
     6001 aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat gaaactttgt
     6061 acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta caagaagaga
     6121 cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt gcgaatgcaa
     6181 tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa gagatcgctt
     6241 atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc aatatcagta
     6301 ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta cgattgaaaa
     6361 ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta aaaaaaatcg
     6421 atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga tacggtggaa
     6481 aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta aaagaagctt
     6541 tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat aacgagtgga
     6601 tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat caattactcg
     6661 gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg attcgcagct
     6721 tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat cgccggctgc
     6781 ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg ttattccata
     6841 catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa catccgttga
     6901 ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt atgctggcgc
     6961 cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga gcaatcatca
     7021 tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt tatcagttgt
     7081 tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat acagctacca
     7141 gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt gaccgagatc
     7201 ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc aatgatcgaa
     7261 agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca tgggactgac
     7321 aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga tggtgtggac
     7381 tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc tttaaaaaaa
     7441 gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat cgaccaagta
     7501 atggcgctca gtgatgcgaa aaccattgaa gaagtccaaa tgatttttga acatggacat
     7561 atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac ttcaaccaag
     7621 ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat gcaagctttt
     7681 gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac aaggtttttg
     7741 tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata gtgcccatac
     7801 ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc atgaaagtaa
     7861 cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt tgaaaatcaa
     7921 cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa gaaaacaagc
     7981 gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga gaatgttcaa
     8041 ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac aacaggaaca
     8101 gaaagctctg gaaaagatac tcatacatcg gaaggattcg tatctcctga ccaatgatcg
     8161 ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt atagagacga
     8221 tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta aaacggatgc
     8281 tatgtttctt taccatacta tccggtaata gattgactag agagattacc tgctttcttt
     8341 tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc aaaaaactag
     8401 aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agggagatgc gctggcaagt
     8461 caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac tcgacacgtt
     8521 tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga tccagtatat
     8581 ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg ttttttgcat
     8641 gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat tctttctcat
     8701 tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta tgattcagtt
     8761 catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca aagagcacga
     8821 ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg cgttcaaatg
     8881 taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa tctcttccat
     8941 atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag aacaaatcga
     9001 ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt tctgccattg
     9061 tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca acggttcatc
     9121 gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat gttgtttcat
     9181 tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca atcgttggaa
     9241 aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca aataatcaac
     9301 agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag accggcgata
     9361 atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag ttgttggcag
     9421 cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg caatcaaagc
     9481 agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt ttttaccacg
     9541 tttcatgttc atgtttttga tcagtaatgt c
//
LOCUS       DAZKNM010000104         9570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000104 DAZKNM010000000
VERSION     DAZKNM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.105"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..818
                     /locus_tag="ACG8M9_001962"
     CDS             <1..818
                     /locus_tag="ACG8M9_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0378694.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            938..1138
                     /locus_tag="ACG8M9_001963"
     CDS             938..1138
                     /locus_tag="ACG8M9_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0378695.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1214..1411
                     /locus_tag="ACG8M9_001964"
     CDS             1214..1411
                     /locus_tag="ACG8M9_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378696.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1708..3255)
                     /locus_tag="ACG8M9_001965"
     CDS             complement(1708..3255)
                     /locus_tag="ACG8M9_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0378697.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3339..4142)
                     /locus_tag="ACG8M9_001966"
     CDS             complement(3339..4142)
                     /locus_tag="ACG8M9_001966"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0378698.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG8M9_001967"
     CDS             complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG8M9_001967"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0378699.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG8M9_001968"
     CDS             complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG8M9_001968"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0378700.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG8M9_001969"
     CDS             complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG8M9_001969"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378701.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6527..7456)
                     /locus_tag="ACG8M9_001970"
     CDS             complement(6527..7456)
                     /locus_tag="ACG8M9_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0378702.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7651..8334
                     /locus_tag="ACG8M9_001971"
     CDS             7651..8334
                     /locus_tag="ACG8M9_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0378703.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8495..9538
                     /locus_tag="ACG8M9_001972"
     CDS             8495..9538
                     /locus_tag="ACG8M9_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0378704.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2931 a         1956 c         1900 g         2783 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
      301 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
      361 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
      481 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
      601 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
      721 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
      781 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
      841 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      901 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
      961 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     1021 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     1081 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     1141 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     1201 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     1261 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     1321 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     1381 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     1441 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     1501 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     1561 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     1621 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     1681 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     1741 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     1801 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     1861 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     1921 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     1981 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     2041 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     2101 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     2161 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     2221 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     2281 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     2341 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     2401 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     2461 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     2521 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     2581 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     2641 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     2701 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     2761 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     2821 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     2881 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     2941 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     3001 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     3061 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     3121 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     3181 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     3241 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     3301 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     3361 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     3421 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     3481 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     3541 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     3601 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     3661 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     3721 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     3781 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     3841 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     3901 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     3961 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     4021 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     4081 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     4141 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     4201 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     4261 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     4321 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     4381 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     4441 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     4501 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     4561 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     4621 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     4681 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     4741 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     4801 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     4861 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     4921 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     4981 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     5041 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     5101 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     5161 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     5221 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     5281 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     5341 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     5401 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     5461 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     5521 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     5581 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     5641 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     5701 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     5761 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     5821 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     5881 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     5941 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     6001 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     6061 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     6121 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     6181 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     6241 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     6301 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     6361 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     6421 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     6481 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     6541 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     6601 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     6661 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     6721 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     6781 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     6841 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     6901 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     6961 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     7021 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     7081 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     7141 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     7201 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     7261 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     7321 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     7381 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     7441 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     7501 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     7561 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     7621 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
     7681 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
     7741 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
     7801 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
     7861 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
     7921 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
     7981 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
     8041 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
     8101 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
     8161 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
     8221 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
     8281 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
     8341 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
     8401 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
     8461 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
     8521 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
     8581 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
     8641 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
     8701 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
     8761 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
     8821 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
     8881 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
     8941 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
     9001 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
     9061 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
     9121 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
     9181 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
     9241 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
     9301 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
     9361 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
     9421 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
     9481 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
     9541 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DAZKNM010000105         9516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000105 DAZKNM010000000
VERSION     DAZKNM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.106"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..973
                     /gene="gnd"
                     /locus_tag="ACG8M9_001973"
     CDS             71..973
                     /gene="gnd"
                     /locus_tag="ACG8M9_001973"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0378705.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            975..1976
                     /locus_tag="ACG8M9_001974"
     CDS             975..1976
                     /locus_tag="ACG8M9_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0378706.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1973..2257
                     /locus_tag="ACG8M9_001975"
     CDS             1973..2257
                     /locus_tag="ACG8M9_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0378707.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2290..3087
                     /locus_tag="ACG8M9_001976"
     CDS             2290..3087
                     /locus_tag="ACG8M9_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0378708.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3101..3934
                     /locus_tag="ACG8M9_001977"
     CDS             3101..3934
                     /locus_tag="ACG8M9_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0378709.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3952..4809
                     /locus_tag="ACG8M9_001978"
     CDS             3952..4809
                     /locus_tag="ACG8M9_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0378710.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4831..5340
                     /locus_tag="ACG8M9_001979"
     CDS             4831..5340
                     /locus_tag="ACG8M9_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0378711.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5377..5778
                     /locus_tag="ACG8M9_001980"
     CDS             5377..5778
                     /locus_tag="ACG8M9_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378712.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5890..6243
                     /locus_tag="ACG8M9_001981"
     CDS             5890..6243
                     /locus_tag="ACG8M9_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378713.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6277..6720
                     /locus_tag="ACG8M9_001982"
     CDS             6277..6720
                     /locus_tag="ACG8M9_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0378714.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6737..7822
                     /gene="uxuA"
                     /locus_tag="ACG8M9_001983"
     CDS             6737..7822
                     /gene="uxuA"
                     /locus_tag="ACG8M9_001983"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0378715.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7815..8528
                     /locus_tag="ACG8M9_001984"
     CDS             7815..8528
                     /locus_tag="ACG8M9_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0378716.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8735..8884)
                     /locus_tag="ACG8M9_001985"
     CDS             complement(8735..8884)
                     /locus_tag="ACG8M9_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378717.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9280..9480
                     /locus_tag="ACG8M9_001986"
     CDS             9280..9480
                     /locus_tag="ACG8M9_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0378718.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3199 a         1494 c         2110 g         2713 t
ORIGIN      
        1 tatttttgta aaatctattt acaaaaaaat ttttttgtat aatgagggta taagaaatgg
       61 aggcgctatc atggaaattg gtgtaattgg tttgggaaaa atgggattga atttagcttt
      121 aaacttaaaa gagcaccatc acaaagtaca aggactcgac atttcaagta cagctgttag
      181 tcaggcaaga gaaaatgaca ttcctgctta tcaagagatc tctgaatttc tagctacttt
      241 tgagcagaaa aaaatcatct ggttaatgct tcctgctgga gagataaccg aaaacatgtt
      301 aagaaaactt tttccaaaac tcggaccagg agacattatt attgaaggcg gaaactctta
      361 ttataaagat tcaatccgtc gagcagaaga gtttcagaaa aatgaaatcg gttacttgga
      421 ttgtgggact tctggaggaa tcgaaggagc acgacatcaa gcctgcttaa tgattggagg
      481 agataaagaa acttttctat ccgtggaaca attattcaaa gatgtagcaa ttgaaagagg
      541 atatctgtat gctggtgcat ccggaagcgg acacttcttg aaaatgatcc ataatggaat
      601 cgaatatgga atgatgcaag caatcggtga aggattccaa cttgtacaaa aaagtgagta
      661 tgcttttgac ttggaacaag tcgcacgtgt ttggaatcat ggttcggtga ttcgcggctg
      721 gctgatggaa atcatggaag atcagttaaa cgaacatccc aatttaagta attatcgggg
      781 aatcgtttct gcatcaggtg aagcaaaatg gacggttgaa acagcgctag aaatggctgt
      841 tccagttcca gtaattgcaa ctagtttgtt catgaggaat ctttcacagg aagaagatag
      901 tttttcagca aaagtagttt cagcactaag aaatgggttt ggcggacatg aaatagttga
      961 aaagggtgag taaaatgcca gattatcata tagcaatcgt taattccagc agtttcggaa
     1021 gagtctttcc acaccacttg gaaagattaa agtcagttgg agacgttcat ttctttactg
     1081 ttgatcaaga gattccagga aaagaactag cggagttact taaaggattc aatatcatta
     1141 ttgcaagtgt tacgccattt tttcgacgag atttttttga aaacaaagat gaactgctat
     1201 tgatttcccg acatggtatt ggttacaaca atattgattt aaaagcagca aaagaacacc
     1261 agacaatcgt atctatcata cctgctttag ttgaacgaaa tgctgtagca gaaaataata
     1321 taactaattt gttgtccatg cttcgacgca cgacacaagc acaagcaaga gtcaaggacg
     1381 atcgatggga agatcgggcg gagttcgtcg gtaggacgct gtttaacaaa acagttggtg
     1441 tgattggtgc aggaaatacc ggaagcggag taatcgaaat tttgcgtaac ggttttcgct
     1501 gtgatgttct agcttatgat ccttacaaat cagatttata catcgaaagc tttggtgcaa
     1561 aaaaagtaga acttcaagaa ttactggaac aatcagatat catttgttta tgtgcgaatt
     1621 tgacagaaga gaactatcac atgatttctg ataaagaagt cgatcagatg aaagaaaatg
     1681 tttatctatc taatagtgca agaggcgctc tagttgatga agaagcgatg attaatggtc
     1741 tgaaaagcgg aaagatcgca ggatatgcca cagatgtttt agaagaagaa ccgggacgtc
     1801 aagatcatcc gtatttagcg tttgaacaag tagtgattac gccacatata tcggcctaca
     1861 caagggaatg tttagaagca atgggtgaaa aatgtgtcag agatgtcgaa cagatcagtc
     1921 agcaccttct tcctgaacgt tctgtccaga caaagagcaa ctatataaaa ggatgatgaa
     1981 gaaaatggtg aacggtttaa aagtgaaaac aggccctcaa ttttatttat atgaagaagg
     2041 tggtatttca aaagtttctg atctcttgaa atcatacggc gcgaaaagag tactggtcac
     2101 ccacggaacg gtatcatggg aaaaggcgct acctaaatta gtatttttga atgacgaaac
     2161 gattcaattt ttttatcatc gttattctgg agaatgtagt tatgctgaag ccagacgaat
     2221 agctacaatc atcaaaaaaa tgaaatcgat ttcgtagttg gcgttggcgg aggaaaactg
     2281 accgatctag tgggttatag tgccaatctt gcaagaatcg atttcggagt catccctact
     2341 ttagctagta attgtgctcc atggacacct cttgctgtca tgtataagga gaatggacaa
     2401 gcagaaggga aaacagaaca cttttacaga caagctgctt tttttataac agatccccaa
     2461 ttagtgattg attcccctgt tccatatttc attgccgggt tagcagatac actagcaaaa
     2521 tggtatgaat ctgagctcat cttagaacaa gattttttgc aagatgaatc atttttgaag
     2581 ctagcacaag atactgcgaa gatctgcaaa gaagagattt tgaatcattc tgctaaagca
     2641 atccaagata tggcacaaag aaaactgaca tcggagttca aacagctttc agagattatc
     2701 tttgctgttg ctggtttagt tggaggatta ggagataaat acgcaagaaa tgtggcagca
     2761 catgctatgc atgacgctat cagtaaatat attccggaaa gccatcgttt tctacatgga
     2821 gaaaaagtag cgtatgggat tttctatcaa ctggcactag aaggtcgctg ggaaaaaata
     2881 gacgagttat atcctttgta tcgggaatta gaactgccgg tttctctaga acaaatggga
     2941 ttatttccga aagataaaca gataatagat aaaatggctg aatttattga ttcgaaagag
     3001 aaagtgcatt tgattcctat agaggtaaca cagaaatcac tgatacagtc aatgatggaa
     3061 ctggaaggtt atatcagaaa gatctgaagg aggaggacac atggaaacat taagtgtagt
     3121 acaaagtttg ttgattgctt tatgggtagc cgcggttatg tcccgctggt taggtggagg
     3181 agccacgttg accttacgtt tttctccttt gatgactgga ttagtcgtcg ggttgatcat
     3241 gggggaagtt gcacaagcga tgattgttac agccgctttg caaatgattt atatgggggt
     3301 tttttctcca ggaggatcaa tgccagctga accatctatt gcagcagcta tcgcagtacc
     3361 agtcgcatta ttaggggatc tgtcgccaga agcagcgatt gccgttgcgg tgccagtcgg
     3421 attgctcgga agttacttgt atcagtttcg ttttttcatc aatacttttt taggtaaata
     3481 tacagacaaa gctgtagaag atttgaatac aaaagctatg gcccgatcaa ttattcttta
     3541 tccaacggtt gcctcatttt tattattcgt tccgcttgtt ttcattgctt tatatttagg
     3601 ggcgccattg atcgctgatg taatcaaagc tttagaggga acagtcgtta tccacattct
     3661 tgaagtagta ggtggagggt tagcggcaat cggtatcgcg acaactgtat acgttatcgg
     3721 tcgaaaagat tatctggtct ttttcttcct tgcttatttt atgagtgttg ttttgaaatc
     3781 tttagatatt acgatggtta cttatgccat ttttgggatt attatcgctt tgatctttgt
     3841 ccagtcgcaa aaagggaaac aagtacagcc tgccacaagc aataacaatt cagcatcttt
     3901 tgatgacgaa gatgactatg atgacggatt ttaatttagg aggaatggga aatggctgaa
     3961 ataatggata atcaaacagg tgctcctgaa gaaatcacga aaaaagatgt aacaaaagcc
     4021 tatctgagat ggcattttgc aaacgaaatt cctcactctt ttgaacgtta cttggcgcca
     4081 tcactactat acggaatgat gccaatcttg aaaaaacttt ataaagatga agactcttta
     4141 aaagcagcat ataaacggca gttactgttc ttcaacactc aattaagctg gggtggcggt
     4201 gtgattactg gcctaatgtc ttctatggaa cagcaaagag cagaagaaga gtatgaagaa
     4261 aaagagatct tgatgcagga tgacttgatg tataacacga aagcgggatt gatgggggca
     4321 ctagcaggaa tcggagatgc aatcgattca ggaaccgtcc aatatatttt tatcgcaatc
     4381 gcggtacctt gggcacaaca gggaagtcct ttaggcgcac tttttccttt tatctgcttt
     4441 gggttgtatc aggttttact aggggtattt ttcgcaagac aagctttcac tatgggccgt
     4501 aatgctacag gcctaaagca aaacactggt gttcagacag cgattgaaat gctatcagtt
     4561 ttagggttgt ttatgatggg ggtgcttgcg ggaaactatg taaaagtaga atcttcattg
     4621 aaatttgcga tatccggacg tgagtttgtt atccaggaaa ttcttgatca aatcgtgcca
     4681 gggatattac ctttagctgt cgtaatgggt gtgtacttgt tctatacgaa aaaaggattg
     4741 aaagtcacac aagcattatt atggctgact ggaatattaa ttgtgctagc aggaatcggt
     4801 attttataaa aagaagggaa cgtgaataag atgggtgtag tcaatttagc gcgtgtggat
     4861 gagcgtttga tccatgggca agtaatggta acgttatctc aaaaaagtgg agtgaattcc
     4921 atttttgttg tagacgaagt tgtagcaaaa gataaattta tgagagatct ttacaaaaat
     4981 gcaggaagcc gtacaggaca aaaaacaatt gtcattacac cagagaaagc taaattctat
     5041 tgggatgaat atcaatttaa agaatacaat tgtattttga tcgcaaaaac agtccaaacg
     5101 atttacgact tagtaaaaca cggaatcccc atggaagaat tgaatattgg agggattgct
     5161 caaaaaaatc ctgatacaga tcttttagtg acgaaatctg tttatttaaa taaatcagat
     5221 gctgaaaaat tgaaagaaat gaatgaacaa tatggcgtaa agaatattta ttttcaagcc
     5281 actccatctg cacctaaaac aggattaaaa gatgtattga acaagtttga tttgtcataa
     5341 acaaatgctt agaaaaaaga taaagagggc gaaaaaatga agaagaacga gctgtttcgt
     5401 gattgggaat ttcgttatcg ctatatttac cgtaagcgcc gtacaaaaaa gagtaaacag
     5461 cgttttcttt cagcattagt atcagatatt tacagtatga gaacagatgt gacagtcatt
     5521 gcttacgata cgctagccta ccgatcaaaa aatatttatg ttggagatat cgaaaaagca
     5581 gagaaagtta tctgtactta ttacgataca cccgttcatg cattaggaag ttatttcatg
     5641 tttgattgga aagaccaaag gaaaaaaaca atctactcta tattattgtc cttcattttg
     5701 ttgttcagtt taggttggtg gggaatgatg atatataaca aaaatcctca tcacgtattc
     5761 gatctgctat ccgtataaac aggtatcact gtactagcat ttgggagcta ctttttctta
     5821 ttgggaaaag cagctcgcgg atggcctagc agacaaactt ttattcgaaa tacttcatcc
     5881 atattaacga tgctagaaat gatccgtacg attgatgatc cgagtgtagc gtatgcgttt
     5941 gttgatgaag gctgttatgg agaaaagggc ttagacagcg tgagatcggg tatgaaaaaa
     6001 gaggctattc ttttttatct agactctgtt ggggcagata caccgctaca gttttcggga
     6061 aattattttt caaataaaga acagtggctg aaacaagttg ataagttgaa agaaaaaaac
     6121 gtcaattata tttttagtgc aagaaaaaag caagcgcagt tcttctattt gaccaaaaca
     6181 gatttacgag ggaaaacatt taattggcaa aatgccaatc agattattgc tctttttcga
     6241 taagtaaaaa gtgcaaaaca aggagtgaga agactaatgt tggaaatcat gatcgttaca
     6301 catggtaagt tgagcgaagg actgaaagat gcggcggaag taattgtcgg gacatcgagc
     6361 catattgaaa caatgtgctt agagtctggg gaggacatcc aaacattcgg agagaaggtc
     6421 aaagaaaaat taactatgat caacacagca gaacccatgc tagtatttgt tgatctaatt
     6481 agcgcaagtc cgtacaatca agcgctcttg gcagtcaatg aactgactga agaaaagaaa
     6541 gcagccactt atgtcatagg gggcgtgaat ctgccaatgt tgttagaagc aatcaatcat
     6601 cagatgcttc acacaccgat agaagaagcg gtggaacaaa ttgtcaaaca aggaaaagat
     6661 aatttaactg tttggcatgc ttcagcggaa gaaaaaaaat tagatgagga cgatttttag
     6721 aaggaaggct gaaaagatga agtggggatt tagatggtat ggtgaaaaag gggattcgat
     6781 cccattaaaa tatgttcgcc aaataccggg aataaatggg ataattggaa ctttgctgac
     6841 taaaatgcct ggagatgttt gggagatttc agaaattgag gagttgaaag catctgttga
     6901 gagccaagga ctggaactgc ttggaattga aagtgtagcg atccacgatg caatcaaggc
     6961 agggacagat gaacgagatt actatattga taactatata cgaacaatca aaaacttgtc
     7021 tcgatgtggg gttcgattga tttgttatag cttcaaacca atttttggat gggcgaaaac
     7081 tgatttgttt aatgaaaaca acgatggaag ctattctctt ttatacgatc agaaaatcgt
     7141 cgatcagatg gagccaggag aaatgtatca attgatacac agccagtcaa agggattcaa
     7201 attgccgggt tgggaagaag aacggttgaa aaaattcgct cagctggaaa aactgtatga
     7261 aggaatgacc gaagaagaat tatttgaaaa tctgacttat tttttagaaa aagtgattcc
     7321 agtgtgcgaa aaagcagatg taaaaatggc tatccatcca gatgatccgc catgggaact
     7381 ttttggtttt cctagagtga cgaaaaatct tggcgacttg aagaaaattc taaatgcagt
     7441 agattctcca tataatggac taacgctgtg cacaggatct ttaggggcaa atccggaaaa
     7501 cgatttagtt gaaatcatcc atgaggtagg ttcgcgcatc cattttgtcc acttcagaaa
     7561 cgtggagttt ttaggagaaa gaaagttcaa agaaacggca catccctcta cagaaggctc
     7621 ccttgatatg tatcatatta tgaaagcgtt ggtgcaagaa gggttcgacg gtcctatacg
     7681 ccctgatcat ggtagaaccg tgtggggtga agtggctatg cctggatatg gcctgtatga
     7741 tcgtgcaatg ggactcactt acatgcaagg tctatatgag gcgataagta aggaaaaacc
     7801 actgggagag cattatgaat aaaatcaagc tcttcatctt tgatatggat ggccttttat
     7861 tagaaactgg acgtttagct tatcgtgctt atgtaaaatc agcacaaaag tatgattatg
     7921 aaatgcgtaa ggaagtctat tatttactaa ctggccaaac agaaatggcg ataagagaac
     7981 aaatgggcat tttgtacgga gaagatgttc catatatcaa atggcgagaa gcaatcaatc
     8041 aatataaaga aaaaatcgtg aaagaagata agagagtata tacgaaaaaa ggagcagaag
     8101 aaattttatc ttttgcaaaa gaaagaggaa tccatacaat cgtggcttcg tctaatacac
     8161 gggtaaaagt agaaatgtat ttaaaaatgg aaaatctata tgccttgttt gatcaaatca
     8221 tttcagggga tgatgtaaaa aaaggaaagc cggaaccaga gatctttttg aaagcttgtt
     8281 caaaaatgaa tattcctcca agtgaagcac ttgtatttga agactcgatt gcagggatcg
     8341 aagcagcaag gcgagcgggc attttgtctt ttctcatcaa agatcatttg atagggttgc
     8401 ccgatcataa tgggaagcat aagttgaaag tagatgtaag tttatatgaa tccaataaag
     8461 cagactatga attttatgac ctgcatcaag caaaacgatt tttagaaagt aaagggttgt
     8521 atctctaata aagaggacta ttttctagtc ctctttattt attgtcctgc acgactgagg
     8581 gaacagatga aaaagaagta attttttatt ttgaagtatc taccttattt gtagtgaaca
     8641 atttagaaca aacacaaaac gcatctactg ttatctacca gtagatgcgt ttaaagagaa
     8701 gaacgagaat cgtccatatg agggtttcca ctccttatgc agattttttt aattcttgca
     8761 ctgcttgttc gatcgtaaca cctgtcgcta catgattggt attttctttg tcaaaaacga
     8821 caaatagatt caaatctgta tccaaggtta ctcgatattg tatatctttg ttatagaatg
     8881 tcatagccat tctcctttct ttaaatactt gctgaaaggc aacgaacgcg ttgataacga
     8941 aattccattt ataagtatac caccaattga aaggttttca tagttaagaa attttaaagt
     9001 caaaagttgc agataaaagc aaataaacaa gaaaacaaaa agtgaatcaa tcgcggttat
     9061 tcaacttttt cttcgattcg aagacaatag aagacaatga aatttatatt tcattgcatt
     9121 atccttgtgt tattagaaaa agcatgatat attaaatgag taatctattt tgaaacgtaa
     9181 tttgagcgat atatctacat agataaaact ccttttgcct atcaaatatt tattgcaacg
     9241 aaaaaacgta cattgatacg tatcaggagg aatttattta tgaataacgg tacagtaaaa
     9301 tggttcaacg cagacaaagg ttttggcttt atcactggtg aagatggaaa tgatgtattt
     9361 gcacattttt cagctatcca aggtgacggc ttcaaaactt tagaagaagg ccaagcagtg
     9421 tcttacgatg ttgaagatgg tcaacgtggc cctcaagcaa caaacattgt taaagcttaa
     9481 taaaaattga cttttaaccg tcttttcgaa agaaaa
//
LOCUS       DAZKNM010000106         9256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000106 DAZKNM010000000
VERSION     DAZKNM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.107"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..655)
                     /locus_tag="ACG8M9_001987"
     CDS             complement(<1..655)
                     /locus_tag="ACG8M9_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0378719.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     "
     gene            789..1076
                     /locus_tag="ACG8M9_001988"
     CDS             789..1076
                     /locus_tag="ACG8M9_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378720.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1492..3030
                     /locus_tag="ACG8M9_001989"
     CDS             1492..3030
                     /locus_tag="ACG8M9_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0378721.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3027..3992
                     /locus_tag="ACG8M9_001990"
     CDS             3027..3992
                     /locus_tag="ACG8M9_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378722.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3979..5301
                     /locus_tag="ACG8M9_001991"
     CDS             3979..5301
                     /locus_tag="ACG8M9_001991"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0378723.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5364..5468
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5542..6285
                     /locus_tag="ACG8M9_001992"
     CDS             5542..6285
                     /locus_tag="ACG8M9_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0378724.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6300..7292
                     /locus_tag="ACG8M9_001993"
     CDS             6300..7292
                     /locus_tag="ACG8M9_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0378725.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7304..8041
                     /locus_tag="ACG8M9_001994"
     CDS             7304..8041
                     /locus_tag="ACG8M9_001994"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378726.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8031..8753
                     /locus_tag="ACG8M9_001995"
     CDS             8031..8753
                     /locus_tag="ACG8M9_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0378727.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8837..>9256)
                     /locus_tag="ACG8M9_001996"
     CDS             complement(8837..>9256)
                     /locus_tag="ACG8M9_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0378728.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3026 a         1599 c         1959 g         2672 t
ORIGIN      
        1 gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca
       61 aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc
      121 taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac
      181 caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt
      241 gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa
      301 tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta
      361 tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc
      421 ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa
      481 gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag
      541 taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga
      601 ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta
      661 gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact
      721 tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga
      781 gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag
      841 agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga
      901 taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga
      961 aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag
     1021 aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa
     1081 aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa
     1141 aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg
     1201 agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag
     1261 atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa
     1321 cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta
     1381 gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg
     1441 gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat
     1501 tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg
     1561 tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg
     1621 gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg
     1681 attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca
     1741 gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt
     1801 ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg
     1861 agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga
     1921 actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga
     1981 ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt
     2041 gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat
     2101 aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt
     2161 tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga
     2221 gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca
     2281 gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa
     2341 ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta
     2401 cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa
     2461 ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat
     2521 gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg
     2581 gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat
     2641 ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag
     2701 ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc
     2761 acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc
     2821 ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc
     2881 gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat
     2941 taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat
     3001 gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa
     3061 aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa
     3121 tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg
     3181 gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata
     3241 atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga
     3301 ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga
     3361 aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc
     3421 gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag
     3481 ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga
     3541 tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca
     3601 tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta
     3661 tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa
     3721 acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag
     3781 atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt
     3841 gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga
     3901 tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa
     3961 gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc
     4021 ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa
     4081 gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt
     4141 gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca
     4201 actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa
     4261 agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt
     4321 ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg
     4381 gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt
     4441 gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat
     4501 attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat
     4561 cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa
     4621 atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa
     4681 ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt
     4741 gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt
     4801 gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg
     4861 tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga
     4921 aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata
     4981 cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc
     5041 tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc
     5101 aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct
     5161 gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa
     5221 aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat
     5281 ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct
     5341 tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt
     5401 aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag
     5461 cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat
     5521 tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc
     5581 attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc
     5641 ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct
     5701 gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt
     5761 actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg
     5821 atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc
     5881 ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct
     5941 ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg
     6001 gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt
     6061 tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta
     6121 agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat
     6181 aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt
     6241 tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa
     6301 tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa
     6361 aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt
     6421 atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag
     6481 aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag
     6541 taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg
     6601 cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg
     6661 gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat
     6721 atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg
     6781 gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag
     6841 gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag
     6901 cgggtattga actaaactat atcccattga aagaattaga tgaacggtta gattactata
     6961 ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt
     7021 ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta
     7081 aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat
     7141 tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt
     7201 ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc
     7261 aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag
     7321 ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc
     7381 taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa
     7441 aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt
     7501 aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct
     7561 gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga
     7621 acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga
     7681 acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc
     7741 ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat
     7801 gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca
     7861 tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc
     7921 acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca
     7981 gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg
     8041 atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga
     8101 ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag
     8161 caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt
     8221 ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata
     8281 atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt
     8341 ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct
     8401 taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact
     8461 atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga
     8521 atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg
     8581 atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct
     8641 cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag
     8701 ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag
     8761 atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt
     8821 gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc
     8881 aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt
     8941 agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg
     9001 ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg
     9061 ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg
     9121 cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt
     9181 cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta
     9241 attgttgtcc tgagta
//
LOCUS       DAZKNM010000107         9113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000107 DAZKNM010000000
VERSION     DAZKNM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.108"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..863)
                     /locus_tag="ACG8M9_001997"
     CDS             complement(39..863)
                     /locus_tag="ACG8M9_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0378729.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(836..1516)
                     /locus_tag="ACG8M9_001998"
     CDS             complement(836..1516)
                     /locus_tag="ACG8M9_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378730.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1539..2477)
                     /locus_tag="ACG8M9_001999"
     CDS             complement(1539..2477)
                     /locus_tag="ACG8M9_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0378731.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2722..3573)
                     /gene="ispE"
                     /locus_tag="ACG8M9_002000"
     CDS             complement(2722..3573)
                     /gene="ispE"
                     /locus_tag="ACG8M9_002000"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0378732.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3727..6300)
                     /gene="mprF"
                     /locus_tag="ACG8M9_002001"
     CDS             complement(3727..6300)
                     /gene="mprF"
                     /locus_tag="ACG8M9_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0378733.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6441..7247
                     /gene="proC"
                     /locus_tag="ACG8M9_002002"
     CDS             6441..7247
                     /gene="proC"
                     /locus_tag="ACG8M9_002002"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0378734.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7326..8882)
                     /gene="rny"
                     /locus_tag="ACG8M9_002003"
     CDS             complement(7326..8882)
                     /gene="rny"
                     /locus_tag="ACG8M9_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0378735.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2683 a         2015 c         1569 g         2846 t
ORIGIN      
        1 aaatttttcg tttttataag gcttcttttc tttataattt agttattttt tcttctgaag
       61 gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa gactaatgtg
      121 attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga cagcatccct
      181 agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc taatcgcata
      241 ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc aatcgggatc
      301 atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa atgaacgggt
      361 agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg ccgtttgaat
      421 aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac ttgctcccat
      481 gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga attccctccg
      541 ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat ggcaatggag
      601 atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc tgctaataca
      661 acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa cgctacccct
      721 gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat cactaaaaac
      781 acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc tcttctcatg
      841 aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg atctgtctgt
      901 ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat ttcccatgga
      961 catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca tccatacctg
     1021 ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc gctaaactga
     1081 ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata tcccacatcc
     1141 ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga cgcttgaacc
     1201 agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta ggaaaacctg
     1261 cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt ccttcttgat
     1321 ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac gatgctttca
     1381 ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca cccgcatcta
     1441 catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc aaatcagata
     1501 tttcgatata acgcacctta caacctccat tctaaaactt aattgatact ttcttttaaa
     1561 gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat ttgttcatta
     1621 gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt cgacaatgtc
     1681 tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt gtttttcttc
     1741 acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg tgaaacgccc
     1801 gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc agcatgctga
     1861 gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg atccagtgct
     1921 tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt atctggatat
     1981 ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac cggatcgagc
     2041 caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc ttcatgttcg
     2101 tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat caaggtctgt
     2161 ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga actattatac
     2221 acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc gaaatcatgc
     2281 ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac gatctctttt
     2341 gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt tttgtctgct
     2401 tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac cgtagctgtc
     2461 aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt ttacgattta
     2521 ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc atcaatttct
     2581 gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg ctcatatcta
     2641 gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca gtttttcatc
     2701 acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt cctcacaaaa
     2761 accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca aggcaaaaac
     2821 ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca tacgctcttt
     2881 gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt ttcccatttt
     2941 tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt ggatgtcttg
     3001 atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga cactcattct
     3061 tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga tttcttcccc
     3121 tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg taccgacttc
     3181 caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt ggttgatccc
     3241 tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta ctggaatatt
     3301 cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta gcaataaagc
     3361 agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg tttctacaat
     3421 gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta cgctcgacat
     3481 caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca gtcctaaatt
     3541 tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa aagtctaagt
     3601 cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct agtggttgat
     3661 tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa caagttctag
     3721 tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc ctttttcttt
     3781 tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt tcctataagc
     3841 aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag cagactggaa
     3901 ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt gaaagaaaga
     3961 aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt ccgagtagcc
     4021 cattttctcg tatacaataa acagattggc taacagatag tcagctaatt cctcagattc
     4081 tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg gctgagcggt
     4141 gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag ataatacata
     4201 tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa gccatttttg
     4261 tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc aagtaaaatg
     4321 gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag acaagtcttc
     4381 tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac ccaattcatg
     4441 aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc ctaaacaatc
     4501 tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc caactggatc
     4561 tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt gatcttggtc
     4621 gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc ttgtagctgt
     4681 gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac tgccaagctt
     4741 cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg agatgcccaa
     4801 accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat gatttcctgt
     4861 tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa cgataaacaa
     4921 aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga attttgttcg
     4981 ataaaattct tttctggata accacacagc taacagaatc aagacatata ccaatatcaa
     5041 ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta aagcaatcgt
     5101 aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga gtaataaaaa
     5161 gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca ataatacttg
     5221 aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca tcatgatccc
     5281 cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa acagtcgcgg
     5341 aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca atagaagtcc
     5401 cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa ctactgctac
     5461 atcttgccga acagacaatt gactcattcc taagatcatc aaaacgtcaa aagtccccat
     5521 tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca taggatagat
     5581 cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata ccgcaagggc
     5641 tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg gataaaactc
     5701 gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg ctaaaaaact
     5761 accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa taaaaaagac
     5821 atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata gtgcgacttt
     5881 tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac tagcccgcag
     5941 acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg ttacccacgc
     6001 agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta agactctgat
     6061 cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca gaatcatgag
     6121 caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc aactcatgcc
     6181 attagcaata tttgcgactt gatttgccac aaagactaac acggagccga aaaaaatcag
     6241 ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt tatttttcac
     6301 attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc ttatttattt
     6361 taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa agatatccta
     6421 atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat atggcacaag
     6481 ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg ttcgtttcca
     6541 gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat gcatgcaaaa
     6601 gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa ccaaaaatca
     6661 ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc atttctattg
     6721 cttcaggaaa atcattaata gatttagaaa cagcgcttga agatcctcag gcctccatcg
     6781 tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg atttgcaaaa
     6841 atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa gcaatcggca
     6901 gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct ggcagctcac
     6961 ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg aatggtttgt
     7021 ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt caaaaattcc
     7081 tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct ggcggtacaa
     7141 ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc atcaaaggaa
     7201 tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga tagcaatgtt
     7261 ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt cttttgtatt
     7321 ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac tttgatgtgt
     7381 ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa acgaacagcg
     7441 tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc agcttgaact
     7501 gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact tcttaaacga
     7561 cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga gagcgcatct
     7621 gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg atgagaagcg
     7681 atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc accgatctca
     7741 acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag caacccggca
     7801 cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc taattttgca
     7861 acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg cagacgtccg
     7921 atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc tgcctcttcc
     7981 ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat ttcttcgata
     8041 cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg tgcgatttct
     8101 cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc gatgattaaa
     8161 tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc aatgatacga
     8221 cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga aacttgatct
     8281 gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc tgcttcttct
     8341 ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag ctcttcttca
     8401 gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat tttttctagt
     8461 tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc aattaattgc
     8521 tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt ttctaacgag
     8581 tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg agattttaat
     8641 tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg attttcttcc
     8701 ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc actatcgatg
     8761 attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga ctttgcgacc
     8821 ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat gttgaatacc
     8881 ataaaatcca cctccatact atctttttca tttttatcat ctatacagct tctatgataa
     8941 attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa catgcacaac
     9001 tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa acggctttct
     9061 acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat agg
//
LOCUS       DAZKNM010000108         8942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000108 DAZKNM010000000
VERSION     DAZKNM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.109"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG8M9_002004"
     CDS             <1..260
                     /locus_tag="ACG8M9_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378736.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACG8M9_002005"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACG8M9_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ACG8M9_002006"
     CDS             complement(1379..1984)
                     /locus_tag="ACG8M9_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0378737.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ACG8M9_002007"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ACG8M9_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2881
                     /locus_tag="ACG8M9_002008"
                     /pseudo
     CDS             2666..2881
                     /locus_tag="ACG8M9_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3019..3990
                     /locus_tag="ACG8M9_002009"
     CDS             3019..3990
                     /locus_tag="ACG8M9_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0378738.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ACG8M9_002010"
     CDS             4130..5443
                     /locus_tag="ACG8M9_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0378739.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ACG8M9_002011"
     CDS             5486..6352
                     /locus_tag="ACG8M9_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0378740.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ACG8M9_002012"
     CDS             6349..7197
                     /locus_tag="ACG8M9_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0378741.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ACG8M9_002013"
     CDS             7211..8683
                     /locus_tag="ACG8M9_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0378742.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="ACG8M9_002014"
     CDS             8700..>8942
                     /locus_tag="ACG8M9_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0378743.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAZKNM010000109         8866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000109 DAZKNM010000000
VERSION     DAZKNM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.110"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..1247)
                     /gene="hflX"
                     /locus_tag="ACG8M9_002015"
     CDS             complement(18..1247)
                     /gene="hflX"
                     /locus_tag="ACG8M9_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0378744.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1249..2166)
                     /gene="miaA"
                     /locus_tag="ACG8M9_002016"
     CDS             complement(1249..2166)
                     /gene="miaA"
                     /locus_tag="ACG8M9_002016"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0378745.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2170..2907)
                     /locus_tag="ACG8M9_002017"
     CDS             complement(2170..2907)
                     /locus_tag="ACG8M9_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0378746.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2998..3525)
                     /locus_tag="ACG8M9_002018"
     CDS             complement(2998..3525)
                     /locus_tag="ACG8M9_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378747.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3705..4298
                     /gene="clpP"
                     /locus_tag="ACG8M9_002019"
     CDS             3705..4298
                     /gene="clpP"
                     /locus_tag="ACG8M9_002019"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0378748.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4402..4887)
                     /locus_tag="ACG8M9_002020"
     CDS             complement(4402..4887)
                     /locus_tag="ACG8M9_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0378749.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5040..5237)
                     /locus_tag="ACG8M9_002021"
     CDS             complement(5040..5237)
                     /locus_tag="ACG8M9_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378750.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5332..7092)
                     /gene="efrB"
                     /locus_tag="ACG8M9_002022"
     CDS             complement(5332..7092)
                     /gene="efrB"
                     /locus_tag="ACG8M9_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0378751.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7089..8819)
                     /gene="efrA"
                     /locus_tag="ACG8M9_002023"
     CDS             complement(7089..8819)
                     /gene="efrA"
                     /locus_tag="ACG8M9_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0378752.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2499 a         1971 c         1485 g         2911 t
ORIGIN      
        1 gttaaaaaaa taaaatttta ttggtttttt cttaaccatt ttgaattggt ttttgcaaac
       61 cctttaactt catattcgtt tgtttcttcc atgtacgctt cgtccaaaac cattgtttgt
      121 cttctcaact cgcttaattt ctgtccttcg ttgcttggca cagaaagtgt ataaggggtc
      181 attagttcca tcagctgacg tttgattgct tcgatcaacg ccatcttacc ttctttcgtc
      241 tgagtagaaa tcaatacatt tgggaatagc gtaggcgtga acatcgctgg gtcgatctgg
      301 tctgccttat tgtaaacagt cagtatcggc atttctttca tattcagttc atccatcaat
      361 tttaagacag tctgctcctg taaaatacga tctggagaac tagcatcgac tacatgtagc
      421 aaaagatcca tattttgact ttcttctaat gtagaatgga aagcatcgat cagttgcgta
      481 ggcaggtctt ggatgaaccc gacggtatcc gttacagtaa tctcaaatcc ttcagcaaaa
      541 cgccatctct tagtcaatgg atctaacgtt gcaaacaatt gatctttcga ataagtatcc
      601 gcttgtgtca atagattcaa gatagttgac ttcccggcat tcgtgtagcc aataagtcca
      661 atctgaaaaa tctcgctatt ttttcttttt tggcgattgc gctctcgatg agcttctact
      721 tctttcaatt ctttcttaac agcaaaaatt ttattccgta tatgtcggcg gtctgtttct
      781 aactttgttt cacctggccc tcttgttccg atcccaccgc caagacgaga taatgttttc
      841 ccttgcccta ctaaacgagg cagcaggtat tctaactgag caagttcaac ctgcagcttc
      901 ccttctttcg aacgagcacg catcgcaaaa atatctaaga taagctgcac gcgatcaatt
      961 accggaagac caacagcatc ggtaatcagt tggctctgtc tcggagttag ctcatgatta
     1021 aagatgatca aatccgcttc atgcgcatcc gcttgttgaa ctaattcttg taatttccct
     1081 ttaccgataa tcgtttgccg atccacttgt ggtctctttt gtgtaattgt gaatacgact
     1141 tctcctgatg cggtattcgt cagctgaact agctcttcca ttgactctga aaagtaacgc
     1201 tggtttgctt cggtttctac ccctacgatg atcacttttt cttccatttt attccgcctc
     1261 cagccatttt ttgattgttt gctcaatctg acttttttga tttggctcac ttataagatc
     1321 aaaccactgt gcatccaaac ggttacgaaa ccacgtcaac tgacgtttcg cgtatcttct
     1381 cgaatgtagt ttgacttgtt ctgttgcttc ttccaatgtt tctttccctt caaaataagg
     1441 gaaaaattct ttatatccaa taccttgact ggcctggact tctccaagct catacacctt
     1501 acgcgcttct gctactaaac cttctgctag catcaaatcg actctttgat tgattcgttc
     1561 ataaagaata ctacgcttcg tatctaatcc aatcatgagg taatcataca attgctttgg
     1621 ctcttcttta ggtgccgtga tactatgtcc tgtcgtctct aaaacttcca gagctcttat
     1681 cactttgcgt tgattgttcc agtggatttt ctcagcagct gccgggtctt tttctttcaa
     1741 ctgattccat aatgcttggg aaccaattgt ttcagctaac gtttcatact tttttctaat
     1801 ctcaggattc gcttcttctg tcgctcctaa ctgataatca tatagaaggg attggatata
     1861 gagacccgta ccaccagcta tgataggtag gtgccctcgg ttcgcaatct cggtaatcaa
     1921 ttgtcgacct gaatgctgaa aatcaacggc tgagtaacgt tgagtaggtt cgatcacatc
     1981 aatcaaatga tgcggaacat tattcatctc ttcttctttg atttttgctg taccgatatc
     2041 cagattttta tagatttgca atgaatcacc actgatgatc tctcctccaa atagttgtgc
     2101 gagttccaca cttagcgctg ttttaccgac tgctgtcgga ccgacgatca ctaatacttt
     2161 ttccatattt tacctacttt tcgttgattg tttccaccga agagctttct ccggaaaatc
     2221 agttattatt ccatctattt tgagttcttg acatttggcc atttcttctt catcgttaat
     2281 cgtccatgca cgaattgctt ttggaaaatc agctgcctgg actggatgtt cgaacaacca
     2341 agtatttttt ggatgtactc cttctatata aggggtttct gctgctagtt ggatcttttt
     2401 atcagaagta ctcatcaata agtccagctg tatctccggt tccagtaatg cgattttttc
     2461 caaagaatcc aaatgaaagc tgcaatatgc atgttgaaaa ggccaatctc tactttttaa
     2521 tgtttcggct aatttttctt cgataccggg atattcatac tgatcagttt taatttcaat
     2581 cattagcatc cctcggtaat ttttttggag gagcagtcct aacacttctc tcaattcagg
     2641 aactttagct gctggaaatt ctttgctgaa tttgccccct gcattcaatt ttttgagttc
     2701 ttccaatgtt ttttctcgaa tcagcccttt accgttagtc gtccggtcca cttcttcgtc
     2761 gtgcataacg atcaaataac catctttgct tagatggaca tctagttcga taccatctgc
     2821 acctactcgt acagcttctg caaatgcagg aagcgtattt tctgggtggg tgccgctgct
     2881 tccgcgatgg gcatagattt gtaacatctt tcttcactcc gttcagttct attgtatcat
     2941 taagaaaaaa agaaagcatt accacgctct catgtgcaac ggcaatgctt caaaatatca
     3001 gctccaaaca tcatctttcg agaagaaacc aatcggctgg cgcgtcatag tcttcaactg
     3061 agcttttttt tcattgatca tttcagtcag actatccgta aaattctcta aaatcacgcg
     3121 tccgccttta taagcatatc caccaatctt cattgtttca tctggagata aaatcacttt
     3181 tcgatgggag atattcttaa atagctcgtc taaaatataa tttggcagat aaaaactatt
     3241 ttgcgctgaa aatgattgga gatacgcaaa atcgtcaatc gtgatataac aatcatcaaa
     3301 tgcatcaaag ctgacatatt cttccatcag cgttgtccgt ttttgcaaga tctcgttgat
     3361 ctttttattt aattgattca gcgtctcgat ttctttgact ttcaattcca attcacgtct
     3421 acgctgttct ttattttcca agtgacgcat ttgcagtatc atgccgatat agacaactac
     3481 agcacctgtc agcactccga taaggccagt aataccaact atcaatctgt tgttcctcct
     3541 tgttttgttt ctctgcttat cataccataa agcgggttta tgcgactaat ttgcctaagt
     3601 tttttatttg accttttttg accaaaaatg tatactacat tttaggtatt agaaactaaa
     3661 ttatggtaca ctatttctaa acgaaatcaa ggaggaactc tattatgaat ttaattccta
     3721 cagtcattga acaatcttca cgaggtgaaa gagcttacga tatctactca cgtttattga
     3781 aagaccgtat catcatgtta agcggccaag taacagatga tcttgctaat tcgatcatcg
     3841 cccaattgct tttcttagac gctcaagatt ctgaaaaaga tatctatctt tatatcaatt
     3901 ctcctggtgg ttcagttaca gcaggtatgg ctatttacga cacaatgaac tttgtcaaag
     3961 cagatgtcca aacaattgtt atgggtatgg ctgcttcaat gggaagcttc ttgctgacag
     4021 ctggtacaaa aggtaaacga tttgctttac caaatgctga gatcatgatc catcaacctc
     4081 ttggcggcgc tcaagggcaa gcaactgaaa tcgaaattgc ggcacgccat attttgcaaa
     4141 cacgcgaacg cttaaacaaa attttagctg aacgcactgg acaaccgctt gaagtgattg
     4201 aaaaagatac agatcgtgac aactatatga cagctgaaca agcaaaagca tatggattga
     4261 tcgatgaagt catggaaaac agttcttcat taaattaatc tgtttcaaag gaagcttttt
     4321 taagctctct tatctaaacc aaataaaaaa ggatggtcaa ctcaaactga ccatcctttt
     4381 ttattttatg gatgctactg cctatctttg ctccttgaat tttccttgcg catgcatccg
     4441 acctatctcg acataatgct ggatgttttc tttgtttttt tgtatttctt cttttgtcaa
     4501 aggacggatc actttagctg ggcgaccaaa agccaagaca ttaggaggaa taacttttcc
     4561 ttctgttact aatgatccag caccaatcaa actattctca ccaataaccg catgattcaa
     4621 tatcgtggtc cccatcccga tcagcgcccc tttttcgatt gtacagccat gtagcatgca
     4681 ctgatgtcct accgtcacat tctccgcaat atctacaggt gcatcatgat cgacatggat
     4741 gattgtacca tcttggatat tcgttctttg tcctattttt atccaattac tgtccccgcg
     4801 taaaacagct tgataccaga tcgtcacatc ttctgacaat gtcacatcac caatcactgt
     4861 ggcatttgat gctataaacc gttccatatt tatttttctc ctttcatttt ttgtagaaac
     4921 taatcctatt gaataaaggt atcatagcat atatttattt cataagaaaa aaaacaggta
     4981 aaacctgctc aggccagatg tctatttaca gtatctggta actgacgttt tacctgtttt
     5041 tattgtacat ctatattttc ttctattact ttttttaaaa ttaattcgta cacttctatt
     5101 tgtgctgcca agcgttcgat ttgaaactca ttttcaaaaa aatcagctga atcaaaaggc
     5161 ttcgtctgac agacgtgcgt taaacgatca acttctcttt tggcttcagt aattttttca
     5221 tttaaaaact gttccatctc cgtgtcctca cttttcttga taactttact ttaccattat
     5281 caagaataga gaacaaaaaa taagttttaa actgttttat tcttatatga cttattctgc
     5341 ttcctcagca aattggctgt tatacagttt ttcataaaat ccaccttgtg ccaataaact
     5401 ctcatgcgtt cctttttcaa tgatctgacc ttgatccatc actagaatca gatctgcatc
     5461 acggattgtc gataaacggt gagcgatgac gaaacttgtc cgtccctcca tcacgcgatc
     5521 cattgctttt tgaatcaacg cttctaaacg ggtgtctact gaacttgttg cttcatctaa
     5581 aatcaagatt ttaggatcgg agaccactgc tcgggcaatc gtcagcaact gtttttgtcc
     5641 taaagacaca ttgtctccct cagaattgat ggccatatca tatccatctg gcatcgttcg
     5701 gataaaatga tctacgtttg ctgttttcgc agcatcaact acttcataat ctgtcgcatc
     5761 aagctttccg aagcggatat tatctgcaat cgttccttga taaagccatg catcttgcaa
     5821 gaccatacca aatagcgaac gcacatcact gcgactcatt gatttggtgt cgattccatc
     5881 aatccttatt gctcctttgt ctacgtcata aaaacgcatc aatagattga tcaatgtcgt
     5941 cttgccggca ccggtcggac cgaccacagc tacggtttga ccagctttca cttcaaaatt
     6001 caaatcttgg attaatggtt tcttcggatc atatgcaaaa tcgacatgat cgaatgtcac
     6061 atctcctaaa atcgtttctg gcagtgaagc atctactttg ttttcttttt cttctggctc
     6121 atctaaaatc tcgaatacac gtttcgttgc cgctgatgca ctttgcaaaa ctgaagacaa
     6181 ttgtgtcacg ttccccattg gctgactgat ttgccaaata tattggatga atgcttgtag
     6241 ttgaccgaca acgatcgccc cagtaataac ataaaagctt cctagaacag ccataccaat
     6301 ataagtagca taggcagtta attgaactag aggcatcatc aagccagaaa tgaatgctgc
     6361 acgaaaccca aagtatttca agctgtgatt aacttttttg aatccttcca atgtttcttt
     6421 ttcgcgtcca tatactttca atacactaaa acctgtcata ttttcttgga cgtagccgtt
     6481 tagctcacca agagaattct gcatgctttg aaaatatttc tgcgaagcat tgatcactcg
     6541 ttttgagatc cagatcgatg ttggaatcat gatcatagag atcagcgcca tcatcggctg
     6601 gatataaaac atcattgctg cagccaacgt aattcccaat atcgcattta cgataccaat
     6661 aaaagcttgt tgtaaagcac cgctgactgc atctacatca ttcgtcacac gagacaaaat
     6721 atttccttgc tggtttttat cgaaatatga aaccggaaga cgattgattt tttcttctat
     6781 gtctcttctc aaatcatgca tggcactttg aacgacattt gtcatcaaaa caccagaaag
     6841 taattgcgaa atacaatacc ctattccaac aactgtgatc caaatgagac atttgaatat
     6901 ataagaaaaa ttgattgatt cgccattggc aatattacgg ctgatttctg tagtaggaag
     6961 tcctgtaaca tatggcaaag ccacgttgaa agccactgtc agaactgtaa ataggatcac
     7021 taaaagaaaa gtctcacgat acggtctgat atatcgagct aaacgtttta aggaagaaaa
     7081 ggtttttttc atgctaattc ctcctttgat aattgagaag cggcaatatc ataataaatc
     7141 gggcattttt ccaacaactc ccgatgcgtc cccataccga cgacttctcc ttcatttaat
     7201 acgatgatct tatctgcatg catgatcgtt cccacacgtt gtgctacgat caatacagta
     7261 gattctgtcg tttctttttt cagacgagca cgtaacttcg catccgtttg ataatctaat
     7321 gcagaaaagc tgtcatcaaa aatatagatt tccggacggc gtatgatggc tctggctatt
     7381 gccaatcgtt gtttttgtcc acctgaaaaa ttggaaccac cttctgataa atgttcatta
     7441 aatccatctg gttttcgaga aatgaattct gaagcttgag caatctcagc tgcacgttcc
     7501 atttcttctt gcgtagcatc ttctttcccg tatcgcaaat tatctgcaat cgttccagta
     7561 aatagcagcg ctttttgcgg aataaaccca atcttatctc gcaacttgct tagccgataa
     7621 tctctcacgt ctactccatc gatcaagatc tttccttcac tcacatcata aaagcgaggg
     7681 atcaattgga tcaacgtaga ttttccgcta ccggtacttc caataaaagc aacggtctct
     7741 cctggagagg ctgtaaaact gacatttcgt atcactggct cttgcgaatg gcctggataa
     7801 gcaaatgtta cattttgaaa ttcgatatac cctttagttt ttgtatcaga tataccattt
     7861 ggatcttcta caatcgaagg tttggcatct agggcttctt ggatacgtcc agctgaaaca
     7921 gctgctcttg gatacatcat gaacacacta gcaaataaca tgaatgagaa caaggcatgg
     7981 aatatatact cgataaacgc aatcaaatct cctacacgaa gctcgcctgc atcgatttgc
     8041 actgccccgc tccagatgat caataccatt acgatattaa ataagaaata aaaacccggt
     8101 tgtgcaacag ccatcaaacg gaataagctt tttgaactag aagcataatt ctcattgact
     8161 acagcaaaac gtttttcttc aaacttttca ttgacgaatg cacgaatcac acgcaatcct
     8221 gaaagatttt cccgcaagat actgttgatt ttatctaagt ttttttgttg ctttttagat
     8281 aacggttcag acaccttagc aatcgctacg actgccaata atagtaaagg taatgcacct
     8341 actacataca aactgagtgt cacacttgta cgcatgacca tgtacaaact gacaatgaac
     8401 atcattggtg ccataaaacc gatacgtaaa atattttgca ggaacaacat gatctgataa
     8461 gcatcgtttg ttgtacgagt gatcaatgaa gatacgccta gattttcgta ttcctgatgc
     8521 gaataacttt ggatcttttc gaacaaatca tctcgaatat ctgctacgat attagtcgta
     8581 atcctcgaac caaaataacc taacaagata ttcaaaataa taccgattac agtaataacg
     8641 atcataaata tcccttgttg cttcacataa tcaaaatcat cattgcggat tcctacatcg
     8701 atcatacgtg ctaaaatcgt aggaagtcct aattcaatca aaataaaacc aaatacgcat
     8761 atgaaattga aaaacaagaa tttcttataa cgcatggtat aatgccacat tagtttcact
     8821 ttctcatctc cttttaatct tttgttacat tttcattact aaaaat
//
LOCUS       DAZKNM010000110         8841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000110 DAZKNM010000000
VERSION     DAZKNM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.111"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..345
                     /locus_tag="ACG8M9_002024"
     CDS             151..345
                     /locus_tag="ACG8M9_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378753.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            342..836
                     /locus_tag="ACG8M9_002025"
     CDS             342..836
                     /locus_tag="ACG8M9_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378754.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(998..2170)
                     /locus_tag="ACG8M9_002026"
     CDS             complement(998..2170)
                     /locus_tag="ACG8M9_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0378755.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2376..3191)
                     /locus_tag="ACG8M9_002027"
     CDS             complement(2376..3191)
                     /locus_tag="ACG8M9_002027"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0378756.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3341..3787)
                     /locus_tag="ACG8M9_002028"
     CDS             complement(3341..3787)
                     /locus_tag="ACG8M9_002028"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0378757.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3784..4299)
                     /locus_tag="ACG8M9_002029"
     CDS             complement(3784..4299)
                     /locus_tag="ACG8M9_002029"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0378758.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(4372..5286)
                     /locus_tag="ACG8M9_002030"
                     /pseudo
     CDS             complement(4372..5286)
                     /locus_tag="ACG8M9_002030"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(5283..7610)
                     /locus_tag="ACG8M9_002031"
     CDS             complement(5283..7610)
                     /locus_tag="ACG8M9_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0378759.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            8219..8365
                     /locus_tag="ACG8M9_002032"
                     /pseudo
     CDS             8219..8365
                     /locus_tag="ACG8M9_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(8453..8746)
                     /locus_tag="ACG8M9_002033"
     CDS             complement(8453..8746)
                     /locus_tag="ACG8M9_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0378760.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         2473 a         1709 c         1616 g         3043 t
ORIGIN      
        1 atcaacacgg gattagatta agtttcctca atcactattc ataacacaat tattataaca
       61 agtaagtaaa caattgaaaa ggacatttat tgaaaacggt tgttttttac ttaaggagga
      121 aatttagcga aaaaattagt tgcattgttt ttggtcgcag gaagtttgtt gagtgggtgt
      181 ggtgctacta cgtctactac tcaaagcaca agttcagatc aggcttccag cagcagtacc
      241 acgatctccg aaagtacggc agattcatcg aaagaacaag aacaaattga aaagaaaaaa
      301 gaagagaaac aaaaagcgga aaaagctgca ctagaaaaaa agtgacagaa gcggaatctg
      361 ctttgtcaca agctgaagca aatcctacaa aagaaaacta tgcaactgct tcagcagcta
      421 tccaagcgct acctgaaaac aaacaagagt tgacaagccg tcttgctgca attgattcca
      481 caatcaaagc aaaagaagca gaagaagaac ggaaaaagca agaagaaatt gcacgacaac
      541 aagcagaaca agcgcaacaa gaagcaatcg cacagcaaca agcagaacag gcgaggcaag
      601 aagcaatcgc acagcaacaa gcagaacagg cgcgacagca atcagcagct gaacaggcct
      661 ctcagcaggc acaagccgca gctcctgagc aaaataatga acaaactgtc tatgtcacac
      721 cgaccggttc aaaataccat acacataaat gtggaaatgg tacgtatacc cctgccactt
      781 taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa atgttatgga aactaattca
      841 aaagttatac tataataata aaattcttct gaaacataaa aaaatagaag atggcgttcc
      901 agatagtaaa ccaaaaataa cagtttacac gtccatttcg tcagcttctc tcttttttca
      961 tttagtctgt tccctctgct tttttggaca tcttcattca ttctttctca atcaaatagt
     1021 acacctcttt ttcttttcgc agtttatatc ttcctttttt caatttcatt gaaaactgtt
     1081 tcgaatgttt atgttttaca tggattttaa gcaggagctc ctcatgttgc aagcgatagt
     1141 tcctgccttt taaatcagaa aatctcacta gtccgtattc atcagaagct ttcacaaatg
     1201 gaactttttc tctagtaata tagtgtttgc cgttgagcgt ttttacagaa aagaccattc
     1261 cttcgattgg tcgtccgttt gtgttcttta cataaaaaga taaaggatag tttcgcttag
     1321 cgatggccaa agaatctaaa taagcgatga gtagccaaac taagccgatt gatccaagta
     1381 gtataccgat aaaccaaata aaaaatttcc attttttcca ttgatcaatt ccttggatcg
     1441 ctttttttgc ctcttttgct tgataaggaa tacgatgccc cgtcacaagc agtcgatgtg
     1501 tattgaccat ataaggagta caggtaagca aagtcacgag atctctgcct ttttggatac
     1561 ccaaagaatc tacttcatca ggtaagacca cttggatttt ttctacttga tacgccaatg
     1621 tctttccatt gatctggatg aaaaactggt ctcctttttt caacttaggc aaatctgtaa
     1681 ataattttgc ttctgggagt ccacgatgcc cagaaagaac ggcatgggtg tttttcccgc
     1741 cagtcggata agaactttct tccaataatg acgttccttt ttgcaaaaat atttccgttg
     1801 tctgatcgaa tataggtaag ctgacattga tttttgggat ttcgatgact ccaagcatat
     1861 gttccataaa aaaggcatta ctaggtaaat cttttaatgc ttttgcatca actgcttggt
     1921 tgaaactgcc tatccctggt gatacgtttt gctctgccaa ctgttggttt ttctttgtca
     1981 ttttttcctg ctgttttttt atttcctctg aattcttatt cgatgcttgc ttttgataat
     2041 ggatgatcat ttgctgggca agaaaatcat tcagtgaatc ttctacaaaa ggataagaaa
     2101 agatcccgat cccaacgact aaaatgattg ccatgaagat gtccagtatt cttattctgc
     2161 ttttattcat catgtccacc tattttctaa tatgtatatg aagaggttgt gaaatcagcg
     2221 ccttgacctg agtattagcg aagcaaacgg caaaatagtt cctcctattt tgccgtttgt
     2281 tgagcgaata ccaagagacc gaccacttgt cacaaccttt ctccccattt gaatcactat
     2341 cgtttattta tcgtatgatt tttgtttcca atagtttaag cttctgtttc ttgatggcgt
     2401 tttttgaaat agatcactgc tcctccaact aaagcagtac ctaccaagat gaatacgatg
     2461 atccccatac caccagttga aggcaagatt cctttaggtt ggttatcgat cgttgtttca
     2521 tcagaagttg ttggatcatc atctactgta aattcagtgt acgcagcttc attcttaggt
     2581 aatgcgtagc cttctggtgc ttttgtttct tgaaggcagt atgccccttg tttcagccct
     2641 tcgattgaaa atttcccttc tgaatcagaa atcaatttca cttcttccca gtcagaacct
     2701 gtgacttcat ctgaccaagc atatgggtat tctcctgagg caaggtttgc tatttttttc
     2761 attaatttaa cagttggatc ttttgctctt agaatagaaa attctgcatc ggctaatttt
     2821 tcttgcgtgt tcacatcttg tttgatgaag tggaaatcac cgtagttttg gacattttct
     2881 acagtaatcg tgttatccgt agttgtattt tcccgattga ctacaccgtt tggattccca
     2941 agattggctt catctgcgcc atggatgata ccggttgttt gttgcaattt tccgtttttt
     3001 actataaaag tagtttcttt ttgtggattg gttaattgat aaccatctgg tgcttttgtt
     3061 tcaactaaag tataattgcc gtctattaat ccattgacac tgatggatcc ttcagtatca
     3121 gttgtgaatg tcaacgctga ctcagcagga tcatagagag cgacaccagt gttttcatca
     3181 aaaccggtca cgaattgatt ctctgaattt ttgatctgga attgcgcccc tgctaactta
     3241 tctacccctg aaaagttccc agctttctgg attttgatat tgctttcttt gatgacattt
     3301 ttagcatata ggtgaatcgt gtctaaaggt ttgtcagggt ctagggtaag cttaacacga
     3361 tatttggcga cgtttcgaca ccgggctttg gtgtttctac aaagacgtat acagcatctt
     3421 tcccatcaga tttacctgga agagtcaggt tagctaatcc ttcaccagaa gtcgttacac
     3481 ttgcgaaggg tgtagcgtct gctggaactg ctgtgtcttt acttggatag tacgtagcag
     3541 tgatgtcata tacgttgaat tcaacgccgt tcaacggttc gctattcgga aatgtcaatt
     3601 ccgttccgtt gtttatttga ttttctggtg ctgaagtgaa tttcaattta tgcaaattaa
     3661 ccgtaacatt ttctggtgta ggtgatacgg ctgcagaaac ctctgcagtt cctaggccga
     3721 gaacgaaagg aagcagcatg atagcagttc ctaaaaacgt ccatattttt gttttgatgt
     3781 ttttcatttt atttccctcc tattgtttcc tttttgttgt tttttataaa accaagctgt
     3841 ccctgttgaa aggactagca ataaccctaa aattgagaaa cctccaagca tctcttcgct
     3901 tgtttttgga aagcttcctc catttttttc atttgatgga ccatttgtct gcatctgaac
     3961 tggcgttttt ccattattcg tcggatttgc ttttggttcg ttgatgattt ccaatggtat
     4021 aggatgaagc ggatcataac tgcctttttc aacttcaaag agaatggttg attgactcaa
     4081 tatatatcct ttaggcggtt taatctccac tagttcgtaa cttccaaaag acaatccatg
     4141 gacagaaaat gttccatctt tactggaagt aaaaatcgtt gctcttgaat catctttggc
     4201 tacccaatga tttatcccgt tttcttgcgc taagtattgg tcattttttt tgactagaaa
     4261 ttgagcatct gctagttttt gtttcgtttg ccgatccact ttgacaaatt gtttgcctcc
     4321 tgtttggatg gcaagatgtg tttctactgt ttgattgtca gtcgtcaaac gtacatcgtt
     4381 atcaaatgtc gttttttcac tgttttcttt caatgtcatt tggtaacgga tcgttaattt
     4441 ttttccagca aacgaactaa gatcttttcc agaatctgat aagacaaaac catgttttat
     4501 tttttgtgtg tgaaatgtat gaatcttgtc tccaccaatc aaaaaatcga tcgtttctgg
     4561 ttctagccat aactgaggat cagctgtatc tttgatcgta taggttccta tctcgttgat
     4621 gttctcagga attgttacgg tgatgagata agggacttta tccccatctg caaaatctgt
     4681 ctgcttattc tctactgttt ttgttaatgt gagtgcagca ggtatttttt gttcgctctt
     4741 tggatacaag tggatggtcg tcaatttatg attagatgaa tctgtcacag gaagcacaac
     4801 gacaaatggt gctgattttt cttttactta atctgaagtc tttatttccg taaatcggta
     4861 aacagcgtca cgaccttgct catctttgtc cgatgcacta aaagaagcaa tgccctcttc
     4921 ctcgtctact gtttttgtca cattttctgc cagtattttt tcactatcac ttttttgtgc
     4981 tagttttctt tgtgcgtctt ccacactact tccttctgac cgcagcttgt aaaattcatc
     5041 cgagacatca tatatagcga aagtaacgcc gttcaaccct tttactcctt gaaagttatc
     5101 cgggatattt attccgtcgt tttgactgat acttggcatc ttatcgcttt cgaacattcg
     5161 attatgcaaa gtaaatgtaa taggtgattc tctagctgac acagtcggag aacctataaa
     5221 aaagaccagt aggataaaaa agagaacagc aaatatatcc acacgtacat tttttgtttt
     5281 tttcatcgct gttcacctgc ttttttctta cgatggataa aataaaagaa gccagaaaaa
     5341 ataaagatca gtcctaaaaa gacaaagggc agtagaccta tcccacctgt cgaaggaagc
     5401 aagagagtcg gattgatcgt caacttagcg gtatctgttg cgataatcgt ataattattg
     5461 tttacacttc cttcgcctaa actaatcgtt actatttgtg atccaatatc tgtccggctg
     5521 ttaactgtta gggtataatc caatgattcg cctaagacaa caccggtaac gtggagattt
     5581 ccaaccgaaa tcgagtgact ttgggcatca taattaaagg cagcgtcatc gacagtaatc
     5641 actgcgttag ctttatagat ggtataagtt cctgatgtgt tggctatatc agtaaatgac
     5701 aacttgggat ttgctgcttt gactttgtct attccgctat tcgttagttt aattgtatag
     5761 ctgccagcat taaccatatt ttttgctgat tgtgcatctg ctgtgtaatc catactgcta
     5821 agtgccactt gtactggagt aagcgaacta gtgacaccct ctgctagctt gacatggatt
     5881 gacgggacac ctgcttttcc atcatatgtc ttactgccac tgagctctac cgcaccgctg
     5941 atttgcttaa tcgtatagac atcagatgtc tgcacgatag taaataaata gtttgttctg
     6001 gcagctagtt tagctagtcc gctttttgtc aattgccaag acgtataggt tccaatttca
     6061 gtaggcgcag ttacttcaac tcccttttca ttcatcgctt tgaaatcagt tggattccat
     6121 gtgatttgat caatactgag atcattgttt aaaagggatg ttgattctgt tttatcatta
     6181 aaggcatatt taacgatcgg tacaacagaa agaccatcat agtacttact gccatttgca
     6241 ttgattgata ctgttgcatt tgtcttataa tataggatga cttcagaaat ctgttttcca
     6301 tttgcgtcct ttacgattac atttccattt gtatctaaac tagctaaaac actcattgct
     6361 gattggttct ttgtttctat cgctgaaaca gctgtaaagg tatatccgtt gatagtaggc
     6421 gtattagtcg tcaggtcttg tgcagcactt ccgtaagtaa gattcattat tttagaagga
     6481 gcaatcatct gtccaaaata gtcttgatac ttaatagtga ttggtgtttg tttgcctgta
     6541 taggtcaccg tatcgacgtt actttctagg gctgtgaaag aaccgtttgt ttgtgcaaaa
     6601 gaatagcccg ttactgctgt tggagtgata accactttgt cacccacatg gattccagat
     6661 aatgtagtcc cgtacgttcc gatttttgct gctccaccat tgactgtata agaaaaagtt
     6721 cctgtccgat tcgcattgac ctgaaccgtt gctgcttgat aggttggaaa atttgaaaca
     6781 tttcccttac catcaacgat ctgcccattt ttgatcgagt accccgtgaa tggaatatca
     6841 tttgctaact gtataccgat tgaaaaagta ctcatacctc ctccggcagg aagccctccc
     6901 catgttgagg gtgtaaaaga ataactttta tccgcattgg gtgtaactga atcgatcgta
     6961 tttccagcgg tagccacccc catatagata ctttttggga atgtgatagt agtaccattt
     7021 actgtggagc caataaacag attatggata tctggacggt tatcaataaa agcgattgct
     7081 ttggttacgc cttgtaacgc agttttatcc gcactcatcg atccgttaac cgcaacaatg
     7141 actccatctt tggcatatat cactgtatat ccatctggta tggtatgtcc gttgaaattc
     7201 gcagtggccg caacagtact aaatgtctgg cttctacctg tttctgaata agctttctcc
     7261 cctgctgctt ttgctgctgt gatttctttt tctgtcggat caatcagttg agtcgaggat
     7321 gtggttgagg ttgtctcgtt gttgctagac tgattggtgc tttctatgga ctgtatttca
     7381 gaatagggtg gactatttgt ctcctctgtc gtttggttag caaaatttga cgtttctgta
     7441 taaacgttta cataaatact tgataagctc cctaagaaag taaaaatcaa gatgatcgaa
     7501 atcataatgg gatgtaagta ttttctttta gatacagctt tgagtctaga aacaaaatgt
     7561 ttttgacata tacgttggat tttattttga gttttcttaa tcattttcat ccctcctctc
     7621 aataaaaaat catcaaataa tcctttacac gttattctaa atttaatttt tattatttta
     7681 ttgatgtata gaaagtttgg ggcttttttt ctctttttat tctaaggtta ttttagtctt
     7741 gtcctttaat acaaagattt tatttttaaa taaaaaatac tcttttttta cgattattga
     7801 aaaaaaaaga taaaacttga ttaattgaag tattgcttaa atttggaagc tatgagggaa
     7861 gaataaaaga gttaggacgg gattatctac tatttttctt tgttttaaat atttgttatc
     7921 tttgaacata aaagagaaat gtgtcgactg cagtgttgtg tccctcatca cgaataaacg
     7981 gtgttcaact agctgatctt gtattcactt aaaaaatggt aaagtacgag ttgctattcc
     8041 ccattttcaa ttttctaacg ctcaggacaa aatactgttt tcacaacctc attggaataa
     8101 aagcatatat attgtttcaa gaacgaaatc ttgttatctt tttaaagggc atctgtccaa
     8161 aatctaattt taggaggtac gttttggtat ttattcgtac aaataaaaga gaattatctt
     8221 tattcatgta acgcatccaa ttgatttttt catgtacgct cctggaacga ttttactgac
     8281 gattgcttat ttaactgcga atcgctcatt agcttttatc atagctatcc atattttgaa
     8341 taacgttctt ggctttgtat tatagataca gatgacaaaa aaagttcaag tatgcggtgt
     8401 agaaccaata tcgcatactt gaactttttt caatgagtat aaattaaaac ttctaatttt
     8461 caattcccat catctgatat gcccgaccgt ctgaacttct tttgaaaaag ccaaatgtga
     8521 ttagataacg tctttgttcg acataatcgc cactagaaaa tacgttcagc agtatcgaat
     8581 taacctctgt ttctgtataa gtcactccat ggtcaaagcg tttcaagact tctttcaata
     8641 ggatgtattt cttttttgtt gaacttggta cttgttttac ttttccatca ttcatgaatg
     8701 tgtctaaaac tttcatcttt tcttgctccg tataagtata aaccatcttc tttctccttt
     8761 tgattctaag aatactccat ttatgttaga atattctgga ttttttatta aagcggttac
     8821 tcttaagaaa gatattatca t
//
LOCUS       DAZKNM010000111         8840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000111 DAZKNM010000000
VERSION     DAZKNM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.112"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(392..889)
                     /locus_tag="ACG8M9_002034"
     CDS             complement(392..889)
                     /locus_tag="ACG8M9_002034"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0378761.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(929..1738)
                     /locus_tag="ACG8M9_002035"
     CDS             complement(929..1738)
                     /locus_tag="ACG8M9_002035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0378762.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2072..2929
                     /locus_tag="ACG8M9_002036"
     CDS             2072..2929
                     /locus_tag="ACG8M9_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0378763.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2922..3824
                     /locus_tag="ACG8M9_002037"
     CDS             2922..3824
                     /locus_tag="ACG8M9_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0378764.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3857..4207
                     /locus_tag="ACG8M9_002038"
     CDS             3857..4207
                     /locus_tag="ACG8M9_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0378765.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4256..5179
                     /locus_tag="ACG8M9_002039"
     CDS             4256..5179
                     /locus_tag="ACG8M9_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0378766.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5257..6150
                     /locus_tag="ACG8M9_002040"
     CDS             5257..6150
                     /locus_tag="ACG8M9_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0378767.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6188..6751
                     /gene="amaP"
                     /locus_tag="ACG8M9_002041"
     CDS             6188..6751
                     /gene="amaP"
                     /locus_tag="ACG8M9_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0378768.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6765..6953
                     /locus_tag="ACG8M9_002042"
     CDS             6765..6953
                     /locus_tag="ACG8M9_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0378769.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6966..7868
                     /locus_tag="ACG8M9_002043"
     CDS             6966..7868
                     /locus_tag="ACG8M9_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0378770.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7900..8142
                     /locus_tag="ACG8M9_002044"
     CDS             7900..8142
                     /locus_tag="ACG8M9_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0378771.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8398..8769)
                     /locus_tag="ACG8M9_002045"
     CDS             complement(8398..8769)
                     /locus_tag="ACG8M9_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378772.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3078 a         1531 c         1718 g         2513 t
ORIGIN      
        1 aggtacataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
       61 aaacagcttc ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat
      121 accgtttaag gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg
      181 gaaaaatagt tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga
      241 acaccattta agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa
      301 tctactttga tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg
      361 tgttaaatat cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat
      421 ctttcaacag ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa
      481 caaaaaagtc tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca
      541 agtttgtttc aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag
      601 tttcctggat ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc
      661 gttcattttc ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt
      721 tgtccttcag ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg
      781 ctttagctaa tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt
      841 cgctgtttgg atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc
      901 ctacattcat atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac
      961 cagcttcttc attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat
     1021 tccccatcgc aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc
     1081 caaaagccat gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat
     1141 gagctttagt gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt
     1201 ggataccatc tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg
     1261 ttaatgctaa tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa
     1321 aagaatggtt ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt
     1381 catcgaatgt cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt
     1441 cttgagcata gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa
     1501 ctttcgccat ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat
     1561 agccaccgat tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag
     1621 cgacggttac gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg
     1681 gtatctttcc atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt
     1741 tatcttccta ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat
     1801 ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag
     1861 aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa
     1921 acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga
     1981 aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt
     2041 tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt
     2101 ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt
     2161 agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga
     2221 atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca
     2281 attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac
     2341 taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt
     2401 cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc
     2461 gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag
     2521 attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga
     2581 tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga
     2641 tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca
     2701 acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa
     2761 tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac
     2821 aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa
     2881 aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag
     2941 cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag
     3001 tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg
     3061 attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg
     3121 tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc
     3181 cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac
     3241 gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc
     3301 ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg
     3361 aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca
     3421 atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata
     3481 atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt
     3541 ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac
     3601 atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc
     3661 atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt
     3721 acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa
     3781 aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag acaacaactg
     3841 aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga
     3901 aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct
     3961 aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga
     4021 atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt
     4081 agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt
     4141 ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt
     4201 cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga
     4261 tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag
     4321 tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt
     4381 cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact
     4441 agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa
     4501 aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag
     4561 aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga
     4621 aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga
     4681 gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga
     4741 actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga
     4801 tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga
     4861 tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat
     4921 ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct
     4981 aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt
     5041 cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt
     5101 tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg
     5161 gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt
     5221 tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac
     5281 ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta
     5341 cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa
     5401 ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg
     5461 attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa
     5521 gatgcagagg aagtagcaaa atacgtgaaa gttgcaggac gtaagattgt cttactacct
     5581 tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt
     5641 ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct
     5701 gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct
     5761 ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct
     5821 gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc
     5881 gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt
     5941 gtagcacctg gtccaatctg gacaccatta caactagacg aaggacaacc ttctgattcg
     6001 atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca
     6061 ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga
     6121 gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg
     6181 tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc
     6241 tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa
     6301 gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt
     6361 tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat
     6421 cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga
     6481 aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt
     6541 caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc
     6601 actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt
     6661 gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa
     6721 acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt
     6781 cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga
     6841 tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta
     6901 tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa
     6961 catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt
     7021 ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca
     7081 aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag
     7141 aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta
     7201 aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc
     7261 cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa
     7321 aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa
     7381 tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg
     7441 aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac
     7501 ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag
     7561 cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa
     7621 tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca
     7681 acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc
     7741 aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata
     7801 aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg
     7861 ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt
     7921 atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg
     7981 gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta
     8041 ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc
     8101 attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag
     8161 gtcgtgaaaa agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca
     8221 tatttttcat ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg
     8281 gaataagagg ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca
     8341 attagatttt taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta
     8401 ataaaacaat gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac
     8461 gaccactttg aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa
     8521 gcaatggatc agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt
     8581 tactcttgtc tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag
     8641 taaacaaagt ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag
     8701 caggaaaaat gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat
     8761 aaatggcata ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt
     8821 tgtttgtgaa gctttttcgt
//
LOCUS       DAZKNM010000112         8794 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000112 DAZKNM010000000
VERSION     DAZKNM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.113"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..299
                     /locus_tag="ACG8M9_002046"
     CDS             <1..299
                     /locus_tag="ACG8M9_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378773.1"
                     /translation="DYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPV
                     RTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(396..830)
                     /locus_tag="ACG8M9_002047"
     CDS             complement(396..830)
                     /locus_tag="ACG8M9_002047"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378774.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1062..1772)
                     /locus_tag="ACG8M9_002048"
     CDS             complement(1062..1772)
                     /locus_tag="ACG8M9_002048"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0378775.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1762..2622)
                     /locus_tag="ACG8M9_002049"
     CDS             complement(1762..2622)
                     /locus_tag="ACG8M9_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0378776.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2627..3265)
                     /locus_tag="ACG8M9_002050"
     CDS             complement(2627..3265)
                     /locus_tag="ACG8M9_002050"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0378777.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3280..3579)
                     /locus_tag="ACG8M9_002051"
     CDS             complement(3280..3579)
                     /locus_tag="ACG8M9_002051"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0378778.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3622..5106)
                     /locus_tag="ACG8M9_002052"
     CDS             complement(3622..5106)
                     /locus_tag="ACG8M9_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0378779.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5126..5587)
                     /locus_tag="ACG8M9_002053"
     CDS             complement(5126..5587)
                     /locus_tag="ACG8M9_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0378780.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="ACG8M9_002054"
     CDS             complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="ACG8M9_002054"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0378781.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6890..7660
                     /locus_tag="ACG8M9_002055"
     CDS             6890..7660
                     /locus_tag="ACG8M9_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0378782.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7933..8760)
                     /locus_tag="ACG8M9_002056"
     CDS             complement(7933..8760)
                     /locus_tag="ACG8M9_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378783.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2656 a         1856 c         1383 g         2899 t
ORIGIN      
        1 tggattatac tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt
       61 tcatgtataa agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc
      121 gtataaagcc agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta
      181 ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata
      241 ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc
      301 tgagcattgg aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact
      361 aagctagaat tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc
      421 ttttttcttc tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat
      481 cctactgatt cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg
      541 actggtccat aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct
      601 tggcgctgat acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca
      661 gtgatccaac gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca
      721 gattgttcaa atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg
      781 ctcttatata attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc
      841 ctttcttata caattctccc tctaataata cagttctttg actatttaat ctatcacata
      901 taactctcct gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat
      961 actttttgat aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat
     1021 caagtgttat cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca
     1081 tactttccat gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata
     1141 ttggggttga tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg
     1201 ttttctactg ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt
     1261 actccaggga cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc
     1321 cctgtatgaa cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga
     1381 accttaccat aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct
     1441 tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa
     1501 gtttgataca aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct
     1561 attatatttc ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg
     1621 acaccgcttg gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc
     1681 caagtcaatt tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc
     1741 ttcattgctg caattttttc ttcacaagac atcatagcca gcctccttca atttagaact
     1801 taagtagtct tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat
     1861 ttcaatcaga aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa
     1921 atcaacacaa ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac
     1981 agggtaagta tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg
     2041 agccggatta ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa
     2101 aggtgaatga atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc
     2161 atccatgatt tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt
     2221 tattccttcg ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta
     2281 tccagctatt tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat
     2341 tagttgtttc gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg
     2401 cgcacttaaa caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc
     2461 agctatcttt tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga
     2521 catctcgata aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat
     2581 atctttgggt aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac
     2641 gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac
     2701 cacgaccagt gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat
     2761 ttaatccacc agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata
     2821 aatctttttc accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta
     2881 cctgccggat accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga
     2941 gttctacttg caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac
     3001 aacaaatgac tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc
     3061 cagcgtctgc acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac
     3121 agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta
     3181 ctacatcacc aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg
     3241 caatttgcaa attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca
     3301 ctttgtttca gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat
     3361 aactgtaact ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa
     3421 aactacatcg tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt
     3481 ggtaattggt acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct
     3541 tgttcctgat ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt
     3601 gttgtctaat cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc
     3661 tacaatcaaa aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa
     3721 accaatgaca actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa
     3781 tggccaagct gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat
     3841 ccatgtagcg aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag
     3901 gcaagcggct ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa
     3961 gaacaacgga atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc
     4021 aatcatcaaa aactgtccca aagcaccaaa caagacccca atcgttaccg catttggcga
     4081 accaaagcca aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt
     4141 tgagattcct tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa
     4201 gatcgccaga tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg
     4261 cccttcttgc atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat
     4321 cccaaagaaa aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa
     4381 gaatcctggt aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt
     4441 ttcatcccgc tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg
     4501 ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa
     4561 gttagaagct actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat
     4621 cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac
     4681 ctgaacgttt ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa
     4741 taagatattt aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc
     4801 aaatgtctcc attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat
     4861 ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa
     4921 accaccagag ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat
     4981 gcattcatga agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag
     5041 gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc
     5101 ccccatcttt tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat
     5161 cgatggagtt cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata
     5221 atttttggat attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac
     5281 tagccgtttt ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg
     5341 taaatgaaat agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta
     5401 cttgaggtgc gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca
     5461 catacgtttc atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt
     5521 cacgccaatc cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt
     5581 tcagcaagaa actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat
     5641 cgagaagaca tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg
     5701 atagatgaat ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc
     5761 ctttttgaat ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat
     5821 atcgtagtgg acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc
     5881 aacagaagta actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac
     5941 atcgattttg tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc
     6001 atgataaaga ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc
     6061 ctgaggcatt tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg
     6121 atcaaacgct tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt
     6181 tactgtaatc gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt
     6241 tggcccaatg aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc
     6301 tagatgatcg gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg
     6361 atcgattacg aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg
     6421 catcatctga tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca
     6481 ccatagatca cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc
     6541 cagccaccac atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa
     6601 gtatgtgccc cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac
     6661 agttttactc atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa
     6721 tgtgtatcag catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct
     6781 ttcagtaatc aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa
     6841 ctagtcaaat aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc
     6901 acttgtcaat attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca
     6961 actatcaact ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga
     7021 tttaaattta ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa
     7081 ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc
     7141 cagagaagct agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac
     7201 tgcgttgaaa acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt
     7261 gaaaatcaat tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat
     7321 gcgctttcca aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa
     7381 tgcagacatt tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa
     7441 gatattaaat gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat
     7501 tttagtcgct gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc
     7561 agtctttgat tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag
     7621 aaatacgggg ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt
     7681 aaataggatt atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc
     7741 tcaaattgat aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg
     7801 cttcttttat aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag
     7861 gtatgacaaa agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat
     7921 ttcacaacca ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt
     7981 gaaacgcgct attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg
     8041 cttatggcaa gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt
     8101 cttattatac tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt
     8161 acggtaatag gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt
     8221 ctcactcaat attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt
     8281 tgacaagtag atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt
     8341 cgtttgttcc aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag
     8401 atgatcaagc agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt
     8461 ttcctgatcg atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa
     8521 tgattgaatc tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc
     8581 ggagatctcg acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat
     8641 attcttgttt tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac
     8701 ttgtgttaac ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa
     8761 aacaatttcc ttccctttaa aaaattattt ctcc
//
LOCUS       DAZKNM010000113         8787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000113 DAZKNM010000000
VERSION     DAZKNM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.114"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8M9_002057"
     CDS             291..1430
                     /locus_tag="ACG8M9_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0378784.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8M9_002058"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8M9_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0378785.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8M9_002059"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8M9_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0378786.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8M9_002060"
     CDS             3334..4302
                     /locus_tag="ACG8M9_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0378787.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8M9_002061"
     CDS             4415..4693
                     /locus_tag="ACG8M9_002061"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378788.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8M9_002062"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8M9_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0378789.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8M9_002063"
     CDS             5150..5905
                     /locus_tag="ACG8M9_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0378790.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8M9_002064"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8M9_002064"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0378791.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8M9_002065"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8M9_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0378792.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
BASE COUNT         2952 a         1579 c         1903 g         2353 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgc
//
LOCUS       DAZKNM010000114         8715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000114 DAZKNM010000000
VERSION     DAZKNM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.115"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1154
                     /gene="mvk"
                     /locus_tag="ACG8M9_002066"
     CDS             210..1154
                     /gene="mvk"
                     /locus_tag="ACG8M9_002066"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0378793.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1147..2124
                     /gene="mvaD"
                     /locus_tag="ACG8M9_002067"
     CDS             1147..2124
                     /gene="mvaD"
                     /locus_tag="ACG8M9_002067"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0378794.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2137..3222
                     /locus_tag="ACG8M9_002068"
     CDS             2137..3222
                     /locus_tag="ACG8M9_002068"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0378795.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3219..4283
                     /gene="fni"
                     /locus_tag="ACG8M9_002069"
     CDS             3219..4283
                     /gene="fni"
                     /locus_tag="ACG8M9_002069"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0378796.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4532..5335)
                     /locus_tag="ACG8M9_002070"
     CDS             complement(4532..5335)
                     /locus_tag="ACG8M9_002070"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0378797.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5355..6197)
                     /gene="kduI"
                     /locus_tag="ACG8M9_002071"
     CDS             complement(5355..6197)
                     /gene="kduI"
                     /locus_tag="ACG8M9_002071"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0378798.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6213..7220)
                     /locus_tag="ACG8M9_002072"
     CDS             complement(6213..7220)
                     /locus_tag="ACG8M9_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0378799.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7227..7871)
                     /locus_tag="ACG8M9_002073"
     CDS             complement(7227..7871)
                     /locus_tag="ACG8M9_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0378800.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7971..>8715)
                     /locus_tag="ACG8M9_002074"
     CDS             complement(7971..>8715)
                     /locus_tag="ACG8M9_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0378801.1"
                     /translation="PYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVLKILDT
                     LLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETIHLGVL
                     DNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLEKHPLI
                     PYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAFSVSMP
                     KYRLTDETKEQIISALKQTKSEIEQRIQK"
BASE COUNT         2481 a         1863 c         1734 g         2637 t
ORIGIN      
        1 gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa cgtattctta
       61 gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga gacccttagt
      121 ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt ttgcttaaaa
      181 atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga gaatcaagcg
      241 gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg attgcctttc
      301 ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg gacgatcaac
      361 tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta aaaaatatca
      421 aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg caactgacca
      481 ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc gccacagcag
      541 tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata ctattagaaa
      601 atgtccagct ttcggaaaaa atcgcccatg gtaatcctag tggaatcgat gcagccgcta
      661 ctagcagctt gcagccgatt tattttacaa aagggcatcc tttcgactac ttttctttga
      721 acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca agagaagccg
      781 tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa atgatccaaa
      841 aattaggata cttgacgaag caagcaaaac aagcaatcat tgaaaattca ccagaaacgt
      901 tggcacagac aatggatgaa tcacaatcac ttctggaaaa gctgacaatc agcaatgatt
      961 ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc aaattaactg
     1021 gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa gcccaagaaa
     1081 tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga ttaggagtac
     1141 atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat caaatattgg
     1201 ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt aacattggat
     1261 gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga tgaattttat
     1321 ctagatggca ccttgcaaga cgaaaaagca acaaaaaaag tcagtcagtt tcttgacctt
     1381 tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa tttcgttcct
     1441 accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg cgcttgcaat
     1501 actgctctta agcttggatt agatgatctc tctctttcaa gatttgctcg acgcgggtct
     1561 ggttcagctt gccgaagtat tttcggtggt ttcgtcgaat gggaaaaagg ccatgacgac
     1621 ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt agcaatggtt
     1681 ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat gcgtcggaca
     1741 gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga tctatatcaa
     1801 ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat ggaaagaaac
     1861 ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta ctggtctcct
     1921 gacagcttaa aagcgatgga tgcggtgcga caattgagaa agcaaggcat tccatgctac
     1981 tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca tttatctgag
     2041 gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc acatgctggt
     2101 ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc tgcaccaggc
     2161 aaactctata ttgccggaga atatgcagtt gttgaaacag gccatccagc agttatcgct
     2221 gcagtcgatc aattcgtgac agtcactgta gaatctgcac gaaaagtcgg aagtatccaa
     2281 tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga attagtttta
     2341 gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgcttgac tgaaaagtat
     2401 gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag tgagttagac
     2461 agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt tgcaacggtc
     2521 aaagccttga atgtttttta cgcgttgaat ttatctcagt tggagatttt caagattgca
     2581 gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc tgctagctgt
     2641 tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga acaagaaact
     2701 cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat tgagccattg
     2761 attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc ctctacttct
     2821 gatttggtcg atcaagttca ccgttcgaga gaagataaaa tggtggctta tcagcttttc
     2881 ttaaaaaata gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga aaataatgta
     2941 acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt atctgcaatc
     3001 actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc tgaacagtat
     3061 gaaggagccg caaaatcttc tggtgcaggt gggggcgatt gcggaatcgt aattgttgac
     3121 cagaaatctg gcattcttcc tttaatgagt gcatgggaaa aagcagaaat cactccactg
     3181 ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa gacgaacatg
     3241 tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc gtacggatca
     3301 tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca caggcagttg
     3361 ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc gaaaaaacaa
     3421 aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg attgccactg
     3481 gttcagtcag cgctgccttg aaagtccctt ctttagccga tacttatacg attatgcgac
     3541 aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct gtcgaaagag
     3601 ccaaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg aatgcacctc
     3661 aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta cttattcaag
     3721 aaactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt ggcatgacaa
     3781 gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc agcggtcgaa
     3841 gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa ctgagttatt
     3901 tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa gcagacactt
     3961 ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt aaagcccttt
     4021 gtctcggcgc aaatgccgtg ggtacttcag gaaccgtact gacgcatctg atgaatcacg
     4081 gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt ctgctttata
     4141 ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atctctgatc ttttccggac
     4201 cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga agacgcacag
     4261 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtacaaac
     4321 ggcggaagta aaagcaactc cttcggaaat aagctaaaat tcacaaaaat ttgaaaagca
     4381 attttcgtga attccttctt atttcttgga gttaaatgct tctgtcccag cctctttttt
     4441 cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaatccgct gatttcacaa
     4501 cctctccact aaacagtgta ctaaagctct tttaaggttg ttttccgata taggcaagta
     4561 tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta gaagccaaga
     4621 agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc gtacgtccaa
     4681 cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt aatggagcag
     4741 tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg tattgaccgt
     4801 attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca gcataagcac
     4861 tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt ttaccgccgc
     4921 ctttttcgat catatccgga ataactgctt ttgaaacgat gaatggggca tttaaatcca
     4981 cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc cgtttgatga
     5041 tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc tgttgaacca
     5101 tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca tcgatgcctg
     5161 cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta ttaaatacga
     5221 tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca tagacagcac
     5281 cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat tccatcattt
     5341 tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc tgtataggtg
     5401 atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac acctgaatgg
     5461 atcgaccagc ttggcgaaat aatggcttgt tcattgtcta caactaaatg ttttgtttca
     5521 tctggtttgc ccatcatgtg gaacacacgt gtatcttcat tggcataatc aaagtagaca
     5581 taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc gcttcctggt
     5641 tctaaaatcg tataacccat ttgtaattga cagctttcgc atacatttgg atggatgtat
     5701 tggtagattt tacgttcatt cagtgtcagg ccttcaccag tctccattgg ttttacttgg
     5761 tcgatactga tttttacatt tggatattta tgatgagctg gaacagaact aatatagaat
     5821 ttagctgggt tttcagcatc ttgagaagaa aatacaacat gtcgtgtttc ttttccgata
     5881 tagtatccgt cttgtttttt catttcttct ttttgcccat cgatctcgat gaatcctggt
     5941 ccaccgatat tgatcacacc tagttcacga cgctctaaga agtaatcaac acctagttct
     6001 ttgtctaatg tgatttctaa ttctttttca gtaggtgtca ctccaccaaa gatcattcga
     6061 tcgttgtgcg tataagtcaa actgattttc cctggttcaa ataccttttc tactaaaaat
     6121 tcttttctca attgttccgt tgaataatga cggatatctt ctggactgtg tgtataacgt
     6181 gtttccattt cttgcatttt cttctactcc ttctatcgaa tgatttttcc agagccgcat
     6241 gctaaaaatt gtgcgacttc ttcttgacta aattggttgc agtccccatg gatcgtatgc
     6301 ttcaaggcag aagctgccgt agcaaaatca atgatttctt gcggagacat attggataaa
     6361 atcccatgga gcacgccacc tgcaaaagca tctccgccac ctactcgatc cacgattggg
     6421 gtgattctat ggacttggga ttccacatat tcatggttca tccacaatgt ccccttcaat
     6481 tcattttcac ttgctgaatg aacggtacgc ttggtcgaat aaaacacctg aatattcggg
     6541 aacttttcct gcattttttg ataataataa attagaggtt gttcttctgc ttctggcgct
     6601 ttctcgatac ctaacaaata aagagcatcc atttttccag ctgagcagat atctactaaa
     6661 ggtaaaagct gatggattac ttcgctggct tcttgctgtg tccataattt tccacgatag
     6721 ttgatatcga agctgatcag gcagccagct ttttttgcag cttctatcac ctgtttcgtc
     6781 attttttgcc attttttaga caaagctggg gtgattcctg aaacatgaaa tatatctatt
     6841 ccttggaata actcctctag cgaccattcc aaatgatcca tttcagcaaa acttgatccg
     6901 gcacgatcat agatcactga ggcggcgcgt tcgccactac cagtttccat ataataagta
     6961 cctagtcttg atccaccaga taatacctgt ttcgtatcta ctccataaca ttgcaagtgc
     7021 tttttgactg cttttcccaa tgcgttatca ggtacttttg tagcaaaaat cgcttcatgt
     7081 ccgtaattag ctaaagaaat tgctacgttt gcttcccctc cgccataatg ggcagcgaat
     7141 cgttcacttg aatcgatacg ggttccctgc tctgtggaca atcgcaacat gatttctccg
     7201 agtgtcacta cttttcccat aagcagctac ctcttgattt ctttcatttt tgctgcatac
     7261 tgttgcgcga cttccgttac tttgtcaaaa tctcctgtag ctgcgggtgc taaaaggttc
     7321 ccacctactc cgactgttac gacaccagca tcgaaccact ctttcatatt gtccagactc
     7381 acaccgccag ttggcatgat attgacttga ggcatcggcg ccttgaaggc agaaatgata
     7441 cttggtccat aagcgcttcc aggaaatagt ttgacgatat ctaccccgct tttcagcgct
     7501 gttttgatct cggtaatagt catacatcct ggtaagtaag gaatttgata cagattgcac
     7561 atctctgccg tttcttgatc aaaggaaggg ctaacaatat actctgcgcc agctaagata
     7621 gccaagcgag ctgtgacagc gtctaatact gtgcctgcgc caatcactat ttctggttgg
     7681 tctttgtaga atgacaaaag ttctttgatt acttgatctg cttgagggac tgtaaaagtc
     7741 aactcaatac cagttagacc acctttgatg attgcgtggc aagcattcaa agcttcttct
     7801 ttgctttttc cgcgtaccac cgcaatcact ccggcgtttt tcaatcgaga taaaatatca
     7861 atttttttca tcttctccac ctatttccta tttagaaatt ttattttata acttggaaat
     7921 ctatacccat attatacggc agaaaagaaa aattgcaaac gcttttttca ttatttttga
     7981 attctttgct cgatttcgct ttttgtttgc ttgagggcac tgattatttg ttcttttgtc
     8041 tcatctgtta aacgatattt cggcatactt acactaaatg caccaatgat ttgttcgttt
     8101 ttcacgattg aggctcctat acaaaaaata tcacgttcga tttcttcatc gtcaaatgcg
     8161 atgtgggttt cttttaccgt tttgatttcc tttttcaacc gtaatgcatt ggtgatcgta
     8221 tattctgtgt aagggatcaa cggatgcttt tcaagataat cagtgtactg ctcttcactg
     8281 aattctgcta atacggcttt gcccatcgca gaattgtata atggtcgggt gattccgatt
     8341 tttgaagaca tgcggatcgt ttgatgtttt ggatctagtt tgttcacgta caagatctca
     8401 ttgttgtcca atacccctaa gtggatagtc tcatcgatct tttgctgcag ctgttctaaa
     8461 aacggtaaag tcaactctac caaatcgatt tgttcaatgt ttttattcgc gtagcgaata
     8521 agctttgcac ctaaacgata gtttttctct gaatttttat ttacgtagcc gatcatcaac
     8581 aaagtatcta gtattttcaa gactgtagaa gaggtcattc ccacaccctt tgcgatcgtc
     8641 tgcaaagaga catcttggtt ttccgctaaa tagtctagta ttttagaggc tttgatcaat
     8701 acagttccat aaggc
//
LOCUS       DAZKNM010000115         8710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000115 DAZKNM010000000
VERSION     DAZKNM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.116"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(308..2689)
                     /locus_tag="ACG8M9_002075"
     CDS             complement(308..2689)
                     /locus_tag="ACG8M9_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0378802.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2679..3326)
                     /gene="recU"
                     /locus_tag="ACG8M9_002076"
     CDS             complement(2679..3326)
                     /gene="recU"
                     /locus_tag="ACG8M9_002076"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0378803.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3390..3929
                     /locus_tag="ACG8M9_002077"
     CDS             3390..3929
                     /locus_tag="ACG8M9_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0378804.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4019..4438
                     /gene="gpsB"
                     /locus_tag="ACG8M9_002078"
     CDS             4019..4438
                     /gene="gpsB"
                     /locus_tag="ACG8M9_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0378805.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4460..4827
                     /gene="rnpB"
                     /locus_tag="ACG8M9_002079"
     ncRNA           4460..4827
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8M9_002079"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5036..6202
                     /locus_tag="ACG8M9_002080"
     CDS             5036..6202
                     /locus_tag="ACG8M9_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0378806.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6248..7744
                     /locus_tag="ACG8M9_002081"
     CDS             6248..7744
                     /locus_tag="ACG8M9_002081"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0378807.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7825..8508
                     /locus_tag="ACG8M9_002082"
     CDS             7825..8508
                     /locus_tag="ACG8M9_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0378808.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8517..>8710
                     /locus_tag="ACG8M9_002083"
     CDS             8517..>8710
                     /locus_tag="ACG8M9_002083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378809.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2742 a         1575 c         1845 g         2548 t
ORIGIN      
        1 ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt aagaagtggc
       61 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
      121 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      181 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      241 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      301 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      361 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      421 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      481 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      541 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      601 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      661 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      721 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      781 tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      841 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      901 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      961 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
     1021 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1081 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1141 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1201 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1261 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1321 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1381 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1441 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1501 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1561 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1621 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1681 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1741 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1801 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1861 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1921 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1981 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     2041 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2101 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2161 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2221 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2281 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2341 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2401 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2461 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2521 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2581 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2641 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2701 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2761 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2821 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2881 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2941 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     3001 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3061 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3121 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3181 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3241 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3301 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3361 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3421 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3481 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3541 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3601 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3661 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3721 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3781 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3841 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3901 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3961 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     4021 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4081 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     4141 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4201 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4261 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4321 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4381 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4441 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4501 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4561 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4621 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4681 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4741 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4801 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4861 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4921 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4981 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     5041 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5101 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5161 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5221 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5281 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5341 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5401 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5461 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5521 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5581 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5641 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5701 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5761 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5821 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5881 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5941 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     6001 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6061 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6121 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6181 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6241 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6301 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6361 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6421 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6481 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6541 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6601 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6661 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6721 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6781 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6841 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6901 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6961 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     7021 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7081 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7141 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7201 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     7261 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7321 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7381 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7441 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7501 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7561 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7621 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7681 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7741 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7801 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7861 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7921 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7981 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     8041 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     8101 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8161 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8221 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8281 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8341 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8401 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8461 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8521 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8581 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8641 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8701 accgtttatt
//
LOCUS       DAZKNM010000116         8486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000116 DAZKNM010000000
VERSION     DAZKNM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.117"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..555)
                     /locus_tag="ACG8M9_002084"
     CDS             complement(25..555)
                     /locus_tag="ACG8M9_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0378810.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(654..1004)
                     /locus_tag="ACG8M9_002085"
     CDS             complement(654..1004)
                     /locus_tag="ACG8M9_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0378811.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1161..2690)
                     /gene="ahpF"
                     /locus_tag="ACG8M9_002086"
     CDS             complement(1161..2690)
                     /gene="ahpF"
                     /locus_tag="ACG8M9_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0378812.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2700..3263)
                     /gene="ahpC"
                     /locus_tag="ACG8M9_002087"
     CDS             complement(2700..3263)
                     /gene="ahpC"
                     /locus_tag="ACG8M9_002087"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0378813.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3407..4069)
                     /locus_tag="ACG8M9_002088"
     CDS             complement(3407..4069)
                     /locus_tag="ACG8M9_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0378814.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4253..5899
                     /locus_tag="ACG8M9_002089"
     CDS             4253..5899
                     /locus_tag="ACG8M9_002089"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0378815.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5890..6972
                     /gene="uxuA"
                     /locus_tag="ACG8M9_002090"
     CDS             5890..6972
                     /gene="uxuA"
                     /locus_tag="ACG8M9_002090"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0378816.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7080..8486)
                     /locus_tag="ACG8M9_002091"
                     /pseudo
     CDS             complement(7080..8486)
                     /locus_tag="ACG8M9_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2712 a         1562 c         1540 g         2672 t
ORIGIN      
        1 gtctatttta ataactaaaa atgattaacg tatagtcatt tttcttattc tgatgaaatt
       61 gataatctcc tggatgccca tgaaaatcaa aataaagccg aatatttgga atagaagcat
      121 caaacttttg aatggattga acaataagac gaatccgcca atcaacaaca gaatactact
      181 ataaacactc caacttttag cttttgtatt catgctgaag aacaactgga acaagctgtt
      241 gatgataaca gctaatccaa gaagaatcgg caggatagaa acgattgcag gtgcaaaagc
      301 atagacgatc agagctaaga tcaggaaaaa aagaccactg aacagaccga tactccaaga
      361 acctgttttc cttttaaaac ggtaagcctc aaaaagattc aacaacccaa gcaaaacaag
      421 ataggcggtg atcagataac cgataaaatg aaaaacagca gaagggttta gcacaatcgc
      481 aatcccagaa ataatataaa cagcagctct taataaggcg tatttttgaa tattttgttt
      541 aatcgtctca aacatataag aaaacctcct ttgacttatt ttaacgtgaa ctagtcattt
      601 taccaaatta aatgttacaa aatgagcgaa ggagaagaat atctctggaa aaatcaactt
      661 ctgaggatct tttccatttt tttgcctttt gctagctcat cgatcatttt atctaaataa
      721 cgaatttctt tcatcaagtt atcctcgatg tcttctactc gtacaccaca aacaacacct
      781 gtaatcaagg aacgtgaagg attggggaca ggggcttccg caaaaaattg ttcgaaagtc
      841 acttgttttt gtagttgata ttctagttct gtttgggaat aacccgtcag ccaacaaatg
      901 atttcatcta cttcctcttt tatacgatct tttctctccg cttttgctac atacatagga
      961 taaacgcttg caaaacttgt cgtaaaaata cgattttgcg ccataatcag tcacctttct
     1021 ttttttcaaa ttatttacta cttatagtat ataagaaaaa gagaagaaaa agcgattatt
     1081 caaaaaaaca aaaaggattg tgacaaaatt cttgtcacca tccttttttc cgctctctga
     1141 ctttttacaa tcttcatagc tcaagtgcgc atcagataat cgaatgctcc taaagctgca
     1201 gtagctcctg aacccattga aacaatgatt tgtttgtatg gactatctgt acaatcacca
     1261 gctgcgaaaa cgccggggat attcgttgcg ccatttttct cggtcactat ttcatttctt
     1321 ggattcattt cgatcgttct atctagccaa tccgtattag ggagtaagcc gactagaatg
     1381 aatactcctg aaacagctag tgtatgcatc tccatcgtac ggcgatcgat ataggagaga
     1441 gattctacct gattcgtacc gctgatttct ttcgtcgctg catgtttgat aacagtgaca
     1501 ttcgcaagag agactagttt atcctgtaag atttgatctg cttttaattc tggtaagaat
     1561 tctagaacat atacatgttt tgctaaccca gccaaatcga ttgctgcttc aacaccagaa
     1621 ttaccaccac cgataacagc aatctctttt cctttaaata aagggccgtc acagtgtggg
     1681 caataagcaa ttcctttatt tttaaactct ttttcgcctg gtacattgat agaacgccag
     1741 tgcgcacctg tcgcgatgat ggcggttttt gctttaagtg atataccgtt agctaagcca
     1801 atattaataa aatgttgctc tttatttaag gaaacggcct gttgattcat cataatgtcg
     1861 atagggtaac tacgcacatg actttctact tgctgcatca attttggacc ttctgtgtaa
     1921 gaggtaccaa tcatattttc aatccctagt gtttcaacga cttgtccgcc gaaagaatca
     1981 gcgacaagtc ctgtacggat tcctttacgt gctgcataga tagctgcact actagctgct
     2041 ggtccaccgc caatcactaa tacatcataa ggttcttttt caaacaactc ttcttcaatc
     2101 aaagggccag tgattttttc aacaatttgt tcgatcgtca tgcgtccgct actaaattct
     2161 tctccattta aaaacacagt tggcactgcc atgatttttt tatcatcgat ttctttttgg
     2221 aacatcccac cttcaatcat cgtatgagta atatttggat ttaatacagc taatatattc
     2281 aatgcctgta caacgtcggg gcaattatga caagtcaagc taacataact gtcaaaatgc
     2341 aatgtttgtg ttattttttt gattttaaga ctcaaagatt cactgatttt cggtgcccgt
     2401 ccactaattt gtaacatagc cagtaaaaaa gaggaaaatt catgaccgag ggggatacca
     2461 gcaaaaacga ttccactttc tttgccaact ggattgatag aaaaactagg tgtgcgcatc
     2521 gaatctcttt tttcccaaga gattttagga gacatttctg ctatttcctt cgtaaattca
     2581 gctagttttt gactattttc actattatcc aaactaacag aaaaaacaat aggagattca
     2641 atcagtgcta aatattgttc gagattttgt ttagtttctt tgtctaacat atccaccacc
     2701 taaatctttc caacaagatc aaaacttggt ttcaatgttt cgccactttc tttccatttt
     2761 gccggacaaa cttcacctgg gtgggtacgt acatattgag cagcgcgaat cttatcaatc
     2821 agtgcactag cgtcacgacc gattccatct gcattgattt ccatagcttg aacgattccg
     2881 tctggatcaa taataaatgt tcctcgctga gctaatcctt gctcttcatc caaaacatca
     2941 aaaatacgag aaatctgatg agacggatca ccgatcatca cgtattcgat tttaccgata
     3001 gcatctgatg tatcatgcca tgctttgtga gtaaagtggg tatcggtaga gcaagaaaag
     3061 acttcaacac ctaaagattt caatgtactg tattgttctt gtaagtcttc taattcagta
     3121 ggacaaacaa aagtaaaatc agctggatag aagcaaagaa tactccagtt ccccataaga
     3181 tctttttcac tgacttctaa aaattctcct tgatgataag catgtgcatt aaattcttcg
     3241 atttttttcc caatcaatga catagtaaaa cctccaataa taattattta tttataatta
     3301 ttgtaattta gttggagttg caaagtcaat ggatattttg ttatttaata aagattactc
     3361 aaaaacgaag gttaattata aaaatagaag aggtcgcaag aaaatgctac tgctggaaat
     3421 actcgggata acgttgagta acagtttcct gttctttcag catcatgtgt aagtgcaatc
     3481 ccgtttgaaa tcgagcttct tctggttttt tatcaactaa agccttgagt aaatcataat
     3541 gttgttccat gatcgtttcc catggaagcc cctgaatggt cactcgtaac cagcggaaac
     3601 gttctaaatg ggtattatgg tcatctaacc agttccaaac ttcttttctt cctgcaattt
     3661 cgaaacaagc ttcatgaaat aaattatctg cacggaaaaa agcgcgttcg tcatttattt
     3721 tgactgcttt ttcttgttct tctaaaatcg tttctagcac tattcgtgtt tgtgtgtcca
     3781 atttggaaca acattcaatc atgatttgct cttccaactg ttctcggaca aagcgagcat
     3841 tttctgcaga aacaagatta attttggaaa catacgtacc actttgaggt atcgtataca
     3901 cgagttcttg ctgacgtagt tgaatcaatg cttcgcgaat gggagtccgg ccgatagcaa
     3961 gatgttcctc cagtgccttt tcagatattt tccttccagg ttctaagtca gcatagataa
     4021 tttgtttacg aatcgcttga taagcttgtg attgcaagct gcttttcatc tttattcctc
     4081 caaaatcatt tctatgaacg tagtataaca gaagaggtct gaaaaacaat ctggtatgtc
     4141 acattaacta gtaaatttgt ataaatagta aaattttggt tgacatagcg atttttattg
     4201 cataaaattc acttgaatac aaaaactaat atgttagttt aggaggggaa aaatgattga
     4261 tttaaacaac tggaggaact ttatccctga attatctcaa aaagaagtag aaatacctca
     4321 ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat cctgtctgga tccatttcgg
     4381 tggcggaaat ctttatcgag gatttcatgc agaaatcgct caacgtttag cggatctagg
     4441 cgaattgtcc actggtgtca ttgtttgtga aacattcgat gaagaaataa ttgaaagggc
     4501 ttaccaagat tttgatgatt tgattcttga agtggtcatg ccaagcagtg gagagttcgt
     4561 caaacgtata ttagctgcga cagctgacag tctgtattgt catccagatt gttctgaaca
     4621 ctttgataag atgattcgac actttgaaaa tcctagttta caattagtta cttttgctat
     4681 tactgaaaaa ggttatcgat tgaaagatac aaataataaa tatataggac cgattgcagt
     4741 cgatataaaa aatggtccaa agaagccatc acatacaatg agtattcttg ctgtgctact
     4801 ttataaacgt tttttatctg gagcttggcc gattgcactt gtctctacag ataatttttc
     4861 acaaaacggt agtcaattac gtacttcttt gctaacgatc gcaaaagaat ggcaaaagga
     4921 aggttatgta caaaatacat ttatccaata cttatcgaat gaagaaattg ttgcgtttcc
     4981 atggacgatg attgatcgta tcaccccgaa tccttccttg tcagttagca aaatgttggc
     5041 aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca aaaggaaata atattgctcc
     5101 atttgtcaat acagaggaca tccattattt aatcatggaa gatcattttc ccaatggacg
     5161 tccgaaattc gaaaaagctg gggtattctt gactgatcgt gcaacggtag ataaagcaga
     5221 tacgatgaag gtgacgactt gccttaatcc tttgcatacg tctttagcta ttatgggttg
     5281 tttactgggg tataggagca tagctgagga gatgaaagat ccagatttat cgctgctagt
     5341 ttctgaaatc gggtacaatg aaggcctccc agttgtggag tctccaggta tcattgatcc
     5401 aaagatcttt ctggaagaag taattactgt gcgcttttct aatccaaata ttcctaatat
     5461 gcctcagcga atcgccacgg atacctctca aaaggtggcg attcgttatg gtgagacgat
     5521 taaaaaatat atggaaatag aagttaaaga cccgcagaca cttgtattca taccattgac
     5581 tctcgctgct tggcttcgat accttattgg cattgatgat caaggaaaac cattcatccc
     5641 gagtccagac ccactattga cagaccttca gcataagctt tccgatattc aactgggtac
     5701 gcaagataca tcacatattc atgagaaaat ggctcctatt ttgtcaaata aagaaatttt
     5761 tggtgtagat ctttaccaag cagagcttgg cagaaaaata gaagaaatgt tcatcaagat
     5821 ggtagcagga ccaaatgccg tacgaaaaac attacaggat tacttacaag aacatggagg
     5881 aaatttatca tggaaatgac ttttagatgg tacggatcac aggaagaaaa gattacctta
     5941 gatgaaatca gacagattcc tgcggtcaaa gggattgtag gcacattgat ggatatacca
     6001 gtaggagagg tatggcctag ggagaggatc aaagaactaa aagaagaaat tgagtctcgt
     6061 gggttaacac taaaagtaat tgaaagcgtc aatgtgcatg atgccattaa aattggatta
     6121 ccttcccgtg agcagtatat cgaaaactat aagcagacaa tccgaaactt ggcagaatat
     6181 ggaatagaag tcatctgcta taattttatg cctgtatttg actgggtgaa atctgacctt
     6241 gactaccgct tagaagacgg ctcatccacg ttagctttta tatctgatga tattccagat
     6301 gatccaagag aaatcgtcgc gcgcatcgaa cattccagca atgaatttgg attgccggga
     6361 tgggaacctg agcgcttagc tcctatcaaa tccttgcttg aagcctatgc atcagtagac
     6421 gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat caatcatccc aacatgtgaa
     6481 gaggtagggg tcaaaatggc tgtacatcca gatgatccgc catactcgat ttttggatta
     6541 gctcgaatcg taaagaatcg agaagattta gattggttgt gtcgcatagt agacagccct
     6601 tataacggaa tcactctttg tacagggagt attgcagaag atccagaaaa tgatgtatat
     6661 gggattttag cagaattttg ccaaagagat cgaatcccat ttgcacatgt tcgaaatatt
     6721 aaatttttag ctaatcgaga tttctatgag gcaccacata aatccgaata cggttcatta
     6781 gacatgtacc gcatactaaa aacaatgtat gacaacggat ttaacggata catccgtcct
     6841 gatcatggaa gaatgatctg gaatgagaca ggaagaccag gatacggctt atatgatcga
     6901 gcattaggtg ctgtttatct aaatggatta tgggaagcaa tcgaaaaagg aaacaaaagt
     6961 aaagataatt agtgaaaaaa actgtaattt tattatgtaa ataagaagga tgttcaaatt
     7021 cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc ttatttggtc
     7081 taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc ttttaattca
     7141 aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt gatgattcct
     7201 tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca gtcgctgata
     7261 acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt tgaaaaaatt
     7321 tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata gttcacacca
     7381 atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc ttcaaccatc
     7441 cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg ttctctgaaa
     7501 tccatataaa attgttcaat ggcggtttgg acttctttca atacgctatg ttcttgataa
     7561 agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc attataaagt
     7621 ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat aaacaaatca
     7681 aatacagtac aaagggactc caccatttct cttgaataaa gaacatgagg aacgtttgct
     7741 ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt cgtgatagaa
     7801 agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag attaggtcgc
     7861 aaaatatcta cagagagatc aataaaaaag tcaatatatt catactctgt gaattctttt
     7921 tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat
     7981 aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc
     8041 atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag
     8101 atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac
     8161 tttctgccat tattagaaaa ggagatagac aaaccgtact atgctaaaaa tgcattaatc
     8221 tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact gaaactgata
     8281 atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg
     8341 cagtatcttc caaatagacg ataatagcag taagccggat caattccttc aaaattcaac
     8401 tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct
     8461 tgttctaatt ctttgatgta tagtaa
//
LOCUS       DAZKNM010000117         8402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000117 DAZKNM010000000
VERSION     DAZKNM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.118"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..541)
                     /locus_tag="ACG8M9_002092"
     CDS             complement(44..541)
                     /locus_tag="ACG8M9_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0378817.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(671..1381)
                     /gene="budA"
                     /locus_tag="ACG8M9_002093"
     CDS             complement(671..1381)
                     /gene="budA"
                     /locus_tag="ACG8M9_002093"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0378818.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1394..3058)
                     /gene="alsS"
                     /locus_tag="ACG8M9_002094"
     CDS             complement(1394..3058)
                     /gene="alsS"
                     /locus_tag="ACG8M9_002094"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0378819.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLGIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3263..3925)
                     /locus_tag="ACG8M9_002095"
     CDS             complement(3263..3925)
                     /locus_tag="ACG8M9_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0378820.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3935..4750)
                     /locus_tag="ACG8M9_002096"
     CDS             complement(3935..4750)
                     /locus_tag="ACG8M9_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0378821.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5012..5455)
                     /locus_tag="ACG8M9_002097"
     CDS             complement(5012..5455)
                     /locus_tag="ACG8M9_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0378822.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5589..5927)
                     /locus_tag="ACG8M9_002098"
     CDS             complement(5589..5927)
                     /locus_tag="ACG8M9_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0378823.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5915..6292)
                     /locus_tag="ACG8M9_002099"
     CDS             complement(5915..6292)
                     /locus_tag="ACG8M9_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0378824.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6516..7709
                     /locus_tag="ACG8M9_002100"
     CDS             6516..7709
                     /locus_tag="ACG8M9_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0378825.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7875..8297)
                     /locus_tag="ACG8M9_002101"
     CDS             complement(7875..8297)
                     /locus_tag="ACG8M9_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378826.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2465 a         1698 c         1414 g         2825 t
ORIGIN      
        1 ttttattctt gatttctctt cttttttgtt tcaatagacg attttagtta ttaataaatg
       61 aaaaagcatt tatattaaaa tttgctaata aaagatctac atatttttct agaaatgctt
      121 gatcgatttc atcataccct ttcgttactc cgcaatcgat atctaaaaca ccgattaaat
      181 taccttcttt taccattggc actacgattt ctgattgtgc ggctgaatcg caggaaatgt
      241 aatttgcatg ggttttgaca tcatcaacga tcaatgtttc ttgtttttca gctgattctc
      301 cacaaactcc ttttcccatc ttgatacgtg tgcaagaaac tcgcccttgg aacggtccta
      361 aaatcaattc attaccatcg aataaataat atcctgcaaa gacagtctca ggcaaagtct
      421 cattcaacaa tgcagatgaa tttgctaaat ttgctaacca gttatcttct gcttctaata
      481 atccttgttg ctgtaataat aaaatttcgt atgcatcttt tttctgtttt tctgtccaca
      541 tgataaaaac cgctcctttt tgatctatgg agaagattat agctcagttt ttttgtaaac
      601 gctaggtttc ctcctcaaaa aaacgagata cgtcaggtta atcaccttga acgcatctcg
      661 ctgtcttttt ctattctgct tgttggattt ctgctgctaa atcagcataa tcaatcgttt
      721 cgtgaacaaa agtcggatca tcaactggaa aatgctgtac aagggacgag atgcggctaa
      781 tctcaacttt tccttcagcc acttcaaaat ccattacatg tccgccaaat gtatgtgtat
      841 catcgataaa atgcaagtga aatcccgctg cagcaactcc ttggaataat tcaggcgtaa
      901 agaacccgac taatgttcct tggacattgc tttgttggaa ttctggttga gactctgaaa
      961 tttttgctag tcttctgtaa ggtttttctt gcttcggcat gatacggatg tgcatatttt
     1021 tgaataatcc agttatcttc actgccaaaa ataggttttc cccggcttct cttactaata
     1081 tgtctttttt caattgttct ccatctgcag tttttgagac agaaaaacta gtatctggcg
     1141 taaaatctgt aattgctgca tagggggtaa gttcacttcc atctaattgg gcaactgagc
     1201 catctgatct tccttgataa gctacgccat ctaaaaagat gacttcacca tcaagcccat
     1261 gcaatgttcc caatcccaga gttccttttt ctaatatatg agcaatcgtt tctgttccat
     1321 ccatcaagcc agccatcaaa gctcctaatg tcccgtgctg gtataagatt ttttcagtca
     1381 tgtcgacact tccttaatag aattgatctg gtaagattgt ttctcccaat ttctcattat
     1441 cacggtaatc aatcggaata tcaataatta cgggtccttc tgtcgcaaat ccttcttcta
     1501 aagctttttc taattcctct acacttgtag ctcggattcc tttagcgcca aaagcttctg
     1561 catatttcac aaaatcaact ggaccaaagt ctacacctga cgcccgctga tatttcattt
     1621 tttcttggaa ctctaccata ttgtagtgtc catcattcca aatcaaatga acgatattca
     1681 attttttacg taccgccgtt tctaaatcct gggcagagaa taaaaatccc ccatcgccgg
     1741 aaactgatac gatttgtgta tttggacgca ccaatgcagc agaaatcgcc catggcaatg
     1801 caacaccaag cgtttgcata ccattactga ataacaaatg acgaggttca taacttctaa
     1861 agtggcgtgc catccaaata taatgactac ctacatctac ggtaacagtc atatcatctg
     1921 ttacttttga ctgaagcgtg ttaataactt ctaaaggatg caagatacct gcttctcctt
     1981 ttgattgaac aatgtcccgc tccgttaatt ctgcttgaag agatgctaaa tattcttttg
     2041 catcttctga cacttgttga ggctctaatg aacctgtcaa taaatcaaga gtggctgaga
     2101 tatcaccgat caattctctt tcaggctgca taaaaggatc gatttcagct ggcgcttcat
     2161 cgatcacaat gatccgcgca tctttttccg cattccagtt tcttgcttca tattcgattg
     2221 gatcatagcc aatagcaata actagatcac ttcgtttcaa aagcatatct cctggttggt
     2281 ttctaaataa acccacacga ccaaagaaat gcgcttctaa ttcacgtgag atcactccag
     2341 ctgcttggaa tgtctctaca acaggcaatg ctgtctttcc aactaattgc ctgatgccta
     2401 acgtttcttt ttcacttgat cctcgcatac ctactaatag taccggtagt tttgcttctc
     2461 taatgcgttc agctaggtaa cggatatcat ctgcagaagc agaacctaat ttaggatcgc
     2521 tcaatggctt gatgacattc ccttggacag gtgcatccac tacatcttgc gggatactga
     2581 taaagctcgc gccttttttt gaactcttag ccatacgata agcattggca atcacttctg
     2641 aaattgtttc tggatcttgg atttctgcac tgtattttgt gattggctgg aacagtgctg
     2701 cattatccat gctttgatgg gtcaacttca atagatcgct tcgttttact tgtccggcaa
     2761 ttgctaaaac tgggtcacct tctgctgtcg ctgttaccaa acctgtagct aagttactag
     2821 caccaggacc acttgttgcg ataactactc ctggctctcc tgtaatacgt ccgattgctt
     2881 gtgccataaa tgcagcattt tgttcgtgac gagtcacgat taattcagga ccttgatcct
     2941 ctaattcatt aaatacacca tcgatttttg cacctggtat accgaataca taatcaacat
     3001 gatgattaat caaactttct actacagatt tcgaaccttg tttcattttt tcgtccattg
     3061 aacatcgtcc tttttatgct ttatttttaa ttattagtta gaaataatca caaataattg
     3121 atacagtttc taaacctgtt tcattcaaaa gtatagcacg ttttatttca tttgtttagc
     3181 catatgtttc aaaaaatgaa aaagaatgac agtttttaca taaaaactgt cattcttaat
     3241 tgaaagtttc aatttataaa gtctacagat ccatagttct catcaattta cgattgttga
     3301 agtaaatcaa accaaatacg atagaaggac ctagtagaat acaccattgg ggctttacga
     3361 aaaagaagag aaacaaactt gtcaatagta aaaaatctag taaactcaca taccattttt
     3421 tcaacgtata gtagaacgct atccgtagaa cttgatttgg tttcgacgct ggatttctca
     3481 cctgaaaata ccagtagttg atgctaaagg aaactgataa acaagcaagt aaaacaaaca
     3541 acggaatcaa ccattttcca taagaaaaac gtataaaaaa gaaacaatca aaaatcatta
     3601 tcacactaac tatccaagca aatagccaat aaaaaaatcc ctttagccaa aattggcgaa
     3661 acgtctttat gaacaaacgt actggtgaaa aacttttttc ttttgctaat tcttctgtca
     3721 tacctatcat cacgatgcaa cctggaccaa taaaaagtaa agaaaaaata aaggaaaaga
     3781 cattccttcc atctatcgac aaaaggaacg cagctgctat atacaagata ttgacacacc
     3841 aaaaacaaag gctacttgcc aatgattgat acaaccaata aaagaattcc atataaacat
     3901 ttttgtcaaa acgctgctct ttcataatca cctcctattc cttcattcct gatgaagcga
     3961 tcccttctac aaaatatttt tgcatcgcta aaaatataaa agcaacagga agaacagaaa
     4021 caactgaggc agccatgatt aatgcgtact ctgcatcaaa tagcccaata aacattttca
     4081 accctaactg gattgtttta ttttccgtag aagacaaata aatgaatggc cccatataat
     4141 cgttccatgt attaacgaat gtgatgatcg tcaaactggc aatcgccggt tttgtaagag
     4201 gtaaaatgat cttgcgatag attccccact ctgataagcc atcgatccgc gcactctcac
     4261 ataaagaatc tgggatacta ctgtagtatt gcttcaacaa gaacacacca aaagcattga
     4321 acgcttgtaa tagcaccaac gaccacaacg tatatgttaa acccactttt ctcatcataa
     4381 tgaactgcgg gatcatataa gactgccaag ggacagcaat cgtgccgata tatgctaaaa
     4441 ataaaatatc gcgcccttta aaatttagtt tagaaaaccc gtaagcagca aaactagaag
     4501 taaacaactg aatcaacgtg atcacaacgc ttaaaaaagc tgtattctta aaaaatgtca
     4561 ctaaaggaat acgagaccaa ataaccagat aattctctaa atgccaagtt ttagggatcc
     4621 attgaatcgg aatcgtgaac acttcattat ttgctttgaa tgaagcagaa accatccata
     4681 aaaaaggaat gatcgtcaat aaagctaata caatcagcag aatcataccc aaccattgaa
     4741 taaagggcat gttttttgaa cgaatttttg ctttttttct ttcatttcga acacctccaa
     4801 agttataatc ttcatttctt tgtaatgatt acaatccaga acaacggtta ttatagctga
     4861 ttcatttccg ttcttcttat gtttgtaagc gcttatttaa caagttatgt aaatctcttt
     4921 attttttttg atttaaaaat gaaaaaatac aaaagcaaga ctgtcatacg tctcgctttt
     4981 gtattgaaat ttcttcgatt agagatactt attactgttc aaaagcttct ttgaaacggt
     5041 gaatcaattc attgactaca ggtgataaaa tccgattctt tttccagact agcatgcagt
     5101 tcgtttcaat cattgggtca agcggaacga atgtcactcg ctttgaaaga tgacttacag
     5161 caccttctat gactaaagca gcacctacct gattctcaat tagcgcaaat acattgaaaa
     5221 tcaaattgta cgttcctgtg atcagcaact gctccttttt tatgcctaac cagtcagcaa
     5281 acattcgctg tatttctgga cgctttgaga tcaaaagagg tacattcgca aagtcttctt
     5341 ttgttatcac gtcttttttt gctaaaacag aattttctaa aatgagcaat ccccattttt
     5401 cctttcttgg taacaaaatc gtttcgtatt tctctgtatc tactggatct agcaaaatcg
     5461 ctaaatctaa caggcttttt tctagtcact ccgtgatatc atcacttgtt ccgctgtgga
     5521 tatgaaattg aacgctggga taatcacgga tcaacttttc tagtaatagt gccaatgtat
     5581 tcgaattatc agcttccaca cagccgatcg taaatcggcc gctgaataat tgtttttttc
     5641 tgttctcgaa ttcttgagtg gtttgattcg ttaaatctaa aatttcttct gctcggcttt
     5701 tttagaaaat aacctgcttc tgtcaagatc attttgcggt tttcgcgtac aaacagttct
     5761 gttcctagtt cctcttctaa ctccttcagt tgtcggctta aggttggttg tgtaagatgt
     5821 agaacttctg cggcttgaga gattgttcct tcttctgcaa ttgtccaaaa atattttagt
     5881 aagcgtaact ccatagcgat gctccttgat ttttttactt gatacattcc attttatttc
     5941 tagataacac acgatcatat ttcttgattt ttccttgtaa acgttccaaa cactcttgca
     6001 tttcagcaat ttctttcgtc aaaagttcac tttcttcaat caaaagctta cgccgctcaa
     6061 atacagttgc ttctccttcg ctagataggc gaatatattc aatcaaagac tcgatcgata
     6121 atccggcatt tcgcatacag atcgtaaagt cgacccattt taggtcttct tctgtataat
     6181 cacggattcc gccttttgtc cgattaactg gtggaatcaa cccgattcgt tcataatagc
     6241 gcaatgtatc ggccgaaatg cccttttctt cactgacttg tttgatattc atctgatcac
     6301 tctttctctt aatttcttgc cactttactt atgtatttta tttattctac aactttaagt
     6361 gtactccaag tcaatactca tttctattaa aactaagctg gattatctat atcggttgtt
     6421 tgtgtattat ttcaaataaa aaagtcttgt ttttttattc ttttttggat atacttttaa
     6481 caatttaggg aaattttagg aaaaggggta taaaaatgac aacgtatcag cgaaaggtat
     6541 tattatcgac atcggcggga attgctttag agaatatgga tattatgttt ttagcttttt
     6601 cattatcttc aatgattact tcttttcata ttagtggtac gcaagcgggt ttcatttcaa
     6661 cgatcactaa tttggggatg ctcattggag gtattttttt tggcatcata gctgataaat
     6721 atggacgtgt gaaagtattt tctcaaacgg tccttctgtt ttcgattgct tctttattga
     6781 tgtattttgc ttcaaacatc tacttggttt atctctttcg ctttatcgca ggtgtaggag
     6841 cgggaggaga atacggggcg tgtatgtcct tgatttccga aagtttttca aaaaaacaaa
     6901 ttggacgagc ttcttcggtt gctggtattg gtgcgcaggt cggtgcggct ttggctgcga
     6961 ttttagctgc tgtgatcatt ccaacgtttg gttggaagat gttgtatgtc gtaggtgtac
     7021 tgccggtgtt gatggtcttg ttcattcgtc gcggcttgac tgaaccaaag gaattcaaat
     7081 ctgtacaatc tgccgaaaag aaaaccaaac ttagcagact gtttgaatca aaacatcttg
     7141 cttggcaaac atgtggcctt agtatcatgg taactgtcca aattgccggc tattttgggt
     7201 taatgaattg gctgccttca attatgcaaa atcgattagg gctttcggtt tctgggtctt
     7261 cattgtggat ggtcagtact attttaggca tgtctttggg gatgatgact tttggcataa
     7321 tcatggataa attggggcct aaactagcct tttctttgtt tttaatttgt tcatccttat
     7381 ctgtctatct acttgtgtta gcccatagcc agtggagttt ggtagcagcc gctgtgatag
     7441 tcggctactt tatcaatggc atgtatggtg gatatggcgc agtgattagt agtttgtatc
     7501 caacagaaat ccgcgcgacg gcgaataatt tcattatgaa ccttggacga gctgttggtg
     7561 gtttttcttc tatcgtcatc ggtttcttga tggatcatta tagtttaact gccgtaatct
     7621 tgtttttgag cggtatttac ctagtcagtc tgctggtctt gacaactctt actggtgtta
     7681 cagcattaag ggaaagcttg cagcattgat agttgagtct gtaaatgaca agaaagaggt
     7741 gtggcaaaag cttgcatctg cggtaataag accagccaaa tcaaaaacat gagacactgt
     7801 ttccgtttgg ctggtcgtat tacttgatgc agggcagctt tttcaactgc taatttcctt
     7861 ctatctggcg gcgactattc tttgacgtcc gtcatttcaa agtactttac taataactta
     7921 gcataacgaa aaactgtgga tacactcgta tattgagcgt ctgcataatc ttccaaattt
     7981 ttctcaggat gttgatacat gaattcaatc aaatgataat tatatgattt tctgtagaaa
     8041 tgaagataaa taatgtagat tgggaagttc tcctgctttt ccatagaaaa gcgccgatta
     8101 ttctcattct tcaaaatgac cttatcagga tatttttgga acaattcttc taaatctgtt
     8161 agataattca agatagactt acttgatacc ttcaattcgt tttgtagttc ttctattgaa
     8221 caccaacgtt ctgtttcaag cagtaaatga atcattttaa ttttcttttg agaaactttt
     8281 gttaaaaaag tctgcatatt ttctcctcct gtatctactt tactatacta catacaagaa
     8341 aaaaaacgga tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat
     8401 tt
//
LOCUS       DAZKNM010000118         8249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000118 DAZKNM010000000
VERSION     DAZKNM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.119"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..2682
                     /gene="clpB"
                     /locus_tag="ACG8M9_002102"
     CDS             73..2682
                     /gene="clpB"
                     /locus_tag="ACG8M9_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0378827.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2833..3519)
                     /locus_tag="ACG8M9_002103"
     CDS             complement(2833..3519)
                     /locus_tag="ACG8M9_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378828.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3497..3991)
                     /locus_tag="ACG8M9_002104"
     CDS             complement(3497..3991)
                     /locus_tag="ACG8M9_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0378829.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4011..5231)
                     /locus_tag="ACG8M9_002105"
     CDS             complement(4011..5231)
                     /locus_tag="ACG8M9_002105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378830.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5378..7213
                     /gene="lepA"
                     /locus_tag="ACG8M9_002106"
     CDS             5378..7213
                     /gene="lepA"
                     /locus_tag="ACG8M9_002106"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0378831.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7409..>8249
                     /locus_tag="ACG8M9_002107"
     CDS             7409..>8249
                     /locus_tag="ACG8M9_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378832.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIP"
BASE COUNT         2794 a         1490 c         1676 g         2289 t
ORIGIN      
        1 aagcgatata atagtttttg taaagatcaa agaaataagt gatcttacct ttaattagga
       61 gggtatacct gtatgaatat tgagaaaatg actacgacgc ttcaagaagc gattgctgaa
      121 gctcaacaga ttgcggtcac tcgaaaacat caagatatcg acattgccca tgtatggaaa
      181 atctttttac aatctaatca ttttggtcga aatttctata cggatgccgg attggatgtc
      241 gaatcttttg aacatgaaat tgatcgtcta ctggatgagt atccagttgt ttctggtgga
      301 aacgtccaat atggacaaaa cctcagccag aatttctttc gattattgaa tgaagcagac
      361 caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg aagtcgttat tttagcgttg
      421 atgaaattaa aaaattatcc gttaactgtt tatttgaata aaaacggatt atctgaaaaa
      481 gaattgcgta aaaacatcga agagatgaga ggaggagaaa gagtgacctc acaaaaccaa
      541 gaagaacaat acaaagcatt agaaaaatac ggcgttgatt tagtacagca agtcaaaagc
      601 ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc gtgatgtcat ccgtattctt
      661 tcacgaaaaa caaaaaataa tcctgttctg ataggagaac ccggtgtagg gaaaacggca
      721 atcgttgaag ggcttgctca acgtatcgta cgcaaagatg tgcctgaaaa tttaaaagat
      781 aaaacgattt tttcattaga tatgggtgca ttgatcgctg gagcaaaatt ccgtggtgaa
      841 tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa aaagtgatgg acgtatcctc
      901 ttgttcatcg atgaaatcca caatatcgta ggggcaggaa aaacagaagg aagtatggat
      961 gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat tacatttgat cggtgccaca
     1021 actttagatg agtaccgtca atatatggaa aaagacaaag cgttggaacg tcgctttcaa
     1081 aaggtattag tcaaagaacc aacagtggaa gatacgatca gtattctgcg tggattaaaa
     1141 gaacgttttg agatccacca tggtgttaac atccatgaca acgcattagt agcagcagct
     1201 actttatctg accgttatat tactgatcgg ttcttaccgg acaaagcaat cgatttgatc
     1261 gatgaagcca gtgcaacgat tcgggtagaa atgaactcta tgccaacgga attagatcaa
     1321 gttactagac gtttgatgca gttggaaatc gaagaagctg cactgaaaaa agaatcagat
     1381 gatgcaagta aaaaacgctt gaaaaatttg caagaagaat tagctgaact tcgtgaagaa
     1441 gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag aagtcaattc tgtttcagca
     1501 aaacgtgctg aaatcgataa agcaaaacac gaattagaag atgcagaaaa taactatgat
     1561 ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc aattagaaaa agaattgaag
     1621 gaattagaag ctaaagcaaa agacagcgag attaagatgg ttcaagagtc cgttaccgaa
     1681 aatgagattg ctcaagtcgt tggcagatta acgggtatcc ccgtaacgaa acttgtagaa
     1741 ggtgaacgag aaaaactaat taaattgaat gaaacacttc ataaacgagt gattggtcaa
     1801 gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt cacgggctgg attgcaagac
     1861 ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa ctggtgtcgg aaaaaccgaa
     1921 ttagcgaaag cgttagctga gaacttgttt gactcagaag atcatatggt acggatcgat
     1981 atgagtgaat atatggaaaa acatgctgtg tctcgtctag tcggagctcc tccaggctat
     2041 gtaggttacg aagaaggtgg tcaattaacg gaagctgtca gacgaaatcc ttatacaatc
     2101 gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct tcaatatctt gttacaagtc
     2161 ctagatgacg gacgcttgac tgattcaaaa gggcgagtag tagatttcaa aaatacggtt
     2221 ctgatcatga ccagcaatat cggttcacag ttactgctgg aaggcgtgac tgctgatgga
     2281 acgattccag aagcagtggc tgaacaagtc aacacgctat tacgtggaaa cttcaaacca
     2341 gaattcttga accgaatcga cgacacgatt ttatttacac cattgagcct agacaacgtg
     2401 aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac gtctggaaca tcaagaaatc
     2461 ctgctgacaa tcagtgatga agccaaaacg tggattgctg aaaatgctta tgaaccagca
     2521 tatggtgcaa gaccattgaa acgatttatc actaaggaag tagaaacacc actagctaaa
     2581 gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa cgattacttt acttgatgga
     2641 caacttcatt tcaaaacaga agagctagaa gaaatcgtct aatcttccat tgagactata
     2701 aaagaaaaaa ggggtctgac acaactagtg tcagacctcc ttttttcaaa taaacagtgt
     2761 tccagaagta gtttcttcga aaaataagcc gaaatttctt gaagctgccc gcttctgtca
     2821 caacctcttt tattaggtat ttttcacttt ttctaattta taactttcaa agtaacgctc
     2881 atctttttta ctttgagacc atgcaaacaa tcccattaag gcagcaccta tcgtatttgt
     2941 cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg acaaacagtg tgccatcaga
     3001 agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg cataagaatt cccagaactc
     3061 ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat aagaggaaca accaaggact
     3121 ggtttttgat atctcagcat ctttcatcaa gtaaccaatc aatccatagc caagagctgt
     3181 caggaccatt ggactcaccg catgtaagat tttgtcccaa aaagagatga tactgatcaa
     3241 atgcatccct gtgcctaaaa agacagagat gaacaagaaa aaccagtaaa acaaaacgat
     3301 tgctgctggt attttaagtt tgaaaacttt caaaatgact tgtggcatga aaatcaatgc
     3361 aatccctaat aaagattcca cgataaatgt gattccttga gaagtcgaat atttttgtgt
     3421 gataaattca taaaaattat agcagacaac gaatacacca aaagcaagta aagctatttg
     3481 cacctttttg atttctttaa tcactgaaag tccctccatc taaaaatctt tttacttctg
     3541 aaagcaactc aatttctgaa gaaaccactt ttccgttcaa tttgatcaaa cctattgtat
     3601 ataagttcag gtagtgaaat tgattttcag ctgtttcttg tagtgcagcg atttttttcg
     3661 ggtgggttcc gccttgctgt cgagtatcag tatataagcc gatcactggg atgcctttcg
     3721 cataagccac accgatttcc gatgctaccc cggcatcgat cgtcagccca tctagtaaag
     3781 caatcattaa atcactagcc aacacttttt ctgtgtcagc aagagcaatc attcgactgt
     3841 ctgcataagc agttttgtca ttgattgctt cattttcttg aggaagatag atcgaaagct
     3901 cagaagagag ctctctgatt tctttaacta actgttggtt atataacaaa tcacttttag
     3961 caaacattgg tgcggcaaaa taaattttca tctaaacatc atccttttta tcaaaaataa
     4021 tcgctaaagt aagcttccat gtttggaatg atacgctcta gtgacttcag tgtttcttca
     4081 tctgtttcta accgttcgat tcttgccaaa taagaagcac ggcgataatt catcatcaag
     4141 caagtcaatg tttggatatc cagtcgaacg gattttccta taggttcatt taagacacgg
     4201 acttgtccct gttcatccca tgttaaagcg aagacaccat tattccaagg agcgacagga
     4261 tcttcaatga tgaaatgaaa agcatcagct gttccaacaa acggaaaccg ttgaaggaat
     4321 tcttttacat caacgatccg agccataaaa tatggttcga tctgctcttt gatctgacta
     4381 tcttcaatca aaaatgctaa aggctcattt ttataaatat ctccatgtac ccagtagatc
     4441 atggaaaaat gtgcggaaat aaagttccat aagccatttc gagcttcttg atcaagatag
     4501 aacatttctt ttacgtgaaa tatttcttca gctacccagt agaacagaac acctcttggt
     4561 tcatggttgc tatcgtagta gacagcggct gtgcgctcat cttcattttc gaaacgccaa
     4621 tactcttccc aatgaaaatc actgcggaat aaagcaccat ggttttgttg ggcaaatttg
     4681 tcataaacgg aaaatacgtc tggatgatca actggtaatc gctcaaccat accacttact
     4741 gggatttgtt taggtagctg tgtatctctg attttaaagg aaagtttatc agacatgatt
     4801 tcccatcctt tacgccgata gtaggggata ctgtaaggat acaaataaga gatccattgc
     4861 ttattcttgc gcatattatc caatgcaacg ataattagat cttgcatcaa tccatggtta
     4921 gcatattctg gataagttcc aactccagtc acaccaccca ttttataacg tgcaccgtga
     4981 atattgactt cacaaggata gatagcgatt tgagagatca gtttattttc gtggaaccat
     5041 ccgaacactt tcgatacttc caaaatcggt tttttggaac ggataaaagc tcgtttattc
     5101 tcaaacccgc tttcctcgat gtctgcttca gtcacttgga acacatagga aagcaattca
     5161 ttgaattgat cgatatgttc ttgagtgacc ggttttaatt caagattttc tctaaaatct
     5221 ttttgatcca ttttttcatc tccatttatt agcttgataa cagtatagca aaattctaca
     5281 aaaaaaactt ctatagctga ctaattaaaa actctttaaa ttactgcttt tcgttggtat
     5341 aatggaaagt gctgattata tgaaaagagg agacgctatg gatataaaga aaatgaaaga
     5401 acgccaagag cagattcgta atttttcgat catcgctcat atcgaccatg ggaaatctac
     5461 attggccgat cgtattttag aaatgacgca cactgtgacg tctcgcgaaa tgcaagatca
     5521 gctgttagac tcgatggatt tggaacgtga acgtggaatc acgattaaat taaatgctgt
     5581 agaactcaat tacacagcga aaaatggaga aacatatatc tttcacttga ttgacacccc
     5641 agggcacgtc gacttcacct atgaagtctc tcgaagcctt gctgcctgtg agggtgccgt
     5701 tttagtggtc gatgcagcac aaggaatcga agcacaaaca ttggccaatg tttatttagc
     5761 gttagacaat gatttggaga tcctgccagt catcaataaa atcgacctac ccgcagcaga
     5821 tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga atcgatgcta gtgaagctgt
     5881 attagccagt gccaaatcag ggattgggat tgaagatatt ctcgagcaga tcgtggaata
     5941 tgttcctgca ccaagtggag acttagatgc accgctaaaa gctttgatct ttgactctat
     6001 ttatgatagt taccgcggtg ttgttctaaa tgtccggatc acggacggta tggtcaaacc
     6061 cggagacaaa atcaaactga tgagtaatgg caaaacattt gatgttacag aggtcggggt
     6121 cttctcgcca aaagcagttg ctagagactt tttgatggtc ggtgatgtcg gttatatcac
     6181 agcaagtatc aaaactgtcc aagatacacg agtaggggat acagtaacgt tagcagacaa
     6241 tcctgccgct gaagctttac caggttaccg aaaaatgaat ccgatggttt attgtggtct
     6301 ttatccaatt gatacttctc gttacaacga tttgcgggaa gctttagaaa aactgcaatt
     6361 gaatgatgct gctttacaat tcgaaccaga aacctctcaa gcattaggtt ttgggttccg
     6421 ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa gaacgcttgg aacgggaatt
     6481 caatctagaa ttgatcacga ctgcaccatc cgttatttac catgttaata aaacggatgg
     6541 tacgactgct acagtagata acccggcaga ttttcctgaa cctgtaacga ttcaggatgt
     6601 agaagaacca ttcgtcaaag cacagatcat ggtcccaaat gattttgtcg gtgcagtaat
     6661 ggaactttct caacgaaaac ggggagaatt cattacgatg gattatttag atgattaccg
     6721 agtaaatgtc gtctataata ttccgctttc tgaaattgtc tttgactttt tcgacaaatt
     6781 gaaatcaagc acgaaaggct atgcctcatt ggattatgaa atgtctggtt atcaaaaaag
     6841 caagttagtg aagatggaca tcttgctgaa tggagaaaaa gtcgatgcat taagctttat
     6901 cgttcaccga gactttgcct atgaacgtgg aaaagcaatc gttgaaaaac tgaaaaaact
     6961 aatcccacgc caacaatttg aggtgccaat ccaagcagct attggacaaa aaatcgttgc
     7021 ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct aaatgttatg gtggagatgt
     7081 ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg aagaagcgga tgaaacaaat
     7141 cggctccgtc gaagttccgc aagaagcctt catggccgtt ctgaaaatgg atgagcaaga
     7201 tagtgtgaaa taacttataa tcaatcgaaa aacctattaa ataaaccttt ttgagtagat
     7261 gcaacactga ggattatctg tataaaagat tttaaactgt catttggtta tcacggcgat
     7321 actaaaatta acaaaattgg aatatttttc ttgaagattt ttagctgtca gtatatgatg
     7381 aaagcatctc ataggaggtg cttttttcat ggttgataat tcaagtggaa gggttcttaa
     7441 atctctaaga aaagagaaaa aactatctca aaaaaagtta gctgatttag caggtatttc
     7501 acaaagtact ttagttaaat atgaaaaagg gtcaagaaaa atccctaaag acgtagataa
     7561 tactctaagc aagattctga atatagaaac tctaataaaa gatgaggaag ataagattga
     7621 gattctcatc ggtaaactaa ttgcatacag agatatgaat aagttactta acaaagagtt
     7681 agctgataat ataggaatta gtgaagtatt actcagttat gtgttaaata gaaagagaaa
     7741 tccgtcaaaa gaaatgcaaa aaaaaatcga tatttttcta ttaagtaatg aaaaagaaat
     7801 tttgaaggag attaatcgtg atagtgagat atttagttta tcaaaagacg ataaaattgt
     7861 tatgggaaaa agaatacgag aagtccgaaa gaaccgggaa gagacgcttg aaaaatttgg
     7921 taaaaacttt accatctata caggcaaaaa tgttataagt agatgggaaa aaggtattaa
     7981 tattccagat attgaaaaac taatgaatat tgcctatctt ggtaaggtca ctgttcctta
     8041 ccttatgtac ggagaagatt ataagaatat acttccaaaa gatgaaagag ttagcgattt
     8101 taaaaagata aattctttta gtatgggatt aaggatgagg aaaattagga aagattatta
     8161 tctagaaagg gaggagtttg gaaagctttt ttctccttca atttcaaaat ggtccataga
     8221 taggtatgag aatggtagag atataccca
//
LOCUS       DAZKNM010000119         8178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000119 DAZKNM010000000
VERSION     DAZKNM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.120"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..523
                     /gene="rpsN"
                     /locus_tag="ACG8M9_002108"
     CDS             254..523
                     /gene="rpsN"
                     /locus_tag="ACG8M9_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0378833.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            538..717
                     /gene="rpmF"
                     /locus_tag="ACG8M9_002109"
     CDS             538..717
                     /gene="rpmF"
                     /locus_tag="ACG8M9_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0378834.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            749..1126
                     /locus_tag="ACG8M9_002110"
     CDS             749..1126
                     /locus_tag="ACG8M9_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378835.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1147..1296
                     /gene="rpmG"
                     /locus_tag="ACG8M9_002111"
     CDS             1147..1296
                     /gene="rpmG"
                     /locus_tag="ACG8M9_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0378836.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1320..2303
                     /locus_tag="ACG8M9_002112"
     CDS             1320..2303
                     /locus_tag="ACG8M9_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0378837.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2333..2455
                     /locus_tag="ACG8M9_002113"
     CDS             2333..2455
                     /locus_tag="ACG8M9_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0378838.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2473..3996
                     /locus_tag="ACG8M9_002114"
     CDS             2473..3996
                     /locus_tag="ACG8M9_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0378839.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4020..4259
                     /locus_tag="ACG8M9_002115"
     CDS             4020..4259
                     /locus_tag="ACG8M9_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378840.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4452..4913)
                     /locus_tag="ACG8M9_002116"
     CDS             complement(4452..4913)
                     /locus_tag="ACG8M9_002116"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0378841.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5108..5326)
                     /locus_tag="ACG8M9_002117"
     CDS             complement(5108..5326)
                     /locus_tag="ACG8M9_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378842.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5444..7060
                     /locus_tag="ACG8M9_002118"
     CDS             5444..7060
                     /locus_tag="ACG8M9_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0378843.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7301..>8178)
                     /locus_tag="ACG8M9_002119"
     CDS             complement(7301..>8178)
                     /locus_tag="ACG8M9_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0378844.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT         2792 a         1265 c         1640 g         2481 t
ORIGIN      
        1 ggtatttgtg tttgattgga ctaataaaag tgaattctta tttttcagag cctaaactgc
       61 atattctttt aaatgatttg attatctcta tgtaccgata aattcgtttt caaatagtta
      121 tctattcata tgagttttat ttcatgttat ttattgttga caagagcaac tcttgttggt
      181 aaagtatcag tgtcaaaacg taacgattac gatttgaggt gtctaaatca aaataaaagg
      241 agtataaagg tatatggcaa aaaagtcgaa aattgctaaa gcaaagaaac aaatggcgat
      301 gattgaaaag tatgcagata aacgtcagga attaaaagcc gcaggagatc gcaccgctct
      361 cgctaaattg ccaagagatt caaatccgaa tcgtctgagg cttcgtgatc agacggatgg
      421 tcgtccaaga gggtatatgc gaaagttcgg tatgtcccgt attaagttta gagaacttgc
      481 ccatcaaggg ttgattcctg gagtaaaaaa agcaagttgg taaaaggagg aaatatcatg
      541 gcagtaccag caagaaaaac atctaaggcg aagaaacggt taagaagaac tcatcaaact
      601 gtagtaaaac cagaaatttc atttgacgaa gcgaatggtg attatagacg tagccatcat
      661 gtatctttaa aagggtacta taatggaaag aaagtaatta aaggagcttc aaaatagttt
      721 aatatataac ttttaggagg gaatcaaaat ggaaattatt tacccacctt tagttgaaga
      781 aggattaaag tattatcttg aaacgaccca gcaatcgcta gataaatgca ctttttatcg
      841 ttcgatggta gagagaggaa ttattactga aacgggttta ccgacccaac aggcaattga
      901 aaatggatta gtcaaagatt actatgagga ccaaggctta tcatttgatg attttttaag
      961 aatctatcca atttttgaag agtatgacga ggagttattt caatgtatcg atggttattg
     1021 ggagatacct gttgatatga aagaaaattt agtttcacaa ttggaatctg gcgagttaac
     1081 ttttgaagat gcacaacaaa ttcaagcata tcttgaagat cgataagtat tgaaggagga
     1141 agatttatgc gtcaaaatat tattttggaa tgtgtcgaaa ctggagaacg tctgtatctc
     1201 accagcaaaa ataaacggaa taaccctgaa cggttagaat taaaaaagta ttcgccaaaa
     1261 ttgcgcagac gggcaatttt taaagaagtg aaatagctaa gtggtaaaaa ggtgacaaaa
     1321 tgggcatacc agttacaatc gtttctgggt ttttaggggc tggaaaaaca acgctaatca
     1381 atcaagcctt gcaagtctca ccctacccca gggaagaaat tattataatt gaaaatgagt
     1441 ttggtgaagt tgggattgat cacaaattat tagttcatag cgaggaacgg gtgttacaac
     1501 taaataatgg atgtatgtgt tgcagtcttc gcagtgattt attagcaata ttgatttctt
     1561 tactggaaac aatagaagaa catcaacaac caatttcaca aatcatcatt gaaactacag
     1621 gtatagcaga tccccaacca atcattcaaa cacttttaac agcgcctcaa ctaagaggaa
     1681 gagtatatat cgatagttta ttgactgtta ttgatagtga cagttttgaa cttattgaaa
     1741 gagaaccaca agcccttaag caacttgtca tggcagatcg catttttgtg tctagaaaac
     1801 aaaacttgaa tttatctgca ctaaaaaaat tacagagaga aatcaaaagt attaaccctt
     1861 tatccgatat tcgaatgttc acacatagtg aaacgattca aaaaaaggat ttttataatt
     1921 tgaaaaaatt taatcatccc ttagtttttg aaagtgattc atcagaaaat cactataatg
     1981 gcaaatacta tcatcaacac catcaagtac atgagtttaa atcaattctc ttaacttcag
     2041 atcaagacat aaaaaaagat tttttactcc aatggattga ctggctgatt ttaaccaacc
     2101 aagggagcct gtatcgtttt aaaggattga ttgctttaca agagcaggat tttatagttg
     2161 ccatgcaaga ggtaaacgaa caggttaatt ttcaatacac cactcgacaa aaagaccaaa
     2221 ccactattat tctaatcgga aaaatgttgg atgaagaaag gatcaaacag agttttgata
     2281 aattactggc ggaaagcaaa taatgataag tagtgattgg gggtaatttc taatggagaa
     2341 aacggatata tctagtgcat atcgacgact taaaagcccg aatattaaaa ctagaaaaag
     2401 ggcattaaaa attatcaaag aacataaacg aaataaaatg aagaaattag cataattaag
     2461 gaaggaaaaa gaatgaagaa aatcagtgta ggacttattg ggatagcagc tctaggatta
     2521 ttgggagctt gctccagcac taatgatgca aaagtatcga atgataaaga tggaaaatta
     2581 gaaattgtaa caacattcta tccaatgtat gatttcacca aaaacatcgt cggcgatgaa
     2641 gcgaacgtgg atttaatggt tccagcggga tcagaacctc atgactatga gccctcagct
     2701 aaggatatgg caaaagctca tgatgctgat gtttttgtat atcataacga aaacatggaa
     2761 tcatgggtac caaaagctaa agaaagttgg aagaaagctg ggcctaatgt agtagaagga
     2821 acaaaagaca tgattttatt gccaggtagt gaagaagaag atcatgatca cggagaagaa
     2881 gatcaccatc atgaactaga tcctcatacg tgggtttcac ccaaaatggc aattaaggaa
     2941 gtttcaaata tcaaggatca gttagttaaa ttgtatccga aaaaagcaaa agtatttgag
     3001 acaaatgctg aaaagtattt gactaaactg aaacggttgg atgctgacta taccactagt
     3061 cttaaagaag cgaaacaaaa aagttttgtg acgcaacacg cagcctttgg atatttagct
     3121 ttggattatg gattgaaaca agttcctatt gctggactaa gtcctgaaga agagccatct
     3181 tctggtcgat tagctgaatt aaaagagtat gttaaaaaaa ataagatcaa ttatatttac
     3241 tttgaaaaga atgctaatga taaaattgca aaaactttgg ccaacgaagc tgggataaaa
     3301 ttggaagtac tgaatcctct tgaaagtctt acaaaagaac aaatggataa tggtgaagat
     3361 tatgtttcag taatggaaga caatctaaaa gcgttagaaa aaacaactat ggttgctggt
     3421 gaagaggtag ttccagaaaa agaagcgaag gatgaaaaga cagttgctaa tgggtacttt
     3481 aaagatgccg atgtgaagga tcgcgaattg agtgattata caggcgaatg gcaatctgtt
     3541 tatccgttgt taaaagatgg aattttggat gaagttttcg attacaaagc aaaaatcaat
     3601 aaagacatga cagctgccga gtacaaagat tattatacta ctggctataa gacagatatt
     3661 gatactatca acattaaaga caacaccatt gattttgtag tcaacggtga acatcatcaa
     3721 tacacataca aatatgttgg atacaaaatt ttgaattatg aaaaaggaaa ccgtggtgtt
     3781 cgttttaatt ttgaaactga tgatgcaggt gctggtcgtt ttaaatatat tcaatttagt
     3841 gatcatggaa ttgcaccaag taaggcagag catttccata ttttctttgg tggagaaagt
     3901 caagaaaaac tctataatga attgcataat tggcctactt tctatccagc ttcattgtct
     3961 aaacatgaaa ttgctcaaga aatgatggct cattaaaatt caatttttgg aggaaataca
     4021 tggtaagaaa agtattattg tctatggatt ccaagaatat tgatgcttat cggcgttatc
     4081 gatttgcaaa gcaaatgaga ttaaatggga gtacattagc tgatattgct gcaactttac
     4141 atgttagtat tgacacagtt catagagtat taaaacggga tcctgattgt tgttttatca
     4201 aggtcaatac tggaagaagt agacgtttag caaaacggca taatagcttg gccaattaat
     4261 tgataatacc agagtagatc atttttccct tatttggatt tgatctgctt tttattatgt
     4321 cttgaattgt caaatcaagc gcaacacttt atcaaagaag acttcataag gtgttctcca
     4381 atctaaacac tttctaggtc tgttattcag tttttcaatc atctgattta tcgttgagtt
     4441 atctaccgta gctatctctt tcgattttgg caagtattct cgaatgagtc tattcgtatt
     4501 ttaatttgtt cattgttgcc aagggcatga ggatctggaa aataaaaggg catattattc
     4561 aatgcttctg taactgcgaa atgattggag aattcttttc ctctatctgg agtgatcgtt
     4621 ttgaccgcct catttggaat actgcttaaa agttcaatca ttttctcgct cacataagcg
     4681 gctttctttc tggggacctt ctttcctgct aaaaggaagc atgattttct gtctgtcaaa
     4741 gtcactaaac aagtaccacc agttttgcca gctacaatat cggctttcca atgaccaaaa
     4801 tcacttctat catctgcgga ttttggacgg ccatggatcg aatgagtaag gcaaatcttt
     4861 ccacgagact cgacatgatt ttttgtatgt cttgtttttc ctcgatggtg caatgaacgg
     4921 atcaggcctt tattgccgtg tgagaggggt tctgtatcga aaagatcatt gcaaattgca
     4981 cgttagatcg tgataaaact aattgaaaag tccatatgtt catgcttcaa acgattcgag
     5041 atttcctctg gcgaccactg aaaatcgaca aataaatgac gaacaattga ccaaactcac
     5101 ggatcattta gaatgtgata acgtccgcat ttttttcttc gataagcata atttttttcc
     5161 gctttggaag gtgaatagtt tttttcttgt ttcttctcag ttcatgtaaa atagtaaacg
     5221 ggctatgctg aatgtcgttg ccaattatac taatagaaaa tccttcttcg tgaagcagaa
     5281 agattctttc tcgttcgagt atagtaagat gacgatactg gctcataatt attttccttc
     5341 taggagtgtt ctcgacaaac ttatactagt tggatatcga ttatgactca tttttatgtg
     5401 ttgcacttaa agtgtaaatt caaggtatag aaagaggttc cctatgaaaa aaatacctat
     5461 tacaatctta actggctatc ttggttcagg aaaaacgata cttctaaatc atattctgta
     5521 cggtgatcac ggaaaggaaa ttgctgtcat tgtaaatgac ataggggaag taaatgttga
     5581 tggggtatta gtcgacaaga aaggttttaa aagaactgga gaaaaacttg tttcgatgcc
     5641 gaatggatgc atttgttgta cattacgcga agatttgatt gttgaactag atcaattagt
     5701 gcaattttct gacttgaatc aaattgttat tgaagcttca ggaatcagca aaccaattcc
     5761 aattattcaa gcaattatat tagctaaaaa ccctttagac attgatttga cccaacgatt
     5821 agagattggt gccattatta tagtagtgga tgtaaaacgt ttttgggata atttcgaaac
     5881 gggtgaaaag attcaggaaa gaaaagctga aggtaatgta gttgaagaag aacaggatat
     5941 tcgtggcttg ttaattgatc aattcgagtg ttgtaacata ttacttttaa ataatagtga
     6001 cttagtgtca accaagacga tttacaaaat taacggtcta attgctaagt tacagtccaa
     6061 tgccaaaata attaggacaa ttaaaggtga agtagactta tcgaaaattc taaatacaaa
     6121 tctttttaat atagatgaga cttcctattc agtgggctgg ttcaaagagt tagagcttgg
     6181 ctatcaaaat catattcctg aaacagaaga atatggtatc tcaagtttta tctatcgaaa
     6241 tcggttactc cttgacgcac aaaaatttgc ccatcttacg gataaaaatt ttcctggcaa
     6301 tattacaaga gcaaaagggt ttctttggtt tgcccaacat agtgattttt cgacgatctt
     6361 tgaaacatcg ggtagctata aaagtattga atcattaaat tattgggtag ctagtttacc
     6421 taaaattgag tgtcaaatga ttatacaaaa aaatcccgaa ttgaaggaag cctggcatcc
     6481 gttgtttggt gaacgcatta ataaactagt ttttattggt atgaagatgg atcaacaatg
     6541 tattaaggaa caacttgagg gttgtttaac tgattctctt aatgcagata atttccgaaa
     6601 aatttcacca tttccatgga aaaagtctaa tatgtttagc cagatatcgt taatgaaatt
     6661 tttaagaaaa gctggtgtca agataggcga tgtcataatt attggcattt tgattattac
     6721 ctcttttatt cccttatttg tattctctat agaaaaggag aatgaaacaa ctgaagatgc
     6781 tatcaaatat gccatcgttc gaattgatgg caaggaaatg gatcgcttcg atttggataa
     6841 gatcaatcat aaactcgtga attatcatcc tgcgaaggga caatataata ttgttgagat
     6901 taaagatgga agaattcgag ttaaagaaga taatagtcct gatcaaatcg cggttagaac
     6961 tggatggatc tcaaaacccg gtcaaacgag tatttgttta ccccataagt tagttattag
     7021 tattgagaaa aaagaaagca cagattacta catttactaa tctggaaata gatgaggagg
     7081 tttttaataa gaaaaagata ttattattaa taatagcagg ggctttatta agtgtgttaa
     7141 gcgcttgtgg acaagaaaaa gaagcggtga caacaggcac aagtatcttt aacgggacct
     7201 gcaatggttt agttaacagt cggttagatt acttattttg gctcttcgtc aatgagtgtt
     7261 ggtgaagaaa ttcaatagaa taatcgagat actcacacgg ctagacagct atcttgtcta
     7321 gccgtgttgt tgttctggct ttagtcagtt gggtagtatt cttagttttc ttaaaatatt
     7381 ttcctcgata taaaaagatt cgttctcggt agttttgaaa gtttctatag ccataggcaa
     7441 ttcgttttag tactttgata tggttattca aacattctat agggccgttg gaatagggaa
     7501 cgcgcaacgc tcgtttgatt tctgtttggt atttcttgaa ggtcgtgaac actggttggt
     7561 agtgctctgg caactccttg tagtcttctt ttaggagcgc gacgaattca ggaacatctt
     7621 gtcttctgat aacggaaaga aaatcctggt agacttcgta gcctcgcttt aaggaatcat
     7681 cataagataa tagccgatcg actatctctg cttctgtcag atgatctcga aatgacggac
     7741 gccagtagcg atgctgacct ttcaattccc acgcattctt ttgaagtagt ttccagtatt
     7801 tctttaagtg cttcgaccga cgatcccctt ttttaaaggt cttcattact tgaattcggt
     7861 ggttggtaaa agcccgacca atgtgttggc taatatgaaa gcgatcggcg acgattcggg
     7921 cattcggaaa acactctttc accaatgtgc gatagggtgt atagaaatct gaaaccacta
     7981 gacgcacttg ttcacggact tttatagggt agcgcaagaa atagtcgcgt aaaggattga
     8041 gttgccgatt ttccacaata tcaatcagtt gatgggtctt gccgtccatc atgacaaagc
     8101 tcatggctcc cgagacattt ttcactgatt tgaattcatc gaaacacacc acttctggta
     8161 acagctggct caaagaaa
//
LOCUS       DAZKNM010000120         8160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000120 DAZKNM010000000
VERSION     DAZKNM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.122"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..639
                     /locus_tag="ACG8M9_002120"
     CDS             31..639
                     /locus_tag="ACG8M9_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0378845.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            807..2444
                     /locus_tag="ACG8M9_002121"
     CDS             807..2444
                     /locus_tag="ACG8M9_002121"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0378846.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2516..3628)
                     /locus_tag="ACG8M9_002122"
     CDS             complement(2516..3628)
                     /locus_tag="ACG8M9_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0378847.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3703..5076)
                     /locus_tag="ACG8M9_002123"
     CDS             complement(3703..5076)
                     /locus_tag="ACG8M9_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0378848.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5297..5914
                     /locus_tag="ACG8M9_002124"
     CDS             5297..5914
                     /locus_tag="ACG8M9_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0378849.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6012..6467
                     /gene="argR"
                     /locus_tag="ACG8M9_002125"
     CDS             6012..6467
                     /gene="argR"
                     /locus_tag="ACG8M9_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0378850.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6485..7987
                     /locus_tag="ACG8M9_002126"
     CDS             6485..7987
                     /locus_tag="ACG8M9_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0378851.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            8030..8155
                     /locus_tag="ACG8M9_002127"
     CDS             8030..8155
                     /locus_tag="ACG8M9_002127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378852.1"
                     /translation="MVWLNRVLVGRPAKGGKNGNEYGYFFNSIGLWGQLSGIYVS"
BASE COUNT         2798 a         1545 c         1693 g         2124 t
ORIGIN      
        1 ataaaaaacg gagaaaaaaa ggagtttaat atgaattcat ttaagaaaat tgttttaggt
       61 acaacttttg ctgctggtgc tactgcaatg tttgtaggaa ctacaaatgc tcacgctgat
      121 gaagtttata cagtaaaatc aggtgattca ttatctaaga tctcacaaaa atttgcagga
      181 gacaactcaa tgatcgatgc aattgctgaa aaaaattcga tcgcaaacat caaccgcatt
      241 tatgttggtg aacaattaac aatcccaact tcaaatgatt cttcagcaac aacagaaaac
      301 aaaacaacag aaaatacagc atctacaact gaaacagcaa cgcaagaaca tacatacgtt
      361 gcacctgttg aaacagttga agtagctcct gctgccccag ctgctgcaac agcgccaact
      421 tcaagctctg caaaagaatg gatcgcacaa aaagaatcag gcggttcata cacagctact
      481 aacggacgtt acatcggccg ttatcaatta gatgcttcat acttaaacgg cgactattca
      541 gctgccaatc aagaacgtgt agcagaacaa tatgtaacat ctcgttatgg ttcatgggat
      601 gctgctaaaa cattctggtt agcaaacggc tggtactaag ccgataaaag gaaaatgatg
      661 aaaccgagaa acttgtttat tcaagttctc ggtttttttg tgtgaaaaat aactcgtttg
      721 cttcccacag cgcagcagta caaataaaaa ctcgaataat gtataataaa gaaaacgttt
      781 ttttgtttag ggaatgagga ggagccatga gtacaaaaga gagtctcgaa ttaaacgaaa
      841 tcacaagtgg aaatccactg ttatccagta ttcgatcgat tgtagaaacg acattttacg
      901 gtaataatgt tcatgaagtt tttgatagga agacagctta tcaactagca aaagggagtc
      961 caggaacgat catcacagat ttaacaatct cacatgctga agaactagat ttaccagctg
     1021 atgttaggac actagtattc aatgatggaa gcattgtggg aagaacagca agtgctcggc
     1081 ggatctttga ggacttagac aaggaacaaa gcaaatacga gaaaatttta agagaagctg
     1141 tgtatcaaag tcgcaagcgc cagttttacc atacaaaagt aatcgttggc ctatctgaag
     1201 aattttctgt gcaaagtcat ttgctgattc cagaaggatt tgagaacaat ttgtattctt
     1261 atttgctgaa ttttcagcca gcgaattcga agatcttaga gacttatcaa aagtctgttt
     1321 cctttgatga gggagatctt tatatctttt gtgatcccga atggacacat gaaacatttc
     1381 caaatggact cattttgttt gatgctctcc ataatacagc ggctgtgcta ggattacgtt
     1441 actttggaga attaaaaaaa gcgactttga cgcttgcttg ggcattggga aatcgcaatg
     1501 gctttattgc ctgccacggt gggatgaaac aatatgctag taatgaccag acctatacga
     1561 tggctgtatt tgggttgtcc ggttctggaa aatcaacgat taccttagca aaacatacgg
     1621 aaggtcactc tgtttcgatc ttgcatgacg atgctttcgt tatttctcga aaaaatggtt
     1681 ttgctactgc cttggaacct tcttactttg ataaaacaca agactatccg tctgatgatc
     1741 cggccatttc ttctttcctt acttgtcaga atgtgggcgt gacatgtaca agtaaaggag
     1801 aaaaagtcct agtaacagga gatatacgaa acggaaatgg gcggacagtc aaatcacgtt
     1861 atgcgacacc cgaccgagtc gatcatctta gcgaaaagat cgatgctgtt ttttggatca
     1921 tgaaagatga ttcgcttccg ccaattatca agatcaatga cccagtttta gcagcgtctt
     1981 ttggtgcaac attagcaaca aaacgatcag acgcggaaaa tatcactaca aaaagacagc
     2041 tagatgagct agtcattgaa ccatttgcta atcctttccg tgtctatcct cttagggaag
     2101 actatcaagc ttttcgagag ttgtttttga aacagcaaac agcctgctat gtcctgaata
     2161 ctggatattt cgatgataaa aaaatcaaac ctgaaacgac attgtcaatc gttgaaagaa
     2221 tcgtgcaaca aacggcagaa ttcaagtcgt ttggtccatt tgaagacatc cactatttac
     2281 ctataccaga ttttcctgtt tcttttgagg atacgtccta taaaaatctg gtgaaacaac
     2341 gacttgatat tcgtttacga ttcattgaag ctcagatagg aaaagatctc ttgccagatg
     2401 aagcaaaaga aagtttggag aatttgattc agaaattgaa ctaaaataac agctttttca
     2461 agaagtaatc catgcgcaat atcgctcggg atccttactt gaaaaagctg tttttctatg
     2521 atggattttt cgtaatatag gtggagatgt ccttttgata atcttccgta ggccaatgac
     2581 cagtatttaa gaagttggct aatagatagc cgatataagg acctgtggtc aagccagaag
     2641 agcctagacc gctagcagcc agtaagcttg gatcatctgg cagtacgccg aaaaatgggg
     2701 aaaaatctga cgtataagca cgggtaccta cacggaactc gtatggccag ttttttatct
     2761 gatctggatc agtcaagaac tgttctgtac tcttagtcaa tcccttataa gcttcgctag
     2821 taggagccaa atcccagcct tgatcgtttt catgggtagc acctaaaagg atctttccat
     2881 ccatgaaagg aatgaaatca gcttctccgt caagcatagc aacaggccag tcttgactca
     2941 tcttataaga tgtctgaaat gctaagagct gccctttttg cggacgtata tctgcagcat
     3001 agcctaattc ttgtaataaa ggcttaagtg ccgggccagg tgtcaaaatt acacgatcag
     3061 ctgtgtattc tccaaacggt cccttgatga gccaagaatc gctcttctta tgcaaagtga
     3121 cttttgcctg gatctgctcc atttgttttt gtgctaaacg ctgaccaaga tgttctaaat
     3181 atgcttttcc atccagacga ccaccaccac tgatacgcag tgaaactgct ggtttcaata
     3241 acggaagtgc ttgagatgtc tgttcaggtg aaagccattc gatgtttcca atttctggcg
     3301 cttcttcttt tcgtttctcg gcaagaacag ccaattcagc taaatctttt tcaggtcgaa
     3361 gcaaaagtgt ccctgattgt gcgtaaatcg tttgatcaag ttggaaatct tttataagtt
     3421 tagggaaaaa agcagcgccg tctttagcca gacgatacca ttgtttgttt ctgcgtttcg
     3481 acaaccaagg ggagataatc ccagcgctag ctttagttgc ctgtcctgtc ccttcatcaa
     3541 ataaagcaac attgaatttt gatgtatcta agtaactggc tagggtcata ccaatgatcc
     3601 cgccaccgat aatggcaatt ttttccataa aaaagcctcc ttttacaaac aaccattttc
     3661 acacaaaaaa acggcagatg caacacctgc cgtatatgga atctacgctt ttttagcaaa
     3721 gaacagataa taggcgaatc gagcaaagct tacgagaagc aacagcaaca agacgcctaa
     3781 tgctgctttt gatcctaatg tagtagacac ttcatagtta tgggaagtat gatgctggac
     3841 gtaattgata atgaccgcta ctaatgaagt agccacagcg ccagaaaact gctgcaaggt
     3901 attgaacatt gcgttcccgt ctccttgtag ttcttcattc agtgcattca ttccggtggt
     3961 catcatattg ctgtaagata atccgatacc gatcatataa aacacatgac aagcaatcaa
     4021 tacacccact gaagcgtgtc ctatgagtag gaataatgct agccaaccaa tcaaagaaag
     4081 aatcagccca gcaaaaattg gcttcttcgg accatattga tccagcattt tcccggaaaa
     4141 aggagaaagc aaagcaccaa ccgctgcgcc gggaagcatc gctaatccag cgacaaaagg
     4201 actacttcct tgaacgatct gaaggaaatt cggcaagaca aaagatacac caagtaataa
     4261 aaactgataa actaagaagc caatcaaaaa caagcggaat gttttatttg aaagaataga
     4321 cagtcggatc agtggatgct ccactttttt acttcgatag taaaaaagga accagccaat
     4381 accgccgatg attaaagcaa tccagctgga aactgttccc aacgtactca aaaatgttag
     4441 acttccacta aacaaaagac aaattcctaa aacgcttatc caatccaaat aaccactttt
     4501 ttgtactgcc atctcgggaa tagcaaacca gccgataatc aaagaaagaa acaaaacggg
     4561 cacaagaaac aggaagatat gattccatgt aaaatgtgaa gtcataatgc ctccataagt
     4621 ggggccaatt gctggagcaa tggccgttgt caaagtaccg acacccatca tcgcgcctcg
     4681 tcgattgagg ggggcaaatg tcaagataat atggaacatc aacggaagag cgatacctgt
     4741 cgctgcacct tggagcaatc gaccaaacaa taggaccatg aaggtaggag agaagaaatc
     4801 gatcattata cctgccaaga aaagcagatt tgaagctaaa aagagttttt tgagactaaa
     4861 attgcgattc aaataagtag acagcggaac aatgatcgaa atcatcaaaa gataaatcgt
     4921 tgttacccat tggacttgtg cagtcgttaa atcaaactgt ctgataagag ttgggaaagt
     4981 tacattcatg gccgtctcga tcaatacgcc cgcaaaagac atcatccctg ttgcaattac
     5041 tgcaggtact aaaagcagct gtttttgttt tgtcatttac atcacctcta aaaaatttcg
     5101 taaaaaaaga gagtgatttc tattataaaa atagaaaccg cctctctctc gacacgccga
     5161 aacgtgttat ctgtacgatt tatttacttc acttattcta gcacaaactg aaaaatagtg
     5221 tcaacacgat tagccaagtc actcaaaaag aggtatgata ataaataact aaatggaatt
     5281 tcgcggagga agaaaaatgg aaaaaattga atggcgtaaa gacaagagct atagtatgaa
     5341 aaaaagcccg caaatactag aggttcctaa aaaatcattc tttagcatca gagggatcgg
     5401 cgacccgaat caggaagact ttaaacgaag agtagcctgt ctatatgcag tcagctatgc
     5461 aatacgcatg tcgcctaaaa aaaattggct gattcctgat tatcagatgt atactgtcta
     5521 tccactggaa ggacaatggg gactgcaaga aaagtatttg catgaaccag tgatgaaaaa
     5581 agaacatttt tcttatcaat tgatgatccg acagcctgac tttgttacag aagcgatagc
     5641 acaagaagcc attcagcgtt ctagcaaact tccggaagac ttgaaagaac aagtagtatt
     5701 caaaaagatg gaagaaggtc tttgtgccca aatcttacac gtcgggccct atgccgaaga
     5761 accagcaacg tttgagaaat tagaagcatt catcgcaaaa gaaggctatg aaagagcttc
     5821 taaagaacac aaagagattt acatcagcga tccaagaaaa tcagcacctg aaaaaatgaa
     5881 gacgattttg cgtgttaaaa tagaaaaagg ttagcaacct gaatgaaaac aaaaaataaa
     5941 agtaaaaaat accaaaatat gaagaaatat tacttatctt ttgctataat caacattgag
     6001 aggtgtctaa tatgaaaaag aaacagcgac aagcactgat ccgccagatc attacagaac
     6061 aacctattgg gacacaggaa gagctgcttg cgcggctgca tgaagcaggc gccgatgtga
     6121 ctcaagcaac gatttctcga gacattcgag aaatgaagtt agtaaaaagt caaaatgaaa
     6181 acaaaatagt acgctataca ttatttaacc agccatctgt ttctttaaat gaagaacgtt
     6241 tgcgtacagc aattagacgc gaggttttgc gtatccagtc tgttcagttc atggttatcg
     6301 tattgactga acgaaatggt gccgatgtgg taacgaattg gctagatgaa gtggcttacc
     6361 ctgaagttgt cggaacgatg gccggtgtag atacattcat tattatttgc cgctcagaag
     6421 aagaagcaca gcgatttgca gaaaagctgg aaaaaatgag ggaataaatg aggaggactt
     6481 ttatatgaaa gatatgaaag cgctgcaaaa tggatcggat attcgaggaa tcgcattacc
     6541 agttgaagga tatgaagtaa acttgacgac agacatggta aaaaagatcg gctggggact
     6601 agcaaactgg ataaagaaaa agaagaaatc aaccgatcca aataaaaaat ggacgattgg
     6661 gattggtcat gacagtagga taagcggacc agcactgaaa gaggcgttga tccaaagtct
     6721 gaaagaacaa gggatacatg tcatcgattt cgaattagct actacgcctg ctatgtttat
     6781 gtccactcaa tttgaacagt tctcgtgcga tgctgcggtt atgctgactg ctagccatct
     6841 tccctattat tataatggaa taaaattctt tacctcagaa ggaggagcag aaaaagaaga
     6901 tattgctttt atcctatcac agcagcaaaa aataaaaagc gaacaaactg gaagcgtaga
     6961 aaaagcagac cttttgacac cttatgcgca agatttagtc acaaaaatta aaaaggggat
     7021 tggaacacta gaaagcaaac cactaaatgg ttggaaaatc attgttgatg ccggaaatgg
     7081 tgctggtgga ttctttgctg aaaaagtatt gaatagctta ggtgcggatt caacaggttc
     7141 ccaatttttg gaaccagatg gccgatttcc taatcatatc ccaaatcctg ataataaaga
     7201 agcgatggaa agtatcaagc aggctgtttt agcaaatgaa gcagatcttg ggatcatttt
     7261 cgatacagat gtggatcgtt ctgcagttgt cagtgcttct ggaacagtga tcaatcgaaa
     7321 taatttgatc gcagtattaa gtacaatcgt attaaaagag catccaggaa caagaatcgt
     7381 gacgaattcc cctacttctt ctcatctaca aagattcatt gaagaaaaag gaggcatcca
     7441 agttcgttat atttccggct atcgaaatgt aatcaataaa gcattagaac ttaaccgtct
     7501 tgggaaagat acacagctag ccatcgaaac aagcggacac gctgcattca aggaaaacta
     7561 ttttctggat gatggtgcat atgtcatcgc aaagatcctg atgcttttgc cagaattaaa
     7621 aagaaaaggt atcacattag atgaacaaat caaagagttg aaacagcccg tagaagtaca
     7681 agaaatccga ttaaaattgt tcagcgatga accacgcgaa caaggaaaca agatattaga
     7741 agaaatgaag cgactgttgg aagaagaaaa agggatgaac ttcgacccgg aaaatgaaga
     7801 aggtattcga tatgatctag aagagccata cggaaacggc tggtttttga tccgtttaag
     7861 cttacatgag ccgttactag tattacagat agaaaatgat gaattcggaa agaacaagtt
     7921 ggcgttagta aaattagcaa aattattcgg acatttttct gatcttgaat cattaacagc
     7981 agaataaacc agtctttttg aaaggctagt tgcttggacg gcaaaaaaag tggtatggtt
     8041 aaatcgagtg ctggtaggga gaccagcaaa aggaggaaaa aatggtaacg aatatggtta
     8101 tttttttaac agtattggtt tatggggtca actttctggt atatatgtat cttaaaatca
//
LOCUS       DAZKNM010000121         8048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000121 DAZKNM010000000
VERSION     DAZKNM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.123"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACG8M9_002128"
     CDS             complement(17..538)
                     /locus_tag="ACG8M9_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0378853.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="ACG8M9_002129"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="ACG8M9_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0378854.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /gene="cdaA"
                     /locus_tag="ACG8M9_002130"
     CDS             4618..5505
                     /gene="cdaA"
                     /locus_tag="ACG8M9_002130"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0378855.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="ACG8M9_002131"
     CDS             5502..6671
                     /locus_tag="ACG8M9_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0378856.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..>8048
                     /gene="glmM"
                     /locus_tag="ACG8M9_002132"
     CDS             6695..>8048
                     /gene="glmM"
                     /locus_tag="ACG8M9_002132"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0378857.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2580 a         1489 c         1827 g         2152 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gattaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggatt
//
LOCUS       DAZKNM010000122         8030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000122 DAZKNM010000000
VERSION     DAZKNM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.124"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..2826
                     /locus_tag="ACG8M9_002133"
     CDS             49..2826
                     /locus_tag="ACG8M9_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0378858.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2907..3326
                     /locus_tag="ACG8M9_002134"
     CDS             2907..3326
                     /locus_tag="ACG8M9_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0378859.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3338..3808
                     /locus_tag="ACG8M9_002135"
     CDS             3338..3808
                     /locus_tag="ACG8M9_002135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0378860.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3825..4604
                     /locus_tag="ACG8M9_002136"
     CDS             3825..4604
                     /locus_tag="ACG8M9_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0378861.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4591..5412
                     /locus_tag="ACG8M9_002137"
     CDS             4591..5412
                     /locus_tag="ACG8M9_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0378862.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5429..6478
                     /locus_tag="ACG8M9_002138"
     CDS             5429..6478
                     /locus_tag="ACG8M9_002138"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0378863.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6491..7495
                     /locus_tag="ACG8M9_002139"
     CDS             6491..7495
                     /locus_tag="ACG8M9_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0378864.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2660 a         1354 c         1685 g         2331 t
ORIGIN      
        1 taaagatttt attgacaaaa tgaatccgtt ttctatgatg gaggaggggt gaataagttg
       61 ttgaagaagg aaattttatg ttacttagag aaccagacag cctttttcga tttagaacat
      121 atgaacgagg tctttacagc acaaaggtta gctgatcatt ttggggtaaa aagaaacaca
      181 gtcagtcatt atttgaacca gttaaatgat gagcaactac tagtcaaaat caattctcga
      241 ccagtgattt atttccacaa agaagctttt gaaaaacaat tttttaagtt acgcacgaat
      301 ttttattttt ctatcgatga tctcaaagag gaacagcctt ttttcgcgca accaaaagac
      361 ttattctctt ttatgatcgg atacgacgcc agtttgaaag aaagtatcga acaaatcaaa
      421 acagctcttt actatccaga tggcggatta ccacttttga ttacaggcga aagtggcaca
      481 ggtaaaagtt atttagttaa tttggtctat cagtattgtc tgcttcatga tctgctagaa
      541 gattctgcac cattcatcac ttttaattgt gcacaatatg cagataatcc tgaacttttg
      601 actagcaatt tgtttggaca tgttaaggga gcgtacactg gtgcagaaga aaataaaaaa
      661 ggggcttttg aagcagcaga tggtgggatc ttgtttttag acgaggtaca tcgcttaaca
      721 ccagaaggtc aggaaaaact gttcacctat cttgatcaag gtgtgattta tcgtatggga
      781 gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt ttgctacgac tgaagagatt
      841 actagtcatt tcttaacaac tttcattcga agaataccta tccgaatcga actacctgca
      901 cttaatcagc gtagtcgtaa tgaacggcta gaactgattt actctttttt tctcgaagaa
      961 caaagaaaaa tggggagccc tttgtcagtc agtggaccag ctttgtcact tttaacaagt
     1021 cagaaattat ccggaaacat aggagagctg aaaaatatcg tcaaagtttc agcagcaaaa
     1081 gcatatgctg aacagcgtga acagcctgaa atccatgtca cgatccatca tttgcaaaaa
     1141 gaactacttg cgcagcctat tactaaaaat acagcacagc aaggaatcta tattacgcaa
     1201 gaccagacct tagagcaatt aatggagaag caacagcccg aacagcagcg aatcgttaaa
     1261 agttttgagc aaatcctaat tgatttcaag aaaaataact gtctgctata ttcttgcgaa
     1321 ggaaaattaa agcaagaagt gattcaatta tttgattttt tgctgtttga gaccagtaga
     1381 caagaaaaac acgagctgct ggtttattgg acacaatcta tccgagagac ctttcgacaa
     1441 atggaaactg cctatcaaat caaattcgat ggaaacagtg tctatgcctt gagttactat
     1501 ctctatcagc gaagcactat caaatggtta ccggaagaac atgatattat ccaactaatc
     1561 aaagaattag aacaacaagt ttctcagtct tatccatcta gttatcacta tgtccaacga
     1621 attctggaat taagtaagat caaattagat attgaagaca caagtatgga tcggatttta
     1681 ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa aacgactgcc aaaagcgatg
     1741 attgctgctc acggttatgc aactgctagc agtatggcaa atgtagtaaa tcgtttatta
     1801 caagaggatc tatttgaatc atttgatatg ccactagatg tcacaccaca gcagattgct
     1861 tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa atggattgat catattagta
     1921 gacatgggat ctctgaaaga aatccatcag ttttttaaaa aacagttatc cgtaccgctt
     1981 ttgattttga ataatgtgac gacaccttta gcaatcacag taggggaatg cctgcaaaaa
     2041 aatgtatcat tagaagagat agcagaagag actgttcgac agatccagcc agaatggcga
     2101 ctgctctatc cagaggaaaa caaacccaaa gcactcatta ctacttgctt cactggaatc
     2161 ggtacggcgg tccacttaag tgaactgcta gaaaaaagct tacctacggc ttgtcagttg
     2221 aaaatcatcc catatgaata tcagcaatta aaagataaga aaaacagtga cccattattt
     2281 tccatatacg aagtcgtagg aatgattggc accacagatc ctgatatcac caatatcgac
     2341 tatctttcat tagaagacct catctcgggg gaaaagatgt ctgtactagc tgaatggttg
     2401 gacactacta tgaatgcttc agagaaggaa atattcaatc agcgcatcat tcgtaatttt
     2461 tcattggaaa aagtactgga ttccgtgact attctagata cagagaaggt gatgggagaa
     2521 atcgatcatt ttatgagaga attagaggtg cagttgaatc gaagcatcag caatcccaaa
     2581 aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac gattgatccg acaaatgcca
     2641 atcgaaacct atcaaggatt agagaaatgg aagcagtgtc aaaaagaagg gttatctgct
     2701 attaaatctg cgtttagtgt cattgaagag cattatagtg tcatgatccc caattctgag
     2761 attgcctata tctatgatat tttgagtgaa gatacagagt ttctctcgat agaagaagaa
     2821 ttttgaacat aagcaagttg ttgccactaa ttttggcatg caacttgctt ttttagtatg
     2881 tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat tatcttagct tcccatggaa
     2941 aatttgcttc agggatatta gattcattag agctgatcta tgggaaaaat cattctatta
     3001 ctgtattcga ttgctatact gatgagaatt ttgatttagc ggaaacagta aggcagcttt
     3061 ttatgaggta caaagataaa gaaatcattg ttctgacgga tctttttggc ggaagtgtca
     3121 acaacgaatt cttacaatat atcaatcggg atcatgtcta tctcgttgcc ggattgaatt
     3181 tgccactatt gattgagcta gtatctcggt tggacgctga agtagagaca actgttttga
     3241 tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt ttgtaacgag cggctcaatc
     3301 agaaaataga agaagacgaa ttttaggagg aagaaaaatg attgtattga caagagtcga
     3361 ccatcggtta ttacacggac aagtagcttt ttcatggaca caaacaatag gcgcagactg
     3421 tattttgatt gctaatgatg atgtacctac gaatgagata cgaaagacaa cgatcaaatt
     3481 agccaagcca caaggagtca aactagtgat caaatcaatt gatgactcga ttgccgcgtt
     3541 gaaaagcggg gtgacagata aatataagtt attcatcgta gtggaatcga tcgaggatgc
     3601 atataagctg gcagaagcgt atccagaaat caaaaccatc aacttaggag ggatcaaggc
     3661 aaaacctggt actcgcagca tcggaaaagc ggtaaatatt ttaccagaag aggaggaacg
     3721 cttgaaacga ttaattgaca aacaaataga agtagaaatc cgtcaagtac cagcagataa
     3781 aaaagtggat gtaagagaag ttttgtaaga aaggaagaac ctcaatgatt gtacaagcag
     3841 tcttattagg aattgttgca tttgtcgcac aatcagaata tgcactaggg acatcgttgc
     3901 tttctcgtcc gattgtgacg gggctgttta caggaatcgt gttaggtgat gtaaaaactg
     3961 ggatcattat gggcgcaact ttagagttag catttatcgg atcgttctca gtaggtgctt
     4021 cgattccacc tgatgttgtg accggtggga ttttagggac ggcttttgct attacagctg
     4081 gagccggtac agaaaccgcg cttttactag gtttgccgat tgctacgttg acattgatat
     4141 tgaaaaatat ttacttaggg cttttgattc caattatgaa ccataaagca gacaattatg
     4201 cagaagaagg aaattataaa ggagtcgagc atatgcactt gcttgccggt atcggtcttt
     4261 ccttgatgct tggtgttgtg gtaatggttt cttatatggt gggaagcaat acgatcggaa
     4321 atctattaaa tatgattcct gacttcgttc aaaaggggtt gtcagtagct acagggttga
     4381 ttccagctct tggttttgcg atgcttgctc gtttgctgat caacaaacaa gttgcaccat
     4441 attttttctt aggttttgct atagcttctt acttagagat tcctgtgacc ggagtagcta
     4501 tttttggtgc gattttagca gttgtggtag tcaatattat gaatgtccgt caactaccac
     4561 ttcaaacaac tgcaggggag gtagaagaag atgaagaatt ctgagatcaa aaacagcaaa
     4621 ataacgaaaa aagagctaaa tcacgtattt tggcgttctt ttcaaatgga gttttcttgg
     4681 aattatgaac gtcagatgaa tatggcttat gtttatgcaa tgattccgat tttgaaaaag
     4741 ttatatcagt cgaaagaaga aatggcagca gctttgaaac ggcatctaga gttttttaac
     4801 acgaccccac atatcgttac actgatttta ggaatcacgg ctgcaatgga ggaagaaaat
     4861 aaggatgatc ctgaatttga tgtgactgcg atcgatagta tcaaaacatc tttgatggga
     4921 cctcttgcag ggttaggaga ttctttcttt tggggaactt tgcgattgat tgccacaggt
     4981 gtgggtacct ctttagccat gcaaggaaat attttaggcc ctatcttgtt cattttgatt
     5041 ttcaatattc cgcacattct gtttcgctat ctagcaacag gttggggata tcgcttagga
     5101 acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg aaagtttgac attaggcgcg
     5161 tctattattg gactgatggt cgttggagca atgactgcca cgatgatcac catcaatatt
     5221 cctttaaaaa tcggttcagg agaaaatgct gtgactgttc aaagtatttt tgatgatatc
     5281 gttccaaata tcttatgtct tggagcattt ggcatagtgt tttatctatt gaagaaagaa
     5341 gtgaaaccat taacgatctt gatcggacta gctttctttg gaatttttgg ttcgtggata
     5401 gggatttttt aaaaaaggag cagagaaaat gtttacgatg caagattata tctatgaaga
     5461 aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt tcgacaagag aacatatgaa
     5521 aaaaacagtg aatttactga tcttagcaac gggttcatct tataacgctt gtttagcagc
     5581 gaagccagca ttggaatctt atggtgatct tactgtcgat attcaagaac ctttttattt
     5641 tttcaattat ggaaaacttt ctccttcaat cgatactgtg attgctgtgt cacaaagcgg
     5701 gaaaagtgct tctactgttg aagcagtaaa aatgatacaa aaacaagggt tacctgttgt
     5761 tgctatcaca aatgacgtgc aaagtccact tgctttagaa gctgcacaaa ttattgattt
     5821 aggtgccggg gtagaatcag taggcttcgt tacaaaaggc tattctgcga ctgttttaca
     5881 gttactactc ttagggatag gaattggaat aagtaaaaat aaaatcagca aaaaaataga
     5941 acaagactac atgcaacagt tgcgcaaaat aatcaaccat ttaccagcga ttattcaaaa
     6001 aacggaagaa ttttttgatg gatatcaaag tttatttcgt ttagcacagc gtttcgtcac
     6061 cattggatat ggtccaaact ggggaacagc taaagaagca gagacgaaat tgactgaaac
     6121 aatccgcgtt ccgtcacaag gatttgagtt agaagcttat atgcacggcc catacctgga
     6181 ggcagatgct tcgcatttgc tattctttat tgaaggggaa agtgtaaata aggaacgttc
     6241 gcaaaagttg caaagatata tgagtcggta cgttggagag actctcacca ttaccacaaa
     6301 aaaagcaaga aacgagaaaa cacttggatt agcaatagaa tgtgatgaat atctgtctgt
     6361 attagcgctt gtcgttccgt ttcaactatt cgcttataaa accgcagtag ctaaaggtat
     6421 tgacttgaac aaaaagattt ttgaagactt tgatacagtc ttgaaaagta aattataaga
     6481 ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc aaagcaataa aaggcgcact
     6541 agctttacgt ccgcaagtag aagaaatcat cgataaactt tatgcggaaa aatttgacgc
     6601 tgtctattat ctaggaatcg gtggaacgta tgcttcctcg atgcaagctg tcacttatat
     6661 gaatgggaaa agcaacttgc ctgtctttgt acagcatgct gcagaatatt acactactgg
     6721 aaacaaaaga ttaacaaaag attctatcgt tattttgtcc tctgttaccg gtacaactca
     6781 agaagtagtg aaagcagtag aacaaatcaa gaaagtagga gcgacactga ttggatttat
     6841 cgataaagcg aacagtaagt tgtctcagct atgcgatttt gtggtcactt atccagctcc
     6901 tggaacagaa caaatcaaat tttttatggc agcagatcga ttgatgtatt tgaatggaga
     6961 attcgaagct tattcagaat actatgaaca gctcgaacgt tatttaccaa cagggttagt
     7021 ggaagcagag aaaaaagcag atgcatttgg cttatcattt gcagaaaaac atcgacgtga
     7081 tagtatgcat tattttattg gagcagggaa tcaatgggga gctgtttatt catatgccat
     7141 gtgttactgg gaagaacaaa gctggcttcc atcgaaatct atccatgctg ccgagtttct
     7201 tcacgggacg ttagagatcg tagaagaaac aaccccagtc acattatttt taggcgaaga
     7261 tgaacagaga gtgttatctg aacgtgtggc taaacttttg ccaaaaattt gcagcaatta
     7321 tacattgata gacacaaaag actatccagt agaaggtatc agtgaaaaat atcgcggacg
     7381 tgttttgtct ttcttattga tgcatgtagt gacacaacgc atcgatgctc atgtggagca
     7441 gttgaactgc catccattag agatacgccg ctactatcgt cagtttgatt attaggatag
     7501 actggataaa aagaatataa aaaataaaga gagcagcgat aactgctccc tttatttttt
     7561 taaaatattg gcatcaatta cgttataaaa cacatttttt gcattatcta cagttcaact
     7621 tataatctgt ttaatgggac atgtgggtgc caaccatttt ttgttatgca atagtactat
     7681 ttagttgttg catggatccg tagtaaaagt ccaagtacaa cgagttgcgc cagtttacat
     7741 agcgttttgt atccagaatg ttgactaaaa caaaagttcc tatgtcaacc gtcaacagag
     7801 acaatctgtc cgttgcagga cctgacagat ttagaaatgt tcaatctgtc tcttatgtat
     7861 tttaaatcaa tactcactag gagaagtttc ctaacctcct aatagtaatg ctggtagtaa
     7921 cattgtcaaa actgcttcct cttaggaaca agcaaagcat aacgagtgta aaaataaaat
     7981 gtagactatt gtaacatatc attgattatt tcaagtaaaa acgataaaag
//
LOCUS       DAZKNM010000123         7947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000123 DAZKNM010000000
VERSION     DAZKNM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.125"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1013
                     /locus_tag="ACG8M9_002140"
     CDS             150..1013
                     /locus_tag="ACG8M9_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0378865.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1142..2764)
                     /locus_tag="ACG8M9_002141"
     CDS             complement(1142..2764)
                     /locus_tag="ACG8M9_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0378866.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3013..3534
                     /locus_tag="ACG8M9_002142"
     CDS             3013..3534
                     /locus_tag="ACG8M9_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0378867.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3720..5450)
                     /gene="spxB"
                     /locus_tag="ACG8M9_002143"
     CDS             complement(3720..5450)
                     /gene="spxB"
                     /locus_tag="ACG8M9_002143"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0378868.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5615..6433
                     /locus_tag="ACG8M9_002144"
     CDS             5615..6433
                     /locus_tag="ACG8M9_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0378869.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6536..6733)
                     /locus_tag="ACG8M9_002145"
     CDS             complement(6536..6733)
                     /locus_tag="ACG8M9_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378870.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6902..7480
                     /locus_tag="ACG8M9_002146"
     CDS             6902..7480
                     /locus_tag="ACG8M9_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0378871.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7575..7712
                     /locus_tag="ACG8M9_002147"
     CDS             7575..7712
                     /locus_tag="ACG8M9_002147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378872.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2390 a         1410 c         1575 g         2572 t
ORIGIN      
        1 cttcttattt cttgaagctg accacttctg tcacaacctc ttattttgat tagaacttgc
       61 gaaaataaac cggttaaagt gtgtctagcg caagagattt gctataatag acgataaggg
      121 taaaatgtaa cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg
      181 atctgccata ccatgtatta gcaaaagaaa aagttgattt tttatcaatg aatctccaat
      241 tgaatgatca agagttagta gatgacctcg gaaagacatt caattatgac cgctacatga
      301 atcagctgaa aaatggtgcg atgcctacga catcacaaat caatgttggc cgatatttag
      361 aatttttccg ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct ttttccagtg
      421 gattaagcgg ctcttacagt agtgcgcttc aagcggttga attgctgaag gaagaatatg
      481 aagaccctga gatctatgtt tttgatacaa aagctgcaag tattggggaa gggcttttag
      541 tattggaagc tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc
      601 tggaaagtaa ttatttgaaa gtccattcat gggtaacagt agacgatctg aaatatctag
      661 aacgaggcgg taggatttcc aaagcaagtg ctactcttgg cagtctattg agtgtaaagc
      721 ctattattac ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa
      781 acaagtcgat ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc
      841 aaatcgttta tgtggcttac gctggggatc tagagagcgc ggagaaggca aaacggcttt
      901 tggaagagca gataaagatg aaagatgtga agctttatcc cttaggacca acgatcgcca
      961 gtcacacagg ctatggctgt atcgcgattt tttcgatggg aatcagtcga taaaagcagt
     1021 aactcattct gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac tcttaaaaga
     1081 tagatgagca tcctatctct tagaggtcgc tgttttttta ttttttaagc taaataggcc
     1141 attatgccca taactcgttc aattgttttt gaatatctgc attttccaaa aattcatcat
     1201 atgtcgtatc agctcgatcg atcacacctt tatccgaaac ggcaatgatg cggtttgcca
     1261 atgtttggat aaattgatgg tcatgtgacg caaataagat tgagccagta aatgccatca
     1321 agccgtcatt caatgctgtg attgattcta agtctaaatg gttcgttgga tcatctaata
     1381 caagtacgtt agctttcgaa agcatcattt ttgaaagcat gcaacggact ttctctccac
     1441 cagataaaac attcactggt ttcaatactt cttcaccaga aaacaacatt cttcctaaga
     1501 agcttcttaa gaaggtgtta tcatcttctt ctttacttgc atattgacgc aaccagtcta
     1561 aaatcgttag atcagtatcg aattctttcg ttgtatcctt tggtaaataa gaattacttg
     1621 ttgtcacacc ccaacgtact gttccagtat ctggcgtaat ttcacccatg ataactttga
     1681 atagcgttgt tgttgttata tcattttctg cgacgaaagc gaccttgtct tctttgctca
     1741 aattaaaaga tatatcatct aaaattttct ttccatcaat cgttacactt acattttcta
     1801 cctgcaataa gtcgtttcca atctcacgtt caggtttgaa tccaacgaat ggataacgtc
     1861 tagaagaagg ttggatatcg tctaaagtaa ttttttcaag cattttttta cgtgaagttg
     1921 cttgttttga ttttgatgca ttcgcactaa agcgggcaat aaattcttgg agttctttga
     1981 tttgttcttc ttttttggca tttgcgtttg cttgaagttt agccgctagt tgactagatt
     2041 ccaaccaaaa atcataattt cctacataaa gtttgatttt accaaaatct agatccgcca
     2101 tatgtgtaca tactttgttc aaaaagtgac ggtcatggga tacaacgatc acagtatttt
     2161 caaaattgat caaaaattct tccagccaat tgatcgattg gatatccaga ccgtttgttg
     2221 gttcgtctag caaaagaaca tcaggtttac caaatagtgt ttgtgctaat aacactttta
     2281 ccttttgtcc accagttaat tcactcattt gctgatggtg caattcttca gggatgttca
     2341 gaccttgcag taaaacagct gcttctggtt cagcttccca tccattaagc tcagcaaatt
     2401 ctccttctaa ttcagctgct cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa
     2461 tcgcatcttt ttctttcagc acttcataca gacgcttgtg tcccatgatg actgtttcaa
     2521 gtacagtata gtcttcatag tcgtaatggt tctgtttcaa tacggccatc cgttcatctg
     2581 gtcccatagt aacggaacct gtggaaggct gcagatcccc tgataacact ttaaggaacg
     2641 ttgatttacc tgcaccattt gcaccaatca agccatagca gtttccgggg gtaaatttaa
     2701 tattcacatc atcaaatagt ttgcggtctg aaaagtgcaa actcacatca tttacagtaa
     2761 tcaatttttt tcctcacttt tatcattatt agttttgaca agtcttatag aattatatag
     2821 gggaaaggta aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg
     2881 aaaaaaataa aaataaaagc ggagcgaaaa tagctactat aggatttatt agtttgcaac
     2941 agcataattt ttgacgttat ttataagagg gagtactata aaactgtaaa agaaatgaaa
     3001 gaaggattga aaatggatga aaaaagaacc atcgactggg gctcattgat tttgggaatt
     3061 ttatttgtac ttgtctcatt gttatcattt caagaccctg tcggaaattt agttgcaatt
     3121 gtagtagtct ttgcgatttt tgcctttatt aaagggatat ttgaactgtt tgtacggaac
     3181 agattaaaag agctaacagg atataaagga aagatgccct taataatcgg gatcatcgat
     3241 attctagttg gcgtcttttt cttattcaat attggtgcag gagtcgcagc attgcctttt
     3301 gtttttgcag tatggtttat tgctgattca gttttagcgt tatttactgc tgacttggca
     3361 agaggagtaa gtgaaggata ttattggttt acgatcatcg tcaatatttt aggaatttta
     3421 ttaggaattt tcttattgtt taatccaatt tcttctgcgt tgacattaag ctttttagtt
     3481 ggattctatt ttatgttatt tggtatcaca catatcgtct atgcatttag ataagtaaaa
     3541 tcaaacaaat aaaaaagccg gcttccgatt acagttttac tgttgtaatt ggaagccggc
     3601 ttttttatca tggacaaata tgcacgaggt gatttttagg tgtcgagaca aatttatgca
     3661 aaaatgtgag aagctatata cacaaatttt atcttaatac tcgaagcata gcgcagttgt
     3721 cacaacctct tttcatataa ttcattcaat actggcaaat catggacttc atatttttct
     3781 ttgaatgctt ggatttcttc ttcagaatat tttcttggat ctaaatgcag atcttctact
     3841 ggcaatgggc gcttgttatt gatcttcaca tcgattacta caggacgatc tgattttgca
     3901 gctgcatcaa aggcaaatcg aagttgcgag tattctgtga ctgtaaagct atttgccccg
     3961 agcgcttctc caactttacc gaaatctgca tcttctaaaa atacaccaaa tttcttttgt
     4021 tccgtatctt cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta
     4081 ataattggaa gctgatattt gacttgtgta atgatatctt gcatgaccat agcaaacccg
     4141 ccatcaccac ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc ggcaattcct
     4201 cctggcacac cattacccat agttgcgaac catccagatg tcgtatgttt ttgtttaccg
     4261 ttcatattca ataaacgaat agaatggata gtcgtgttac cgacatctgt gacgaaaatc
     4321 gcatcgtctt cagcaatacg attgatttct ttatagacag cctctggacg aatcggttct
     4381 tcctctcgat cttcaaaaga tttcaaccaa tttacccatt tttctttatt ttcttgattt
     4441 gctaggtacc agctatcagc agggcgagca tcccctaact cgacaagtct ttgcaaagct
     4501 gtgtttgcat caccgaggat cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca
     4561 tcgatatcga tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag
     4621 tcgctaccga caaataagat cagatcggct tctgccaatg cttcatttcc gggtttagta
     4681 gcgacacgtc cagcaaaacc aagaaagttt tcataatcat caggaataat tccttttgct
     4741 agaacggaag cgacaacagg aactgaaaaa tgttctgaga attctttgat ttgcggaaat
     4801 ccgtttcgcg ttccttgacc aacataaaga actggttttt ttgctttttc aagataaggc
     4861 aaagcagcta gcaaatcttt ttcttctggt aatatcacac ttgttttgtg agtgtgagcg
     4921 gtggagaaag gttgttcgtc aatttcttca aaaccaagat caacagggat agtcacgata
     4981 gccacgcctt tatgctcata ggcggcttta atggcttcat ctactacatg cggaagactt
     5041 tgtggtgtca tgactgtacg attgtatacg ctaacatctg cgaaaatagg attttcattt
     5101 agttcctgga aagagttata gttcatggat gtagaagcaa cttgtcctaa gaacgctact
     5161 agaggcacat ggtccatctg tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt
     5221 cctgcagagc cgaagacagc gccgactttc cccgttaatt ttgcatctgc agcggctgcg
     5281 agtgcgccga cttcttcatg acgaacttga atgtagttga tttcggtttt ttcttgatat
     5341 agcgcatcca taattgaatt gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc
     5401 cagctttcaa gaacttttac cattgcgatt cctgcattga tttttgacat ggtgtttcac
     5461 tcctttagca atgatgatac ataaattttt tgtatctaga gtatagaact ttttatcgaa
     5521 aatcccaact aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag
     5581 tagtacatag atagattcaa gagaggggaa tggcatggtt gaatggctat tgagacttga
     5641 accttggcag caagcacttg ctgggacagc ttttacttat tttatgacag ctcttggtgc
     5701 cgggttggtt tttttcttta aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt
     5761 tgcttctggt gtcatgattg ctgcaagttt ttggtctttg ttagatccgg ctattacaaa
     5821 agcagaagaa aatggagata ttgcttggct agttgtaagt attgggtttg gtttaggcgg
     5881 tgtgttttta tatatggcag ataaaacact gccccatatg cattttggac cacaacatga
     5941 agcagaagga ttgcctaccc atttaaaacg gacgatcttg cttgtttttt ctattacatt
     6001 gcataacatt cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag caactgcgga
     6061 taatccgaca gccgctatat tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa
     6121 cttcccagaa ggagcagctg tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa
     6181 ggcctttgtt tacggacaag cttctggaat cgttgaacca attgctggag tgattggagc
     6241 gctactagta acgaaagtgg agctgctgct tccttatgca cttgcttttg ccgcaggagc
     6301 aatgatttat gtcgtggtgg aagaattgat ccctgaagca cagcagacat tgtccagcaa
     6361 acgtcatttt gcagtttttg gcgtcatgag tggatttatc attatgatga ttttggatgt
     6421 ggcattagga taaataaaaa aaatatatat gatatatcaa atagggccgc gaacttatat
     6481 cgcgacccta tttttttagt aagttatatc ataatgaagt gaatggtatc atacattaat
     6541 aatagttttt tctatgtgta tttcctttga tccagcgaac aagccagatg ataagtgcga
     6601 ttgggattag aactttgacc gcaaaccata aaagcccgct taggacactg acaaaaagac
     6661 tgatgacgaa gctagctgca attagcacta aaagaaccat gaagatattt aagaacatat
     6721 ttttttgacg catacaaaac cctcctcttg ttcattcttt actatagaaa aagtataaca
     6781 gggattgttt aaaaacacct ccaactgaag actgattttt acttcatcta cttaaatgat
     6841 caaacaattg ctttttctct ggtttatcat ctaaaataga gcggaaaagg aggagaatat
     6901 catgcctaaa aatcgtcttg aagcatttac agatgctgta atcgcgatta tcatgactat
     6961 tttagtttta gaacttcatg aacctaacgg agacacgtgg gaggcgctcg ctgcattagg
     7021 ttaccgattt tttatctata ttgtcagttt tatcatttta gcgatttatt ggaacaatca
     7081 tcatcattta tttcaagttg tacataagat aaatggacgg gtcttatggg cgaacagttt
     7141 ctttgttttt gcgctttcgc tttttccgtt tgcaacttca tggatcagtg agtacatcaa
     7201 tagcctggct ccagaaatga cttatggttt tgttaccttg accgctaata ttgcgtatta
     7261 tatcttggca aaagaacttg tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat
     7321 gttgaataat tacaagaaat cgtatctatc gattggtttg aatgtgttag gaattgtctt
     7381 agggtattta gtagcgccag tagccgtgct gatcgcaaac atcgtgattc tagctgcttg
     7441 gatcatccca agtcgtacca tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt
     7501 taagagaaaa atatagaaaa aataaataaa cgatcaaata tccgagtgat actagtattc
     7561 aaagagggat gcagatggat caaaaaagaa ttcatgtagg actgattact agttttgtca
     7621 cgatgcttat ttatttggat caaggtcatt tgacttggac agtagttggt gtcgtaggat
     7681 ttttgacatt attcacagca ataaaaaaat aaacatcttt atgtaatctg atgttctatc
     7741 tatttaagaa aagcatgttt acagcagtat gattctgttg taaacgtgtt tttatttgtg
     7801 gaaaatttaa tttttagaaa agtgtatcga aaaatagata gttagttttt ccacagtgga
     7861 taacttcgta agagataaaa ataaaaaatt agtcaaagaa gggcgtatag atttcttttg
     7921 ataggagata tacaacagaa atgaata
//
LOCUS       DAZKNM010000124         7865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000124 DAZKNM010000000
VERSION     DAZKNM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.126"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..843
                     /locus_tag="ACG8M9_002148"
     CDS             142..843
                     /locus_tag="ACG8M9_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0378873.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1452..2468)
                     /gene="tsaD"
                     /locus_tag="ACG8M9_002149"
     CDS             complement(1452..2468)
                     /gene="tsaD"
                     /locus_tag="ACG8M9_002149"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0378874.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2465..2929)
                     /gene="rimI"
                     /locus_tag="ACG8M9_002150"
     CDS             complement(2465..2929)
                     /gene="rimI"
                     /locus_tag="ACG8M9_002150"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0378875.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2936..3478)
                     /gene="rimI"
                     /locus_tag="ACG8M9_002151"
     CDS             complement(2936..3478)
                     /gene="rimI"
                     /locus_tag="ACG8M9_002151"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0378876.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3462..4184)
                     /gene="tsaB"
                     /locus_tag="ACG8M9_002152"
     CDS             complement(3462..4184)
                     /gene="tsaB"
                     /locus_tag="ACG8M9_002152"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0378877.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4375..5355)
                     /locus_tag="ACG8M9_002153"
     CDS             complement(4375..5355)
                     /locus_tag="ACG8M9_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0378878.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5379..6863)
                     /gene="galT"
                     /locus_tag="ACG8M9_002154"
     CDS             complement(5379..6863)
                     /gene="galT"
                     /locus_tag="ACG8M9_002154"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0378879.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6875..7864)
                     /gene="galE"
                     /locus_tag="ACG8M9_002155"
     CDS             complement(6875..7864)
                     /gene="galE"
                     /locus_tag="ACG8M9_002155"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0378880.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2202 a         1695 c         1392 g         2576 t
ORIGIN      
        1 ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa acttgtttct
       61 aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa
      121 caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc
      181 agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat
      241 actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa
      301 cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact
      361 tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga
      421 gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct
      481 gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa
      541 aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc
      601 gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca
      661 aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc
      721 ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc cgggctgtta
      781 gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa
      841 taacgcgaac aaaaataagc agtgaccctg ataaatatca gggtcactgc ttattttcct
      901 ccctaaaact tctgaaagat ccggcagttt tagatcactg gacaagactc aaagattttc
      961 tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgtta gattaactag
     1021 atgaagcgtc ttgtcctcat ccgatctaga ttattcgata ttcctcgaca ataaacgatt
     1081 actcagtgag ggcaatcaga ggaacggata atgataaata agctgtaaaa aatagcaaaa
     1141 taacaataat gattaaaagt atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc
     1201 tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata atgttcataa
     1261 acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg acaggcttac
     1321 gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc
     1381 aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag ggcagctttt
     1441 tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg atagctagca
     1501 aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc attatctcca
     1561 caaagtcgaa gagggggaat cactagttct acctcaggga gtttagttgc caaagcatcc
     1621 tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctataat cagttgcttg
     1681 actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat cacacttgct
     1741 tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg tgcattatgc
     1801 accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt atcttcatgg
     1861 atcatcgctc gcggaaaatg gtaattatct tttccctgat gagctagctg atcgatttct
     1921 tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc ttctcctgct
     1981 gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat atagactaat
     2041 tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg tttaaccaga
     2101 cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc
     2161 caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag tcctggtcca
     2221 taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac cagtgcatct
     2281 tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg tactacgcca
     2341 ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat ctctgttcca
     2401 tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag aatcaattct
     2461 tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca tcttccgtcg
     2521 gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat tcataaagtt
     2581 ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa tcttttattt
     2641 gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg
     2701 caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca ataaagccac
     2761 agatctggtt atcttcagct aaaagcagat aatcagagtg aggctgttgg atgtctgcca
     2821 aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta atcatccata
     2881 cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac taatctcact
     2941 cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg taatatcttt
     3001 ttttgatcgt tgttgcttga aaccccaatt tccgataaag tctttgtgca tcttgattac
     3061 tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag ttctcgattt
     3121 cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg gcaatattcg
     3181 tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt cttccattat
     3241 caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata aaggcacttt
     3301 ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc tctttgatat
     3361 cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgact gttttttctg
     3421 tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt ttcaacataa
     3481 ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac tcgttttaaa
     3541 tagcgaggaa gaaaagcttg tttctctgca actggttcaa ctaaagcacc taattgagcc
     3601 acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc actaggcaat
     3661 atttccttta tttcagagaa gaatttgtga caatcactcc cgacgaaata aatagcttca
     3721 ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca
     3781 gtttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt tcttcgagca
     3841 tcaaacatgg gcacaataat attatccaca ccgatgcaat tagccgcgat ggttttcaga
     3901 ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc tgtcgttaca
     3961 cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct
     4021 tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag tgttacactg
     4081 tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc taccacaccg
     4141 atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt gatcctttct
     4201 gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat gaaaatacca
     4261 aaggcaaaac tagtacaata atacatgatt atctgctggc gggcaaattt tgccccttcc
     4321 tgaaacacaa aaaagctgcg gtatcactcc attttcccac agcttttcca tcttttattt
     4381 cgttgattct cgaataatca actctgtttc cacaataatc tttcttgaaa ccggtgcttt
     4441 ggaattactt aattgaagaa tcgtctccac tgctagctct cccatccatt cggtataaac
     4501 ttttacagta gaaagcgagg gcgttacata tttcgctaca ctgacatcat tgaagcccat
     4561 cacagaaata tcttctggta ccactaaacc tttttcttga atcgctttca gtgcgccaat
     4621 cgctaatgca tcattagctg caaaaagcgc atcaggaaca tgcggattcg tttttaaata
     4681 ctgcttcact gcttgatatc ctgactcgac agaaaaatct gcttcgataa ttggaccttc
     4741 gtcgtagcct ttttctgcca aaacttgttg aaatgccata attctgggat cttttagccg
     4801 ctgatggttt ttcttcgtat attctgttcc agcaagcata gcgatatgtt gcgctttatg
     4861 ggagacaaag tgttcaatga ctttttgaac acttgactta aagtcgacaa ctagagagtc
     4921 gattccttga gaagtgccgt cagaatctac caaaagcaag gcattttggg atcctttcat
     4981 ttctaataac tgagcttcgt cgaattttcc tagtgcgatc gttccatctg ccggaagatc
     5041 actaatggaa tcccacgttt ctttcaataa agtaacgttt gcttcttctg cctttttttc
     5101 gattcctagg cgaatagata aataataaag atcttcaagc tcttccgtat cgtcgtacca
     5161 ctggatcagg cgaagcgttt gcttagtatt tgctgcattt ttacgatgtt tcgtataatt
     5221 taagcgttct gctgcttcaa aaattttttg ctttgtggct aagccaacgg aaagatcagg
     5281 atcatagttg agaactcttg aaacagtagc aggagaaact cctgccaact gcgcaatgtc
     5341 tttaattgtt gccatttgtc tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt
     5401 tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt
     5461 gcaaaaacct gacctacaga tttttgtacg acatcggtga ccgtatcttt cgtcaaatca
     5521 ggatgttctt tttttaattg ttctgcccac ggacgatgat attcagccat ctggttttct
     5581 tctcctaata agaaatgctc tacttctact agttctggtt tcaaacgagg tggtaaaaca
     5641 gcaagcccca ttacttcgat caacccaatg ttctcctttt tgatatgttg gacatcttta
     5701 tgtggatgaa aaatcccatc aggatgttct tctgaaacat tgttgtctcg aaggacaagg
     5761 tccaactcaa agtaattgtc ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta
     5821 ccatctgctg aaaaagctct gatagatact gtttcatccg aatatgcttt ccatttttcc
     5881 aatactgagt tagctgcttc aataagcaag tttttctctg ttgattgcaa acggatcaca
     5941 gacatcggcc atttcacgac tcctgcgatg acttgcggat aatcttctag tttgataagt
     6001 ttttctatcg gtgcttttgc cataggaaat tcatgacgtc ctgcctgata atgatcatga
     6061 gaaagaatcg atcctccaac aattggtaag tcagcattcg aaccaacaaa gtaatgaggc
     6121 aagacttcag taatccgaag cagtctagca aatgtttcct ttgtgatttt catcgggcgg
     6181 tgttgttctg ataaaatgat cgaatgttca ttgtaataag cataagggga atattggaat
     6241 ccccagcttt caccgtctag attcattcga ataatccggt gattggtgcg ggctggatag
     6301 ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca tgcaggtagg atagtctact
     6361 tttaccgcct cgcgttctgc tgcgatttgt tttggatctt tttctggctt agataaattg
     6421 attgtgattt ccagttcacc atactccgac tctgccggaa atttgatgtt ttttgcgatg
     6481 gctcttgttt tgatgtagtc atttttctta cttaaattaa agaaatactc agttgcttcc
     6541 tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg
     6601 agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca
     6661 ccattttctc ttgccttagc gacaagctga tccaataagt ctacagattc ttccttaact
     6721 ggtcttactt caatgctttc tagagcatct tctcctacca tccctaaaag acgattctgt
     6781 aaatataagc ggtccatttc catccatccg ccagcttcga tcgcaagtgt ggcaaaatca
     6841 caaatcgttt gactgattga catatctcat tcccctattc ttcataacca tttggatggc
     6901 tgacatgcca atcccatgct gttttgataa tcgctttgat atctgtgtat tcaggtttcc
     6961 agcccagaat ctctctcgct ttttcacttg aagcgactaa tcggctagga tctccagctc
     7021 ttcttggtgc tatttctgct ggaatttctt ttcctgtcac ttctctagct gcttctagca
     7081 tttccttgac tgagtatcct ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt
     7141 ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa atcttctact tgaacataat
     7201 ctctgataca tgttccatct ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc
     7261 tttgaccaag agctacttgt aaaatgattg ggacaagatg cgtttctggt gtatgatctt
     7321 ctccgataga tgcatctgct tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc
     7381 gcatgccata tgcttggtca caccacttca tcattttttc catcatcaat ttactttcac
     7441 catacggatt tttaggatta gctggtgtat cttctgtgat tggagactct ttcggctctc
     7501 cataagttgc tgcagtagaa gagaatacga ttttattcac gttgaactcg tgcatgactt
     7561 caagtaagat ttgcataccg tatacgttgt tgttgaaata catcaatggt ttttcgacag
     7621 attctcctac taaagagctt gcagcaaaat ggattactcc ttcaatcggt tccttttcaa
     7681 atacggaacg tagaaattct ttatcccgga tgtctccttc ataaaaatga gcatctggat
     7741 gtactgcttg tttgtgacct gtgagcaaat tatctaccac agctacttga tacccctttt
     7801 gaactagctg gtctacagcg tgcgaaccga tataaccggc tccgcctagt actaaaattg
     7861 tcata
//
LOCUS       DAZKNM010000125         7795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000125 DAZKNM010000000
VERSION     DAZKNM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.127"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..631)
                     /locus_tag="ACG8M9_002156"
     CDS             complement(<1..631)
                     /locus_tag="ACG8M9_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0378881.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSL"
     gene            complement(633..1397)
                     /locus_tag="ACG8M9_002157"
     CDS             complement(633..1397)
                     /locus_tag="ACG8M9_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0378882.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1405..2082)
                     /locus_tag="ACG8M9_002158"
     CDS             complement(1405..2082)
                     /locus_tag="ACG8M9_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0378883.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2119..3504)
                     /locus_tag="ACG8M9_002159"
     CDS             complement(2119..3504)
                     /locus_tag="ACG8M9_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0378884.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3714..3798)
                     /gene="ffs"
                     /locus_tag="ACG8M9_002160"
     ncRNA           complement(3714..3798)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8M9_002160"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3821..4336)
                     /gene="tadA"
                     /locus_tag="ACG8M9_002161"
     CDS             complement(3821..4336)
                     /gene="tadA"
                     /locus_tag="ACG8M9_002161"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0378885.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4443..5300
                     /locus_tag="ACG8M9_002162"
     CDS             4443..5300
                     /locus_tag="ACG8M9_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378886.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5381..5716)
                     /locus_tag="ACG8M9_002163"
     CDS             complement(5381..5716)
                     /locus_tag="ACG8M9_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378887.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5783..7153)
                     /locus_tag="ACG8M9_002164"
     CDS             complement(5783..7153)
                     /locus_tag="ACG8M9_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0378888.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7231..7767)
                     /locus_tag="ACG8M9_002165"
     CDS             complement(7231..7767)
                     /locus_tag="ACG8M9_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0378889.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2215 a         1665 c         1266 g         2649 t
ORIGIN      
        1 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
       61 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
      121 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
      181 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
      241 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
      301 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
      361 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
      421 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
      481 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
      541 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
      601 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
      661 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
      721 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
      781 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
      841 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
      901 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
      961 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     1021 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     1081 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     1141 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     1201 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     1261 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     1321 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     1381 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     1441 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     1501 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     1561 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     1621 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     1681 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     1741 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     1801 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     1861 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     1921 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     1981 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     2041 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     2101 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     2161 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     2221 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     2281 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     2341 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     2401 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     2461 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     2521 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     2581 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     2641 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     2701 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     2761 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     2821 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     2881 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     2941 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     3001 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     3061 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     3121 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     3181 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     3241 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     3301 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     3361 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     3421 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     3481 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     3541 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     3601 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     3661 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     3721 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     3781 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     3841 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     3901 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     3961 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     4021 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     4081 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     4141 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     4201 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     4261 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     4321 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     4381 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     4441 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     4501 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     4561 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     4621 aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     4681 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     4741 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     4801 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
     4861 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
     4921 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
     4981 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
     5041 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
     5101 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
     5161 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
     5221 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
     5281 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
     5341 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
     5401 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
     5461 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
     5521 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
     5581 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
     5641 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
     5701 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
     5761 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
     5821 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
     5881 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
     5941 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
     6001 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
     6061 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
     6121 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
     6181 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
     6241 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
     6301 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
     6361 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
     6421 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
     6481 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
     6541 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
     6601 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
     6661 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
     6721 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
     6781 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
     6841 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
     6901 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
     6961 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
     7021 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
     7081 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
     7141 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
     7201 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
     7261 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
     7321 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
     7381 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
     7441 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
     7501 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
     7561 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
     7621 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
     7681 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
     7741 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc taccc
//
LOCUS       DAZKNM010000126         7725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000126 DAZKNM010000000
VERSION     DAZKNM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.128"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..763
                     /locus_tag="ACG8M9_002166"
     CDS             83..763
                     /locus_tag="ACG8M9_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0378890.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            853..1887
                     /locus_tag="ACG8M9_002167"
     CDS             853..1887
                     /locus_tag="ACG8M9_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0378891.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1901..2236
                     /locus_tag="ACG8M9_002168"
     CDS             1901..2236
                     /locus_tag="ACG8M9_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378892.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2220..4700
                     /locus_tag="ACG8M9_002169"
     CDS             2220..4700
                     /locus_tag="ACG8M9_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378893.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4756..6315
                     /locus_tag="ACG8M9_002170"
     CDS             4756..6315
                     /locus_tag="ACG8M9_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378894.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6312..7676
                     /locus_tag="ACG8M9_002171"
     CDS             6312..7676
                     /locus_tag="ACG8M9_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0378895.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
BASE COUNT         2557 a         1413 c         1551 g         2204 t
ORIGIN      
        1 aagtaagcgg atgaattcat gaggattttt tgcatactgt ctctactata cttaggtcac
       61 taagaaaaag gagtgattta ttatgaaaaa gaacatgatt gggacattat tactttcaac
      121 acttcttcta ggcggcttgg caacaccagc ttttgctgag ggtcaagcga cttcaaaagg
      181 agatattacc tttactgaac caacaaatac cgtggagcct cttaacccta ctgatccttc
      241 aaaaccagta gagccagcag atccggaaaa tccagcaact ggccagacag gtcctttaac
      301 actggatgta gttcccgaat tgccatttgg tacacacgag attgaaagcg gaacaaaaac
      361 gtatcaagta gacgcttcaa aaaacgatac accttatctc caagtatcag atcgtcgagg
      421 tgtaggtgca gatggccaag cacaagggtg gaacgtgaca gtttcggttt cagattttgt
      481 aaacggttca caagttttgc aaggcgctga attagatttt ggaacatcca ctgttaaaag
      541 tacgtcagat aacgaatcca ctggaccaac tagtcaaact gtaactgggt tgagtaaagc
      601 aagtgcagcg acaccaatat ttacagcagc aaaagatcaa ggtctgggaa catggctttc
      661 tgtttatgat ccagcaaata tcacactgaa agtgccgaaa gcagcagctg gaacattcac
      721 ggctgatttg acttggaacc tcgtggctgg cccagtagca tagagtatga aataaattca
      781 acaaactgcg gtgaaaatac tataggtgaa ggatttttta ccgcagttgt tgttgaaagg
      841 atgtggtgga caatgaatca aagtaaacga ctgtttgttt cgtttttttc tattttaagt
      901 ctatttttca tttttccgtc aatcagccaa gcaaaggatt cagcaggaga ctttggaatc
      961 aaaccggtat ttccagagaa ccaaatagat aaagcaatcg gttattttga tttattagtg
     1021 gcaccggaac aaaatcagac actagaagta attatcagca attcttcaga tgaagaacga
     1081 acatttgagg tctctgtcaa tccggcggta acgagtgatg gtggaacgat cgattatagt
     1141 caaaaaaacc caacgttaga tgagacattg ccgtttgatg tccgtgacgt tttgctcatt
     1201 gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa ctgtacctat agaagtgaaa
     1261 atcccagcaa aaagctttaa gggacgtgta ttagcgggta ttcatgtttc acctaaagaa
     1321 gaagctgaaa cagaaaatgc tacagaagga gcacaaatca aaaatcgtat tgcttataat
     1381 ctcgcagtcg ttttacaaga aagtcaagag acgatagaac cggatctgaa acttttatca
     1441 ggagatttgg acgaggtcaa cgcaaagcca actgttcagc ttcgtttcca aaatcctcag
     1501 cctaggatta tcagcaattt gatatttacg agcaagatct tttatgaaaa tcagctttat
     1561 attgaaaata cgtctaatgc atttttagtt gcaccaaatt cgaattttca cttgaatctt
     1621 gatttagctg gtgataaagc aaaagccggc gattaccgcg cagaaatcat agcaaaatca
     1681 ggcgacagta atgaatggcg atttacacaa aactttacga tcaaaaaaga aaaagcacaa
     1741 aaagtaaatg aaaattccgt ttttgcagta caagaacaat ctttcccatg gctttatgca
     1801 ggattggttg ctgcagttct agtgttatgg ttgattttgc tgtttttgtg ggtccacaag
     1861 aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg ttgaaaacat cgatcttatt
     1921 gggagggatt attgcaagtt tgctgttctt cagctggcaa gagccagtcc aagcagatac
     1981 caaaagttca ggtacgatta cattttatga tctaagtcca attccgcatc caagtggttc
     2041 agtcaatggc gcatcaaaac agcagacaca gctaccgatt tataaagaat ctaatcaaca
     2101 tggcgtattc ccggagacga atgaacaaaa gatccgtttt ctttatttga tcattggtat
     2161 agaattatgg cttcttgcgt ggttgttgtt atggagaaga aaggaaaagg aggatgaaca
     2221 tgttgctaca aaataaattt tcattttttc gctccttctt ttttacttcg ctttttgttt
     2281 taagtatatt tatcttttca ccttcggcag aagctgttgg agcaacgcct cctgccattt
     2341 cttatgatcc atacacgtat gaaaatatcg tacctccaaa tggtggacgt agtgcagtca
     2401 atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt gcctaaattg actgctactt
     2461 atttgaacga tggttcaaat gatccttgga aaggtggcac ggtccgtttt cattgggaaa
     2521 gccatgtgaa actaacaaat gtacttgatt tggtgaatat cactgcacaa ggatttgcat
     2581 ttgtggcgat catcaatttg ccggaacatg tatcaagcag tgatgtatta aaagcaattg
     2641 actggaacaa cgcctacttg tatattgccg gtgcccccat caaggttact agcggaagtt
     2701 tattatcagc aggaagccat accttgcgtt ttgggatggg gtcgtgggat acaaatactc
     2761 tattgacaga tattattgct attattacgc gaggcgaatt caacgtggac gatatcccgc
     2821 ttcaatttga tgttacagct aatgtcgcga agatgacaga aaacggagat aaatatgata
     2881 cgagtccaaa caaaattttg accaagggta aattacaacc ttctatgtct aaaaaggccg
     2941 ttttttctgt tgatttttat gattcgaata atattatcca agatcgtatg ttcaattcga
     3001 ttttagctcc agctgcaggt agattttatc ctaaattaac tggcggaaac ttgaatccag
     3061 gagcatatgc attttctgca agtgcagaga taaatacgtg gaatagttac atctcaccaa
     3121 gtgattcaac aggaaaatat aatacatttg ctgataccac agaatacgtg gatggaaata
     3181 accagaacct tccaggctct accaatagtc ggacattgac gatggggctg actgaaggac
     3241 gttttgatca gttgccattt agtcgctttg atcgcgtagt caactatttt acaggaaaaa
     3301 acgttacaca aggagcaata ctgactcata ctccagcaag cacaagtgga ttgaatcaga
     3361 cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg attgctttct cctgttgctt
     3421 taaaggtatt ggagcagtac aagctagacg gaacggtttt accaacaaac ttaacagccg
     3481 atagtcagtg gggaaagctc ctttcttctg catcgtacca agtcacggcc aattggtcag
     3541 caactgaact aaaaaagggt tcgatccatt acaggcttta ttcgaaagga acccaaaaat
     3601 tagcgacagg atggacagat caacttttcc aaacgatcca atcaaataac gggggtgtaa
     3661 atacagctac ttctacacta cctgcattgg cttcagggca atattatttt gattatcgct
     3721 tggtggacga tgtacttact aacatgtatc cgtcttttgc gtacaaatgg cagtctgaac
     3781 aagctggaat caccactctt cctcaaataa cagtggctaa ttttccatct gtgagtgcag
     3841 atagtcagct aaaaaacctt agcagacaac ctgagacagg agatcccctg tctgctctat
     3901 caggagatac gattcaagaa aatgtgacat tcacgttaac aaaatctggg gatgcgataa
     3961 ccgacaaaaa aatcacgatc tcacttcctg aaaatactac ttatatcaat ggttccttaa
     4021 aattaaatgg tacaactatc gcagataccg gcattcaaca aggagtttct gtcccagcag
     4081 atctattgag caaaatagga gataccattc atcttactta caactatcaa ttaaatactg
     4141 tagatacttc tttacaatcc gtcagcatac tgacaaaggc agctgtactt tccagcaata
     4201 ttacattagc agatggtgct aagcttccta acccagttgt ccaaacatct gctaaaacaa
     4261 ttcttgtccc caaacaagaa ttaacattag ttaatgtacc agatgatttt acatttggta
     4321 acgatctccc caaaccgttg aagacaagtt attatgaatc aaaaggcgat ttttcttttg
     4381 atgtgcggga tactcggctt ccaagcacgt ctccttggca actgacaggt acattgactt
     4441 ctttatttaa gaatgatcaa ggtcaagaat taagcggaac aaaattatac ttcaatcatt
     4501 ctggatcaaa gcaattgatt cagcaaggtc aaaacacgtt gatttatgag agtgatggaa
     4561 cagctaaagg agaggttctc gttgactttc ccgataccga tggtttactc ttagaggtaa
     4621 atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg catggtcaca tgggaactga
     4681 cagctgggcc aaccagctaa gtgattagaa tttgtatgga tctgtgataa ctgtcacaga
     4741 tcctcttttt caaggatgag aaggaaactt ctgctgttaa aatcagaaat gcttgaagga
     4801 gtggacttgg aacttttcag tttgcttgaa aaaaatgaac aacggcaaat caaacttttc
     4861 caacttttat tagaggaaaa aggagaggtt tccgttaata acgtacttac ccgcttatca
     4921 gttgatcggg ctaccttgaa agaagatctg ttttatttac aagctaactt ggaaaatatc
     4981 aaacaattgg aaattgttat ccaagaaggg aatattttat taatcagaca tggaaatata
     5041 gcagcagtcg atgtttatta tcattgttac ttgcgctatt ctacaaaata tcagatactt
     5101 ctttatctgt tggaaaatgg ctcatttgat cggcagaaac ttgtcatgga acttaatatc
     5161 agttcggcta ctttgtctcg cagagtgaag gaattaaatg attctttaaa tgagtttcat
     5221 ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc aaattagtta tttttatttc
     5281 caattatttt ggtttggacg tccctatctt gaaaacaaaa aagaatacag tgatttatca
     5341 ggaaacactt ttttagatct gctgcaaaaa gagtttagtt ttgctattac agaagaagga
     5401 cgattgaaat ttgaaatatg gctttccatt atgaagaagc gaatgcgctg tacgaaaaaa
     5461 tccaaaccag attatagcaa tcttcctgtt ggattgtttc aaacgacaca atttttattg
     5521 gacctccata catttttgac ccgttacttc tttcaatatg cctttccttg gaatcaaaat
     5581 gaaacgttgg ttttttatct tttcatgatc agtaacttta cattagatac acataactcg
     5641 catgtccagg agtttttgat aagcgaaaaa tacaaagacg acgtagtagc tcaattgaat
     5701 cgaacgttta aacaatcgat agaagaatat ttctcttttt atgtttttcc agaagagatt
     5761 tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc attttgccta ttttagagga
     5821 tggatgaata tttttggcca agaaggatta aaacagcggt taactgaagt agaagataaa
     5881 caattcctgg ttttatgtga aagattggtc agacaaacat ttgctattgt tgattttact
     5941 gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc gctatgcatc tattttacgg
     6001 acgctgatca agcagttaga attacgtctg caaattgctt atgatcttcc ttatgaaagg
     6061 ctaatggcgg atatcgtgaa agaaatcatc caggaacaat tgctgaatac gatcaaatat
     6121 gatttagtcg aatacgagaa agaaaagcag tatgatatca ttttgactag ccaattgaaa
     6181 gagtacccgt cacagaagtc cgctaaagtt tttgtattca ctagcaacga atataattat
     6241 gatttcccct atttgaacca atttttaaaa gaatgttatt tagaaaaatt gaatttaagg
     6301 atgaataaaa catgaaaatc gtgatcattg gtgcttccca tgaaggcatc acagctgccc
     6361 taaatctaag aaaacttcaa ccgaaagcag aagttttact gatcgatgaa gatcataaag
     6421 atggactagg ctatgtttca aatggaatca acctttattt aaaaggaaag atacgtagtt
     6481 tagctgaagt tgcccataac atgcggactc ttcaagaaag tggggcaaaa ctgatcaccg
     6541 aatggagagt aactgaactt gatccagaca aacatcagct catcctcgca tccaaagaag
     6601 gaaaaaacga gaccataacc tatgataaat taatcgtagc gacaggttcc tctcctgtca
     6661 ccttgtataa acaaattgaa gctgaaaatg tctacaccta taaaaatcta gtgcaatcta
     6721 agcaagtact tgccgcttta aaagaagcaa aagaagtagt gatcttcggt gctggttaca
     6781 ttggattgga attagcagat gctttgagaa ataaaggata cattattcat ctagttgatt
     6841 atatgccgaa tgttttatca agatatttcg ataaggatat gatcaacagt tttcaaaatc
     6901 agctgcaaac taagcaaata aatttttatc caaatgaatt cttgatcgat tggaaaaaat
     6961 cagaagagaa agtagtatcc gtacaacttc tgtctcaaac aataaaggca gatatggtca
     7021 ttttttctgc ccaaactcgt cctaatacga cattgttaaa agaaaaagtg gctttgtatg
     7081 aggatgagac tgtgatagtc aatgaatatc tgcagacaag tgatccggac atctatgcga
     7141 ttggagatat cgtcccagtc agttttgaca aaaataaacg gcatctcttt ttacctttag
     7201 ttactcgagc tgtacatatg gcaagagcag tagcgctaac tttgagtggt cagccaaccg
     7261 cttacgacct cagacaaaag ataacagcag cagtcattat agactacttt ttaggaactg
     7321 tagggctgac agaagatgaa gcgccattct tggaacaaaa taccggttcg tgttcaggag
     7381 aatttgatct atttccacaa tacgacgaag aaaacaaaac agtaaatgca aagctgatct
     7441 accatccgga tacattagaa ataattggcg gacagctgat aagccaagaa ttcttattat
     7501 cagatttgaa cctgcttgcg gatatcgtga aacacaaaac tacgattcca cagctagcag
     7561 ttgaggattt tggatttctg tctgaataca cctctcgttt tcactacctc aatgagttag
     7621 cttttaaagt actagctaag agagcaaata gcaatagagt agataaaaga ctgtgagaaa
     7681 tatcacggtc ttttattgtg tggggatata ataaatatta atcaa
//
LOCUS       DAZKNM010000127         7720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000127 DAZKNM010000000
VERSION     DAZKNM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.129"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..711)
                     /locus_tag="ACG8M9_002172"
     CDS             complement(145..711)
                     /locus_tag="ACG8M9_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0378896.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(711..1667)
                     /locus_tag="ACG8M9_002173"
     CDS             complement(711..1667)
                     /locus_tag="ACG8M9_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0378897.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1667..2323)
                     /locus_tag="ACG8M9_002174"
     CDS             complement(1667..2323)
                     /locus_tag="ACG8M9_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0378898.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2693..4711
                     /locus_tag="ACG8M9_002175"
     CDS             2693..4711
                     /locus_tag="ACG8M9_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0378899.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4781..4987
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5138..7552
                     /gene="leuS"
                     /locus_tag="ACG8M9_002176"
     CDS             5138..7552
                     /gene="leuS"
                     /locus_tag="ACG8M9_002176"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0378900.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         2554 a         1477 c         1571 g         2118 t
ORIGIN      
        1 ctcatgtttt tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa
       61 taaagaggtt atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata
      121 tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg
      181 ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt
      241 ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac
      301 gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg
      361 tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa
      421 aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg
      481 tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc
      541 gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc
      601 tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg
      661 caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc
      721 acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca
      781 attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac
      841 ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg
      901 caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc
      961 cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct
     1021 gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat
     1081 ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct
     1141 aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt
     1201 tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact
     1261 acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa
     1321 ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa
     1381 ttttggaaat aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt
     1441 tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca
     1501 gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca
     1561 aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa
     1621 ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct
     1681 ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca
     1741 gccaagaaag actgagtaag taaccgccga tgatatcagt aggaaaatga acgcccagat
     1801 aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc
     1861 gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca
     1921 tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca
     1981 atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta
     2041 caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata
     2101 aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga
     2161 gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga
     2221 aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa
     2281 gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa
     2341 gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
     2401 tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag
     2461 acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc
     2521 ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg
     2581 gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt
     2641 aagagaagtt ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa
     2701 cattgcaaga aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg
     2761 aggggcagca atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca
     2821 gccggttcct gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc
     2881 ggcatttatc acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata
     2941 tgcatcagtg atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc
     3001 tcaagcacct tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta
     3061 catggatacg cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca
     3121 atatccttct tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt
     3181 tggcaacggt tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc
     3241 gacagcttgt ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa
     3301 tatcatcgca acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg
     3361 tggagcgact acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa
     3421 ttctggtaat acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga
     3481 ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa
     3541 caacatccaa aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga
     3601 aaatggctct tcgacgaata attccggtaa caataccaat tcttcaggaa atgctggtac
     3661 atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg
     3721 gaagatctca aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa
     3781 gaacaatttc gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc
     3841 tggagcaaat actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag
     3901 ttcttctaat acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag
     3961 cgtctcaaat aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa
     4021 taactttatc tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac
     4081 gtcaggtaat acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag
     4141 cagttcgtct caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa
     4201 taaaaacggc atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat
     4261 ttatccagga cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc
     4321 aactacttct gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc
     4381 cactggtaat acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa
     4441 acatcatatc acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta
     4501 tccaggacag caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt
     4561 tactggttcg aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag
     4621 tcatggaatt acaatggctc aactaatcga atggaacaat attaagaaca actttattta
     4681 tccaggtcaa acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa
     4741 ttccacaaaa atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag
     4801 tagtaaaaag actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt
     4861 tgaactcgcc tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc
     4921 gttacaacgt tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt
     4981 cctataaaaa gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa
     5041 acaaagcaaa aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat
     5101 agaaacaagt gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat
     5161 tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc
     5221 tgaaaagcca aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca
     5281 tgtagggcat cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca
     5341 aggatacaac gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata
     5401 tgcgctagat acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg
     5461 acgccaaatc aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga
     5521 tccggagtat tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc
     5581 ttatgaagca gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga
     5641 agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg
     5701 ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt
     5761 tgattggcca gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc
     5821 aaatattgaa tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc
     5881 agatactcta ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga
     5941 aattacgacc cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa
     6001 atcagatcta aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta
     6061 tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc
     6121 ttcatacggc acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt
     6181 cgctcaaact ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc
     6241 tgcttacaca gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca
     6301 agaagcaatc gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt
     6361 cagctatcgc ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc
     6421 aatcatccat tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg
     6481 gttgcctaag acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat
     6541 cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa
     6601 tacaatgcca caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa
     6661 caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat
     6721 tggtggagca gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt
     6781 atacgatatc ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat
     6841 gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga
     6901 tgatgtggtc gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc
     6961 attagatgct tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga
     7021 tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt
     7081 gaatgatggc aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta
     7141 tgaaaatctt catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta
     7201 taaagtggac gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat
     7261 tgctcctcac atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta
     7321 cgcaccatgg ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt
     7381 ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga
     7441 gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt
     7501 cagaaaagtt attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct
     7561 ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc
     7621 tgtctcagat tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt
     7681 tcatgttctc aaacacgttc aatgtctttt tcttctgaat
//
LOCUS       DAZKNM010000128         7699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000128 DAZKNM010000000
VERSION     DAZKNM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.130"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..130)
                     /locus_tag="ACG8M9_002177"
     tRNA            complement(58..130)
                     /locus_tag="ACG8M9_002177"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95..97),aa:Lys,seq:ctt)
     gene            511..1080
                     /locus_tag="ACG8M9_002178"
     CDS             511..1080
                     /locus_tag="ACG8M9_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0378901.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1067..1927
                     /locus_tag="ACG8M9_002179"
     CDS             1067..1927
                     /locus_tag="ACG8M9_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0378902.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2117..2821
                     /gene="yycF"
                     /locus_tag="ACG8M9_002180"
     CDS             2117..2821
                     /gene="yycF"
                     /locus_tag="ACG8M9_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0378903.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2826..4661
                     /gene="walK"
                     /locus_tag="ACG8M9_002181"
     CDS             2826..4661
                     /gene="walK"
                     /locus_tag="ACG8M9_002181"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0378904.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4658..5974
                     /gene="yycH"
                     /locus_tag="ACG8M9_002182"
     CDS             4658..5974
                     /gene="yycH"
                     /locus_tag="ACG8M9_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0378905.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5975..6844
                     /locus_tag="ACG8M9_002183"
     CDS             5975..6844
                     /locus_tag="ACG8M9_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0378906.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6899..>7699
                     /locus_tag="ACG8M9_002184"
     CDS             6899..>7699
                     /locus_tag="ACG8M9_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0378907.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELF"
BASE COUNT         2612 a         1312 c         1687 g         2088 t
ORIGIN      
        1 atgttttcga aatgctttcg caccaacatt tacctgttcc gtgggtttgg cacccaatgg
       61 gccgtgaggg gctcgaaccc gcgacccgct gattaagagt cagctgctct accaactgag
      121 ctaacggccc gtacaaaaag tacttaataa tagtagcact aagaatttta aaatgcaaga
      181 ctacatttaa agaaaatcag aaaaatcttt acttccttat aggaaatcat tcatagatta
      241 ttcacatttg ctcgctatta tataagcata ccaacaaaca aagttcttgc aagagaacaa
      301 accctaacac ttttttcatt ttttactcct ttcccgccgt ctccccggcg ggtctcctgt
      361 tcttaacgat aaaagacaag aaggcttagc cttcttgttt tttgttttca tggaatcccg
      421 ctaatcaaat cacctagtta aagcaactag attgaatgtt tatcataagt ctgatatcat
      481 gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa tgcgtcagca attaatggag
      541 tgggggaagg aactagaaac attccgtctg cctcactggg aagaactccc tgacttagaa
      601 ctttatatgg accaagtcag aacgttagtt gatcgttatc tttcacctgt gatacaagga
      661 gaaaagcatc cattgcttac ctcttctatg gtaaacaatt acgttaaact aggtttgata
      721 ccagcaccag ttaaaaaaag atataacaaa gaacacgtag catttttact tgcaatcact
      781 acgttgaaac aagtattgac tattcctgaa atcaaagaag ggatattgtt ccaaggaaaa
      841 acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg aacaagaagc agctgtgtgg
      901 atggtcagcc aattagcaca aggtaaatct catccgcaaa aattcgacca ggcaacccct
      961 gtagaatata ttgctgtaag agcggcaact ttatcgtttg caatgaaact tcttgcggaa
     1021 aaaacgattg ttctagaaac cgaatattta aaagaggaga aaacaaatga aaaacaataa
     1081 aatcgcaatt ttagtagatt caggtacgga tgttccagaa gaattgataa aaaaatacga
     1141 gatgtatatg atcccattga aaattattta taaagatcag gtatatacag ataaaattga
     1201 cattacccct gaagagatct atcaacgttt gccagaggaa atccctggta catctttgcc
     1261 ggatggtgag tcgatcacag aaatctttga gcaaatcaaa aaagacggtt atgaaaaggt
     1321 acttgcggta acaatttcca gtggattgag cgggacatat aatgtggttc gtttaattgc
     1381 acaacagcaa gaagaattgg aagcatatgt actagatacg aaaaatattg gtattggtgc
     1441 aggtttttct gcgatccaag cagcaaaatg gctggaagaa ggagtagaat gggatcaact
     1501 gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt ttctttaatg ttgcgacatt
     1561 ggaatatctt caaaaaggtg gacggatcgg tttagttgct tctattgtag gaaccgcttt
     1621 aaagcttaac ccaatcatct catgtaatga agaagggatt tattacacag taggaaaagc
     1681 taggggacgt aaaaaaagtt tggataaaac ggttgcttac gtcaaagaaa aaatcggtaa
     1741 tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg aaagaagaag caattgaaat
     1801 ggaagcgaga cttaaaagag agttcccaca agcaaaccaa atctactttg gccagatttc
     1861 tccggcatta gtagtccaca ctggtccggg attattgggt gtcggtgtac aagttttaga
     1921 aggataaatc aaaaaaagga aacactgagg tgaaaatcag tgttttcctt ttttattgaa
     1981 gttagaacaa aaaataataa agtattttgc actttcattt taattttctc ttttgtttta
     2041 taggcaaaga tgaaacaaaa gcagaaatac ggtataatga gaaggtatga caataatata
     2101 taagaggtgg ctctttatga aaaaagtttt agttgtcgat gatgagaagc cgatttcgga
     2161 tatcgtcaaa ttcaatttag ccaaagaagg ctatgacgtt tatactgcat atgatgggga
     2221 agaagccttg gaaaaagttg ctgaggtaga acctgatttg attttacttg atttgatgct
     2281 acctaaaatg gacggcttag aagttgctcg tgaggtacgc aaaacttatg atatgccgat
     2341 cattatggta acagccaaag attctgaaat tgataaagtc ctaggcttag agttaggcgc
     2401 cgatgattac gtgaccaagc cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa
     2461 tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag gcagcacctg cggaattgac
     2521 gattggtgat ttgacgatcc atccagaagc ttacatggtg acgaaacgag gagaaacaat
     2581 cgagttaaca catcgtgaat tcgaactact cttttacttg gcgaagcacc ttggacaagt
     2641 gatgacaaga gaacatttat tacaaacggt ttggggttat gattattttg gagatgtacg
     2701 aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc gaagataatc caagccatcc
     2761 aaattattta gtgacacgtc gtggtgtcgg ttactatttg cgtaacccag aacaggagta
     2821 attggatgaa aagaaaagtc cgcttttttc gatcagtcaa ttttaagatc gctattacct
     2881 ttattctgat tttattgatc tcgatcgaaa ttattggtgc ctactttata cgtggtttag
     2941 aacgttccac gatcaataca tttattaaag atatgaatca aacggtggaa tcattagcaa
     3001 caacaatcag cccggaattg aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga
     3061 atatcaaaag atttattgaa aacagtgcga ctagcgatat catcgaaata cgggtcgttg
     3121 acgaaaaagg gatcatccga ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga
     3181 aaaatgatta tgttgatatc aatgatttca cgactaaacg ttatgttgct ttagataacg
     3241 acgataaacg ggtgaatatc aatgttcaac cgatcctgtc cccgaccggc gatgccgtta
     3301 tcggggctct ttacgtcaaa agtaatatcg aacaaaaata ttcggaaatc aataacacag
     3361 cagtcatttt ctttactgcc tcactaattg cggcatttat ttcaatgatc gtatctgttt
     3421 tagttgctcg ttcgattaca cagccgatcg gtgaaatgag agaacaagcc attcgaatcg
     3481 ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca agatgaactt ggacagctgg
     3541 ctgatacctt caatcagtta gctgaacgta ttgaagaagc tcaagataca atggaagctg
     3601 aacgcaatcg tttggatagc gtgctatccc atatgacaga cggcgtcatt gcgacagatc
     3661 gcaggggtaa agtcattacg atcaatgata tggctgtttc cttgctagat atcaaaaacg
     3721 aggaagcaat cggacaatcg attttgacac ttttagatat cgaagaagaa tatacgttgc
     3781 ggaaattatt agaagatcca aacgaaatgg tacttgaccg ttcaaccagt acattggaaa
     3841 gtgatcagat gatcttgcga gtagactttt ctatgatccg tcgcgaatca ggttttatca
     3901 gcggattagt agctgtcttg catgatgtga cagaacaaga aaaaacagaa cgggaacgtc
     3961 gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt
     4021 atatcgaagc attgagcgaa ggtgcgtgga aagacgagga agtagcacca aattttttga
     4081 aggtaacgct agatgaaaca gaccgaatga ttcgaatgat caatgacctc ttaaatctat
     4141 ctcgcatgga tacaggtaat acccagcttc aattagaata tgtcaatttc aacgagttag
     4201 tgaattttgt tcttgaccgg tttgatatga tggtgggaaa tcaggaaaaa aattataaga
     4261 tccgtcgtga atttacgcaa agagatcttt gggtcgaagt agatacagat aaaatcatcc
     4321 aagtagtcga taatatcatg aataacgcga tcaagtattc accagatggg ggaacgatta
     4381 cttgtcgctt attagaaacg cataataacg tgattttaag tatcaccgat caaggattgg
     4441 ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt ctatcgagta gataaagcac
     4501 gtgcaagagc acaaggtggg acaggacttg gattagctat ttctagagaa gtgattaagg
     4561 cgcatcgagg cgctatttgg gcagagagca aggaaggaaa agggtcgact ttttatatct
     4621 cgctgccata tgaaccttat gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt
     4681 tcgtatagga ctcattttga tggttgcgtt aagcatatac ttctcttatg caatctggct
     4741 gagcccagca ggaaaaagtt caatcaacgt agatgagaca aacactcaaa tgatcgagtc
     4801 tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt catgttacat ggcaccattc
     4861 gggaatgatc caagaaacca acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga
     4921 taaagcaaga tttggaaagc tttctgaagt agtttcagga gacaaatcac agtttgaaca
     4981 ggtgaaaaat ctgaactctg gttttgagtt ggcctataat gcgccttttt tgctaagaga
     5041 atacaaggaa gcatttgatt tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa
     5101 tatgtatttt actagaatac aattcgatca aggaaataaa aaaatccgat ttttgaatta
     5161 ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat tggacagata tcaataaaga
     5221 attaacagca aaagacacaa aatggacaga aatgacaaca gaaccatcga cgatcgacac
     5281 gcaatatgct acgaaacatt cgatcaaact gaagaagtat agttatatcc tgtcacagca
     5341 accctataca ctttttagaa atgctttttt ccagaaacca gatagcgtga aaaataatga
     5401 tgaaagcaat gaattgattt tttacggtgg gaatgaaaca atgtctatcc attcggaaac
     5461 gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc
     5521 tgaaagtttc ccctatatta gcaaattagg cagttcttta gggaacctca gatattttga
     5581 ccgcagtgac aatacgatcg attatcggat attcgtggaa ggcttcccgg tttttgggac
     5641 agacagcaga ggacagattg gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt
     5701 gaatatccaa accagtttga atacgattca agtgccgata ccttcagacg aagaagtaga
     5761 acttgcaagt acgaatgaaa tcatccagca gttaaaagct aagggagcag atagcaacaa
     5821 gattggttca gtgatcgttg gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt
     5881 tgatctctta cctgaatggt atgtgaaata taaagataac tggtattcag ctaacgacct
     5941 tctggaaaaa ttacctggat tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat
     6001 atttttcgtc gcttttttag ggttgaatgt gtttttattc agcagttatc atgacgcggt
     6061 ataccaagaa aacaatgtca ttcgctctaa tcagactgaa tcaatccaac aacgtctggc
     6121 tagcgacgat atcacttaca cggggaagat ttcgtctgag aacaagcaag gctattatct
     6181 tagcgcggag cagaccaatc ttagcacttc attagcgaat tatcgcaagg ctggtaaaag
     6241 cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt ttgacgaaat ctttatccag
     6301 ttcagatgct gaaaagaacg tgatcgatcc agataaaatt agtagttcct tggaagattt
     6361 tttatctaac aaagacaata ttttattcgg taatgattat gtgtacacga aaaacttttc
     6421 tcatttagaa gaagaaccac tagaaatcca agcaagccaa gagtatgaag gcatcccatt
     6481 caatgatgat acagcaaaaa tcgttctttc tttagaaaaa aacgataatg attatagcat
     6541 cacgaaatac atgcagacgc atttatctga tatcgagcag ctgcgcgaaa agacggaact
     6601 gtatacggaa gaggaagcaa tcaatacatt gtacatcaat aacaaaatac caagaggatc
     6661 aaaaatttta tggcgtcagc tagcttattc ctgtattctg aaagtccggg aaaaaaatgt
     6721 gtatgttcct gtttggcatg tagcgatcga aacaagcgat aaagtggtcc aaattgagag
     6781 cgtcaacgct ttcagcaata cgatcgtgac aaacaatact gtaccaaaag tggaagatca
     6841 ataaaaaagc gagtataatg aaaaaagttt acaaagtaag aagggaagta ggagtcaaat
     6901 ggctgaagaa aaaccagctt ttaaaatcag tatcctagca agtggaagtt cgggtaattc
     6961 actttatatc gaaagtgaaa aaaaacggct tttggtcgat gcaggactta gcggtaaaaa
     7021 aatcacttcc ttgatggctc aaattgatcg gacaccggac caattagatg gaatacttgt
     7081 gacgcatgaa catcgagatc atatacatgg cgtgggtgtc ttggctcgta agtatcattt
     7141 ggatgttttt gccaatcagc agacttggga agcgatggaa ccactgattg gaaatgtacc
     7201 agtggaacaa aaacatctat ttgagatggg gaaagtcaga acattcggcg atatcgatat
     7261 tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa ttttatcgtt tccataaaga
     7321 aaatcggtca ttcgtcatgt tgacggacac cggatattgc agtgattata tgcgaggtgt
     7381 cattgaaaat gccgatggtt atttgatgga aagtaatcat gatcttgaga tgttacgcat
     7441 gggaccttat ccgtggaatt taaaacagcg tattttagga gatagaggac atctttccaa
     7501 cgaagacggt gcacttgtca tgactgatgt gatcggtaat cggaccaaac gaatctatct
     7561 tggccatctt agcaaggaaa acaatatgaa agaacttgct catttaacaa tggaaaatgt
     7621 tttgaaagaa aaagattttg gcgttggaca tgattttgaa atctatgata cagatccaga
     7681 tgaagcaact gagctattt
//
LOCUS       DAZKNM010000129         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000129 DAZKNM010000000
VERSION     DAZKNM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.131"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8M9_002185"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8M9_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0378908.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8M9_002186"
     CDS             1483..1935
                     /locus_tag="ACG8M9_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378909.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8M9_002187"
     CDS             1949..2638
                     /locus_tag="ACG8M9_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378910.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8M9_002188"
     CDS             2763..5114
                     /locus_tag="ACG8M9_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0378911.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8M9_002189"
     CDS             5194..5589
                     /locus_tag="ACG8M9_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378912.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8M9_002190"
     CDS             5914..6531
                     /locus_tag="ACG8M9_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0378913.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8M9_002191"
     CDS             6572..6871
                     /locus_tag="ACG8M9_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0378914.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8M9_002192"
     CDS             6905..7072
                     /locus_tag="ACG8M9_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0378915.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8M9_002193"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8M9_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0378916.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKNM010000130         7283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000130 DAZKNM010000000
VERSION     DAZKNM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.132"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..950
                     /locus_tag="ACG8M9_002194"
     CDS             <1..950
                     /locus_tag="ACG8M9_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HFQ0378917.1"
                     /translation="KEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLGKEAELDQFSE
                     IQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIYEFTQENGLVE
                     KETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKNEIETCVNEIR
                     KLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRD
                     VEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDE
                     IDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            953..1849
                     /locus_tag="ACG8M9_002195"
     CDS             953..1849
                     /locus_tag="ACG8M9_002195"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0378918.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1899..2288
                     /locus_tag="ACG8M9_002196"
     CDS             1899..2288
                     /locus_tag="ACG8M9_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0378919.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            2275..3774
                     /locus_tag="ACG8M9_002197"
                     /pseudo
     CDS             2275..3774
                     /locus_tag="ACG8M9_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4522..6348
                     /gene="ltrA"
                     /locus_tag="ACG8M9_002198"
     CDS             4522..6348
                     /gene="ltrA"
                     /locus_tag="ACG8M9_002198"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0378920.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            6461..7283
                     /locus_tag="ACG8M9_002199"
                     /pseudo
     CDS             6461..7283
                     /locus_tag="ACG8M9_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         2561 a         1167 c         1548 g         2007 t
ORIGIN      
        1 caaaagaagt tgtagtcgaa ttcagcgata atttaacttc aattattgga ggaagaggaa
       61 caggcaaatc ttgtattaca agattcttgg tctatgtcct tggaaaggaa gcagaacttg
      121 atcaattttc tgaaatacaa agtgattatc agaactttgc acaaattgaa cataatggct
      181 ctggaatatt tttgaaggat acaattgtga aactgaatat tttctataag gggacaaaat
      241 atcaaataat aagaacacag gatagacatt ctatttatga atttacacaa gagaatggtc
      301 tggttgaaaa agaaacagaa cggttgagaa tgatttccga taaggtgagt atatactctc
      361 aaaaacaaat ttatgaaatt tcaaaaaatc aaactagtat actagaatta ctagatggat
      421 ataactctga tttgatttca gagtataaaa atgagattga aacatgtgta aacgaaataa
      481 gaaaattaaa ttgggatatt gtttctgtga aaaaagaaat acaagataag gcgaaggtag
      541 agcttgaaat tgaagattta aaaaaacaag tagaaaagct atctcataag agttataaag
      601 atgtttatga tgagtattca aaagagacag atatttatag ggaactgaaa cgtgatgtgg
      661 aggcacttaa ggaaattccc aaaaatctaa ctgatagtgt tgacaaaatt gactttggca
      721 atggtataaa agttacagac gaaattgatg aacaccgcgg agaattgatt aagaatttag
      781 tgaccaatcg ggctaagatt caaacaataa ttaatgaaat gtcagatgaa attgatagtt
      841 atagagcaga gctgaataaa tcagattgga aagttaacta taatgaggtt tcaaagaggt
      901 atcaaagagg tatcaaaaac ttagtgaaga gttatctcaa gatgaattaa aaatgttaca
      961 aaaccttgat tctttatatc aatctttatc aaaaactgaa gagcgattgg aagaaattat
     1021 tcaacaagaa ataaagctta aagattacca agttgaggtt gactcagcaa tgtcaagaat
     1081 gaataaatca tatcaaaatt tctattctga gagaagtagt tcctcgcgcg aaattttttc
     1141 tgatattaca ggaatagagg ctactattca accaaatagg gatttcaata attatgttaa
     1201 agagctcaga gcttgtatta ataaggagga cacttttgac agtgagtttg gatctcttat
     1261 tgataagctg acaaaaaatt ccattactaa tcaagatata tataatgaca tacttgatat
     1321 aagagttagt ggggaaagta gtattttttc agataaaaaa ttaatcaatt ctataagaaa
     1381 attgaatgat gcacaagttc ttgaattaca aacccttaag ccaaatgatt tagtagttat
     1441 ccgacttgaa ctaaatggaa aaaaagttag tttatcgaat gctagtgctg gtcaaaagac
     1501 atctgcaatt ctttctatga ttttggcgta tggaaattca ccactcatat tggatcagcc
     1561 tgaagatgat ttggatagtc aattaattaa taacttaatc gtaagaagta tcattacaaa
     1621 aaaggaaaac agacaaattt taattgtaac tcataatgca aatatcccag ttaatggtga
     1681 ttcagaatgg ttgattagca tgggagattc aaaagaaata tcggctgatc agtgcgggtc
     1741 ggtagatgaa cccaatatca agccaagaat atgtactgtg atggaaggtg gcgaggatgc
     1801 ttttacaaat agagcgaaac gctatggttt taaacaaatt gtagaataac actagagggt
     1861 gagacatatt attatgtccg ccctttttag gaggaaaaat gaaaaaaata aaaagctata
     1921 ccagtatctg ggcagttgaa aaagtcattt acgcaatcaa tgattttcag ttgccttttc
     1981 ctgtgacttt taaccaaatg gcttggttcg ttttatcttt actgtttgtc atcgtatttg
     2041 cccatgtgcc tccactatct atgatagagg aggtcttttt aaaatactta ggcataccgg
     2101 tggctgtcac ttggtttatg tcgcaaaaga cgtttgatgg caagaaacca ctgggatttt
     2161 tacgatcctt tatcagctat cacttgcgat ttaaggtgac ttttgcagga aagaaagtca
     2221 aagaacagaa gaaacgatgg gatgaaccca gtactcttgt aaggagtgtg aactatgtat
     2281 ccgattaagt atattgaaaa taatctcgtc tttaaccaag aaggcgagtg ttttgcctac
     2341 tatgaattag tgccttacaa ttactccttt ttgtcacccg aacagaaatt tcaagtgcat
     2401 gataactttc gccaactaat tgcgcaaaat cgtgaaggca agatccatgc gttacaaatt
     2461 gccacagaaa gtagtattcg cgccacacaa gaacgatcaa aaaaagaaat taccggtaga
     2521 ctcaaagaag tagcgaaaca acgaattgat atacagacag atgcattagt atccatgatt
     2581 ggcgatagtc agattgatta tcgctttttt attggcttta aactaattgc gacagatgaa
     2641 gaagtcaatc taaagagtct caagaaatct ttcttttctg ggcttcaaga atttgtctat
     2701 ggagtgaatc atcatttgat gggcgatttt gtttccttat ccaatgaaga aattcgctgc
     2761 tattctaagc ttgaaaaatt aatggaaagc aaattggctc gaagatttaa agtcaggcga
     2821 gtcacaccga gtgacctgac gtatttgatt gaacatattt atggcgaaaa agggataccg
     2881 tttgaagaat acgaatttca gctaccaaag aaaaagctaa aatcagaaac actggtgaaa
     2941 cgttatgact tattacgtcc aaatcgttgt ttaattgaag aaaaaccaag gtgcttacgg
     3001 atggaacatg agaatcatga gtcgtacgta gcgtatttaa caattaatac aattgtgggg
     3061 gagatggaat ttccttcctc agaacttttc tattaccagc aacaacaatt tacattccca
     3121 atcgacacaa gtatgaatgt agagattgtg acgaataaaa aggctttagc taccgttcgt
     3181 aataagaaaa aagaactgaa agatttagac aatcatgctt accaatctga caatgaaacg
     3241 aattccaatg tcttagatgc tttggattcc gtggatgaat tggaaaccac cttggatcag
     3301 tcaaaagaat ctatgtacaa actgagttat gtggttcgag tgagtgccga atcagtggat
     3361 gaactaaagc gacgctgtga tgaagtgctc gatttttatg atgataccaa tgtaaaacta
     3421 gtccggccct ttggcgatat gatggggcta catgaagaat tcttaccctt aagtaaacga
     3481 tatatgaacg actatattca gtatgttacg tctgattttt tggcggggct tggttttggg
     3541 gctactcaga tgctaggaga attagaaggg atttactttg gctacaatgt ggatacaggt
     3601 cgtaatgttt acctgaaacc ggcattggct tcacaaggag tcaagggttc agtaaccaat
     3661 gcgctggccg ccgctttcct gggttctctt ggtggaggta aatcctttag taacaatctc
     3721 ttagtctact atgctgtttt gttcggtggt caagctgtga ttgtcgatcc aaaagggggt
     3781 gcgaaacgtt cctacttgaa aagagtaggc acagcacttc attaaatcaa tatttaatgt
     3841 gtgttggaga actaaacaca cgaatagtcg aatgacgggg taacgccctg aaaggctact
     3901 ttaatactct gactggcaga aacttgagta aacaaggctc tgtcagaagc tcggtgaagt
     3961 cggcagacag acaccgtaac agataatgct gacgaaagat tcccagaggt gggcgttgtg
     4021 tcctatatgc cggaagtcta taaaatatct atggttagaa tgtagatgag aggtctactg
     4081 acgaatcctt gaatgtacag gtctatatcg gggctggata gaaatgtcca gtgacacaaa
     4141 cagtgtcgct actgtgggtg agtaagactc cgcatttatg aaaatccata taacgttaaa
     4201 ggcgttatca aggtagcagg cttatagagg aaacctaagg atatatgtac agatagtgtg
     4261 tttggaacgt tgaaagctga caacatggag attttactgt ctgtgaagtg ttcacatcag
     4321 aaagttattg tgagattagc aatgatataa ggtgtgttaa tgtcagtgag agtggtggca
     4381 cagtacctat gaaaccgtga taacaagcgg tggagggata gccactagtc atatgaagaa
     4441 tagaattact aagaaaaatg gtatgggttc gagtaagact aagagatgta cttgctcctg
     4501 caaacaagga gggtacagac catgctaaag aaaaacaagc tccgctataa cgagtattat
     4561 gatatgcaac atatttatga cgagttatac gcacagagca agaacggtaa taacttttat
     4621 aagttacttg aaattattgg ttcggaacaa aatatttgtt tagcttaccg taatctgaaa
     4681 tcaaacagtg gcagtaaaac ggctggaacg gatggaatga cgatagacga tataaaacaa
     4741 ctcagcaatg ctgagattgt tgctaccgtt cgggaaagcc ttagcaacta ccgccccaaa
     4801 tcagtcagac gtgttttcat tccaaaagct gggagcgata aaatgcgtcc attggggatt
     4861 ccgtgtatct gggacaggct ggtacaacaa tgtatcctgc aagtcctaga gccgatatgc
     4921 gaaccaaagt tccataatca ttcctatgga ttccgtgcga atcgaagtgc ccaccatgca
     4981 gtcagcagag taacgacact gataaattta tcaaaatatc attactgtgt tgacgtagac
     5041 attaaaggat ttttcgataa tgtcaatcat ggaaaactat taaaacaaat ctggacactt
     5101 ggtattcggg ataaaagatt gatttgcatt atcagcaaaa tgctgaaagc cgaaattgac
     5161 ggcgagggtg tcccagaaaa aggaacgcca caaggcggtc tgttaagtcc actgctctcc
     5221 ctcatagtcc ttaatgaatt agactggtgg gtaagcagtc agtgggaaac attccagccg
     5281 aagaaccgca gtaaaaatgg gtggcttcaa tacgcaaaga aatataccaa actgaaaagc
     5341 ggttttattg tacgttacgc tgatgatttt aaaatcatgt gttcaaccta tggagaagca
     5401 caaagatttt accacagtac cgtagatttt cttaataaaa ggctgaaatt agaaatcagt
     5461 ccagagaaat caaaggtagt aaatctgaag aaaaattcat cagattttct ggggttcaaa
     5521 atcaaagtca ttccaaaagg aagaacgaaa cacggttatg ttgccaaaac agatatgaac
     5581 caaaaagcat taaagaaagc gaaaacaaat ctaaagttaa aggtaaaaga tatagccaga
     5641 catacgactg gcttcaatat cagcagatat aatctgacag ttatcggtat gcagaattac
     5701 tactgcatag caactaacgt atataacaac ctgacagaag taagttacgc tctcttaccg
     5761 accatacgaa tccgtttaag gaacattgca aaatcagttc catttgaaag tacaagttca
     5821 gaatttcaga gcaggacaaa aggaatcaga ccaaaaacaa aaatagtgat gattgcggat
     5881 aatccgctat tgccgattca aggagtacaa cacaaaaatc ctatgaattt ctcgcaagat
     5941 atttgtaatt ttacaaagca agggagaaac aaggtgcatg aggacgttgt agttgtaaca
     6001 aaagaagaaa tccgagcgtt gttggaaaat gaaaaccctg ccgatagcgt agagtttaac
     6061 gacaatcgta tttcggcata tatcgcacaa caaggcaact gctatgtaat gaatcgtcga
     6121 ggaactccgt ccactctgat atgtattcac aaatcagaca aaggagataa ccttgacaga
     6181 tattctaatc tggcatttgt ggaaattccc attgcaaagg caatcctaac agaatcagca
     6241 gacgaagcaa aatcactgtt aaaaggttat gttcttaata gtcagcagaa gaaaaaactg
     6301 aacagaatac ggacgaatta cggataccag acaataactg ggaaataact tataaatcta
     6361 tttggatatg atggaacgcc gtatgaagcg aaagtttcat gtacggtgtg aagcggggga
     6421 aaacttggag ataacatcaa aaagttacct atcgctatag cgaggagggt ggaaagaaac
     6481 cttgccagaa attgcggatg aaatcagtat cctcaacttg acgagtaagt cagaaaatca
     6541 aggtctgctt gatccttatg tgattatgaa gaaaaagaaa gactcggaaa gtttagcaat
     6601 tgatattctg acttttttaa caggaatttc aagtcgagac ggggagaagt ttcctgtgtt
     6661 acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg gggttattac aagttattaa
     6721 cgaattgcga aaagacgggt caccaatcgc ggaaaatatc gccgatcata tcgagtcctt
     6781 taccgattat gattttgctc atttgctttt ctcagacggc tcgattaccc aatcgattag
     6841 tttagataaa cagctaaata tcattcaagt ggcggatttg gttttaccgg atgcagaaac
     6901 gacttttgaa gaatatacca ccatggaatt gcttagtgtt tcgatgctaa tcgtcatttc
     6961 cacttttgcc ttggacttta tccatagtaa ccgagaaatc ttcaaaattg tcgatttgga
     7021 tgaggcttgg agttttcttc aagtggctca aggaaaaacg ttatcgaata agcttgttcg
     7081 tgctggtcgg gccatgaatg ctggggtcta tttcgtcact caaaatagcg acgatttatt
     7141 agatgaacgt ttaaaaaata atatcgggtt gaagtttgct tttcgcagta ccgatattca
     7201 tgaaatcaag aaaaccttgg aattctttgg tttggatcag gaggatcaaa gcaatcaaaa
     7261 acgcctgcga gatttggaaa acg
//
LOCUS       DAZKNM010000131         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000131 DAZKNM010000000
VERSION     DAZKNM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.133"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8M9_002200"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8M9_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0378921.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8M9_002201"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8M9_002201"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0378922.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8M9_002202"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8M9_002202"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0378923.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8M9_002203"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8M9_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0378924.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8M9_002204"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8M9_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0378925.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG8M9_002205"
     CDS             complement(4433..5290)
                     /locus_tag="ACG8M9_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0378926.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8M9_002206"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8M9_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0378927.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAZKNM010000132         7072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000132 DAZKNM010000000
VERSION     DAZKNM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.134"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..826)
                     /gene="truA"
                     /locus_tag="ACG8M9_002207"
     CDS             complement(86..826)
                     /gene="truA"
                     /locus_tag="ACG8M9_002207"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0378928.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1017..1652)
                     /locus_tag="ACG8M9_002208"
     CDS             complement(1017..1652)
                     /locus_tag="ACG8M9_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0378929.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1799..3019)
                     /gene="sstT"
                     /locus_tag="ACG8M9_002209"
     CDS             complement(1799..3019)
                     /gene="sstT"
                     /locus_tag="ACG8M9_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0378930.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            3238..4236
                     /locus_tag="ACG8M9_002210"
     CDS             3238..4236
                     /locus_tag="ACG8M9_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378931.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4432..6267
                     /locus_tag="ACG8M9_002211"
     CDS             4432..6267
                     /locus_tag="ACG8M9_002211"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0378932.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6327..7043)
                     /locus_tag="ACG8M9_002212"
     CDS             complement(6327..7043)
                     /locus_tag="ACG8M9_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0378933.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT         2267 a         1386 c         1157 g         2262 t
ORIGIN      
        1 tatatttctt taatttgctt catacattat ttaacatcga tatttatact gtgaaacatg
       61 cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa aacagacctt
      121 gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt tcatcgattg
      181 tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata atccgcacca
      241 ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc tttcgttcga
      301 tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat gcagaaaaac
      361 caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg tccatatcca
      421 actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc acgttgttcc
      481 aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag cgctcaggca
      541 tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata aagtcgatca
      601 tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct cgcgcatggg
      661 tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc atctgatgca
      721 atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt tgccatcctg
      781 aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt tagtggctcc
      841 tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa atatagctcc
      901 ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt tcagaaaaac
      961 aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct caagttttac
     1021 tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa aaaactgatt
     1081 ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg aattcttgcc
     1141 agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta taagtggctt
     1201 acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa caataaaaaa
     1261 ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt ccacgaagat
     1321 tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc atagaacgcg
     1381 atggctgctg taactagggt agctaacaca cgagatgtac taacaaatgc agcatacaat
     1441 gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag aaacgctttt
     1501 cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg atctaattca
     1561 ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc taataataaa
     1621 aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca attacctatg
     1681 cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct atttacacga
     1741 acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata tctaacgttc
     1801 aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca cttcttttac
     1861 gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt aaggcagttt
     1921 ccattgaatc ctggataaca ccgataataa atccaatacc gacgatttgc atcgcgacat
     1981 cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa
     2041 cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact ttcaaaacaa
     2101 aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa atggtaatcg
     2161 cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa gactcttttg
     2221 tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca gaacttcggg
     2281 taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga tagggatttt
     2341 ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac agcatcgtac
     2401 cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca gtcgtagcaa
     2461 ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc acttgtacga
     2521 cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct gaacgtgctc
     2581 gaaaagctag tcccaacaat acagcccaga ataagacgcc aaggtaattc gcatcaataa
     2641 gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca ctttccaaac
     2701 tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta aaaattgaac
     2761 ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag atcaaaatag
     2821 gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc gtcaagaaaa
     2881 aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc aatattgaaa
     2941 taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata ccaacaacaa
     3001 tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc tttcacaatt
     3061 ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc taaaaattct
     3121 aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca cgttgttttt
     3181 tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg ttttcttatg
     3241 tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa ggagagttat
     3301 ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat attgatttca
     3361 gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta tacattttta
     3421 caaacttccg ttgttattgg tgcaaatttg atcccacttt acatgggtta ccaatatcaa
     3481 aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat attcctaata
     3541 gccacactag tttgcaatat ctttttttac gtacaagctg gcagcttgaa tattcgtgat
     3601 tattggctta tcctaacacc tatcagtcag aattacttta cttatgctgt ttcttgtact
     3661 ttgctttttt gtagtattcc ttatacagtt ggatttttaa ataaattatc gaaaacaaca
     3721 attaaatatc tactattatt tttaacaatt ggattagttg gctgctctac tctatttaat
     3781 aaagacccgt ttgcattaca gaatggacag agtattttat ggatttttta tttattttta
     3841 atgggttatg gtttgaaaga atggaactgg aaagaaaagg tccgctataa gcttcctcaa
     3901 gtatttatca gcttgattgt tttattcggt ttgattattt tgatgacaca aatttcactc
     3961 atcatcagag gaaatacaga tacagcgctg aggttttcta aaccatattc tctcttcgca
     4021 atgtattata ctttctcttc gtttttattt ttagaaatgc tatctgataa attaggtata
     4081 aaaggaagag gaaattttct tgctgtattt ttgattgtca ctcaagttat atgtaatttc
     4141 cctttagttt cttatcgcgt atctacattt atgaaacgtg aatttccaaa ctcagggaag
     4201 gcatggctga taaatatcgg gcagtttttt atttgatatt tattagcagt gtccctccta
     4261 gttatctttt tattttatat acaaaaaatc cgtttcattc atcgtttcat cgaatacttc
     4321 gcttttgatt cgattgaaca attaactcag cgttggaaaa aaagaattca ttggcttttt
     4381 gttcataaaa gttatttttt agttgcagca tttttttact gctttacctt cgtgcagatt
     4441 ttcgttttag aaccaaaaga aggttggaaa gaaactatcc aggttgcctt aaaaatattt
     4501 actcagcgac aagcacccat gatcctgaca atcatcatta ttatgggatt tttcttctta
     4561 ctattgttga tttccaatcg tttttggtac gccttgactg ctaccttgat cattaactta
     4621 ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg aagagcctgt ctttccttca
     4681 gatttaaaaa tgctgaccgg attgtcagaa ctattatcga tggttagccc agtcttatta
     4741 atcgtgggcg gattgatcct tctcctatta ttgattacta gtattattgt acaacgtaga
     4801 ctacaaaaac agtattcctt gaagattcat tggaaacgtc gatttatcag tatcgctgta
     4861 ttattaggat gtttttcggg tgtctttttc attaatcaca aaaattcgcc ttctttcttg
     4921 ctgtttaatt tatttaaagt aaacaagact ttctttaacc agcaaggcgc cgtaaaagat
     4981 aacggaccag tcattcaatt cctaaacaat atcgacatta aaatcatgga agaacctgct
     5041 ggctattcga aagaaaaaat taatggaata atgaaaaagt acaacacaaa ggcagatgaa
     5101 atcaaccaaa caagaagtga ctggttaaac aatcagacga tcgttctcaa tttaagtgaa
     5161 agcttcagtg atcctgcacg tgtccctaat ctgacagtac ctacagaccc tattccttac
     5221 atcaaaagca ttataacaga aggaacagga ggattgatgt tatccgtcgg ttatggtggc
     5281 ggaacagcta acatggaatg ggaaggactg acaggcttga gtatcagtaa cctgtctgct
     5341 tcgttagtta caccttatac ccagctagtg gagcgtcaga aaataagtcc aaatattacc
     5401 gatctattcg atgaaaaaat agccatccat ccttttacag ctgctcttta tcgcagaaaa
     5461 caagtgtttg ataaatttgg ttttgacagg ttctattatt tagacagtcc ggataaatta
     5521 acttatacgg ataaaataga acaaagccca cgcatatcgg atgattcagc atacaaagaa
     5581 accttgaaag ctttacaaag caatacggaa acgtcacaat tcatccaatt atccactatg
     5641 caaaaccata tgccttatga tgattactat agtgaattga actacactgc tgaaggaact
     5701 gctgtcattc ctaatagaag aaaagaatta caaacttata tgcaaggcct tcactatacc
     5761 gatacagcag taaaaaaatt catccaagaa atcgacaaaa tcgataaacc gattaccttt
     5821 gttttttatg gtgaccattt acccgcaatc tatagtggga atgattcgaa aaaatatgga
     5881 ttagaacagc accagacaga ttatttcatt tataacaaca agtatagtcg tgaacaagca
     5941 gctacgttaa ataaagaagt tgttgctcct aataactttg gtgctatggc tcttgagcat
     6001 gcgaatatca aagtaactcc ttactatgcg ttgttgacag aagtatctaa tcatctacct
     6061 gcattaacaa ttgatcctac agaaagcttg tctaatcgtt ttaatggaaa acaggtcttt
     6121 gttacagagg atagtcaaat tatccaagaa aaaaatttaa ctgagaaaca aaaagaaatt
     6181 ctagaagatt atcgtctgat acaatatgat cttgttgcag gtaaccagta cagtgcaaca
     6241 tgggcgacac aaaaaataaa agactaacaa ataaactgag caaggggttg tgataaaaga
     6301 catgtcacaa ccccttgttt atctcattaa tttgcttgtc cataaacgat ttgattaaag
     6361 ccatttttca attcattatt cgctgtctcg ttgaagtaaa gataattatc gggattcccc
     6421 tgattttgtt gtgtcaccaa tacttttggc tgattttgct gaaacccgaa tagatagacg
     6481 tgctttttca gatatggttc tgtctctgca tcggaataga cggctgtgat ttgatagtct
     6541 gcttgacctg tcccactttc tgaccattct gcagaaacag gcactccacc aatagccatc
     6601 ttccactccc cgttcaaaat agcttgaggg actttaagac catataagtc cacactcatt
     6661 tgattcgtat aacttttgta ttgttgtccc attgtttggc cccatttggt cataaatgcc
     6721 tctaattgtg cggatttgtc tgcattccac agtgtttgtt caacttgttc gctgctttct
     6781 gaagaggaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt tgtttcagtc
     6841 gttttggaag actctgacga agtagaggta tctgctgttt ctgtacttgc tgcagttgac
     6901 ttgctctctg tgctagttgt tgtagtagtt gcaactgttg ctttcgctgt ttcttttgtt
     6961 gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc tagaccaatt
     7021 gttactaaaa tccttttttt caattaaaaa actcctttcg attactccca ct
//
LOCUS       DAZKNM010000133         6777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000133 DAZKNM010000000
VERSION     DAZKNM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.135"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..732)
                     /gene="pgsA"
                     /locus_tag="ACG8M9_002213"
     CDS             complement(154..732)
                     /gene="pgsA"
                     /locus_tag="ACG8M9_002213"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0378934.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(941..1864)
                     /locus_tag="ACG8M9_002214"
     CDS             complement(941..1864)
                     /locus_tag="ACG8M9_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0378935.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(2603..3889)
                     /locus_tag="ACG8M9_002215"
     CDS             complement(2603..3889)
                     /locus_tag="ACG8M9_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0378936.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3882..5150)
                     /locus_tag="ACG8M9_002216"
     CDS             complement(3882..5150)
                     /locus_tag="ACG8M9_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0378937.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5402..5863
                     /gene="mscL"
                     /locus_tag="ACG8M9_002217"
     CDS             5402..5863
                     /gene="mscL"
                     /locus_tag="ACG8M9_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0378938.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5922..6368)
                     /locus_tag="ACG8M9_002218"
     CDS             complement(5922..6368)
                     /locus_tag="ACG8M9_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378939.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(6461..6706)
                     /locus_tag="ACG8M9_002219"
     CDS             complement(6461..6706)
                     /locus_tag="ACG8M9_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0378940.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         1918 a         1440 c         1194 g         2225 t
ORIGIN      
        1 tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac
       61 aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga
      121 cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct
      181 gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat
      241 gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat
      301 aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc
      361 tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat
      421 cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac
      481 tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc
      541 acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc
      601 tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg
      661 taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt
      721 tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt
      781 ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt
      841 gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt
      901 atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag
      961 taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt acggattctc
     1021 cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg
     1081 tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg
     1141 cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca agggttacag
     1201 gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta
     1261 ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg
     1321 tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag
     1381 aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga
     1441 caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc
     1501 gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct
     1561 gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat
     1621 ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt
     1681 taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt
     1741 ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt
     1801 tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa
     1861 gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca
     1921 gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata
     1981 tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc
     2041 aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa
     2101 aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg
     2161 ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
     2221 caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca
     2281 gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata
     2341 agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     2401 gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt
     2461 tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga
     2521 ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga
     2581 cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
     2641 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
     2701 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
     2761 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
     2821 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
     2881 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
     2941 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
     3001 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
     3061 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
     3121 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
     3181 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
     3241 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
     3301 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
     3361 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
     3421 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
     3481 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
     3541 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
     3601 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
     3661 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
     3721 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
     3781 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
     3841 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
     3901 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
     3961 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
     4021 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
     4081 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
     4141 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
     4201 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
     4261 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
     4321 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
     4381 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
     4441 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
     4501 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
     4561 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
     4621 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
     4681 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
     4741 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
     4801 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
     4861 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
     4921 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
     4981 tgatccgaat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
     5041 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
     5101 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
     5161 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
     5221 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
     5281 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
     5341 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
     5401 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
     5461 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
     5521 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
     5581 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
     5641 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
     5701 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
     5761 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
     5821 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
     5881 agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc
     5941 tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat
     6001 ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct
     6061 ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct
     6121 ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat
     6181 actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttct
     6241 ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt
     6301 cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga
     6361 tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa
     6421 aaagcaaacg aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa
     6481 attcgatctc aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag
     6541 aattttcttc aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc
     6601 cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga
     6661 tacgaccttc taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta
     6721 ctatcaacta taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaagaa
//
LOCUS       DAZKNM010000134         6757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000134 DAZKNM010000000
VERSION     DAZKNM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.136"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..1633
                     /locus_tag="ACG8M9_002220"
     CDS             80..1633
                     /locus_tag="ACG8M9_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0378941.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2014..3798)
                     /locus_tag="ACG8M9_002221"
     CDS             complement(2014..3798)
                     /locus_tag="ACG8M9_002221"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378942.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(3813..5534)
                     /locus_tag="ACG8M9_002222"
     CDS             complement(3813..5534)
                     /locus_tag="ACG8M9_002222"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0378943.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5634..6395
                     /locus_tag="ACG8M9_002223"
     CDS             5634..6395
                     /locus_tag="ACG8M9_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0378944.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         2141 a         1203 c         1322 g         2091 t
ORIGIN      
        1 ataaattttt tattagtggg actagtgatt gtatggattt tgacgcataa tgaatgaagt
       61 atgagaaaag gagagacaaa tggatcaaga actatttatt atcgaagtat tatcagctaa
      121 acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga ataatgttca agcttttaag
      181 cttagtattt ttctctggtt tgcttttttt cggagctgta tcgttcctat atcgccatat
      241 gagtgcagta gtccgttcag ctgaagggat gcaaaaatta aataatttgt atcacttttc
      301 gtggggagtt ggcggaataa caattgttgt gcttattgtt tttcttctac ttatttggct
      361 ccgttaccat caattgtcgc gtcatttcga tcaatggctt agtcaaagag cacaacagtt
      421 tttacataat aaaccaactt ctcaagatga tgactatctt tattttgaga agacttcgac
      481 gagtcaagct tttcggatac aaaaaagtga ttgccagtta ttgctgagat cacttgatga
      541 tttacctatc tacttaggcg aaagcaaatc aaaattcgga atgaacacat tccatatttt
      601 tttactagaa aacgaaccca gatgggaaaa aagccagtct tcgcctggtg gatacctgac
      661 aactaagatg agtacattag tcactggttt tttcctcatt cttttagctg gaacaatcta
      721 ttatgggaga tacactcagc caaaaatgtc ttctcatata aatgctgctt ctcacatgac
      781 aatgaactca actaacgata cgcatataca aaagacaaat gaagggttgt tagctgatcc
      841 taagcaaaag gtaaatttga caacacaaaa gataaatgat ctcaaacttg atacagagac
      901 gcactctctc tatatgacaa cagatagtgg tgctaattgg gtatttgtgc cgattgattc
      961 gcaatggttg cgttttggcg aatacaccct gacaacgggg ctgattcctg aaggttattg
     1021 gatggacaac acatatgata tttccgccga tttctcatgg tttatctatt cttctgggga
     1081 tgatgtacgt ctgctgactt caagagataa tggtaaaaca tggcaaacgt ctattatcac
     1141 ccaatcagtt cagccgatac gttatcgaaa agcggtattt tatggcaacg atggtctatt
     1201 tatctgtaca tcagccagtg ggatgtctgc agaaactttg tacatttaca ctactatgga
     1261 tggcggaatc acttggataa aaagtggaag tacaatgatc aatcagccag tacaaaatgt
     1321 cagcttcgtt acaacttctt taggttttgt ttcgacacgt gaaagtttgt tttatacaaa
     1381 caacggagga cgctcattca aagaatcagt tctgtcgatt ccggaaaact atcagacagg
     1441 ggggatagat ctttttcaat caccaaatga agttactcaa ataagcgcga atacgttaga
     1501 gacaaaattt tatttgttga aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct
     1561 cttccgttca actgataacg gggaaacatg gcaattcgtt cagcaactat cgcaagtaga
     1621 acaaggagat taaagaagag agattgtgag cctgacgttc agcaccgagt cacaagaaac
     1681 aaattcaaaa aacagcttct catatttgtt gaatagtgta actcgtgatt cggagttgac
     1741 aagagcaaca agaagcgatt ttgaagtata gcttctcgta ttttacaaga tcgtgtctta
     1801 ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa aagggattgt gacaagtctt
     1861 ttgtcactac ttaccgaacg aaactgaagg agctatccaa aaagtatcat tagtattatt
     1921 ctttgtgttt ctagtggcac cactagtaca tttaaaaaag ctatcgatca tggactaatc
     1981 atacatgatc gatagctttt tagataatgt ttgttacgct atctgaaatt gactattata
     2041 aagactggca taaagcgttg gtgattggag tagttcagaa tgtgttcctt tttcgacgat
     2101 atcgccattc ttcattacaa ggatcatgtc tgcattttca atcgttgaca aacggtgtgc
     2161 aatcacaaaa cttgtcctat cttttgtcac agcttccatt gcttgctgaa tcaatgcttc
     2221 tgttcgtgta tctacgctag aagttgcttc atctaaaata acgacaggag gattcgctag
     2281 aatgactcga gcaattgtca ataattgttg ctgtccttga gataacgtgc cgttttcgct
     2341 agaaatgatc gtatcatatc cgtctggcag tgttcgaata aagtgatcac attgtgcgat
     2401 ttcagccgct tcgataattt gttctcttgt ggcatccatt ttcccgtagg cgatattatc
     2461 agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag
     2521 ttctccacgt gaaagcttgg taatatcaag accgtcgaat tcgatctttc ctttattcag
     2581 ttcataaaaa cgcatcagca gattgactaa agtcgttttt cctgcaccag ttggaccaac
     2641 gatcgctacc gtttgttttg gctttactga aaaatcgact tgattcatca atattttatc
     2701 tgatgtgtaa ccaaactgta catttttgaa agctattttt cctttcggtt gttctaaaag
     2761 tcttgcatct ggaaggtctg gaatttcatc tgtttcatct aatatctcaa atatcctatc
     2821 gattgctgcc attgcggatt ggatcgagtt gatcacataa gaagcggtag aaattggttc
     2881 agaaatctga ttgatgtact gaagataagc ttgtaagaag ccgatcgtca caccacctga
     2941 tagaacaagc atagcaccga ataccgcact tatgatgaag gcgagttgat taatgaaacg
     3001 aatggccgga tagatagcac agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt
     3061 tttatttact tcgtgtatag cagcttctgc atgagcttgc tgattgaatg ttttgaccac
     3121 tagattacca gctaagattt cttccattcg attatttaaa acacccagtt catactgatt
     3181 ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt gtaacaattg aactgccaac
     3241 gatcagcaat aagacgagca aagtaagctt gctttcgata taaaacagca tcagaccagc
     3301 agcagctatt gttacgacag aagtaaaaaa ttgattgata cctgtcaata acacttgaga
     3361 aagttgattg agaccggtag ttgtccgact aatgatgtca cctacttgat gattgtcaaa
     3421 aaacgacatc ggtaatgttt tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat
     3481 cttttcgctt aacgctgcca ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac
     3541 tgaacagccg atcagcaata agacagggaa caacaatgct tgtttgatat cactgaaaga
     3601 aagctgtcca atacctgttt ttcttatttc tgataagaga tcatcgatcc cgattcccat
     3661 gatcaagggc atggctacga ctaataggtt cccgaataaa ctgcatagaa acaatccgta
     3721 aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa
     3781 cgtttttttc ttttccatcg tatcccttct ttctatattc cttgtgattt tgcaaactcc
     3841 tgataagttt ggcaattaga cagtagttct tcatgagtac cttgtccaac gatttttcct
     3901 tcatctaaaa caatgatgtt atctgcttgt tggattgtac tgaggcgttg agcaacaatc
     3961 acgagagttt tctccgacat gtgtttatga agggctgctc ttaatgctgc atctgtttta
     4021 tagtctaaag cggagaaact atcatcgaat acatagatct ctgctggttt gatcaacgct
     4081 cgagcaatac atatccgctg tttctggccc cctgaataat tagaaccgcc ttgagcaaca
     4141 aagctgtcca atccgtcagg aagggtgtcc actagttctt ctaattgagc gatcttgatc
     4201 gctttagaca actcttctgt tgaggcatat tcatttccca tctgcaagtt gcttaaaatt
     4261 gtcccgctga ataagaaagc cttctgaggg acataactga tgtgggagcg gatctgttct
     4321 tgagtcattt gagtaagagg aataccacca agggttactt ttcctatcgt agggtcacta
     4381 agtctcaaga gaagtttcgc caaactactt tttccagatc cggtcccgcc aacgatcgct
     4441 gttgtttttc ctttaggtat tgtgaaatca atatcttcta gaactggatc agagatttca
     4501 gtatatttaa atgtgacatg ttcaaaagca gctgcatttg tctgatcaaa ggttggttca
     4561 gctactgttt tgatcgtctc ttcctcaatt tcgatctctg cttgcagtat ttcttctatc
     4621 cgtcgtaatg aggcaaacga tttagggata atgaccaata ccatagctgc gatcatcaag
     4681 taagcaagtg tcagcataga gtattcgact acagcagtca cgacaccgat ttgtaatgct
     4741 ccttctgcta caagatttcc gccaaaccac aaaatagaag cataaacgat tcccatgagc
     4801 aaaaaagcaa ttggagtgac ccaagcaaat attcggttca ctttgatcat ttgattagca
     4861 tagtcagcaa agctcttatt cgttcgcttt tcttcatatg attgattatc aaaggcacgg
     4921 atcatattaa tcccagtaaa gaattctctc aatgtgatgg tgatccgatc catttttggc
     4981 tggatactca aagataaagg tgcccctcgt ttcatcagtc gataaacaat ccatacataa
     5041 aaaacaacag agatcaatgt aacccatgtt agtaatagag aataacgaaa catcatgaga
     5101 atggtgaaaa cgcaaataac cggtgctggt aagatcattt ggcaaaataa aacaatcatt
     5161 tgctggacat tatcgacatc attcgtcatt cttgtcaaaa gagatccaga actaaaacga
     5221 ctgatatcct tcattgacag ctgttggaat ttacggaaat atttctcccg cgtttggaat
     5281 ccaaacctag cggaagccaa tgctgaaaag taactagagg caactgcagc gattgttcct
     5341 agaaacgcta caagcagcat ttcaactcca atttttttta taactgaggc ttcgccactt
     5401 gctatacctg tgtcgatcag ctttgctact aattttggta cacctaatgt accggcgatt
     5461 tctaaacaga tcatcacaaa ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt
     5521 tttaagatag acatattatt cctccttttt tcctgatact tttcgattct ataccttcaa
     5581 gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa
     5641 aaatgtatac gattggccaa gtatcggaac tatttgattt gccaaaatca acgatccgct
     5701 attgggatga acaaggatta atccgttcaa gtagacaaga agagaatgat tatcgattgt
     5761 ttgatatcga tgatattttt atgctttatg atatcgtttt ttatagaaaa ttagatatac
     5821 cgatcaaaca gatgaagaat ttatatggaa aaacattgac ggagttatat gagacacttg
     5881 atgaaacaga aagaagaatc cataaagaac tggtggtgat gaaacaaaaa caaaaagaga
     5941 taagggagca aaaaaaacag ttgaaattga tgatcgatac aaacgaggaa gaatttcctg
     6001 tagaagagat cccttttgac tgtatgatct caacggagtt tgaagatatt gtggaaatca
     6061 aaaaatttct acctaactat tcaagctttg ggatgatgag tatgccagct tccagttcac
     6121 agacgatgta tggatttttt atcgacccta gtgaagtaca tttatttact caagacgtga
     6181 tttgggagaa aaaagatacg gctgtttatc gtcgattctt attgaaatcc gagatgaacc
     6241 atgcggaacg taataatatt ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa
     6301 caggagaagt gatcggccag tatctattga cgaatacgga tgaaaacaat atcagaactg
     6361 aatattatca tgcatggata gaaatgaaaa aataggaaat atgtgtttaa gataactcga
     6421 aaatgatggt tgttttagtt ataaagaaaa attttctggt tcgattacgg gtttaatgtg
     6481 ggggattatt agtcgtggat agatagaatt ttagtttttt agaggaaata tgtctttaaa
     6541 aatggaatgg ttgcaattct tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat
     6601 ggaaaaataa ttgtattggg tgttatgtat aattcatggt gaactgagac taatgagaag
     6661 aaaaattcat attgttatga gtgagattgt atcagatgag aatgataaat taaataggtg
     6721 cataaaagca ttacacttaa aatacattgt tgcttac
//
LOCUS       DAZKNM010000135         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000135 DAZKNM010000000
VERSION     DAZKNM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.137"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8M9_002224"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8M9_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8M9_002225"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8M9_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0378945.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8M9_002226"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8M9_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8M9_002227"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8M9_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0378946.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8M9_002228"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8M9_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0378947.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8M9_002229"
     CDS             3582..4271
                     /locus_tag="ACG8M9_002229"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0378948.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8M9_002230"
     CDS             4285..5787
                     /locus_tag="ACG8M9_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0378949.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8M9_002231"
     CDS             5800..6276
                     /locus_tag="ACG8M9_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0378950.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8M9_002232"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8M9_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKNM010000136         6569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000136 DAZKNM010000000
VERSION     DAZKNM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.138"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..473
                     /locus_tag="ACG8M9_002233"
     CDS             93..473
                     /locus_tag="ACG8M9_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0378951.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            569..1078
                     /locus_tag="ACG8M9_002234"
     CDS             569..1078
                     /locus_tag="ACG8M9_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378952.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            1155..1334
                     /locus_tag="ACG8M9_002235"
     CDS             1155..1334
                     /locus_tag="ACG8M9_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378953.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            1389..1952
                     /locus_tag="ACG8M9_002236"
     CDS             1389..1952
                     /locus_tag="ACG8M9_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0378954.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(1999..2514)
                     /locus_tag="ACG8M9_002237"
     CDS             complement(1999..2514)
                     /locus_tag="ACG8M9_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0378955.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(2540..3409)
                     /locus_tag="ACG8M9_002238"
     CDS             complement(2540..3409)
                     /locus_tag="ACG8M9_002238"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0378956.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            3535..4215
                     /locus_tag="ACG8M9_002239"
     CDS             3535..4215
                     /locus_tag="ACG8M9_002239"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0378957.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4368..5348
                     /gene="pta"
                     /locus_tag="ACG8M9_002240"
     CDS             4368..5348
                     /gene="pta"
                     /locus_tag="ACG8M9_002240"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0378958.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            5471..5944
                     /gene="tsaE"
                     /locus_tag="ACG8M9_002241"
     CDS             5471..5944
                     /gene="tsaE"
                     /locus_tag="ACG8M9_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0378959.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            5937..6461
                     /locus_tag="ACG8M9_002242"
     CDS             5937..6461
                     /locus_tag="ACG8M9_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0378960.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
BASE COUNT         2180 a         1165 c         1389 g         1835 t
ORIGIN      
        1 gaaaaacagc tatttttttc aatctgacac atttacaatg gaactggaga ttaatataga
       61 tccgagctta aacgaagaat aggagtgctc atatgaacct tacaacgatc catcatatag
      121 ccatcattgt ttcagattat cagaaatcac gtgattttta tgttaataaa ttaggatttg
      181 aagtaatcag agaaaattac cgcaaagaac gagatgacta taaattagat ctgaaactag
      241 gaagtgctga attggagatt tttggaattg ctaatgcgcc acaacgacca aactatccag
      301 aagcttgtgg cttgcgtcat cttgctttca aagtagacga tattgaagaa acaattgcag
      361 aattgcagtc cttagggatc gaatcagaac cgattagaga agataccttt acaggaaaaa
      421 aaatgacgtt tttctttgat ccagacggat tgccattaga actgcacgaa taaagataac
      481 aaaattgtca cttttttttc gtttcattcg atttttcatt cgttcatttc ttgatattct
      541 ttaaaaaaaa gaaacaggag aatgaagaat gaaaaaaatg atgtttggtg ttgctgccct
      601 catcgtaata gggattaccg gtatgacggg actgctcaat gccgcttttc acatggatga
      661 tattagtgac caggaatatc ggatctcatt gaaaaaagca gcagcaatcg taaaaaacga
      721 aacaaaagaa cataacatat ctgctattca ttttgcagtt gaaaaagatt tggagaatcc
      781 tacgaatgca acatatgaat accagttttt ttgtgaagga caagtcatag cagtcaatcc
      841 atccactggg aaaacaaccg tacgaaatga cacggaaaag gccgcagaac atctttttga
      901 tatagaaaaa acggaagagt tcaaacaacc gcaagcagcg atgaaggaag cagtcagcta
      961 ctacgggaaa aaagcaaaag tgaaagagtg gacacttcta gtcaaagatg aacgtcctta
     1021 ttataaagtc cgattaatag atggagaaca ggaccgtcag gtatggatat ctgcatagaa
     1081 gaaggatatt gcttctcttt tttacaatat aagagtaagc taagagagta aagatgaaag
     1141 gaagttgaga taacatggaa aaaatgaaat gccctaattg cggaaaaaaa tttgcatacg
     1201 aagaagtcaa taatgtagtc gaacataatg ataaagaaat gccagtcgtc tgcccttatt
     1261 gccgaactga agcggcaaga atcgttactc atggttattt cattactgaa aagattgaag
     1321 attttttaaa ataacgattt tgcgatttct ttttctgcat gttagaatgg agaaaaggag
     1381 aggaaactat gattcgtttt gcgagaaaag aagatgctcc agaaatcgcc ccattgatct
     1441 tagtgattct aaaagatatg gaactgccat ttttgaagca gacaggggaa caaaagactt
     1501 tagagatttt agaagaagca atcactgatc caaattatcg ttacagttat tcacgaggta
     1561 tcgtagaaga aatagacggt aaagttgcag gagttgctta cggttataca gatgaagaag
     1621 aatcaatcat cgatcagcca ctggtaccta tcttagagaa atatcagcta gatgcgtcta
     1681 cacgtttgtt cattgataaa gaaacgtttc ccaacgaatg gtatttggac tctatcagtg
     1741 tatcagaaga ttttagagga caaggcatcg gttcgcgatt attagaagca ttacctaaat
     1801 tggcgaagaa agctaatcgc tccgtcatcg gtctaagtgt ggatgaaaaa aatccaaagg
     1861 ctaaaaaatt atacgaacgc catgacttca aagttgtcgg tcagcggatg atcagtggtc
     1921 atctttatga tcatatgcaa aagaatatct aaaaagatgt acctgaaatg cgtatttccg
     1981 catttcaggt acatcttttt aacttaagtt aagtcggtaa gaaacaggct tatcgcgact
     2041 tttgtcttta cggtagagcc agtaatctaa aaccgcagca atacaaatac aaactggagc
     2101 atcttcatca tttgagacat ccaatacata gtaatctcct gaaaaaagtg ttgctttatc
     2161 cattttcatg atttgctgat tgaaatgatg gatttgatat tgatgattat agatatcacc
     2221 ttggactgtc cagtgaagtc tacgaatata ataaaagtca ccgggccaat tgaatatttt
     2281 ctgcatggtt cccacctttt cgaactcttt atacagctca aaacgcgtac cgaatgtcag
     2341 gcttatctgc ttgatgtgag caacgagatc gccattcatg gcataaagag acaatgcatc
     2401 gccctttgtt ccccagcgac caaccatcaa aaacagggaa tgtccttttt cgtttttgac
     2461 aattgtccga gttgcacgtc caagctgctt ttcctgaatg aaaaattctg acatctatat
     2521 ccctcttttc tagataaaac tacaagtctt ctgcaatcgc gcgcaatatg gcttttccta
     2581 cgccagattc aaggtttgtg tccgttgtat atttggcata atcttttact tcgatttctg
     2641 catttcccat tgcgaaactg actcctgcta cttgcagcat gctgacatca ttgaagttat
     2701 cgccaatcgt catgacttct tctaaagaaa tcccacgctc gtgagcgaca tgggcaacag
     2761 caattccttt ttgagcgttt ttatggttca cttcgatatt gttttgacca gaggaagtaa
     2821 cggctagatc gcctaaaaga gaaatctgct ttccaactgg acctaataca gctggccctt
     2881 ctgtatgaaa acaaataatt tttaaaactt ccagattctt ttcttccaaa atagcacgaa
     2941 tactgtccac gtatgtgatg tgcaataact ccagcttagc tgaggccatg gcaatcgcca
     3001 ttttatacgt aaggtgaggt aaatgggtag ccaccatgga agcaaaactt tcaattcgtt
     3061 tcgcttgatt ttcagaataa agaccatcgt tggttgctac ttcatagtaa ataccgttag
     3121 catctagaat atctaaaaca gagattgctt gattatttgg aatcgacacg gtaaacaatg
     3181 aatttccttc tttatcaaat acctgtgccc cgttcaaagt gatcatcgca caatcgattc
     3241 ctacctcatc taaagctgcg cgagcttctt gatagtttcg accagtagcg accatgaact
     3301 caacaccggc atcatttgca aaacgtatcg cctcggcatt ttcaggagaa acactcatat
     3361 gggcatctaa taatgtgccg tccatatctg atgcaattaa tttgatcata atgattatcg
     3421 ttcctttctg cttcttgttc ctagtttacc atattaatca agtgctgagg gtgaagattg
     3481 tcccttgtga ttgaagaaaa tgtgagttat tctttttatt aggaggtgga gtttttgaaa
     3541 gaaatcattc ataacagttg gcaagaagtt ttgtcctcag aattttcaaa agattattat
     3601 ctgcatctaa gagaattttt aaaaaaagaa tatgcgtccc aaaagatcca tccagatatg
     3661 tatcatatct atgaagcatt ggaattgact ccttatgaag aagttaaagt ggtgatttta
     3721 ggacaagatc cttatcatgg agaaaatcaa gctcatggct tatctttttc tgtgcaacct
     3781 ggcgtaaaga tcccgccatc gttaagaaat atctataaag agctttatga tgatcttgga
     3841 atcgcacctg ttcaacatgg aaatctagtg tcatgggcta aacagggagt ccttttactc
     3901 aacaccgttc ttaccgttcg ggaagggcaa gcgtattctc atcgcgggaa aggttgggaa
     3961 agattaacag acacgatcat tgaaaaatta aatgaacggg aaaaaccaat cgtgtttatc
     4021 ttgtggggaa aaccggcaca agaaaaaatc aaaatgatcg ataagagtcg ccatatcatc
     4081 ataacatctc cacatcccag tccgttatct gcaagtcgtg gcttttttgg ttccaaaccc
     4141 ttctcgaaaa cgaatgatgc gctccttgcg ttaggagaag aaccgattga ttggcaactg
     4201 cctgaaacag tgtaataaaa cagcgctttc ctgttatagt gcagttcgct ttgtatgtga
     4261 aaaacttaat ttttccagca tttttgactg atacagataa aataaactgt tctttttttc
     4321 tggaatagtg tatgataata catataaaaa cgtctggagg tcatcttgtg gaattgtttg
     4381 atagtttaag atttaaaatt gttcgccgtg ggatcaaaat cgttttcccg gaagctactg
     4441 atgctcgtat tttaggcgca gctgcccggt tgaaagcaga agaacttgtg gaacccattt
     4501 tagtagggaa cgaagaagaa ataagaaatg cagcgcatgc cagaggaatc aaaacatcta
     4561 gctttacgat catcagccct gataattacg ataagtggga tgaaatggtg gatacttttg
     4621 ttgaacgtag aaatggaaag gcatccaaag aacaagcaga aaagatttta aaagatgtga
     4681 attactttgg tacgatgctt acgtatatgg ggattgcaga cggaatggtc tctggagcga
     4741 tccattcgac tggcgacact gttcgcccag cacttcagat catcaaaaca aagcctggca
     4801 tcagtcggac aagcggagcg atgatcatgg tacgtggaag agatcaagaa aaatatgtct
     4861 tttcggattg tgcgatcaac gtgaatccaa ctgcgcaaga attggctgaa atcgccgtag
     4921 attctgcaaa aacagcagaa ttgttcgata tcgaaccaaa agtagcaatg atgagcttct
     4981 cgacaaaagg ctcagcgaaa tcgccagaag tagacaaagt agttgaagca actaaaatcg
     5041 ctaaaaaact agcgccacaa tacatgatcg acggcgaatt gcaatttgat gcttcttatg
     5101 ttccttcagt tgcacaactg aaagcgccag attcagaagt agctggtcaa gcaacagtct
     5161 ttgtatttcc agaattgcag tcaggaaata tcggctataa aattgctcag cgtttcggta
     5221 actttgaagc aatcggacca attctacaag gcttgaacaa accggtttct gatttgtctc
     5281 gtggagcaaa tgaagaagat gtatataaat tatcgattat cacagcagca caaacattga
     5341 ttgattaaca aatacacgcg aactagccaa aaacagcagc tgctgttctt gactagttca
     5401 ttttttcttt aaaaaaactt cattacgcga tatactggca aaaagaaaga agagcgggag
     5461 aagatgaaaa atgatcgaac tatcaggatt aacaatgaca gaacaattcg cagaggcaat
     5521 tggtcattcc gcaatgccag gtgacaatct gatattgact ggtgatctag gtgcgggtaa
     5581 aacaacgttg acgaaaggaa ttgcccaagg attagggata acgcaaatga tcaaaagtcc
     5641 gacatacacg attattagag aatatagtca aggtcggatt ccgttgtatc atatggatat
     5701 ttatcgggta gcagctagcg gagcagattt agggttagat gaatattttg aaggtgatgg
     5761 gctttccgtt gttgaatggg gaaatctatt agaagaagca ctgccggagg attatttgga
     5821 actgatttta gaaaaaagtg atacagattt agaaaagcgg tatgtgaaac taatctcata
     5881 cggggagcaa agtgaaacct tcaaacagcg aattctagaa aaatgggctg aaaatcatga
     5941 gtgaagaagt tgggatcatc ttgcgggaag cagtgccagg agatgcaaaa gacattcttt
     6001 tgatgatggg acaagttaat aaggagacag aatttttagt tttggatgaa gcagaactcc
     6061 tgttaccacc agaaacctta gaagaagagt tggattatat atacgaaagc aataataatt
     6121 tattgctgct tgcaatctat gaagggacga ttattggaac agcatctgtc aaggcagaca
     6181 gtcaatttcg tttgtctcac gtgggagaag tcggtatcag tattctgcag gaatattggg
     6241 gaatgggact aggcactctt atgctagaag aaatcatcag ttgggcgaag gaaatgggtg
     6301 tactttttcg attggagctt gacgttcaag ttcgtaatga aagagccgta cacctttacc
     6361 gaaaaatggg cttccaaatc gaagcggtca tgcctagagg ggcaagaaca gatctaggtg
     6421 aatttttaga tgtatataaa atgagttatt tgattgaata gaagaaatag gaaaagagtg
     6481 agaaactgtc atccttttga gaaggcagaa aaacacacga agggactgtg acaaaagtcg
     6541 tgtcgcagtc catttttttt gaataaacg
//
LOCUS       DAZKNM010000137         6554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000137 DAZKNM010000000
VERSION     DAZKNM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.139"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..797
                     /locus_tag="ACG8M9_002243"
     CDS             111..797
                     /locus_tag="ACG8M9_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0378961.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            799..1743
                     /locus_tag="ACG8M9_002244"
     CDS             799..1743
                     /locus_tag="ACG8M9_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0378962.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1942..2586
                     /gene="pcp"
                     /locus_tag="ACG8M9_002245"
     CDS             1942..2586
                     /gene="pcp"
                     /locus_tag="ACG8M9_002245"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0378963.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2665..3942
                     /locus_tag="ACG8M9_002246"
     CDS             2665..3942
                     /locus_tag="ACG8M9_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378964.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4200..5417
                     /locus_tag="ACG8M9_002247"
     CDS             4200..5417
                     /locus_tag="ACG8M9_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0378965.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(5738..6487)
                     /gene="pnuC"
                     /locus_tag="ACG8M9_002248"
     CDS             complement(5738..6487)
                     /gene="pnuC"
                     /locus_tag="ACG8M9_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0378966.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2116 a         1215 c         1363 g         1860 t
ORIGIN      
        1 ataacgaaaa atatttaaaa atagtgtcta tttcgtgaga aaacaactat attttttatg
       61 tgttttgctt tataattaaa aaaatattgt cattttatgg aggaaaatat atggaaaatt
      121 atcttagcct tttaggcgtc ttgattgtta ttctcgggtt tgcatttaaa ttagactcga
      181 tattgatcgt aatggttgct ttagttgtta cagctttgac tggcggtctt ggcattcaag
      241 gaatgttaga acttttaggt acaagttttg taaacaatcg cgggatggcg atttttatca
      301 tcattatgtt ggcaacagga acgcttgaaa gaaatggtct gaaagaatca gcggcttcct
      361 tgatcaagcg attcaagaaa gtatctgctg gtatgattgt agatatttat gggatctttc
      421 gaatgatctt tgcggctttt aacgtgagtt ttggtggagt tgctggattt gttcgtccaa
      481 tcattttacc gatggcattg gggaccatcg aatcaaaagg gttagaagtc catcctaagc
      541 atgaagaaga actaaaaggt atggcttcag ctatggaaaa tgtctgctgg ttctttggcc
      601 aagtactatt catcggagga gcaggggcgt tgctagtcca atcaacactg aaagatctag
      661 gatatgacgt aacattggga cagcttgctt tagtggaagt tccagtggct atcacggctt
      721 tagttgtggc aagtgtctac ttctatctta aagaaaagaa attagcgaag aaatattatc
      781 caggaaaggg gcaataagat ggactttttt acaaatcctg atattttatt aggcgataaa
      841 ttactggaga tcctttatat cgtgatggga ctcgtattga tttacactgg agtaagaaat
      901 ttaacagata aaaccaatcc tcatcgttat gggtcagcct ttttctggac ggtcttaggg
      961 gtagttattg caggaggaag atggcttccg gcaatcgtga acggtggctt gatctttgcg
     1021 atgaccatac cagcaattgc taagaaagtc agcaaaggag aaagccggct tccatcaaaa
     1081 gaatatatgg aaaaaatgtc agacaaacta ggaatgaaaa tttttattcc agctttgagt
     1141 atcggtgtct ttgcgatctt gtttgctcta tttaccaatt taggtgcttt agtcggcgtg
     1201 ggcgtcggtg tttttgcagc catgctaatc ttgatgttct tttctcgaga taatcgacca
     1261 agtacatttt tagatgatgc agcagatatg ctgggcacag tagggccgtt aagcatgttg
     1321 ccaatgcttt tagcttctct aggtgcggta tttacaagcg ctggagttgg gacagtgatt
     1381 tccaatgcag taggtacgat cgttccagaa ggaaatgtga acatcggcat catcatttac
     1441 gctcttggca tggtgatatt tacggtaatc atggggaatg catttgcggc catcaccgtg
     1501 atgacggttg ggatcggtgc accattcgta tttagtcatg gagcgaatcc agcgttaata
     1561 ggaatggttg cattgacttg cggtttttgt gggaccctgc tgacaccgat ggctgctaac
     1621 ttcaatatcg tgcctgtagc gatgctggag atgaaagaca aatacggagt gatcaaaaac
     1681 cagttgttta ttgcgttatt tatgctagtt ttccaaattg cttatatgat tctatttaaa
     1741 taatagaaga gaggggttgt gacagaataa ttgtcgcaac ctcttctaac attgtagtgg
     1801 aacactattt ttttataaga atatattaag tttgatctgt tatgttattt aaactattgt
     1861 ttcctttatt tacgctgttc aaggaaaatg gtatgataaa agataaccat atgttaaatc
     1921 aatcggacgg aggaacaaca gatgaaagta ctagtaacag gttttgatcc atttggtggg
     1981 gacaaagtga atccagccta tgaagcagta aaaaaaatgc cagatgaaat cagcggtgct
     2041 gaaatcatca aagtggaagt accgaccgta ttcgaaaaaa gcagtcaagt agtcaaagaa
     2101 gccatccaac agcaccaacc agatgtcgtg atctgtgtcg gacaagcagg tgggcggtca
     2161 gcagtctcgt tcgaacgtgt cgcaatcaat ctagcagaag caagaatccc tgataatgaa
     2221 ggaaatcagc cattcgatac ggctttagaa gaaaacggtc cagccgctta ctttacctca
     2281 ttgccaatca aagcaatgac aaaaaatgta caagatcatg gtctaccagc gtacatctct
     2341 tatacagcag gaacatttgt ctgcaatgat atcatgtatc gtttgcttca tttgatcgaa
     2401 acacaattcc cggcaattcg aggtgggttc atccacgtgc cattctcacc cgatcaagtg
     2461 atcgatcgtc cagtgggtac gccttctatg tctttagaag acattgcttc ttctttgact
     2521 tatgcagttg aagcagcagt agaaaataaa gaagatattt ccggaaatgc cggaacaact
     2581 cactaattta gcaagaaaca ctggctggtt gctaacgtta tgttagtagc cagctttttg
     2641 taattagaaa ggaaagaaac agccgtggaa gaatggcaat ccgtctttga agaatggttt
     2701 ccaaaagaaa tcagcaaaag ctatcccatc aaaatttcta aacaatacac aagcagtcaa
     2761 cgttgggaga tctatgcaaa attaacgaaa aaacagcggg aactagtcga taagcaccgc
     2821 cgatatctaa tcagctctcg ttttatggaa gaacattatt tagctgcaac agattgggtg
     2881 ttttctgact tcaagatcaa tccgtttttt cgaacaaaac gaagccagca aaaattgtat
     2941 tgtgagtgtg gtagagaatt aaaagtgcaa tacatcgtta aatcgccaaa aaccggaaag
     3001 atcctaaaac taggcatcaa ccactttgcc gatcacctgc atgtctctcc gacagtggca
     3061 gcttcgatcc atcagggaat gacaaaagtt gatctggcct tagacgaatt attatggctt
     3121 aagcagaaga atatcgattt tcctgaagga ctatggcaaa aatactgttt tgtcttgtac
     3181 caaaatcgtc ggatgaaaca gccatattta ccggatataa aattagctca gcggctcgca
     3241 gaatttcgcc aagtagaaat gccgatttac atagcggatt atcaagcatt ggaaaatgaa
     3301 atcaagaaaa taagtgaaca catcaatggg cagtcaaaaa agcgacagat aaaaaaagaa
     3361 ctatttgacg actttgcaga agaattagta aaggatgtag aagaattctt gatcaactat
     3421 cgcgcatttt tacgtaaaga ttggcaatcc atcgtatatg aagaagtgcc agtacatcct
     3481 aatgcttatt ttgaaacatt tatttccgtg ttgagaaaga caaaacgtca acgaacacca
     3541 gaagtcactg ctcagatgga atattttgcg aaaaaccaac gattcatcca accaaaaatc
     3601 tatctattta tttggaagca atattgtcgc tatggtttta cagaagggtt cttcgacagc
     3661 attcctcgga tagtcagaaa tggtttttta aaagtattgc gtaaagaacg agaagctata
     3721 caatctgctg ataagaaaga ccgtacagtc tcaaaagaaa aatggcaact agtagtgaaa
     3781 gatatccaat ctggaaatgt gcaagagaca atcgacaaat ggaaaggaaa acattatcgt
     3841 tttactgaag ctcagaaaca agcgttggaa tattatcaga agttagaaga atcactgcgt
     3901 ttcaatgatg aggcaagaaa gtatctgaag gaattactat aaaaaacata ctaagattag
     3961 aggggaacat cattgacgaa tgatgttctc ctctattttc tatttttaga ttatagtgaa
     4021 gagtgaaaga aagttcgaac aaagccttga ggactacgag tccctatcaa aaaccgaact
     4081 tctttactgt cattttagaa tcaggtttac gtatgttggg gccatgagcc cgtgtatagg
     4141 aaattggaat cgaaatgaag cagtaaagtt ctttcaaaac ttaaaaagga ggaaaacaaa
     4201 tgccttacat tcttgcatta gatgtttcaa tgggacacag ctacagtgtc ctatacaaag
     4261 acgacacctg cttatttgaa gacgagattg aacacactca aagaggattt catgcattac
     4321 tcgaagagat ccagcacttg cctgagcgtc cattgattgt ttttgaagca acggggattt
     4381 attccagacc agtcgaaaag ttctttcaag atcatcactt ttcttactgt ttgttaaacc
     4441 cgttagaggc gaaaaaacag atggaagaaa gcaccttacg cagttggaaa acagataaat
     4501 cggatgccca ccggctcgca cagactcatc ttaaaaatca acgtcagcca aaagaagtac
     4561 aaagcaattt ttacttagag atgcgcgatt tagcccgttt ttatcaagaa attgagaaga
     4621 aaatcactcg tctgcgcatg gatttacaca actgccttca attgactttt ccagagcttg
     4681 aacagttctt ttcaaacagg ctgacccctt acgcattgac attgatccgt ctttttcccc
     4741 atccagattt tgtattggca tctactcgga caaaaataaa aaataaactg atcaacgaaa
     4801 cacggaaaaa aatctccgcg aatcgtgccg agcaaaaggc agatcaaatc atccactatg
     4861 cacagtgtgc ctatccagct gttgaaaaag acagtatcca ctgtcaaaaa acgatctatt
     4921 acgccgaact tctccaagat ttactcgaac aaaaaaaagc actggcgaca caaatgatca
     4981 aaaaagccga aggctctcct tgctttttgt tgtaccaaac gttccctggg attggcgcac
     5041 tcacagccgc tttattgctc ggtgaattag gagatatcac acgatttaaa acccataaac
     5101 aattgaatgc ctttgtcgga atcgacatcc gacggtatca ttcaggtaag tatactggcc
     5161 aagaccgcat caataagcgt ggtaatccca aagcacggaa aattattttc ttcaccattc
     5221 gaaatatgat tcgccagcaa cgcgcagctc ctaatcatat tgtcgattat tactacaaat
     5281 taaaaaagca acctatccct aagaaagata aggttgccac agttgcttgt atgaacaagc
     5341 ttctgaaatg tatgcacgct atggttaggg cacatacaga gtacgactat gcgtacgcgg
     5401 tctcagtgga ccattaacta aaccacttac agtataccac ttctttgccc agatcgaaaa
     5461 ttttttacga acggggcttt atttggtgtg ctttgatttt gtcgctttcg tttacgagct
     5521 atttttcttg caccttttac ttgactaatc gtaggaaata gaggctggga cagaagcgtt
     5581 taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt gtgaatttca
     5641 gcttatttcc gaaggagttg cttctgattc cgccgtttat acgtgtttag agcgtgagac
     5701 aaaagtgttc tttacttttg tcccacgctc agttttttta tgcgtgttgt tgtgcgttgt
     5761 agtattcttc ttccattttt tgcatacgtt cacgttcttt ttggttatac caaggtgaaa
     5821 cggtaaatgc aaggacatca ttcagcaagt aaacagagct gttcacgaac atcgccaaac
     5881 ttgcagatcc atgcgtaaat gaaatatacc aaaggaccat ttgagcaagc cctgaagcta
     5941 accaccagta atattggttg ttgtaacgca agaaactgat gataccagca gtcaagctaa
     6001 tagaaaaagc aatggcatct acccacggcc gaggatcatc tgtataacga ccaatcagga
     6061 atcctgaaac aagatagaca aggaaagtac tgatgattgc gataaaccag ctttttccag
     6121 tgaattttcg gatttttgaa gccatattat gattccattc tttacttaac aatacgggga
     6181 tatccaatgt gatcatataa gcaatctgtt caccgatact taaatagttc tttgctgaaa
     6241 acccaacgat aataaaacaa atcgcagaca aaatacctaa aataccgttg actgattttg
     6301 ccgcattgat ggataaaata cacaatacac caagcgtggt accgataaat gtaataacgg
     6361 acagccaagt gatgggacta ttgacaagag tcatgacctg gcagccaaga ctgaaaaaga
     6421 aaagataata attttgttgc ggccagcctt ttagctgatg agctaaaaac tgaaaatatg
     6481 acttcatgta tgaggactcc ttaagacact aaatgtagtg tgttctgtat ttactaacac
     6541 aatctattgt atgc
//
LOCUS       DAZKNM010000138         6338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000138 DAZKNM010000000
VERSION     DAZKNM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.140"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..333)
                     /locus_tag="ACG8M9_002249"
     CDS             complement(79..333)
                     /locus_tag="ACG8M9_002249"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0378967.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(737..1519)
                     /locus_tag="ACG8M9_002250"
     CDS             complement(737..1519)
                     /locus_tag="ACG8M9_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378968.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1584..2420)
                     /locus_tag="ACG8M9_002251"
     CDS             complement(1584..2420)
                     /locus_tag="ACG8M9_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0378969.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2438..3847)
                     /locus_tag="ACG8M9_002252"
     CDS             complement(2438..3847)
                     /locus_tag="ACG8M9_002252"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0378970.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3861..5684)
                     /locus_tag="ACG8M9_002253"
     CDS             complement(3861..5684)
                     /locus_tag="ACG8M9_002253"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0378971.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1956 a         1362 c         1016 g         2004 t
ORIGIN      
        1 tagggatttc taataaccga cataacaaag aaacagtgta atccttacac catttttcaa
       61 taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt tttttaagat
      121 ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt cttttccagt
      181 cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg ttgcttcaga
      241 taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca gttcaacaat
      301 catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg tagataagtt
      361 taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac caagttatct
      421 ttagaaccat ttcagataca tatgaaacaa ttacatatga ttttttcata aaaaaacagt
      481 actgtctagt agtataaaat atacaaattt cttaaaaatt ttttcactaa aatcactacc
      541 tgaaacagtc taaaacactt atatatcaac gtttaatgaa actatctccg tttcatttca
      601 gatacaaata aatatagtcg ggctccaaat ccgaaaaaaa atacccccag tagcttatcc
      661 taagctactg gagatgtata agtacggtca tataacaacg gggtagggta aataaatgaa
      721 aaagatggaa gaatcattat tcaataactt ttttaaatac ttcgttgtaa tcagttacct
      781 gtccgttctt aaataccaca gaataatctt ttttcccatt ttcaagtaac cgaagtggaa
      841 tgtctttacc aacttgctct gtcactgcat tactgatacc tgttagatta gctaataaat
      901 ctgataaaga ttgagcatct gtagaaatct ctgtatcagt atttatcgtg attcctttat
      961 cttcatttac aagcttccaa gtaggtggta tagctgtgta tgtttcgatg aatgaatcaa
     1021 ttctttcatc tatcgtcttc ccagtatcta cttctgtctc attactctta gatgaatcga
     1081 ttctttcatc catcgtctgc tccgtaactg cttctgtctc attattctca ccggaaactc
     1141 tcttcacacc caaacttata tattgtccat ctttagtgtg catagttaaa attcccacgc
     1201 ttttactaat aaattcacta taaccagttc tttcaataga gacttcaatg tatttcacac
     1261 tatcatactc aaaagaaaaa tagacatgta aaagcatttt gttgtttaca tctacataat
     1321 aatcctttga ctctacttga gaccttccta tacggaatac agctttactg tctgatatct
     1381 ctataccagc atccttatta ttaatctgcc aatctccaag tagctcttct ggcaagctag
     1441 tagtttcttg tgaacacttt accacttcac tggtagaggt cgaaacattc acctccgcac
     1501 tgttagaaga attttccaag gaatcaggag ccttagcaca accaacaagt ctttatatat
     1561 tgtgcagccg atattttctt cattcagtac acccttgaaa tatgcagtgt tagataaagc
     1621 agttcgtcct gatttgctga ggcacccatc tcttcttgaa cgtactcctt gattttcaag
     1681 gccgcccgat actctttcgg aaaacgctgc ttcactgaag ccagcaaaaa atcatccttc
     1741 tccggcaact cttccttctt aatcattcgg gcaataaaaa actgtaaatg cgtcacaaat
     1801 cttaagtaaa gcatcgaatc tttatcaacc tttttttcgg gaaacagata tcctagcata
     1861 gccaagatat cctttagtgc tcttaccgag agcatggttt cttccatatt tgcttgttca
     1921 ctttgggcat ttacaatgtg aaacgcaatg tttccagcct cctcttctgg caactgaaca
     1981 ttcatttttt tgttgatcaa ttggatagca tagcagccga tctcaaattc ttttgggtaa
     2041 tacttttgaa tttcaatcag caggcgattt tgaaaaacaa tgcctttttg actgcgttca
     2101 atggcatgct tcaagtgatc aaccaaggta aaaaatagac ttttattcag cttaaagtca
     2161 aacttggcgt tggcataatc gataattcgc ttgacgcaaa taattagatc atccggagca
     2221 gcctcaaaca gtttaatgta atcctttttt agagcatcag aaagtgaatc taatttaaat
     2281 actttttgga ttttgtcagc cggaaccaaa tcgcctttct ttagttgaaa tccaatccct
     2341 ttccccatca aaattacttc tttgccttgc ttatctaagc aaagaattaa actgttgttt
     2401 aacgttttaa ttactctcat tagattcacc gttctctcta agctaagcca tttcttgcga
     2461 ttactttttg ataccaaaag aagctttctt tttttattcg ttttaattct ttaagattct
     2521 catcggttcg gtcaacatat ataaaaccat aacgcttatt gaagccttga tgggtactaa
     2581 caacatcaat cgctgaccaa ggtaaataac caaaaacatc tacaccatct gttaaagctg
     2641 cttgaatagc cagaatatgt ttggctagat aatcaattcg atcctggtca ttaattcgtc
     2701 tatcttcctc aactatgtcg ggtgccccat agccattttc agtaattaaa ataggtaaat
     2761 catagcgatc gtacaatttc ctcaatgtta atcgtagtcc agtagggtca actacccagc
     2821 catatggcgt tttatctaca taaggatttt cagctgcccg gtagacacct tcttctccca
     2881 acatgatctg ttgatccccc gctctagcgg aaacatctga tccgtcattt ttgctggcag
     2941 cgatggtggc agttgaataa taattaatcg caataaaatc gggtttaccg gaggataaaa
     3001 ttgcctgatc ctctgcctca attttaggat ataatccacg atcattcaaa tagttttcaa
     3061 acaaataatt gtattttccc cgaactgcta catctaagaa agaccagcct ctcaatgttt
     3121 cccaattatg ggctgcaatt gcatcttctg gactggaggt agcttgatac atagccgtta
     3181 aattgattgc tggaccgatt tttcctttaa gattcaactg atgatacaag tgaattactc
     3241 tagcttgagc taataaaaca tggtgattaa tttgaaagag ttcttttttt gaaggcagct
     3301 ctccacttgg tggcaaacca atcgctccag gatgaaggat catcgtgttt tgctcattaa
     3361 ttgtcagcca atagttcacc ttgtcaccat actcagtaag caatacctgt acatagtcca
     3421 caaaataatc gatagttgct cgatttaacc aaccgccctg ctcctctaga aaataaggta
     3481 aatcgaaatg gtacatggtg acaatcggtt cgattccctg attcaggcat tcatcaatta
     3541 cttgatgata aaaagaaatt cccttttgat tggttttatt attttcaata tccataattc
     3601 gcgtccaagc gatggaaaag cgaaaagctt ttaagcctaa ttctttaaac agctgtatat
     3661 cttctttata gtgatggtaa aaatccgagg caacttcaaa attagctacc ccttctgtat
     3721 gctgatactt gtcaataata gacggttgtt tcccatcctc ctgataggca ccctctgcct
     3781 ggtaggcact aatggaggca ccccataaaa acttctctgg aaatgctgaa agctgtttgt
     3841 attgcacaaa aatcctctcc ttaactaatt tgcatcaatg gttcgtgttc ctttaagctt
     3901 ccgcttgctt cactaattcc tgcgtaattt tcactgttgg tcactaccac aatcactgta
     3961 tcatcgtagc cagcgtttct aatggcagcg aaatcggcgt tgattaataa ttgtcctgct
     4021 gacacctgat ctcctgcttt tacaaatact tcaaagggtt ccccttttaa attcacggtg
     4081 tcgatcccca catggatcaa taattcaata ccttctttag aacgtagacc aatcgcatgt
     4141 ttagattcta aaatagtaat gacttctccc tcaacaggtg aataaatgct gttttcttct
     4201 ggatgaatgg ctacgccctt tcccaatagt cccttagaaa agacagcatc gttcacttca
     4261 gaaataggac acacggttcc ttttaccgga ttttcaacaa tgactgcttc ggctattgcc
     4321 gtttgctctg attcatcaga agtatcgtca aatccaatta gataagcaat gataaaggtt
     4381 gcaacaaagg cgattacaca cgtaataatt gcgtgaacaa tattcattgg attttctcca
     4441 ataaaggctg gtaaggctgc taagccaggg gaaacaaagg catatctgac taaaccgctc
     4501 aagccagcat acaaaccagc aattgcacca ccaatcatgg aagcaactaa tggttttttc
     4561 agcttcaagg ttactccata gagtgaaggc tcagtaattc ccattaaagc agtgatccca
     4621 gtggacacag ctagctgctt caactctttg tttttagttt tcgcggctac agcaaaggta
     4681 gcacctgatt gagcaatatt agaagccagc attcctggcc cattgatcat ttcagaaccc
     4741 gtactgctca attgcatagt agcgattggc gtcattgccc aagctgtacc cgtaaccacc
     4801 aaaaatggct gcaaaccacc catcaaaaat ggaattaacc aagctgcttt gccgttaatc
     4861 cattcagccc ccattgctat aagatcgttc agcaacgttc caaacggtcc aatgacaacc
     4921 aacgctaaag gccccataat taaaaatacc agcatcggct tagtaaagaa tttaataaac
     4981 gaaggcgaaa ttcgatccgc aaagggctct acataggaca tgatccaaac agtaaagata
     5041 atcggtaaaa ctgatcccgc ataatctgca gctaataccg gaattcctag aaaagaaacg
     5101 gcatcgccag tagaaaaaag ctgtgaaata tttggatgaa gcaaaatgcc agcaacagta
     5161 atcgccaaaa ttgggttgac atcaaacttt atggctgccc cataggctaa tagtactggc
     5221 ataaaataaa aggctgcatc agcaatagta ttcaaaatga tgtaagtttg actactgtct
     5281 gatagtacac cagtcaaagt caagacggcc aaaatggctt ttaccatccc ggcacctgtt
     5341 atagctggaa tcatcggtgt aaaggtggta gaaatgacac taatcaatct tgaaataata
     5401 ctttcttgat tgtctgaaga ggggcccttt ttaattgaag ttcccacttc cttttgaatt
     5461 tccttgaagg ctgtttggac ttcattgcca atcaccactt ggtattgtcc accgctatcc
     5521 accacactaa ttacaccaag tgttttttct aaggctttcg tatcggcttt ccttttatct
     5581 ttgagttcaa aacgcagtct ggtggcacaa tgggttaagc tgaccacatt ttcttttccc
     5641 ccaacctgtt gcaatacatt tcttcctact tctctgtaat ccattattct ctctccttaa
     5701 agcagtaaaa aagaccaagt caaaccacgc cccaatcaac attgaagtgt tagtttgtct
     5761 tggtcttgcc tgcataatca gtaacatgcc aataacgtta ttttgctgta atccaataat
     5821 agcacattat gatagcgcaa tcaacaggga atttaatttt ttatcttttc gtttgaccgt
     5881 atagggtaat ataacagtta gatcacatca actagggtat atctgcgtat acttactgct
     5941 aaaataagta tatctaatat taactatacc agtaaaacca ttcctacata accagcggga
     6001 aattcccaaa atcgtgaaag catcaaaaat gcatataacg gatcacacaa cttcaacaat
     6061 tctgctcttg ggaaaaattg atgaatttgg tgagtaaagg atggaataaa cagcattcca
     6121 acaatatagg caacaatagt gatacttgtt tttttggtaa aaatcagtaa gcaatagcca
     6181 atttcaatca ttccgagata taaaggtatc gatagaatga tttgggctag tattcttaaa
     6241 aattaactag caaatgttta aactgattta tttctaatct acatgtagta agtatttcaa
     6301 ttccaccaat tatcaataaa aataccaaac atatccat
//
LOCUS       DAZKNM010000139         6275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000139 DAZKNM010000000
VERSION     DAZKNM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.141"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..318)
                     /gene="rpmB"
                     /locus_tag="ACG8M9_002254"
     CDS             complement(130..318)
                     /gene="rpmB"
                     /locus_tag="ACG8M9_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0378972.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            588..950
                     /locus_tag="ACG8M9_002255"
     CDS             588..950
                     /locus_tag="ACG8M9_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0378973.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1002..2684
                     /locus_tag="ACG8M9_002256"
     CDS             1002..2684
                     /locus_tag="ACG8M9_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0378974.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2801..4837
                     /gene="recG"
                     /locus_tag="ACG8M9_002257"
     CDS             2801..4837
                     /gene="recG"
                     /locus_tag="ACG8M9_002257"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0378975.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4886..5887
                     /gene="plsX"
                     /locus_tag="ACG8M9_002258"
     CDS             4886..5887
                     /gene="plsX"
                     /locus_tag="ACG8M9_002258"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0378976.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6009..6251
                     /gene="acpP"
                     /locus_tag="ACG8M9_002259"
     CDS             6009..6251
                     /gene="acpP"
                     /locus_tag="ACG8M9_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0378977.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2101 a         1090 c         1428 g         1656 t
ORIGIN      
        1 ataacttcat aatttgttcg tataacaaaa ataaacgcaa aaaaagggta ggaatcaaaa
       61 gtcccaccct taaaaatttt tattaaataa aaaaccgttc actatctgaa cggtccaagt
      121 atcattcaat taaacgcgtt caacttttcc tgatttcaaa gcacgagttg acacccaaac
      181 ttttttaggt tttccgtcga ccataacgcg aactttttgt aagttaggtt taacagtacg
      241 ttttgttgag ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc gacctgtaaa
      301 gtaacaaact tttgccatgg taatttcctc ctttgccttg atttggagca atagaataat
      361 tcagctcata ccagactaac atatcatata gaatgggaat ttgcaagtac attttcatag
      421 atacaattga caattgagaa gaataattgt aaattaaaca aggttatacc tgaaattttg
      481 caaggttttt gagtactttg ggtaactgcc ttttatttca agttgcgcta tgataaaata
      541 tgatagaaga aaaatgggag ttttttaccc gttaaggagg ctcattcatg gctgtaaaaa
      601 ttcaaacgtc agctggaacc attgaaatta ccaatgatgt aattgcaaca gttgttggtg
      661 gcgccgcgac agatgtgttc ggtgttgtag gaatggccag caaaaatcaa attaaagata
      721 atattactga gattttgcgt aaagaaaatt actcaagagg agtagttgtc cgccaagaag
      781 ataatggtat tgctgtagat gtttatacga ttatcagcta tggtacgaaa atctctgaag
      841 tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga ggcattatta ggcgtaactg
      901 ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact gccagactag gcaggagact
      961 ggctggtaac tcagcagtcg atctgaagga ggattatcta ggtgagtgta acagaaataa
     1021 gtgcaagcca gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa gtaaatgcag
     1081 aatacgtcaa ttcattaaat gtattccctg tgccagatgg cgatacggga acaaatatga
     1141 atctatctat gactagtgga gctaaggcag tagtagattc aacctcagaa aaagtaggag
     1201 aattagcagc aatgctttca aaaggattgc tgatgggtgc gcgcggaaat tccggcgtta
     1261 ttttgtctca attgtttcgt ggattttcta agcaaattcc agatgttgtc tcattaaatg
     1321 cttctgatct tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa gcagtaatga
     1381 aaccagtaga aggaacgatt ttgacagtag ctagagaagc agcgaaagct ggagaaaaga
     1441 aagcaaaaag tactgatgat gtcgttgaag taatgacagc tgttgtaaaa ggagcaaagc
     1501 gtgctttagc taaaactcca gacttgcttc cagtcctaaa agaagtcggt gtcgtagaca
     1561 gtggagggca aggactgctg tttgtttatg agggcttttt aagtgcattg aatggagaat
     1621 tccaagcgga tgaaacatat gaaccttctc cagcagaaat ggatgatatg gtcaatgcag
     1681 aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt ggttactgta
     1741 cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga tagtacattc gattacgaag
     1801 aattccgtaa ctatttagat ggtataggag attctttact tgtggtcaat gatgacgaga
     1861 tcatcaaagt ccacgtgcat acagaaaacc caggagaagt cttgaattat ggacaaaaat
     1921 tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag acgattttgg
     1981 aacacgatga agaagtgaac gagtttgcga aaaaagaaat accacgtatc ccgtatggta
     2041 tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta ggcgctaatt
     2101 atgttatcag tggtggacaa acaatgaatc caagtacgga agacattgtt aaagcgattc
     2161 aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa taagaacatc ttcatggcag
     2221 ctgatcaagc agcagaagta gcagatatcc cagtagcagt tgttccttca agaacgattt
     2281 cccaaggatt gacagctatg ctttcattca atgatcaagc ttctttagaa gaaaacaaag
     2341 aaacgatgac agaaatgttg tctagcgttg tgagcggtca agtcactcac gcgatcagag
     2401 atacctcaat tgacggtgta gagatcaaag aaggagactt tttgggcatg atcgatggaa
     2461 aaatcctgat ctctaaccca gatatcacag caacagcgtt aagtacacta gaacaaatga
     2521 tcaatgagga cacagagatc gtaacgatct tggcaggtga tgaaggaaca gttgaacaag
     2581 caaatgaatt tgctgagaaa ctaacagaaa aacatcctga tttggaaatc gagatccacc
     2641 aaggagatca gcctgtctat ccttatctat tttctgctga ataatttctg tgtcagaata
     2701 tctttctaac acagtattca ttcataaaag gccttcggtt agtgatcagg tatgatcgct
     2761 gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt atgcgttctt tagatgatcc
     2821 aatcactatg ctggcaggag tcggagaaaa aagagcagct agtcttgcca gcttgggcat
     2881 ccaaacgatt gaagacttac tgacttatta tccttttcgc tacgaagata ttcaggaaag
     2941 aaatctaaac gagatccagg atcaagaaaa ggttactttg aaaggaatcg tagtctcgcc
     3001 tcctgtgatg aatcgatttg gttataaaaa aagccgattg caatttcgta tgatgcaaga
     3061 acatgatgtg ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa aagtcatttt
     3121 atcggaggaa atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct tgaacgggat
     3181 gaagatttta ggttctcaaa caatggatga cttttcgccg atatatcacg tcaacaaatc
     3241 aattcgtcaa acgacattag ttgatctaat caaaaaaggg tttcaagaat acggcacatt
     3301 gattgaagaa aatctgccaa acaaccttgt ggagaaatat cgactgcttg accgagcttc
     3361 tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag ccaaacgacg
     3421 tgtcgttttt gaagagttct ttttatttca aatgcgtctt caaggattga aaaaagcaga
     3481 aaaagcagaa acgaatggtt tggagatcct ttatgatgtc caaaaattga aacaatttac
     3541 tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg aaatctgtcg
     3601 ggatcttcgc agtccgcagc atatgcagcg acttttgcag ggagatgtag gtagcggaaa
     3661 aacagtggtt gccgcgattg cgttatacgc taccgttaca gcaggtttcc aaggtgcgtt
     3721 gatggtgcct acagaaatct tggcacaaca acatatggaa agcttgcaac agctgtttga
     3781 tcccaatgaa gtaaccatcg cattgattac ggggtcaacg aaagcaaaag aacgacgtga
     3841 gctccttgaa aaactagagc aaggggaaat taacgtcgtg atcggcacac atgcattgat
     3901 ccaagatgga gtcgaatttc agaatttagg tctagttatt acggatgagc agcaccgttt
     3961 tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg cgaccagatg tcttatttat
     4021 gacagcgact ccgattccta gaacattagc tattacagca tatggtgaga tggatgtatc
     4081 tatcattgat gaacttccag ctggacgtat cccgatcgaa acacgctggg taagaacacc
     4141 tcaattggac agtgtactgg attggacgaa aaaagaactg gcaagaggac atcaaatgta
     4201 tgtcatctgt ccgctgattg aagaatcaga agcattagat gtaaaaaatg cagtggaaat
     4261 ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt tacatggtaa
     4321 aatgaagaat caggaaaaag aagtaatcat ggaagccttc aaagacaacc agatgcagat
     4381 attagtttcg actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga ccgtcatgct
     4441 gatcatggat gcggatcgat ttggattggc acagctgcac caacttcgtg gacgtgttgg
     4501 acgcggaagt gatgcttcct actgtatctt agttgctaat cctaaaaatg aactaggtgt
     4561 ggaacggatg aaaatcatga cagagaccac caatggtttt gtactaagcg aaaaagatct
     4621 ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc ctcaatttgc
     4681 gattgctgac ttggtgacgg atggaaatgt tttggaggta gccagagaag aggctgcagc
     4741 cctttggcaa ataaaggatt ggcagatgct tcctgagtat cagcctttag cgcaaagcct
     4801 ccaaatagaa aatcaggata atcgttattt cgattagcag actgtgctat actttatact
     4861 gatgacggaa aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg gcggagatca
     4921 cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag caagattttc cagaagtaga
     4981 attcttactt tacggaaaag aagcagaaat caagaaatac ttaacaaatg aaaaaaacat
     5041 cacgattatc catacagacg aaaaaatcaa tagtgatgat gagccagtaa aagcaattcg
     5101 acgaaaaaag actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg gtgaagcaga
     5161 tgctgtattt tcagcaggga atacaggcgc gctactagca gcagggttgt tcatcgtagg
     5221 acgtatcaaa aatatcgaac gccctggctt gatgtctacc ttgccagtag tagggaaaga
     5281 aggtgccggt ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac cagaacattt
     5341 gttgcaatac ggtattttag gttcttttta tgcacgtaaa gtccgaggta tcggacgtcc
     5401 tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag tgacgaaaaa
     5461 agcctatgaa ctccttcaga atgaaacaag cttgaatttt attggaaacg tagaagctcg
     5521 tgagctactc aatggtgtgg ctgatgtagt ggtcacagat ggatttacag gcaacgctgt
     5581 tttgaaatct attgaaggaa cagcaatgaa tatgatgaat ctattaaaat ctgctattct
     5641 aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta aaagacggct tacgttcttt
     5701 gaaagcagaa atggattatt ccaaacatgg cggtgccgtt ttgtttggct tgaaagcacc
     5761 agtcattaaa actcatggag caacaggccc cgatgctgtt cgctatacga tccgccaaat
     5821 ccacacaatg cttgagacag atgtagttgg acaattagtc gaacaatttg aaaaaagcga
     5881 agaatagtat ttggatatct gaagcgatcc gttccattga aaaaaaggtg gacattcctg
     5941 aaaaatgcca gtaaaattag ccatggtgta ggtaattgac aagtgtatga ttcttggagg
     6001 tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc gaaaattatt tcaaatcatt
     6061 ttgagataga taccgataaa gtgacggatg aattgaacat caaagatgat ttaaaagctg
     6121 attcaattag tattatggaa ttcgttttag agcttgaaga tgaattcggt acggaaattt
     6181 cagatgaaga cgctgaacaa attgaaacag tcggtggcgc agtagattat atcagcagcc
     6241 atcttaaata atttcaaata aaagcaaaga aggat
//
LOCUS       DAZKNM010000140         6258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000140 DAZKNM010000000
VERSION     DAZKNM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.142"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8M9_002260"
     CDS             complement(99..1229)
                     /locus_tag="ACG8M9_002260"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0378978.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8M9_002261"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8M9_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0378979.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8M9_002262"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8M9_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0378980.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8M9_002263"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8M9_002263"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0378981.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8M9_002264"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8M9_002264"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378982.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8M9_002265"
     CDS             4880..5692
                     /locus_tag="ACG8M9_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0378983.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8M9_002266"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8M9_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378984.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1854 a         1285 c          996 g         2123 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaa
//
LOCUS       DAZKNM010000141         6139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000141 DAZKNM010000000
VERSION     DAZKNM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.143"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(350..835)
                     /locus_tag="ACG8M9_002267"
     CDS             complement(350..835)
                     /locus_tag="ACG8M9_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378985.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(974..1189)
                     /locus_tag="ACG8M9_002268"
     CDS             complement(974..1189)
                     /locus_tag="ACG8M9_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0378986.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1413..2213
                     /locus_tag="ACG8M9_002269"
     CDS             1413..2213
                     /locus_tag="ACG8M9_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0378987.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2232..4100
                     /locus_tag="ACG8M9_002270"
     CDS             2232..4100
                     /locus_tag="ACG8M9_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0378988.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4093..4584
                     /locus_tag="ACG8M9_002271"
     CDS             4093..4584
                     /locus_tag="ACG8M9_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0378989.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4572..5126
                     /locus_tag="ACG8M9_002272"
     CDS             4572..5126
                     /locus_tag="ACG8M9_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0378990.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5144..6139
                     /locus_tag="ACG8M9_002273"
     CDS             5144..6139
                     /locus_tag="ACG8M9_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0378991.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2173 a          973 c         1227 g         1766 t
ORIGIN      
        1 taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa taataagaag
       61 aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa aattgaatct
      121 agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa gtagatacca
      181 aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa tagctgtaac
      241 gaattattag gttaatgcaa atgcttattt atactccgta taactttctc agaaaaaatg
      301 ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat caaaatggtg
      361 ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa aaaccaacgc
      421 ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa taatctcgat
      481 caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa cggtgggcaa
      541 taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca gtgctttctt
      601 gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta tgttgcgaat
      661 ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta gttgaaccaa
      721 cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt ttatatccac
      781 aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta tgcatgatag
      841 ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa aagcagatgg
      901 tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata acttttttaa
      961 taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg aaaaatttca
     1021 agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa atagttgttg
     1081 aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac agccagttca
     1141 accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt agaattctcc
     1201 tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat ataacgctca
     1261 ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca ttagtacaca
     1321 ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct tttatactgg
     1381 atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat attgcaggaa
     1441 aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc gaaaatttgt
     1501 tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata cagcatgaaa
     1561 acctactatc tttaaaagtc gatgtgtctt ctaaaacgga catagaagaa gggatatata
     1621 aagtaatgaa acatttcaag acgatcgatg gtctagtcaa taatgcagga ataaatattc
     1681 cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca gaacaagttt
     1741 ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa gcagttggaa
     1801 gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa tcaggattgg
     1861 aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac agttttactc
     1921 gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt gcaccaggga
     1981 tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct tatacgagag
     2041 gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc cctctaggaa
     2101 ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca gaaagatcaa
     2161 gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga taaaggatag
     2221 gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta ctagtcactc
     2281 acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat caaaccataa
     2341 aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc atccaaaaaa
     2401 acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc atgcaaggaa
     2461 aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat ttactaaata
     2521 ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa gtcggtgtca
     2581 gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat gattttgatg
     2641 taaagattac aggcacacca aataaaggac taagaataat aggagatgag ctagaattac
     2701 gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca gaacagtttc
     2761 tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt aaacaagaaa
     2821 tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt aactatctgt
     2881 tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa ttcaatgaac
     2941 tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa aaagatttta
     3001 tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa gctaactctg
     3061 atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat ctcattgatc
     3121 tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg ataaatcggg
     3181 taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa aagtatcctt
     3241 tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg caacataaga
     3301 tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt cgatatcaaa
     3361 aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa ggaacagcat
     3421 taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata acacatgttt
     3481 cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt acaacgattc
     3541 cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt tttgatgact
     3601 cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg tttcattcta
     3661 ctgttttttt ctatcaaata tggaaagaaa aaaagacgta tcaagaatat ttaaacaaat
     3721 tagtcgctcc tttgatcaga ggctattatg tggatgaaga atttgaaaaa aggatcttga
     3781 aaagagaata tcagcaaagc acatttgtta acaaagaatt tgcttttcct cacacaacca
     3841 accagctatc tcaacacatt gttttatcag tagggctaat aactgatcaa ggtttagcag
     3901 aaacaggagg aaatggagtt atttttttat ttgccatacc tgaagaaatg acggctgaag
     3961 ttgagaatga attatttgag ctatacgata tcctatttga actcataaaa atgggaatag
     4021 atgagtttat tgaattagca ccgacacaag aatcattttt aaaactaatc aaaagtaaag
     4081 gagagagact tcatgaatga tatgtatcta ttcatagttt tcgcagcttc agcttatgtg
     4141 ctgcaagcag ttatcagttg gaagcaacta aagcatttca atcaaacata tagcgaactg
     4201 cgaagaaaag gaaaagtagc gattggtaaa cgacctggga aaatccgttc aggcacgatt
     4261 gttatgctag ctatagacga tcatgatcaa gttttagaag tcagaaaaat gcaaggggtt
     4321 acatctttgg caaaatttca ttcattacct gtctatgcgg gtgaagatat ccattacatc
     4381 gatcgttacc atccctcagt tcgagcggaa gatcgtttga cacaagcagc tttagaaaat
     4441 gccagagaag tatacttacg aatagaggca ggaaatgaaa aaagtgaaca accttcattg
     4501 ccttttattg ctatgctcaa taattttcgg atatggaaaa gtacgatcaa agaaaaaagg
     4561 agtgacaaac gatggatatc ataacaaaat ttgctgaggg atttatgagg ctgttccaaa
     4621 caggtgcaga aacattcata agctggatga caggaatcgt accagttgtc ttgatgctga
     4681 tggttgcaat gaatacgctg attgcttttt taggtgaaga ccgagtaacg aaagtagcca
     4741 aattatcagc gaaaaatccg cttactcgtt accttgtgtt accgttctta tctgctttta
     4801 tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct tccagaatac tacaaaccaa
     4861 gctattatgc tgctcaagct caattttgtc atacgagcaa tggtgtattc ccccatatta
     4921 atcctggaga attgtttgta tggatgggga ttgctcaagg gatcgaaacc ttagggctaa
     4981 attcgatgga gctggctatc cgatacttgc ttgtgggact gctgatgaac tttattggtg
     5041 gttggataac tgattttact actggatttg tatgcagaca gcaaggaatc gttcttagca
     5101 aaaaagtaga gctttcagtt gattagagaa aggagagttt taaatgtatg gtgatattaa
     5161 gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt attaggccga caaaagataa
     5221 acacaagttc atctatgtca caggtggagg tgagaggcca atgatcgtag ataaaataga
     5281 agaactgaca ggtatggagt ctatcaatgg gttcaaaaca tcaatacctg atagtgaaat
     5341 cgcgcttgca atcgttgact gtggagggac tcttagatgt ggaatttatc ctaaaaaagg
     5401 gataccaacc atcaatatcg tttccacagg aaaaagtggc ccattagcac aatatatcac
     5461 tgaagatatt tatgtttcag cagtagggtt agaacaaata cagccactaa tggaaactga
     5521 tgataacaaa gaaacagtaa atactgcatc tccagataaa aagtacaaat atgatacttc
     5581 taaaaagata accgagcaaa aagcagagca gcaaaactgg atagcaaaaa ttggaatggg
     5641 cgcgggaaaa gtagttgcta cattcaatca atcagcaaga gaagcagtag atacgatgat
     5701 ccggacagtg atccctttta tggctttcgt ttcccttttg attggtgtga tacaaggttc
     5761 tgggatcggt aatgtatttg ctaacattat ggcaccttta gcaggaaatg cagttggatt
     5821 aacaatcatt ggctttattt gttcactgcc atttttatct cctttgcttg gtccaggggc
     5881 agttattagc caagtgattg gaacattgat cggtgtagag attgggaaag gaaatattcc
     5941 tccccaatta gcattaccgg cactatttgc tatcaatact caaaatgcat gtgattttat
     6001 tccagtcggt ctaggtttgg aagaagcaaa agctgagaca gtagaagtag gtgtaccatc
     6061 agttctttat tcaagatttc ttaacggcat cccacgagtt atcattgctt gggtagcaag
     6121 ttttggatta tatagctga
//
LOCUS       DAZKNM010000142         6114 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000142 DAZKNM010000000
VERSION     DAZKNM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.144"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1222
                     /locus_tag="ACG8M9_002274"
     CDS             194..1222
                     /locus_tag="ACG8M9_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0378992.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1248..1688)
                     /locus_tag="ACG8M9_002275"
     CDS             complement(1248..1688)
                     /locus_tag="ACG8M9_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0378993.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1848..2354
                     /locus_tag="ACG8M9_002276"
     CDS             1848..2354
                     /locus_tag="ACG8M9_002276"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0378994.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2522..4435
                     /locus_tag="ACG8M9_002277"
     CDS             2522..4435
                     /locus_tag="ACG8M9_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0378995.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4440..5441
                     /locus_tag="ACG8M9_002278"
     CDS             4440..5441
                     /locus_tag="ACG8M9_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0378996.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5643..5849)
                     /locus_tag="ACG8M9_002279"
     CDS             complement(5643..5849)
                     /locus_tag="ACG8M9_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0378997.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2261 a          934 c         1214 g         1705 t
ORIGIN      
        1 tggattcact ttacaaaggt acttatcaat ccttagaaaa tcttagtcaa aaaattatat
       61 aagtttgcgt acgattcaaa gaaaattaag agaacttaat actattttga aaaactatca
      121 tttatcttgc agcttgaaaa aaggtaatcc gatagaagga gaagaatacc gtatccgata
      181 cttttttcac ttgatgtatt ggcagtcatt cgacgaaaat cattctgata tattatcaag
      241 tataaaaaat aaacgtcaac tattagaaaa atttgcgaaa catgcgccat atacgcgtag
      301 tatagataga aagaaatgga tcagcctact aagaatttcg ttgcaacgaa tgaaacaagg
      361 ccactcgatt acgtatgtcc cagaagagat gaacaaattt gtgcatccat tgataacaaa
      421 agaagagttt acgcattaca tactagagcc tttttttgaa gcaaacttct ggtcgatgaa
      481 tcacatcact ccaacagagc aagcttacct ttattttatg tttggtgtaa tgaacaccta
      541 tttggaagaa gatttgttgg atactccaga taaagtacaa gaaagttggg gctggattga
      601 atcgtctatt gtagaaaatt ttgaaaaaaa cttcaagatt tgttttacaa cttcagaaaa
      661 aaattatcta tatgtcaatt tggcaatgat ccatttgtat tcaagggttt ttgggacaaa
      721 aaagaaggtg gatgcttttg gaaaatcagc agaagacaga gattttcagc atatatttcc
      781 agtgatgtat ccaatcatgc aacagttttt tagtggggta gctcgcagaa ttccagagct
      841 taatacttat tatgaaaaga acagacggtt gatttttcaa tactgtatgt tagtcaggga
      901 tatttttata aaacacgaac aacctgtcat attctatatt caatcaaagt atggtaaaac
      961 acaggaatta tgggcaaaga aaaggatatt atctatgact gaacgcccaa taaaatatag
     1021 tgcatccgct gatcagttac ctgatattgt cattagcgat tatcctatcg acaaaaaggc
     1081 atatgatcaa acgaatacac agatatttta ctggaatgga caacctacaa aaaatgattg
     1141 ggcgcgtctg aaagctgaaa tcataaaaat caggaatgag aaaaatagat ccatattgga
     1201 aaaattggat ttcgttaatt aagaaaagca gattgtttat gccaaaatta agacattgca
     1261 aaaagattat tttctttaaa atcgacaaga tgaccagaaa gcaagagttc gttgagctct
     1321 tgctttcttt tagttttaga aaggaacatc ctgattttat cttcgctaaa tccagtcaaa
     1381 agttttgcag attggtcgta gataatcggt tttttcaata aagaagggga tgtacgaatc
     1441 tcaatatata gttcactcaa actgagttca tctattctgt gaacaagatt tttataacta
     1501 atagaatgag tagagagcat ttcggtcacg ccgttgtttg tcaattttag taagtggata
     1561 aattcatttt tggttagtgg ttgacgaatt aagttctgtt cgataaacgg tatatcatgg
     1621 acgataagcc aacgttttgc gcgtcttgaa gacgagcagc cagaacctgt atacaattta
     1681 atcatcatac attcctccaa tagctaagta tttatgaaat aataacatga agaaatagtc
     1741 gaaaagtgcc atttagaggt gtaaaataag aaaatgttgt cacgtataat ggaactagct
     1801 agataagaaa tactattaaa tcaagaaaaa agaaagaagg cacaagtatg tcaaacacag
     1861 ccgacgcaat agtagtgatc gatttacaaa aaggggtatg ttttgattct tcgccgatac
     1921 acgaattaga aacagtgaca gcattagtga ataaaagaat caaaacatac gaaacgattg
     1981 ggaaaccagt tattttcgtt caacacgaag atgctcaatt agtgccacaa acaaaaccat
     2041 gggagctatt gtcggaatta tctattcctg agcacagtta ttttgtgaga aaaacccatg
     2101 cgaattcatt ttataagact aatttgagtg ttttattaac tgaattggac tgtcattcga
     2161 tcgaattatg tggggcacaa gcagagtatt gtatagatac gactgtgaag tttgctcatg
     2221 gattaggata ccagcttatg atgtgtagag gagcaacgac aacttatgat aatcctttta
     2281 tgaaagcaaa ggaaacaatc gctttttacg agaaaatttg ggataaacga tttctgacat
     2341 ttttggagca ataagttgtt tcttcgttta aaaaaagcag aatggatact ggtttatcca
     2401 ttctgcttta ttacgtaaaa tgagaaaata atcctgttct atttttatgg gataaaccat
     2461 ttattttttg tataaaaaat gtttgcgttt acatataatt ctcctagaaa cataaaaagg
     2521 agtgaatagc ttgagaacaa aacaagtatt aaagggagcg ttggtcatct tatttgctgt
     2581 aagtacaagc gtcccgcaat ggacagtata tggagagagc atttcttccg aatcggaatc
     2641 tcgggaggta gaaaccacag aaactactga tgctggaaat gaaacgaaat cgagtacttc
     2701 acaaacagaa acaaatactg aaacaacggt tgagacatct gttactagca cagatcaaga
     2761 aacgaccgct accacagata accaacaaac agaagaagca acagctacaa aaatgtcaaa
     2821 cgacgaatcc tcttctgcta aagtaagtga caaagagaat gggacagaaa atgatgatat
     2881 cggtaaaggt gaaaatttca atggtaaaga atatttgaat tacttcggta aacagatgac
     2941 agaaatactt aatggaccaa cagaagacat caagtcaaaa ttaatagagt tattagtcac
     3001 tgagcttgct gttaatgatt atggtgtaga taaagagatt atcaaacatc tgattgctac
     3061 ttatctattg tacgatatcg cttttgcaaa aaacagtgtg ttgatgtcga tgcttggagg
     3121 tacagatgat gggacaacgc ctacagaaaa cacaggcttc tatccaaata caaaacaact
     3181 tcatcaaact gtactaaaca aacaagatgg caaaatgatg gatgtatatg catattatgt
     3241 cgatcaaggt tcagataaaa cagtcatgat ccatggcgga tttcgaggga attggaataa
     3301 cgggatcgtt acagaagaat acaatgattt ttataaggcc gggtataatt tattatttgt
     3361 tgattccaga gcgactggta atagcggagg cgattatgtg acctatggac aatatgagtc
     3421 agatgacgtc ctttactgga taaatcaaga agttcgagag cggccatctc aaaagatttt
     3481 actttatggt ggttcgatgg gagcagctac gatgatgtct gtattagcga aagatattcc
     3541 tgtcaatgtc aaaggaatta ttgaaaattg cggatttgca tcaattgatg aacaactacg
     3601 ttttacgtac agccagacag tagcccccgc actaccggat gccataaaaa atcagttaga
     3661 catcattggg gatcaagagc atgaagattt atttatgggc cttttgaaac aatactattt
     3721 tgatcaggag atgcatttgg atacaaaagc tgctctacct acgattggaa tgtcagattc
     3781 attgcctaaa ttgattatcc atggaacagc agatgatgta gtacctgtgt ctaacgcgca
     3841 aaaattatat gagttatcag gagggtataa agatctttta ttagtagaag gagcagggca
     3901 tggaaaggca caggaagttg accatgctgc ctacacgaaa catgtaacgg actttttgaa
     3961 agtagtgttc catgatcaga taaacgtaaa gtatgtggat gaaaacaata aaagtctact
     4021 caaagaccag gacgaaatcc agctttacgg agcttatgga gaaaattata tgacagagca
     4081 aaagacattc gaaggatacg aattagcaaa tgttgaagga ccgactgaag ggatttttaa
     4141 tgagacgata ccaactatta ttttctatta caaaaaaatc ccagtagctc caccaaaaaa
     4201 gcaagatccg acagagaatg cagacaataa gggaaagaat gaacctcgag aggatcaatc
     4261 aagtgatgat acgcaaccga ttcagatgac agtgaaagta caaaaaaaga cagatgcaaa
     4321 aaaaagaatg ttgccgaaaa ctggagaaaa gaaacattcg cttctcattt attcaggtgg
     4381 atttttatct gttagcacga ttatcacatg gatttggcga agaaagagag ggtaaatgaa
     4441 tgaaatggtt caagcgaata gtgattgcag taccagttat catcgtagtt attttagtca
     4501 ctgtattttt gattaatcaa atcacaccaa agccgattag cttgcttgtt agaaaacaat
     4561 ttgacacaag tgaaaaagaa caagtatatg cccgaccaga tgatttccag caaaaaatcg
     4621 aacaaatcga tataaaaaaa gaaatatcct atccttcaaa gtacaaaaat aataatatgg
     4681 atatctacac accgaaagtg gaaaagaaaa aattaccgat tcttttctgg atgcatggag
     4741 gcgcatatgt aggaggggat aaaaatgatt gtcgtgatta tttggaatat ctatgttcag
     4801 acacgcagca aatcatcgta aatatagatt atgaacgatc accggaagca aaacatccta
     4861 caccagttat ccaattgaac gaagccattc aagcagctaa aaaagagata aaagatcaag
     4921 cagattggtc gaatatttcg attggtggaa attcggcagg tgcacaaatt agtggagaat
     4981 atttgttagc acttcaaaat gaagaaatta aaaaagctga tcatcttgat ccgactttgg
     5041 agaacaagca gattacgaag tttatttctt tgtctggtct gttggatcct tctgcactta
     5101 ctcaagtaag tgataaaatc tctagctttc tttatgaaaa atgtggatgg gcttactttg
     5161 ataataagga ctttgaacaa tcagagaagg tcaaaagttt agccttggtc agacatgcgg
     5221 atcgttggac gcaaaacaca ttcttaacgg atggaaatac aaatacgttt acgaaacaaa
     5281 tggaagaagt aacttctgta tttgagaaag cgggggtgaa agtcacaaaa gtagattatg
     5341 cggagaaaaa tgctgttttg aaccatgaat atcagtttga tttcagcaat aaacaagcgc
     5401 aagaaacata tcaaaagtta gtaatgtttt tcaaggagta aagtcttatg atgcaaaaga
     5461 ggttgtaatc tgtcacttag ctccaagtca caaggagcaa tttcaataaa caagcttttc
     5521 atggtttatg aatattgtaa cttgtgactg aggagttaga gatggcaagg ggcacctttt
     5581 attcgaaatt tgggaatatc acacgattat tgtcacgatc tcttttacgt aaattttata
     5641 agtcagattt gttgttttga tcgtttaaaa aaagcaacaa atccgaagcc tgcggaggca
     5701 agaagggaca aaaagccgag tggtaagcaa aagaatgctg gttccactaa tattccgttg
     5761 ttattgatat aaggctggga acaaagatga atagcaaata aaatcaatgc actagcgaaa
     5821 aaaagcaaag aaatatggat agctttcata agcagcacct ccttttttat aattgtatga
     5881 taaatagatt aagaatctat aaatgtgaca agtttgttag gatgtgaata aaatgatcat
     5941 ctagcgaact tgaaacaaag atggctaatg ataagcaatt aatgattcag caatcagata
     6001 ggtacaaagt ttaccatcat acgtctagac caataataga aaatatggga atataataga
     6061 aaagatttag gataccccca aaagaatttc cccaaaaatt cgatgtatcc taaa
//
LOCUS       DAZKNM010000143         6066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000143 DAZKNM010000000
VERSION     DAZKNM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.145"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8M9_002280"
     CDS             complement(254..541)
                     /locus_tag="ACG8M9_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0378998.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8M9_002281"
     CDS             complement(561..785)
                     /locus_tag="ACG8M9_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0378999.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8M9_002282"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8M9_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379000.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8M9_002283"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8M9_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0379001.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8M9_002284"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8M9_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0379002.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG8M9_002285"
     CDS             3560..3646
                     /locus_tag="ACG8M9_002285"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0379003.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG8M9_002286"
     CDS             complement(3767..4456)
                     /locus_tag="ACG8M9_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379004.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACG8M9_002287"
     CDS             complement(4463..4741)
                     /locus_tag="ACG8M9_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379005.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACG8M9_002288"
     CDS             complement(4701..5615)
                     /locus_tag="ACG8M9_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0379006.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG8M9_002289"
     CDS             complement(5597..5977)
                     /locus_tag="ACG8M9_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0379007.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DAZKNM010000144         6013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000144 DAZKNM010000000
VERSION     DAZKNM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.146"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1078
                     /locus_tag="ACG8M9_002290"
     CDS             <1..1078
                     /locus_tag="ACG8M9_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFQ0379008.1"
                     /translation="LKVTSERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSY
                     MFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARL
                     PDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDE
                     YFKDSQIDIVNRFIDFVEITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKD
                     HLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELR
                     QQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYD
                     KLQRDPKTGEKFQIFAKGVKEPKK"
     gene            1194..3716
                     /gene="pglZ"
                     /locus_tag="ACG8M9_002291"
     CDS             1194..3716
                     /gene="pglZ"
                     /locus_tag="ACG8M9_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0379009.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            3834..5867
                     /gene="brxL"
                     /locus_tag="ACG8M9_002292"
     CDS             3834..5867
                     /gene="brxL"
                     /locus_tag="ACG8M9_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0379010.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(5822..>6013)
                     /locus_tag="ACG8M9_002293"
     CDS             complement(5822..>6013)
                     /locus_tag="ACG8M9_002293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379011.1"
                     /translation="TAPNDSFWNHDQGRTYTKIFCPFDPNRLTTNLVSNIDWYVVKPA
                     NGKVVYSTPRALKTASSGV"
BASE COUNT         2095 a          825 c         1209 g         1884 t
ORIGIN      
        1 tcttaaagtt acatctgaga gaaaattaaa aaaaataaga gaaaaagtgg atgaaaatat
       61 cgatatagct agaaaagact ataatgctct tgaaacgtca tacgagttta gaagaaacat
      121 tttgttaagt tatatgttta aaaaagatct ctcagaggca tttaactgtc taagtaggca
      181 gttggttaat caatttaata ctctaaaatc gaatgaagag gaactgaacg acatattcat
      241 taatcattat tctcttgaaa aagaattaac aaaagaggtt caagatgaat atatctctgc
      301 aaggttacct gataaggcga gggaaattaa ggattttatt tcttattcaa taggttgtat
      361 cttcggtcgt tactccctag acgaagatgg tctcatcttc gctggcggcg aatgggatcc
      421 ttcccgctat caaaaattta aacctgatac tgataatgtt ttactcatca ccgatgatga
      481 gtatttcaag gatagccaaa ttgacattgt caatcgtttc attgatttcg ttgagattac
      541 ttttggtaaa gaaacccttg aagaaaatct ccagtttatc gctgacactt taggtggcaa
      601 agggacaagt cgagaaatta ttcgtcgtta cttcgtacaa gatttttaca aagaccattt
      661 aaaaacatac tccaatcgac caatttattg gcaattggac tcaggaaaac aaaatggctt
      721 taaagcgtta atgtacttgc atcgctatac accagatcaa ttaggaaaag tacgtactga
      781 atacctacac gaactacaaa aagcgtatga caatcgaatc gaattgcgtc agcaacaaat
      841 ttcaacaagt gataacccaa aagaaagaaa tgcaatgaca aaagaaattg taaagattca
      901 gaagcaatta aaagaagttc gtgagtatga cgagaagttg ggtcacttag cattaaaacg
      961 aattgaatta gacttagatg atggtgtaat tgtcaattat gataagttgc agcgtgatcc
     1021 taaaaccggc gagaaattcc aaatttttgc aaaaggggtc aaagaaccaa aaaaatagtt
     1081 atctaagaaa gttgttttct aacaactttc ttttttattc ttaaattgcg tgcgaaatta
     1141 tccaaattat gtcaatataa ggggtataga aagagaacga ttaggtgaga ttgatgacag
     1201 atataaatac gcaacaaatt gaagaaaaat taaatcaacg ttttcctaat tatggatgta
     1261 ggaaaatcat tttttggttt gattcaaatc aggattttct tgaagaaatt gactcattgg
     1321 tcattcaaaa tgcaaaaatc tatcgattag aaaaggacgc acaatttaaa actaagcgtt
     1381 tattggaata tgaagatccc gaaactaact atttaatcta tgcgccattt cctcgttggg
     1441 atgataaaga tgaagataat catttgttga gtgtattgaa ttattcagag gaatttagtg
     1501 cagaccgctt agcgattatc atgaatgaat tggctattcc acttagattt catcagacag
     1561 ttaaacagta tcgtaatttc tttaatgcaa aggatcgcat gagaagtttt ggtgaattta
     1621 gtacgcatat gacaattaat aaatcagaaa cgattgaatt aatgataatg gctgttttgt
     1681 tgaaatcgaa aagtattcga ttaaatgatt tactgcgtgt agttctaaaa agctttacag
     1741 aaagtaggga gaatccttta agggaacttg ataagtatca gttaacagct aaattttggg
     1801 aatttattca aatgaaattt ggctttacgg atagccagcc aacattggaa aagctgacga
     1861 tgtgtcttta tttgaactat ttttattatc aaattgatca agagatgccg aagaagttca
     1921 aaggatttga aatactacat ttgaaagcaa acattattac tttcatggat cagtttatga
     1981 atgatacacg ttacatggaa agctttgaac agctatctaa aaaagtatat caggctattg
     2041 atggtgatgg attattagac agtatagaaa ttgatgatct aattgaagca gacgtatttt
     2101 ctgagattca tgcaaaaata ttaatgtttt atgcagaacg attaaaggct tctgacacta
     2161 caacgaaaat tgcaggacaa acaatttttg atagtttaaa gcagcgcgaa aaaatgcatt
     2221 tcgcatcgga ttatatttat gagtatcaat tattgaaaca tgcttattat cttgtggata
     2281 aaaacagatt gtgcttgccg atgaacatgg ataatttgct aactcaatat gagaaggaaa
     2341 tgtattgggt tgatacgcat tatcggaaat ttatttggaa tagtgatcat atagaagaaa
     2401 agcaaaaata tggccaattg tatcgattag ttgaagcaaa ttattcgaag tttcttgatc
     2461 aatcaggaca aatttggaat caaacttttg aatttaagaa tcgtccatcc attcgaaaat
     2521 tttataatca agctgtaatg aaacgctctg ttaaaactgt agttattatt tcagatgctt
     2581 ttcgatatga gttagggatg aatttagaac gtaagttagt tagagaaaag aagtattcaa
     2641 caaacatggc tagctttctt tctgtattac cttcagttac tgaattcggt aaagccgcct
     2701 tattaccaaa caatcaacta acttacaatg aaggaacaga agtactgatt gatgggaaaa
     2761 aaacgaatgg tttatccaat cgaaatgcga ttctccagca acgagatgca aatgctgtag
     2821 ctttaaatta tgatgatgtg gttaagatga ataaggctga ggtacgagaa gtatttaatg
     2881 gcaaatcagt catttatatt tatcacgatc aagtggatag aacaggtgat cactcgaatg
     2941 aaaatcgagt ttttgaggcc tgtgatttag cgataagcga aatttcccga cttattaatc
     3001 gcttgcataa tgatggcagt atctatcgtt ttatagttac ggctgatcat ggatttattt
     3061 ataaaagaag ccacgttgaa gaagtagata agattgaaaa tccatctaga aatgaaaaag
     3121 atcgtgtaga acgtcgtttt attcttagtg aagaagaata cgaaataatt ggtacgaaaa
     3181 attactcttt gggagattct ttaggaaatg atgacactag aaacatttgt gtcccgatta
     3241 cttcaagtat ttttaagaaa gctggcgggg gacaaagcta cgttcatgga gggtcatcta
     3301 ttcaagaaat gctagtccct gttctagaaa ttacggtagc caaaggtagc aatgcaaagg
     3361 aaccagtagc aattgaatta atgactagca accgcagaat tacaggatta tcaaccacgt
     3421 tagaattcta tcaaaaaaat gcaattagtg atgtttatga ccccgcatct ttctggcttt
     3481 attttgaaga tgaatatggt gaacgtatct caaatgaaga aatctatgtt gcggattcaa
     3541 aaacagaaga tgcaaatcag cggtttacta aatttgtttt tgaatttgta aatcgtcgat
     3601 atacagttga agatacttat tatttagtga taaaaaatca aaaaaatgat ttgaccgagc
     3661 gaataaaatt cattatagat aatccttttg cagaatcatt tgattttgat atataaaaac
     3721 gaggtatcca caattttttg tggatacttt ttcttttgta ttatcaagcc tactggttaa
     3781 gtatgatata ctttaattgc tattataatg actgaacaca tggaggaatt ctagtggaac
     3841 aacaggaaat tttaaagtta tctgaattct ttcccggaaa gattgtcaga aaagatttaa
     3901 caaagaaaat taaagaaggt gcgaatgtac ctgtttatgt attggaatat ttacttggta
     3961 tgtatgcttc gtcattggat gaaacattgg ttgaaaaagg tgtggaaaat gttaaaaata
     4021 ttcttgctcg aaactatgtg cgtcctgatg aagcagaaaa agtaaaagca aaaattcgtg
     4081 aattgggtgg ttatacagtt attgataaag taactgtaca tttaaatgaa aagaaagatt
     4141 gttatgaagc agaattctct aacctgggat taaaaaatgt tcaaattagt gaagagtatc
     4201 cgaaaaaatt tgatcgatta ttaggtgggg ggatctggtg tattgtttcg atggaatatt
     4261 ggtatgatga gaatgatcgt tggaatagcc cttttattgc aaaaaaggtt gatccaatcc
     4321 aaatgcctgg tttagacatt gaggcttaca agatggctcg caaggagttt actacaaatg
     4381 aatggattga cttacttcta cggtccattg gtatggaacc tacacaattt aataaaaggg
     4441 taaaatggct tcatttagca agacttattc cattaattga aaataatttt aatctctgtg
     4501 aactaggacc acgtggtaca ggtaaatcgc atgtatataa agaaatttca ccaaactcaa
     4561 tccttgtttc aggtggacaa acaacagtag caaacctttt ctataatatg gctagtcgta
     4621 caatgggact tgtaggtatg tgggactgtg ttgcttttga cgaagttgct gggattaaat
     4681 ttaaggataa tgatggcgtt caaatcatga aagattacat ggcttcagga tcttttgcta
     4741 gaggcaaaga agaaaagaat gcttcagcat caatggcttt tgtaggaaat attaatcaaa
     4801 gcgttgatgt aatattgaaa acttcgaatt tatttgagcc atttccggag gttatgggga
     4861 cggattcagc ctttttggat cggatgcatt gttatttacc aggttgggaa attcctaaat
     4921 atcgaccaga tttttttaca aatgactatg gcttcattac agattatttt tctgaagtca
     4981 tgcgtgagct tcgaaaagtt agttttggcg atagttttga taaatatttc aaacttggtt
     5041 ctcaattaaa tcagcgggat acgattgcag ttcgtcggat tgtatctggg atgatgaaac
     5101 tgttgtatcc tgatggtaga tttgaaaaaa gtgatgtcga agaaatacta agatttgctc
     5161 ttgaaatgcg tcgtcgtgta aaagaacaat taaagaaaat tggtggaatg gaattttatg
     5221 atgtcaattt ttcatacatt gatttagaag aagagcgaga agagtatgtt tctgttcctg
     5281 aacaaggtgg aggaactttg attccagaag ggttagggaa gccagggcat ttgtacacgg
     5341 tatcttatgg caaaactggg caattaggta cctataaatt ggaaacgcaa atgacttcgg
     5401 gaaatggaaa gtttgaacgt actgggatca attcagatgc tggagcaaga gaagcgatgg
     5461 attcggcata ccgatattta agagctaatt atggaagtgt tagtcagtct attagtttga
     5521 cgacaaaaga ttttgtcgtt catgttcaag atatgaatgg cattggtatg acaaaaacat
     5581 tatcgttatc aactataata gctttatgct cagtttcctt aaataaacct gtattaccaa
     5641 gtttagttat tttaggggat attacaattg gtggttctat gattaaggta gataatttag
     5701 ctaactgttt acaagtagca aaagatgccg gagcgaaaaa agtacttgta cctatgataa
     5761 gcgctgctga tattggaact gtacccccag aattaatggg tacctttaat ttgatttttt
     5821 atcaaacgcc ggaagatgct gtctttaaag cccttggggt ggaatagaca acttttccat
     5881 ttgctggttt gactacgtac caatctatat tacttactag attagtcgtt aatctattcg
     5941 gatcgaatgg gcaaaatatt tttgtgtatg tccgtccttg atcatgattc caaaaagaat
     6001 cattgggggc tgt
//
LOCUS       DAZKNM010000145         5989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000145 DAZKNM010000000
VERSION     DAZKNM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.147"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..1494)
                     /locus_tag="ACG8M9_002294"
     CDS             complement(43..1494)
                     /locus_tag="ACG8M9_002294"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0379012.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1815..2579)
                     /locus_tag="ACG8M9_002295"
     CDS             complement(1815..2579)
                     /locus_tag="ACG8M9_002295"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0379013.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2607..3305)
                     /locus_tag="ACG8M9_002296"
     CDS             complement(2607..3305)
                     /locus_tag="ACG8M9_002296"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0379014.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3317..4096)
                     /locus_tag="ACG8M9_002297"
     CDS             complement(3317..4096)
                     /locus_tag="ACG8M9_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0379015.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4112..5056)
                     /locus_tag="ACG8M9_002298"
     CDS             complement(4112..5056)
                     /locus_tag="ACG8M9_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0379016.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5102..5881)
                     /locus_tag="ACG8M9_002299"
     CDS             complement(5102..5881)
                     /locus_tag="ACG8M9_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379017.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1631 a         1250 c          999 g         2109 t
ORIGIN      
        1 tctaccactt tagtaatatt ttctatttat aacactttcg tcctacattg atccccccct
       61 ctgaagtaca gtttttactg tcttaaataa aattttaaaa tctaacccca tactccaatt
      121 ttttatatat tccgtgtcaa gttttgctac ttcttcaaaa tcagtaatat cacttctacc
      181 acttacctgc cacattccag taattcctgg cttaatagca attcttgcac gatgatgata
      241 atcatattct tcatactctg atttaaggca tggtcgaaat cctataaggc tcatctttcc
      301 tgttagaacg ttgaaaaatt gagggaattc atctaaagac gtggatctaa taaattgacc
      361 gattcctgtc ttttttgttc catctggaag tattttattt ccgataattc ttggatcaaa
      421 ctccatctta aacataagat tattgctcat cttattttct ttcattaatt ctacttttct
      481 ttcttctgca tccatataca tacttctaaa cttatacatc ttgaacggtt taccattctg
      541 acctatacgt tcttgtgcaa agaaaattgg gcctggtgaa ttaatataaa taagtggagc
      601 aataaagata aaaataattc ccgttgcaat acatccaaca atacctgcaa gaatatcaat
      661 tgttcgtttt ataaatgcct gttgagttgt cataacattg atacttgttg taagtactgt
      721 atattctcct atacgaccaa tagattgttt tcccaaagca ctatctgata cttttgcaag
      781 attcaaatgt acagtaaggc ccatttcaga gcatcgttca ataaattctt tcatacacga
      841 agtactttta ttcaaaataa taaagacttc atctacccat ccctgacata agtaatccgc
      901 tgcatttttg gaatcagaaa caactgtcac tccctcaaca tcttcaccaa tcatgctttt
      961 atctatgata atgacaccat ttattttata cattttatag ttatgctctt tcacattacg
     1021 aataactact tcagcaatac tactagatgt aactataata agtgatttgt tcccctcatt
     1081 tttcatttta tgtttcaagt aatgtttcca taagaggcga gttatatatg taattacaac
     1141 gtataaacct cccatcaaga aaagaacgac tctagaataa tcatttccac cctgaacact
     1201 aatgagatac aaacttccta acagcaccaa cataatcgct tgctttacag aagaagcaaa
     1261 ttctatatag taccctcttt ttaatacacc tctatatgct tcaaacatga aaataactaa
     1321 aagatctgcc agttcaatga agactgccat gttttgatac attggaatca cataaggatt
     1381 ccaatctacc tggcgtaaga catacgatag ataaaaagca agctggatac acaataaatc
     1441 taaaataata aaatcaatat gtttaaccca tccccttgaa cttttcttgt acattccttt
     1501 ttactcccct ctttttaata aaataaattg tattacttgt tttctctcct aaatatcttt
     1561 ctgaaggatt attgactcga caaattggct tgtggatatt cttatatata gaacatccgt
     1621 aagccaactt aaaactcctt aacaaaattt ttttactcat attgtagatc aaaacaagta
     1681 tccaagaaat catattttat ttcttttttc tgcttactca acaacagcta aaagaaaaat
     1741 catcataaac aaattcaact aagccaacga tccccttgtg aaatcaagtt ttttcttgat
     1801 ttacgaataa agagctaaaa taaaccaaac ttcttctttt ttacctcttc atagactgga
     1861 taactgattt catccccgtt gatcagatcc tttgcgactt gtttcatctg tactactttt
     1921 tccttaccaa agtcctttgc aatctgctca tagcactctt ttaagtaaaa ggtgcgctta
     1981 gagaccccat gggcatcaga agcagccatc tgcacgagat tatgtttgac catcgttttg
     2041 gctgttttct ggatgtcttt gccaaatacc ccaacataac ttggcgcagt gagttgggcc
     2101 aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttcgcgaaa atgcacgttg
     2161 cgttctggat ggacgattac tggtgtcttt cctagttcta acaactcaaa aaaaagccgt
     2221 tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt atcatctaaa
     2281 tctgtaaata atatttcatc tcggtggatg tcttcgatga gcgttccggt aatacgtact
     2341 tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc ttgtaacgaa
     2401 gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt gtggtgtggt
     2461 gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc aatacttgct
     2521 tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag atcgatcatt
     2581 tctcttgtct cctctcttct tattccttaa tttccgtagt aataaccagc atctttgcta
     2641 tgttctgctc cgttgtacac aacacctaag actcgtgctt gtaccatctc cagcagttct
     2701 tttgctttag tcaaggattc tttacgtgac atattttcac gtacgaccaa taaagtgcca
     2761 tccgttttta aagacatgat ctgtgcatct gttacggcta ctactggtgg catatcgaat
     2821 atcaccacgt caaataactg tcttgcttcg gataggatct gatccattct tggcgagctt
     2881 aacaattctg atgggttggg tggcttaggt ccactaggca aaatcgacaa gttctcgact
     2941 ggcgttcgtt ggatcacatc tgctacactt cctgatgaac taagtgctgt acttaatcca
     3001 ctggcattgt tcagctggaa tgtcttatag acgacgggtt tacgtaaatc tgcatcgact
     3061 agtagaactc gttgacctga tttagcaaaa acgactgcaa tgtttgctgc agtggtggat
     3121 ttcccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg accagctgcg
     3181 gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg tgaagatggg
     3241 tttgttaatg tgatcaaact gactgcatga gtttgtgctg ctttttgttt ctgtgttcgt
     3301 gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggggct gttttatcgt
     3361 ttgtagcttt tggtcgtgca ggagctgttc cttttttacg gataccagcg ttcaattctt
     3421 tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatct gtcacatatt
     3481 tttcatcttt cactgtccga tcgagtaatt ccaataagaa tgccagacct acacctacca
     3541 tcattcctaa cattaagcca gtcattaaat tcaatttatt atttggcgaa acaggtgtcg
     3601 tattggcaac tgcacctgaa atgatcgaga ttttatcaac attcatcaca tcttttgcgt
     3661 tctcttggaa aacttgtgaa gttgtgttag cgatctgttg agcagccact gcattcgtac
     3721 ttattgcctg aatcgagaac atttgtgaat tttctgcctg attaactgag atggcatctt
     3781 tgatttgttc agcagtcatt ttcacattgt cttctgtttc taaacgatct ttgacttcat
     3841 tgagcactaa atcactgacg atcatatctt tatacgtatt gatcatttgc aggttcgtat
     3901 tgacatcatt gacatttgca gtgtctgatt gtggcagtgt cacgatcaat tgtgcttgtg
     3961 aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc aagccagcaa
     4021 acattgagat gatgattatt accaagtgtt tgcgtaatgt ttcaaaaatt tctttcaagc
     4081 tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc atttgatctt
     4141 ttaattcttt tttcacacga tcaagttctt gatcactgac tttttgatag gagatattat
     4201 cttgcatgaa gccttctcct tgcagctgat cttgcttaat cgtaccaaat gcctcccgat
     4261 aatctaaagc aatttttttc atttcatcaa agttcatgtc tgttttcata ttcgactcta
     4321 gagcattcaa aacggattga tagttgctga caccgtttag actcaatact tttttcgcta
     4381 cgccttctac gatctttctt tggcgccctt gtctaccgta atcaccattt gggtcttcat
     4441 aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtaatttta ccgattggaa
     4501 agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca ccaactgcat
     4561 caacgagttc ctttagtcct ttcatattga tagagacata gtgatcgatt gggatgtcta
     4621 catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga
     4681 tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga atgctgacga
     4741 tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct gatcttcctt
     4801 gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag aaagattctt
     4861 gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact gattcgtaag
     4921 ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct acaattgcta
     4981 atactaatac gataagaact gctaaaataa tcaaaaatat ttttttccct cttgattttc
     5041 ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcaa cgttttaact
     5101 gtcaatagct tatattgcta tagcccattt gctgagcaac agtttgtgct tttgttgcgt
     5161 atgcggctct tattttttca ttccggatct gctctatttg actgagcgcc gtcaaatatt
     5221 gatcgtaagt agcggtagct gttacttgac cgtctttcag catcgtatcg aatagctgat
     5281 ctagttgcgt gacttgtgca atttgggtat cagcgaatcc tagatattgc ataacgactg
     5341 ttttccaagc actatcctta aaaaagctca tattttcacg tacatgggcg acatctgcac
     5401 ttgccaactt ttctttgatg atgacatcat ccacagcttg ctgccagttt gatacgtttt
     5461 tagtaaataa tttattgaca gccgtctgga tcttcagttt tgattctgca tcctctaact
     5521 gcgttaagac tgctttcttt tcttcttgga tcgcagcagc ttctttaggt aattctgatt
     5581 ccttgatgtc ataatcatct gccgaaagtt tgatagaaga tagtttatgt gacaaatttg
     5641 aaactgtttc ttctgttatt tcaggattta ggaaaatttt ttcttttgta ctgtacagct
     5701 tatctacttc ttttgataaa gaggtcacgg tgtctttttg attggtgatg gcagattctg
     5761 cgtaattgat ccgttgatga tattgccgct gattattgag atggtatcca aacaagcaaa
     5821 tcacaatagc gcctgtgcct accgccacag tagccaataa cttgacgaac ggcttttcca
     5881 tagattcact cctaaatatt gatgatagat aagtaaagat gtgattcatt cacatcagaa
     5941 tgagcgcttt ttattttaaa agcataatta aactaatata ttcatagaa
//
LOCUS       DAZKNM010000146         5874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000146 DAZKNM010000000
VERSION     DAZKNM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.148"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1292
                     /locus_tag="ACG8M9_002300"
     CDS             138..1292
                     /locus_tag="ACG8M9_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0379018.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1265..1708
                     /locus_tag="ACG8M9_002301"
     CDS             1265..1708
                     /locus_tag="ACG8M9_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379019.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2820..3788
                     /locus_tag="ACG8M9_002302"
     CDS             2820..3788
                     /locus_tag="ACG8M9_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0379020.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3798..4286
                     /locus_tag="ACG8M9_002303"
     CDS             3798..4286
                     /locus_tag="ACG8M9_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0379021.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4582..4863
                     /locus_tag="ACG8M9_002304"
     CDS             4582..4863
                     /locus_tag="ACG8M9_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379022.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4865..5281
                     /locus_tag="ACG8M9_002305"
     CDS             4865..5281
                     /locus_tag="ACG8M9_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379023.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2186 a         1014 c          960 g         1714 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatttaaca caaaggcgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc
      181 tcttttcctt gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa
      241 attctaattt caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa
      301 ctgattttga acactccata acttcaattc ttttaatgga aaataacacc gaaactgata
      361 ttaactatac tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt
      421 ctattttctt tccagtattt tcagacgctg ataatacgat taaatatgtt tataccgtca
      481 aaaaaaatac aaatggagaa tattcaggaa gtatttcaca atttttagca aaagaacttc
      541 aaaatctagt agatagtaat ataacagata caaccttatt ttttcaagat aatagcattt
      601 attattccga taatggacaa attaaactac tttggaaatc acctttaagt acagaaaaaa
      661 atggtgtttc ttcccttaca gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa
      721 ttaaaaattt ttctaaaaaa caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct
      781 ccggagataa ctttattatg attaattggc gtatagcaga aacccaaaca agttttccgt
      841 ggtgcgctgc ttacgtagaa gctgctatct tatgtaacaa aacagatgta cgtgctactt
      901 cagctagagc cattatgcat tatacctatc ctaatttaaa caacagtcaa attgaatcaa
      961 aaagtcttaa tcaagatcaa ataataaaat atgcaaatta ctgtggtgtt tatcctaaaa
     1021 aaatagcacg aatactaaac ctttcagagg tccaaacaca actaagaaaa ggaaatgcca
     1081 tttatatggg aactgctggt tatggaaatt atagtaacag ccgtcatgca cttgcattat
     1141 ttgggtgggt caatcaaggt ggttatcaaa cttactatgt gtggaatcct tggtataact
     1201 acccaactgt aataagtgca aatgcaggac cagttacaat tcctgtacct ggaggtggtt
     1261 ttacttggga caataccatt acaaattggt aaataaactc attgctttag ttatgggtct
     1321 agtgttagtt cttttcttaa ctagttgttc aaaaaaacaa gaaatacaaa agactgatct
     1381 gattcctttt ctaggaactt ggcaaactaa agatcattca gaacgtctag taatcactaa
     1441 tttaaatact tcaaaaaata tagaacaata taccatctta gtaaaagaga acaatcataa
     1501 aattcaaatc attgataaaa atttaaatat aaatgaacaa gttttcattt acaaagcaac
     1561 aaataattta ctaacactgc gctcgaataa aaaacaatta gattacactt ttagtttgga
     1621 ttctccaaaa aagctcgtat ttattttgac aacgaatgat gggacagaag ggacagctaa
     1681 accaattgaa ttttttaaag ctgattgatg aatagttgtt tgatattaca aaacaataat
     1741 ttcaccgaag gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagcctttt
     1801 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1861 agcggttatt tgcaccaaat acgtaggtat ttggctagca aatatagagc ttgccaagcc
     1921 ctttagtgtg ttttgtagaa agggacctta tagtcacttt cacattacaa acgatacgtc
     1981 ctatggacgt atcaaaaggg tgggacttat gagcaaatta aagcaaaatt tggctagcaa
     2041 tccttctaaa aaatacacct attgatcaga gtcaaaataa atcagttttc gagtgagcga
     2101 atccaaattt gcaactctga agctttgcaa atgagtgtgc cggcttccgt aaaagtctag
     2161 gtaacattag tcgatatata catagggctc taatttgcgt tttaaggcgt tttaaacgtt
     2221 ctggtatgta attataccaa agcgacttta aaacgcctta aaacgaattt tagaggttct
     2281 gtagaacgtt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaaaaaca
     2341 agataaggga ttttatctag acttgtctta aaacgccgta gatgagcgct cagagcttct
     2401 aaaaggactc cttacgaact gtttttaagc aaaggagcct ttaggcgact gcgcagttta
     2461 atgtgagagc gtagcgatca cacctttaaa gcgaccgtag ggagcagatt cccagtttag
     2521 gaaatttcgc gaacctgttt ggagaaaaaa agtaggaaaa ttttaggcgg gaaattttgc
     2581 ccccctcttt tataatctct tttataacct ctttttaaac ctctttaccg tcggctgtat
     2641 ccttactctc ccaaggtgtg cagaatcttt taggggtctt tttgtctgtt tttaggggtc
     2701 tttttgtctg tttttagggg tctttttgtc tgtttttagg ggtctttaat tagggggttg
     2761 acccccatta gaaagctctt tataattttg ttaagtagtt aatcttgaag gaggatttta
     2821 tggcaaatga aattgtgaaa taccaccatg aattaaatac gattccttta cgcaaattta
     2881 cgtcagtaga aatgaattta ttcttctcta taatatctag aatgcgtgat gtaggcgata
     2941 aaaaagtaca atttacattc gaacaattaa aagatttaag caattataaa gcaactgcaa
     3001 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     3061 gtagaagaag tgctgacggt ttgcaacgtg aacgatttgt tttatttaat caatttaaaa
     3121 tcgatggaaa agctgatatt ccttttgctg aaatacaagt ccatgagaaa gccttaccct
     3181 tacttaataa tcttgaagaa tgggttcgat attctctcca acaattcaat gaattagaaa
     3241 gcagttattc caaaaccatg tttagattat taaaacaata cagaaccaaa ggaattgcca
     3301 atttttcaaa agaagacttt catgaattac ttgatatccc taaaagttat agagaaagtg
     3361 atataaataa aaaaattata aagcctattc gccaagaact aaccgcaatc ttcaaaggtt
     3421 tgacaatcca aaagagatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     3481 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgctcca accaaagaag
     3541 aacaaaataa agctttggaa ctagctaatc aacaggttca atctgaaaaa gaacctactt
     3601 cattacaaga aaaaatcaaa gaacaaaaac gaaaattaga acttttacaa cagttagcag
     3661 aattagaaag aaaacaaaat accccaagta agaaaattaa caaacaaata gcagataaca
     3721 attccgatat taccccagaa tttaaagaaa acttactaga taatctccaa aacttattta
     3781 aagaataagg ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg
     3841 tttcaaaaga taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa
     3901 aaggaaatat tacctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag
     3961 gtaatgaatc gggtaactta cctagtgaat tacccgattc attacctacc gattacctac
     4021 tcaaggagat tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac
     4081 ttcaaatagc tttagaaacg cagcaaaaat tactcgacca gcaacaacaa ctcaccctac
     4141 aagccaatca acaaatcaaa aaacttcacg aacaactcca gctaacctat gcagaagaat
     4201 cttcagaaaa agaacccctt tctgttactg aagaaactca aaaaggacaa aacaaggaga
     4261 aaaaatggtg gcaattctgg caataaccca acaattttta ttcataaaaa ttgttgggtt
     4321 actttctttt tttgctttta caagcatagc gacatgatct cacgtcgcta ttttttaaag
     4381 agcataagga aacgcggtat cactttctta cgaaaggtgc taggtaggct gtgcctacca
     4441 cgtttctaag aaaaaaattt gctatcactt tttcgtcatt gtggatagct tatgtatagt
     4501 gtttcggcaa aaaacagcta tacaaatact atatttgtat gatatacata aattatacac
     4561 aaatgcgaaa ggaattcttc tatggacatt ttagttcgga atattgatcc aaaagttatt
     4621 tcagaaatag atagacgttg ccaagaactc acaacaaaaa ctggcaaaaa atggagccgt
     4681 aatgcgtacc taaaagttct aatcgaaaac gattttgacc aggcacttag gcattacaaa
     4741 aaagatcaat tcgatttatt actcgaaaaa tttatcgata tacaagcaaa aaatactgaa
     4801 acattgaacg actatgttca tacaaacaac caactcattg aaacacttat cggaggaata
     4861 taacatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acattgacgt
     4921 tttagattcc atcatcgaaa aaaatccaga tatcaataac tactcggaag ccattcgttt
     4981 tctatgtatg agttatgaac ctaatactaa tacgacaact accagcaacc aagcaaaact
     5041 aaatgcgatg ggaaaagaaa tttctattat gtccgaaatc attgcagaat ttgctgattt
     5101 atatctaaaa gatactggtg ttcttatcgg tacaaattct aaagtatatc aagaagtgaa
     5161 agaaaaaatt gaaaatcaac tcaatccctc ccgacgaaat caacccaaaa atcgtcaaaa
     5221 agaaaccaat caaacattca cttctagaaa ttttgaccca acaaactttc taaaaagatg
     5281 aaaaagaaac cgaaaatttt ttctatttct cagtttcttt tttagggggc agtcttccag
     5341 caagatttgc aagttaccgg taacttgcac aaaaaaagaa aaatccgctt gtgcgaatcc
     5401 ttctttttgc attttggggc gctgccccaa tctccaaata tcggttattt aatagcgtgg
     5461 taactcaaaa aaatagaacg tactctgaaa taaaaaaaga ctgctctaac gagcagccaa
     5521 taaaaaataa tgagcgttta ttcttgaaaa agcagcgcgc ttatgaagcg ctgccaaaaa
     5581 tttaccatat tttatgaaaa ataacaagtt tctaaaaagg aatcggttaa gattcacaaa
     5641 gaatcgtaaa cagacaatag cggaaaatca agcttccgaa cgtacctttt agctagacca
     5701 atgaaaaatc acaaagtgag cccctgtatc ggttgataca agggctactt ggcatactcg
     5761 acattatgtc tactagctcg ataaataacg attatgttcc caaaacaata ttttatgaac
     5821 tataacaaaa atttgttatc gcatattgta tattttaatt ataggtgtat atca
//
LOCUS       DAZKNM010000147         5855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000147 DAZKNM010000000
VERSION     DAZKNM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.149"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..685)
                     /locus_tag="ACG8M9_002306"
     CDS             complement(38..685)
                     /locus_tag="ACG8M9_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0379024.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(693..1379)
                     /locus_tag="ACG8M9_002307"
     CDS             complement(693..1379)
                     /locus_tag="ACG8M9_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379025.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1385..2458)
                     /locus_tag="ACG8M9_002308"
     CDS             complement(1385..2458)
                     /locus_tag="ACG8M9_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0379026.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2680..3354
                     /locus_tag="ACG8M9_002309"
     CDS             2680..3354
                     /locus_tag="ACG8M9_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0379027.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3359..4699
                     /locus_tag="ACG8M9_002310"
     CDS             3359..4699
                     /locus_tag="ACG8M9_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0379028.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4848..5834)
                     /locus_tag="ACG8M9_002311"
     CDS             complement(4848..5834)
                     /locus_tag="ACG8M9_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0379029.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1768 a         1123 c         1034 g         1930 t
ORIGIN      
        1 taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct tcagcaatcc
       61 aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta tttgcaatga
      121 gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta actgcttctt
      181 ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt tcgttcattg
      241 gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca
      301 agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac tctaaatatt
      361 tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag atatcttgat
      421 ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa tcttgaaaat
      481 atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag actttgactt
      541 ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc tgtttctttg
      601 cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta tctctgatta
      661 tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc ttcttctgtc
      721 aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg gacatcatga
      781 gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa tttgacgact
      841 tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat caagcttggc
      901 tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga aaggtcttta
      961 ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc gatttttgtt
     1021 gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt taccgttaaa
     1081 aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg taaatctgct
     1141 cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc tccggaagtc
     1201 ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc agaaggacca
     1261 atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa agctgagacc
     1321 tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat ttttttcatt
     1381 tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca
     1441 atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa
     1501 aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag
     1561 caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga
     1621 agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca
     1681 tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg
     1741 catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag
     1801 ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta
     1861 cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag
     1921 cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa
     1981 agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt
     2041 tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat
     2101 gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat
     2161 tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc
     2221 cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat
     2281 cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg
     2341 aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac
     2401 cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt
     2461 gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact
     2521 ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat
     2581 ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc
     2641 catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta
     2701 gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata
     2761 acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt
     2821 gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg
     2881 aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag
     2941 attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa
     3001 ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt
     3061 ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa
     3121 gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga
     3181 atcattttga caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg
     3241 attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg
     3301 gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat
     3361 gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt
     3421 tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata
     3481 cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga
     3541 gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag
     3601 aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat
     3661 cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca
     3721 aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa
     3781 tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa
     3841 tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt
     3901 agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg
     3961 tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca
     4021 aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa
     4081 tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat
     4141 cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat
     4201 cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata
     4261 tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat
     4321 tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt
     4381 ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga
     4441 ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag
     4501 tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa
     4561 agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca
     4621 tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc
     4681 gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac
     4741 agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga
     4801 aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta
     4861 ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca
     4921 tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga
     4981 ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca
     5041 tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg
     5101 acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg
     5161 tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt
     5221 agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta
     5281 cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat
     5341 ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg
     5401 ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt
     5461 gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca
     5521 gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca
     5581 taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca
     5641 tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta
     5701 gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc
     5761 aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa
     5821 tttttctctt ccatgtaaat tcctcctagc aaata
//
LOCUS       DAZKNM010000148         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000148 DAZKNM010000000
VERSION     DAZKNM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.151"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8M9_002312"
     CDS             complement(488..1297)
                     /locus_tag="ACG8M9_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0379030.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8M9_002313"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8M9_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0379031.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8M9_002314"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8M9_002314"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0379032.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8M9_002315"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8M9_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0379033.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8M9_002316"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8M9_002316"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0379034.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8M9_002317"
     CDS             4179..4913
                     /locus_tag="ACG8M9_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0379035.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8M9_002318"
     CDS             4897..5607
                     /locus_tag="ACG8M9_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0379036.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKNM010000149         5555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000149 DAZKNM010000000
VERSION     DAZKNM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.152"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..729
                     /locus_tag="ACG8M9_002319"
     CDS             28..729
                     /locus_tag="ACG8M9_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0379037.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            726..1847
                     /locus_tag="ACG8M9_002320"
     CDS             726..1847
                     /locus_tag="ACG8M9_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0379038.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1862..3094
                     /gene="pepT"
                     /locus_tag="ACG8M9_002321"
     CDS             1862..3094
                     /gene="pepT"
                     /locus_tag="ACG8M9_002321"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0379039.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3226..4134
                     /locus_tag="ACG8M9_002322"
     CDS             3226..4134
                     /locus_tag="ACG8M9_002322"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0379040.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4169..4441
                     /locus_tag="ACG8M9_002323"
     CDS             4169..4441
                     /locus_tag="ACG8M9_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0379041.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4452..5498
                     /locus_tag="ACG8M9_002324"
     CDS             4452..5498
                     /locus_tag="ACG8M9_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0379042.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1810 a          966 c         1211 g         1568 t
ORIGIN      
        1 ctataattat ctagaaggag tgaagctatg aaccatttag atttatctca gcgcttagcc
       61 gcagtgggtg actttgtccc tgaatccgct cgtttagcag acattggatc agatcacgct
      121 tacttacccg ttgcattgat attaagagga aaaatcgaat atgcagttgc tggtgaagtc
      181 gttcaaggtc cttatgaatc agcacgtaag caagtcaaaa aagatggatt agaagaccgt
      241 atcaccgtac gccttgcaaa tggtctagat gctatccagc cacaagatgg tattacagct
      301 gtcacgattg cgggtatggg aggttcactg atccgagata tcctagaagc tggcaagttg
      361 aatcaacgct taagcggaca agaacgtttg atccttcagc caaacatcgg agagcgtaca
      421 ttacgatctt ggctgcaaga aaatcagtat aaaatccaat cagaaaaaat acttgaagaa
      481 aataaaaaaa tatatgaaat cattgtagcc gaaaaagaac aaaaaccagt tacttacaat
      541 gaaatggagc tgatgttcgg gccatttctg cttgaagaga aaagtgagat cttcaaatca
      601 aaatggacaa gagagcttca acaaagaaaa gctatccgtg aacagctcaa acaagcagct
      661 aatcaagagg cacgtcaaaa acaaatcgag caggaaatcc aaatgattga ggaggtttta
      721 aagccatgac aatcagcgga cgcactttca tccaaaagtt tgaaagctat tgtcctcagt
      781 ggctagctga agaaggagac ccagtcggac tgcatatcgg aacattggat aagccaatcc
      841 aacgagtaat gatgacatta gacgttcgtc cagaagtggt cgaagaagca atcaagaaaa
      901 aaatcgattt gctgattgct aaacatcctc caatctttcg tcctgtaaaa cggctggtta
      961 ctgatcagcc acaagaaaaa atgtatgcag atttgttgaa acatgatatt gccgtctatg
     1021 cagcacacac gaacatggat attatttggg atggactgaa tgactggttt tgtgaacttc
     1081 tcggtattga agtggagagt tatttagtaa agactcatga gattcattat aaaaaactgg
     1141 ccgtttatgt acctgtggat catgcacaga aaatgcgtga agtattagct gcagctggtg
     1201 ctggtacaca aggtgattat accggtacaa gttttacctc gattggacac ggtcgattta
     1261 ctccggaagc tggtgcacaa ccagcgattg ggaaagtagg gaagacagaa caagttcaag
     1321 aggcaaaagt tgaggttatt ttgccggaaa ctatcgaaaa acaagtgatt caagccatgc
     1381 ggtcagctca tccttatgag gaacctgctt atgatctttt cgctatagat gaaccagtcg
     1441 aaatgtttgg gttaggacga gtaggtgaat tgccgcaaga aatttctata gaggcatttg
     1501 tagaacaagt aaaagaagcg ttccaactag atggactgag aatagtccag ccaaaaaatg
     1561 caaaatcctc tgtgaagcgc attgcgattt gcggtggaag cggtgaaaaa ttttatccgc
     1621 aagcaatcgc ccaaagagca gatgtctata tcacgggaga tatctattat catacggctc
     1681 atgatatgca aagcgctggg ttgattgcaa ttgatcccgg tcattatatt gaatcgttat
     1741 gcaagcaacg atttatcgag aaatttgaat cttggaaaca agaagaaaat tgggatatta
     1801 actttttcgt ttctgaaaca aatacaaacc catttcaatt taattaaaag gagagaatac
     1861 tatgtacgaa aatcttttgc cacgtttttt acgatatgtc aaaacagaaa cacgctccga
     1921 tgcaacaagc aaaacgactc catcgactca gacacaagtt gcttttgcgc aagaattgaa
     1981 aaaagaatta gaagagttag gcttagctga tgttttctat aatgaagata acggattcgt
     2041 tatgggaaca ttaccaagca atgttgatca tgaagtgcgt tccattggat ttatttccca
     2101 catggataca gcagatttta atgcagtagg agtcaatcca caaatcattg aaaactatga
     2161 tggcgagtct acgatcgtac tagatgcgga agaacgctat acattgaata caaaagattt
     2221 tccaaatttg aaaaattatg aaggccacac attgatcaca acagatggca gtacattact
     2281 gggagcagat gacaaatcag ggattgcgga aatcatgact gcaatggaga tcttgatcaa
     2341 taatccttct atccctcacg gtaccatcaa agtggctttt ggtcctgatg aagaaatcgg
     2401 tgtcggagcg gataaattcg atgttgagca attcgatgta gattttgctt atacagtcga
     2461 tggtggtcct ttaggtgagt tgcagtatga aacgtttagt gctgctcaag caaatattac
     2521 gatccaaggg aaaaatgtac atccagggac ggcaaaagat acaatgatca atgctctaca
     2581 gttagcaatc gattttcata atcagcttcc agccgatgaa gttccagaaa aaacggaagg
     2641 atacgaaggt ttcttccatt tgatggcatt ggatgggaat cctgaagaag cttctatgag
     2701 ttatatcatt cgcgatcatg atcgtgaaaa attcgaagca cgcaaagcac aaatcacaca
     2761 tatccaagaa gagttgaatc agcgatttga tcaagaacga atcaaagtag atatgtatga
     2821 tcaatattac aacatgaaag aaatcattga aaaagatatg agcatcgtgg aattagcgaa
     2881 acaagcaatg atcgagcttg gtatctctcc agtgatcgaa ccagttcgtg gaggaacaga
     2941 tggttcaaaa atctcttaca tgggtattcc gacgccaaat ctatttgccg gaggagaaaa
     3001 tatgcacgga cgattcgaat ttgtttcttt acaagtaatg gaaaaagcaa cagacgtgat
     3061 tgtaaaaatt gcagaattga acacaaagag ttaaaatcga aaaagaacag cttatgacaa
     3121 ggcgttcgtc ccaatgatgg aatgtcatct gtctaaggct aaagggcagg acaatggaat
     3181 tggttgtccc gccctctttt ttgaagagta aataggaggt aaagaatgga aaagctgcta
     3241 gttttttata acaaaagttc agggaaagat gagggtgaga aattagcacg ctggtttcaa
     3301 gattatgtaa aagaaaagca gccagaatta tatgtagaac tagctgaaac tggcccatcg
     3361 attgatgatg cggaattagt caagaaagca gaagccatag aagctgatac tttagtaatt
     3421 ataggtggag acggcacgat tcatcatatc gtccaagcgt tccaaaaaac attatctcaa
     3481 taccatgtcg gattacttcc aggtggaact gtcaacaacc ttgcacgtgt tcttgagatt
     3541 cccctcaaaa aagaagaagc tgctgacgtg atcctggaag gcaatgtacg gaaaatagac
     3601 tatggtcaag tcaatgatga agtcattatc tctacactga caatcggaat cttagcagat
     3661 acagcagttt ggatcagtca agaagaaaaa cagcgttacg gctcgtggat ctttatcaag
     3721 aagtttttta agttattgct gaagaagaaa aaatatcatt tggatttaga aacagacgag
     3781 gagcattgga aaggaaaatc ccaactgttg acagtcacga tgtctaactc cgtgggcggt
     3841 tttacaaatt ttgacgactc agcgacccta gatgatggaa aatttcatgt aacgatcgta
     3901 ccttccttag atattttccg atttattttt tatttgccta gaatcatgag aggcaaattc
     3961 tatacgatcc ccggcattac ttatttcaca gccaaacgca tgaaaatcca agcgcaggaa
     4021 aaatctgtgg gcactcgaac ggatggagac acgacagatg atcttccagt tgaattatct
     4081 gttgtatcaa aaggattaac agtttattca ccaaagaaag aagaaaaagc ttagaaatag
     4141 tagttaacag aaagttggaa gaagagtaat ggatgtattg ttacacaatc ctttgtggtt
     4201 ttaccttttc attgtgaatt tttacttatt ttgtctgatg gggtatgata aacatcaggc
     4261 tagaactagc ggctggcgtg ttcctgagaa taatttgata tttgtcagcc tgttaggcgg
     4321 tggtttggga ggattgatct ctcgaaaagt tttccatcat aaaactcgga aaaagaagtt
     4381 tactatagga ttcgctgcag gtgtacttgt tgatttgatt ctcatttatt ttttccatta
     4441 aggagcagga gatgtcgtct aaaggaacga aaactaaaat cattctgaat ctgcttttgc
     4501 tgattgtgat ttttgccatt atttattatt taatcaatca atcatttgca gatatctttg
     4561 ccgaattgct gtctaccagt gttgttgttt tggcagcggc tgttatatta gggacagttt
     4621 accaaatcgc tgaagggcgc tcaatcaaag aaattgctga acctttcgcg ccgaatttta
     4681 caacgaaaga tgggttcttg acctctagct atattgcgtt ttaccgaatc gtttcgtttg
     4741 gtacaggaac gctgatctcg gagatttatt tttataacaa aaaaggaatg aagctatccc
     4801 agggagcagg ggtgacagca ctccatatga ttatgtacaa actggcagtg atcacttatg
     4861 cattagctgg cttggtcatc caattctctc ttttttattc taatggtcca tcaatgatct
     4921 ggtttatcat cgctggtatc cttttgacga tcgtgattgt tgccttttta ttggcgatgt
     4981 ccgtcagctt gaatgttcaa atattttttg tcagcttatc gaataagtta tttaaaagtg
     5041 agcgtttgcg gaaatgggtg gatacatgca atcagcagat ctactcttta agggaaacgg
     5101 tccaaaccat tatccaagac aggactgcgc tattgcggat ttatctatgg aatatgttaa
     5161 aacttgtatt ttggtatgta cttccttatt tgattctgat tgaaaaccat cctaaaatag
     5221 attttctact gaccatttcg tttatcagct ttgcagtagt gctttcaggg gttattccta
     5281 cgcctgctgg tattggttcc tttgaatttg tctatttgtt attattcaga ccagttgttg
     5341 gaacagttga cgcagtttct tcgcttttac tgtatcgttt cagttccttt atcttgccat
     5401 ttctctatgg atttgtgtat gtgattgctg aacggcgaag agtgatcaaa gaagaaataa
     5461 tagaagtaaa aaaagagaat aaagaaaatc aagggtaggt cttttataag atctaccctt
     5521 tttatcaaat caaagaaaaa cgttatggat aagat
//
LOCUS       DAZKNM010000150         5545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000150 DAZKNM010000000
VERSION     DAZKNM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.153"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            239..577
                     /locus_tag="ACG8M9_002325"
     CDS             239..577
                     /locus_tag="ACG8M9_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379043.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(782..1344)
                     /locus_tag="ACG8M9_002326"
                     /pseudo
     CDS             complement(782..1344)
                     /locus_tag="ACG8M9_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2076..2657)
                     /locus_tag="ACG8M9_002327"
     CDS             complement(2076..2657)
                     /locus_tag="ACG8M9_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0379044.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2840..3466)
                     /locus_tag="ACG8M9_002328"
     CDS             complement(2840..3466)
                     /locus_tag="ACG8M9_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0379045.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3488..4819)
                     /locus_tag="ACG8M9_002329"
     CDS             complement(3488..4819)
                     /locus_tag="ACG8M9_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0379046.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4974..5375)
                     /locus_tag="ACG8M9_002330"
     CDS             complement(4974..5375)
                     /locus_tag="ACG8M9_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0379047.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
BASE COUNT         1572 a         1188 c          910 g         1875 t
ORIGIN      
        1 aaaattcccc tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat
       61 ataaatagaa ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga
      121 aacattgaac ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt
      181 caattttgca gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat
      241 gcccgattat aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa
      301 gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca
      361 aatagaaaca ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga
      421 gatcgtagct atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga
      481 taaatggcaa cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac
      541 tttgaatttt agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa
      601 taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac
      661 tgtgacccta tagatgcagt atatgcagct tatgagcatt tcttctgttc tctttgtttt
      721 tgggatctgt gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat
      781 gttatacctt ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag
      841 aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat
      901 ggattggtgc gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt
      961 catcctgatg gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca
     1021 taaatttgtc tttttcttca ttccactcat tgggaaaaca caacgatttt gcaaaaatgg
     1081 ttgatgaaag gtcttttttt cttctactgt ctctgcacgg gcattaatca tcaattgtcc
     1141 ttttttaaat ccggtaaatc cccacttcat gatttccgga gtgaggatgt tttcttcctc
     1201 ttttcttaac gtcacaacaa gatttgacgg aaaaacttct ccagctgcta attctgctgc
     1261 ctgcttcggt tgtttcgtct gggcttctcg ataataattc tgaaggagct ctgactctag
     1321 atcaaaaaaa taacgaccac acatcctaat ccctcccaat ctttttttct atcatacact
     1381 gcaacaagaa gttcgcacca acaaaacgct ttcaattttt gtagaactaa aaaagttgtg
     1441 acaagtgaca aactctccag tgaatggtat tctcctgcca actcctcggt cacaagtcac
     1501 aatattcaac aaacatgaga agctgctttt tgaaattgtt ccttgtgact cggagtgaaa
     1561 cggcaggctt acgacctctg ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc
     1621 aaatttatgc aaaaatgtag aaagctattt tcacaaaatt tttcttaata ctcgaaacta
     1681 agcgactttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata
     1741 agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat
     1801 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagcta accttcggta
     1861 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
     1921 ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa
     1981 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
     2041 ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac
     2101 aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc
     2161 aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct
     2221 tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat
     2281 tgattgacta attctagtag caacagacgg tattcttcat ccactgtact caactcaata
     2341 atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc
     2401 cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg
     2461 aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt
     2521 tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct
     2581 cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc
     2641 ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta tattatacag
     2701 taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac
     2761 agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag
     2821 gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt
     2881 gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac
     2941 caattgcatc tgtcgctacc acaacattga atccactctc taccaattca cgcatatggg
     3001 aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc
     3061 tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga
     3121 tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt
     3181 ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc
     3241 ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga
     3301 gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt
     3361 atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga
     3421 cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat
     3481 tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt
     3541 gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga
     3601 cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga
     3661 ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg
     3721 tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac
     3781 ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg
     3841 ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga
     3901 tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg
     3961 tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt
     4021 ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt
     4081 ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg
     4141 ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta
     4201 aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa
     4261 cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag
     4321 ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa
     4381 tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat
     4441 tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt
     4501 gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga
     4561 tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct
     4621 ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga atacagccga
     4681 tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac
     4741 cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat
     4801 tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa
     4861 tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca
     4921 gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca
     4981 atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc
     5041 gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat
     5101 actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact
     5161 gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa
     5221 gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga
     5281 ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca
     5341 tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat
     5401 ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc
     5461 ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact
     5521 cgtgaacaac agcgtcatat ttgtt
//
LOCUS       DAZKNM010000151         5517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000151 DAZKNM010000000
VERSION     DAZKNM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.154"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1530)
                     /locus_tag="ACG8M9_002331"
     CDS             complement(70..1530)
                     /locus_tag="ACG8M9_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379048.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(1738..2664)
                     /gene="glsA"
                     /locus_tag="ACG8M9_002332"
     CDS             complement(1738..2664)
                     /gene="glsA"
                     /locus_tag="ACG8M9_002332"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0379049.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2687..4114)
                     /locus_tag="ACG8M9_002333"
     CDS             complement(2687..4114)
                     /locus_tag="ACG8M9_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0379050.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(4145..5359)
                     /locus_tag="ACG8M9_002334"
     CDS             complement(4145..5359)
                     /locus_tag="ACG8M9_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0379051.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1730 a         1144 c          933 g         1710 t
ORIGIN      
        1 aggactcaaa taaaaacctt ctacagacaa ttaaaaagct ttgtagaagg tctcctaatt
       61 aaggtctcat caagaaatat gtttttttaa taggtgaagt tcctgagcaa tatgatacaa
      121 atggatgttt gcacctttta atgagattgg atagatcttt gtcttttcag tttctaatcc
      181 attttgatag ctagcagcat cgcacaaaac cactcttttt ttcgcacata gacattcatc
      241 aggcaattct tctaatgaac gaaataaaat aggatttatt tcagcgatgc cttcttccgc
      301 atacaaatga aattgggttc gataaaaaaa ttgtttgact gaactttctg aaacaacaaa
      361 aatttctatt tcctgtctcc ccgcttgttc actgttgatc aaagaaagac ttttgacgaa
      421 ttttcgaatg aaattttcat tccatcttag ttggatttga tttttctttt tccactcttc
      481 caatatttct acaatttttc cgtacagaag ttgttctttt tctgatagaa gatagatcct
      541 ttccggtaaa aatacttgtc catctgccca caacgtacgg acaaattgta aaaaggattg
      601 cttgaaaata aaattttctg catcgtcgat ttcagcgata ctaataatct gtaaaatcaa
      661 ttcattcagt gataggtcat tgagaacaaa atttttataa acaacttcga aatcttttct
      721 taacagctcc atgttctcat tggtataatt tctactgttg aacaaagaaa aaatataata
      781 gacatcttct tcttgtaagt ctagaattcc tttttgctct tccaatcctt gacagactaa
      841 ttgaaataac ggcttcgttt ctgcgctttt cttatcttct tctttcagtc caaggggaaa
      901 tgcctttctt ccgattgcga tctctattcc tcgtatgata aaggactttt catcttccga
      961 atatcttctt ttaaaaaaat gagtctctac ataatcaatc aactcgttac aagcttcttc
     1021 tacatacgga tttttatttt catagccttc ccaattgaca taacgtacta aagctagcaa
     1081 gatgttccgc gagtcaaatt ctgggataat gatttcttta tttttcaaat aatcattttc
     1141 tttaaaaaaa tccaatacga tttttttgat tgtatatact ttgctcaaag aaataaattc
     1201 aagatcagcc agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa
     1261 aaaataagat tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa
     1321 tacgagttta tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc
     1381 ggctagactt tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt
     1441 ttgttcgatg tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa
     1501 tatatttcgc tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg
     1561 attttattta atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct
     1621 ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc
     1681 tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta
     1741 ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc
     1801 gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac
     1861 agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa
     1921 ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact
     1981 ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc
     2041 agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc
     2101 gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga
     2161 gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt
     2221 aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc
     2281 tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg
     2341 atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc
     2401 cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac
     2461 gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag
     2521 ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa
     2581 tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa
     2641 ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat
     2701 gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag
     2761 aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa
     2821 caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt
     2881 ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca
     2941 gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg
     3001 acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc
     3061 agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt
     3121 cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag
     3181 gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa
     3241 ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa
     3301 tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc
     3361 caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct
     3421 ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta
     3481 aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt
     3541 tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa
     3601 acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag
     3661 tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg
     3721 ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac
     3781 cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga
     3841 ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt
     3901 tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata
     3961 gcaaaccgcc taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat
     4021 attcatataa tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt
     4081 tttttgcagg aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt
     4141 tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt
     4201 ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat
     4261 aggcatacac ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc
     4321 ttggattcgt tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga
     4381 atccgcccat gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac
     4441 gaaaatcaac agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt
     4501 ttgcttctac ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta
     4561 gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca
     4621 tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc
     4681 gaagtgcacc accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag
     4741 cagctgccat aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa
     4801 gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag
     4861 cgaaatctct aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag
     4921 ggaaataaat cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc
     4981 tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa
     5041 gaaagaacat taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat
     5101 gtctcatcaa aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg
     5161 ccaatccgaa tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt
     5221 gagacatgat cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac
     5281 caggagtcat caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat
     5341 tgataggtaa tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg
     5401 tgtagttgtt tttttcttct gtttttttga attaatactt gcatttataa aagacagaca
     5461 attaataaaa gcttgatgtg ttgtgctctt tttcgttttt cacacaaata aagacaa
//
LOCUS       DAZKNM010000152         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000152 DAZKNM010000000
VERSION     DAZKNM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.155"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8M9_002335"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8M9_002335"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG8M9_002336"
     CDS             complement(580..1353)
                     /locus_tag="ACG8M9_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0379052.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG8M9_002337"
     CDS             1497..1847
                     /locus_tag="ACG8M9_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0379053.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG8M9_002338"
     CDS             1828..2544
                     /locus_tag="ACG8M9_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0379054.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG8M9_002339"
     CDS             2544..3992
                     /locus_tag="ACG8M9_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0379055.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG8M9_002340"
     CDS             complement(4062..4571)
                     /locus_tag="ACG8M9_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379056.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG8M9_002341"
     CDS             complement(4561..5286)
                     /locus_tag="ACG8M9_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379057.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKNM010000153         5475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000153 DAZKNM010000000
VERSION     DAZKNM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.156"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..2091
                     /locus_tag="ACG8M9_002342"
     CDS             31..2091
                     /locus_tag="ACG8M9_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0379058.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2174..2743
                     /locus_tag="ACG8M9_002343"
     CDS             2174..2743
                     /locus_tag="ACG8M9_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0379059.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2918..3724)
                     /locus_tag="ACG8M9_002344"
     CDS             complement(2918..3724)
                     /locus_tag="ACG8M9_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379060.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3862..5376
                     /locus_tag="ACG8M9_002345"
     CDS             3862..5376
                     /locus_tag="ACG8M9_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0379061.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1945 a          949 c         1122 g         1459 t
ORIGIN      
        1 ttagacaaga gtagggagga ctaagaataa atgtgttcaa aaagacgttc aaaacaacta
       61 aataaacacg cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca ctacgttaag
      121 tttatgatgc gcacgatgct gatcagttta tctgtaggtg tctatatgca tgtacccgtt
      181 caggcaacga cacaaaccat caaggtcgta cgtacctctg tttctgagac cactgattcc
      241 cctatcacta ttactcatac tttcactgat gcaaatggaa aacagctact tcctgcattg
      301 acttcctata ataaagtcag ttatcctcca acgataaacc actactggtt aattcctaac
      361 aaaacattat ggacatcgga aaccggaagc agtgtgccgc tttctatctt ttccgatatg
      421 actgatccta caaaaggtga tcagttaaaa cagatcactg atattttgaa tcatgctttg
      481 acagatataa aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa agattattca
      541 cagtttgaaa gaaagaaaat cgatgttctg gcagggacaa aagtttcgat atctgatctg
      601 gtagaaaggc tgaagaacgc agatggaaca caaggagata tgtccaaagt aatttcaaac
      661 aatgggaata tcgtggatac gactaataca ggaacatcta ctatttcctt gtcctatttt
      721 gatccaattg ctttaaaaac aatgactgca tctgctgtga tccatgttcg tgctgatcaa
      781 acagctatca aaggaaaaga tcttcactta agaacaggag agagttacgc ccctaaagat
      841 cttgttgaat atgtaacaaa aagcgatgga acttcaggaa ataaagaaga aatcaaaatg
      901 aacgggacag tagatactac aactccggga gaatatcccg ttgtattaac ttatatagac
      961 tcgaaaacat caaatgtggc agaaacaaca gctgtagttt atgtagaagc atctgctgct
     1021 tcatcgactg tcatcactca ttttatagat agtcaaacaa ataaagaact tcaagcagaa
     1081 acacatatga gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga attttctgat
     1141 cgagtgccaa atatagattt atcgacttat gaacaaaatg ggaaagtcac ttctatttct
     1201 gattggatgc aagcaaacgg attagacaga aaaagtcact ggtcggagat ccttgaagta
     1261 cttcactcag aactgatgaa taataggaag caatcaggag gggaaattac ctttacttat
     1321 gtttatatac cagacaatcg ttcattagaa ggtcatgatc ttattgttca tccaaacgaa
     1381 aaaataaaca aagaagagtt gatccaatca gcaacaaatt ccttaggtaa acccgtaccg
     1441 ttaaaggatg tatcggttac aatcaatgaa gtaccattgg aggatggtta tttgaccaaa
     1501 caaacaggtg attttacgat tatgtatagt tatcatgacc cgtattcact ccaagaactc
     1561 catgctcagt cactattaca tgttttacca ttaaacaaac agatagatca aaaagaacaa
     1621 aaaagacaag aagaaagctt gtcagaaaaa aaacaacaaa tggctcatgc agtttctcca
     1681 acagggaaag taaaacaaac aagaagccta gagttagcaa gaatatctag aaaaatctca
     1741 gaatctaata aaacgataag gcaagaagct catttggccc cacaagtttc tggattggaa
     1801 aatgaaattt tagagcctaa aataaaacaa caatcagaaa aagaatttgt tgctaaacag
     1861 tcggaagatc agaaggaatc agatgaccag aaggaaacga agaaaaaacg cttgtcaact
     1921 attgattcca ccgatgagca tctgcagccg caagtaccac ttcctagtcc gggaacaccc
     1981 gcacctggtg gaggtattcc ttcttctgct ttaggagatt tcatgcgagg tatggcagga
     2041 acatgggtat acgctaatcg aaatgaattt gacgcagatg actggttctg atttatatct
     2101 ctatctgtta tagtcaaata agtagctgag tcatatgtta tttggcataa tggagacgag
     2161 gagggataga aaaatgttag ataaaacgtt accttacgca gaaatctgga tgacaagacc
     2221 acttcaacaa aatgttccaa tcacgccttt agcggaaggt tttgaatttc gattttatca
     2281 aaaagaagat gaatctgctt gggcggagat tgagacttcc gttggggaat ttcaaacgac
     2341 agatgaagca cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag aacttgaaag
     2401 aaggatgttt tttgtagaga caaaaaaagg agaaaaagta gcaacctgta ccgcttggcg
     2461 aaaaaaatca agagagcaag tgtatccggt ttttcattgg ttagcagtga aaccagatta
     2521 ccaaggaaaa gggctcgcaa aagcattgac tgccagagtc ttgcaagaat ttcctaccct
     2581 ccatacggac ggaccaatct atcttcacac acagacatgg agccatcagg cgattgctct
     2641 ttataaaaaa atgggtttta cttttattgc tgaaaatcta gacggcacac ctaatccaga
     2701 ttatgaaaaa gttatgcagg ttttaagtaa attagataaa taacgaaatg gtgaaataag
     2761 tcctcagtat tgggataaaa aacgaaatag tcattcctca tatcttactt ttttgagtta
     2821 atactatcgg ataactaatg gcatgccata cattcaaaaa agaaggggca gtgacaaaag
     2881 tattgtcact gccccttctc ttgtttttta gcatgtttta tcgtttgatt tttttaggaa
     2941 taaaattctt gatgaacgta aatttttggt aatcgtttcg actatagagg taaccaaata
     3001 cactgaaaac aactgctcca ataaaattaa caaataaatc cttcatagta tccatgattc
     3061 ccacgtctaa gtaaccattt tggatgattg ttttatctgt tttaccatct gcattttgac
     3121 tttcaatgat tgtccgagtg atatctggaa catgatagac agtgtttccg tctttactta
     3181 actccacact attgatttgc tggatgatcg tatctttttg catatcgaat tgcagtaatt
     3241 tatctgctga aaattccaca aactcccaca taacaccaac tgtcatcgaa aaacagaagg
     3301 aaacaactgc tagtaataaa ggagtcaagt tcatggtttt tgtattttta ttgagcaaat
     3361 aaacaaggga aaagccgaca cccccagcta aaaatccgct gaatgcatgt agaaaagtat
     3421 cccacattga aaaatagcca taaaaatcac ttaactctcc taaaactgtt gaactaaaaa
     3481 taaaagataa caaaatgatt tctagcatat ttggaatctg tatctttaat atcttttcca
     3541 gaaaataagg gatttcaaat aaaattaatg tcagtaataa aagcattgta tttccccagt
     3601 gttggttcca gacctgatcc actagcataa taacgacgat gatacgtaat aaaatataga
     3661 ttccgtttgt gattctacct gcagtttttg tactgttcgt aaaaaaggtt ttcaaatgat
     3721 tcataaaata actccttcat gtatatcctt aattatacta agaagcgtgt gtttaccaaa
     3781 gctatatgac ttaaagaaaa acagagttga tattgtgaaa aaaatcactt aatatagtat
     3841 aaggagaaaa gggggataca aatgaaaaaa ttagttgctg gtttattaac agccgttgca
     3901 cttcttgcta tgagcgcatg cggcgcagat cagaaaaata cagcgcagac aagttctagt
     3961 acgaaggaat ctgccgaagt atcatcaggc gcttctgaac aagtatatac agatccaaaa
     4021 gaaatgaaag aatcttatga tgtcgtgatt attggagctg gtggtgcagg gatggcagca
     4081 gctatcgaag caaaagatgc tgggttgaat ccagtaattt tggaaaagat gcctgttgcc
     4141 ggaggaaaca catctaaatc atcaagcggt atgaatgctt cagaaacaaa atttcaaaaa
     4201 gaagaaggaa tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa aggtggtgga
     4261 ggaacgaatg ataaagaaat gcttcgatac tttgtcgatc actctgctga tgcggtcgat
     4321 tggctagatc aaaacggtat caaactggac aacttaacga tcactggagg gatgagtgaa
     4381 aagaggacac atcgtccgtc tgatggctcg gctattggtg gatacttagt ggaggggtta
     4441 ttgaataacg tccatgagcg ggaaatccct atttttgtca acgcagatgt gacagacatt
     4501 aagaaaaatg gggaaacggt caatgaagtg actgtacatg ttcaaggaga agaaccaaaa
     4561 gaggtcaccg gaaaagcaat cgtagttacc acaggcggtt ttggtgccag caaagaattt
     4621 attgaaaaat atcgtcccga tttagctgat tatattacga caaatcaaga aggatcaact
     4681 ggagatggta tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat ggataaaata
     4741 caaattcatc caactgtgca gcaggatgag ggggtcttga ttggtgaggt tgttcgcgga
     4801 gaaggagcaa tcttggtcga tcaagagggg aaaagatttg tcaatgaaat ggatacaagg
     4861 gataaagtat ctgcagcaat tacagcattg cctgaaaaat cagcttattt gatttttgac
     4921 caaggcgtgc gtgatcgtgc cacagctatt gaattttatg accaaaaagg atatgtcaca
     4981 actggaaaga caatggaaga attagcagaa aaaatcgatt taccaaaaga aacactagca
     5041 aaaacagttg atggatggaa tacggatgtt gcggagaaaa aagatgctca attcaaccgt
     5101 gccacagcca tggaacatga cttatcgtct ccgaattatt atgcgatcaa aatcgcacca
     5161 ggaatccatt acacgatggg cggtgtgaaa atcaatacga acactgaagt attagataaa
     5221 gaaaaccagc caattagggg gttatatgct gctggtgaag tcacaggtgg cttacatgga
     5281 aacaaccgga tcggtggaaa ttcagttgcg gagatcatta tttttgggcg acaagcgggc
     5341 atacaagcag ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat aaaaagaggt
     5401 tgtgaaatca gcgttttgag ctaataccga gggtcagctg attgaacact gtttagtgaa
     5461 gaggttgtga aaaag
//
LOCUS       DAZKNM010000154         5395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000154 DAZKNM010000000
VERSION     DAZKNM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.157"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..287)
                     /locus_tag="ACG8M9_002346"
     CDS             complement(57..287)
                     /locus_tag="ACG8M9_002346"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0379062.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(381..719)
                     /locus_tag="ACG8M9_002347"
     CDS             complement(381..719)
                     /locus_tag="ACG8M9_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379063.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1647..1763)
                     /locus_tag="ACG8M9_002348"
     CDS             complement(1647..1763)
                     /locus_tag="ACG8M9_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0379064.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2239..3117)
                     /locus_tag="ACG8M9_002349"
     CDS             complement(2239..3117)
                     /locus_tag="ACG8M9_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0379065.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(3440..4465)
                     /locus_tag="ACG8M9_002350"
     CDS             complement(3440..4465)
                     /locus_tag="ACG8M9_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0379066.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            4824..5327
                     /locus_tag="ACG8M9_002351"
     CDS             4824..5327
                     /locus_tag="ACG8M9_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0379067.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT         1766 a         1002 c          818 g         1809 t
ORIGIN      
        1 atatcgaaaa aacaataaca taatatgtgt caaaattttt cataataaga actcccttac
       61 tgttgcatac taacataatc caaaaactct tctacttcag ctaaagacat tccactttct
      121 ttagtccaaa aaacagtttt ttctatcgat ccttctctgt aaactgtaaa tgtcggagtc
      181 gagttaattg agtagcgact cttgaattct ttccactcat tttcatcttc tctaactttt
      241 gctacattca cgtaataaat attttctttt cccttttcta ccagcatatc gaatagcttc
      301 ctgcttttga tgatcgattt cttgtgctac atctagtaaa ttttcatcaa tttctatatc
      361 ataaagtatt tcactagtgt ctacatccac aatatatact ttttatcagt agcaattttt
      421 gagtaatccg ttaaaatgat ctcagtctcg tcactttcat cagtataaat atacgttaac
      481 aatgaactca ccttttcaat cggtgttagc tctaagccta ctgtattttt tactattgag
      541 tcattattta ccaccataac ttcgtactgt atataccatc ctatttggtt tccatcaata
      601 tcagtaattg gctcactagc taatggagtc tccggaacaa tattttcctg atatttctta
      661 aactcttctg tagcacgaaa aaggttatca tgttcctcac ttgaaactgg ttgagtcata
      721 gaaaatgtta cttggctaaa cagagggaaa ataaacacta caatcaacaa agtataaaaa
      781 aaagcccttc tttttcattt tttatcttcc aatcacaaat tgaaaaactt attcctgcaa
      841 ctttgagtct agtatcacaa tcgactgact acaactattc atttacattc tactttaagt
      901 tcctccttaa ccattaattt tattaaagat acttgtaata gtgattctca caaaatttca
      961 tgaaaaatac tttattactg tatctaaaat tccaatacac aacttatcga ctattatctg
     1021 aaaaccaaaa cttattattc cttgatgtta ttctagcaat agtaactgct tttttatttt
     1081 tttgcatatt ttttcggcgt tactttttaa agttaacttt ttcgacaaat agaaagagat
     1141 acgaatcact gatttttttg aagaaaagaa atggagtgag ctgaacgtta gagatattgg
     1201 gagctttcgt aactgcttat taaacgataa tggttatttg caaccgtatt gctcgcttct
     1261 acatgaaata tttcatgaaa tatttcgcaa aacctttgat tatgtagtta cgtttccatt
     1321 cttgactaaa aatgtttcaa aaacgaacaa aggtgttaaa aggtaaagtg atcaatacta
     1381 ctggtcaaaa taagaggttg aaagggaatt gaaaaataac tacagataat acttttataa
     1441 acgtacgtgc tttgtggttc tgtagttaac tatataactg gaattaggga atggtatcaa
     1501 tttgaaaaga aagattctac caatttatca tatgttaaaa atggaaaatc aacagcagtt
     1561 ctttcggaaa ccttacacaa aaaccgatta ttgaaaatag ccgaaaaacg tttttcggct
     1621 atttttttaa ttaagtttcc ttcttcttac cttttcccac tattccgttt cacatctttg
     1681 atgatcttca gcgcacgttt tcgtgttttg atatttgggc ttttcaagcg gcggtatgca
     1741 cttgagatat ctgttttttc catatggacg acccactttc ttctttgatt gtagtcatgc
     1801 taaaaaacac aaatcgaaac aattacgatt tgtgttttaa atggtatcaa aagttaaaat
     1861 gaaagtcaac ttgttaaaac agtcgatcag actagctgat tgactcctat ctctggaaat
     1921 aaattttcat tattacgttt tagctaccta aaaaaggccc tcttgtccgt taactttaat
     1981 atctattgtt atagctagtt tacctattga acgtactgtg tgttcgatag aacactaaaa
     2041 aagaggctgg gacagaagcg tttaactcca agaaataaga agaaattcac gaaaattgct
     2101 tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgct tccgccgttt
     2161 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tcttttaaac
     2221 tatttcgaag tactaaactt atgaagtaag aatacctaag ccagagccaa tgtgctcgcc
     2281 tgcaagcaat tgctcttttg cttgtcgctg taaactcgta ctttctggat aatcatgtat
     2341 ttgggcatct actcgtccaa agatccaagg gactccacta tcgtcctgtt catcaactga
     2401 taataatcga taatcggctg caaaaagaca gatttttgac tcttttttca accatttagt
     2461 aagattgggt gatagtaacc aagtatctgt gtacatcact tggtaatggt aatcaggaaa
     2521 ataaagttca aaaaaacgaa tggcactttc gtaactatct cttatcttat tctgagataa
     2581 atctgcatcg gaaggaatat gaagagaaag gcactgcttt ccttctaatc ttagatccgt
     2641 gtaccctttc ggaaaatatg tcatttcgta ctccaattca ccaatccgaa agatcaagcc
     2701 actagtgtaa tgccaaatcc aaaaaccacg gtcaaagcga tagcttcctg ttacttggaa
     2761 atgttcatga ataaatctag taaaagcatt cattgttgcc aaatagatag ttcttggaat
     2821 cttcaatggt tgataaattt ttttccaact ttgagaagct gcatatagat aaatagctaa
     2881 tgctgcaaaa ggttcctctt catagacttt gtctattttc tttgcagtcg cctttgcttt
     2941 attaggatcc attagttcat gactatattg ctcaaacacc tcatcaagga tgttttttgc
     3001 ttgtacaatc atttgctcta taacgacttt gggaagagtt aaagtttctg ccagaatttg
     3061 gatttcattg cttattttct tcatcaaatt tccccttttc atatctttga acctcattgt
     3121 ttaaatgact gattatccgg acaccatatg gtataaacat acttttattg tacactaact
     3181 ataaaactta aactttctat gaagaaagaa tatctgtaag actttcaagg aaagcattga
     3241 ccatctcagg aaagtttatt cagttaccat cataaacaaa attttttgat agaataaata
     3301 taaaattttt ttgaaagttc ctttccacat ttttgtgtga atttgactta atgctgagaa
     3361 tctggctttt gaacaccttg aattagaggt tgtgacaaaa atcttgtcat aacctctttt
     3421 ggcacatata tttgtccttc tattctaatt tttgacgaac aaccgttaaa taatgttgca
     3481 aaccaaccgt cattacccac gcaacaccta aaccgatgaa acttcctatg acgatcccta
     3541 aaaaacgata ttcgatcaaa gaaatcaagt atgctccacg aataagtgta gatagcatca
     3601 acgccatcgg attaagaaaa atattagcaa ctgcataatt tctagcaact agaaattgtg
     3661 cgattccata tagaaaagtg atcagaaaaa tggtctgcaa ctgcgtaaac ggcacaaatg
     3721 acaagaatgc cgacacacat aacccaccca tcgatccaat cagatattgg acttgtcgat
     3781 gcttcatcgc atccaagttt tctgctagca aaatcgatgc acatgacaag gtcaaccaat
     3841 aaggattatg cagattcaag ccgtgactga cataaacgga aagaaataag gcagcgctgt
     3901 agaaaacaga atcaatgatc actaaaggct cttcgtgaaa tctttctttt aaagttgctt
     3961 tttcaattgt ctgctcaggc cgtgaatcca gtaattttgt aatcactgcc gcaatcaggg
     4021 caaaaacgac acccataaaa aaataaagtg agacgatcgg taattgagcg attggcagct
     4081 gcatgctcgt tcccatggca gaaagcatcg caaaaaacaa gccacctggt ttagaaatat
     4141 ggaataatcg caacacgaat ctgctggaaa aagcaaccaa agctacggca aatggttcaa
     4201 gccataaaac ataagtcgat aagatcccca aaacgaaagc aaccaacagt ccgcaaccga
     4261 tcagaaacat acgtttcagc aaatatttca tcggtatgtt ttgataatag aaaaaagtaa
     4321 agatccctaa tgatgaaaaa cttgaaatca aaaaatcatg atttatgtaa cctaaccata
     4381 atacaataaa gataagtagc atcagattca tgatacgaaa aggattatct tgtattttat
     4441 tgaaacgaaa ataagtcttg aacaatctat ccctccttcc tagtaattgg aaaaaaagct
     4501 gaaccaagat gaaatgccaa tcccgtttgt cagcattacc tagtataaca actatttctt
     4561 gatttttcat aaacttagta tcaaaaagta aacaacttgg agaaattaga acaaaaaacg
     4621 aacattccga gtcgttttga cagaaaaaag atagcaactt ttccaagcaa ctcgctatct
     4681 tagctttgtt aaagaaaccg gattcccaac aaaaaaaagc ttctaatttt cgtacgataa
     4741 ctacgttggt ccttaaactt catacttttt tatgattttt cagcggtttt cgatggtata
     4801 ctttttaaat ggaggagatg tctatgcggt atgtatcaaa atttttatta gggatttatt
     4861 tattctttct attatggttg attttattca agctatcatt taatcttcca caatttctca
     4921 cttattctta cagcaatgtc aatctcgtcc ctttttctac tttttctttt gaaaatacga
     4981 cggtattacg tgagaccact tataacttga ttgtcttttt tccttttggg gtgcttttga
     5041 atgtcaattt caagagactt tctttttcta aaaaattagg gattattttc cttgtaagct
     5101 tcttggcaga attgattcaa ttcctattcg gtattggcgt agcggacatc actgatttga
     5161 taactaatac aacaggagga ctgataggtt tatgggccta tcaacttcta aacaaacatc
     5221 tatcgacaaa taagttagac cgtctagcca ttattttggg atacatttta ttcttatttc
     5281 tgatcgtagc ttttttattt ttgacccgaa ctgtccgtct tcaataaaat cgttcgtaaa
     5341 aaaagaccgt aatagacaca ttccaatgtg tatctatgac ggtcttttta ttcag
//
LOCUS       DAZKNM010000155         5288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000155 DAZKNM010000000
VERSION     DAZKNM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.158"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..234)
                     /locus_tag="ACG8M9_002352"
     CDS             complement(<1..234)
                     /locus_tag="ACG8M9_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379068.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSS"
     gene            complement(532..1506)
                     /gene="sgrA"
                     /locus_tag="ACG8M9_002353"
     CDS             complement(532..1506)
                     /gene="sgrA"
                     /locus_tag="ACG8M9_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0379069.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1702..2541)
                     /locus_tag="ACG8M9_002354"
     CDS             complement(1702..2541)
                     /locus_tag="ACG8M9_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0379070.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2726..3613
                     /locus_tag="ACG8M9_002355"
     CDS             2726..3613
                     /locus_tag="ACG8M9_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0379071.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4207..4902)
                     /locus_tag="ACG8M9_002356"
     CDS             complement(4207..4902)
                     /locus_tag="ACG8M9_002356"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0379072.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4886..5284)
                     /locus_tag="ACG8M9_002357"
     CDS             complement(4886..5284)
                     /locus_tag="ACG8M9_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0379073.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1681 a         1068 c          777 g         1762 t
ORIGIN      
        1 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
       61 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
      121 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
      181 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
      241 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
      301 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
      361 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
      421 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
      481 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
      541 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
      601 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
      661 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
      721 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
      781 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
      841 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
      901 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
      961 gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt caccgctcgt
     1021 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     1081 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     1141 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     1201 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     1261 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     1321 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     1381 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     1441 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     1501 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     1561 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     1621 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     1681 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     1741 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     1801 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     1861 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     1921 ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     1981 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     2041 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     2101 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     2161 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     2221 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     2281 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     2341 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     2401 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     2461 tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa cagatccaat
     2521 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
     2581 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
     2641 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
     2701 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
     2761 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
     2821 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
     2881 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
     2941 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
     3001 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
     3061 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga
     3121 taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct
     3181 attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt
     3241 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
     3301 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
     3361 aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag
     3421 ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa
     3481 atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa
     3541 aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa
     3601 tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt
     3661 ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt
     3721 tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa
     3781 aaagtttttt atttctatca catgaatcaa tgcgccatta agtagattct ttcatgcttc
     3841 cctatttttt cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg
     3901 aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt
     3961 atactagaga aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt
     4021 tattcaaaac tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc
     4081 gtttaaatga gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata
     4141 ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa
     4201 attgattcag aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt
     4261 ttgcacccaa tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac
     4321 ttgatctagt agctctttac ctaacgttat cgcatgagct ccagatcgaa tggctgtagt
     4381 gacttgttca caatttttga aactagctgc taatattttt gttggtcttt gcgtacgttc
     4441 gatctcagtc cgcaacgaat caatcacttt catagcatca atgccttgat tttgcatcct
     4501 gttataataa ggtgcaatat aatctgctcc attattaata gctaaatacc cttgtaattc
     4561 tgtgtaaata gctgtaccgg taatcaaata accttctttt ttcaactttt tgatcgcttg
     4621 aaggcctgct tgattcaccg gcaccttgat aaacgtatct tttcccagtt cttctatgat
     4681 aactcgagca tcctctatca tttcttcttc cgttcttcct attgtctgaa tatggatagg
     4741 aatctgtccg attatttttt tgatttcttt catatgcgag aagaagtttg acgctccttc
     4801 ttttttcaga attgtaggat ttgttgttac gcctgttaaa ttcaaaaact gttgacaaga
     4861 ataaatactt tttgtatttg ctgtatcaag catgatttcc atatagcttt cccccattct
     4921 gatcctcttg atcgatttta atgattgtcc cctttcttaa atgcggcatt tccttatctt
     4981 ctacatacat tgttcctgga agttccggct catgagatcc atcaaatttt atcgtgatat
     5041 gtcctaattc attcaaatta gtttggacaa catctcctac tgctgtgatt acatacattt
     5101 gtttatcaaa acagagcttc atatcacaag ttatttcttt tcttacctga ttcaattcaa
     5161 tcttataaca ataatctgtt aatgtttccg gtgcttcatc tccaaataaa atcattattt
     5221 tttctttcct gaatacttct gcttctatac caacatctgt tacaaccgtt tcaaagatcg
     5281 tcatctct
//
LOCUS       DAZKNM010000156         5283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000156 DAZKNM010000000
VERSION     DAZKNM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.159"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1881)
                     /gene="glmS"
                     /locus_tag="ACG8M9_002358"
     CDS             complement(76..1881)
                     /gene="glmS"
                     /locus_tag="ACG8M9_002358"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0379074.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2290..2580
                     /locus_tag="ACG8M9_002359"
     CDS             2290..2580
                     /locus_tag="ACG8M9_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379075.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2593..3618
                     /gene="sppA"
                     /locus_tag="ACG8M9_002360"
     CDS             2593..3618
                     /gene="sppA"
                     /locus_tag="ACG8M9_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0379076.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3621..4280
                     /locus_tag="ACG8M9_002361"
     CDS             3621..4280
                     /locus_tag="ACG8M9_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0379077.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4437..>5283
                     /locus_tag="ACG8M9_002362"
     CDS             4437..>5283
                     /locus_tag="ACG8M9_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379078.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGK"
BASE COUNT         1645 a          961 c         1114 g         1563 t
ORIGIN      
        1 ttaagagata ttttcataaa aaataaaaga acaaggacca gtgtcagatg tctgacactg
       61 gtccttgttc ttttattatt ctactgtcac tgatttcgct aaattacgtg gtttgtcaac
      121 gtcgaatccc cgtaatagcg ttgcgtaata tgcaagtaat tgagtcggta cgactgtggt
      181 tagagtagtt agataagggt ggacagttgg tagcaccagc tggtcacttt ccttttctaa
      241 tccctctgag acaataacta gtgttttagc gccacggctt tcgacttctt tcaggtttcc
      301 tcgagtatgc gcaccagtca cttcttcact gatgattccg atcactggtg ttccttcttc
      361 gatcaaagca atcgttccat gtttcaattc gccagcggca aaaccttctg cctggatata
      421 actgatttct ttcaatttca aagcagcttc aagagaaacg ttgtagtcag caccacgtcc
      481 aatgtaaaag gcatttcttg tatcgctgag atatacttct gccaactctt ctaaataatc
      541 tttttgggcg atgaccgatt ccatcgcatt agcaactaaa ctcaattcat gagtgatatc
      601 gaatacaaga gaagtttcaa attctttttt atcaccgata gctttggcta ataaagttag
      661 tacagctatt tgtgcagtat aagctttcgt tgatgctact gcgatttcgg gtccagcatg
      721 caatagcagt gtaaaggatg cttcccgcga aagagtagaa ccagcaacat ttgtaatcgt
      781 aagtgaaggg tagccgagac ggttgatttt taccagcact tggcggctgt ctgctgtttc
      841 gccactttga ctcaagaaaa taaagaatgg tttttttgaa agaagaggtg tgttatagcc
      901 aaattcactg gctaaatgaa cctcaacagg aatctgtgtc aatttttcca aagtctgttt
      961 acctgctaac cccgcatggt aactagttcc gcaagcaacg atatagattc gatcgctatc
     1021 agacaatgta tcgaccagct tttgatcaag agagacacga ttgtcttctg ttacgtattt
     1081 ttgagcgata cgacgcatga taataggctg ttcgtcgatt tctttcagca tataaaaagg
     1141 ataggtacct ttctcgatat ctgaagcatc tacttgtgct tcgaatgaag aacgttctac
     1201 gatgtcacct gcaaaatttt gaataataac tttttctgat gtgacggtaa ccatctcttc
     1261 atcttcaatc tcaacgaatt ccttcgtatg agcaagcata gccattgcat cgctggcaat
     1321 cacattgaat ccatcgccga gtccgattaa tagtggactt ttatttttag cgacgtaaat
     1381 aacatcaggt gctgtccgat caatcaaagc gaaagcgtaa gaacctttga tgacccgcaa
     1441 agcttttaaa aacgcctctt gcgttgtagt atccttcgcg aaagtttcaa ttagatgagc
     1501 gataatctca gtatcggtct caccaataaa gtggtcattt gctaaataag caattttaag
     1561 ttcttcaaag ttttcgatta cgccgttgtg aacgagcacg aagcgtccac tttgggaagt
     1621 atgtggatgg gcgttttctt cagaaggttg cccgtgagtt gcccatcgtg tatgaccaat
     1681 acctatattt cctgtagtat caatatttat tttgtttttc aaattttgaa ttctgccttg
     1741 tgctttgacc agataatcag ttgtgccatc tgagacaaag ataccagctg aatcataacc
     1801 acggtattct aatttttcta gaccgtcaat caatcctggt gttacatttt ttaatccaat
     1861 cattccaaca attccgcaca taaaaaacat cctactttct ataatgaatt cgtatgaatg
     1921 cttccagctg caggaaaatc cttttgtttt atctttgcag acattttttg tggattttct
     1981 gtagagtgca gtccctaggg ccatccgccg aatctttcga taaaccctct cctcgtcatc
     2041 tgtttacaca gacctggcgc tatcctaaaa ggtataacta tcctcctttt tgttttttat
     2101 ctggaaacat ttaaaatcat aaccttttgc gaatgttctt gtcaacaaaa aaagaataaa
     2161 aaataaattg gtatatatca aattctttgt caaatgtcct ttttatgtca aaaaataatg
     2221 ggtttttatc tatcatcttt ttcaggagtt cggtacaata gaaagtaatc ggatagagga
     2281 ggagtaaaaa tggattcaaa aattgagatc atgacactgg gaatgttaaa aaaacaattg
     2341 tccaaattcg aagcatcagc tggtgtatca gatgatacga aaatattttt agatactggc
     2401 tgggatagca tccaagaaat cgcacctgat gccttggaag ttgctcaagc tcgtgagttt
     2461 acggtagaag atgaattaac aaaagagtcg ttttccgggt atgcgcggga agaaaaagca
     2521 gagcggttcg acacttctga gcagtcagaa actgtgatcg tcataaaaaa cttgtattag
     2581 aaaggagaaa gaatgaataa aagacgttgg gcagcagttc tgattgctgc aggattattt
     2641 gtggtttcgt tagtttcagc tagtttgact aagcctcagg aaaacgatca aattgaggga
     2701 ttgaacagtt ggttatatgg ggatgaagaa ctagctccaa atgtgttgga agagggagac
     2761 agtacgcaaa aaatcgtaaa attatcagta gatggaacga ttgctgcggg agggtcttca
     2821 ggtttatttg cttctgaagg ttacaatcat aaaaatttta tggaacaact gaaggcgatt
     2881 gaagaagatc caacagtcaa aggtatcttt ttagaagtaa attctcctgg tggcggtgtc
     2941 tacgaaagtg cggaaatcgc taagaaattg gatacgattc gtaaagaaca tgatatccca
     3001 atgtatgtca gcatgaaaaa catggcagct agcggtggct actatatttc tgctcaagca
     3061 gataaaatct ttgcaacaga agaaacagtg accggctcaa ttggtgtgat catgtcagga
     3121 ttgaattatt caggacttct agaaaaactg ggtgtggaag atacaacagt taaaagcgga
     3181 gcgttgaaag atatgggttc aggaacgcga ccagagacaa aagaagaaga agcggtgctt
     3241 caagcatata tcgacagcgc atatagtcgt tttgtagcaa tcgtcagcga gggacggaat
     3301 aaatctgaag aagaagtcaa aaaaattgct gatggaagga tttatgatgg tgtccaagca
     3361 aaagaagtag gtttagtcga tgaattggga tttcctgaag atgctttaaa agctatacga
     3421 aaggaacaac agcttgaaga tgcggaacta gtagaatatt cttccacgcc atctgggttt
     3481 gctagtacat ggttaggaag taaactagca gaattccagg gattgaagcc ttctgagact
     3541 agtcagatct tatccgttct tgagaatcta gggacagcgg aatcaccaaa agcaatgtat
     3601 tattacggag gtgagtaagc atgtcagagc tttcaaatga aaataaggaa caagaaaaag
     3661 ataagcaaca gcagcctgct gtaggacctg actccaactt ttcccaaaag gtactgagct
     3721 cttttgaaag agagcctttg acgcccgaag aaatcaaaaa gaaacaacgt aaatggaaaa
     3781 agtatacgga cacaagaaat gaaccgccaa aagcaaatga ttttcctaag tatttctatg
     3841 caggtttttt tatccgaatg tttgccttct tggtcgatct tttgtgtatc ggagctatta
     3901 cgcgtttaac acttggtatt gccaaaaatc taggttggct taatttttca gattcttatc
     3961 tgagtctcta cggattcaca gccttactta tttatctagc gtattttatt ttattgacga
     4021 aattcaatca tggccaaacg attgggaaaa tgatctttgg tattcgagtc atcagctttg
     4081 atgaagaaga acttagctgg tcaaccgttt tagttagaga aggtgcttgc cggttcattc
     4141 taaaatttcc tttgctgatg ttagggtatc ttccagcagt ctttaatcag aaaaagcaac
     4201 atctgggcga ttttttcact aatacaagtg ttgttacgat caatctgatc aaagcgttca
     4261 accaggaagt caacagctga tgagcaataa aaagcagtaa cagttgaaag caccttctaa
     4321 aagtcagatc taaaatctag cttttagagg gtgcttcagt tatttttaat tgtcatgctt
     4381 tttttgtttt gctactattg aacatgagct gaatatgagc aagaggagga cactgcgtga
     4441 agccgattga ttttccagaa aaatatgaga atttaatgcg agtggcacaa caagcactag
     4501 caaaccagca atatcagcag gcaaaagaat tatttcaacg agcttacgag ttgaaagaaa
     4561 gctttgaggc taatagcctg cttgtttttt gtctttacga gttggacgag aaaaaagaag
     4621 cattgaagca agcactcttg catgaaaaac agtatcttca aaatgaggag tttgctgaat
     4681 tttattttga cttgttaatc agtacacaag attttttata tgctagaaaa ttgatagcaa
     4741 gtacagattt ttatgaagct ttcgagcagc gggtcattga aaaaatccaa tttgcagaag
     4801 aattatccgg acaaatggag aggcagaaga tcaaggcact ccacaataaa tcggaggaac
     4861 ttccttcgtt ggaacctgca cgtcagcttg cgtctattga tgagatcgaa cagcttcctt
     4921 atcatgagtt tatccaaaca gcgagtaaac ttattgtatc gccagaggtc catattttag
     4981 caagggcaaa attactggag acacttaggc aattgaacga atgccatcaa gttttctatt
     5041 tgacgattga agaaaagtta gtcaaagtca taccaaaaga tctgccaaag ccgcagcagc
     5101 aggcaagtta ccagcaatta tgtgtatttt cagatcgtta cgggaatgaa gatgcgctgc
     5161 ttagttccgt gttaaaagaa gaattcacgt tgcaatctgc aattgtttat cctgtctatg
     5221 atacatacat cgaagacccg aagcgctggt ttcaactgac tgtagaggcg tgtacaggaa
     5281 aaa
//
LOCUS       DAZKNM010000157         5192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000157 DAZKNM010000000
VERSION     DAZKNM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.160"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1122
                     /locus_tag="ACG8M9_002363"
     CDS             4..1122
                     /locus_tag="ACG8M9_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0379079.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1250..2626
                     /locus_tag="ACG8M9_002364"
     CDS             1250..2626
                     /locus_tag="ACG8M9_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0379080.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3027..4607
                     /locus_tag="ACG8M9_002365"
     CDS             3027..4607
                     /locus_tag="ACG8M9_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0379081.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4805..5029
                     /locus_tag="ACG8M9_002366"
     CDS             4805..5029
                     /locus_tag="ACG8M9_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0379082.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         1742 a          845 c         1079 g         1526 t
ORIGIN      
        1 ctattgtttg aaaaacttag acagtctaaa ctaatgtttt ggtcgcttga attattgatc
       61 atagctgcat tgatcatgat ttcatcagaa ataaacttta ttttcgaacc gatcgggaca
      121 tttttttcca cgttatttgc acctgtactg attgcaggct ttctttacta tttattgaat
      181 cctattgtca agctgttgat gaaagcaaaa atcaaaagaa tatgggctgt cgcaattgtg
      241 ctgctccttt tagttgcagc aattatttgg attttactta gtgtcatccc aagtctagta
      301 cagcaaattt cctcattggc atctaatatg cctgatttca tcaaacaggt agaaagctgg
      361 ttaaaagagg tagccgaatt gcctttgttc aaggaagtag atatcaataa atattttgag
      421 caattagaca tttcttacgg gacaatcatt cagcaattcc ttagtggagt atccaatagt
      481 ttaggttcga tcgttggaac gatcgcttcg gcaaccgtag taatcattac agcaccattc
      541 atcttgttct atatgttgaa agacggagaa aaactagtgc caaacatcca acagtttttc
      601 ccagaaaaaa gaagaaacca aattgtggag ttattggggc aactgaataa aacattggct
      661 aattacatta gcggacaagc aatcgaatgt ttattcgttg gtaccttcac atttttagga
      721 tatatggtaa ttggtgtgga ttatgctttc ttatttgggg tcattgcggg attgacaaac
      781 ctaattccat atttaggacc ttatctagga ctagcacctg catttctagt aacagtcttc
      841 aatgatccgg taaaagcatt actttgttgt gtggttgtgc taatcgtcca acagttggat
      901 gggaatatca tttatcctaa tgtaattggg aaatcactga aaatccatcc attaacgatc
      961 attatcgttc ttctagtagc tggaaatatt gccggattgc ttggtatctt cttaggtgtt
     1021 cctttttacg caatctgcaa gacgatcatt tcctatataa tcaaaatcgt caaagaagac
     1081 aagcaaaatg aaaacaagaa gaaaattgct acgacctcgt aggtagtttt agtacacttg
     1141 tttttattgc aaagaaaatt gctttgtgat accattttaa cgtggctata gtgccacgtt
     1201 tttttctatc gtaaactaaa aattaatttt ataaggagag aatgcgatta tgaatgctga
     1261 ccctgagagt cagtcgctgt tagcgcagat tttattattg atcgttttga cattgatcaa
     1321 tgctttttta gcagcttccg aaattgctgt tgtttcgatc aataagaatc gaatcgagca
     1381 aaaagctgat gaaggagatg tgaaatctca aaagctttta aaaatattgc agaatccaaa
     1441 taatttccta tcgacaatcc aagtaggaat cactttggtc aacattttgt ctggtgcttc
     1501 actagctaat actttatcgg aacggttagc tccggtatta ggtggcggca cagcggcaaa
     1561 aaatattgca aatatcattg tgctggctat tttgacctat gtatcgattg tttttggaga
     1621 attgtaccct aaacgtattg ctttaaataa atcagaagaa gtcgcaaagt tcacttcagg
     1681 gctgatacgc ttgattggtg tagtagctaa gcccttcgtc tggctgcttt ctgcttccac
     1741 aagtttattg gcgcgtatca cgccgatgac ttttgatgat gaagattcaa aaatgaccag
     1801 agatgaaatg cgttatatgc ttgaaaacga aggcgtattg aataatgaag aattagaaat
     1861 gctccaaggg gttttttctc ttgatacgaa agttgcccga gaagtcatgg tcccacgaac
     1921 ggatgcattt atgattgata tcaacgacac tgtggaagaa aatgtgaatg aagttttatc
     1981 cgaaaactat tcaagaatcc ctgtttataa cgaagacaaa gataaagtag tcggaatcct
     2041 tcatacaaag aatttactaa aagctgccca taaattcggc tttgataatt tagatatcaa
     2101 gaaaatcatg caggagcctt tattcgttcc tgaaacggtc tttattgatg atctgttata
     2161 tgaaatgaaa aagacgcaga atcagatggc tattttatta gatgaatatg gcggcgtcgt
     2221 tgggttagca acactagaag accttttaga agaaatcgtc ggagaaatcg atgatgaatc
     2281 agatgaagtt gagaatcttt atgaaaaaat cgatgaacac gaatatatca tccaaggacg
     2341 tatgctgatt gatgagttca atgaagcttt tgacagtgat ctgcatatga gtgatgtaga
     2401 tacgatggct ggttatttga ttacagcatt aggcatgatt ccggatgaag gcgaaaagtt
     2461 atcgtttgat gtcgataata ttacgctggt ttctgaagaa atggaaggtt ctcgaatcct
     2521 taaaatccgt gtcatttttc atgatccgga agaaacagaa gcggaacctg aagaagaacg
     2581 aagatacttc agaaaagaat tcgaagatga cgagccaaga agataattgg aaacttggtt
     2641 atgaaagacg tgttgcttga ataaacaagc aacacgtctt ttcttttagt cagctgacaa
     2701 aaaaatattt gactcttatg gtttggtggc tttttcattc gctgaatcca tgctatactg
     2761 attcagttgt agtagttgac aaaggttgtg aaaaagctat cctgcattaa gtaataagag
     2821 cagccaagca aaaacagctt ttcgtatttt tggcttgggt gggcttattg ctgataggct
     2881 gtctctagaa cgccgtttac tcggaaaaag agactgctac acggcttttg tcacagccct
     2941 atatgaaatt gtctttcttt aagagatcgg acaatctgtg cctagagaaa atataaataa
     3001 ttgaatggaa gaaatggagt taggttatga ataatccaaa attaaagcaa caagtggaca
     3061 atcgcagaac ttttgcgatc atttcccatc cggatgctgg gaaaacaacg attacagaac
     3121 aattactatt gtttggtggg gctatccgtc aagccgggac cgtaaaaggc aaaaaaacag
     3181 gtaattttgc caaatcagac tggatggaaa tcgagaaaca acgtgggatt tctgttacta
     3241 gttctgttat gcagtttgat tatcaaggga aacgagtaaa tatcttggat acaccaggac
     3301 atgaagattt ttctgaagat acttatcgta ctttgatggc cgtggatagt gcggtcatgg
     3361 taatcgatag tgcaaaaggg atcgaagcac agacaaagaa attattccaa gttgtaaaaa
     3421 aacgtggcat cccgatattt acttttatca acaaactaga tcgtgatggt cgtgaaccat
     3481 tggaattgtt agaagaatta gaagagctat tagatatcga atcttatccg atgaattggc
     3541 caatcggtat gggtaaaggg ctagaaggtc tttatgatat ctaccataat cgtgtagagt
     3601 tttatcgtcc ggaaaattat caggatgaac gattagtaga attggatgaa gatggactgc
     3661 ttcccgaaaa tcatcctcta actgaaaatt ccttatatga acaagtctta gaagaagtag
     3721 aactaatcaa agaggctgga gatgcattta atcaagaaaa aattgctcgt ggcgatcaga
     3781 caccagtatt ttttggttct gcattgacta acttcggtgt acagactttt ttagagactt
     3841 tcgttgaatt ggcaccagct ccttatggac acaaaacaca agatgatcag atggtaagtc
     3901 cttatgagga agaattttca ggtttcgtat tcaagatcca agcaaatatg aatccagccc
     3961 atcgtgaccg aatcgctttt gtccgaatct gttctggaac attcgaacga ggaatggatg
     4021 tctggttgga acgtacaaat aaaaaactga aattaagcaa tgtcacacaa tttatggcag
     4081 attcgagaga aaacgtcgaa aaagcagtag caggcgatat catcggtgta tatgatacag
     4141 gcaattatca gattggggat acattgttcg aaggaaaact aaaagttgct tatgaagaac
     4201 tcccttcatt tactccagag ctgtttatga aagtgactgc aaaaaatgtc atgaaacaaa
     4261 aatccttcca caaaggaatc tatcaattag ttcaagaagg agcgattcag ctttataaaa
     4321 catatctaac ggaagaatat atcattggag ctgttggtca gctacaattc gaagtattcc
     4381 aatatcgtat gttaaatgag tataacgccg aagtaatcat gtctccaatg ggacacaaaa
     4441 tcgctcgctg gatcgatcca gaagatttgg atgagaagat gagttcaagt cgcaatatct
     4501 tggctcgtga ccgtttcgac caaccgttgt tcttattcga gaaccaattt gcagaacgct
     4561 ggtttgcgga taaatatccc aatgtaaaat taaaaagttt gatgtaaaga agtcttgaca
     4621 caagctgccc tgcatcaagt aataagaata gccaaacaaa aacagcctct catattttgt
     4681 ttagagccgt gacggatttt ccgtgacggc tctttttact atattaaaat catagaaatg
     4741 tctaatcaaa aaaatataaa aattattttg cgtacatatg attaaagaaa ggagagtgtg
     4801 acacatggaa acagaatggc aaagacgtga aagaaaaaaa cagaaaaaag aagagatgtt
     4861 acgattatgt cgattattag atgtaaaaga ctgggacaaa aacgagcaaa ttgcacaaaa
     4921 agtacgatca atcatccata aagaggaaaa agttaaaaat aaagacacgc gtagaactga
     4981 aaatgaaaag agaaaagtac aaaagaaaag agaaaaatca aaaagctaga aagaaaaaga
     5041 ggctgagaca gaagcgttta actccaagaa ataagctgga attcacaaaa atttgaaaag
     5101 caattttcgt gaatttcagc ttatttccga aggagttgct tctgctttcg ccgtttatac
     5161 gtgtttagag cgtgagacaa aagtgttctt ta
//
LOCUS       DAZKNM010000158         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000158 DAZKNM010000000
VERSION     DAZKNM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.161"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..746
                     /locus_tag="ACG8M9_002367"
     CDS             135..746
                     /locus_tag="ACG8M9_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0379083.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            819..1748
                     /locus_tag="ACG8M9_002368"
     CDS             819..1748
                     /locus_tag="ACG8M9_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0379084.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1885..2643
                     /locus_tag="ACG8M9_002369"
                     /pseudo
     CDS             1885..2643
                     /locus_tag="ACG8M9_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2691..3893)
                     /locus_tag="ACG8M9_002370"
     CDS             complement(2691..3893)
                     /locus_tag="ACG8M9_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0379085.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3973..4497
                     /locus_tag="ACG8M9_002371"
                     /pseudo
     CDS             3973..4497
                     /locus_tag="ACG8M9_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4704..>5189)
                     /locus_tag="ACG8M9_002372"
     CDS             complement(4704..>5189)
                     /locus_tag="ACG8M9_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0379086.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1641 a          877 c         1003 g         1668 t
ORIGIN      
        1 tctaggaaaa aattaactag caccacgggt tatgatataa taaaatgtat tataggcatt
       61 tttttacaag aaatagaagc ttgaagttta tatgaagtta taaaatttaa tttatattgg
      121 aaggaggaaa ataaatgtat tttatactac ctacacttta tagcctttta tgtgtaatgg
      181 caccatgtat tatttatatg gtattttttt gcaaaaaaaa taccctgaaa cacaatatcc
      241 tgattttgat ttttatgctg tactgttgga aagtttttga attgactggt acaggtggac
      301 taacagatat tttttataga cctgatacag gtaggaaatt ttctctagta cagggcagtg
      361 ttaatcttgt accattcagt ggactaaaca ttagctttat attaaatata gtaatgtgtg
      421 tgcccttagg attcttacta ccaatcatat ggaaaagtta taggaaaata gttcctgttt
      481 tcttggctgg ggctctattt tcactaatta ttgaagtctc acaaattata actacaagag
      541 caacagatat tgatgattta atagcaaata cctttggtgc ggttataggc tttatactgt
      601 gggctgtttt aagtagagtg gtgtccttga aggacaagtc atctacagta gattatttag
      661 aaccttatat ttatatattg attggtttta ccggaaaatt cttactgtat tttcctttct
      721 ggtttgcttt tgaagtatta tcctgataat tccatagaaa ataatgagtt tgagttgaga
      781 aagaatattc ttgtacacaa ctctggaaag gaggaagtat gaaaagattc ttaaaatggg
      841 ggcttgttct tttagcgtct ttgattgtga ttctaatgtg tacagcggtt gttatttctt
      901 atgtgaatcg gccgtatgca agagctgttt atgataggat taaacgcatt cttgacaaag
      961 atttttatga ccatctatat ctaggttctt catttgaaat aataaacctt gatacaatgg
     1021 atattcatag ctatatgaaa aaagaccaag aagaattacc cgcctcattg agcaaattat
     1081 ttacgattga ttatgcgctt agtgtcctaa gtttagatga acagattaca attagtccta
     1141 ctgcacttga catgcagaaa gctgactcat ctactgcaca tcttcaagaa ggagatattt
     1201 tatctgttta tgacattatc tgtgctatgg tcttaccctc aggaaatgat gcagcttata
     1261 gtttggcaat ttatacaggt ggaaaacttg atcctgatgc aaagaatgat gaagaacgct
     1321 atcaaagctt catgaaaaac ttagatcgtt atctaagtga gcaaggctat aaacatactc
     1381 attttactga cgctgctggc tatgactcca atgctacaac atcagcggaa gatttgacaa
     1441 aagtagctgc aaagctacta aagaatgata aatttgcaga aatagttgcc aaggaaactt
     1501 atagagcaaa atcaattaag ggaaatgttt ataactggaa tactacgaat caatttatgc
     1561 aacaagataa tagatacttt actgaaggtg taaaaggtgt aaaaactgga tcgtttgatg
     1621 ggagatacaa tctatgtgtg tattatacat ctagttctga taaccactat ttacttgtaa
     1681 gttttggttc agagagtgat agtaggcgtt atgtagatgt tcaaaatcta ttgcataatc
     1741 tgccttgaaa tttcgtgcct ttaagcgcgt atctaaagtt tctttgaaaa gtttgagaat
     1801 gaagaaagtg agcagtcgaa aaacggttgc tcactttttt gccctaaaat cgaaaaaata
     1861 taaaaaagtc ctttacaaca cgtctcagga aaaactgcta agtcaattct aatatcttgt
     1921 ggagcaagac ttgcaccttt tgatataaga gagcttagag aactaatgag cgaagatgaa
     1981 ctagaactcg ataaaattgg agatagaaaa actgccttgt ttgtaataat atcagataca
     2041 gatgatacat ttaattttgt agtttcaata atgtattctc aattatttaa tctactatgt
     2101 gataaggcag atgatgtgta tggtggaaga cttccagttc atgttaggtg tctacttgat
     2161 gagtttgcaa atatcggttt gattcctaaa tttgaaaaac taatagcgac aattcgttca
     2221 agagaaatat cggcaagtat aatactgcaa gcacaatctc aattaaaagc aatttataaa
     2281 gaccatgcag atacaatagt tggtaactgt gactctacac tttttctagg aggaaaagaa
     2341 aaaacaacag taaaagaatt atcagaaacc ttaggaaaag aaaccataga cctatataac
     2401 acatcagaaa caagatcaaa tcaaaaatct tttggtctaa attatcaaaa gactggcaag
     2461 gaacttatga gtcaagatga aataactgta atggatggtg gtaagtgtat ttaccagcta
     2521 agaggagcaa gaccttttct atctgataaa tttgatatta caaagcataa gaattacaaa
     2581 ttgttggaag attatgataa gaaaaacttg tttgatgtag aagagtattt aaaacaaaga
     2641 tagcgtaaaa tattttgtgt aaataactcg tccttctgca aaataatggg ttactcagta
     2701 aacattgaag ctaatgtatc cgtcacttgt tggaagcctt tatggcttct atttaagaat
     2761 ttttgattgt atgtatcaaa gatggatact aggaaacgct ctagtgattc ttcattttga
     2821 aactgctctt ttctgtggct gtatttcttg atttgcttat taaaggattc aatcaagttc
     2881 gtagagtaaa tagtcttgcg tatggctaat ggaaaatcat agaaagtgag caggtaatcg
     2941 ttcgaaagga tcgattccac catttttgga taactggttt tccatttttc cgcaaaagca
     3001 ccacgtgctt ccaatgccgc ctttttagat gacgcttgat acaccatttt aaaatcatca
     3061 cagacttcct tacgatcatc gacacgcact ttgtgactga tattacggga aacgtgcaca
     3121 cagcaatgtt gaaaacgggc tttggggtag aaccgctgca cggcccctgc catccccttt
     3181 aagccatccg tgatgaaaag gaggacgttt ttcacgccac gttcctggag gtctattaaa
     3241 atctcttccc aaatcgtgat agattcagtt ggtgcaatcg catagctcaa cacttccttt
     3301 gaaccgtccg ggcgaatgcc aactgcgata tgaatggctt ccttggcgac ggttttccgc
     3361 tttaaaggga tataggttgc gtccatataa atagcggcat aacggtcatg aagctcacgt
     3421 tccttaaatg aagtgacttc ttccgtaaat gatttagtca tattggacat ggtttggggc
     3481 gtgtagtgat gcccatacat tttctcaatc aagtctgcga tttccgacat ggtaatacct
     3541 tttcggaaga ggtgaatgac ggtctcctct aaggtatcat tcgtccgtct ataagcagga
     3601 acagtctgtt gcttgaactc gccgttgcgg tcgcgcggaa tctggagatg aagttccccg
     3661 tactcggtct tgaccgtacg gtcataggag ccgttacgag aattacccgt gttaaaacca
     3721 atgcgatcgt acttttcgta atcgaggaaa gccgttaact ccgttttgag aagcgtattg
     3781 acagcttttt ccaaatggac acggaataat tcattcaaat cgcctttatt cgctagagtc
     3841 tttagaattt ctgtagtaaa atcgttcata tggaagtcct cttttctgtg aatgtgttgt
     3901 ggtaactcta ttctactgaa gggacttcct tttttctatt tacacaaaat attttacact
     3961 ctcggattga aagaacgcgg tctttcagga gtaaagatgg tcatctcgga tgcgcatata
     4021 gggctcacaa aagctgtgaa agaatacctt ctttgtgcga gctagcagag atgccaagca
     4081 catctccttc gcaacatttc cgacaaattc tccaagaaaa tctcaagcga tatcaaagaa
     4141 gactttaaag ccatcttcaa aacggcaagc atcgagctgg ctggagaacg taaacaactt
     4201 attatggata aatatgggga agacaagaaa gtcgcaactg cctgtaaaat attggatgag
     4261 ggcttcgaag atgccatgca agtaatggtt ttaccagaaa atatccgtca acgattgaga
     4321 acaacgaatg ttcttgaaag actaaatgaa gaagtacgcc gtcgtgaacg tgttgtccga
     4381 attttcccaa attgtgattc tgttattcgt atcatcggtg ctttattgat ggaaaaggat
     4441 gacgaatgga cttctgcacc tagaaaatat cttgaatttg atcgtaacga gatttagcac
     4501 attaagccgc caatagtata atatactggt gagaagtatc aagagacgct tcgctttttg
     4561 ctcttgacac ttcttaccag catattggac cgtagctagg ctcaacaagg caaaacatcg
     4621 tactttgaga tttccttttt acacaagatt ttggacttga cacagcccct atggttctta
     4681 ccttgtttat gcttatttaa atcttacttt attacagtac tttcaaactt cgttttgtaa
     4741 gccgggtatt ttgatatttc ataattatgt ccctcttttt gaatatattc tttgatatct
     4801 gcttgttcat ctacactttc tttgcgtaaa atacgttcga tcattgagca gcagtgcatg
     4861 taaagcaaga acaattgatt gtttgaaaat tcgatcccca ttagtttttc gagttctttg
     4921 acggcctttt gtaccaatct caaaaccttc attgcatcaa ggatcgttaa attttctacg
     4981 atcttgttaa tcgagaaatt aaagtattga gcacaattat aggcgataaa tggagcattt
     5041 gattctatca cctgttgttc cttggcgtat tcataaattt tttctgccaa aaaggttttt
     5101 cctgaaccgc ttacacccat caataaaatc ggtaatccgt tatgaggata caagacagcc
     5161 gtcttgattt gatcgatcgc ttcctttaa
//
LOCUS       DAZKNM010000159         5163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000159 DAZKNM010000000
VERSION     DAZKNM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.162"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1792)
                     /gene="groL"
                     /locus_tag="ACG8M9_002373"
     CDS             complement(167..1792)
                     /gene="groL"
                     /locus_tag="ACG8M9_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0379087.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(1843..2127)
                     /gene="groES"
                     /locus_tag="ACG8M9_002374"
     CDS             complement(1843..2127)
                     /gene="groES"
                     /locus_tag="ACG8M9_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0379088.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            2352..3014
                     /locus_tag="ACG8M9_002375"
     CDS             2352..3014
                     /locus_tag="ACG8M9_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0379089.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(3090..4382)
                     /locus_tag="ACG8M9_002376"
     CDS             complement(3090..4382)
                     /locus_tag="ACG8M9_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0379090.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            4552..>5163
                     /locus_tag="ACG8M9_002377"
     CDS             4552..>5163
                     /locus_tag="ACG8M9_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0379091.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHI"
BASE COUNT         1481 a         1107 c          922 g         1653 t
ORIGIN      
        1 gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
       61 tttccgtgaa tttcttctta tttcttggag ttaaacgctt ctgttccagc ctctttttat
      121 ctgtctttca ctaatgatca gttgtagata agcaagttgc tttttcttac atcataccgc
      181 ccatcattga tggatccatt gcaggagctg ctggcgctgc tggttctggt ttatctgcaa
      241 cgactgcttc tgtcgttaat aacaaggcag aaacggaagc ggcattttgc aaggcagaac
      301 gtgttacttt agttgggtct acgatacctg cttcaaccat gtttacccat tctccagtag
      361 cagcattgaa accagtgcct agttccacgt tcttcaattt atcaacaatc actgatcctt
      421 cataaccagc gttttcagcg atttgacgaa ttggttcttc taaagcacga acaacgatct
      481 tgatacctgt tgccacgtct ccttctgctt ctacagcaga aactttgctg ataacgttca
      541 ctagagctgt accaccacct gagaccattc cttcttctac tgcggcacgt gtagcattca
      601 atgcatcttc aatgcgtaat tttaattctt tcaattctgt ttcagtagct gcaccaactt
      661 taacaacagc tacaccacct gctaatttag ctaagcgttc ttgtaatttt tcgcggtcaa
      721 aatcagaagt tgtttcagca atttggtttt tgatcaattg cacacgagct tcgattgctt
      781 ctttttctcc agaaccttct acgatcgttg tattgtcttt atctacaaca actttagaag
      841 catttcctaa gttttcgatt gttgcatctt tcaattctaa acctagatcg tcagtaatta
      901 ctgtaccacc tgtcaaaata gcgatatctt caagcattgc tttacgacga tcgccgaaac
      961 caggagcttt tacagctact acgttgaatg ttccacggat tttgttcaat acaagagttg
     1021 gcaatgcttc accatctaca tcgtcagcaa taatcaacaa tggacgtgat tgttgcaaga
     1081 tttgttctaa caatggcaga atatcttgga tgttagagat ttttttgtct gtgatcaaga
     1141 tatatgggtt ttctagaaca gcttccattt tgtcgttatc tgttaccata tattgtgata
     1201 agtaaccacg atcaaactgc ataccttcta caacatctag ttctgtttcg atcccttttg
     1261 attcttcgat tgtgatgaca ccgtcgtttc caactttttc cattgcatca gcaatcaaat
     1321 gtccaacttt atcagaaccc gaagaaacag ctgctacttg tgcaatggct tcttttgaat
     1381 caacaacagt tgagatatta tgcaattctt cgactgctgc ttttgttgcc aattcgatcc
     1441 cgcgacgaat gcctagagga ttagcacctg cagttacgtt ttttaaacct tcgcgaacga
     1501 ttgcttgtgt cagaacagtt gctgtcgttg taccgtctcc tgcaatatca tttgtttttg
     1561 atgctacttc tgatacaagt tttgcaccca tgttttcaaa atgatcttct aattcgattt
     1621 cttttgcgat cgtcacacca tcatttgtga tcaatggaga gccgtaagat ttttctagta
     1681 cgacatttcg gcctttaggt cctaatgtta ctttcactgt atctgctaat ttatctaccc
     1741 cgcgtaacat agctgcgcgt gcgtcttctg caaatttcaa ttcttttgcc atgattgatt
     1801 cacctcattg attatttttg attatttttt tatcttccaa atttactcaa caatcgccat
     1861 gatatctttt cctgcgacga tcagatattc ttttccttcg tattttactt ctgtaccggc
     1921 gtatttttcg aacataactt ggtcgcctgc ttttacagcg gcaggaactt tttcaccgtt
     1981 ttctaacaaa cggccttcac cgactgcaac aactgtccca gtttggggtt tctctttagc
     2041 agcagaagct aaaacgattc ctccgacagt tttttcttct tcttttgcga cttcgataat
     2101 gacgcgatca cctaatggtt ttaacacgat aaatccctcc gataactctt tttttaatct
     2161 gttagcactc ttaaccttcg agtgctaacc tacattttta ataatactca ttctccctct
     2221 tttttgcaag cttttaaatc aaagaaaaga aattttattc tgacagctgc ttattgaaat
     2281 tatgatataa ttgaaatcaa tcgatataag cttgccttgc atgcttgtta tcccttaaaa
     2341 ggaggaatta catgtcttta aaaaaatata gcttcctcag cattttttgc tatggtctaa
     2401 tttttatctc acccttgatc ctagcttcga taggacttgt aaaatctacg tctgaattga
     2461 tcacagcaac tgcagtcatg tatatactag gcgccgttgt cctagctatt ttttatttta
     2521 agcaacgtga acccttagcg atcgaatcag cggtaaaaaa agcttctgct ccaaagattg
     2581 ttatttatgg attggttgga atctttgtcg cattgattct gcaaagcatc gctgtcatgt
     2641 tagaatctgt attgtttggc aaagcaaccc cttccgagaa tacacaaaat atcatccaga
     2701 tgatcatgga agcacccgct tttattttgg cgacaactgt tgctggaccc atcatggaag
     2761 agttcgtatt ccgccgcagt atactgggga tcatcagtcg atattcaaat ttttgggtcg
     2821 gagcagtgat cagctctttg ctgtttgcgt ttgctcataa tgatggtcat ctgctaatct
     2881 actttttcct aggtttcttc ttcagcctag agtacaaagc aactggtcgg atctggacat
     2941 cgatgatcac acacgtagga atgaacacat tagttgttct tgttcaatta gctgtccaaa
     3001 aagggctagt ataaacatga ctataaatac tcctgtcatc ctagattggt gattttccca
     3061 atctaaagcg acaggagtat ttttgttttt tagtcgttca tcttttctgc tgctccatca
     3121 ttttttgtct gtctttcctt tttgcagttt gcagaatcta tttttttcaa aaatgggaaa
     3181 taaatgaaaa aactaattga caacacgata ccttgtaaaa tcgctgcagg cactccgcct
     3241 actaagaaac ccgagaggat cggcggtgtt gtccaaggga cttggactgc tgtaaataaa
     3301 ggaacgaatc caaaatataa agcggaatag gtaatcaatc ctgaaacgac cggtgcgaaa
     3361 ataaaaggaa cagccatcat cgggttcatc acgatcggtg tggaaaaaat aattgattca
     3421 ttgatgttga aaaaggctgg taaaagagca agatttccta attctctgta tttcttcgat
     3481 ttcgcaaaaa ccgtcataaa aaatacagcc cctaacgtca atccagcacc cgtaaccgtc
     3541 atgaactgat cgagaaattg ctgagtaacg atatgtccgc catttgccac cgtcaactct
     3601 tgtcctgagg ctaagatttc tgcatttgct aatgaattcg cttgcaagac gggactaacc
     3661 aacccattga ccatcgcaga tccatgaaga ccaaataacc ataaaaacgg aacaaaaaaa
     3721 cctaataaaa gcacaccaaa caacgtgtct atgaacgatt gaatgggaag ctggacatat
     3781 gtccatactc cttgaacaac agtcattctg aacaaatgtt gaaaaaccgc tgaaccgata
     3841 agcgtaaaag tgactataac aatgattgga atcaatgaag taaaagactg ccaaacattt
     3901 tcaggtatct tctctctttg cttccaccct ttcgcaagtc ttactaaacg tgcatagccc
     3961 catccgatga tgagcccagt tgtaactgct cctagtatcc cttcagcacc tgtccagaca
     4021 gtatctatct gagtatttag tggctgaata agcagtagat aagcagacaa ggctgttatg
     4081 ccgactggta agcgatcgat gtcttcttct tttgcataag cgtatgcaat ggcaaaaacg
     4141 atccaaaagg ctatcacatc aaatgtagcc tggtatactt gtgtaaaata catagaaaat
     4201 cctgttttgt ctatccactg agcgatgtaa tccacaggaa aatgagcaaa taagaaaaac
     4261 agcgagccaa caataattat tgggatcgtg taagtcattc cattacgcaa agcagttaag
     4321 ggtctaagat tcacaacaga tgttatcatt ggtaaaaagg cacgatctaa ccactctttc
     4381 atttcttatc tccttcaact tctattttta tcaatcagtt ttcttaattt aactataccc
     4441 aatgatcacg aaccatgaaa ggaatgcgca ctgagctttt caaaaatcgg attctttgac
     4501 gaatattcag caactgctta caatggacag gaataaaaaa ggaggttttt tatgaaaatt
     4561 gcactcattg gagctactgg ccatgcaggt tcatttattt tagacgaagc attgtccaga
     4621 gatttagacg tgacggcaat cgtgcgtcat ccagaaaaat tgccatcaga tgttccgttt
     4681 atacaaaagg atttatttga gttgacaact actgatcttg catcttttga tgttgtcgtt
     4741 gatgctttca acgctcccaa aggaaaagaa gaaatgcatc aaacttcttt gtcccatttg
     4801 attgagatgc ttgaaggaac cgatacacgc ttaattgtcg ttgggggtgc ctcttcgtta
     4861 ttcttagata cagaaaagac gactcgtatg attgaccaag tacctaaaaa tgcacccttc
     4921 tatccaacag ctttcaatat gtacgaagga ttgttaaaat tgaaacaatc taaaaatcta
     4981 cattggactt atatcagtcc agcttcttta tttgatccac atggcaaacg tacaggtact
     5041 tatttgttaa gtgatgatta tttacaaaga aacgctgcta gcgacagtac gatcagcatg
     5101 gcagattacg cgattgcatt agtagatgag atactggatg agaaacatga aaagcagcac
     5161 atc
//
LOCUS       DAZKNM010000160         5132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000160 DAZKNM010000000
VERSION     DAZKNM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.163"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1276
                     /locus_tag="ACG8M9_002378"
     CDS             134..1276
                     /locus_tag="ACG8M9_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0379092.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEETETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPTLSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHLTPYSQRQTIEQDSAID
                     SGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNKSKPEIT
                     PGEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQ
                     GIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTF
                     ASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1402..2481)
                     /locus_tag="ACG8M9_002379"
     CDS             complement(1402..2481)
                     /locus_tag="ACG8M9_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0379093.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2590..3030)
                     /locus_tag="ACG8M9_002380"
     CDS             complement(2590..3030)
                     /locus_tag="ACG8M9_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0379094.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3087..3497)
                     /locus_tag="ACG8M9_002381"
     CDS             complement(3087..3497)
                     /locus_tag="ACG8M9_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0379095.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3678..4112)
                     /locus_tag="ACG8M9_002382"
     CDS             complement(3678..4112)
                     /locus_tag="ACG8M9_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0379096.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4212..5060)
                     /locus_tag="ACG8M9_002383"
     CDS             complement(4212..5060)
                     /locus_tag="ACG8M9_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0379097.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1875 a          981 c          850 g         1426 t
ORIGIN      
        1 tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt tagacaaaaa
       61 aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag
      121 gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt
      181 atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca aatggacaga
      241 aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc
      301 aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc
      361 gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg atcccacttt
      421 gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt
      481 caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga
      541 tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa ttgagcaaga
      601 ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac cagaaaatga
      661 aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac cggaaataga
      721 acctggagaa gaacccgata cagaaactca accagagcca gacaataaat caaaacctga
      781 aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
      841 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaactaga
      901 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
      961 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
     1021 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
     1081 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctag
     1141 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
     1201 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
     1261 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
     1321 tatccagaaa acgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
     1381 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
     1441 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
     1501 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
     1561 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
     1621 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
     1681 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
     1741 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
     1801 caacgtgttt aaaagagcga taacaaattg agcttctaat accaaaccaa atgtattgac
     1861 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
     1921 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
     1981 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
     2041 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
     2101 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg actggatcat
     2161 atcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
     2221 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
     2281 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
     2341 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
     2401 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
     2461 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gaaagagcaa
     2521 aaagaaaaac cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat
     2581 ctagcagttt cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag
     2641 atgttgcagt gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg
     2701 aataccttct gcttgttttt gcaacgcagc accttgagga gtcagcgtaa catttacgat
     2761 tctttcatca cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag
     2821 taagggagtc agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt
     2881 ctttttcttt tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg
     2941 cttgaggagg gaagtgtagt gtctaatgat ttccttagag gcggcatata aaggaaaaca
     3001 tacttgatta actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt
     3061 atcatagcgg atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata
     3121 tttccagctg tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc
     3181 gtttcttcca gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc
     3241 gtttcacttt ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt
     3301 ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac
     3361 aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg
     3421 aatgtacgat cgggattatg aacttctcca gagcgtccgc ctgtgttgat cattttcgta
     3481 gaatacattt ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac
     3541 acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca
     3601 tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaaatatcag agacaaaaac
     3661 taatctcatc ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaactt
     3721 acttcacgat ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag
     3781 ttagtcacac caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt
     3841 aatgctttat tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt
     3901 ggatgttcag cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat
     3961 ctcgtgccat acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca
     4021 atcagttgta tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt
     4081 aatacaaaat ctatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac
     4141 agttactaga ttcttttatt ataccagtta aagaaaacgc attcaaaaaa atagcggatt
     4201 tacttaccaa ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg
     4261 ctgctgacaa tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa
     4321 aattcatgat cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct
     4381 tttgcctcta gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct
     4441 tccaaataat gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata
     4501 taagtcatga caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt
     4561 ccaaaaacaa acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa
     4621 gtattcaagt ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa
     4681 gagttgtttt tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca
     4741 gacaacaacc gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa
     4801 aacaagcttc cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt
     4861 accaacataa cgatattcaa cactaaaata actatagctc ggtcaaatcc taaaacagct
     4921 tccagtaata ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac
     4981 atattgatag cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata
     5041 aattgtctcg tttctttcat ttatccactt ccttaattca ttaataatac tcttatctta
     5101 ttataactta cttttcacct taagttaact tt
//
LOCUS       DAZKNM010000161         5106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000161 DAZKNM010000000
VERSION     DAZKNM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.164"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..1597
                     /locus_tag="ACG8M9_002384"
     CDS             80..1597
                     /locus_tag="ACG8M9_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0379098.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1569..2858
                     /locus_tag="ACG8M9_002385"
     CDS             1569..2858
                     /locus_tag="ACG8M9_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0379099.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3033..3929
                     /locus_tag="ACG8M9_002386"
     CDS             3033..3929
                     /locus_tag="ACG8M9_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0379100.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3943..4806
                     /locus_tag="ACG8M9_002387"
     CDS             3943..4806
                     /locus_tag="ACG8M9_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0379101.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4812..>5106
                     /locus_tag="ACG8M9_002388"
     CDS             4812..>5106
                     /locus_tag="ACG8M9_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0379102.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLS"
BASE COUNT         1604 a          799 c         1063 g         1640 t
ORIGIN      
        1 aagtaagttt aacatttata ttattgacaa cgctttcaac gaggtgtatt attcatgtat
       61 aaattaaagg aggtcagtta tggaagccac aatgttgatt agtagtgtca agcgtgtagg
      121 caaagttgat ccgcggttgt acagttcatt tattgaacat atgggacgag cagtatatga
      181 agggatttat cagccagatc atccatcagc taataaaagt ggatttcgcc aagacgtcat
      241 tcacttggta aaagaattga acattcccat gattagatat cctggtggaa attttgtgtc
      301 aggatttcgt tgggaagaca gtattgggcc catagcaaat agacctagaa ggttggattt
      361 agcttggcgg acaattgaaa cgaatgaagt tgggattcat gaattttata actggtgtca
      421 gcatgtgggg gccgaaatca atatggctgt caatctgggt actcgtggag tagatgcagc
      481 tcgtaattta gtcgaatatt gtaattttcc taaaggaact tattggagtg atttaaggcg
      541 ttcgaatggt caagagagcc cttttggaat taagacatgg tgtttaggga atgaaatgga
      601 cggtccttgg cagattggac ataaaacagc ggaagaatat ggacggttag cagaggaaac
      661 ggctaaggcg atgaaactcg tagatgattc aattgaggtg gttttatgcg gaagttcgaa
      721 tcggcaaatg cctacatttg gtgattggga gttaacagtt cttgatcaag cgtacgatca
      781 aatagattat ttgtctctgc atcaatatta tggcaaccaa aaaaatgatc tttctcatta
      841 tcttgcacgt tccttagaca tggatcggtt catttctgaa gtgacagcga tgtgcgatac
      901 agtcaaggcg aagaaacata gtaagaagca gatcaacctg tcctttgatg aatggaatat
      961 ttggtatcat tcagcggaac aagatgagca agcagcgcct tggcagattg ctcctccttt
     1021 actagaagat aactataatt ttgaagatgc attactatta ggctgtctat taatcacttt
     1081 attgaaacat gcagatagag taaagattgc ttgtcttgct caattggtaa acgttattgc
     1141 accaatcagg acagaaacaa acggagaagc ttggagacag acgatctatt atccatttat
     1201 gcaagctgct gtgtatggac aaggagaggt gttaagccca caaattcgtt cagcaaacta
     1261 tgcgacggaa gattttgaag aagtgccgta tttagaaagt atagccgtaa taaatgacaa
     1321 agaacttgtc atatttgcag ttaaccgtgg agaagaagaa atgaatctaa ctgttcactt
     1381 gtctgaatta tcattagagg gagtgattga tttttctgaa atgagtggat ttgatacaaa
     1441 agcaactaat cttcctaaaa gtgaacaagt aaaaccaagt cattcaacaa atgtaaaagc
     1501 tgaagatggg aaaatcaatt gtttattgaa accgctatct tggaatgtgg tcagatgtcg
     1561 tttatcagat gaaggaggaa caaaaaatga aaggtaaaaa atggttaatt ggctctttgt
     1621 tggtagctgc aacaggtatt ttaggagctt gcggtggcgg aagttcagca gaatctgatt
     1681 ctgataaaga agtgactttg actttttgga atgggtttac tgcatctgat ggtgaaatat
     1741 tgcaagaaat cgttaatgaa tttaataaaa caaatgataa aaacatcaaa attgaaatgg
     1801 atatcatgac ttgggccaat ttgaatgaaa aacttcctac agcgatttct tctaaaaaag
     1861 cacctgactt tattgcatta aattatccag attttgcaca gtatgtagaa aacggagcgg
     1921 tacaaccatt agacgatttc tgggaatatg atggagtaga taaatctgat tttaaagaaa
     1981 cggctattga attaggtgaa gtagaaggta aacagtattt tgtccccatg caggtacaag
     2041 gcatgtatat gtattggaat aatgatttgt ttaaacaagc tggattagat actgaacaac
     2101 cacctactac ttgggaagaa ttgacagaaa tggcaccaaa attaactgat agtagtaaaa
     2161 atgtatcagg ttttgtcttc aataaggatg gaactgcacc gttatataac tggatgttag
     2221 caaatggcgg taaattagtg aacgatgatt atactaagtc tgaatttgcg tctcctgaga
     2281 accttaaaac gttaaaagcg atccaaaaaa tgatccatgt agacaagact ggtccagaat
     2341 cgatttctgg cgccgaaatg gacaatttaa tgaatgctgg tcaactagcc attgaaatca
     2401 atgggccatg gttaaataac ggattgaaag caaacgagat tgattataat gtgactacct
     2461 taccacaagg gagtgattca gacaaaaaaa cagctatttt agatggcgta ggatatgcca
     2521 ttccggcaag tacagataat aataaaaaag aagctgtcta tgagtttttg aaatattgga
     2581 atacaactga aattggtaaa aaatggagta tcgaaaatgg ttttcctgcc tacttggatt
     2641 cagtagcaaa agatgaagaa gtaaaaaatg atcctattgt atctgaatta tcgaaacaaa
     2701 tggactatgc agagccatat ttaccaggtt ttacccaaat ctctgcgatc aataacgata
     2761 tcatcaatcc actgattgaa aaattattag caggagaaga tcctgagaca ctcatgaacg
     2821 atgcagatga tgccatcaat caattactgc aaaaatagta cagaacttta tacaggatag
     2881 actgttggca agatacagat atctgaatga tcgacctagt atttgcagat aggtgttatt
     2941 gcaaaaatca cagcgattgt tcggatatct tattcagatt cgtttaaacc aagattgtat
     3001 attttatcaa aatgaaagat gaggtggact atatgaacgg attcaccaaa tattggaatc
     3061 gtcctgatcg tgctgcgtat ttgtttttaa ctccatcttt tttagtttta ttattattta
     3121 ctgtcatacc gcttttagga acatttgcta ttagttttac agatttgaat attttcttta
     3181 catcaagaaa ttttgtaagt tttgaaaatt tcacacgtat atttagtgat gaacgagcag
     3241 ttaattcgtt gattcattct ttatatttca ccgtactcga aacgccaaca caaattatcg
     3301 ttggtttatt agcagcagta gcattatcta aaaataccag attcaatcgt ttttgtcgtt
     3361 cgacatttta cattccagtt atttgttcat tgacgtcaat tgcgattatt ttttctatgt
     3421 tgcttgatcc gaatgtaggg gcgattcctt acttgttttc acaagtcggc ttgccgatcc
     3481 ctcaattttt tcgcgatcca acccttgcta tgccgacggt agccatcatg actgtttgga
     3541 agaattttgg tgttactatg acgattctct tgactgctat tcagggaatc tctccctctt
     3601 tgtatgaatc tgctcaaatg gatggtgcaa caaatcgaca gcagtttttt aacattacgc
     3661 tacctcagat cgttccctcc ttaggttttt gtattttaac gaatctgatt ggttcgatgc
     3721 aagtgtttga ccaagtatat gttgcaaccg ctggtggccc acaatttaag acagaaacag
     3781 ctgttcaata tatttacagt cgaggattta cagcacctta tgaattgggc tatgcatcag
     3841 cactgtcttc ggttttgttt atcattgttg caactatagc tatctccact aatgtgtaca
     3901 tgtcacggaa agaaaaacaa atgaaatagg agggaaacct gtatggcacc agaaagctcg
     3961 attacagtta ctgtgaatag tcgaaaagca gttagtggta aaaaaatcat tcgaacgatt
     4021 agtatgctag ttatattgtt ggttattgcg tttccttttt tgtggctgat tatttcttct
     4081 tttaaacacg aaaaagacat tatctcattt ccgcctagaa tatttgcaga tagctatacc
     4141 ctagataact atattaaggt ttggacaacg atccctttat tagattacat caaaaataca
     4201 gtgatttttg ctggagggac agtgatcact tctgtattct ttgattctct tgcggggtat
     4261 gcatttgctc gtatgcgttt caaaggaaaa tcagttttgt tctatttcgt tttgctgaca
     4321 atgatgatcc cattccaagt cttcatgatt cctctattta ttgaagtcaa tttgcttggc
     4381 atgcttgata cttatgcagg tttgatcatt cctcgtatga caactgcatt tggtatcttc
     4441 atgatgcgtt ccttttttat tactttacca gattctttgg aagaggcagc acgcattgat
     4501 ggattaagtg agtttaatat ttttttgaaa atcatgttgc cattgagcaa acctactctt
     4561 ttatcattag ggattttcac gctgatgaat agttggaatg acttgcttta tccattgata
     4621 ttgacaagta gttcaaaaat gagaacttta ccagcagggt tagctttatt tacagggcag
     4681 aacatttctt tttatggacc tgtaatggct ggaacggtta tttcaatgct gccattacta
     4741 gtggtttata tttttgcaca gaaatatttt gttcaaggaa cagctatgtc tggaatgaaa
     4801 gagtgaggaa gatgaaaata gttgagacta aattttatgg acagttgatt gtggtttctg
     4861 attacatctt attaggtatt ctctgggtaa tcgtgtgttt gcctgttatt actatcgtgc
     4921 ctgctacatg tgctgttttt tatgtaatgg cgcaatggaa aggtgaggga tcaggaaata
     4981 tcatttattt tttctttcaa ggaataaagc aacattttgg taagaacttg ttccttagtc
     5041 tgttaacgat cattatttat tttcttgtta atcaattatt agaagaacag acaatcgtac
     5101 tttcaa
//
LOCUS       DAZKNM010000162         5105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000162 DAZKNM010000000
VERSION     DAZKNM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.165"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..477)
                     /locus_tag="ACG8M9_002389"
     CDS             complement(19..477)
                     /locus_tag="ACG8M9_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0379103.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(490..1821)
                     /locus_tag="ACG8M9_002390"
     CDS             complement(490..1821)
                     /locus_tag="ACG8M9_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0379104.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1832..3274)
                     /locus_tag="ACG8M9_002391"
     CDS             complement(1832..3274)
                     /locus_tag="ACG8M9_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0379105.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3261..4025)
                     /locus_tag="ACG8M9_002392"
     CDS             complement(3261..4025)
                     /locus_tag="ACG8M9_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0379106.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4129..5004)
                     /locus_tag="ACG8M9_002393"
     CDS             complement(4129..5004)
                     /locus_tag="ACG8M9_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0379107.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1539 a         1084 c          750 g         1732 t
ORIGIN      
        1 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
       61 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      121 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      181 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      241 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      301 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      361 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      421 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      481 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      541 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      601 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      661 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      721 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      781 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      841 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
      901 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
      961 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1021 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1081 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1141 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1201 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1261 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1321 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1381 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1441 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1501 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1561 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1621 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1681 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1741 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1801 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1861 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     1921 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     1981 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2041 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2101 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2161 tgtttctctt attgatcaaa taataaaaag taacgtaata caaagcaaaa aagaaaattc
     2221 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2281 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2341 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2401 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2461 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2521 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2581 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2641 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2701 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2761 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2821 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     2881 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     2941 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3001 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3061 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3121 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3181 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3241 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3301 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3361 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3421 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3481 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3541 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3601 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3661 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3721 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3781 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3841 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     3901 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     3961 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4021 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4081 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4141 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4201 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4261 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4321 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4381 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4441 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4501 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4561 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4621 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4681 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4741 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4801 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4861 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     4921 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     4981 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5041 aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5101 taatt
//
LOCUS       DAZKNM010000163         5063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000163 DAZKNM010000000
VERSION     DAZKNM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.166"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1995
                     /locus_tag="ACG8M9_002394"
     CDS             61..1995
                     /locus_tag="ACG8M9_002394"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0379108.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2452..2868
                     /locus_tag="ACG8M9_002395"
     CDS             2452..2868
                     /locus_tag="ACG8M9_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0379109.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2882..3448
                     /locus_tag="ACG8M9_002396"
     CDS             2882..3448
                     /locus_tag="ACG8M9_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0379110.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3471..4457
                     /locus_tag="ACG8M9_002397"
     CDS             3471..4457
                     /locus_tag="ACG8M9_002397"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0379111.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4612..>5063)
                     /gene="scm"
                     /locus_tag="ACG8M9_002398"
     CDS             complement(4612..>5063)
                     /gene="scm"
                     /locus_tag="ACG8M9_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0379112.1"
                     /translation="STDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTS
                     ASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKT
                     SNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1536 a          935 c         1114 g         1478 t
ORIGIN      
        1 caaactaaaa gaattatcat atattcatta tatagatagt ctataaggag cggatgatca
       61 atggcaaagc aaaatattgt cgttgtagga gcaggatatg ccggtgtttc agctacaaaa
      121 tacttggcaa agaaactgaa aaaagaagat gtagcaatta ctctgatcga tcgtcattcg
      181 taccatacta tgatgaccga gttgcatgaa gtagctggcg gacgtgtaga accagaagca
      241 atccaatacg atctgcagca tttgtttgct cgtcaaaaga atgtacaact tgtcacagac
      301 actgtaatcg gtatcgataa agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct
      361 tttgatcagc tgatccttgg aatgggcgga gagccgaatg actttggtac acctggtgtc
      421 aaagcacacg gattcaccct ttggtcattt gaagattctg taaggattcg ggaacatatt
      481 gagaagacag tagcaaaagc tgcattggaa ccagatgatg aactgcgtaa agccatgctg
      541 actttcgttg tctgtggttc tggatttacc ggtattgaaa tgattgggga actgatcgac
      601 tggaaaaatc gcctagccaa agattataaa ttagatccaa acgaaatcac actgatggtc
      661 gtagaggcaa tgccgacgat cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa
      721 cgctatttgc gtaagcacaa cgtggaatta ttattgaatg caccaatcgt agaagtggct
      781 gctgatcata tcaaattgaa agacggacgc aacattccga ctcacacact gatttggaca
      841 gctggtgtca aaggaacttc tgacgctgct gactttggat tagaatcagc acgcggacag
      901 cggttgatcg ccaatgaata tatgcaggca aaaggctacg aagataaaaa catctatatc
      961 attggtgatt tagtctttta tgaagaattc ccaggaacac cgacaccgca gatcgttcaa
     1021 gctgctgaac aaacaggaca cacagccgca gcgaatatcg tggcttcgat caaaggcgga
     1081 gaaaagcata aatttaaagg aaactatcaa ggatttatgg tttctattgg tgccaaatgg
     1141 ggggtagcta acctatttga taagatccat ttaagcggat ttttagctat cgtaatgaaa
     1201 catatcgtga atttgaaata cttctttgat attcgttctg gttattatat gttccaatat
     1261 ttaatgcatg agtttttcca tatcaaagat gatcgcaacg tgactcgagg acattcttct
     1321 cgttacggca atgtcttatg gagtgtgcca cttcgtattt tctatggttt agtttggttc
     1381 gttgaagctt ccaaaaaaat cattgggaat ggtgaagtgt tcaaaccaag tacttggttt
     1441 ggtgaaggat catggttcac tggaaacagt gtgttccctt ggacatggga atacaatgta
     1501 acagatgttg ccagtggtgc ttctgctgct ggtgatgcaa ccacagctgc cagcggagca
     1561 ggggcaagtg ccggagaagc agcgactcaa gctgcacatt ttggtttgag ttatgcatac
     1621 ggtgaagaac caatggctgt gattgagaag tcaccagact ggttcacaaa gatcatgcaa
     1681 gtgatcatgc caaacaatga tgtagcaatg ttcttccaaa aattcatggt attctttgaa
     1741 attgccttgg cattagcatt gattgctgga ttattcacat ggctatgcag tgcgacaacg
     1801 attgtattgg tgatcacgtt ctgcttatca ggcatgtttt actgggtaaa catttggttc
     1861 atttttgttg ctttcgcatt gatgaacggt tccggacgtg cgattgggtt ggatcgctgg
     1921 gtaattccat ggctacaacg tacactaggt aattggtggt acggtagacc aagttcccag
     1981 tacggcaaga aataaataga taaagaacgt tatttgaatg caagatttca atagaaaggg
     2041 gctgagacaa accgtcacag cctccttttt atgtaaaatt aaaggaataa gatgcccgag
     2101 ataagcctcc atgagcaaga cacaggtgtc acaacctctg ttacaacctc ttgttataaa
     2161 cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag
     2221 taattttcgt aaattccttc ttatttcttg aagttgccca cttctgtcac aacctcttgt
     2281 tataaacggt gttccacctg ctgcttctcg gtaataagcc caaagaatta gaaaacatgg
     2341 aaagctgttt tctattcttc gttcttatta ctcaaagcaa gacacaggtg tcacaacctc
     2401 ttgtttttat gctaaaattg ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt
     2461 aaagaatttg ctgaaaaaag tctgctcaaa ccttgggatg tttttattat tttgcttttg
     2521 actgttcttt cttttttacc agtcatgatt tttggtcttc agcaaaacac cgcaacagca
     2581 gaaaaagaag cggtactgcg tgtggatggc acagaaatca aaacattccc tttgatggct
     2641 ggagaagaga gcaacacata tacttacaaa gatgaacacg gagattataa cttgatcgag
     2701 atagatggcg atcgagtacg gataaaagaa gcagattgcg atgaccagat ctgcgtacgt
     2761 cgaggctggg caacgaaaaa tggcgaaacc atcgtctgtt tgccacataa attagtgatc
     2821 gaagtccgtt caacagatgg aggtgacgaa gacagtctga tttattagac cttcacacac
     2881 catgagtaaa ttacaaaaaa tgatttatat ttcgctactt gttgcacaag gagtgatcat
     2941 tggtttattg gaaaatatga tcccttatcc tttttctttt gcgcctggtg caaaactagg
     3001 cttagccaat ctaatcacga tcgttgccat ttttaccatg aaaaaacgag acagcttctt
     3061 actactttgg ctacgactat tcctgactac tttgctaggc ggaacgattt ccacattcct
     3121 ttatagtatg agtggtgctt tgcttagtta ttttggaatg ttattggtca agcaattagg
     3181 accaaaacgt gtcagtatca tcggaattag tgcggccggc ggtttcatgc ataatgtcgg
     3241 acagcttttg atggcttctt tcattgcaca atcatggaca gtcatgttgt acttgccagt
     3301 cctttcagtt ttagggattt tatcagggat tgcaatcggg atcgcagcga attatctttt
     3361 acgacatgtg cagacacttc aacgattcca aaaagattat gagcagggaa ccaaacaaaa
     3421 atggagccca atgtttacca aaaaataatc gtcacacaag gagttttttt atgaagctac
     3481 atccaatgtg ggaaaactat cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga
     3541 tggaagaaac tgtccgcctg aaaaacaaac ctgtcgaaca agcaatcaaa gaaacgatcc
     3601 atgccggcgg aaagctgttg cgtcctgcct atcagctatt gttctcacaa tttggaccag
     3661 agcaagatcg agacaaagcc atcgctttgg cagcttctat cgaaatgctc catacggcca
     3721 cactgatcca tgatgatatc gttgatgaag cggatctgcg acgtggtcag cctaatatcc
     3781 gtagtcagtt tggtaatgtc gtttctgtct atgcaggaga ctatttattt gtctgctgtt
     3841 ttaaactatt gtctgattac tccacttcct tgaaaagtct ccaattgaat tctcgcagta
     3901 tggaaaaagt attgaatggc gaattagggc agatggatga tcgctataat tatgaacttt
     3961 ctgtaaaaga atatttggaa aatatttcag gtaagacagc tgaactgttc caattaagtt
     4021 gttctgttgg cgcttttgaa agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata
     4081 tcggactttc tatcgggatg gcctttcaga ttatcgatga tattttagat tatacaaaag
     4141 agagccagga aatcggcaaa cctgtactag aagacatgcg tcaaggggtc tattctttac
     4201 cgttgatcta ctctttgcaa aaaaacaaac caaagctact accttattta gagaagaaag
     4261 cggcaatgac agaagaagat gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg
     4321 tggaagaagc taggaagtta gcggcctctt acacagaaaa ggctttgaag gaaataaaga
     4381 aattacctgc cacctctctt cgaacaaaag agaatctctt ttcccttacc caactgattt
     4441 tggatcgcaa agactgaata gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa
     4501 tctacgtgtt ttttgacaaa ttgaattgta gttcttgtat ttatgataaa aagcaaaaat
     4561 aaagagcaac gtccgttagc gacgttgctc ttttttatct tgaatatttt tttacctttt
     4621 cgatttctta taactcataa aagtgattct taaaccgatt acaagtaata taacgcctgc
     4681 gagtgttatc caattattcg attgacttcc agttttagga agtgctcttt ttgatttgtt
     4741 tccattgtca ttgcttgttt tattagttaa gttaaagttc gtcctgttat ccgaacttgt
     4801 ttttgagaag gagacacttg catgacgtgt atcgcttgtg gtagacgacg tgtctgtact
     4861 atcgcttgaa gttgatgaat ctgagctatc acttgtggta gaggatgtat ctgtactgtc
     4921 acttgaagct gacgtgtccg tgctatcact tgtagtggta gatgaatctg agctatcact
     4981 tgtggtagag gtagtatctg tactgtcact tgaagctgac gtgtccgtgc tatcacttgt
     5041 tgtggtagat gtatccgtac tgt
//
LOCUS       DAZKNM010000164         5044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000164 DAZKNM010000000
VERSION     DAZKNM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.167"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..991
                     /gene="rnhC"
                     /locus_tag="ACG8M9_002399"
     CDS             74..991
                     /gene="rnhC"
                     /locus_tag="ACG8M9_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0379113.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(1369..2280)
                     /locus_tag="ACG8M9_002400"
     CDS             complement(1369..2280)
                     /locus_tag="ACG8M9_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0379114.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(2681..3259)
                     /locus_tag="ACG8M9_002401"
     CDS             complement(2681..3259)
                     /locus_tag="ACG8M9_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0379115.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(3279..3947)
                     /locus_tag="ACG8M9_002402"
     CDS             complement(3279..3947)
                     /locus_tag="ACG8M9_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379116.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(3962..5032)
                     /locus_tag="ACG8M9_002403"
     CDS             complement(3962..5032)
                     /locus_tag="ACG8M9_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0379117.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT         1515 a         1052 c          838 g         1639 t
ORIGIN      
        1 tctcgcaatc tcttagaaaa cgaaaagtcg tgagttgcgg tatagtttta tgtataaaaa
       61 cgaggagaat tttatgagca acgtcattat caaagcaaca aacgaccaac ttagtcaaat
      121 gaaagcatac tatcgctctt atttgttaaa taaacagatt ccttatacta gctttgctgc
      181 aaaaaaagac gggacgacca ttaccgctta tacatccgga aaagtgatgt ttcaaggtac
      241 gaatgcagca atagaagctg ataaatgggg ggaggcacag gataaacaag ccaagccagc
      301 aaaaacagcc tcttctttgc caaaaaatat cagtcaatta tccgttttag gtagtgatga
      361 ggtcggaaat ggcagctatt tcggtccgct aacagtatgt gctgcttatg tgaaacgtga
      421 tcagcttgca gaattacgta aacttggtgt tagagattca aaagagttaa aagatagcca
      481 aatcatccag cttgctgaca tcttaaaaaa aaccatcccc tataaactgc ttgtcttatc
      541 tccagaaaaa tacaatgaga tccaaccaaa atacaatgcc gttcggatga aagtcgcttt
      601 gcacaatcaa gcgatccatt tgctgctaaa aaaaatcgag ccagaaaaac cagaagcgat
      661 tttgatcgat cagtttacgc cggaagcaaa ttttaaaaaa tatgcgcgtt tagaaaaaaa
      721 tcaattacaa gaaaaactgt acttcgttac taaaggagaa caatatcatt tagctgttgc
      781 tgctgcttct atcatcagtc gtgccagctt tttggaagag ttagacaaag catcagctga
      841 agctggaatc acccttccgt caggagctgg aacgaaatct gaccaagtag cggcaaaact
      901 attggagaaa ggcggcatgc ctatgcttga aaaatatgcc aaacttcatt ttgccaatac
      961 agaaaaagct ttgaagctac taaaaaaata atggaactgt gacaaaagtc atgtcacagt
     1021 cccttgttcc gaataaacgg tgttcaatca tctgatcctc ggtattagct caacaaacgg
     1081 caaaatatga ggaactattt ttccgtttgt ttctctaata ctcagaacaa aacgttgatt
     1141 tcacaacctt tttcttaaac ggtgttctca tgccaactcc tcggtcacaa gtcacaatag
     1201 tcatcaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca
     1261 ggctcacgac ctcttcctta aacggtgttt aaaagcttgc atctgcgata ataagcccgg
     1321 ccaaacgaaa aatatgagga gctatttttg tttggctggg cttatttatt actgaaaata
     1381 agtggtcaat gcttgtgtca aacttgcagc aatctgctgc tgataactgt ctgttactaa
     1441 ttcctttaaa tcactatcat tattcatata acccatctcg attaaaatag aaggttgaag
     1501 attttctcga agaacttgat agttcccagt ggagattcct cttgaatcca gaggcagatc
     1561 agtgaaggag tcagcaattg tctgtgctaa tgtttcctct tgtccatagt aataatatgt
     1621 cgtgattcca gttgcttcat tcgcatattc cgtagagtct gcgtgtaaac tgataaagac
     1681 atctgccttt ttttcttcac tgtagtcgca tatatctcca agctgtacta attcatctgt
     1741 agtatgggtc aaataaaccg ttgctccagc atcctctagc gcctttttta cttttatggc
     1801 tgtacttaat gttatatctt tttcttctgt ttgttcatca ttgcttaatg ccccagtatc
     1861 atttccgcca tgcccaggat ctaatacaat cactgcatca gatagctctt ccggttttgt
     1921 ttgtatattc tttgtgactt tggttccatc ttcgagggct aaatatccag tatagccatc
     1981 atttgtcttg atctgataag cattttttgt ttcagaaaga aactccgcca gctctccgcc
     2041 gtttatagtt gcaagtgggg tagttagcga gtcatctgta tatagtgtac ggtcattttt
     2101 tccgattttg attaatgtct cttgttctgt atcatcaaat gattcctttg tctttgtaac
     2161 cgtactgttc gtactgctga ccgtttgtcc aactatcttt ttttgaccaa aaacatctat
     2221 ttgaaaacag ataataatga taatgattgc aaatacaaaa gctccaatta tttttttcat
     2281 tcttgactct cacctcgtag atccagacta agggccaaaa cttaaaatca gcttaagtaa
     2341 gcaaaaaagg ggctgtaaaa gatataaccg tagaaaataa cattactgct aaaatgattt
     2401 ttttcatttt tggcatatcc tgaaatctcc ttttgactat tcgtggtgat tcatctaccc
     2461 aataaatagt ttccttcatt ctacaaacaa ttaatttctt cgttattaaa ttaaaacact
     2521 tcctggaaca ccgacataat aaataaaaat ataccctgat aatcatgaga gaataacaaa
     2581 tttgtattta tgaaaaacta cctatataaa gaagcaagat ataacgatct tcgtcatatc
     2641 ttgcttcttc acctaactat tttctcccta tataacagct ttacgcttct cctaatacac
     2701 cattcaaata taaatcgatc aattgcgcca catttagcgg ttcttccaca ggtcttttcg
     2761 accgagtgat gtaaggtgct aacactaaga aaaaatgcct tgctgcgatt tcttttgaaa
     2821 tcccatttct tagcacagat tggtttgctg aaaagatttc ttcaaaaggt tcacgataat
     2881 ttttcttcca tagaaagaaa agatgatctc ttgtttcctt tgaaagattg ctttcaagat
     2941 cgtgaagcat cagaaacaaa tcgaccagat gtgtctcaat caaaaactgc gccatttttg
     3001 tcaaggttac ttgaaatccc gtttcgcttg tttgaacgat ttctctcaaa gcttttccaa
     3061 catcttttaa gtggttttct aaaactgccg tatatagttt ttctttatca ccgaaataat
     3121 gatagagatt gggctgggtg atcccaactt tttgtgcgat ttctcttgtt gaagtgccga
     3181 gatatccctt ggacataaac aagtcagtcg cttctttcaa aattttttgc tgtgtatctt
     3241 tgttgtatcg atggaccatt tttccacctg ctttttggtt attctttttc tttcaataca
     3301 ccatcttcca tcataaatat tctatcgcat ttgtcaatca gccgtgtatc gtgagtaacc
     3361 atgatcgttg ctttattttt gtcttttgat tcttttgcca agatatcgac tacttctcgt
     3421 gccttttctg aatccaaact agcagttggt tcatctgcta gaataatgga cggatcattg
     3481 tacaatgcac gagcgattgc gaccctttgc cgttctccac cagatagttc ttctggatac
     3541 ttgttgatca acttatccac gccaagctgc tcaaataatt cgtttgcttc tttttgacga
     3601 tgttcatgtt taatcttatc tactaataat aactgctttt ttactgtcag aaaaggaacc
     3661 agattggaag cttgcaaaat aaagccaatt tctttaaaac gtagtttaac gcgttcgctt
     3721 tctttttgat tactgaattc ttgatcattg ataaatacag agccactgct aggcgtctgc
     3781 aatccgcctg ccaaagtcaa aaaagtactt ttacctgaac cagaaggacc gatgattgca
     3841 acaaattccc ctttatcgat actcatggtt gtttctttca atgctttaac tacttggtcg
     3901 ccatcttgga aacttttttc tacttcttta aattctattg ctttcatgtt cttcctcctt
     3961 cttatccaat tgccttcaac ggatcgattt ttacgattgt ccgaacagaa aatagtgcgc
     4021 ctaacacagc tactactaac atcaacaagc taatgacgcc aaaaaacagc caattgcttt
     4081 ggaatggaac cgcatcaggt agaacaaggg atgttcccac tgttcctaat agaccaagaa
     4141 gaataccaac aaatgataaa atgaatgttt gtgccactac agaacgcgca ataaatcctc
     4201 ctgtgatccc ttgtgctttc attacaccaa agatattaat cttttgcatc gtcagtacat
     4261 aaatgaaaat cccgatcaca atagccgcaa taacgatcaa aaatccgatc ataaatccaa
     4321 aagtcaatac ttgcgcatta tatccaggta attcattgat gaatttagaa attttgatct
     4381 gttctaaatc ttcaggtaaa ttactgattt tacctctgac aactattgcg ttaatccgtg
     4441 cattttcact tgtatcttct ttttcgaagc ggatttcttg gtaagcattg atggttgtat
     4501 agagaactgg tgagacattg aatttggcat gatccgtaaa gccagttagt ttaaacgtct
     4561 tgtcactacc agataactta actgtatctc ctatggctag accatattct tctttcaaac
     4621 taatatcgcc tactgcctca tcatcgttat caaaagcttt tccttcaaca agattgggca
     4681 tgatgaattg atttttatcg ataccgaaga agctaacatt gatttttcct tcttctgtac
     4741 tgtctttact agtcacgact ccaggtgttt gggcaagata cgccactttc ccaccttcga
     4801 cttcttctgc tatcttctta gtaatcatgg acatccctag atttgaattt gcatctttcg
     4861 aaagcacaat cgaatctgcc tcccacttgt cgactgctgt tcgattatct tgagctaagc
     4921 cataagctaa cccagtcaaa aaaaatacca ggtaggcgat caaaaacatc acccctgcaa
     4981 ctaacgcata acgtaacttt gaatgcataa tttcatttaa tgctaaaaac atttaactcc
     5041 tcct
//
LOCUS       DAZKNM010000165         5042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000165 DAZKNM010000000
VERSION     DAZKNM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.168"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8M9_002404"
     CDS             complement(<1..182)
                     /locus_tag="ACG8M9_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0379118.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8M9_002405"
     CDS             517..951
                     /locus_tag="ACG8M9_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0379119.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8M9_002406"
     CDS             974..1294
                     /locus_tag="ACG8M9_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379120.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8M9_002407"
     CDS             1435..1626
                     /locus_tag="ACG8M9_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379121.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8M9_002408"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8M9_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0379122.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8M9_002409"
     CDS             2112..2375
                     /locus_tag="ACG8M9_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379123.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8M9_002410"
     CDS             2399..2587
                     /locus_tag="ACG8M9_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379124.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8M9_002411"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8M9_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379125.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8M9_002412"
     CDS             3070..3441
                     /locus_tag="ACG8M9_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0379126.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8M9_002413"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8M9_002413"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8M9_002414"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8M9_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379127.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8M9_002415"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8M9_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0379128.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1757 a          784 c          986 g         1515 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 ga
//
LOCUS       DAZKNM010000166         5000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000166 DAZKNM010000000
VERSION     DAZKNM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.169"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8M9_002416"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8M9_002416"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0379129.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8M9_002417"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8M9_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379130.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8M9_002418"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8M9_002418"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0379131.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8M9_002419"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8M9_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379132.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8M9_002420"
     tRNA            3917..4000
                     /locus_tag="ACG8M9_002420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5000)
                     /locus_tag="ACG8M9_002421"
                     /pseudo
     CDS             complement(4240..5000)
                     /locus_tag="ACG8M9_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1537 a         1029 c          800 g         1634 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata
//
LOCUS       DAZKNM010000167         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000167 DAZKNM010000000
VERSION     DAZKNM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.170"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8M9_002422"
     CDS             85..972
                     /locus_tag="ACG8M9_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0379133.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8M9_002423"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8M9_002423"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0379134.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8M9_002424"
     CDS             2274..2720
                     /locus_tag="ACG8M9_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0379135.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8M9_002425"
     CDS             2993..4345
                     /locus_tag="ACG8M9_002425"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0379136.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8M9_002426"
     CDS             4516..4803
                     /locus_tag="ACG8M9_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0379137.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKNM010000168         4929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000168 DAZKNM010000000
VERSION     DAZKNM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.171"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..936)
                     /locus_tag="ACG8M9_002427"
     CDS             complement(25..936)
                     /locus_tag="ACG8M9_002427"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0379138.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1313..1951)
                     /locus_tag="ACG8M9_002428"
     CDS             complement(1313..1951)
                     /locus_tag="ACG8M9_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379139.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1955..3937)
                     /locus_tag="ACG8M9_002429"
                     /pseudo
     CDS             complement(1955..3937)
                     /locus_tag="ACG8M9_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4117..4782
                     /locus_tag="ACG8M9_002430"
     CDS             4117..4782
                     /locus_tag="ACG8M9_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379140.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
BASE COUNT         1569 a          911 c          764 g         1685 t
ORIGIN      
        1 gttcatattt tttatttata ccgcttattt actacttgct gtactagaat ctgaagtgaa
       61 tgtactcaaa acattttcaa agtctttgtc tttgattttt acattagcgt ctttcaatac
      121 ttcgccaatt actttattct ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc
      181 tttcaattct tttttgtatt tatccatgtc gttgcctttg ttttggtttt tcaccatttt
      241 aaccacataa tattccgtag tataagttga agcattggta gaagtgatca catcagaaac
      301 ttgaccatct ttcaacttaa aggctgcttc tttgacttct gccggaaccg ttgtagtagt
      361 ggaatcaaat ttcacttttc cgccatcttc ttttgtcgta gtatcagttg acttgtcttt
      421 ggctaattta gagaaatcgt ctcccttatc agcagctttc ttcacatctt ttgcatcatc
      481 ttcacttgct acttggataa tttgagcttc cacttctgga tggaaagatt cccaagccgt
      541 ttttaaatct tcatcggtca atttgatatg tttctttaga ccttcttgaa aggctagact
      601 ttgtttgata ctgtctttga atgtttgttc tgtgtaacct gcagatttca attgtgaatc
      661 aaatgaatca cccaattgtt tctttgtttg atcatattgg gaatcgattt ctttggttga
      721 tacgtcatct ccatatttat cttcaaatac ttgataaacg atcaactgtt gcacagcgga
      781 atctgctcct tgcatttttt tcaagctatc cattacttct tgttcgctga ttgtaccgcc
      841 tttataacta acggctgttt tttcaccggc attcgtacag cctgacaata agccagtgcc
      901 tagtacgatg gcaagtgcta aaaatctttt tttcatttat caattctcct acttttcgtt
      961 ttttgcgaat tgctaaaaac aaaggtgttc aagatgaaaa tcatcaagaa cacctttact
     1021 aatctgctcg atacgtcctg aatagatata aggctacaca atccagaaag ttcgctcagc
     1081 agaataaatg tcaagtagca catcacacct taatatacta cttcttttgt taccgttttt
     1141 caagtctatc ttgcgttccg tatttaacgt aactaattct tagaacaaat gttatcgatt
     1201 ttgtttttct tcttccgctg ttgggatatt caaatcagac acttttatgt tcgattttcc
     1261 agacattttt ttgttgtttt tattagaaaa agtttgtttt ttcatttgtt ttctaaagca
     1321 tgatcttctt gtttcctatt ttggtcagat atggatcatg cgtgaccacg ataactaacg
     1381 taccttggga agcctgttgc gataagattt caacgacttg ctccttattt cgtttatcta
     1441 atgatccagt tggttcatcg gctaaaatga ttttgctatt cttcaataag actctcgcac
     1501 aggcgattct ttgcttctca ccaccagata atgtgtagat aggcgtactt agcttcttgt
     1561 ttatatttac ttttttcaat gcctcatact ttgctgttcg cttatctgaa acatttaaat
     1621 tcgaaaactt caatccaata tctaaattaa aatcgattgt ttcatattca atcaacccaa
     1681 aatcttgaaa aagaaaagag agggcatttc tttttatcaa gcgcttctct cttccagtta
     1741 aatgttttgt ttcttgatta aataaataaa attgccctga actgaattga tcattcaatc
     1801 ctaatatatt caataaagta cttttccctg ctccgctctc tccaactaca gtcacaaaat
     1861 ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga atgatttttt
     1921 tcgcaccttt tatcactatc aacggctcca attgttaacc tccattcatc agcaataact
     1981 gacttttctt ttctgtagac aaaataaaaa caactgaaag aataaaatca atgaagctaa
     2041 agaaaaaaat attaaaaaga tatttaggct ctctttctat aagaaaaagg aagattacac
     2101 ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc tttatacgtt tcaaagagcg
     2161 aataaccact tgaacgaaag atagcgattt tccgacaatt gtagacaaaa tatatgcata
     2221 acatgacata gctcaacaag aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt
     2281 aatgaaatga tctgcttaac actttgacag ctatctcttc agtctgagta tacggaaatg
     2341 ataactgcaa gttatcacgt aactgacttt cttcaatcag ctcaaataac ctttctttcg
     2401 acaaagagcc tatcttgatc tttaacggtg gatagatttc tccgctgaga atatttcttt
     2461 cccaatacgt actgtttttc aatgtcagta tttcaacaac cggataatgt tcgatccagg
     2521 ggatagaagg aataaatgta taaatgggtt gattgttttt tataaataga aatttcggtt
     2581 ctttttcact gatttcttga taatattctg ttatttgcgc caggtcattt tttaaatctg
     2641 tatgtctata tcgttcaggg atcaatataa cagggtgcgt ttctttttcg gttatttgga
     2701 tcaaccttcc agagacatca atcaaaggaa atttcttaag ataattcaga tttattttta
     2761 tccctctgcc aaactgttca ttttcttcat tcaagtaatc tgatacattg actaatatac
     2821 ttccgttttt attcaaagtt tcatataatt cctgatcggc tgctgatgca aaacgattgg
     2881 aataaataaa atccacgtgg tttttcccga tggttactgg ataaaataca tgatagtcct
     2941 caccagcgat ttcttttggt gtttcatggg aaacagctaa ctgtgaagaa gaaaaaacga
     3001 tagttgctaa ttcgacattg gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa
     3061 aaagaatgta ttggatatag ttgaacgctt ctcttaacga agcgattttt tcaactaata
     3121 aaagagaaat caaagagata ccctatattc cgctgaacaa gaaaacgtac atctggaaca
     3181 aataaagaaa ttgctcctta taatacataa aaataaaacc accaatgatt cctgcaatta
     3241 tgaatgtaat aatgattttc tttttcccca ataaacacca caatacttga tgtaatgaaa
     3301 agccattatt caacaataaa ctgatttctt tattcatcaa taagaaaaag gatgataaca
     3361 aaaggaataa aagaatcaat ccaatagtta aatacactcg ataattatat acgtctgtac
     3421 ttgaaataac cgaatacgaa tagctatttt caaagtcttc aaaaatatta gtggcataat
     3481 cagtgtgaaa tgcttgattg aatcgttcca caaatagtaa aatgaaccgt tcataggctt
     3541 ctttttctct cgtcatcaag aaaaattcac cttcaaaatc ttttattttg atcgattctt
     3601 caatggacgt tacgcgaaag gaggtattag aatagggtac cttctcgtga gaagatttga
     3661 catccccttg ataatacaat gtcatttcaa gctttggctg tatcttgaag ttccaagatg
     3721 tatctacttc attggattca tttactgttt ttttcatata agaaagctca gtttctgatg
     3781 agacattttc aaataccatg attagctgct catattcttc ttgcccaata aaatgttgtg
     3841 gcaatcgaat cggagtataa gtatctgttt gatggaaaaa ttcatttgtc cgatagagta
     3901 cctgtttatt tataataacc aaggcaaaac cactcatgat accggtcact atgattgtga
     3961 gtaaaactgt gaactttttt ttgattatca ccactctccc ttacttaaat gattctttga
     4021 taaaatcata ccgtttttca tcaaaaaaac gtatctatca aatgtatagg tcatgtagaa
     4081 taaatctata tactgtagaa tcgaaaggag aagaagatga acatcggaga aaaaatacga
     4141 acttaccgca gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat cggtgtttcc
     4201 gataaaacaa tctctagttg ggaaaattct cgtacaatgc cagaccttga aatgctgtca
     4261 cttttacatc agactttagg tctgccgatt gattttcctt ctattgaatc agctaccgaa
     4321 tcaatagaac caacattgcc ggatgaacct cttgttatca agaaatctta ttcatcaaaa
     4381 caacccttct cactcaaaaa gacactatct ttctttctgc tcgtcttttg tttggtctat
     4441 ctttttctta tcaattatca atataaaaat gaaacggttg atcgattcaa tccttttttg
     4501 aaaccacaaa tcggctatac cacactccct gtaggatacg tggaaaattc catcgaacat
     4561 tgggtatgta atgatatctt tgttcatggt acatggctgt cttttcacgc tggagaggta
     4621 gaaaaaggta agaatcatgc tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc
     4681 ctgctcactt gggaagccat tccctcagat taccaagaaa tcctcatcca agactatgac
     4741 gatgccttaa aaagaagccc tcaggagcca gataggcatt aagtaggaga tgaacgtaga
     4801 gatagaaaaa atcaatgcac atgatcttat caaaaaaaaa caggggtaca aattaattga
     4861 aaaagctttg agatgaaagt tcctaaataa aaaagttcag aatgataggt aaaaaaatga
     4921 ctaactatt
//
LOCUS       DAZKNM010000169         4844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000169 DAZKNM010000000
VERSION     DAZKNM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.172"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1680)
                     /locus_tag="ACG8M9_002431"
     CDS             complement(70..1680)
                     /locus_tag="ACG8M9_002431"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0379141.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1902..2147)
                     /locus_tag="ACG8M9_002432"
     CDS             complement(1902..2147)
                     /locus_tag="ACG8M9_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379142.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2873..4213)
                     /gene="glnA"
                     /locus_tag="ACG8M9_002433"
     CDS             complement(2873..4213)
                     /gene="glnA"
                     /locus_tag="ACG8M9_002433"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0379143.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4241..4633)
                     /locus_tag="ACG8M9_002434"
     CDS             complement(4241..4633)
                     /locus_tag="ACG8M9_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0379144.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1421 a         1044 c          796 g         1583 t
ORIGIN      
        1 gagaaaagtg acaagaaaat tgcttatccc cttctcttgc tacttttctc tttctatcaa
       61 gttcaaaaat tattttgttt cgtgatctgc taatgatgct ccgacaaatg ctttgattaa
      121 tttttgcggg cgatttggac gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa
      181 ttttttctct ggcagctcta cgatttctac taaacggtta tctggtgaga cacctgaaaa
      241 gactagccct tgttcttcaa acaattgacg atatttgtta ttgaactcat agcggtgacg
      301 gtgacgttct tgcacaacat cggctccatc atatgcttca gccgtcttcg ttcctttttt
      361 gattttacat gggtataatc caaggcgtaa agttccaccc aaattctcga tgttttcctg
      421 atcagccatt aaatcaataa tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc
      481 atcttctaat ccaacgacgt tacgtgcaaa ttcgacacaa gccatctgca taccaagaca
      541 aatacctaaa aacggaacgt cattttcgcg agcataacga atcgcttcga ttttcccttc
      601 tactccgcga tcaccaaatc caccaggaac caagattcca tctgcgtctt tcaaacgttc
      661 ttctacattt tctctcgtta attcttgtgc tttgatccaa tcgatctcga tgtctgaatt
      721 ataggcgaaa ccagaatgtt tcaatgcttc aactactgag atatatgcat ctggtaactc
      781 gacatattta ccgacaagcg caatccgtac ttttttcttc aagttcaaga cacgttcttc
      841 taattctttc cattctgtca tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac
      901 aatttgatcc attccttgtg cttgtaaaac taatgggatg gagtaaagtg tatctacatc
      961 tggcgactcg ataactgctt ctggttcgac atcacaaaat tgtgccaatt tgtttttcac
     1021 gctttgagaa acaggttgct ctgtacgtac caccaaaata ttcggttgga tacctaaacc
     1081 acgtagctct tttacactgt gctgcgttgg tttagttttc atttctccag cagctttcaa
     1141 gtaaggaatc aatgttgtat ggatatacat aacattatct gtaccgacat ctgctttcat
     1201 ttgacgaagt gcttcaagga atggcaaaga ttctatatcc cctactgttc cccctacttc
     1261 tgtaatgatc acatctgaat cagtcatttt agctgctcgc atgatttttt ctttgatctc
     1321 atttgtaata tgaggaataa cttgaacagt tgctcccaag tattcgccct tacgttcttt
     1381 acgcaaaact tcagaataga tttttcctgt cgtcacgttg gagtatttgt tcaaattgat
     1441 gtcaataaaa cgttcgtagt ggcccagatc caaatctgtt tcagcaccat catctgttac
     1501 gaatacttct ccgtgctgat atgggctcat cgtccccgga tccacgttga tgtatggatc
     1561 aaatttttgg atagttactt ttaagccgcg gtttttcaat aagcgtccta aagaagctgc
     1621 aacaatccct tttccgattg atgaaacgac accgccggtc acaaaaatat attttgtcat
     1681 aagttcaaaa actccttttt aatttatgtg caggaaaagt ctcagacaat aaacaaagct
     1741 ccctattctt caataagaat agggagccga aatttctgta aacttctgac cctctaaaag
     1801 ggtgcccaag gtgtataata caagctgact aagggaaagt caagtactca aatgaatact
     1861 atccgcttac aattttttct ttttcgatat cctttgagaa attatttctt aagtccttta
     1921 tgagtgcaaa aaaaatttaa cccacataat aagcttgata taaacatagc taacgttaaa
     1981 cctaagcaaa tcagaaaatc gttactgaaa aattttaagt gaaataatag ataaaaaaat
     2041 aaaacaaaca ttatgataga taatatccca cctaacaaag aactactttt tcctacttgc
     2101 agcaacagtt tatatagctt atcgtagaaa caatcttttt ctttcaccct tatttacctc
     2161 ctttacgtaa aaaacgtagt ccacagttct cggatttttg gcatatcttt tctattaaat
     2221 aatatcaaaa acaaaacaat tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa
     2281 gtgccttgta gggattcatt gaaggtaacg aatcacataa gtaaaaaata aggagatgcg
     2341 acaaaagtcg tgcctcctta tttctaaata aacggtgttc tcctgccaac tcctcggtca
     2401 caagtcacaa tatccatcaa acatgagaag ctgtttttga aattatttct tgtgattcgg
     2461 agttaaatgg taggctcaca acctctggat aaacggtatt caaaagccag tttctcggta
     2521 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttaatactc
     2581 gaaactaaac gtctttttca aaacctcttg gtaaacggtg ttcaaaagct tgcatctgcg
     2641 gtaataagtt cagccaattc aaaaacaaga aaagctgttt ttgcttggct attcttatta
     2701 cttgttgcag ggcagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc
     2761 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccgtta gtttctctaa
     2821 tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg ttttaataaa
     2881 gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc gcccattcga
     2941 gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca atcattacat
     3001 tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc tggatatgtg
     3061 cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct gctggcggtg
     3121 tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt gccaagtatg
     3181 ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg
     3241 gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca ggcgcttcat
     3301 aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct gtgtaggcac
     3361 gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt tccaattcac
     3421 cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg caatgcatac
     3481 cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc
     3541 gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca tctaccacat
     3601 ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca
     3661 cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt tcacctaaat
     3721 ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg atcccaccgt
     3781 cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa gaggtaaaac
     3841 caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg
     3901 gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca tgagcacttt
     3961 cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg ctttcttcaa
     4021 ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct aatactttgt
     4081 cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct gtgaacatca
     4141 atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca cctgttatct
     4201 gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag aattgtcttt
     4261 gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc aaaatctcat
     4321 cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt gcttcttgtt
     4381 ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat ccctctgaaa
     4441 gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata cgtcggtttc
     4501 cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt tgccgagcag
     4561 acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat cggcgcaatt
     4621 ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg taccaattaa
     4681 aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta ttttttataa
     4741 aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac cagatacctt
     4801 ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DAZKNM010000170         4747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000170 DAZKNM010000000
VERSION     DAZKNM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.176"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8M9_002435"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8M9_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0379145.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACG8M9_002436"
     CDS             complement(1164..1874)
                     /locus_tag="ACG8M9_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0379146.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2802)
                     /locus_tag="ACG8M9_002437"
     CDS             complement(1942..2802)
                     /locus_tag="ACG8M9_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0379147.1"
                     /translation="MFVAIWGKWSTRRIKMKVLGLGDNVVDKYENLGIMYPGGNSLNF
                     AVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGD
                     RIFLGSNSGGVTREYPIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYD
                     FSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIG
                     YDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVD
                     GSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACG8M9_002438"
     CDS             complement(2768..3736)
                     /locus_tag="ACG8M9_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0379148.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>4747
                     /locus_tag="ACG8M9_002439"
     CDS             4499..>4747
                     /locus_tag="ACG8M9_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0379149.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLE"
BASE COUNT         1460 a          980 c          721 g         1586 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggag
//
LOCUS       DAZKNM010000171         4744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000171 DAZKNM010000000
VERSION     DAZKNM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.177"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG8M9_002440"
     CDS             complement(136..1098)
                     /locus_tag="ACG8M9_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0379150.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG8M9_002441"
     CDS             complement(1100..2539)
                     /locus_tag="ACG8M9_002441"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0379151.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG8M9_002442"
     CDS             2724..4670
                     /locus_tag="ACG8M9_002442"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0379152.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1508 a          812 c          779 g         1645 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaat
//
LOCUS       DAZKNM010000172         4723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000172 DAZKNM010000000
VERSION     DAZKNM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.178"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8M9_002443"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8M9_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0379153.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8M9_002444"
     CDS             724..1878
                     /locus_tag="ACG8M9_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0379154.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8M9_002445"
     CDS             2093..3061
                     /locus_tag="ACG8M9_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0379155.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8M9_002446"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8M9_002446"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0379156.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8M9_002447"
     CDS             4091..4699
                     /locus_tag="ACG8M9_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0379157.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1457 a          907 c         1109 g         1250 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaa
//
LOCUS       DAZKNM010000173         4482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000173 DAZKNM010000000
VERSION     DAZKNM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.179"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..885)
                     /locus_tag="ACG8M9_002448"
     CDS             complement(<1..885)
                     /locus_tag="ACG8M9_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0379158.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKL"
     gene            complement(878..1558)
                     /locus_tag="ACG8M9_002449"
     CDS             complement(878..1558)
                     /locus_tag="ACG8M9_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0379159.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1559..2341)
                     /locus_tag="ACG8M9_002450"
     CDS             complement(1559..2341)
                     /locus_tag="ACG8M9_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0379160.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2334..3251)
                     /locus_tag="ACG8M9_002451"
     CDS             complement(2334..3251)
                     /locus_tag="ACG8M9_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0379161.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3415..3669)
                     /locus_tag="ACG8M9_002452"
     CDS             complement(3415..3669)
                     /locus_tag="ACG8M9_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379162.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1326 a          957 c          777 g         1422 t
ORIGIN      
        1 caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat
       61 tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg
      121 gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg
      181 tttgatagga ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt
      241 tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa
      301 catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat
      361 atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa
      421 agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa
      481 ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc
      541 ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa
      601 aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc
      661 ttgaaatgct tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag
      721 gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc
      781 agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa
      841 acttcccata aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc
      901 cccacaccgt atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa
      961 tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa
     1021 gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt
     1081 ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca
     1141 gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat
     1201 gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat
     1261 agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa
     1321 gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa
     1381 gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt
     1441 ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca
     1501 gtaaggaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct
     1561 acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta
     1621 ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg
     1681 tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg
     1741 cgaaaacacc gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga
     1801 cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca
     1861 gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa
     1921 cttcaccaaa tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca
     1981 cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat
     2041 tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa
     2101 agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa
     2161 tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg
     2221 tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg
     2281 agacccacat tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca
     2341 tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga
     2401 ataccatcca caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca
     2461 attccttcaa aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga
     2521 gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga
     2581 gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata
     2641 ccatagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg
     2701 atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga
     2761 tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt
     2821 gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta
     2881 ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc
     2941 gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca
     3001 atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc
     3061 gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga cttacccgct
     3121 ccattttccc caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct
     3181 aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt
     3241 ccaaaatcca tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa
     3301 agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa
     3361 caatcggaca atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt
     3421 tcctgattct ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt
     3481 tgcttctttc agatcaagct tgagtaattt taccagaccg gttataagga taactatagc
     3541 aagactccct cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc
     3601 aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat
     3661 cgttgacata agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata
     3721 aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt
     3781 tgggatttac aattagctac caaataagag gttatgacaa aagacttttc acaatccctt
     3841 cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa
     3901 acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac
     3961 aacctcagaa gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt
     4021 atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa
     4081 aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc
     4141 agcattgctt cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta
     4201 acgttttgag aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc
     4261 attgaataag tttgaaaaac cttgatttat aaaggttttc ccgtaaatat attttattat
     4321 atttgattaa atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa ggcagtatat
     4381 ttcgtatttt agattaaatt tttaggctca ttcattctca aatccattct attcagtcaa
     4441 taaaaatttt aaaaagactt tataaacaaa gatgttcaag cg
//
LOCUS       DAZKNM010000174         4428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000174 DAZKNM010000000
VERSION     DAZKNM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.180"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1402
                     /locus_tag="ACG8M9_002453"
     CDS             68..1402
                     /locus_tag="ACG8M9_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0379163.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1443..2354
                     /locus_tag="ACG8M9_002454"
     CDS             1443..2354
                     /locus_tag="ACG8M9_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0379164.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2416..3204
                     /locus_tag="ACG8M9_002455"
     CDS             2416..3204
                     /locus_tag="ACG8M9_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0379165.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            3201..3817
                     /locus_tag="ACG8M9_002456"
                     /pseudo
     CDS             3201..3817
                     /locus_tag="ACG8M9_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            3819..4337
                     /locus_tag="ACG8M9_002457"
     CDS             3819..4337
                     /locus_tag="ACG8M9_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0379166.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1325 a          758 c          900 g         1445 t
ORIGIN      
        1 tttcagaaca aaatgatatc gttatattaa aaccggcatc ggttacatta aaatttggag
       61 gtaaaatatg aaaaaattat tgtttacagg gttgctggca agttcagcat tgtttttatt
      121 aggaggatgc ggatcaagta atgcaagcga tgccagttca agtgcagcaa attcttctga
      181 aaaaacaagt gatggaagaa aaaatattga tttctggtcc ttttggggat ccggtgcacg
      241 aaaagaagct atcgaagaaa tcattgagga tttcaatcaa tcacaaaacg agatccatgt
      301 aaattataag tatcagccat ggggtgacat ctggactaaa tctttatctg caataacagc
      361 tggaaatgca cccgatgtta ttgtacagga cataaattcc gttgcccaaa gagcagaagc
      421 acagcaagca actaatctct ccaaatatgt agaaaaagac acttctaagg atttttatcc
      481 gcagctttgg gatacagtag aatatgaagg tgatccttat gcgatcccat tcaatacaga
      541 tacacaagtc attttttata ataaaaagat atttaaagaa gctggaatca gtgaacaaga
      601 tctaccgaca acttgggatg aattagaaga aacagcacgt aaactagata aaaaagaagg
      661 aaaagaattt acacgcatcg gtttttatcc attatggaat ttaggggctg atgtatgggc
      721 gctaaacgca gataacggga ctagttggtt tgacaaagat gaaaaagtca agattgatac
      781 taaaaataaa gtggaagcct tggagtggat ccaagactgg caagattatt atgggaaaaa
      841 tacgatcaat aaattagaag ccgaatttgg gtctggtgta tctgatccat ttatttccgg
      901 attagtagcc atgcgtgccc aaaatatcaa ttattactct agtttgatgg aaaatgcacc
      961 ggatgatttt gattttggcg tgatgcagct cccagaaaaa gaagaaggtt caggccattg
     1021 gtcgtggggt ggcggattcg tcttggaagt accatacgga gcaaaagatc ctgaggcctc
     1081 ttatgaattc atcaaatatc tttcttctcc agaagtacaa gaaaaattcg gcgaaaaaag
     1141 ttttgatatc atggcgagca agacagccaa tgagaattta gtgaataatg acaacctaac
     1201 ggataaagga aaaatgatct atcaaatggc ggacgaaaac tttaaaaata cggtgatcac
     1261 accagttcct ttagatgctc cggattacac gacattagtg aatgaacaga ttgaccagat
     1321 tcttttagga acaaaatcac caaaagaagg attggcagat gcacaaaaag cagtagaaaa
     1381 cttagtcaag caaaatcaat aatccatttt tgactgtcta ggtacagagg aggcaagaaa
     1441 atttgaagcg tttatctatc cgaaagaaaa aagaagcaag atgggcctat ttatttatct
     1501 cgccttttat tatcgggttc ttggtattta tgctagggcc tatgcttttt tcagttattg
     1561 gtagtttcac cgattataat ttgacatcca agatgaattt cattggtcta gcaaatttca
     1621 aaagaatgtt cgttcacgat gatttatttt ggaaatcgtt atacaacacc gtttatttcg
     1681 tcatcttcaa tgtaccgctg acgacgattt tcagtgtgtt tttagccaca gtactcaatc
     1741 aaaaaatcaa aggaatcagt gtattccgta cagctttcta tttaccggca gtcttatcag
     1801 gagtagcagt ctacatctta tggatgcagc tgctttctcc atctgcagga ttgatcaaca
     1861 catttctggg ctggttccat atccctggac cagcttggct gtttgatcca caatggacga
     1921 aacctgcgtt gattttgatg aaaatatgga gttctggagg agcaatgcta ctttacttag
     1981 ctacattaca aaatgttcct agatctttat atgaatcagc agaaatcgat ggagcaggcg
     2041 tttggggaaa gttcaaaagt attactttgc cattaatcac tcctattatt ttttacgatg
     2101 tgatcacctc tacgatcggt tctttccaaa tttttcaaga agcttatgtc atgacgaaaa
     2161 atggaacagg cggacctgca aattctttgc ttttttataa tctgcatatg tggaacaagg
     2221 cgttcgtagc ttttgatatg ggttatgcga tggctatgtc aatgatcctg tttctgatcg
     2281 tgttggttct gacgtttgtc aatttgaagc ttgcacctaa gtgggtctat tattctggag
     2341 gtgacggcaa atgatggatt atttcaaaaa gaccagtgtg aatcggcaaa caaaaaaata
     2401 ctaagttatg tcctcatgac agtaatagga atcattttga tcattccatt gctttggatg
     2461 gtgtttactt cattgaaacc aatggaagaa atcgttcgtt atcctccgac ctttttccca
     2521 gaaaaaatcg tatgggaaaa ctatctggat acgattgccg cctttccttt ttggcgctat
     2581 gcaagaaata cattattcat cactgtgcta gtagtgatcg gtaatgtcct ttcaaattct
     2641 tttattgctt atggatttgc gaagcttgat tttcctggga aaaagctgat gtttgctttg
     2701 gttctttcta cgatgatgat cccggggttt gtaacgatga tccctcaata tgtattattc
     2761 tccaagatcg gctgggtagg tacttatttg cccctgatcg ttccatcgtt cttcggcaat
     2821 gcgttcaata tatttctgat gcgtcaattt tacttatcga tcaataatga attgatcgaa
     2881 gctgctgaaa tcgacggagc gaatcatttg tatatttgga gtcatttgat gcttccattg
     2941 acgaaacctg ccttgattac cattgcgatc aattccttca acgcagcttg gaatgatttc
     3001 ttaggtcctc tgttatatat ccaagatcaa gaaaaataca cgttacagat tggcttgcaa
     3061 gtttttcaga accaagcaac gacacaatgg aattatctga tggcaggagc aacacttgtc
     3121 ttgatcccga cgattttgct gttctttttt gcacaacgtt atttcattga agggatggat
     3181 ttgacaggag gttcaaaagg atgatgaatc gatcgattgg taaaaaagcc aatgattatt
     3241 tcttagaaat cattgaatgg attccacgtc ttatccattt acagctacta tggttcctgt
     3301 gcgtcttgcc tgttttcaca ttaggaactg caagtcgaac agttctgtat atgatttatc
     3361 attacaaaaa aatgaagaga aaaagctcgg ttcactattc tggaaaaagt ttcgtgagaa
     3421 cttctctctt tatggaaagc aagatagttt agcaagtgtt tattttcttc ttctattgat
     3481 cgattatcag attttccgct attggggtgg gggaattggc tatacattga tgtatactac
     3541 gttgtttttg cttctattaa gcgcgcttct ctttagttac aaaatcttgc ttcaactgga
     3601 acaaaaagaa gcttcttgga ttactgcatt tttcttgttt tttaatgatt tcaaaagcgc
     3661 gcttttctat ttggcaggca cgttgatcct tcttgtcgca ttctggtttg caggaccaat
     3721 ctattttgct ttattaggtt tcagtttctt gttttatttt caagtcctgc tttttatcca
     3781 tcgtacgcaa gaaaaggttc ttaaaaagga ggaataacat gttgcgtttg attgctaaaa
     3841 tcgatgatag tattatcaac aagttcgcca agcaacggaa aacggatagt cgaaaaatca
     3901 attttttgaa atttatcagt aatatctcta atggtggttt tatttggttg ctccttgctg
     3961 ctgttttagt cttgatacct gatactagag aatatggtct agccattctt atcggcagtt
     4021 tgatctattt ttttgttggt ttgcttttta tcaaaaaaac gacaagacgg aaaagacctt
     4081 atgaaaaaaa tcgtcggctt gtcctttaca caagtaaacc gaagacttct tcttttcctt
     4141 caggacatac agcgctagcc tttttatcag tagctgcgtt gttcgaatta agtccagttg
     4201 caggaatatt agccttttta tttgcttgtt tgatcgctta ttcgaggatt tttttacttt
     4261 tacattatcc tactgacatt ttaggaggag ctattttagg aagtgttatt ggcatcctat
     4321 gcgctgccgt tatctgaatt aattgtacga ataaagcatg agtgcgtaaa tgcgcatttg
     4381 tgctttattt ttttgtttcg aggaaagcgt agttgtttat tcatttgt
//
LOCUS       DAZKNM010000175         4380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000175 DAZKNM010000000
VERSION     DAZKNM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.181"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            269..997
                     /locus_tag="ACG8M9_002458"
     CDS             269..997
                     /locus_tag="ACG8M9_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0379167.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1142..3169
                     /locus_tag="ACG8M9_002459"
     CDS             1142..3169
                     /locus_tag="ACG8M9_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0379168.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3277..4374
                     /locus_tag="ACG8M9_002460"
     CDS             3277..4374
                     /locus_tag="ACG8M9_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0379169.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1534 a          714 c          911 g         1221 t
ORIGIN      
        1 tcgtcaagtc acattttgat agataggagg gggacttacg aattatttta aaggctatga
       61 cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg
      121 gctgataatc atttataact aatcgataca taggaccaaa tataggtcta tgttctttta
      181 tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt
      241 agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat
      301 gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa
      361 ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc
      421 tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc
      481 tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa
      541 aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag
      601 aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag
      661 tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct
      721 gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt
      781 gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc
      841 aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga
      901 tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa
      961 attagtttgg acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa
     1021 ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc
     1081 taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat
     1141 attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc
     1201 gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga
     1261 aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag
     1321 tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc
     1381 ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa
     1441 tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga
     1501 aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc
     1561 aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa
     1621 tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc
     1681 tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg
     1741 ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag
     1801 tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt
     1861 cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac
     1921 ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc
     1981 ggagcaaaga agtcttactt atgatttgga aggtgtcggt cgctcttctc ggattatacc
     2041 tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg
     2101 tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac
     2161 agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga
     2221 taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat
     2281 gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg
     2341 aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac
     2401 agattatttt attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt
     2461 aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga
     2521 gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta
     2581 ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat
     2641 aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc
     2701 agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa
     2761 aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc
     2821 cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg
     2881 ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt
     2941 tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc
     3001 cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga
     3061 ttctacagct catcgtggaa tcagaataaa tattcctgta gaaaaccaaa gaatcggaac
     3121 ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa
     3181 gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc
     3241 acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg
     3301 agaccaaaac aacaattatt tataacactc ctagttattt taggggcact ttttaacaat
     3361 atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa
     3421 atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta
     3481 aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc
     3541 gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg
     3601 agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct
     3661 aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta
     3721 ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag
     3781 gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag
     3841 acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat
     3901 gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta
     3961 gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa
     4021 gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa
     4081 ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat
     4141 ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt
     4201 gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg
     4261 attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt
     4321 ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa
//
LOCUS       DAZKNM010000176         4374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000176 DAZKNM010000000
VERSION     DAZKNM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.182"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..818
                     /locus_tag="ACG8M9_002461"
     CDS             <1..818
                     /locus_tag="ACG8M9_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0379170.1"
                     /translation="TYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYA
                     MKKGYVEKNPIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWL
                     YLTGMRSGEAIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREI
                     DLPKKAIAIYNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHI
                     FRHTHISKLAELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(950..1297)
                     /gene="rplS"
                     /locus_tag="ACG8M9_002462"
     CDS             complement(950..1297)
                     /gene="rplS"
                     /locus_tag="ACG8M9_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0379171.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(1433..2194)
                     /gene="trmD"
                     /locus_tag="ACG8M9_002463"
     CDS             complement(1433..2194)
                     /gene="trmD"
                     /locus_tag="ACG8M9_002463"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0379172.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            complement(2184..2705)
                     /gene="rimM"
                     /locus_tag="ACG8M9_002464"
     CDS             complement(2184..2705)
                     /gene="rimM"
                     /locus_tag="ACG8M9_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0379173.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            complement(2875..3666)
                     /locus_tag="ACG8M9_002465"
     CDS             complement(2875..3666)
                     /locus_tag="ACG8M9_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379174.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            complement(3791..4042)
                     /locus_tag="ACG8M9_002466"
     CDS             complement(3791..4042)
                     /locus_tag="ACG8M9_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0379175.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            complement(4054..4329)
                     /gene="rpsP"
                     /locus_tag="ACG8M9_002467"
     CDS             complement(4054..4329)
                     /gene="rpsP"
                     /locus_tag="ACG8M9_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0379176.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
BASE COUNT         1292 a          852 c          774 g         1456 t
ORIGIN      
        1 agacttacat cgtttctggt attaatacaa tagatttgaa taatattttt gaagacatga
       61 tatataaaga tgacttgtca aataagtacg tcagtgtaat taaatccaaa ttgaatcttc
      121 ttttttcata tgctatgaaa aaaggttatg tagaaaaaaa tcctatcgat gaagtagtta
      181 ttgattataa aagagagtca aaaacgataa aaattaaaga taaattttta gaagatgatg
      241 aatataatag actagtagat ttcacaactt cacacaataa aagatattct ctccttttcc
      301 agtggctata tttgactggg atgaggtctg gtgaagcaat cgcattaagt aaagatgatg
      361 tacacattac caataataat gcatcagtag ttataaatgg gacgatgatg tatagagaac
      421 gttcaatagc tgatatgaaa aaatctgatt ctacaaaaac agctgccgga atgcgagaaa
      481 ttgatttacc aaaaaaagcg atagctattt acaatgagct tctagaatta aatccaaatg
      541 gtcaatttct atttcaaaca acgaaaggaa ctcctttcca actaacagca attaacacct
      601 atttgagaag tcataaagct gacatgaaga ttgataaaaa acttagttca catattttta
      661 gacataccca tatttcaaaa ttagcggaac taggaactcc tctgtatgcc attcaggatc
      721 gcgttggtca cgaaaatagt gatatcactg aaaaaattta tttgcatgtg acaaaaggag
      781 taaaagaaaa attgaaagaa gatatagaaa aactgtaact tgttgcccct tttttgcccc
      841 tttttattaa tcaaaaatgt accaaataga aatcgttaat ataaaaaaac agataataaa
      901 aaaagaaccc ttgaatacca agggttcttt ctctgtctga taacgatgat taacgacgga
      961 tttctttgat acgagcagct tttccgtgta atgcacgtag gtagtacaat tttgcacgac
     1021 gtactttacc gtagcgaact acttcgattt ttgcaacacg tggtgtatgc aatgggaatg
     1081 tacgttcaac gcccactccg ttagaaactt tacgtactgt ataagtttcg ctgattccag
     1141 caccgcggcg tttgataaca acaccttcaa ataactggat acgttcacga gtaccttcaa
     1201 caacttttgc gtgaacacgc acagtgtcac cagggcggaa cgctgggatg tcagaacgta
     1261 attgttcttt tgttaattct tcgattaatg gattcatggt tttcttctcc ttattccaaa
     1321 tattcttatg agcttttctc acagcggaat atcgtaatag ttaagtgctt ttgcactcac
     1381 cataaaatac taacatatta tcaacctact gtaaagaaga ttttagaaaa agtcacttgg
     1441 cttcatctgc tgttttttct tgttcttctt tcttgatttc ttctaaaaat ttcgtcattt
     1501 gaggtgtcca ttcaagtgtt tccaaaagat caggccgacg ttgataggtc cttcttagtg
     1561 attcttttaa ttgccattct tcaatcaatt tatgatttcc atttgtcaat acttctggta
     1621 ctttcatccc tttatattct gccgggcgag tatattgggg atgttccaat aaaccggttg
     1681 aataagaatc cgtttgagct gaagtctgat tccctaacac atctggcaaa agccgaactg
     1741 ttgcatcgat catgaccatc gcacctaatt ctcccccagt aaggacataa tctcctaagg
     1801 aaacttcatc tgtcactaat gtgcggatac gttcatcata tccctcataa tgaccacaaa
     1861 tgaatactaa gtgttcttct tgtgaaaatt cttctgccat tttctgatcg aatcgtttac
     1921 cagctgggtc aagaagaatt actctttttt tgacatccgg tgcagctttt tcaatggctt
     1981 ggatgttatc atagatgggc tggactttga gcagcatacc tgctccaccg ccgtatggat
     2041 aatcatctac tgtctgatgt ttgttatctg aataatcgcg gaagttagag atattgatat
     2101 ctaataatcc tttatctaca gccttaccga tgatggattc ccccatagga ccttcaaaca
     2161 ttcttggaaa taaagtcaat acatcaatct tcatcgtcaa tcagtccttc tggaatttct
     2221 acacggacca ctccagcttc taaatcgact tctttgacga cagaagcgat atatggaagc
     2281 aatatatcag cttttttcgg acgttgaacg acccaaacat cattggcacc tggtgacaag
     2341 atttctttta ctttacctaa ctcagttcca tcttctaata catgcagacc gatgatctca
     2401 tggtaataga attcattttc agctagatct acagagttgt cttttgacac ttttaatatg
     2461 ccatcccgat atttttccac atcattgata gaaggatgtc cttcaaaact caataagtca
     2521 aaatttttat gtttgcgatg acttttgact gttaattcca caggtgcttt tccgtcttgg
     2581 aatagtgtta gcaccgttcc ttttttgtaa cgttcttctg ggaaatccgt tgttgagata
     2641 acacggactt cgcctttgat accttgtgtg tttacgattt ttcctacatt caagtattca
     2701 gtcaacttat atccccattt cttttttgtc tgttttattt tagcatgtgt gaagaggcaa
     2761 ttctagtttt cttttaaggt attaagccaa aattatgcaa aaatgcggcg tgctattttt
     2821 gattacttcc tgtgacttgg agttaaatga tgggctcaga tcagcgcgct gatttcacaa
     2881 cctcttcata gaaactgcca tcttcaacgc agacatagga tcttgatgag tgatcgtata
     2941 ggcatttttc ggttcttgcc agcttacttc tacggctcga ttcgtcgtat cacccatatc
     3001 aacagtaatt ttaccaaatt gttcaggtgc tggtagactg ttttcttcca aataagctac
     3061 gatttcttta gctaatagta agggatcttg gccttctata ttattcccac ggattcgaat
     3121 acgccagttt ccttcttgcc attcaagaaa acttgaacct gctgcacctt gctgataccc
     3181 tgtgattcga gaacctaagt ccacttgctg accatttgtg tcgatctcaa atggctgcaa
     3241 aacttggatt gtttcttgac tagaagcaaa cccatattga tatagatagg ttgcaatagg
     3301 cgtttcttga ttcaattcgt cagcattcaa cccgagggga cgatctgttc tgtaatataa
     3361 aatagaataa ccttgctttg tatgtgtcgc tgcgatattg agatagcctg aagagactgg
     3421 tacatctaga ggcatgggaa catttggctg ttgttctttc aactggtcta aagcagtctt
     3481 tttctcatta tttgaggtag ccattgtttg ttcagtggtg gaagccgttt tggtactact
     3541 tttttctgga ctggtaacag tttcagatat actgcttgat agatgtgtat tgtctgattc
     3601 ttgagtaacc gaactacaag cgcttaacaa tagagcaaat ggaataaata acattttctt
     3661 tctcattggc tgttcactcc ctcctcctat aaggatacgc tttcttgctt gttttgccaa
     3721 ataatcattt cttcaattaa taaaaaaaac aaaaaagctg cactcgaaaa ttcgagtaca
     3781 gcacgtttat ttacttttcc ttatcatcga tgatattcaa acgcactttt ttcggtgcat
     3841 tgatccgaac accgtaaacg attgtgcgga ttgctttagc aacacgccct tgttttccaa
     3901 tgatacggcc aatatcttca ggagccactg ttagattgta ctcgtaaaag tctttcgact
     3961 ctacgacgtc taacttgact tgatcaggat aagtgactag cggacgaacg attgttaaga
     4021 ctaagtcgct taaatctttc atatccggtc accttatttc tttgagaatt tagcttcgtg
     4081 gtgtttttgc ataacgcctt cttttgaaag gatgttacgt acagtgtcag aaggttgtgc
     4141 accttttgat aaccagtcaa gaactaagtc ttcttttaaa actacttctg cagggtcttt
     4201 caaaggattg taagtaccta cagtttcgat gaaacgtcca tcacgaggag aacgtgaatc
     4261 agctacgacg atacggtaaa acggactctt tttagaaccc atacgtttta aacggatttt
     4321 aactgccatt atttatttcc acctccattg ttttattcac aagatgtagt gtaa
//
LOCUS       DAZKNM010000177         4325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000177 DAZKNM010000000
VERSION     DAZKNM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.183"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8M9_002468"
     CDS             complement(<1..149)
                     /locus_tag="ACG8M9_002468"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0379177.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8M9_002469"
     CDS             complement(593..1714)
                     /locus_tag="ACG8M9_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0379178.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8M9_002470"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8M9_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0379179.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8M9_002471"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8M9_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0379180.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
BASE COUNT         1426 a          778 c          814 g         1307 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtc
//
LOCUS       DAZKNM010000178         4310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000178 DAZKNM010000000
VERSION     DAZKNM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.184"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      12..178
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            326..883
                     /locus_tag="ACG8M9_002472"
                     /pseudo
     CDS             326..883
                     /locus_tag="ACG8M9_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            941..2980
                     /locus_tag="ACG8M9_002473"
     CDS             941..2980
                     /locus_tag="ACG8M9_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0379181.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3089..4240)
                     /locus_tag="ACG8M9_002474"
     CDS             complement(3089..4240)
                     /locus_tag="ACG8M9_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0379182.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1557 a          794 c          853 g         1106 t
ORIGIN      
        1 atagaatata gtaataacct tgttaggtga ggctcctata caaacatagg ctgttgccca
       61 gaaacgtcga aagtcgccaa tgggtaaaac agaaactgtt ggattaaggc tttttccaat
      121 agctaagaat gttttcttta cgttgtatag tgctaaaact cggacgatga gatgatgcta
      181 ggtcagaatt cttgttcgaa aaggtttctg accttttctt tatatttttg tgactgactt
      241 atttgatgag acaaactcgc tcctcacctg acaagtggat tgctgtgtga ggaggagatg
      301 cggtgaaaaa agatcgattg gtggaggtcg ctcggattca cttctggttg cacaatacaa
      361 ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg tcgatattcc
      421 aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg atttattaaa
      481 tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta tcacatttca
      541 agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg atgcagaaaa
      601 atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg atggtaatac
      661 tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga ggatcagaag
      721 gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt caatattcaa
      781 gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc cagaaagtgt
      841 gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac aggtataaat
      901 agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa gtgacaagca
      961 cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag cagctgcggg
     1021 tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag ctggagaaaa
     1081 gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag ctgcagaaat
     1141 gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct tagataaata
     1201 ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc taaaagtaga
     1261 taaaaaagac gattctactt acagtttttc atataaagca aagatgaata cctcattagg
     1321 tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc aaaccacgat
     1381 caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag taagcctgac
     1441 cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat tagcaacaac
     1501 aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag gcgaaaaaac
     1561 agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta tcaatcaggc
     1621 gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca ttgatggagc
     1681 aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat attatccttt
     1741 gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag aagatattga
     1801 taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg agatggcttt
     1861 tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg cagacggtgt
     1921 agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca aattgacaat
     1981 cgatgcgaag gcacaaaaaa tagcttttga cagtctagga ggaaaagctg catcaactgt
     2041 tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa gctatgatcc
     2101 aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag aaaatcctga
     2161 acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt ttaaaatgat
     2221 tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt tgacgatcaa
     2281 cgggcttaaa tggcaaaaag acagttcttg gggatctttt aaaacaacgc gtgtcagtag
     2341 tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata tatatgcggc
     2401 acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata aattcatttt
     2461 tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta atgaagatag
     2521 ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac ttctaattac
     2581 tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac ttgtctatcc
     2641 taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg aaacagcagt
     2701 acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta cagcacattc
     2761 tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa tcaaagaaaa
     2821 acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg ataaccaagg
     2881 ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa ccaaacgagc
     2941 atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt tttaagtaca
     3001 gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt tttcacgatg
     3061 tctctttcac aacttctttt tgatttactc acacttcata aacttttttt gtaaaatatt
     3121 tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa ggtgaatagt
     3181 actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa gccatttctc
     3241 cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa ccggcaatct
     3301 gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa
     3361 taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa
     3421 atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact tcttctttaa
     3481 cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag ctaagcccaa
     3541 caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg ataatagaag
     3601 aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata
     3661 agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa gaagcattgc
     3721 tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg actaaaatgt
     3781 catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact acgtttagtt
     3841 cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc ccaaagaaaa
     3901 tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga
     3961 agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc aaaagagata
     4021 aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc gaaaagtcat
     4081 ggactagaat atcaggtttg acccaattca atccagcttg gcctagcaaa acaccaacaa
     4141 gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag ccaatcgttg
     4201 ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt cctttgtata
     4261 aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa
//
LOCUS       DAZKNM010000179         4294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000179 DAZKNM010000000
VERSION     DAZKNM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.185"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..791
                     /locus_tag="ACG8M9_002475"
     CDS             <1..791
                     /locus_tag="ACG8M9_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0379183.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            888..3137
                     /locus_tag="ACG8M9_002476"
     CDS             888..3137
                     /locus_tag="ACG8M9_002476"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0379184.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3140..>4294
                     /locus_tag="ACG8M9_002477"
     CDS             3140..>4294
                     /locus_tag="ACG8M9_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0379185.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1393 t
ORIGIN      
        1 tttgtgggta ttcaaaaaca gaacctagac atagttttgg accagctatt cgagaacaat
       61 acttaataca catagtgcta gaaggtgaag gctattattc aattaatgat caaaaatatt
      121 ttctgaagaa aggccaagga tttgttatcc caccaggagt atctactttt tatcaagcag
      181 atgaaaaaaa tccgtggtca tatgtatggt tgggtttaag tggcaatctt gtagaatact
      241 atctaaaaga aattggtgtt ggaaagaatc atcttacctt taaagtcgaa aatttaaatt
      301 catttaaagg attaattttt gaaactttat cgtatgatca ggacaatcta gtcagtgaat
      361 tgtccagaca aaaacaagtt tataaatttt tagaactcct cttaaattca gctagttatc
      421 tgaacgaagg gataattact aaaaagatga atccttttgt tcgtcaaaca attgaaataa
      481 tacgtgagaa tgcgtatcaa aaattatctg ttaaagatat tgctgtcagt ctatcgatac
      541 acgaaaatta tttatcacga ttgtttaaga atgatatggg aatatcaata aaagagtata
      601 ttagtgaaat caaaatttct attgcaaacg atctgttatc atcaactgaa tattcaatcc
      661 agcaaatcag tgacaagata ggattctcaa cagtacaatc tttttctaaa gcctttaaga
      721 aagcaaatgg tgtttcaccg ggagtatatc gaaaacgtag attaggtcta ggaatcatta
      781 atggtgaata aactaaacat ttgattttga taaatattcg ttactttaaa cgcttactat
      841 ttatgtgagc gttttttata atgcaataaa gaaacggagg agagcttatg tataaggata
      901 atgaaatgaa tattatttat cgatcagaaa accaacaatt tcatttgttt aatcaggaga
      961 taagttacat ttttaagata tcggaagatg gtaaattatt gcatttatat tatggtgaga
     1021 acttaccaga taatgactat agtcatctaa ttgagatgca tcatcggcct atgacaacct
     1081 atagaaatga aaacgatttg ctatattcgt tagagcactt aaaacaagaa tttcctgaat
     1141 atggtacaac agattttaga catcctgcta tttcattact acagaaaaat ggctctcgct
     1201 tatcagattt tgtctatcaa gggtacgaag tggaagaagg aaaacctaaa ctagaaggtt
     1261 tacctgctac ctatgttgat aataatgatg aaagcaaaac attgaaagtt tatattttag
     1321 attcagttac aaatgttcaa gtagaattat tatatacaat ttatagagat tatcctgtta
     1381 ttactcgtag cgcgcgagtt atgaataaag gaaaagatat tcaaataatc gagaatatta
     1441 gcagtttatc attggattta cccgatgcaa attatgaatg gcaacaatta tctggtgctt
     1501 ggggtagaga aagaactatt aagtcgcgta ctttacaaca aggaattcaa tcaatcgaat
     1561 ctactcgagg tatttcaagc catcagcata acccatttgt tactttaaaa agaaaaaata
     1621 ctgatgaaaa tcaaggagaa gctattggtg cagcactagt ttactcagga aacttcttaa
     1681 ttcaagcaga agtagataca tgggatgtta ctagactaca agttggcatc aatccatttg
     1741 gatttaattg gaaactcaat ccaggagaaa tatttacagc accagaagcg attctggttt
     1801 attcaagtaa gggtttaaat gccatgagtc aaacttttca tcaactattt agaaaacgct
     1861 tagctcgtgg taagtggcga gaaaaagacc gtcctgtctt aattaataat tgggaagcaa
     1921 catattttga ttttgacgaa gaaaagttgg taaatattgc taaaaaagca tttgatgttg
     1981 gtattgaatt atttgtatta gatgatggct ggtttggaga aagagaaaac gattgtgcag
     2041 gattaggaga ttggcatgta aatcctaaaa gactacctag aggaatcggc agcctttcag
     2101 aaaaaattcg tagttttggt atgaaatttg gtttatggtt tgaacctgaa atggtgaata
     2161 aggatagtga gttatatcgc acacatccga attggattat ttctacacct aatcgaaaac
     2221 ctaatcatgg taggaaccaa tatgtattaa attttgggtt agatgaggtt gttgaaaata
     2281 ttttcgatca aatgtgtaaa atcattgatg aatcaaactt agattacatt aagtgggata
     2341 tgaatcgtcc attgactgat gtatttgaca gtcacttgtc agcagatcaa caaggagaag
     2401 tattccacag atatgtacta ggcgtatatc gcttatatga aaaacttata acaaaatatc
     2461 ctaacatact atttgagtct tgttcatcag gtggtgggcg ctttgatcct ggaatgcttc
     2521 actatgcacc tcaagcttgg gctagtgatg attcagatgc aattgaaagg ttaaaaattc
     2581 aatatggaac tagtatgctg tatcctcttt cgtctattgg tgctcatgta tcaattgttc
     2641 caaatcatca aacaaaccgt ataacaccta taaaaactag aggaaatgta gctttttttg
     2701 gtgcatttgg atatgagctt gacttaaatg aattaagtga aggcgatttg aggattgtaa
     2761 aagaacaaat tgccttttat aaaaaatata gaaccataat tcataatggt actttttata
     2821 gactattatc accattcgat gatgataatc aaaccgcgtg gatggttgtt agtcatgata
     2881 agaaaacggc tattgttgct cattataaaa cattaaatga ggtaaatgct ccttaccgcc
     2941 gactgaaatt aaaagggtta gataatgacg gactttataa tgtaggacaa aatagtttgc
     3001 gatctggaat ggaattgatg agagcaggac tagtctgttc agatgcatct agtggacaaa
     3061 taaatgatca gaatcgacgc ccagaaacat ttgattttga ttccaagatt tgggttataa
     3121 cacaggagga aaagtagaga tgaataacca aaaaagctta cttcaaaaag gagcaatctt
     3181 aagtatttca ctaatgttaa caagctctca agcaattaat ggtgttattc cacaaatgaa
     3241 acagtctttg gggatatcac aaagtcaaag tgaactttta ggaactgcac caagtataac
     3301 tgttattttg tttatcttat tatctagtta ctttgccaaa agaataggaa tgaaaaagac
     3361 aataatttta ggtttattat tagctggtat aggaggaatt attccagtat ttacagctac
     3421 cttccaaacc gttcttatta gtcgagtaat attaggtgcc ggtttaggag tatataattc
     3481 attggctgtg acatatatca gctctttata ctctggcaat actagagcaa cgctattagg
     3541 tattagaaat tcaatggaag ctttaggtca gacaatcctt atttttcttg ctggagtttt
     3601 agtgaacatt aattggacgg cttcgttttt agtctatgca attgcgtttc ctgtagctct
     3661 cttattcgcg ctaaaagttc ctgaaattag agatgaaaat agtgacatac ctgaaaatca
     3721 tgtaaaagaa aagatgaatc caatggtttt tgccctagtt ctatttgcaa tcttactcgt
     3781 aatgaatagt attgctattt ctgtacgatt tgcatcaatt gcaactgaaa ttaaaggggt
     3841 aaactttaat gctagtaact atttagctct aatgcctatc ttaggaatac tagcaggatt
     3901 tatgtttgga ccaataaata agtggatagg aaaaggaacc ctatacttgg gaattatatt
     3961 ctttataatt tcaaatttgt taattgctgt gtcaaacggt aatatgacgt tcctgctaac
     4021 gggtttattt ttatcaagta taacaggatc atggtgcttc ccatttatct ttagtaactt
     4081 agataaagtc actacaaaga atacaattaa ttttgctact tcattgattt ttattggctg
     4141 taatattggt aattttatag ctcctattgc tatgcaatta gcacaatttc taacaagaag
     4201 tacaaatttg actgctccat tttttgtatt cgcaggaata tttattgtga tcctatttgt
     4261 aacatttttt gcgacaaata aaaaaaatac taga
//
LOCUS       DAZKNM010000180         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000180 DAZKNM010000000
VERSION     DAZKNM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.186"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8M9_002478"
     CDS             complement(37..1257)
                     /locus_tag="ACG8M9_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0379186.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8M9_002479"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8M9_002479"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0379187.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8M9_002480"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8M9_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0379188.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8M9_002481"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8M9_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379189.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKNM010000181         4277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000181 DAZKNM010000000
VERSION     DAZKNM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.187"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..892
                     /locus_tag="ACG8M9_002482"
     CDS             2..892
                     /locus_tag="ACG8M9_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0379190.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(965..1789)
                     /gene="nadE"
                     /locus_tag="ACG8M9_002483"
     CDS             complement(965..1789)
                     /gene="nadE"
                     /locus_tag="ACG8M9_002483"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0379191.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1801..3273)
                     /locus_tag="ACG8M9_002484"
     CDS             complement(1801..3273)
                     /locus_tag="ACG8M9_002484"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0379192.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3910..4272)
                     /locus_tag="ACG8M9_002485"
     CDS             complement(3910..4272)
                     /locus_tag="ACG8M9_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0379193.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
BASE COUNT         1242 a          866 c          742 g         1427 t
ORIGIN      
        1 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
       61 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
      121 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
      181 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
      241 cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
      301 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
      361 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
      421 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
      481 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
      541 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
      601 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
      661 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
      721 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
      781 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
      841 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
      901 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
      961 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
     1021 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
     1081 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
     1141 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
     1201 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
     1261 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
     1321 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
     1381 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
     1441 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
     1501 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
     1561 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
     1621 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
     1681 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
     1741 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
     1801 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
     1861 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
     1921 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
     1981 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
     2041 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
     2101 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
     2161 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
     2221 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
     2281 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
     2341 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
     2401 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
     2461 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
     2521 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
     2581 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
     2641 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
     2701 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
     2761 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
     2821 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
     2881 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc
     2941 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
     3001 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
     3061 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
     3121 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
     3181 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
     3241 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
     3301 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
     3361 aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat
     3421 cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg
     3481 agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct
     3541 actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca
     3601 acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat
     3661 ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga
     3721 caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat
     3781 acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt
     3841 ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa agatgattat
     3901 tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt
     3961 tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt
     4021 gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt
     4081 gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag
     4141 cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa
     4201 gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc
     4261 gattagcttc atctcat
//
LOCUS       DAZKNM010000182         4246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000182 DAZKNM010000000
VERSION     DAZKNM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.188"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..483
                     /locus_tag="ACG8M9_002486"
     CDS             46..483
                     /locus_tag="ACG8M9_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379194.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            578..1462
                     /locus_tag="ACG8M9_002487"
     CDS             578..1462
                     /locus_tag="ACG8M9_002487"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0379195.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1684..>4246
                     /locus_tag="ACG8M9_002488"
     CDS             1684..>4246
                     /locus_tag="ACG8M9_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0379196.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1391 a          712 c          959 g         1184 t
ORIGIN      
        1 taaataaaga aaacgatatt aaaaaatgca cgtagaagta gataaatgga gtggttttgc
       61 aaatttcttt tatttctaga agttattcac gtatacggat ttgtttttct cacaaataaa
      121 aaggcaggaa gtacaatgga tcatttgtat ataaaatata ttcatcaatt ttccgttttg
      181 gtattaggca cactaattat agcggggatt gttgcattta ttattcaagg tcgtgacata
      241 ttaacagtac atcaagagtt tttcagttat gcatttggta ttcctctgat cgctataagg
      301 tctgataaca aaaagtggaa gaaacgctat tacattctct tgctattttt gtgtttttat
      361 ctgccaccta agatttttac tttattaggt atacaatgcc tatcattttc ttttatttcc
      421 tatatcattg ttttaatttt cctcagtttc atagtatggg tattttatct tgtcaaaaaa
      481 taaatgaatg tttcatgaac agtacttcat cacagcctaa tttttttctt tatgctatcg
      541 ttatattaaa gaaatcaatc agaagggaaa gaaagtgatg aaaaaagttt tattagtggt
      601 aaaccccagt tcaggcggtg aacaagcaaa ggaatttgag caattagcga tagcaaaatt
      661 ggaatcggtg tttgatgaag tggtcgtctt gcatacaaaa aaagcagggg atgcaaaaaa
      721 ctttactcgc gaagcggcta cggaagggta tcatagtgtg tttgtcatgg gcggagatgg
      781 aacagtcaac gaaggaatca gtggaatcgc tgaacaagaa catcggccaa atttcggatt
      841 tttcccattg ggtacggtga atgacttggc tcgagcatta gggataccgc tagaacctga
      901 ggaagcgatc aatcattttt cgattgaatc agtcaagcca ttggatattg gaaaaataaa
      961 tgacgactat tttatgaatg tcgtcgcaat tggctctatc cctgaagcaa tcaatgatgt
     1021 agatgcggag aagaagacga aatttggaaa actagcttat ttcatgtcag gaatcaaaca
     1081 gttagcttcc acacaatcct actcgtttca cgtagaagtt gatggaaaga aggaagaaat
     1141 tgagagcagt atcttgctga taggactaac taactcagta ggcggttttg aaacattact
     1201 tccaaatgca aaagtggatg atggtaagct ccatttggtt tatttaaaag attcttcttt
     1261 gatcgatacg cttaaaacat tgccagacct attaaaagga gtagatgaat ctacgaaaaa
     1321 tctagcctat cttacttgtg agcaattagt tgtgtcatta gctgatgagg aagaattgac
     1381 gacgaatgtt gatggagatg aaggggataa attacctgtg acaatcaaag tgttaccctc
     1441 acatttacag gtttattgtt aaaatggagg ttgtgaccga agagttgtca ggaggacatc
     1501 ggttgttcgg aataagggac tgtgacaaga cttttgtcac agcctttttt tgtacaaaaa
     1561 agaaattttc acctaacaga aaaatttgcc ataaaaatac atattttaaa tatgaaagta
     1621 caattacaaa taaattaaaa cgttaaaata aaattgtcaa aacgtcagag aggatggtgg
     1681 agaatgaatc aatatgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg
     1741 atggtgacat ccgtttttgc cgatgtagag aaaacaagcg aatcaacgca atcagagaat
     1801 caaagttttg acaagactac tttttcagaa gaatcaatta cttctgaaac tacagaatcc
     1861 caaagtgagg cgcctacttc taatacaagg caagaagaaa ataagacaag tgagacctca
     1921 gaagtagctg aacaaacgtc tgatagcagt gaagaggtac aggttagagt tgtttcagcg
     1981 gatgggtaca gcgaagctgc cacaacagaa gaactgacgc agcttctcgc agacgaatca
     2041 gtagctaaga ttcgtttaac tcagcctttg actttagaca gggaattgga gatcaagcga
     2101 gatatcgtta tcgattttgg cggacttgct cataatttcg gcacacatca catctatatc
     2161 aatgagacac ctcagtcgat cgagtttcag aattttaaag gaacggcagc tcatccaggt
     2221 gggttgatac caaatatcga tggaaatgcc attatcattg cttatatgtc cgatttttgg
     2281 ggaaacagat atcattttac gggagaaata aaatttaccg ggacacttga cttatatgat
     2341 ggttctaaat taggcctgat ttacgcccct cgtgcaactg tcacattaga tggtgtttcc
     2401 ggtgtattgg atgtccaacc agtgaaagaa gggatgaatg ccgctccggg taaagcttat
     2461 ttttgtagaa gctatcagct aaatgtaatc aatggtagtc agctttatgg accatatcta
     2521 ggaaaattct atggttttct agattcaggt gatgagacgg gcagtggaaa aacagcacca
     2581 ggactccata tcttgagtgg ttcaaaggtc tctcttgact atgatcgggc agatggcgtc
     2641 agcgatgaag gagaggctgt cgatacgcta cccgggaatg tcacatttaa agtcagtggc
     2701 tcaggaagtg aattttcggt aaatacaaag atcaatatta ccaacgacaa caatcgcgga
     2761 attatccaaa tgagaggctc aggatcaaga gtcgctgtgt cgaatgatgg gaaaatcaca
     2821 atcaatacag cgactactgg cggatttcgc ctgcaaggag attctagtca aatccatgta
     2881 tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg gcgaccaacc tggaaacaat
     2941 gggatgcgtt ttgtagggaa aggactttct ttgatcgtag aaggagccgg atcagtcatc
     3001 gatattgata agcaaagtgg tcgttctagc gcagttcgtt ttgagaacgg tacgcaaaaa
     3061 ctaacagtaa ctaatggagg ctcattgaag gtaagaaatg caggggatgg caaatcttat
     3121 gtgaacagac tcaatcaagg aaaccaagcg atccaatttt atgacgcttc tggttttcta
     3181 gaaagtccag ggtcagctga ttttacggtt actggagaaa aatcaaatat cgatatacgc
     3241 gcagattccg gagctaccgt agatacaact ggagatattg atttgaactt tatggctggc
     3301 gaaaaaactt atttagtcat ggtcgggaaa actggggaag ataagactgg catcatcagt
     3361 ggggatgtgt tgaatgtaga cttagaatct cctacctatt tcgatttcca aaacaaacgg
     3421 atcggaagcg agactaatgg cataggtggc tgggtatttc aaggaaatgg cgattcttct
     3481 ttgaagatcg ctcattcaga aattgcttta tggcgaaagg caggagtaac gttcgataac
     3541 tttgaaaaag atccggatta tttttctgat atgacagact tatcgatcaa tggaaccgac
     3601 ttaggtaatt ttgtatcaag ccctagtgca ggtttaacaa atgaatttgc tcagacaggt
     3661 gccggaatga aaaattataa tcgaatcagt gccaataacc agcttccgat gattgaatcc
     3721 ttaagagtac caacgaatgc agataaaaaa atctatgggc atgtcgttgt tcccgaaggt
     3781 gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag aagtgacttt acgtattact
     3841 tatgcagata cgacgaaatc acctgtagaa attactgctc tcacacaagg acaaacagat
     3901 gaaaatgaag ggacaggata taatccgtgg ggagaaggcg aacaaggcgg tttgtttgag
     3961 gttcaagtac ccaacgaaga aagactaaca acgggggatc aagtgcaaat agttgctgct
     4021 aaaaaagtaa gaggcggcca agaagtgaca gaactggact ctgccaaaac gacagtcgat
     4081 gtgattcctc ctgagcctgc taaattaagt accacattga tcaatgcagc ttctcgctca
     4141 atcacaggta caggcgaagc aggagctttg gtttcattga aaaaaaatca agctgttttg
     4201 tccgaaacaa ctattgatga tacaggtaaa tttgttcttt ctatcc
//
LOCUS       DAZKNM010000183         4217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000183 DAZKNM010000000
VERSION     DAZKNM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.189"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..1886)
                     /locus_tag="ACG8M9_002489"
     CDS             complement(120..1886)
                     /locus_tag="ACG8M9_002489"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379197.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1846..3600)
                     /locus_tag="ACG8M9_002490"
     CDS             complement(1846..3600)
                     /locus_tag="ACG8M9_002490"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379198.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3710..>4217)
                     /locus_tag="ACG8M9_002491"
     CDS             complement(3710..>4217)
                     /locus_tag="ACG8M9_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0379199.1"
                     /translation="FSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRKGIFTI
                     YTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAA
                     GQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGR
                     KVLKKYKK"
BASE COUNT         1420 a          801 c          592 g         1404 t
ORIGIN      
        1 tcaatcatac tgattctttc gtgtattcaa aattagatgc tgtttaataa aattattctt
       61 tttgaaaaag aattataact atatcccaaa aattgtaggt taatttaaag cctatttcat
      121 catcgtgatt gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg
      181 agatttttgt atatttctga ccttcgatac aactcattat gagtaccttg atcaactagt
      241 tttccttgat ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat
      301 ataattagta ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca
      361 ttttccgcat ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa
      421 aaagcccgtg ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct
      481 ccgaccaatg tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa
      541 cgagctgctt ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc
      601 aaaatacttg tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa
      661 gaagctggag atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa
      721 tcatacagtc tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact
      781 aacgcagtca cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg
      841 ttatcatatg agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct
      901 gatccctctt ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa
      961 taaaatattt caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg
     1021 gctagtaagt aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca
     1081 cctactagaa tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact
     1141 gaaaaagtac ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa
     1201 agtctgtctt gaactttttt agatagatta aagctattaa tctcttgctg caattctata
     1261 gtttcttgaa attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc
     1321 gtttgggatt tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca
     1381 gctaatccca ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga
     1441 gccataccgc cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca
     1501 gacatgatag tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa
     1561 tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac tttctttata agtactatta
     1621 tataagtttt catactccct atctaacaac caatacatga aaagcaaagt aacaaacgat
     1681 acggcaaaat aaagccaacg gggctttgta ttttctaaaa ctaattcgtc tatcagcatc
     1741 attataatga atgctggtac catattaatg cagtatacaa aaaaactaga aatagaagct
     1801 ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt tgtttttata aaaacttttc
     1861 attgtcaagc ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg
     1921 atgtttagcc attaattcat tatgatttcc acgttctact attttccctt ctgacaagac
     1981 aagaatttca tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt
     2041 ttctttcatc agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga
     2101 tgcactggct tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc
     2161 tatacgttgt ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc
     2221 tctttcggga aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac
     2281 tttacttgct ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa
     2341 tagtttcgta tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg
     2401 atttaaaggc ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat
     2461 tagtttagct atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt
     2521 ttgttctaaa actaagttga aattttctaa aacacactct tctttgtatg aaaagctgac
     2581 attttcaaat gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt ttagttggtc
     2641 ttgcttcatt tctaaataaa gggagtctag tgtttcgact gcatatcttg cttcaaacaa
     2701 gtgcatactt acatacatta ttctcatgaa agaaacaaaa ataattccac ttaaaaaaaa
     2761 caacatcaac aagtcaacag ctaatatctt gggagtggta agagaagtta taaaaaaggt
     2821 tataggaact atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa gaacaaaagg
     2881 tgttttccca ctcaaagaat acttatatgc aaacttagca taatcgttaa ttgctttatt
     2941 caaggcttta aatgatgtta ctttagcacc aaatatcttt ataacttgaa ttccgcggat
     3001 atattctacc gtttcagagc ttaacacatc taacgattct tgatattttt gaataaaatt
     3061 tgtattctct gtcattttct ttaacaagaa tacaccaatt actaacatgg cgattagaac
     3121 caatccaatt ttaaagttaa tgaaaaagcc taaggcaact gataaaacag gtgttactat
     3181 ggcttgagca ctatctggaa tcaagtgagc aattgccata tgtgttttag ccgcgttgtc
     3241 atcaattgtc tttcgaatat atcccgaaga atttaaatca aaaaaacgaa aacttgcttc
     3301 tgttaaaccg ctaattccct tttttctcaa attagtctct aagcgaaacc caaggatatg
     3361 agaaaataag ccagaaaaaa tataaaataa agctcctaaa gttaaccaag caacagtttg
     3421 aaccgcataa ctcttagcta aatattcatc tcctacaata attagagact gaagaaattt
     3481 gtaaatactg taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc
     3541 taaccatccg tagtatttca ttttagggac ataactaaat aattttttgt atactttcat
     3601 tttttcctcc cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg
     3661 taatagcgta aattagagat gtacaaacag ataaaaggta aactacgttc tattttttgt
     3721 atttttttaa gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta
     3781 acactgaaat gattccccat atacctaccg catagtggat cattgcatcc attgttgcag
     3841 cagattgtcc tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa
     3901 tccactgtcc ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg
     3961 ctcgatcata tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac
     4021 tgataaacca aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg
     4081 tgtaaatagt aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc
     4141 cagataataa tccaaaaatc ccgggggata tgctgtgacc aaacgcccca ccaatactac
     4201 taaccattat tgaaaaa
//
LOCUS       DAZKNM010000184         4186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000184 DAZKNM010000000
VERSION     DAZKNM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.190"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1034)
                     /locus_tag="ACG8M9_002492"
     CDS             complement(<1..1034)
                     /locus_tag="ACG8M9_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFQ0379200.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETT"
     gene            complement(1038..1448)
                     /locus_tag="ACG8M9_002493"
     CDS             complement(1038..1448)
                     /locus_tag="ACG8M9_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0379201.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1466..1957)
                     /locus_tag="ACG8M9_002494"
     CDS             complement(1466..1957)
                     /locus_tag="ACG8M9_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0379202.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1967..2758)
                     /locus_tag="ACG8M9_002495"
     CDS             complement(1967..2758)
                     /locus_tag="ACG8M9_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0379203.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2751..3524)
                     /locus_tag="ACG8M9_002496"
     CDS             complement(2751..3524)
                     /locus_tag="ACG8M9_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0379204.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3680..>4186)
                     /locus_tag="ACG8M9_002497"
     CDS             complement(3680..>4186)
                     /locus_tag="ACG8M9_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0379205.1"
                     /translation="FKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDS
                     CEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFL
                     HLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIY
                     TIFEQTIF"
BASE COUNT         1316 a          853 c          562 g         1455 t
ORIGIN      
        1 gttgtttcaa atagaaaata atcttcgatt ttaggaacaa ttactctttc tttttttcca
       61 aattctttct ctaacttaaa acttgaaaat ggagtgattt ctttaaaatt ctcaattatt
      121 ttttcaggca gtttaacacc aagagttttg tctgtctttt gaataatttg atgtggaaca
      181 agtgcaccgc cccaatccca gttattttga tcgttctccc cttcttttgt tgccacccag
      241 ccagttagaa aacggccagt ttcattagtt ccgactgttc ttgctgcata ataggctttt
      301 ccgtcgaaag cttcgtctac tggtgatttc caatctgaaa ataatcctgc tccgactcga
      361 tatttagtct ttttatcttc acaatactct gaaaaaagta aataccacgt tccatcaatt
      421 tcaaatatat caggcatttc aatcatgtta aattcattat tagcccagta gtctcctttg
      481 aaattccaat cttttaaatc ttcggattca aaatagacca atctccccgt cggctttttc
      541 ttacttgctg gttttctagc tcctagtatc aaaacgtatt ttccatgatc ttgatcaaaa
      601 attgcaaaag gatcgcgcca atcattctta tcatatcctt cttgaggaac aagttttgct
      661 gcttcgccat cctttttcca atgaattaaa tctttagaac ttgcaatcat taacacttct
      721 gaagtttttc catccaactt agctttgcga ttatgtccag tatacaaagc taaatactca
      781 ttgattcctt ggacaatact tccagcgtaa atgtattgat caatgtcatc gttgccacca
      841 cgatgaatta cttccccgta ttcttcaaat ttcacaaaat cagtggttgt aactagtgac
      901 cacccaaaag gatcagttaa aggtttagga tttctcgtat ctcgttgatg aaatagataa
      961 aaagttccat ctttataaaa aggcatgcaa tccccaaacc aaaaatcttt aggcttgtaa
     1021 aaaatttttt gcatttatca ctcatcctct tcgttagtat gtctttgcat atctgcaaga
     1081 cacgcaattg tattcatatt tcttagattt tcgatgatat catcgctttt ttttccttgt
     1141 aatctcaaat tgacagcctc aatcaaaatt cctaaattca tgccaccaag aagttgaaac
     1201 ccattttgaa atacttcact tcttttagca aattcgttga aaggtgttcc accaatcaag
     1261 tcagcaaata ccataacttc ttgatttttt acttgagaaa ctattttttc aatgttttca
     1321 cggaattctt caatgcctgc atctccaagt gttaccacat gacaatttaa attatcgcca
     1381 aaaaacattt ttaatgtatc tacaaatcca atggcaatat ttccatgaga tgtaattata
     1441 aactccatcc actcatcctc tgttcttaat aaataaaatc ctttaaaaat tcttttgaat
     1501 ctctaggagt ttgtatcact gtcacttcaa ctccatcact aattagttct ttaaaagcct
     1561 ctctctcttc ttcagtaata tttatcgtcg gacggatgcg ttctgttcca ttccttcttg
     1621 ataaatttcc tacgtttaca cttgttattg gtaaaccgtt atcaatcaaa aatttaacat
     1681 cgattggaga aactgcaaca ataagaactt tttgtccttc atatttacca gaaagaatat
     1741 ttttaactgc tacttccctt gtaattattg aagttctaac attaggtggt gcagcgaggc
     1801 gaactacagc tttttttacg tcatcattgg ctacttcatt attaataacc ataattctgt
     1861 tagcactcaa ttgatttgtc cattgtgttg ctacttgtcc atgaatcatt cgatcatcaa
     1921 tacgaacatg tataatacct ttcatttttt catccatatt caacacctac ttcaaaatat
     1981 ttaacgctct taacgcaata gaaactacta agattaatag aattacataa actggtttaa
     2041 cctttcgtcc taacaaataa tacacaattg ctactacaca tgcaggtaat aaaccaggca
     2101 taacttgatc aaagatttca tttccggtca ttgttaagtc gccttgttta aattcaaagc
     2161 caacattcac ttttacaact gtggcaatga gtgcaccgac caccattaac cctaaaattg
     2221 atactgcatc agtaatagac tttaattttg tttcaatttg agttagtagg ctcgcacccg
     2281 attctttccc agcaaggtat aaaggataag cagtaatcct taataaaatt gatgccagta
     2341 tccacaaaat aattccaaca aaactacctt ccaatgccat attagctgca attgctccaa
     2401 aaatagtcca tggaataact acaaatacac tatctccaat tcccgctaaa ggacccatta
     2461 gacctgtttt cagagcactt actgcttcca aagacttatt tttttcttct ttttcaattc
     2521 caacattcat accaataatg taaggcgctg tatacggcgt agtgttaaaa aattgtaggt
     2581 gagctaaaac ggattgtttc attccttcag gatcatcttt atatgtgtcc ttgataaaag
     2641 gaagcataga atacgcataa cccaaccctt ccattttttc ataatttaat gaagttgcac
     2701 cattgagaat ccaccggaaa attacttgtt tgtcgatttt tgtcagtttc ttattcatct
     2761 tcgtcaaaac ctcctgttgc agctggtgct acttgagtat tatttgatct atttttatat
     2821 acgattagtg cgattgcagc gccaagtaac gaaatcccta aaattggtat ttggaaataa
     2881 actgccaaaa cgaaaccaac taacaaatag gcaaagtaat cttttgtagg taaatattta
     2941 agtagcattc ctatcccaac agcaggtaat agtccccctg ctacctttaa accaccggta
     3001 acccattcag gaatataatt gattgcactt cccactaacg ttgggcctaa tatcacagtt
     3061 aacaaaacag gaattccaga tacaaagctt gtattaattg atgccaaata ttgcatccac
     3121 tctaattttt gataatcttc tttttgtgcg aatttttctg ccttttgttg aaaaaaaatg
     3181 tttgttgacc actttagaac atctagttgt acaattaaca ttgctaccgg tattgctaaa
     3241 gttattccta tagaagcatc ctggttagtt gagattgcta aataagtacc gatcaatgct
     3301 gctgtttgat aatccggtac tgatgcacca ccaaaactag taatacctag tgtcattaat
     3361 tgcaaagtcc caccgatata aagtccagtt tgaatatctc ccataataag tccggtcaag
     3421 aaacctgcaa cgacaggttg ggaggttccg attttgggcc cttccttatc ccagttaatg
     3481 aaaaatccat acaacacaac taaaaatgtt tgccaaaacg ccattttgtt ctctcctttc
     3541 ttcttatact tttatataag caattactgt gccaactaac attatccaat aaagcgggaa
     3601 aatacagtaa atattaatta aatgagcgtc taaatatact ttggacaccc tttcattttt
     3661 ttagacgcta gtatgctatt tagaaaatag tctgctcaaa aattgtatag attaactcat
     3721 actctccttt gggtatttca atacagtaaa tatctttgat ttctgaaaaa aaatcttttg
     3781 atacatttaa aaaagtatca aatagttgcg ctccctcttt caaatgttca tttgttattc
     3841 catcaccaat cataattcgt tcaatcattc cagaaagatg tagaaataga ttaattctca
     3901 gaaaacttgg gattttgaca tcgtaatatt tttcatacaa atatagtact ttttctatct
     3961 cattgaatac ttttttggaa tctaaaaagc gaagcttagt actagcgcct tcaatagtaa
     4021 agagtttaac taattcattt aagcagaact cacagctatc cgagtccata tacttattta
     4081 atttttctaa ggtttgattc ccattaatta gattttctaa atttaaacaa tcgattcctt
     4141 gaatgtaaaa ttcagtggtt gaaagaacac aaattgtatt cttaaa
//
LOCUS       DAZKNM010000185         4126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000185 DAZKNM010000000
VERSION     DAZKNM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.191"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..125
                     /locus_tag="ACG8M9_002498"
     CDS             15..125
                     /locus_tag="ACG8M9_002498"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379206.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
     gene            130..2259
                     /locus_tag="ACG8M9_002499"
     CDS             130..2259
                     /locus_tag="ACG8M9_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0379207.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            2256..3263
                     /locus_tag="ACG8M9_002500"
     CDS             2256..3263
                     /locus_tag="ACG8M9_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0379208.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            3280..>4126
                     /locus_tag="ACG8M9_002501"
     CDS             3280..>4126
                     /locus_tag="ACG8M9_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0379209.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTT"
BASE COUNT         1357 a          668 c          942 g         1159 t
ORIGIN      
        1 aaaacggtca atgcttgatg caagacttgt atgggcgagt aggtgtggtt caagttcatc
       61 cagtctttga agaactcttc catgcttttg acactcgtcc gccggttcaa gagagaatgg
      121 agtgagggga tgaaaaagaa actttttcgg attgtgggaa tcgtttttat tagcatcgtc
      181 ctattgacag ttctattgag tttagtcggt acggtagctg aggcgacagg tctggttgat
      241 gatacagttg aagccggaaa cctttattcc aaatattctc tgaacaatta tcaactggat
      301 ttctttgtgg atagttcttg ggattggtta ccttggaatt ggggggatgg cttagggaaa
      361 agcgtgatgt atggtcttta tgccattacc aattttattt ggacagtaag tctgtattta
      421 tcaaacgcca cgggctatgt ggttcaagaa gcttacaagt tggatttcat ttcggatacg
      481 gctaaaagta ttggaaagaa tatccaaact ttagccggta tcactgaaaa tggattacag
      541 acttcgggat tctactttgg gttcctatta ttgatgattt tagctctagg agtttatgtg
      601 gcgtataccg gattattaaa acgagaaact accaaagcag tccgaaccgt cattaatttt
      661 gtgatgattt ttctactctc gggttctttc attgcgtatg cgcccaccta tattacaaaa
      721 atcaatgact ttagctcaga cgttagtgaa gcggctctta gccttggaac caagattgtt
      781 gtacctaatt cagaaagcca gggaaaagat agtgtggatt tgattcgaga cagtttattt
      841 tcgattcaag ttcaacaacc ctggttacta ttacaattcg atgattccaa tatagaagaa
      901 attggcgagg gtcgggttaa taagatttta tccgtgagtc cggatgaaaa taagggtaag
      961 gatcgggaag aagcagtcaa agcagagatt gaagataatg acaatgcgaa tctcagtatt
     1021 accaagacca tgaaccgttt aggaaccgtg gtctttttag tgttgtttaa tattgggatt
     1081 tccttttttg tgtttcttct gacagggatt atgttgttct cacaaatcct tttcattatc
     1141 tttgcgatgt ttctgcccat tagtttctta ttgtcgatgt tgccgacgta tgagagtttg
     1201 ggcaaaaggg cgattattcg tttgttcaat acgattatga tgcgagcggg agttacctta
     1261 gtcattacga ctgcattcag tatctccacg atgtttttca atatttcggc cacctatccc
     1321 ttctttatgg tagcttttct acaaattgtg acctttgccg gtatctattt caaattaggt
     1381 gacattatga gcatgtttaa ccttcaaagt aatgatagtc agtcaatggg aaggcgagtc
     1441 atgcggaaac cgcaaatgtt gatgaatcga aaattgcgac agcttaatcg aaatgtgggt
     1501 cgaacttttg cttttggtgg cggtgcagca gttggtggga aactagctac cagttctaca
     1561 aaagggaaag gtagttcaaa aatcaataga gtgtccaata gaaagaacca ttccgcgaga
     1621 agcattaacg agctaaattc tgatctgaaa aaacaagaac aaccagtggg cacgaagaaa
     1681 gaatcacgaa taaatttagt tggtagaaaa gttggaaaag tactcgatac aaaagaaatc
     1741 gtgaaggaca aagcgaaaca agcaaaaaat caagtaaccg atgcaccaac taacttgaaa
     1801 tacagccttc ataaaggcat ggaaaagacg aaaaaagtac cagaggactt taagcgaggg
     1861 cctttcgaag aaaaagcaaa tcgaggggag ttgcgagaaa aacaacgaca acgaagagac
     1921 gcaaaaatgg atgaaaaacg caaagtattg aacgaagcat ggaatcgtca tgagaaagga
     1981 agggaaaatg ttccgataaa aaaggagata aaaccacaga aaaatgaact accagaaaat
     2041 aggtctttaa aaccaagggt acaagtacat tcgaatcctg aaatcaaacg gaaactttct
     2101 agtcaagaag tcattccaaa cggaagcact cagccactaa gcaagaacaa ttcctttcaa
     2161 caagtgaaac agagattcac ttttccaaag cgaaccaatc aacaagtaca aagaaaaaag
     2221 aacactcgcc caaaaccaaa gccaggtgag aaaagatgaa cttaaaaaaa ttgagtatta
     2281 tttcgttaac cttcctgacc atctcttttg tgggcatctt gatgtgtgtg ggtttgttgt
     2341 ttggtgagga cggtgaggga agtggtagtt ctggaacttc tcctggaggt agtagtgtct
     2401 cagaagtagt tttgaaacac cgttcaatgg tggaaaagta tgccaaagaa tcagatataa
     2461 cagagtatgt ttcgattctt ttggcgatta ttcaagtcga atctggaggt acaatggaag
     2521 atgtcatgca aagttctgaa tcagcaggtt tatcaccgaa ttcattaagt accgaggcat
     2581 ccatcaaaca agtgtgtctc tatttttcgt ctttggttaa gtgttccaaa gaactaggat
     2641 gcgatcaaga tagtatcatt caggcttata actatggtga cgggtatttg gattatgtcg
     2701 cgaaaaacgg aaagaagcat agttttgctt tagccgaatc tttttcaaaa gaaaaatcgg
     2761 gtggtcaaaa agtcgattat cccaatccca tcgcgattaa ggaaaatagt gggtggcggt
     2821 ataactatgg caatatgttt tattgcttat tagtgaagca gtacctaact acaacacagt
     2881 ttgatgataa aacagtccaa ggaatttttg acgaggcgtt caagtatgaa ggaacggctt
     2941 atgtatttgg tgggtcaagt cccggaactg ggtttgattg tagtggttta actcaatggt
     3001 gttatgccaa agttggtttg aaactccctc gggtggctca ggatcaatat gatgctatga
     3061 ctcatattga cttaaaagat gccaaaccag gcgatttgat tttctttcat tcaacttatg
     3121 atgccggtac ttatgtgact catgtgggca tttatgcagg agagaacaga atgtttcatg
     3181 caggagatcc ggtgggttgg accaatctta ccgatagcta ttggcaacag cacattattg
     3241 gtgctggtcg gtataaacaa tagaaaagag gaatggaaaa tgaagataaa aattgagcga
     3301 agtaacaagg aaaagattcc aaaaaagaag aaagaacgag tagtatccgt gggcaagcat
     3361 cgaaaaatgg tgcttgccct ttggctttta ttgtcttgta gccttgcttt tggaatctac
     3421 aaaaacttca cggccattga tcagcataca actcatgaga aagtggtcgt gaaagagaaa
     3481 atagtgaata cctctggagt agaaaatttt actgtagatt ttgtgaaaga atatttttca
     3541 tggaagaata acaaggaggt aattgaaaag agaatgacta atcttgagca gtaccttaca
     3601 gaagaagggc tcgctttgag ccaagatatg gttcgagcgg atattcccac tagttccgag
     3661 gttgtatcag taaaaattct agatgttgaa aaacactcgg aagaatttgt cgtttcattt
     3721 gtggtagatc aaaagattac agaagggaaa aagacacaat caatttcttc tgcatatcgt
     3781 ctgatgatct ttgaagatga aaaaggcaat catattgtaa cgagtttacc cactatgatt
     3841 ggtaagccag aaaaggcaaa acataaagta agacaagtaa aaagtgattc aaaaattgat
     3901 tcaaagacaa cggaagaaat caccgagttt ttagagacgt ttttcaggct ttatccagca
     3961 gcctcagaaa aagaactaga gcattatgtt gaaaaagacg cgatgcaacc aattaatact
     4021 aatttgaaat ttgtggaatt agtgaatcct atttacaaac aaaagggtaa agcgattcaa
     4081 gtgattgttg gtgttgagta tcttgatgag ttgtccaaaa caacaa
//
LOCUS       DAZKNM010000186         4115 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000186 DAZKNM010000000
VERSION     DAZKNM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.192"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..373
                     /locus_tag="ACG8M9_002502"
                     /pseudo
     CDS             24..373
                     /locus_tag="ACG8M9_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(645..935)
                     /locus_tag="ACG8M9_002503"
     CDS             complement(645..935)
                     /locus_tag="ACG8M9_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379210.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1337..1573
                     /locus_tag="ACG8M9_002504"
     CDS             1337..1573
                     /locus_tag="ACG8M9_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379211.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1580..3532
                     /locus_tag="ACG8M9_002505"
     CDS             1580..3532
                     /locus_tag="ACG8M9_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0379212.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3604..4101)
                     /gene="dfrG"
                     /locus_tag="ACG8M9_002506"
     CDS             complement(3604..4101)
                     /gene="dfrG"
                     /locus_tag="ACG8M9_002506"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0379213.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1406 a          720 c          711 g         1278 t
ORIGIN      
        1 aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg
       61 atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg
      121 gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt
      181 taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac
      241 tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta
      301 tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa
      361 ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg
      421 agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat
      481 gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat
      541 tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt
      601 ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca
      661 tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac aattttgggt
      721 ttccctaaca catgatagga tctcacaaca aattgctttc tattattata ataacctgta
      781 acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc
      841 aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta
      901 cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat accattatac
      961 gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat
     1021 tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa
     1081 agaggcagca gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa
     1141 cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg
     1201 tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga
     1261 aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa
     1321 aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta agtctgtgga
     1381 ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat atgcagagcg
     1441 tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata agaagtttat
     1501 tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga
     1561 tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg aagatgaaat
     1621 tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta aacaatatga
     1681 tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct tgccaacgtc
     1741 ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca atccgttttg
     1801 ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac caagtcgata
     1861 taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca atgacaatcc
     1921 tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt ggtctgttgt
     1981 tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc cagattatca
     2041 atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc cgaagcaatt
     2101 ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa
     2161 gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac aaaagaacac
     2221 cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc gtacttgtga
     2281 tattttagga attggagttg gaacctacta tcataagctg gagtggctgt ataggcgttg
     2341 tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat ttaacgagat
     2401 gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga aaggacaagg
     2461 ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag ttgtatctgc
     2521 tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga atatttcaat
     2581 ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata cttttagtcg
     2641 taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta acgatagcga
     2701 aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta gatttgtcga
     2761 tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa ttaaacaaat
     2821 ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta gaaacgcctt
     2881 ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta tttgccaagt
     2941 taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa aagcagaact
     3001 gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt tggcatctca
     3061 ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta gtaaagatgg
     3121 agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca ctaaggataa
     3181 aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa acgaaatcgc
     3241 aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac agataagacg
     3301 ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga agagctatat
     3361 ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa gaacttacta
     3421 caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt
     3481 agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat gacaaaaaga
     3541 gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca attaacgcac
     3601 ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat acggattttt
     3661 atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat tcacttctgg
     3721 aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt tctcaacata
     3781 agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt cttcgttttt
     3841 acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa aggtaaaccc
     3901 cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga ttgattcaag
     3961 gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat attcccagtc
     4021 cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct tatccatcgc
     4081 agcaatcaaa gaaactttca tttataacac agcct
//
LOCUS       DAZKNM010000187         4086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000187 DAZKNM010000000
VERSION     DAZKNM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.193"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2782)
                     /gene="brxC"
                     /locus_tag="ACG8M9_002507"
     CDS             complement(<1..2782)
                     /gene="brxC"
                     /locus_tag="ACG8M9_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0379214.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYF"
     gene            complement(2807..3382)
                     /locus_tag="ACG8M9_002508"
     CDS             complement(2807..3382)
                     /locus_tag="ACG8M9_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0379215.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3382..3981)
                     /locus_tag="ACG8M9_002509"
     CDS             complement(3382..3981)
                     /locus_tag="ACG8M9_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0379216.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1224 a          783 c          551 g         1528 t
ORIGIN      
        1 caaaataaga gcgtaaattg tctaattgac cttttaaata tttcttgaaa ttttccattg
       61 tatgttcatt gttattttca tcaacaatat tacgactttg aaacagttct ctaccgattt
      121 cgtcaaccaa tttttttgct tttggcgaaa tcaccgcgct tggttcaatt tttatctttt
      181 cactttctcg tcgtttttga ataatcatta caatactatt tggatcatcc atccgtgaaa
      241 tttgtacatt gttataaagt aaatcaattt taccagacat gaaaagttta gcgatgcacc
      301 attccacatc aagttctgtg aatccatacg gttgttttgt ttcaaaacga tcacgaattg
      361 ttttcatcgt aatcgttagg tgatttctct tttgatcatt gataaaattg aaaatttcct
      421 gaactgcttg ctcatttgat ggatcttcaa taatttcatt tttgctttct ttcactaaat
      481 ataagatatc ttctttttct ttaggcgcat caatttggtt taatttatgg tatacttctt
      541 cagccatttt ctgaagtgct gattggaatt ttgctttaaa gtcggagcct gtcagttgaa
      601 tttctgcacc gttatagaaa aattttgcag atttcaacag ttcatttaat tgatcgttga
      661 tacttgcact tagttcctgt aattcaccag atttttccga acgaatcgcg tcaaagttct
      721 ttactacact cgaataagtc gttgttaaat aaagattcat tctagcatac attgctatat
      781 catctttaaa ttctttatct gatggcaatt ctacgaataa taagttacgg gaactcttaa
      841 gcgcaagttc tgcctcttga cctgctaaat cagagtatgg tgaaataaaa ttaactgtca
      901 tttcagcatt tccagcttgc ttgtatggac gttgatctaa cacttgtgca taagtaaacg
      961 tataccggtt tttaaattta tttccacctg acatgcctaa tttttcatag gcaaaactat
     1021 ttcggtctag aacgccgtca aaaattcgtt tagatagttc tacgatcaaa tcactatctg
     1081 taatattttg atttttaatc tcacgagtaa tttcctgttc ttcatcagtt tggaaaatat
     1141 aactatctaa gttcttccgc acaaagtttt ccttaattaa aaggtccaat gcttcttcta
     1201 ctcgtttttt agtttccagg cggtcttcgt tgatatttct tatcatcaag ctcgtaatat
     1261 tttcaacatt tggtttgatg gcttgatcaa catatttaat catgaataaa attcgtaaga
     1321 catttacgtt aaagttatct atatcagttg gtctttgaat atgagaatta tttgtagctt
     1381 gtgtaatcac tcggctaatt gctgtatcaa tccaacgttc taacgagtaa taaaactgat
     1441 ctaaagaaac caaactggtt gtatcatttt ccttatttcg ttcagcggct tctttaaaca
     1501 ttgctagtaa tgaacgttca ccagacgata agtttgatcc ttgatagctg tgattacgaa
     1561 tctcggttag aatttgcccc aataaattga attgataggg aataaatgga taaacttctg
     1621 cgtaattttc agcatcttgg aagagcttta attcaggact atcaacaaag agtaatttat
     1681 ttttcaattc tgtttgaaca gtttcaaatt gtaaggctaa agaatcattg gcaaactctt
     1741 ttttcttcaa aatacgttct ttaattactt catcggcatt tgctgaagat aaactcacag
     1801 ttgtggcaaa acgatccttg atcttagaaa aatcattatc agaattgtta tcaaagtttg
     1861 ttacgtgatc aatcgcttcc tgacttgtca ctaataccca agccttacct tcacaaattc
     1921 gacccaaatc ttccacaacg gtttgtaact gcaaagtcaa ctcacgatta ccgccgatat
     1981 attgtccgac ctcatcgact agaaaggcaa tatgatggtc tgcttctttg gaatcaagat
     2041 agcttttaac tagatttgca aagtcatcaa tgcttattaa ataatttcct gatgcttttt
     2101 ctaaaaaatt atcaatagcg gcttcactca taaaatcgat tttaagtgcc gcttctttga
     2161 tatcatcttg gatccaatca aactcatggc gctcttcttg ccattttgcc ccattaatct
     2221 cttcaaaagc agctttgaat gcatcaaact tttcttcttt aattaaccga cgttcaaaat
     2281 ctgctagatg tggaatcgtg cttaatcctt gcatttcgtt aaataccttt aaaaaaacat
     2341 tcacgattcc gtttttgtct tttttaccac ctgcatcact ttttgaatca atattaaaca
     2401 gaataacatc tgtatttatt tgtgagcacc gttccatcgt tgccaaagtc agtggatctt
     2461 tgattttccc atcttctttg aagtaatcaa tggctttctt gtcatttatt tctcgatttg
     2521 cgagcaagta agaaataatt tttaagaaat gagatttacc tgaaccaaag aacccctgaa
     2581 tccaaacacc cattttatct gttggtgtat tgatactccg ttggtaggca tcaaaaattg
     2641 ttttaaagtg tttttgtagt tctttcgtaa ccacgtattc ttctaattct tgttgaacat
     2701 tttcatcctt tgcttgaccg accttaataa ctccttgaat atttcggtca attggttttg
     2761 caaaaatatc tttaattagc atttaatctc ctaccttcca tcccaactaa attctaaatg
     2821 ctcgataata attatcatct ttattggtat taagaattgt caaacttaat tcatcatatt
     2881 cccctggata catcatcaca actggtacgc gatcaaatac ctgatgcatt ttatttaaaa
     2941 tagagtgcgc tctaattaat ggatataatt cgccaattcc tgtcaggaac acaactgatt
     3001 tttctggtgt atgggcatcc acatattcaa aaaatacatt ttcttcctca ggtaaatcca
     3061 ttttcaataa acgaccaatg ctttccacta atttttgtaa accacgtcgt tcttccatct
     3121 taatcgcttt ttcagtgaaa ccgcgttttt ctagatactc taacataatc acatagagat
     3181 tataaacttt cacttcataa ttatcaccaa cagatgttcc agatatatca ttaatataat
     3241 ctcttacaat taactcgtct ttcggatcgt agtcaaagat ataatagcct acttcattac
     3301 ctaaaccacg attcaataaa aaatcttctt tttgaattac ttcattcaac ttttccaaat
     3361 gctcttgaat cgataatgac attataaaat tcctcccagt gctagtacaa tatctttacg
     3421 attcttgtca actaaccatt gaatcaatcg tttatctaat aatatcggag tcattcgtcc
     3481 atattctttt tcccctctga ctaatcctgt ttcaattaga ttcgtttgat aagcggcaat
     3541 aagccgttta atcgtataat ctttccattt tgaaatatct tctgaacttc tccgcttctc
     3601 tgtaaaaaaa gaacggtaat caactggtgt taattgcaaa tttcctttac gtagattttc
     3661 agcatatacc tcagtcataa attcattaaa aagatcatca atcataagga tggaaatcaa
     3721 attcactaac ttttgattat ctatatttaa ctgattgaaa agttgcaatc cagcttcatc
     3781 cagttgacta actctccttt tcacgttctg atagatttct ttttgacggt tactgctcac
     3841 tgcaaaaaaa atattttcat gttcattttt ctctttaatt tctatatctg tatatccttc
     3901 tagtcgtaat tgaatatata acttgaactc tgaaaaccaa aagcgtttag aaactagacc
     3961 agcagaatat tttttattca atagattgcc tcctaatcat ctataattca ataagtaaac
     4021 aagtgttttt tgctcatcgt tagcattcat tttttagtat ctactataca atacatattt
     4081 attata
//
LOCUS       DAZKNM010000188         4074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000188 DAZKNM010000000
VERSION     DAZKNM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.194"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1769
                     /locus_tag="ACG8M9_002510"
     CDS             6..1769
                     /locus_tag="ACG8M9_002510"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0379217.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            1853..2311
                     /locus_tag="ACG8M9_002511"
     CDS             1853..2311
                     /locus_tag="ACG8M9_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0379218.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            2420..3103
                     /locus_tag="ACG8M9_002512"
     CDS             2420..3103
                     /locus_tag="ACG8M9_002512"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0379219.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3250..3864
                     /locus_tag="ACG8M9_002513"
     CDS             3250..3864
                     /locus_tag="ACG8M9_002513"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0379220.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            3880..>4074
                     /locus_tag="ACG8M9_002514"
     CDS             3880..>4074
                     /locus_tag="ACG8M9_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379221.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1434 a          669 c          863 g         1108 t
ORIGIN      
        1 gtattatgag tacagtatta catgtattgg atgcaatcaa tagccccgca gatgtcaaaa
       61 aattatctgt tgaggaaatg aaacagttgg cagaagagat gcgccaaaaa atcttgcaaa
      121 aggacagcga tgtaggtggg cacgttggtc cgaatctagg tgtagtagaa ttaacgattg
      181 cgtttcatta tgtatttgat tcaccaaagg ataaaatcgt ttgggatgtt tcccatcagt
      241 cttatggtca taagctttta actggtcgaa aaaatggttt tgaaaaagga cattaccgtg
      301 aagtgagcgg atattccaac caacatgaaa gtaaacatga ctattttaca gtggggcaca
      361 catctacatc tatcagctta gcaacaggtt tagctaaagc gagagatctg aagaaagaat
      421 caggaaatgt ggtagcattt atcggtgacg gctccctaag tggtggctta gcttttgaag
      481 gattgaacaa tggtggtgtt ttacaatcca atctaatcgt attagtaaac gataatgaga
      541 tgtctattga tgagaatcat ggcagtttat ataaaaactt agctgagctt cgtcaaacca
      601 acggtaaaag cagcaataac ctatttaaag caatgggatt tgattatcgt tacgtggata
      661 atggaaacga tctggaaacg gtgatccgtt tatttgaaga agtaaaagat attgatcatc
      721 cgattgtact tcacgtccat acggaaaaag gtcgcggtta tcagccggca atcgataata
      781 aaatgaaata tcattggcat gtaccatttg atctgtccac cggggaggca aagcaagtaa
      841 cttctggaga aacatttaat atgatcatca tggattattt ggataagaaa atgcaaacaa
      901 gtacagctcc tattgccgca gtcaatgcag cgataccagg cgcctttgga ctgaaaacat
      961 tccaagaaaa acatccagat cattattttg acgtggggat cgctgaacag cactctatca
     1021 ctttcgcttc tgctttggct tctcagggag tacgtccagt cgtcttccaa tcaagtactt
     1081 tcttgcaacg ggcatatgat caattatcac atgatttagc gatcaacgag aatccagtga
     1141 ttattttagt cggtggcgga acgatctcag ctagtgaccg aacacaccaa ggaacatttg
     1201 acattcctta cttatcaagt atcccgaaca tcaaatatct tgctccagca acaaaagagg
     1261 atatgatctc tatgctagat tgggcgttcg aacaaacagc gcagcctgta gcaatccgaa
     1321 ttcctacaca tggtgtagaa catggtacag ctatcgcaaa tgattatagt attccagcct
     1381 atcaaacagt aaataatgga ggaaaagtag caattttagc tttaggcggt tttcttgaat
     1441 taggaaaaga aacagtaaaa gcttttagaa aaaaaggaat cgaagcaacg ttgatcaatc
     1501 caggaacgat cacagaaaat gacgtagaaa cattagataa attaaaagaa acacacgaat
     1561 tagtcatcac attagaagat ggtagtttgt caggaggatt tggcgaaaat atcagtcgtt
     1621 attatggtga ttcttcaatg aacgtattaa attttggtgc agagaaagaa ttcacggata
     1681 gcgttcctgt agggcagctt tatgaacgtt atcatttaac accaaaacaa atcgttgaag
     1741 atagtctgcg tgtcttgaat attcaataat tcattatgct caccatccag ataattgttt
     1801 atactagtat ctggatggtt ttttcattat aaaaagcaaa ggggtggatg ttatgtatca
     1861 gcaagtagaa gaatatgtca aaaagttcca agaaaaatat cctagttatc aatcagaaaa
     1921 attaggtttc ttttcatttg gggatacaga aaaaatggca gacgagttga gcgaactagt
     1981 tgtaaaaaag ataaagactg gtacatcaag tggttacgat ctgtataaac aagagaatga
     2041 gcctttacca aaagttggtc aattagacgt ggtcctgaat ggaaaaaatg aaccagtttg
     2101 tatcatcaaa aatataaatg tgaccatctt gccttttagt gaagtcacga aagagcatgc
     2161 ttggaaagaa ggagaagggg atcggacatt agcttattgg cggaaagtcc atgctgagtt
     2221 ttttaaacat gcttataaag aagcgttgaa catagaattt acagaagaga agctggtcgt
     2281 atacgaagaa tttgaagtaa ttttctttta gtaagaaagg tctgctgaaa gaaatagctc
     2341 attattgtct cctgaataga aaagggttac atttttatat gaagctgtat ttttatataa
     2401 cattactgga ggagatattg tggatacact ggaacaaatc aaaggcggag tcgtcgtttc
     2461 ttgtcaagct ttggaaaacg agccgctaca cagttctttt attatgggga gaatggcttt
     2521 ggctgcaaaa gaaggcggag cagttggtat tcgagcaaat acgacagaag atatcaatga
     2581 aatcaagcga atcgttgatt taccggtaat agggattatc aaaagagatt acgatgggtc
     2641 agaagtgttt attactgcga cagaaaaaga agtggaagaa cttttggaga cgcaagcaga
     2701 aatcattgca ctggatgcaa cttctcgtaa acgtccgaca gaaaaaacaa ctgctgaact
     2761 tgtccaaatg attcacgaac acggtcggtt agctatggca gatatttcga cttttgagga
     2821 agcgcttcaa gcgcaagaag atggttttga tcttttgtct acgactttag caggctatac
     2881 agaatattca cgaaagacgc agacacctga ttttgattta ttgaatcaaa tcatagaaca
     2941 agtcgatatc ccagtaatta tggaaggaca tactgactct cctgaacaag tagagaaagc
     3001 atatgaatta ggtgcttttt cagtcgtagt tggatctatt atcacccgtc cgcaattgat
     3061 cactaaaaga tatgtcgaag ccgcaagtaa agcaaataac taaaaaacac gcacggcaca
     3121 gattcttgac aaaaaagaaa aattgataga tagtttaaag cagattctaa gaagatgaaa
     3181 aaagcattac ttttcatttt gtcttcaaga tctgcttttt aacgaatttt atagaaagaa
     3241 ggcgaaacca tgtttgaacg gagtccttca gaattgacag aacgtgaaaa ttacaagttg
     3301 ctgattggtt cagtcatccc tagaccagta gcagtagtaa cgactaaatc agcaaaagat
     3361 atagtgaata tcgcaccctt cagttacttc aatatcgtca gttcgaatcg acccatctta
     3421 tccttggcga tccagagaaa acaagatgca gtaaaagaca cagcaaagaa tattcttgag
     3481 acaagagaag ctgttatcca tatccttgat gaggacaacg tagaagaagt aaataaaaca
     3541 gcagccaatc tgcctaatga ggaaagcgaa ctgtacctga ctgacttgac tttaactgat
     3601 tcagtaatca tttcagtacc agcattgaca gaagcaaaag ttcgttttga aacatcctta
     3661 tatgaacatg tcgagatacg gaataaaaat aaagtaacag ctgatttatt tttgctagaa
     3721 gtacagaaat atcatttaaa tgaaacagtc tatgatgata agacaggaag gatcgatcca
     3781 agaaaattga atgctgtaag ccgtttggca ggaaatgatt atgcagcaat aggagaaatt
     3841 ttcacgattg cacgtcccga ataaaaagga gcactagtta tgttaaaaat aagctataaa
     3901 ccatcgacag attcaaaaga aatgaaaaaa gaatatgaaa cagtgaatga ttttctgcaa
     3961 gggcagtact tagaagttcc gccattgcaa gaccattttg tcgttactac tgttacttta
     4021 gatggtaagg aaatagaaat gccagatcaa acgattagcg gattgtttaa ttac
//
LOCUS       DAZKNM010000189         4068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000189 DAZKNM010000000
VERSION     DAZKNM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.195"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..1451
                     /locus_tag="ACG8M9_002515"
     CDS             27..1451
                     /locus_tag="ACG8M9_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0379222.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1632..2405)
                     /locus_tag="ACG8M9_002516"
     CDS             complement(1632..2405)
                     /locus_tag="ACG8M9_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0379223.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2402..3307)
                     /locus_tag="ACG8M9_002517"
     CDS             complement(2402..3307)
                     /locus_tag="ACG8M9_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379224.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3461..3781)
                     /locus_tag="ACG8M9_002518"
     CDS             complement(3461..3781)
                     /locus_tag="ACG8M9_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0379225.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1222 a          754 c          816 g         1276 t
ORIGIN      
        1 gcggttttat tttggaggat gcttacatgg atacagagca acagaaaaaa ggaattggtt
       61 taattccact tgctgcctta gtaattggtt cagcgattgg aggtggcgta tttggtatta
      121 tgacggacat ctccggatcg gctggcggcc cagctatttt cagttgggta ctcgtaggaa
      181 cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt ccagatttaa
      241 aaggcgggat ctttagttac gcagaagaag gttttggccg tttcgctggt tttatcagcg
      301 gttggggtta ctggctcaca tgctggttag gcaacgtagc ttttgcaaca ttgttgatga
      361 gcgcattagg atatttcttc ccagtatttg aaggtgggca aaatctggca tcgatcgtga
      421 tgagtacagt ctttttatgg ggatatgcat ggctagtgaa tcggggagtc gaaaatgcta
      481 gtttcgtcaa tgctatcgtc acattctgta aattagtgcc attattttta tttattgtag
      541 tagctatcag cacgttttcg atccaacaat tcattgataa cttttctagc gacattatat
      601 tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt attgagttgc atcatggtga
      661 tgctttgggt atttgttggt attgaaggag cctctgtcgt aggttcacga gcaaaaaaac
      721 aatcagatgt tgggaaagca acgattctag ggatattcgg cttgattatt atttacgtct
      781 tggtttccat gcttccatat gggacaatga cgatgaatca gctgcaaacg atcaaggcgc
      841 agcctgctat gggctatgtg tttgaaatga tggttggtaa atggggagca gtcgtgatca
      901 acggcggttt gatcatttct ttagttggtg tttggctctc atggacgatc ttaccgattg
      961 aaacaatggg aaacatggct gaagacggac ttttgcctaa agcatgggga aaactcaaca
     1021 aaaatggggc accaacttat gctatcgtat tgacgacgtt atgtacgaat gtctttttac
     1081 tttcattatt attcactgac agtgcttata actttgctta cacacttggt acagcagcta
     1141 ttttcttcac gtggcttttc ttaggattgt accaaatgaa actttcttat cagcggaaag
     1201 aatgggtcca atttttagta gggcttttag caagtgcctt tcaagtatgg gctatgctag
     1261 tcgtagcctt taatgaagta atgcttgttc ttgttctttt cttgccaggc atttattttt
     1321 acctcaaagc acgtaaggag caaaacgagc ctgtacaaca tatccactta gataagattt
     1381 tacttatagg tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc actgggatgc
     1441 ttcacgtata gaagtataaa agaagtataa agagaggttg tgagcctgtc gtttattcaa
     1501 aaacatacta ggaaaggagc tgtgacacaa atgttgtcac agctcctttt tcagaataaa
     1561 cggtgtttaa gagacagcca atcggcaata aggctgaacg tctctttcac agcctctttt
     1621 ttgatgagtc atcatacatt ttttcgctta aatacgagat atcctaatgc taagaacaaa
     1681 acgatgtaag ctaaattacc gaatagcatt tgattagtag aaagatgagt caattgcttc
     1741 atgatctctt cgtttccaat ttggttttgc agattcaaca tactgatcgg attccatttg
     1801 atccactccc atttttggat gagtgcaata aggataccag atatgattga cgaagcaaaa
     1861 taaaagacga ttcctgctga gatggatgcg ccggaactat tgataaagca agcaatcatc
     1921 aacactaaac ttaaaatcag ccataaaccg atataactac ccaaagtata agtgaacaag
     1981 ctttggatca gtgtttgacc agtagaaacc ttctccgtga aggaaatcga agggaaaaga
     2041 atcagtttca tcaacacagt gacaataata gctaaaaggt ataaataaac agaataaata
     2101 actaatgtga tccatttgct tactaatatt tctcctcgag aatactttcg ataaaggaga
     2161 tttttcaatg taccatattg tgcttccatc gaaatgatcg tgcttgcagc tgcaatcatg
     2221 ataaagacaa tccaagatgt ggcactaaaa agctgagcag tcatttcaac tggatcaatg
     2281 atcgtcgtgt attttttggc tagaagtgct gttcctatga gcaaaacaac taatagaaca
     2341 gataaaacag cagttgactt tttcttgatc aatttaaata attcttgttt tacaagcaat
     2401 gtcatttttc ttcttccccc tttactgaat ccagaatttc caatagagaa gattccaaat
     2461 cattcgtcag atgctggacg tctgtcacat ggatattttg tgcagacaat agctggatca
     2521 cggttcctaa taattcttcg ttttctttta aaagcttgat tttatcagga tcttccaaag
     2581 tatatccagc ttctgtcaag atcgttcgag cagctagatc atttgttgta gagataacga
     2641 tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg atctttcctt
     2701 gattgatcag gatcagctca tcgaccattt tttcaagctc gctgagtaag tgactggaga
     2761 tcaagaaaga ggtgccctga gtagccaagt cggtaatgat ttttcgcaca tctcttgttg
     2821 cttgtggatc caaaccgttc atcggctcat ctaaaataac gatagacgga tggttcagta
     2881 aggcaagagc aattcccaat ttttgtttca ttcccaacga gtaggatttc gcttttttgt
     2941 gaatgtactt ttccatcttg agctgattca cgatagtgtc gatagcagat acatcgttgg
     3001 tttcagagaa taacttcaga tgatcatagc ctgttaagaa tggatagata cccgggtatt
     3061 cgatcaatgc tcctacttgt tccaaccctt tatgactaga gggagaaatc tcattttcac
     3121 caatacagat tgttccatct gagtaatgga tcagtcctaa aatagctttc atgatcgttg
     3181 tttttccagc gccattagga cctactaatc caacgatttt gccaggggat aagctaaaac
     3241 tgacatcgct gagggcttgt ttgtgaccaa agttcttgga taattcttgg accgaaagac
     3301 ttttcaaaaa tattcactcc ttttctatag atagtacaag catacacctg atgagtggag
     3361 aagtcgctag gtcattggac ttatttctta ttttttcata aaaaaattga atattaaata
     3421 aaaaggctta ttttttctga tcagtactta ttccaagtgt ctagtggccg ctagtaatct
     3481 tgatgccaat gatactgatg agtagcatcc ccacaaagaa ccaagtggaa agattcagct
     3541 tatcatgaaa caagaccaca ccaattatga ccgaaccaac tgcgccaatc cctgtccaga
     3601 taggataaga gagactcatc ggcaaacttt tgattgcctt tgacaaaaag taaaaactag
     3661 caatcatgcc aatcactgtg taaaaagaat aattgatttt agaaaaacca tcactccatt
     3721 tcatcatcgt tgaccagaat acttccaaaa ttcctgctat gattaattcc atccatgcca
     3781 tacttacatt cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc atcccttcaa
     3841 agacctaatg gaatgatcat cctctttatc ctacccggtg gcagattgta tatctttttc
     3901 tgtagtatag cacacctatc cagatatcgg caatctcata ctttttagta aagtgtacca
     3961 agcactgacg gatatatcgt tagcggaaaa gaagatgaat gctttgcaat ttcatagaaa
     4021 gaagttgtta tttattaatc atttaaaaaa aacagtaact agacctta
//
LOCUS       DAZKNM010000190         4065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000190 DAZKNM010000000
VERSION     DAZKNM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.196"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1348
                     /locus_tag="ACG8M9_002519"
     CDS             47..1348
                     /locus_tag="ACG8M9_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0379226.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="ACG8M9_002520"
     CDS             1375..1635
                     /locus_tag="ACG8M9_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0379227.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="ACG8M9_002521"
     CDS             complement(2634..3869)
                     /locus_tag="ACG8M9_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0379228.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1156 a          660 c          851 g         1398 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gattt
//
LOCUS       DAZKNM010000191         4056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000191 DAZKNM010000000
VERSION     DAZKNM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.197"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..899)
                     /gene="uxuA"
                     /locus_tag="ACG8M9_002522"
     CDS             complement(<1..899)
                     /gene="uxuA"
                     /locus_tag="ACG8M9_002522"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0379229.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSL"
     gene            complement(902..2533)
                     /locus_tag="ACG8M9_002523"
     CDS             complement(902..2533)
                     /locus_tag="ACG8M9_002523"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0379230.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            2837..3508
                     /locus_tag="ACG8M9_002524"
     CDS             2837..3508
                     /locus_tag="ACG8M9_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0379231.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(3668..3859)
                     /locus_tag="ACG8M9_002525"
                     /pseudo
     CDS             complement(3668..3859)
                     /locus_tag="ACG8M9_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1318 a          756 c          558 g         1424 t
ORIGIN      
        1 tctagagaac catattctga tttatgcggc gcttcgtaaa aatcgagtcc attgtcactt
       61 aaatatttga tattacgaac atgtgcaaaa ggaatccgat cccgttgaca aaattcagat
      121 agaatttcat agacatcatt atctggtcgt tctgcaatac ttccagtaca taatgtaata
      181 ccattagaag gagaatccac tacattacac aaccaatcta aatcgtcacg atttttaaca
      241 atacgaggta atccgaaaat ggaatatggt ggatcatctg ggtgaactgc cattttaatc
      301 ccaacttctt cacatgtcgg gataattgct ttgagaaaat atgcaaagtt ctctcgtaat
      361 ttctcttcat caacttcttt ataggcttct aataattgct taatttgtga taaccgatga
      421 ggttcccaac ccggcaattc aaaattatta gtagaatttt ctacttctct aataatttcc
      481 tccggatcag tagggatatt ttttgaaata taggctaacg tagaagaccc atcatctaaa
      541 cgataatcca aatccgattt tacccaatca aatactggca taaaattata acagataacc
      601 tcaatcccaa attctgccaa attgcgaatc gtctgtttat aattctcaat gtattgatct
      661 cgtgttggta aaccaatttt gatatcatca tgaacattaa cactctcaat aactttcaag
      721 gtaagtccat tggcttcgac ttcatctttt aaagatttaa ttcgttctct cggccaaacc
      781 tctccggctg gtatatccat taatgttcca acaattcctt tcattgctgg gatctgacga
      841 atttcttgta gtgagatttt ttcttcattt gccccatacc atctaaaagt catttccata
      901 gttactttcc tccaacttct gttatatatt tattgattgt tttacgaact gcttgaggac
      961 ctactagcat ttcgcaaaag attttttcag ttttctttcc taccccaact tgatataaat
     1021 ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac cgcacaatga atctttgaag
     1081 gatcttgttc acctaatttc aatactgata atttatcctg caactctacc aataacggat
     1141 caggacttgg cacaaaattt tccccttgat catcgactgc cagtaaataa cgtaaccaag
     1201 cagcaatagc gataggaata aatgttagat tttctgcatt tccgtctttt tcgacatact
     1261 ttttaattgt ttcaccatag cgaatcgcta ctttttgaga agtatctgtt gcaattcgtt
     1321 gtggtgtatc tggaatatag ggattaggca aacgtttatt aattacttca tcaataaatt
     1381 gttttggatc aataatgcct ggattttcaa ctactggtaa accttctaaa taacctattc
     1441 ctttgattaa accaaccaaa tcatcatcct gcatctctgc tgaaattgat tgatatccta
     1501 gcagacaacc tgttactgcc aaagcagtat gaagtggatt caagcttgtc gtaaccttca
     1561 tgatgtctgc tttatcaacc gtctctctat ctgtcataat cacaccagcc ttttccaatt
     1621 taggccggcc atttggaaaa ctatcttcaa taactaaata atgaactacc tcagtattag
     1681 tgaaagcagc aatattggta tgtttttttg tgtgaatgat atctaaatcc tcgagcccca
     1741 tcatcaatag atcttccttt acattctctg aaggatttgg cgtaattcga tcaatcatac
     1801 tccaaggaaa agtcactttt tctttatcct ttaaataagc aataaatttt tcagaaacat
     1861 atccttcttt ttgccaagct tctgcaatcg taaaaatact atcttgaaaa cgttgaccat
     1921 tctgtgagaa attatcagta gaaaccatcg caatcggcaa ctctccagca ttaaatctgc
     1981 tatataacaa ggatgttaca atactcatcg tatgttgagg attgctaggt ccagatttga
     2041 tatcagcttt gactaccgaa aagaattgcc ctttagaatc ttttaaactg taaccttttt
     2101 cagtaatagt aaacgttgta aactgtaatt taggatcttc aaaatattta accactcttt
     2161 gatagtccgc tatattcttc ggattgcaat ataaactatc agccgttact gccaaaagtc
     2221 tttttttgag ttcaccatct ccactcataa caatttccaa aatgtcattg ttatagtcat
     2281 gataagcttt ttccactact tcttcatcaa atgtttcaca cacaactact cctgtttcta
     2341 attcattttg atctgctaag cattgagcaa tttctgcatg aaatcctcga taaagatttc
     2401 ctccaccaaa atgaagccat actggatgtt gctggccttc ttgcttaact tctttgacat
     2461 caaaacacgg cagtagaatc tgcatttcat tgaattcttc gatagaattt tgccaatttt
     2521 taatcgaaac cataattaac ctcctcaaac tgatatgtta gttttgatac attttatatt
     2581 ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat aaacttgctt ttttctacca
     2641 tatcagaatg attgtatttt ttattcagaa aaataaaata ctccttcttt ttggactgat
     2701 tttatcgaaa cttatttttt ctttttttca tgtattcttt tatttttata actagtgtat
     2761 tacttcaaaa tttgatatta tatggcatga acagaattaa aatatcagtt aaaataaaag
     2821 ttaaggagca ttgtgtatga caattaaaac tagtctccaa aatcaagctt accaatctat
     2881 tcgccgacaa attatctatg ctgacttaga acctggaaaa aaagtatcag aaaaaaaatt
     2941 agaagaaaca ctttctattg gtcgtacacc aatacgtgaa gcactgattc aactccgaca
     3001 acaaggtctt gttgatacaa ttcctcaaag cggaaattat atctcattaa ttgatttaga
     3061 ttcggccaaa aatgctcgat ttgtcagaga acatttggaa cgacaaatta tgttagagtg
     3121 ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa actataattt cagaacaaga
     3181 aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat aatgataatt tattccataa
     3241 agcttgtttt gaaattgcag agcgagaaga agtatggcaa tggttagaaa ataacaatac
     3301 acacttagaa cgatttcgat ggctacgagt aaaaacggaa ggattaaagt gggaaaaaat
     3361 tatggaacaa cattatcaac tatttgaggc attaacaaaa caagatccag aagaagctaa
     3421 ctttctaaca tcgttgcatc ttcatttaat gcttaatgag caaaatattg ttttagctac
     3481 tcatccaaaa tatttcaaaa atatataact tttaactctt tttaaattaa atggttgttc
     3541 tgatcagaaa aatatcgctt gttttcttat tatgtaatat atttcataaa ctattctgca
     3601 taacaactat tgtttgtact ttttacattg gataactaat tatgactgct gataactttt
     3661 gatattaaac tagtatctcc aatacaaata aaaaatagat atcttgttca tcaaaattag
     3721 ttcgtggata cttaccttca tttacatcat tcagcggttt cccattttct tttattctat
     3781 tatttcattt tctaatagac ttggcttcat ttcgcaatac tttttttcaa taggcatcaa
     3841 attggtgttg tttagatatt tagttgtagt tttgtgacat gataagtatg ctcctttttc
     3901 tcttttccac taatacaacg atcaaagggt gcttttttca aaaaactaga aaaatcaaaa
     3961 taagattcgt attttgattc tatatatcct gtttcaacta catcttatat ttcacaatag
     4021 aataaatagc cttttgcata gctgcttaac ttataa
//
LOCUS       DAZKNM010000192         4034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000192 DAZKNM010000000
VERSION     DAZKNM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.198"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1648)
                     /locus_tag="ACG8M9_002526"
     CDS             complement(143..1648)
                     /locus_tag="ACG8M9_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0379232.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1645..2331)
                     /locus_tag="ACG8M9_002527"
     CDS             complement(1645..2331)
                     /locus_tag="ACG8M9_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0379233.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2524..3945)
                     /gene="gndA"
                     /locus_tag="ACG8M9_002528"
     CDS             complement(2524..3945)
                     /gene="gndA"
                     /locus_tag="ACG8M9_002528"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0379234.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1111 a          854 c          690 g         1379 t
ORIGIN      
        1 atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt tttttataat
       61 gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa
      121 aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc
      181 taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa
      241 ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta gctcttgcat
      301 tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc
      361 caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt
      421 cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa
      481 agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca
      541 catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt
      601 tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct
      661 cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta
      721 atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg
      781 caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat
      841 acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat
      901 ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg
      961 tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac
     1021 tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa
     1081 tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac
     1141 ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg
     1201 ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca
     1261 agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa
     1321 aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc
     1381 cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg
     1441 agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg
     1501 ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga
     1561 atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc
     1621 ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt
     1681 ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac
     1741 gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc
     1801 acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt
     1861 tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa
     1921 atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg
     1981 acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta
     2041 gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat
     2101 cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa
     2161 ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa
     2221 accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc
     2281 gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca tttattattt
     2341 tatcctttct attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa
     2401 aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag tctactcata
     2461 acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga
     2521 ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata
     2581 ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc
     2641 tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg
     2701 gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa
     2761 gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt
     2821 ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa
     2881 tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata
     2941 actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc
     3001 tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc
     3061 tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc
     3121 taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag
     3181 gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc
     3241 agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc
     3301 catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag
     3361 ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt
     3421 tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa
     3481 gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt
     3541 taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc
     3601 taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat
     3661 atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc
     3721 tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt
     3781 atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc
     3841 tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt
     3901 acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac
     3961 atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa
     4021 agaacaacat attt
//
LOCUS       DAZKNM010000193         4003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000193 DAZKNM010000000
VERSION     DAZKNM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.199"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(149..691)
                     /locus_tag="ACG8M9_002529"
     CDS             complement(149..691)
                     /locus_tag="ACG8M9_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0379235.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(696..923)
                     /locus_tag="ACG8M9_002530"
     CDS             complement(696..923)
                     /locus_tag="ACG8M9_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0379236.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1002..2882)
                     /locus_tag="ACG8M9_002531"
     CDS             complement(1002..2882)
                     /locus_tag="ACG8M9_002531"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0379237.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3071..3685)
                     /locus_tag="ACG8M9_002532"
     CDS             complement(3071..3685)
                     /locus_tag="ACG8M9_002532"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0379238.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            3827..>4003
                     /locus_tag="ACG8M9_002533"
     CDS             3827..>4003
                     /locus_tag="ACG8M9_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0379239.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         1173 a          846 c          639 g         1345 t
ORIGIN      
        1 tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt tgtctgtaat
       61 acatcagcgg gtgcttcttt tattttaatc ttcgtgattt tccaatttac ctcacgcttt
      121 atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca tccaaatctt
      181 gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta ttgaaactta
      241 aaagttcaac aagcaattgt tgcaagccat atttttcttc tttttcagct aatttgattg
      301 cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc aacattgttg
      361 aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc atgacttctt
      421 ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt ttattgaacc
      481 atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc gaagatgtac
      541 ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa tttgctaata
      601 catgccccac catggcgcca gcagttggtt tatgatggtc gatatctgct tgttttgttt
      661 cttgaacaaa tttttcttcc actatattca ttgatttacg cctcttttac ttttgctgat
      721 tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg gacttcatct
      781 ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca
      841 ggctgttgag aaaccgcatg ttcgattttt tgcatacagc ttggacaagc tagtgtatct
      901 aattgcataa taattttttt catcgtagtt cttccttcct ttttcttgat agtcttattc
      961 tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt ttcttctttg
     1021 ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa gatactagct
     1081 tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc gttgagtaaa
     1141 aataagacga caccaatggc aatcactaca ttttcacggg tatttaaaac agttttctta
     1201 gccaagcggt aggcatgaac caacgattca aaattagatt gcatcaagac aacgtcactc
     1261 gtttcgatag caacatccgt tcctgatccc atagcaatcc cgatatcagc cgcagccaaa
     1321 gagggagaat cattgattcc atctccaata aaagcaacgc gcaatccttg ttcttggtat
     1381 tttttcacaa attgaacctt ttcgtctggt aataattcag catggacttc atcaatgcct
     1441 aacatatcag ctacataatc agcagtcatc tgattatcac ctgtcagcat cactaactgt
     1501 tttgctcctg cttgtttcaa cttagctaat tgttcagcga cttctggtcg aatcacatct
     1561 gacacaccta tgatttgagt gatttttttg tcgattgcta caataatgac cgagctgcct
     1621 gtttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag tttgtgagcg
     1681 gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc tgtgattcct
     1741 tgtcccttga ttgtctgatt atctttgact gctaattgtt gaaaatcaat gtctttctcc
     1801 tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc taatcgagcc
     1861 actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga tacagcagta
     1921 gttcctttgg tcaatgtacc agttttatca aagacaaaag tatcgatttt ggaaaaagta
     1981 tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc gattcctgct
     2041 acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac caaaacagtg
     2101 attgctaaac gaaaatcacg actaatcaga ccaacaacca acgcaataag taaaacggtt
     2161 ggagtataat actgagaaaa tcgatcaatg aatttttccg catgggattt ggtatcttgt
     2221 gcttcttcta ctaattcgat aattttggca aaggtcgtat catctccgac cttagttgct
     2281 tcaatcgtca cgtagccatc tgctaaaatc gttccagaga agacttcatc tccactgatt
     2341 ttgacgactt gtcgtgattc accagtcact gcggcttcat ccgcataccc tttcccgtca
     2401 atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat atcgccttct
     2461 tccacgtcat cgatttccac ttcttcagtc gttccatctt cttggacaac actagcagta
     2521 gatggggcca tctcggtcaa agccttgatt gactcccgcg ttttggtcaa tgttttttgt
     2581 tccagatagc taccaaacaa aaacaagaat gtaacgatag ctgactcctt gtattctccg
     2641 atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat aactttgact
     2701 ttcactgcct gatacgcatg gatcataata ggtatgaccg caataataga agctatggct
     2761 agagcaatgt tatagaacaa gacaaaacca gtaaattttc cgataaaacc aaaaataatc
     2821 aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt ttgtaatttc
     2881 atctttctcc ctcttttcct ttcattctta cgagactaag tatagatact tatccatcat
     2941 aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt agaatacact
     3001 acaaaaaata gttagcaaga agactcacta aaagtgatct tgccaactat tgtcgtttct
     3061 atcgaaattt tcattgaatg attttcctgt acagcataca tcggagatct atttctttat
     3121 ctaagatttt tatcttgcta attttttcct ttagtctttc aaatccacgt cgttcataga
     3181 attgatacgt acagccacta tctgtaaaaa ggaatatttc ttttcctttc tctctttttt
     3241 caaattctgt tagcaacttg gtaccgatgc cttttgtttt caaatcagga tttgcagcta
     3301 aaaaaatgat ctctccatct ggcgaatgct tctttaaata tgctgcaagc atttgttcat
     3361 ttgctgtctc gtaagctcct ttactttctt tagcaaatat attttgtaaa aaatcgaata
     3421 tcttaacata tatttgctgt ccaaaagaac gatgtttctt tttttctcct tttatctccg
     3481 ccagcaaaac acctgccaac tcgtcctctg catacaaagc aagaatttga gttgctcgat
     3541 tcaactccat atacaaaaaa tatcttccgt aaagattcag cagaagttca ttttccatat
     3601 atctctcaaa gttcattcct tcaacagcaa aatagatagc ttttttgtaa tctttctttt
     3661 ctaagggttt aaaagttagt ttcatcattt cgcctcctca acttcaatga ttgtgttata
     3721 cttacattat aatagacaca gtgtctacta tctataatca atcatctata agtttgtcga
     3781 ttaagcgaca gctttgcttt gatatgtcgg ataaggagat aggtaaatga aaaagacaga
     3841 tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat tattagtaaa
     3901 taaaaagatt caagcaatca ctatacgcga actaacagaa acggcagatg tacatcgttc
     3961 gactttttat acacattata aggatatcta tgatctatat gat
//
LOCUS       DAZKNM010000194         3991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000194 DAZKNM010000000
VERSION     DAZKNM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.200"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..353)
                     /locus_tag="ACG8M9_002534"
     CDS             complement(204..353)
                     /locus_tag="ACG8M9_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379240.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            815..1027
                     /locus_tag="ACG8M9_002535"
     CDS             815..1027
                     /locus_tag="ACG8M9_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379241.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1305..3104)
                     /locus_tag="ACG8M9_002536"
     CDS             complement(1305..3104)
                     /locus_tag="ACG8M9_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0379242.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3228..3824)
                     /locus_tag="ACG8M9_002537"
     CDS             complement(3228..3824)
                     /locus_tag="ACG8M9_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0379243.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1430 a          708 c          686 g         1167 t
ORIGIN      
        1 tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt
       61 ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag
      121 acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt
      181 cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg
      241 attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta
      301 gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc
      361 atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc
      421 aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta
      481 ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg
      541 ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa taacttagca
      601 atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg
      661 tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa aataaaaaat
      721 aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa aataaagaaa
      781 aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag taggaagatc
      841 ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg aacgtacata
      901 tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc aacttatcaa
      961 tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg ctgaattggt
     1021 ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa taaagacaga
     1081 agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat tattcttgca
     1141 gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga atcaagagaa
     1201 agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt ggcaggttat
     1261 gcgttttttg ttgtataaag acttttttca ttaacgattt ttgattagcc gactcccaaa
     1321 acaaaattta gcagtttatc tgaataagtg atttcttcat ctgtttgttt cacactttca
     1381 tttagtagtc ccatctcatc ggttatgata ccatccacac cataaaacat catgcgactc
     1441 atagcatctg aatcgttgat tgtccataca tatacttctt ttccgtcttg gtgggcagaa
     1501 ttaatgaagt tacggttgat cgtactatat tccattgtca gaaaatcagc aggcgtcaca
     1561 gggggcccaa caatattgaa aggtaagatg tagcccacat aaaagtttgg ttctgcttct
     1621 tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt ctctaagata
     1681 actttgcggt atttatggac aaatcgttcg acaaggtcat ccgaatcttt tttagaggtc
     1741 ttgatctcaa taagtaattt ttggtttagc ttatgtgctt tttccaaata agaatcaaaa
     1801 gaacaaacag gggcttgcac tccgttttca gtaactgtaa gatttgtcaa ttctttcaat
     1861 gtaagatcct gtggtgttcc atttacccct gttaagtgac gaagatttgc atcatgcatc
     1921 actacgaaat gaccatcttt tgtttcctga acatccatct ctacataatc aggatgatag
     1981 tctctgcttg ttttttcaag tgcttctaaa gtattttgcg ccccattttg ggtactcaca
     2041 cctctgtgag agacagtcac tggttttgtc tgaacagctg aagtcagata atccatattg
     2101 taaagacaga cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt gtttttcagc
     2161 gcagagaaac gcaagttggg ggcttgtggg ataaaccact tagggatctc aggcaagaag
     2221 ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc ctacggtaga catcacgata
     2281 ttgaataata aaatcccttg taaaagcgtc atagcaaaca cagcgctgat caatgcatag
     2341 tctgggaaaa attgttcaac catactttgt gccaaaataa caacgatata tcctaaagaa
     2401 gaaaggagta aaatcgtacc gccaatcaca ataaattggc ccgtgatggc caaaagacgc
     2461 gatttggtca atgaccagct ttcccgaatg gctgctcgaa aaggacgatc tcgtagaatc
     2521 atttccggta aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat ataaacgagt
     2581 aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa aatccataat gaaagcgggg
     2641 atttttattt ttgacagtag atcagaatta aaacttaatc cgctgatcgg tagaatcaat
     2701 aaaaaataag caaggaaaaa caaaaaagta atgggtctca ctttcttcag ctgaagtatc
     2761 gtcaagtgta agagttgctt caaagagatt ggttctttct ttttaataaa aaaaacactc
     2821 atcaaaagaa aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa aactacgatt
     2881 aacgagagta gaaccccagg atgttgagaa aaaattgaag gaatattgtc ataagacaaa
     2941 tagtcaattg cccctcggtg taaaataaaa cgggtcgtac tgctcaataa gggtaaacaa
     3001 ataaataaaa taaagccatg catcagcaag acatcacgga aataagcttg tgttcctttt
     3061 aaaaagtccc aagcattacc taagctacgt ttcaaataat tcatcctatc acctgctctt
     3121 cacattgtag cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg cagacccttt
     3181 cccagattta acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga
     3241 ccacttagat attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt
     3301 ttagcaatca aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc
     3361 aaacataacg catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca
     3421 aaacggtttt gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg
     3481 gaaagtatga atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg
     3541 cagattccaa tatagttata gataaatcca gcataagggc caaatatcag gacaccagct
     3601 gcagtactga tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca
     3661 aaaataatcg gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct
     3721 ttgaaaacac ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata
     3781 atggatatga aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt
     3841 agaggttctt tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata
     3901 tgacagaaaa caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa
     3961 acatccaact aaagacttat ttaataactt g
//
LOCUS       DAZKNM010000195         3982 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000195 DAZKNM010000000
VERSION     DAZKNM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.201"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..357
                     /locus_tag="ACG8M9_002538"
     CDS             <1..357
                     /locus_tag="ACG8M9_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379244.1"
                     /translation="ITLSYFLVKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKI
                     NCSHYHANGFDMGSIHKSSLLSPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIR
                     TANYQKKFKLKLKDSF"
     gene            complement(459..629)
                     /locus_tag="ACG8M9_002539"
     CDS             complement(459..629)
                     /locus_tag="ACG8M9_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379245.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(660..908)
                     /locus_tag="ACG8M9_002540"
     CDS             complement(660..908)
                     /locus_tag="ACG8M9_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379246.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(921..1106)
                     /locus_tag="ACG8M9_002541"
     CDS             complement(921..1106)
                     /locus_tag="ACG8M9_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379247.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            1410..1784
                     /locus_tag="ACG8M9_002542"
     CDS             1410..1784
                     /locus_tag="ACG8M9_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379248.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            1789..2217
                     /locus_tag="ACG8M9_002543"
     CDS             1789..2217
                     /locus_tag="ACG8M9_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0379249.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            2267..2920
                     /locus_tag="ACG8M9_002544"
     CDS             2267..2920
                     /locus_tag="ACG8M9_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379250.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            3056..3622
                     /locus_tag="ACG8M9_002545"
     CDS             3056..3622
                     /locus_tag="ACG8M9_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0379251.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            3738..>3982
                     /locus_tag="ACG8M9_002546"
     CDS             3738..>3982
                     /locus_tag="ACG8M9_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0379252.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVI"
BASE COUNT         1423 a          693 c          696 g         1170 t
ORIGIN      
        1 attacgctct cctatttttt agttaagcat gatagaagta atcgaatgat tcttactggc
       61 gtaattgcaa acccatcaaa aatgccaaac tcaattatcg aatgtacgta tctttacaac
      121 ggtaagaaaa ttaattgttc acattatcac gccaatggtt ttgatatggg aagcattcat
      181 aaaagtagcc ttttatctcc tataccgttg gcacaagcaa tctctcctgg ctactcagtc
      241 gctttttcag aagttttaga tatgaaagat atattacctg gtgaaaaact tgtaatggtc
      301 ataagaacag ctaattatca aaaaaaattt aagttaaagc taaaagactc attttaattg
      361 aaaaattttt ctttaataat caaatacaaa ttaattatta ttgatccaat cccacaaaac
      421 ataattaaca gaaatacaat atcgtcctta ctcattagtc agtccctccc gactggcttt
      481 tttgttttgt actcagcttc atccagccct ataaaaatcc aaaccatgta cacaatcgtt
      541 cctatcaacg cttgtctact tccccaaagc cctaaagcat agacgattag cggtgcgctg
      601 aatactaatg ctctgttgaa tttacccatt aaccttcacc tctcaaaatg ttctgttttt
      661 tataaatcaa ctaaatgctt ctcgataaat tctttaggtg ttacctttct tgtgcgtaac
      721 ctgttatagg atttgaaaga caaaaattta tcgtataatt ctgtattgat ccaaacttct
      781 tgtcctgtga cccgttcata cgccgctgag aaaattgctg tattttttag ttccgacatt
      841 cgacgttgat aagtagatgg agaataatta tattttttta caaaatcttg tttttttagt
      901 tttgtcatcg cctacccccc ctatcggatg tccaatattt ttttcactgt ttcgatatgc
      961 tgttgtgctt tctttccatc acgattgccg tttagaatat ctgataaata agctcctgaa
     1021 ataccgataa gcgcagctag ttctttgaaa gtcattcttc tttttctcat ctccgctcga
     1081 atttttaagt ctaaattctc agacataaaa atagctcctt tctaaacaaa taatttgtaa
     1141 gctaaaaaat tagctaaatc gttgacacct attagctttt aagctattat gaatacatag
     1201 ttaaataaga cttataaaaa gcctctaaaa taacatttct aagtttggcg accgagagaa
     1261 tgttttaaat tagtagagtt ttttgttgct cttatttagc taacaattta gcttacgaat
     1321 taaatatact agcttaaaag ctaattgtca accaaaaata taacttttaa gctatttatt
     1381 ttcttttcag ctttgaaagg ttgataataa tgagtttagt tactaagatt aaagaattag
     1441 cagacgaaaa gcatgtgact atagcagaag tagaaagaca ggtgggcatc tctaatggac
     1501 aaataagaag atgggataaa gcctcgccaa aatctgagaa cttaaagaaa gttgctgatt
     1561 attttggtgt cacaactgat tatttattgg gaaataataa tgttcccaag tgggctacaa
     1621 aagaggaagt ggttgaactt gataaactac tagactcaaa tgttaatatg tcttatggtg
     1681 gggaaacatt gacacccgaa caaatacagc gcgtaaaaga tatcctgata gcgactttct
     1741 gggatattgt gaaagaagac aaagaaaaag gcaaaaagat gtgagcttat ggagatggat
     1801 acgattaatt tagtcgagga gttgaagcgg aaataccagt ccgctaatcc tttttatatt
     1861 tgtgaaaaga tgggcattaa aattcaatac gttcctttta tcgagaatcc caaggggcag
     1921 tttcaagaaa ttagagatcg tgcgataatc tttttaaatg atgaactgcg agactctgag
     1981 gaaagattct acatttgcgc tcacgaatta ggtcacgcta tttttcatcg tggcttatcc
     2041 agctattatg tatcaacaag aacatctaga agcaaatctg aaagcgaagc taattgcttt
     2101 gctgctaatc tcattgtttc tctttataaa gaagataatg atcaatatcc taaaaaaatc
     2161 gaggaattaa agaatcttta cgggcttcca gaaagcgctt atcgttttct tatataaaaa
     2221 agcccgtgtt agcacacata ttacaacgag aaagaggaat tataaaatga aaaaagttag
     2281 cgttatgttg ttgttaagta ctgctctgct actttcagct tgttcaaata ataaaaaagc
     2341 tgaatcaaca gataccactt ctaaccaaga aacaaaaata agtaaaacaa aagaaacaac
     2401 tgaaaccagt tcatctacta gcaaatctac atctaaaaca gattctagtt caacagttac
     2461 aagctccaac caagctacgg cggaacctag cccaacagtt ataagctcca gtcagagtac
     2521 aatccaaacc gcacctcaag aagaaacata tgaacagatg aaacaacgca ctttacagtc
     2581 aactccagct gatcgtgcaa attggtccaa caaagagtgg gaagctttcg gcgtggccct
     2641 ttatgaaaat ggattgacta cagatgatgc tggcaatatt atcagtcaag atcagaaaga
     2701 acaacaagca gcatctcaac agaatccaga agaccaacaa acaagcgctc agcaagacgc
     2761 tgacacttta tcacttactg attttgttaa caaatacggg atgtcgcctg ttgcatggaa
     2821 agtacagaat ggaatgtctg aagaagaagc attgcgtaca acacagcaaa agacttccgg
     2881 tgaagttcaa ttaggatttt ctaaatacgg aattcaataa tacattttta tgccctacta
     2941 ttttgcctat aatctctaaa aaagttataa agaaaaaagc ccgtgctgca acacggactc
     3001 atacctcatt tctgagatca caaatatatt ataacaagaa gcgagggata tttaaatggc
     3061 aaaaaaagtt atgggtcaag atgggaaaat gtataaagtt aagaaaccgt tttataaacg
     3121 ggtatggttt tggttgttag cggttgttgt ggtgtttatt gctataggtt cgcaaggagg
     3181 cagtgatgat gctaaaaata ccgtcgctga aacaactaaa gaaagcgtga cagaagtgtc
     3241 ttctgcagaa tcggtagcag aatctacagt cgttgaagaa gaaactgaaa ctactgaaac
     3301 tactatagaa gaagttactc aagaagaaag tgttcctcgt gaatacagaa atgcattgag
     3361 caaagctgaa agctatctag gttgggctgg tatgtctgaa caaggtttgc gtgaacaact
     3421 agagtttgaa gaatatccaa gtgatgcaat cgattatgcg ctggctaatg ttgatgtcga
     3481 ttacaacgaa caagctttgg ctaaagcgga aagttacgat gattgggcat caatgtcaga
     3541 ttcgcaattg tacgatcaac ttatatttga aggttttaca aatgagcaag cacaatacgc
     3601 tttagataac ctaccacaat aactaacaaa aacacgcccc tccctcgcca aagtttgtgg
     3661 acgtgatgaa aaataaacct gctataatcg gcttacttat ctattcctat tatagcaaca
     3721 aataggagat gaaaacaatg tggatcgaaa ctaaaactga taaaaacgga aaaaaagtat
     3781 ataaatataa tgagcgatat attgatccaa aaactagaaa aagaaaaaaa gtgagcatta
     3841 cttataaaaa taaatctcga gaaactcaga aagtggcatt gctagagtta aataaaaaaa
     3901 ttgatataaa attaaacgaa aaaacacttc ataagcctga tctaacattt catgagcttg
     3961 ttgaagaatg gttagttata ta
//
LOCUS       DAZKNM010000196         3976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000196 DAZKNM010000000
VERSION     DAZKNM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.202"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..2721
                     /locus_tag="ACG8M9_002547"
     CDS             52..2721
                     /locus_tag="ACG8M9_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0379253.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(3176..3922)
                     /gene="truA"
                     /locus_tag="ACG8M9_002548"
     CDS             complement(3176..3922)
                     /gene="truA"
                     /locus_tag="ACG8M9_002548"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0379254.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         1124 a          793 c          757 g         1302 t
ORIGIN      
        1 aggatatgat aaactgaata caacttcata gaaatttggt ggaaaataaa tatgaaaaaa
       61 aatttaaagt cattctttag agaaaacgga cgctacgcag cagctagttt cctgattccg
      121 tttttgattt tagcacttgt ttatctaagt atcggaatct atcctggaag cagcagaagt
      181 attttggcaa gtgatgcctt ttcacagttt tctaattttc atgccagctt ccgaaatgtt
      241 cttcttggaa agcaaagtat tttttataca tggaatgctt ctctaggatt gaattattta
      301 tctttaattt cttactatct aggcgggatc ttcacgccac tggtgttctt ttttccaaac
      361 cagatgatgc cagatgcact gtatgttctg actttgctta aggtcggctg tgccggattg
      421 gctttttggt ttcttgcacg tacgttcaag attcctcgct gggggcatgt tgcacttagc
      481 gtcagttacg cattgatctc atttatcact gcacattcgg agatcatcat gtggctggat
      541 gcattttttt atcttccatt agtgattttg gggattcatc gtgttatgga tcttaaacgg
      601 ccgaaacttt tatttatcag ttatttactc ttgtttttat ccagttttta tatgggtttt
      661 atgattggcg tattttcttt cttatatttt atcgcccgtc tattgacaaa ctggtcagaa
      721 tataaaaaaa cgattgttcc ttatggtatc acttctttac tggcaggcgg cgcttcaatg
      781 atcattgtat tgccagcagt gctggatctt cggtcaaatg gcgaggcact gagtcagatc
      841 actacgttga aaacagaagc gacttcttat ctggatctaa tcatgaaaaa tatgattggt
      901 gtttatgaca cgacaaaata cggatcgatt ccgttcgttt acgcaggtct gctaccatta
      961 gtcctctgtc tgttttattt tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac
     1021 ggcagtcttt tcgccatact tatcgcaagt ttttatcttg ttccattgaa tttgttctgg
     1081 catggtatgc atgcaccgaa tatgttttta ttccgttatg cctatctgtt ctctttctta
     1141 gtgctcctgc ttgcaggaaa aggatgggaa aagatagaac aagacgatcg tgggctgttg
     1201 ttaggaatca ttttggtcgt agctgctctc tttgcggtgg catggggcat caaacctgaa
     1261 ggaagctaca cctatgtgac gatgacttcc tttgtattga cagtggtttt cttagggatt
     1321 tatgcttttg ctgtgggatt ttatcagcat aaaaaacttt cattcaatca tctgagtata
     1381 ttgttattat tgatcatgtc tgctgaagca ctggtcaata cgaacagtat gatccatggc
     1441 attcttgatg attggaatta tgcttcccgc agtttatata ctgatcctta tcctgacttg
     1501 aaagctcttg taaatgaaac aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg
     1561 gatccagtat ctgcaaacga ttcgatcaac tatggttaca gtggtatcag tttgttttct
     1621 tctataagaa atcgtaattc ttcctcttac ctggataaat taggtttccg ttcaagaggg
     1681 acaaatttga atatccgtta taacaataat actttgctga tggatgggtt tacaggaatc
     1741 aaatataaca tttctaaaaa cgaaagcacg ttcagtaaat atggcttccg ccaagctggt
     1801 caaagcggcg actatacact gtatcagaat gtcaatgcat tgccactagc atttactgca
     1861 ccgttgtccg ttaatgatgt tgagcagcca gtgacagata atttgaccag tcagacaaac
     1921 ttgatcaata cattgtctgg tctaaatgaa cagtatttta cattctatac acctactttg
     1981 aagaatcagt tgaatacgac gatcacaaag acaacgactg gcgtcactta ctcagaaaca
     2041 gcagataacc agccaaaaga gctcacttgg gaagtccatg tgcctgcaaa tacacaagct
     2101 tacttaagtt tgttcccaac caattttgcg caattagaaa gttctagtgc gacgatcact
     2161 gtcaatggta catcgcgaaa aacgcaaatc aacatttctg gtcaatacta tgatcttggc
     2221 tattatcctc aagagacaac agtgacattt actgcaagtt tttatgggac gaaagaaata
     2281 agtttcatgg agcctaaagt cgttggtcta gatgttgtag cctatcaagc cgcaatgaat
     2341 cagatcaaag aaaaaggtgt cgaaatggaa gccactggca gaaaagcttc tgggactgta
     2401 aataccgctg aagattccat gttggtgaca actattcctt atgacaaagg atggaaagct
     2461 aaaattgatg ggaaaccagt caaggtcagc aatttcaaag acgcattctt gatgataaaa
     2521 gttccagcag gtaaacatac tgtcactttc agttacttgc ctgaaggatt tacgataggt
     2581 gcaatccttt ttgtcctatg tattgcattg tttattcttt atgtctggta ccttgaccgc
     2641 aatcgcccct tttatggcac gattgatcca gcaatagaag aacaaccacg aaacacacac
     2701 agaagaagac gtagaaaata aagaaacgat ggaacctgtt cagactgtcc gcaccaaggg
     2761 caatcaaaac aacgttccat cgttttttgg ttacagaaaa aataggtaac aaaaagatga
     2821 ttcacttatc aacgcctcgt ccacaagtca cgatattcat caaagatgag aagctgtttt
     2881 ttgaaattgc tctttgtgac tcagagttaa atgacaggct cacgacctct ttcttaaacg
     2941 gtgttcaatc agctgacctt cggtattagc tcaacaaacg gcaaaatatg aggagctatt
     3001 ttcccgtttg tttctctaat actcaggtca aaacgctgat ttcacgacct cttccttaaa
     3061 cggtgttcca gaagtagctt cttcgaaaat aagccgaaat tttgcaaaat tttgagaagt
     3121 aattttcgta aataccttct tatttcttga agctgaccac ttctatcata acctcttatt
     3181 catatttcac ttcatacaaa taaagtcctt cagggtgtgc tgtaggtcct gctgcattac
     3241 gatctttttt cgcaataata tctgggatac tatcttcagg caatcggcca ttgccaattt
     3301 tcaaaagcgt tcctactaag atccgtatca tcttatacaa gaagccattt cccctaaatg
     3361 taaacaacaa ttcgtctttt gtttcattta cctccatctt agcctcgtag atcgtacgta
     3421 ttttatcctc tatcgagctt cctgatgcac aaaaagaggt gaaatcgtga gtccctaata
     3481 aatcaggaag cgcccgctga attttttcca aatcaagtgg gtaaggaaaa taacttgcat
     3541 aatggcgtcg aaaaggactg cgtggtttcc ctatatccac tcgaaattga tacgtctttt
     3601 ctttgaccaa atatcttgcg tggaagtctt ctgaaacaat ttctacttgt cttacagcaa
     3661 tatcctctgg tgactgggta tctagcgcga aacgcattcg ttctaacgga cgctcctctg
     3721 gataatcaaa atggattact tgcccgatcg catgtactcc ggcatctgtc cttcccgaac
     3781 caaagaccgt gatttctttt ccatttgcca ttttttgtaa tgttttttct acttcttctt
     3841 gaacagtacg cccatttggc tgtttttgga atccattaaa atttgttccg tcgtacgcaa
     3901 tgattgcttt gtaacgtacc atgtttttct ctcctttgtt cctaaaaggt tatctggaat
     3961 aatgattttt ctataa
//
LOCUS       DAZKNM010000197         3871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000197 DAZKNM010000000
VERSION     DAZKNM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.203"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            408..1901
                     /locus_tag="ACG8M9_002549"
     CDS             408..1901
                     /locus_tag="ACG8M9_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0379255.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2036..2332
                     /locus_tag="ACG8M9_002550"
     CDS             2036..2332
                     /locus_tag="ACG8M9_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0379256.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2433..3284
                     /locus_tag="ACG8M9_002551"
     CDS             2433..3284
                     /locus_tag="ACG8M9_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379257.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3305..3517
                     /locus_tag="ACG8M9_002552"
     CDS             3305..3517
                     /locus_tag="ACG8M9_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379258.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1372 a          567 c          788 g         1144 t
ORIGIN      
        1 gagtgaaaag aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt
       61 agcaaaagca gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa
      121 ttgattttag cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg
      181 tctcaagaat atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt
      241 ccgattgcct ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag
      301 tgtacttaaa ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta
      361 aagctaaact gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa
      421 tttattcagc tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga
      481 aacctgcagg tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc
      541 aattaatgaa tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg
      601 aaaagaaact aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt
      661 atcgaacagt ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag
      721 ttgatatcga ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag
      781 tgggctacat gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac
      841 taaagacacc aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa
      901 aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat
      961 gtaaccactt cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga
     1021 actatcagta ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa
     1081 aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc
     1141 aagtagatga accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag
     1201 gacgtcttgg tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt
     1261 atgcattgga tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc
     1321 tatctgaagc agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag
     1381 caagcagaga attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata
     1441 atgatctgtt cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa
     1501 aatatagtca taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc
     1561 ttgctagtga gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc
     1621 ccaagcgaac acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca
     1681 aggtaacacg aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga
     1741 gggttattca actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat
     1801 ttggactcaa cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc
     1861 ctcttaaaga agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt
     1921 gccctataca atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg
     1981 taggtggtat acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc
     2041 gttaaaaaat tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt
     2101 atctgtggaa gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt
     2161 taccagggaa aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc
     2221 ttctggggag actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat
     2281 tgagtataaa atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt
     2341 atttacttaa aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac
     2401 gttaaaggaa agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca
     2461 cctggcttcc tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc
     2521 cattaggaaa actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga
     2581 ttggagtatt tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag
     2641 acatttttac agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat
     2701 tagtcatgca atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg
     2761 atttaattgt tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa
     2821 gaacaacaaa aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca
     2881 tgtatttgtt tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta
     2941 ttaaacgttc aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg
     3001 agtacaatgt tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga
     3061 gtatccatga atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga
     3121 aagcaataga tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat
     3181 atggcatcgg aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt
     3241 tttggaacag tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt
     3301 gaaaatgatt cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa
     3361 gaaggagcag ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg
     3421 tacgatgaac attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt
     3481 gttgattcga aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt
     3541 gatgaatggg aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt
     3601 gtaaagagca taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc
     3661 ctattacgtt tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag
     3721 gatagtgaag gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg
     3781 ctatcctttt taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa
     3841 aatcgtaaga gaaattgatc gaagatgtga c
//
LOCUS       DAZKNM010000198         3862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000198 DAZKNM010000000
VERSION     DAZKNM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.204"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..469)
                     /locus_tag="ACG8M9_002553"
     CDS             complement(248..469)
                     /locus_tag="ACG8M9_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379259.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="ACG8M9_002554"
     CDS             complement(567..812)
                     /locus_tag="ACG8M9_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379260.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="ACG8M9_002555"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="ACG8M9_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="ACG8M9_002556"
     CDS             complement(2036..3037)
                     /locus_tag="ACG8M9_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0379261.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="ACG8M9_002557"
     CDS             complement(3196..3462)
                     /locus_tag="ACG8M9_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379262.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="ACG8M9_002558"
     CDS             complement(3452..3808)
                     /locus_tag="ACG8M9_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0379263.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1220 a          698 c          512 g         1432 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cg
//
LOCUS       DAZKNM010000199         3830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000199 DAZKNM010000000
VERSION     DAZKNM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.205"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..1024
                     /locus_tag="ACG8M9_002559"
     CDS             89..1024
                     /locus_tag="ACG8M9_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0379264.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1109..2119)
                     /gene="gap"
                     /locus_tag="ACG8M9_002560"
     CDS             complement(1109..2119)
                     /gene="gap"
                     /locus_tag="ACG8M9_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0379265.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            2407..3720
                     /gene="obgE"
                     /locus_tag="ACG8M9_002561"
     CDS             2407..3720
                     /gene="obgE"
                     /locus_tag="ACG8M9_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0379266.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         1207 a          678 c          872 g         1073 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacattggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt ttaaaagtag gggatgaaaa gaaagaagcc tttgactgga tccagtcaaa
     1081 ggcttctttt atcgattgat ctatcaaatt acaagttagc gaatttttct agtaagcgaa
     1141 tcatttggca agtaaatcca tactcattgt cataccatgc aacagtttta acaagttgga
     1201 aatcaccagc agttgttact tctgtttgcg ttgggtcaaa gatcgaacct tgagttgttc
     1261 caatgacatc gctagaaacg atttgacggt catcataccc gaatgaagga ttgtcaactg
     1321 tgtgtttttt gattgcttcg tttacttgat cagcagttac ttttgttttc aatactgaaa
     1381 ccaattcagt taatgaacca tcaacgactg gaacacgttg tgcgtgtcct tgtaattttc
     1441 cttgtagctc agggataact aagccgattg ctttcgcagc accagtagaa tgtgggatag
     1501 tattgtcagc agctgaacga gcagcacgca aattaccgcc ttttacaggt ccatctaaga
     1561 gcatttgtgt ggaagtgtag gcatgaactg ttgtcattgt accaacttcg atcccaaatt
     1621 cattatttaa gaaataagcc ataggtgcta aacagtttgt tgtacatgaa cctgctgaga
     1681 tgattttgtc gttttcatct aatgtatcat cattgacgtt atagacgatg gttttcatag
     1741 caccagctgg ggcagaaata actactcgtt tgacaccagc atctaaatgc gcttttgctt
     1801 tttcttcaga tgtataaaaa ccagtacatt ctagtacgat atctacaccg ttttcttttg
     1861 cccaagggat tttgcttgcg tcgggttctg cataaacacg agttgcttcg ccatccacta
     1921 cgattgagtc ttctgttgca gtaactgtgc caggataagt accatgagta gagtcaaatt
     1981 gcaataattg tgctaacata gttggacttg ttaagtcgtt gattgctact acttcaatat
     2041 catctgagac ttctttgatg cggcggaaag ccagacggcc aatacgccca aatccgttaa
     2101 tacctacttt tactgtcata tcaaaagttc ctcctttatt aggggtgctg tgaaacggca
     2161 tcctcaatta atacaattaa tacactgtta ttctgctacg caaagaatat tttgtcaaaa
     2221 aattatactt ggtagcagaa aaaaggttgg aaaccttacc aaatgataaa aaaagaagaa
     2281 ggtaaacaat ctttttgctg aaatgataga caaaatcaga tttcaccgat aaaataagta
     2341 agacttttgt ttattgtgga aattcaggca gtcaaataaa taaatgaata ataggaggac
     2401 ttaattatgt ccatgttttt agatcaagta acgatcgatg tgaaagctgg caagggcgga
     2461 gacggaatgg ttgcatttcg tcgcgaaaaa tacgttcctg acggcggacc ggctggcgga
     2521 gacggtggtc gtggcggcga tgtcatcttg atcgtcgatg aagggttacg tacattgatg
     2581 gattttcggt tcaatcgcca tttcaaagcg cagccaggtg aaaatgggat gagtaaaggg
     2641 atgcacggtc gtggttcaga agatacctat gttaaagtac cacaaggaac cactgtccga
     2701 gatgcggaga ctggtgctct tttaggagat ttaattgaaa atggtcaaac actagttgtg
     2761 gcaaaaggtg gacgcggtgg acgagggaat atccgttttg cctctcctcg gaatcctgca
     2821 ccggaaattg ctgaaaacgg tgaaccagga caagagcgaa aaatcgaact agaattgaaa
     2881 gtattggcag acgttggact tgtaggtttt ccatctgtcg ggaaatcgac tttactatcc
     2941 gttatttcat ctgctcgtcc aaaaatcggc gcttatcatt tcacgacgct cgtacctaat
     3001 ctaggaatgg ttactacaag tgatggcaga agttttgccg cagcagactt gccaggcttg
     3061 attgaaggag cttcgcaagg tgttgggctg ggtacacaat tccttcgtca tatcgaaaga
     3121 acacgagtaa ttttacatgt gatcgatatg agtgggatgg aaggacgaga tccttatgaa
     3181 gactatcttg ccatcaacaa agaattatct acttataact tacgcttgct agagcgtccg
     3241 caaatcatcg tggcaaacaa aatggatatg ccggatgcac cggaaaatct agtgaaattc
     3301 aaagaacagc tgaacaagga aaaagaagat gaatttgctg atgacatccc agtatttcca
     3361 atttctggag tgactcgaca aggattggac gcgttattga atgctacagc agatttgtta
     3421 gaagtaacgc cagaattccc attgtacgaa gaagaactgg aagaagaaac tgtacactat
     3481 ggattcaatc cagaaggtcc tgagttccaa atcgatcgtg attcagatgc aacgtggatc
     3541 ttatctggtg agaagatcga aaaattattc caaatgacga atttcgatca tgatgaaaca
     3601 gtgatgcgtt ttgctcgtca attacgtggt atgggtgtgg atgaagctct tcgggcacgc
     3661 ggtgcgaaag atggcgacct tgtacgtatc ggtgagtttg aatttgaatt tgtcgaatag
     3721 gcaagcaaat aaaaacaaat accaaaaaat atagattcag atttaaggga ggttgtgaca
     3781 gaagtgatca gcttcaagaa cgccgtttat tcggaaaaag gagctgtgat
//
LOCUS       DAZKNM010000200         3825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000200 DAZKNM010000000
VERSION     DAZKNM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.206"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1237)
                     /locus_tag="ACG8M9_002562"
     CDS             complement(<1..1237)
                     /locus_tag="ACG8M9_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379267.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSF"
     gene            complement(1335..1610)
                     /locus_tag="ACG8M9_002563"
     CDS             complement(1335..1610)
                     /locus_tag="ACG8M9_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379268.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            1842..2576
                     /locus_tag="ACG8M9_002564"
     CDS             1842..2576
                     /locus_tag="ACG8M9_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379269.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            2615..3757
                     /locus_tag="ACG8M9_002565"
     CDS             2615..3757
                     /locus_tag="ACG8M9_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0379270.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         1381 a          659 c          565 g         1220 t
ORIGIN      
        1 aaaaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca ctttccttac
       61 tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc atttcctcta
      121 gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta
      181 cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg ttctctaata
      241 gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca actccaataa
      301 aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc ccagttttac
      361 tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt aagtttttac
      421 ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc tcattaaaag
      481 acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct tctttgtcgt
      541 cattttttat caacttaaca aaatcggttt cattgactag ttcatactct ctccctgaat
      601 cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca gtaatatcta
      661 acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca aaaaacattc
      721 tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag tattcgtaaa
      781 cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca atcttgtatt
      841 tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta
      901 aattgataga taaaaccttt tctatgctta agtatctact atcatcttta ctcaaatcta
      961 ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt actctggttt
     1021 ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata aatgattgtg
     1081 atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta tctaaacacc
     1141 tatctcttac atcagttttt tctttttttg taactggctt ctctaataat ttttctttat
     1201 ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc ttataaaatt
     1261 ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta tatgttttag
     1321 tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta
     1381 aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta
     1441 atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga
     1501 gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact
     1561 tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct
     1621 cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat
     1681 aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat
     1741 acacataata cctcgataaa aaagaggtgt caatatcttt gtaaaattta tttacaacac
     1801 ccctttttcg aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa
     1861 taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta
     1921 aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa
     1981 aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa
     2041 ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatata
     2101 ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca tttccagata
     2161 aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat ttagatacat
     2221 ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct aacaagactt
     2281 gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata gaagaattat
     2341 tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact agcagtatag
     2401 aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa gacatgtctt
     2461 atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa caaacgattt
     2521 catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc atctagtggt
     2581 aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa agtacaaaaa
     2641 gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc cacgaactgg
     2701 tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc aaaaagatta
     2761 tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg atatgacatt
     2821 tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag cttcaacaat
     2881 cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta agcttaaact
     2941 atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca gtaaatatga
     3001 aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg ttgcaatgca
     3061 atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa
     3121 aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt ttaaagattt
     3181 tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta tgagaaagag
     3241 tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac tacatgtcaa
     3301 taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga aaacaagagc
     3361 ttctagacga gttatctctt tagatactga gactctttct atcctcaaca actggaaatt
     3421 acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag agcaacatgt
     3481 ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt ggttaagata
     3541 tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac atacacatgc
     3601 tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat tagggcatga
     3661 aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac ctaagaaaac
     3721 aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa aatctggtca
     3781 tttctagtat acaggcaaaa caaaaaagct agaaccctta tatat
//
LOCUS       DAZKNM010000201         3823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000201 DAZKNM010000000
VERSION     DAZKNM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.207"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..1422
                     /gene="tig"
                     /locus_tag="ACG8M9_002566"
     CDS             133..1422
                     /gene="tig"
                     /locus_tag="ACG8M9_002566"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0379271.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            1669..2919
                     /gene="clpX"
                     /locus_tag="ACG8M9_002567"
     CDS             1669..2919
                     /gene="clpX"
                     /locus_tag="ACG8M9_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0379272.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            3002..3589
                     /gene="yihA"
                     /locus_tag="ACG8M9_002568"
     CDS             3002..3589
                     /gene="yihA"
                     /locus_tag="ACG8M9_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0379273.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(3663..3818)
                     /locus_tag="ACG8M9_002569"
     CDS             complement(3663..3818)
                     /locus_tag="ACG8M9_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0379274.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         1353 a          634 c          808 g         1028 t
ORIGIN      
        1 aaaatgttat gaaaaacaag gattctatcc attcaatatg tttacaattt gaaagggtta
       61 gtgtactatt taaaggtatg catgaaacgt ttttgatgta taaaatgaat ttctaaactc
      121 ggagggaatt aaatgactgc aacatttgaa aaaaaaggca cgaatgatgg tgttttaaaa
      181 tttacgattg cgcaagacga aatccaaaaa ggattaacaa ctgcgtttaa taaagtgaaa
      241 aaaagcttga acgtacctgg attccgtaaa gggaaagttt ctcgtcaagt attcaaccgt
      301 atgtacggtg aagaagcttt atatgaagat gcattgaatg ctgtattacc agaagcttat
      361 gaagctgctg tcaaagaagc tggcatcgat cctgtttctc aaccaaaaat cgatgttgaa
      421 agcatggaaa aaggtcaaga ctgggtaatc actgctgaag taacagtgaa accagaagta
      481 aaattaggac aatataaaga tctagttgtt gaaaaacaag accgtgaagt aacagatgct
      541 gacgtggatg cacgtatcaa acgtgaacaa gaagcacaag ctgaattagt tatcaaagaa
      601 gatgctgcag ctgaaaatgg cgatacagta gtaatcgact tcgaaggctt tgtagatggc
      661 gaagcgtttg atggcggaaa aggcgaaaat tactcacttg aattaggttc aggctcattc
      721 attccaggtt ttgaagatca attagttggt cataaatcag gtgaaagtct agatgtaaat
      781 gtaactttcc cagaagatta tcaagcagaa gatcttgctg gaaaagaagc agtcttcaaa
      841 gtgacaatcc atgaagtaaa agcaaaagaa gtgcctgaat tagatgacga attcgcaaaa
      901 gatgttgatg acgaagtatc aacattagac gaattgaaag aaaaatatcg caaagaatta
      961 gtagaaacaa aagaaaaagc agcggacgat gcaaaagatg aagcagcgat ccgtatggct
     1021 gttgaaaacg cagaaatcgt tgaattgcca cacgtgatgg ttcatgatga agtacatcgt
     1081 tcaatggacg aattcttgaa caacatgcaa cgtcaaggta tctctcctga aatgtactat
     1141 caattaactg gttctacaga agaagacttg cataaacaat tcgaaggtga agcagaaatg
     1201 cgcacaagaa caaatcttgt gatcgaagca attgcagctg ctgaaaatct tcaagcaaca
     1261 gaagaagaaa tcaatgcaga aatccaagaa ttatcagaaa catacaacat gccaatcgaa
     1321 caaatcaaac gcgtgttgac agaagatatg ctggaacatg atgtgacaat gaaaaaagca
     1381 atcgaattga tcacaggtac agcgacagaa gcgaaagaat agaaatcaag taataaatca
     1441 tttctgaaga agctacttct agaaaattgt ttattcgaaa taagggactg tgacacgatt
     1501 ttgtcacagt tccttatttt ttgttaagag taaaaaatgc aaaggtatag gtcagttgat
     1561 cctttgttaa acactttttc tttttactaa actatgttat gatagtaaaa gactgtaaag
     1621 tctttgatag tttcttgcat actttttgca agttaagggg tgaagaccat gtatgacaat
     1681 ccaagtagca atgagaccgt acgctgttct ttttgcggga aaacccaaga agaagtgaaa
     1741 aagatcgtag ctggtccagg ggtctacatc tgtaatgaat gtatcgacct atgtaaagaa
     1801 atcattgatg aagaatttta tgaagaagct gtacgcgaat tcactgaagt ccctaaacca
     1861 ctagaaattc ttgaagtatt gaataactac gtgattggac aagatcgcgc aaaacgctcg
     1921 ttagctgttg ccgtatacaa tcactataaa cgtgtcaatc agcaagcaca agaaaacaca
     1981 gatgatgttg aactgcaaaa aagcaatatc tgcttgatcg gacctacagg gtctggtaaa
     2041 acatttttag cacaaacatt agcaaaaaca ttgaatgtac cttttgcaat cgcagatgcg
     2101 acaagtttga cagaagctgg ttatgtgggg gaagatgtag aaaacatttt gcttaaattg
     2161 cttcaatctg ctgattacaa tgtggaactt gcagaaaaag ggatcatcta tattgatgag
     2221 atcgataaaa ttgcgcgtaa aagcgaaaat gtatctatta ctcgtgacgt ttccggcgaa
     2281 ggtgtccaac aagccttgct gaaaattcta gaaggaactg ttgcaagtgt gccacctcag
     2341 ggcggacgta aacatccaca tcaagaattt attcagattg atacgacgaa catattgttc
     2401 atcgtaggtg gtgcatttga tggcatcgaa acgattgtga agaatcgtat gggcgaaaag
     2461 acgattggat ttggtacaca aaaccaaaaa ctttctgaag acgaaagtgt catgcagcat
     2521 atcattcctg aagatttact aaaattcgga ttgatccctg agtttatcgg acgattacct
     2581 gttatggcag ctttagagaa attgacaaca gatgatctgg tacggatctt gactgagccg
     2641 aaaaatgcgc tagtcaaaca atatcaaaaa cttctttctc tagataatac cgaacttgaa
     2701 ttcgaacctg aagcgttgag agccattgcg aaaaaagcaa tcgaacgcaa taccggtgct
     2761 cgaggacttc gttccatcat tgaagaaatc atgatggatg tgatgtttga tatcccttca
     2821 aatgaaaaca tcgaaaaagt gattatcaca aaagaagcag ctgaattgtc tggtgagcca
     2881 acagtaatct ataaagattc agagaaaaaa gcgggttgat gaaaagaggc tgccgtaaaa
     2941 gtcattttgg cttttgcgac agccgcttgc tttattcatc accttgttaa ggagagagac
     3001 catgaaagtc catcaagctg aaattatcat aagtgcagtg gcaccaaaac aatatccaga
     3061 tacacaattg ccagaaatag ctctggcagg acgatcaaat gtagggaaat cttcttttat
     3121 caacacattg attgatcgaa agaatttagc ccgtacttcc ggtaagccag gaaagaccca
     3181 gacattgaat ttttatttga tcgagaacgc attacatttt gtcgatgttc cgggatatgg
     3241 gtatgcaaaa gtatccaaaa ctgagcgagc aaaatgggga aagatgatcg agacatattt
     3301 aacgactaga gaccagctac gtgcggtcgt ttctttagta gatttgcgtc ataagccttc
     3361 tcaagaagat atccagatgt atcaattttt gaagtattat gatatccctg taattgttgt
     3421 tgccactaag gcagataaga tccctcgtgg taaatggaac aagcatgaga gcgaaatcaa
     3481 aaaagcgttg gattttgatc gcaatgacac gtttattctc ttttcttccg ttacaaaaca
     3541 gggaaaagaa gaagcgtggg cagcaatcga acaagcaatt gaatcataaa aagaataaaa
     3601 aagacttacc agtcaaactc tttcataaaa gtttggctgg taagtctttt tttgagctta
     3661 acttactttt gtttcttgtc ttttttcttt tcattttgtg gctgtggttt tagaaatcga
     3721 tcgatctctt cttttggtgc aactgttttt tccggatcga cagcaaatga ttcgaaaagt
     3781 ttatctaaat tatctgtacg ttcaaatttc aaccccattg taa
//
LOCUS       DAZKNM010000202         3808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000202 DAZKNM010000000
VERSION     DAZKNM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.208"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..299
                     /locus_tag="ACG8M9_002570"
     CDS             <1..299
                     /locus_tag="ACG8M9_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379275.1"
                     /translation="ERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLL
                     SLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(425..1102)
                     /gene="nth"
                     /locus_tag="ACG8M9_002571"
     CDS             complement(425..1102)
                     /gene="nth"
                     /locus_tag="ACG8M9_002571"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0379276.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1109..1807)
                     /locus_tag="ACG8M9_002572"
     CDS             complement(1109..1807)
                     /locus_tag="ACG8M9_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0379277.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2075..3259
                     /locus_tag="ACG8M9_002573"
     CDS             2075..3259
                     /locus_tag="ACG8M9_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0379278.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3265..3708)
                     /locus_tag="ACG8M9_002574"
     CDS             complement(3265..3708)
                     /locus_tag="ACG8M9_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0379279.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          988 a          754 c          632 g         1434 t
ORIGIN      
        1 tggaaagaag ctctcatgca atgattctca cttttttatg gttgttgttc accagaatct
       61 atatttttct gatgagcctg aaaccgccca aatccattag tttatgttta gttataaatg
      121 gtattttttt attatcactt atcattttta ttacaccttt agatagatac gtcacttatt
      181 tatataatcc tttggaatac tggacttatt ttataggtat attggaaggc attattttct
      241 acatcgggta ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat
      301 aagttaccat cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg
      361 aaacagcttt tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc
      421 acgtctacag gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc
      481 catcttgaca tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat
      541 gatatctacc aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt
      601 tccgcatcag tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc
      661 gttttgagac acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc
      721 ccaatacgac atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg
      781 acgttggcac ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt
      841 ttgctttatt tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag
      901 cttctgctaa cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca
      961 ctgatacatc tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat
     1021 tgtgcttgag ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca
     1081 ttgttttttg tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt
     1141 tcggcaattc ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc
     1201 ttcgtttttg gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata
     1261 gcaaaatgcg gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg
     1321 ctagcttcaa aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga
     1381 actcgatcga agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga
     1441 tttctttttc ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta
     1501 atagatcata gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg
     1561 tttctatctt gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt
     1621 gtttgattcc catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct
     1681 gtgccatatg caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat
     1741 aatttttaag taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat
     1801 tcaacatgat ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga
     1861 acaggtttct tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat
     1921 ttttgtttgt tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag
     1981 atttcgctgt tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt
     2041 ccaagttaac attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc
     2101 taaagttttt ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc
     2161 tgtacttccg tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat
     2221 cactttgctc atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag
     2281 cttaagtgat cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat
     2341 cggatatttg ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat
     2401 tgaaggattg acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga
     2461 acctaatgaa agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac
     2521 gctgggtcct attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat
     2581 tggagcactc tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct
     2641 tccaataaaa aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt
     2701 acaacaatta ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg
     2761 gttggcagct ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg
     2821 gaaggctggg ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca
     2881 attgttcatt atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg
     2941 gatgttcagt gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc
     3001 gattttgttt ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt
     3061 attcaatggc caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg
     3121 tacccaaagt atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta
     3181 tgctgcagca ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta
     3241 tcactacaat aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc
     3301 gataaaacga aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc
     3361 tcgctgtacc catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg
     3421 cgtcaattga aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc
     3481 tctagctatc agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt
     3541 cgctaatttt gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc
     3601 atttagagta aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc
     3661 attagctaaa tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc
     3721 tttttctttc attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta
     3781 ggaaaaccca atctgaaacc aagaaaaa
//
LOCUS       DAZKNM010000203         3792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000203 DAZKNM010000000
VERSION     DAZKNM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.209"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..980
                     /locus_tag="ACG8M9_002575"
     CDS             <1..980
                     /locus_tag="ACG8M9_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFQ0379280.1"
                     /translation="HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQ
                     NIINLQVKMQTAQVANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSM
                     KAPTLNEISCTIPSHSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPF
                     QILRDRVTYLPQESFFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRY
                     ETLLEEGGNNLSGGQRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKE
                     KTIIFIAHHLPIAKACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            949..2364
                     /locus_tag="ACG8M9_002576"
     CDS             949..2364
                     /locus_tag="ACG8M9_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0379281.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            2397..3431
                     /gene="sppA"
                     /locus_tag="ACG8M9_002577"
     CDS             2397..3431
                     /gene="sppA"
                     /locus_tag="ACG8M9_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0379282.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            3433..>3792
                     /locus_tag="ACG8M9_002578"
     CDS             3433..>3792
                     /locus_tag="ACG8M9_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0379283.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFV"
BASE COUNT         1422 a          531 c          727 g         1112 t
ORIGIN      
        1 aacatttgat tcaattaatt agcagtgcat taattttatg gctaggttct tattttgtta
       61 tggataatag aattagtcta ggtcaactta tcacatacaa tgctttacta gtatttttga
      121 cagatccttt acaaaacatt attaatttac aagtgaaaat gcaaacggct caagttgcaa
      181 ataatcgttt aaatgaaatt ttcgcgattg attcagaaaa atcgaaccag tcttttgaat
      241 atatgctttc tgaacaatta tttcaacaag atatcactgt aaaaaacgtt tcattctcgt
      301 atagcatgaa ggccccaacg ttgaacgaaa tttcatgtac aattccttcg cacagtaaaa
      361 cggctatagt tggagttagt ggttctggaa aatcaactct tgcaaagtta tttgttcgtt
      421 tttatgaagc atcagaagga tcgatccatt atggaaacat taatcactta gacattcctt
      481 ttcagatatt acgcgatcga gtgacatatt tgcctcaaga gtcatttttc tttagtggaa
      541 caatcattga gaatcttata ttcggtttag atagcccacc aagtttcgaa caaataatag
      601 aagtttgtga gatagtccaa ctaaatgaat ttattaatca acagccatta cgttacgaga
      661 cattgttgga agaaggtgga aataatcttt caggagggca acgacaacga ttagccttag
      721 cacgggcttt attaaaaaat ccggacatat taattttaga tgaagcaact agtggattag
      781 ataccttgtt agaacatgca attatgaatt atctcctcaa tttaaaagaa aaaacgatta
      841 tttttattgc gcatcattta ccaatagcaa aagcatgtga tcaaatttta gttctccatg
      901 aagggcattt ggtagaaaaa ggaacgcacg atgaacttcg ttttgccgat ggtatctatc
      961 aaaaattatg ggaaatctag gagagggaaa caatttatgc agaataataa ttggttcgaa
     1021 cattctagta tctatactaa acaacatcat agcttctacc gttgggtgtt ataccctgta
     1081 attctttttt tatgtattat tggattgttt ttaacctttg ctaaaaaaga ggttgttatt
     1141 cggacaacgg ctcaattgaa ccccgaaaaa atagagaagt tacaagtgcc attagaagct
     1201 aaaataatgg aaaacttcct aaaagaaaat caatttgttc ataaggacga tgtgttggtt
     1261 agattggatt gttctttaat agagaatgaa aaggctcaga tagaacaaga aaaccaacgt
     1321 attactcaac aaataaagat ggctcagcta tttattgaaa gtataagtaa aggaaaaaat
     1381 ttgttttcaa cggatgacag ttttggctac agtaatcaat taaagagcat gttgtcagaa
     1441 aaagaatcac tccgctacgc tttgaagcaa agtgaattaa atgatcaaaa gcaattagaa
     1501 gtatacgaaa agacaaaaag acaactagaa aaacaaattg agagttcaga tagtaaatta
     1561 caagaatggc aacaagtaca ggtagcttgg agtaataatc aatcattaaa agatttttca
     1621 aaagaaatga tggcaaacta tgagaattgg caagaacaac taaataatgt ttctgatgat
     1681 caaaaaaatc aagtgaaact gacaatttca gcaagcataa atgaacaaat tgagcaacta
     1741 aaaaaagaag tagaacagta tcagtcagaa aaagctaaat tagttaaacc aactacttct
     1801 gagaatgaca gaattagtca aacggaaaaa ggaaagcaag agctagaaca agcaataaca
     1861 actgtaaaac aaacagttac agagctaaat gaaaaacaag agaagaacca gtcagttatc
     1921 aaaagtttgg acgatcaact ttcaaaagga atactaaaag ctcctgtatc tggaacggtt
     1981 catttaaatg aagaaacaaa gggacaagca gaactagcca aaggaacagt gcttgcagaa
     2041 atttatccga tccacgaaaa atcaaaaatg aaatttacag cacttttacc tgcgaatgaa
     2101 agtacctata ttaaagaagg gatgaaggtt cattttaaat tggatcaaaa aggtggggct
     2161 ccgattacga tcgacggaac tttagatgaa atttcagaaa attctactgc cactgagaaa
     2221 ggtgtttttt atgcagtgaa aggaacgtta aaaacaactc aatcgtttcc gtttcgttat
     2281 ggattaactg gagagttgtc tttaattgta ggagaaaaaa catattggca acaaattgtc
     2341 gatgtgtttc taaataaaaa ataattattt ttactaagaa tataggagga gtttatatga
     2401 ataaaagaag atggattgct gtgggagtag cgtgcggatt attgttacta tccattatcg
     2461 tcgcgctaat tccaggaaaa gataaagagg aagcatcaaa tacaacccta acaggaataa
     2521 ataagatttt ttacggatct aacgaaatca cagaagaaac gttagaagaa ggcgcatcaa
     2581 ataaaaagat tgtcaaacta tccgtcaatg gagttattgc agatacaggt gaaagcaatc
     2641 tttttagtag ggaacaatat aaccatcaga attttttaac acagctaaag aaaattcaag
     2701 aagacaaagc ggttaaggga gtgttattag aagtaaattc acctggggga ggaatatatg
     2761 aaagtgctga gattgctaaa gaaatggcaa aaattaaaaa gctagatatt cctatctata
     2821 ctgcatttaa aaatacagcg gcaagtggag ggtactacat ttctgctggt tcagataaaa
     2881 tatttgctac ggaagaaaca acaactggat caattggtgt cattatttct ggtttaaatt
     2941 attctggctt attagaaaaa cttggcgtaa ctgatgcgac gtataaaagt ggtgcattaa
     3001 aagatatgat gtcacctcaa cacaaacctt cagaggaaga gaataaagtt atacaggaat
     3061 ttgtgatgag tgcttatgat cgttttgtca atgttgtagc aaaaggaaga aatatggata
     3121 ctaatgcagt gaaagaactt gcggatggaa gaatttatga tggtaaccaa gcagtagaaa
     3181 atgggttggt ggatcaaatt ggttactctg aagatgcatt agattcccta aaaaaagaga
     3241 agaaattaac tgatgcgacg gttatagaat ataaaaatga cacaactggc tttgcttctt
     3301 cttggttagg taataagatt gctgaatggc aagggctaaa ggcaactaca tcagatcgtg
     3361 taatttcagt ttttgaaaaa cttggatttt ctgatactcc aaaaccaatg tattactatg
     3421 gagggctata aaatgaataa gaagagtggc attgaaactt tgcctaatta tgtatttgcg
     3481 ggattttggt tacgattttt tgcttttctt attgatttaa tttgtattag catcatcacc
     3541 catgctacta taggtctctt ttacaaaatc atgggactgg aatctgacaa atccattatt
     3601 actttttatg gtatagcgtc attagctatt tatttaacct attttgtact tttgactaag
     3661 ctgaattcag gacaaacaat tgggaaaatg atttttggga taagagtagt gagctttgag
     3721 gaaaaagagc taagttggca aactatcttt attcgtgagt gtatatgtag gtttatatta
     3781 aaaaattttg ta
//
LOCUS       DAZKNM010000204         3741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000204 DAZKNM010000000
VERSION     DAZKNM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.210"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8M9_002579"
     CDS             complement(573..1130)
                     /locus_tag="ACG8M9_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379284.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8M9_002580"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8M9_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379285.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8M9_002581"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8M9_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379286.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG8M9_002582"
     CDS             complement(2887..3165)
                     /locus_tag="ACG8M9_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379287.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG8M9_002583"
     CDS             complement(3199..3450)
                     /locus_tag="ACG8M9_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379288.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1126 a          767 c          506 g         1342 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc t
//
LOCUS       DAZKNM010000205         3735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000205 DAZKNM010000000
VERSION     DAZKNM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.211"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..624
                     /locus_tag="ACG8M9_002584"
     CDS             151..624
                     /locus_tag="ACG8M9_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0379289.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            621..2753
                     /gene="feoB"
                     /locus_tag="ACG8M9_002585"
     CDS             621..2753
                     /gene="feoB"
                     /locus_tag="ACG8M9_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0379290.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2755..2889
                     /locus_tag="ACG8M9_002586"
     CDS             2755..2889
                     /locus_tag="ACG8M9_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0379291.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2983..3627
                     /locus_tag="ACG8M9_002587"
     CDS             2983..3627
                     /locus_tag="ACG8M9_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0379292.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
BASE COUNT         1159 a          674 c          828 g         1074 t
ORIGIN      
        1 tcataatatc acaagtataa ttgacattct caattaccga gagtattcta tgtgagaatg
       61 attctcattt atttgcgtaa cacgtaagaa aattagaaca tataatgaat gattttaata
      121 catgatttat ttcacaagag aggaatcaat atgcagacct tagcgcaggc aaagcagaat
      181 ttcgtttatc aagtcgaacg aatcgaaaca gaagaaaaaa caaaacgcca tttgcaaaat
      241 atcgggatcg ttgccggaag taaaatcgtc gtcgtcaatt tatcaggcaa caatgccatt
      301 ttactggtaa aaagtcatcg agtggcagta agtcgggaaa tactaggtca aatctttgtc
      361 aataaaaaga ctgcttcctc tgaaagctgg acttctcttg atcaattaaa agcaggagag
      421 actgggatcg tcgtagcaat ccacggacaa ggcgcagtga agcgacggtt aatggatatg
      481 ggattgacgc gaaatgtaaa aatagttatc cgaaaactag cccctctggg agatccaatc
      541 gaactgacag tgcgagggta tgaactgacg ttacgaaagt cagaggccga actaatcttg
      601 atacaaaggg aggaaaaaga atgaatatcg cgttagtcgg aaatccaaac agcgggaaaa
      661 ctagcttgtt caatctcttg actggttcga atcaatatgt agggaattgg ccgggcgtaa
      721 cagtagaaaa aaaagaagga tcgttgaaaa aaaatccgaa cattcatatt caggacctac
      781 ccggcatcta ttctttgtct ccttatacac cagaagaagt gattgcaaga gattatttgg
      841 ttcaggaaca gcctgaactg atcatcaaca ttattgatgc taccaattta gaacgaaatc
      901 tttatttaac tacgcagtta ttggatttag acattcctat gatcattggt ctgaatatga
      961 tggatgtcat caaaaaagaa ggaaaaaaaa tcaatctaga aaagctttcc tatggtcttg
     1021 gtgtaccagt tttaccaatc agcgtattga aaaagcaagg aatcgaaaaa ctaatcgaaa
     1081 agattcagga gtctgcaaaa aagcggcctt ctattcctgt tccctgtaca tatgatccac
     1141 gtttagaagc agctttacat gaaattgaag atgtactagg agaacaaagc ggcaagaaac
     1201 gctggtatgc aatgaaatat tttgaacgag atcagaaggt aagagaggat tacgagatag
     1261 cagtttctca gcaaaaagag atcgagcaac tgatccagtt aacagagaaa ctattggacg
     1321 acgatagtga gacgattctg gtcaatgaac gttatgaatt tattacccaa ctatgtacac
     1381 tctgtgttgt gtcaaatgat tctttccaat tatcgatgag tgacaaaatc gatcagattg
     1441 caacgaatcg ttggttagcc ttgccgatct tcgcatttgt gatgtggctg atctattatc
     1501 tggctattca aacagtagga acaatgggga cagattggat caatgacaca ttgtttggta
     1561 catggttacc agaacatgtt ggcagattgt tagctgaatg gcaagtagcg ggatggatgc
     1621 aggagctgat cttgaacggc atcatcgcag gcgtgggagc agtgttagga tttctaccgc
     1681 aattgattat attgttttta tgcttgagct ttctggaaga ctgcggatat atggcgagga
     1741 tcgcttttgt catggatcga cttttcagga aattcggtct ttcaggaaaa tccttcattc
     1801 ctatgctgat tgctacagga tgtggagtgc caggcgtcat ggccagtcgc acgattgaaa
     1861 acgagcaaga tcgtcgtttg acgatcatgg tcacaacctt catgccttgt tctgcaaagt
     1921 tgccgattat tgccttagta gcaggggcct tttttcctca tcaatcttgg gtagctcctt
     1981 ctgcctattt tcttggtatg actgcaatta ttttctctgg aatcagcttg aaaaaaacac
     2041 gtttgtttgc tggagataca gctcctttta tcatggaatt gcccgcatat cattttcctc
     2101 aatggtcagc ggtcctaaga caaacctatg accgcagcaa gtcttttgtt aaaaaagcag
     2161 gaacgattat ctttgtttct agtatcgtga tctggttcag ctcttcttat aatttttatg
     2221 ctcaacctgt ttctgaagac caaagtatct tagccttttt cgggcgaatc ctggcagttg
     2281 tttttcagcc gcttggttgg ggaaactggc aaggaacagt cgcagcgatt acaggattgg
     2341 tcgctaaaga aaatattatt ggcacatttg gtattttgtt tggtcatgcg gaagtatccg
     2401 aaaatggcag agaaatctgg caagtgctac aacacacata tactcccgca gcagcttatt
     2461 cattgttagc cttcaattta ctttgtgcgc cttgttttgc ggcaattggg gctatccatc
     2521 gggaaatgaa tgccaagaaa tggacttgga tcgccatagg ctatcaatgc ggtttagctt
     2581 atttagttag ttttgtgatt tatcagttgg gacacttatt ggagggtgga caggtagcgt
     2641 tagggacgta tcttgctatt ttactgatga taggactcgt ttatgttttg ttccgtcagc
     2701 caaaatccaa gaaccagttt tatactatat tgtcattaga aggagaggaa taggatggcg
     2761 acattcttat taagtgtgtt aatctttgga agtgcaggcg caattgtcta tactcgttta
     2821 aaatccggta agaattgtga tgactgtcaa acagcttgcc cagtaaaaaa ggaacagatc
     2881 aagcgatgat cagtcgttgc aagaggctat tctccccttt tttgcttatt ttgctagagc
     2941 atagtaagat gagggaaagt aaaaaaagaa agaggaaaga aaatgtctgg aaagaaaaaa
     3001 atcgtccgcc cgcgctacca gcagattgct gttgatttag ccgaacgtat cgttgaaaac
     3061 cgatataaag tgggtgagaa gatccatgcc cgctcaacac ttgccagtac gtacaatgct
     3121 tctcctgaga ctacccgcaa ggctatcaat gtgctggtag atcttggaat catggaagta
     3181 cggcatggaa gtggggcatt tgttgcttcg aaggaaaaag caaaagattt cctattgcaa
     3241 tatcaagatg tacaatccat ccaagataca aaagcagaaa tcatggcaag tgtggagaag
     3301 caacaagatg agctgaatca tttttctcaa ttattggatc agctggtcaa tcaaacgaag
     3361 cgaatacata agatgaatcc tttacttccg tttgaattat ctttatcaga aaaagcaatg
     3421 catctggacc gttcaattag cgagttgaat atctggcagg cgactggcgc cacagttatc
     3481 gcaatttctc ataatgaaga actgcttgtt tcacctggcc cctatgctaa atttacggca
     3541 ggggacatcg ttcattttat tggaaatgaa tatacactac agcgtatgac aaactttttt
     3601 tacccggagc atcagcttgc agaatgattg tgtgaaatat taaaaaaata ttaacttggc
     3661 taaacctttt atttacaaag atttagccaa gtttttttca gtttggacaa gcaaatttct
     3721 ttcatttttg acaaa
//
LOCUS       DAZKNM010000206         3693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000206 DAZKNM010000000
VERSION     DAZKNM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.212"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1110
                     /locus_tag="ACG8M9_002588"
     CDS             <1..1110
                     /locus_tag="ACG8M9_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0379293.1"
                     /translation="IVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELV
                     RIKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLE
                     EIEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGF
                     ADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQ
                     LNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREK
                     LSSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNK
                     IYETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            1111..1557
                     /locus_tag="ACG8M9_002589"
     CDS             1111..1557
                     /locus_tag="ACG8M9_002589"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0379294.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            1573..1887
                     /locus_tag="ACG8M9_002590"
     CDS             1573..1887
                     /locus_tag="ACG8M9_002590"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0379295.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            1913..2962
                     /locus_tag="ACG8M9_002591"
     CDS             1913..2962
                     /locus_tag="ACG8M9_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0379296.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            2974..>3693
                     /locus_tag="ACG8M9_002592"
     CDS             2974..>3693
                     /locus_tag="ACG8M9_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0379297.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNK"
BASE COUNT         1293 a          519 c          773 g         1108 t
ORIGIN      
        1 attgtgccaa tagatgaaca agcttatgtt gacattcaca ttacttcgat tgaaaaaaat
       61 gctatgaatc acgacttaga tcaaacgaag ctttcctatt tagatatggg tgttgagcta
      121 caagagttag taagaattaa attaaatgaa ttttatcctg atgaagaatt gatcaataat
      181 ctagtattac atctcaatgc agcagttaaa agactcaaat tgggagtaaa tatctataac
      241 ccttatacgg acaaaatcaa atattctttt aaaaggtcat ttatgataag tgtagattta
      301 ttagaggaaa tcgaagagag attctgtata cattttaatg aagatgaaat cgcttacgta
      361 acactccata ttcaatcact tttggatcgt tacaaacctg acaaaacgaa agtgatatta
      421 gtttgtagta gcggatatgg aacatcaaaa ttattagagc aaagaataaa taacggtttt
      481 gcagatatgg ttgagataga agatgtacta agcatcaatg aactacaaga tagaaaagtc
      541 actgatgaac ttgttataag cacgattccg attgaaaata caaattttcc tgttattgtt
      601 gttagtcctt tgatgttagg ggcagatgtc agaaaagtaa aacagtatct acagttaaat
      661 gaacaaacag aagatgcttt tctaaattta ttagataaac gattttgctt ttttacccac
      721 caaataaagg ataatcaagt gaacgtttta aatataatta ccaaaaaatt ggtaaaagaa
      781 aattacgcga aagaagggat actagaggga gcaatcaaaa gagaaaaact atcttcaaca
      841 gcgatgaatg attttgctat gccgcatatc gaagtagatt ttataaaaaa gccagtaatc
      901 tgtgtctacg ttaatccaga aggaattgat tgggatggga cacttgttca ttctgttttc
      961 ttttttgcac ttaatgaaaa agtaaaacct gaattgaata aaatttatga aacattcaat
     1021 gaaatcgctt cgaatcatga gttattagca aatttaaaaa cgagttcgtc gtttgaaaaa
     1081 ttagtttata tattgaaaga aggcttataa atgatcagtg aaaaaatcaa tataaataat
     1141 atagtgatag atttaaatgc atctgataaa aaaagtgtag tcgaaaaatt atcgaaattg
     1201 ttagagacta atgggtttgt gtctgaccac aaacaatttc aaaaagacgt atttgacaga
     1261 gaaaaccata tgaccacagg aattgggaaa aatatcgcca ttcctcatgg caaaagccta
     1321 gcagtaattg aatcaacagt ggctgtagcc aagttaaagc ggtctgttga atggggcgca
     1381 ttagatgatc aaccagttga tctcgtcttt ttgttagcaa taaaagacac tgataaaggt
     1441 actaatcatt tacgtatttt agcagaatta tctggaagat tgatggatga cgagtttgtt
     1501 aaaagaataa aagattcgag aaataaaaaa gaattggtaa aagctctatc attttaaaag
     1561 gaggaatttc atatgacaaa aatagtagca gtaacagcat gtatcgcagg gattgcccat
     1621 acttatatgg caaaagctaa tttagaaaag tttgcgaaga agaaagggtt agatattaaa
     1681 gtagaaacac agggggctat ggggatagaa aacaaacttt caatttcgga tgtttccgag
     1741 gcagatgttg tcatatttgc agtagataca aatgtttcag aacgaggtcg ctttgacggt
     1801 aaaccgattt taacagtagg gacctcagag gtagtaaaag atggtgaaga gacgattgat
     1861 gaagcaattg cattaattaa caaataaaaa atatatcgtg ggggagttac taatggaatt
     1921 caagaaacaa ggaagagaaa ttctaaatca tttccaaagt ggggtttcct atatgatccc
     1981 gcttgtcgta gcagcagggt tactaacttc tattgcagtc atttttggtg gcacaggtgt
     2041 ttgggaccag acggatacat tctggggagt cctgcgaatg attggtcaga cgggattaca
     2101 gtttatcgta ccgatgattt cagcctatat cgcatattca attgctgacc gtcccggtct
     2161 agcaccagct tttatcactg ggatgatgtg tcaaaatctt ggtatgggat ttattggagg
     2221 aatggttgct ggattagctt gtggctattt agctaatgca ttgaaaaaag taaaagtacc
     2281 gatgcaagtt attagtctaa aatcaattat gctgattcct tttgttacta cattagttgt
     2341 tggaatcata ttatggtatg tactagctac gcctattcaa gcaatgacaa caggactgac
     2401 taattggcta gaaggcatgt caggagcaaa tgctgctttg atgtcggcta ttttaggagg
     2461 aatgatggcg tttgatatgg gaggacctat caataaaatc gcatatgctt ttggggttgc
     2521 ttctttttca agcggaagct ttggacctag tacagccatg ttattaggaa ttgctattcc
     2581 gccatttgga atgtttttag caacaatttt aaacaaaaaa ctttatgatg aatcggaacg
     2641 agataatgca aagacagcta tgattatggg ccttgtgggg attactgaag ggacgatccc
     2701 gtttgccgtg aaagatcctt tacgagtaat accaagcatc gtggtgggca ctgcagtagc
     2761 atgtggtcta aatggatttt ttggtgttac tcaagaaaca atgctttcaa cctttatggc
     2821 aattcctttt gttacgaata tacctttata tttagtatca attgttattg ggggagtggt
     2881 tacagcgtta atggtcaatg cactaaaatc catcaagtat aaaaaagaac aacaagtaaa
     2941 ttcagtagaa gaaggaaagt aggagaatct gatatggtaa atgcacatat aattcaacac
     3001 acgcactggg acagagaatg gtattttaca aatgaagatg ccatcgtatt gtctgatcag
     3061 gtctttactg aagttctaga tgaattggaa aggaacccta aagtaaactt ttgtttagat
     3121 ggacaaactt caatcgtcga tgagtatttg gagatcaatc cgcaaaagct taaggttatc
     3181 caacgactga tgaaaacagg acaactattc gtgggaccgt ggtatgcaca aacagatgcc
     3241 ttattagtgg atgctgaatc aattttaaga aacttaatga ttggaatcag tgatacgatc
     3301 cttaaatatg gagatccaat gatggtaggg tatttgcctg acacgtttgg gttcaatgcg
     3361 aatttgccta cacttttgca tcaagtagaa atcgataatt tcatgtgttg gcgaggatta
     3421 aactttgaaa cgatggttcc atctccttat tttatctgga aaggattggg agataaattt
     3481 gtttatgcca tgaattttcc atttggttat atgactggaa tgatgacctt agacgcaatt
     3541 aaagatattc ctgaatttgc aaaagaaaag ttggatgaag cagttgaaat gcttcatatg
     3601 caaggaaaaa acaaagatat tttagtacct tcgggtgtag atcaaaagtc aatgatttta
     3661 aattttgatc aagtcgtaga gaagttgaat aaa
//
LOCUS       DAZKNM010000207         3665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000207 DAZKNM010000000
VERSION     DAZKNM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.213"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..278
                     /locus_tag="ACG8M9_002593"
     CDS             <1..278
                     /locus_tag="ACG8M9_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0379298.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="ACG8M9_002594"
     CDS             421..642
                     /locus_tag="ACG8M9_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379299.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="ACG8M9_002595"
     CDS             658..1500
                     /locus_tag="ACG8M9_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379300.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="ACG8M9_002596"
     CDS             1563..3365
                     /locus_tag="ACG8M9_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0379301.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3424..>3665)
                     /locus_tag="ACG8M9_002597"
     CDS             complement(3424..>3665)
                     /locus_tag="ACG8M9_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0379302.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1308 a          519 c          746 g         1092 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcccat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata gatacccgtg gtgctagtta
     3421 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
     3481 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
     3541 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtcc caactgtttt
     3601 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
     3661 cggaa
//
LOCUS       DAZKNM010000208         3660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000208 DAZKNM010000000
VERSION     DAZKNM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.214"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..790
                     /locus_tag="ACG8M9_002598"
     CDS             38..790
                     /locus_tag="ACG8M9_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0379303.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            787..1701
                     /gene="pfkB"
                     /locus_tag="ACG8M9_002599"
     CDS             787..1701
                     /gene="pfkB"
                     /locus_tag="ACG8M9_002599"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0379304.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1719..3650
                     /locus_tag="ACG8M9_002600"
     CDS             1719..3650
                     /locus_tag="ACG8M9_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0379305.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1179 a          659 c          836 g          986 t
ORIGIN      
        1 aaaatatgct attatttgta ttgaggtgat cgtgaaaatg cttacagagg aacgcagaca
       61 aaaaatccta gacttgcttg aacagcagaa aatcgtgaaa tcacaagatc ttgtaaacct
      121 tttgaatgct tctgaatcaa cgataagacg tgatttacaa gagctggaga atagtgggtt
      181 tctagaaaga gttcatggcg gagctaagaa agagcaatta ttgaattttg agcagaatat
      241 gagtgaaaaa tcactcaaaa acgttcacga aaaacagcaa atcgccaaat cggcagctga
      301 aaccattaat gacgaagatg tcatttattt agacgctggc tctactacgt tagaaatgat
      361 tccttttctg caaggaaaac agatcacagt agtgacaaat tccgttcaac atgcggcaaa
      421 tttagttgat ttggagcttg ctaccattat tttaggtgga acgataaagc tggcaactaa
      481 tgctgtttta ggatctaatg cgatagaaca actaaaaaat tttcatttta ataaagcatt
      541 tatgggaatg aacggtgccc atcttgaaca aggattcacg actccggatc cagaagaggc
      601 agctgttaaa cgtttagcca tgaaaaattc cgaagagaca tttgtattga ttgatcatac
      661 caaattcaac cagttgtcct tcacctctgt tgcacctttg gaagcagcca cgatcataac
      721 cgagcgttgt ccattagaat tcgttaatga attccgtgaa aagacaacaa ttaaggaggc
      781 aatacaatga tttacacagt aacacttaat ccatcaattg attatatcgt acgcgtagat
      841 gggttaaaac taggcgattt gaaccgaatg aaggaagact tcaagttacc tggaggaaaa
      901 gggatcaatg tttcccgtat tttgaaacgg atcgattctc aatctacagc actagggttt
      961 ttaggagggt tcactggcga atttatttcg aagtggcttg aaaaagaaca aatccagtct
     1021 ttgttcacaa ccgtaaaaga agatacaaga atcaatatca aactaaaatc agacactgaa
     1081 actgagatca atggcttagg cccatctatt tcagatgaag aaatcgatga gttgaagcaa
     1141 gcattaggaa cagtaggaca aggagatatc gtcgtattgt caggaagcgc gccagctagc
     1201 ctgcgaaaag gattttatca agaactgatt gatatcattc gagaaaaagg tgcagatttc
     1261 gtgattgata caacaggtga tgacctaaaa aatgcacttg ttaagaaacc gttgttgatc
     1321 aaaccaaaca atcacgaatt agctgagctt tacaacacaa ccttccaatc tgtagaagaa
     1381 atcattccgt atgggaaaaa attactagct gaaggggcac agaatgtgct gatctcaatg
     1441 gctggagacg gtgctttgct cttcacggaa caaggaactt ataaaacgaa tgtattagtt
     1501 cgcccattga aaaattcagt aggtgcaggg gattcaatga ttgctggctt tattggggct
     1561 tacagccgta cgaaagatcc aatcgaagcc tttaaatggg gagttgcttg cggaagcgca
     1621 acagcttttt ctgatgactt ggcagctcgc gaattgattg atgagttgct gccagaagtt
     1681 gaaatcacaa aaattgatta gtttcaagga ggataatcat gaatatcaag gatttaatgg
     1741 tcaaagaagc gatgatcatg gatttgcaag cgactgataa aaaaggcgca atcgatgaaa
     1801 tggtccaaaa aatgtttgat gccggccgga tttccgatat cgaaacatat aaagaaggaa
     1861 ttctagcccg cgaagcacaa acttctacag gtcttggtga tggaatcgcc atgcctcacg
     1921 cgaaaaacag tgcagttaaa gaagcaacag ttttattcgc aaaaagccaa aaaggagtag
     1981 attatgaagc cttagacgga cagccaactt atctgttttt catgatcgca gctccagaag
     2041 gcgcaaacga cacacatttg caagcattag cagcactatc tcgattgctg attgatgcag
     2101 atttcgtagg aaaattaaaa gaagcaaata caccagaaga agtccaagac ttgttccaaa
     2161 cagctgaaga gcaaaaagaa gcagaagaaa aagcagaaca agaaacacaa gctgcagaag
     2221 cttctgctca tccagaaaga aaattcgttg ttgccgttac agcttgtcca acagggatcg
     2281 ctcacactta catggcagaa gatgctttga agaaaaaagc gaaagaaatg ggtgtggaga
     2341 tcaaagtcga aacgaacggt tcagaaggaa tcaaaaaccg attgactgct gaagacatcg
     2401 cccgtgcaga aggtgtaatc gttgcagcag acaaaaaagt agagatgaat cgctttgacg
     2461 gaaaagaact gatcaatcgt cctgtcagtg atggtatccg taaaccagaa gagttgatca
     2521 atctagccct tagtggtact gcaccagtat tccatggtga tggaaaagaa agcagttcag
     2581 aagaaggaaa tgcggatggt actatcggac aaagaattta caaagatttg atgaacggcg
     2641 tatcacacat gttacctttc gttattggtg gaggaatcgc tattgcgctt tctttcatga
     2701 tcgaccaatt tattggcgtg ccacatgatc aattatcaaa tctagggaat tacaatcaag
     2761 ctgctagctg gttcaaccaa atcggacaag ctgcatttgg tttcatgctg ccggtattag
     2821 caggatttat tgcttcaagt atcggtgacc gccctggttt gatcgttgga tttgctgctg
     2881 gtgcattagc taatacaggc ggtgcaggat tcttaggtgc actgattggt ggattcttag
     2941 ctggttatgt catcgtattt ttacgtaaat tattcaagaa cttaccaaaa tcattggatg
     3001 gaattaaaac gatcctcttt tatccagtgt ttggcttact gattactggt ttcttgatgt
     3061 tgctggtcaa tgtccctatg aaagcaatca atgatgcatt gaacggtttc ttgacaagta
     3121 tgagtggttc gaatgctgct ttacttggtg cattattagc tggaatgatg gcggcagatt
     3181 taggcggtcc aatcaacaaa gccgcttatg tgtttggtac agcaacttta gctacgactg
     3241 ttgcaactgg cggaagtgtc gtgatggctt ctgttatggc tggaggaatg gtacctccat
     3301 tggcaatttt cgttgctaca cgtttgttta agaataaatt cagcaaaaca gatcaagatg
     3361 ctggtttgac gaacatcgtg atgggactat cgtttgtcac ggaaggatcg attccatttg
     3421 cagcagctga cccgattcgt gccattccaa gttttattat tggttctgct ttaactggcg
     3481 gattagtcgg agctttcgga atcaaattac tggctcctca cggcgggatt ttcgttgtat
     3541 tcttactgag tcacccggtc atgtatttgg tgtttattgc tatcggagca gtcgtatctg
     3601 gtgtgattta tggtttattg agaaaagctc ctgcagtagc agaagcatag aaataaaaaa
//
LOCUS       DAZKNM010000209         3645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000209 DAZKNM010000000
VERSION     DAZKNM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.215"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..1992
                     /locus_tag="ACG8M9_002601"
     CDS             226..1992
                     /locus_tag="ACG8M9_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0379306.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            2007..2444
                     /locus_tag="ACG8M9_002602"
     CDS             2007..2444
                     /locus_tag="ACG8M9_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0379307.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2457..3608
                     /locus_tag="ACG8M9_002603"
     CDS             2457..3608
                     /locus_tag="ACG8M9_002603"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0379308.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1232 a          641 c          779 g          993 t
ORIGIN      
        1 atggaaaaca tctaaattaa atgctaacaa aagttattgt ttgcatatta taaaaaaatt
       61 aaaataacag ttttatttaa aattgaagtt ttctgatacc gaaatctttt tattttgctg
      121 acatactcaa acctttggca caaagtttcg tgccaaagta cgactatttc tctttttagt
      181 aagcggatac aattagagta tcaaaaggag agagggagag acaaaatgga acggacgatt
      241 cccaaaaaca acatttcgct aaaagcgagg gtacaaaaat taggcagtac tttatcaagc
      301 atggtcatgc caaatattgg agcactgatt gcctggggtg tattaacggc tctatttatc
      361 ccagatggtt atttaccaaa tgaggctttg gctaccatgg taagcccaat gctaacttat
      421 ctgattcctt tattgatcgg ttatactggc ggaaaagtaa ttgctggtga ccgcggatca
      481 gttgttgggg caattgcaac gatgggtgtg attgtcggaa cagatattcc aatgatgcta
      541 ggcgcgatga tcatgggacc tcttggtgga tatgccatca agaaatttga tcagctattc
      601 caaaaacgga tcaaatccgg ttttgaaatg ctagtcaata atttttcagc aggtcttatc
      661 ggttttggat tagcgttatt aggtttttct gcgattggtc cagttgtcga tgcattgact
      721 caagcaatgg caaaaggtgt agagatcatt ttatctgccc atttgattcc attgacaagt
      781 atttttattg aaccagcaaa aatcttattt ttgaataatg caatcaatca tgggattttg
      841 acaccattgg gaactgaaca agtagcagag gctggaaaat ctattctatt cttgcttgaa
      901 gcaaatcctg gtccaggact tggcgtatta cttgctttta ctttgtttgg caaaggagca
      961 gcaaaatctt cagcaccagg agccatgatc attcactttt taggcgggat ccatgaaatc
     1021 tattttcctt acgttatgat gaaaccgtta ttattcttat cagttatagg cggaggaatg
     1081 tcaggaagtt tcattttcca attgatggat gctggactaa gagcgccagc ttctccaggg
     1141 tcgatcattg cgattctagc gatgacaccg ttcaattctt accttcctgt tattttgggc
     1201 gtgattgttg ccgcagctgt ttcgtttggt atttctgcag taattttgaa agcggatgcg
     1261 aaagatacaa atgaagactt cgaaaaatct gttcaagaga caaaggcagc taagcaagct
     1321 tccaaaggtg cccaaggtat tcctcaagga gctacattct ctggcgtaca aaaaatcatt
     1381 tttgcttgtg atgcaggaat gggatcaagt gcgatgggcg cttcgatctt gagaaaaaaa
     1441 gtacaggaag cagacttgcc tcaaacggtg accaatcaag caatcagcca actgacagat
     1501 gactcgaata cattgatcgt tactcaggtc gaactacaag aaagagcaaa acaaaaagca
     1561 ccgagcgcgc gttttgtctc agtagaaaac tttttgaatt cacctcgata tgatgaaatc
     1621 attcaagaat taagcagcaa gcagaaaaca tcagtttctg aaattgagaa tgagccagca
     1681 gaagagtttc tttcagcaaa catcaaacaa atcaaagaga ttctacttgt ccacgatgat
     1741 cgttccggat cagcaacaat ggggatgacc gtgctcaaag atatattaga caagaaccat
     1801 ttaactatcc cgttaagaaa agtcaatatc caagatttaa aagatgaacc tgattcactg
     1861 atcgtagcca aagaaagttt agcggaaaaa gtacagatgg ctgcacctca atccattcat
     1921 ttatcagtca acagcatggt caatccgcaa aaatacgaat cgattgcaca atatttgaaa
     1981 cagacagctt aagggaggaa aacagcatgc aaagtttgcc attcgacatg atcgagctca
     2041 atcgatcttt taaaaccaaa gaagaagcga tccgctattg cggtcagatg cttgtagaag
     2101 cagggggcgt tgatgaggaa tatatcgatg ccatggtaga tagagacaaa atgttatctg
     2161 tttatatggg gaattttatc gcgattcctc atggaacaga tgaagcaaaa aaatatgtga
     2221 aaaaatccgg catctgtgtc gttcaagtac cagatggtgt agattttggt accgaagaag
     2281 aagaaaagat tgcgactgtt ttgttcggta ttgcaggagt cggagatgag catttgcaac
     2341 ttgtacagca aatcgcactt tattgcagtg atatggacaa tgttgtccag ctagcagacg
     2401 cgctcagcaa agaagaagta acggaaaatc tagcaattgc ttaaggagag agaaaaatga
     2461 gagcagtaca ttttggtgca ggaaatatcg gtcgaggatt tattggagag gttttggcag
     2521 ctaatggatt tgagattact ttcgtagatg tgaatcacac gatcattgat gctttaaatg
     2581 aaagaaaaga atacgaaatc gaattagcag atgaatcaaa agaaaaaatc actgtaaaaa
     2641 atgtcaaagg aatcaacaat caaacaaatc cagaaaaagt aatccaagaa atcgctgaag
     2701 cagatttagt tactaccgcc attggcccga atatcttacc ttttattgct gagttgattg
     2761 caaaaggtat ccaagaaaga gaaaaggaag gaagcacaac atctttagat atcattgcct
     2821 gtgaaaatat gattggcggt tcagcatttc tagaaaaaga agtgtataaa tatcttccgg
     2881 aaacatcatt tactgacaaa tatatcggct ttccaaatgc agcagtcgat cgaatcgtcc
     2941 cattacaaca ccatgaagat cctttattcg tccaagtgga acctttcaaa gaatgggtca
     3001 ttgacgatag ccaaagaaaa aataaaaaaa tccaattaaa aggggtttta tatgtagatg
     3061 atctagaacc atatatcgaa cgcaaactat ttagtgtcaa tactggtcat gcgactgtcg
     3121 cttatacagg tgccttgtta ggttaccaga ccattgatga agcaatgcaa gatgcgttag
     3181 ttgtggcaca attaaaatca gttttgcagg aaacaggaag tttgctgatt gccaaatggg
     3241 ggtttgatgc agagcagcat catgcgtata ttgaaaaaat cattcatcgc ttccaaaaca
     3301 aaaatatctc tgatgcgatc acacgtgtcg ctcggacacc attacgtaag ttaggttatc
     3361 aagaaagatt tacacgtcca gtgagagaac tacaagaaca taacttgact tgcccacatt
     3421 tgacggcaac aatggggatt atttttaatt actatgatcc tgaagatgaa caaagccgtc
     3481 aattgcatga aatgaagatc catgaaaact tggaacaatt gattcaagaa gtcacaggaa
     3541 tcaatgatcc gaaaacaatc gggaatatca agcaaaacgt gaaccgttat gccaagcaag
     3601 tcgcttaaaa taggatacga aaaaggggaa tgtgacgaaa ttttt
//
LOCUS       DAZKNM010000210         3628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000210 DAZKNM010000000
VERSION     DAZKNM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.216"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..1245
                     /locus_tag="ACG8M9_002604"
     CDS             73..1245
                     /locus_tag="ACG8M9_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0379309.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            1826..2020
                     /locus_tag="ACG8M9_002605"
     CDS             1826..2020
                     /locus_tag="ACG8M9_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379310.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            2211..2460
                     /locus_tag="ACG8M9_002606"
                     /pseudo
     CDS             2211..2460
                     /locus_tag="ACG8M9_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2704..3534)
                     /locus_tag="ACG8M9_002607"
     CDS             complement(2704..3534)
                     /locus_tag="ACG8M9_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0379311.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT         1126 a          670 c          735 g         1097 t
ORIGIN      
        1 taaaaggcca tgtaacagcc cttttacggt acaatgtttt taaccacaaa aacataccca
       61 ggaggacgtt acatgaccca agtacatttt acactgaaca acgaagaggt tcaaagtatt
      121 attgaacatt cggtaaaaga tgatgtttct aaaaatattt taaccactgt tttcaaccaa
      181 ttgatggaaa atcaacgaac agaatatatt caagccgatg actatgaacg ttcagaaagt
      241 cgtcagagtc aaagaaatgg ctattatgag cgagacttta cgactcgtgt gggtacactc
      301 gaattaaaag tgcctagaac acgtgatggt gaattttcac cgacggtgtt tgagcgttat
      361 cagcgaaatg aaaaggcact gctcgcttca atgcttgaga tgtatgtttc gggcgtttcg
      421 acacgtaaag tttcaaagat tgttgaagag ctatgtggaa aatctgtatc gaaatctttt
      481 gtttctagcc tgactgagca gttagacccg atggtcaacg aatggcagaa ccgttcactc
      541 tcaggtacga attatcctta tctgatgact gatgttctct acataaaagt ccgtgaggac
      601 catcgagtgc tttctaaaag ctgccatatt gcgatcggga taacagaagg tggcgaccgt
      661 gaaatcattg gcttcatgat tcaaaatgaa gaaagtgatg acacatggtc catcttcttt
      721 gaatacttaa aagaacgcgg cctaaagggg acagaactca tcatttctga tgcccataaa
      781 ggcctagtgt ctgcgattcg taagtcattt accaacgcaa gttggcagag atgccaggtc
      841 cattttttaa gaaacatctt cagttccatt ccaaaaaaga attcaaaacc gtttagagaa
      901 gcagtaaaag cgatctttaa gtttacggat attgaactcg ctcgaacagc taagaatgcc
      961 ttagtcggtg aatatatcga ccagcctaaa tatacaaaag cctgcgaaat attggataat
     1021 ggcttcgaag atgcctttca atacacggtt atcggaaata gtcataatcg gctaaaaagc
     1081 accaaccttc ttgaacgact gaaccaggaa gtccgcagaa gagaaaagat tattcggatt
     1141 tttcccaacc gaacgtctgc caatcgatta attggagctg tccttatgga ccttcatgac
     1201 gaatggctca gttctacaag aaaatatatt aagtttgatc aatgagagac cggtaaaaca
     1261 ttgtatagta ttttacacag gattatggac ttgacttggt tattttataa taaagatatg
     1321 atgactaatt tagctcaact attccagcgt cagctgaaga tattgcaatt cccgattcct
     1381 taatcattta tctggatgag acagaaagtg aagttgcaac agatgtgcat gtagttgagg
     1441 ataaaaatga tagtttaaaa attaatagtt cagatacttt actaagagta agtcctatct
     1501 tttcagctgt tatttagatg caagtagcaa ctaagttagg tagtactcct gcaatggata
     1561 ggtatttgtg tgttcgatat ataagtggtc gaagtatcaa ggttatgtat actggatggg
     1621 agatagaaga gttaaaaatt ggaggtcacc gccacaaatg gaatttagat ttagaggtac
     1681 cttagatttg gtatagttat taaacacaag atggcctggt ttgtagggaa agtcgaagag
     1741 tatttttcca cttctgcgcc cctttaattt ttctagtaat gccgtgaatt tgtgattaat
     1801 tggaaatatg aagaaaggag agtctatgaa aacaacacta gttctattct ataaaaagca
     1861 tccttatttt acacttctga taaatatatt gttggctagt gttatcggga tttctgttga
     1921 atatctaata aataaagatt tcataggttc ttgtttttat acggctttat ttcttggact
     1981 tcttgaagca ttttccattt ataaaaaatc aaaaaaataa aatagattgc cgtaagagat
     2041 ctttttcatt tcattttttt ctgaaaaccc gttgtttctg tcagtgaagc agcgggtttt
     2101 tagatttatt gtctttagac ctaattgagc gcgccgagcg attgaaatac aaaaccactt
     2161 gctatgcggg tggagcaata aagggaaata gtttagcccg tacaaagtag atgtgtaaat
     2221 accacattgt attcacacca aagtatagaa gaaaaataat ttataatcaa tatcgagcca
     2281 atattcagga gatcatcaag agattgtgca aatctaaaat ataaatttgg gtatcgacac
     2341 ttttgggtgg aaggatacta tgtgacaaaa gtaggacaaa atgaagcgac catcaaaaag
     2401 tacattcaag aacaagaaaa acatgatagt gcgttagata agctgagtgt acgagagtaa
     2461 gacgatcctt ttaagggcta aggagtagta cataaacctt ttaagaggtg ggcaaaactg
     2521 gcaaaggcaa tatggcttga acataaaata tgaaagccag cgtctttagg cgctggccgg
     2581 tgatttgagc ttatagctct agagaaaacc acccttttga agggtggtta tgattaatga
     2641 ctttagtcag ttttgctcac aaagcgtgtg gaacaaaaaa cgggtgtttc tacgggtaga
     2701 tatttatttc ttgaatggag gtgtcccata catttttggt gttactggtt ctaattttgg
     2761 tgcagcgcct tcgatcttag gttctttcaa ccattgatat tcgccttcgc catccagtgc
     2821 tttgccacta gcccaagaac cttcactact ttggtctccc tctgagaagt tatagaagtc
     2881 ataagcaatg tcttttcgtt ctttgtcacg cgggaaagta cttgggacta agattgaacc
     2941 ttctttttct tctaattctt tcactgcaca ttcccataga ttttgatgat agctatctct
     3001 tgcaaggaga acagacagca tatcttttac gccacgatca tcgatcattt cgtaaatacg
     3061 tgctacttga agtcttcctt gtgattcggc atttaaatta gctctaaagt ctgctaacaa
     3121 gttgccactc gcaatgatat aggcaccgct ccatggattg cccacgctgt cagcaggacg
     3181 aggtcctaag ccagctacga tcgcgtgctg tggattcatt ccagagataa tggcaccgat
     3241 tgttggatct ttttgcatcg ctgcttcttg atcttcaaca ggcgcaccct ctaataaacg
     3301 agcaaccatt gtcgccaaca tttccacgtg gccgatttct tctgttgcaa tgtccatcag
     3361 catatcttta tatttttctt ctcctcggca gctccagcct tggaacaggt actgcatagc
     3421 tacactcatc tcgccatatt ggccgccgat cagctcctgc agatatttcg cgattaatgg
     3481 attcggacga tctggttttg cttcaaactg caattctttc tggtgtctaa acatcaaaat
     3541 tcctcctaat ctatttttaa tacattctca ttctgctacg aaagattaaa aaattcaaag
     3601 aaagcgctcg cgaattatat aaagtatt
//
LOCUS       DAZKNM010000211         3605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000211 DAZKNM010000000
VERSION     DAZKNM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.217"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1022)
                     /locus_tag="ACG8M9_002608"
     CDS             complement(75..1022)
                     /locus_tag="ACG8M9_002608"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0379312.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1212..1481
                     /gene="rpsN"
                     /locus_tag="ACG8M9_002609"
     CDS             1212..1481
                     /gene="rpsN"
                     /locus_tag="ACG8M9_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0379313.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1657..2019)
                     /locus_tag="ACG8M9_002610"
     CDS             complement(1657..2019)
                     /locus_tag="ACG8M9_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0379314.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2030..3151)
                     /gene="alr"
                     /locus_tag="ACG8M9_002611"
     CDS             complement(2030..3151)
                     /gene="alr"
                     /locus_tag="ACG8M9_002611"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0379315.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3167..3517)
                     /gene="acpS"
                     /locus_tag="ACG8M9_002612"
     CDS             complement(3167..3517)
                     /gene="acpS"
                     /locus_tag="ACG8M9_002612"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0379316.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT          970 a          821 c          658 g         1156 t
ORIGIN      
        1 aaaagctaaa acattttttg tctttgcccc tacaaaaatg tttcagcttt tttcgttttt
       61 tattcctgta tttcttattt ttcgaatgac aggatgattt tcccagcagg aggatgtcct
      121 tcaactagct gatgtgcttg gatgacatgc ttaagtgaag cagacagatt cattgaaata
      181 aaaacatgaa aggctccttt ttggtaggct ttgattatgc cagcaaaggc ttctgcatct
      241 agatattcct ttcttggaac aaacgattca taaaagcctt cctttttttg ccgcaaatct
      301 agatctggga ggtcatttaa tgcaacatac ctgccgcctg gtttcatgat ctggatgccg
      361 gtttctcctt tgatacttcc tttcgtcgca tcgattacaa ggtctgcctg atcagcaaat
      421 tgcagacctg gatttgtttt atcataagca gcaaatgctg aagctcctaa tttttttacc
      481 ttttcctcat ttttactgga tgcactggtt aagattcgga ttcccttttc atggagaagc
      541 tggatcagac ttgacccgac cgcacctgat gctcctagta tcattacagt atctgtcggc
      601 tggatttccg tcaaatggtt gattagatta taagccgtaa ttcctggcgt gaccattccg
      661 gctgcttctt cccaactcat tttatctgga atttttgcta cttttgctga tggcaacacg
      721 acttcttctc cgtaagtccc gccgactgca tggacagcta ctcgatcacc tacatgaaca
      781 gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc catcattacc tggtacataa
      841 gggaatttca atgttcgtat ctctttcatt tctcctaaac gcaaggcaac atcataagga
      901 ttcacactga atgcttttat ttctacacga acatgtccag aaccgacttc tctgggatga
      961 gcatcgattt cttcaaaaac atcttgtgct tctccgtaac cgttaattgc aaatcttcgc
     1021 atggtcacca tctccctttt tctttaagtt tacgtttatg cctttatttt gacaaaaaaa
     1081 atgtcttttc gttttggcgc atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt
     1141 gttaaattat ttatctaaaa gcgtaaagat tacgatttac gctaataaaa aataattgga
     1201 ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga
     1261 ttgaacgcta tgctgcttta cgccaggaat taaaagcgca aaaagattac gaaggcttgc
     1321 gtaaacttcc tttagatgct cgtccagaac gattgaaaaa tcgtgattta atcgatggac
     1381 gccctcgcgg atacatgcgt aaattcggga tgtcccgtat aaaattccgt gaattagcac
     1441 ataaaggttt gattccagga gtaaaaaaag ccagctggta atccaagtct ggctgtaatg
     1501 taaaggagga gggcgcatgg accactcctt ggatcatcaa aaaagatacg ttcgcttctt
     1561 ctagcagaaa tgctggtact agaagcagac gtatcttttt ttacgcacag ctggtccttt
     1621 tttatatgta aaggctgcat ggttacttcg ctatatttat ttagtaacag gagtcagtgc
     1681 ttcgataccc acactaattt tcaatgctcg gtcaacttct gccataatgg gtcgatccaa
     1741 atggcagacc ttttctttca atcttgattt atcgatcgtt ctgatctgct ccaacaggat
     1801 aacggagtct ctttctattc ctgttccagt agctttgatc ccaatgtgag tcggaagctt
     1861 aggtttagcc atttttgccg ttatggccgc aacaatgatc gtcgggctaa aatggttacc
     1921 tagattattt tgaataacca aaactgggcg tgttccgcct tgctccgatc ccacgactgg
     1981 agaaagatca gcaaaataaa tatctcctct ttttaccata ggatcctcct tattcttgat
     2041 attctcttgg tatccgctgt cctaatccgc aagccacttc atagtggatc gtacctaatt
     2101 gatctgctat atcctgcata gtgatttctt tgtctgcatt tttgccgata agcgtcactt
     2161 ttgttccgac aggaaattct tgaggcaatc ggatcatcag ttgatccata catacacgcc
     2221 caatcgtttc acagtagttc ccttctacca atagagaaaa accctgcatc tttcgtggcc
     2281 agccatctgc ataaccgatc ggtatcgttc cgatccactc tgcttcagga gtgatatatg
     2341 tttctccata gccgattcct tcacctgctg ggagtttttt gacttgaatc aattcagaaa
     2401 ccagctcaag tgctggctgc aatggataaa cttccggtag tgcgtgccca gagggattaa
     2461 gtccgtacat tgctactcca taacgaatca tattgccgat tgtttcatca tgccataaag
     2521 ccgttgcact attgctgaca tgtacatatc gaggaagttc gggaagtttt ttcagcactt
     2581 ctataaagcg ttctttttgc agattccaat atgtatcatc tgcctgatct gctgtagaaa
     2641 aatgggtaaa taaaccttcc cattctaata cacgactttc tttgatgaat gcagctgcct
     2701 cttttacttc ttcaggtgta caaaatccga tacgacccat cccagtgtct gcttttagat
     2761 gaatcgaaag aggcgtctct agcttacttt tctctaaaac agcagctgct gcttgcaacc
     2821 attcccgtgt acctgcagtt actgataaat catgagcaac agctacagtc agtgcgtcgt
     2881 aaggaaccat attcaagata aggatgggct gagtaaatcc agcttctctc aactcgactc
     2941 cttcatctaa atttgaaaca caaaagcccg aagctccacc tgcaactgct gcctcagctg
     3001 tttcaatagc tccatgaccg tatccgttcg cttttactac tgcaaacaat tcttttcctt
     3061 gcggaagtct tgcaacttca tttgctacat tttcagttat agctttttta tgtatcactg
     3121 ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc ttctttctag tcttccaaaa
     3181 taatttgtgc aaaagctgtt tcatttgtat gagtaatact gacccataca ttgccttcat
     3241 gaggtgaacg cgtcacaaca ggctgaccat tttcattttt taaaatctcg acatcttgaa
     3301 aggtaacttt tccgatccca gttccccaag ctttagagaa agcctctttg caagcgtaac
     3361 gcccgcctaa aaattctact tggcgatgaa aaggcaagga ctggaatagt ttcatttcat
     3421 cagaggtcaa gattcgtgca atgaattttg gtttttcttc gatcagccga gtgatcctag
     3481 gaagttcgac tgcatcgatc ccaattcctt ttatcatatg cttttctctt ttcaataaat
     3541 ttagaacact ctcattctac caaattataa ccattttgcc acaaataagt ccacaaataa
     3601 caaaa
//
LOCUS       DAZKNM010000212         3605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000212 DAZKNM010000000
VERSION     DAZKNM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.218"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1502)
                     /locus_tag="ACG8M9_002613"
     CDS             complement(183..1502)
                     /locus_tag="ACG8M9_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0379317.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1759..2229
                     /locus_tag="ACG8M9_002614"
     CDS             1759..2229
                     /locus_tag="ACG8M9_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0379318.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2451..3554
                     /locus_tag="ACG8M9_002615"
     CDS             2451..3554
                     /locus_tag="ACG8M9_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0379319.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1149 a          624 c          781 g         1051 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaattaaa agtatgaatt attcaggtag aatctctgaa gaataaatca
     1681 cgtgatcgtg acgataaaat acacttacgt caggtgataa aaaatacata agtagaaaac
     1741 taagaggagg aacagaaaat gaaagaagtt caattgatca gaaaaagcga actatcagaa
     1801 ggtggttgca atgcatgcgg cgtagtagaa gcaacaagct atacattaaa gcttggtgcc
     1861 aacaaagcga taatctctga gttgacagta ggcggtcttg tggacagtct ggcgcttgct
     1921 gaaggtttta ttggagaaga tatttatgag atgttttcag aaatccgtca gttaaaaaaa
     1981 ggggaaaact gcatagaggt tcatcatgag agcccgaatg tgagattcaa acgtggtgat
     2041 aatgagatga tcttcaataa tcatgtgtcc gatcacaccg agctatatgg aatcgtgaat
     2101 caaatactaa ctgagctttt tggattggga ccttatgcgt tcaaggaaga aaacggtaat
     2161 ccaaaattaa acgaagaatg gcaagaaacg attgaaatac aaagaaacaa cccgcactta
     2221 tttcaatagg catccacagt atttcgtaaa agggactttg acacgacttt tgtcaaaatc
     2281 ccatcgtttt ttatctattt gtgttctaaa catttctttc agagtagtaa caactggaat
     2341 gactgaaatg taaacagatt atttgcctgt atcaatacgg aagagtaata taaagtgtgt
     2401 aaacgctatc atattattca ttcaattttt tatagaggga aggcattgaa atggaaaaaa
     2461 cgtatcaagc aggtacaaat gaaggaatag tggattttat caatatggaa gatttagaaa
     2521 tagccgcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
     2581 acttatttac ttatcaagaa aatgaacggg cattcaatca tcagctgatt attcctcatg
     2641 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacaaagaa acgctgactg
     2701 caccaatcat catggcacca gttgctgctc atggcttagc tcatgtgaag gcggagaaag
     2761 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
     2821 gtacattaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
     2881 atatgagcaa agatgatggg atcaatttgg atattcttga agtggccaag cgtaatggag
     2941 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
     3001 gaaatgggtt taccttccca ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
     3061 gccaaacgat ggatgctgtg tacaaatctt ccaaacagaa gcttagtcca aaagatgtag
     3121 aatttattgc tgctcattct gatttgccag tctatgtgaa aggtgtccag tcagaagaag
     3181 atgtctatcg ttcattagaa tctggtgctg gcggaatctg ggtatctaat catggtggcc
     3241 gtcaactaga tggtggtcca gcagcgtttg attcgttgca atatgtggca gaagcagtag
     3301 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg cggacagcat gttttcaaag
     3361 caatcgcatc aggtgcagat cttgttgcta ttggcagacc cgttatttat ggattgtctt
     3421 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
     3481 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
     3541 atcgttttat ttaagaaaca aatgacgaaa aaaataggag tgcggcagat gatgccgtac
     3601 tccta
//
LOCUS       DAZKNM010000213         3541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000213 DAZKNM010000000
VERSION     DAZKNM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.219"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..971)
                     /locus_tag="ACG8M9_002616"
     CDS             complement(267..971)
                     /locus_tag="ACG8M9_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379320.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1158..1403)
                     /locus_tag="ACG8M9_002617"
     CDS             complement(1158..1403)
                     /locus_tag="ACG8M9_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379321.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1420..2271)
                     /locus_tag="ACG8M9_002618"
     CDS             complement(1420..2271)
                     /locus_tag="ACG8M9_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0379322.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2437..3441
                     /locus_tag="ACG8M9_002619"
     CDS             2437..3441
                     /locus_tag="ACG8M9_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0379323.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          604 c          687 g         1152 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat
     1141 aatccgttcc attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa
     1201 taaatgactt catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc
     1261 agttcttcga tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct
     1321 gcatcgatca aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa
     1381 ttcaagcgtt cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg
     1441 gtcttttgct ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt
     1501 taattcaaaa tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg
     1561 aagaggaagg aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc
     1621 atgtgcttca gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg
     1681 actatatgct tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa
     1741 ttcgcctggt gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag
     1801 tgtatctaga tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc
     1861 atacagttct tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa
     1921 agcaacaggg tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt
     1981 caatgattct tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt
     2041 tgttgtcaag aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct
     2101 ttcttcattc ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc
     2161 tttaacagag gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac
     2221 cgggatttcg taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc
     2281 tcctttttta ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt
     2341 gtttctactc ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt
     2401 ttttttccgt aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca
     2461 attctgcata ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat
     2521 ctgacaaaag aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct
     2581 caaacaaaaa ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct
     2641 caagaaaatt ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa
     2701 gaaagtgaag aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg
     2761 atgtacttat ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca
     2821 gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca
     2881 caaaaagcag atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa
     2941 aaaaaatatg agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt
     3001 gttcctgcac cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt
     3061 ggaaaaaaat ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat
     3121 gatacgatag aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag
     3181 gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca
     3241 gaagcacctc ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa
     3301 ttcaagctat caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat
     3361 ttgattgaat ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat
     3421 gaagtattga atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca
     3481 gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt
     3541 g
//
LOCUS       DAZKNM010000214         3417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000214 DAZKNM010000000
VERSION     DAZKNM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.220"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..570
                     /locus_tag="ACG8M9_002620"
     CDS             <1..570
                     /locus_tag="ACG8M9_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0379324.1"
                     /translation="ILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESV
                     SGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFEL
                     IFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLI
                     LISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            580..1236
                     /locus_tag="ACG8M9_002621"
     CDS             580..1236
                     /locus_tag="ACG8M9_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379325.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            1236..2612
                     /locus_tag="ACG8M9_002622"
     CDS             1236..2612
                     /locus_tag="ACG8M9_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0379326.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2742..3155
                     /locus_tag="ACG8M9_002623"
     CDS             2742..3155
                     /locus_tag="ACG8M9_002623"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0379327.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(3180..3417)
                     /locus_tag="ACG8M9_002624"
                     /pseudo
     CDS             complement(3180..3417)
                     /locus_tag="ACG8M9_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1287 a          437 c          600 g         1093 t
ORIGIN      
        1 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
       61 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
      121 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
      181 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
      241 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
      301 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
      361 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
      421 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
      481 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
      541 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
      601 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
      661 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
      721 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
      781 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
      841 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
      901 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
      961 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1021 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     1081 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     1141 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     1201 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     1261 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     1321 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     1381 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     1441 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     1501 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     1561 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     1621 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     1681 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     1741 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     1801 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     1861 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     1921 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     1981 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     2041 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     2101 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     2161 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     2221 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     2281 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     2341 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     2401 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     2461 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     2521 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     2581 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     2641 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     2701 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     2761 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     2821 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     2881 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     2941 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3001 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     3061 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     3121 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     3181 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     3241 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     3301 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     3361 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKNM010000215         3414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000215 DAZKNM010000000
VERSION     DAZKNM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.221"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..259)
                     /locus_tag="ACG8M9_002625"
     CDS             complement(<1..259)
                     /locus_tag="ACG8M9_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0379328.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELD"
     gene            complement(590..823)
                     /locus_tag="ACG8M9_002626"
     CDS             complement(590..823)
                     /locus_tag="ACG8M9_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0379329.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(838..1788)
                     /locus_tag="ACG8M9_002627"
     CDS             complement(838..1788)
                     /locus_tag="ACG8M9_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0379330.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1785..2627)
                     /locus_tag="ACG8M9_002628"
     CDS             complement(1785..2627)
                     /locus_tag="ACG8M9_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0379331.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2629..3348)
                     /locus_tag="ACG8M9_002629"
     CDS             complement(2629..3348)
                     /locus_tag="ACG8M9_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379332.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1121 a          644 c          407 g         1242 t
ORIGIN      
        1 gatctaattc attgaagttc tctctccaac ttttctctat atctatattt tctaagttta
       61 agaggtattt ggtaaactct tcattataca tattaataag cgcattaata tgcggctgaa
      121 taatttcttc aaataacgct tccttagaag taaaatgttt ataaaaagca cccgtagtta
      181 cgcctgcggt caggcacagt tgccttatct tagtttgctg aaatccttct ttttcaaaat
      241 ggcttagtcc actttctatt aacttttggt gagttataat ataatctctg gtttctatgg
      301 gtacttcctc ctaataagat gaattaactt tattatctta cacaggactt gaagtttcaa
      361 tcgttaacgt ttattactga actcattcct tagagctaaa ctaatgagaa agagcagaat
      421 aactgataca aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt
      481 ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc
      541 ataatcactc caatagccat tttcttatcc tccctaaaaa taaagtcttt tacttaaaac
      601 cttttttgta atcgtcaata aattttttcc ctttatcagt taattgaaat cctatatcta
      661 aatagtcctc atatctaagt ttagccagca tctcacttac agatgctgga ctaacactta
      721 gaacttttgc taaatcatta ttttttaatt tcttttttct ttttgataat tcctgaattg
      781 ctagtaaata aattagtata gtagtcgttt taatttgtac catacctacc ctccaaatta
      841 tttcgaaagg ccttcagcaa ttttatccag gttccatttc atcatcgcat agtaactatc
      901 accatcttgt cctttttctg caattgaatc agtaaaaatc gttgaataga taggaatatt
      961 ggtatctttt gatactgttt tcattggtct atcatccaca ctgctttcta cgaataagga
     1021 gggaactttt gttgtgcgta atttttcaac taagtgtttt atttgatctg gtgttccttc
     1081 ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca ttatacgctt tcgagaaata
     1141 tttaaagcat ccttcacttg taacaatcat ttttttatca ttcggaattg aagcaaattt
     1201 agatttagct tctttgtcta gagaatccaa tttttcaata tacttatcta gattttcttt
     1261 atagaatttt ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta
     1321 aataataccg ttttctaaat ttaaccaagc atgaggatct tccttccctt tctcactctg
     1381 accctctaag taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc
     1441 tttgttcgca tttttaacta attttgtaaa ccaagcattt cctccagttt ccaagttaac
     1501 accgttataa aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc
     1561 atattcgtgg ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc
     1621 tatattttcg gtaatatctg ctaaaatcga attagtaact actactttta atttttcgtt
     1681 cgaagccact ttatctttgt ctttactatt tattgaacat gccccgaata taaaaagagc
     1741 aatcaaaatc cccagacttc ccagtactgc tttccatttt ctcatcataa cgatctctcc
     1801 ttctttctta aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc
     1861 gctgttaaaa caatacttga tcctgctgct atgttaaaac tataaccaat aaacaatcca
     1921 agcaatgatg aacttgctcc gactgttgca gaaataaata tcatagtttt caagctattt
     1981 gcatataaat atgctgtagc tgctggtgta attaacattg caacaattaa tatagttccc
     2041 acactttgca tagctgttac tgctactaaa gttaatagaa ccatcaatag ataatgataa
     2101 aaactgacat tcatccctat tgcttttgcc attaatggat caaaagaagt gattaataat
     2161 tctttaaaaa atagaactac tataaccaac actaaaaccg aaacgccaat tgtaatccac
     2221 ttatcaatat cttgtacagc taaaatattt ccaaataaaa tatgaaataa atccgttgag
     2281 ctatttgcaa ctcctataag gataactcct agtgctaaaa aagaactgaa agtaatacca
     2341 attgctgtat ctccttttat tacactattg ctttttataa aagtaataat gatagaagct
     2401 aatagaccaa atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct
     2461 actcctggca aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata
     2521 ataaaacaac ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc
     2581 tgtaaaaagt gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctc
     2641 tccaataaat atagtatctc cataggcttt ttttaaattt tcttcattaa atacagattc
     2701 tacacttccg tgagcaatta gatttcgatt caaaagaata atattatcaa aatacttctt
     2761 taccttgctt aaatcatgat gcacaattag cactgttttt ccctgatgtt tcaatttttt
     2821 taacgtatcc ataattattc gttcactaac taagtctatc cctacaaatg gttcatctaa
     2881 aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc
     2941 tccagataat tctcctattt gatgattaga atattttccc atatcgacct tttccaaagc
     3001 ctttgaaact ctttgccatt ctgcattttt aattctttga aaaactttca ttccggcata
     3061 tgttcccata gatacacatt cttttacttt tattggaaaa gtaaaatcaa tatcagcttt
     3121 ttgctctaca tatgcaatct tcaccagttt tttattgata tcttcattat taatggagac
     3181 ctctccctga tgagggataa tatttaacat tgctttaagc aatgtggatt ttccagcacc
     3241 atttggacca ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa
     3301 tgctaaaaaa tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt
     3361 ctaaatgata atgattatca cgatcatatg gtatacaaaa attatatttt agtc
//
LOCUS       DAZKNM010000216         3409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000216 DAZKNM010000000
VERSION     DAZKNM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.222"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..841
                     /locus_tag="ACG8M9_002630"
     CDS             <1..841
                     /locus_tag="ACG8M9_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0379333.1"
                     /translation="KKYLEVIVQTSDVEDFFYTIKKYRDELFDWAETYDKIKNFHKQN
                     STQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKKADPYSDMMKLNELIQE
                     FIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQA
                     NTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEIDSA
                     AKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTEIT
                     IQF"
     gene            905..>3409
                     /gene="pglX"
                     /locus_tag="ACG8M9_002631"
     CDS             905..>3409
                     /gene="pglX"
                     /locus_tag="ACG8M9_002631"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0379334.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTF"
BASE COUNT         1340 a          435 c          633 g         1001 t
ORIGIN      
        1 aaaaaaatat cttgaagtaa ttgttcaaac gtcagatgtt gaagactttt tttatacaat
       61 caaaaaatat cgcgatgaat tattcgactg ggctgaaaca tatgataaaa ttaaaaattt
      121 ccacaagcaa aatagtactc agaaaaaaat ctgggagaat gcacaagata aaattagatt
      181 aatcgaaaaa agtcggcgtc aaacttctag ccaagaaatt gatcaattga ttcatgagat
      241 gaaaaccatt ttgaaaaagg ctgatcctta ttcagatatg atgaaactaa atgagttgat
      301 tcaagaattt attgaacaat ataatcaact gttagagttg gaaagtcagg aaatattaaa
      361 taaattaacg gaagaaaaac aagcaacatt taaattatta gaaggaaaag attttaagga
      421 tcaatttgta ccaatagttg accaagaatt tcaagaactg aaagcaaaaa ttgaacaagc
      481 gaatactata gatgatcttg ttttcttatc attaaacgta gcagaacagt atttgatctt
      541 taatcaacgt ttcgataatg aagaaaaccg cagacgtcaa gaagaacaag cacgtaaaca
      601 aattgaagca gcaaacttga ttcgtgaaac tgaaaaagat gaaatagact ctgcagcaaa
      661 agctgaagaa atagcgatag taaaacctac tattcaacag aaaacagtac caatgagaac
      721 acttaacgtt catgttaaaa ctattaagtc agcagcagat attgatgagt taacagctga
      781 aattaatcaa aaactgaagg aacaacttca agaaaataca gaaatcacga ttcaattcta
      841 gtgaaaagac aggcataaaa aggtgcctgt cttttcaaag gttacatata gaagggaaag
      901 aaaaatggat aagaaagcga taaagaattt cgcggtcaat gcccgtagaa aattaattga
      961 tgaagtaatg actaaagcaa aacgtttagg aattgatgaa tcaaaaattg aagatgtgaa
     1021 gaaaattgat agcaatttac aagaaatcga aaaatcagga ataagaattc aaggaaaaga
     1081 aatagcgcaa agaaacaaat tgattgcaga actaaaacaa cgtgcttctg tcacttctta
     1141 ttcagaagct tttcaagagt taatcgaaga ggttgcttat acgtggttta accgattgat
     1201 tgccattcgg tttatggagg taaatgatta tttacctgac acgtatcgtg tattatctag
     1261 tgaaataagt gggaaaattg agccagatat tattacagat atttatgatg ccgatatctt
     1321 tgaagaatta tctagtaatg aacagcaaca agtcagaact tggaaaacag ataattcagc
     1381 agaagcaatg gacaagctgt atcaattgat ttttataaaa atctgtaatc aattgaatga
     1441 ttacttacct gagctatttg aagaagtaga agattataca gaacttttgt ttacagcttc
     1501 ttatattaaa aaagatggtg taatcgctga tcttttaagt attccagagg atgatttcta
     1561 tgttcaagct ggtggacagg tagagattat tgggtggttg tatcagtact ataatagtga
     1621 gccacataat caagtggtga atatttataa agggattgtc aaaaaacaag atattccggc
     1681 agcaactcaa ctatttacca cagattgggt cgttcgttat atggttgaca atagtttagg
     1741 aaagtattgg ttagatcatt atcccaatag tgaattaaga aataagttga aatacttaat
     1801 tccagataaa tatgcttttg aacaagcaac atctgtaagt aatttaaaac cagaagaact
     1861 aaaaattatc gataacgcaa tggggtctgg gcatatttta gtttacgcct tcgatgtatt
     1921 gatggaaatt tatcttacgt taggatattc tgagcgtgat gctgcacgtg caatcgttga
     1981 atacaattta tatggtcttg aaatcgataa aagagcgtat caattgtctt attttgcaat
     2041 tatgatgaaa gggcgtcaat acgatcgtct ttttttgaaa aaaagtctat cgccgaattt
     2101 aagagagttt attgattctc ctaaagtgac agacgagtat caagagcgta ttagtgaatt
     2161 gaacaatgat aagtatagtg aacgctggaa tgaatacaaa gagttaattt ttgatcaatt
     2221 tgaaaatggg aaagaacttg gatcaatcat taagttagtc gataaaaata atcaggcaat
     2281 aactaaagaa tacttaggaa aagtaagaga gtatatatca aaattcaacg aatttacgaa
     2341 tatggatatt ctttatggga tgaaggaaat acaagaaaaa tacttgcaca ttcttgacgt
     2401 ggctgaaatg ctagttgata aatatgtagc ggtagtcact aatccgcctt atcttaataa
     2461 aatggataag aaattaaaaa aatatgtaaa tgataactat tctgatgtca aaaaagatct
     2521 attttctgtg tttattaaat tgaatagcca aatgctgatt gataatggat atgcttcctt
     2581 tatgacgcca tttgtttgga tgttcatttc tagctatgaa aagttacgct catttttaat
     2641 taatgaaaga aacatttcta gtttgattca aatggagtat tcagcttttg aagaagcaac
     2701 cgttccaatt tgtacgttta cgataaaaaa ttcaaatgaa caagatggaa gctatataaa
     2761 attatcagat tttaaaggtg gaatggaagt tcaaaaaatt aaaacgttag aagcaattca
     2821 tcagcatggt gaatgcgatt atttttatag tactaatcaa gacaacttca aaaaaatccc
     2881 aggaatgccg attgcttact gggcaagcga taacgtaatc actgattttg ctcaaggaaa
     2941 attaactaac gatgttgcag atgcaaaaca agggttggca accggaaata acaataaatt
     3001 tttgcggtat tggtttgaag tagaatttaa caaaataaag tttgatgcta aaacattaga
     3061 ggacgctaaa aagtctagaa aaaaatggtt tcctacaact aaaggtggtc gttttagaaa
     3121 atggtatgga aacaatgaat acgtggttaa ctgggagaat gatggagatg aaataaaaaa
     3181 ttatgttaat gaaaatggga agttaaaatc tagaccacaa aatcttaatt actattttaa
     3241 aaagggaatt acttggtcga cactttcatc tggacaagta tcttttagat tgcagaatca
     3301 ggcaattttt gaaactaaag gatctgttct gttctcaaag gatataactg actttgaaga
     3361 aatatttggc tatgtaaact ctaaattagt taatcacttt ttaactttt
//
LOCUS       DAZKNM010000217         3403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000217 DAZKNM010000000
VERSION     DAZKNM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.223"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG8M9_002632"
     CDS             <1..135
                     /locus_tag="ACG8M9_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFQ0379335.1"
                     /translation="TDESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV
                     "
     gene            265..726
                     /locus_tag="ACG8M9_002633"
     CDS             265..726
                     /locus_tag="ACG8M9_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0379336.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            760..1395
                     /locus_tag="ACG8M9_002634"
     CDS             760..1395
                     /locus_tag="ACG8M9_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0379337.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            1508..2143
                     /locus_tag="ACG8M9_002635"
     CDS             1508..2143
                     /locus_tag="ACG8M9_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379338.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2136..2270)
                     /locus_tag="ACG8M9_002636"
     CDS             complement(2136..2270)
                     /locus_tag="ACG8M9_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379339.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            2442..>3403
                     /locus_tag="ACG8M9_002637"
     CDS             2442..>3403
                     /locus_tag="ACG8M9_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0379340.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         1373 a          494 c          566 g          970 t
ORIGIN      
        1 actgatgaat ctcagtttaa tctcgaaaaa tttatggagt attattctat tcagaataag
       61 ctcagataca cttgttatgc tgtctgccaa tgctatttta agaaaaaata ttcttacgca
      121 aggcaatatg tatgaataca tcaagaatta ttaaaaaaac cgatttcagc aaaacattaa
      181 aaaagcccgt gctgcaacac gggctctttc ctcatatatg agcttctaca aaaaaaatca
      241 tatcacagaa atgaggaaga aaaaatggaa aaagagattt taataaaatc tccaggaaaa
      301 accttgatta aagtaactga tgattctatt tcaattatta gaaaaggatt tataaattta
      361 gttaatcaag gtattaaagg agaaaaaact atccccttta aaaatatatc agccgtacaa
      421 ttaaaaaaac caggtatgag taatggttat atacaattta ccttgctagg cggaaacgaa
      481 agtcgcggag gtatattagc ggccacgaaa gatgaaaaca ccgttatgtt tacaaaaaaa
      541 tattggaatg aaatggaaaa tttaaaaaaa tatatagaga agcaacaatc tatattagat
      601 aataattcca aagaaacaca aatttcaagt gcagatgaaa ttaaaaagta taaagagttg
      661 ttagatgaag ggattattaa tcaagaagaa ttcgatttta agaaaaaaga attgcttggt
      721 ttataatatt caatatcaaa agagaaagag gatctattaa tggacggtat atttggtttt
      781 actggtattg ttttatttat tataggttta atcatgttaa ttgtaagatt tatcaaaaaa
      841 acaaataaga aaacacctat gattctcctt attatcggga ttatttttac taccgtaggt
      901 ttctcgcttt cctcatcaaa cgaggcaaat gacaataaca aacaggaaag ctcatcatct
      961 gatactcaaa ctactacatc taacagcagc aataaaagcc taactcaatt tgaagaattt
     1021 ttagagacaa ataatcatga ctggggtgct ttcttagata gttattattc tattactcca
     1081 gctactgagc aaaatacagc ttttactaaa tatattagtg gtaaaacata tacttttgaa
     1141 ggaactgtta tcgaatcaat gactactaga atagccatta tagcagacaa agagtacgat
     1201 gataaaagtt ggaatgatat ttctgcaaca cctaaagttt catatgtaat ttttgcgaag
     1261 gacttaaaca atgctgatac ctttacaaag ggcgataaag taacttttac aggagaaatt
     1321 agttcaagag gatcgaacat agaaaaatcc tatgctcaat gggatatgat taatagtaaa
     1381 gtaagtaaaa aataaatgaa ccacgcccca ctaagagtta gattgtgcac aataataaaa
     1441 cctatacaaa agagtgccat caggagtcac aatgcttgta aaggaaatga caaaatagga
     1501 ggtaactatg gataatagca atgaaaaact aaccaaaaaa gaatatcttg aattaatagt
     1561 tgaaggcggt attggagcaa ttccttatat tggtggcgtt gtgcagtcac tttattttgg
     1621 gtctaaaaac gagaagcgtt tcaaacgcat cgagaaattc tacaaaaatt taaacgaatc
     1681 attagaaaac attcgcgatc aaattccaga agatgttttc aacttagaga acaaggacca
     1741 actcattgga atattcgaag ggataaatga cgaggtagaa aaatcaaagg cacaaaataa
     1801 aatagatatg tataaaaatc tttataagaa ttgtttactt cgaattaaca acgcttcatg
     1861 ggataacgag gagtattatc ttcttgtact gaatcaatta acatcgattg agattcaatt
     1921 gttagcatgc ttaatgagtc gtggaaacga aaatttcact ggaaatatca gcatgtctgg
     1981 ctattcacaa gagctaatag atggtagctt aaatagatta tctgattttg gtctgttaga
     2041 aaaaacgatt agctccatag tattaggtga tgtcggcaaa caaaatatgg gctacaaaat
     2101 atctcattta ggaattcaat ttattaactc aactcttact taagttttct ttctaatatg
     2161 acatctagac ctatcagcaa tgcaacaata aaaaaccaga cataccaata atcaacaaat
     2221 aatctaaact ctggaagttc agttattctt ttaaaaatca ctttaatcat aataaacacc
     2281 tctgattgga tgatctaata cacatccaat tataccatta aaacgacaaa tagcacatcc
     2341 ttgcgccggc aagcaaaaag gatgtgcaaa aaataataaa acccaaacaa tgggcttctt
     2401 tatagttcct attgtatcag agaaagagag ctgattcaat tatggcaaga ttagtcaaac
     2461 gtggaaatag ttggcaatac gaaatttcat acaaaaaaga tgacggaaaa tacacgaaga
     2521 taagaaaatc aggatttaag acaaaaggag aagcaaaaga tgccgccaac gaattagaat
     2581 ataacttgaa caaaggcctt aaaggggatc gcaaaaatct attattatca gattactttg
     2641 aggattggat gcaactttat aaagaaggaa cagtatctcc tatcacttat agaaaatacg
     2701 aagatacgtt aatgaacata aagaaatata tgccagcggt attgatttct gatttagata
     2761 gagtgggata tcaacgcttt ttaaataagt atgctaaaga ccatgtaaaa tctaccgtta
     2821 ttaagtttaa taaccatatt agagcgtcgt tgaaagatgc cgtagaagaa ggattaattc
     2881 cgtttgatcc aactagaaaa gcagtaatca aaggaaaaga ttcattgaag ccaaaagaag
     2941 ataaatattt agattatgat caatttaaat ctttaatgaa actcgtagaa gaaaaccttt
     3001 ctgcgcagta ctcttctcct atgctcgtgt tagttgctgg tgccactgga atgcgatttg
     3061 ctggacttct aggattaaca tgggaagata tcgatttcga agatcaaatc atcactatta
     3121 ataaaacatg gaattataaa ttaaatgaat ggggaaaaac aaaaaacgaa acttcaaata
     3181 ggaaaatttc cattgataaa catacgattg atctcttaaa aaagtttaaa atcaatcaaa
     3241 aagaattatt cgagaatttt gaaatcaaaa accctcataa ttttgttttt ttcaacttaa
     3301 aaaatggatt agtttcatca aacgccgtca gcaaatattt gcgcaaaaaa ttaaaagaat
     3361 tagggattga aaagcaattt actttgcatg gactaaggca tac
//
LOCUS       DAZKNM010000218         3390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000218 DAZKNM010000000
VERSION     DAZKNM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.224"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8M9_002638"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8M9_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0379341.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8M9_002639"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8M9_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          950 a          747 c          538 g         1155 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga
//
LOCUS       DAZKNM010000219         3265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000219 DAZKNM010000000
VERSION     DAZKNM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.225"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1693)
                     /locus_tag="ACG8M9_002640"
     CDS             complement(227..1693)
                     /locus_tag="ACG8M9_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0379342.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2004..>3265)
                     /locus_tag="ACG8M9_002641"
     CDS             complement(2004..>3265)
                     /locus_tag="ACG8M9_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0379343.1"
                     /translation="QVEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVV
                     GLAVLLAVLPPLVVSRAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGV
                     LVKGSNYLELLAQTETVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTH
                     PIAQSVLEGYAGPLLPTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEI
                     GTLLYLAIDQSYSGYLVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQ
                     VGVDKIYSELLPEDKVQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSD
                     AAIEAADVVIMNDQPSKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMG
                     DAVFADVGVTVLAVLNAMRSLHVKNKR"
BASE COUNT          999 a          676 c          540 g         1050 t
ORIGIN      
        1 aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat aaatggtgtt
       61 caatcagctg accttcagca ttagctcaaa aaacggcaaa atatgaggag ctatttttgt
      121 ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat tcacggtgtt
      181 ccagaagtag cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
      241 ctgtttctgg ttcagtaatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
      301 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
      361 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
      421 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
      481 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
      541 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
      601 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
      661 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
      721 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
      781 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
      841 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggga
      901 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
      961 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
     1021 tgacataagg ggatattttt tccgcccctc caactgcaaa tactagcatc gaaacggttg
     1081 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
     1141 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
     1201 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
     1261 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
     1321 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccacca gcaatcagaa
     1381 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
     1441 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
     1501 tatccttgaa ggctgatcct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
     1561 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
     1621 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
     1681 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
     1741 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
     1801 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
     1861 gaccttattg aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt
     1921 tataatccta tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga
     1981 cgttcattcg tcctcttcat tgatcaacgt ttatttttta catgcaagct tcgcatggca
     2041 ttcaaaacag ctaatactgt caccccaaca tctgcaaata ctgcatctcc cattgatgca
     2101 aaaccaaaag cacctaagga aagtaccaaa atttttatcc caatagcaaa gacaatgttc
     2161 tgtttgacga ttttcaatgt tttctttgct aaatggattg cttctgcaat cttcgatggt
     2221 tgatcattca tgatgactac atctgctgct tcaattgcag catcgcttcc caatccaccc
     2281 attgcgattc ctacatctgc tcttgctagc accggtgcat cgttgattcc atctccgaca
     2341 aatgcggtct ttttattgtt actttgtaag atttcctcta accgctgcac tttatcttct
     2401 ggaagcaatt cgctatatat cttgtccacg cctacttgct tcccgatatg atcagcaatt
     2461 tttttcgagt cgcccgtcaa catgactgtg tttttcacac cagcctgttt taattgaacc
     2521 aatgcgtcta cggcatcttc tttcaaggta tctgcaataa caagataacc actgtaactt
     2581 tgatcgatcg ctagataaag caatgtaccg atttcttgac ttgaaggaaa agagatttca
     2641 aatctttcca tcaatttgtg attacctact aatacttgtt tgccatcaac ttcagctaag
     2701 atcccttcac ctgcaaattc ctggatatta gctgttggta aaagcggacc tgcgtagcct
     2761 tctaaaacag attgagcaat cggatgtgta gaaaactgtt ctgcacttgc aacatattgc
     2821 ataaatttct ttggatcaat tcctgttgga tcgatcgtct ggatcgaaaa gtcacctttc
     2881 gttaatgtgc cagttttatc gaataccact gtctcagttt gtgcaagcaa ctccaagtaa
     2941 tttgaacctt ttactaatac accgatttta cttgctcctc cgataccgcc aaagaaacta
     3001 agtggcacgg aaatcactaa tgcacatgga catgagatca ctaaaaatgt caatgctcga
     3061 tagatccatt cagaaaacgc cgctctagaa acaacaagtg gaggtaaaac agctaataaa
     3121 acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat gaaattttca
     3181 gctggtgctt ttttactgct ggcattttct actaattcta ataatttgct gatagtagaa
     3241 ttttcataag atttttctac ttgaa
//
LOCUS       DAZKNM010000220         3265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000220 DAZKNM010000000
VERSION     DAZKNM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.226"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..776)
                     /locus_tag="ACG8M9_002642"
     CDS             complement(<1..776)
                     /locus_tag="ACG8M9_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0379344.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIM"
     gene            complement(896..1543)
                     /locus_tag="ACG8M9_002643"
     CDS             complement(896..1543)
                     /locus_tag="ACG8M9_002643"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0379345.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1627..3054)
                     /gene="uxaC"
                     /locus_tag="ACG8M9_002644"
     CDS             complement(1627..3054)
                     /gene="uxaC"
                     /locus_tag="ACG8M9_002644"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0379346.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3128..>3265)
                     /locus_tag="ACG8M9_002645"
     CDS             complement(3128..>3265)
                     /locus_tag="ACG8M9_002645"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0379347.1"
                     /translation="GYIRPDHGRMIWGETGRPGYGLYDRALGAVYLNGIWEALEKGAS
                     K"
BASE COUNT         1042 a          688 c          439 g         1096 t
ORIGIN      
        1 ctcataatta ataattgagc aaggagtaag aaaatagccg caccaattat agaaagtaat
       61 gccctatttt taactaaccc ttttaaagat tttaacaatg aaactttgta ttgagattgg
      121 tcaattctta ctcgttcagt agttagttta taacatatta aataacaaat aagtgcacat
      181 atcgaaaata ttcccgcaac aattgtaaaa gttgatccac cacgaactat ttgagcgcct
      241 gaatcatctc ttgtataaat aaataatggt gttaatacac caatgaccaa acttgctaaa
      301 gtagctccaa catttcggaa tgtcgatagc attgttcttt cttctgtaat agaagtaata
      361 gctgatgcca ttgatccata aggaatattt atagatgtgt aaaaaatact cccccataat
      421 atatacgtaa catacatgta tactacctta acgctcatag aagctgaaat catagaagtt
      481 tgatacatta aaaaacttgc taaagcgact ggaccactca ttcttaaaat ccacggttta
      541 aacttaccct gcttagttgc tttcgatcta tcaacaatac gccccatagt tacatcagta
      601 actgcatcga taaatcttgc tactaaaaat aatgttccaa cggcagctcc gcttatcccc
      661 aatacttttg tataaaagat cattaaaaaa gaactagcaa aaatgaaagt aaaatcgttt
      721 ccaaaatctc caaacatata tcctattttg tctcttatac caaattttct ttccatacaa
      781 ttctctccct tctgttgtta agcgttttca ttctgatgaa agtacgtact atactctgaa
      841 gcgtaaagag tcagtattta ttttttacat ttcagagtat tatttgtttt ttctcctaat
      901 aattctttat ctcataaaac ttatctatat atttactagc tactcgactt acttcttcaa
      961 aatcaccttt ttcaacagaa gccaataaat tcccacctac accaactgca acaactccta
     1021 cttcaaacca atcttctaag ttctctatac ttactccgcc agtaggcatt atatttacgt
     1081 gtggtaatgg agcttgatag gcttttacaa tggatggacc aaaagcagaa cctgggaata
     1141 gtttaatgat atctacgcca gcagtaagtg cctcctttat ctcagtaatt gtcatacaac
     1201 ctggtaaata aggaatttga tataaattac aaatctcagc tgtttgtcga tcaaaagatg
     1261 gactaacgat atattctgcc ccagccataa ttgcgatacg agcagtaaac gcatctaaaa
     1321 ccgtaccggc tccgattatt acatcttttt gatcagcata ttttttagca atttctttga
     1381 ttatttcttc agcattagga actgtaaaag ttagttcaat ccctttaatt ccacccgcaa
     1441 taattgaatt tattgcttta atggcctcat ccttattttt cccacgtaca accgcaatga
     1501 ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc tccttccttt
     1561 atggatacat actttgtcta gctgtactaa cttacaaaga ataaatttaa gcttttattt
     1621 caagtattat tttctacttc cgatttcaaa aaaattaaaa taattatacg cattacgata
     1681 acaaatattc tccactatct gactaagata catttcatca tctggaattc ggcctgcttt
     1741 aacccactcg cttagtaatt gacataatac tcgacgaaaa tactcatggc gaggatagct
     1801 tagaaaactt cgagaatcag tcaacatacc tgtaaaattc gacaacaaac tttgttgtgc
     1861 catcgtagtc aactgttgac gcattccttc agctgtatca ttgaaccacc aagcacatcc
     1921 caattggatt ttttgaatca tacccccttg gaagctttgc attgctgttg ctagtggaag
     1981 ccaatcagtt ggatttaatg aatataaaat tgtttttggt aagcacccac tcttttcagc
     2041 ggtatcgaac aatgctacaa cattttgtgt caaactagct tgatccccca tcgaatcacc
     2101 accgacatct actccaattt gttcctttaa tatacttgaa ttatttctta aagcatttaa
     2161 atgcatttgc atcgtccaac catatcttgt atactcagac atcaaatgta attgaatacc
     2221 tgtaacaaat tgtgcaattt ctgataaatg gaaatccgtt tttcctgcta aagcttttat
     2281 taacagattg tcgagtattt tttcatctgc tatttcataa aaataagtat tcaaaccgtg
     2341 atctgctaat cgacagccaa tttgatgaaa atgatccact cgttggctca atgcttgctt
     2401 caacccatta aagtctttga ttggtatagc cgctgtttga ctaagtttta aaatatagtt
     2461 cgaaaagcct gatgatttga tactccagac cttatctgga cggaaagtag gtaatacctt
     2521 gaaagaatta tcctgtttag aaagctcagt atgataccac aagtcatctg taggatcatc
     2581 cgttgtacag atggctttca catccatcat ctccaacaat tctttcgggc gataggaatc
     2641 cgtttgaatt ttttcgttag cacgtcgcca aatttctggt gcattcttct cagaaattgt
     2701 caaatcaata tcaaagtaac gttgtaattc caaatgagac cattcaaaaa taggattacc
     2761 tggcgccttc tccactgttt ttacaaaagc taaaaatttt tcataatcat cacctggacc
     2821 actgatcaac tcttcttcta taccattagc tcgcatcaat cgccatttat aatgatctcc
     2881 agctccttta tcatttaacc atacctgagt caaattcttg tattgcttat tttctagcaa
     2941 atatgctggg tctaaatgac aatgatagtc gataattggt tggtgttttg caaatttatg
     3001 aaaaagtctt ttagctattt cattctctaa taaaaagttc ttatctaaaa acatatttaa
     3061 acactccttt ccttccgtta gtttttagaa gagaaaattt ttcctctaaa taaactccct
     3121 tatataatta tttactcgcc cccttttcaa gcgcttccca aataccattt aaataaactg
     3181 cacctagagc tcggtcatat aatccataac caggtctacc ggtttctccc caaatcatcc
     3241 gaccatgatc tggacgaata tagcc
//
LOCUS       DAZKNM010000221         3244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000221 DAZKNM010000000
VERSION     DAZKNM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.227"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(394..3099)
                     /locus_tag="ACG8M9_002646"
     CDS             complement(394..3099)
                     /locus_tag="ACG8M9_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0379348.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT          836 a          660 c          600 g         1148 t
ORIGIN      
        1 aattctataa taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat
       61 cttaactttt ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag
      121 catagcgcag ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa
      181 taagcccaac caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg
      241 atgcagagca gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt
      301 attagctcaa aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact
      361 caggtcaaaa cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc
      421 cgcttctttg aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat
      481 tccaaagaca acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata
      541 aattcctcct gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt
      601 gatcatttgg aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat
      661 cgacagtact aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa
      721 gacatagacg atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac
      781 cggattttgg acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg
      841 cgcaatcgcc attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa
      901 attgtttcgg tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac
      961 agcgccgacc cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc
     1021 gattggataa agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt
     1081 tgttgcgtct agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt
     1141 tcggatcgcg tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat
     1201 caagtccgca gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt
     1261 caatgtaccg gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc
     1321 caatcctaat ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt
     1381 gatgccattg acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc
     1441 ttgtccaatt gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac
     1501 agttgctttc gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc
     1561 gccttgtgca gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact
     1621 gatttgacct gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat
     1681 ttgatctagc aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc
     1741 agataggcgg ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat
     1801 tggctgtaat ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt
     1861 atcgattgca ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat
     1921 gtttttcttt tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat
     1981 taacgaacct gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt
     2041 tgtgatgctt ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag
     2101 atcacttgcc tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat
     2161 gatcgtcaag ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc
     2221 aaaatcgccg tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc
     2281 ttgcaaatca aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc
     2341 aacgactttt tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt
     2401 attcagtttt tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta
     2461 gccgtcgatc gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat
     2521 acttactagg ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga
     2581 actggctgtt ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc
     2641 ggtgattttc ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt
     2701 cttgatatca ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat
     2761 acctgcgtaa tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa
     2821 ttgccatcct aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac
     2881 ttctttacct tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc
     2941 agtatttcca tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat
     3001 gatcatgatc gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc
     3061 aagcaaatat aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt
     3121 ttcagctgaa ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa
     3181 gtcgaatttt tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa
     3241 taat
//
LOCUS       DAZKNM010000222         3235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000222 DAZKNM010000000
VERSION     DAZKNM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.228"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..1238
                     /locus_tag="ACG8M9_002647"
     CDS             45..1238
                     /locus_tag="ACG8M9_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0379349.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            1231..2958
                     /locus_tag="ACG8M9_002648"
     CDS             1231..2958
                     /locus_tag="ACG8M9_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0379350.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         1096 a          552 c          726 g          861 t
ORIGIN      
        1 ttttcagaaa gaaaaatgaa tttacataaa aaaggagaat ttgtttggta aaagtagaag
       61 taaaacattt aacaaagata tttggtaaaa agacacaagc cgctttagat atgatgaatg
      121 atcaccagcc gaaaacggag attttaaaga aaaccggtgc aacggttggt gtgtatgacg
      181 tgaattttga cgtaaaagaa ggagaaatct tcgtgatcat ggggctttcc gggagtggga
      241 aatccacatt gatccgtcta ttgaatcgtc tgattgagcc aacttcgggc agtatctata
      301 ttgatggaga agatgtatcg aagttaagca aagaagaatt gcgagaagtc cgtcgtcata
      361 aaatcaatat ggttttccaa aacttcggtc tgtttccaca tcgaacgatc ttggaaaata
      421 cagaatacgg gttagaagtc cgtggtgtac ctaaagaaga acgacaagaa aaagcagaaa
      481 aagcattaga aaattctagt ttgctttcat ttaaagacca atatcctagt cagttatctg
      541 gtgggatgca acaacgggtt ggcctagcca gagctttagc aaatgatcct gagatcttgc
      601 tgatggacga agcgttctcg gctcttgatc cgctgatccg gcgagaaatg caagacgaat
      661 tactggacct gcaagccaat gttcaaaaaa cgattatttt cattacccat gatttgaatg
      721 aagctttacg gattggggac agaatcgcct tgatgaaaga cggcgaaatc atgcagatcg
      781 gaacaggtga agaaatattg accaatccag caaatgatta tgtacgtgaa tttgtggaag
      841 aagtcgatcg ttccaaagtg cttacggctc aaaacatcat ggtacctgca ttgacgacga
      901 atatcgaatc tgatggacca aacgttgcat tgacaaggat gcgtaatgaa gaagtcagca
      961 tgctgatggc tgttgacaga aagagacacc tgaaagggat catcactgca gatcaagcgt
     1021 tagaagctag gaagcaaaaa cgtccattga tcgacttcct agatgaaaat gtgacggtga
     1081 ttgggaaaga catggtggta agtgatattt tcaatattat ttatgactca ccgactccac
     1141 ttgcagttgt agaggatgga aaattaaaag gtgtcgtcat ccgaggtagt gtgattgaag
     1201 cattagcaga aacaagcgag gtgagtgaac atgaataatc tattgaacta tcaattacca
     1261 gtggcaaact gggtagaaaa tatcacagaa tggtttacga caactttttc tggcctattt
     1321 tcatttttac aaacgattgg acaggcagtg atgtcgggga ttacaaattt actgttagtg
     1381 attccagctc ctttgtttat cttgttattg acggtagcag ctttctttat ttctaagaaa
     1441 cgtccaggac ttacgttgtt cacattaatc ggtctatggt ttatttataa ccaaggtcta
     1501 tggaatgatt tgatgaatac tgtcacactt gttcttttat ctagtgtgat ctcgatcatc
     1561 attggggtac cattagggat cttgatggca aaaagcagta aagcccaaag tattatcaaa
     1621 ccgatccttg actttatgca gacaatgcca ggattcgttt atttgattcc agcagttgca
     1681 tttttcggta tcgggatggt tccaggggta tttgcctcag tcatctttgc tctgccacca
     1741 accgttcgtt ttacgaattt agggattcga caagttccta aagaattagt agaagcttct
     1801 gattcattcg ggagcacaag tcgtcaaaaa ctattcaaac tagaattgcc tttagctaaa
     1861 agtacgatca tggcagggat caatcaaaca acaatgctgt cactttcaat ggtcgttatt
     1921 gcctctatga tcggtgcacc cggcttagga cgcggcgtat tatcagcatt gcaaagagca
     1981 caagtcggga atggatttgt caacggggtt gcattagtta tcttagcaat catcattgac
     2041 cgttttacac agcatttgaa ccagccaaac aataaaaaag ctgcaggagc aacaacacag
     2101 aaaagtaaaa aacgtcaagg actgatcatt ggtgctgtag tagtagtgat cttaggtgcg
     2161 attggtatag ggagtttttc atctgccaaa gaaacaaaac gtatcaactt atcttatgtt
     2221 gaatgggata cagaagttgc ttcaacaaat gtagtcggtg aagtattgaa acaaatgggc
     2281 tatgatgtaa cgatgacgcc attagataat tcaatcatgt ggaaatctgt ttcaaatggc
     2341 gagtcagatg ccatggtttc agcttggcta cctaaaactc atggttcaca atatgcacaa
     2401 tataaagatc aagtcgaaga cctaggtgcc aatttgacag gagcaaaagt tggtttagca
     2461 gtgccagcct acatggatgt aaattcgatc gatgagttaa ccgatcaggc aggtaagaaa
     2521 atcataggaa tcgagccagg agctggtgta gttacggctg ctgaaaacac gatccaaaaa
     2581 tatgacaact tgaaagactg gaaagtagag acatcttctt caggagcaat gaccgttgca
     2641 ctaggtcaag cgatcaaaaa acacgaacca attgtagtta caggatggac acctcattgg
     2701 atgtttgcca aatatgatct gaaatactta gaagatcctg aaaacggaat gggaagcgaa
     2761 gagcagatcc atacgatggt tcgaaaagga ttgaaagaag accagccaga agcttacaaa
     2821 gtattagaca atttccattg gtcagaaaaa gatatggaaa aagttatgtt ggaaatcaac
     2881 aatggaaaag atccacagca agcagcaaaa gactggatca aagagaacca agaattagtc
     2941 gaaagctgga aaaaataaaa agacagacac gaaataaatg gagcttccaa aattggatca
     3001 aatatccaaa tttggaagct ccatgttcgt ctctatcttt tttgtctata aaaaaagaga
     3061 atgcgtagct catcttcgaa acgtccaaag acgccgacaa actattatga atttatgggt
     3121 gcgagtcaaa gagatcgatc gtaggtatga aagactaatg tgattaaagt ttattatgta
     3181 cgaagtgcat tctcaaaaaa tattaaagaa tatacatagt ttatatactt aaaag
//
LOCUS       DAZKNM010000223         3204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000223 DAZKNM010000000
VERSION     DAZKNM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.229"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(553..2166)
                     /locus_tag="ACG8M9_002649"
     CDS             complement(553..2166)
                     /locus_tag="ACG8M9_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0379351.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..>3204)
                     /locus_tag="ACG8M9_002650"
     CDS             complement(2307..>3204)
                     /locus_tag="ACG8M9_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379352.1"
                     /translation="LTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAEF
                     AHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQE
                     ITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQ
                     LSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLA
                     QIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIH
                     HFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1031 a          549 c          571 g         1053 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taac
//
LOCUS       DAZKNM010000224         3201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000224 DAZKNM010000000
VERSION     DAZKNM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.230"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8M9_002651"
     CDS             301..585
                     /locus_tag="ACG8M9_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379353.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8M9_002652"
     CDS             588..1004
                     /locus_tag="ACG8M9_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379354.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8M9_002653"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8M9_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0379355.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2523..2609
                     /locus_tag="ACG8M9_002654"
     CDS             2523..2609
                     /locus_tag="ACG8M9_002654"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0379356.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2912..>3201)
                     /locus_tag="ACG8M9_002655"
     CDS             complement(2912..>3201)
                     /locus_tag="ACG8M9_002655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379357.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
     2881 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     2941 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     3001 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     3061 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     3121 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     3181 ttctttgccg tctatactct c
//
LOCUS       DAZKNM010000225         3167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000225 DAZKNM010000000
VERSION     DAZKNM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.231"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..403)
                     /locus_tag="ACG8M9_002656"
     CDS             complement(<1..403)
                     /locus_tag="ACG8M9_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379358.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGI"
     gene            complement(419..745)
                     /locus_tag="ACG8M9_002657"
     CDS             complement(419..745)
                     /locus_tag="ACG8M9_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0379359.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(758..2737)
                     /locus_tag="ACG8M9_002658"
     CDS             complement(758..2737)
                     /locus_tag="ACG8M9_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0379360.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(2758..3072)
                     /locus_tag="ACG8M9_002659"
     CDS             complement(2758..3072)
                     /locus_tag="ACG8M9_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0379361.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
BASE COUNT          914 a          666 c          501 g         1086 t
ORIGIN      
        1 aaataccgct tacaacaaga atgaaaacaa ataactgtcc tccattgtta tttgccataa
       61 aaggatacaa gaatgtaaat cctgaggtaa acaacactgg aatcactaat aaattaaaga
      121 cactctggaa caaaaaatac cactttgtcc atggaacgtt cgtttggtgt tttcgataaa
      181 gctttacttg ttctcctact gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac
      241 ttcctaaact tttagctgat agaagcagaa tcatccattg catgttcatg aaaaagaaaa
      301 aggcagtcag gtaacgtacg attagataac gattgaaata catgtttttc aaactaagtt
      361 gctttttttc taattcgatt ttccttttta ctgtatcctg cacaatgatc cttcctttct
      421 atttattttt ttgatacaaa taattcattt cctgtgcaac ttccaataat gtcatcgtcg
      481 tcatcagatg atcttgtgca tggaccatga taatttccat ttctattttt gtcccgctgg
      541 catattcttt cagaagttga gtttgtgact gatgcgcatg aagaatctct tcatttgctt
      601 gtcgcaactt ctcttctact tcttcaaaca tctctttacg catcgagcga aatgcttcat
      661 gtatcagtgt ccgtgcattt ccgctatgga aaatgatatc aaaagaagtc acttgtagtg
      721 tttcactgtt tattttttca gccatctgtt ttcctcctta ttcgatcagt ttgataaatt
      781 tactgctgaa attctcaaaa ataggttcag ctaagatttc ctgctgtact tttagctggt
      841 caactaacct aacgattgct ttagtcacga ccgtaattcc ttcattttcg acataagaag
      901 gcgaaactaa aaaaacaaaa tgaatttctt gatattgatc ccactgcatc ccagaaggaa
      961 tcaatgcaac ggctaccttt gtttcgattc caactggaac agcgggatgt gggactgcca
     1021 ctgtatcact gaatatgatt tgtcccatat tttcacgttg ctggatctgc tttcgcattt
     1081 ctcttatata tccttcttct tcatttatgc tcagcttttg cagcaaatca tgaagaattt
     1141 cttccttttt cggttcgtac gtataagtct tgaaaaattt ttcagacaac tgttcggtgt
     1201 aaatctgctt tttttgctgt agagaaaatg ctggtatcgg tctatctgag gaatgatagg
     1261 gtatagattc tccaactacc tttcgtattt tacgaacatc atcttcattc aaaaaaatac
     1321 tgacatgtaa aactggaatt ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca
     1381 ggtcgattcc ttttaaagtt gtttcattga tctcataata gccttttaca cttaccactg
     1441 taatattttt accgaattca ctgactacgc gattctttaa tagttgggca ctgccgtaac
     1501 ccgttgcaca gataatcaat gcatgtagtt tatgcgcagc tctttctttt tccaatgccg
     1561 ccatgagatg caaagccaaa taggcccatt catcttcatt gatggtatag tttttcaaag
     1621 ccggcatgtt gctgaaatgc ctttttactg cgtcaaagac tttgggattt tctttcttta
     1681 tctcctcagt caatgggttc tcaatgcgaa tacctcggct caatcgaatc atcatcggtg
     1741 acaaatgatc gagtagtcca ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt
     1801 cctgagacaa tcttttcaga actttcgcta attcctttga tagttcctga tcattttgat
     1861 tttctttgtg attcgactta gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg
     1921 gaaacgatac acctgcgatt acctcaatac gttgaacgat ccgctgcgcc acttgatact
     1981 ctactcgctt agaaacttct tgttcgatgc ctaattcctt gatttcaaac ccttctcgga
     2041 gccgtttgat ccccaaagcc aagtgtaaag tcagattctg tatgataaaa tccgaaactt
     2101 ttaacttagc ttctcttgtt tcatcgagaa tgataatgac tagctcttca aaactgatgt
     2161 ctgggaaaaa acggctttgc cctaaatatt ctttcaatga atttccataa tggtttccaa
     2221 aaaaagtatc catgataaaa tgccgtcgat ttctttcttc cccttcgatc catgtccctt
     2281 gaccatgctt agaaacgatt tttagtgaat atggaatcaa tcgctccttg atttcttgca
     2341 tgtcctttgt caaactggaa cgactgatat aaagctcttc tgccaaatca tccataaaaa
     2401 gaatggcatc ttccaaaagc aattggttca atatatactt ttgtcgatct tcggactcat
     2461 aaaatccttg cggttctcta gcatatccag gtactatttc ttgccgactc aaaaacaagt
     2521 caaatgtcag tctttgtaaa atatgcagtt ggtagccata acctggttta gcaatgatct
     2581 tacctccgtt tttttctact aactctttga tccgctgcaa gtaattacgg attgtccgat
     2641 cagataataa caattcagaa gctaaacgtt gactggtgac atactccttc ttgtgatcaa
     2701 tcaaatatct caacagcaat ttttccttct gtttcattat catccactcc tattccatca
     2761 tatgttctct tcgatcacct ctgctaactt ctggatgcct gtagggattg ggacatacgc
     2821 ttggaaaggg atactaacaa caggctttcc aacagattct cctaattttt tgaatttatc
     2881 caaaaacatc gtcgtttgcg ggctgatcag aaataaatca aaatcactat tcttaatcat
     2941 attatcgcct tctgtagctg aaactgcatc tacgagtacc tcttctcttt tttgcttaaa
     3001 gaaatccgtt gtctttgagg ccatcatcga ggatgacatt cctgccgcac aaataatcaa
     3061 tgcttttttc ataaatcaat cactcctttt atttttataa accaagcata tcttgtaagc
     3121 gttttctatt caaaatatat atttccgtag cattgcggaa aaaatgt
//
LOCUS       DAZKNM010000226         3042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000226 DAZKNM010000000
VERSION     DAZKNM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.233"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1053)
                     /locus_tag="ACG8M9_002660"
     CDS             complement(106..1053)
                     /locus_tag="ACG8M9_002660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0379362.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="ACG8M9_002661"
     CDS             1299..1763
                     /locus_tag="ACG8M9_002661"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0379363.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="ACG8M9_002662"
     CDS             complement(1825..2868)
                     /locus_tag="ACG8M9_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0379364.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         1009 a          603 c          581 g          849 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataagccg aaatttcaca aa
//
LOCUS       DAZKNM010000227         3038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000227 DAZKNM010000000
VERSION     DAZKNM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.234"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8M9_002663"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8M9_002663"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0379365.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG8M9_002664"
     CDS             complement(1034..1954)
                     /locus_tag="ACG8M9_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0379366.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG8M9_002665"
     CDS             2067..2819
                     /locus_tag="ACG8M9_002665"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0379367.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DAZKNM010000228         2984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000228 DAZKNM010000000
VERSION     DAZKNM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.235"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG8M9_002666"
     CDS             75..503
                     /locus_tag="ACG8M9_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0379368.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG8M9_002667"
     CDS             490..732
                     /locus_tag="ACG8M9_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379369.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG8M9_002668"
     CDS             complement(1032..1247)
                     /locus_tag="ACG8M9_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379370.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG8M9_002669"
     CDS             1354..1668
                     /locus_tag="ACG8M9_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379371.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG8M9_002670"
     CDS             1772..2950
                     /locus_tag="ACG8M9_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0379372.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT          979 a          451 c          610 g          944 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttct
//
LOCUS       DAZKNM010000229         2946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000229 DAZKNM010000000
VERSION     DAZKNM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.236"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG8M9_002671"
     CDS             complement(159..1469)
                     /locus_tag="ACG8M9_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0379373.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2839)
                     /locus_tag="ACG8M9_002672"
     CDS             complement(1481..2839)
                     /locus_tag="ACG8M9_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0379374.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
BASE COUNT          938 a          608 c          460 g          940 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataaga
//
LOCUS       DAZKNM010000230         2898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000230 DAZKNM010000000
VERSION     DAZKNM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.237"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..689
                     /locus_tag="ACG8M9_002673"
     CDS             <1..689
                     /locus_tag="ACG8M9_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0379375.1"
                     /translation="RNNVMFTLSLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIK
                     IVRSAYSENYQGANREYITILCKAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSE
                     WKEDLMAYISEKSDVYKPYLVTTKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPG
                     RNGGIQLASVKSLLLSIIKVKKEEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDT
                     QLDLFSYDTG"
     gene            1037..1207
                     /locus_tag="ACG8M9_002674"
     CDS             1037..1207
                     /locus_tag="ACG8M9_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379376.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1221..1838
                     /locus_tag="ACG8M9_002675"
     CDS             1221..1838
                     /locus_tag="ACG8M9_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0379377.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            1838..2776
                     /locus_tag="ACG8M9_002676"
                     /pseudo
     CDS             1838..2776
                     /locus_tag="ACG8M9_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         1054 a          436 c          583 g          825 t
ORIGIN      
        1 ggcgtaataa cgtcatgttt accctctctt tagcctactt tagttcaggc tattcaatcg
       61 aaacgtgcga atataatatg tttgagttta ataatcgatt agatcaaccc ttagaagaaa
      121 aagaagtaat caaaattgtt agaagtgcct attcagaaaa ctatcaaggg gctaataggg
      181 aatacattac cattctttgc aaagcttggg tatcaagtga tttaaccagt aaagatttat
      241 ttgtccgtca agggtggttt aaattcaaga aaaaaagaag cgaacgtcaa cgtgttcatt
      301 tgtcagaatg gaaagaagat ttaatggctt atattagcga aaaaagcgat gtatacaagc
      361 cttatttagt gacgaccaaa aaagagatta gagaagtgct aggcattcct gaacggacat
      421 tagataaatt gctgaaggta ctgaaggcga atcaggaaat tttctttaag attaaaccag
      481 gaagaaatgg tggcattcaa cttgctagtg ttaaatcatt gttgctatcg atcattaaag
      541 taaaaaaaga agaaaaagaa agctatataa aggcgctgac aaattctttt gacttagagc
      601 atacattcat tcaagagact ttaaacaagc tagcagaacg ccctaaaacg gacacacaac
      661 tcgatttgtt tagctatgat acaggctgaa aataaaaccc gcactatgcc attacattta
      721 tatctatgat acgtgtttgt tttttctttg ctgtttagcg aatgattagc agaaatatac
      781 agagtaagat tttaattaat tattaggggg agaaggagag agtagcccga aaacttttag
      841 ttggcttgga ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc
      901 gaagcgaaca cttgattttt taattttcta tcttttatag gtcattagag tatacctatt
      961 tgtcctaata tgattttagc agtataattg acttggtgaa taggtcattt aagttgggca
     1021 taataggagg agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt
     1081 ggctattttt aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc
     1141 tgataagcaa tttgaaattg aaaaagtaga tattgctgat tggttattgc aaccgagaga
     1201 attttagagg ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac
     1261 agaacttaga tcggcaatta caagcgttac agggcgtttc taaggtcttt tcagacaaat
     1321 taagcggtca atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag
     1381 gggatattgt tattgttact gaattagatc gattaggacg aaataataaa gaattaacag
     1441 aattgatgaa tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa
     1501 tgaatggtat tgaagatgaa aatttaaggc gtttgattaa tagccttgtc attgaattgt
     1561 acaagtatca agcagaatca gaacgaaaaa aaattaagga acgtcaggca caaggaatcg
     1621 aaattgctaa gaaaaaaggc aaattcaaag gtcgtcagca taaatttaaa gaaaatgatc
     1681 cacgtttaaa gcatgctttc gatttgtttt tgaatggttt atccgataaa gaagttgaag
     1741 aacaaactgg aatcaatcgc cgaacgttta gaaggtatcg agcaagatat aacgtgacag
     1801 tcgatcaaag aaaaaacaat gaaaagaggg atagttaatg agtacggtta ttttagctga
     1861 aaaaccaagc caggcattag cctacgcaag tgctttaaaa caaagcacca aaaaagacgg
     1921 ttattttgag atcaaagacc cactatttac agatgaaacg tttatcacct ttggttttgg
     1981 gcatttagtg gaattagcag aaccaggtca ttatgacgaa aagtggcaaa attggaaact
     2041 tgaatctttg ccgatttttc ctgatcgata cgattttgaa gttgcaaaag ataagggaaa
     2101 gcagtttaaa attgttgcag aacttctcaa aaaggcaaat acaattattg ttgcaacaga
     2161 tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc cataaagcaa atgccttttc
     2221 aaaagataaa acatttaaaa gactatggat caatagctta gaaaaagatg taatccgaag
     2281 cggttttcaa aatttgcaac ctggaatgaa ttactatccc ttttatcaag aagcgcaaac
     2341 acgccaaatt gccgattggt tgatcggcat gaacgcaagc cctttgtata cgttaaattt
     2401 acaacagaag ggcgtacaag gtacattttc actaggacgt gttcaaacgc ccaccttata
     2461 ccttattttt cagcgccagg aagccataga gaattttaaa aaagaacctt ttttcgaggt
     2521 ggaagctagt ataaaagtaa accaagggtc gtttaagggc gttctaagcc ccacacagcg
     2581 ttttaaaacc caagaggagc ttttagcttt tgtttcttct aaacaagcta aaataggcaa
     2641 tcaagagggg ataattgctg atgttcaaac caaagagaag aaaacgaata gtccgagttt
     2701 gttttcttta agtagtttgc aatcaaaagt caatcagctt tataaagcga cagcgagcca
     2761 aactttaaaa gctatttctt ttttaataac ttaaaaataa acttaatgta acagcaagca
     2821 cagtcaaggt atacaccttt gacaaaaaat agcacattct ctatcgaaaa tttttgctta
     2881 ttttttaaat tattttgg
//
LOCUS       DAZKNM010000231         2856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000231 DAZKNM010000000
VERSION     DAZKNM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.238"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..481)
                     /locus_tag="ACG8M9_002677"
     CDS             complement(119..481)
                     /locus_tag="ACG8M9_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379378.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(555..935)
                     /locus_tag="ACG8M9_002678"
     CDS             complement(555..935)
                     /locus_tag="ACG8M9_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0379379.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1017..2192
                     /locus_tag="ACG8M9_002679"
     CDS             1017..2192
                     /locus_tag="ACG8M9_002679"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0379380.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2269..2853)
                     /locus_tag="ACG8M9_002680"
     CDS             complement(2269..2853)
                     /locus_tag="ACG8M9_002680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0379381.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          889 a          554 c          481 g          932 t
ORIGIN      
        1 aaataaaatg cactaaaaaa ctatctttag cagaacattt cattgtattt tttggaccaa
       61 acaataaaaa aagaagtttg aaaaaaattc aaacttcttt gttcgcttaa tctgtatttt
      121 agaagaaagg cactccctct acttcgtaag ttccaagaac agagcgcatt tgacctggtt
      181 cttcccaaat atatccttcg cctaattctt ctctttgatt cttttgttca gtttcaaaga
      241 caaacaacgg aacattttcc tctttaatat gtgtatttgt caattccatc aaactaatct
      301 tgctaacatc gatatgaact atttctttta agaaattaag tatattcgct aaaccagtct
      361 ttccttcaga aagttccgct aacgcaaatt ccaacttgtc atcaattgaa tggaccaaaa
      421 attttttagt accatcctcc aaatgcaaca taattgtacc agcaactctt ttgcttgcca
      481 aaatttccac ctcgatcaac ttgttctatg gcttacataa aaattctagc acattttcag
      541 aaaaaataca ctttttattt cttattttct ttccgttcta gttcagataa tgcttcttct
      601 atccaaatag gtaactgctc cgcaatagtg gtaaatccga tcttacgatt tttattcgtg
      661 aaataccttt ttctgcgata tggaacagct ggatttgttt tttctaacgt tcttaaagat
      721 aaactgattt ttttggaata ttcatcaatg tcgatgatct gaactgttac tggctgtccg
      781 acttttaaca atgaatggat atttttcgta taaccggatt gtacttctga aacatgaata
      841 agtccttgcg tctcgttatc aagagaaacg aatgcgccat aaggctgtat ccctgtcact
      901 tttccttcaa ttatttgtcc aattttataa gtcatctact tccctctctt cgcatttatc
      961 attatactgt aaatagctca aaaaaagaag cgacgaaaat gactggaggt cttaagatga
     1021 tcgaatttga taaacagatt tcaagaaaag atacagaaag tgtaaaatgg gacgcaattg
     1081 ctgagacata tcaaatggat gacttacttc ctttgtgggt agcagatatg gacttcttgt
     1141 ctcccggtgg ggtagctaaa gcgtttagtg aatacatcaa acatggaatc tttggttatg
     1201 ccactctgtc tgataaacta tatcaggcta tcattcattg ggaacgccaa caccatgctg
     1261 tggagttgac gaaagaaaat atcgttttta caagtggtgt gttaaccagt ttagctgcag
     1321 cagtacaaac ttttacaaaa ccaggcgatt ctattttgat tcatgaccca gtttatcctc
     1381 cattttcctc tattatagaa acaaatcaac gtcacatcgt tcgaagctct ttgctagaaa
     1441 aaaataatca tttcatcatg gacttgtctg acatggagaa aaaaatcgaa gaaaacaatg
     1501 taaaagcaat gattttatgc aatcctcaca atcctggagg gcgtgtttgg agcaaagaag
     1561 aattgaaaca gttaagcgaa ttgtgtttaa aacatcaagt gattctgttt agtgacgaga
     1621 tccatcaaga tttaacttta ttcgatcata catttacttc catgctaacg attgatccta
     1681 atttagatga tctactagtt gcttttactt ccgcaaccaa gactttcaat ctagctgcaa
     1741 tcaaaaattc aatggtattt atcaaaaatc cagagatgaa agctgatttt gaaaaacatt
     1801 tagtgatgaa ccaacagcac gaaatcaaca cttttggact gattggaacc caagcggctt
     1861 atgagacggg tggagaatgg ttaagccaac tgattcctta tctagaaaaa aatgttcaaa
     1921 cagttaatca gttttttgaa cagtatcttc cgaaagtgag aataatgaac cctgaaggaa
     1981 cttacttaat gtggttagat tttaccgcct atatttcaga cgaccaagaa ttggagaaca
     2041 ctcttgttcg gaaaggaaaa gtcgtattaa atcctggcat cacgtttggt ccttccggtc
     2101 atgggcatat gcgcttgaat attgcttgtc cagaagaaac tttactggaa ggacttaatc
     2161 gaatcaagta tgctttagag acaatcgatt aaatcattta ttcaaaataa aaggacgaga
     2221 ccttactagg tttcgtcctt tttcataatt actccgtacc ataccaaact attctgaaat
     2281 ttcaattgtt tcaatcacta catcatgtac aggtcggtct tgtggaccac gttgtacatt
     2341 tgcgatttca tctactgtgt ccatcccttc tagtacatgt ccaaagactg tatgtcggaa
     2401 atctagccaa ggagttccgc cacctttata cgcttcaatg atttctgatg ggaatccagc
     2461 accttctagt tgtccaagca tattagctgg cacatttgtg ttatttacaa taaagaattg
     2521 gcttccgttt gtgtttggac ctgaatttgc catagataaa gctccgcgaa ggttgaacag
     2581 ttcacgactg aattcatctt caaaactttc gccataaata ctttcgccac ccattcctgt
     2641 tccagttgga tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa aaatcacgcc
     2701 atcataataa ccttttttgg aaagttcaat gaaattttga accgtcttcg gtgctagttc
     2761 agggaataat tgaacagtaa tgtcaccacg gtttgttttg ataacagctt ttggtccttt
     2821 ttcacttttt aaatctagtt gtgggaatgc catatt
//
LOCUS       DAZKNM010000232         2848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000232 DAZKNM010000000
VERSION     DAZKNM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.239"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..900
                     /locus_tag="ACG8M9_002681"
     CDS             4..900
                     /locus_tag="ACG8M9_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0379382.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            992..1207
                     /locus_tag="ACG8M9_002682"
     CDS             992..1207
                     /locus_tag="ACG8M9_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0379383.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1225..1497
                     /locus_tag="ACG8M9_002683"
     CDS             1225..1497
                     /locus_tag="ACG8M9_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0379384.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1499..2362
                     /locus_tag="ACG8M9_002684"
     CDS             1499..2362
                     /locus_tag="ACG8M9_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0379385.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1131 a          450 c          500 g          767 t
ORIGIN      
        1 ttaatgatac aatattacta tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa
       61 ctaaaaatac ttgaagaatt aagacgtatt ttaaacaata aaaatgaagc aattattatc
      121 ttgaataatt actttaaagg tggtgttgga aagtccaaat tatcgactat gtttgcttac
      181 ttgacagaca aattgaattt aaaagtttta atgatcgata aggacttaca ggcaacattg
      241 acaaaagact tagcaaaaac ttttgaggta gaattgccac gtgtcaattt ttatgaaggc
      301 ttgaaaaatg gaaacttggc ttcttctatt gttcatttga ctgataattt agacttgatc
      361 cctggcacgt ttgatttgat gttactgcca aaattaactc gctcatggac ttttgaaaat
      421 gaaagtagat tgcttgctac tcttttagca cctttaaaaa gtgactatga tctcattatt
      481 attgatactg taccaacgcc aagcgtttat acaaataatg caatcgtggc gagtgattac
      541 gttatgatcc ctttacaagc agaagaagaa agtacaaaca acattcaaaa ctatatttcc
      601 tatttgattg atttacaaga acaatttaac cctggactag atatgatcgg ttttgttcct
      661 tatttagttg atacggacag cgcaacgata aaatcaaacc tggaagaact gtacaagcaa
      721 cataaagaag ataacttggt tttccaaaat attatcaagc gaagtaataa agtaagtacc
      781 tggtctaaaa atggaattac agaacacaaa ggctatgaca aaaaagtttt atccatgtat
      841 gagaacgtat tttttgaaat gcttgagcga attattcaat tagaaaacga aaaagaatag
      901 aatcacaaat cacaagtgat taatcacaaa tcacttgtga tttgtgattg ttgatgataa
      961 aataagaata agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa
     1021 agcaaaacga aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt
     1081 tcgtgttcgt gctgacttac accatattat aaaaattgaa acagcaaaaa atggcggaaa
     1141 cgtaaaagaa gttatggatc aagccttaga agaatatata cggaaatatt tacctgataa
     1201 actttaaaaa ggagtgaaaa tgaaatggca gttacgtatg aaaaaacatt tgaaatagag
     1261 atcattaacg aattatcggc aagcgtttat aatcgagtat taaactatgt tttgaaccat
     1321 gaactagata ctaaaaatac tcgtttacta gaagtgaatc ttttaaatca attagaagtg
     1381 gcacaagaag ttgatttatt tcaacaacca tttgaagaat tacaagctat tcatgagtat
     1441 tggcggtcaa tgaatcaata ttcaaaacaa attttgacaa aagagaaagt ggcttaacat
     1501 ggcgaatatt actgacttca ccgaaaagca atttgaagat cgtttagaaa agaatgttga
     1561 acgactaact aaaaatagac tagcggttga atcgccaacc gcttttttac ttggtgggca
     1621 accaggatca ggtaaaacta gtttgcgatc ggcaatttcc gaagagacac aaggaaatgt
     1681 tgttatcatt gataatgata cgttcaaaca acagcaccct aattttgatg aactagtgaa
     1741 actttatgaa aaagacgtag taaaatacgt taccccttat tctaatcgca tgacagaagc
     1801 gatcataagc cgtttgagag ataaagggta taatttagtg attgaaggta caggacgaac
     1861 aacagacgtt cctattcaaa ccgcaacaat gcttcaagcc aaagattatg aaacaaaaat
     1921 gtatgtcatg gcagtaccta aaatcaactc atatttagga acaattgaac ggtatgaaac
     1981 tatgtatgca gatgatccaa tgacagccag ggcaacacca aaacaagcgc atgatattgt
     2041 tgtcaaaaac ttaccgacca atttagaaac ccttcataaa acgggcttat ttagcgatat
     2101 aaggctttac aatagagaag gagtgaaact ctattcaagc ttagaaacgc cttccattag
     2161 tccgaaagag accttagaaa gagaattaaa tcgtaaagta tcagggaaag aaattcaacc
     2221 gactttggag agaatagagc aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt
     2281 taaagcaatt caacaaaaaa tggaaagctt gcagccacct acaccaccaa tacccaaaac
     2341 acctaaactt ccagggattt aaaccttaaa aaagtaaaag agtagttacc aaaaaacggt
     2401 aactactctt tttttataaa aacatttcct cattttttaa aagctgatcg agaaataaag
     2461 cacacaactg tattctttct tttttcagtt ggtacatttt gcagtctgta acgtgccatt
     2521 tactgtttgt taccttaaca tatccagtcg aaaacacaag ggtatataaa cgagcaagct
     2581 cgcccttgtg ttttctttgt gtctatgttg cgttctcgtc acagcaaacg acacgaacac
     2641 actggcgaaa atgaaccaac aaaacgaaaa aagaaaggag cgtacactgt aatttatttc
     2701 tagcttttaa aacatgagga aaacagcttc acttcctcaa acgcctaaca aaaatggttg
     2761 tcaaaaaact aaaaatcaaa aaaagaaaag aggaaaagaa aatggaaaaa acaaaaaaat
     2821 tacaattaga agattttaca gaaaatgg
//
LOCUS       DAZKNM010000233         2842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000233 DAZKNM010000000
VERSION     DAZKNM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.240"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..873
                     /locus_tag="ACG8M9_002685"
     CDS             58..873
                     /locus_tag="ACG8M9_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379386.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            999..1256
                     /locus_tag="ACG8M9_002686"
     CDS             999..1256
                     /locus_tag="ACG8M9_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379387.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1508..2080)
                     /locus_tag="ACG8M9_002687"
     CDS             complement(1508..2080)
                     /locus_tag="ACG8M9_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0379388.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2096..2701)
                     /gene="fic"
                     /locus_tag="ACG8M9_002688"
     CDS             complement(2096..2701)
                     /gene="fic"
                     /locus_tag="ACG8M9_002688"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0379389.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          920 a          523 c          443 g          956 t
ORIGIN      
        1 gactacaagt aaaaaatact gtatgatcat gacagattca agaaaggagc aataagattg
       61 gttaagatag caggactttc taaaggtgga aaaaccgttt cacaacttta taaacaaaac
      121 tacagagact ggagacagtt cagaaaaaac attaaggatt cttactttat tctaccaaca
      181 gaacttgaaa aatatttacc gaataacaaa gctattaatt tatatctgta ttactgtttt
      241 aaggcaaaga atgaaggcgg ggattcctgg cattcggtca ataccatagc agagtctcta
      301 ggagtgacaa ctaaaactat aaacacctgg aataaaacct tagttgattt aggtatgatt
      361 gcacgtatag acgataacca tctcagtaaa tcgacaattt tacttcctct ctctagttac
      421 tataaattag cccttgatag ttctttaaag gatgttaccg agagcactgt ccatgaaatt
      481 gatggcaact tggttgcagt ttatcatttg tttgaatgga gaaaaaatga ggctgatgaa
      541 aattataacg ttccttataa cacactctgt tgtgttttta aaagaaaaac acaaaaagat
      601 actatctata aatttatctt ggtccaagat gagaaaataa gtaatttcga attaagcact
      661 ccttctagta agatttatga cgatgcatat agatttgaat gtcaagatct agaagccctc
      721 ctttccgcaa ataatctaaa caatatagaa catgaaaact ttgttgtaaa cactagattt
      781 aatttagttt ctgaaaagga ctcagatttg ttagatttgc tgatagagct tactaaaaat
      841 ctagataaga aagaaaacat tactgtacta taactagcat cggtccatca ttaagtttgg
      901 actattagaa cacttgctat tacagaaaaa ggtatgctag ccacgcttac tatttattgg
      961 tatcaaacaa gtatcttaat ttgaaaggaa gatgatttat ggctaattta tatatagaga
     1021 acaaaggaga cttgaatgcc attattactt taatcgtagt tatggtaaaa tcaataggta
     1081 atatcacaat aggaatgctg tttattatat tagcagcagt gctgattttt atgatagtta
     1141 tttggttctt aaatgaaaga tctaaagaca atcaacaccg tagggatatg gagataaaat
     1201 caatagagtc aatagattat ttatcgataa taacactaaa aaagccacgt tcctaaacgt
     1261 gacttacgct gaacgatatg tgttgtttgg tagacgaaca aatcgttcat tataaatata
     1321 tgatggtatc ctaatatagt aagcgtggct agcatacctt taattacttt accttttaca
     1381 gttaccatac acattttttg agcagaaatc aagtaaataa aaaaatatta tatttcacaa
     1441 catgctaata gactcatatc tagaacgcat taagtattta cctgtctggt tcgctgagac
     1501 ttctttttta actttcttca attttcttct ttattctaaa aagcgtactg cgactgaaac
     1561 cggttatagc ttcaacttcc ttatacgatt tacctgaagt aagcagttca tatgcgtgcc
     1621 tcttcttcgg tgtgattgtt gctttcggac gtccttctct gaaattagga ttattctttc
     1681 ttgcaaacat ttttccttct tgggtacgtt ccacaatcat atcacgttcc atttctgcta
     1741 cactcaacaa cgtccgtgtc accattcgcc ccatggacgt attttcaatc gttcctagat
     1801 tcaaaacctt gatcgttacg ttatcatcga gtaaaggttc aatgatctct aatgcttctc
     1861 tcgtgttacg agcaaatcga tcaagcttcg taacaactat ttcatctcca ggcttcagac
     1921 cagccaacag ctgattgaat tttggccgat cccgttttgt acccgtaaat ttttctgaaa
     1981 aaatattctt cttctctacg ccgttctcaa gcaactgttg aatttgttct tttgtacttt
     2041 gagaagcggt tgagacacgt gcatatccat atttcgtcat ttggtccacc tacttttaca
     2101 aatcatctat cgtatactca gaataccctt catagaaata gctgacatcg attcctttca
     2161 tatataactc gcgatcgtca attttatcag tcagagcatt ggaaattaga tagcgtattt
     2221 ccaaatcgtt tatgggactt cgctccattg cagaaagata gtctgcttta tcgattaagt
     2281 tccaatctac aactttttgc aattcttcct ttaaaatgag atccagccaa attctagtac
     2341 ttcggccatt tccttctcga aatgggtgag cgatattcat ctccacatat ttcttgataa
     2401 tctcttcaaa agaagtttgt ggcatctgat caatatgctg taacgaatgt tctaaataca
     2461 taactggtgc aaatcgaaaa tttccttttg caatattgac cgatcggatt tttccggcaa
     2521 aatcgtaaat atccgaaaat agaaattcat gaatttcaga taaaccttta aatgtaccaa
     2581 cttcgatttc atttattttt ccacaatcgt acaattcttt cgctttttgt ttactgagtt
     2641 tttcttcaac cttagccaac tcaacttgat tggttaatcc caatttattc tctaaaatca
     2701 tgcgaaatct ccttaacttt atgacataag tttatgaaac actctcaacc ccattatatc
     2761 gcgattttgt ttatgtgtca aaaactatta agtttctgac ccccgataga tttttcaagt
     2821 gtctctattt tgcacagtct ag
//
LOCUS       DAZKNM010000234         2776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000234 DAZKNM010000000
VERSION     DAZKNM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.241"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..1556
                     /locus_tag="ACG8M9_002689"
     CDS             231..1556
                     /locus_tag="ACG8M9_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0379390.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1549..1899
                     /locus_tag="ACG8M9_002690"
     CDS             1549..1899
                     /locus_tag="ACG8M9_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379391.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1881..2066
                     /locus_tag="ACG8M9_002691"
     CDS             1881..2066
                     /locus_tag="ACG8M9_002691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379392.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT          976 a          440 c          538 g          822 t
ORIGIN      
        1 ttttctaata taactaagca aaactggata atacgacact tattttcaaa ttttcacaag
       61 actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac aaatcaacag
      121 aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc gattcttctt
      181 tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt atgatggatt
      241 attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt tacgcaagtg
      301 tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc atgagtcgag
      361 cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag aagaagtacg
      421 gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa acgttacatg
      481 tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac aatattttca
      541 gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg atcttaaaag
      601 tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg agaaagaagc
      661 tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct acagtaggtg
      721 tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa cataatgatg
      781 gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat attcctgaaa
      841 tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa attggtatca
      901 tgagtattaa agacttagct aattggaatc catacactat taagagccat ttaggtgtga
      961 ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata gcgatcccac
     1021 cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga gattatacca
     1081 ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct attcgtatca
     1141 ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg atcttagaaa
     1201 cgagaccagg tttttcacac cagatgaaga tccctataac agataataca aaggaattgc
     1261 agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta aggcacgttg
     1321 gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta tttagtgatc
     1381 cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat
     1441 atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt agtattacta
     1501 gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat gattgattat
     1561 aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac tgaaaaaatt
     1621 gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat gacgactgaa
     1681 gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg tattcaaaaa
     1741 gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat cttaggtagt
     1801 gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat acgtcatatc
     1861 gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag caatatgagc
     1921 taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc cagcgtttga
     1981 taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca ggataccata
     2041 aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt tatgttttac
     2101 attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa aaaaactagt
     2161 tacaacctta ggacgttgta actagcttga agcatactag agctaactaa aatagcacta
     2221 gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag cttattcagc
     2281 taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac tacgaataac
     2341 gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac taaacattgc
     2401 gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag ctttatgatg
     2461 ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa ttctcctttt
     2521 taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc atttgattga
     2581 gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt ataacataaa
     2641 tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca aacgatttat
     2701 tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat gtttgttaaa
     2761 accaccatta cctgta
//
LOCUS       DAZKNM010000235         2689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000235 DAZKNM010000000
VERSION     DAZKNM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.242"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..515
                     /locus_tag="ACG8M9_002692"
     CDS             171..515
                     /locus_tag="ACG8M9_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0379393.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ACG8M9_002693"
                     /pseudo
     CDS             516..935
                     /locus_tag="ACG8M9_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="ACG8M9_002694"
     CDS             complement(1027..1377)
                     /locus_tag="ACG8M9_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379394.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2673)
                     /locus_tag="ACG8M9_002695"
                     /pseudo
     CDS             complement(1343..2673)
                     /locus_tag="ACG8M9_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
BASE COUNT          996 a          487 c          522 g          684 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttcttttt
//
LOCUS       DAZKNM010000236         2632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000236 DAZKNM010000000
VERSION     DAZKNM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.243"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..1215)
                     /gene="recA"
                     /locus_tag="ACG8M9_002696"
     CDS             complement(166..1215)
                     /gene="recA"
                     /locus_tag="ACG8M9_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0379395.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1324..2565)
                     /locus_tag="ACG8M9_002697"
     CDS             complement(1324..2565)
                     /locus_tag="ACG8M9_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0379396.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT          689 a          591 c          469 g          883 t
ORIGIN      
        1 gagaaacttt tctattaaat tattctttgt agatgaacag atatttttat tttatgtacc
       61 cactcgtata attaaataac tcacgatttc tttattcaat aaaaaaacgg caggtcaagg
      121 agagtaccta ctcttcagac cagccgtttt tgtttatcat aatttttatt cttctactaa
      181 aggcaattct tcttgtcctt tttcatcttc tgggacagcg acttcttcac caataccata
      241 agctgctcta actagagcag aaacttctgc catcatttca ggatgattag ccatatagat
      301 tttagcgttt tcccgtcctt gaccaatccg atcttcttta taagaatacc aagcaccgct
      361 tttatcaaca atgtcttttt ctactgccat gtctagaagt tctccttctt gtgagatccc
      421 taaaccatac atgacatcta cttcagcgat tttgaaagga ggtgctactt tgttttttac
      481 gactttgatc tttgtacggt taccaacaat atctgtacct tgtttcaatt gttctgcacg
      541 acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc
      601 tggattcgca aacatcacgc ctactttttc acggatttgg ttgatgaaaa tagcgattgt
      661 ttttgttttg ttgatcgaac cagagagttt acgcaatgct tgagacatca aacgtgcttg
      721 taacccgaca tgtgagtcac ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc
      781 aactgaatcg actactacga tatctacagc cccacttgat actaaagcat cagcgatctc
      841 tagaccttgt tctcctgtgt caggctgtga aagaagtagt tcatcgatat tcacacctaa
      901 tttttgtgca tattgcggat ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc
      961 atttttttgt acttctgcaa tagcgtgtag tgcaactgtt gttttacctg aactttcagg
     1021 tccatatact tcgatgatac gtccacgggg gtaaccgccg acacctaaag cgacatctaa
     1081 cgcaagagag ccacttggaa ttgtagagat ttgttggtcg attttttcgc ccaatttcat
     1141 aatagagcct ttaccatagc tcttttcaat ttttttcagt gcagcatcta aggctgcttt
     1201 acgatcatct gccaatacga taatcctcct tatatttaca aatcagagca acatttcatt
     1261 atccttatca tatctttttt agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat
     1321 ttatcatttt cttaaaagct gttttctcaa aagatcaaat ccttgcataa ccgcattata
     1381 tctgatagca gcacgatctc tggcaaagtg atatttttcc gaaaccacgc cgtctttaga
     1441 tgaaatagcg atccatactg ttcctgcaga ttgaccttct aacgtgtccg gtccagccac
     1501 tccggtgaaa gctacactgt aatctgttct tgctatctca cgtgcttgaa tggccatctg
     1561 ctccacacat tctttgctga ctgttccata ttctgctaat agctccggat caatctccag
     1621 aaaaccagct tttgtttgga gcgagtaggt aacgaatccg cctggaaata cttcagatac
     1681 accagcaatg tcgcctaatg cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt
     1741 tacagtctgc ttgttttctt tcagtagttc cactacgact tttgctagcg aattatcatc
     1801 gccgtatcca taaaaatatt cccctactcg ttcttggatt ttttcttcta atgctaaaag
     1861 agcttgattc cctgcctgaa catcatttgt tttcactgtc agtcgtaatg tcacttcatt
     1921 tggtttggca tatggtgcga tcgttggatt gatctgtgtc tcaatcagat cttttagctc
     1981 agtgaccagt cgcgattcac caatcccata aaagcgcaaa actttagaaa ttagtttttc
     2041 ttcagaagga aaacgttcct ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa
     2101 ctcattaggc ggaccgggaa gaaggatata cgcatgtgta tctgtttgat aaaaagtccc
     2161 taaagcaagc cctgtacgat ttggtagagg aaccccgcct tcgataatct gggactgttg
     2221 cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa tatgccaata attttttgta
     2281 tccttccgta ttttgtatga gagacttacc gaggtgagcc gcagttgctt cttttgtcag
     2341 gtcatcttct gtcggtccta atccgccaca taagagtatg agttcacttc ttgtttcagc
     2401 tagagcaatc aactcttcta aacgctgctg attatctcct acaactgttt gataataaac
     2461 ttcgatacct agatcagcta gctgttctga caagaaagtg gcattcgtat tcacgacttg
     2521 tcctagtaaa agttctgttc cgacagctat tatttctgct ttcattactt cccgctccct
     2581 tatatagtat acgctgtatt tttatatttt tttatttaaa aagagggcaa gg
//
LOCUS       DAZKNM010000237         2623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000237 DAZKNM010000000
VERSION     DAZKNM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.244"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            431..817
                     /locus_tag="ACG8M9_002698"
     CDS             431..817
                     /locus_tag="ACG8M9_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0379397.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            911..1102
                     /locus_tag="ACG8M9_002699"
     CDS             911..1102
                     /locus_tag="ACG8M9_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379398.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1616..1948
                     /locus_tag="ACG8M9_002700"
     CDS             1616..1948
                     /locus_tag="ACG8M9_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0379399.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1858..2052
                     /locus_tag="ACG8M9_002701"
     CDS             1858..2052
                     /locus_tag="ACG8M9_002701"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0379400.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2091..2237
                     /locus_tag="ACG8M9_002702"
     CDS             2091..2237
                     /locus_tag="ACG8M9_002702"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379401.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
BASE COUNT          951 a          425 c          431 g          816 t
ORIGIN      
        1 atttttcgtt tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt
       61 gtctattcga actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat
      121 ctatacacga ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa
      181 aaagctcaag acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa
      241 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
      301 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
      361 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
      421 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
      481 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
      541 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
      601 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
      661 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
      721 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
      781 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
      841 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
      901 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
      961 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
     1021 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc
     1081 gaaattcgaa attaaagaat aactcaacta agggagtctg tgacataagt aaaaagatag
     1141 tttcaaattt cgaacaataa atttgaaact atcttcttta taattataaa gtatcgattt
     1201 tacagcgttc tataatgatt catagaatct aaaacgaatg attttaatta tcaacgtttt
     1261 ttaattcgtt tttagcttaa aaataacttt tgtcacagtc tcactcctaa aattacttta
     1321 tttacgtatg aagtgggcag gaggtcgaat ccagaactgg gctatggtac ttcaacgagg
     1381 acttctcctc tagaattgaa aagcatgcga tttaccttcc gtaacgacgg tagatttatt
     1441 caactatgtg tccaatcaaa atttgtctga tttcaaaaat agtcaagaga attttggttg
     1501 ttaaaaactc ttatctatca acttccacag tttagttgac aaaccctgat actaagttgg
     1561 gctatgatac acttttcttg actatcagtt acgttaccgg aaggaatgaa ctctattgag
     1621 aaaaacatat gatcgtaaat ttaaactaaa gatttcacag gatatccttg agaaaaaagt
     1681 aactaccaag aaaattgctg aagaatacaa tatctctcgt ccaacaattt cccgatgggt
     1741 ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga caaggaaaaa gattacctga
     1801 taaagcagat ttctacattc ttgaacagga aaataaacga ctaaaagagg aagacgagtg
     1861 ttctacgaac acaaagatcg atacggatct ccagggatca ctcaaaaact ttacgatgaa
     1921 ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc gtgaaatgaa tttatgcaca
     1981 agaggatttc atcgacgcaa atcttctcat ggtagagaaa aaacaatcaa agaaatggta
     2041 aaaaaatgtt gaatcgataa tttgaacaga accaaattga tgctgtttgg gtgatggata
     2101 ttacttacat tccatgtaat gacggtcgcc tctatttgtc cacctatatt gacttagcaa
     2161 ctcgtattct acgttactat atggtagatt cacacatgaa aaaagagatt gtcgtccgtc
     2221 cattacaaat ttattaagga gaattgccta cggtaattca ttccgataga ggtagccagc
     2281 atcggtcttt tatttatcaa gagctgttga acgaccacca catcacacat tcagtgagcc
     2341 aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag
     2401 aattaatcga ctccaacaaa tataaaagta aaatagagat gaaggtgtta atccaagatt
     2461 atttagaaga ctactaccca aacaaaagaa tccatacaaa atttatgatg actccttgac
     2521 aatttgaagc gaagaaaata agggatctat aatatctagt gttggtgacg ccttctgttg
     2581 ccaatacttt ttcttttttc taataaacgc aaacagacac ctt
//
LOCUS       DAZKNM010000238         2591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000238 DAZKNM010000000
VERSION     DAZKNM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.245"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..297
                     /locus_tag="ACG8M9_002703"
     CDS             4..297
                     /locus_tag="ACG8M9_002703"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0379402.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(375..1487)
                     /locus_tag="ACG8M9_002704"
     CDS             complement(375..1487)
                     /locus_tag="ACG8M9_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0379403.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            1640..2488
                     /locus_tag="ACG8M9_002705"
                     /pseudo
     CDS             1640..2488
                     /locus_tag="ACG8M9_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          932 a          476 c          418 g          765 t
ORIGIN      
        1 ttagtgagta tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt
       61 gcagaagaaa tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag
      121 aaatatgact attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg
      181 aaagatgaag aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa
      241 ctccgtaaaa agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa
      301 gatacacatg gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt
      361 ttattcgatc tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt
      421 tcagtcaccc caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg
      481 atcgttactg ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa
      541 ttaaaaactg ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata
      601 ccgacccagt taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat
      661 gagccaatga ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta
      721 gatacaatag aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct
      781 cctgcaacga tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc
      841 gaatacatgc ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga
      901 tgttttctgt aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt
      961 tgtgtaaaga aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat
     1021 gaaaagccgt acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg
     1081 ctgcttagta aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca
     1141 tacggtgcta ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc
     1201 ttataaccaa agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat
     1261 ggtttatgct tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat
     1321 acgatgaata atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa
     1381 gctgcttctc gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa
     1441 tccacctgtt gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg
     1501 taaatactat ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca
     1561 tgtttttata aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac
     1621 tgagagaacg atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa
     1681 aaatttgatg attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt
     1741 tgctgcagtt ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga
     1801 tggaaatagg aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga
     1861 ataatataat catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa
     1921 ctgtcgaagc cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa
     1981 tcatgtcaga cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc
     2041 gaaaagttgt taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta
     2101 tttaccaaag tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct
     2161 cacgtttccg ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta
     2221 atcgagaaaa tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca
     2281 ctaatcctaa agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca
     2341 ccaaacatgt tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc
     2401 ctttcaagga ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc
     2461 cgacttgttt ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg
     2521 atcgctgatt atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg
     2581 caaaaaacac t
//
LOCUS       DAZKNM010000239         2582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000239 DAZKNM010000000
VERSION     DAZKNM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.246"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(177..656)
                     /gene="pssE"
                     /locus_tag="ACG8M9_002706"
     CDS             complement(177..656)
                     /gene="pssE"
                     /locus_tag="ACG8M9_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0379404.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(673..1134)
                     /gene="pssD"
                     /locus_tag="ACG8M9_002707"
     CDS             complement(673..1134)
                     /gene="pssD"
                     /locus_tag="ACG8M9_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0379405.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(1148..2398)
                     /locus_tag="ACG8M9_002708"
     CDS             complement(1148..2398)
                     /locus_tag="ACG8M9_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0379406.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT          788 a          543 c          372 g          879 t
ORIGIN      
        1 ataaagattt aaatattgca tttccaccct tataactcgg tataaaagtc atctcgatat
       61 tctttttttc ccaattaaaa ttgagcattt gtgtaattat acttgtaatt ccacctttta
      121 ccgagctgtc attaccaatc ataagtactc tttgcataac gctttactcc ttatatttat
      181 attttttcta ttaatacctc tatcgaatta ataatcgttt ccgtgttaga tacatacttg
      241 ttaaacatcc gaactcttgt ttctttaacg gcatcttcta aattttctaa ttcataacaa
      301 ggatagataa tattcatctc atcaaattgt ctaattaact gcagttggtg atcatcgaca
      361 tgctcaccat acttagccaa tcgaggaaca gcaatgaccc ttttaccttt ttttacggcc
      421 ccaattatag cacctgtacc tccatgagta atcaccattt ctgctttttg catagtttca
      481 gtaaactcat ccctatttag aaatgcttta taggaataat ttagtggttt ataatcacta
      541 taaccactct gtgcaaatgt actttcgatt attactcctt tttcaattaa atcatcaata
      601 gcttttaaca atctatcaaa ttgaaatttt tgtgaaccta atgttataaa tatcattttt
      661 ttctcctatt atttaatata tcccacccaa ataaatagca ttaggataaa attgtagcat
      721 agtttcccac tgaacataaa attgatctgc aaaatgatac atcaattttc ctgtctgagt
      781 agggctggtt acttttgcaa acgattcaat ataaatcagt ttttttccca tcatttttgt
      841 tattaagcag atagggatca tagctaaaac acctgtacaa atcacgagat ccggtttttc
      901 ctttatatat ataataattg atttgaataa attaataagt atagtacaca aaaaaaactt
      961 ttcttttcta tttacttgtc tcatgtagta cgttttttcg ccttttgact cagctgaata
     1021 ttctgtctgt tctgttataa caaaactctc atacttctcc atcagtggtt ttaacatcat
     1081 taactgttca taatgccccc cagaggaagc cgcaaaacat atctttttgt tcatattaac
     1141 ctctctatca gtcacgctga aaaatatctc tagtgtaaac tttttcttta atatcatcca
     1201 agcaggtatc atatcgattg gcaataatag aatcacacat cgccttaaac tgctctaaat
     1261 cgttgataac cttacttccg aaaaaagtct ctccatcttt cagtgcaggc tcataaatca
     1321 ccacagtcgc acctttagct tttacgcgct tcataactcc ctggattgaa ctctgacgaa
     1381 aattatcact gttgctcttc atggtcagcc ggtatatacc gattaaaact ggattttccc
     1441 tgttgacatc atactgatta tattcagcgt agttgtaata gcccgccatc tgaagaacac
     1501 gatctgcgat gaaatcctta cgggtacgat tagattcaac gatagcctca atcaaatttc
     1561 caggaacatc tgcatagttt gccaacaact gcttggtatc ttttggcaag caataacctc
     1621 catatccaaa ggatggattg ttgtagtgag ttccaatacg aggatcgaga caaacaccat
     1681 tgattatctg ctgagtattc agccccttca tttcagcata agtgtctaat tcattgaaat
     1741 agctgactcg cagtgctaaa taggtatttg caaacagctt tactgcttcg gcttcagtga
     1801 atcccataaa aagagtatcc acgttctcct taattgcact tttctgcaga agtgttgcaa
     1861 aaacatgagc agcatttaac aagcgctcat cagtcatatc agtaccaaca ataatacgag
     1921 atggatacag attatcatat aaagccttgc tctcgcgcag aaactctgga ctaaaaataa
     1981 tgttcttact ccccgttgtc tcacggactg attcagtgta gccaacagga attgtagact
     2041 tgatgaccat gatggtatca ggattcacgt ccatcacaac ctcaataact gactcaacag
     2101 cagaagtatc gaaatgctga gtacgactgt catagttagt cggtgtagca ataacaacga
     2161 aatctgcgga tttataagcg ctccttgcat ccaaagtagc agttaagtca agttcttttt
     2221 ctgtaagata cttttcaata tattcatcct gaattggaga cttccgttta ttaatcagtt
     2281 caatcttttc agggataatg tctaccgcgg ttacctggtg atgctgcgcc aatagtgtgg
     2341 cgatactcaa accaacataa ccggtgccgg ctactgcgat gttgtacttt ttcttcacaa
     2401 tatctcctct ttcctctctt aagtatggag aatgttactc ctacggctat tcatcatgtc
     2461 aaaataacac agcttagaat gttgataaaa taattttcca gaatgtgata aaatctcttt
     2521 catttataaa ccacacatct aattttatct attctccaca agacatgtag agtattcttt
     2581 aa
//
LOCUS       DAZKNM010000240         2506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000240 DAZKNM010000000
VERSION     DAZKNM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.247"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG8M9_002709"
     CDS             14..1141
                     /locus_tag="ACG8M9_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0379407.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG8M9_002710"
     CDS             complement(1359..2501)
                     /locus_tag="ACG8M9_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0379408.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          834 a          456 c          410 g          806 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttac
//
LOCUS       DAZKNM010000241         2501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000241 DAZKNM010000000
VERSION     DAZKNM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.248"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..214)
                     /locus_tag="ACG8M9_002711"
     CDS             complement(<1..214)
                     /locus_tag="ACG8M9_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379409.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPE"
     gene            complement(733..1218)
                     /gene="vanZ-A"
                     /locus_tag="ACG8M9_002712"
     CDS             complement(733..1218)
                     /gene="vanZ-A"
                     /locus_tag="ACG8M9_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0379410.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1371..2282)
                     /locus_tag="ACG8M9_002713"
     CDS             complement(1371..2282)
                     /locus_tag="ACG8M9_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0379411.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          758 a          468 c          365 g          910 t
ORIGIN      
        1 gttctggttc tgttggctca tttgggatta aatccactac tagcgttgag ttagttaact
       61 ttgcaatttg ttctagtgtt tttatggttg gatctgattt tcctgattct attcgtgaat
      121 aatttgatct actcatttct aattcttggg agaatttttc ttttgatttg gtacctctca
      181 tcttttttaa gctttctcta aatgctagtt ccaagtttct cccccctctt tttgtgtaat
      241 aaaattcacg ctttatttaa attatagctt ttaatatctt aataaacaag tgattaatca
      301 cttgtgattt gtgattattc caaaagtagg taatttcacc aacctttttc aaaagttttt
      361 atcgacgatt attggactgg atcgtgtttc ctaaagcgat tctaagcgca tgtttaattt
      421 taaagtgggt aattctcttt aaaacgtcct acaaagattc aaaaaaagca tggctgctag
      481 tgggggtacc gtcaggaaaa tgcggattta caacgttaag gaaatttatt aaagagacca
      541 attcctcagc actaaaattg ataattgggc tttctttgtt actatagtgt taggtaagct
      601 ttttacttta tcaccggttt tcccctcact tcacacctac gggcgagttt ccccacatcc
      661 cgttttccat ctaatccaat tcattcaata tctttatgtg tatgaaagat cagattgctt
      721 gatttagaca tcttacacgt aatttattct taaatgggta cggtaaacga gcaataatac
      781 gagcaaaagt atacctacaa aaataataac tctgtctaat tttttttgat tcatatgctt
      841 attgcttaaa ccatataatt tcagtccaag aaagcctcca acagtatttg taattacatc
      901 tgttatgtct gtcgctccaa tagcgaagat aaattgaatt atttcaaaag taagacttaa
      961 aaccagtaca aaagcaaact taggtaaaaa tccgatttct ttaaaattga cattcaaaag
     1021 caagccaaat ggaataaaga ttataacatt atctatcatc tctctgaaat tcccagtagc
     1081 agtaaatgga gtcaagttaa gacttctttg atgataatta aatactgata aaatattgta
     1141 ttgtaatttg aataacacta accagattag tgtcactaaa tataaagcta gcaatcctct
     1201 agataatatt tttcccaata taatttcctc taattctttt gtttaagttg tatcattagt
     1261 atagcaatgt cctagttaac gtgggtttta aattaaaaag ctaacgttac cccaaaagtg
     1321 ctagtacaat aatgctgttc attaatttcg ttggtttcat tccgccatcc ttacctcctt
     1381 gaattagtat gtgttgatcc gggaaacact gtcacaatta caccgtctat attgtttcct
     1441 gatatctcat aacgaagatt agtcggcaca tgaatggtgg tatttttagt aacaggataa
     1501 taaaagatct catatttttc cccatttaca ctaacagaaa tggttttttc ttcttttagg
     1561 taatccatat attcctcgag aacgaaattc ttttctttca taatcgcact atgtggtaaa
     1621 ccaacatagc gaatatgcca tggttcatat tgaattcctg ttaactctgt tttgtcctct
     1681 ggataacgta aaatgaaccc gtatttccaa gcattttctt ctatccactt tccttcaggg
     1741 gctcgttcca ttttcgtcaa gcttgatcct acatctagtg ataaacctga attatgctca
     1801 ctataacctg ctggtaaggc atactcagcc cccatttctt ggtaaagcac actttgctca
     1861 tcaaagtctc gatagccact attaataata aaatgactaa cgccaccctt tacagcatca
     1921 ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca tataaatatt actatcaagc
     1981 aacccgtatc catttattaa ttcgtcatgt ttagataaat tcacgatatc tgacttcaca
     2041 ctttcttggc gaacaggata tttactattg attaatagca gatttccttg ataaacctgt
     2101 tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc cactattttc taaatgttct
     2161 ttgggatttt gatcataatt ttgaaattcg actttttcct gagaaaacag tgcttcatta
     2221 acgtagtcat aacctaagta tattaagaat aacaataaca ataaaaaaaa caacttcttc
     2281 attttcagtc tcctccttaa ctatatagtt ggaagtatga aatatttcta gaatcttccc
     2341 cttaaacggc acaatctcgt ttgagattgt gccgttttgc cccgtaaatt acagtcaatt
     2401 atttcgccct actggattta ctattatcac caatgtagat gaattttcat aagtgatacc
     2461 catgctgaat ttaacgaggc tgaactgcct gttgacaatg g
//
LOCUS       DAZKNM010000242         2441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000242 DAZKNM010000000
VERSION     DAZKNM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.249"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..284
                     /locus_tag="ACG8M9_002714"
     CDS             <1..284
                     /locus_tag="ACG8M9_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HFQ0379412.1"
                     /translation="ISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQI
                     PYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            412..1095
                     /locus_tag="ACG8M9_002715"
     CDS             412..1095
                     /locus_tag="ACG8M9_002715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0379413.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(1250..>2441)
                     /locus_tag="ACG8M9_002716"
     CDS             complement(1250..>2441)
                     /locus_tag="ACG8M9_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0379414.1"
                     /translation="KGVQAQFIKGRLDDSPSHCEHCGSIRIIRNGSYTTRTQMLKVKE
                     KLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKK
                     YFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLF
                     TILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIV
                     QHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFK
                     RPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKK
                     KFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
BASE COUNT          735 a          448 c          535 g          723 t
ORIGIN      
        1 aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag
       61 ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc
      121 ccatctttca gattccatat attggactag tcctttcaaa aacggcattt gcatggattc
      181 aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg
      241 tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata
      301 aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca
      361 gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat
      421 gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa
      481 aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca
      541 gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa
      601 cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc
      661 ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa
      721 aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca
      781 gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca
      841 aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg
      901 gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc
      961 aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat
     1021 ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa
     1081 acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct
     1141 caattcgaac ttgatgtaaa agtggctctt cgtcaaattg tgttggcgaa gacaaatttg
     1201 aataaaactt agtccgatat gcggcgcgtt ctgtgcgccg catatttttt tagttttcaa
     1261 agatcagacc ttgttgtaaa tagatccgtg cccgaaagtt ataaaagttg cgataaccaa
     1321 acgcaattcg tttaatcagc ttgatcttgt tattcagccc ctcagtgacg ccgtttgagt
     1381 ggggcacctt aaaggcattt gaaatacccg attggtagcg atggaagatc gtgaatttct
     1441 ttttaaacga ctgaggtaac gtagatgaaa ttgttttaag ggtttcttga aagccttccg
     1501 tatctcgttt aacgaaataa tagcgtaata attgacaaaa atgatacgca gttcgcaatt
     1561 cttcgttgta gcttaacagc tcatcaacga tttctgtttg actaattggt cgtttaaaca
     1621 gtcgatgata atgccgtttg agtggctcca atgtcgtgga atctttgagt aagagtttcc
     1681 aatatcgctt gagccgacgg tacttcttct gattttcaga gtgtgaggtt cgaaaacgat
     1741 tcatgatctg gacacgtaat tgattgaagc tgcgattgat gtgttggaca atatgaaagc
     1801 ggtcagttac gatctccgca tttggaaaga ctgttttaag caactgacaa tagctggcat
     1861 tcatgtccat cactaggtac ttgaccttta aacgggcctt tctaggaaag cgcaggaagt
     1921 attgagttag tttgtaaagt ctgcgatctt caaggatcgt aaatagctga ttggttgtcc
     1981 catcaacaca aataaaactc atggaaccag aaacagactt cattgatttg aactcatcca
     2041 tacacaacac cgtcggaagg aagttcatac gtggctggta atgcttcgtt gcttcacgca
     2101 agatccgtgt caccgtccca tccgagacga agtacttctt agcgattagt ttacgtgatt
     2161 gattttcgta cagttccatg agaatcgcct gcttcagttc ctttgttagt tgatgatgtt
     2221 catcaactaa gtccgttttc gcagaaaagg tccgtccaca atcatggcag agaaaacggg
     2281 tacgttttaa ttctaaaata gtcaactttt ctttgacttt caacatctga gtgcgtgtcg
     2341 tataggaacc gttgcggata atacgaatcg atccacagtg ttcacaatgg gaaggtgagt
     2401 catccaatct ccctttaata aactgcgctt gaacgccttt a
//
LOCUS       DAZKNM010000243         2418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000243 DAZKNM010000000
VERSION     DAZKNM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.250"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG8M9_002717"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG8M9_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG8M9_002718"
     CDS             1320..1778
                     /locus_tag="ACG8M9_002718"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0379415.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG8M9_002719"
     CDS             1937..2407
                     /locus_tag="ACG8M9_002719"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0379416.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          699 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaa
//
LOCUS       DAZKNM010000244         2409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000244 DAZKNM010000000
VERSION     DAZKNM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.251"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..927
                     /locus_tag="ACG8M9_002720"
     CDS             175..927
                     /locus_tag="ACG8M9_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0379417.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1067..1990)
                     /locus_tag="ACG8M9_002721"
     CDS             complement(1067..1990)
                     /locus_tag="ACG8M9_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0379418.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2005..2277)
                     /locus_tag="ACG8M9_002722"
     CDS             complement(2005..2277)
                     /locus_tag="ACG8M9_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379419.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          798 a          474 c          405 g          732 t
ORIGIN      
        1 gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga
       61 tttacctttt atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc
      121 ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag
      181 tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
      241 gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc
      301 aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg
      361 gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag
      421 catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa
      481 cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg
      541 tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa
      601 tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg
      661 tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa
      721 gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct
      781 tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc
      841 gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta
      901 aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta
      961 aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg
     1021 tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa
     1081 acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt
     1141 ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca
     1201 aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg
     1261 ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc
     1321 ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag
     1381 atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac
     1441 ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt
     1501 ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt
     1561 cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac
     1621 gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct
     1681 ctttttcttt ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg
     1741 cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca
     1801 ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta gggatataat
     1861 cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc
     1921 cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa
     1981 tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac
     2041 agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg
     2101 cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac
     2161 tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac
     2221 aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc
     2281 aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt
     2341 gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat
     2401 tgaatacat
//
LOCUS       DAZKNM010000245         2397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000245 DAZKNM010000000
VERSION     DAZKNM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.252"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            206..2068
                     /locus_tag="ACG8M9_002723"
     CDS             206..2068
                     /locus_tag="ACG8M9_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0379420.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="ACG8M9_002724"
     CDS             2068..>2397
                     /locus_tag="ACG8M9_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0379421.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAZKNM010000246         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000246 DAZKNM010000000
VERSION     DAZKNM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.253"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8M9_002725"
     CDS             316..570
                     /locus_tag="ACG8M9_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0379422.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8M9_002726"
     CDS             571..888
                     /locus_tag="ACG8M9_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0379423.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8M9_002727"
     CDS             1447..2382
                     /locus_tag="ACG8M9_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379424.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKNM010000247         2387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000247 DAZKNM010000000
VERSION     DAZKNM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.254"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..940)
                     /locus_tag="ACG8M9_002728"
     CDS             complement(2..940)
                     /locus_tag="ACG8M9_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0379425.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1431..2387
                     /locus_tag="ACG8M9_002729"
                     /pseudo
     CDS             1431..2387
                     /locus_tag="ACG8M9_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          821 a          418 c          388 g          760 t
ORIGIN      
        1 tttacttcca aaacctaaat tcacgttgcc aaaaatcaat ctgcttttgc aattgttttt
       61 cgttcgcttt taaagtcgat ttcattaatt ccgttaaatc aattggagat atttctctaa
      121 tcaatttttt aaatttagtc ttagtattct tacttagctt tccccacata ctttcttcat
      181 gcaacaaagt ataaaccata gcttgctcat taattttttc taaagtagcc cacgcaggtt
      241 tcaagatgtg taaatcatta aaacaatcat tccagtaatc aaccatatct ctttttaatt
      301 caacttctac acgccataaa tgttcagaca caacttcaac atctgcgtta tctttacgtt
      361 cttgtttttt attataaatt ctaataaatc tatcactatc acggacacca aaatattttg
      421 tttctggctt gccattacga ccataaaaaa cagttttctt aactgcttta tcagtcattg
      481 catagtaatc gctcaaatca tcttcaaaat caaaagctaa gtctaatctt gtaaaaccgt
      541 catcttccat gtagtcgata atattttgtt ttaaccaaat catttcttca tgtgtgagtt
      601 tattgggatt aaattcaaca cgcatattac gtctatccca agtatctgct tttactttgt
      661 catattcgat ataaactttt tcttgaagtg ccttagcttt aaactttgtt tgaagtatat
      721 cccaaagtcg tatttgtggc tctacactca taaagtcaga tagcttttta gcattagttt
      781 tgttcaaatt tccaacgatt gtcatggcat caaaacttaa tgcgggttga gattttccca
      841 aagtttgacc acttaaccgg ctattactta accggctatt agagacggcc ggttttttta
      901 gtgagatttc tgcagctaat ttttcatcaa ttttcttcat tttttatcgc tcctaaaata
      961 atttttggaa cgaaaaaagg acgagtggaa tcgcaaaata aatttcgcct gtatccattc
     1021 gtccattttg ttttgtattc aaaatcaaaa attgatatac taatcatcga atgaataaca
     1081 agtgtggttt gttattccgg gcgagaacgt tggtagcgtt gctcgtcttt ttttatgtct
     1141 ttttttcgtt aaaactattt taaaactaaa tatatcttaa agcaaataaa ttcaaggggc
     1201 taaaagcgcc ccttaaaaac cccttgcctc tgaaaagttt tgtgtttcct ttccgtcggt
     1261 ttcaaaagct aatttactca aaaaaataga tcaagggtaa atgcactcac tgcgttcgcc
     1321 cttgagctat tttatttcgc aaattttaac gcatttgccg acgaaaagat aaaacacata
     1381 aaaatatacg cagatatatt taaaacgtct tagaacgcaa aattttacgt ttaaaagcta
     1441 tgcaaggact gtatgaagca aaattattga gttatccaag aacagataca ccatttatta
     1501 cagagaacga atttgcttat ttaaaagcga attttggcaa atatagcggt tttttaggac
     1561 ttgatcttga aatggttcaa acagagccta gaaagcgtta tgtggacggt agtaaggtac
     1621 aggaacacca cgccattatc ccaacaaaac aagtacctac cgaatctgca ttagcgaaaa
     1681 tggacgattt acaacgaaaa atttatgctt tagtcgttaa aacgaccgtt gccatgtttt
     1741 tacctgatta cttgtatgaa gaaaccaaga tacaaacgaa agtagctgat ttgctgtttc
     1801 aatcgatcgg aaagacacca aagcaagaag ggtggaaaat tcttttcaaa caacaaacca
     1861 aagaagaaaa agaggacgtt caaacgttac cactcgttat cattggcgaa cgtgccgaag
     1921 ttggtgttaa gagtgttgaa aaagaaacgc aaccgccaaa agcttttaca gagggtacat
     1981 tattaactgc tatgaaaacg gcgaataaaa cggttgatga tgaagaagca atcaagattt
     2041 tacaagaagt tgaggggatt ggaacagaag cgacaagagc aagtattatt gaagcgttaa
     2101 aacaaaaaga atatatccaa gtgattaaga ataagcttgt tgtaactgaa aaaggaaaat
     2161 tattgtgcca ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg acggctaaat
     2221 gggaaacgta tttaaaaaaa atcggtaaaa gagaaggcaa tcaagagaac tttattacga
     2281 atatcaaaaa attcattgtt catttactgg aagctgtacc taacgatata gaaaaactaa
     2341 atttttctga ttaccaggaa cagaaagaaa aagaagcaga aaaaagt
//
LOCUS       DAZKNM010000248         2380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000248 DAZKNM010000000
VERSION     DAZKNM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.255"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            247..1356
                     /gene="pdhA"
                     /locus_tag="ACG8M9_002730"
     CDS             247..1356
                     /gene="pdhA"
                     /locus_tag="ACG8M9_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0379426.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1359..2336
                     /locus_tag="ACG8M9_002731"
     CDS             1359..2336
                     /locus_tag="ACG8M9_002731"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0379427.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT          782 a          452 c          507 g          639 t
ORIGIN      
        1 aaaatagaaa aaatgcagat gaaatataaa acgctttcct tattgtgatt ttattcacta
       61 atgctgttaa tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc
      121 ttgtatttct cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt
      181 tttattcact gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg
      241 tgacaaatgg caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat
      301 gcagatttcc cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta
      361 gtacctgatt tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt
      421 gtattagacc aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca
      481 actgctggac aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt
      541 cttcttccag gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa
      601 gcttttttat ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct
      661 ctaccaccac aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt
      721 ttgaagaaac gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca
      781 caaggtgatt tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt
      841 tatatccaaa acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa
      901 acacttgcac aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat
      961 ccgcttgcag tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga
     1021 ccagtcctaa tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat
     1081 ccaacccgct accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc
     1141 cgcttccgta aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc
     1201 atcgaagcaa ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa
     1261 caaaaggttt cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa
     1321 caaattgcaa tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc
     1381 aagcaatcac agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg
     1441 gtgaagatgt tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt
     1501 tcggagaaga ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt
     1561 tcggtcttgc gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt
     1621 tcgaagcaat ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca
     1681 ctagaaatct acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat
     1741 tgcattcgga taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc
     1801 cttccaaccc ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg
     1861 ttgttttcct agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag
     1921 cttacgaagt tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta
     1981 tcacatacgg agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa
     2041 atatatctgt tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca
     2101 ttcaatccgt ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg
     2161 gcgttgccgc gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc
     2221 caatcggacg agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct
     2281 ggttgccaaa tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa
     2341 agaagggacg aaacaaagtc atggcttatc aatttaaatt
//
LOCUS       DAZKNM010000249         2379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000249 DAZKNM010000000
VERSION     DAZKNM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.256"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..2232
                     /locus_tag="ACG8M9_002732"
     CDS             79..2232
                     /locus_tag="ACG8M9_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0379428.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          812 a          422 c          529 g          616 t
ORIGIN      
        1 tccgcccatt ttttcttttt tattaagaag tttttgggta caatgaagca tgaatgtaaa
       61 taggcggaag gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg
      121 ttagaaaaaa taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat
      181 gttccttttc gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt
      241 gctagattgg gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct
      301 acttctacga ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga
      361 aaagctttag ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt
      421 acggctaaag atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta
      481 gatgaaaatc tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg
      541 aaagaagcca caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat
      601 agtgcgatgg tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa
      661 attgctacca tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa
      721 aactctggtg ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca
      781 ggagtttcga ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt
      841 attcttggca gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac
      901 ctagcaaaag gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat
      961 gaagatgtcc tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat
     1021 gtttcgaatc aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg
     1081 aatgcagaat tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc
     1141 aacaacggca aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca
     1201 ggagaagtgc tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag
     1261 aatgctttag gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact
     1321 ttgacagcag gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg
     1381 attcgtctgc aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct
     1441 cttgatgcag tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta
     1501 aagatgttag gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct
     1561 aaagcttatc agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg
     1621 atcgatttgc cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc
     1681 aatgcggata atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga
     1741 cagtttgaca catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc
     1801 gggaaacgag tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac
     1861 ttgggtgaag tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca
     1921 gaaaatatgg cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac
     1981 acaacagcca aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca
     2041 gaaacggttg cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac
     2101 ggaccaacag acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa
     2161 caagaccaca taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc
     2221 atggcaaaat aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt
     2281 ttgtgaaaat agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt
     2341 ttgaacaccg tttactcggc aaaaaaggtt gtgacaaaa
//
LOCUS       DAZKNM010000250         2361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000250 DAZKNM010000000
VERSION     DAZKNM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.257"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8M9_002733"
     CDS             <1..375
                     /locus_tag="ACG8M9_002733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379429.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8M9_002734"
     CDS             complement(324..893)
                     /locus_tag="ACG8M9_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0379430.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG8M9_002735"
     CDS             complement(967..2256)
                     /locus_tag="ACG8M9_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379431.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
BASE COUNT          664 a          479 c          408 g          810 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt c
//
LOCUS       DAZKNM010000251         2352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000251 DAZKNM010000000
VERSION     DAZKNM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.258"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>2352
                     /gene="esp"
                     /locus_tag="ACG8M9_002736"
     CDS             26..>2352
                     /gene="esp"
                     /locus_tag="ACG8M9_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0379432.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          854 a          370 c          481 g          647 t
ORIGIN      
        1 attattaagg gagttgattt gaataatggt tagcaagaat aataagagag tatttctaga
       61 aaaaacaaag aaacgagtat tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc
      121 tgttttgatt ggtgttggtc ttgtcttggg aacaactgaa ctagttaaag cacaagatga
      181 aatttctcca agcagcccac ttgaaacagc gataagctcc gtacaagtag gtgacaaagt
      241 agcttcgggg aatacttttc aggagaatcc tggttatacg aaaaattata acttttcgga
      301 tttacaattt agtcctcaag aactaactgg agacacacta aaaggcaata caattggatt
      361 tgaagtttac ggaaaacata atattgcagc atcaactaaa aactgggaaa tccgtcttca
      421 attagatgaa cgattagcta aatatgtaga aaaaattcag gtcgatccaa agaaaggaat
      481 tggatcttct agacggacct ttgtaagaat taatgattcg ctgggtagac ctacaaatat
      541 ttggaaggtc aattatattc gtgcaagtga tggattattt gctggggcag aaacaaccga
      601 tacgcaaacc gctcctaatg gggtaattac gtttgaaaag agcctagatg aaatttttaa
      661 agagataggc atagacaatc tcaagaccga tcgtttaatg tatcgaatct acttggtaag
      721 tcatcaagac gatgataaaa ttgtaccggg tattgatagt acgggctact ttttaacaga
      781 ttcagatgat ttctataata gcttagatgt atctgaaaat aatcctgacc agtttaaaca
      841 tggatcagta agtgcaaaat atgagaaacc taatactcaa acaaaagatg gttcaggatc
      901 cacaggtgct aatggagcta taatcttaga tcataagtta acgaaaaatt ataatttctc
      961 atattcatct tccgcaaagg gcacgccttg gtatgctaat tacaaaattg atgaacgatt
     1021 agttccatat gtagcgggta tacagatgca catggttcag gctgacaaag taacatatga
     1081 tgtttcgttt gaatccggaa caaaagtagc tgatttagca atcgagcgac gggaaggtca
     1141 tgaaaattat ggcataggtt caataactaa taatgattta actaaactta tcgattttgc
     1201 taatgcaagt ccacgtccag tcgttattcg atatgtttta caattgacta agccattaga
     1261 tgaaatctta gaagatatga aagcaacagc acaagttgaa gaaaataaac catttggtga
     1321 agatttcatc tttgattctt ggttgtcgga tacgaataaa aaattaatcc agaacactta
     1381 tggaacaggt tattattatt tgcaagatat tgatggtgat ggaaaccctg acgataaaga
     1441 agagagcgga gacacgaatc catatatcgg gaaacctgaa ttagaagaag tatatgatgt
     1501 tgacacaaca gttaagggga aagtattcat ccacgagtta gcgggaacag gtcacaaagc
     1561 ccaacttgtt gataaagaag gtactgtatt agcagaaaaa actatcgctc caaatgaaaa
     1621 agatggggct ccaatttcag atactgtaga atttgaattt acgggtgtag attcaagtaa
     1681 attaatcgcg aaagatgaat taaaaatcca aatcgtttct ccaggttttg ataaaccaga
     1741 agaaggttca accgttatta aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt
     1801 tggatttaaa ccagatgcta aagaatcaat tcggaataat aaaaacttac ctgaagatgc
     1861 agagtattca tggaaaacag agcctgatac ttctaacgtt actgatagta cgaaaggtat
     1921 tgtaactgtt aagatcggaa atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc
     1981 ttctcaagct atggaaaatg atgcaacata cgtacctata acaacaactc cagaaacgac
     2041 agttcaaagt ggaaaaccta catttgataa accagatgtt cctttagcta aagatgcctt
     2101 ttcaatttca gatgtctata ataagtactt cggtaatgca agtgttgatg caaacactgg
     2161 tattgttaca ttcactccag ctaaaggtgt aggagaatcg gagccgatta ctggaacaat
     2221 tcctattaaa attgtttacc aagatggttc tgtaggcacg accgatttag cagtaactgt
     2281 aagtaaagat atttatgaaa atccaggaga aaacattcct gcaggctacc acaaagtaac
     2341 cttcaccgca gg
//
LOCUS       DAZKNM010000252         2311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000252 DAZKNM010000000
VERSION     DAZKNM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.260"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..1148)
                     /gene="galU"
                     /locus_tag="ACG8M9_002737"
     CDS             complement(267..1148)
                     /gene="galU"
                     /locus_tag="ACG8M9_002737"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0379433.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1191..2210)
                     /locus_tag="ACG8M9_002738"
     CDS             complement(1191..2210)
                     /locus_tag="ACG8M9_002738"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0379434.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          597 a          523 c          365 g          826 t
ORIGIN      
        1 ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
       61 ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat
      121 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
      181 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
      241 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
      301 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
      361 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
      421 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
      481 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
      541 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
      601 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
      661 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
      721 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
      781 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
      841 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
      901 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
      961 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
     1021 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
     1081 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
     1141 accttcactt gctgttcctc ctaaacgacg attaactatt gatcatctat ttaacgaaga
     1201 gaaaattcat tctccatttt tccgtcacgt aacatgattt cttttgctgc ttttttgata
     1261 tcttctccat tatacaaaac attatagatt gtttgagtaa ttggcatctc aacacctagc
     1321 tgttgagcta gctctactgc tgcttttgtc gttgccacac cctcaacgac cattcccatg
     1381 ttttctagaa cttcttccag cttatgtccc tgtcctaaca agttaccggc acgccaattg
     1441 cgagaatgaa cacttgtaca agtaacgatc aagtccccca caccactcaa accgataaat
     1501 gtcagaggat tggcacccat cgcgacacca agacggctga tttctgccaa tcctcgagtc
     1561 atgattgctg cttttgcatc atctccgaat cctaatccat ggatagcgcc ggcaccaatt
     1621 gcaatgatat ttttcagtgc tgcaccagtt tctacgccaa tcacatcatt atttgtataa
     1681 atcctgaaat aatcattcat gaacagtttt tgtacatagg ctgctgcttt ttgatcaata
     1741 cttgcagctg taattgttgt gatatcatgt actgccactt cctcagcatg gctaggacca
     1801 gataatacaa caattgcctg tcttttttct tctggaattt cttcagccaa aacttctgag
     1861 attcgtttat gcgtatcttg ttctagtcct ttacttgcat gtataatgat tggcttagta
     1921 ttcattttac caataagttc ctgtgcgact gaacgaatcg cttttgttgg aacaacaaat
     1981 aaaatagcat ctgcgttatc cactgcttct gccaattctt tatatccgat aatcgattct
     2041 gggatcgtca aatcaggtaa aaaatgctga tttgtatgat acttattgat ttcatcaatc
     2101 tgttcaggta catttcccca aatacggaca tcatgcccgt tttctgccaa tgtttgtgct
     2161 aaagcagtgc cccaagatcc aggaccaaga actgcgattt tttgtttcat ggatattcct
     2221 ccaaataaat aattgataat ttccattata gtatacattt cttaaagttg ggactaattt
     2281 ccattgtttt taagtgtttg tttacttctt t
//
LOCUS       DAZKNM010000253         2227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000253 DAZKNM010000000
VERSION     DAZKNM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.261"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..280)
                     /locus_tag="ACG8M9_002739"
     CDS             complement(<1..280)
                     /locus_tag="ACG8M9_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0379435.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="ACG8M9_002740"
                     /pseudo
     CDS             742..2124
                     /locus_tag="ACG8M9_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DAZKNM010000254         2188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000254 DAZKNM010000000
VERSION     DAZKNM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.262"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1912)
                     /gene="uidA"
                     /locus_tag="ACG8M9_002741"
     CDS             complement(104..1912)
                     /gene="uidA"
                     /locus_tag="ACG8M9_002741"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0379436.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1935..>2188)
                     /locus_tag="ACG8M9_002742"
     CDS             complement(1935..>2188)
                     /locus_tag="ACG8M9_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HFQ0379437.1"
                     /translation="RIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYIN
                     TYDGKQETLEILVDKLMGLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT          692 a          443 c          270 g          783 t
ORIGIN      
        1 aaaaatattt atcattcttt aaacagaaaa tctttctata aaaaacaaaa aaaaacgatt
       61 aggctaggct aatcgttttt tgaacattct taaaaaatcc aaattatttt ttataactga
      121 aattgggtat tgatgaccat cgtttctgta gatagtaagc aatcatttta ggttttctat
      181 ctctagtaaa gattcctttc ttatttcctt gcactcttaa aatcccttga cttgttgcaa
      241 aatctgcaaa attccacact tgttcaccaa caaaattttc acatgtatct attacttcgt
      301 gatttgcttc gtaataagcc acttgatact cttctgtaaa cattgttggt actgtatcat
      361 gaaatccagc tactgtatct gctccatatt ctgtaaacat tattggcgta tcaggacatc
      421 tttccatcca tccatctagc tcttttttca acagatattt tgcatgtgcc agatcccctc
      481 ccgaaacata ccaaccataa taacgattta aacataaaac atcgacaata tctcctacct
      541 tacaaacatc tggcgttgat tttagataag taacgattgt tacaggtctt ttctgcggat
      601 caagttcttt agttaattca actaaaggtt caaaatattc tttagcacct tcggaatccg
      661 aatctggttc atttgcaata gaccacatta caacacttgg gtggttcttg tcgcgttcta
      721 tcaattcctt gatcaccgtt ttatgagaat tttctgttcg tatctctttc cacgtattat
      781 gtttttccac attatcatct aataaagtgg ccatgaaatt taaatgtaac cctactgcag
      841 gtacttcatc aataactaca ataccttctc gatctgctaa tcgcataatt tcttctgagt
      901 aaggataatg tgatgttcta aaagaattag cacctatcca tttcattaaa ttaaaatctt
      961 taatatttac agcctcattc aaaccacgtc cattaacata cgaatcctca tgcttaccaa
     1021 atcctttaaa ataaaaaggt ttattattaa tcaaaaattt gccattggta acctgaacac
     1081 tgcgaatacc aaattcttct gtataggtat cgatcacttt attatcatca attaaactaa
     1141 ttttcatact atataaataa gcgtctagag gttcccaaaa aattggattc ttaagctgaa
     1201 tcattccttt ctttccttca gcttcagcta caagttgagc ctcttcgtca tatatcgcta
     1261 ttaagatttt tccggtccat tctgtatcga tattgaattc tacacttgca tccaaatcag
     1321 ttattcttgt attaatagta atatcactta tatagtcttt cggagttgta tataacttaa
     1381 caggtctatg aatacctgca taattgaaga aatcaaaatt aactaagttt tcctttttta
     1441 tatccccatt ttcctgtact gtttccttca taatccccac aggtaaagta ctatcgtcaa
     1501 taatattgtt tacagcaact gtaattcgat tattcccgtc ttttacaaac cgattaattt
     1561 cagactcaaa tggtgtaaac ccacctttgt gttgagtaat aaactttcca ttcaaaaaaa
     1621 cttttgcttc atgtgttgca gatccaaaac gaagaactaa tctttcttct aataatcttt
     1681 taggaagagt cacttctttt tcataccaca cccatccaac atgatttcta atgttctctg
     1741 tttcaaataa atcattataa gaagagggaa ctgaaatcgg ataactctct ttttcattta
     1801 ttggttctaa agcaagttct tcctgtaagt catcatttgt atctaattta aatctccaga
     1861 gaccgcttaa gtcaattact tgcctcgtct ctgtcataat gggatacaac atataaacca
     1921 ctcctatcta aatattaaat aaatgtatcc tttaaaccac aataagcatc tattggactt
     1981 actcccttaa attcagaaag acccattagt ttgtccacca atatttctaa agtttcttgt
     2041 tttccatcat aagtattaat ataagtcttt acttgaggta catcagccaa atgaaacgga
     2101 cactgtacag atacaaaaat tgttggaatt tcatgaatgt aaaacggtat atccggagtt
     2161 cctttacttg ctggccagac aattcgtt
//
LOCUS       DAZKNM010000255         2173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000255 DAZKNM010000000
VERSION     DAZKNM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.263"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1504
                     /locus_tag="ACG8M9_002743"
     CDS             <1..1504
                     /locus_tag="ACG8M9_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0379438.1"
                     /translation="RNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAGLNNLFTSIFQ
                     MLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVFTLGLLCIPFL
                     RFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVINVSMLITFTF
                     QFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVGELEKETIDDI
                     IKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIMSIFAIIYESC
                     IAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVGSDNLMSI
                     SFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILVNWFGLYG
                     VLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVASYLSYFACSY
                     VNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLVKR"
     gene            1682..1903
                     /locus_tag="ACG8M9_002744"
     CDS             1682..1903
                     /locus_tag="ACG8M9_002744"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0379439.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          723 a          229 c          418 g          803 t
ORIGIN      
        1 aaggaatata attactggaa caatttttaa gttatatcag attatcattc ccttttttat
       61 gcgtacactt atgatttatc agatgggaat ggagtatgct ggattgaaca acctgttcac
      121 atctatattt caaatgttaa atttagcgga attgggaata ggtgctgcac taactgtaag
      181 tatgtataaa cctatagcag aagacaatag aaaagagatt tgtgctttac tgaacgcata
      241 taaaaagtac ttttcaatta ttggatggat agtgtttaca ttaggattgt tatgcattcc
      301 ttttttgaga tttttagtta aaggttcgat tcctggaaat ttaaatatga cagttttgta
      361 tttaatgtat ttgataaata ctgttttgtc gtattggtta ttttcatata gaaagagtct
      421 aatatatgca catcaaagaa atgatgtaat taacgtgtct atgctaatta cttttacatt
      481 ccaatttgta atgcaggcat atgttctgat tttcttaaaa aattactatt tgtatttatt
      541 ggcttctatt ttgggacaaa tagttcttaa cttaatgtgt gctgttgttt ctcaaaagat
      601 gtatcctgat tatactcctg ttggtgagtt agagaaagaa actattgacg atattataaa
      661 aaaagtaaaa ggattagtta caaacaaggt aggcggtaca atattacgat cttcagattc
      721 aattattatt tcatctttct taggtctttc agtacttgcg atttatcaga attattactt
      781 tattatttct gccataatga gtatatttgc aataatttat gaatcatgta tagcaggtat
      841 aggtaacagt cttattgtag aaagtcaaga aaaaaattat atagattttt gtgatattac
      901 aacaattata ggttggtttc tggcaattag ttgtgcatgc tttattacat tatttcagcc
      961 gttcatgatt atctgggtgg gaagtgataa tcttatgagt atttcatttg ttatatttat
     1021 gatagttcat tttttcttat atgaaattga tcagttaatt gggttataca aagatgctgc
     1081 tggaatatgg tacgcagaca ggtttagacc attaattaca gcaatgataa acattgtatt
     1141 aaatatcatc ctcgttaatt ggttcggatt atatggagtt ttggcggcaa ccattgtttc
     1201 agtattgttg gttgatttac cctggttatt atataatgtt ttccaaaata tatttaaatt
     1261 tgaatcggca ggtaaatatg tttggaagct cataagtttg tgtgtaatag cttttgttgc
     1321 ttcttattta agttattttg cttgttcgta tgttaatatc ggaaatttca aagtgataag
     1381 tttagtgata aatttagtta tatcggttgt catatcgatt ttagttattc ttctgtttaa
     1441 tatccgtaat aagaagttta agtatatttt gaaagttctt acgtcggtat tggtaaaaag
     1501 atgagaagag acatcagaat gatatataaa gtaactacaa tttttaggct gccgaatagg
     1561 cgaacgatgt atagtttttg ttccaacgaa gactcttgtt gaaattggag atgatatgaa
     1621 ggagttacaa ttttgactca tgtttatgat tgatcggttt taaaagtagt atggagaagc
     1681 tttgggatca tcggagagga tcaaaatagg taataatgtt tttataggaa tgaagtctag
     1741 aatactaaag ggtgtacata taggaaataa tgtagttata ggtgcaaata gtttagtaaa
     1801 caaggacgta tcagacgatt gtgttgttgc aggaaatcta gcaaaagtaa ttatgtccct
     1861 agatgaatat tataaaaaga taaacaatat gaagaatccg taaaacttgt gaaactatac
     1921 cgagaataat atgggaaaaa tattgatgaa aaagcattat atgagttttt agggctgttt
     1981 acagatggag atgacaattt gtcgaagagt tgggagcaat gcaaaattta gtaggggatt
     2041 ataaacagac tgacaaggta tttaaagaac atgcacaagt atttcataat attgaagaat
     2101 ttttgaatac tgtgagatag gagttgctac attgagaaaa gtatgtattt ttccaatgta
     2161 aaagattacg agt
//
LOCUS       DAZKNM010000256         2150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000256 DAZKNM010000000
VERSION     DAZKNM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.264"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8M9_002745"
     CDS             complement(<1..914)
                     /locus_tag="ACG8M9_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0379440.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8M9_002746"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8M9_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0379441.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          728 a          380 c          414 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg
//
LOCUS       DAZKNM010000257         2117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000257 DAZKNM010000000
VERSION     DAZKNM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.265"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..266
                     /locus_tag="ACG8M9_002747"
     CDS             <1..266
                     /locus_tag="ACG8M9_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0379442.1"
                     /translation="EVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFF
                     FVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            263..982
                     /locus_tag="ACG8M9_002748"
     CDS             263..982
                     /locus_tag="ACG8M9_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0379443.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            997..2082
                     /locus_tag="ACG8M9_002749"
     CDS             997..2082
                     /locus_tag="ACG8M9_002749"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0379444.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT          771 a          229 c          428 g          689 t
ORIGIN      
        1 tagaagttgg aatattagga gtttcgatat tcctattttt tataataaag acatttttca
       61 atgcaaaaag atcttttatg aaggataaag atgcaagtct tgttaaagtt attgcaatta
      121 tgtcggtatt cttttttgtg tataacacaa cacttagggg tgaaacaacg gggccactga
      181 tttatatgat tttagccatg cctgaagttg tgaaaaggaa agagcattat tatagtgaaa
      241 aaaatacaaa aggagagata ttatgattcc taagaagata cattactgtt ggttcggggg
      301 aaatccactt ccgaaaaaag atagaaagtg catagaaagc tggaaaaagt attgccctga
      361 ttatgagatt attgaatgga atgaatcaaa ttatgattta aataagaacc gttatatggc
      421 cgatgcatat aaagaacgta aatggggttt tgttccagat tatgcaagat ttgatattat
      481 ttataatcat ggtggatttt atttagatac agatgttgag ctaattaagt cgttagatat
      541 acttcgtgaa aatcatgcct atatgggatt tgaaggggaa atctgggtta atggcggtat
      601 tggttttggt gcagaagaag gacattctgt tattaaaggt ttaagggata tttacgattc
      661 attaagtttt tatcaaaatg atggtacatt gaacttgaaa ccgagtccgt attatattac
      721 agattatctt gaatctattg gattggttcg cgataacagc gaacaaataa taaatggcct
      781 gcatatctat cctacggatt attttgctgc caaggattat gatactggaa aaatttataa
      841 aactagtaat acaataagca ttcatcaata taatgcatct tggaaaacac ctaaacaaaa
      901 atttgtttat agattaaaat caacaattgg acaaaaaaaa tttaacaggt tacttgcact
      961 gaaaaaattt ttacacttat aaaggagaaa agtactttga agaaaatttg tttcattagt
     1021 ggtgtaattt ctcgttctgg gggaactgaa agagtaggga gtattattgc caatagcttg
     1081 tctgatttag gttatgatgt cactatttta agcttttgga atagtggaga gccttatttt
     1141 gggttagata agacgattaa ggttgattat atgttaaatc ctaaaacgga gggaaaatta
     1201 tatagaactt atttatatcc ggtttggaaa ttgcacaagt atatatgcaa aaatgaattt
     1261 gatatagtaa ttgatattga tactttgttg tctttgtata ctacaagggc aattaaagga
     1321 acaaagtgca aattgatttc ttgggaacat tttaattact ggacaatgga aatgctgaaa
     1381 gaaaagaaga gattcaaggc taaaaaattg ataaaaaaat atgcaaagag gcttgtggtt
     1441 ctcacagaag aagatcgtca aaaacatatt gatgaatata gtttatcaga agattttgtt
     1501 ataactatgc caaatccgtg tatatctaat atcgagtacg aatatcatta tgataaaaag
     1561 agatttcttg cagtagggcg attagtggaa gagaaaaatt ttgctgacat gataagtgct
     1621 tggaaaattg tagaaaaaaa agtagatgat tgggatctta tcattgtagg aaaaggtgaa
     1681 ttagaaaaag atcttcgcca gcagataaaa gatttaaatc tgcaaagagt aactatagca
     1741 gggcattcag accatgtgga ggaatattat aaatctgcat cttgttatgt gcttagttct
     1801 gtttatgaag gatttcctat ggttatatta gaagcacagt cttatggatt accagttatt
     1861 tcttatgatt gtaaaactgg tccgagagat ttggtacatc ataattttaa tgggatgttg
     1921 gttgaagata agaatataga ccaattagct aagagcatga taatgtttac taagaatact
     1981 gatttagcaa tgagaatgtc actgaacgca tataataatg tacaaaaatt taatttgaaa
     2041 gaaattacaa aacaatgggt tgctttgatt gaaaatgtat agttttttgt ggttgtccaa
     2101 acatgaaaat tagcgta
//
LOCUS       DAZKNM010000258         2104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000258 DAZKNM010000000
VERSION     DAZKNM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.266"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            717..1049
                     /gene="traE"
                     /locus_tag="ACG8M9_002750"
     CDS             717..1049
                     /gene="traE"
                     /locus_tag="ACG8M9_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0379445.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1668..2009
                     /locus_tag="ACG8M9_002751"
     CDS             1668..2009
                     /locus_tag="ACG8M9_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0379446.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          740 a          278 c          437 g          649 t
ORIGIN      
        1 agaatttata agttaaaata ttatgataaa aatcacaaac gtagcctttt acgtcataag
       61 tgttaaaaga aataattaat ttcaaatata gaaaagggaa cgtatgtttg catactcctt
      121 ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac aagctaaaaa
      181 tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt tttgtataaa
      241 tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ttgcactttt ggatgataaa
      301 tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag gctggtaacc
      361 ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac ctttcagtgt
      421 tacggtatta cccgcaatgg atttagtata acagatattc aaaagtgata ccactttttt
      481 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcataatgt
      541 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
      601 tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc tctatttttg
      661 aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt tattttatgt
      721 catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta
      781 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
      841 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt gggaaacaaa
      901 agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca atcaaacatg
      961 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
     1021 caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg tattttatcg
     1081 tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt cactagaaaa
     1141 ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg taggttttaa
     1201 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa
     1261 atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa gttagacaag
     1321 ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact ttgacgcaaa
     1381 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
     1441 gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat cgagcattta
     1501 ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat tttttatgta
     1561 tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt gaaaaaatta
     1621 atataaagca tagagaaaat gaaatttacg taatggagga atgacaaatg aaaaattatg
     1681 atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt tgggattaca
     1741 aaggatttgt gacgtttaga aggggcggaa attgtaaagg attagatgtt ttagcattag
     1801 acgaggatga tttatacgat caaacattga ctgataatcc aattggattc ggcttgctac
     1861 ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat aatggagacg
     1921 aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc tttgaaatca
     1981 ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta cagataaagg
     2041 cttagaagga gtcacgacga ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
     2101 attt
//
LOCUS       DAZKNM010000259         2103 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000259 DAZKNM010000000
VERSION     DAZKNM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.267"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..445
                     /locus_tag="ACG8M9_002752"
     CDS             122..445
                     /locus_tag="ACG8M9_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379447.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            609..863
                     /locus_tag="ACG8M9_002753"
     CDS             609..863
                     /locus_tag="ACG8M9_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379448.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            933..1178
                     /locus_tag="ACG8M9_002754"
     CDS             933..1178
                     /locus_tag="ACG8M9_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379449.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1254..1619
                     /locus_tag="ACG8M9_002755"
     CDS             1254..1619
                     /locus_tag="ACG8M9_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0379450.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1680..>2103)
                     /locus_tag="ACG8M9_002756"
     CDS             complement(1680..>2103)
                     /locus_tag="ACG8M9_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379451.1"
                     /translation="LFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDY
                     RGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNH
                     VRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          588 a          363 c          358 g          794 t
ORIGIN      
        1 tcaaaatata agtaaaataa tattgttatt cttatttctt ttacttgtgt cataactatt
       61 ttgttacttt aatttttatt tgcttatttt agaaaaaatg ttattctttt atttattctc
      121 aatgttagga gtgattattt tgccatttat tgcgatttta ttcgatttag ttgcggcagc
      181 agcttatttt ctccaattaa attatcaaac ctcaggaatt ctgtttattg gaatgatttt
      241 tcaaggtgtg attacccttt tgcttcttgt catgatgctc agctataaag gaaaaagata
      301 tgcccgtatt caaacaaaag tatttgttaa atatgtttcc attcgctatg ggatcatcat
      361 tctttccttt ttagtgaatg cgatcgtcct ctttttatat gtattgaatt acttagatat
      421 caatccattg attttttctc gatagataag acacattcag cggaacagtt cctgcttatc
      481 agcagaaaac tgttctgctt tttcattttt cattgatcta ttcttctttt attttcctag
      541 catttcattt gtgatgtatg caataacatg ttatctttta ttatatgaat cgttgaggag
      601 tccgttacat gattacgttg gtgagagttt tgttttgggt gcctgcggtc gcactggtcg
      661 caagtattgt ttatcttatg aattggaaca aagaacggtt ttatttagct attttgactc
      721 ttccggctat ctactttatg tggaaagttt ttaattacaa ctactttgaa cccgatagtg
      781 tttttataga agaattatca ggactggttt tgtctttatt gatcgtcatt ctttatttga
      841 tacgtttaaa taaaaaacat tgagtactat tgattgcatg tgtagctttc tatagtcatt
      901 ataactattc tactataatg aaaggtgatt aaatgagtat cgaagaagct attttactgg
      961 atactttacg ttgtcgtctg aatttcttaa gaactggccg atctttatct caagtagctg
     1021 tttctagcgc gttaagtatt cctttattca aatatcgtaa gtacgaagaa ggagaagaaa
     1081 ttccaagcat cattgaatta atctatatcg ctgactttta cgatatttcc gttgattacc
     1141 taattggtcg ctcagaaaca gcaagcaaac tgaagtgatt ttttccagcc ttggtgctgg
     1201 ttttttcatt taacgtctaa ggttaacaat ctatatataa aggagacatt tctatgcgat
     1261 tatggcatga aaaattgatt tcaaagcttc ctcgtcaaca gcttttagga caacatcggg
     1321 aatgctgtgc attaagagga aatggctggg gaaagaaaca tgctactgtg gattatgttt
     1381 ttcactattc tccttataga ttattccaat atcatcagct tgtcatggat gaaatggaaa
     1441 aacgtggtta cagcccagca ccagagtgga aaaatcctat gtatcgtggc aaatcatgcg
     1501 aagcattttt tgatttacct actgtagagc gcaacgtgcc gatttatccg gaacacgatg
     1561 aagtttatct agcagaatgt ttagcaaatt tggagcaaaa aggaatttat cttacctaat
     1621 ataactagaa aagccttcta tctcctctta cttcattaaa aagctttaac catcttagat
     1681 caagctaaaa tgtcgtaagt tttagggtta tcttcatcct cacgtgcaaa actaaaagcg
     1741 tactctagtt ttggttcatc gccaacttcc ctagacataa atgaacatct cctgacgtgg
     1801 tttaaaagta ccgcatcttc gacgtctgta tacgatgcac agcacagatt gcttcttttg
     1861 atctttcttt tgatttttct gttgcttctt ctacaccttc ttcgattact acatcgatgc
     1921 ttacaattgc ttctgcctaa tattcccatg catcgtcgat ttaagcctct gtagtcactg
     1981 tgatcgtttc cacattttaa ctcctctgtc gtggcttcag ttgattttgt gatcagttca
     2041 gttgctaagc cagagtcaga acaacctgcc attgctagcg ttaatattga tagtaaaaat
     2101 agt
//
LOCUS       DAZKNM010000260         2053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000260 DAZKNM010000000
VERSION     DAZKNM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.268"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..353)
                     /locus_tag="ACG8M9_002757"
     CDS             complement(27..353)
                     /locus_tag="ACG8M9_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379452.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(399..761)
                     /locus_tag="ACG8M9_002758"
     CDS             complement(399..761)
                     /locus_tag="ACG8M9_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0379453.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(798..1012)
                     /locus_tag="ACG8M9_002759"
                     /pseudo
     CDS             complement(798..1012)
                     /locus_tag="ACG8M9_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1140..1475
                     /locus_tag="ACG8M9_002760"
     CDS             1140..1475
                     /locus_tag="ACG8M9_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379454.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1551..>2053)
                     /locus_tag="ACG8M9_002761"
     CDS             complement(1551..>2053)
                     /locus_tag="ACG8M9_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379455.1"
                     /translation="TYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHE
                     VLQGLSWQGTYLMTEKELTNTVCERLRNVGNNSVENALKENRGYFVKQGFTLERASKD
                     IKRLYNVKASSSFLVYLAKGAGIDEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRK
                     DPIVLN"
BASE COUNT          628 a          423 c          287 g          715 t
ORIGIN      
        1 tggacggtat gcataataca tttaaatcaa tttaacatta acacctcagc atatggtatc
       61 tccacatata tacggtcttt ctttggatag tactcaatat ttatgttgat agcacttcca
      121 ttgtctaata tagctttagc ccggtatgtg ttatctccat tttcatgagt aatcgtcaca
      181 tcatcacatg atcgatttaa gttgtactgt tcttgtaaaa tctgtgttac caatggtttc
      241 gatgacttct ctaatccaga tgtacctgtc atttttgaaa aaatgatacc tataatagcc
      301 aagacaacga taactataat gataaacttc ttgttcatat ttactttatt caacttttta
      361 tctcctatct ccaaaataat ttgctcttat ccctcttatc agtacattga catccctaaa
      421 agtacaccaa agattgctcc agctacccct gctatcatca aaatcgtacc catttgttca
      481 tctttatctc gatataagta gcccataata acagcaacag caccaaatac aacaggaata
      541 aatgctaacg ctactaccat agaaatccaa ccaataatca aatacagctt ccctgtctct
      601 tgtccatgta cgatttgttc cttcatttca ttaattgtct gctcagtact tttctttact
      661 gcgtctgctc cagttgtctc atctgtttct tgtagtgctc cgcaaaactg gcaatactta
      721 ctttcatctg ttatttcttt gccacatttt ctacaataca tgtcttctcc tcctaaaaac
      781 attcaattgt ttattagtta actgtttact ttattaaata catcagaaaa cagctcattc
      841 attggtacac ttaatatctc agaaagcatc ttcatttgat agggtttagg aatcgttctc
      901 cctgtttgcc acgaacttac acattgctgt gtaacattca tttttttgct aactccgttt
      961 gagaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc
     1021 cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat ataaaaaaac
     1081 ttcaaatacc attacaaata caacaacttg ttgtactata ctgagatggg ggtgtattaa
     1141 tgaaacagaa acaatatgca aaggtcatta gagaaaagcg aaaagctctc gggtggacac
     1201 aaaaagaatt agctgaaaaa atcttctcta cacaacaggc agtagcaaga tgggaaaatt
     1261 ctgtaacaga gcctaacctc gatagcttaa ccgctctttc tagagcccta ggaacccctg
     1321 taagccattt tctagacaat gttgtcgtag attatgagga agagtttttg gctctctatc
     1381 gttctcttag cactgaagat gcagttcgaa caattgacta tatgaagcta ttaaaaaggc
     1441 aagaaaatga acgcaatcag ctattgaaag attgaaaaat aaataaccca ccgaacttta
     1501 acggtgggtg tattgagatg aaataagatt aagagctatg gataacctgt ctagtttaat
     1561 acaattgggt cttttctctt ccttactctc cgtccatcat ctcttgttac acttgtaaat
     1621 tgataaagag gaactaattc aactcctgat aatatcaatt cttttattac ttcatctatc
     1681 cctgctcctt ttgctaggta gactaaaaaa ctactacttg ctttcacatt gtatagtctc
     1741 ttaatatcct tagaagctcg ttcaagagtg aatccttgtt taacaaagta gcctctgttt
     1801 tcttttaaag cattttccac gctgttattc ccaacattac gtagacgttc acaaacagtg
     1861 ttagtaagtt ctttctctgt cattagatat gttccttgcc acgataagcc ctgtaaaact
     1921 tcatgtgctt catctaaaat caattgtttt tccttgctaa gttctgatcg ggaattttga
     1981 ggaaatactc ttttagcctc tttttcgtta aaacatatct taacccattc ataagaaaaa
     2041 tattgatatg taa
//
LOCUS       DAZKNM010000261         2008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000261 DAZKNM010000000
VERSION     DAZKNM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.269"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8M9_002762"
     CDS             200..742
                     /locus_tag="ACG8M9_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379456.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ACG8M9_002763"
     CDS             898..1347
                     /locus_tag="ACG8M9_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0379457.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2008)
                     /locus_tag="ACG8M9_002764"
     CDS             complement(1377..>2008)
                     /locus_tag="ACG8M9_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0379458.1"
                     /translation="LTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLT
                     YVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRG
                     KEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRL
                     ELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          729 a          312 c          381 g          586 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatc
//
LOCUS       DAZKNM010000262         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000262 DAZKNM010000000
VERSION     DAZKNM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.270"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8M9_002765"
     CDS             <1..378
                     /locus_tag="ACG8M9_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0379459.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8M9_002766"
     CDS             426..1826
                     /locus_tag="ACG8M9_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0379460.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKNM010000263         1997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000263 DAZKNM010000000
VERSION     DAZKNM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.271"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..303
                     /locus_tag="ACG8M9_002767"
     CDS             <1..303
                     /locus_tag="ACG8M9_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0379461.1"
                     /translation="MIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENE
                     IETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            276..953
                     /locus_tag="ACG8M9_002768"
     CDS             276..953
                     /locus_tag="ACG8M9_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0379462.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            904..>1997
                     /locus_tag="ACG8M9_002769"
     CDS             904..>1997
                     /locus_tag="ACG8M9_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0379463.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLF"
BASE COUNT          739 a          269 c          401 g          588 t
ORIGIN      
        1 atgatcggaa attcttcttc tgggataatt gaagctgcat cattcaaaat accagtaata
       61 aatttaggaa ccagacaaaa tggacgagaa caatctaaaa atattataaa tgcaaatcta
      121 aatgaaaatg aaattgaaac tgctttgaat aaaatacaag atcctcagtt tataaaaagc
      181 ttgaaaaatg ttaagaacat ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca
      241 ttgaatcaaa tattgaatga tggggaagat attaagtgga ttcaaaaaca aatagcttat
      301 taaaatatac tagtgatcca tacattatta ttggggctgg cggacacgca aaagtcatta
      361 ttgaaacctt aagattagaa aataagttaa tatatggatt ttttgatgat aagcaaaata
      421 aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt gaatagctgg gcggatagca
      481 aaaaaaaaat ctataaattt attgtagcta ttggtgactg tgtagtaaga aaaaagatta
      541 taaaaaaatt gaatatgcca agtgagcaat atggaatagc tgttcatcca agtgctatag
      601 tggcaaaaga aatttcaatt ggttatggaa ctgttatttt tgcgaatgct gtaattaacc
      661 cagatgctgt aattggagag catgctataa taaacacagg tagcattgtt gagcatgatt
      721 gtagaattaa taactatgtt catttatctc caggtgtatg cttagcgggt ggagtacatg
      781 taggtgtagg aactcaaata ggaattggtt ctcaatgtat ccagatgaag aaaattggat
      841 cttggtgtat gataggcgca gggagcacta ttgttaagga tattccctca cattccttag
      901 cttatggcaa tccagctaaa attaagaaag aaggaattaa ttttgaattc tagaatttta
      961 ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat
     1021 aattggatag cgccattagg tgaaaatgtg gataaatttg aagaaagtat tacaaaatat
     1081 actggttcta agggcggagc ttctgcgtta agttcaggta cagctgccat acatcttgcc
     1141 ttaagactgc ttaatgttgg aaaaaacgat attgtctttg ctccttcatt tacttttata
     1201 gctacagtaa atcctatttt atatcaaggg gctgtaccag tgttcattga ctcagaacct
     1261 aatacatgga atatgagccc tgttgcacta gagagcgcat tagaagaagc tgaaagaaac
     1321 aaccaattac ctaaggcaat catcgtagtg aatttatatg gtcaaatggc tgattatgaa
     1381 cgaattttag ctattgcaaa ccaatttaat gttccagtga tagaagatgc agcggaatcg
     1441 ttaggttcta cttataaggg aatttcgtca ggcttatttg ggacaattgg gatctattca
     1501 tttaacggta ataagatcat cacaacctca ggtggaggag ctctagtgtc tagtgataaa
     1561 aaatatgttg agcattcaag atttttagca acacaagcaa gagatcaagc accgtattat
     1621 caacatagcg aaataggtta taattaccgt ctaagcaata tttctgccgg tatcggacgt
     1681 ggacaaatgg aagtattgaa tgatagagta gctaaacgaa gagaaatttt caatcgatat
     1741 aaggaagctt tttctggaat tgatagaatt gaaatgatgc cagaagtgga tggcatggtg
     1801 ccaaatcgtt ggttgtctac tttaacgatc aatgattata attctattga tcaagtagtt
     1861 aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg gaaaccacta
     1921 catttgcaac cagtatttca aggggtaaaa atgtattacc atgataataa tcttagtgtg
     1981 tcagaaaagt tatttaa
//
LOCUS       DAZKNM010000264         1985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000264 DAZKNM010000000
VERSION     DAZKNM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.272"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..410
                     /locus_tag="ACG8M9_002770"
     CDS             174..410
                     /locus_tag="ACG8M9_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0379464.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            429..644
                     /locus_tag="ACG8M9_002771"
     CDS             429..644
                     /locus_tag="ACG8M9_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0379465.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1347..>1985)
                     /locus_tag="ACG8M9_002772"
     CDS             complement(1347..>1985)
                     /locus_tag="ACG8M9_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0379466.1"
                     /translation="TQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYD
                     VQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQ
                     VTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPM
                     LTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT          647 a          309 c          351 g          678 t
ORIGIN      
        1 tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg aatcaaattt
       61 attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga tttttactgt
      121 taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga agcgtgttta
      181 aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt ggtggaggca
      241 ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg gcagtaggtg
      301 gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga gggttatatg
      361 gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa aaaaggagaa
      421 taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca gaagagttga
      481 aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct ggggcaacag
      541 caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt ggtgtaggtg
      601 gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt aaaaaatatc
      661 aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat cttttcaaaa
      721 gtttggaatg gacagaactt tttactaaag cttcttgtgc tagtttttat aattatatta
      781 aatttttatc ttttattaaa gtcagataaa aattctttgg aaatgtaatg gtagagatat
      841 ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag gaggacctat ttcggccgtt
      901 acaggaggta ttggtaatgt acttgtcgga tgtgcattaa agcctgttcc tgcaagttaa
      961 atttataaaa acaggaaagt aattatctca aatcctatca agtacaatca taggattgca
     1021 cttgacagga caaatttttt aactaaaaaa tgaaaaacac tttctaaaaa ttagtagaat
     1081 ttgccaaatc tttcgcatct tccaccatac atttaatttt aattcccctt tatggtactt
     1141 aataaaaaca agaaaagaac tattcatttg gccctttttt ggttccagac catagaaata
     1201 ttcctccagt aactaatatt gctgttatgc acgtaactaa taacagctat aaaaattggt
     1261 acaactttaa taggattcag aaaaatttgc actagtgaag gaatctattt tattagaaaa
     1321 atgcaatctc cctaaagaaa aaaccactaa acagtgcttt ctttcaactt ctccttaatt
     1381 ttttgtgctt tccctaaagt aaagtttctg gccaactctg aatcaaaata tagtgaacgt
     1441 tttttaaaat ctatttcttt aacgttaaag atatttacta aacacgctcg actaattctt
     1501 gttaacatag gatatttctt ttcaagctca ctaattcttc cataaaattc gtactgccca
     1561 tctttagtac acaatgataa tctatgtggt aattttgaag cttctacaaa ataaatgtca
     1621 tttatatcaa aagtaaatgt ttgagaacct atggaaaaaa caaatgcttg attcgtaacc
     1681 tgttttgttg catctatcct ttcttgcgct aataacaata actcaacaat ctcgtttcga
     1741 tactcatcta gtgtttgatc ttttgttaca aatcctaacg tttcaactct tctttttatt
     1801 gttacgggta acatctcatc atgagtagtt gtaaaaatga tttttgcttg aacatcatat
     1861 tttctgatta cttctgctaa ttctataccg ttaacttcat gatttaaatc aatatctaag
     1921 aaatatattc cttgctttgg tcggaattgt ttgaggtatt ttttaacttc taacggactt
     1981 tgtgt
//
LOCUS       DAZKNM010000265         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000265 DAZKNM010000000
VERSION     DAZKNM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.273"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8M9_002773"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8M9_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8M9_002774"
     CDS             complement(728..1744)
                     /locus_tag="ACG8M9_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0379467.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKNM010000266         1974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000266 DAZKNM010000000
VERSION     DAZKNM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.274"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..550
                     /locus_tag="ACG8M9_002775"
     CDS             <1..550
                     /locus_tag="ACG8M9_002775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0379468.1"
                     /translation="VSLTKELSNPSLKREIEYSQSEDLLKALKWSEEVNQQISQAKGL
                     DKPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERHELLSLVDELCCQDKGKKEDI
                     IRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVNKEKESWENLRTDVLFKCL
                     SGDYVDIEQKYIDTFWKNLTNK"
     gene            563..>1974
                     /locus_tag="ACG8M9_002776"
     CDS             563..>1974
                     /locus_tag="ACG8M9_002776"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFQ0379469.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKI"
BASE COUNT          734 a          228 c          403 g          609 t
ORIGIN      
        1 ggtatctcta acaaaagaat tgtctaatcc atctttgaag agggaaatag aatattctca
       61 gtcagaggat ttattaaaag ctttaaaatg gtcggaagaa gtcaatcaac agattagtca
      121 agcaaaagga ttagataaac cctttttaag tgctagagat actataaaag ctctgaaaaa
      181 atatgggaag ataattattg taagctcagc taataaggaa gctgttcaag aagaatggga
      241 aagacatgaa ttattatcat tagttgatga actgtgttgt caagataaag gaaaaaaaga
      301 agatattatt cgaagtgtaa ttgaaaatgg ttgtgatttg gataagatat taatgattgg
      361 tgattctcct ggtgatctag aagctgcaaa taaaaataaa gttttctttt atcctatttt
      421 ggtcaataaa gaaaaagaat catgggaaaa cttaagaact gatgttttat ttaaatgttt
      481 atcgggagat tatgtagata ttgaacaaaa atatatagat actttttgga aaaatcttac
      541 taataagtag aggagaaaga aaatgactat aaaagtagat ttacgaaaaa aaccatataa
      601 tttagatgaa gatgctattt cttggattga agagacgatt gattctatga ctttggaaga
      661 aaaaataggt caattgtttg ttaatatggg atctagtcgt gataaaaatt acttgagtaa
      721 tatggtcaat cagtaccata ttggagctgt acgatataat ccaggaactg ctgatgaggt
      781 atatgatcaa aactatatct tacaaacaga aagtaaaatt cctctattaa ttgctgctaa
      841 tacggaagca ggtggtaacg gagcttgtac agatggtact gaagttggtg tagaagtaaa
      901 aattggtgca acaaatgatg cgaaatatgc ttatgaatta gggagagttt ctgggattga
      961 agcatcggct attggatgta actggagttt tgctcctatt gtagatatta atagaaattg
     1021 gagaaatcct atcatttcta gtcgatcttt tggacaaaac cctgataaag ttttagaact
     1081 tagtttagct tatatgaaag gtattcagga gagtggaatt ttgcctgcag ctaaacattt
     1141 ccctggtgac ggtattgacg aacgagatca gcatctatct tttagtatta atagtttagg
     1201 tacggaagaa tgggataatt cttttggaaa agtatactca ggcttaatag aagctggatt
     1261 accttcaatt atggcaggtc atatccatat gccagcttat tcaaaacatt ttaatcctga
     1321 tctgaaagat gaagatttac ttcctgcaac tttatcaaaa gaattaatca ctgacttact
     1381 tagaggaaaa ctcggattta atggattagt agttactgat gctagtcata tgttaggaat
     1441 gactggggca atgaaacgat cagaattatt gcccacttct attgcagctg gatgtgatct
     1501 atttctattt tttaatgatc cagatgaaga ctttaattat atgatggaag gctatcgtaa
     1561 aggaattatc actgatgatc ggttattaga tgccttaaaa agaattttag ggttaaaagc
     1621 taaattggga ttacacaaaa aagcaaaaga acaaatttta ttacctaagc aagaagctct
     1681 ggaaaaaatt ggtttaattg aaaataaatc aatttttaaa gaggtggctg accaagcaat
     1741 tacattagtt aaagataaac aagatatttg gcctattgta ccagaaaaat ataaaaggat
     1801 tttactagta gatgtacatg gggtaaaagg tgggtttggt gaagtagttg ctggaaatag
     1861 acctcaagcc attgatttat tgaaacagat tttgtcagca aaaggatttg aagttgaaaa
     1921 atacatttca cctgaagaag aattgaaagg attatcgcaa gaagaaaaaa ttaa
//
LOCUS       DAZKNM010000267         1941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000267 DAZKNM010000000
VERSION     DAZKNM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.275"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="ACG8M9_002777"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="ACG8M9_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0379470.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="ACG8M9_002778"
     CDS             851..1039
                     /locus_tag="ACG8M9_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0379471.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="ACG8M9_002779"
     CDS             1052..1609
                     /locus_tag="ACG8M9_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0379472.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="ACG8M9_002780"
     CDS             1641..1883
                     /locus_tag="ACG8M9_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0379473.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          714 a          300 c          400 g          527 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac a
//
LOCUS       DAZKNM010000268         1926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000268 DAZKNM010000000
VERSION     DAZKNM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.276"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            468..1802
                     /gene="dnaA"
                     /locus_tag="ACG8M9_002781"
     CDS             468..1802
                     /gene="dnaA"
                     /locus_tag="ACG8M9_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0379474.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT          639 a          344 c          378 g          565 t
ORIGIN      
        1 agtataacga caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt
       61 atccacaaga ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt
      121 atccacagat aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc
      181 acaatcaggc gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta
      241 tccacagacg caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg
      301 tgtggaaaac tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg
      361 ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc
      421 taaattagta ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg
      481 atgctttatg gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata
      541 atacatggat cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg
      601 ttcctagcaa gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag
      661 tcggttatat gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag
      721 agcaagaaga agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa
      781 aagccatgct gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga
      841 tggctcatgc agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct
      901 tcttttatgg aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa
      961 tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg
     1021 atttcatcaa ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg
     1081 ttgatttact gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag
     1141 agtttttcca tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg
     1201 atcggttgcc aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg
     1261 ggctgtctgt tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa
     1321 aagcggcggc tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa
     1381 ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga
     1441 tgaatagtga agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag
     1501 gaaaaggaca tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc
     1561 acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac
     1621 aaatcgctat gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg
     1681 aattcggtgg caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga
     1741 tggattctag tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt
     1801 aatttgtcga ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag
     1861 ttatacacac tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc
     1921 acagaa
//
LOCUS       DAZKNM010000269         1916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000269 DAZKNM010000000
VERSION     DAZKNM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.277"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1295
                     /gene="tgt"
                     /locus_tag="ACG8M9_002782"
     CDS             150..1295
                     /gene="tgt"
                     /locus_tag="ACG8M9_002782"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0379475.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1392..1772
                     /gene="yajC"
                     /locus_tag="ACG8M9_002783"
     CDS             1392..1772
                     /gene="yajC"
                     /locus_tag="ACG8M9_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0379476.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
BASE COUNT          663 a          315 c          416 g          522 t
ORIGIN      
        1 ttttcttttt tttgttattc ataaaaaaag gataaaaaaa agaatattaa ataaagtaga
       61 aggaagccga gggaacttgc aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa
      121 gatattttat atagaaagta gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa
      181 tcaagaaaga aaaacatact ggcgcgcgtt taggggaatt gatcacgcca cacggaactt
      241 tccccacacc aatgttcatg ccagtaggaa cattagctac agtaaaaaca atgtccccag
      301 aagagttgaa agaaatgggt gctggcgtta ttttaagtaa tacatatcat ttatggctac
      361 gccctggtga agacttagta gaagaagctg gcggactgca taaatttatg aactgggatc
      421 aaccgatttt aacagattca ggcggctttc aagttttttc attaagtgat atgcggaata
      481 tcgaagaaga aggcgtgcat ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac
      541 cagaaaaagc aatcaacatc caaaataaat tgggctctga tatcatgatg agttttgacg
      601 aatgtccacc attcgatgaa agttacgact acgtgaaaaa atcagtagaa cgtacgtctc
      661 gatgggcaga acgaggattg aaagctcatg ctaatccaga ccgtcaagga ttatttggaa
      721 tcatccaagg agcagggttt gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga
      781 tggatttccc tggttactca atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga
      841 atcgtgtatt agaattcact acaccgttga tccccgaaaa caaaccacgt tacttgatgg
      901 gtgtcggtgc tgctgattca ttgatcgatg gtgttatccg tggagtagac atgtttgact
      961 gtgtcttgcc tactcgaatc gctcgaaatg gcacatgtat gacttctcaa ggccgtctag
     1021 tagtgaaaaa tgcgaaatat gcccatgatt tccgtccaat tgatgagaaa tgtgattgtt
     1081 atacatgtaa aaactataca cgtgcctata tccgccattt gatcaaatgt gatgaaacat
     1141 ttgggattcg tttgacttct taccacaatt tatacttctt gttgaatcta atgaaacaag
     1201 tacgtcaagc aatcatggat gataatttgc ttgaatttag agaagccttt tttgaagaat
     1261 acgggttcaa taaagaaaac gcaaaaagct tctaaaggaa aatcttttaa aaattaagta
     1321 aaaaactagt gttatcagca tttttctgct aaagtgtttt ttgtagatac agaattagtg
     1381 aggtgaaaaa aatgggaaca ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt
     1441 ggttttttat gtcacgttcg caaaagaaac aacagcaaga acgtcaaaac ttgttagata
     1501 gcatgaaacc aggtgattca gtcgtaacga tcggcggatt acatggagtt gtttcagaaa
     1561 tcgacaatga caaacggaca gttgttttag actgcgaagg gatcttcctt gagtacgatc
     1621 gtgcagcaat caaaacggtc aaaccaggga ctgtcgtaac aaatgatgca acgactactg
     1681 ttgaaacaac agaagaacca gctgagacag ttgaagtaaa aactgaaaca acagaaacgc
     1741 tggaagagac agaagagaag gaagaaaaat aagtatcaaa gcttatttaa agacgaggta
     1801 gggcagaaag atgtcctacc tcatttttta ttcaaaaaag aaagagataa gagcatggga
     1861 tatattgctc aatattgaat aatcgggtgt gtcatataaa taataataaa taactt
//
LOCUS       DAZKNM010000270         1886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000270 DAZKNM010000000
VERSION     DAZKNM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.278"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..736)
                     /locus_tag="ACG8M9_002784"
     CDS             complement(35..736)
                     /locus_tag="ACG8M9_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0379477.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(752..1192)
                     /locus_tag="ACG8M9_002785"
     CDS             complement(752..1192)
                     /locus_tag="ACG8M9_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0379478.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1192..1767)
                     /locus_tag="ACG8M9_002786"
     CDS             complement(1192..1767)
                     /locus_tag="ACG8M9_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0379479.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          531 a          404 c          293 g          658 t
ORIGIN      
        1 taatttttct ttcccgtcgt ttggtttttt atttttagcc taattcttga ccttttgcat
       61 gctggttatc cgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt
      121 ggatctgacg gatcaccttt tttgcatctt ctggattttc tacaaaatcg aatttatccc
      181 catccacttg tattttagga catacatcaa aatcttcata ccattgatta tagcgggtgt
      241 tcaacgtttc ataataggta tataacgtct catcgctttc tagctgttca tagtcacgcc
      301 cacgtttttt gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat
      361 caggccgttt ttttggagca acatcatcta actcgtttag catcgtatct aataagctat
      421 cgtactgctg tacttcgata tccgttaccc gacctaaatc agcgtttaga tggaacaaca
      481 ggctatcttc atagatggat cggtctagca cattgtcatc gttcactaaa gcatttttca
      541 ttgctaaaaa tcgcttgttc aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat
      601 ttgaataaaa gagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat
      661 caatctcttt tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa
      721 tcaaaatcac tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa
      781 caggcaatgt ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtatgga
      841 gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt
      901 ttttcaacat agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct
      961 caaagtcaag gatagcaaca acaaattctg catttgtcag ttcttccata tctttttcgt
     1021 atacttcctg cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg
     1081 gagagtagaa acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc
     1141 gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc
     1201 ctccttcaat aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa
     1261 tggattgttg gaaaagatta cttgatccaa ccatttttta tccataaaat cctcttttgc
     1321 aaaagtttct aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat
     1381 tggcttgtcg ggcgtcagac cgattgctat ccaagaaaga agttgtgaga attcctccaa
     1441 tgtacctaga cctccaggga aaacgaggac agcatctccc ttttcaaata ataatacctt
     1501 tctttcatcg atcgtttctg taggaataaa tgtagtaaca tcttttccag gcaattcagt
     1561 ttcaaaaagc ccattcggat agatacctgt gacttttccg ttattttcca gtactgtttt
     1621 actaatcact cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg
     1681 tgccatgtat tttcctaacg tttgcgcaat aaatttgtaa ctttctttat taccaaaacg
     1741 ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt
     1801 cattctacac gtgttttttt aaaataaaag actagatttt gtagtttttt agtggtatat
     1861 aggatagacg ccactatata tagtat
//
LOCUS       DAZKNM010000271         1857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000271 DAZKNM010000000
VERSION     DAZKNM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.279"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..1655
                     /locus_tag="ACG8M9_002787"
     CDS             177..1655
                     /locus_tag="ACG8M9_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379480.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          688 a          255 c          315 g          599 t
ORIGIN      
        1 gaatatttcc tcataaattt ttccattttt tgcaatattc tcaaaaaaaa gataaaaaat
       61 gagaataact gatgaaaaaa caagcataat ccatcataaa atcagtatag gtgaaaagat
      121 gaactttcat ctgcatatca agtaattggt atgaggcatt ttttgaggtg taggatatga
      181 agttggatta ccttatagaa gaggaagata gagaaaaact gctgctagct agatatttag
      241 aatttgagaa aaagaattat gtagtgctgc ctaagttata tgaaacatta ggtttgtcta
      301 aatttaaaac aagaaattat atcatagaat taaataaaga atttactgaa ttcaaaacaa
      361 agccagagat ccaaatttca gaagaagcag agatccatgt cgaacgtctg aatttgacgg
      421 atatcaaaaa tttaagaaga atctactttc aacgatcaaa aattgctcga ctgttaacgg
      481 agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga gaatcatttt ttaagcatat
      541 caagagccta tgtaaaaaga agagaattag tctcttttct tgaacagcat caaataaagt
      601 taaagaaaaa catattagtt ggggaagaaa taaacatacg gaacgtatta tttggggtat
      661 atttcggggt ctttaatggt ttttctatgc catttagaga aagcgtcatg aatgaagcaa
      721 agagtatcgt gcaatatttt gaatatctgt tcaatttaaa actgtctgca acaaaacgaa
      781 tcaaattcaa tttgctaacc actattagtt tgttgagggc ctatgggaat caaccaatcg
      841 gaaaacgttt cttttcagat gaatacttta agagctcaga aggaatgaag atcatcaagg
      901 atttatcaca tatttctttt cttcatatcc ctaaagagca gatcattgat gagatttctt
      961 atatcatgtt gtttttgatc acggaagaag aatttgaatt agatggcgca tttggacttg
     1021 cggaagctca ttttcatgat atccacatga ttagtcaaaa aatgtcagtg gaattgattg
     1081 atcaattacc attttcgatt gaaatctctg aagaagaaag actatatata cgaaaagaac
     1141 ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat attcgacttt gagatgacta
     1201 gctttacgtc taaaaagcag ctacagtttt tcttcgagac gtatccaata ttcagtacag
     1261 caatccaaaa ggtcatacaa gagaataaac gctatttctt gggaaaagat gaacgtttca
     1321 tgattcgctt attttatgat tatctctttt tattggtcgg tatttgtccg gtatcatatt
     1381 ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg atctgcttat accgattaca
     1441 ttatttctca agtgaaagga tttaaaaatt acaatattgt gattgagagt cgaatcactt
     1501 ctcgtacgaa tatttattta tctgattgtt tacttgaaaa atttaataaa gaacagataa
     1561 tttggaaaaa tccaccgact ccagatgact gggaatattt tggaaatacc atcatcagag
     1621 aaaaaaatag acatttaact gaaaaaatca gctagtattt atggatggca gctttgtctc
     1681 acatatttat tagctgattt atgttttttt caaagaaaag gtaactagat ataactagat
     1741 ataactagat atattactta acaaaattaa aaagtacata agttattatt ccgattttct
     1801 tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt ccatcttctt ttgattt
//
LOCUS       DAZKNM010000272         1855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000272 DAZKNM010000000
VERSION     DAZKNM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.280"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..561)
                     /locus_tag="ACG8M9_002788"
     CDS             complement(52..561)
                     /locus_tag="ACG8M9_002788"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0379481.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(673..1671)
                     /locus_tag="ACG8M9_002789"
     CDS             complement(673..1671)
                     /locus_tag="ACG8M9_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0379482.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT          562 a          352 c          332 g          609 t
ORIGIN      
        1 aaaaaagaaa gaactcgtct aaatagacgt tttctttctt tttttgttga attattgagt
       61 aaggcgactt cttttttctg gttctttgtg tgctaatcga ctggcagcag cagcggcgat
      121 cgctccgacg atatcatcta agaaggtatg gacatgaacg ccatcgtggc tgtttagttc
      181 ttttaaaata ccaggtttca ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc
      241 gtaaacattc acgattgaca aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc
      301 ttcgataata tgttgcaacg gactcatcaa ttttttcttt tctgctaata tatctaattg
      361 gattccagtg ataatggcgt tatgtacttc acgctttgtt agcacgctgt tgacactttc
      421 agtacaatcc gccaaagtca aatcagaaat atagtctttt tgtaaaaaca tgacaagttg
      481 tgcgatatct tctacagtta cgccacgttc cttcaatagt tctcttgcct tttcttctaa
      541 agtctcagtt ttgacgacca tattattgct ccccctttat tttttctttc tctatttcat
      601 gttaacggaa catgaaaaaa atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag
      661 agatataaaa aatcaactag gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg
      721 ctgagttcgt tcctttggat taatatagtg taaagcgttg ttcactgcag taggtgcttc
      781 accgagccca gtagcaatca gtttcacttt tccttcatag gtacagatgt caccagctgc
      841 ataaacgcct ggaatagaag tagacatatc tgattttaca gtaatggcat ttctggtact
      901 atcaagtccc caagaagaaa gatgttctaa attagaagag aatccataat tgacgatcag
      961 agaatcaatc atcaaatcaa tcgtctcgtc acttttcact ttttttaacc ggatatctgt
     1021 cagttcccca ttattgcctg ataatccatc gatgagatag ggagtaagca agttgacaga
     1081 tgaagatttc aagcgtgata cactatgttc gtgcgcacga aattccgggc gacgatgaat
     1141 caagtaaact tcgcttgcaa taggctctaa catcaacgtc caatcgatag cagaatcacc
     1201 acctccagca atagcgactt tttttccagc atacttcatc aaatcattca cgaaataatc
     1261 caatccgtgg ttttcaaatg attcagcatt atctaaattc aattttcttg gctggaatga
     1321 accatttcct aaagctaaaa tgactgcttt ggaataatgg atgcctttat ttgtagtaat
     1381 ctcgataact tcgtcttcac gagtaagcga gagaacttct tcttttaaat ggaatgtatg
     1441 gttaaaagta gttaattgtt tttctaattg atcgattaat tcacttgctt ttattgctgg
     1501 atacccagga atatcgtaaa tatatttttc gggatacaaa gttgctaatt gaccaccaag
     1561 ttgaggtaga ctgtcaatga tttttgtttt agcttggcgc attcctgcgt aaaaagcggc
     1621 aaacatccct acaggacctg caccgatgat tgtaatgtca tagatttcca taaaaattcc
     1681 tcccttttca cactatagca gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg
     1741 aaatcgaaac ggtaagttat agaatgtatc tagtgatgaa aaataaaaga aaacaaatct
     1801 agtattttta cgagaggtta ggtattatgt tattattatt atgaagtatg caaat
//
LOCUS       DAZKNM010000273         1831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000273 DAZKNM010000000
VERSION     DAZKNM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.281"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..1356)
                     /locus_tag="ACG8M9_002790"
     CDS             complement(25..1356)
                     /locus_tag="ACG8M9_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0379483.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1505..>1831)
                     /locus_tag="ACG8M9_002791"
     CDS             complement(1505..>1831)
                     /locus_tag="ACG8M9_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0379484.1"
                     /translation="RFIDCQPYSIEQTFMPLAVIPGLEPKHLKKSVYAYITNELGLEI
                     KTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEYSVSRHLYQDFVFE
                     AVFVEK"
BASE COUNT          632 a          330 c          269 g          600 t
ORIGIN      
        1 taatttttgt tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg
       61 ttcgtcaatt cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg
      121 taaaacagct gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt
      181 agtccaagga atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc
      241 tactgtcaaa gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac
      301 aataggcaat ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa
      361 tttagcaaag tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc
      421 aatcgtattt cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat
      481 atgtggaatt tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa
      541 aaacggatct aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag
      601 attagcaata gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg
      661 ctctgtaact atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat
      721 aaatgacaat aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga
      781 ctttaaaaca gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga
      841 aagtttaata aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga
      901 agctcctgta taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc
      961 aggagtaaac atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac
     1021 cgctgcaata tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac
     1081 aactaataat gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa
     1141 cgtggtcaat gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac
     1201 tgaattaata agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc
     1261 atctcgcaaa gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt
     1321 ttcaaataca atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg
     1381 taatcgatta ctttttccac ccaaataata catcacttat caaaaaaagt aaatactttt
     1441 aattaaatat atatatcttt tttagtataa aaaaataagc tctgaaaaaa taattttccc
     1501 gagcttattt ttcaacaaaa acagcttcaa aaacaaagtc ttgatataaa tgtctcgata
     1561 ccgaatactc gaaaggttta ccagacgata gagcaacaac tttgtctatc tcaatcataa
     1621 attcacctgg atctattttt aatatttttg cgtcaaattc attcgcagta tctcctttga
     1681 tacgtatgtg cgaagtcttt atttccagac ccagttcatt tgtgatataa gcatatacag
     1741 atttctttaa atgttttggt tccaagcctg gtatgactgc taatggcatg aatgtttgct
     1801 caatcgagta tggttgacaa tcaataaaac g
//
LOCUS       DAZKNM010000274         1816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000274 DAZKNM010000000
VERSION     DAZKNM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.282"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8M9_002792"
     CDS             <1..828
                     /locus_tag="ACG8M9_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0379485.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="ACG8M9_002793"
     CDS             828..1445
                     /locus_tag="ACG8M9_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379486.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1449..>1816
                     /locus_tag="ACG8M9_002794"
     CDS             1449..>1816
                     /locus_tag="ACG8M9_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379487.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          660 a          259 c          387 g          510 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcgg tgtacaggtt tatccacaaa
     1501 ctcattggaa tgctatagaa ggcaaaccaa caacggtgaa gggagataaa ggcgatcctg
     1561 gccaagcagc aacaattacc ataggaaccg tttctagtgg ttcaaccgct tctgttacga
     1621 atgtaggaac atcatctgca gctagattta attttgtttt accaaaggga gataaaggtg
     1681 atcctggaat aaatgccaca actacagctg tagctacaac tacagcaaat gggttgatgt
     1741 cgtccacaga taaaactaag ttggatggaa ttgcagctgg agcacagaaa aatccaggta
     1801 atgctacaac tacgac
//
LOCUS       DAZKNM010000275         1814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000275 DAZKNM010000000
VERSION     DAZKNM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.283"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1814
                     /locus_tag="ACG8M9_002795"
     CDS             <1..>1814
                     /locus_tag="ACG8M9_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0379488.1"
                     /translation="TETDFSTNGLGILKDCTRCGIYEVANGKYELELDYPLGTRFDEY
                     FENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVVTLVEVDSK
                     TGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLLQYWGGEIK
                     REPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQSEVGTTNRI
                     YGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGCDKPKVSIT
                     VEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSLSEKTLKLE
                     AGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYVVLWPKEKP
                     SNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANFIQTGLIKA
                     DIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTIGTTDSVGN
                     PFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLT
                     VKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          672 a          223 c          365 g          554 t
ORIGIN      
        1 actgaaacag atttctcaac gaatggctta ggaattttaa aggattgtac aagatgtgga
       61 atatatgaag tagctaatgg aaaatatgaa ttagagttgg attatccttt aggaactaga
      121 tttgatgaat attttgaaaa tgactatcaa ataaaagcaa agccaaacga tcaagaagag
      181 tatcatatct tttttattga tgataaagat atagatactt ttttaaatac agtaactatt
      241 tatgctcaga gtcgtacaaa tcgacttgga agacgggtag tcactcttgt ggaagtggac
      301 tctaaaactg gtcaagaagc gatatcaatt attgaaacta aaatggataa aaaatctgac
      361 atacgacttt attctgatat tacggctgtt tctagcacaa tctttgaagc gagaaatgtt
      421 ttaaattgta ttgctggtga acaaggatca ttgcttcaat attggggcgg agaaattaaa
      481 cgtgaaccat ttaagctttc tttgttaaag cgaagaggtc gcaataatgt tggaacgatt
      541 cggtatggaa aagatatgtc tggtttgaag gtcaaattag attggacagg tataaaaaca
      601 agaattattc catatgctga tcctcaaagt gaagtaggta cgactaatcg aatttatggt
      661 tcgccagtag atagtgccta tattaataac tatcctgatg tctatacaga acatgttcag
      721 tttacagaag aacaaggagt aaaagacatt aagagcttaa ataaaatagc taaaaattac
      781 tttaaaacga tcaatcctgg ctgtgataag cctaaagttt ctattacggt tgaatttgat
      841 aagttgactg atagtgaaga agcgaaagaa tttgcgaaga ttagaaatta tggtctgttt
      901 gatacattta aaatatatca taaaaaatat aatatttatc ttgaatcaaa ggttagtgga
      961 ctacaatacg attctttatc tgaaaaaact ttgaaattag aagcaggaga cattcaagtt
     1021 gctttttatc agcaacaagc tgtaactatt caggataaat taaaagatta tgcaacgaat
     1081 aattatatga gtgattttaa tgattatgtt tcttcaatga ttacaggtca aggcagtgca
     1141 ggaggatatg tagttttatg gccaaaagaa aaaccttcca acatctttat tatggataat
     1201 ccagatttag aaaaggcaag agaagtacta agaatgaata aaaatgggat tgctttttct
     1261 aagaaaggat ggcgggggcc ttttaattca gcttggacat tagatagtat atttaatgcc
     1321 aattttatac aaactggatt aatcaaagca gatatctttc aaaattcttt taacaagact
     1381 ggagatgtat tgaaattagt taatggacta ctccaaattt ggaacaacaa aaagaaaata
     1441 atggagttaa ctaaaaaagg aatggaattt tggaattcta agggttcaat tggaacgatt
     1501 ggaacaactg attctgttgg taatcctttt cctggggctt ctactccaac ccctattgaa
     1561 gataattctt tagttattcg tacaaatgga gacggcaaat atattttgat ttctcctaaa
     1621 gtaggaaaag gattagtttt attaggaaat ggtaaagcaa tttattttgg agacttagat
     1681 gtacaaggaa aacttacagt taaaggaaaa gaaataattc ctggacaagg tggtggtcca
     1741 tcgggaggtg gagaatctac tggtgggtat cctagtgaat taaaaacaga tgcagaaaag
     1801 agagcttgga gaat
//
LOCUS       DAZKNM010000276         1795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000276 DAZKNM010000000
VERSION     DAZKNM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.284"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1701
                     /locus_tag="ACG8M9_002796"
                     /pseudo
     CDS             66..1701
                     /locus_tag="ACG8M9_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          582 a          281 c          310 g          622 t
ORIGIN      
        1 cgatttattt aaaatttaag ttatactaga taaagcaatc catgaaagga gtatataatt
       61 aagtaatgca agtaaagaaa aaaataatat tgtccattct cctagcaatt attgctggac
      121 tttattcaat caattatttt agaaatgttc atactatttc tacatcgggg gatattgcct
      181 tccattttgc tagagtcaaa gggttatctt caattttttc tggtccaatc aattttacga
      241 cgtttaatca ttatgggagc ggagtgaatt atttttatcc ctacttgacc ttttttccgg
      301 cagtcatttt ttattggata agtaataatt taatcgttag ttatatctta tatgtttggc
      361 tattgaatgt ttgtactatc atgctgatgt ttcattatgg actaaaattt ttaaaaagaa
      421 tcgatgcggc ttttatattt agctgtttat atacatttta tgggtaccgt acgattgata
      481 tttatcatcg ttctgctatt gctgaagcga ttgcacttac ggttataccg atagttatgt
      541 attatgctta cgcactgatt tatgagaaaa agccatgtgc aattccatta gcagtcagta
      601 tgagcttact catttataca catgttcttt ccacttttat gagtgtaata gccgttggct
      661 tactagtctt aggcggcttt gtagtaagtg aaacaagaaa aaaagaaagc atccggttga
      721 ttgtggagtt gtcaaaggca gtaggaatga cgattctttt aacagcatac ttttggtatc
      781 cgatgttccg acaaatcatg gttcaatcga ttaatgagcc agataaaacg accttacaac
      841 accaagcatt aaatgtctcg gatagtttga ttggtgcgat gaataatgac ctgacaacct
      901 ttacggtagg aatcattggt attatttctt tgatctgtcc actcttcttt tttaaaggat
      961 ttaatcgcaa agaaaaagtg atttacctta gtgcggtagt ctcttggctc ttatccacca
     1021 aattttttcc atggtcttta ttacaaaata cacccattca aatgattcaa tttccttggc
     1081 gaatcttagg ctttcaagta ctgtttggct cgttgatttt atcgttcgtt ttcatgaaga
     1141 gcacatcagc taagcaaaga aaagtagtta atataacggc aattgtttta ttactgttgg
     1201 ctgtatctgc tgcaacaaaa gcaaactatg aacataaagt tgtcactcag agagatcact
     1261 tggtaatgaa tcaagaaaat cttattcgtt acacgacact ccttcccgga ggactttatg
     1321 actatgcacc cacggaagca ttacaataca aagaacattt gcagaatcat gaagtaagaa
     1381 ttggcaagga atggcatact atgccttatc aagtgatgga ttcgaaaata gtctatacag
     1441 tacatgaatc taaaggacaa aaaataacgc ttcctgtatt tgattattgg ggaacagtcg
     1501 caaaagtaaa tggacaaaaa agagagattg ataataggac tagtgtcgat tgaaggaaca
     1561 caaggagaaa caatcattga agtttcttcc atttatcctc caactatgaa gttagctttt
     1621 ttaattagtt tatgtagtta cctctttctt ttaattagtt ttcttatcag gaagttacaa
     1681 catataaaaa taatgatctg attacatttt tttcaatatt agcttactag agaataagaa
     1741 aaagtaagta ataaaccaaa tttaaacttt atattataat aatgaagctt aatct
//
LOCUS       DAZKNM010000277         1766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000277 DAZKNM010000000
VERSION     DAZKNM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.285"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..790
                     /locus_tag="ACG8M9_002797"
     CDS             194..790
                     /locus_tag="ACG8M9_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0379489.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(848..1330)
                     /locus_tag="ACG8M9_002798"
     CDS             complement(848..1330)
                     /locus_tag="ACG8M9_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379490.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1436..1633)
                     /locus_tag="ACG8M9_002799"
     CDS             complement(1436..1633)
                     /locus_tag="ACG8M9_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379491.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          592 a          359 c          269 g          546 t
ORIGIN      
        1 attcctaaaa cacaacaatg aaagcgataa ctaagaaaat ccttacataa cgatttttct
       61 tattaaaatt gttttttgaa cttcgcttat tttttgtgag catatctgtt tacttacttt
      121 ttgtaaggtg ctatacttca tttcgtaatc aattaaaaaa atgcaaaacg caaatcaaag
      181 gagaaatttt attatgacaa cttttactga cactttgaaa aaccgccgtt caatctatca
      241 tctaggacgt aatgtttcac tttctaatga agaattaaca actttgatca aagaagcaat
      301 taaagaaagc ccaactgctt tcaatgctca atcaacacgc gcagttatcc ttttcggaga
      361 tgcacatgaa aaactttggg aaatcactga agaagcactt cgcccattga ctccagcaga
      421 agctttccca aatacacaaa acaaattagc tggtttcaaa aatggttatg gtacagtatt
      481 attcttcaaa gatactgacg tggtaaaagg tctacaagaa caatttgaat tatatgcaga
      541 caacttccca gattggtcag aacaatcaaa cggaatcgca acagctaata cttgggtagc
      601 attagttgat aaaggattgg gcgcaaactt gcaacattac aacccagtta ttgacgaagc
      661 agttgcaaaa gaatggaata tcccttcaaa ctggaaacta cgttcgcaat tagtattcgg
      721 ttcaccagaa acaccggctg aggaaaaaga atacatgaac gatgccgatc gcttccgcgt
      781 atttggataa ttttatttga aataaaaaac agaggttgtg acaaacatct tgtcgcaacc
      841 tctgttttta ttctggatca atcataatat caatctctgc ctcatcgttt aagcgttggg
      901 acaaaaatgg aactaattct tctaatgatg cagaaaattt cagcatcggt cctcgccatc
      961 cgtttcggaa aaaattcatt tttacgattt tagaaccttt tggctgatat aatacaattt
     1021 tattgatctc agaaaaagga acaccttttt tgtcaaagct attcaaaaca agtccttctt
     1081 ctgtgattcc tctactgtcc aacaaaaaac taataaaaat cattgctgac aagatccctt
     1141 ttgccaattc atctgctgta ggatgactga tgagtgtata ccaaatgact agtgacacta
     1201 agaagggtat caccaagtag acccatcgat ttcgcacagt ccgataaata atttcttttc
     1261 gaaccagcca aagaaataaa aacatcacaa caacgaataa aaacaataga accgtaaaga
     1321 atgttaccat gacaactcgc ctcgcttttt cttctccaga aaataggaaa agaatactga
     1381 tttaccttct caaagaaatt ggacttgcag cacgccgcac gtccaattgt tttctttatt
     1441 caaatgtatc atattttgca acaaccatgc ttgctttttc atctaataat tcttggtcct
     1501 ctgcagcaca ttgggcaact ttgaccacgg cgcagtttgt catttttgat acaacttcac
     1561 caatgaccgt ttttgtaaaa ccaattggtt tgcatgcata ttgttctcct aaattaatca
     1621 tttcttccgt catcaaaatc ctcctaaact gctggaactt ttctccacat taccactgct
     1681 atttctttgg agatttaact cattgatttt tatttacagt taaaaatcaa ccttacaggc
     1741 attatagcgt actgttttcg ttttca
//
LOCUS       DAZKNM010000278         1754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000278 DAZKNM010000000
VERSION     DAZKNM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.286"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..1147)
                     /locus_tag="ACG8M9_002800"
     CDS             complement(128..1147)
                     /locus_tag="ACG8M9_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0379492.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1144..1368)
                     /locus_tag="ACG8M9_002801"
     CDS             complement(1144..1368)
                     /locus_tag="ACG8M9_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0379493.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1365..1658)
                     /locus_tag="ACG8M9_002802"
     CDS             complement(1365..1658)
                     /locus_tag="ACG8M9_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379494.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          491 a          358 c          298 g          607 t
ORIGIN      
        1 tatcgcatag cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac
       61 tattttatcg aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga
      121 gctattttta tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac
      181 cgcctttagt gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat
      241 tagggaagtt acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag
      301 cttgatttac atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag
      361 tattgtaatc cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta
      421 catgcaaatt tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat
      481 ctgtcagtgt aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag
      541 aagttgttgg attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac
      601 ttacgcccat ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg
      661 ttacccataa atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa
      721 taccatattt cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa
      781 acgttgcttt atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat
      841 acgaaccagc accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt
      901 ctcccacaat ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat
      961 tgcgtcctgt tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta
     1021 cttgtgagct accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc
     1081 cgttaattgg caataaaaaa agagccgtta acaggctcat cgcagtaata gtaattttct
     1141 ttttcatttg tttcctcctt cttcgctttc agccgagaac attttgtagg ttcgatttga
     1201 tacacccaac acactcccta aaaacgcgcc aaaaccagta atgatgacaa cacagatatc
     1261 tgtgtactgc caattgagcg ctttaccaac taaccccacg aaagtagcta gtgcgggaat
     1321 aattaccagt gcgaaccatt ttagtacttc gaacgtttta ttattcattt tcttctctcc
     1381 ctaaacaaag ttttgatttg ttgcgtgtgt tccaccaatt tttctgtatg tgtatctaat
     1441 ctttcatcgt gtttctttag ttcttcatga atcatcaatc gatctgattt gctcgattct
     1501 aaatctttag tcagcaaatc taaattgtgg cttacttttg aaagagtctc agtaattttc
     1561 gagaaagatg cagcaatcgg tctaattact aataaaatca aagaaacgat agcggttatt
     1621 gatcctgcta tcattcccca ttcccctaaa ttaatcatgt gacaactcct ttaccttaaa
     1681 taaaaacgca tcaattaaga tgcgctctta tctttattaa tgattttatc tgcttcttcg
     1741 tctgtaatgc ataa
//
LOCUS       DAZKNM010000279         1740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000279 DAZKNM010000000
VERSION     DAZKNM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.287"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8M9_002803"
     CDS             <1..189
                     /locus_tag="ACG8M9_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0379495.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="ACG8M9_002804"
     CDS             complement(1055..1585)
                     /locus_tag="ACG8M9_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0379496.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..1740)
                     /locus_tag="ACG8M9_002805"
     CDS             complement(1582..1740)
                     /locus_tag="ACG8M9_002805"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0379497.1"
                     /translation="MAFYFSAIIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLIL
                     ITKRDGER"
BASE COUNT          556 a          342 c          206 g          636 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
//
LOCUS       DAZKNM010000280         1684 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000280 DAZKNM010000000
VERSION     DAZKNM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.288"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            399..1274
                     /locus_tag="ACG8M9_002806"
     CDS             399..1274
                     /locus_tag="ACG8M9_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0379498.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1419..1571)
                     /locus_tag="ACG8M9_002807"
                     /pseudo
     CDS             complement(1419..1571)
                     /locus_tag="ACG8M9_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT          602 a          312 c          260 g          510 t
ORIGIN      
        1 cacattctaa gtaaacaaag cggtaaaaat atcttttatt cccttagtta atccgcttac
       61 tacctactgt acaaagcaaa ttgttgttca aactgctgat cagaacgctg cttggatttt
      121 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
      181 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      241 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      301 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      361 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      421 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      481 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      541 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      601 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      661 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      721 gggagctttt aacagagatt cttatagaac atccgcaatt tatcgaacgc ttcaaagtta
      781 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      841 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      901 agttcatcca agaagcagtt gaatcgctca ttcagcaaaa tgcctatctt cccgcattcg
      961 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
     1021 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
     1081 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1141 cctcttattc aaaacgctac tccataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1201 tttctaacca aaagaaaaaa atagataaaa aaactaaaga aacggtaaag aaagacgatt
     1261 tcaatgaaaa atagcaagta aacgataaca accatcctaa atccacaaga aatacagcag
     1321 accttgcttt aaaaacacga atcatgcaaa ggatgaagta aagaagactt aacgcgtacc
     1381 caatattagt catttgacta ttaggtacgc gttttgtttt ataagtcatg acaaatttcc
     1441 ttcgttgctg ggtagatttt ccggcattgt tgatagattt tatgatactt ttctacattt
     1501 tgtaagagag gttccactgc atctgtatat tggataaaat tctgacaaca actttcaggt
     1561 gtgtcgaacc actctaatct ttaaagataa atattaaaat aacgattaat aatattaaca
     1621 gtctttattt ttcaccacca aaaacctatc tttgcatata ctaaatagac gtatatacac
     1681 agat
//
LOCUS       DAZKNM010000281         1649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000281 DAZKNM010000000
VERSION     DAZKNM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.289"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1600
                     /locus_tag="ACG8M9_002808"
     CDS             134..1600
                     /locus_tag="ACG8M9_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0379499.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          553 t
ORIGIN      
        1 tatgatattg ccattaaaaa ttacttgctt ttatagaaaa ttagtttata tttcttaaat
       61 aagtttttta gttaaatttt tcggtaaaga tgtataatat tttctgaaaa tttaaagaat
      121 tggggtatag attatggaaa aacagcaaga caagggcttc ttcgggcagc cgaagggatt
      181 gtcaactttg ttcttcactg agatgtggga acgatttagt tattacggta tgcgtgcatt
      241 acttatctat tatatgtacg atacagttgc caacggggga cttggattac cacgagcaac
      301 agcattagct atcatgtcga tctacggatc tttgatctac atgtctagta tcgtcggcgg
      361 atgggtatct gaccgagtgt taggagcaaa acgcactgta ttcttcggtg gagttttcat
      421 tatgcttggc cacatcgtct tggctttacc atttggtgta agtacattgt ttgtttcgat
      481 ggcattgatt attatcggaa ccgccttttt gaaaccaaat gtttctggca tggtcggaca
      541 tctttattca aagactgact tgcgaagaga tgcaggattt tcgatttttt acatgggtgt
      601 caacttaggg gcattattag caccactaat cgttggtaca atcggtcaag aatataacta
      661 tcatttaggg ttctctttag ctgctattgg gatgttcttt ggattattac aatacatcat
      721 ccaaggacgc aagacattag acggagtggg tacaacaccg ccgaatcctt tgacaactga
      781 agaacgtaaa ggattcatcc gtaacttgat catcgcactt gttgttatcg ctgcagtatt
      841 cggcggggca caactaactg gtcatctgac aattgacttt tttgtcaata caatcagtgt
      901 attggggatt atcttaccgt tgtattactt tatcaaaatg ctgacttcaa aagatgtaac
      961 tgctgaagaa aaaccaagag tatgggctta tatcccatta ttccttgcag caatcatttt
     1021 ctggtcatta caagaacaag gttcatctat cttagcttta tttgctagtg aacgtacaca
     1081 atctacgcta tttggtgtaa cgatccaagc aagctggtat caatcattga atccaatttt
     1141 catctttatt ctgacaccag tattcgtaac gatgtggacg aaacttggtg aacgtcaacc
     1201 atctacagta gtgaaattct ctcttggact tttatttgca ggattatcgt acttgctatt
     1261 gatgttgccg ggtatgcttt atggtacaag tggaaaagta agtccattgt ggttgttggg
     1321 aagtttcttc atcgtggtta ttgctgaatt gtgtctttca ccagttggtt tgtctgtgac
     1381 aacaaaatta gcaccacgag catttgaatc acaaacaatc gctatttggt tcttagctga
     1441 tgcttcttca caagcaatca atgcacaaat tgcacgcttc tacacaccgg gaacagaatc
     1501 tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc tatttactgt
     1561 gaaagaacca atcaaaaaac taatgggctc tattcattaa tcaatgaaca taaaaacagg
     1621 aattgcttat ttgcagcagt tcctgtttt
//
LOCUS       DAZKNM010000282         1619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000282 DAZKNM010000000
VERSION     DAZKNM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.290"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1384
                     /locus_tag="ACG8M9_002809"
     CDS             56..1384
                     /locus_tag="ACG8M9_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379500.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1395..1604
                     /locus_tag="ACG8M9_002810"
     CDS             1395..1604
                     /locus_tag="ACG8M9_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379501.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT          560 a          265 c          306 g          488 t
ORIGIN      
        1 tttctttatc attggttata attaaattat aaaaagattt gaaagaggtg agaaaatgat
       61 tggcgaagat aaattattga tgactatcca gctcacagac ttaaatgaag aagaaaaaaa
      121 acaggaaata agcgattggg ctattttgac acgtctgttg atccagccaa catttatccg
      181 ctctttcaca caacaagcgg atccgtatga tcttcgaaag cttcaagaaa aaataatgct
      241 agcatcggta agagacgaaa gttggttagt tgtaggaaat gacgagaacg aatgtagttt
      301 tcgcttggaa gataatcaat tattgataaa aaatgtattg tctatctctg ttttcaaaga
      361 aaagcaattt ttgattcgag acttcattca gaaaaaaatg attgctcatg gggtgttcgc
      421 ctatatgcga gcttatagcg agtttatcta tcacaataca aaacagatct ctcaacggct
      481 attgtttgag aaaaaggatg aaatcgaaca tttacctaaa atgaaacaac aaaacgggga
      541 agtagtagtc gattgcaacc aatttccagg atacgatctt ttttaccaag gtttatgttt
      601 cacttcgtgt tgggaaatgt attatagtcg ctattatcat caaatcatcc ctaaaccgat
      661 ttttctcgac atccaacaag tagaaaaagt gaaggaatta gaaaacgaag tcatatgtat
      721 ccagctttat cgagacccct ttaactggca aaagccaaac aatcaaatgt ttcaatccta
      781 ttttagggac cagcttgggt ttgatcatct tgcttgggac aatggtgtcg gtttattgaa
      841 accgccattc gttgaataca tttacactga ccatacgatc caatctgtcc agtatcagaa
      901 tgcacaaatg caacctgttc ctaaaaagaa tgcttcattt tttgtaacaa aaagttatga
      961 tatccaacaa ggagattata aagaaaggcg tgttcgcgga acactaaatg ctcaggcgta
     1021 ttttccttgg gttgacgaga atcgatcacg catgatgtgc tataaagtta tcgatccgac
     1081 tgttgctttg gataatggaa tcgaagctta ttgttattat attagggaat atctagaagt
     1141 agaggtaaca gatgaaaaat atcaagagta tcttttatcc ttaagaatct atgttccttc
     1201 agaatccctg tcagaattac cgttaaagga aatcaaacat agactctctg atgttacctt
     1261 caaacgaata aagaaaaagc ggcgaagtat ccaattcgat gtcaagaaag gtgagcagca
     1321 tctgcgtgtg caatttttag attaccgaga attagaacaa ttgattactt tacaaaaaat
     1381 atgaggtgaa gaaaatggat aaacaactct attatcgaat aatggttccg gcatcattat
     1441 tatccgtctg tctctttttt gttcctaatt ttcctattcg atcagtcatc ggttttttgg
     1501 tcatgtttgg cgggatggga ctttattatt atcttcttca taaggaaaga aaacctgctt
     1561 ctgtttatat gaaaaaaaca agaagcgaaa ataaaaaacg ataaaacggc ttgatgata
//
LOCUS       DAZKNM010000283         1602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000283 DAZKNM010000000
VERSION     DAZKNM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.291"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..461
                     /locus_tag="ACG8M9_002811"
     CDS             39..461
                     /locus_tag="ACG8M9_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379502.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            471..674
                     /locus_tag="ACG8M9_002812"
     CDS             471..674
                     /locus_tag="ACG8M9_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379503.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            885..1469
                     /locus_tag="ACG8M9_002813"
     CDS             885..1469
                     /locus_tag="ACG8M9_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0379504.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          565 a          227 c          295 g          515 t
ORIGIN      
        1 aaaacgtttt cttatttttg atttcgaggt gatagacaat gaaaaataag tttattttat
       61 ctttaggtat tttaactatt ggaattagtg taactggaac tacaattcct acatttgctg
      121 atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact cctttagata
      181 ggaacggaat agccattggt aacccaacta acgacttaac tgttactgaa aattatttaa
      241 tagtttctcc attacttgct cgagcagctt ctaaaacgat acgagtgaca ggaacgatga
      301 tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg aaagaaaaaa
      361 gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca gctggaaaca
      421 attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt atgccagaat
      481 ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt aatttaccga
      541 aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa attcaaaaat
      601 caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc
      661 aaaaagaaaa ataatgattt taaaaaaaga gtgtctagtc gacattcttt ttttctaatt
      721 gaaagtgttc ataaaatcac ccatttctgg acactttctt tttttgcttt atttttcaaa
      781 aaaacaattc atttcatgtt cataaaccca taacaatatc tatgttcagt taaactatat
      841 ggataaagtt atgttacaat aaaattaatg aaggagtgat ttttttgaaa ataggatacg
      901 cccgggtatc tactggttta caaaatttgg atttacaaga agacagccta aataaatttg
      961 gttgtgaaaa aatttttact gatcatatga gtggggcaaa aagtaatcga ccaggattag
     1021 aaatggccat tgattttgtt cgctctggcg atacacttgt cgtttggcgc ttagatcgtt
     1081 taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg actgaatgag cgtggtgtga
     1141 gttttcatag tattcaagaa aatattacga tggacaaatc aagttctaca ggacagctca
     1201 tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa tttgatcctg gaacgatccg
     1261 cagcaggacg agcagctgct cgtgcaagag gacggtttgg tggtcgtcct gaaaaattgt
     1321 ctgaacaaga tttggaatta ctgaagactt tagtggatag cggtacacct attaagacta
     1381 ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg ttatttggca aaaatggagg
     1441 agaaaaatgg aaagacaaat tctcattagt ttttgtcatt taatgctgaa aatttttgcc
     1501 atttatctag aaaagtgaca atagtatgag aattttttgt aagtgtctac tgataactta
     1561 ttctcaaatg ttgtaaggat tttattcatt tgttaatatt aa
//
LOCUS       DAZKNM010000284         1597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000284 DAZKNM010000000
VERSION     DAZKNM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.292"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACG8M9_002814"
     CDS             complement(1016..1357)
                     /locus_tag="ACG8M9_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0379505.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACG8M9_002815"
     CDS             complement(1357..1587)
                     /locus_tag="ACG8M9_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0379506.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          525 a          263 c          226 g          583 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcact
//
LOCUS       DAZKNM010000285         1586 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000285 DAZKNM010000000
VERSION     DAZKNM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.293"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1509)
                     /locus_tag="ACG8M9_002816"
     CDS             complement(28..1509)
                     /locus_tag="ACG8M9_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379507.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          502 a          216 c          280 g          588 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gtgtta
//
LOCUS       DAZKNM010000286         1583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000286 DAZKNM010000000
VERSION     DAZKNM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.294"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..266
                     /locus_tag="ACG8M9_002817"
     CDS             3..266
                     /locus_tag="ACG8M9_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0379508.1"
                     /translation="MWIFDRSRNASIGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLS
                     STLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(470..835)
                     /locus_tag="ACG8M9_002818"
     CDS             complement(470..835)
                     /locus_tag="ACG8M9_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379509.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1043..1525
                     /locus_tag="ACG8M9_002819"
     CDS             1043..1525
                     /locus_tag="ACG8M9_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379510.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          549 a          214 c          285 g          535 t
ORIGIN      
        1 atatatggat ttttgatagg agtagaaatg caagtatagg ttttagaact aaacgaagtc
       61 tgtcttctaa aaaaaattgg gtatactcac aaactatttt ttatggagga attgttttaa
      121 ttagtttact ttcttcaaca ctttattctt tgaatattat agatgtttcc acctcaaatt
      181 caatatctat tattggaatc attattgctg caattattac acaactgttt ttagtgtttg
      241 gagagaagaa aagaagtaaa aaatagaaaa actagtgata gttttggtga tagtgatatt
      301 aaaaatcaga aaagatagaa aaagcaggag atagataaaa tgctgatatt tcagcatttt
      361 ttaagagtat aaaagagaaa aaaagtaata aatgtataca tgatataaga aaaacatata
      421 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      481 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      541 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      601 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      661 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      721 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      781 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
      841 atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata
      901 atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc attatctcta
      961 aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat gatgagagaa
     1021 aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt
     1081 tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg
     1141 tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga attttccagc
     1201 tttgactgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa
     1261 tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat ctatgttttc
     1321 tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattc
     1381 agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc
     1441 atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc
     1501 tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaagcat atgagctgcg
     1561 taaaatgcat gtttctcata tgc
//
LOCUS       DAZKNM010000287         1564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000287 DAZKNM010000000
VERSION     DAZKNM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.295"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..471
                     /locus_tag="ACG8M9_002820"
     CDS             115..471
                     /locus_tag="ACG8M9_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0379511.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            481..1380
                     /locus_tag="ACG8M9_002821"
     CDS             481..1380
                     /locus_tag="ACG8M9_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0379512.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          520 a          279 c          324 g          441 t
ORIGIN      
        1 gaaatcataa taaacgaatg acttactttc caataaattg agaacagtca gaaccagaca
       61 aattagttgc atctgatgaa taaagtgatt attctatgac atgaggtgat acagatgaaa
      121 ttagaagtga caaaagaagc acaagaagta ttgaagaata aattagaagc agacgatcaa
      181 cttttattag atatcgaaaa cggtgacggt ccatttgcag aaagccaagt cagttgccaa
      241 ttggatacgg catttcgatt gatcattgtc aaaaaagata cacaaacaga tctaagtctt
      301 tattctgtag tggctgatac accagttgga ccaatcaaaa tcaaagacag tgcaatactt
      361 tacatggatg atccatccac acttgcttta gaaccaactt ataatagttt acaattaaaa
      421 ggaccaagcg gcttgagaaa aggcaacttg caagttgttc gaaaagacta gagggataaa
      481 atgggaaaat tggcgatcat cagcgaccta catgtggaca tcaataaatt cggcgaatca
      541 gagctgatgc agctttggca agttttgcaa aagcacaaga tcactcggct tcatctagcc
      601 ggagatacgg ctaataaagt agaccgttgc ctagctgttg tcgaattttt cgtcaataaa
      661 ggattatcta cgacatttca tttcggcaat cacgagttgg cagatgtgcc caatgaacag
      721 atgatggacc attttccaga cccacatttt ttaaatgaac ggtatattgc tttaaatgag
      781 cagaccgttc ttttagggat gaatggctgg tacgactatc agttttccga attgaaagat
      841 tacgaggcaa tcgtacgaat gaagcgtttg tattggtatg atcggttcat ccagcgtggg
      901 aaaacagatc tcgaggtcaa tgcagaaatg cttgcagctg caaaaaattt gctagaccag
      961 ctggaagcaa aaaaacttga ggtgatttta tccactcatt tcgttcctaa acgggcattt
     1021 atcatgtatc aatctgctcc ttatgagcgt tggaacaaat taaatgcatt tttaggatca
     1081 gaatcattcg gagaattact tgaccgttat tcaaatatca aacaagtagt ttttggccat
     1141 acgcatcgtc gctttgaaga taaatccatc ggtcacacga tttacagctg cagacctttt
     1201 ggttattatt atgaatggca gctgacccga gattttgtct tgaaagaaca gttgctagac
     1261 agctatgatc ctatgaaatt acgtgtcctc ttgcgtgccc atcacccagc ttttcaggcc
     1321 tatcaaacac aacatttgca agaggaattt gaacaagcga tgacaatcgt tccgtattga
     1381 gacagcatta gtaatgtgta aaagaaaata ccttgtaaaa cataaggata agtaagagca
     1441 gtagatccat ttatcgtgga tatgctgctt tttttgcgga aaataatcag cgaatgttta
     1501 tttgatcgga tgatttgtaa ctcgcagagt actagatgca agcgtgtatt ctttttttga
     1561 acga
//
LOCUS       DAZKNM010000288         1540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000288 DAZKNM010000000
VERSION     DAZKNM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.296"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1490
                     /locus_tag="ACG8M9_002822"
     CDS             <1..1490
                     /locus_tag="ACG8M9_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0379513.1"
                     /translation="QQQTIQNLTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNL
                     FSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGS
                     QMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSL
                     GSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGER
                     LRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPK
                     TFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQ
                     LDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACK
                     LRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAE
                     LNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          520 a          280 c          318 g          422 t
ORIGIN      
        1 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
       61 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      121 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      181 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac
      241 acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt
      301 gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc
      361 aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa
      421 aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct
      481 taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg
      541 cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata
      601 tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa
      661 gagtctatct taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg
      721 aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc
      781 ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca
      841 aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg
      901 tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag
      961 gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg
     1021 tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag
     1081 aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa
     1141 aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta
     1201 tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc
     1261 tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag
     1321 gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc
     1381 tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc
     1441 aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa aaacgccaaa
     1501 cattcccgat tctattctat aggtcgggtt atttgacgta
//
LOCUS       DAZKNM010000289         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000289 DAZKNM010000000
VERSION     DAZKNM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.297"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8M9_002823"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8M9_002823"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKNM010000290         1520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000290 DAZKNM010000000
VERSION     DAZKNM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.298"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1198
                     /locus_tag="ACG8M9_002824"
     CDS             <1..1198
                     /locus_tag="ACG8M9_002824"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0379514.1"
                     /translation="KGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCV
                     KKDGTRFSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKK
                     VGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLC
                     LSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTN
                     EYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQ
                     FTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKN
                     TKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVT
                     GLNIET"
     gene            1310..>1520
                     /locus_tag="ACG8M9_002825"
     CDS             1310..>1520
                     /locus_tag="ACG8M9_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0379515.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSN"
BASE COUNT          607 a          191 c          250 g          472 t
ORIGIN      
        1 aaaaggagaa gtaagtaatt tagaagatct tttttatcaa aatagaatga cactaaattc
       61 tgagataaaa aagttatttc tagagacaga agttgacaaa attgatattc aaaataaaaa
      121 gattagttgt gtaaaaaaag atggtacaag attctctcaa gaatatgaca aagttatatt
      181 agctacaggt tcaaagcaaa ttatgctaga tataccgggg aataatttag ctaatatttt
      241 ttcgataaag aattttccaa atgcaaaaga gatttttaaa gctttggatg atagtactat
      301 taaaaaagta ggaattattg gagcaggata tattggagtt gaaattgctg aagccataag
      361 aaaaagaggg aaagaagtat atcttttcga tgttgcagat agagtgttgt cgacttatta
      421 cgaccgtagt ttttcagata aagttgaaga aatactatca aaaaatggaa tacatctttg
      481 tctatcagaa agtcctaaaa aatatttagg aagaaaaaag attgaaaaaa tagtcactac
      541 aagtggtacc caatactcta tggatatggt cgtacaagct ataggatttc tatctaatag
      601 tccaattggg gaaaaggaac tcttgagaga tgttagtgga gcatatctaa ctaatgaata
      661 ccagcaaaca aatataaaag atatatatgc tattggggat tgtgctacaa ctttttttac
      721 gtccattaat agaagtagtg ttgatttttc tatttctaat gctctaagaa cagcttatat
      781 tgcaactcaa catattaaca atcaagattt tacaccttct ggttcccaat ttacaaatgg
      841 cttttctata tttaattaca atttttacgc agcaggtttg aatttaaaaa cagcacgatt
      901 attaagtgag tctatagatt atatagaaat agaagatttc attagacctg cttttttgag
      961 aaaaaatagt cgtgtaaaat taagaattag ctttgaaaaa aatacaaaga gaatagtagg
     1021 tgtacaactt tgtagtcaag aagatttatc tggactatta tcaatgtttt ctcttgctat
     1081 agaagaacaa attacattag acaaattgaa aatgttagat tatttatttc atcctagttt
     1141 tagccaaccc tataatttta ttttaaaggc agtaacagga ttaaacattg aaacataatc
     1201 gttactaaat gaaatcaaga aatatactgt attactcaat tcattgaaaa tatttatcat
     1261 aatttttaat agaaaacact attaaagaga aaaaagagag gtaaatcaaa tgaaaaaatc
     1321 tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa cacctttcca
     1381 tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg agacgactga
     1441 aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt catcaagtga
     1501 aagtagtacg acttcaaatg
//
LOCUS       DAZKNM010000291         1489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000291 DAZKNM010000000
VERSION     DAZKNM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.299"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..>1489
                     /locus_tag="ACG8M9_002826"
     CDS             177..>1489
                     /locus_tag="ACG8M9_002826"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0379516.1"
                     /translation="MIVSYIIYVWLLNLCTTLVAYHYGERFLKQKKAAFLFSCLYIFS
                     AYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSVKLALSMSLLVYSHVL
                     STLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAFWYPMFEQMLY
                     QKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSLCIPLILFKKLTNIEK
                     KIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFKNRRY
                     NKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLY
                     DYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLG
                     TNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYL"
BASE COUNT          488 a          231 c          249 g          521 t
ORIGIN      
        1 gtatttatac cactggagat ctttcttttc acttatctcg aatcaagggg ttatcgacta
       61 tttttaccag tccgattaat tatgagacat tcaattatac tggatatggt gtaaattatt
      121 tttatccctt tctgactttt tttccagctg taatgttata ttggatgaca aaaaatttga
      181 ttgtaagcta tattatttac gtttggttat tgaacttgtg tacaacactc gtagcttatc
      241 attatggcga gcggttttta aaacaaaaaa aagcggcatt tttatttagt tgtctctata
      301 ttttctctgc ttatcggaca gtagacatct actatcgttc cgcaattgcc gaagctatcg
      361 caataacact tgttatccct gtattatttt atgcttatca aatcatctct ggaaaagaag
      421 aaaagtatcc gtctgtaaag ctagcattga gtatgagttt gctagtgtac agccatgtac
      481 tatcaacatt aatgagtaca gcattaatta tcatttttat ttttattcgt ttggtttcta
      541 aaggtttcaa gaatgcagat tttattgcta tatttaaaaa attatttagt gcggcaggaa
      601 tgacattggt tctgacgagt gctttttggt atccgatgtt tgaacaaatg ctctaccaaa
      661 aaatcaacaa acctagcgtg acaaatttat atgctcatgc atctaatgtg ttcgatagtc
      721 tgactgaagc tatgaacaac gacttaacta cttactcaat gggactagtt ggtctgttat
      781 ctttatgtat tcccctgatt ttatttaaaa aattaacaaa catagaaaaa aagatttatt
      841 atggtacgtg tttgacttgg ctagctacaa ctagtttggt gccatggtat cttctgcaaa
      901 atacgccagc caaattgtta caatttcctt ggcggatctt aagcttgcaa ataatattct
      961 cttctctgat attaacgatg atatttttca aaaatagaag atacaataag acaagagaac
     1021 tattttattt atttatgact gttttgttat taatcacttt aactgtttct agtaaaaaaa
     1081 atttcaatca aaaaataatt tctcagccgg gacatattgt tgtaaacaaa gaaacagtag
     1141 aggcgtatac cactaaagga atggattatt ttttatatga ttattctcct atcgcttcat
     1201 tggagaataa ggagcatata ataaaacacg aagcaaagat tggcagtcaa tgggtaaacc
     1261 ttccttataa aacaactggt agttctatca tatatagagt aactaatgag aagagccaag
     1321 taatcacact tcctgtatat gcttatttag gaacgaatgt agaaataaat catcaacctg
     1381 taaaggagca ggtgaatgct aatggattgg tacaaataag cggagtcaaa ggagttgctg
     1441 atattaaagt aagcacaagt tatacagtaa cgactattat ttcttatct
//
LOCUS       DAZKNM010000292         1484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000292 DAZKNM010000000
VERSION     DAZKNM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.300"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            224..814
                     /locus_tag="ACG8M9_002827"
     CDS             224..814
                     /locus_tag="ACG8M9_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0379517.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            841..>1484
                     /locus_tag="ACG8M9_002828"
     CDS             841..>1484
                     /locus_tag="ACG8M9_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0379518.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIP"
BASE COUNT          517 a          168 c          294 g          505 t
ORIGIN      
        1 ggtttaaata actggaaata tcggaaaatg ggcattatac atcacggaca tcccttcttt
       61 tggaaaaaat gttacgattt ttataagtaa tattgtatcc ggtggaatca acgatgaaga
      121 taacatatcc atcgttgtag attcagtagc attgaatgat gctcttgttg caagaaatcc
      181 gaataaaatt gttaagtata aattgaatta ataggagata tgaatggaaa ttagcagtaa
      241 agaatattta aaagcggttc ttcgtatgga aaagaatcta tatatagaat ctaaaggaca
      301 acacacaaaa ttactaaaaa taatgaaatc acatccggac tacaacgctt ggcggtatac
      361 tcgtcgaatg agaattacat cttattatta ttcaaagaga aagaaaaata tcatttatgc
      421 gattatgtat attgtgtctt gcagaagaat gaatagtttg ggaagaaaat gtggtataga
      481 gtgtggtgaa aatgtatttg atgaaggtgt aagattttac catacacaag gaattgttat
      541 taatggtaac gccataatag gaaaaaattg ttacttatat gggaataact gtattggaaa
      601 tgatggaatc gatcctaagt gtcctgtaat tggaaataat gtacgtgtat gcgtgggtgc
      661 aaagattatt ggtaatgtaa aaatagctaa caatgtagtt gttgcggcag gagctattgt
      721 tatcaaagat tgtctagaga atggtgcaat tcttgcaggt gtaccggcaa aaataatagg
      781 gtatgttaat ttaacaaaag tatttttaga ataattttta gcgaaagaag gtgaataacg
      841 atgcgtattt caataaaaag atggtcagga gcagattgga cattgttcac gttggcattt
      901 tttgcaatct ccatagttct tgtatatact tttaacgccc caagagcaat tttatatttg
      961 ggagatataa ttaatatctt tgtgttatta aatgcactta gctttagacg cagaaaagga
     1021 atatatgcta gcaaaaatga gatgctgata gttttgtttt tactagtttc tatttgtagc
     1081 tcagttatta atttagaaaa tataggattg attatctggg ggatacgaaa taacactcgt
     1141 atgttcatag tatatataaa ttgtgcaaca tatttaacaa ttaaagatta ctataaagta
     1201 ttcgctataa ttgaaaaatt attctggatt tcactgccac tttgtttaat tgaaagattc
     1261 atggtcacct atccaactgg aacaattgta ggagatatga taggtggagt tttttggaat
     1321 tattcaggtt gtaatatgcc attgaattta attttgtgtg tgacagttac tgttgttaca
     1381 atcggatatt tcaagggaga aaaaaatttc atccatttta ttttatgtgt ttgtgcagca
     1441 ctttttatgt ctgcattagc tgaattgaaa gtttttattc ctga
//
LOCUS       DAZKNM010000293         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000293 DAZKNM010000000
VERSION     DAZKNM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.301"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8M9_002829"
     CDS             <1..828
                     /locus_tag="ACG8M9_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0379519.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="ACG8M9_002830"
     CDS             828..1448
                     /locus_tag="ACG8M9_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379520.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DAZKNM010000294         1447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000294 DAZKNM010000000
VERSION     DAZKNM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.302"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8M9_002831"
     CDS             131..505
                     /locus_tag="ACG8M9_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0379521.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8M9_002832"
     CDS             complement(619..1248)
                     /locus_tag="ACG8M9_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0379522.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1385..1446)
                     /locus_tag="ACG8M9_002833"
     tRNA            complement(1385..1446)
                     /locus_tag="ACG8M9_002833"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          485 a          221 c          255 g          486 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagcta
//
LOCUS       DAZKNM010000295         1438 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000295 DAZKNM010000000
VERSION     DAZKNM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.303"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..347)
                     /locus_tag="ACG8M9_002834"
     CDS             complement(<1..347)
                     /locus_tag="ACG8M9_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379523.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTK"
     gene            complement(358..1026)
                     /locus_tag="ACG8M9_002835"
     CDS             complement(358..1026)
                     /locus_tag="ACG8M9_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0379524.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1156..>1438)
                     /locus_tag="ACG8M9_002836"
     CDS             complement(1156..>1438)
                     /locus_tag="ACG8M9_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0379525.1"
                     /translation="ILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVF
                     NEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          524 a          241 c          195 g          478 t
ORIGIN      
        1 tcttttgtaa atccaaagtt ttccatcgaa aatactaact cactcgttgt atttgttgta
       61 aagaattgtc ctctaggatc ttgacttacg tagccaatat tttttgatat actttcttgt
      121 gttaaggatt gtagcgagtt attttcaaat aaaatttccc caaccaattt tccgtcataa
      181 agtcttggaa taagtccatt cattaacttt aatagtgtgg atttcccact cccagaagaa
      241 ccagtaatca atgtaagact gccttttttt attgaaaaat taaggtcaac aagcgcttgt
      301 tccttaacat ccttatatga aaaagaaaca ttgttaaatt caaacaattc tttcacctca
      361 aatcaaaata cttaagaaaa aaatagtaac ggaaaacagg atgatatagt ctaataagct
      421 catgtttgtt ttatagtaag aacttctttt cacaggagat tctatcccct ttgttattgc
      481 attagcggtc atttcttcag caatcatagt tgcacgtata agaacgggaa ctaacagaaa
      541 ttccaaatat tgcagaggct gaaaaaaaga caagcgaatg tttctcattt ttaaagattc
      601 tttaatcatt cttatttcag aacgataagt cggaaagaat ctgatcacta ctgtaatact
      661 taacattatt tttttcggta aatgtatttt ttctaaagag gataataatt cattgctagt
      721 atagacacta accaacaacc atgagatcat tacgaccggc aacattcgaa atacaatata
      781 taaggatgtc gcaaaaaata tagtaaaaaa ctctatcttg atagcatcaa taattttcat
      841 tactaaaaac agaactgaat aaatcaagat gaaataaata catgtcttta ttttacgaaa
      901 catcaatact aaaactgtag caataatgaa acaccccact tgaaatatat ttccctttcc
      961 taaaaaaata atgaccggaa aaaaaatatt taaagtaatc aaggttaacg gattgatttt
     1021 tgacactctt tcacctccaa aaaataagat cgcactgtta tcttaagctg ataaaaatgc
     1081 accgttattc agataacagt gcgatcttat caaaaagcat aactgagaac aattctcaat
     1141 gcaagctttt tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa
     1201 tcttctggtt aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa
     1261 tgtagcgtag aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg
     1321 attgatcatt ccgatagtct ttaacttatc tagacaacag actgtctttt tcgtcacaaa
     1381 tgatgtaatt ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaatc
//
LOCUS       DAZKNM010000296         1429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000296 DAZKNM010000000
VERSION     DAZKNM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.304"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(401..1015)
                     /locus_tag="ACG8M9_002837"
     CDS             complement(401..1015)
                     /locus_tag="ACG8M9_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0379526.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          465 a          275 c          238 g          451 t
ORIGIN      
        1 tggacaattt tgttatatca ggtaaacttc attgcaataa tgataacaaa tgtactataa
       61 ttactttgta aagagttttg gggaagactc tttcatcaac gtgtattaag agacaagaaa
      121 gaatccatat ggattctttc ttgtctcttt ttccactact ttttattttt ctgacactac
      181 atattcttat aactcatttc tcataactgt gaaacaactt ataaaaataa caaactttaa
      241 acaaaacaag cttaaaatca aagatgatcc atcttaaaat aactaaaaca aaaaaagtag
      301 tcgttgttaa tcctcattaa taatcgtttt ctttttttca aacgctccat ttttatgtgg
      361 aacattattt ttttaaggaa ctttgtcaac atgtggataa ctattttcca cttgtgtcct
      421 ggactagctg ttttgctgta aaatacaaaa cttgctgcag caaaaagatc gttgcaagag
      481 aggctgttcc gcagatcgct ggatctagat ttggcaaagt caagactgca tcatgtaaat
      541 tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa tcgcaaacca ctgctatgtt
      601 gcgatttgat acttcgtgac aaatcaaatc cagtaacaat gattaatgga tgaccatttt
      661 ctttgatcca ctgagctagt tcgatgattg ccgggtttct tccttcattc gaaagaagaa
      721 gaaaaatgtc ttctgatttg acagaaagct gatcaataat tgcctgtcct gcaccttcta
      781 gcgtttcata gacacctccg gctggatttt caatcaatat acttggtaca agatccgggg
      841 tttgatccca aaattcaaat gctacaccac ttagtgtgcg gctagcatat atctgcaatc
      901 taccaccttg attaatttgt ttcgcgatcc aagtgccagc ttgaagcatc agcggctctt
      961 ctatccttaa tttctcttcc aattggtgat aatagtcaaa ataacataaa ctcatttatc
     1021 tctcccgcct agctatactt gacatgaata gaaacagctg cctaatctaa ggaagtacat
     1081 aaaaaaacag tagttagaca gtaacacatg tggtctttgc tgtttcaaac acaaaaaagg
     1141 cattgaacga atacgcccaa taccttgaca tagcaaataa aacaacgtgc agaccatgga
     1201 aaatatctac ataggggtag aattttcacc ctagattagg tacaataaat taggtcgatg
     1261 cactgcattg actatggcaa aatgatcggt cagggtgtat gtgtgtgact ggtcattccc
     1321 tgattcttag cgccaactaa gggtcaggga gccttttttt attcagctgt ttctatctga
     1381 atttattgta agtaatctct tatccaagtg caagcaagaa aatacaaaa
//
LOCUS       DAZKNM010000297         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000297 DAZKNM010000000
VERSION     DAZKNM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.305"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8M9_002838"
     CDS             complement(150..1337)
                     /locus_tag="ACG8M9_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0379527.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKNM010000298         1408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000298 DAZKNM010000000
VERSION     DAZKNM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.306"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1279
                     /locus_tag="ACG8M9_002839"
     CDS             <1..1279
                     /locus_tag="ACG8M9_002839"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0379528.1"
                     /translation="TIDTKAYLLRMFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLT
                     FFVELFTDVSAAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMF
                     SFEGIKGYGYVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGL
                     FVSYVAHQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLK
                     KYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKF
                     EYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILD
                     NAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLG
                     LSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            1294..>1408
                     /locus_tag="ACG8M9_002840"
     CDS             1294..>1408
                     /locus_tag="ACG8M9_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFQ0379529.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLK"
BASE COUNT          510 a          159 c          218 g          521 t
ORIGIN      
        1 tactatcgat acaaaagctt atctattaag aatgttcgtg ggaaactttt tcttttttct
       61 cttattttat atgactaata ctgatcttaa caaaagaaaa tatctttttg tatgtttatt
      121 gataccactc actttttttg ttgaattatt tactgatgtt tccgcagcta tacctataat
      181 cggaagttat tttatattaa aggaaaagaa gaaaactaat atagtgcttt taaataaatt
      241 actattatgt atgcttattg atgatttcag atctattatt acttccatgg ttatgacgta
      301 tatgttttca tttgaaggga taaaagggta tggttatgta gtattccaga taatgcttga
      361 ttctttgttt ttgctgatgt ttatttggtt atatagaaaa ttagctataa ataggattgt
      421 tgaacagtcc agttctaaac ttactgctat attcatgatt tatttactta caattggttt
      481 atttgtttct tatgttgctc atcaatatca agtatttgat cattttgtgt ttggtgtctt
      541 aatattttta atcattcaaa tgtttgttgt tctattttta tttattagaa taactattag
      601 gcaacgagaa caatatgaac agcaattgaa aagaaaagaa ttggtttatc taaaaaaata
      661 tactgattca ctggaaaaag atcaagaaaa atttgccaga tttagacatg actataaaaa
      721 tttattgctg agtttaaaag aaatcgctaa tcaaaaccat gattcgatat tggaaaaaga
      781 aataaaagaa ttagagatct attctaattc ttatctaacc aataagtttg aatatcgtta
      841 tttaaaaaat ataaaaaatg aatatttgaa aagtttgttg atagcgaagc tataccaagc
      901 aaataacaat aagatttgtt gtaaattcga atgtccaagt actattattg aagttcctgt
      961 tccaatattt gactgtatta gggtgcttgg aattattctc gataatgcaa ttgaagcatc
     1021 tatagagagt caagatagag aactgtctct agctatttac cgagatgata aacagataga
     1081 aatttcgata tcgaatagtt gtagagagat actactctct atagatactc taatgaaaaa
     1141 aggaataaca acaaaaaagg gacatcaagg gttaggttta tctagtatag aggaaatcaa
     1201 taaaaagaat cccaatatgt ttgtgaacta tcaaaacgat ccgtatcaat ttactactca
     1261 agttattttg ttgttttgag ggaaggagga tttatgagtt atccaataat attatgtgaa
     1321 gatcaaatta tacaattaaa tcagttagaa cgaattattg ataatttcat tctttttcac
     1381 gataaagtat ttaagatagt attgaaaa
//
LOCUS       DAZKNM010000299         1406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000299 DAZKNM010000000
VERSION     DAZKNM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.307"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1386
                     /locus_tag="ACG8M9_002841"
     CDS             <1..1386
                     /locus_tag="ACG8M9_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379530.1"
                     /translation="ICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
BASE COUNT          495 a          202 c          251 g          458 t
ORIGIN      
        1 atttgtacga ttctgattag tttttattat ggagaaaagt tttttaaaaa gatagatgct
       61 gcttttttat ttagttgttt atatactttt gcaacttatc gaacagttga tatctattat
      121 cgatccgcaa ttgcagaagc aatcgctctt acgattatcc cagctgtttt atattatgct
      181 tatggattga tattcaatag agaaaaaaaa gtcatcgctt tatctgtcag tataagtcta
      241 cttgtgtata ctcatgtctt aactacacta ttgtgtagtg cattgattgc ttgtatgatt
      301 atagttcgtt tgtttttaag caagcaaata aaaaacgata taaaatttgt ggtcgtgaat
      361 ttagtaaagg ctgttttttt gacttgtgca ttaactagct atttttggta tcctatgttg
      421 cagcagatga attatcaaga aatacacagt cccttcaaaa cagatttgca gctagaagca
      481 ctttctattt cagatagtat cattcaagcg ttaaataacg atattacaac atacactatg
      541 gggttattag gattggttgc gttgattttt cctttattca tactgaaaga aatggagaaa
      601 aaagaaaaaa tcatctatgt tggcgtagtt tctacatggg tatgcgtaac taatttattt
      661 ccatggtttt tgtttcaaaa aacaccgata agcttactcc agtttccttg gcgtatctta
      721 ggtatccaaa tctttttttc ttcaatgatc ataacgcttg tttttatgaa aaagactatg
      781 aataaaaaat attcatggtt aatcattggc gctactgtgg tgtttctact cgtcacgtct
      841 atagcagcaa aagaaaatta cgcaaaagtt atccaatcaa aacacggtca tattgttatc
      901 aatgaagaaa cactcccacg ttatacaaca tcagatatgg gaggattata cgattacgcc
      961 ccaacagaag cgataaaaga aaggcctcac ttagaaaaac atgaagtaaa agtaggagaa
     1021 tcgtgggaaa aagggaagta taaagctgca gataataaca ttacttacac agtggcaagc
     1081 aacaaaaagc aaaaagtgac agtaccagta tatgcttatt taggtacaca ggtaaaagta
     1141 aacggtaaag tcgtaaatga ctcttataaa aaacatggct tagtgaatgt ggatgtaaat
     1201 gctggagaaa ataagataga aattacaaca aaatatactc caactgctct catggcttta
     1261 tggatcagta ttctgactta ttgtttagtg gtgtattcaa gttttaaacg atttgcacct
     1321 aataagttga aagattctcg atatgtgact aaaaaggaga taaacaagaa gaatgaaaat
     1381 aaataaaaaa gtagtaatat tgagtg
//
LOCUS       DAZKNM010000300         1369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000300 DAZKNM010000000
VERSION     DAZKNM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.308"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG8M9_002842"
     CDS             <1..1125
                     /locus_tag="ACG8M9_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0379531.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT          506 a          214 c          263 g          386 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaa
//
LOCUS       DAZKNM010000301         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000301 DAZKNM010000000
VERSION     DAZKNM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.309"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG8M9_002843"
     CDS             82..>1364
                     /locus_tag="ACG8M9_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0379532.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZKNM010000302         1353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000302 DAZKNM010000000
VERSION     DAZKNM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.310"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACG8M9_002844"
     CDS             complement(136..426)
                     /locus_tag="ACG8M9_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379533.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACG8M9_002845"
     CDS             complement(544..804)
                     /locus_tag="ACG8M9_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379534.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..>1353)
                     /locus_tag="ACG8M9_002846"
     CDS             complement(941..>1353)
                     /locus_tag="ACG8M9_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0379535.1"
                     /translation="ANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYKL
                     TADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPNV
                     DEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          502 a          226 c          205 g          420 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg cca
//
LOCUS       DAZKNM010000303         1349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000303 DAZKNM010000000
VERSION     DAZKNM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.311"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..1209
                     /locus_tag="ACG8M9_002847"
     CDS             40..1209
                     /locus_tag="ACG8M9_002847"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0379536.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          489 a          246 c          222 g          392 t
ORIGIN      
        1 ttctttctgt aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct
       61 aaaaaaagaa aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc
      121 tctacaggaa ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc
      181 catgctcaat cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa
      241 aaaatcgatc aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat
      301 aatcgcatca tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat
      361 gatccttctc tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt
      421 ttaaaacaag tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca
      481 caaattgctg gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg
      541 caccgaactg cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt
      601 cagcacttaa aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata
      661 ttgactggaa ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat
      721 atcatcgaac ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc
      781 aatcatgcac tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa
      841 tctgaaacag agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat
      901 ttaaatacgc tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc
      961 tcattatggc gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat
     1021 aattatatcc tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa
     1081 gattcacagg atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg
     1141 cctactgctg atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca
     1201 aatagctgat atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa
     1261 aaagtactcg actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta
     1321 cagactttaa ttccaaataa acagaacag
//
LOCUS       DAZKNM010000304         1326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000304 DAZKNM010000000
VERSION     DAZKNM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.312"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..284)
                     /locus_tag="ACG8M9_002848"
                     /pseudo
     CDS             complement(111..284)
                     /locus_tag="ACG8M9_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8M9_002849"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8M9_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          475 a          217 c          179 g          455 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaa
//
LOCUS       DAZKNM010000305         1319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000305 DAZKNM010000000
VERSION     DAZKNM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.313"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..283
                     /locus_tag="ACG8M9_002850"
     CDS             <1..283
                     /locus_tag="ACG8M9_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0379537.1"
                     /translation="ENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFD
                     GQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            288..1235
                     /locus_tag="ACG8M9_002851"
     CDS             288..1235
                     /locus_tag="ACG8M9_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0379538.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          438 a          189 c          271 g          421 t
ORIGIN      
        1 tgaaaatgaa gttactatag ttaataaatc aagatacaca tctaggcaac agacacctga
       61 agtttttgaa atgaatgcct caatgtattc ttttaaacct gaatttctaa aaaaaagtaa
      121 ttatattttt gatgggcaat gtgggataat aaaaatgaga gattatctaa ttttagatat
      181 tgatagtgaa gaagatttta aatggttaga atttttgttt cctaaatttt tagaagattc
      241 ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg agctgttatc
      301 ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta ggagaagaac
      361 atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt gatatgacga
      421 aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa gagatcatga
      481 aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg gcagattcag
      541 ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg attttagaat
      601 tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca gcggctgttt
      661 atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca ttgacgcctt
      721 atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat ttatatgatg
      781 tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat ccgaattctc
      841 catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc gtaggagaag
      901 caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt tatatagatg
      961 atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg aaacaattca
     1021 atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc aatgaaattc
     1081 tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc catgactctc
     1141 tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt attttaagtg
     1201 gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa gattagaggg
     1261 taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa tctgggata
//
LOCUS       DAZKNM010000306         1281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000306 DAZKNM010000000
VERSION     DAZKNM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.316"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1190
                     /locus_tag="ACG8M9_002852"
     CDS             150..1190
                     /locus_tag="ACG8M9_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0379539.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          518 a          181 c          206 g          376 t
ORIGIN      
        1 agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat
       61 gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa
      121 taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata
      181 gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca
      241 aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg
      301 caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg
      361 aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg
      421 aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata
      481 atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg
      541 aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac
      601 ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca
      661 taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata
      721 atatattaga cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat
      781 tgttgttaga caattttgag acaattatga caaacgacag cattgctacg tttgtccctg
      841 aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg
      901 tccagactat tcataatgca aagaaaaaag ctgaaataga aagtgatatt tcgatagttt
      961 ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa
     1021 aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct
     1081 atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt
     1141 tgaattatgc aagctcccca agcactatta cgaaagactg gttagagtga aactttactg
     1201 acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta ttattctaat
     1261 tatattacga atattacaaa a
//
LOCUS       DAZKNM010000307         1265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000307 DAZKNM010000000
VERSION     DAZKNM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.317"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1154
                     /gene="dnaN"
                     /locus_tag="ACG8M9_002853"
     CDS             24..1154
                     /gene="dnaN"
                     /locus_tag="ACG8M9_002853"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0379540.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT          412 a          226 c          228 g          399 t
ORIGIN      
        1 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
       61 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
      121 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
      181 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
      241 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
      301 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
      361 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
      421 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
      481 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
      541 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
      601 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
      661 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
      721 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
      781 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
      841 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
      901 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
      961 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
     1021 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
     1081 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
     1141 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
     1201 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
     1261 tttaa
//
LOCUS       DAZKNM010000308         1251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000308 DAZKNM010000000
VERSION     DAZKNM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.318"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..739)
                     /locus_tag="ACG8M9_002854"
     CDS             complement(<1..739)
                     /locus_tag="ACG8M9_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0379541.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(729..>1251)
                     /locus_tag="ACG8M9_002855"
     CDS             complement(729..>1251)
                     /locus_tag="ACG8M9_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0379542.1"
                     /translation="INRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKG
                     IAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSV
                     QPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMK
                     RDAGAVGGTLYAT"
BASE COUNT          394 a          235 c          148 g          474 t
ORIGIN      
        1 atacttttct ccaccaccta ggcattattt caaatgaatg aatatttttc gtccatttaa
       61 tttcattttt tccaggatat aaaaatggaa aatctaaaaa taaggtgacc tgatcctgat
      121 gttctaaatt accatctaat tttcgataag cctcttctaa tttagaatct ataaataatt
      181 ctctgtccaa cgatttcaaa tcatatttat ccttattaat ataaaaagaa gcatctccag
      241 aaccactcaa tttaataagc ggttttgaag aatacttctc tgggttataa atttcaaaag
      301 tttctgtttg tcgaatagta aatctaccat tgtaattttc cttaaatggt cgaatactca
      361 ctgtaaattc aaaagggaca atatttcctg tttttcttgt tcctttaaat tcaggtcctt
      421 ctattactac agcttgataa atatattgtt catcatagta aagaataaaa tcactataag
      481 acgacatatc taaccattca gtgattcgat cttcccactc ctgcaccata tcaattgaag
      541 gtgctttata gtaacactca atctttctag tcacattttt gtaatagcct ttatcaataa
      601 tgatagaatc atttccttct cgctctctca attcaattac tcgactagct gaaacagaag
      661 caggcctgtt ttgaatatac acattaaatt cagaagaata atgttgatta ataaaaaatt
      721 gtcctctctt aagttgcata taatgttcct cctactgcac cagcatctcg tttcatttgt
      781 cttgtcaatt ctgttttaat ttttgtggta atcttccgaa ccattgaatc aggtaaatct
      841 ccataaacat ttaaatgtaa atgaatttct tttgtttcac tagcagatat attttgcttt
      901 gtttgtgtaa ttggtaggtg acttattcca tttactacag gttgaacaga cggtgttttt
      961 actaaatttg tcatggtttg atctaactta ctttgctctt tatcaatacc aacgataata
     1021 ccttgaacaa tatttttacc aaccatatca cgcatccatc ttgatggaga atgaatatct
     1081 aaagctcctt taatccaatt tttgatatta cctgcaatac ctttgatagt atctttcaaa
     1141 gcattccact tttcttttac accatttata agtccatcaa tgatgttctt gccaatttca
     1201 aataaattga cttcccttaa cgaattaaag atttctttta cccgattaat a
//
LOCUS       DAZKNM010000309         1248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000309 DAZKNM010000000
VERSION     DAZKNM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.319"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1002
                     /locus_tag="ACG8M9_002856"
     CDS             <1..1002
                     /locus_tag="ACG8M9_002856"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0379543.1"
                     /translation="KGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQ
                     QELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMG
                     TQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSC
                     VRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFG
                     ETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIER
                     SYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1014..>1248
                     /locus_tag="ACG8M9_002857"
     CDS             1014..>1248
                     /locus_tag="ACG8M9_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0379544.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELS"
BASE COUNT          495 a          163 c          234 g          356 t
ORIGIN      
        1 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
       61 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
      121 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
      181 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
      241 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
      301 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
      361 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
      421 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
      481 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
      541 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
      601 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
      661 tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata
      721 tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca
      781 ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat
      841 gataaaatgt atatcattcc accacactat catgctactg acgaactgta caaagattat
      901 gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa
      961 tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat
     1021 cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt
     1081 tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta
     1141 aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta
     1201 aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctc
//
LOCUS       DAZKNM010000310         1239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000310 DAZKNM010000000
VERSION     DAZKNM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.320"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..729
                     /locus_tag="ACG8M9_002858"
     CDS             <1..729
                     /locus_tag="ACG8M9_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFQ0379545.1"
                     /translation="WLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLTLL
                     PLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNVTP
                     YVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYISED
                     LWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDMY
                     KKYTGKTGCLDSTYAADIEERREQ"
     gene            726..1206
                     /locus_tag="ACG8M9_002859"
                     /pseudo
     CDS             726..1206
                     /locus_tag="ACG8M9_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          427 a          182 c          280 g          350 t
ORIGIN      
        1 tggcttaatc aatttgggaa tataataatg atgcaaaagc cggaggatat ggaattattc
       61 ccacctgaag aaaagggatt ttcctatctt atgctatttg atgattacaa taaaattgat
      121 cttaccttat tgcccttgga agagttagat aattacctaa agggcgataa attaataaag
      181 gttctaattg ataaagattg tagaattaaa agggacatag ttccgactga tatagattat
      241 catgtaagaa agccaagcgc aagggagtat gatgattgct gcaatgaatt ttggaatgta
      301 acaccttatg ttattaaagg attgtgccgt aaggaaattt tatttgctat tgatcatttt
      361 aatcagattg ttcgccatga gctgctgaga atgatatcat ggaaggtcgg catcgaaaca
      421 ggctttaaat taagtgtagg caagaactat aagtttattg aaaggtatat atccgaggat
      481 ttgtgggaga aacttttgtc cacctaccgg atggattcct atgaaaacat atgggaagca
      541 ttatttctat gccatcaatt gttcagggcg gtatccggtg aggtggcgga aaggcttcat
      601 tatgcctatc cggagtatga taggaatata acaaaatata ccagggacat gtataaaaaa
      661 tacactggta aaaccggctg cctggatagc acatatgccg ctgatataga agagaggcgg
      721 gaacagtgat tacagaaatg aaagcagggc acctgaaaga tatcgataaa cccagcgaac
      781 catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg acctttacag
      841 aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat gaagaggatg
      901 aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat cttttatata
      961 gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat
     1021 atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac
     1081 aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat
     1141 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
     1201 ttttagaatg caaggaacag tgaattggag ttcgtcttg
//
LOCUS       DAZKNM010000311         1239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000311 DAZKNM010000000
VERSION     DAZKNM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.321"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..356
                     /locus_tag="ACG8M9_002860"
     CDS             96..356
                     /locus_tag="ACG8M9_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379546.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(718..957)
                     /locus_tag="ACG8M9_002861"
     CDS             complement(718..957)
                     /locus_tag="ACG8M9_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379547.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          439 a          169 c          224 g          407 t
ORIGIN      
        1 tttttataaa atcacaagtg ttattgattt tcataatcat attttaatgg tattatcatt
       61 ttaaataatg gaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag
      121 gaaaaaaata tatttgccaa cctattggct tgaaacaaaa agtggttgga gaagtaatca
      181 gtaaattaga aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg
      241 aaatattaga aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa
      301 caacagaata tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat
      361 ttaaataaag tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg
      421 gcagtgattg ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg
      481 taacgaggcc tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc
      541 ggatagacaa actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa
      601 aactgatatc aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca
      661 tttagttagg agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta
      721 attcagagtt tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc
      781 agaggtagaa acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat
      841 tgttgaatcg atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt
      901 cccacaagca gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat
      961 gtacacactc cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa
     1021 tgcaaaacta tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg
     1081 gtgatatccc agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct
     1141 aaacgctttt tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg
     1201 taattttttt gggtatttaa atagttgcta ggaggatag
//
LOCUS       DAZKNM010000312         1236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000312 DAZKNM010000000
VERSION     DAZKNM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.322"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..702
                     /locus_tag="ACG8M9_002862"
     CDS             <1..702
                     /locus_tag="ACG8M9_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0379548.1"
                     /translation="DEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTG
                     MADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSL
                     GYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGV
                     KRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVK
                     RPISADSVLLEEDVE"
     gene            699..>1236
                     /gene="neuC"
                     /locus_tag="ACG8M9_002863"
     CDS             699..>1236
                     /gene="neuC"
                     /locus_tag="ACG8M9_002863"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0379549.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTI"
BASE COUNT          412 a          163 c          270 g          391 t
ORIGIN      
        1 gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca
       61 ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta
      121 ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat
      181 agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat
      241 ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat
      301 tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa
      361 cattttacct tggataagga actacctggt ccagatcata aagcttcttt agatcctcag
      421 gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt
      481 aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc
      541 actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct
      601 ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca
      661 ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa
      721 gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata
      781 attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta
      841 aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag
      901 agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata
      961 tatttgaaca aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg
     1021 cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag
     1081 tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt
     1141 ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag
     1201 tttttcccgt cggagctcct agaatagata ctattt
//
LOCUS       DAZKNM010000313         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000313 DAZKNM010000000
VERSION     DAZKNM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.323"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8M9_002864"
     CDS             414..947
                     /locus_tag="ACG8M9_002864"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0379550.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKNM010000314         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000314 DAZKNM010000000
VERSION     DAZKNM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.324"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..744
                     /gene="aac(6')"
                     /locus_tag="ACG8M9_002865"
     CDS             196..744
                     /gene="aac(6')"
                     /locus_tag="ACG8M9_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0379551.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(992..1111)
                     /locus_tag="ACG8M9_002866"
     CDS             complement(992..1111)
                     /locus_tag="ACG8M9_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0379552.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT          421 a          177 c          241 g          376 t
ORIGIN      
        1 ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta
       61 taattttttt attttataag taagataaat aatgaaagaa tagagatact gattggtagc
      121 taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa
      181 caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat
      241 cagctttctg atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa
      301 gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag
      361 ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca
      421 ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta
      481 gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac
      541 catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc
      601 atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta
      661 ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt
      721 attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag
      781 aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc
      841 cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg
      901 agtagtaaga aaaattttgt gaaaatggct ttctacattt ttgcataaat ttggcttaat
      961 accgagaaac tggcttttga acaccgttta ttcagataag ggactgtgac aagacttttg
     1021 tcacagtttc tttttgttta ttctttttta tctttgtatg gcaataatag acaatataac
     1081 gaaaattatt taatggcttt tggaagacca tggaaaacct tgtacaaagc tattttttct
     1141 gaaaaattcc ttgaaacctc acgaaaaaat cattatttta tgaaaaagac aaaaaaaagt
     1201 gttgattttt taata
//
LOCUS       DAZKNM010000315         1208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000315 DAZKNM010000000
VERSION     DAZKNM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.325"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8M9_002867"
     CDS             <1..459
                     /locus_tag="ACG8M9_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379553.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8M9_002868"
     CDS             473..904
                     /locus_tag="ACG8M9_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379554.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACG8M9_002869"
     CDS             906..1043
                     /locus_tag="ACG8M9_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0379555.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT          442 a          175 c          254 g          337 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
     1201 aaagtaag
//
LOCUS       DAZKNM010000316         1196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000316 DAZKNM010000000
VERSION     DAZKNM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.326"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..988
                     /locus_tag="ACG8M9_002870"
     CDS             35..988
                     /locus_tag="ACG8M9_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0379556.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            960..>1196
                     /gene="prgO"
                     /locus_tag="ACG8M9_002871"
     CDS             960..>1196
                     /gene="prgO"
                     /locus_tag="ACG8M9_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0379557.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLND"
BASE COUNT          428 a          186 c          227 g          355 t
ORIGIN      
        1 taaatatatt tagtatagga aattggaggg aactatggaa aacttacgga ctgcatttaa
       61 aacatcagat aaactaggaa aagttgtaat gcttacaggt agaaaaggcg gcattggtaa
      121 gactacagac aatgatttat tagcgattgt ttctagtcaa ctttttgaaa aagacgtgtt
      181 gctaattgat tatgaccaac aaagaaatac tacttccaac attggatcaa cgtatcaaat
      241 tactagcttt gatcgatcca tgtctgcagc aatcaaaaaa ggggactggg tttcaggtat
      301 tactcaagta agtccacatt tatatattat ggctggctct cctggatctg aagaacttaa
      361 tgaatacttg tccgaaaaat atcctgatcg tcgtaagaga tcattggcct tcattaaacc
      421 tttagaagaa ttgagaaaaa atttcgatta tatctttatc gactgcccac catcaacaga
      481 taatgtggtt cgtgcattct taactgctgc agattatatt attgcaatgc aagaattaaa
      541 acggtacgca atggaaggaa cagaagattt tattaataaa gttttagttc cgattgtcac
      601 gaattttgaa gagtctcatc ttcaaatcat tggaattttg cctgttcttt tctcagtgcg
      661 acgttcctct caacacgcaa actatcaaaa aacaattgat aagtatggtg aatcaaacat
      721 ttttaaaagc attgttaaag gctcggatcg gttagaaatg tatggtgaga atggtatcca
      781 attaaatgat tatgtagatc gaagatggtg ggctattttt gcagacatct ttacagaatt
      841 agaagaaaga attgaatact atgaacaaca tggtgatatt gaaggatttg attatacacc
      901 taaatatgct gactcaatgg ccaatcatat cctacctctt ggaaaggaga ttaaactaaa
      961 tggcattatt accaacgaac aaagataaga agaaaattgg tgctgaagat atttcaatta
     1021 aagatgatat taatgtcgag ttaggggaac gaaagtacac tgcgaaagaa agaaaaccag
     1081 ttcaagtaga tcctcccgta ctaaaaacca ttcgggatct tagttatgca aaagatattc
     1141 ctatgtatga aatagtcaaa cttgcaatgg acgcctatgt tgatacttta aacgac
//
LOCUS       DAZKNM010000317         1196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000317 DAZKNM010000000
VERSION     DAZKNM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.327"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..872
                     /locus_tag="ACG8M9_002872"
     CDS             24..872
                     /locus_tag="ACG8M9_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0379558.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          404 a          204 c          201 g          387 t
ORIGIN      
        1 tattatgaaa atgaggaatt ctcatgagcg tctccataag actacaaatc aaaacaatgt
       61 acaacgactt cagcccaaaa gaacaggcta tcgccgatta tattttagaa aatccaagca
      121 aagtaagtca tagtcctatt agcaatttag caagtgaact gggcattgct gactctactt
      181 tttttcaatt tacaaaaaaa cttggttata atggatttaa agattttaaa atggccatgt
      241 tgatccaaga aaatgatcta tctgctattt ctatccacga aaatattcag aaaacagata
      301 ctgagctcac tatggcgcaa aaggtgttcg agtctagtat ttcaacttta aatgacacaa
      361 aaaagttgtt acaacaaaag gatctaaaaa aagcggctac tattatcaat gaatctaaac
      421 gtgtctattt tttcggtgtt ggcggttcag agattgtagc aactgacgct tatcataaat
      481 ttttgcgttc ccctatcgca acttctcaca gcacggatta ccatattcaa ttgatggaag
      541 cttctttatt gacggaaaaa gactgtgctg ttttgatttc tcacacaggg caatcaaaag
      601 aaacaatcca tattgctgaa accgtaagaa aaactggagc aaaaacaatc gttattacta
      661 gccaagctaa ttcttccctt gctaaacttg gcgatgtcgt ttttatttct atttctgaag
      721 aaacagaatt ccgatctgaa gctttagctt cccgtatttc tcaactaagc attttggact
      781 ctctatatgt gattttgatg ttttacaacg aaaaagaatc acgtcgttct atcgctaaag
      841 ttcgacatac tatttctgaa atcaaagaat agcaccataa cagaaaaagc gaaaaatcca
      901 taaaacaaaa aaagccacta ccaaatattt ggtagtggct tttttggttc tgcaagggtg
      961 gtacagaaca tcaatagtcg gtgggttaga acgtatgtca tcgcatcttt ggagaaggtt
     1021 gtattggtat tattcgagtt acttgttaga tgcgataaca aagttagtat acagtagtaa
     1081 aaaatttatc ttttcttttt tttattattt ttgtttctta ttttctaaaa aattaaaaaa
     1141 aattttggtg aattaagtat ttccttgata acactggctt ttggcctcat tttagg
//
LOCUS       DAZKNM010000318         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000318 DAZKNM010000000
VERSION     DAZKNM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.328"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8M9_002873"
     CDS             85..624
                     /locus_tag="ACG8M9_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379559.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          443 a          156 c          188 g          406 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tat
//
LOCUS       DAZKNM010000319         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000319 DAZKNM010000000
VERSION     DAZKNM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.329"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8M9_002874"
     CDS             <1..1076
                     /locus_tag="ACG8M9_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0379560.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZKNM010000320         1190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000320 DAZKNM010000000
VERSION     DAZKNM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.330"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8M9_002875"
     CDS             <1..186
                     /locus_tag="ACG8M9_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0379561.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG8M9_002876"
     CDS             329..553
                     /locus_tag="ACG8M9_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379562.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..>1190
                     /locus_tag="ACG8M9_002877"
     CDS             568..>1190
                     /locus_tag="ACG8M9_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0379563.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLN"
BASE COUNT          405 a          180 c          255 g          350 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca
//
LOCUS       DAZKNM010000321         1175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000321 DAZKNM010000000
VERSION     DAZKNM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.331"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..740
                     /gene="erm(B)"
                     /locus_tag="ACG8M9_002878"
     CDS             3..740
                     /gene="erm(B)"
                     /locus_tag="ACG8M9_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0379564.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            910..1170
                     /locus_tag="ACG8M9_002879"
                     /pseudo
     CDS             910..1170
                     /locus_tag="ACG8M9_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          448 a          196 c          198 g          333 t
ORIGIN      
        1 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
       61 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
      121 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
      181 acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa
      241 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
      301 atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccgtgcgt
      361 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
      421 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
      481 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
      541 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
      601 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
      661 taaacaattt aagtaccatt acttatgagc aagtattgtc tatttttaat agttatctat
      721 tatttaacgg gaggaaataa ttctatgagt cgctttttta aatttggaaa gttacacgtt
      781 actaaaggga atggagataa attattagat atactactga cagcttccaa gaagctaaag
      841 aggtccctag cgcctacggg gaatttgtat cgataagggg tacaaattcc cactaagcgc
      901 tcgggacccc ttgtaggaaa atgtcctaag tgtggcaaca atattgtatt aaaaaaatcg
      961 ttttatggtt gttcaaatta tcctgaatgt aagtttactt tagctgaaca ttttagaaag
     1021 aaaaaactaa ccaaaacgaa tgtaaaagaa ttactggagg gaaaagaaac cctggtaaaa
     1081 ggaatcaaaa acaaagagaa aaagccctac aatgccgttg taaaaattgg ggaaaaggga
     1141 tatattgatt ttatatcttt ctcaaaataa acata
//
LOCUS       DAZKNM010000322         1173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000322 DAZKNM010000000
VERSION     DAZKNM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.332"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..930
                     /locus_tag="ACG8M9_002880"
     CDS             <1..930
                     /locus_tag="ACG8M9_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0379565.1"
                     /translation="DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDV
                     STAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEG
                     IKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKP
                     QVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVT
                     ATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKT
                     GESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1064..>1173
                     /locus_tag="ACG8M9_002881"
     CDS             1064..>1173
                     /locus_tag="ACG8M9_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HFQ0379566.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKS"
BASE COUNT          475 a          160 c          242 g          296 t
ORIGIN      
        1 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
       61 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      121 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      181 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      241 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      301 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      361 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      421 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      481 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      541 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
      601 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
      661 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
      721 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
      781 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
      841 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
      901 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
      961 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1021 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1081 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1141 ttatgaagtt attctaatcg ataaaaaatc aga
//
LOCUS       DAZKNM010000323         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000323 DAZKNM010000000
VERSION     DAZKNM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.333"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..480
                     /locus_tag="ACG8M9_002882"
                     /pseudo
     CDS             166..480
                     /locus_tag="ACG8M9_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(785..>1170)
                     /locus_tag="ACG8M9_002883"
     CDS             complement(785..>1170)
                     /locus_tag="ACG8M9_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0379567.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          421 a          163 c          212 g          374 t
ORIGIN      
        1 taatcttctt tatagagaat tgggctattt ttaatggcaa agaggggaag aagatgtttc
       61 aaaacaaagg acaacgctat gttgtaaaag gagtaaagga atcttactgg aatcaaaaat
      121 ctttattgag aaaattatct ttgaatttaa gcacaaagaa atgaaggtac actagatagc
      181 aatgaagata tctttgtcat taaacgtcgc tattttacac aagtaagtga tgatttgatt
      241 catgtctttt ccaatgagtg gtttgtttct gagaaaaaat tacatgcttc taatctgcaa
      301 tatatgccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
      361 gaaggatata aagccaaaca tccagaagcg aaaccattta aatacccaca agaaatgaag
      421 cgagcatggc gtaaaatgtt agatgatgag ctgattcccc ttgaaaatga attgaggtaa
      481 acgagaagaa ttcaataaac tggatgataa aaatataata tgactataaa ttggttacag
      541 cttcaaccta taagttgtaa taaattagcc taaaagtaat ttattaatga tgaggtgaat
      601 ttgattggta caacaagata tatcacttag agagggagaa gcagcactac aaactacttt
      661 tcaataagta ggaattatag aaattttact caaaaaatct tcagtaaaca atttggctaa
      721 ataagattca gttgttatac gagaaggtta cggttcttct taactaagct tagtatgttt
      781 ttattcaagg catgattttc tctatgtgat cattcagttt ctgtaagagt tctaataatt
      841 cttttttgga gtacttgtaa ttgctttcta cccaccgatt gagaacggat agacaacctt
      901 ggacgtggta cgtagtaaag aatctcattt cttctgtgat aatcgttttc ttgtcttcat
      961 atagccatat gttcagatag ttttcttcaa taacttgttt aactttcttc tgaaacgaaa
     1021 tatcaccatg gttgaggata aataagcgat ataaaaaagt atttgcgtaa acatagtcaa
     1081 tcaagcgagt gtatagttcc tcatagctat gggctggatc ataagagaga atttcattta
     1141 gatcccgaaa aaagttgctt tctaattgat
//
LOCUS       DAZKNM010000324         1140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000324 DAZKNM010000000
VERSION     DAZKNM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.334"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..923
                     /locus_tag="ACG8M9_002884"
     CDS             <1..923
                     /locus_tag="ACG8M9_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFQ0379568.1"
                     /translation="NREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQAPTGYELDA
                     TPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQS
                     GLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRL
                     TPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLV
                     ETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKT
                     GETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          417 a          197 c          282 g          244 t
ORIGIN      
        1 agaaccgaga aggagaaacg ctacaaaccg gtctaactac aggagaagat ggaaaactgg
       61 caattgacgg tttagcacca ggagcatatc agctggtaga aactcaagcg ccaacggggt
      121 atgaattaga tgcgacaccg atcgagtttg agatcgaaag aagtcagaca gcggtggtag
      181 aattgacgaa agcaaaccga cttacaccag gcggtgtcgt gttgacaaag atcgatgacc
      241 aaagcgggga agtcctgcaa ggagctgttt ttgagttaca agatgccaat ggaaccgttc
      301 tgcagtcagg tctaaccaca ggagcagatg gaaaactggc gattgacggc ttagcaccag
      361 gagtgtatca gctggtagaa acccaagcgc caacggggta tgaattggat gcgacaccaa
      421 tcgagtttga gatcgaaaga agtcagacag cggtggtaga attgacgaaa gaaaatcgac
      481 ttacaccagg cggtgtcgtg ttgacaaaga tcgatgacca aagcggagaa gtcctgcaag
      541 gagctgtttt tgagttacaa gatgccaatg gaaccgttct gcagtcaggt ctaaccacag
      601 gagcagatgg aaaactggcg atagatggct tagcaccagg agcatatcag ctggtagaaa
      661 ctcaagcgcc aacggggtat gaattggatg cgacaccagt aactttcaag atcgaaaaag
      721 aacaagaagt ggcgatcttt ttaacaaaag agaatagaaa agttgctgat agctcagata
      781 taagaggaaa caatccaaca agcagtggaa ataagcatct tccaaaaact ggagaaacca
      841 tcattagcca gtttatattg tcaatattgg gtgtgctact tgttttcatc agtataaaat
      901 ttagaaagaa acgaaatata tgagctaaaa taaaaaattg aataagcata gtttttaaaa
      961 tcaactagta cccatcaatt gaatggaaac taccgatttt agaaggggta tacaaacaaa
     1021 tattagtatc ggagtagaga aggttgaacc caaccagtaa ataagtaaag acaattttgt
     1081 gctgcgttga gaagtgaaaa ataaaccttt gtagtaatac tagttaataa aaaatataca
//
LOCUS       DAZKNM010000325         1114 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000325 DAZKNM010000000
VERSION     DAZKNM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.335"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..781
                     /locus_tag="ACG8M9_002885"
     CDS             <1..781
                     /locus_tag="ACG8M9_002885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0379569.1"
                     /translation="NIPVIIHLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEK
                     WKKIIKEIEPNCKTVVVENSIVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKN
                     IAEQYPEKKFIFEIAGAGDEEISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVM
                     ILPSYNEGLPISILEAISYGMPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILK
                     INDKDKYVKFSRCSKDLAITKFNDGNFFDRILNLYQKGSKE"
BASE COUNT          419 a          116 c          234 g          345 t
ORIGIN      
        1 aaatattcct gtaatcatcc atcttcatgg atcggaattt aaaaaatggt atgatagtgt
       61 ttctgtaaaa aaacaaaatc agattcgctt gctgttaaga gagtctgatt ccacgattgt
      121 acttggagaa aaatggaaga agataattaa agaaattgag ccaaattgta aaactgttgt
      181 tgtagaaaat tctattgtag ttccagaata taccataaag tggaattcaa aaatgtgccg
      241 atttctgttt atgggagttt tgataaagcg aaaaggtgta cacgatttgg tagaagcagt
      301 taaaaacatt gcagaacaat atccagaaaa aaagtttatt tttgagattg ctggagcagg
      361 tgatgaagaa atatcgctca aagaattgat agaagaatat aatttgcaat cgtattttga
      421 atttacaggt tgggttgtgg gagaaaaaaa ggaggagtgc tatagaagat caaatgtgat
      481 gattttaccg tcctataacg agggattgcc tatatctatt ctagaggcga tttcttatgg
      541 aatgcctgta attgctacag atgtaggaga tatttcttca gcagtacgtg atggagaaaa
      601 tggatatttg tttcaacctg gtgacgtatc agcgttgaca tctgcaatac taaaaatcaa
      661 tgataaagat aaatacgtaa aattttcacg ttgttcgaag gatttggcaa taacgaaatt
      721 taacgatgga aatttctttg atagaatttt aaatctgtat caaaaaggga gcaaggagtg
      781 atgacaagga aaataaaaaa tgtttcgatt atgtaatgaa cttattaagt gaataaaata
      841 gagagaaaaa atattgaaat agacgatgta aatttttatc cgtgtaagaa ttcaaatgaa
      901 tagtagtgtt ttagtgcttg taatagagca agagtacaag cacggtacat tatataaaaa
      961 cattaatgat atttctataa ataaatagat tttgtattct ggatgatgaa gaggaagata
     1021 ttatctgata acttacccta aactttgcag ggggtggtta taagttgtgt atatgtggca
     1081 gtaatgaatg agtgaaataa tcatattaag aaat
//
LOCUS       DAZKNM010000326         1113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000326 DAZKNM010000000
VERSION     DAZKNM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.337"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1082)
                     /locus_tag="ACG8M9_002886"
     CDS             complement(<1..1082)
                     /locus_tag="ACG8M9_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFQ0379570.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGE"
BASE COUNT          398 a          213 c          150 g          352 t
ORIGIN      
        1 tcttcaccgc tatatgcttt aattgtttct attcctttaa gactttctat gatactagaa
       61 tttacagctg caccggctgc catttcttcc ctgttcgatt tttcatagct tttcacgaaa
      121 gcgaatattg caactacata aaaaggcaaa gagcatagag tgattaaaaa caacgtacta
      181 ttctgaatag ctaacgtaac cccaacaagc aacaccatac caatatctaa aaatattgat
      241 aacgtagcac ttgctaaagc atcaataatc ttactggcat caagaaaacg agaaataatt
      301 tcccctgatt ttctcgtagc aaaaaaattc attggtaaag ttaacacatg tttgaaatac
      361 tgaagcataa ttgccatact cattcgctgt cctaaaataa taagaagata actacgacta
      421 tattcaaaca atacccgaaa taaatagaca gcgatcaagc caatggaaat tatatttaaa
      481 gttgaacgtg cttgattagg gatgaaaaaa tcaagaatac cttgaaaata atatgagctt
      541 cctatgccaa aaaacgtaat gaaaatcgat gctaaaacga tgtggaatac taatcctttt
      601 tgtttccaaa ttataggcaa aaaagaactt aatccagcta ctttttcttt aataggaata
      661 taagtttctc taggcatagg taataaaaga atacctgtcc attctttttg aaacgcttca
      721 atcgtttttc tattttttcc attagcggga tctgcaatcc atagtacgtt tcccttcgtt
      781 ttataaatta caacgtagtg catatagctg ttatcaacca atatatgtgc aattaatggt
      841 aagataagat ctctttcttt ccagatctca ctatctgctt gaatagccat acaatcaaaa
      901 gacaattttt caaatgtctt tttcaatcca aatgctgaag taccctctaa atcagtccca
      961 gataggtccc gtaatttatg aatcgggata tcagttttaa aatattttaa aatcatcgaa
     1021 aggcaagcaa caccacaatc tttttcatct tgttgctgca caaaaggaaa tgattttatc
     1081 acttatctaa cacaactcct tctaaaaaat ttt
//
LOCUS       DAZKNM010000327         1112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000327 DAZKNM010000000
VERSION     DAZKNM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.338"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG8M9_002887"
     CDS             154..417
                     /locus_tag="ACG8M9_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0379571.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="ACG8M9_002888"
     CDS             417..689
                     /locus_tag="ACG8M9_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0379572.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="ACG8M9_002889"
     CDS             766..1095
                     /locus_tag="ACG8M9_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0379573.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          423 a          141 c          220 g          328 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa ac
//
LOCUS       DAZKNM010000328         1108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000328 DAZKNM010000000
VERSION     DAZKNM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.339"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(123..>1108)
                     /locus_tag="ACG8M9_002890"
     CDS             complement(123..>1108)
                     /locus_tag="ACG8M9_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0379574.1"
                     /translation="LISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIIL
                     SLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFR
                     KSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKT
                     PLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKV
                     DLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNY
                     AFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          430 a          187 c          135 g          356 t
ORIGIN      
        1 tagcgttttc aaattcagaa taaataaata aatctccttt tgattctcgg taaaaatttt
       61 gataaacatt taatatatta atcaaatgta aaggagtcca agcaatgaaa gctgtcttca
      121 tattaattct ccttttttaa atacaagtaa gaaatgcttc ccaaataata ccagaaaaca
      181 attgaaatga catcgcgctt attgaaaagt acatgagcct ccaccatttc atctataaaa
      241 atagatagta taaaaattaa aacaatacca agaaacgcat aattcacttt acttctaaaa
      301 aatgttttga gtaaactaaa gatatttaac acatagaaag catagataca tataaaagca
      361 attataccac ttgatacaag tacttgaaga aatccattat gcatgtttcc atgtgctctt
      421 tttagttcat tcacatctgt agtatcaaga tctacttttt tcaaaaattt tgcatcgcta
      481 ttcccataga aatcggtctc tccataacca aataacaaag ttggtcttat aactttaagc
      541 gcagctttcc aaatcgtaaa acgaccagct gaaatttcgg agttatcact agaatgctgt
      601 aatattactt ctttttctgg aagtggagtt ttatctgatt catttgactc tataacttct
      661 acttttctat caatcgatac aggaattgtt gctaaaaaat ccttagcata attagtacca
      721 aaagaaaaat ataccaatag aactaataat atgcttacca tccatttgat ttttacaggt
      781 agtacttctc taatgctctt tctaaatttt gaattggcaa acaacaaaag aaataatact
      841 aaaaaagaac aaattactac ctgggcacct ctagacgaag aaagtgtaat ataaaatatt
      901 tgtacgataa aattcatcat ataaaaaaca cgaatcgttt ttgttatttt tgaaattaat
      961 acactaatta aacttaaaat aatagaagta tatccaaaca tagctccaac atttggactt
     1021 gtatagacac caaataaccg atgttcaata aaaccttgtc ttgcaagtaa tcctggcgtt
     1081 cctgttggga catgataaga aatcaaaa
//
LOCUS       DAZKNM010000329         1106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000329 DAZKNM010000000
VERSION     DAZKNM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.340"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1014)
                     /locus_tag="ACG8M9_002891"
     CDS             complement(<1..1014)
                     /locus_tag="ACG8M9_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0379575.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGV"
BASE COUNT          374 a          199 c          124 g          409 t
ORIGIN      
        1 aaccccaata atggattggt ttagtaaaaa atagttaaat attcgttgtt taattggatt
       61 ttgtttatat gggtatgagt gaatacaggc aatcaactca tcgttcataa gtatcttaga
      121 caaataggag tttaaatcaa attctttatt aaaactagca tatctaactg ccttttgaat
      181 acaagtacga cagcgtccca acaaaagtct ccttgttttg tttacaaata tctcattttc
      241 taatatatag cttaaatcgc cttcaatttt tttaactaat tcaatattac aatcgaactc
      301 attatctcta aaattatgtg tcaatgaatt attattatta catctataat aatagataca
      361 atctttaata aaacttgtta tttttgaatg ttgcaaatat gaaatatgga atatgatatc
      421 ttcagatata taatcccttt ctgacggaaa cttgatattg tgctgttgaa ttatctcaga
      481 tgaatacagc gcatgccaaa ctgacattgg caatataaaa tcgactttat aactcggctc
      541 agtgccaatc attccaagca acactttttc aattatagcg ttatttgtaa atatcgtact
      601 atcatatctt gctggctttg aaataatatt attatcatca taatattcat aatatccagc
      661 ataacatgtg tctgcattac tcctttttat accatttaac attgcttcaa tataattagc
      721 tgcaacaaaa tcatcagaat caataaaagc aacatatttt ccttttacat gttcaagtcc
      781 tgtatttctt gccaatccca agccagcatt atttttatga ataatacgaa tccttgaatc
      841 ctttaatgca tagtcttcac atattttatt agatgaatcc ttagatccat catccacaat
      901 tataatatct aggttcttat atgtttgatt acacactgat tccaaacaac ttcctacata
      961 cttttcaaca ttatatacag gtatgattac actaattaga tcactcgaat tcatccttct
     1021 atcctttcac tcacgttttc tcaattagtc aatgacggca tcaacgattt tcttaaattc
     1081 tctgaaaaaa atagactctg atcttg
//
LOCUS       DAZKNM010000330         1083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000330 DAZKNM010000000
VERSION     DAZKNM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.341"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..926)
                     /gene="thiD"
                     /locus_tag="ACG8M9_002892"
     CDS             complement(111..926)
                     /gene="thiD"
                     /locus_tag="ACG8M9_002892"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0379576.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          289 a          236 c          173 g          385 t
ORIGIN      
        1 agcttgcatc tgcggtaata agtccaacca agttaaaaac ataagaagct gtttttactt
       61 ggctgttctt attacttgat gcagggcaac tttttcacaa cttctttctt ttactctaat
      121 tcaccagctg cccaatgatt gatggggcca tatttgtgcc ctacatttat ttcttcacga
      181 attgcacggt ttacatagtc ttttgccaat cggatcgctt gttcaactgt ttttcctttc
      241 gctaactcag ctgcaataca tgcagatagc gaatcgccag ttccgttgat ccggtcggta
      301 ggataaaatg gtgcatttaa ccaaaatgat tttcctgatt cgaacaatac gtagtcattg
      361 actgtttctt gctgcgcatt tttagcatga tgtcctttga tcataacgtt ttttgcaccc
      421 atatcttgca gttcttttgc tgcttgcaca aaatcttctt cagctgctaa tgtccgtcca
      481 gtcagctttt ctgcttcata aaaattaggt gtcagtactt cggccaaagg gacaagcttt
      541 tctttcaagc tttgcaaagc agattcttct agtaaaagat ttccatgttt cgttacgatt
      601 acgggatcca cgactaatgg tccaaaatca tatttttggt aattttttac aactgtctca
      661 atcaactttg aatctgctag catacctgtt ttagctgcac gtatttgaaa gtccttcgca
      721 atcagttcaa attctttgtc gataaaatct gttggcaatg tcacacttgc tccgattccg
      781 taagaattac ctgcaacaca tgcggtcatg acagaaacac cgtaaacatt tcttgtaaag
      841 aatgtatgca tatctgcttg catccctgcg cttccatcgc tatctgatcc tgcgatcgtc
      901 attgcttgta catactgatc ttccatcaaa tcgattcctc catttttata aaaagatttt
      961 tcataatact gttactgttt ctatcatatg tttttcaaag tatccctcag taaatatacc
     1021 actacctttt cttggtaatc aaatttattt gaaaaaacat ttgcaataaa tcagaaaata
     1081 gtg
//
LOCUS       DAZKNM010000331         1078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000331 DAZKNM010000000
VERSION     DAZKNM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.342"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..202
                     /locus_tag="ACG8M9_002893"
     CDS             <1..202
                     /locus_tag="ACG8M9_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0379577.1"
                     /translation="AIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEV
                     NEIVSEINNFLNEESSDERKQI"
     gene            183..917
                     /locus_tag="ACG8M9_002894"
     CDS             183..917
                     /locus_tag="ACG8M9_002894"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0379578.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            950..>1078
                     /locus_tag="ACG8M9_002895"
     CDS             950..>1078
                     /locus_tag="ACG8M9_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFQ0379579.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDY"
BASE COUNT          383 a          149 c          240 g          306 t
ORIGIN      
        1 agctataaaa ctgcttgaaa cttttgaata taaacctgaa aaatatgccg aaagagttaa
       61 tcatattttt gaagtactcg gtctttctct ttttgaatgc tacgatatga ccgagaaact
      121 ttataaagaa gtgaatgaaa ttgtatcgga gataaataac tttttaaacg aggagagttc
      181 agatgaaaga aaacaaatat gatgataata tattttttca aaaatacagt caaatgagtc
      241 gctcgcagaa aggactggct ggtgcgggag aatgggagac tttgaaaaag atgctacctg
      301 attttaaggg taagcgtgtg cttgatttag gatgcggcta tggatggcac tgtatatatg
      361 cgatggaaaa cggtgcttcc tctgtagtag gtgttgatat ttctcataaa atgctcgaag
      421 tagcaaaagg aaaaacccat tttccacaga ttgaatatga atgctgtgcc atagaagatg
      481 tggatttccc agaggagagc tttgatgtaa tactaagttc gcttgcgttt cattatgtag
      541 cagactatga gaatttaata aaaaagatat ataggatgct gaaggctggt ggcaatttag
      601 tttttacagt tgaacatcct gtttttactg ctcatggaac acaagactgg tattataacg
      661 aaaaaggaga aatactgcat ttcccggtgg acaattatta ttatgagggc aaacggacag
      721 ctatgttttt ggaagaaaag gttacaaaat atcatagaac actgaccaca tatctaaata
      781 cactgctttc aaatagtttt ataataaatc agattgtgga gccacagccg ccagagaaca
      841 tgatggatat tccggggatg gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg
      901 caaaaaagaa gatgtaataa tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga
      961 aaaagaaatg atggatttag tactttcttt agcagaacag gatgaacgta ttcgaattgt
     1021 gacccttgag gggtcacgcg caaatattaa tatacctaaa gatgaatttc aggattat
//
LOCUS       DAZKNM010000332         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000332 DAZKNM010000000
VERSION     DAZKNM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.343"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8M9_002896"
     rRNA            <1..>1073
                     /locus_tag="ACG8M9_002896"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKNM010000333         1069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000333 DAZKNM010000000
VERSION     DAZKNM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.344"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8M9_002897"
     CDS             <1..969
                     /locus_tag="ACG8M9_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0379580.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZKNM010000334         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000334 DAZKNM010000000
VERSION     DAZKNM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.345"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACG8M9_002898"
     CDS             complement(37..>1062)
                     /locus_tag="ACG8M9_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0379581.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZKNM010000335         1010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000335 DAZKNM010000000
VERSION     DAZKNM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.346"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..989
                     /locus_tag="ACG8M9_002899"
     CDS             45..989
                     /locus_tag="ACG8M9_002899"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0379582.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          345 a          157 c          222 g          286 t
ORIGIN      
        1 tttcatgaaa gtaacaaata gttatgaaaa ggagctgttt attcatgaaa aaaacaagtc
       61 gtaaagtagt aatcgtcggt acaggttttg ttggaacaag tatcgcttat gcaatgatca
      121 accaaggaat ctctaatgaa ttagtattga tcgatgtgaa tcaagaaaaa gcagaagggg
      181 aagcattgga ccttttagat ggtatggctt ggggcgatga aaatgtggct gtatggtcag
      241 gtggctatga agaatgtaaa gatgctgata tcgttgttat cacagctggt atcaaccaaa
      301 aacctggtca atctcgttta gatttagtta aaacaaatgc gtcaatcatg cgccaaatcg
      361 taaaagaaat catgggatca ggattcgacg gtattatcgt tgttgcttca aacccagtag
      421 atattttgac atatatcgct tggaacgaat ctggtctgcc aacatcacgt gttatcggta
      481 ctggaacaac attggataca actcgtttcc gtaaagaaat cgcattgaaa ttaaaagttg
      541 acccacgcag cgtccacggc tatatcttag gtgaacatgg tgattcagaa gttgctgcat
      601 ggtcacatac aactgtcggt ggtaaaccag tgtttgaaat tgtagaaaaa gaccatcgta
      661 ttgcccaaga tgaattagat gtgattgctg ataaagtccg taatgctgct tatgagatca
      721 ttgatcgtaa aaaagcaact tactatggta ttggtatgag tactgcacgt atcgtaaaag
      781 ctatcttgaa taacgaacaa gctgtattgc ctgtatctgc ttatttaact ggtgaatacg
      841 acgaaaaaga tatctttaca ggtgtgccat caatcgttga cgaaaatggt gttcgtgaag
      901 ttgttgaatt atcaatcaac gaagaagaaa aagcaatgtt cagcaaatca acaagtgctt
      961 taagagaagt attgaacact gtattataag aaacaatgaa tatgaactaa
//
LOCUS       DAZKNM010000336         1008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000336 DAZKNM010000000
VERSION     DAZKNM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.347"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..830
                     /locus_tag="ACG8M9_002900"
     CDS             90..830
                     /locus_tag="ACG8M9_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379583.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          372 a          180 c          196 g          260 t
ORIGIN      
        1 tttcttaaaa taatcagcgc attgctactg aaaacaatga atctgtgcta ttattgagaa
       61 aagttccatt tttgagaagg atggtttgaa tgaaagaaat cagacgaaaa catttccgtt
      121 ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa cgacaagaag cgagtattat
      181 ttgacggaca agaagatctt ttgactgaac agaatcagtc cccgtttgaa cagatgaaag
      241 aatttcattt cacgaacacg aacaccgaga aaatccgtcg caagcgtcgt gcaacaaatg
      301 gattgaagtc agctccgcgt aaggaaaatt tggaaaagca taaaggaaaa ttaccggatt
      361 ataccggcca ttaccaatca gaaatcacat cgacaggacg aaaaaaactt tttggtgatt
      421 cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc caagcatact acacgaaacg
      481 catctgttga cacgaaggtg cgggagcgtt cttattttgt tcctaaatat gtacccgcat
      541 cgattatccc agaccctaga gaaagcaaaa tttctgagga tcatttaatc gaatcgatga
      601 aaaaaacgga tgactcttat ctgttgttcg atacagaacc gacgccgtat caagaaaaaa
      661 gaaatggtga gccgtctgta cgaacattca atcgaacaaa aacgttagag gaaacaaagc
      721 agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt agaacgctca ttacaaggat
      781 tgatcgatga tcaaggaaac tctttgggag aaaatagcta ttttaaataa gtgatataca
      841 aatttatatt cggctacagt tggaaaggaa acgctgcggc gtttccttat tttatactct
      901 atccgaaagt ttatatcgta tcctcaaatt tttttgagtt taagcgaatt ttttctccca
      961 ataagtaaga aaataatggc aaaataaaaa aacaaactat tttcgcag
//
LOCUS       DAZKNM010000337          990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000337 DAZKNM010000000
VERSION     DAZKNM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.348"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG8M9_002901"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG8M9_002901"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0379584.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          347 a          128 c          176 g          339 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt
//
LOCUS       DAZKNM010000338          979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000338 DAZKNM010000000
VERSION     DAZKNM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.349"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(239..718)
                     /gene="rlmH"
                     /locus_tag="ACG8M9_002902"
     CDS             complement(239..718)
                     /gene="rlmH"
                     /locus_tag="ACG8M9_002902"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0379585.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT          256 a          200 c          157 g          366 t
ORIGIN      
        1 cttaaaaaag cactaaaatt ataagagagg gaggtaatgg tctgaacacc aagaattgtt
       61 ccaacatcga attgcttaac taatgagttc aaagcttaat tatgtggctc ttatatttac
      121 cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt tcataaaaaa aacagccata
      181 tccgctttct tggatatgac tatttactac tactttacta tctttcattc ttcttgtctc
      241 acttatgata aggcgccccc gcattgatcc ggaaggcacg gtagatttgc tcgacaagga
      301 tcagacgcat caattgatgt ggatacgtca tttttccaaa agagatctga gcattgcttc
      361 ttttcatgac ggcttcgctg agtcctaaag agccgccgat aacaaaaaca aggtggcttt
      421 ttccttggat acctaattgg tcgatttgtt tagcaaaaga ttcacttgta ggatttttcc
      481 cttcaattgc taaggcatag acgtattctt gttctttgat tttcgctaag atccgttccc
      541 cttctttttc ttttacttgt ctcatttgcg cttcgcttaa attctccggg gctttttcgt
      601 caggaacttc gatcaattcg atttttgcat aggcattcag ccgtttgaca tactcattga
      661 taccttggat caggtatttt tcttttaatt ttcctactga gataattttg atattcatgt
      721 tttctctcct ttggtcttca tgctcacttt gtttattata atcaattttt tcgtctatat
      781 gtttattttg gctatttttg ttaaaaagat ttccccaaca gttatgcaca acatccacag
      841 gtttatccac agaaaacagt gttattttac cttatttcat cacattcatt aggcaaacat
      901 acgttttata agaatttatc cactttatac acaggctgtg gatatgtttt atcccttatt
      961 tttccaatcc tatccgcgt
//
LOCUS       DAZKNM010000339          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000339 DAZKNM010000000
VERSION     DAZKNM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.350"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..512
                     /locus_tag="ACG8M9_002903"
     CDS             105..512
                     /locus_tag="ACG8M9_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379586.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            526..927
                     /locus_tag="ACG8M9_002904"
     CDS             526..927
                     /locus_tag="ACG8M9_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379587.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          356 a          124 c          167 g          314 t
ORIGIN      
        1 ctaatatttc cgttgacaaa gctttgttac tattgttaaa ctaaattgac tagcaaaagt
       61 gaaacataaa tttattaaat aaatgtaaaa attaggagaa atcaatgtct tatagagtac
      121 aattaataat ttcagaagat gtagaaagtc agcaatttgg cactaatgtt attaataaag
      181 taattaatcc agctctttct attaatgcgc ctttaattcc tactgcttta tcattttctg
      241 taacagctgt ggtgtctgag atagaggata cagaaaagat aaaaatagta gaaattgaag
      301 ttttaaacaa gaatgaaaaa caaatttttt ctacgggtga agtatcggta agcttgcctc
      361 ctcaagttaa cgatataaac tttaatataa acgccagaaa tgtcttggtt gaagaagcag
      421 gagagcatta tgctgttgtt aaatttaatg gaactgagat tggaaggcaa atctttgata
      481 tcaaggtcaa caaaccagtg gaaaaaaatt aaggagacga tacggatgct tgatatagta
      541 ccatcaaata cacatcggag tggtagctta ataaattttg ctgcgatttt tgcttgcgca
      601 tcggtaattc tagcatctcc agttgttgtg gatgcaagtt ctactccgaa aactaaaaat
      661 gataatcaac ttgtaattac tacaaaaaat gagattaaca caatttcaag taatgatggc
      721 aaaatttttg atgtgataaa tacggtttta aaaagaaaag ctgaaatcaa tataaaatat
      781 gatgaagatt taaacttgta tttctttgtt atcaaaacaa catctgaatt gttttcctca
      841 gattacgatg ttttggatac tctcgataat gtattatctg actataaata tatggggaaa
      901 tctgtagtag caactttggg ggaataaaat gtttgacttt aatgaatatt tatctatttc
      961 c
//
LOCUS       DAZKNM010000340          947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000340 DAZKNM010000000
VERSION     DAZKNM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.351"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..908
                     /locus_tag="ACG8M9_002905"
     CDS             99..908
                     /locus_tag="ACG8M9_002905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0379588.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          313 a          168 c          188 g          278 t
ORIGIN      
        1 taactgtagt ttttgttagg aatagctgat tgctcttgtt aattacctaa aatatgctag
       61 actaaaacaa atgctagttg aggaaggaag gacccttatt gaataaaaaa ttaattgcca
      121 tcgatctgga cggaactacc ctcaatcaag attcgatgat tacatctaaa acaaaagaga
      181 ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat tgctacagga cgcccattcc
      241 gaatgagcca tcagttttac caacaattag aattaacaac accgatggtt aattttaacg
      301 gcgcattagt acacatccct aatcagcact gggatggtga aagagaaacg ttgatcaatc
      361 gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct aaatttagat tttatcgctg
      421 ctgaaaatcg tgatactttt tttattgaca gttttgattt ctttgatgaa aaaatttttg
      481 cttcttctcg tccaggagag aagaatttgc tttctcctaa aaatttgacg acaaatccta
      541 catctttgct tgtccgaaca gacaaacgtt ttgcaggagc tgtatctgct gaattaacac
      601 gtcaatttgg cagttatgtc gatgttcgga catggggtgg acctactgcc atcttagaga
      661 ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga gattgctaac tacctgtcta
      721 tcgatcaaaa agacgtcatt gcctttggtg acgagcataa tgatttggaa ttgttggatt
      781 atgcaggttg gggagtagct atggctaacg gtactgacca actcaaaggc attgcaaatg
      841 atgtcactcc tttgagcaat caggaagacg gtttagctgt ttatctggaa aaactactta
      901 aactataata gagaattaga ttccacaaaa aagaggttgt gacaatt
//
LOCUS       DAZKNM010000341          918 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000341 DAZKNM010000000
VERSION     DAZKNM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.352"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..918
                     /locus_tag="ACG8M9_002906"
                     /pseudo
     CDS             286..918
                     /locus_tag="ACG8M9_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          308 a          172 c          173 g          265 t
ORIGIN      
        1 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
       61 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      121 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      181 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      241 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      301 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      361 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      421 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      481 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      541 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      601 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      661 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      721 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      781 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      841 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      901 tcagacttat tttttacc
//
LOCUS       DAZKNM010000342          915 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000342 DAZKNM010000000
VERSION     DAZKNM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.353"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..396
                     /gene="spx"
                     /locus_tag="ACG8M9_002907"
     CDS             <1..396
                     /gene="spx"
                     /locus_tag="ACG8M9_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFQ0379589.1"
                     /translation="TSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAAT
                     EGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNE
                     DEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            410..766
                     /locus_tag="ACG8M9_002908"
     CDS             410..766
                     /locus_tag="ACG8M9_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0379590.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          305 a          153 c          178 g          279 t
ORIGIN      
        1 acttcgccta gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt
       61 gaatttgaag agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagatc
      121 ttggcagcga ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa
      181 aagctgggca tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa
      241 tatccttcat tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt
      301 aatgaagatg agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg
      361 atctatttac aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa
      421 agtctatgga tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca
      481 tcaattgcct tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga
      541 agaattatgc agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc
      601 ttttcacaac attgcgattg acctttcctg tgtaggaaaa aaacaactga tttttttatg
      661 cacacaacat accaatctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat
      721 tggatacaat gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt
      781 aatcaagtca ctgagcattt catacagttg taacagtcga acacttagca tcaagcgtgt
      841 tatgtgttct ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc
      901 aaaattcaaa tctag
//
LOCUS       DAZKNM010000343          910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000343 DAZKNM010000000
VERSION     DAZKNM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.354"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8M9_002909"
     CDS             <1..355
                     /locus_tag="ACG8M9_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379591.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8M9_002910"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8M9_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAZKNM010000344          899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000344 DAZKNM010000000
VERSION     DAZKNM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.355"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8M9_002911"
     CDS             <1..878
                     /locus_tag="ACG8M9_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0379592.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          288 a          201 c          183 g          227 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattat
//
LOCUS       DAZKNM010000345          898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000345 DAZKNM010000000
VERSION     DAZKNM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.356"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG8M9_002912"
     CDS             256..879
                     /locus_tag="ACG8M9_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0379593.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          332 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaaga
//
LOCUS       DAZKNM010000346          887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000346 DAZKNM010000000
VERSION     DAZKNM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.357"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..445)
                     /locus_tag="ACG8M9_002913"
     CDS             complement(<1..445)
                     /locus_tag="ACG8M9_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379594.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKG"
     gene            complement(462..866)
                     /gene="tnpA"
                     /locus_tag="ACG8M9_002914"
     CDS             complement(462..866)
                     /gene="tnpA"
                     /locus_tag="ACG8M9_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0379595.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          146 c          139 g          339 t
ORIGIN      
        1 tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta ggaactttga
       61 tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta taactctgaa
      121 caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga aaaaaattct
      181 tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca atttctttca
      241 aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt ttagaagaat
      301 catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg tagaccttac
      361 ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga tagattctga
      421 atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg attgtttcct
      481 tgattttgga tatacttctt aatcacatca attggtgcgc caccagttgt cagaagacaa
      541 aaacttttag accagaacat ttctttccaa agaaattgtt tgactcttgg aaagtcacgt
      601 tttatcaatc ttgagctggc acttttatag gcattgatga attttgtcaa ttctgtttta
      661 ggttgagctt tgaacataat atgaacgtgg tctttatcat gattccactc cactaaagta
      721 atgtgatacg attctgaaat tctttcaaaa gtagttttgg cataatcaga tatttcatca
      781 tcaatcactt gtctacgata tttcgttact aaaacaaggt ggtaataaag aagaaatact
      841 gaatgattat ttgtatctaa ttccatgtta tatcaactca tttcttg
//
LOCUS       DAZKNM010000347          858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000347 DAZKNM010000000
VERSION     DAZKNM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.358"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..724
                     /locus_tag="ACG8M9_002915"
     CDS             <1..724
                     /locus_tag="ACG8M9_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0379596.1"
                     /translation="EQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFN
                     LSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVS
                     LPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVS
                     ETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFK
                     KYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          311 a          102 c          153 g          292 t
ORIGIN      
        1 cgagcaaatc aaaatagata atctgttatc acaaaattac gatccagaaa tatatctgtt
       61 tctttatgaa aataaaatac tggaatatgt taaaaatgga gatattcgaa atttagaaaa
      121 catggttttt aatctgagta atggaataat tccaagtgtt agtggagata cgattcggtc
      181 tgaaaaaaat tattctatca ttgtttttga aaagttagca caaacgtcta taacattagg
      241 aatggatata atagaagcgt atcaaagtag agatgctttg atacaagaaa acgaattagc
      301 tgtatcgtta cctgaggtct taaaagtgag ggattctggg attgtttatt atactaagga
      361 aatcggaaaa actaagatag agcatctttc tcccttaatt tcttctgtcg ttcaattcat
      421 cggcttaaat atctataaaa gaattactgt aaaggaaata gctaattatt tttctgtaag
      481 tgaaactaaa ttaagagaaa gctttaagaa tgaaatgcat attacgattt ataattatat
      541 ctctaagcga aaaatttcta cggctaaaat aatgttgaaa tcaaaccata ctatcagtga
      601 agtttcttta ggtttaggat tttcagattc ttcacacttt tcgagagtat tcaaaaagta
      661 cgcaggtgta tctcctaagc aatatcaatt aggtttagtg gacaatttca acaacattag
      721 ctgagaggtt ttattttatt atgagactat atcatttacg tagtttggag gagttgagga
      781 aattggattt taacaaaaaa tttttttggg aaatttctga tttgagttgt ttcgtagaat
      841 ctgagcgtct aagtaatg
//
LOCUS       DAZKNM010000348          855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000348 DAZKNM010000000
VERSION     DAZKNM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.359"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..670
                     /locus_tag="ACG8M9_002916"
     CDS             <1..670
                     /locus_tag="ACG8M9_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0379597.1"
                     /translation="HQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIIS
                     KENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVD
                     CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAG
                     IESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAK
                     EKMA"
     gene            712..855
                     /locus_tag="ACG8M9_002917"
                     /pseudo
     CDS             712..855
                     /locus_tag="ACG8M9_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          309 a          157 c          151 g          238 t
ORIGIN      
        1 ccatcaagtt ttattaaaag aagggatttc agtatctctt aaacatgtcc aaaagctaat
       61 gaaacaatta aatttaaggt cgattgtagt taaaaaatat agacctcaaa ggtctaataa
      121 accgatcatt tcaaaagaga atctcttaaa tcaggacttt tctactgaaa cgatctgtga
      181 gaaatgggca gctgacatta cgtacattcc tactaagaaa aatggttggt gttacttatc
      241 ttcaatcatg gatttacaca cgaaaaaaat tattagttat acattttcaa aacgaatgac
      301 tgtggattgt gtcattcaaa cattgaataa agcaaaaata cattatcaca ttccagaagg
      361 aatgattcta cacactgact tgggcagcca atacacagca agagaagtgg aacaatggct
      421 taaaaccaac aaaataaggc attcttatag tagaaaagga acaccttatg ataatgctgg
      481 aatcgaatct ttccatgcct cattgaaaaa agaagaagtc tacacgacta gctactcaga
      541 ttttgaagaa gcaaatcgag cgctatttag ctacattgaa ggattttata accgaaatcg
      601 aatccatagc tcgattcact atctaacccc acaggaattt gaagaattag caaaagaaaa
      661 aatggcttaa ccgtctctac taaaatgtgt ccaagatatt gactcaaatc catgatttac
      721 aaaggaactc ttctgtacaa gccagaagag tgttcccatt gtctttgtgt cgtcccctct
      781 aggatcattc gttgggggac gacaaccgtt cggttgttac tgaatgatgc gtctgagtat
      841 cgcacttatc tggag
//
LOCUS       DAZKNM010000349          844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000349 DAZKNM010000000
VERSION     DAZKNM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.360"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..>844
                     /locus_tag="ACG8M9_002918"
     CDS             56..>844
                     /locus_tag="ACG8M9_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0379598.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIK"
BASE COUNT          335 a          120 c          149 g          240 t
ORIGIN      
        1 tatgaaaata gagataataa ttgtacactt aagctgtatt caggaggtga agataatggg
       61 catcgaattt agcgaacggc aaatgaaaat actatacttt ttgattgaaa atgagcacac
      121 aacttacaaa gaattatcag aacaattata tgtatctacg aaaacaatta gcaaagatat
      181 tttaattttg aaagaaataa tccaaaaaat gccagatgta caaatgttgg ttcaacctaa
      241 aaaaggcgtt tcgcttattg gaaaaaaaga aacacttcga aatttattat ccagtcataa
      301 aatcaatatc gcagactctc cggaggaccg tctaacttat gtgtttgcta aattattgaa
      361 tactaatgat tctctaaaga ttcaaaatct tgctgacgaa ttatacgtga gcaggtcgac
      421 ggtagaaagc acaatgaaag atgttagaaa aagactaaac aagcatggga tgtccttagt
      481 ttcagatcga aatggcgtgc gcatcaaagc tgatgaaaaa agtaaaagaa ggttgatgtc
      541 agaagttgtg aactattatt ggggtggctt tacagcatct aacagtaaaa cagatctctt
      601 agacttgaat atcaatttca ttggtgaagc gaataatttc ttaaataagg acatattgaa
      661 aaaagtagcc gaattgctta atcgatacat gaataagaca caattgaaat ttacagatta
      721 tgaatttcaa actttagcta tccaccttgc tattgcgata gaaaggattg aaacaagcaa
      781 tttcttatca cgtagtaata attctgaaga agaaatctca aagaatacac ttattttaat
      841 caaa
//
LOCUS       DAZKNM010000350          837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000350 DAZKNM010000000
VERSION     DAZKNM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.361"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..>837
                     /locus_tag="ACG8M9_002919"
     CDS             45..>837
                     /locus_tag="ACG8M9_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HFQ0379599.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEK"
BASE COUNT          293 a          143 c          151 g          250 t
ORIGIN      
        1 aaacaaccta ttttatagga tttataggaa aggagaacag ctgaatgaat atcccttttg
       61 ttgtagaaac tgtgcttcat gacggcttgt taaagtacaa atttaaaaat agtaaaattc
      121 gctcaatcac taccaagcca ggtaaaagca aaggggctat ttttgcgtat cgctcaaaat
      181 caagcatgat tggcggtcgt ggtgttgttc tgacttccga ggaagcgatt caagaaaatc
      241 aagatacatt tacacattgg acacccaacg tttatcgtta tggaacgtat gcagacgaaa
      301 accgttcata cacgaaagga cattctgaaa acaatttaag acaaatcaat accttcttta
      361 ttgattttga tattcacacg gcaaaagaaa ctatttcagc aagcgatatt ttaacaaccg
      421 ctattgattt aggttttatg cctactatga ttatcaaatc tgataaaggt tatcaagcat
      481 attttgtttt agaaacgcca gtctatgtga cttcaaaatc agaatttaaa tctgtcaaag
      541 cagccaaaat aatttcgcaa aatatccgag aatattttgg aaagtctttg ccagttgatc
      601 taacgtgtaa tcattttggt attgctcgca taccaagaac ggacaatgta gaattttttg
      661 atcctaatta ccgttattct ttcaaagaat ggcaagattg gtctttcaaa caaacagata
      721 ataagggctt tactcgttca agtctaacgg ttttaagcgg tacagaaggc aaaaaacaag
      781 tagatgaacc ctggtttaat ctcttattgc acgaaacgaa attttcagga gaaaagg
//
LOCUS       DAZKNM010000351          832 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000351 DAZKNM010000000
VERSION     DAZKNM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.362"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            233..490
                     /locus_tag="ACG8M9_002920"
     CDS             233..490
                     /locus_tag="ACG8M9_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379600.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            504..>832
                     /locus_tag="ACG8M9_002921"
     CDS             504..>832
                     /locus_tag="ACG8M9_002921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379601.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
BASE COUNT          311 a           97 c          172 g          252 t
ORIGIN      
        1 atgcgatcaa gatttattat gtacggagtg cgtattaaaa taaaaaatga acactcatat
       61 ttattataca ccgaagaaat agaaaaaaac agactgtttt tttaagaaaa actatgtcat
      121 tctttacttg aaaaaagttg ttataggaca gaaaggataa aacaaaaacg gaaaaaatat
      181 gtcttatgaa taactataag aactattcta ttttttagga gatggatacg agatgaaaaa
      241 actaattggc tatagtgtga tgtgtattgg atttgttggt gtgggattgt ttgtatcgac
      301 tgaatctgct tctgcggctg aatggtataa tcctatctgg tataatccga acattgaatg
      361 tcaaaaaggc tatccgtatg gaccgacaaa tctgaaaaat ggaaaatgta gagtaaattg
      421 gaatggcgtt gtagatagtg taattactgg ttcaatggac tctgctctac atggctttac
      481 gggtcactag aaggaggaag aaaatgtctg aagagaaaaa agaaatcgtt gaatcattag
      541 tagctttaaa agattctctt tctaagattg aatatgttga taggcaagaa attcaaaaac
      601 taattgatga tactattatt gaaatccaag atgcgcgttg tgaaggtata aaaatttctg
      661 tagctttatc taaagtaata gaaaaaatga accgttcatt agcgtttaat ggattgaagc
      721 tagatagaca aactagtctg atatgggatc atttaaaaga cttgtatgac aagtctaaaa
      781 gaagtgaacg gactgctgta tcaatcttaa aaggcctttg gggaatgaat tc
//
LOCUS       DAZKNM010000352          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000352 DAZKNM010000000
VERSION     DAZKNM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.363"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..787
                     /locus_tag="ACG8M9_002922"
     CDS             74..787
                     /locus_tag="ACG8M9_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379602.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVTDN"
BASE COUNT          276 a          157 c          147 g          243 t
ORIGIN      
        1 agacaaggtt ttacggattc aaaaattcag gattatgcta tactggtatt aaaaaaataa
       61 tgaggtattt tatatgaaaa agttaggttt tattttattg ttggtctctt tattccttgt
      121 aggatgtacg tctcctagta aagaaaatag tgatccatct gcgaccacag acaaaagttc
      181 aatcagacaa accagttcag ctgaaaaatc aagtactcag aaatcagctt ctactgaaac
      241 gaatacatct tcaacaagca gtaaagtctc agagcccaat acttcttcta catctagtag
      301 tacagttgag acaaatattt cttcttcaac aatagacaca acctcctcac agtcttctca
      361 gtcaatagat ccgttagcag gatattctga tctacaaatt gagtacgcac gtgtttggct
      421 agctattaga ggtaatactt ttagagatac atcgggtgat ttcgaattac atgttattca
      481 ttatcctgca ggaacaccta tcgcccctta tgatgcaggg agtgcagtct atccgaaaga
      541 tgtggttatg ctcactggga aatacagtta ccagggagtt ttagtgtatt caagtaacca
      601 tgacgggaca atcactagtt atccagttcc ttcacattgg caaatgcctg cagatcaaac
      661 atcttatcca gcgttcatta gagaaacaac tcaagaaatc attgataatg catctgtagt
      721 agctattcct acaggaaatc ccaatgacgt gaaacagtta atcgatgtga cagttacaga
      781 taattagact ttctaatatg ttatacaaaa agaagctgac agc
//
LOCUS       DAZKNM010000353          814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000353 DAZKNM010000000
VERSION     DAZKNM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.366"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8M9_002923"
     CDS             134..307
                     /locus_tag="ACG8M9_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379603.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          274 a          131 c          103 g          306 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaa
//
LOCUS       DAZKNM010000354          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000354 DAZKNM010000000
VERSION     DAZKNM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.367"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8M9_002924"
     CDS             <1..672
                     /locus_tag="ACG8M9_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0379604.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKNM010000355          808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000355 DAZKNM010000000
VERSION     DAZKNM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.368"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..330)
                     /locus_tag="ACG8M9_002925"
     CDS             complement(<1..330)
                     /locus_tag="ACG8M9_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0379605.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLA"
     gene            complement(489..562)
                     /locus_tag="ACG8M9_002926"
     tRNA            complement(489..562)
                     /locus_tag="ACG8M9_002926"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(526..528),aa:Arg,seq:tct)
BASE COUNT          264 a          156 c          115 g          273 t
ORIGIN      
        1 agctagccca ccttgtttga gtaaaatcca acgatcgcta gtcaaactgc catataatgc
       61 aacaaaaaaa atagatatta atagcatact tgtgatttct ttagatgcgg aagaaagata
      121 agctccataa agtagtccca ctcctaatag tccattgtac actccttggt tcttaaacaa
      181 tgtttgaaca gattttctgc ttaactcttc tttactcatt ttaaaaaccc tacttgtcgt
      241 gtcagaatca gttctcaccg tttcgatata catgatataa aaaaattcaa ttgcaactag
      301 agtcactaaa attctagata gtatcgtcat atcttcttcc tcttttctga tgagttttat
      361 ttttttgctc cttataattg tactcgataa ggatgttttt ttattttatt tgcctttgaa
      421 aacaaacaat attggcacaa aaaacaaaga aaaaccgtta gaaacctcag tttctaacgg
      481 ttttttagcg tcacaggagg gattcgaacc cccgaccgtc cgcttagaag gcggatgctc
      541 tatccagctg agctactatg acaagaatca gcggaacatc aataattata gataattaaa
      601 ttgcttctgt caatacgaaa tagttattta attagcaaat aatgaattat tttggcccaa
      661 ctactaaatc aatcttttaa aacgagacat tctccttcag ctgttctaag gttatcaatt
      721 gaaaacagga gctacattga cttcatgcta ctcctgttta tttagatttc ctgtatcctt
      781 taattgactt acaaatatag aaactttt
//
LOCUS       DAZKNM010000356          808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000356 DAZKNM010000000
VERSION     DAZKNM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.369"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..457)
                     /locus_tag="ACG8M9_002927"
     CDS             complement(83..457)
                     /locus_tag="ACG8M9_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0379606.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(480..>808)
                     /locus_tag="ACG8M9_002928"
     CDS             complement(480..>808)
                     /locus_tag="ACG8M9_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFQ0379607.1"
                     /translation="LFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQMFDKGLQRPL
                     FLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGAIKVDKLDYSI
                     NYECGF"
BASE COUNT          253 a          165 c           90 g          300 t
ORIGIN      
        1 ttctcattaa actattgaaa tgctgatacc aaaaaatgaa gtacttttcg ttcataccaa
       61 cttagagcac ttcgttatct atttatttct tcatttggct tatctctata atctcatgat
      121 ttggtccgta gaaattaaag taacggattc cattttccca gaacggtaat tccataatat
      181 ttttatctaa taattcatat cctaaatctt gaattgaatt gaatacctca tctatgtcat
      241 ccgtatctaa tgctagatga tcaatggctc ctctacactc agtagtttta tcttcccata
      301 tttctattat cgttccattc aactcgaaaa agccgacttt acttcccaca ttcatttctt
      361 cattaatcag actaaaccct aaggattcat aaaagatttt tgaagctcca aaatcttttg
      421 ttggaattcc aatatgttgt aaaccattta atttcactta catcatccct tcaattgttt
      481 taaaatccac attcataatt tattgaatag tctagtttat ctaccttgat tgctccctcc
      541 gtaacagtca tacttaatgc tgttccgact ttatcataca ttctagtact taaagcaaca
      601 ttatcaatat ataaaacagc tatcgtatca tcaacaatta actttagttc atattctttg
      661 cctatttcca aaaatagtgg tcgttgcagc cccttatcaa acatttgagg ccaaggatag
      721 ttcggagttt ttccgaaagt aagctgatta ttaatcaatg aaatacgaaa ttcgtatcct
      781 tcttgagtat cactatctac aaataatt
//
LOCUS       DAZKNM010000357          806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000357 DAZKNM010000000
VERSION     DAZKNM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.370"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..765
                     /locus_tag="ACG8M9_002929"
     CDS             73..765
                     /locus_tag="ACG8M9_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0379608.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          356 a          129 c          143 g          178 t
ORIGIN      
        1 aaaacgcttg acattgtgtt tgagatcact tacttttagc atgtaaaagt attggattaa
       61 ggagctatgc aaatgaaaaa gaaatttatt actggtggga ttgtcgttgc atttctatgt
      121 ctaattggct acacaggcac gcggtacgca aatgaaatta ttttgtgggg tggagagaca
      181 gatattgata caatcaatga gaatctgaca cagcttgatc atacattgac agataaagag
      241 caagcgatta cagatcttac cgctcgacta gcacaaaaag aacagacatt gcaagattca
      301 aaggcagatg tccaagaata ccaaacaaaa gtaaatgaat tagaaaacca aaaaaatcaa
      361 tcggcagcag aaaaacaaaa cttagaaagt caattagtca gcaaggatgc acaactacaa
      421 agcaaacaaa atgaaatcaa tgtcaaacta gatgagatca accaaaaaaa tgatgaaatc
      481 aatcatctga atgcagattg gacagcaaga ctacaagaag cacaagcaaa actttcagaa
      541 ttacaaaata ctgtaaatgg tttgaatcaa caaatcgcaa cactgacaca agaaaaaaca
      601 aatgtagaaa ctcagttaac agatacacaa aagaaattag acgaagcaac tgctgaaaac
      661 tcaagcttga aacaatatat tcaaaagtta gaaaaagcaa aagatgaagt ggaagataca
      721 gctgataaat ctcagcaaat tgttgaagaa catttgcgta aataatcttt cttaacaagt
      781 aaacctaaat tagtaaagaa gcaaca
//
LOCUS       DAZKNM010000358          797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000358 DAZKNM010000000
VERSION     DAZKNM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.371"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>797
                     /locus_tag="ACG8M9_002930"
     CDS             <1..>797
                     /locus_tag="ACG8M9_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0379609.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIP"
BASE COUNT          270 a          139 c          153 g          235 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata atattcc
//
LOCUS       DAZKNM010000359          795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000359 DAZKNM010000000
VERSION     DAZKNM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.372"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..642
                     /locus_tag="ACG8M9_002931"
     CDS             10..642
                     /locus_tag="ACG8M9_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0379610.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          300 a           86 c          176 g          233 t
ORIGIN      
        1 gtaggtgcag tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat
       61 ggatataaaa aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca
      121 aacttactat caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa
      181 tataaaaatt tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg
      241 cgaagtgaag gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta
      301 tcggcaaagc gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag
      361 catcacattg aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac
      421 tgttcatgga aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt
      481 tatagtaact tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca
      541 aggacatgtg ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat
      601 acaagtggtg ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta
      661 aaaaaaaaga ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga
      721 aaagatgctg aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa
      781 ttattgtgga taata
//
LOCUS       DAZKNM010000360          784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000360 DAZKNM010000000
VERSION     DAZKNM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.373"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            377..>784
                     /locus_tag="ACG8M9_002932"
     CDS             377..>784
                     /locus_tag="ACG8M9_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0379611.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNL"
BASE COUNT          267 a          149 c          122 g          246 t
ORIGIN      
        1 ttataacaac tataacaaaa ccgaatgcaa aacagaagtt ctataagtta taatcatagt
       61 tgttaggtga ttcatctagt aaaaacacca gatttcaaat ttcacacaat ttaaactaga
      121 tcaatcatca accttcttca aattgttaga accctttccc gaggagctgc agcataagca
      181 aaaaaacaaa caggaagcca aatggctaag acattttttc tattagtcta gttgcttctc
      241 gtcgcaagtt taagacttta gaaagttatt tctaatgatg tttgggttgt tttgatcaat
      301 gcttatgcac agctccttac ctattattta tctcaacaga tctaacaaat taatggacta
      361 tttaaggagt ttttttatgc ttacgatcta ttcttctcgc acacatagtt accatctgat
      421 ggcaaactgg ctgaaagacc atcaaatgcc ttacagagaa cgaattttca cacctaaaca
      481 gcctttaaca aggaaagaga tattttattt attgtcatta acagaaaacg gatttgacga
      541 tctattagct aaacgctcga ttgattataa aaatttgaaa gaccaaatcg accaatgtac
      601 gacctcagag cttgtcgatc tgatcgtctc gcattttact ttgttacaac gtcccatcat
      661 tactgacgga aagaaattat tagtcggatt caatgaagat agcatgcgtc tttttatccc
      721 tgtacaatgg cgcagaagtc aactgaaatc tgtctataac gattttacat gggtaagcaa
      781 tcta
//
LOCUS       DAZKNM010000361          780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000361 DAZKNM010000000
VERSION     DAZKNM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.374"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..461
                     /locus_tag="ACG8M9_002933"
     CDS             3..461
                     /locus_tag="ACG8M9_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0379612.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            470..697
                     /locus_tag="ACG8M9_002934"
     CDS             470..697
                     /locus_tag="ACG8M9_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379613.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          255 a          122 c          142 g          261 t
ORIGIN      
        1 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
       61 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
      121 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
      181 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
      241 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
      301 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
      361 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
      421 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
      481 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
      541 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
      601 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
      661 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
      721 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
//
LOCUS       DAZKNM010000362          774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000362 DAZKNM010000000
VERSION     DAZKNM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.376"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..685
                     /locus_tag="ACG8M9_002935"
     CDS             47..685
                     /locus_tag="ACG8M9_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0379614.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          242 a          125 c          126 g          281 t
ORIGIN      
        1 gtatactgac tctatcttca aaaactgaat agtaggagtt attatcatga ataacatttt
       61 aggaaaaaaa atagaactta aaaaagggat attgggcact attgtcgttg tactcggttt
      121 gttagggttg ctatttgcgc catccattat tagcattttt tctttaacta tcactggatt
      181 tatagcattt acacttgttt ttggtaaaaa agatacgaaa aaaatgttct cgaaacctgt
      241 cgctcctgtc aaaaatatag ccaaatattt ttttatcaat gtagtaatca gtgccgcagt
      301 ctcggtattt ttgcaacaaa ttttgaaatg gggtttaaca ggcaatccaa taaatgaggc
      361 tttcagcccg ttattattta ttattttgcc tatcatgata cttggagaag aattgttctc
      421 agtgtatttt ctcgctattt tttctagtaa gttcagcatt cctgtcgcta gtttcttgtc
      481 agcaatcatc tttggattta tccattacag tacttatgat aatggaaata ttttacacac
      541 tgtagcacac atccttttga tacaaggggt ggcaagattg ttattcaacc aagctgccat
      601 taaaagcaac tcgatcatta caagttgggc agttcatgtg atatttgatt tcacagctat
      661 attgctagtc gttttattct cctaaatttt tatagaaata atacatgctc tattgctaag
      721 aaagcatact ttatttcctt taaacaaaaa gacagatgta tcaatggatt aaaa
//
LOCUS       DAZKNM010000363          741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000363 DAZKNM010000000
VERSION     DAZKNM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.378"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..701
                     /locus_tag="ACG8M9_002936"
     CDS             48..701
                     /locus_tag="ACG8M9_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0379615.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          312 a           61 c          134 g          234 t
ORIGIN      
        1 taatatgctg tataatatct agtgtatagg cttaagtgga ggtatatatg aaaatattaa
       61 ctgttgaaga tgataatatg ataagagagg gaataagtga atatctttca gaatttggat
      121 atactgttat tcaagctaaa gatggaagag aagccttgtc aaaatttaat agcgatataa
      181 atttggttat cttagacatt cagatacctt ttataaatgg tttagaagtg ttgaaagaaa
      241 ttagaaagaa aagcaattta ccaattctaa tcttgactgc atttagtgat gaagaatata
      301 aaattgatgc atttactaat ttagtagacg gatatgtaga aaagccattt tcattgccgg
      361 tcttaaaagc tagaatagat tctttaatta agaagaatta tggacattta gagaagtttg
      421 aatataagga tctatcggtt aattttaata gctatacagc taaaataaat ggtgaagaaa
      481 tagatgtaaa tgcaaaagaa ctagaagtat taaaatgttt attggataat aatggacaag
      541 tgttaacaag aatgcaaatt attgattatg tatggaaaga tagcgaagaa actccttatg
      601 atcgagtaat agatgtatat ataaaagaac ttagaaaaaa attacaatta gactgtataa
      661 ctactataag aaatgtagga tataaattgg agagaaaatg aaaaaattaa agatatttcc
      721 aaaaatgttc atacaaatat t
//
LOCUS       DAZKNM010000364          741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000364 DAZKNM010000000
VERSION     DAZKNM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.379"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..86)
                     /locus_tag="ACG8M9_002937"
     tRNA            complement(13..86)
                     /locus_tag="ACG8M9_002937"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ile,seq:cat)
BASE COUNT          264 a          113 c          127 g          237 t
ORIGIN      
        1 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
       61 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      121 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      181 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      241 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      301 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      361 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      421 gaggtgaact tttatgctga tggaaggaaa aacacaatta tggtttaagc atgttccttc
      481 aaataagttt tttaattata aactctagag aaattaattc atcctaacat aaaataattg
      541 ttcactcaaa agtttttcta gaacaattaa aacagcaggc ttcacattcg ctatacattt
      601 gtaagaagta ataattcctg tactaaaagg cataattgct ttaattattt tttgacttct
      661 cttcaaacac aagaagtagt tgtgtaataa ttgcagaaat aaggattcca ataatagata
      721 taaaatttga catggaaacg t
//
LOCUS       DAZKNM010000365          737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000365 DAZKNM010000000
VERSION     DAZKNM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.380"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..694)
                     /locus_tag="ACG8M9_002938"
     CDS             complement(146..694)
                     /locus_tag="ACG8M9_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0379616.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          240 a          160 c           75 g          262 t
ORIGIN      
        1 ataatccatt tccacctcca agcatattca atactggtga acatgcctta gaaacacgaa
       61 tatagttatg gtttaaatag atattttctt taaacattgc ttgtttaaaa gccatatgct
      121 cttgttctaa acttttcata aacttttact ctaatgcaca gctagcagca gctgctgcaa
      181 ttcctgctac aatatttacc ttagctaact ctttagctgc agcttttcct aattgttttt
      241 ctgcaatttt taataataaa tccgcagctt tttcaaaatt aaaagctttt aatgcttcaa
      301 taaaatctcc ctgcgtacct atagcccaag ccactcctcc tccaggaaga gaattaatgg
      361 ctacacatcc agcatatcgt ttccaatcaa ctttctccat tctaggaaca ataatatgtt
      421 cataaaagac tcttgctgct cttccttgtt cgttatcttg actgattcgt tcacgaatag
      481 caccaaaatc ctttatatgg tatacaccat gttcgtctaa aaaaccaact gtatcaaaat
      541 aaaaagttaa ttcatctact aatttattct ttatttctaa atcaactgta ttcacttttt
      601 ctgtggcata cacacttgtt ccagtactaa caaaagtact taaacctaat aagaaaacac
      661 ctaaaacacc aaatatttta attattcttt tcataatttc tcctccataa aaaataagtt
      721 actttttaaa tatcttg
//
LOCUS       DAZKNM010000366          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000366 DAZKNM010000000
VERSION     DAZKNM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.381"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..127
                     /locus_tag="ACG8M9_002939"
     tRNA            38..127
                     /locus_tag="ACG8M9_002939"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            142..213
                     /locus_tag="ACG8M9_002940"
     tRNA            142..213
                     /locus_tag="ACG8M9_002940"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Glu,seq:ttc)
     gene            229..301
                     /locus_tag="ACG8M9_002941"
     tRNA            229..301
                     /locus_tag="ACG8M9_002941"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Val,seq:tac)
     gene            311..383
                     /locus_tag="ACG8M9_002942"
     tRNA            311..383
                     /locus_tag="ACG8M9_002942"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Asp,seq:gtc)
     gene            419..494
                     /locus_tag="ACG8M9_002943"
     tRNA            419..494
                     /locus_tag="ACG8M9_002943"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Phe,seq:gaa)
     gene            496..576
                     /locus_tag="ACG8M9_002944"
     tRNA            496..576
                     /locus_tag="ACG8M9_002944"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Tyr,seq:gta)
     gene            583..656
                     /locus_tag="ACG8M9_002945"
     tRNA            583..656
                     /locus_tag="ACG8M9_002945"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:615..617,aa:Trp,seq:cca)
     gene            659..731
                     /locus_tag="ACG8M9_002946"
     tRNA            659..731
                     /locus_tag="ACG8M9_002946"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:His,seq:gtg)
BASE COUNT          161 a          172 c          201 g          202 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg ccaaactgtc tcaagggttc gaatccctta
      121 ctctccgtag caaatttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
      181 gcggtaacac gggttcgaat cccgtacggg tcattttaat tgaatgttgg aggtttagct
      241 cagctgggag agcatctgcc ttacaagcag agggtcagcg gttcgatccc gttaacctcc
      301 ataatgaact ggttccgtgg tgtaggggtt aacatgcctg cctgtcacgc aggagatcgc
      361 gggttcgatt cccgtcggaa ccgtcgttct tgttattcaa ttaataattt taaaatatgg
      421 cgcggtagct cagttggtag agcaacggat tgaagctccg tgtgtcggca gttcgattct
      481 gtcccgcgcc accatggagg agtagcgaag tggctaaacg cgacggactg taaatccgtt
      541 ccttagggtt cagtggttcg aatccactct cctccatttt ataggggcat agtttaaagg
      601 tagaacagcg gtctccaaaa ccgttagtgt gggttcaatt cctgctgccc ctgccatggc
      661 gaatgtggcg aagtggttaa cgcaccggat tgtggctccg gcattcgtgg gttcgattcc
      721 catcattcgc cctttt
//
LOCUS       DAZKNM010000367          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000367 DAZKNM010000000
VERSION     DAZKNM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.382"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8M9_002947"
     CDS             <1..>725
                     /locus_tag="ACG8M9_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0379617.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKNM010000368          719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000368 DAZKNM010000000
VERSION     DAZKNM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.383"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..289
                     /locus_tag="ACG8M9_002948"
     CDS             53..289
                     /locus_tag="ACG8M9_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0379618.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            286..684
                     /locus_tag="ACG8M9_002949"
     CDS             286..684
                     /locus_tag="ACG8M9_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0379619.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          259 a          114 c          130 g          216 t
ORIGIN      
        1 tggatataat gtacttacta tagaatgaga atggtataga gggtggcgaa atatgaaaaa
       61 aatgaaaaag tataaaatta gaaaatctgg caatagcgat gtaacaacta tcccaccaga
      121 agtaaaagaa ttcatgggtg tgcagacagg tgacgcaatc agttatgttt ttcaatctga
      181 tggttctgta cgaatgatca aagcacaaga agaacctgat attgattcat tagttgattc
      241 gatcatgaat caatatgaag acgcgttaaa agatttggtt gatttatgag gtatctttcg
      301 actaaagaaa ttatcaaaat caacgcgaaa gttattttac gtcactcgcc aggcgaaatg
      361 atcggtgtaa aagatgcaaa tgcattagat atggccgtca aacagccttc acaagcagta
      421 ttcaatcaag aactctatcc tgaaatctat gaaaaagcgg cgattttagc aattaattta
      481 gctaaaaaac atccttttca taatggcaat aaacgaactg ctcttgttgc catgttgttg
      541 tttcttcaat tgaataattg tccagttgct ttttctcgtc aagaagctgt tgattttatc
      601 ataatgatta ccactagttc attggatttc gattcactaa agtccaagat aaccagttat
      661 ttacgacaaa ccgcaataaa atgaacgaat taaaaacttt atttctcaat tagatcagc
//
LOCUS       DAZKNM010000369          717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000369 DAZKNM010000000
VERSION     DAZKNM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.384"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8M9_002950"
     CDS             <1..608
                     /locus_tag="ACG8M9_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0379620.1"
                     /translation="IGTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGT
                     GVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRL
                     GKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFA
                     KLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          201 a          142 c          182 g          192 t
ORIGIN      
        1 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
       61 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      121 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      181 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      241 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      301 aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      361 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      421 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      481 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      541 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      601 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      661 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DAZKNM010000370          694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000370 DAZKNM010000000
VERSION     DAZKNM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.385"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..236)
                     /locus_tag="ACG8M9_002951"
     CDS             complement(<1..236)
                     /locus_tag="ACG8M9_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0379621.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKF"
     gene            complement(254..574)
                     /locus_tag="ACG8M9_002952"
     CDS             complement(254..574)
                     /locus_tag="ACG8M9_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379622.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          238 a          126 c           93 g          237 t
ORIGIN      
        1 tcaaattttg agtgaaaagt tgtagaagta tcgtacaaaa ctgaaaattc aaaatgagag
       61 gcgctgaaat gtgcaagttc atgagataaa tgaaaggctt ctgcagtttc accgtataga
      121 ttttcattca aaaaaatgat tctgggtttt ggatagtaga aaccaggctc tttcatctcc
      181 atataaacta ctttcaaatt gtattcgctc aacatttctt tcaatttcaa atacatacaa
      241 accatcactc caactattca ttttcctcta aagctttagc aattgcaatc gctttacgca
      301 ttgtctcctt agatatttct tttccatcaa aagaaaaaac agtatcgtct tctgataaat
      361 ccacatgttt aggggtttct ctttcttctc tacctagaag gtagtctaca gagacatcga
      421 aatagtcagc aatccgactt aattcatctg aattaggagt attatttttc cactttgcta
      481 gataaccgtt agaatatcca aaattaattt ctaactgccg tatagatatc tttttcctct
      541 tcgccaagtc ttttattatt tcataggtat tcatcgataa aacaaccttt ctaaatgttt
      601 acaaaaaaag tttagaaaaa tacgcagaaa taatttgact aattctgagt aatgagctat
      661 actatatctt gtaaacagat ttaacaacta aaaa
//
LOCUS       DAZKNM010000371          690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000371 DAZKNM010000000
VERSION     DAZKNM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.387"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8M9_002953"
     CDS             complement(<1..461)
                     /locus_tag="ACG8M9_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0379623.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          219 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac
//
LOCUS       DAZKNM010000372          667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000372 DAZKNM010000000
VERSION     DAZKNM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.388"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>667
                     /locus_tag="ACG8M9_002954"
     CDS             <1..>667
                     /locus_tag="ACG8M9_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0379624.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIAD
                     RLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLP
                     QIQ"
BASE COUNT          204 a          127 c          138 g          198 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaacgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aattgattgt tattgatatg tatgcgcctt atgtttcgtt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgat cgtttccaca tcgtccaaca tattggcaga actttcttga
      301 atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa ttcccactct caacgagggt
      361 tagggaaaaa attgaagcgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 atgaaaaacg ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgccg
      481 atcgactgtt agctgcttgt cccaacttac gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcac tatttgagga ttgtttaact agagacatta
      601 gcggtctgcc agaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaa
//
LOCUS       DAZKNM010000373          666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000373 DAZKNM010000000
VERSION     DAZKNM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.389"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>666)
                     /locus_tag="ACG8M9_002955"
     CDS             complement(<1..>666)
                     /locus_tag="ACG8M9_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379625.1"
                     /translation="ISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEI
                     YIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKAT
                     VLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLF
                     LGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFS
                     SLF"
BASE COUNT          280 a          108 c          107 g          171 t
ORIGIN      
        1 taaataaaga agaaaaacca tttaaccgcc agacaaattg tagactctta actggtgtat
       61 ggttaaaaag ttgccaagga aataaattgg ttgaacaaaa aagaacaaat acaccaagaa
      121 agaaaataaa taaatcatca gcagttaatt tttttataaa aataaaagca ccaatcaatc
      181 cgagaaataa gaataatcca atagtatagt tgttatagtt attatttaaa atatgtgtca
      241 gcatatcact aggagccata ccacttaatt cttttcccag tgggactttc aattcttgtg
      301 ctctagattg ttgaataaaa ggaattaaga atcccgcact taaaaaaaag gtgaggacgg
      361 tagcttttaa aagacttaag agtctagcga ttttttggtc ccaaaaataa aaagaaagaa
      421 acaacgttcc tccaataaat acagaaacca tagcgacaga aagtaaatga gtatacacta
      481 ctagcgtcat gccaatagac aaccagtacc atttttggta atctctaata taaatttcgt
      541 aacaccccat tagtatcaaa ggcaaaaatg ttaaagcgac agcttctccc aatgaagcac
      601 gatggaatat gtcaatggca cgataggcgg aaaatgtata aataatcgaa aataacagcg
      661 aaatat
//
LOCUS       DAZKNM010000374          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000374 DAZKNM010000000
VERSION     DAZKNM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.392"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8M9_002956"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8M9_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0379626.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKNM010000375          650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000375 DAZKNM010000000
VERSION     DAZKNM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.393"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          221 a           81 c          146 g          202 t
ORIGIN      
        1 atagtttacg tcgatactta ttaagttatc gacgtttttt attatagaaa aaatttataa
       61 aaggaggttt cttgatatga taagaaacaa aaagggtttg ttgaaagttg ctttactggc
      121 agcttttttc taagtaactt tttgacaagc tcaatggcaa cacaagcgga agaagtaata
      181 caagaactaa ctgttatgaa taacagctag ataaaatggg ctatgtatgt tatccaacaa
      241 tttagtaatc actagaagcc tagcgaaagt tcgcttgata agttatagat gagtattgat
      301 ttgtagtgtt tccaaatcaa aagggtacag agccacatga aatatgttgt cctctgcagc
      361 gtcaggatgg gaatccatac catggagatt cggagatctg tgagggaata cttacgtggt
      421 taatatgtca gttgtgttgg acgtagtaat ggtcatagtg ggtagattga ctgaattctt
      481 atatgagaaa atgaaaccaa caagaagata gggttattta tacagaattg gggatctttt
      541 ctggaaaagt aatcggataa tgcgagattc gtacggtctt ttttaaattt ctctaaaaaa
      601 aacggcatcg aatgggttgg gtgattgata agcaatgaat catagactga
//
LOCUS       DAZKNM010000376          633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000376 DAZKNM010000000
VERSION     DAZKNM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.395"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..363
                     /locus_tag="ACG8M9_002957"
     CDS             <1..363
                     /locus_tag="ACG8M9_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0379627.1"
                     /translation="KFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTI
                     PISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHA
                     SLFMNEFQDIIHRVDDWI"
BASE COUNT          218 a           81 c           97 g          237 t
ORIGIN      
        1 aaatttacta acatttggac tgaatctgat aaaaacttca tttcttttta taataattat
       61 aaaaatgact tgcttgaata taaagataaa gaagaaatgt ttcctaaaaa accgatacct
      121 gaaaacacca taccgatttc aatgattcct tggattgatt ttagttcatt taatttaaat
      181 attggtaaca atagcagctt tttattgcct attattacga taggtaaatt ttatagtgag
      241 aataataaaa tttatatacc agttgctctg caacttcatc attctgtatg tgatggttac
      301 catgcttcac tatttatgaa tgaatttcaa gatataattc atagggtaga tgattggatt
      361 tagtttttag attttgaaag tgaatttaat tttatacacg taagtgatca taaaatttat
      421 gaacgtataa caaccacatt ttttggttgc ttgtggtttt gattttgaat ttggttttga
      481 acttatggac tgatttattc agtccatttt ttgtgcttgc acaaaaacta gcctcgcaga
      541 gcacacgcat taatgactta tgaaacgtag taaataagtc tagtgtgtta tactttactt
      601 ggaagatgca ccgaataaaa aatattgaag aac
//
LOCUS       DAZKNM010000377          598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000377 DAZKNM010000000
VERSION     DAZKNM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.396"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..586
                     /locus_tag="ACG8M9_002958"
     CDS             <1..586
                     /locus_tag="ACG8M9_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0379628.1"
                     /translation="STGIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFT
                     DDNPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWH
                     IANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKK
                     VYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          206 a          108 c          122 g          162 t
ORIGIN      
        1 atccacagga atcaaatcgc atttatgggt aacacaaaat atttggggcg atcatacaga
       61 tccttgcggt tatctttctg aaatgggtgt aagtaaagaa aaactagcct atgatttggc
      121 tcatggtttt acggatgata atccaactac ttcggatgat aatccaacta cttcggagga
      181 taaaccagta attgatccaa ctcgagcagg tgctgcaaat tctacgctga cagatggaac
      241 aaattacgcc cacgttgatc agttcggaga aatcgaaaac gcaaacttgc atgtagctgg
      301 atggcacatt gctaattata aatacgagta tattttcatt atggactaca atactggaaa
      361 agaactagct agagtaagag ctgatgggat ttatagacca gacgtaaatc aagcttataa
      421 tactttagga aatgttggct atcatgtatc tttcaatatg cgtgatttcc ctaataagaa
      481 agtatacgtc atgatgcgtg cgacaaacga tccagaagga aatactaagg gtggagcaca
      541 agattttcat gacaagcgtt ggtatttaaa tattccacaa cgataaaaaa ttcccccc
//
LOCUS       DAZKNM010000378          592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000378 DAZKNM010000000
VERSION     DAZKNM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.397"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..358)
                     /locus_tag="ACG8M9_002959"
     CDS             complement(<1..358)
                     /locus_tag="ACG8M9_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0379629.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQH"
BASE COUNT          175 a          105 c           85 g          227 t
ORIGIN      
        1 gatgctgttt agcaaaattt tctactgaga tatatttttc ctgaaagaaa gccaatacca
       61 ttttcataga taaagaatcc gttatataac gagataacag agctttgcta gaacattttc
      121 cattaatttc cagacatata tccaattcac tgacggttac tcgcaaattt ttctctagat
      181 cgaacttttg taaatcatgt ttcagcttat cgatcgtttt tttcaagaga aatgtcgata
      241 gcgacaactc ttcttgaaca ttcgatattg actctgataa atttgagctt gcttctaaat
      301 atttaagtaa tgaataactt ctgtgatctt ctttgcttaa aaaaaactca taattcatta
      361 gtcatcagct ctttctacgt atattacttt ccatgcattc accaactagc tggggataaa
      421 actgatttcc tggatccgag tcactgttgt cagcgattct tgtttgtata atatacatcg
      481 tcctgtcttt tttttaacat tttggagagc gttttatttg gcctttctcg cgatcaaacc
      541 tgttattaga attatatcat ttactagtat gcatttttat taataaaatg gt
//
LOCUS       DAZKNM010000379          591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000379 DAZKNM010000000
VERSION     DAZKNM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.398"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..508
                     /locus_tag="ACG8M9_002960"
     CDS             <1..508
                     /locus_tag="ACG8M9_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFQ0379630.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          181 a          108 c          130 g          172 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
      541 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga a
//
LOCUS       DAZKNM010000380          583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000380 DAZKNM010000000
VERSION     DAZKNM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.399"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>583)
                     /locus_tag="ACG8M9_002961"
     CDS             complement(38..>583)
                     /locus_tag="ACG8M9_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0379631.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DAZKNM010000381          583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000381 DAZKNM010000000
VERSION     DAZKNM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.400"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..430
                     /locus_tag="ACG8M9_002962"
     CDS             29..430
                     /locus_tag="ACG8M9_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0379632.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            424..>583
                     /locus_tag="ACG8M9_002963"
     CDS             424..>583
                     /locus_tag="ACG8M9_002963"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379633.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVG"
BASE COUNT          217 a           88 c          111 g          167 t
ORIGIN      
        1 ggaaaggggg aaaaaggagc atattatcat gcaacaacat tacaatgaca tatctataca
       61 acaccaattt gatgcctact gtaaaaaagt attacgaaac gaagctaagt ctattcgaaa
      121 acgaaacgct aacataaagg ataatgagga accattggca gattcaaatg aaggcaggta
      181 ttcccaaact cattttgatg aacaagatgt ctatttctta tttggaatgg aaatattaat
      241 ttcagatcaa aagttatcag cagcaattga tcagttatcg gagataagaa gaaagattat
      301 cctactttat tattttgcag gttttaatga tgctgaaatc ggtaaaattc tcaatatgag
      361 cacaagtggt atctggtatc aacgaaagaa ggcagttgaa caattgaaaa tggggtatga
      421 cctatggtag ataaatctaa aaagcggcag tacctattag ttccactatc cattatctta
      481 tctgctctag aaggagatcc aatttcgatc aagatgatac tcaaacatta tcattcttat
      541 atggctaagt tatgtttgac aaatggattt aacgaggtag gtc
//
LOCUS       DAZKNM010000382          579 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000382 DAZKNM010000000
VERSION     DAZKNM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.401"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..454)
                     /locus_tag="ACG8M9_002964"
     CDS             complement(155..454)
                     /locus_tag="ACG8M9_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0379634.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          185 a          107 c           70 g          217 t
ORIGIN      
        1 tttttgtgtt aattaaagga taaaatacaa tataaagaaa catctgtttc tttatttaga
       61 ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta
      121 tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg
      181 ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat
      241 aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt
      301 ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta
      361 tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta
      421 gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt
      481 cttcccacta cattctataa acaaagtata cgctacatct agattttttt aaatcattac
      541 tggattgtta aaacctttca atacactgtt atggcaacg
//
LOCUS       DAZKNM010000383          570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000383 DAZKNM010000000
VERSION     DAZKNM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.402"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(354..>570)
                     /locus_tag="ACG8M9_002965"
     CDS             complement(354..>570)
                     /locus_tag="ACG8M9_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379635.1"
                     /translation="DKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRFIGV
                     DTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          213 a           87 c          104 g          166 t
ORIGIN      
        1 agaggaataa ataatagtgg agaaaactat ggtttggcgc agattctatc aggtttgatg
       61 gaacgaagac aacctcccga catcaagtca aaagctactt gagattttga tctgaaaata
      121 aaaaaagaaa acaaggtagg aacatcaatg tttctacctt gttttcgtaa tcaatgggaa
      181 aaaactaaga gtggtgtacg taatcaataa aaaggatggt gacaacagta cctctttact
      241 ctctgaaagc atcataactc gtttttcctc attatgccaa taaattgttg ttataccaca
      301 tataaggatt tgtaatagct aaaaacagac aggaaagttg aaatctgtga atcttatttt
      361 gtatggaagg atttatagaa cgcaaaaatc aagataaaga tgaataaaaa tcctgtttcg
      421 gctatatatt gcgtatctac gccaataaaa cgagtttgtt ctgtcatagc catataaagc
      481 aaaggcaaaa atacaactac agcgagataa gatccgatga tttctcgaaa agttttccag
      541 gaagtatgtt tcctgttttt atatttatcc
//
LOCUS       DAZKNM010000384          554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000384 DAZKNM010000000
VERSION     DAZKNM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.403"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..239
                     /locus_tag="ACG8M9_002966"
     CDS             <1..239
                     /locus_tag="ACG8M9_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0379636.1"
                     /translation="FYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPAAKTRMKKDVY
                     FLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(275..>554)
                     /locus_tag="ACG8M9_002967"
     CDS             complement(275..>554)
                     /locus_tag="ACG8M9_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379637.1"
                     /translation="SRAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFSTVKE
                     LNTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          173 a           94 c           81 g          206 t
ORIGIN      
        1 atttttataa ggtgtgggat tcagaagttt tctttttagc aatcgaagat agcttattaa
       61 ttaatctcta ctattctaat aagaactact ttaaaatccc tgctgcgaaa actagaatga
      121 agaaagacgt atattttttg tttgatatcg tgactgatgt gccggacgca cgtagcgatc
      181 atcggcgtta tgactatata aagtatactt tcgttgatcc agaaagatac aaagattaaa
      241 taaaaaaata acaaacaaac tcgcttgaaa ttcattaggt gagtttgttt attatttttt
      301 ctgcttctcg attcatcagc gccatatctg ttttcgtaat ggtttcatta ggaatatagt
      361 cagcacattt tcttttctct aacattctat acccactatt atgtattcct aaggtattca
      421 attccttaac tgttgaaaac aaattccaaa cggtttggtg gcttcctttt ccgctacttc
      481 cattataaat aatatcataa ttttctttca aatactcatc agatttttta aaagcactgt
      541 agtatgctct tgaa
//
LOCUS       DAZKNM010000385          551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000385 DAZKNM010000000
VERSION     DAZKNM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.404"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..524
                     /gene="spxA"
                     /locus_tag="ACG8M9_002968"
     CDS             126..524
                     /gene="spxA"
                     /locus_tag="ACG8M9_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0379638.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           87 c          110 g          158 t
ORIGIN      
        1 taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga
       61 tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt
      121 gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt
      181 ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca
      241 gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga
      301 cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat
      361 tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata
      421 aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac
      481 gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac
      541 ttggaaaaga c
//
LOCUS       DAZKNM010000386          546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000386 DAZKNM010000000
VERSION     DAZKNM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.405"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..120
                     /locus_tag="ACG8M9_002969"
     CDS             <1..120
                     /locus_tag="ACG8M9_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0379639.1"
                     /translation="ANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            121..>546
                     /locus_tag="ACG8M9_002970"
     CDS             121..>546
                     /locus_tag="ACG8M9_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0379640.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSV"
BASE COUNT          208 a           64 c          104 g          170 t
ORIGIN      
        1 gctaatgata tgtatgagaa ggagttgaaa tatttctttg aaaacgtagt aacaggcaaa
       61 gaaaactgga atgatttata tcatgcaaac gaagtattga aaatagctga ggagaaataa
      121 atggaaattt tatttactat ttgtggtaga gctgggtcta aggggtttaa aaataaaaat
      181 ttgaaatatt tggcaggaaa gcccctagtc catttcgctt tagcaatgat agatatctat
      241 caacaaaaac atcctgaaca taattgcacg acttgtttga atacggacag taatgagtta
      301 gtagaattga cgactaagtt aaatccgtct gtgaaaattg tagaacgtaa acctgaatta
      361 gctacagata ctgtagggaa aattgaagtg atacgtgatt cattcttaca actagaaaga
      421 gaagataata tatttgattt aattatagat ttagatttga cttcaccttt acgtgaagtt
      481 tctaatcttg aagaattaat agatgaacat ctaaaaaatt tagataaaga cgtctacttt
      541 tcagtt
//
LOCUS       DAZKNM010000387          537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000387 DAZKNM010000000
VERSION     DAZKNM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.406"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACG8M9_002971"
     CDS             <1..365
                     /locus_tag="ACG8M9_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0379641.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
BASE COUNT          211 a           78 c          117 g          131 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacaga
//
LOCUS       DAZKNM010000388          536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000388 DAZKNM010000000
VERSION     DAZKNM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.407"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>536
                     /locus_tag="ACG8M9_002972"
     CDS             <1..>536
                     /locus_tag="ACG8M9_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0379642.1"
                     /translation="NEYYDEIIEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKIS
                     YQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQK
                     LSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLF
                     SWNEETSSMTDIQLYDEV"
BASE COUNT          199 a           63 c           95 g          179 t
ORIGIN      
        1 aaatgaatat tatgatgaaa taatcgaagt agctaaaaag aaagatagat tactttatct
       61 atcttctaca tttttgaaga gaaaagaaat tcaatatatt aagtctaaag tgacgcataa
      121 taaaaaaatt tcttatcaat accatgtagg acagtattta cccgactggc atccttggga
      181 agattatcgc aatttttttg tttcaaacaa aaaaactaat gggtgtagag aaatattcgc
      241 tattgaatta ccttggatta tagatacatt tgggaaagtt aaatcgttta ctgtaatgac
      301 ccagaaatta tccaatcttg atattgatta ttcggacagt ttcactctta ttattgagca
      361 tgaaactggt atagttggaa ctttaagtgt agatgttgtt tcaagagtag ctaaaagaaa
      421 tttaactata attggtgaaa agattttaat agaatgggaa ggtaacccgg attctctatt
      481 ttcatggaat gaagaaactt ctagtatgac agatattcaa ttgtacgatg aggtta
//
LOCUS       DAZKNM010000389          532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000389 DAZKNM010000000
VERSION     DAZKNM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.408"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACG8M9_002973"
     CDS             112..306
                     /locus_tag="ACG8M9_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379643.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          227 a           68 c           86 g          151 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa at
//
LOCUS       DAZKNM010000390          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000390 DAZKNM010000000
VERSION     DAZKNM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.409"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>523
                     /locus_tag="ACG8M9_002974"
     CDS             <1..>523
                     /locus_tag="ACG8M9_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0379644.1"
                     /translation="VDYIKEKVTLGWTPDVIIGRKERSVSCGMRTLYRLFSKGIFDID
                     TLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVI
                     TLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISN
                     QHDIDIYFADPGTP"
BASE COUNT          173 a           98 c           98 g          154 t
ORIGIN      
        1 aagtagatta cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc
       61 gaaaagaaag gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa
      121 tatttgatat tgacacacta ccgatgaaag gtaaaagaaa acccaatggc catcaggaaa
      181 aacggggaaa acaacagtat cagcgctcaa tccatgatcg acctgataat tatcctgatt
      241 tcaattctga gtttggtcac cttgaaggtg atacgatcgt tggcattcat cataaaagtg
      301 ccgtcattac tttagttgaa agattatcta aagtcattat cacgattaaa cccaacggcc
      361 gtaaggcatt agatattgaa actgccctta atcaatggtt ttctcgcttc cctaaaaact
      421 tctttaaatc tattacgttt gactgtggaa aagaattttc taactggaaa gccattagta
      481 accaacatga tattgatata tattttgcgg accctggaac acc
//
LOCUS       DAZKNM010000391          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000391 DAZKNM010000000
VERSION     DAZKNM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.410"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8M9_002975"
     CDS             complement(<1..132)
                     /locus_tag="ACG8M9_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0379645.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKNM010000392          505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000392 DAZKNM010000000
VERSION     DAZKNM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.411"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>505
                     /locus_tag="ACG8M9_002976"
     CDS             <1..>505
                     /locus_tag="ACG8M9_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379646.1"
                     /translation="LHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIY
                     HPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASING
                     KLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQ
                     KQPRREVQ"
BASE COUNT          193 a           87 c           64 g          161 t
ORIGIN      
        1 attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat taacacttat
       61 agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt atgcaaacaa
      121 agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag gtaaccagga
      181 aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag ataattcgaa
      241 taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag tggcttctat
      301 taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat taactattcc
      361 tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa ttgcttcaag
      421 atatttatct atcgttactt tgattttatt tttactttat cgatttactt tttctaaaca
      481 aaaacaaccg agaagagaag tacaa
//
LOCUS       DAZKNM010000393          493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000393 DAZKNM010000000
VERSION     DAZKNM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.413"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..173
                     /locus_tag="ACG8M9_002977"
     CDS             51..173
                     /locus_tag="ACG8M9_002977"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379647.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
BASE COUNT          182 a           73 c           90 g          148 t
ORIGIN      
        1 acgggttaaa tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac
       61 cttttccgga ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat
      121 ttatattggt gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg
      181 tttagctaaa tggggagcca aaaatataga aaattttcaa agtgtagata taattattga
      241 ttctcctcaa gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata
      301 gggtctcacg ctctttgttt ttcgccattt aaagattgaa atttttgcca tcgccgattg
      361 taaaattaag ctagacaact aaaaaagtca tttgtgctac actcaaaata gttccgacca
      421 aagaattata aggagtgagt acaaatgacc tatacccatc ttacaacgga tgaacttgta
      481 ataatagagt ctt
//
LOCUS       DAZKNM010000394          474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000394 DAZKNM010000000
VERSION     DAZKNM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.414"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8M9_002978"
     CDS             132..344
                     /locus_tag="ACG8M9_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379648.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT          149 a           81 c           88 g          156 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tata
//
LOCUS       DAZKNM010000395          474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000395 DAZKNM010000000
VERSION     DAZKNM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.415"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>474
                     /locus_tag="ACG8M9_002979"
     CDS             <1..>474
                     /locus_tag="ACG8M9_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0379649.1"
                     /translation="IACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSN
                     LAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKS
                     YFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          156 a           86 c           84 g          148 t
ORIGIN      
        1 ttattgcttg tgttatatgg ggcataatta atggctatac tagccaaaga gccacgtata
       61 gagcggtttg ttccgtctta tttcctaatg gtgactttcc tagtaggagt cgattcactc
      121 gcttaagttc aaacttagct tacactatca agattattcg atactttttc atcaagaagc
      181 tcaccaaagg tgaactagtc ggaattatag atagttttcc tagtccatta tgtaaaccag
      241 ttagaaatag acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa
      301 aaaaatctta tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga
      361 ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag actttatcgg
      421 aagaagcgaa tcttcccaat atcctagggg acaaaggata tattagccat aaaa
//
LOCUS       DAZKNM010000396          469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000396 DAZKNM010000000
VERSION     DAZKNM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.416"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..>469
                     /locus_tag="ACG8M9_002980"
     CDS             170..>469
                     /locus_tag="ACG8M9_002980"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0379650.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPT"
BASE COUNT          178 a           56 c           79 g          156 t
ORIGIN      
        1 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
       61 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      121 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      181 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      241 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      301 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      361 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      421 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacc
//
LOCUS       DAZKNM010000397          457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000397 DAZKNM010000000
VERSION     DAZKNM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.418"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          146 a           80 c           58 g          173 t
ORIGIN      
        1 ataaactatt ttttatataa gttttacatt catcatgatt catacaaact ccaccttcta
       61 taaatgaata caaaaaaagc aatcaaacga tttccgattg attgcttaac aattcttaaa
      121 ttcagtagct tagatacttg aaaactctct gatttcccta tataatgata gtacggttat
      181 ataccgtctt caaacaaagt taattaaata acttcttacg agggaagagt tcatctgact
      241 aactgataag cgttggtttg gcaatcttat cgggctatgc atttataaaa tgtcgtcaaa
      301 cattttataa atgtgtcatg gctctttttt cgtttctatt cagttcgttg tttcgttata
      361 tctagtatac cgcttttaaa aaaaataagc aacgatttcg tgcattattc acacgaagtc
      421 attgcttttt tcttcttcca tttctaaatc caatgtt
//
LOCUS       DAZKNM010000398          451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000398 DAZKNM010000000
VERSION     DAZKNM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.420"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..142)
                     /locus_tag="ACG8M9_002981"
     tRNA            complement(71..142)
                     /locus_tag="ACG8M9_002981"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Gln,seq:ttg)
     gene            complement(152..224)
                     /locus_tag="ACG8M9_002982"
     tRNA            complement(152..224)
                     /locus_tag="ACG8M9_002982"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(189..191),aa:His,seq:gtg)
     gene            complement(286..370)
                     /locus_tag="ACG8M9_002983"
     tRNA            complement(286..370)
                     /locus_tag="ACG8M9_002983"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Leu,seq:caa)
BASE COUNT          125 a          118 c          107 g          101 t
ORIGIN      
        1 caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc ggcaaattta
       61 aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc
      121 cttaccgctt ggctataccc caagaataaa aggcgaatga tgggaatcga acccacgaat
      181 gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat aaaagatgtt
      241 atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg gcggccgggg
      301 tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct gccaattccg
      361 ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa ccggtgatga
      421 aggttttgca gacctctgcc ttaccacttg g
//
LOCUS       DAZKNM010000399          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000399 DAZKNM010000000
VERSION     DAZKNM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.422"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..437
                     /locus_tag="ACG8M9_002984"
                     /pseudo
     CDS             1..437
                     /locus_tag="ACG8M9_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DAZKNM010000400          434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000400 DAZKNM010000000
VERSION     DAZKNM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.423"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>434
                     /locus_tag="ACG8M9_002985"
     CDS             82..>434
                     /locus_tag="ACG8M9_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0379651.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSI"
BASE COUNT          153 a           49 c           98 g          134 t
ORIGIN      
        1 tgtataattt tgatgtggat tttatcggtt tatttatgtt aaagtataga tgaagatatg
       61 tttttgattg gagggtcacc attgaaaaaa attgcgaagt atatggaaat ttacttagat
      121 ataaagaaaa aaattgtaaa tggagagtat aaaattggtg agaaacttcc tacaggagat
      181 gaattggcag ttatttatca tacaagcaag ttaacagtaa agaaaggcgt agacttactt
      241 gtttccgaag gagttttgcg aagtagaagt gggtatggga ctgaagtcgt cagaacacca
      301 attgataact cacgagttct tggtccaaac gaaggtttgt ttagcgtggt tggtgaagaa
      361 cacgtagatt cagaaatcca tactttctca attgaacttc catcgaaaaa agtagctgat
      421 aaattaagta tcag
//
LOCUS       DAZKNM010000401          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000401 DAZKNM010000000
VERSION     DAZKNM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.425"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG8M9_002986"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG8M9_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKNM010000402          419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000402 DAZKNM010000000
VERSION     DAZKNM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.426"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..350)
                     /locus_tag="ACG8M9_002987"
     CDS             complement(48..350)
                     /locus_tag="ACG8M9_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0379652.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          138 a           77 c           60 g          144 t
ORIGIN      
        1 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
       61 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      121 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      181 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      301 ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat aatgttcacc
      361 tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat tgataatat
//
LOCUS       DAZKNM010000403          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000403 DAZKNM010000000
VERSION     DAZKNM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.427"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8M9_002988"
     rRNA            <1..>411
                     /locus_tag="ACG8M9_002988"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKNM010000404          404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000404 DAZKNM010000000
VERSION     DAZKNM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.428"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>404
                     /locus_tag="ACG8M9_002989"
     CDS             <1..>404
                     /locus_tag="ACG8M9_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0379653.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAZKNM010000405          402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000405 DAZKNM010000000
VERSION     DAZKNM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.430"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>402
                     /locus_tag="ACG8M9_002990"
     CDS             <1..>402
                     /locus_tag="ACG8M9_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0379654.1"
                     /translation="VLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYL
                     ILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVY
                     SFARKIGQALAGGVGGWTLSIIGYDSLANVQT"
BASE COUNT          120 a           45 c           83 g          154 t
ORIGIN      
        1 agttttaatg aattttatca atcctatttt ggttttaata gttgtttcgc ctttaacttt
       61 gtttttaagt aaaaaatatg gaaaaaaaga actggcatca gtaggaatgt tcttttcagc
      121 aatagtatat ttgatcttat ttttgataaa agctagtaat atgtatgtat tcttaattct
      181 tagttcggtt ggctatatgg gtttgggtgt gtttaatact gttatttggg caaatattac
      241 tgatgttatt gatgatcaag aagtaaagtc tgatcaaaga gaagatggga ccgtatatgc
      301 ggtatattct tttgctagaa aaataggtca agctctagct ggaggcgtcg gaggttggac
      361 tctttctatt ataggctatg actctttagc aaatgtacaa ac
//
LOCUS       DAZKNM010000406          399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000406 DAZKNM010000000
VERSION     DAZKNM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.431"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..368
                     /locus_tag="ACG8M9_002991"
     CDS             <1..368
                     /locus_tag="ACG8M9_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379655.1"
                     /translation="VAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRKKETF
                     LIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRKGRHT
                     TTFSSTDSLEQSRRTRRRR"
BASE COUNT          120 a           58 c           90 g          131 t
ORIGIN      
        1 tagtcgctgt tgcacagtcg ctgcttgata tactcaagtg gctgatggaa ggaagcaatt
       61 ggatatttgt gttgccacta ttgcttatta gtctatttat catgtttcgc atggtcagaa
      121 aaaaagaaac gtttctgatt tttatctggt atgcagcatc cgttggggtt gctaccttga
      181 tggttgaatt ggcgttaggt agttgggaag cttgcgtatt tgctttattt tttgctgtac
      241 tgtatctaat catttatatc ccatatatga aaagaaagtc tcggataggc agaaaaggta
      301 gacatacaac tactttttct agtacagatt ctctcgaaca aagtagaaga acgagaagaa
      361 gacggtaata tttagtaggt atacgcaaaa aaagataaa
//
LOCUS       DAZKNM010000407          393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000407 DAZKNM010000000
VERSION     DAZKNM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.432"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>393)
                     /locus_tag="ACG8M9_002992"
     CDS             complement(<1..>393)
                     /locus_tag="ACG8M9_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0379656.1"
                     /translation="ENQIICHPIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCI
                     LTKSMLHRVKFVNCKLTGTKLMESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSS
                     ADFVDCSLTKIMFSTNDLENASFFDTDLK"
BASE COUNT          138 a           63 c           57 g          135 t
ORIGIN      
        1 ttttaaatca gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt
       61 agtgagggaa cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc
      121 ttttatgttt gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt
      181 cccaatataa ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg
      241 atgcaacatg ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc
      301 ggtaatctcc attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt
      361 gtaaataatt ggatggcaga ttatttggtt ttc
//
LOCUS       DAZKNM010000408          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000408 DAZKNM010000000
VERSION     DAZKNM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.434"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8M9_002993"
     CDS             <1..>386
                     /locus_tag="ACG8M9_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0379657.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKNM010000409          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000409 DAZKNM010000000
VERSION     DAZKNM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.435"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8M9_002994"
     CDS             <1..>386
                     /locus_tag="ACG8M9_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0379658.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKNM010000410          372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000410 DAZKNM010000000
VERSION     DAZKNM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.436"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..271
                     /locus_tag="ACG8M9_002995"
     CDS             32..271
                     /locus_tag="ACG8M9_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0379659.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          158 a           38 c           81 g           95 t
ORIGIN      
        1 aataagaata agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa
       61 agaaaaacga aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt
      121 tcgtgttcgt gctgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa
      181 cgtaaaagaa gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca
      241 ttatcttaaa attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat
      301 taagaaggag ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac
      361 tgttttgaaa ca
//
LOCUS       DAZKNM010000411          370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000411 DAZKNM010000000
VERSION     DAZKNM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.437"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          143 a           52 c           65 g          110 t
ORIGIN      
        1 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
       61 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      121 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      181 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      241 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      301 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      361 gattagagat
//
LOCUS       DAZKNM010000412          370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000412 DAZKNM010000000
VERSION     DAZKNM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.438"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          131 a           57 c           62 g          120 t
ORIGIN      
        1 tatgtcctaa acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg
       61 catgcgtatg tcttagtcca tcaataaaca gtcgaagaga aaatagggaa aaattgatta
      121 ctaaagaaaa acttactagg tcgacggtgt agttgatttt catacttcaa tagattcgaa
      181 ttaaacgatt tcgattggct tataccagtc ctatccaacg aaagatatat cgttcttaac
      241 tctagcggta taacttgaag ctttacgtaa agccaatggg aatgaactgg agatgacttt
      301 ttattatttt tagtaatagc tgatgggtta atacttacaa ctttcctttt caaagtacca
      361 tataaaaaaa
//
LOCUS       DAZKNM010000413          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000413 DAZKNM010000000
VERSION     DAZKNM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.439"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8M9_002996"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8M9_002996"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKNM010000414          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000414 DAZKNM010000000
VERSION     DAZKNM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.440"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..>362
                     /locus_tag="ACG8M9_002997"
     CDS             76..>362
                     /locus_tag="ACG8M9_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379660.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGR"
BASE COUNT          143 a           60 c           67 g           92 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggta agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
      361 cg
//
LOCUS       DAZKNM010000415          360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000415 DAZKNM010000000
VERSION     DAZKNM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.442"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>360)
                     /locus_tag="ACG8M9_002998"
     CDS             complement(<1..>360)
                     /locus_tag="ACG8M9_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFQ0379661.1"
                     /translation="AISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVP
                     QNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADY
                     VTVSNDKDGVSQAFERIL"
BASE COUNT          123 a           63 c           48 g          126 t
ORIGIN      
        1 aagaattctt tcaaatgctt gagatactcc gtctttatca tttgaaacag tgacataatc
       61 agcttttttc atgatttctt gatgactatt tcccattgca aaactaatat ctgcactttc
      121 aaacatagat aagtcattta aactatctcc gatcgcgatt ctcaaaggtt tctttaaatt
      181 gtagattgat tccaaacgat caaacaccac acctttagat acattttgag gtacgatttc
      241 aatatttaca ggtgccgatt cagtaacaga cacatttagc atctttttca aatggttaaa
      301 tatattagct ttcaattgac gattaggaat aaaaataaaa aatttgtaaa tgctgatagc
//
LOCUS       DAZKNM010000416          360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000416 DAZKNM010000000
VERSION     DAZKNM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.443"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..>360
                     /locus_tag="ACG8M9_002999"
     CDS             118..>360
                     /locus_tag="ACG8M9_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0379662.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          125 a           62 c           72 g          101 t
ORIGIN      
        1 caaaaagaaa aaatagtagt tatttacttg tcatgttact aaataaaaaa ataatttaaa
       61 tatgatatat agtcatttat ttagtgattt cattctaaaa taaagggaga aatgtctatg
      121 ctatacggtg caattgaagc aggcggcaca aaatttgtgt gtgccattgg cgatgaagaa
      181 atgacgatca aggaaagagt gagctttccg acaacgacac ctgaggaaac aatgccttta
      241 gtcattgact tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt
      301 gggcctatcg atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa
//
LOCUS       DAZKNM010000417          353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000417 DAZKNM010000000
VERSION     DAZKNM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.444"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>353
                     /locus_tag="ACG8M9_003000"
     CDS             <1..>353
                     /locus_tag="ACG8M9_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFQ0379663.1"
                     /translation="SYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNN
                     EKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLY
                     SLPVNAEFYEKKTSF"
BASE COUNT          108 a           43 c           69 g          133 t
ORIGIN      
        1 tcttatttag gtcctgctgc ggggggaata tattcctaca ttagtaacat aggtattatt
       61 ctaagcgtaa tatggatgtc tactaataac gcctgggttc catggttcta tggtgagatg
      121 agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact attatttagt agtctttact
      181 ttgattacga tgattgtaat ggtagtttcg attgatttag caaaatttat ggctccgaaa
      241 gactattatt ctgggttatc tttagtggtt ccgataacct taggctattt ctttcagttt
      301 ttatatagtt taccagttaa tgctgaattt tatgagaaaa aaacatcgtt tat
//
LOCUS       DAZKNM010000418          348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000418 DAZKNM010000000
VERSION     DAZKNM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.445"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..176
                     /locus_tag="ACG8M9_003001"
     CDS             3..176
                     /locus_tag="ACG8M9_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0379664.1"
                     /translation="MKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKATTCIAGMS
                     IGGFLGGAIPGKC"
     gene            178..>348
                     /locus_tag="ACG8M9_003002"
     CDS             178..>348
                     /locus_tag="ACG8M9_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0379665.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNY"
BASE COUNT          133 a           39 c           68 g          108 t
ORIGIN      
        1 ctattaaaga gacacaactt atctatgggg gtaccactca tagtggaaaa tattatggaa
       61 atggagtgta ttgcactaaa aataaatgta cggtcgattg ggccaaggca actacttgta
      121 ttgcaggaat gtctataggt ggttttttag gtggagcaat tccagggaag tgctaaaatg
      181 aaaaaaaatg ctaagcaaat tgttcatgaa ttatataatg atatatctat aagtaaagat
      241 cctaaatatt ctgatattct tgaggtttta caaaaggtat atttaaaatt agaaaaacaa
      301 aaatatgaat tagatcccgg tcctttaata aatagattgg tgaattat
//
LOCUS       DAZKNM010000419          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000419 DAZKNM010000000
VERSION     DAZKNM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.446"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG8M9_003003"
     CDS             <1..>344
                     /locus_tag="ACG8M9_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0379666.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKNM010000420          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000420 DAZKNM010000000
VERSION     DAZKNM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.447"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          119 a           57 c           60 g          105 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag g
//
LOCUS       DAZKNM010000421          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000421 DAZKNM010000000
VERSION     DAZKNM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.448"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..>339
                     /locus_tag="ACG8M9_003004"
     CDS             38..>339
                     /locus_tag="ACG8M9_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0379667.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
BASE COUNT          122 a           36 c           62 g          119 t
ORIGIN      
        1 ttatactcta ttaggaaata taagaaggag tataaaaatg aacaaacata atagaaaaat
       61 aacgaataac aaagaactga tcttgttact aatagggatt tggggtatct ttttactctt
      121 taatatgtat atgccgacta tgagagcgga tgacgtagtt tatgcaagta gattagatga
      181 acttggctat ttgggtgctt cgatagaaca ttataagaca tggagttcaa gaattattat
      241 agaactattc ttaatgttct tttctaaaca ctttatgcta tggaaattat taaattctac
      301 gattatgtta ggaattgtag tattactttg caagtatgt
//
LOCUS       DAZKNM010000422          338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000422 DAZKNM010000000
VERSION     DAZKNM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.449"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>338)
                     /locus_tag="ACG8M9_003005"
     CDS             complement(<1..>338)
                     /locus_tag="ACG8M9_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379668.1"
                     /translation="FLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAVLHFFKV
                     ELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQIDDENS
                     FSIFRRQQKIL"
BASE COUNT          104 a           70 c           55 g          109 t
ORIGIN      
        1 agaatctttt gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc
       61 atttgttttt gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt
      121 aaaccttctg gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata
      181 taatctttta ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct
      241 tctgcttgat agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc
      301 agcaatgttt tattttcctg caattgaaca tatagaaa
//
LOCUS       DAZKNM010000423          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000423 DAZKNM010000000
VERSION     DAZKNM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.452"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG8M9_003006"
     CDS             <1..>321
                     /locus_tag="ACG8M9_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0379669.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKNM010000424          316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000424 DAZKNM010000000
VERSION     DAZKNM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.453"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..244)
                     /locus_tag="ACG8M9_003007"
     CDS             complement(83..244)
                     /locus_tag="ACG8M9_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0379670.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          101 a           64 c           33 g          118 t
ORIGIN      
        1 tgataaaaat tattaaagtt ctatttttct ttcttgtaat atatgctata gcatttttta
       61 tcactttcct aactactcct ctctattttc ttactccata actaattgca tccactggac
      121 aagcattttt acatcgtcca catctgatac actccagatg attgcaattc tcaactgaat
      181 ctttattcat ttggcaaact tttttacatt ttccacaatt aatacactta tccttatcct
      241 gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac
      301 tggaggattt ttcatg
//
LOCUS       DAZKNM010000425          304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000425 DAZKNM010000000
VERSION     DAZKNM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.456"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          120 a           45 c           61 g           78 t
ORIGIN      
        1 gggagcttac taaaaaagaa tggagtaaga gttaacacaa tgggccatta gctgaagttt
       61 taagcacttc aaggtatgaa acagaatggc ctgtaatact acatgattat ttagactgct
      121 caaactgcta tcagtctggt ggaatgaata aagtattata ctaaaagcag atccaactac
      181 aacgtaacag gaaaccagtt gtatcaggaa aagtgatagt acatggagaa gaaaaggccc
      241 cctttgtatg agtcagtaca tatcttattt atagataaaa cggtattcaa tcgaaataaa
      301 aaat
//
LOCUS       DAZKNM010000426          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000426 DAZKNM010000000
VERSION     DAZKNM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.457"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..301
                     /locus_tag="ACG8M9_003008"
                     /pseudo
     CDS             1..301
                     /locus_tag="ACG8M9_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DAZKNM010000427          299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000427 DAZKNM010000000
VERSION     DAZKNM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.458"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..260)
                     /locus_tag="ACG8M9_003009"
     CDS             complement(<1..260)
                     /locus_tag="ACG8M9_003009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0379671.1"
                     /translation="MSIVLSFFITIFITLNDNGQSYADNFTIQGLLEYASRDSGYDGV
                     GDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSE"
BASE COUNT          112 a           58 c           32 g           97 t
ORIGIN      
        1 aactcactga caaaaaaatt ggtaaactct gcattgccaa tgcctactcc cattaggact
       61 aatctaaaat tattattaaa tatttcatct actactctag agataccagt tattcgattt
      121 aaatcaccaa caccatcata tccagaatct cttgacgcat attctaaaag accttgaatt
      181 gtgaaattat cagcatagct ttgtccatta tcgttcaatg taataaatat tgttataaaa
      241 aaggataata ctatcgacac aagtattacg catgataata tcttgatcac tactttaaa
//
LOCUS       DAZKNM010000428          268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000428 DAZKNM010000000
VERSION     DAZKNM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.461"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>268
                     /locus_tag="ACG8M9_003010"
     CDS             <1..>268
                     /locus_tag="ACG8M9_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0379672.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMT"
BASE COUNT          111 a           33 c           57 g           67 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgact
//
LOCUS       DAZKNM010000429          266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000429 DAZKNM010000000
VERSION     DAZKNM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.462"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACG8M9_003011"
     CDS             complement(34..>266)
                     /locus_tag="ACG8M9_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0379673.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAZKNM010000430          243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000430 DAZKNM010000000
VERSION     DAZKNM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.466"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          101 a           35 c           39 g           68 t
ORIGIN      
        1 aaagagtata cgaacaaacg ttctttttgt aaaggtggaa tttattatac taaataaata
       61 ggtgaacaag tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa
      121 acaaaaagaa atcctattat gataacattt cttataataa caaacacaac agaagacgtg
      181 taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt
      241 ttg
//
LOCUS       DAZKNM010000431          239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000431 DAZKNM010000000
VERSION     DAZKNM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.470"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..222
                     /locus_tag="ACG8M9_003012"
     CDS             <1..222
                     /locus_tag="ACG8M9_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0379674.1"
                     /translation="LKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRV
                     AYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT           84 a           24 c           51 g           80 t
ORIGIN      
        1 ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc atataagggt
       61 gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata tgcgggcaca
      121 acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt gttatgtgga
      181 acgcatgaaa acttttatga acagttgaaa aaattaatct agtgatttgt tgtcgttct
//
LOCUS       DAZKNM010000432          237 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000432 DAZKNM010000000
VERSION     DAZKNM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.471"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..216)
                     /locus_tag="ACG8M9_003013"
     CDS             complement(<1..216)
                     /locus_tag="ACG8M9_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0379675.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT           62 a           44 c           44 g           87 t
ORIGIN      
        1 aaaaatgatt tcggttctcc cacaacgtcg cttattttct ttgtattgtt caaagtattc
       61 aagtgctgtt ccaccacatt tgagaaagtt atagacattg taaattgttt ggcgtccacg
      121 tttaagctgc ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat
      181 tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtat
//
LOCUS       DAZKNM010000433          237 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.472, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000433 DAZKNM010000000
VERSION     DAZKNM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.472"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          110 a           33 c           21 g           73 t
ORIGIN      
        1 ctaccagaaa aacgtatgaa taaaatttag ttttaaaatt aaagataatt aagtactacc
       61 agtgtcaaaa caaaaaataa attaagaact accttatgca tataaaacat actaccatta
      121 ctagtaatat atactacctt atttcagtag tatatattat attattctta taagacaagg
      181 ctttaggcta gaaaacaata gattatatac tactaaaaaa tacaataagt actacca
//
LOCUS       DAZKNM010000434          233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000434 DAZKNM010000000
VERSION     DAZKNM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.473"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZKNM010000435          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.474, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000435 DAZKNM010000000
VERSION     DAZKNM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.474"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>232)
                     /locus_tag="ACG8M9_003014"
     CDS             complement(<1..>232)
                     /locus_tag="ACG8M9_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0379676.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DAZKNM010000436          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.denovo.478, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000436 DAZKNM010000000
VERSION     DAZKNM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.denovo.478"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG8M9_003015"
     CDS             <1..>224
                     /locus_tag="ACG8M9_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0379677.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKNM010000437        13895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.061, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000437 DAZKNM010000000
VERSION     DAZKNM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.061"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..2933)
                     /gene="uvrA"
                     /locus_tag="ACG8M9_003016"
     CDS             complement(117..2933)
                     /gene="uvrA"
                     /locus_tag="ACG8M9_003016"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0379678.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(2943..4937)
                     /gene="uvrB"
                     /locus_tag="ACG8M9_003017"
     CDS             complement(2943..4937)
                     /gene="uvrB"
                     /locus_tag="ACG8M9_003017"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0379679.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            5196..6698
                     /locus_tag="ACG8M9_003018"
     CDS             5196..6698
                     /locus_tag="ACG8M9_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0379680.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            6700..7437
                     /locus_tag="ACG8M9_003019"
     CDS             6700..7437
                     /locus_tag="ACG8M9_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0379681.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7608..8801)
                     /locus_tag="ACG8M9_003020"
     CDS             complement(7608..8801)
                     /locus_tag="ACG8M9_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0379682.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(8880..9086)
                     /locus_tag="ACG8M9_003021"
     CDS             complement(8880..9086)
                     /locus_tag="ACG8M9_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0379683.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(9079..10221)
                     /locus_tag="ACG8M9_003022"
     CDS             complement(9079..10221)
                     /locus_tag="ACG8M9_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0379684.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            10733..11368
                     /locus_tag="ACG8M9_003023"
     CDS             10733..11368
                     /locus_tag="ACG8M9_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379685.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            11522..11599
                     /locus_tag="ACG8M9_003024"
                     /pseudo
     CDS             11522..11599
                     /locus_tag="ACG8M9_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            11913..11960
                     /locus_tag="ACG8M9_003025"
                     /pseudo
     CDS             11913..11960
                     /locus_tag="ACG8M9_003025"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11976..13895
                     /gene="tet(M)"
                     /locus_tag="ACG8M9_003026"
     CDS             11976..13895
                     /gene="tet(M)"
                     /locus_tag="ACG8M9_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0379686.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         4045 a         2796 c         2627 g         4427 t
ORIGIN      
        1 ataccaatca gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa
       61 acaagcaaaa attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat
      121 accattattt ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct
      181 ggtgttccag tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg
      241 atataatctg ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca
      301 gcatctacta atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct
      361 gtcgttggtt catctagtat atagaaattc tttccattag agattttgtg caattcactg
      421 gcaagtttca tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc
      481 acataaccta agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc
      541 ttgaagaatt ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct
      601 ttgtaatgca cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga
      661 acatagacat caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct
      721 tcacaacgac cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt
      781 gcctcgttcg tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct
      841 ggattacttc tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata
      901 ctttcgtagc cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt
      961 tgcgctaacg atttctttaa gatactattg accaatgtac tctttccaga accagacaca
     1021 cctgtgactg ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt
     1081 tctgaagcac cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca
     1141 gggattgatt ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact
     1201 tcttcaggag tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg
     1261 tcaatcaaat aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg
     1321 ttgccaagat cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc
     1381 aatccgatcg atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt
     1441 gtagccaatc tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa
     1501 gttagatagt ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa
     1561 atcggtttgg caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact
     1621 gctttttgaa tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat
     1681 agagcttggg gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa
     1741 agacgcattt gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc
     1801 ttcaataccc cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa
     1861 tggaaatgga aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat
     1921 tcttcgaatg gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga
     1981 ggataatact gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc
     2041 gttctatctg gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag
     2101 tctggacaag ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca
     2161 gtaaatccgc aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc
     2221 acgtcaacca gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg
     2281 cgagaacgaa tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt
     2341 ttgtttttct ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg
     2401 cggacgtatc cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt
     2461 accacgatcg gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct
     2521 gccatttgat ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt
     2581 cctacacgtg caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc
     2641 gaccgaggat ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg
     2701 atgctatcta cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg
     2761 ctctctacgt atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt
     2821 ccagaaccgg acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata
     2881 tttttcaagt tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc
     2941 tcctactttg aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa
     3001 tccaaagctt ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt
     3061 tcttccttcg tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat
     3121 tctttcgtaa tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca
     3181 tgttcttcat tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga
     3241 cgcatagaat ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca
     3301 gctcgtccaa tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca
     3361 tccaagatag caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt
     3421 ccaactaaca catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc
     3481 gttttgatat cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg
     3541 aggtcttctg ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact
     3601 cgttcattta tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc
     3661 actgggtcaa gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca
     3721 tgttcatatg gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac
     3781 tcttctaacc gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact
     3841 agcatctgtt ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg
     3901 tgcgattcgt ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc
     3961 ggttcacctt ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag
     4021 cccatttcta acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc
     4081 agaagtttgt tttcatttcg caagacagtt aaacgctgtt ctaattcctc tttgatattc
     4141 gctacggcat gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct
     4201 acgtgttccg tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg
     4261 acctcatctc caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc
     4321 tccacgacat caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg
     4381 aattggatac tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga
     4441 atcgaaacta cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg
     4501 gatgcaatga cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat
     4561 ttgtcgatct catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca
     4621 taagcttcag gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg
     4681 aaaaattcct tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc
     4741 agtgttggtt tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca
     4801 gtcgcaccta ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta
     4861 atcgcttcag gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag
     4921 gtatctctct caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca
     4981 ataaaaagaa aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata
     5041 ctttcgccac ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa
     5101 ctcgcatttt tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta
     5161 taataataaa atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact
     5221 catttgtttt cttaatgaca ggacttgtga cacttgcgtt tggtcatgtt gcacaagctg
     5281 acaacactac aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa
     5341 aagacgtata cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa
     5401 agggcgagta cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat
     5461 ttaacatccg aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac
     5521 aagcagacgg aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt
     5581 tttctgattc ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga
     5641 tcaagtctta tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg
     5701 ctgaattttt aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg
     5761 ctgatcaatt gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc
     5821 cggtaatcgg ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat
     5881 ctggcggaaa atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt
     5941 tcaatgaagg attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat
     6001 atgtagcgga tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc
     6061 ttggacttat ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc
     6121 taactttgat ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg
     6181 tagcaccaat acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa
     6241 ttccaatgat ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta
     6301 cgattcctga cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg
     6361 cagagattgt tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa
     6421 gtttaggtct ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca
     6481 tcatgatccc atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt
     6541 ccgcaatcgg tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac
     6601 aaagtacgta tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga
     6661 caaaattagc aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt
     6721 gagcatttag tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc
     6781 aacgaaggag atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg
     6841 cgttgcttga atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac
     6901 ttgatggata aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa
     6961 cactttaacc tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac
     7021 ctgaagaaag aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt
     7081 ggtctagaag ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt
     7141 gtggctattg ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact
     7201 tctgcactcg atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa
     7261 caaggtatga cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat
     7321 cgcgtcatgt tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt
     7381 gaaaatccgc aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaaaat
     7441 aaattatata tgtctttttg actatctttt tatatcttct taaatgtccg caaagcctta
     7501 ttttataggc tctacgggca ttttgctttt gtggtaaacc tcacatatct aggtctatct
     7561 tcttatattt tcactatcaa gcgtggttaa aatcgtggta aatacttcta tgcaattaga
     7621 cgctgaacct ctgattttgc ggtatctatg gacgcatgag cataccagtt cattgtgata
     7681 ctgatatttg aatgccccat gatatactgt aaatcttttg ggttcatgtt cttgcttgcc
     7741 agtcttgtgc agaatgtatg gcgtagcgta tgcggtgtga tatgtggcaa tggattgtcc
     7801 ttgtggtgct tgttgtattt ctttaccata cggataaata aggcgttata atcaatcgca
     7861 actttgggct tgcccttatg attgacaaat aggaagttgc cccgtccgtc tatcacaaat
     7921 ggttctgcct ttggacgttt tttcataacc tgttgaaatg cctgtatcgt ttctttgctt
     7981 aatggtactt gccttattcc gctttttgtt ttaggcgttt caatataata gccctgttcc
     8041 ttgctcttta gtaactggtg gtcgataatc acaacttcat tcttgaaatc aatgtcggct
     8101 actgtcagtc cgcacagttc cgaaatacga agtcctgtct ttaacagtat cagcacatca
     8161 tcataatgct tgtgatacac attgtccgtc ttgatgaatg agagtaaggc ttgttcctgt
     8221 tcctctgtca atgcgacttt ctttttggta tcattttcta ggacttcact caacttgaaa
     8281 tcaaagggat ttttcctcac ataatcatct tgaatggcta tgtagaatga cgcttttaac
     8341 gagcgtttat ggttgttaat ggtattgtag gaaaagcctt tgtctttcat gcgtaacgcc
     8401 cattccttag cgtcagaggg tttgattgta tcaatgctcc tagcacctaa cttgtcctct
     8461 ttcaataacc gcatgagttg ttcccgttgt ttctgtgtgc ttttcttcac atttgccctc
     8521 tgtgcgttct gtttggcata aagttggcaa agcgtcattt tcttgccagt gctgtcgata
     8581 ccgtcctcaa tatcccgtct tatctgctgt tccagttctc gaagtgaggg tttttcccgt
     8641 tttccctttg gtgtcgggtc tgtgggtgtc aatctccaag catacacata ttttgtgttt
     8701 ccaaatgcgt ctacatattt atataagtat tttctgtctg ttcgttggct ctctccagta
     8761 tgcaggatac gacctttgtt atcccgtctt ttttctttca tggtgtctgc tcctttcctt
     8821 gttggaaaga gccttgatat gacttgtgtt catcataaca catacaaggc tcatttgcat
     8881 tagatagcgt ccaatttatc aatgacctgt tcaaactgtc ggcgtttaat ctgtatgcgg
     8941 ttgccattca taatgagcca gccagcgtcc ttgttttcct ctgccaatcg tcttaacttg
     9001 ttttctccga tacggaaata ctttgacgct tcttcaatgg taagggtgta tttttcccat
     9061 acgggaatat cctgtgcatt actcataaaa caccccctca ctttcgatta cgtctgctat
     9121 gtccgtacac atctgttcca caagcatttc ctgcttttca gagaggtggg tacattcatc
     9181 aatcattttc atgtagtttg agcctgtcag cctgtcgatt tcctctatca tcacgattga
     9241 cggcatgacc tgtctttcca gccattgcag agtgcgttcc atattgacag gtacggggtt
     9301 catttcaaaa ggtaaactgt catgggaaat gaatatctcc cacagcggat agtcgggaaa
     9361 ttgcacaaaa tcagtaataa ggtaaaacac cagcccatga gggttatatg tcaataacaa
     9421 ttcctcaact gcctgtactg cttttgtacc acgcagacga atttcaaaac gattgataat
     9481 atccgtatgt ttctttttgg ttgcctgttc cttttgtttt tcatacaggc agaagtattt
     9541 tgtgcttgcc tttgaaccga tataaagggt gcttgctaaa ttacggtttt ttccgcttag
     9601 ttttccaccc tgcacatttt cataattttt acagcgacaa tccgcaccgc catttctgta
     9661 tttttcagac aaaacgggaa tatccaacag accacacata tcattgattg ccaagtcaaa
     9721 acgtcggata acaccgtcac agtcaagaca gcggtttaaa aatgaatacc aatctctgtt
     9781 ctgtgcctgc aagacatatt ccatgttgcg tgagcctgcc ccttttaatt ccaagaatac
     9841 gcccatgtgt tcgtcatcag aagccatgac cttgatttct ccgtaggcgt aatgctcttt
     9901 atagccataa tacccatagt cataatggat aaaataatct gctttcatgc caagcacctt
     9961 tcggataatt tccaatgcgt cagtcgttgg ataacggaca gacagataat caacgagcag
    10021 ataaaaaggc ttatcgcaga tataataatt cagacaacgc aggataatat tttgtgtttc
    10081 cttgctggcg ttggcttttc cgttctcaaa gcggttgata ctatatcttg atacatgggt
    10141 aagacttgca agttctcctt gtgaaagacc gcttgcaaga cgtttgttct tcatttcaag
    10201 tgcaaattct aaattgttca ttgtattcct ccaatcccac aatagccttg aaatacctgt
    10261 aaacctctat tcaaaaagtg tgatatgcac tatcttctta aaacttctca ttactactca
    10321 caactttggg attgtatggt ttttgacttg tcttttgtga aatagtgccc ccctgttaga
    10381 taacgggggc acagacaagg ctcgcaggct cgccagccga caaagcattt cttccccttg
    10441 acacaaggtg tcatggcggt aagcctgaga ggcttccgcc accgtgtcag aaatactttg
    10501 tcagcaagtt ctgttttgcc ttttctaaca agttccattt caaccgatat tcatatctac
    10561 ggatttaatc agataggtat ttctttttga atgtttttat atttcattct attgtgctta
    10621 ttcgattaca agaacatagt aattcttata tgcttatttg tcaatatctt tttttgcctt
    10681 tacaaatcat attaagcatg atagacttat aatagcgaag atataacgag atatggaggg
    10741 ttatgcaatg aaaataagcc atttaggaaa taatatacag accataagaa aatttagggg
    10801 aatgaaacaa caggaacttg cggacaaaat cggtatcaat atgcagagcc tttccaagat
    10861 tgaaagaggg ttgaactatc ctgcctatga aacgctggaa aagataatgg aagtgctgga
    10921 cgttacgccc aatgaattgt tgtcgggaga atggaagtat gtcaatcaat ctgaaaagga
    10981 agtctgtcag tttttaagag ccgaagaacg tctaaatgca gaactgaaac atggacacta
    11041 tgataacttc tttgacagcg aggaagaatg gctggaatat gagttagaaa agttacggga
    11101 atatattaca gactatatca acgggaaaag cattgaagcg tccgaccttt acccaatcaa
    11161 agaatttatc cagcatttga agtttcaaaa attattagac cgctatgatg atttatacag
    11221 tatggatatg tttggggaaa gcatagaggg acataagtat aagacacctt atcaagtcgt
    11281 gaaaatgata aacccgaact caaaagaaga tatggaactg ttacgagaag tattaaggaa
    11341 taacaatttt gatgatgagg acgagtaact tttttcctct tgcgagaaga tacagcaaac
    11401 aaaaagcccg atcaagagtg caaagcacca ccaatggtgg caaagctctt gacagggctt
    11461 tttcttttct gttgtgggca atcaagagga agaaagggat aaaatctgat ttgtggtaaa
    11521 tcaggcaaag gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa
    11581 ttggaagatt atagaataac aaatattggt acattattac agctattttg taatcacgta
    11641 ctctctttga taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta
    11701 agtatctatt taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa
    11761 tttgatagtg ggaacaaata attggatgtc cttttttagg agggcttagt tttttgtacc
    11821 cagtttaaga atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt
    11881 gtatgcctat ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt
    11941 atgccctttt gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt
    12001 tagctcatgt tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag
    12061 cgattacaga attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag
    12121 aacgtcagag aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga
    12181 aggtgaacat catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat
    12241 tatcagtttt agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa
    12301 ctcgtatatt atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata
    12361 agattgacca aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt
    12421 ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact
    12481 ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat
    12541 atatgtccgg taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc
    12601 agaattgttc tctgttccct ctttatcatg gaagtgcaaa aagtaatata gggattgata
    12661 accttataga agttattact aataaatttt attcatcaac acatcgaggt ccgtctgaac
    12721 tttgcggaaa tgttttcaaa attgaatata caaaaaaaag acaacgtctt gcatatatac
    12781 gcctttatag tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa
    12841 taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt
    12901 attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag
    12961 atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa
    13021 ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct
    13081 cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt
    13141 ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg
    13201 tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag
    13261 aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg
    13321 tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact
    13381 taaatcaatc gtttcaaaat gcagttatgg aggggatacg ctatggctgt gaacaaggat
    13441 tgtatggttg gaatgtgacg gactgtaaaa tctgttttaa gtatggctta tactatagcc
    13501 ctgttagtac cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa
    13561 aaaaagctgg aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg
    13621 aatatctttc acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc
    13681 aattgaaaaa taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat
    13741 atcgtagtga tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag
    13801 ggtaccatgt tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag
    13861 ataaagtacg atatatgttc aataaaataa cttag
//
LOCUS       DAZKNM010000438         8163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.121, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000438 DAZKNM010000000
VERSION     DAZKNM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.121"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG8M9_003027"
     CDS             complement(134..577)
                     /locus_tag="ACG8M9_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0379687.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG8M9_003028"
     CDS             complement(795..2135)
                     /locus_tag="ACG8M9_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0379688.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG8M9_003029"
     CDS             2404..3300
                     /locus_tag="ACG8M9_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0379689.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG8M9_003030"
     CDS             complement(3450..4391)
                     /locus_tag="ACG8M9_003030"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0379690.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG8M9_003031"
     CDS             4517..5638
                     /locus_tag="ACG8M9_003031"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0379691.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8M9_003032"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8M9_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0379692.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG8M9_003033"
     CDS             7465..7992
                     /locus_tag="ACG8M9_003033"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0379693.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2710 a         1514 c         1606 g         2333 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tag
//
LOCUS       DAZKNM010000439         5753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.150, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000439 DAZKNM010000000
VERSION     DAZKNM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.150"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..736
                     /locus_tag="ACG8M9_003034"
     CDS             272..736
                     /locus_tag="ACG8M9_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0379694.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            720..1349
                     /locus_tag="ACG8M9_003035"
     CDS             720..1349
                     /locus_tag="ACG8M9_003035"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0379695.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1703..3181
                     /gene="msr(C)"
                     /locus_tag="ACG8M9_003036"
     CDS             1703..3181
                     /gene="msr(C)"
                     /locus_tag="ACG8M9_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0379696.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3228..3674
                     /locus_tag="ACG8M9_003037"
     CDS             3228..3674
                     /locus_tag="ACG8M9_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0379697.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3873..4388)
                     /locus_tag="ACG8M9_003038"
     CDS             complement(3873..4388)
                     /locus_tag="ACG8M9_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0379698.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4505..5707
                     /locus_tag="ACG8M9_003039"
     CDS             4505..5707
                     /locus_tag="ACG8M9_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0379699.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1783 a          957 c         1222 g         1791 t
ORIGIN      
        1 aaaggcgatt tcggaaattt ttggcggtgt aaaattatct actgcagaag agctatcttc
       61 tatagtttga taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt
      121 ccgttcattc tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta
      181 ttacacagct gattctttga cttttttctt tttttattct attattgaca agtcaatcgt
      241 tgacgagtca attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt
      301 atatcaaatc aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat
      361 aagtcttact agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag
      421 cagtttgcaa gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt
      481 ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt
      541 agcgctaaca gaaaaagccc agaagaaaat caagcattgt gaaatggacc ataaagatac
      601 aggttccgtg ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt
      661 agataaattg aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa
      721 tggaacaaaa aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg
      781 atgaaacata caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg
      841 caccttcttc tgtcaatatg aaaccatggc gctttgtggt cgctgaaagc gaggaagcaa
      901 aagaaatctt gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa
      961 tggtcatgat ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tacaaccaag
     1021 cttacgaatc agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc
     1081 cttattacaa gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct
     1141 tagcggctat gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg
     1201 gtggattcga agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc
     1261 cagttattat tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag
     1321 caccagaaaa gatcactatg ttcgaataaa aggttttcag ttactattca gtagctattg
     1381 ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc
     1441 gatgaaatta cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa
     1501 acaacaattt catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag
     1561 ctatgacaga tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat
     1621 gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac
     1681 atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt
     1741 cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg
     1801 tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg
     1861 tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca
     1921 attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt
     1981 aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt
     2041 gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct
     2101 agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt
     2161 ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa
     2221 agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt
     2281 ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga
     2341 aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa
     2401 gaaaaagcaa caaatcagac tggatcggtt gtcttcctct aaacaaaaag acagtgtaca
     2461 aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac
     2521 aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca
     2581 cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact
     2641 ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg
     2701 ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc
     2761 tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc
     2821 catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct
     2881 gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg
     2941 agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt
     3001 agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca
     3061 aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc
     3121 tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta
     3181 aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata
     3241 aaaaagaagg aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg
     3301 cgtataattt atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc
     3361 ctggattcgt ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa
     3421 ttgcctttaa actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat
     3481 gcttgatggt tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag
     3541 gaatacctga tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg
     3601 ctttaccgtt gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc
     3661 aaagtaatga atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat
     3721 agtctcttga atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt
     3781 tcggatcgct ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca
     3841 atagatattt ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct
     3901 cttacttgct gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca
     3961 acataacgtc caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt
     4021 ttttgcatga ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt
     4081 ttatcgagcg tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg
     4141 ggagtttcct ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag
     4201 atcttttttt gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac
     4261 ttggcaagcg ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc
     4321 ttcaattcat acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa
     4381 gtattcataa aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc
     4441 acgttttaca tgtgcatatt gacatgtaaa ataaatcatg tatgatgcta atagagagaa
     4501 agggatggaa atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa
     4561 ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg
     4621 tctagtagac ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac
     4681 gaccctgtta tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt
     4741 cttatctagt cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt
     4801 attcgctggt cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg
     4861 ctggggtgtc gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt
     4921 agttggtaac acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc
     4981 tgttggccct ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg
     5041 ggtagcattt ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga
     5101 gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact
     5161 tcgttcagtt ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta
     5221 tgcgccattt ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg
     5281 gggtgtgctt ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac
     5341 gacttatcgg acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg
     5401 agctggaact tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca
     5461 aggaattatc aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa
     5521 cgttgcttcg tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat
     5581 tgcaggtgtg attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat
     5641 tgttcttatc agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga
     5701 agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DAZKNM010000440         4802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.173, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000440 DAZKNM010000000
VERSION     DAZKNM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.173"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8M9_003040"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8M9_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0379700.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /gene="vanS"
                     /locus_tag="ACG8M9_003041"
     CDS             763..1917
                     /gene="vanS"
                     /locus_tag="ACG8M9_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFQ0379701.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSNLENKYDLNHLNAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8M9_003042"
     CDS             2132..3100
                     /locus_tag="ACG8M9_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0379702.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8M9_003043"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8M9_003043"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0379703.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8M9_003044"
     CDS             4130..4738
                     /locus_tag="ACG8M9_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0379704.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1483 a          921 c         1118 g         1280 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtaattt ggaaaacaaa tatgacttaa atcacctgaa cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAZKNM010000441         4802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.174, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000441 DAZKNM010000000
VERSION     DAZKNM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.174"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8M9_003045"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8M9_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0379705.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /gene="vanS"
                     /locus_tag="ACG8M9_003046"
     CDS             763..1917
                     /gene="vanS"
                     /locus_tag="ACG8M9_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFQ0379706.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSNLENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8M9_003047"
     CDS             2132..3100
                     /locus_tag="ACG8M9_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0379707.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8M9_003048"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8M9_003048"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0379708.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8M9_003049"
     CDS             4130..4738
                     /locus_tag="ACG8M9_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0379709.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          921 c         1119 g         1280 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtaattt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAZKNM010000442         4802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.175, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000442 DAZKNM010000000
VERSION     DAZKNM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.175"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8M9_003050"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8M9_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0379710.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8M9_003051"
     CDS             763..1917
                     /locus_tag="ACG8M9_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0379711.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8M9_003052"
     CDS             2132..3100
                     /locus_tag="ACG8M9_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0379712.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8M9_003053"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8M9_003053"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0379713.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8M9_003054"
     CDS             4130..4738
                     /locus_tag="ACG8M9_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0379714.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAZKNM010000443         3067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.232, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000443 DAZKNM010000000
VERSION     DAZKNM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.232"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..527
                     /gene="dfrG"
                     /locus_tag="ACG8M9_003055"
     CDS             30..527
                     /gene="dfrG"
                     /locus_tag="ACG8M9_003055"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0379715.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(599..2551)
                     /locus_tag="ACG8M9_003056"
     CDS             complement(599..2551)
                     /locus_tag="ACG8M9_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0379716.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2558..2794)
                     /locus_tag="ACG8M9_003057"
     CDS             complement(2558..2794)
                     /locus_tag="ACG8M9_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379717.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          920 a          553 c          504 g         1090 t
ORIGIN      
        1 gtaaattagt taaaaaggct gtgttataaa tgaaagtttc tttgattgct gcgatggata
       61 agaatagagt gattggcaaa gagaatgaca ttccttggag gattcccaag gactgggaat
      121 atgttaaaaa tactacaaag ggacatccga taatattagg taggaagaac cttgaatcaa
      181 tcggaagagc cttacctgac agaagaaata ttattctgac gagagataag gggtttacct
      241 ttaatggttg tgaaattgtt cattcaatag aagatgtttt tgagttatgt aaaaacgaag
      301 aagaaatttt tattttcgga ggagaacaga tttataattt gtttttccct tatgttgaga
      361 aaatgtacat cacaaaaata catcatgaat tcgaaggaga tacttttttt ccagaagtga
      421 attatgagga atggaatgag gtatttgccc aaaaagggat aaagaatgat aaaaatccgt
      481 ataactacta ttttcatgta tatgaaagaa aaaacttatt gagttaacgg gtgcgttaat
      541 tgaagaagaa cagtaaagat aaagcacaag agcttgggat tactccaagc tctttttgtc
      601 atttgaaata aattatatct ttccaatcaa attgtttatc tgtaatccct aatctttgag
      661 caggtgtcaa aaccttttta tccgctgatt taaaagggcg acaaaagttg tagtaagttc
      721 ttagaattgt aatcgcaaat tgagcatact tcggattgaa gttagcgtaa atatagctct
      781 tcttatcacc acgagctgtt gttaatggtc tttcaagaga tgatatataa cgtcttatct
      841 gttgaataaa gctattggtc gaatggtcat taacatttaa tagcatcttt gcgatttcgt
      901 ttggttccag agcagataaa tccgttctac aatcaactga ataaaatcct ttatccttag
      961 tggctaatgg atgtttgata gggttatcgg catatttgcg atatctttct ccatctttac
     1021 taatagcctc ttcgtggaat tgatgacttg ttagtaattc agttagataa tgagatgcca
     1081 aagtccgtag aaactttgtt ttgaatcctc tgatatctcc ccaatcgagc agttctgctt
     1141 ttgcttgtcc gaattctttt aagcattgtt ttctgctttt cgtcttgtta acttggcaaa
     1201 taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa tactctataa aaggcgtttc
     1261 taatagagaa gtcatcatca ctaatcattc gccattcgtc ggcattaacc atttgtttaa
     1321 ttaaccaata gtgagcagtt gtagtataag gagcatcaac atttaaacca tcgacaaatc
     1381 tactcctatt agtaattttc cctaattcgt gaagataagc attcctattt tcgctatcgt
     1441 tagcggatgg ttcttgtgga taataagacc aatctaacct atcattttta cgactaaaag
     1501 tattgagatg gtcttctttg aattttcttg tatcttcatt taattcatcc attgaaatat
     1561 tccaatcata ggcaacatca gaacggaaca catatcttga taagacctca gcagatacaa
     1621 ctatatacgt ttgcatatta aggtcttcaa accccgtata tttctttgaa ccttgtcctt
     1681 tcttacggac gttatttagg tagtagtgca tcttgtccgt attaagccac atctcgttaa
     1741 atttctttgt ttgaaggggt tgtgtctcat atcgttctaa aaactccaaa caacgcctat
     1801 acagccactc cagcttatga tagtaggttc caactccaat tcctaaaata tcacaagtac
     1861 gactgactga aacccttcca acaaccatct tagcgaacat tggaagaatg gtgttctttt
     1921 gttggtgata agtagtggtt tcttctcttt ttggtagaac attagtgaac ttcttacacg
     1981 ccttacattg atagcgttga gatttgcccc tacttttccc ccttttgtaa aattgcttcg
     2041 gctcattgaa tggggttgat tcttcttccg agcagccttc tttatgaaat tgataatctg
     2101 gctcaacatc ttggatgcta ttgatttcta taaggcgttt aatttcttca acaacagacc
     2161 aattagataa aggggttact gtacagtttt gcgatacacc tctgccaata ggattgtcat
     2221 tgcagtacat acctttatga cctttatctt tggaactacc agtcatttta tatcgacttg
     2281 gtttcccttt tacatctttg aatttgtgct gttctttccc aaaattacaa caaaacggat
     2341 tgatgcagta attatattga atgggatatt cagaaccgtt ccatttaaaa gacgttggca
     2401 agaacaacat tttgttatac cttgttgcaa atcgttttgg agttaataaa tcatattgtt
     2461 tagccctatc ttttaattct tcgtttgaaa tcggaatttt aacatctaca atttcatctt
     2521 ccttcgtggc taatctttta agcttggtca ttgtctatca acccactcta tctttgatac
     2581 ccattttttc aacaatccgc ttattacttc gcttgttggc aatccattca ataaacttct
     2641 tatcgtctaa aatgttcaat aagaattcat ccactgcttc gctttcagta cgctctgcat
     2701 attcagcgta ttgcttaact atcaccctta cttgctctga tatttcccaa tccacagact
     2761 tagcgttctt attttttggc tcaagaaatt gcataggaaa atgcacccct ttttgcttag
     2821 taaattgtaa ccattttaac acaccaattt gcattcctac aataattatt tcacgttcgc
     2881 atttttatat acattttaac ttacattttg tggtgtaaat taacaacaaa cgttgattta
     2941 acaataaaaa acgagtatca aattcagttg caatttgata ctcatttagg tttacatttt
     3001 cactttttgt actccaaaac tgaactactt aaatctatac tgctgcctct tttttttagt
     3061 acatgtc
//
LOCUS       DAZKNM010000444         2323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.259, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000444 DAZKNM010000000
VERSION     DAZKNM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.259"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..932
                     /locus_tag="ACG8M9_003058"
     CDS             21..932
                     /locus_tag="ACG8M9_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0379718.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1085..1570
                     /gene="vanZ-A"
                     /locus_tag="ACG8M9_003059"
     CDS             1085..1570
                     /gene="vanZ-A"
                     /locus_tag="ACG8M9_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0379719.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2089..>2323
                     /locus_tag="ACG8M9_003060"
     CDS             2089..>2323
                     /locus_tag="ACG8M9_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0379720.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQ"
BASE COUNT          855 a          333 c          433 g          702 t
ORIGIN      
        1 agttaaggag gagactgaaa atgaagaagt tgtttttttt attgttattg ttattcttaa
       61 tatacttagg ttatgactac gttaatgaag cactgttttc tcaggaaaaa gtcgaatttc
      121 aaaattatga tcaaaatccc aaagaacatt tagaaaatag tgggacttct gaaaataccc
      181 aagagaaaac aattacagaa gaacaggttt atcaaggaaa tctgctatta atcaatagta
      241 aatatcctgt tcgccaagaa agtgtgaagt cagatatcgt gaatttatct aaacatgacg
      301 aattaataaa tggatacggg ttgcttgata gtaatattta tatgtcaaaa gaaatagcac
      361 aaaaattttc agagatggtc aatgatgctg taaagggtgg cgttagtcat tttattatta
      421 atagtggcta tcgagacttt gatgagcaaa gtgtgcttta ccaagaaatg ggggctgagt
      481 atgccttacc agcaggttat agtgagcata attcaggttt atcactagat gtaggatcaa
      541 gcttgacgaa aatggaacga gcccctgaag gaaagtggat agaagaaaat gcttggaaat
      601 acgggttcat tttacgttat ccagaggaca aaacagagtt aacaggaatt caatatgaac
      661 catggcatat tcgctatgtt ggtttaccac atagtgcgat tatgaaagaa aagaatttcg
      721 ttctcgagga atatatggat tacctaaaag aagaaaaaac catttctgtt agtgtaaatg
      781 gggaaaaata tgagatcttt tattatcctg ttactaaaaa taccaccatt catgtgccga
      841 ctaatcttcg ttatgagata tcaggaaaca atatagacgg tgtaattgtg acagtgtttc
      901 ccggatcaac acatactaat tcaaggaggt aaggatggcg gaatgaaacc aacgaaatta
      961 atgaacagca ttattgtact agcacttttg gggtaacgtt agctttttaa tttaaaaccc
     1021 acgttaacta ggacattgct atactaatga tacaacttaa acaaaagaat tagaggaaat
     1081 tatattggga aaaatattat ctagaggatt gctagcttta tatttagtga cactaatctg
     1141 gttagtgtta ttcaaattac aatacaatat tttatcagta tttaattatc atcaaagaag
     1201 tcttaacttg actccattta ctgctactgg gaatttcaga gagatgatag ataatgttat
     1261 aatctttatt ccatttggct tgcttttgaa tgtcaatttt aaagaaatcg gatttttacc
     1321 taagtttgct tttgtactgg ttttaagtct tacttttgaa ataattcaat ttatcttcgc
     1381 tattggagcg acagacataa cagatgtaat tacaaatact gttggaggct ttcttggact
     1441 gaaattatat ggtttaagca ataagcatat gaatcaaaaa aaattagaca gagttattat
     1501 ttttgtaggt atacttttgc tcgtattatt gctcgtttac cgtacccatt taagaataaa
     1561 ttacgtgtaa gatgtctaaa tcaagcaatc tgatctttca tacacataaa gatattgaat
     1621 gaattggatt agatggaaaa cgggatgtgg ggaaactcgc ccgtaggtgt gaagtgaggg
     1681 gaaaaccggt gataaagtaa aaagcttacc taacactata gtaacaaaga aagcccaatt
     1741 atcaatttta gtgctgagga attggtctct ttaataaatt tccttaacgt tgtaaatccg
     1801 cattttcctg acggtacccc cactagcagc catgcttttt ttgaatcttt gtaggacgtt
     1861 ttaaagagaa ttacccactt taaaattaaa catgcgctta gaatcgcttt aggaaacacg
     1921 atccagtcca ataatcgtcg ataaaaactt ttgaaaaagg ttggtgaaat tacctacttt
     1981 tggaataatc acaaatcaca agtgattaat cacttgttta ttaagatatt aaaagctata
     2041 atttaaataa agcgtgaatt ttattacaca aaaagagggg ggagaaactt ggaactagca
     2101 tttagagaaa gcttaaaaaa gatgagaggt accaaatcaa aagaaaaatt ctcccaagaa
     2161 ttagaaatga gtagatcaaa ttattcacga atagaatcag gaaaatcaga tccaaccata
     2221 aaaacactag aacaaattgc aaagttaact aactcaacgc tagtagtgga tttaatccca
     2281 aatgagccaa cagaaccaga accagaaaca gaatcagaac aag
//
LOCUS       DAZKNM010000445         1308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.315, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000445 DAZKNM010000000
VERSION     DAZKNM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.315"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..758
                     /locus_tag="ACG8M9_003061"
     CDS             <1..758
                     /locus_tag="ACG8M9_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFQ0379721.1"
                     /translation="IEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDD
                     YNKIDLTLLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDC
                     CNEFWNVTPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYK
                     FIERYISEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRN
                     ITKYTRDMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            755..1235
                     /locus_tag="ACG8M9_003062"
                     /pseudo
     CDS             755..1235
                     /locus_tag="ACG8M9_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          453 a          191 c          290 g          374 t
ORIGIN      
        1 atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga
       61 tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta
      121 tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata
      181 attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa
      241 gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg
      301 atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta
      361 aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa
      421 tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata
      481 agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga
      541 tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg
      601 tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa
      661 caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca
      721 catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca
      781 cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag
      841 gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag
      901 ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa
      961 tactgataag ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg
     1021 ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact
     1081 tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata
     1141 attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
     1201 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
     1261 tcgtcttgtg gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKNM010000446         1113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.336, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000446 DAZKNM010000000
VERSION     DAZKNM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.336"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..424
                     /locus_tag="ACG8M9_003063"
                     /pseudo
     CDS             75..424
                     /locus_tag="ACG8M9_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(696..986)
                     /locus_tag="ACG8M9_003064"
     CDS             complement(696..986)
                     /locus_tag="ACG8M9_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0379722.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          342 a          225 c          162 g          384 t
ORIGIN      
        1 atttcctaat agagtataac atagctttaa gcattactta aagataactt aaaagttatc
       61 ttttaaatac ttaattgtta aataccttat ttgatgcaaa agaaatctac gatagtcaac
      121 aaaaaaattt tcattatatc cagaaaaatt tttcttagtt aaggacctat ggattgcagt
      181 aatgcagaat tcgtcgaata aactgccaaa gacatacaat catattgctt ttaaaattga
      241 cgaacaagat atagattcgt ttgtctcaaa aatccaaatg ttaggtttaa ctgtcgaacc
      301 aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac ttttacgact atgataatca
      361 cctcttcgag ttacataccg gtactctcca ggaacgttta aaatcctaca attccactac
      421 ctgagactag taatgactag tctcttttta tggcctctga tccccccatt gagcgaatac
      481 taatagggct tcgaattgat ctgttcaatc tttaagcaat atccctcaaa tgagtttgga
      541 aagtttagtg attcatcaca aatacttaaa tacatgtagt tatatataga ttgaggttct
      601 tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg catccatctt tccaaaacaa
      661 gcggctagga catttttgtc tcagccgctt gttttctagt ttattctttc atcttgtcgg
      721 accttttttc ttgggtatag acttttctca tattctacca caattttggg tttccctaac
      781 acatgatagg atctcacaac aaattgcttt ctattattat aataacctgt aacgactaac
      841 ttcatccctt cgtctacttc agctaggaag ttcaaactat gggaagcaat caaacaattg
      901 accccattta aactgaaacg gatcaacggt ctggcactaa cttgcaaagt acggatctta
      961 tctactgttc catatttttt ctccataacc ttcacctcaa taccattata cgaacataca
     1021 ttccctttgt aaagaagaaa ctctgtttta tctttttgac tttttgtcta ttacctttct
     1081 taaaatcata ttggcacttc ctgcgaagat gaa
//
LOCUS       DAZKNM010000447          820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.364, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000447 DAZKNM010000000
VERSION     DAZKNM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.364"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..754
                     /gene="erm(B)"
                     /locus_tag="ACG8M9_003065"
     CDS             17..754
                     /gene="erm(B)"
                     /locus_tag="ACG8M9_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0379723.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            699..>820
                     /locus_tag="ACG8M9_003066"
     CDS             699..>820
                     /locus_tag="ACG8M9_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HFQ0379724.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFFKFGKLHVTKGNGDKL"
BASE COUNT          307 a          140 c          130 g          243 t
ORIGIN      
        1 cttcaggaga gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
       61 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      121 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      181 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac
      241 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      301 taaaattgtt gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      361 tgaaagccgt gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      421 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      481 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      541 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      601 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      661 gaaacacgcc aaagtaaaca atttaagtac cattacttat gagcaagtat tgtctatttt
      721 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
      781 gaaagttaca cgttactaaa gggaatggag ataaattatt
//
LOCUS       DAZKNM010000448          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.375, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000448 DAZKNM010000000
VERSION     DAZKNM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.375"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..775
                     /gene="aac(6')"
                     /locus_tag="ACG8M9_003067"
     CDS             227..775
                     /gene="aac(6')"
                     /locus_tag="ACG8M9_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0379725.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          270 a          119 c          166 g          220 t
ORIGIN      
        1 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
       61 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
      121 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      181 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      241 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      301 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      361 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      421 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      481 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      541 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      601 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      661 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
      721 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataa
//
LOCUS       DAZKNM010000449          694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3509
            SAMEA115706767-rid22570013.guided.386, whole genome shotgun
            sequence.
ACCESSION   DAZKNM010000449 DAZKNM010000000
VERSION     DAZKNM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706767
            Sequence Read Archive: ERR13196501
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287771.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287771.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:09:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 40 of 93
            Pseudo Genes (incomplete)         :: 48 of 93
            Pseudo Genes (internal stop)      :: 23 of 93
            Pseudo Genes (multiple problems)  :: 15 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706767-rid22570013.guided.386"
                     /isolate="VRE3509"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..394
                     /locus_tag="ACG8M9_003068"
     CDS             <1..394
                     /locus_tag="ACG8M9_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0379726.1"
                     /translation="LYTVFNKQTEKFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMF
                     PKKPIPENTIPISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQL
                     HHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT          243 a           86 c          105 g          260 t
ORIGIN      
        1 tttgtataca gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga
       61 taaaaacttc atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa
      121 agaagaaatg tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc
      181 ttggattgat tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc
      241 tattattacg ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct
      301 gcaacttcat cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca
      361 agatataatt catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa
      421 ttttatacac gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg
      481 cttgtggttt tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt
      541 tttgtgcttg cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta
      601 gtaaataagt ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa
      661 gaacaactag caaaagattt taaagagtta tttt
//
